LOCUS       DAOTIP010000001        12906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_5790_length_12906_cov_3.698078, whole genome shotgun sequence.
ACCESSION   DAOTIP010000001 DAOTIP010000000
VERSION     DAOTIP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5790_length_12906_cov_3.698078"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5138_00005"
     CDS             complement(<1..215)
                     /locus_tag="P5138_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV58987.1"
                     /translation="MESESAIVFGGASGLGEATARRLAEAGHKVMVADLNEEKGVELV
                     GKIGGTFVKADVTDEDAFEALKEIGLG"
     gene            256..1404
                     /locus_tag="P5138_00010"
     CDS             256..1404
                     /locus_tag="P5138_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV58988.1"
                     /translation="MKRANFEPEHQDFRETVGRFVTDEIMPNFEQWEADAIVPRELFQ
                     KAGEKNMLAMSVSEEYGGLGLKDFRFNQIVGEEGYYQGATGAMLGITLHNDVCLPYFE
                     EYCTDEQKERWLPGIVDGSLITAVAMTEPGIGSDLASMSTKAIRNDDHYVVDGSKTFI
                     TNGINADLIITAVKTDPKEKHRGMSIIIIERGMEGFDRGRNLEKVGMHSQDTAELFFS
                     DVKVPVENLLGNEGDGFKYLVSNLAQERMSIAVAAVAAAQAALNWTLDYVKERYAFGS
                     PIGTFQNSRFQMAEIKTEVDIATVYIDRCVEALNEGTLTVEDAAEAKWWSTEMADRVI
                     DKCVQLHGGYGYMLEYPIARAYADNRVTRIYGGTTEIMKEIVGRSMGL"
     gene            1483..1968
                     /locus_tag="P5138_00015"
     CDS             1483..1968
                     /locus_tag="P5138_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HRV58989.1"
                     /translation="MPAARIAKIVSGGQTGVDRAALDAGIAIGIPYGGWCPKGGLAED
                     MPDPPGLLEPYPELTETPSDDVSQRTEWNVRDSDATLIIRPEGSKSPGTDLTIELAAR
                     MNRPCRTVDPESNDAVAEVLAFIESIGPEAVLNVAGPRESGAPGIYEAARDLLTRSLS
                     N"
     gene            complement(2099..2881)
                     /locus_tag="P5138_00020"
     CDS             complement(2099..2881)
                     /locus_tag="P5138_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRV58990.1"
                     /translation="MNFTLEIWRQFDGADETDQGRFETFKVTDINPDASFLEMLDQLN
                     ERLSHEKKRTIAFDSDCREGICGACSLVIDGTPHGPQQVTSCQTYMRDFIDGQTIKVE
                     PFRANTFPVIRDLAVDRGALDRVIQAGGYISTTTGPQPEPNSMPVSPETQEKAMDAAI
                     CIGCGACVAACPNGAAMLFTGAKVTHLNVLPQGQPERESRVIGMVEQMDAEGFGGCTN
                     YGECSRVCPQEISIDVIGMLNREYRKAKVKKKPTPKKAMTTQ"
     gene            complement(2878..4785)
                     /locus_tag="P5138_00025"
     CDS             complement(2878..4785)
                     /locus_tag="P5138_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRV58991.1"
                     /translation="MTVFDSNIPAGPLADKWQNWLDAHTLVGPRNRAGKNVIVIGTGL
                     AGSSCAASLGAQGFKVKSFCFQDSPRRAHSIAAQGGINAAKDYANEGDSAHRLFYDTI
                     KGGDFRSREANVMRLAQLSENIIDQAVAQGVPFNREYSGALATRSFGGVLVQRTFYTR
                     GQTGQQLLLGAYSALEHQVKAGNVEVFNRHEMQDVVVIDGEARGVIVRNLITGELERH
                     AADAVVLASGGYTNVYYLSTNAMGSNVTATWRAHKRGAYFANPCYTQIHPTCIPQSGE
                     YQSKLTLMSESLRNDGRIWVPRKSGDDRPPAEIPEAERYYYLEERYPAFGNLAPRDIA
                     SRAAKVVCDDGLGVGGTGRGVFLDFRDAIAEQGKGEIERKYGNLFEMYTRITGDDPYE
                     TPMMIYPAPHYAMGGLWVDYELQTTIPGLFAIGEANFSDHGANRLGASALMQCLADGY
                     FISPHTVTNHLAKKHDEVSTSDSAFDEVESDVRSHIQELMDVGGSTSAQAFHREMGHV
                     MLDKCGMSRTAEGLDEALTEVRSIKEEFWRDLRVDPGDEEFNQSLEYANRVSDFFELG
                     ELMIHDARRREESCGGHFREEHQTDDGEALRDDDHFAKVTAWEYGGDDPVPHDEALNF
                     EDIKLATRSYK"
     gene            complement(4782..5510)
                     /locus_tag="P5138_00030"
     CDS             complement(4782..5510)
                     /locus_tag="P5138_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRV58992.1"
                     /translation="MIDFWRSSVGKKNIVAVTGAILIGYLILHMLGNLNSAFGPGANE
                     PRVDEYAHWLRDFGEPLLPYAFIVWVVRVVLLSALLIHITGIVQLTKRNREARPVNIP
                     AKRIGRSFESTLMLATGSLILAFIIFHILQFTTLTIDVTPLKEGDVYANLYYAFQKWY
                     FVVIYLAALLLIGMHLRHGVWSLFQTLGFDNPNRNPMLRHGATVFVLILIIGFGSIPV
                     LFFTDVLDPPKTTAMLTALSGVAA"
     gene            complement(5634..5888)
                     /locus_tag="P5138_00035"
     CDS             complement(5634..5888)
                     /locus_tag="P5138_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV58993.1"
                     /translation="MSKHKHGKPKPEGEWQEPDVPDFVGFKKGEKVWVHTRGGGQVPA
                     IYVGDGENATFFGGPPLAYVVLEDSKEGTEVELDRITARK"
     gene            complement(5885..6772)
                     /locus_tag="P5138_00040"
     CDS             complement(5885..6772)
                     /locus_tag="P5138_00040"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRV58994.1"
                     /translation="MFRSPKGRLAAALTGTVVAAFLLGSCEAGTRPATIADLIPAPGA
                     AKEDKGPAILDGCRSGTTHTEAWSCVYGDPGSEQTVVLWGDSHAMQYLPPFVRLAQQR
                     GWRLVTMFRGNCLTADTPYKPNCDAWRANAFERIEEEQPDLVVTSTDTGNGYALWQDG
                     ERLTRDASEPLLRAAYARTLERLEGMTGDRPGGVMVIRDLPRASFRPPDCLLENPDDL
                     AACDFRGFRKNAPGFDLAAARQVGGVRLVDLSDIVCPGGICSSAHNDMVVYRDMTHLS
                     ATYAATLTDWFGEQVGSLP"
     gene            complement(6787..7644)
                     /locus_tag="P5138_00045"
     CDS             complement(6787..7644)
                     /locus_tag="P5138_00045"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRV58995.1"
                     /translation="MIKGVLAASLVALFVLSGVAWAEEGPEWIPPLEKASRDRGEMAM
                     DDCRTKPDQIQPVMCVYGDPHADKTVALIGDSHAMQWGPPLIRLAERRGWRLVTFLRM
                     GCPIADVKFEPNCDRWRDLALTQLADQRPGHIIVSTSIARRYTLSVRGRSLSRRGSEK
                     MLRRGMTRTLRRLAGMPSLVRRGQKVKLIRDQITAPFLPPKCLANHPDDPGFCRFARH
                     RKFGPGFDVAGATKLGLLPAIDPLQALCAPRWCYSARGNVVIYRDSDHLTATFARTLT
                     DWLGRRIGF"
     gene            7746..8363
                     /locus_tag="P5138_00050"
     CDS             7746..8363
                     /locus_tag="P5138_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV58996.1"
                     /translation="MSEGFIHVFEPGDPSRTLLLLHGTGGDENDLVSLGRQLAPGAGI
                     LSPRGKVLENGMPRFFRRLEVGKLDIPDLLARTDELADWLGRAAGDYGFDPAGVIGLG
                     ISNGANIAASLLFRRPETLGGAALLRPMLPYPPPGDLDLDGAPVLVASGGQDHFVPAA
                     ESEELAQQLEAHGAEVEYVFDPASGHGITQDDLLAVRAWLATAFG"
     gene            8477..10120
                     /locus_tag="P5138_00055"
     CDS             8477..10120
                     /locus_tag="P5138_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV58997.1"
                     /translation="MPLKKSTFRGKVIRLDKSEWKSLTGKNRKVTCDIRSGAGHPARI
                     QRPGTRLSGPRLPSGSIWFVSLKNQAATYRKRATSGKARESTRKKLRKQANRLNSLAN
                     KLKLTCPVKSDPGSDPDPKPDPDPDPKPDPDPLTFDFRNASALALLSGSARKQKVSLR
                     GASPSESGLIAWGQNQSAFDPVASGELDGSIMLMALAPDNDLIFVTSAALTSNTSCKL
                     YRADFETGDVVCISAQWLTYPDQVRFDDAGNAYFWGTGFGGSDYGTYLKKSTPEGDTS
                     DVIDPAVVANIAKTLPSTSIGEALVPFEVSPNGDVLIVVGEKLRRIKSDGTPEVVWPN
                     DTELCGFHDGKLFVVSQGQIFTYTPGAASLDEPAWIGPPGSGATHSTTEISDGWIFSW
                     TPTCPSAALGDGALIGTQTVSGSDPLLGPTSTLGLVEIYPAVKRLNFEENYSQITSRP
                     SLTVVGSRLVINHRDPDDKLTIDIYDHTSDSAVHLIPPTGDLEVIHMVYSPGDDLIYF
                     DGINYETFQNFLGTIDPDTGEQQYLDSYSGDLGQIFAFN"
     gene            10237..11535
                     /locus_tag="P5138_00060"
     CDS             10237..11535
                     /locus_tag="P5138_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRV58998.1"
                     /translation="MSDEEKQYGPETNALHAGQVADPTTNARAVPIYQTVSYEFNDAQ
                     HAQDLFALAEPGNIYSRIMNPTWNVLEERMAALEGGIAALATASGQAAVTYSVLNVCR
                     TGDNIVALSTLYGGTYNLFAHTLPQYGIEVKFVDPDKPEELERHVDEKTRLVFAETIG
                     NPRLNVVDIEAWANAAHAHGLPLVVDNTVATPVLANIFELGADIAVHSATKFIGGHGT
                     TLGGVIVDSGNFDWVANAERFPGLTKPDPSYHGVVWADAMGPAAYIGRVRTVLLRNTG
                     AALSPFNAFLLLQGLETLPLRMERHSENAMAVARHLSDSQDVLWVNYPGLPGFSHHGT
                     ASKVLTGGFGALVTFGIKGGRDAGRTFIDSLELFSHLANIGDAKSLAIHPATTTHSQL
                     NDEELEDSGVTADTVRLSVGIESIADILGDIDQALAKAQS"
     gene            11540..12703
                     /locus_tag="P5138_00065"
     CDS             11540..12703
                     /locus_tag="P5138_00065"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRV58999.1"
                     /translation="MNGVGTVGTQRAVVFTGDRPLTLASGARLPEVEVAYETYGELNE
                     DRSNAVFICHALSGDAHAAGVHEGDDPDRPGWWDNIIGPGRPLDTDRFFVVCANVLGG
                     CKGTTGPSSVDPETGKPYGLRFPLVQVRDLVEVHRALLRELGVQRLLAAVGGSLGGMQ
                     ALQWAIDHPTEIDGAAVIAASARLSAQNIAFSAVAREAIMRDPDFAEGDYYENDRRPD
                     AGLALARMIGHITYLSEESMREKFGRRIQDAEVPRFGFDVDFQVESYLHYQGQSFVER
                     FDANTYLYMGRTMDYFDPFEDRVAVQKQISQGKTSFLVLSFDSDWRFSTEHARLMASE
                     LRGAGAPVTFQEISSPHGHDSFLFPDPPEYHRTVAGYMDRLAVQRGIPARRAG"
     gene            12700..>12906
                     /locus_tag="P5138_00070"
     CDS             12700..>12906
                     /locus_tag="P5138_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRV59000.1"
                     /translation="MRPDLDIVASLVGEGQRVLDLGCGDGALLENLIDDRGCRGLGVE
                     RELDDFHACVSRGVPVTQGDLEKEL"
BASE COUNT         2302 a         4136 c         4187 g         2281 t
ORIGIN      
        1 attcccaaac cgatttcctt cagcgcctca aaagcgtcct cgtcggtcac atcggccttg
       61 acgaaggtgc caccgatctt gccgaccagc tcgactccct tctcctcgtt caggtccgcg
      121 accatcacct tgtggccggc ctcggccagg cggcgggcgg tcgcctcgcc caatccggag
      181 gcacctccaa agacgatcgc gctttcagat tccatctgtc ctgctcctcg ctgttgttgc
      241 catagagttg ggcgcatgaa acgagcgaac ttcgaacccg aacatcagga cttccgtgag
      301 accgtcggcc gcttcgtcac cgacgagatc atgccgaatt tcgagcagtg ggaggcggac
      361 gccatcgttc cccgcgagct gttccagaag gccggggaga agaacatgct ggcgatgtcg
      421 gtttcggagg agtacggcgg gctcggcctc aaggactttc gcttcaacca gatcgtcggc
      481 gaggagggct actaccaggg cgcgaccggc gcgatgctcg gcatcaccct ccacaacgat
      541 gtctgcctcc cctacttcga ggagtactgc accgacgagc agaaggaacg ctggcttccc
      601 gggatcgtcg acggcagcct gatcaccgcg gtcgcgatga ccgagcccgg gatcggctcc
      661 gatttggcga gtatgtcaac caaggcgatc cgcaacgacg accactacgt cgtcgacggc
      721 tcgaagacct tcatcaccaa cggcatcaac gccgacctga tcatcaccgc ggtcaagacc
      781 gacccgaagg agaagcaccg cggcatgtcg atcatcatca tcgagcgcgg catggagggc
      841 ttcgatcgcg gccgcaacct cgagaaagtg ggcatgcaca gccaggacac cgccgagctg
      901 ttcttcagcg acgtgaaagt cccggtcgag aacctgctcg gcaacgaagg tgacggcttc
      961 aagtacctcg tctcgaacct cgcccaggag cggatgtcga tcgccgtcgc cgcggtcgct
     1021 gccgcacagg ccgcgctgaa ctggaccctc gattacgtca aggagcgcta cgccttcggc
     1081 tcgccgatcg gcaccttcca gaactcccgc ttccagatgg ccgagatcaa gaccgaagtg
     1141 gacatcgcca cggtctacat cgaccgctgc gtcgaggctc tgaacgaggg caccctgacc
     1201 gtcgaggacg cggccgaggc gaagtggtgg agcaccgaga tggccgaccg ggtgatcgac
     1261 aagtgcgtcc agctccacgg tggctacggc tacatgctcg agtacccgat cgcccgcgcc
     1321 tacgccgaca accgggtcac ccgcatttac ggaggcacga ccgagatcat gaaggagatc
     1381 gtcggccgct cgatgggcct ctaggcggta caaaccaccg aggttgaaat ggagagcgct
     1441 atttgacgct cagttcaatc tcgttgaccg agtatccgga tcatgcccgc cgcccgaata
     1501 gcgaagatcg tctccggcgg gcagaccggg gtcgaccggg cggcactcga cgcggggatc
     1561 gccatcggca tcccgtatgg aggctggtgc ccgaagggcg gcctggccga ggacatgccc
     1621 gatcccccgg gcctgctcga gccctatccg gagctgactg aaacgccgtc ggacgacgtc
     1681 agccaacgca ccgaatggaa tgtgcgggac tcggacgcga ccctgatcat caggcccgaa
     1741 ggttcaaagt caccggggac ggacctgacg atcgaactgg cagcacggat gaaccgtcca
     1801 tgccgaaccg tcgacccgga atcgaacgat gccgttgccg aagtcctcgc tttcatcgag
     1861 tcgatcgggc cagaggcagt cctcaacgtg gccggtcccc gggaaagcgg ggcacccgga
     1921 atctacgagg ccgcccggga cctgctcacc cgatcgctat cgaactgacc gggtttccgg
     1981 agaactgcat gcggatgccg gccgcggccg agaaacaaac gtaccttccg gtaagacctg
     2041 ctgagctggc gaggttgagg aagtggttcg caggggacca caaactcaat ctcgaggcct
     2101 actgggtggt cattgccttc ttgggagtgg gcttcttctt gaccttggcc ttgcggtact
     2161 cgcggttgag cattccgatc acgtcgatcg agatctcctg ggggcaaacc cgggagcact
     2221 ctccgtaatt ggtgcagccg ccgaagccct cggcgtccat ctgctcgacc attccgatca
     2281 cgcgggactc gcgttccggc tggccctggg gcaggacgtt gagatgggtg actttggcgc
     2341 cggtgaagag catcgccgcg ccgttcgggc aggccgccac gcaggcgccg cagccgatgc
     2401 agatcgccgc gtccatcgcc ttctcctggg tctccgggga gaccggcatc gagttcggct
     2461 caggctgggg gcctgtggtg gtcgagatgt agccgccggc ctggatcacc cggtcgagtg
     2521 caccgcggtc taccgcgagg tcgcggatca ccgggaaggt gttggcccgg aacggctcga
     2581 ccttgatcgt ctggccgtcg atgaagtcgc gcatgtaggt ctggcaggaa gtgacctgct
     2641 ggggaccgtg cggggtgccg tcgatgacca gcgagcaggc gccgcagatg ccttcacggc
     2701 aatcggagtc gaaggcgatc gtgcgctttt tctcgtggct gagccgctcg ttgagctggt
     2761 cgagcatctc gaggaaggac gcgtcgggat tgatgtcggt gaccttgaag gtctcgaagc
     2821 ggccctggtc ggtttcgtcg gcaccgtcga actgacgcca gatctcaagg gtgaaattca
     2881 cttgtagctc ctcgtcgcca gcttgatgtc ctcgaagttc agggcttcat cgtggggaac
     2941 cggatcatcg ccaccgtact cccaggcggt gaccttggcg aagtggtcgt cgtcgcggag
     3001 cgcctcgccg tcatcggtct ggtgttcctc gcggaagtgg ccgccgcatg actcctcgcg
     3061 gcggcgggcg tcgtggatca tcagctcgcc cagttcgaaa aagtcggaga cccggttcgc
     3121 gtattcgagc gactggttga attcctcgtc gcccggatcg acccgcaggt cacgccagaa
     3181 ctcctccttg atcgagcgca cctcggtcag tgcctcgtcg agaccctctg cggtgcggga
     3241 catgccgcac ttgtcgagca tcacgtggcc catctcgcgg tggaaggcct gggcggaagt
     3301 gctgccgccc acgtccatca gctcctggat gtgcgagcgg acatccgact cgacctcatc
     3361 gaaggcggag tccgaggtgc tgacctcgtc gtgcttcttc gccaggtggt tggtgaccgt
     3421 atgcggcgag atgaagtagc cgtcggccag gcactgcatg agtgccgagg cgccgagacg
     3481 gttggcgccg tggtcggaga agttcgcctc gccgatcgcg aacaggcccg ggattgtggt
     3541 ctggagctcg taatcgaccc agagcccgcc catcgcgtaa tgcggggccg ggtaaatcat
     3601 catcggcgtc tcgtaagggt cgtcgccggt gatccgggtg tacatctcga agaggttccc
     3661 gtacttgcgc tcgatttccc ccttgccctg ctcggcgatc gcgtcgcgga agtcgaggaa
     3721 gacgccgcgg ccggtgccgc cgaccccgag cccgtcgtcg cagacgacct tggcggcacg
     3781 ggaggcaatg tcacgagggg cgaggttgcc gaaggcgggg tagcgctcct ccaggtagta
     3841 gtagcgctcg gcctcgggga tctcggccgg cgggcggtcg tcaccggact tgcggggcac
     3901 ccagatccgt ccgtcgtttc gaagcgactc gctcatcagg gtcagcttcg actggtactc
     3961 gccggactgc gggatgcagg tcgggtggat ctgcgtgtag caggggttgg cgaagtacgc
     4021 gccccgtttg tgggcccgcc aggtcgcggt cacgttcgag cccatcgcgt tggtcgagag
     4081 gtaatagacg ttggtgtagc cacccgaggc gagtacgacg gcgtcggcgg cgtggcgttc
     4141 gagctcgccg gtgatcaggt tgcggacgat caccccgcgg gcctcgccgt cgatgaccac
     4201 cacgtcctgc atctcgtggc ggttgaagac ctcgacgttg ccggccttga cctgatgctc
     4261 gagagccgag taggcgccga gcaggagctg ctggccggtc tggccgcggg tgtagaaggt
     4321 gcgctggacc aggacaccgc cgaaggagcg ggtcgccagc gcgccgctgt actcgcggtt
     4381 gaagggaact ccctgggcga ccgcctggtc aatgatgttc tccgagagct gggcgagccg
     4441 catcacgttc gcctcgcggg agcggaaatc accgcccttg atcgtgtcgt agaagagccg
     4501 gtgggcgctg tcgccctcgt tggcgtaatc cttggccgcg ttgatgccgc cctgggcggc
     4561 gatcgagtgg gcgcggcgcg gcgagtcctg gaagcagaag ctcttgacct tgaagccctg
     4621 ggcaccaagg ctggcggcgc aggaggagcc ggcgaggccg gtgccgatca cgatcacgtt
     4681 cttgccggcc cggttgcggg ggccgaccag ggtgtgggcg tccagccagt tttgccactt
     4741 gtcggcgagc ggcccggcgg ggatgttcga atcgaagacg gtcatgcggc gactcctgaa
     4801 agcgcggtga gcatcgccgt cgtcttcggc gggtcgagga catcggtgaa gaacaggacc
     4861 gggatcgagc cgaagccgat gatcaggatc aggacgaaga cggtggcgcc gtgacgcagc
     4921 atcgggttcc ggttcggatt gtcgaaaccg agggtctgga agaggctcca gacgccgtgg
     4981 cggagatgca tcccgatcag gagcagcgcc gccaggtaga tcactacgaa gtaccacttc
     5041 tggaaggcgt agtagaggtt ggcgtagacg tcgccttcct tcagcggcgt gacatcgatg
     5101 gtcagcgtcg tgaactggag gatgtggaag atgatgaagg cgaggatcag ggagccggtg
     5161 gcgagcatca gggtcgactc gaagctgcgg ccgatccgct tggccgggat gttcaccggc
     5221 cgggcctcgc ggttgcgctt ggtcagctgg acgattccgg tgatgtggat gagcagggca
     5281 ctgagcagga cgaccctgac cacccagacg atgaaggcgt agggcagcag cggttcgccg
     5341 aagtcccgga gccagtgggc gtactcgtcg acccggggtt cgttcgctcc cggcccgaaa
     5401 gccgagttga ggttgccgag catgtggagg atcagatagc cgatcaggat cgccccggtg
     5461 acggcgacga tgttcttctt gccgacgctg gatcgccaga agtcgatcat cggctgattt
     5521 caggctggac ggacttgatc acggaagccg attctattgc ccggcggcga ccgagggctt
     5581 gccgcatctg gctttgggaa gcctaattcg caccccacag ggcgggataa ggcctacttc
     5641 cgtgcggtga tgcgatcgag ctccacttcg gtgccctctt tgctgtcttc cagcaccacg
     5701 tatgcgagcg gggggccgcc gaagaaggtt gcgttctcgc cgtcgccgac gtatatcgcg
     5761 ggaacctggc cgcctccccg ggtgtggacc cagaccttct cgcccttctt gaaaccgacg
     5821 aaatcgggga cgtccggctc ctgccattcg ccttcgggct tcggctttcc gtgcttgtgc
     5881 ttgctcatgg cagggagcct acttgctctc cgaaccagtc ggtgagagtc gctgcatagg
     5941 tggcgctgag gtgagtcatg tcgcggtaga cgaccatgtc attgtgggcg gagctgcata
     6001 tccctccggg gcagacgatg tccgagaggt cgaccagcct gacgccgccg acctgacggg
     6061 cggcggcaag gtcgaagccc ggggcgttct tgcggaagcc ccggaagtca caggcggcga
     6121 gatcgtccgg gttctcgagc aggcagtccg gcggacggaa ggaggcccgg ggcaggtcgc
     6181 gtatgaccat cacccctccc ggtcggtcac cggtcatccc ctccagccgt tcaagggtgc
     6241 gggcataggc ggcccggagc agcggttcgc tcgcgtccct ggtcagcctt tccccgtcct
     6301 gccagagcgc atagccattc ccggtgtcgg tagacgtgac gaccaggtcg ggctgctctt
     6361 cctcgatccg ttcgaaggca ttggcccgcc aggcgtcgca attcggtttg tagggagtgt
     6421 cggcggtcag gcagttgccc cggaacatgg tcacgaggcg ccagccccgc tgctgggcca
     6481 ggcggacgaa gggtggcaag tactgcatcg catgcgaatc tccccagagg accacggtct
     6541 gttccgaccc cggatccccg tacacgcagc tccaggcttc ggtgtgagtg gtacccgaac
     6601 ggcatccgtc gaggatcgcc ggacccttgt cctccttggc cgcaccgggg gccggaatca
     6661 ggtccgcgat ggtcgcggga cgagtgcccg cttcgcagct tccgagcagg aaggcggcaa
     6721 cgaccgtccc tgtcagggcg gcagccagac gccctttggg ggaccggaac atccgggaac
     6781 cgtacttcag aacccgattc ttcttcccag ccagtcggtg agtgtacgcg cgaatgtcgc
     6841 ggtcagatgg tcggaatcgc gatagatcac gacgtttccg cgggccgagt aacaccagcg
     6901 gggagcacaa agcgcctgca gcggatcgat cgccggcaga agacccagct tcgtagctcc
     6961 ggccacgtcg aacccgggac caaacttgcg gtggcgggcg aaacggcaga acccggggtc
     7021 atccgggtgg ttcgccaggc atttcggcgg caggaagggc gcagtgatct ggtcccggat
     7081 cagcttgacc ttctgcccgc gccggaccag gctgggcatg ccggccaggc gacgcagcgt
     7141 ccgggtcatc ccgcggcgca gcatcttttc gctgccccga cgggagaggc tccggccacg
     7201 gaccgagagc gtgtagcggc gggcgatcga ggtcgagacg atgatgtgcc cgggtcgctg
     7261 gtccgccagt tgcgtcaggg ccagatcgcg ccagcggtca cagttcggtt cgaacttgac
     7321 gtcggcgatc gggcagccca ttcgcaggaa ggtgaccagg cgccatcccc ttctctccgc
     7381 gaggcggatc aatggcgggc cccattgcat cgcgtgggaa tcgccgatca gggcgacggt
     7441 cttgtccgcg tggggatcgc catagacgca catgacgggt tggatctgat ccggcttcgt
     7501 ccggcaatcg tccatcgcca tctccccgcg atcgcgagag gccttctcga gcggcggtat
     7561 ccactccggt ccctcctccg cccacgcgac ccccgacagg acgaaaagtg cgacgagcga
     7621 cgcggccaat actcccttga tcatcttgat ccccctttgg actggttggt gacgctgacc
     7681 catgaacacg gaggcggaga atttgttgct gccagatcgg attctgcttc ccctagagtg
     7741 gagccatgtc cgaaggcttc atccacgtct tcgaacccgg cgacccgtcc cggacccttc
     7801 tgctgctcca cggcaccggc ggggacgaga acgacctggt ctcgctcggc cgccagctgg
     7861 cacccggagc cgggattctc agtccgcgtg gcaaggtgct ggagaacggg atgccccggt
     7921 tcttccgtcg tctggaggtc ggcaagctcg acatcccgga cctgcttgcc cgcaccgacg
     7981 aactggccga ctggctggga agggctgccg gcgactacgg cttcgatccg gccggggtga
     8041 tcggcctcgg gatttccaac ggcgccaaca tcgccgccag cctgctcttc cgtcgacccg
     8101 aaaccctggg gggcgcggcg ctgctcaggc cgatgctccc ctacccgccg ccgggggacc
     8161 tggacctcga cggcgctccc gtcctggtcg cctcgggcgg gcaggaccat ttcgtgcccg
     8221 cggccgaaag tgaagagctt gctcagcagc tggaggctca cggagcggag gtcgagtatg
     8281 tcttcgaccc cgcctcgggc cacgggatca cccaggacga cctgctcgcg gtgcgagcct
     8341 ggctggcaac tgctttcggt tgaaggaccg atcacataag atccggcgca tgaaaagagt
     8401 cggaggccgg attgcctcga tcctgggagt tgttgtcatc gcttgtgcat gtgcaagcgc
     8461 gtctactgct gctgccttgc cactgaagaa gtcaactttc aggggaaagg tgatccgact
     8521 cgacaagtcc gaatggaaat cactgaccgg caagaaccgg aaagtgacct gcgatatccg
     8581 atccggtgct ggtcacccgg cacgcataca acggccggga acgagacttt cgggtccgcg
     8641 gctccccagt ggttcgatct ggttcgtttc cctcaagaac caggcggcga cctaccggaa
     8701 acgagcgaca tcgggcaagg cccgcgagtc gacccggaag aaactgcgaa aacaggccaa
     8761 tcgtctgaat tcgctcgcca acaaattgaa actcacttgt ccggtcaagt cagacccggg
     8821 gtctgatccg gacccgaagc cggacccgga cccggacccg aagccggacc cggaccctct
     8881 cacgtttgac tttcgcaatg cctcggcgct ggccttgcta tcagggtcgg cccgaaagca
     8941 gaaggtgtcc cttcgtggcg cttcccccag cgaatcagga ttgatcgcct ggggacagaa
     9001 ccagagcgca ttcgatccgg tcgcatcggg tgagctggac ggttcgatca tgctcatggc
     9061 tttggcgccg gacaacgacc tgatcttcgt tacatcggcc gccttgacat cgaacacgtc
     9121 ctgcaagctg taccgggcag attttgagac cggcgatgtc gtttgcatat cggctcaatg
     9181 gctgacctat ccggaccagg tcaggttcga cgacgcgggc aacgcgtatt tctggggcac
     9241 cggattcggt ggctcggact acggcaccta cctcaagaaa tcgactcctg aaggtgacac
     9301 ctccgatgtg atcgaccctg ccgtcgttgc gaacatcgcc aagactttgc cctccacttc
     9361 aattggcgaa gccctcgtgc cgtttgaagt ttcaccgaac ggagacgtgc tgatcgtcgt
     9421 gggcgaaaag ctacgtcgaa tcaagtccga tggaactccg gaagtggtct ggccgaacga
     9481 caccgaactt tgtggatttc atgacgggaa actcttcgtg gtgtcgcaag ggcagatatt
     9541 cacttacaca cccggagcgg catctctgga tgagcctgcc tggatcggtc cgccagggtc
     9601 tggagccacc cacagcacca cggaaatatc ggacgggtgg attttctcct ggaccccgac
     9661 ctgtccgtcg gccgcactcg gggatggcgc gttgatcggc acgcaaaccg tttcaggaag
     9721 cgaccctctg ctgggcccca ccagcacact tgggctagtc gagatctacc ccgccgtgaa
     9781 gcggctcaac ttcgaagaga actactcgca gatcacatcc cgacccagct tgaccgtggt
     9841 cggatcaagg ctggtcatca accacaggga tcctgacgac aaactgacca tcgacatcta
     9901 tgaccacact tcagattcgg cagttcatct cataccgccg accggtgatc ttgaggtgat
     9961 tcacatggtc tattcaccgg gtgatgacct gatctacttc gatggcatca actacgagac
    10021 tttccagaac tttctcggaa cgatcgatcc ggacaccggt gaacagcagt acctggattc
    10081 ctattcggga gatctgggtc agattttcgc tttcaactga ttgccgacac cgggcgtctg
    10141 aaccccggct ccccgggtgc tgtgtttctc atctgagcta ctaggctttc cggtcgcgat
    10201 agcgtcagag gaccacgcca cccaatcagg agagaaatga gcgacgaaga gaagcagtac
    10261 ggcccggaga cgaacgccct gcatgcgggc caggtggccg acccgaccac caacgcccgg
    10321 gcggtgccga tctaccagac ggtctcttac gaattcaacg acgcccagca tgcccaggac
    10381 ctgttcgccc tggccgagcc gggcaacatc tactcgcgga tcatgaatcc gacctggaac
    10441 gtgctcgagg agcggatggc tgccctcgag ggcgggatcg ccgccctggc caccgcctcg
    10501 gggcaggccg cggtcaccta ttcggtgctg aacgtctgcc ggaccggcga caacatcgtc
    10561 gccctctcga ccctgtacgg cggcacctac aacctgttcg cccacacgct cccgcaatac
    10621 gggatcgagg tgaagttcgt cgatccggac aagccggaag agctcgagcg ccacgtcgac
    10681 gagaagaccc gcctggtctt cgcggagacg atcggcaacc cgcgcctgaa cgttgtcgac
    10741 atcgaagcct gggccaatgc cgcgcacgcc catggcctgc cgctggtggt cgacaacacg
    10801 gtcgcgaccc cggtgctggc caatatcttc gagctcggcg ccgacatcgc ggtccattcc
    10861 gcgaccaagt tcatcggtgg tcacggcacc accctcggcg gcgtgatcgt tgactcgggc
    10921 aacttcgact gggtggccaa cgccgagcgt ttcccgggac tgaccaagcc cgacccctcc
    10981 taccacggag tggtctgggc ggatgcgatg ggccccgccg cctacatcgg ccgggtccgg
    11041 acggtgctgc tccgcaatac cggcgcggcg ctttcgccgt tcaatgcatt ccttctgctg
    11101 cagggcctcg aaaccctgcc gctccggatg gagcgccaca gcgagaacgc gatggcggtc
    11161 gcccggcacc tgagcgacag ccaggatgtg ctctgggtca actacccggg cctgcccggc
    11221 ttcagccacc acggaacggc gagcaaggtc ctgaccggcg gcttcggtgc cctggtcacc
    11281 ttcgggatca agggcggccg cgacgcgggc cgcacgttca tcgactcact ggagctgttc
    11341 tcgcacctcg ccaacatcgg cgacgcgaag tcgctggcaa tccacccggc gaccaccacc
    11401 cactcgcagc tcaacgacga ggagctcgag gattccgggg tcaccgccga cacggtgcgc
    11461 ctctcggttg gcatcgagtc gatcgccgac atcctcggcg acatcgacca ggcgctggca
    11521 aaggcccagt cgtagctagt tgaacggggt cggcactgtc gggacgcaga gggcggtcgt
    11581 cttcaccggc gaccgcccgc tgacccttgc ctccggggcg aggctccccg aagtcgaggt
    11641 cgcctacgag acctacggcg agctcaacga ggaccgctcc aacgcggtct tcatctgcca
    11701 cgcactttcc ggtgatgccc acgcggccgg agtccacgaa ggcgacgatc ctgaccgccc
    11761 cggctggtgg gacaacatca tcggtccggg acggccgctg gacaccgacc gcttcttcgt
    11821 ggtctgcgcg aacgtgctcg gcggctgcaa gggcaccacc ggcccctcat cggtcgaccc
    11881 ggagacgggc aagccgtacg gcctccgctt cccgctggtc caggtccgtg acctggtcga
    11941 ggtgcaccgg gccctgctcc gcgaactcgg ggtccagcgg ctgctggccg cggtaggagg
    12001 ctcgctcggc ggcatgcagg cgctccagtg ggcgatcgac catccgaccg aaatcgacgg
    12061 cgccgcggtg atcgccgcct cggcgcggct gtcggcccag aacatcgctt tctccgcggt
    12121 cgcccgcgag gcgatcatgc gtgatccgga cttcgccgaa ggcgactact acgaaaacga
    12181 ccgccgcccg gacgcgggcc ttgcgctcgc ccgcatgatc ggccacatca cctacctctc
    12241 ggaagagtcg atgcgcgaga agttcggtcg ccggatccag gacgccgagg tgccccgatt
    12301 cggcttcgac gtggacttcc aggtcgagag ctatctccat taccagggcc agtccttcgt
    12361 cgagcgcttc gacgcgaaca cctacctgta catgggccgg accatggatt acttcgatcc
    12421 tttcgaggac cgggtcgcgg tccagaagca gatcagccag gggaaaacca gcttcctggt
    12481 gctctccttc gactccgact ggcgcttctc caccgaacat gcccgactga tggccagcga
    12541 gctgcggggg gcgggggcgc cggtcacctt ccaggagatc tcctccccgc atggacatga
    12601 ctccttcctc ttccccgacc cgcccgagta ccaccggacg gtcgccggct acatggaccg
    12661 gctcgccgtc cagcgcggca tccccgcgag gagagcaggt tgagaccgga cctcgacatc
    12721 gtcgcttcgc tggtcggcga aggccagcgg gtgctggacc tcggctgcgg tgacggggcg
    12781 ctgctcgaga atttgatcga tgaccggggc tgccgcggcc tcggggtcga acgggaactc
    12841 gacgacttcc acgcctgcgt ttcccggggc gtgccggtca cccagggtga cctcgagaag
    12901 gaactg
//
LOCUS       DAOTIP010000002        11112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_6985_length_11112_cov_2.956769, whole genome shotgun sequence.
ACCESSION   DAOTIP010000002 DAOTIP010000000
VERSION     DAOTIP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6985_length_11112_cov_2.956769"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1282
                     /locus_tag="P5138_00075"
     CDS             185..1282
                     /locus_tag="P5138_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HRV59001.1"
                     /translation="MSSIETFTLRDVGPKGGLEQAEMVAGRIVDFVGRAERSLVLALY
                     DIRLPDPVGSVVADAFRTAAERGVKIRLAYNDVPEDPDAGNFLPAVHPPPETNPEILG
                     KLPLETKPISGIPDLMHHKYMVRDGKAVWTGSANWSIYSWTRQENVLAVIEHGEVARE
                     YLENFEELWQRGKVERTGRGDPNPIRVGEATVRAWFTPGHGEELAQRISTRLGAARRR
                     IRIASPVLTSGPILGTLAEIATRPGLDIAGVVDEPQTDRVYEQWATTGSKWKIPLLTR
                     VIESLPFSGKRSVPWGGGTTRDFMHAKVTVADDTVFLGSFNLSRSGEENAENMVEIED
                     PAVADAMAAFIDETRARYPRSSVPGSRVAGR"
     gene            complement(1262..2347)
                     /locus_tag="P5138_00080"
     CDS             complement(1262..2347)
                     /locus_tag="P5138_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRV59002.1"
                     /translation="MGISIPAVSSPDDRHHEPEGEVWVGVRIEGTESPLRAEAIRSAL
                     ARAGAPFVEPTVYPDSSITAVHDQALVDWQATAHDEWVRAGLDRDPGQDRVVPYIFPH
                     PGLLEGIDPIEPSSASARAGRYVYDTMTLIAPGTWKAARAAADVALTAAGLVESGTQA
                     TYGLCRPPGHHAARSAIGGSCYLNNSAIAAGSLAGTGRVGVLDIDAHHGNGTQSIFYE
                     DPGVLTCSVHVDPREGWFPHFFGLAGETGRGPGEGTNRNLPLPAGTGDREWLAGVEGL
                     LDWLLAGGAGKLVVALGLDAAAGDPEGPFEVSEAGYRSAGRLIGAAGLPTVFIQEGGY
                     ALDTLGDLVIAALEGFEEGAASATGDA"
     gene            2447..3469
                     /locus_tag="P5138_00085"
     CDS             2447..3469
                     /locus_tag="P5138_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRV59003.1"
                     /translation="MSEAYGTGRVIDPAGALPQPADRLDPSGPVREDEFEIAVERLCL
                     DATSFRSIRREAGADPDRMADRVMEIVTDRGKMHNPETDSGGVALGTVTGVGAGYDDP
                     PSEGDRIVTLASLTLTPLRIDEITGLDPDSAQIAVRGTAYVFNSAPWGPLPSDLPEET
                     ALEVYDVYGAGSHVARLAGPGDTVCVMGAGHAGKLAMAAAREVTDGGTVIGIDADPQA
                     IGRLDELGLCDIGLVCDLRDPISTRDRLVEAGAPEADLTVVVVSSTGCEPAAIIATAE
                     GGTVLFFSMATSFQTAALTADGIGRDVRMLVGSGYTPDCGSWSLDLLRRTAPLRAAFG
                     LAEATS"
     gene            3466..3849
                     /locus_tag="P5138_00090"
     CDS             3466..3849
                     /locus_tag="P5138_00090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRV59004.1"
                     /translation="MSFEVQTRWQDIDGLGHVNHAAVLNFFEEGRDAFLGKCGLGPVD
                     YVVGRCSVTYLREIKPENRSVSVSCTVERIGRSSFQTSEQIFDGDGRLAVEASFGLVM
                     WDQTKSSPRPITDEERSAMNQQGGE"
     gene            3850..4677
                     /locus_tag="P5138_00095"
     CDS             3850..4677
                     /locus_tag="P5138_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRV59005.1"
                     /translation="MAWDSDPVVLTVAVTGADVFRENNPNIPYTTAEIADSSIEAAEA
                     GATVVHLHVREEDGTPSGRPELFQDVIGRIREGSDLLTMVSTGGSNDMSIEERTTGLE
                     AAPDISGVESGSMNFGDETFITAPPAGRGIVKRALDAGIALEVECFEVGHVVSAVKWL
                     EDGLLPAPLRINLVFGVPGGIDASPEALDAMLRPLPEGTFWTVTCIGRHHRRMIALAL
                     LKGAPGIRTGLEDVSYLSRGVLAPSNASLVEMAVDMARSLGREVAGQAETRELLSLG"
     gene            4670..5314
                     /locus_tag="P5138_00100"
     CDS             4670..5314
                     /locus_tag="P5138_00100"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV59006.1"
                     /translation="MAETKTTGARRGEATREAIEAAAVDLFAERGYHATSMREIASAA
                     KVQPAAIYHWFDSKEKILTRLQDDFMDRLEAAVEEAVAAEATPPGQLAAAVREHVVFH
                     GLHAREAFVTDSEIRALSTRPRNRLIARRDAYQTMFRQMIEAGVKSGDFTISNPAVAS
                     YAILLECTGVALWYDPKGALALDEIAAIHVELVLGSLGAAPKVAGRTGARKERR"
     gene            5311..6270
                     /locus_tag="P5138_00105"
     CDS             5311..6270
                     /locus_tag="P5138_00105"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRV59007.1"
                     /translation="MNRIKPKALPEKATIAVVAPASPPQTRSEIEQATEYFESRGHEV
                     VFGPNHRNVHGYLAGTDEERAADLQWALTEPGIDMVHALCGGYGSARLYERIDWDNLG
                     EPRIVCGFSDITGLHLALARKPGWVTFYGPNFLRFTRRKDELTKETEDWFHRAFQAEP
                     LGEVFEDPEDPFVLGVGGGIAEGTLVGGCLTLVSASIGTPFEVETDGCVLMVEDLNTD
                     QYLIDTLLNHLLRAGKLDNIAGFVFGTDVMLQSRTPPEGPESTLSIEEVLDELIAPLG
                     IPAIANVPVGHGKHMATMPLGAHVRLDGDRKKLEVTEPAVEPA"
     gene            6295..8154
                     /locus_tag="P5138_00110"
     CDS             6295..8154
                     /locus_tag="P5138_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV59008.1"
                     /translation="MNSKRIRNIAAAVIAALALLGAMALPAAADEQGDAPVLSIGWAQ
                     DPQTLNPFIGLDEEDYTIWAINWDLLVNFDPEDLSPAPGIAESWKVSDDKKTVTFKIA
                     DRKWSDGVPVTSADVKWSLETLGSEGELFTSYTNNVTSITTPDKKTVVIKTSKPDARI
                     VGGLLIYILPKHIWGKVPLSELTTTYQPSLPLVGTGPFIVTEFDRGRLIKMDQNPEFD
                     GKKPKFGQLQFIKYGNQDAVERALQLGEIDLVPEVQASTFKRLGEQDNIKTVDSPSPA
                     FTELAFNLCPEKLCPDAQVNPAIRDLPVRQAIAYSIDRERINQIAASDTSFVANGLLP
                     SYYKSFYEDPENTYPFDPEKAKQILDDAGYLPGDDGIREKDGDRLSFNLYTRTRSESA
                     YNAQAAKLVAEMARDVGIEFKVQEVSVDKLTELTVRQVDGKPAPDFDAFIWGWGGDAY
                     DPSFLLSLMTTGEIGGSSDSFYSNAEYDKLFNEQSGEFDVEKRKEIIHRMINRLQQDL
                     PYIVLTEDPNLQAYRTDRIANVEPTCPSGDGDIFCEQAGYAPLLDLAPGSSSGDEGSS
                     GNGVLIAIIAALVIAVLAFVLIRHRRRRDEPLEVEEETLEVEEQPDGTDGEEK"
     gene            8162..9130
                     /locus_tag="P5138_00115"
     CDS             8162..9130
                     /locus_tag="P5138_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV59009.1"
                     /translation="MSVRWLSGKVAAAALTLLFVIVFNFFLFRVMGDPTDQLARLPRA
                     TPEEIQKLRGQYGLDKPMLGQFTDYMGDTARLDFGISQRSRRTVTDEIKDALPWTLLL
                     VGTGTLLATLLGSWMGVKAATRRGKHSDQALLGFNLFTYASPEYWIGIILILVFAVWI
                     PILPSGLQVTPGTEFSNFFSQALDVAKHLILPATAMTLMLIGQYFLIMRSSMVDVLTE
                     DFITVKRATGMRWDRVVNRHAVPNALLPLVTLSAIQFGAVVAGAITIETIFSWPGLGE
                     LTYQAINDKDFPVLQGTFLFFSFAVILANLAADCLYFFLDPRVQGR"
     gene            9127..9978
                     /locus_tag="P5138_00120"
     CDS             9127..9978
                     /locus_tag="P5138_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV59010.1"
                     /translation="MSGREAVRVLFSSWRGRFGIAVLVVTVFVAIFGSALAPYDPTAS
                     SLDVLQPPSFDHLLGTTESGEDVFSQLLVGTRTSVVVGFAAAVISAVLGSAVGIFSGY
                     FGGWTDRILDSLENWFLVIPTLPLMIVLARLLDPSLGVLVAVIGLTSWAGTGRIVRAQ
                     VMTLRERAFVERARALGAGDTYIIRTHVLPNTLPLIFANTVLIIAVAILSEAALSFLG
                     LGDPSRISWGSMLENAFGSGAPSAGAWWYVIPPGLCITILVLAVTLIGFLYEEYINPR
                     LAMDRES"
     gene            9975..10976
                     /locus_tag="P5138_00125"
     CDS             9975..10976
                     /locus_tag="P5138_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV59011.1"
                     /translation="MSSILKIRDLHVAYQGKEIVEGVDLDLARGESLGLAGESGCGKT
                     TTALAVMKLLPEGFTQSGSITLSPPGEAEPIRIDRRTEAGMRVVRWRHVSLVFQGAMN
                     SLDPVKRVGPQIEEVIVLHEGRSGRISGRIDELLGTVGLTPAIARRYPHQLSGGQKQR
                     VMIALALACGPSVVIADEPTTALDVVMQAQVLQLLEKLREELGLALVLISHDLGVMAE
                     TCDRIAIMYAGRIVETGPTDQVLADPQHPYTRRLLESLPVIGGERGLAPPIPGIPPAP
                     GDVVEGCAFRPRCPYSEDRCLQDPALRQIGDLRSAACHFSPWSSWPEVAATGKETAR"
BASE COUNT         1917 a         3682 c         3692 g         1821 t
ORIGIN      
        1 tcgacgacgt cctgatcacg gtcggcggca cgatcccgaa caaggacatc gaggcgctga
       61 aggagatggg cgtctccggg gtcttcacgc ccggcgcccc gaccgacgaa atcgtcgagt
      121 tcatccgggg cggagtcaag cgccgcgacc tttagtcccg gccaggcgcg ggtagggttc
      181 gcgcatgtcc tcgatcgaga ctttcaccct ccgggacgtc gggcccaagg gcggactcga
      241 gcaggccgaa atggtggccg ggcggatcgt cgacttcgtc ggcagggcag agcggtcgct
      301 ggtcctggcg ctctatgaca tccgcctgcc cgacccggtc gggtctgtgg tcgccgacgc
      361 cttccggact gcggccgagc gcggggtgaa gatccgtctc gcctacaacg acgtgcccga
      421 ggaccccgac gccggcaact tccttccggc cgtccatccg cccccggaaa cgaatccgga
      481 gatcctcggg aagcttccgc tcgaaacgaa gccgatttcc gggataccgg acctgatgca
      541 tcacaagtac atggttcggg acgggaaagc ggtctggacc gggtcggcca actggtcgat
      601 ctactcctgg accaggcagg agaacgtgct ggccgtcatc gaacacggcg aggtcgcccg
      661 cgagtacctg gagaacttcg aggagctctg gcaacgggga aaggtggagc ggaccggtcg
      721 cggtgacccg aacccgatca gggtggggga ggcgacggtg cgggcctggt tcacccccgg
      781 ccacggcgag gaactggccc agcgtatttc gaccaggctc ggtgcggccc gacgacggat
      841 caggatcgcc tcaccggtcc tcacctcggg gccgatcctc ggcaccctgg ccgaaatcgc
      901 aacacggccc gggctggaca tcgccggggt ggtcgacgag ccccagaccg accgggtcta
      961 tgaacagtgg gcgacgaccg gctcgaaatg gaagatcccg ctgctcaccc gggtgatcga
     1021 gagcctgccc ttcagcggca agcggtcggt gccctggggc ggcggtacca ctcgcgattt
     1081 catgcacgcg aaggtgacgg tggccgacga cacggtcttt ctcggttcct tcaacctttc
     1141 ccgttcgggg gaggagaacg cggagaacat ggtcgagatc gaggacccgg ccgtcgccga
     1201 cgcgatggcc gccttcatcg acgagacccg ggcccgttac ccgcggagct cggtacccgg
     1261 ctcacgcgtc gccggtcgct gaggctgccc cctcttcgaa gccttcgagg gcggcgatca
     1321 ccaggtcgcc aagagtgtcc agcgcgtagc cgccctcctg gatgaacaca gtcggcagtc
     1381 ccgccgcacc gatcagccgg ccggccgaac ggtagcccgc ctcgctcacc tcgaaggggc
     1441 cttccggatc acccgcggcc gcgtcgagtc cgagcgcgac gacgagcttc ccggcaccac
     1501 ccgcaagcag ccaatccagc agtccctcga cccccgccag ccattcccgg tccccggtgc
     1561 cggctggcaa gggaaggttc cggttcgtcc cttcgccggg tccgcgtccg gtctctccgg
     1621 caagtccgaa gaaatgggga aaccagccct ctctcgggtc gacgtgaacc gaacaggtca
     1681 ggaccccggg gtcctcatag aagatcgact gggtgccgtt gccgtgatgt gcgtcgatgt
     1741 cgaggacacc gacacgaccg gtgccggcaa ggctcccagc cgctatcgcg ctgttgttca
     1801 ggtagcagga accaccgatc gcgctgcggg cagcgtggtg tcccggagga cggcacagcc
     1861 cgtaggtggc ctgggtgccc gattccacga ggccggcggc ggtcagggcc acgtcggcgg
     1921 ccgcacgggc cgccttccag gttccgggag cgatcagggt catggtgtcg tagacgtatc
     1981 tgccggcccg ggcggatgcc gatgacggtt cgatcgggtc gattccttcg agcaggccgg
     2041 gatggggaaa gatgtacggc accacccggt cctggccggg atcacggtcg aggccggccc
     2101 ggacccactc gtcgtgggct gttgcctgcc agtcgaccaa tgcctggtcg tggacggcgg
     2161 tgatcgagga gtcgggatag acggtcggct cgacgaacgg tgccccggcc cgggcgaggg
     2221 cggaccggat tgcctcggct cggagcggcg attccgtccc ctcgatacga accccgaccc
     2281 acacctctcc ttccggctcg tggtgccggt catccggcga gctgacggca ggtatcgaga
     2341 tccccatggc ggagttgtct cagattcgtg cttgacgacg gcggtccatc tcaataacct
     2401 aacggtcgtt catgaagttt ggggccgaag atctggggat catcgattga gtgaggcgta
     2461 cggaaccggg cgggtcatcg accctgccgg cgcgctgccg cagccggccg accggctcga
     2521 cccttcgggg ccggtccgcg aagacgagtt cgagatcgcg gtcgagcgac tttgccttga
     2581 cgccacctcc ttccgcagca tcaggcggga ggccggggcg gacccggaca ggatggccga
     2641 ccgggtgatg gagatcgtca ccgaccgcgg aaaaatgcac aacccggaaa ccgattccgg
     2701 gggagtggcg ctgggaacgg tcaccggggt cggggccggt tacgacgacc cgccatcgga
     2761 aggcgaccgg atcgtcaccc tggcatccct caccctgact cccctgcgga tcgacgagat
     2821 caccgggctc gacccggaca gcgcccagat cgccgtccgg ggcactgcat acgtcttcaa
     2881 ttccgccccc tggggtcccc tgccctccga cctgccggaa gaaaccgcgc tcgaggtcta
     2941 tgacgtctac ggcgccggtt cccatgtggc acggctggcc ggtccggggg acacggtctg
     3001 tgtgatgggc gccggacacg ccgggaagct ggcgatggcg gcggcccggg aagtgactga
     3061 cgggggcacg gtgatcggga tcgatgccga tccgcaggcg atcggccggc tggacgagct
     3121 cggcctttgt gacatcgggc tggtctgcga cctgcgtgat ccgatctcca cccgggacag
     3181 gctggtcgag gccggcgcac cggaagccga cctgaccgtg gtcgtggtca gctcgaccgg
     3241 ctgcgaaccg gcggcgatca tcgccactgc cgagggaggc accgtcctct tcttctcgat
     3301 ggccaccagt ttccagaccg ctgcgcttac ggccgacggc atcggtcggg acgtccggat
     3361 gctggtcggc tccgggtaca cgccggactg cggctcctgg tcgcttgacc tgctccggcg
     3421 cacggcgccg ctcagggcgg cgttcggtct tgcggaggcg acctcgtgag cttcgaggtc
     3481 cagacccgct ggcaggacat cgacggcctc gggcatgtca atcacgccgc ggtgctcaac
     3541 ttcttcgagg aagggcggga cgcctttctc ggcaaatgcg gactgggacc ggtcgactac
     3601 gtggtcggtc gctgctcggt cacctacttg cgggagatca agccggaaaa tcgctcggta
     3661 tccgtttcct gcacggtcga acggatcggc cggtccagct tccagaccag cgagcagata
     3721 ttcgacgggg acggacggct tgcggtcgag gcgagcttcg ggctggtcat gtgggaccag
     3781 acgaaatcga gtccccggcc gataaccgac gaggaacgca gcgcgatgaa ccagcaagga
     3841 ggggagtaga tggcctggga ttcagacccg gtggtgctta ccgtggccgt gaccggggcc
     3901 gacgtgttcc gggagaacaa cccgaacatc ccctacacga cggcggaaat agccgactcg
     3961 agcatcgagg cggccgaggc cggtgccacc gttgtccacc tgcatgtccg ggaagaagac
     4021 ggcaccccga gcggccggcc cgaactcttc caggacgtga tcggccggat ccgggaagga
     4081 tcggacctgt tgaccatggt ctcgaccggc ggcagcaacg acatgtcgat cgaggagcgg
     4141 accaccgggc tggaagccgc cccggacatc tccggggtcg agtcgggttc gatgaacttc
     4201 ggcgacgaga ccttcatcac cgcgccgcca gccggccggg gaatcgtcaa gcgggccctg
     4261 gatgccggta tcgcgctcga ggtcgaatgc ttcgaggtcg gccatgtggt cagcgctgtc
     4321 aagtggctgg aggacggtct gcttccggcc ccgctgcgga tcaacctggt cttcggcgta
     4381 cccggaggta tcgacgcctc gccggaggct ctcgacgcga tgctccggcc gctgccggag
     4441 gggaccttct ggacggtcac ctgtatcggc aggcatcacc gcaggatgat tgccctggca
     4501 ctgctcaagg gcgcacctgg cattcgcacc ggactcgagg atgtctccta tctgagccgt
     4561 ggggtgctgg ctccttcgaa tgcttcgctg gtcgagatgg cggtcgacat ggcccgctcg
     4621 ctgggtcgtg aagttgccgg ccaggcggaa acccgggagc tgctctcgct tggctgagac
     4681 caagacgaca ggcgcgcgcc ggggcgaggc gaccagggag gcgatcgagg ccgctgccgt
     4741 cgacctgttc gccgagcgcg ggtaccacgc aacctcgatg cgcgagatcg ccagcgcggc
     4801 gaaagtccag ccggccgcga tctatcactg gttcgactcc aaggagaaga tcctcacccg
     4861 gcttcaggac gacttcatgg accgactcga agcggcggtc gaagaggcgg tcgcggcgga
     4921 agcaacgccg cccggccagc ttgccgcggc ggtgcgtgaa cacgtggtct tccacggact
     4981 tcacgcccgc gaggccttcg tcaccgacag cgagatacgc gccctgagca cccggccgag
     5041 aaaccggttg atcgcccgcc gcgacgccta ccagacgatg ttccgccaga tgatcgaggc
     5101 cggcgtcaag tccggcgact tcacgatctc gaatcccgca gtggccagct acgcgatcct
     5161 tctcgaatgc accggggtag cgctctggta cgacccgaag ggagcgcttg cgcttgacga
     5221 gatagcggcc atccacgtcg agctcgtgct cggctcgctg ggtgctgcgc cgaaggtggc
     5281 cggcaggacc ggcgccagga aggagagaag gtgaaccgga tcaagccgaa ggcgttgccg
     5341 gagaaggcaa cgatcgccgt ggtggcgccg gcgagcccgc cccagaccag gtccgagatc
     5401 gagcaggcga ccgaatactt cgagtcgcgt ggccacgagg tcgtcttcgg cccgaaccac
     5461 cgcaacgtcc acggctacct ggccgggacc gacgaggaac gggcggcgga tcttcaatgg
     5521 gcgctgaccg agccggggat cgacatggtc cacgccctct gcggcggata cggctcggcc
     5581 cggctctacg aacggatcga ctgggacaac ctcggagaac cgcggatcgt ctgcgggttc
     5641 agtgacatca ccggcctgca cctcgctctc gcccgcaagc ccggctgggt caccttctac
     5701 ggccccaact tcctgcgctt cacacgtcgc aaggacgagc tcacgaagga gaccgaggac
     5761 tggttccatc gggccttcca ggccgagccg ctgggtgagg tcttcgagga tccggaggat
     5821 cccttcgtgc tgggcgtagg cggggggatt gccgagggaa ccctggtcgg cggttgcctg
     5881 accctggtct cggcaagcat cggaaccccc ttcgaggtcg agactgacgg ttgcgtgctg
     5941 atggtcgagg acctgaacac ggaccagtat ctgatcgaca ccctgctcaa ccacttgctg
     6001 cgggccggga agctcgacaa catcgccggt ttcgttttcg gaaccgacgt gatgctgcag
     6061 tcccggaccc cgcccgaagg acccgaatcc acactttcga tcgaagaggt tctcgacgag
     6121 ctgatcgctc cgctcgggat tccggcgatc gccaacgttc cggtcggtca cggcaagcac
     6181 atggcgacca tgcccctggg ggcacatgtg cggctcgacg gcgaccggaa gaagcttgaa
     6241 gtgaccgagc cggcggttga accggcctag cccgaaacga cccactggag agagatgaat
     6301 tccaagagaa tcaggaatat tgcggcggcc gtaattgcgg ccctggccct gctgggcgcg
     6361 atggccctgc cggccgccgc cgacgagcaa ggtgacgccc ccgtgctcag catcggctgg
     6421 gcccaggacc cccagaccct gaaccccttc atcggactcg atgaggagga ctacacgatc
     6481 tgggcgatca actgggacct gctggtcaac ttcgacccgg aagacctctc gcccgcaccg
     6541 ggcatcgccg agagctggaa ggtgtccgac gacaagaaga cggtgacctt caagatcgcc
     6601 gatcgcaagt ggtctgacgg cgtgccggtt acctccgccg acgtgaagtg gtcgctggag
     6661 acgctgggct cggaaggtga gctgttcacc agctacacca acaacgtcac ttcgatcacg
     6721 acgccggaca agaagacggt cgtgatcaag accagcaagc cggacgcacg gattgtcggc
     6781 ggcctcctga tctacatcct gcccaagcac atctggggca aggtgccgct ctcggaactc
     6841 accaccacct atcagccgag cctgcccctg gtcggcaccg ggccgttcat cgtcaccgag
     6901 ttcgaccgcg gtcgcctgat caagatggac cagaacccgg aattcgatgg aaagaagccg
     6961 aagttcggcc agctccagtt catcaagtac ggcaatcagg acgcggtcga gcgcgccctg
     7021 cagctgggag agatcgacct tgtgccggag gttcaggcct ccaccttcaa gcggctcggc
     7081 gagcaggaca acatcaagac ggttgacagt ccttcaccgg ctttcacgga actggccttc
     7141 aacctctgtc cggaaaagct ctgccccgac gcccaggtga acccggcgat tcgtgacctc
     7201 cccgtgaggc aggcgatcgc ctactcgatc gaccgtgaac ggatcaacca gatcgcggcc
     7261 tcggacacct ccttcgtggc gaacgggctg ctgccgtcct attacaagtc cttctacgag
     7321 gacccggaga acacataccc gttcgacccc gagaaggcga agcagatcct cgatgacgcc
     7381 ggctacctgc cgggcgacga cgggatccgc gagaaggacg gggaccgtct ctcgttcaac
     7441 ctctacaccc ggaccaggtc ggagtccgcc tacaacgcgc aggccgccaa gctggtcgcg
     7501 gaaatggccc gtgacgtggg catcgagttc aaggtgcagg aggtcagcgt cgacaagctg
     7561 accgagctga ccgtccgtca ggtggacggc aagcccgccc ctgacttcga cgcgttcatc
     7621 tggggctggg gcggcgacgc ctacgacccg agcttcctgc tcagcctgat gaccaccggg
     7681 gagatcggcg gttcgtcgga ctccttctac tcgaacgccg aatacgacaa gctcttcaac
     7741 gagcagtccg gcgagttcga cgtcgaaaag cggaaggaga tcatccaccg gatgatcaac
     7801 cggctgcagc aagacctgcc ctacatcgtc ctcaccgagg accccaacct gcaggcctac
     7861 cggaccgacc ggatcgccaa cgtcgagccg acctgtcctt ccggggatgg cgacatcttc
     7921 tgtgagcagg ccggctatgc gcccctgctg gatctcgctc cgggatcgtc ttccggcgac
     7981 gagggcagca gcggcaacgg cgtgctgatc gcgatcatcg cggcgctggt aatcgccgtg
     8041 ctcgccttcg tgctgatccg tcaccggcgc cgccgggacg aaccgctcga ggtcgaggaa
     8101 gagacgctcg aggtcgagga gcagccggac ggcactgacg gagaggagaa gtgagcctca
     8161 gttgagcgta cgctggctca gcggcaaggt cgcggcagca gctctcacgc tgctgttcgt
     8221 gatcgtgttc aacttcttcc tcttccgggt catgggcgac ccgaccgacc agcttgccag
     8281 gctgccgcgg gcgaccccgg aagagatcca gaagttgcga ggccagtacg gcctcgacaa
     8341 gccaatgctg ggccagttca ccgactacat gggggacacc gcccgcctcg acttcgggat
     8401 cagccagagg tcgaggcgga cggtgaccga cgaaatcaag gacgcccttc cctggaccct
     8461 gctgctggtc ggcaccggga ccctgctggc gaccctgctt ggttcgtgga tgggggtgaa
     8521 ggccgctacg cggcgcggaa aacacagcga ccaggccctg ctgggcttca acctgttcac
     8581 atacgcgtcg ccggaatact ggatcgggat catcctgatc ctcgtcttcg cggtctggat
     8641 accgatcctg ccatccgggt tacaagtcac tccgggaacg gagttcagca acttcttttc
     8701 ccaggccctc gatgtcgcaa agcatctgat cctgcccgcc accgcgatga ccctgatgct
     8761 gatcggccag tacttcctga tcatgcgctc ttccatggtc gacgtgctga ccgaggactt
     8821 catcaccgtc aagcgggcga ccggaatgcg ctgggacagg gtcgtaaacc ggcatgccgt
     8881 gccaaacgcc ctgctgccgc tggtcacgct ctcggcgatc cagttcgggg cggtcgtagc
     8941 cggcgcgatc accatcgaga cgatcttctc ctggcccggc ctgggcgagc tcacctacca
     9001 ggcgatcaat gacaaggact tccccgttct gcaggggacc ttcctgttct tctcgttcgc
     9061 ggtgatcctc gccaacctcg ccgccgactg cctctacttc ttcctcgacc cgagggtgca
     9121 gggccgatga gcgggcggga ggcagtacgg gtgctgttca gctcctggcg ggggcgcttc
     9181 ggcatcgcgg tcctcgtcgt gaccgtgttc gtggcgatct tcggcagcgc cctggccccg
     9241 tatgacccga ccgcctccag cctcgacgtg ctgcagccgc ccagcttcga tcatctgctc
     9301 ggaaccaccg agagcgggga ggacgtcttc tcgcagctcc tggtcggcac ccggacctcg
     9361 gtcgtcgtcg gcttcgcggc agccgtcatc tccgcggtgc tcggctccgc ggtcggcatt
     9421 ttcagcggtt acttcggcgg ctggaccgac cgcatcctgg actcgctgga gaactggttc
     9481 ctcgtgatcc cgaccctgcc gctgatgatc gtcctggccc ggctgctcga cccgtcgcta
     9541 ggcgttctcg tcgcggtgat cggcctcacc tcctgggccg ggaccggacg catagtcagg
     9601 gcccaggtga tgaccctgcg cgagcgtgca ttcgtcgaac gggcaagggc cctcggcgcg
     9661 ggcgacacct acatcatccg cacccacgtg ctgccgaaca cgctgccgct gatcttcgcc
     9721 aacacggtgc tgatcatcgc ggtggcgatc ctttcggaag ccgcgctttc cttcctcggg
     9781 ctcggcgacc cgtcccggat ctcctggggc tcgatgctgg agaacgcctt cggctccggt
     9841 gccccttcgg ccggtgcctg gtggtacgtg atcccgccgg gactctgcat cacgatcctc
     9901 gtgctggcgg tcaccctgat cggcttcctc tacgaggaat acatcaaccc ccggctggcg
     9961 atggaccgcg aatcatgagc tcgatcctga agatccgcga ccttcatgtt gcctaccagg
    10021 gcaaggaaat tgtcgagggg gtggacctgg acctggcccg cggcgagtcg ctcggactgg
    10081 ccggggagtc gggctgcggc aagaccacca cggctctggc ggttatgaaa ctcctgcccg
    10141 aaggcttcac ccagtcgggt tcgatcacgc tcagtccgcc cggtgaggcc gagccgatcc
    10201 gtatcgaccg gaggaccgag gccggaatga gggttgtgcg ctggcgccat gtgtcactcg
    10261 tgtttcaggg tgcgatgaac tcgctggacc cggtcaagcg ggtcgggccc cagatcgaag
    10321 aggtgatcgt tctccacgag ggccgttccg gccggatcag cggacggatc gacgaactgc
    10381 tcggcacggt cggcctgacc ccggcgatcg cccgccggta cccgcatcag ctttccgggg
    10441 gccagaagca gcgggtgatg atcgccctgg ccctggcctg cggcccttcg gtggtgatcg
    10501 ccgacgagcc gacgaccgcg cttgatgtgg tcatgcaggc ccaggtgctc cagctgctgg
    10561 agaagctgag ggaggagctc ggtctcgccc tggtgctgat ctcccatgac ctgggggtga
    10621 tggcggagac ctgcgaccgg atcgcgatca tgtacgccgg gcggatcgtg gagaccggtc
    10681 cgaccgacca ggtgctcgcc gacccgcagc atccgtacac caggcggctg ctcgagtcat
    10741 tgccggtgat cggcggcgag cggggcctgg cgccacccat ccccggcatc ccgccggctc
    10801 cgggcgacgt cgtcgaaggc tgcgccttca ggccccggtg cccctacagc gaggaccgct
    10861 gcctccagga cccggcgctc aggcagatcg gcgacttgcg gtcggccgcc tgtcacttct
    10921 cgccgtggag tagctggccc gaggtggccg cgaccggcaa ggagaccgcc aggtgagcgt
    10981 cgaagcgccg acaaccctga tggaggtcaa ggatctcgaa gtggccttcc gccaccaccg
    11041 cagcacgctc agggcgctgg acggcgtctc cctcgagtgg aagcccggcg agatcctcgg
    11101 tcttgtcggc ga
//
LOCUS       DAOTIP010000003         9320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_8819_length_9320_cov_2.997949, whole genome shotgun sequence.
ACCESSION   DAOTIP010000003 DAOTIP010000000
VERSION     DAOTIP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_9320_cov_2.997949"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="P5138_00130"
     CDS             complement(<1..864)
                     /locus_tag="P5138_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV59012.1"
                     /translation="MAISEKLRLQNMINGEFVDPVDGGSEEVINPSTGEVIAQSPLSG
                     AEDVNRAVAAAKAAFENGWATTTPRERSDRLLALADAIIENGDELTDLESADAGKPRQ
                     AFIDEELDTTADHIRYFATAARNLEGKAAMEYLEGHTSFIRREPVGPVAQITPWNYPL
                     MMAAWKLGPALATGNTLVLKPAESTPVSTLHTLARICAEVFPPGVTNFIGGHGDPAGS
                     TLVTHPDIKMVSLTGSIGTGKWIAKAAADSLKRVHLELGGKAPVIVFDDADLDAVYET
                     IAGTALFNAGQD"
     gene            complement(900..2021)
                     /gene="ald"
                     /locus_tag="P5138_00135"
     CDS             complement(900..2021)
                     /gene="ald"
                     /locus_tag="P5138_00135"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRV59013.1"
                     /translation="MKVGVIKEIKQDEYRVAITPAGVREFVEHGHEVLIEDGAGEGSA
                     ISNTSYEAQGARILPDAGAVFDAAEMIIHVKELQASEIEMLKPEHLLFTYLHLAPDPE
                     QTAGLMGSGATCVAYETVEDAEGRLPLLAPMSEIAGRIATQAGAFMLEKPLGGRGVLL
                     GGVPGVAAANVVVIGGGVVGTNAAFIAMGMAADVFILDRSIDRLRELDLHYAREASTV
                     YSTTLAIEELLPKADLVIGAVLVHGARAPWVVNREQLGLMKPGAVLVDVAIDQGGCFE
                     TSRPTTHRDPTYEVDGITHYCVANMPGAVPITSTQALTNATLPYALTLADNGIQAAVQ
                     RDPGLKPGVNVAAGKVTHEGVAEGTGQQYVPVEEALGWV"
     gene            complement(2093..3475)
                     /locus_tag="P5138_00140"
     CDS             complement(2093..3475)
                     /locus_tag="P5138_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRV59014.1"
                     /translation="MSTATPSQTAVENLQEQARRHLWMHFSRMGGYSDEHEIPIITRG
                     DGAYVYDSHGKRYLDGLSGLFCVNAGHGRTELAEAAARQYEELDFFVLWSYAHPRAIE
                     LATKIASMTPGDLNRVFFTSGGSEAVESALKLARNYHRMRGNGQKAKHIAREVAYHGT
                     SLGALSATGITNLRSQFEPLAPGGCHVPNTNIYRWPEEREPVWAADAIEERILFEGPE
                     TVSAVILEPVQNAGGCFVPPDGYFQRVREICDKYDVLLISDEVICAWGRLGHYFGCDR
                     FGYQPDIITSAKALTSSYVPMGAMIASDKVAEPFMEGNASFAHGFTFAGHPVAAAVAL
                     ANIEIFEREDLCGHVLAKEGEFRGMLESLRDIPIVGDVRGAGYFQAIELVKDQDSKES
                     FSREESEKLLRGFLSGALYERGLICRADDRGDPVVQFSPPLICDTEQFEEMHDVLRPV
                     LTEAAAMMRN"
     gene            3686..5242
                     /locus_tag="P5138_00145"
     CDS             3686..5242
                     /locus_tag="P5138_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRV59015.1"
                     /translation="MESAEVSPGPGPASKTDQGEQPHLKADAISFTDGLAVALASTAP
                     AYSLAAVIGSIVVIVGLQAPAALLVAFVPMFFIASAFFYMNKADQDCGTCFAWVTRAI
                     GPQSGWMTGWAICVTGILVVGSLADVAASSFFDLIGADGLMNSTAAVTSTAVLLMLIM
                     TGVCVWGTEISANLQKVLMVVQIVPLMLFIVVALVKVWGGDAPAGSIDPSLSWLNPFN
                     IAAGELTGAMLLAVFIYWGWESAVNLNEETEGDADKPGLAGLISTTLLLITYVGVAFA
                     IVAWGGVSGAEKFNNDATILATYAKEVLGGTLGLLVLLSVVVSALASTQTTILPASRT
                     SLSMARAEAMPHAFGEISGRFFTPVVSTVTIGGLAILWYVPSKLISENFLVDSLSALS
                     LMIAFYYAMTGYACAFYYRRELLKSVRNFLFVGVAPVIGAVLLTYLFVKSLVDWSDPA
                     NSASGSSWFGFAPPAVIGIGFLLLGAILLFAWRRHQEEPFFRLRRQIADPGLLSGRAE
                     PAAALTGSDD"
     gene            5432..6658
                     /locus_tag="P5138_00150"
     CDS             5432..6658
                     /locus_tag="P5138_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV59016.1"
                     /translation="MEGSSDLPPTDPESDQPGETAGNTARSGSGGVRLEGVTKKFGDF
                     AAVHDMDLDIADGEFFTMLGPSGCGKTTTLRMIAGFEQPTTGKVLIDGSDVAGLPAYK
                     RPTNTVFQSYALFPHMTLEENVGFGLKRQKVPKAEIKKRVAGELERVGLSREAKRKPN
                     QLSGGQQQRVALARALVNLPKVLLLDEPLGALDLKLRKGLQVQLKEIQRDVGITFVYV
                     THDQEEALTMSDRIAVMNEGMIEQCADPETVYDRPSTTFVAGFIGVSNLMPAVAKGPG
                     EIEIADGPTVPTADAAQFSSGEKCFAVVRPEKLTVSLPENAPGDQPGIDGIVESSLYL
                     GTSTQMAIKVADDVNMTVLVPNASEDARQHLPGGGAKVRLNWLPEHMHLVAESDPGGS
                     DADQTSTDQVTEGVNS"
     gene            6655..7809
                     /locus_tag="P5138_00155"
     CDS             6655..7809
                     /locus_tag="P5138_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV59017.1"
                     /translation="MTNIKMKQIRLLVLGVLALVASLVLVACGGGGGLEGSDNSNVTV
                     AEGGDPTGSLNISNWPLYIDGKTISNFQNETGIKTTYTEDVNDNNEFFGKVQPLLSQG
                     DSGGRSIIVVTDWMAKKMYDLGYIQKIDHDKLPTVFNHLIPSLQHPSFDPDREFSVPW
                     QSGMTGLVVRKDLAPDVKSINDLFDPKYKGKVTMLTEMRDTVPLVMAADGIDPVDAST
                     QDWLDAIDKIKKASDSGQIRRFTGNDYVQDMAKGDVVAAIGWSGDAVQAQADNKNIQY
                     VQPEQGCSIWSDNMLIPVGAPNPAAAYKFMNYVYQPEVQANITQYVNYVSPVEGVKPI
                     LEKRDPAIANNELIFPSEEFVKNCFNQVSPPGDEEQQREVEQAFQDVVTG"
     gene            7865..8734
                     /locus_tag="P5138_00160"
     CDS             7865..8734
                     /locus_tag="P5138_00160"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV59018.1"
                     /translation="MTSVRQRLVPYWLLAPGLIWLIVFFVIPMYYMGELSLKTGSIDT
                     GYQLTWAFSNYTDAISQYSTQFFRAFYYAGVATLLCLLIGYPLAYAIAFKAGRWKNLM
                     LFAVVAPFFTTYLIRTIAWKTILDDSSPVVSFLKTIHLVPQDGRVLATSAAVIAGLTY
                     NFLPFMVLPLFAALERVDIRLLEAGKDLYASATQTFLRITLPITMPGIVAGVLLTFIP
                     ASGDYVNATFLGGTNQAMIGNVIQSKYLVVRDYSEAAALSFTLMAIILVALIVYIKFA
                     GTEALMGDEEEAE"
     gene            8731..>9320
                     /locus_tag="P5138_00165"
     CDS             8731..>9320
                     /locus_tag="P5138_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV59019.1"
                     /translation="MRAWRWLRENAVMIVGILALLYMLTPIFIIAIFSFNDPTGRYNF
                     EWVGFTTQYWQHPFEITELTSALEVSIRLAFFTAIGATILGTLIAIALVRYQFFGRRA
                     ANLLIVIPMATPEVVIGASLLSFFLTLNIFELGFQTLLIAHIMFSVSFVVIVVRSRLI
                     GFDRRLEEAAQDLGANAFTTFRKVTLPLIAPGVVSAA"
BASE COUNT         1594 a         3055 c         2957 g         1714 t
ORIGIN      
        1 gtcctggccg gcgttgaaga gtgcggtgcc ggcgatcgtc tcgtagaccg catcgaggtc
       61 ggcatcgtcg aagacgatca ccggagcctt gccgcccaac tccaggtgga cgcgcttgag
      121 cgaatccgct gcagccttcg cgatccactt tccggttcca atcgaaccgg tcagcgagac
      181 catcttgata tccgggtggg tgaccagggt cgagccggcc ggatcgccgt ggccgccgat
      241 gaagttggtc accccgggcg ggaagacctc ggcgcagatc cgggccaggg tgtgaagggt
      301 tgagaccggg gtcgactcgg ccggcttcag gaccagtgtg ttgccggtcg caagcgcggg
      361 tccgagcttc cacgccgcca tcatcagggg gtagttccag ggggtgatct gggcaaccgg
      421 accgaccggc tcgcgccgga tgaaggaggt gtggccctcc aggtactcca tcgccgcctt
      481 gccctcgagg ttccgggcgg cggtcgcgaa gtagcggatg tggtcggcgg tggtgtcgag
      541 ttcctcgtcg atgaaggcct ggcggggctt gccggcgtcg gccgattcga ggtcggtcag
      601 ctcgtccccg ttctcgatga tcgcgtcagc cagggcgagc aaacggtcgg agcgctcgcg
      661 cggggtggtg gtcgcccagc cgttctcgaa agcggccttg gcagcagcaa ccgcccggtt
      721 cacatcctcc gctccggaga gcggcgactg cgcgatcacc tcgccggtcg aaggattgat
      781 cacttcctcc gagccgccgt cgaccgggtc gacaaactcg ccattgatca tgttctgaag
      841 ccgcagcttc tcgctgattg ccatctctct cctgttcctg cctgggtgtt accgggtact
      901 cagacccagc cgagcgcttc ttcgaccggc acgtactgct ggccggttcc ttccgcgacg
      961 ccttcgtggg tgaccttgcc ggccgccacg ttgacgcccg gcttgaggcc ggggtcccgc
     1021 tgaacggcgg cctggatgcc gttgtcggcg agggtcaggg cgtagggcag cgtcgcgttg
     1081 gtgagggcct gggtcgaggt gatcggcacc gcaccgggca tgttggccac gcagtagtgg
     1141 gtgatgccgt cgacctcgta ggtcgggtca cggtgggtgg taggcctcga ggtctcgaag
     1201 cagccgccct ggtcgatcgc cacgtcaacc agcacggcgc ccggcttcat cagcccgagc
     1261 tgctcgcggt tgacgaccca cggggcgcgg gcgccgtgaa ccaggaccgc gccgatcacc
     1321 aggtcggcct tggggaggag ctcctcgatc gccagggtgg tcgagtagac ggttgatgcc
     1381 tcgcgggcgt agtggagatc gagctcgcgc aaccggtcga tcgaacggtc gaggatgaac
     1441 acgtcggccg ccatccccat tgcgatgaag gccgcgttgg tgccgaccac gccgccgccg
     1501 atcaccacga cattggccgc ggccacaccg ggcactccgc caagcaggac cccgcggccg
     1561 ccgagcggct tctcgagcat gaaggcaccg gcctgggtcg cgatccggcc ggcgatctcg
     1621 ctcatcgggg caagcagcgg cagcctgccc tcggcatctt cgaccgtttc gtaggcgacg
     1681 caggtggccc cggaacccat cagccccgcg gtctgttccg ggtcgggggc caggtgcaga
     1741 taggtgaaga gcaggtgttc cggcttcagc atctcgatct cggaagcctg gagttccttg
     1801 acgtggatga tcatctcggc cgcgtcgaac accgcccctg catcgggaag gatccgcgcg
     1861 ccctgggcct cgtaggaggt gttcgagatt gcactgcctt caccggcccc gtcctcgatc
     1921 aggacttcat ggccgtgctc gacgaactcg cgtacgcccg ccggcgtgat cgcgacccgg
     1981 tactcgtcct gcttgatctc cttgatgacg ccgaccttca tctcttacct cgtcctctct
     2041 ggtggtccgc tgggatgact tctccgccgg gcggggtgta tgggtgaatg gatcagttcc
     2101 tcatcattgc cgcggcctcg gtgaggacgg gcctgaggac gtcatgcatc tcctcgaatt
     2161 gctcggtgtc gcagatcagt ggcggcgaga actggacgac gggatcgccg cggtcgtcgg
     2221 cccggcagat caggccgcgc tcgtagaggg cgcccgacag gaagccgcga agcagcttct
     2281 cggactcctc gcgactgaag ctttccttgc tgtcctggtc cttgaccagt tcgatcgcct
     2341 ggaagtagcc cgccccgcgc acgtcaccga cgatcgggat gtcccggagc gactccagca
     2401 ttccgcggaa ctcgccttcc ttggccagca cgtggccgca gagatcctcg cgttcgaaga
     2461 tctcgatgtt ggcgagcgcc actgccgcgg cgaccgggtg accggcgaag gtgaacccgt
     2521 gggcaaacga ggcgttgccc tccatgaacg gctcggcaac cttgtccgag gcgatcatcg
     2581 cgcccatcgg cacgtaggag gaggtgagcg ccttggccga ggtgatgatg tccggctggt
     2641 atccgaaccg gtcgcagcca aagtagtggc cgagacgacc ccaggcgcag atcacctcgt
     2701 cggagatcag caggacgtcg tacttgtcgc agatctcgcg gacccgctgg aagtatccgt
     2761 ccgggggaac gaagcagccg ccggcgttct ggaccggctc gaggatcacc gcggaaacgg
     2821 tttccgggcc ctcgaagagg atccgctcct cgatcgcgtc cgcggcccag accggctctc
     2881 gttcctccgg ccagcggtag atgttggtgt tcggaacgtg gcagccgccg ggagccagag
     2941 gctcgaactg ggagcggagg ttggtgatcc cggtcgcgga gagggcgcca agggaggtgc
     3001 cgtggtaggc gacttcacgg gcgatgtgct tcgccttctg cccgttgccg cgcatgcggt
     3061 ggtagttgcg ggccagcttc agtgccgact cgaccgcctc gctgccgccg ctggtgaaga
     3121 agacccggtt gaggtcaccc ggggtcatcg aggcgatctt ggtcgccagt tcgatcgccc
     3181 gcgggtgggc gtagctccag agcacgaaga aatcgagttc ctcgtactgg cgggcagcgg
     3241 cttcggccag ttcggtccgg ccgtgaccgg cgttcacgca gaagagcccg gaaagcccgt
     3301 cgaggtagcg cttgccgtgg gagtcgtaga catacgcccc gtcaccccgg gtgatgatcg
     3361 ggatttcatg ctcgtcgctg tacccgccca tgcgggagaa gtgcatccag aggtggcgcc
     3421 tggcctgttc ctgaaggttc tcgactgcgg tctggcttgg tgttgcggtg gacacggact
     3481 cctccttggg tcaaggcccg gaaggcgggc cccgttgccg cgctgcggcc acggtggttt
     3541 ctggctccac cctatgtcgg cccgatcaca ctggcaagcc gacgaaacgt caaaagacgc
     3601 cgatcggcca ttacctcccg tatttccctg gcctgggcgt tgcgggtgga tggcaagacg
     3661 ccgacaacgc gactaggttc tccacatgga gagcgccgag gtttcacccg gtccgggccc
     3721 tgcctcgaag acggatcagg gagagcagcc gcacctcaag gcggacgcga tcagcttcac
     3781 cgatggcctg gccgtcgccc tcgcctcgac ggcgccggcc tactcgctgg cggccgtgat
     3841 cgggtcgatc gtggtgatcg tcggcctgca ggcaccggct gccctgctgg ttgccttcgt
     3901 cccgatgttc tttatcgcct cggccttctt ctacatgaac aaggccgacc aggactgcgg
     3961 tacctgtttc gcctgggtca ccagggcgat cggtccgcaa tcgggctgga tgactggctg
     4021 ggcgatctgc gtgaccggga tccttgtggt ggggtcactg gccgatgtcg ccgcctccag
     4081 cttcttcgat ctgatcggag ccgacggcct gatgaacagc accgccgcgg tcacctcgac
     4141 cgccgtgttg ctgatgctga tcatgaccgg cgtctgcgtc tggggcaccg aaatatcagc
     4201 caacctccag aaggtcctga tggtggtcca gatcgtgccc ctgatgctct tcatcgtcgt
     4261 cgcactggtc aaggtctggg gtggtgacgc tcccgccggt tcgatcgacc cctccctgtc
     4321 ctggctgaac ccgttcaaca tcgctgccgg cgagctcacc ggcgcgatgc tgctcgcggt
     4381 attcatctac tgggggtggg agagcgcggt caacctgaac gaggaaaccg aaggtgatgc
     4441 cgacaagccg ggcctggccg gcctgatctc gaccaccctc ctcctgatca cctacgtcgg
     4501 ggtcgccttc gcgatcgtcg cctggggtgg cgtcagcggc gccgagaagt tcaacaacga
     4561 cgcgacaatc ctggccacct acgccaagga ggttctcgga ggcaccctcg gacttctcgt
     4621 cctgctgtcc gtcgtggtct cggccctggc ctccacccag accaccatcc tcccggcctc
     4681 gcgcacctcg ctttcgatgg cccgcgccga ggccatgccc cacgcctttg gcgagatctc
     4741 cggccgtttc ttcacccctg tcgtctcgac cgtgacgatc ggcgggctgg caatcctctg
     4801 gtatgtcccc tcgaagctga tcagcgagaa cttcctcgtc gactcgctct cggccctctc
     4861 gctgatgatc gccttctatt acgcaatgac cggctacgcc tgcgccttct attaccggcg
     4921 ggaactcctc aagtcggtcc ggaacttcct cttcgtcggc gtggctccgg tgatcggtgc
     4981 cgtgctgctc acctatctct tcgtcaagtc gctggttgac tggagcgacc cggcaaattc
     5041 ggccagcggc tcctcctggt tcggcttcgc gccgccggcg gtgatcggca tcggcttcct
     5101 gttgctggga gcgattctcc tcttcgcgtg gcggcgccac caggaagaac ctttcttccg
     5161 cctccgacgc cagatcgccg atccgggcct gctctcgggg agagccgaac ccgcggctgc
     5221 cctcaccggc agcgacgact gaatccggcc tgtcgccgcc ctaaataccc ggtatcggaa
     5281 gggtccgcat tggacaaccg gaccattcac gtcgcgctta ttggacaaaa cgtggttgcg
     5341 gaggtcccct tgtttcgcat acggttcaaa ccgaaagtcc ccggaagcgc gggttcgtgg
     5401 ttccgggcag tccacctgag agaggggttc gatggaaggc tcgtcagatc taccgccgac
     5461 cgatccggaa tcggatcagc cgggggaaac agccgggaac accgcaagat ctgggagcgg
     5521 aggcgttcgc ctcgaagggg tcaccaagaa gttcggtgac ttcgccgcgg tccacgacat
     5581 ggacctcgac atcgccgacg gcgagttctt caccatgctc ggcccgtccg gctgcggcaa
     5641 gaccacgacc ctccggatga tcgccggctt cgagcagccg accaccggca aggtgctgat
     5701 cgacggatcg gatgtggcgg gactgccggc atacaagcgc ccgaccaaca cggtcttcca
     5761 gtcctacgcg ctcttccccc acatgaccct ggaggagaac gtcggcttcg ggctgaagcg
     5821 ccagaaggtc ccgaaggcgg aaatcaagaa gcgggtagcc ggcgaactcg agagggtcgg
     5881 gctctcccgc gaggcgaagc gcaagccgaa ccagctttct ggcggccagc agcagcgggt
     5941 cgccctcgcc agggccctgg tcaacctgcc caaggtgctg ctgctcgacg agccgctggg
     6001 cgcgctcgac ctcaagctcc gcaagggcct tcaggtccag ttgaaggaga tccagcgcga
     6061 cgtcggaatc accttcgtct acgtgaccca cgaccaggaa gaggcgctca ccatgagcga
     6121 ccggatcgcg gtcatgaacg aagggatgat cgagcagtgc gccgatccgg agaccgtcta
     6181 cgaccgcccc tcgaccacct tcgtcgccgg cttcatcggg gtttcgaacc tgatgcccgc
     6241 ggtagccaag ggcccgggcg agatcgagat cgccgacggc ccgaccgtgc ccaccgccga
     6301 tgccgcgcag ttctcgagcg gggagaagtg cttcgccgtt gtccggcccg agaagctgac
     6361 cgtttcgttg ccggagaacg cccccggcga ccagcccggg atcgacggca tcgtcgagag
     6421 ctcgctctat ctcggcacct cgacccagat ggcgatcaag gtcgccgatg acgtgaacat
     6481 gaccgtcctg gttcccaacg cttccgaaga cgcccggcag catctgccgg gcggcggcgc
     6541 gaaggtccgt ctcaactggc tgccggagca catgcatctg gttgccgagt ccgatccggg
     6601 cgggtcggat gctgatcaaa cgtccaccga ccaagtcact gaaggagtga attcatgacc
     6661 aacatcaaga tgaaacagat ccgtctgctc gtgctggggg tgttggcgct ggttgcttcg
     6721 cttgtgctgg tcgcctgcgg aggcggcggc ggactggaag ggtcggacaa ctccaatgtg
     6781 acggtggccg aaggcggcga cccgaccgga tcgctcaaca tctccaactg gccgctctac
     6841 atcgacggca agacgatcag caatttccag aacgaaaccg ggatcaagac gacctacacc
     6901 gaggacgtca acgacaacaa cgagttcttc ggcaaggtcc agccgctcct cagccagggt
     6961 gactccggcg gccgttcgat catcgtggtt acggactgga tggccaagaa gatgtacgac
     7021 ctcggctaca tccagaagat cgaccacgac aagctgccga ccgtcttcaa ccacctgatc
     7081 ccgagtctcc agcatccgtc cttcgacccc gaccgcgagt tcagcgtgcc gtggcagtcg
     7141 ggtatgacgg gccttgtggt ccgcaaggat ctggccccgg acgtgaagtc gatcaacgac
     7201 ctgttcgacc ccaagtacaa gggcaaggtc acgatgctga ccgagatgcg tgacacggtg
     7261 ccgctggtga tggccgccga cgggatcgac ccggtcgacg cgagcaccca ggactggctc
     7321 gacgcgatcg acaagatcaa gaaggcctcc gacagcggcc agatccgccg cttcaccggc
     7381 aacgactacg tccaggacat ggccaagggc gacgtcgtcg cggctatcgg ctggtccgga
     7441 gacgcggtcc aggcccaggc cgacaacaag aacatccagt acgtccagcc tgagcagggc
     7501 tgctcgatct ggtcggacaa catgctgatc ccggtcggcg ctccgaaccc ggccgccgcc
     7561 tacaagttca tgaactacgt gtaccagccg gaagtccagg cgaacatcac ccagtacgtg
     7621 aactacgtga gcccggtcga gggggtcaag ccgatcctcg agaagcgcga tcctgcgatc
     7681 gccaacaacg agctgatctt cccgagcgaa gagttcgtga agaactgctt caaccaggtc
     7741 tcgccgcccg gtgacgaaga acagcagcgg gaggtcgagc aggccttcca ggacgtggtc
     7801 accggctgac caagggaaac ggggaaagcg ctgacgaaca gatgaaaatg gtgaacagaa
     7861 accaatgaca tccgttcgtc agcgtctcgt cccctattgg ctgctcgccc ccgggctgat
     7921 ctggctgatc gtcttcttcg tcatccccat gtactacatg ggggagctgt cgctgaagac
     7981 cggctcgatc gacaccggat accagctgac ctgggccttc agcaactaca cggacgcgat
     8041 ctcccagtac agcacccagt tcttccgggc cttctactac gccggggtcg cgacgctgct
     8101 ttgtctgctg atcggctacc cgctcgcata cgcgatcgcc ttcaaggcgg ggaggtggaa
     8161 aaacctgatg ctcttcgcgg tggtggcgcc gttcttcacc acctacctga tcaggacgat
     8221 cgcctggaag accatcctcg atgactccag cccggtggtc agcttcctca agacgattca
     8281 cctggtcccg caggacggcc gggtgctcgc cacctcggcc gcggtgatcg ccggcctcac
     8341 ctacaacttc ctccccttca tggttctgcc gctgtttgcc gcgctcgaac gggtggacat
     8401 tcgtctgctc gaggctggca aggatctgta cgcatccgcc acccagacct tcttgcggat
     8461 aaccctgccg atcacgatgc cgggcattgt cgccggagtc ctgctcacct tcatcccggc
     8521 ctccggcgat tacgtgaacg cgaccttcct cggcggcacc aaccaggcga tgatcggcaa
     8581 cgtcatccag tccaagtacc tggtcgtgcg cgactactcg gaggctgcgg ctctttcgtt
     8641 caccctgatg gcgatcatcc tggtcgccct gatcgtgtac atcaagttcg ccggcaccga
     8701 agccctgatg ggggatgagg aggaggcaga atgagggctt ggcgctggct gcgcgaaaac
     8761 gccgtgatga tcgtcggaat tctggccctg ctctacatgc tgacgccgat tttcatcatc
     8821 gcgatcttct cattcaacga tccgaccggc cgctacaact tcgagtgggt cggattcacc
     8881 acccagtact ggcagcatcc gttcgagatc acggaattga ccagcgcgct cgaagtgagc
     8941 atccggctgg ccttcttcac cgcgatcggg gcgaccatcc tcggaaccct gatcgcgatc
     9001 gcccttgtca ggtaccagtt cttcggcaga agggcggcga acctgctgat cgtgatcccg
     9061 atggcgaccc cggaagtcgt gatcggcgct tcgctcctgt ccttcttcct caccctgaac
     9121 atcttcgaac tcggcttcca gaccctgctg atcgcccaca tcatgttctc ggtcagcttc
     9181 gtcgtgatcg tggtgagatc gagactgatc ggtttcgacc gccggctcga ggaagcagcc
     9241 caggacctcg gcgcgaatgc gttcaccacc ttccgcaagg tgaccctgcc cctgatcgcc
     9301 ccgggagtcg tttcggccgc
//
LOCUS       DAOTIP010000004         9312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_8829_length_9312_cov_3.548882, whole genome shotgun sequence.
ACCESSION   DAOTIP010000004 DAOTIP010000000
VERSION     DAOTIP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_9312_cov_3.548882"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="P5138_00170"
     CDS             <1..930
                     /locus_tag="P5138_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HRV59020.1"
                     /translation="LRTDVHSSVGTAIQQIASLRHSLAATLKTLRLRAASAGTHPFAV
                     WEEIVVSAGERYQFVHGSMRELARREPTFALHVHVGIGDSDTAINVHNRMRVFLPVLL
                     ALSANSPFWQGRDTGLSSARTPLFQAFPRVGVPRPFHSYRQYVDNVDLLIRTNAIPEP
                     TFLWWDVRPQPSLGTIEVRVMDSQTRNEDTAALVAMTQCLARREALDSQLSPAQIDSQ
                     ESISENRFLAARDGIEAELIDSERELRVPVPKIVDRLVDALRPHAQDLDCEPELEHCL
                     DLARENGAVRQRRISREVPSLAQMVSILADELE"
     gene            940..2199
                     /locus_tag="P5138_00175"
     CDS             940..2199
                     /locus_tag="P5138_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV59021.1"
                     /translation="MTSSVTFRAAIAAVVLLALGTLVIDGCARDEEDGTTRTLRVYVS
                     QPLKGDRGGEDMLRAVEIANENAGERIGAAKIEIVGMNDSDESGNWVASLVRRNARRA
                     ANDPAAVAYIGDFDSGATEVAMPILNRAGMLQVSSSSTAVKLTKPTPNVGERIRPTGI
                     RTFGRVVPNDNVQAAALALFMDEESVDEVFVVDDGDTYGEGLRYRFGRVAGHTGIDIK
                     GAATLTREAQIGNIARQVTGSGADALLFAGSNLDLARDLFETVHRDNNFIKLFGGDGL
                     SLSSFLESIGDTGLDTYITAPQLPAGNYAQSGEAFLCGFIEQYGRPAEPMAVFAYEAG
                     RVVLDSTRRGARGEISSEPIDVLRTNTRNAFFDTSERASPLGSYSIDANGDTTLTFYG
                     AYRVENGQLVLGRTIDVPPSLLMEDEE"
     gene            2196..2987
                     /locus_tag="P5138_00180"
     CDS             2196..2987
                     /locus_tag="P5138_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HRV59022.1"
                     /translation="MSTLLACLAIFLGGCLITGISIFLIRRFVPENLKGSEERREPVF
                     AFAGVLYALVIGFVLAYALDGYQTAETAAGTEADSVSSLSRSATLFDAESRDRIGHEL
                     ICYARAVIDDEWPAMSHGHRSELATAATDRLFQSFGKLGRNNADNSALSTSLERVSDL
                     QKARATRLLKSDTFLPTMFWVFMIAGGFMLILFATVLKGKEPIRGQIMFILPVSLLLI
                     CSVYLVFVFEQPFRGPNEVKPRAMERALVSVREFIPDPRADRPCP"
     gene            complement(3003..4787)
                     /locus_tag="P5138_00185"
     CDS             complement(3003..4787)
                     /locus_tag="P5138_00185"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HRV59023.1"
                     /translation="METSENKLKFTPFNLAVILAALFLGVGAGALVYRQYKIDHSKSK
                     SSKKSKQEGQNLNALAPVVGTEQGNGDPGTKYDAQFSTTSMWPKFNPDRHYYVTRCVP
                     GRVMVQVRSNPDVDVKVWANPAQSGRYAAEARPLPGQDFNVKILRADGPETYEVRCLP
                     SNFPEWRYKRFKKPPKGMFMVSSYLTPGKTGRVWLIVFDQDGTPRWWYSPPYNTLGGQ
                     VLYDGTVQLPRGFGDGFGQDARTATDIRDLDGNLVKTVRFKGAPTDGHEYVLLPNGNQ
                     LVMSYDPRYGVDLRPVGGRANRGVLDGAFQEQTPDGKVLWSWNSGDHIPLTGTPYRWW
                     IREWNNPHFDKNGKIRYDHFHLNSIEPWRDEYVISTRHTDSIWGIDKKTGKIVWKFGG
                     VKTPQSLKVVGEDPYGNYPISGNHDARMDGDILTVHDNGTHVKGRAPRALSYRIDLKN
                     RTATYIGALTDKAAAPSSHCCGSFRKLGTGWVVSWGDNPWVTGFNSKGQMAFRLGLPV
                     PAYRAVPVPATVSESDLDDGLDAMEPYPQMSNQPMTPLEFNDKHNLQDRTVPSRKLKP
                     IPEDNQDELGLTKSGKVAKSGNSGNSGN"
     gene            4927..5640
                     /locus_tag="P5138_00190"
     CDS             4927..5640
                     /locus_tag="P5138_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59024.1"
                     /translation="MPPLRKFSMTDTLQGMRVAFEKDRALVIRITLLFAFVIALSGLL
                     DLTGPAGIAISLGFFVLFGAMYQGLITALVCLPGKVEGLGELWQAVRSVLATLIWVAL
                     AYTGATVLGLLAFFVPGLMILTIWSVAGQVVVVERKGLSGAFRRSAELIRGNGFAVFG
                     FLLVIGIIALTISLLAILVARPLGTGVIGNFITPFLSNMFSAPFYALGLAALYNELTG
                     RGINPEPAPPGQETRDEGQ"
     gene            complement(5588..>6747)
                     /locus_tag="P5138_00195"
     CDS             complement(5588..>6747)
                     /locus_tag="P5138_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59025.1"
                     /translation="IGPLAGGYLSEWTWRAIFWINIPVAILSLIFIYISKPEETRRPQ
                     PIDYRGAVLVCIGMALAVLGLQQSSIWGWGNPVTWICIVGGLLVLAGFVAFEMKQEHP
                     LIRVAIFRDRSFAVDNVLLFLLSIAFVPLFFFASMYAQLSLDWQASEAGLYLLIFFSG
                     FATAAQFGGRILDSRGARPTVVLGCLLATVGFFLWAMKLTDLDGGSNAQWPFIVLTGA
                     GFGFLLGPVATDAVNRAPNTSYGEATGITQTVRNYGASVGMAVLGTILILRNKANIES
                     ELNGIVPTSEAHRIADQVSSAGSGGMSGSGGSSGSEKIYHAIQDAFAQSSKTVFLCMA
                     GVMAVCFLVALIGSPSGKMEEVFADEEEAPGAEAATGPRPGSPDPAEPAPD"
     assembly_gap    6748..6757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6758..7245)
                     /locus_tag="P5138_00200"
     CDS             complement(<6758..7245)
                     /locus_tag="P5138_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59026.1"
                     /translation="MAEKKSGYLVLVAMIFAVSMTFIDQTIVSISIPRIQEDLSLTST
                     GVQWVINGYLLSLAALFALGGKLADVFGHRRLVVIGVLIFAVSSAFCGFTPDTNIDET
                     WLIFFRVVQGAGAAMMFPAALAIVISSFPIADRGKAMAIFFGVTGAFTSMWMQEYKGG
                     AS"
     gene            7324..8211
                     /locus_tag="P5138_00205"
     CDS             7324..8211
                     /locus_tag="P5138_00205"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HRV59027.1"
                     /translation="MEILRTPEERFESIPDFPWEPEYREWEGMRLAHIDEGEGEPVLL
                     VHGEPTWGYLWRKVMGPLLDAGYRCIVPDLPGFGRSDKPVDDDWYTYDRHTAALVSLI
                     EELDLDGLTLVCHDWGGPIGLRAASIEVPERFTRLVAMDTGLFTGYQKMSDNWNHFRD
                     FIASHRDVRVDMPIRGGCATELSDEVVAAYEAPFPDVNYKAGIRTFPPMIPVTPEDPG
                     AEAGQEAAKYLIADDRPSLVLWADSDPALPMDPVGKTVQMLFPKAEPLTVIENAGHFL
                     QEDHGEQIGRIIADWLRKN"
     gene            complement(8198..8809)
                     /locus_tag="P5138_00210"
     CDS             complement(8198..8809)
                     /locus_tag="P5138_00210"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HRV59028.1"
                     /translation="MTPRATKLQAALTDPGEPEDSGPLRALRLGISNFMAKERIDMSA
                     MARQLDINRATLYRWVGSREQFLVEMIWHMTSASLDRLDRECDSTGPDRIIDVTIEFS
                     EEVIGNPGMKHWLATEGEAAMSLLTRAGSGYQPRLIAWVEDSIERQLAAGNMDLPEGV
                     EPYELAYVIERVMGGYIYLDLITGEEPDARRAEPLLRMLLSSS"
     gene            8973..>9312
                     /locus_tag="P5138_00215"
     CDS             8973..>9312
                     /locus_tag="P5138_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59029.1"
                     /translation="MSNEDKGSAPAKPALVLTSLILVAAVANLNLAVANVALPDIGKD
                     LDASQTGLNLVAVGFSLGLASSVLYLGALGDRYGRKMLLIIGTTVSIPAALLAGFAPS
                     VGVLFVARVIG"
BASE COUNT         1638 a         3056 c         2955 g         1653 t
ORIGIN      
        1 ctccggaccg acgttcattc cagcgtcgga acggcgatcc agcagattgc ctcgctcagg
       61 cacagcctcg ccgcgaccct gaagaccctg cggctgaggg ccgcctcggc cggaacccac
      121 ccgttcgccg tctgggagga aatcgtcgtg tcggccggcg agcgctacca gttcgtgcat
      181 ggctcgatgc gcgagctggc ccggcgggaa ccgacctttg ccctccacgt gcacgtcggt
      241 atcggtgact ccgatacggc gatcaatgtc cacaaccgca tgcgggtctt ccttccggtc
      301 ctgctcgccc tttccgccaa ctcgcccttc tggcagggcc gcgacacggg gctctcctcg
      361 gcccgaaccc cactgttcca ggccttcccc cgggtcggtg tgccccggcc cttccacagc
      421 taccggcagt acgtcgacaa cgtcgacctg ctgatccgga ccaacgcgat ccccgaaccc
      481 accttcctct ggtgggacgt caggccgcag ccttcgctcg gaaccatcga ggtgagggtg
      541 atggactcgc agaccagaaa cgaagacacc gccgcgctcg ttgcgatgac gcaatgcctg
      601 gcccgtcggg aagcccttga cagccagctc tcccctgcac agatcgactc ccaggaatcg
      661 atcagcgaga accgcttcct ggcggcgcgc gacggaatcg aggcggaact gatcgattcc
      721 gaacgtgagc tccgggttcc ggtgccgaag atcgtggacc gcctggtgga tgcgctcagg
      781 ccgcatgccc aggatctcga ttgcgaaccg gaactcgagc actgcctgga cctggcccgc
      841 gagaacgggg cagtgcggca gcggcggatc agccgggaag ttccatccct cgcccagatg
      901 gtcagcattc tggccgacga actggaatag tccccgatct tgacgtccag tgtcacgttc
      961 cgggcagcga tcgcggccgt agttctcctc gccctgggga cgctggtgat cgacggctgt
     1021 gcgagggatg aggaagacgg gaccactcgc accctgaggg tctatgtgag ccagccgctg
     1081 aagggcgacc ggggcggcga ggacatgctg cgggccgtcg agatcgcgaa cgagaacgcg
     1141 ggcgagcgga tcggcgccgc gaagatcgag atagtcggaa tgaacgattc cgacgaatcg
     1201 gggaactggg tcgcctccct ggtccggaga aacgcccgtc gcgccgccaa cgacccggcc
     1261 gccgtcgcct acatcggtga cttcgattcc ggagcgaccg aggtcgcaat gccgatcctc
     1321 aaccgggcgg gaatgcttca ggtcagttcg agttcaaccg ccgtgaagct gaccaagccg
     1381 accccgaacg tgggtgagcg gatacgtccg accgggatcc gcaccttcgg ccgggtggta
     1441 cccaacgaca atgtgcaggc ggcagccctg gccctgttca tggacgagga aagcgtcgat
     1501 gaggtgttcg tcgtcgacga cggcgacacc tatggcgagg ggctgcgtta ccggttcggg
     1561 agggtcgccg gccacaccgg gatcgacatc aagggtgctg caacgctcac ccgggaagcg
     1621 cagatcggga acatcgccag acaggtaacc ggttccggcg ccgatgccct gctgtttgcc
     1681 gggtcgaacc tggacctggc ccgggacctg ttcgagacgg tgcaccgcga caacaacttc
     1741 atcaagctct tcggcgggga cggactctcg ctcagctcct tcctcgagtc gatcggcgac
     1801 accgggctcg acacctacat aaccgccccg caactcccgg ccggaaacta cgcccagagc
     1861 ggcgaagcat tcctctgcgg attcatcgaa caatacgggc ggcccgccga accaatggct
     1921 gtcttcgcct acgaggccgg ccgggtggtt cttgactcga ccaggcgcgg tgcccgcggc
     1981 gaaatcagca gcgagccgat cgacgtgctc cggaccaaca cacgcaacgc cttcttcgac
     2041 acctcggagc gggcctctcc gctcggcagc tattcgatcg acgccaacgg tgacaccacc
     2101 ctcacctttt atggtgccta ccgggtcgag aacggacagc tggtgctcgg ccggacgatc
     2161 gacgtaccgc cttccctgct gatggaggac gaagaatgag cacactgctg gcctgtctgg
     2221 cgatcttcct cggtggctgt ctgatcaccg gcatatcgat cttcctgatc cgcaggttcg
     2281 ttccggaaaa cctgaaggga agcgaggagc gccgcgaacc ggtcttcgcc ttcgccgggg
     2341 tgctctacgc cctggtcatc ggcttcgtgc tcgcctacgc gctcgacgga taccagaccg
     2401 cggaaacggc ggccggcacc gaggccgatt cagtcagttc actctcccgg tcggccaccc
     2461 tcttcgacgc cgaaagccgg gaccggatcg gccacgaact catctgttac gcccgtgccg
     2521 tcattgatga cgaatggccg gcgatgtcgc atggccatcg ctccgagctg gccacggcag
     2581 ccaccgaccg gctgttccag tccttcggca agctgggccg gaacaacgcc gacaactctg
     2641 ccctctccac atccctcgaa cgggtgagtg atctgcagaa ggcgcgggcc acccgcctgc
     2701 tcaagtccga caccttcctg ccgaccatgt tctgggtgtt catgatcgcc ggcggattca
     2761 tgctgatcct cttcgccacg gtcctcaagg ggaaggaacc gatccgcggc cagatcatgt
     2821 tcatcctgcc ggtctcgctg ctcctgatct gctcggtcta tctggtcttc gtcttcgagc
     2881 agccgttccg cggaccgaac gaggtgaaac cgcgggcgat ggaaagggcc ctggtttcgg
     2941 tcagggaatt catcccggac ccccgggccg accgcccctg cccctagcaa tcgccggccg
     3001 ggctagttgc cggagttgcc ggagttgccc gacttggcca ccttgcccga cttcgtgaga
     3061 ccaagttcat cctggttgtc ctcgggaatc ggcttcagct tgcggctggg gacggttctg
     3121 tcctgaaggt tgtgcttgtc gttgaactcc agcggagtca tcggctgatt gctcatctgc
     3181 gggtacggct ccatcgcgtc gagaccgtca tcgaggtcgg attccgaaac ggtggccggg
     3241 accggcaccg cccggtaggc ggggaccggc aggccgagcc ggaaagccat ctggcccttt
     3301 gagttgaacc cggtcaccca ggggttgtcg ccccaggaga ccacccagcc ggttccgagc
     3361 ttccggaagg agccgcagca gtggctgctc ggagccgcag ccttgtcagt caatgccccg
     3421 atgtaggtgg cggtccggtt cttcaggtcg atccggtaac tcagcgcccg gggagcgcgg
     3481 cccttgacgt gggtgccgtt gtcatggacg gtcaggatgt cgccgtccat ccgggcgtcg
     3541 tgattgcccg agatcggata gttgccatag gggtcttcac cgaccacctt gagcgactgc
     3601 ggggtcttga ccccgccgaa cttccagacg atcttcccgg tcttcttgtc aatgccccag
     3661 atcgagtccg tgtggcgggt cgagatcacg tactcgtctc gccacggctc gatcgagttg
     3721 aggtgaaagt ggtcgtaacg gatcttcccg ttcttgtcga agtgggggtt gttccattcc
     3781 cggatccacc agcggtaggg cgtcccggtc agaggaatgt ggtcaccgga gttccaggac
     3841 cagaggacct ttccgtccgg ggtctgctcc tggaaggctc cgtcgaggac cccgcgattg
     3901 gcccggcccc cgaccggtcg caggtcgact ccgtagcggg ggtcgtaact catgaccagc
     3961 tggttgccgt tcggcagcag cacgtactcg tgtccgtcgg tgggcgcgcc cttgaaccgg
     4021 acggtcttga ccaggttgcc gtcgaggtcc cggatgtcgg ttgccgtgcg ggcgtcctgg
     4081 ccgaagccgt cgccgaagcc gcgcggcaac tgaaccgtgc cgtcgtagag gacctggccg
     4141 ccgagcgtgt tgtacggcgg gctgtaccac cagcggggcg tcccgtcctg atcgaagacg
     4201 atcagccaga cccggccggt cttgccgggg gtcaggtagg agctgaccat gaacatgccc
     4261 ttcggcggct tcttgaaccg cttgtaacgc cattccggga agttggaggg cagacaacgg
     4321 acctcgtagg tctcgggccc gtccgcgcgg aggatcttca cattgaaatc ctggccgggc
     4381 aggggccttg cttcggcggc gtagcgcccg gactgggccg ggtttgccca gaccttcacg
     4441 tccacgtcgg ggtttgagcg aacctgaacc atgacccgtc ccgggacaca gcgggtcacg
     4501 tagtagtggc ggtccgggtt gaacttcggc cacatggagg tggtgctgaa ctgggcgtcg
     4561 tacttcgtgc cgggatcgcc attgccctgt tcggtaccga cgaccggggc cagggcgttc
     4621 aggttctggc cctcctgctt cgacttcttc gacgacttgg acttgctgtg gtcgatcttg
     4681 tactgccgat atacaagcgc gcccgcgccg acgccgagga agagggcagc aaggatcacc
     4741 gcaaggttga atggcgtgaa tttgagtttg ttctcggagg tttccatgcc ggggaggggt
     4801 gaccggtttc ccattcgggc cggggatggc tacacaactc cgtgcgccat cccggccgcg
     4861 cctggggcga ctctctgaat ggtccgggaa ctataaaggc accccctgcg ggtagggttc
     4921 gcgcccatgc cgccgctccg caagttctcg atgaccgaca cgcttcaggg catgcgggtc
     4981 gccttcgaga aggaccgggc cctggtcatc cggatcacgc tcctgttcgc gttcgtgatc
     5041 gcgctcagcg gcctgctcga cctgaccgga cctgcgggga tcgccatttc tctcgggttc
     5101 ttcgtcctgt tcggagcgat gtaccagggc ctgatcacgg cgcttgtctg cctcccgggc
     5161 aaggttgagg ggctgggaga gctttggcag gcggtccggt ccgtcctggc aaccctgatc
     5221 tgggtggcgc tcgcatatac cggtgcgacc gtcctgggcc tgcttgcgtt cttcgttccc
     5281 ggtctcatga ttctcacgat ctggtccgtt gccggccagg tggtggtcgt cgagcgaaag
     5341 ggcctgtccg gcgcgttccg ccgaagcgcg gagttgatcc gcggcaacgg attcgcagtc
     5401 ttcggcttcc tcctggtgat cggcataatc gccctcacga tctcgctgct ggcgatcctg
     5461 gtcgccaggc cgctgggaac cggggtgatc ggcaacttca tcaccccctt cctcagcaac
     5521 atgttcagcg cacccttcta tgcgcttggc ctcgccgctc tctacaacga gctgaccggc
     5581 cgcggcatca atccggagcc ggctccgccg ggtcaggaga cccgggacga gggccagtag
     5641 cggcttccgc cccgggagct tcttcctcat cggcgaaaac ctcctccatc ttgcccgaag
     5701 gcgaaccgat aagggcgacc aggaagcaga ccgccatcac cccggccatg cagaggaaca
     5761 ccgtcttgct cgactgggcg aaagcgtcct ggattgcgtg gtagatcttc tcggacccgg
     5821 agctgccgcc cgatccgctc atgccgccac ttccggcgct ggacacctgg tcggcaatcc
     5881 tgtgagcttc cgaggtgggg acgatcccgt tcagctcgga ctcgatgttg gccttgttac
     5941 ggagaatgag aatcgtgccg aggacggcca tgccaacgct ggcgccgtag ttcctcaccg
     6001 tctgggtgat gccggtcgcc tcgccgtaac tcgtgttggg cgccctgttg actgcgtcgg
     6061 tggcgaccgg gccgagcaga aaaccgaatc cggcaccggt caacacgatg aacggccact
     6121 gggcattcga tccaccgtcc agatcggtca gcttcatcgc ccacaggaag aagccgaccg
     6181 tggcgagaag gcagccgagc acgacggtcg ggcgagcccc cctcgagtcg aggatgcggc
     6241 ccccgaactg cgccgcagtc gcaaagccgg agaagaagat cagcaggtag agaccggctt
     6301 cggaggcctg ccagtcgagc gagagctggg cgtacatgct ggcgaagaag aacagcggca
     6361 cgaaggcgat cgaaagcagg aagagcagaa cattgtccac ggcgaaggat cggtccctga
     6421 agatcgcgac ccggatcagc ggatgttcct gcttcatctc gaaagccacg aaaccagcca
     6481 ggaccaacag tccgccgact atgcatatcc aggtgactgg attgccccag ccccagatcg
     6541 acgactgctg caggccgaga accgccagcg ccatgccaat gcagaccagg acagcgccgc
     6601 ggtagtcgat cggctggggg cggcgggtct cctccggctt cgagatgtag atgaagatca
     6661 gggagaggat tgccaccggg atgttgatcc agaagatcgc gcgccaggtc cactccgaga
     6721 gatacccgcc ggccagcggg ccgatcgnnn nnnnnnnctc gacgcgccgc ccttgtattc
     6781 ctgcatccac atcgaggtga aggcaccggt caccccgaag aagatcgcca tagccttgcc
     6841 gcggtcggcg atcgggaagg aagagatcac gatcgccagc gcggccggga acatcatggc
     6901 ggcgcccgcg ccctggacca cgcggaagaa gatcagccag gtctcgtcga tgtttgtgtc
     6961 aggcgtgaag ccgcagaacg cggatgagac ggcgaagatc agcacgccga tcacgaccag
     7021 ccggcgatgg ccgaagacgt cggccagctt gccgccaagg gcgaaaaggg ccgcgagtga
     7081 gagcagatag ccgttgatca cccactggac cccggtcgag gtgaggctca aatcctcctg
     7141 gatcctgggg atcgagatcg agacgatcgt ctggtcgatg aaggtcatcg acacggcgaa
     7201 gatcatggcg accagaacga ggtaaccgct cttcttttct gccatctcgc cggaatttac
     7261 cgaagggccc ggcaactcaa acaaagttga ctcgcatcag cttccagcgg gtaggctgcc
     7321 agcatggaga tcctgcgcac gcccgaagaa aggttcgaat cgattccgga cttcccctgg
     7381 gagccggagt accgggagtg ggaaggcatg cgcctcgccc acatcgacga gggcgaggga
     7441 gaaccggtgc tgctggtcca cggcgagccg acctggggat acctgtggcg caaggtcatg
     7501 ggccctctgc tcgacgccgg ataccgctgc atcgttcccg acctgccggg attcggccgc
     7561 tccgacaaac ccgtagatga cgactggtac acctacgacc gtcacacagc cgcactggtc
     7621 agcctgatcg aagaactgga tctggacggc ctgaccctgg tctgccacga ctggggcggc
     7681 ccgatcgggc tgcgtgccgc ctcgatcgag gttcccgaac gcttcacgcg gcttgtcgcc
     7741 atggacaccg gactgttcac cggctaccag aagatgtcgg acaactggaa ccacttccgc
     7801 gacttcattg cctcccaccg tgacgtgcgg gtcgacatgc cgattcgcgg cggttgcgcg
     7861 accgaactct ccgacgaggt ggtggccgcc tacgaggccc cgttcccgga tgtcaactac
     7921 aaggcaggca tccgcacctt ccctccgatg atccccgtta cgcccgagga cccgggagca
     7981 gaagcaggtc aggaggcggc caagtacctg atcgccgacg atcgcccctc cctcgtcctg
     8041 tgggcggact cggacccggc cctccctatg gatccggtcg gcaagaccgt ccagatgctc
     8101 ttcccgaagg ccgagccact gaccgtgatc gagaacgccg ggcatttcct tcaggaggac
     8161 cacggcgagc agatcgggcg gataatcgcc gactggctca ggaagaactg agaagcattc
     8221 gcagcagcgg ctcggcccgg cgcgcgtcgg gctcctcgcc ggtgatcagg tccaggtata
     8281 tgtagccgcc catgacccgc tcgatcacgt aggcgagctc atacggctct acgccctccg
     8341 gcaggtccat gttcccggcc gccagctgcc tttcgatcga atcctcgacc caggcgatca
     8401 gccggggctg atagccggaa ccggccctgg tcagcagcga catcgccgcc tcgccctcgg
     8461 tcgcaagcca gtgcttcatc cccggattgc cgatcacctc ttccgagaat tcgatcgtca
     8521 catcgatgat ccggtccggg ccggtcgaat cgcattcacg atcgagccgg tccaggctgg
     8581 ccgaggtcat gtgccagatc atctcgacca ggaactgctc cctggaaccg acccagcgat
     8641 agagcgtggc ccggttgatg tcgagctgtc gggccatcgc cgacatgtcg atccgctcct
     8701 tcgccatgaa gttgctgatt ccgagccgca acgcacgcag cggccccgaa tcctcgggct
     8761 cacccggatc ggtcagcgca gcctgaagct ttgtggctcg cggagtcaca ggacgggcag
     8821 catttcgcat ttggacggat tccgcccgat cggaccgccc ccggtgaccg gttcccgccg
     8881 gcccgggacc cgcaagactc gagccggagg ggaatccggc cccgatccgg cccggaccgg
     8941 tcgtcgcccc tggcacgcca taggatgcgc ccatgtccaa cgaagacaag gggagcgcac
     9001 cggccaaacc ggcgttggta ctgaccagcc tgattcttgt cgccgcggtg gcgaacctga
     9061 atctggcggt cgccaatgtc gcccttcctg acatcggcaa ggacctcgat gccagccaga
     9121 ccggactgaa cctggttgcg gtcggcttct cgctcggcct tgcttcctcg gtgctctacc
     9181 tgggagcact cggagaccgc tacggccgca agatgctgct gatcatcggc acgacggttt
     9241 cgattccggc cgccctgctc gccggtttcg ccccttcggt cggcgtgctc ttcgtcgcca
     9301 gggtgatcgg gg
//
LOCUS       DAOTIP010000005         9041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_9184_length_9041_cov_3.787336, whole genome shotgun sequence.
ACCESSION   DAOTIP010000005 DAOTIP010000000
VERSION     DAOTIP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_9041_cov_3.787336"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1757
                     /locus_tag="P5138_00220"
     CDS             237..1757
                     /locus_tag="P5138_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59030.1"
                     /translation="MLAISKRGLLLPALLIGLISLVGLAVMSPRADAARSFNTAIVDA
                     DAFEHGSDAAFDHAAASGATIIRNNLYWAYVLADGESTTKPGTELQPFDPTDPASPYY
                     HWATFDKIVRKSAARGISVVFAVVSPPRWARISSCQGSGVCSPKPSDYADFVTAAARR
                     YSGTFDPGDGDGVLPRVRFWQAWVEPNLSLFYKPIFKGNGAPSAPYTFRNLLNAFYDA
                     VHSVNNSNFVIAGGLAPNAVPGKAIAPLDFTRRALCMTGSYKRPKPIRGCNFKVKADA
                     WAVHPYTTGSPVHSPSKPDNMTVSALPRMTNLLRKATQAKRLTSRFGRTQLWVTEFSW
                     DSKPSDPGGVPSKLLSRWVAQAMYLAYKANVQTFTWFGLRDQARSSGQKWSDTFESGL
                     YFRRASVDKDKAKPVLKAFRQPFYAEKTRAGFRFWGRTADSQKATVDIFGRRKGSGRF
                     SRVTTVQANANGLFAGVVRRRGFTARGSVFAKPRGASSSIAFGLWKTRDFYQPPFG"
     gene            complement(1765..2313)
                     /gene="rfbC"
                     /locus_tag="P5138_00225"
     CDS             complement(1765..2313)
                     /gene="rfbC"
                     /locus_tag="P5138_00225"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRV59031.1"
                     /translation="MASRLKTGLDGLALIEPQVFGDDRGFFLETFSRDAWRELGVEAG
                     FVQHNHSRSVGGTLRGLHFQTSPGQAKLLRCPRGKIFDVAVDLRRDSPTYGKWEGYVL
                     DDEKHHQLYVPVGFAHGFCVLSDVADVTYLVSSLYDPETEAGIRWNDPGIGVQWPVEE
                     PLLSQRDVEAPLLDEVADSLPW"
     gene            complement(2315..3307)
                     /gene="rfbB"
                     /locus_tag="P5138_00230"
     CDS             complement(2315..3307)
                     /gene="rfbB"
                     /locus_tag="P5138_00230"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRV59032.1"
                     /translation="MKLLVTGGAGFIGSNFVHHMVESGRGDGLVVLDKLTYAGRLENL
                     AGLDIEFVEGDICDRDLVRSLVERVDAVVNFAAESHVDRSIESPGEFITTDVFGTFVL
                     LEAARDAGIRHLQISTDEVYGSIDEGSFTEASPIVPSSPYSASKAGGDLIVGAFQHTY
                     GADTLIARASNNYGPQQHPEKLIPLAVLNAIHGDPIPVYGDGSQVRNWLFVRDFCTAI
                     ERVLDQGEAGQVYNVGGPDELPNIEVVRTILELTGRDESLITHVGDRLGHDIRYSLSS
                     EKTEGLGWKAATGFREGIARTVDWYRENEEWWGPLRSGEYREYYQRQYGRVLTD"
     gene            complement(3304..4176)
                     /gene="rfbD"
                     /locus_tag="P5138_00235"
     CDS             complement(3304..4176)
                     /gene="rfbD"
                     /locus_tag="P5138_00235"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRV59033.1"
                     /translation="MKITVLGARGMLGHDVVLAAGNEGHEVHGYGHSEVDITDPEAVS
                     RRLRLDRPDAVVNCAAWTDVDGAEDEPGGAMAVNGIGAGIVAEAAAAVDAKVLYVSSD
                     YVFDGSKKEPYLETDQTGPISAYGTSKLAGEVASMNGNRRCFVVRSSWLFGIAGGNFV
                     DTMIRLGMTQKQVLVVRDQIGCPTYTWHLAYGLVRLLDSDAYGIHHMAAGGHCSWYDF
                     AKEIFRLSDMEVTTLSATTEMFNAKAPRPAWSVLESGLEHAITLPSWQDGLAAYLQQR
                     GSVEGGGSDEGAES"
     gene            complement(4176..5249)
                     /locus_tag="P5138_00240"
     CDS             complement(4176..5249)
                     /locus_tag="P5138_00240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRV59034.1"
                     /translation="MTEIKGLILSGGSGTRLRPITHTYAKQLVPVANKPVLFYGIEAL
                     VEAGVTDIGIIIAPHTGDEVREAVGDGSVFGAKVTYIEQAEPAGLAHAVLTAEDFLDG
                     SSFVMYLGDNLLRDGVPGLVERFRAETPDAMILLTRVEDPTAFGVAELDEGRVVHLVE
                     KPENPPSDMALVGVYLFSPAIFDAAKRLEPSERGELEITEAIQTLIDDGHEVRSEVVT
                     GWWKDTGQLADMLEANRLVLDDIVTRLDGTIEDSRVEGRVIVEEGAVLRNSVVRGPAV
                     IGRGAVVDGAYIGPYTSIGDGVRVLNSEIEHSILLSGSSVENLGARIEASLLGREVRV
                     TRSDSLPKTMRLLVGDRAEIEIV"
     gene            5271..6227
                     /locus_tag="P5138_00245"
     CDS             5271..6227
                     /locus_tag="P5138_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV59035.1"
                     /translation="MPLATPTVYIPTLEGGDRLATCLTALQAQTADFEVVVADNGPGE
                     GCAQLLGNRFPEVVRIGFGGRNLGFGSALNRAVSERGSGPLIFLNDDAVPNPDFVESL
                     IDTRRESSAAMVAGVLLRADDPARIDSAGISCDRTLAAWDYLTGEPANRLDNAADPLG
                     PTGGAALFDREAFNQVGGFDERIFLYYEDLDLALRMRLAGYSCRLAPDARAIHEGSAT
                     LGRKAAAKYRQTGFSRGYLLRKYGIMRHPALALKVAVSDTLAGLAQLVADQTPAGLAG
                     RIDGWRSAGDLRLEAPLDQLEPASLRRHFQVRLARRNRDKPR"
     gene            complement(6224..6697)
                     /locus_tag="P5138_00250"
     CDS             complement(6224..6697)
                     /locus_tag="P5138_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV59036.1"
                     /translation="MGNKRSQIEMSEEEIQAFLEEERVAIVTSFGPRGWPHSMPLWYQ
                     VVDGRIRSWTFRKSQKVKNLERDPRATVLVEAGYDVYPELRGVMYEAETVIHDDSETV
                     LDYGLGSIGRYSPGGGEPDEAMIEAFRAQAAKRVVLEFVPVHTVTWDHRKLGGVY"
     gene            complement(6707..7444)
                     /locus_tag="P5138_00255"
     CDS             complement(6707..7444)
                     /locus_tag="P5138_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV59037.1"
                     /translation="MEEMADQAAPGHPEIFRVMADNPGPMTLTGTNTYLWGTDPCWVI
                     DPGPEDEAHIAAVKAAIEERGGAAGVLLTHSHADHSGGVESLGIEPVAITDGSVNGGL
                     ESVATPGHSVDHFCFFTEDGVCFSGDLVLGWGSTYVPPDGGSLAAYMLSLRLIQSRAP
                     SLICPGHGPWINDAAGKITEYIDHREARENDLLEALSRGERSRMNLLEEVWSDVPEQL
                     RPAANVVMEAHLQKLEAEGRLPDDLAE"
     gene            complement(7448..>9041)
                     /locus_tag="P5138_00260"
     CDS             complement(7448..>9041)
                     /locus_tag="P5138_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRV59038.1"
                     /translation="LTQRLARIETAISRHKTITFEYHTIGSDKVSDRKVDPYHLVFRG
                     GQFYLIGHSHERDAVRVFRLSRIRGKVGYATKSEHDFNAPEDFERRDYAELADWQLGE
                     ETGRARIFFSDRIAWLIERDFGSHGEVSPADPKETGGREGIVLETSYASERELLAWVL
                     RWRQNAEVLGPPEFKAAAEERIALLRDRHINGFDVAEAQTDFPTPIRERRQRSKDRSA
                     AAIRPERFGRLVTLAGMLIGAAKEGTALSMSDLCQRLELSEEELRDDIDVLNVVNFGG
                     GTYVLYAEIKGDIIEVDSEPYGDSFARPARLLPLEAKALVAAIDLVGDHLPQSKLDTA
                     REKIIEALGRDPAHEGLAIVSESGGAEAARIVNEAIAKRQVLELNYYKENEDKFSERK
                     VEPYRLENGREGWYAECFDLDRQAMRHFKLDRIKDANPTGENFEPRPEAEELAGVEGW
                     MSDGQVPEASVARVWVSPERSRWVREERNVIEELADGAVVVELPYAGTSWLVREILRG
                     AGDLVVLEPQEARDAISRELAA"
BASE COUNT         1558 a         3026 c         2855 g         1602 t
ORIGIN      
        1 tcaggttcgg gctctactac gacaccggcc gtctccagga tgcccggcgt gacctcgggc
       61 ggctgcggga gaccaacccg gtcttgttca gccgactcac tttcagggaa gtcaacgaac
      121 tggccaagtg acccgcaggc ttccggttcg ctactttctg cccccggcga tgttcaaacc
      181 aatacgcgcc cgggacttcg cgcccttgag cgaagtctga actccgaaag gaatccttgc
      241 tcgcaatttc gaaacgtgga ctgctgctgc cggcactgct gatcggcctg atcagcctgg
      301 tcggccttgc cgtcatgtct ccgcgtgccg acgcggcgcg ttccttcaac acggccatcg
      361 ttgacgcgga tgccttcgaa cacggatccg acgcggcatt cgaccatgcg gcggccagcg
      421 gggccacgat catcaggaac aacctttact gggcgtatgt tcttgccgac ggcgaatcga
      481 ccaccaaacc gggaacggaa ctccagcctt ttgacccgac cgacccggcc agcccttatt
      541 accactgggc aaccttcgac aagatcgtcc gcaagtcggc ggcacgcgga atcagcgtcg
      601 ttttcgccgt tgtgtcacca ccgcggtggg ccaggatctc cagctgccag ggttccgggg
      661 tctgctcacc gaagccatcg gactatgcgg atttcgtcac cgcggcagcc cggcgctaca
      721 gcggcacctt cgacccggga gacggtgatg gtgtcctgcc cagggtgcgt ttctggcagg
      781 catgggtcga gccgaatctg tccctgttct acaagccgat cttcaagggc aacggtgctc
      841 cctccgcgcc ctacaccttc cgcaacctcc tcaacgcttt ctatgacgcg gttcactcgg
      901 tcaacaactc gaacttcgtg atcgccgggg gactggctcc caatgcggtc cccgggaagg
      961 cgattgcccc gctcgatttc acccgccggg cgctctgcat gaccggcagc tacaagcggc
     1021 ccaagccgat caggggatgc aacttcaagg tcaaggcgga tgcctgggcc gtccatccgt
     1081 acacgaccgg ctcgccggtc cattcaccct cgaagccgga caacatgacc gtctcggccc
     1141 tgccgcgcat gacgaacctt ctgcggaagg ccacgcaggc gaaacggttg accagccggt
     1201 tcggccgcac ccagctctgg gtgaccgagt tcagctggga ttcaaagccg tcggacccgg
     1261 ggggagttcc gtcgaagctg ctttcgcgct gggtcgcgca ggccatgtat ctggcctaca
     1321 aggcgaacgt ccagaccttc acctggttcg gactccgcga ccaggcacgc tcatccggac
     1381 agaagtggag cgacaccttc gaatcgggcc tgtacttccg ccgggcatcg gtggacaagg
     1441 acaaggcgaa gcccgtcctg aaggcattcc gccagccctt ctacgccgag aagaccaggg
     1501 cgggattccg gttctgggga cgcaccgcag acagccagaa ggcgaccgtg gacatcttcg
     1561 gacgccgtaa gggcagcggt cggttctcca gggtgaccac ggtgcaggcg aacgccaacg
     1621 ggctcttcgc cggagtagtc cgcaggcgcg gattcaccgc acgcgggtcc gtcttcgcga
     1681 agccacgggg ggcatcctca tcaatcgcct tcggcctctg gaagaccagg gacttctacc
     1741 agccgccatt cggctgatac cgccctacca ggggagcgag tcggcgactt cgtccagaag
     1801 cggcgcctcg acgtcgcgct gcgacagcag cggttcctcc accggccact gaaccccgat
     1861 cccggggtcg ttccagcgga ttccggcctc ggtttccggg tcgtagaggc tggaaactag
     1921 ataggtgacg tcagctacgt cggagagcac gcagaacccg tgggcgaagc cgaccgggac
     1981 atagagctgg tgatgcttct cgtcgtccag cacatacccc tcccacttgc cgtaggtagg
     2041 ggaatcgcgg cgcaggtcga cggcgacatc gaagatcttc ccgcgggggc agcgcagcag
     2101 tttggcctgg ccgggagatg tctggaaatg aaggccgcgc agggtcccgc cgaccgacct
     2161 cgagtggttg tgctgcacga acccggcttc cactccgagc tcgcgccagg cgtcacggct
     2221 gaaggtctcg aggaagaagc cccggtcgtc cccgaatact tgcggttcga tcagcgcaag
     2281 accgtcgagg cccgttttga ggcggctggc catctcagtc ggtcaggacc cggccgtatt
     2341 gacgctggta gtactcgcgg tactcacctg agcggagtgg cccccaccac tcctcgtttt
     2401 cgcggtacca gtcgacggtg cgggcgatcc cttcacggaa cccggttgcc gccttccagc
     2461 caagcccctc ggttttctcc gaggagagcg agtagcggat gtcgtggccg agccggtcgc
     2521 cgacgtgggt gatcagcgat tcgtcccgtc cggtgagttc gagaatggtg cggaccacct
     2581 cgatgttcgg cagctcgtcc gggccgccca cgttgtacac ctgccccgcc tcgccctggt
     2641 cgaggacccg ttcgattgcg gtacagaagt cacgcacgaa aagccagttg cggacctggc
     2701 tcccgtcgcc gtagaccggg atcggatcgc cgtgaatcgc gttcagcaca gccagcggaa
     2761 tcaacttctc cggatgctgc tgcgggccgt agttgttgga ggcccgggcg atcagcgtgt
     2821 cggctccgta agtgtgctga aaggccccga cgatcagatc accgccggcc ttcgaggccg
     2881 aataggggga tgagggaacg attggggagg cttcggtgaa cgaaccctcg tcgatcgagc
     2941 cgtagacctc gtcggtcgag atctggagat gacggattcc ggcgtcgcgg gccgcctcga
     3001 gcagaacgaa cgtgccgaat acgtcggtgg tgatgaactc gccgggagac tcgatcgacc
     3061 ggtcgacgtg ggattcggcc gcgaagttga ccacggcatc gacccgctcg acgaggctgc
     3121 gaaccaggtc ccggtcgcag atgtcacctt ccacgaactc gatatcgagc cccgcgaggt
     3181 tctcgagtcg ccccgcgtag gtgagcttgt ccagcaccac gagaccgtcg ccccggccgg
     3241 actcgaccat gtgatggacg aagttcgaac cgatgaagcc ggccccgccg gtaacgagga
     3301 gtttcacgac tccgctccct catcgctgcc gccgccctcg acgctgcccc gctgctgcag
     3361 gtaggcggcg agaccatcct gccaggaggg cagggtgatc gcatgctcga ggccgctctc
     3421 gagcaccgac caggccggcc gcggcgcctt ggcgttgaac atctcggtgg tggcggagag
     3481 ggtcgtcact tccatgtcgg acagtctgaa gatttccttg gcgaagtcgt accaggagca
     3541 atgcccgccg gcggccatgt ggtggattcc gtaggcgtcg gaatcgagca ggcggaccag
     3601 gccgtaggcg aggtgccagg tataggtcgg gcagccgatc tggtcccgga ccaccagcac
     3661 ctgcttctgg gtcatgccga gccggatcat cgtgtcgacg aagttgccgc cggcgattcc
     3721 gaatagccag gaagagcgaa caacgaagca gcgccggttg ccgttcatgc tcgcgacctc
     3781 gccggcgagc ttcgaggtgc cataggccga gatcgggccc gtctggtcgg tttccagata
     3841 gggctccttc ttcgaaccgt cgaagacgta atcgctcgag acgtacagga ccttggcgtc
     3901 gacggcggct gcggcttcgg cgacgatccc ggctccgatg ccgttcaccg ccattgctcc
     3961 ccccggctca tcctcggcac catcgacgtc ggtccaggcg gcacagttaa cgaccgcatc
     4021 cggacggtcc agtcgaagcc tgcggctgac cgcttcggga tcggtgatgt ccacctcaga
     4081 gtggccgtag ccgtgaacct cgtggccttc gtttccggct gccagcacga cgtcgtgccc
     4141 cagcattccg cgggctccga gcacggtgat cttcatcaga cgatctcgat ctcggcgcga
     4201 tcgccaacca ggagccgcat cgtcttcggc agggagtcac tacgggtgac ccgcacctcg
     4261 cgacccagca ggctggcttc gatccgggct cccaggttct ccaccgagga accggacagg
     4321 aggatcgagt gctcgatctc cgagttgaga acccggaccc cgtcaccgat cgaggtgtag
     4381 gggccgatgt aggcgccatc gacgaccgct cccctgccga tcacggcggg accccgcacg
     4441 accgagttgc gcagcacagc tccttcctcg acgatcaccc ggccctcgac ccgcgaatcc
     4501 tcgatggtcc cgtccagccg ggtaacgatg tcgtcgagga cgagccggtt cgcctccagc
     4561 atgtctgcca gctggccggt gtccttccac cagccggtca ccacctcgga gcgcacctcg
     4621 tggccgtcgt cgatcagggt ctggatcgcc tcggtgatct cgagttcacc gcgttcggaa
     4681 ggttcaagcc ttttcgccgc gtcgaagatc gccggcgaga acagatacac gccgaccagg
     4741 gccatgtccg acggcgggtt ctccggcttc tcgaccaggt ggaccaccct tccctcgtcg
     4801 agttcggcca cgccgaaggc ggtggggtcc tcgacccggg tcaggaggat catcgcgtcg
     4861 ggagtctccg cccggaaccg ctcgaccagt cccggcaccc cgtcccggag caggttgtca
     4921 ccgaggtaca tgacgaagga agatccgtcg aggaagtcct cggcggtgag cacggcgtgg
     4981 gcgagcccgg ccggctcggc ctgttcgatg taggtgactt tcgccccgaa aacggatccg
     5041 tcgccgaccg cttcccgcac ctcgtcaccc gtatgcggag caatgatgat gccgatgtcg
     5101 gtgacgccgg cttcgaccag cgcctcgatc ccgtagaaga gcacgggctt gttggccacg
     5161 gggaccagtt gcttggcgta ggtgtgggtg atcgggcgca gccgcgttcc ggaaccgccc
     5221 gagaggatca gacccttgat ctcggtcatg aatggtcaag aatatcctga atgcctctgg
     5281 ccaccccgac tgtgtacatc ccgaccctgg aaggcggcga ccgcctagcc acctgcctga
     5341 ccgcccttca agcccagacg gccgattttg aggtggtagt ggccgacaac ggccccggtg
     5401 aagggtgcgc tcaattgctc ggcaaccggt tccctgaagt ggtcagaatc ggctttggag
     5461 ggcggaatct gggattcgga tccgcattga atcgtgcggt ctccgagcgt ggctccggcc
     5521 cgctcatctt tctgaatgac gacgccgttc ccaatcccga tttcgtcgaa agcctgattg
     5581 atacccggcg ggagagcagc gcagcgatgg ttgccggggt cctgctcagg gccgacgacc
     5641 cggcccgaat cgattcggcc ggcatttcct gcgaccgaac cctggccgca tgggattacc
     5701 tgaccggcga accggcaaac cgtctggaca acgccgccga cccgctcgga ccgaccgggg
     5761 gcgcggcact cttcgaccgt gaagctttca atcaggtcgg tggctttgac gagcggatat
     5821 tcctctacta cgaagatctc gacctcgccc tcaggatgcg cctggccggc tactcctgcc
     5881 gccttgcgcc agatgcccgc gcgatccacg aagggtcggc cacactcggc cgcaaggccg
     5941 ctgccaagta ccgccagacc gggttcagcc ggggctacct gctgcggaaa tacggaatca
     6001 tgcgccaccc cgctctggcc ctgaaggtag ccgtcagcga cactctggca ggactcgccc
     6061 agctggtcgc cgaccagacg ccagccggac tggcgggaag aatcgacggc tggcggtcag
     6121 ccggtgacct gcgactcgaa gcaccactcg accaactcga accggccagc ctccgccgcc
     6181 actttcaggt ccgcctggcc cgccggaatc gcgacaagcc gcgctagtac accccaccga
     6241 gcttgcggtg atcccaggtc acggtgtgca ccggaacgaa ctcgagcacc acccgtttgg
     6301 ccgcctgggc acgaaaggcc tcgatcatcg cctcgtccgg ttcaccaccg cccggcgagt
     6361 agcgcccgat cgaaccgagc ccgtaatcga gcacggtttc ggaatcatcg tgaatcacgg
     6421 tctcggcttc gtacatgacg ccgcgcagtt ccggataaac gtcatagccc gcttccacca
     6481 ggaccgtcgc ccggggatcc cgttcgaggt tcttgacctt ctgtgacttg cggaaggtcc
     6541 aggagcggat cctgccgtcg accacctggt accagagcgg catcgagtga ggccagcccc
     6601 gcggcccgaa gctggtgacg atcgcgaccc tctcttcttc gagaaaggcc tggatctctt
     6661 cctcgctcat ttcaatctga ctgcgcttgt tgcccatgcc gggaccctac tccgcaaggt
     6721 cgtccggcag gcggccttcg gcttccagct tctggagatg cgcctccatg accacattgg
     6781 cggccggcct caactgttcc ggcacatccg accagacttc ttcgagcaga ttcatccgtg
     6841 accgctcgcc tcgggacaag gcttcgagca gatcgttctc gcgggcctcg cggtggtcga
     6901 tgtactccgt gatcttgccc gcggcatcgt tgatccaggg accgtggccc gggcagatca
     6961 acgaaggggc ccgggattga attagtcgca gcgaaagcat gtaggcggcc agcgaaccgc
     7021 cgtccggcgg gacataggtg gaaccccagc cgaggaccag gtcgccggag aaacagaccc
     7081 cgtcttccgt gaagaagcag aagtgatcaa ctgaatggcc gggagtcgca accgattcga
     7141 ggccgccgtt caccgaaccg tcagtgattg cgacgggctc gatccccagc gattccacac
     7201 cgccactgtg gtcggcgtgg gaatgcgtga gcagcacgcc cgcagccccg ccgcgctctt
     7261 cgatcgccgc cttcaccgcc gcgatgtgag cctcgtcttc cggaccggga tcgatcaccc
     7321 agcagggatc ggtgccccac agataggtgt tggtcccggt cagggtcatc ggaccggggt
     7381 tgtcggccat cacccgaaag atctcgggat gcccgggagc ggcctggtcg gccatctcct
     7441 ccatgggtca ggcagctagt tcgcggctga ttgcgtcgcg ggcttcctgg ggttccagga
     7501 cgacgaggtc accggcgccg cggaggatct cccggaccag ccagctggtt ccggcgtacg
     7561 gaagctcgac cactaccgcg ccatcggcga gttcctcgat gacatttctt tcctcgcgga
     7621 cccagcgcga acgctccggt gagacccaga cccgggcgac cgaggcctcc ggcacctggc
     7681 cgtcgctcat ccagccttcg accccggcca gctcttcggc ttcgggccgc ggctcgaagt
     7741 tttcgccggt cggattcgcg tccttgatcc ggtcgagctt gaagtggcgc atcgcctgac
     7801 ggtcgagatc gaaacactcg gcgtaccagc cttcgcgtcc gttctccaga cggtacggct
     7861 cgaccttgcg ttcggagaac ttgtcctcgt tctccttgta gtagttgagt tcgagaacct
     7921 gccgtttggc gatcgcctcg ttgacgatcc gggccgcttc cgcgccgccc gactcggaaa
     7981 ctattgccag cccctcatgg gcgggatccc ggcccagggc ctcgatgatc ttctcccggg
     8041 cggtatccag cttggactgc ggcaggtggt cgccgaccag gtcgatcgcc gcgaccagag
     8101 ccttggcttc gagcggcagc aggcgggccg gacgggcgaa ggaatcaccg tagggctcgg
     8161 aatcaacctc gatgatgtcg cccttgatct cggcgtagag cacgtaggtg ccgccgccga
     8221 agttgacgac gttgagcacg tcgatgtcat cccgcaactc ttcctcggaa agctcgagcc
     8281 gctggcagag atccgacatc gacagcgccg tgccttcctt cgcggcaccg atcagcatgc
     8341 cggcgagagt gaccaggcgc ccgaagcgct cgggccggat cgccgcggcg gaacggtcct
     8401 tgctgcgctg gcgacgttcc ctgatcgggg tcgggaagtc ggtctgggcc tcggccacgt
     8461 cgaagccgtt gatgtggcgg tcccggagca gggcgatcct ctcctctgcc gccgccttga
     8521 actcgggggg accgagcact tcggcgttct ggcgccagcg gagcacccag gccaggagct
     8581 cccgttcgct ggcgtaggag gtttccagca cgatgccttc cctgcccccg gtctccttcg
     8641 ggtcggccgg gctgacctcg ccgtggctgc cgaaatcccg ttcgatcagc caggcgatgc
     8701 ggtcgctgaa gaagatccgg gcccggccgg tctcctcgcc gagctgccag tcggccagct
     8761 ccgcgtagtc gcgccgctcg aaatcctcgg gcgcgttgaa atcgtgctcg gacttggtcg
     8821 cgtaacccac cttgccgcgg attcgggaga gccggaagac ccggaccgca tcgcgttcat
     8881 gggagtggcc gatcaggtag aactgcccgc cccggaagac caggtggtag ggatcgacct
     8941 tgcggtcgga gaccttgtcc gaaccgatcg tgtggtactc gaaggtgatc gtcttgtgcc
     9001 gcgagatcgc ggtctcgatg cgggccagac gctgggtcag g
//
LOCUS       DAOTIP010000006         8793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_9545_length_8793_cov_3.683566, whole genome shotgun sequence.
ACCESSION   DAOTIP010000006 DAOTIP010000000
VERSION     DAOTIP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9545_length_8793_cov_3.683566"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..580
                     /locus_tag="P5138_00265"
     CDS             176..580
                     /locus_tag="P5138_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRV59039.1"
                     /translation="MSDEEATEIQPYERNSAEVAELIAGGVRLIDVRTAHEHEAARLA
                     DSERIGLEELLDRRDEIGKDETVVFYCRVGNRSGMAAQAFSEAGYDTLNLYGGIVQWI
                     EDGQPIEPADGYVAEPGEAAAILEAQARAAGN"
     gene            903..2801
                     /locus_tag="P5138_00270"
     CDS             903..2801
                     /locus_tag="P5138_00270"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRV59040.1"
                     /translation="MEPTSAPSQPKPAAKPKPKPGDKGVEQEEGKLQTGKGKLGTLTN
                     PETYDNVPGHIIPILEHEFDDFDAEADEFLAGNTPEPEFIGFRLKQGVYGQRQPGVQM
                     VRVKLPYGGITPEQMDAFADAVETYAPLNKGHITTRQNIQIHHVPLDQMKHMLRDLAP
                     SGLSSREGCGNTVRNVTGDPWAGVAEDEIFDPTTYVGAYVRYFVRHPTTQLMPRKIKT
                     AFTGSDADRAITGIHDIAFISRIRDGVRGFEVRVGGGTSIMARVAPTLYDFVEADNGD
                     FLKVSEAVFRIFDRQSWLRVNRARARIKVLVDKVGIEEVRRMVDEELKGDWVDERDFT
                     VDHMLFDLDEESNLTPAVISSDSPNGDRSAFDAFVAANVEPQKQAGFNTVEIKITRGD
                     LTPEQFRGLGQIMRDHSGGFARTTVQQNLVLRWVRDESLYDVYRALGELGLDEPGPRT
                     ITDVVSCPGTDSCKLGITSSMGLNRAIQEKVTGMNITDPLTRQIHIKMSGCPNGCSQH
                     HIANIGFYGASLKVSGKTMPAYVPHLGGKYVDGEVIYGTRLKSRLPARRVPEAVERWI
                     NLYEADRNEGEVFNDFVERVGTARMEEEVKELTMPAEFSLESMQQFIDWNRVDPYKVE
                     RGEGECAI"
     gene            2808..3563
                     /locus_tag="P5138_00275"
     CDS             2808..3563
                     /locus_tag="P5138_00275"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRV59041.1"
                     /translation="MEPSSTVSPAEAEAVRKAEEATRALAARFDGESEAARLEPLDAS
                     GILEWAFEQFGRDMYIACSFQKTSSVVMHLATEINPDARFFYIDTDFLFPETYETRDR
                     LAEHFGVEFERWNNISPEEQEREFGDHLWKRDPDACCGIRKVDPMRRALSSGVDCWVA
                     GIRREDSATRAGSPKFMWDRKFKIWKLNPIADWTERDVWTYLHKHGIPYNPLHDQGYP
                     SIGCTHCTLPVHPGQSSRDGRWSGSGKTECGIN"
     gene            3660..4727
                     /gene="sat"
                     /locus_tag="P5138_00280"
     CDS             3660..4727
                     /gene="sat"
                     /locus_tag="P5138_00280"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRV59042.1"
                     /translation="MTELALQLSERQTADFEMIAIGAFAPLTGFQGHADWQRICDEMR
                     TESGEYWPIPITLPTDLDCQIGDRIVLSADNGKHLGSITVEEIFERDVREEAQKVYRT
                     VDENHPGVAAIYEEGSRCIAGPIEVDALPDHEEAFMRRYLTPAESKQAFADRGWKKIV
                     AFQTRNPIHRAHEYLTKAALEACDGLFIHPLIGKTKAGDIPADVRMKCYELLMENYYP
                     NDRVMLGVKPAKMHYAGPREAVLHAIIRRNYGCTHFIVGRDHAGAGDYYGTFEAQEIF
                     DDIDINELGIEPLMFEHTFWCHKCEGLASNKTCPHDAESHLFLSGTKVREMLGNGEMP
                     PPEFSRPEVAQILIDSYKEDN"
     gene            4731..5282
                     /gene="cysC"
                     /locus_tag="P5138_00285"
     CDS             4731..5282
                     /gene="cysC"
                     /locus_tag="P5138_00285"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRV59043.1"
                     /translation="MSDSKGFTLWFTGLSGAGKTTISEIVARELTARGSKLEILDGDI
                     VRENLSKGLGFSKEDRDTNIRRIAFVADLLSRNGVPVITAAISPYRAIRDEARDLMGD
                     RFIEVYIEASVEVCEARDVKGLYAKARSGEIPEFTGVSDPYEAPENPEIALKTAEEEP
                     EESAARLITYLEERQLIPAAVAS"
     gene            5421..5894
                     /locus_tag="P5138_00290"
     CDS             5421..5894
                     /locus_tag="P5138_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRV59044.1"
                     /translation="MSVSLNIQRSGAPEGHAPQRVPLVDEDGLVTIDPWWGTIQPQRL
                     HPDIPTWGELEVIEHIEAGGRLIDTRLPEYVEASGTIPGSLVLPWETITDHQEVIDSE
                     SVTVLFCNGPQCAATPQAVGRLLDAGVEPEALAYYRGGIQDWIGLGLPTVPAPPD"
     gene            complement(6016..6822)
                     /locus_tag="P5138_00295"
     CDS             complement(6016..6822)
                     /locus_tag="P5138_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV59045.1"
                     /translation="MIELQSHSVISDGQLEPAGVVEEAAKAGVEVLALTDHDGVAGVP
                     EAIEAGERLGVEIVPAVEMSSVHEYAEDLHICGYWVDVEKITPACERAQQERRNRAKE
                     IVENLRSYGFDLTFEDAVREAGAADAIGRPHIAKAAGATGNLGPFFEEWLVPGAKAFV
                     SRKWPDANEACALIREAGGVPVIAHPYWDIKVPEQVEELIRALDIGGIEVFYPSHTRA
                     QTKHLLDLANELGVTATASSDYHGPTHKTFSRFGAYQTYDLGEPAVPPKP"
     gene            7039..>8793
                     /locus_tag="P5138_00300"
     CDS             7039..>8793
                     /locus_tag="P5138_00300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRV59046.1"
                     /translation="MFSKILIANRGEIAIRVARACKEMGITSVAVYSEADRDAPHVKA
                     CDEAFLIGPAVPAESYLSIEKIIQACRDSGAEAVHPGYGFLAENAEFARALDEAGINF
                     IGPPASAIDAMGSKTMAREVMEKAGVPIVPGATEPAKDLDEAKAQAEAAGYPVACKAV
                     GGGGGKGFRVAMSPDELQEAFEGSAREGEKFFSDDRVYVERYLEDPRHVEVQVLADKH
                     GNVIHLGERDCSIQRRHQKVIEEAPGPHVDEEMRERIGKIATDAARAVGYFSAGTIEG
                     MQHGDEYFFLEMNTRVQVEHCVTEMITGVDIVREQIKIAYGEELSISQEDVEIDGWAI
                     ECRINAEKADKNFAPSPGPIDVYNEPSGPGVRVDSGVKAGSEVTPMYDPMVAKLIVWD
                     KDRESATKRMLRALDEYEIAPLSTLIPFHKTILRTEQWARGETCRELMEDKKWLKTTA
                     PEDNSIPEPAEGEAEKVARDYLVEVSGKRFDVKVIGEATGFAAGAAPAGGGPKPKREK
                     KAGGGGGGGGPVLDSPLQGSIFKLEVEEGAEVAEGDLICVIEAMKMENEITAHKGGKV
                     TKVSISVGDAVASGDPLVT"
BASE COUNT         1677 a         2935 c         2762 g         1419 t
ORIGIN      
        1 ccagcaggcc ctgaagcagg cccaggattc gctccgcaac atccgcgccg tggacatcgt
       61 ctcgaccggc atccgcggcg agaacctcga cgagtaccgc gcccacgtcc gggtcgcgtt
      121 cctgatcgaa ggcaaagacg taacgtgagt tcggggttgc tacctttctc gacccatgtc
      181 tgacgaagaa gcaaccgaaa tccagcctta cgaacgcaac tccgccgagg tggcggagtt
      241 gatcgccggc ggcgtccgac tgatcgatgt gcggaccgcc cacgagcatg aggcggcccg
      301 cctcgccgac tccgagcgga tcggcctcga agaactgctc gaccgccgcg acgaaatcgg
      361 caaggacgag accgtcgtct tctactgccg ggtcggcaac cgctcgggca tggccgccca
      421 ggccttctcc gaggccggct acgacacgct caacctttac ggcgggatcg tccagtggat
      481 cgaggacggc cagccgatcg agccggccga cggctacgtg gccgaaccgg gcgaggcggc
      541 cgcgatcctc gaagcccagg cccgggccgc aggaaactag cccggctttc cacgggcttc
      601 tgtgccggtt ccggcttcaa gtcctggaaa cccggagcat ccccgcggcc gcccgaaccg
      661 aggtcgagga accggagact cccggttggt ccggtgcggg tgtgcccgga aaagaccttc
      721 gggggagccc gcagggcgac gggcttttcc gggcgcaccc gtgccggacc ctgtcgccga
      781 ggctctggga gaaaaggctg ttgcgtggta atacctgaaa tccggtcagg ttttggtctt
      841 tttgctaccg ttctctgttc gaaccagatt tcaagccctt tccgtagcag gaggaaccgc
      901 aattggagcc cacttcggcc ccgtcccaac cgaagccagc cgccaagcca aagcccaaac
      961 cgggcgacaa gggagtcgag caggaagagg gcaagctcca gaccggcaaa ggcaagctgg
     1021 ggaccctgac caacccggag acctacgaca acgtcccggg tcacatcatc ccgatcctcg
     1081 agcatgagtt cgacgatttc gacgccgagg cggacgagtt cctggccggc aacaccccgg
     1141 agccggagtt catcggcttc cgcctgaagc agggcgtcta cggccagcgc cagcccggcg
     1201 tgcagatggt ccgtgtgaag ctcccctacg gcggcatcac cccggagcag atggatgcgt
     1261 tcgccgatgc ggtcgagacc tacgcgccgc tcaacaaggg ccacatcacg acccggcaga
     1321 acatccagat ccatcacgtt ccgctggacc agatgaagca catgcttcgg gacctcgcgc
     1381 cgagcggact ttcttcacgc gaaggctgcg gcaacacggt tcgcaacgtg accggagacc
     1441 cgtgggccgg cgtcgccgag gacgaaatct tcgacccgac cacctacgtc ggcgcctacg
     1501 tgcggtactt cgtgcgccac cccacgacac agctgatgcc gcgcaagatc aagaccgcct
     1561 tcaccggctc cgacgccgac cgggcgatca ccggtattca cgacatcgcc ttcatcagcc
     1621 ggatccggga cggagtcagg ggtttcgagg tccgggtcgg tggcggcacc tcgatcatgg
     1681 cccgcgtcgc ccccaccctt tacgacttcg tcgaagccga caacggtgac ttcctcaagg
     1741 tctccgaggc cgtcttccgc atcttcgacc gccagtcctg gctccgggtc aaccgggccc
     1801 gggcccggat caaggtgctg gtcgacaagg tcgggatcga agaggtccgc cgcatggtcg
     1861 acgaggaact gaagggcgac tgggtggacg agcgcgattt caccgtcgac cacatgctct
     1921 tcgacctcga cgaggaatcc aacctgaccc cggctgtgat ctccagcgac tcaccgaacg
     1981 gtgaccgctc cgccttcgac gccttcgtcg ctgccaacgt cgaaccgcag aagcaggcgg
     2041 gcttcaacac cgtcgagatc aaaatcaccc gtggcgacct gaccccggaa cagttccgcg
     2101 ggctcggcca gatcatgcgt gaccattccg gcggcttcgc ccggaccacg gtccagcaga
     2161 acctggttct tcgctgggtc cgcgacgaaa gcctctatga cgtctaccgg gcactcgggg
     2221 aactcggcct ggacgagccc ggcccgcgga cgatcaccga cgtcgtctcc tgccccggga
     2281 ccgactcatg caagctcggc atcacctctt cgatgggcct gaaccgggcg atccaggaga
     2341 aggtgaccgg catgaacatc accgatccgc tgaccaggca gatccacatc aagatgagcg
     2401 gctgcccgaa cggctgcagc cagcaccaca tcgccaacat cggcttctac ggtgcctcgc
     2461 tgaaggtcag tggcaagacc atgccggcct acgtgcccca cctcggcgga aagtacgttg
     2521 acggggaagt gatctacggc acccggctga agtcccgtct gcccgcccgg cgggttccgg
     2581 aagccgtcga gcgctggatc aacctctatg aggccgaccg caacgagggc gaagtcttca
     2641 acgacttcgt cgaaagggtt ggcaccgccc gcatggaaga ggaagtgaag gaactcacca
     2701 tgccggccga gttcagcctc gagtccatgc agcagttcat cgactggaac cgggtcgacc
     2761 cgtacaaggt cgagcgcggt gaaggcgagt gcgcgatcta ggagcagatg gaaccttcct
     2821 ccacagtcag cccggcggaa gccgaagccg tgcggaaagc tgaagaggcc acccgggccc
     2881 tggctgcccg tttcgacggg gagtccgagg ctgcccgtct cgagccgctg gacgcgagcg
     2941 ggatcctcga gtgggccttc gagcagttcg gccgggacat gtacatcgcc tgctcgttcc
     3001 agaagacgag ttcggtggtg atgcatctgg cgaccgagat caacccggac gcccggttct
     3061 tctacatcga caccgacttc ctcttccccg agacttacga gacccgggac cgtctggccg
     3121 agcacttcgg agtcgaattc gagcgctgga acaacatctc ccccgaggag caggaacgcg
     3181 aattcggcga ccatctctgg aagcgcgacc ccgatgcctg ctgcggaatc cgcaaggttg
     3241 acccgatgcg ccgggcactc tcatcgggtg tcgattgctg ggtcgccggc atccgccgcg
     3301 aggattcggc gacccgggcc ggatcgccca agttcatgtg ggaccggaag ttcaagatct
     3361 ggaagctgaa cccgatcgcc gactggaccg aacgggatgt ctggacttat ctccacaagc
     3421 acggaattcc gtacaatccg ctgcacgatc agggctatcc ctcgatcggc tgcacccact
     3481 gcaccttgcc cgtccacccc ggtcaatcct cccgggacgg acgctggtca ggctccggca
     3541 agacggagtg cggcatcaac tagccgccga tcggacggca attgccgccc ataacggcta
     3601 ggctgctgga ttcttcacct ttcctgtcaa cattcccccc cacatccatg agcgtgagca
     3661 tgactgaact agccctccag ctttccgagc gacagactgc cgatttcgag atgatcgcga
     3721 tcggggcatt cgcccccctg accggcttcc agggacatgc cgactggcag cggatctgcg
     3781 acgagatgcg caccgagtcg ggggagtact ggccgatccc gatcaccctg ccgaccgacc
     3841 tcgattgcca gatcggcgac aggatcgtgc tcagcgccga caacggcaag cacctgggtt
     3901 cgatcacggt cgaggagatt ttcgaacgcg acgtgcgcga ggaagcgcag aaggtctacc
     3961 gcacggttga cgagaaccat ccgggcgtcg ccgcgatcta cgaagagggc agccgctgca
     4021 tcgccggtcc gatcgaggtc gatgcgctgc ccgaccacga agaagccttc atgcgccgtt
     4081 acctgacccc ggccgagtcg aagcaggcct tcgcggaccg tggctggaag aagatcgtcg
     4141 ccttccagac ccgcaacccg atccaccggg cccacgagta tctgaccaag gccgcgctcg
     4201 aagcctgcga cggcctgttc atccatcctc tgatcggcaa gaccaaggcc ggtgatatcc
     4261 cggccgacgt gcggatgaag tgttacgagc tgctgatgga gaactactac ccgaacgacc
     4321 gggtgatgct cggggtcaag ccggccaaga tgcattacgc ggggccgcgc gaagcggttc
     4381 ttcacgcgat catccgccgc aactacggct gcacccattt catcgtcggc cgcgaccacg
     4441 ccggtgccgg tgattactac ggcaccttcg aggcgcagga gatctttgac gacatcgaca
     4501 tcaatgagct cggcatcgag ccgctgatgt tcgaacacac cttctggtgc cacaagtgcg
     4561 agggcctggc ttcgaacaag acctgccccc atgacgccga gtcgcacctg ttcctttccg
     4621 gcaccaaggt gcgcgagatg ctcggaaacg gcgagatgcc gccgcccgag ttctcccgcc
     4681 ccgaggtcgc ccagatcttg atcgactctt acaaggagga caactaacca atgtcagaca
     4741 gcaagggctt cacgctctgg ttcaccggac tctcaggcgc cggcaagacc accatttccg
     4801 agatcgtcgc ccgggaactg accgcccgcg gctccaagct cgagattctc gacggtgaca
     4861 tcgtccgcga gaacctctcc aagggcctcg gattctcgaa ggaagaccgc gacaccaaca
     4921 tccgccggat cgccttcgtc gccgacctgc tttcccgcaa cggcgtgccg gtgatcaccg
     4981 ccgcgatttc tccctaccgg gcgatccgcg acgaagcccg cgacctgatg ggtgaccgct
     5041 tcatcgaggt ctacatcgag gcttcggtcg aagtctgcga ggcccgtgac gtcaaggggc
     5101 tctacgccaa ggcccgttcg ggcgagatcc cggagttcac cggtgtatcc gacccctacg
     5161 aggctccgga gaacccggag atcgccctca agacggcaga agaggagccg gaggagtcgg
     5221 ccgcccggct gatcacctac ctggaggagc gccagctcat tccggccgcc gtcgcctcct
     5281 agggacggcc gctgcaagga cgcaggaacc accccgaccg gggtttctgc tcctctcgcg
     5341 aattcggggc gggccttcgg gtccgccctt tcgtttgccc actaaacaac tgtttgattg
     5401 tgaagtggta caatgcccgc atgtccgttt ccctgaacat ccagaggtca ggagccccgg
     5461 aggggcatgc gccccaacgg gtacccctgg ttgacgagga cggactggtc acgatcgacc
     5521 cctggtgggg aacgatccag ccgcagcggc ttcaccccga tatcccgact tggggggagc
     5581 ttgaagtcat cgaacacatc gaggcgggcg gtcgtcttat cgacacccgt ttgcccgaat
     5641 acgtcgaggc gagtggaacg atccctggtt cactcgtcct gccgtgggaa acgatcaccg
     5701 accaccagga agtgatcgat tccgaatccg tgacggtgct cttctgcaac gggccgcaat
     5761 gcgcggcaac cccccaggcg gtgggaaggc tgctggacgc cggggtcgag cccgaggctc
     5821 tcgcctatta ccgaggcggg atccaggact ggatcgggct cggcctcccc accgttcctg
     5881 ccccaccaga ctgaccaaag gcttccgggt gggacgaccc ggacgaaggt cgcgatggag
     5941 cccccgcccc cggcggctcc attcgcggct gcctggatta ggtacctggt ccttcggtac
     6001 ccggtccttc gagggtcagg gcttcggggg aactgccggc tcgcccagat cgtaggtctg
     6061 gtacgcgccg aagcggctga aggtcttgtg ggtcgggccg tggtagtccg acgaggcggt
     6121 ggccgtgact ccgagctcgt ttgccaggtc aagcagatgc ttcgtctgcg ccctggtgtg
     6181 tgaggggtag aagacctcga tgccgccaat gtccagggcc cggatcagct cctcgacctg
     6241 ctccggcacc ttgatgtccc agtaagggtg ggcgatcacc ggaacgccgc cggcctcgcg
     6301 gatcagggcg caggcctcgt tcgcatccgg ccacttgcgc gagacgaagg ccttggcgcc
     6361 cggcaccagc cactcctcga agaaagggcc gaggttgccg gtcgcaccgg ccgccttggc
     6421 gatgtgggga cggccgatgg cgtcggccgc cccggcctcg cgaaccgcgt cctcgaaggt
     6481 caggtcgaat ccgtaggaac gcaggttctc gacgatctcc ttcgcccggt ttcgcctctc
     6541 ctgctgggcc cgctcgcagg ccggagtgat cttctcgaca tcgacccagt agccgcagat
     6601 gtgaaggtcc tcggcgtact cgtggaccga ggacatctcg accgcgggca cgatctcaac
     6661 gcccagccgc tccccggcct cgattgcctc aggcaccccg gccaccccgt cgtggtcggt
     6721 cagggcgagg acttccaccc cggccttggc cgcttcttct acaactcccg cgggctcaag
     6781 ctgaccatca gagataacgg aatgcgactg aagttcgatc atcgccgcga atcctaagcg
     6841 cccgccccga cccgcgcccg gaccaacacc tcgccggagt ttcgcccatc aagatcgagg
     6901 agtcaggaag cgttcactat gaacgcttcc tgactcctcg atccagccgg agggcgtgag
     6961 gtgggccgtc tgccgtacag ccgaagctgt agcggtcgga gaacgctttt tgggctcggc
     7021 aaatagactg ccccggctat gttttcgaag atcctgatcg ccaaccgtgg cgaaatcgct
     7081 atccgtgtcg cccgggcttg caaggagatg ggcattacct ccgtcgccgt ttattcggag
     7141 gccgatcggg atgccccgca tgtcaaggcc tgtgacgagg ctttcctgat cggacctgcg
     7201 gttccggccg aaagctacct ctcgatcgag aagatcatcc aggcctgccg ggactccggt
     7261 gccgaggccg tccaccccgg atacggcttc ctcgcggaga acgccgagtt cgcccgggca
     7321 ctcgacgagg ccgggatcaa cttcatcggc ccgcccgcct cggcgatcga cgcgatgggt
     7381 tcgaagacca tggcccgtga ggtgatggag aaggccggcg tgccgatcgt tcccggcgcc
     7441 accgagcccg ccaaggacct cgacgaggcg aaggcccagg ccgaagccgc cggttacccg
     7501 gtcgcctgca aggcggtcgg tggcggtggt ggcaagggct tccgcgtcgc catgagcccg
     7561 gacgagctgc aggaggcttt cgagggatcg gcccgcgagg gcgagaagtt cttctccgac
     7621 gaccgggtct acgtcgagcg ctacctcgaa gatccccgtc acgtcgaggt gcaggtgctg
     7681 gccgacaagc acggcaacgt gatccacctc ggcgagcgcg actgctcgat ccagcgccgt
     7741 caccagaagg tgatcgagga agctcccggc ccgcatgtgg acgaggagat gcgcgagcgg
     7801 atcggcaaga tcgcgaccga cgccgcgagg gcagtcggct acttctcggc cggcaccatc
     7861 gagggcatgc agcacggcga cgagtatttc ttcctcgaga tgaacacccg ggtccaggtc
     7921 gagcattgcg tgaccgagat gatcaccggc gtcgacatcg tccgcgagca gatcaagatc
     7981 gcctacggcg aggaactctc gatcagccag gaggacgtcg agatcgacgg ctgggcgatc
     8041 gaatgccgga tcaacgccga gaaggcggac aagaactttg ccccgtctcc cggtccgatc
     8101 gacgtctata acgagccgtc cggcccgggc gtgcgggtcg actccggagt caaggccggc
     8161 tccgaggtca ccccgatgta cgacccgatg gtcgccaagc tgatcgtctg ggacaaggac
     8221 cgtgaatccg cgacgaagcg gatgctgcgg gctctcgacg agtacgagat cgccccgctc
     8281 tcgaccctga tccccttcca caagaccatc ctcaggaccg agcaatgggc ccgcggcgag
     8341 acctgccgcg aactgatgga agacaagaag tggctgaaga ccaccgcgcc cgaggacaac
     8401 tcgattccgg agccggccga aggcgaagcc gagaaggtcg cccgggacta cctggtcgag
     8461 gtttccggca agcgcttcga cgtcaaggtg atcggcgaag cgaccggctt cgccgcaggc
     8521 gcggccccgg ccggcggcgg ccccaagccg aagcgggaga agaaggccgg cggcggtggc
     8581 ggtggaggcg ggccggtgct cgactcgccg ctgcagggtt cgatcttcaa gctcgaagtc
     8641 gaagagggcg ccgaggtggc cgagggcgac ctgatctgcg tgatcgaagc aatgaagatg
     8701 gagaacgaga tcaccgccca caagggcggc aaggtcacca aggtcagcat ttccgttggc
     8761 gatgcagtcg cttccgggga tcccctcgta act
//
LOCUS       DAOTIP010000007         8282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_10381_length_8282_cov_3.169685, whole genome shotgun sequence.
ACCESSION   DAOTIP010000007 DAOTIP010000000
VERSION     DAOTIP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_8282_cov_3.169685"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..366
                     /gene="rpsR"
                     /locus_tag="P5138_00305"
     CDS             109..366
                     /gene="rpsR"
                     /locus_tag="P5138_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRV59047.1"
                     /translation="MAKTREASSRRGRRFNERVRPRKYTKISAENIDYKDLTLLRRFI
                     SDKGKTRSRRVTGLSRKHQAQLSVAVKRAREVALLPYVGEK"
     gene            366..818
                     /gene="rplI"
                     /locus_tag="P5138_00310"
     CDS             366..818
                     /gene="rplI"
                     /locus_tag="P5138_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRV59048.1"
                     /translation="MAQAILLADVEGLGENGTAVEVSPGYLRNFLQPRGLAQPATPGA
                     LEEAIRRKEAAEKAEREAIARAKENASLLSKTVLTINHRAGDDGKLYGSVTTKEIADA
                     IRDARGIKVDRKKIRLDVPIHQVGTYMIDVEVAGGAIASVKTIVAEEK"
     gene            839..2245
                     /gene="dnaB"
                     /locus_tag="P5138_00315"
     CDS             839..2245
                     /gene="dnaB"
                     /locus_tag="P5138_00315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRV59049.1"
                     /translation="MDAEEAVLGSMLVYEPAVNWAIDEVKLNPADFYLDRHKAIFEVM
                     LDLYTTDKACDSLTVTEALVSAGRLEEAGGNNYVAELAEKVAAPGNARHHAEIVKETS
                     HLRRLLLAAQDIEAWVGERKASARELSDQAETRLFEVAHKEQEGDFRKLDDVLFDEAD
                     RLHKRITGELKASGVPTGFDDLDEKTGGLQPSNLIILAARPSVGKSAFAANIAENIAV
                     EQGRAVAFFSLEMSESELAQRFIASQSGVKGDLLRKGRLSTEKGKDRDWKKVTRALNQ
                     LNDAPLYIDVTSDLGLLDLRAKARRLHEQEIRRNEGTEREGEGLSLIVVDYLQLMRMD
                     ERIANRAEQVGQVSRGLKLLSSELGVPLIALSQLNRANENRPDKRPMLSDLRESGNIE
                     QDADLVLFLYRDDVYKKDPVERDGTAEVLISKNRNGPIGMSPLVFMHAITRFVKASHE
                     HTAGEGSPVAVRAESETV"
     gene            2245..2979
                     /locus_tag="P5138_00320"
     CDS             2245..2979
                     /locus_tag="P5138_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV59050.1"
                     /translation="MAVEEYGTPANGLPPCPFGRCDGSGWILNEDNTATKCDCLEPRL
                     ARARASGINSVLPKRYRGVSFDRPPVTDISPGAVSIVREWVDQLDQNLEQGNGLWLMG
                     DTGTGKTTLAMLVSKEALKRAHTVAIYSMPKLLSRIRATYGAEAGEESYDQFFERLCE
                     VDLLHIDDLGAEKQTEWVLEQLYALVNERYERQKSIVVTTNLTQDELEEQIGPRTVSR
                     LIQMCGENQIPLFGDDARQKPQLRDG"
     gene            3036..4367
                     /locus_tag="P5138_00325"
     CDS             3036..4367
                     /locus_tag="P5138_00325"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRV59051.1"
                     /translation="MPGLVLVGTQWGDEGKGKITDLLAKDASAIVRFQGGNNAGHTIV
                     HDGEEYKFHLIPSGILHEDKYCVIGNGVVVDPRVLLEEIEGLHKAGVSASNLRVSSNA
                     HLIMPYHVLVDTAEEIKLGKLSIGTTRRGIGPAYADKVSRIGIRVQDLLDEKILRKKI
                     MAALEHKKKSLQELAVRRRKLHKEAEQDEEVQIDDRLDLHTITEQYLSYGHRLEPYIA
                     DTARLCWAELDAGNKVIFEGAQAALLDLDHGTYPFVTSSNPIAGAACVGAGVGPTDIQ
                     EVWGIVKAYSTRVGSGPFPTELFDEVGEYMSDRGHEFGTTTGRRRRCGWLDLVALKYA
                     VRLNRLSHLAMTKLDVLTGLDTIKVAVRYRSKEGALLEDFPYHQTILHNATPEYVDLP
                     GFSEDITGCRTEEDLPPAAREYISFVSDFVGVPISLVGVGPGREEVVWIGD"
     gene            complement(4663..5406)
                     /locus_tag="P5138_00330"
     CDS             complement(4663..5406)
                     /locus_tag="P5138_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59052.1"
                     /translation="MIRELAEAASRLRPRRETHSHWRAIRSDRATAALAVGTIAIAGL
                     VVAAQYSRLLSRRTHSTESDGLIDSAPAAAVDTVGVAVEGYSATPNRELVLFNLLSGF
                     LGSFALVRLTTWAIREDWGPFRNVRVGGRHIHHFVPGILIGFGSGVSALLVNGENADR
                     RLARTLGIGMGLTFDEAALLLDLQDVYWTRQGLLSVQITLATGATLGAAVLTMRILGR
                     GEARQEEAGEIPAEEGPVNAVVPWPHPVT"
     gene            complement(5414..>6611)
                     /locus_tag="P5138_00335"
     CDS             complement(5414..>6611)
                     /locus_tag="P5138_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRV59053.1"
                     /translation="AESGREVIGLEANASRVEALNRSESYIEDIPSETLARLDDRMKA
                     TGDYAELADCEAVIICVPTPLTGSREPDLSYLESAADNLAEVMTPGQTIVLESTTYPG
                     TTRGLLATTLEAGGRKTGTDFHLAFSPERIDPGRTDFTVRTTPKLVGGMTPACTEKAV
                     ALYEAICDRVVPLSGPEAAELSKMFEHIFRSVNIALVNELAQLCDRLGVDVWEVIDAA
                     ATKPFGFMRFDPGPGMGGHCLPVDPFYLAFKAREHDFYPEFIELAGKVNQSQPLFCVN
                     RIERVLNDNAKPVNGTTILILGVSYKGGVGDTRESPAIKIIRLLRELGAEVSYHDPHV
                     PELKEAGLSNSPDIGNALDEVDLSVIVTAHPEFDWEEIISRSPLVFDLRGVTRGFEDS
                     GNVTRL"
     assembly_gap    6612..6621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6998..>8282
                     /locus_tag="P5138_00340"
     CDS             6998..>8282
                     /locus_tag="P5138_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HRV59054.1"
                     /translation="MNLEQWVALAAGCFLLVLTVLVARRSQARGDGVLTALSLFGLVW
                     GLALVLFAVPLVEYTTTPAKVWLMVYGSIATFVGGALLAERRYRNLPHADQSERQRPD
                     PRRLRIAWILFAILGLIGFVAYVHAVDVTIGWQAIFNDPETARAIQTTSPLFQDTYGP
                     WKLLTYCNQIAFILWTLGLREKAFSGRWRPVMFLGILSLAPFIFTGDRTLMIAALIWA
                     GAFQLLYLPPLKPARLLGGFAVAAVLLTILFSVLGERVGKTVDGQPQIAETLTTRSFD
                     PLVLPFVYATGNIPAFGGLVDDPIRPDTYGQLTFSSATKLLHGFGLWGQPAEMVGAFY
                     AIPFQTFNNYSWLGIFYSDFGVLGVLLLPFIFAFLTTAVVLRAIRRRTLLSVWVASLL
                     LYCVSFTFDGYKFFDTLVSEYLLAGLVIVPLIRADL"
BASE COUNT         1544 a         2676 c         2543 g         1509 t
ORIGIN      
        1 cgaatcggtt gatacagaag tcgcataggg ccgggaaccg ctacgattct tggtcctgaa
       61 cgcccggact ttcttccggg ccgcaactat gtaaggcacc ggaaagcatt ggctaagaca
      121 cgagaagcat catcgcgacg cgggcgccgc ttcaacgagc gcgtacgccc ccgcaagtac
      181 accaagattt cagccgagaa catcgactac aaggatctga ccctgctgcg tcggttcatc
      241 tcggacaagg gcaagacccg ctcccgcagg gtcaccggcc tttcccgcaa gcatcaggcc
      301 cagctgtcgg tcgcggtcaa gcgcgcccgc gaagtggccc tgctcccgta cgtgggtgag
      361 aagtaatggc ccaggccatt ctgctcgctg acgtcgaggg cctgggggag aacggcaccg
      421 ccgtcgaggt ttcgcccggc tacctgagga acttcctcca gccccgtggc ctggcccagc
      481 cggccacccc gggtgctctc gaggaggcga tccgccgcaa ggaagccgcc gagaaggctg
      541 agcgcgaggc gatcgcccgg gccaaggaga acgcatccct gctctccaag accgtgctca
      601 cgatcaacca tcgggctggc gacgacggca agctctacgg ctcggtcacg accaaggaaa
      661 tcgccgacgc gatccgcgat gcccgcggca tcaaggtcga ccgcaagaag attcgcctcg
      721 acgtgccgat ccatcaggtc ggcacctaca tgattgacgt tgaagtggcc ggcggcgcaa
      781 tcgcctcggt caagacgatc gtcgcggaag agaaatagcc caggtaccac ccaacaacat
      841 cgacgccgag gaggcagtcc tcggctcgat gctggtctat gagcccgccg tcaactgggc
      901 catcgatgaa gtcaagctga acccggccga tttttacctc gaccgccaca aggcgatctt
      961 cgaggtgatg cttgacctgt acaccacgga caaggcctgt gattcgctga ccgtcaccga
     1021 ggcgctggtc agcgcgggaa ggctcgagga agcgggcggc aacaactacg tcgcggaact
     1081 ggccgagaag gttgcggccc cgggcaacgc ccgtcatcac gccgagatcg tcaaggagac
     1141 atcgcatctg cgtcggctgc ttctggccgc ccaggacatc gaagcctggg tcggtgaacg
     1201 caaagcaagt gcccgtgagc tttccgatca ggcagagacc cggcttttcg aagtcgctca
     1261 caaggagcag gagggtgact tccgcaagct cgatgacgtc ctcttcgacg aagccgaccg
     1321 tctccacaag aggatcaccg gagaactgaa ggcctcggga gtgccgaccg gcttcgacga
     1381 cctcgacgag aagaccggcg gactgcagcc gtcgaacctg atcatcctgg ccgcccggcc
     1441 ttcggtcggg aagtcggcct tcgccgcgaa catcgccgag aacatcgcgg tcgagcaggg
     1501 cagggcggtc gccttcttct ctctggaaat gtccgagtcc gaactcgccc agcgtttcat
     1561 cgcctcgcag tccggggtca agggcgacct tctgcgcaag ggcaggctga gcacggagaa
     1621 gggcaaggac cgggactgga agaaggtcac cagggccctg aaccagttga acgacgcccc
     1681 gctttacatc gacgtcacct cggacctcgg cctgctcgac ctgcgggcga aggcgcgacg
     1741 gctccacgaa caggagatca ggcggaacga aggcaccgaa agggaaggcg agggcctttc
     1801 cctgatcgtc gtcgactacc tccagctgat gcggatggac gagcggatcg ccaaccgtgc
     1861 cgagcaggtc ggtcaggtca gtcgtggcct gaagctcctt tccagcgagc tcggggtgcc
     1921 gctgatcgcc ctttcccagc tcaatcgtgc gaacgagaac cggccggaca agaggccgat
     1981 gctctccgac ctgcgtgagt cgggcaacat cgagcaggac gccgacctcg tgctcttcct
     2041 ctaccgggat gacgtctaca agaaggatcc tgtcgagcgt gacggcaccg ccgaggtcct
     2101 gatctcgaag aacaggaacg gcccgatcgg catgagcccg ctggtcttca tgcacgcgat
     2161 aaccaggttc gtcaaggcca gccatgaaca cacggccggc gagggttccc cggtcgcagt
     2221 gcgggccgag agtgaaaccg tctgatggcg gtcgaggaat atggaacccc ggcaaacggc
     2281 ctgccgccct gtccgttcgg acgttgtgac ggcagcggct ggatcctgaa cgaggacaac
     2341 acggcaacca agtgcgattg cctcgagccc cgtctggccc gggcccgggc ttccgggatc
     2401 aattccgtac ttccgaagcg ttaccggggg gtctccttcg accggccacc ggtgaccgac
     2461 atatctcccg gggccgtctc catcgtccgc gaatgggtcg atcagctcga ccagaacctc
     2521 gaacagggca acgggctgtg gctgatgggc gacaccggca ccggcaagac caccctcgcg
     2581 atgctggtct cgaaagaagc cctcaagcgc gcccacacgg tggcgatcta ttcgatgccg
     2641 aaactgcttt cccggatccg cgccacctac ggagccgagg ccggcgagga atcctatgac
     2701 cagttcttcg agcggctctg cgaggtcgac ctcctccaca tcgacgacct cggggccgag
     2761 aaacagaccg aatgggtcct cgagcagctg tacgcgctgg tcaacgaacg ctacgaacgg
     2821 cagaagtcga tcgtcgtcac gaccaacctg acccaggacg aactggagga gcagatcggt
     2881 ccacgcaccg tgtcgaggct gatccagatg tgcggcgaaa accagatccc gctcttcggc
     2941 gacgacgccc ggcagaagcc gcagctgagg gacggctgat acctgtaccg gcggatagcc
     3001 gcggccgaac ggcttccatc tagactcaca cactcatgcc aggactcgtt ttagtaggaa
     3061 cccagtgggg tgacgaaggc aagggcaaga tcacggatct gctcgccaag gacgcctccg
     3121 cgattgtgcg tttccagggc ggcaacaacg ccggccacac gatcgtgcac gacggcgagg
     3181 aatacaagtt tcacctgatc ccgtcgggca tcctgcatga ggacaagtac tgcgtgatcg
     3241 ggaacggagt ggtggtcgat ccacgggtcc tgctggagga gatcgagggt ctccacaagg
     3301 ctggggtctc cgcttcgaac ctgcgggtct cctcgaacgc ccatctgatc atgccctacc
     3361 acgtgctggt cgacacggcg gaggagatca agctcgggaa actctcgatc ggaaccacgc
     3421 gccgcggaat cggcccggcc tacgccgaca aggtttcccg gatcgggatc cgggtccagg
     3481 acctgctcga cgagaagatc ctgcgcaaga agatcatggc cgcgctggag cacaagaaga
     3541 agtcgctcca ggagctggct gtccgccgtc gaaagctgca caaggaagcc gagcaggacg
     3601 aggaagttca gatcgacgac cggctggacc ttcacacgat caccgagcag tacctcagct
     3661 acggccatcg gctggagccc tacatcgccg atacggcgag gctctgctgg gcggaactcg
     3721 acgccggcaa caaggtgatc ttcgagggtg cccaggccgc cctgctcgac ctcgaccatg
     3781 gcacctatcc cttcgtcacc tcctccaatc cgatcgcggg tgccgcctgt gtcggggccg
     3841 gggtcggccc gaccgacatc caggaggtct ggggcatcgt caaggcttac tcgacccggg
     3901 tcgggtcggg accgttcccg accgagctgt tcgacgaggt cggcgaatac atgtcggacc
     3961 gtgggcacga gttcggcacc acgacgggcc gccggcgccg ctgcggctgg ctcgatctcg
     4021 ttgccctcaa gtacgcggtc cggctgaacc gcctctcgca tctggcgatg accaaacttg
     4081 acgtcctgac cggactcgac acgatcaagg ttgcggtccg gtaccggtcg aaggaagggg
     4141 ccctgctgga ggattttccc taccaccaga cgatcctcca caacgcgacc cccgagtacg
     4201 tcgaccttcc cgggttctcc gaggacatca caggatgccg gaccgaggaa gacctcccgc
     4261 cggcagcccg ggagtacatc tctttcgtca gcgatttcgt cggcgttccg atcagcctgg
     4321 tcggggtcgg cccgggccgc gaagaagtcg tctggattgg cgactgagaa tctttaagaa
     4381 atcttttttc tttccggaca cataaacagg tgtgtttggt gtactatgtc gtatcagcag
     4441 tttgatcact ggacgtgata gctgcaagac atcgttgact ggggtccctc cctaccctca
     4501 gcagcgctta gtcggtgaag tcgggttggc ccactctccc ttgggtcgac ccgaccaccg
     4561 acatttttta tgcctgtttg ccgcatggga atggggtttt ggctccagga ctcccggagt
     4621 gcgaacaaat tccgtaaacg ttgcgtcttg gtacagggat gtctacgtaa ccggatgagg
     4681 ccacggtacg accgcgttca ccggaccttc ttcggccggg atctctcccg cctcctcctg
     4741 cctcgcttcg ccccggccga ggattcgcat ggtcagcacg gctgcaccga gcgttgcgcc
     4801 ggtcgccagg gtgatctgga ccgagagcaa cccctgcctc gtccagtaga cgtcctggag
     4861 atcgagcagc agcgccgcct cgtcgaaggt gagtcccatc ccgatcccga gagtccgggc
     4921 aagccgccgg tcggcattct cgccgttcac cagcagggcg ctcacccccg agccgaaacc
     4981 gatcaggatc cccggaacga agtggtggat gtgccggccc ccgaccctga cgttccgaaa
     5041 cggaccccag tcctcccgga tcgcccaggt agtcaggcgg accagcgcga agctgccgag
     5101 gaatccggaa agcaggttga aaagcaccag ctcccggttg ggagttgccg aatagccctc
     5161 gaccgcaaca ccgaccgtgt ccacggccgc ggccggggcg gagtcgatca ggccatccga
     5221 ttcggtcgag tgggtacgcc ggctgagcag gcggctgtac tgcgcggcga ccacgaggcc
     5281 ggcgatcgcg atcgtgccga ccgccagggc ggccgtcgcc cggtcgctgc ggatcgcccg
     5341 ccagtgggaa tgggtttccc ggcggggtct gagccttgaa gctgcttctg ccagttcccg
     5401 gatcacgaaa ggctcagagc cgcgtgacgt tcccggaatc ttcgaagccc ctcgtgactc
     5461 cgcgcaggtc gaagaccagc gggctgcggg agatgatctc ttcccagtcg aactcggggt
     5521 gggcggtgac gatcacgctc aggtccacct cgtccagggc gttgccgatg tcgggcgagt
     5581 tgctcagccc ggcctccttc agttccggca cgtgagggtc gtggtagctg acctcggctc
     5641 ccagctcccg gagcagccgg atgatcttga tcgccgggga ctcccttgtg tcacccacgc
     5701 cgcccttgta gctgaccccg aggatcagga tcgtcgtccc gttgaccggt ttcgcgttgt
     5761 cattcaggac ccgttcgatc cggttgacgc aaaagagcgg ctgcgactgg ttgaccttgc
     5821 ccgcgagctc gatgaactcc gggtagaagt catgctcgcg ggccttgaag gccaggtaga
     5881 agggatcaac cggcaggcag tgcccgccca tccccggtcc ggggtcgaag cgcatgaacc
     5941 cgaacggttt ggtcgccgcc gcgtcgatca cttcccagac atcgaccccg agcctgtcgc
     6001 agagctgggc aagctcgttg accagcgcga tgttgaccga tcggaaaatg tgctcgaaca
     6061 tcttggagag ctctgccgct tccggacccg aaagcgggac cacccggtcg cagatcgctt
     6121 catagagcgc cacggccttc tccgtgcagg ccggagtcat gcccccgacc agcttggggg
     6181 tggtgcgaac cgtgaagtcg gtgcggccgg ggtcgatccg ctccggcgag aaggccaggt
     6241 ggaagtcggt gccggtcttc cggcctcctg cttccagcgt ggtcgccagc aggccgcggg
     6301 tggttcccgg gtaagtggtc gactcgagca cgatggtctg gcccggggtc atcacttccg
     6361 ccaggttgtc agcagccgac tcaaggtagc tcaggtctgg ttccctcgat ccggtcagcg
     6421 gggtgggaac gcagatgatc accgcctcgc aatcagccag ttcggcgtaa tcgccggtgg
     6481 ccttcatccg atcatcgagc ctggcaagag tctccgaagg gatgtcctcg atgtagctct
     6541 cggaacggtt gagcgcttcc accctggagg cgtttgcctc gaggccgatc acctcccgac
     6601 cggattcggc annnnnnnnn ngcgagtggc agacccacgt agcccatgcc gacgatcccg
     6661 attttccgga agtccttctc cggagatgaa gcattcaagg cgctgttccc tttcattcgt
     6721 gtgaagttct ttgtcccgat gcgcgatagc ggccgctgtt tcgactgcac atcgtggaat
     6781 cacttgttca cttccctgcc tgtgcgtcgc atccaaggcc agacggcgaa cagaacaaga
     6841 caaactatat ggacaacaag tccggccgac gctatccccc cgcggggatt tcaccttgct
     6901 agcctgccgt ggtcatgttc ccgaacgatt tcacggggtt cactcccggt cgggaccgac
     6961 agttctgggt ccctcccaat ccctgttccc acgcacaatg aaccttgagc agtgggtagc
     7021 cctcgcggca ggctgtttcc tcctggtcct caccgtcctg gttgccaggc ggtcccaggc
     7081 ccgaggagac ggcgtcctga ccgctttgag tctcttcgga cttgtctggg gtcttgccct
     7141 ggtcctcttc gcggtaccgc tggtcgagta cacgaccacc ccggccaagg tctggctcat
     7201 ggtctatggg tcgatagcga cattcgttgg cggtgcgctg ctggccgaac gccggtaccg
     7261 caatcttccc catgccgatc agagcgaacg acagcgcccg gacccgcggc ggcttcgtat
     7321 cgcctggatc cttttcgcga tcctgggtct gatcggtttc gtcgcgtatg tccatgcggt
     7381 ggacgtgacg atcgggtggc aggcgatctt caacgacccg gaaacggccc gggccatcca
     7441 gacgaccagt ccccttttcc aggacaccta tgggccctgg aagctgctca cctactgcaa
     7501 ccagatcgca ttcatcctct ggaccctggg cttgcgcgag aaggccttct ccggacgctg
     7561 gcgcccggtc atgttcctgg ggatcctctc cctcgcaccg ttcatcttca cgggcgatcg
     7621 cactctcatg atcgctgccc tgatctgggc tggcgcattc cagctgctgt atctgccgcc
     7681 cctaaagccg gccaggctgc ttggcggttt cgccgtcgcc gcagtcctgc tcacaattct
     7741 cttcagcgtc cttggcgaac gagtgggaaa gacggtagac ggacaaccgc agatcgccga
     7801 gacactcacg accaggtcat tcgaccccct ggtcctgcct ttcgtgtacg cgaccggcaa
     7861 cattccggcg ttcggcggac tggtcgatga tccgattcgc ccggacacct acgggcagct
     7921 gaccttcagc tcggcgacca agctgcttca cggattcgga ctttgggggc aaccggccga
     7981 aatggtcgga gctttttacg caattccgtt ccagaccttc aacaactact cgtggttggg
     8041 gatcttctat tccgacttcg gagtattggg cgtgctgctg cttccgttca ttttcgcttt
     8101 cctcacaacg gccgtcgtgc tcagggcgat tcgacgccgg accctcttaa gcgtctgggt
     8161 ggcttctctc ctcttgtact gcgtctcctt cacctttgat ggatacaagt tcttcgacac
     8221 gctcgtttcc gagtacctcc tggccggctt ggtgatagtg cctctgatca gggccgacct
     8281 gg
//
LOCUS       DAOTIP010000008         8037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_10810_length_8037_cov_3.517164, whole genome shotgun sequence.
ACCESSION   DAOTIP010000008 DAOTIP010000000
VERSION     DAOTIP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10810_length_8037_cov_3.517164"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..702)
                     /locus_tag="P5138_00345"
     CDS             complement(79..702)
                     /locus_tag="P5138_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRV59055.1"
                     /translation="MRSIDQAVADHGWWLASRASGLVALVLVTISVAIGLMMASKIAR
                     RPGMPKLLAAIHEQTALAGLLAIAVHGITLIGDPWLHPGVGGVLVPFSMDYRPLWTGL
                     GTIAGIMAMVLGLSFYIRKNIGAKLWRKAHRATIIVYFLAIGHTLGAGTDASTTWMRW
                     WLILTTPPIVILFIYRVVSPSLRNRGRPSRSVAAASALAEARAGERG"
     gene            complement(699..1703)
                     /locus_tag="P5138_00350"
     CDS             complement(699..1703)
                     /locus_tag="P5138_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRV59056.1"
                     /translation="MRDGTVEPYDEQFDAMGCTVRVMIDPSSGDAGRDPREAGRQARE
                     FILDFDRRLSRFRADSELTALNRDLREEVPASDLLRAAVRAGLRAARSTNGLVDPTLV
                     DQIEQAGYRESRAGLAGVPIPELLSSAPTRESASPSPGRSWRMFEVDDERGIIRRTPG
                     LRFDTGGTGKGLAADMVAGSLADFPRFLVNCGGDIRIGGNEASGSPYDVLVEHPASGN
                     MPHRFKLGRGGIATSGINVRAWLREGGSPGHHLLDPSTGEPCWSGLLGVTAIGPTAVE
                     AETLAKAALLSGPTGARKILSHHGGLMVHEDSQVEMVGRATIRLRLPEVRLRTSEVAV
                     "
     gene            complement(1700..2059)
                     /locus_tag="P5138_00355"
     CDS             complement(1700..2059)
                     /locus_tag="P5138_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59057.1"
                     /translation="MTEGRTVTRRRKPSAATVFLASAALFVAALGYLGYQLAAGRDPA
                     LGPNATVAEKQTRPLVIHRRIIRRRIVTTVVPSPVTVSSSTVSSGSAPTSSYSAPAPV
                     SAPAPVAAPAPVVSASS"
     gene            complement(2052..2486)
                     /locus_tag="P5138_00360"
     CDS             complement(2052..2486)
                     /locus_tag="P5138_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59058.1"
                     /translation="MENVTVKGAVSVTLLALGGLAGAAIASNGGSSSSDPEPAATLKP
                     RVRTEVIRRTIHRTKMAKPASVEGGTVQSAPAAAAPAPSTSSYTSAPVYTPSTSSSSS
                     QPAVTSTSGSASSGGSYSPEESQESETGYESGDRQESEGQDD"
     gene            2632..3360
                     /locus_tag="P5138_00365"
     CDS             2632..3360
                     /locus_tag="P5138_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV59059.1"
                     /translation="MDSRGRVKPRILFVEDEDSISEPFTRSLEKAGFEPVNARTSASA
                     LELFERLTPDFVLLDLGLPDGDGRDVCVEIRRRSDVPILMLTASGTETDRVVGLELGA
                     DDYVVKPFSAREVISRIRAILRRSSRTGPDPAAEPIRVNDLVVDIPGRTATVAGSELK
                     LSRKEFDLLAELARNSGTVVTREDLMADVWDVNWFGSTKTLDVHIRALRKKLGDSATE
                     SRYIQTVRGVGFRLAPQERNDSKP"
     gene            3371..4744
                     /locus_tag="P5138_00370"
     CDS             3371..4744
                     /locus_tag="P5138_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRV59060.1"
                     /translation="MAAVGYVLVLALIALGLPLALSLKDRVNDEVRSQAHSQAAIVAT
                     GAGSLVQPPNETALNRLVRSASETVQGRVLIVDKAGLVLSDSASAETAGSDYSSRPEI
                     AGALQGSSEQFERTSDTLGERILATADPIQAGNRVTGAVRITQSVDEVHAAINKSLLG
                     LGLLALLVLAFALLVAAVIANQISRPIRRLEVSARHFASGEVEEKAEIFGSREQRSLA
                     RSFNEMTSRVKRLLKSQSDFVASASHQLRTPLTGLRLRVEALRDDAENEESRAELDSG
                     LAEIDRLSRMVDELLLLSRAGEVDVPGEDLDLGEIAREARGRWNRASGGRTLVLTGAD
                     RKDIDAVFSARADLDRILDVLIENAIHYSPEESVVEIQVVPAGIRVLDEGSGIDPEER
                     EAVFQRFERGRSGREGRKGTGLGLAIARELAIPWKAKISVEPRQPAGTIASVSFAKRT
                     DSESSGS"
     gene            4788..5180
                     /locus_tag="P5138_00375"
     CDS             4788..5180
                     /locus_tag="P5138_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59061.1"
                     /translation="MTDRKEAIPGEWGRWRWIALLACGIVLAVAIALVAGSLAHQPVG
                     LSGEPVSAGSALVPANVTRIESGGSGQKTGALKRSAGETEGKSTDEPSGQSGPVTSGG
                     SSQPETQSSIGEGTGGSGDDGGGEAEDD"
     gene            5225..5761
                     /locus_tag="P5138_00380"
     CDS             5225..5761
                     /locus_tag="P5138_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59062.1"
                     /translation="MRIRTAGAVLLVAGATVAGCGGGGGGGEALESDYADGTEEAAVV
                     KTVSDFNIAFANKDGEKACSYLTSGGQKNMENAVGDLPPDKSCEDLVSSRLEHGRIEG
                     GGINTGDMWNSLWLDVGDDGNHFLNSHDLDEAIVVKVNGQVARLTAPGRNGLTYLLTE
                     HDGEWLIEAVGDENFNGE"
     gene            complement(5790..7886)
                     /locus_tag="P5138_00385"
     CDS             complement(5790..7886)
                     /locus_tag="P5138_00385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRV59063.1"
                     /translation="MALGGAEVAARESGSPTAKEAGSNFRPDIQGIRAIAVTLVLACH
                     ASIPWAKGGFIGVDVFYVLSGFLITGLVVREVEKTGKVSIRNFYARRAKRLLPMAALV
                     LVFICIVTLFVFSLARQVEIGGDVFAAALYFVNWHFIIQGVDYFAAADGIHSPVQHYW
                     SLSVEEQFYLVWPLILMAAGALAAKFRRRTKGIVLIFIVPVAIASLIYSIHFTQVNPE
                     AAFFSTIARAWQLAFGAVLAMILPKKIGMPASLATVIGVIAVGVLGWATYTMSASTPF
                     PGSNGLYPVLATVALLVVGASGVGITTRILSTRPFQYLGNISYSWYLWHWPFVVFALA
                     IFDNLTWKWLVVATLLSWIPTQISHTLLEDPVRRARILKIKPNRALAIGGVCSIAAAA
                     FGLWLGSNRINVDIIDQDQVAGAAVVKNGHVPLVNSVDKIKPNPLHAREDRGQMYADG
                     CLLLGPRTESGDCTYGDPDGDVSVVLFGDSHSMMYMPALDAIGKEKGWKITGLSRANC
                     VIADVKLTKYCDEWRENSLERIKEEHPDLVVVATSTQAVYSLKKNGKQLSRIDSEPYL
                     VDGMARTFRKLKEDADKVILLRDQAQAPRQPWLPHECVAENLHNLKTCAFAPKRHPEL
                     GFDVDAAEKTRVQIIDPMPILCSDGRCPAVIGNALVYRDSYHVSATYMVTMAPWLQKE
                     LAKLDFPPGSSSGDSK"
BASE COUNT         1480 a         2457 c         2714 g         1386 t
ORIGIN      
        1 cgtaaccctt ctttcgcagg gtctgggcgc agcgcaaacc ggccagtcca ccaccgacaa
       61 tgagtacgcc gtcgccgttc atccccgttc acccgccctg gcctcagcga gcgccgatgc
      121 cgccgccacg ctgcgactgg gccgccctcg attgcgaagt gacggagaaa cgaccctgta
      181 gatgaacagg atgacaatcg gaggggtggt caggatcagc caccatctca tccaggtcgt
      241 agacgcatcg gttccggctc cgagggtgtg tccgatcgcg aggaagtaga cgatgatcgt
      301 cgcccggtgc gccttgcgcc atagcttcgc gccgatgttc ttccttatgt agaaactcag
      361 tccgaggacc atcgccatga ttcctgcgat cgtgccgaga ccggtccata gcggtctgta
      421 gtccatcgag aacggaacca gtacaccgcc gaccccggga tggagccacg gatccccgat
      481 cagggttatc ccgtggacgg cgatcgccag caaccccgcg agggcggtct gttcatgtat
      541 cgccgcgagc agtttcggca ttcccggtct tctcgcgatc ttgctggcca tcatcagacc
      601 gatcgcgacc gaaatggtca ccaggaccag ggcaaccagg ccggaggccc ggctggccag
      661 ccaccagcca tgatccgcta ccgcctggtc gatgcttctc ataccgcaac ctcgctcgtc
      721 cggagccgga cttccggcag acgcagcctg atcgtggccc gacccaccat ctcgacctgg
      781 ctgtcctcgt ggaccatcag gcctccgtgg tgggagagga tcttgcgggc acctgtcggc
      841 ccggagagaa gagctgcttt ggcgagggtt tccgcttcga cggccgtagg gccgattgcg
      901 gttactccga gcaggccgga ccagcacggc tcgccggtcg acggatccag aagatggtgg
      961 ccgggacttc cgccttcacg gagccaggcg cgcacgttga ttccggaagt cgcgattccg
     1021 ccccggccca gcttgaaacg gtggggcatg ttgccgcttg ccggatgctc gaccagaacg
     1081 tcgtacggcg agccggaggc ttcgttccca ccgatccgga tgtccccgcc gcagttgacc
     1141 aggaagcgcg ggaagtcggc gagcgacccg gccaccatgt cggcagccag tcccttgccc
     1201 gttccgccgg tgtcgaaccg gagaccgggg gtcctgcgga tgatgccccg ctcgtcgtcc
     1261 acttcgaaca tgcgccagga acgtccgggg gaagggctgg ccgattcccg ggtgggggcg
     1321 cttgacagga gttcagggat cggcactccc gccaatccgg ccctggattc gcggtagccg
     1381 gcctgttcga tctggtcgac gagggtgggg tcgacgagac cgttcgtgct gcgcgcggcc
     1441 cgcaaccctg cccgaaccgc tgctcggagc aggtctgacg cgggtacttc ttcccgcaga
     1501 tccctgttca gggcggtgag ctcgctgtcg gcacggaacc tggacagccg gcgatcgaag
     1561 tcaaggatga attccctggc ctgtcgtccg gcttcccggg ggtcccggcc tgcatccccg
     1621 gaggacggat cgatcatgac ccggaccgtg catcccattg cgtcgaactg ttcgtcgtac
     1681 ggttccaccg ttccatccct catgagctgg cggacacgac cggtgcgggg gcggccacgg
     1741 gcgcgggtgc cgacaccggt gccggcgcgg agtagctgct cgtcggcgcg gagccgctgg
     1801 aaacggtgct cgaggagacc gtcaccggtg agggaaccac cgtcgtcacg attcgccggc
     1861 ggatgattcg ccggtggatg accagcggcc tcgtctgctt ctccgccacg gtggcgttcg
     1921 gtcccagcgc cggatcccgt cccgctgcca actggtatcc gaggtagccg agggctgcga
     1981 cgaacagtgc ggctgaagca aggaacacgg tcgccgcaga aggcttccgg cgtcgggtga
     2041 cggtccttcc ctcagtcatc ctgaccctct gattcctgac ggtccccgga ctcgtatcca
     2101 gtctctgact cctggctctc ctcggggctg tagcttccgc cgctgctggc agaaccgctc
     2161 gttgaagtga ctgccggctg ggaactgctg ctgctggtcg aaggcgtgta gacgggcgcg
     2221 gatgtgtacg agctcgtcga cggggccggt gcagctgccg cgggggcgct ctggacggtg
     2281 ccgccctcca cgctcgccgg tttcgccatt ttcgtccggt ggatcgtgcg ccggatcact
     2341 tcggtccgaa cccgtggctt gagtgtcgcc gctggttcgg ggtccgaact tgaacttccg
     2401 ccattcgaag ctattgccgc cccggccagg ccaccgagcg ccagcagcgt caccgacacc
     2461 gcgcccttca ctgtcacatt ctccatggga ccaactcctc tgcttgattt cagggaaacc
     2521 cgattggttg gccagcagtc tgcggatgcc tgatcaaggc cccggacatc tggaggtaag
     2581 gacttggcaa agcttttctg tgggcatgga gggccgatgt aggatcgacc gatggattcg
     2641 cgcggacggg tcaagccacg aattctcttc gtcgaagatg aagactcgat ctcggagcca
     2701 ttcaccagat cccttgaaaa ggcgggtttc gagccggtta acgcccggac atcagcgtct
     2761 gccctcgagc tcttcgaaag gctgactccg gattttgttc tgcttgacct cggcctcccg
     2821 gacggcgatg gccgggacgt ctgcgtcgag ataagacggc gatcggatgt cccgatactc
     2881 atgctcacgg ccagcggcac ggaaaccgat cgggtggtgg ggttggaact gggagccgac
     2941 gactacgtag tgaaaccgtt cagcgcccgc gaagtgattt ccaggatccg ggcaatcctc
     3001 aggcgctctt caaggacagg ccccgatcct gccgccgaac ccatcagggt caacgatctc
     3061 gtcgttgaca tcccggggag gacggcaacc gtcgcggggt ccgagctgaa gttgtcgcga
     3121 aaggaattcg acctgctggc ggaactcgcc cgtaactccg gaacggttgt cacccgcgag
     3181 gacctgatgg cggacgtatg ggacgtcaac tggttcggct cgaccaagac gctcgacgtt
     3241 cacatccggg ctctcaggaa gaagctcggt gacagtgcca ccgagtcgcg ctacatccag
     3301 acagttcggg gagtcggctt ccgcctggcg cctcaggaga ggaacgacag taagccttag
     3361 agcgatactt gtggcggcgg tcggttatgt gctggtcctt gcgctgatcg cgctcgggtt
     3421 gcccctggcg ctgagtctca aagaccgcgt caacgacgaa gtacgcagcc aggctcacag
     3481 tcaggctgcg atcgtcgcaa ccggcgcagg cagccttgtc cagcccccga acgagacggc
     3541 gctgaaccgc ctggttcgga gcgcatccga aaccgtccag ggccgggtgc tcatcgtcga
     3601 caaagccgga ttggtcctga gcgacagcgc cagcgcggaa acggcgggaa gcgattacag
     3661 ctcgagaccc gagattgcgg gagcccttca ggggagcagc gaacagttcg agcgcaccag
     3721 cgacacactc ggcgagagga tcctcgcaac tgccgacccg atccaggccg gcaatcgggt
     3781 gaccggtgcc gtgcggatca cccagagtgt cgatgaggtt catgccgcca tcaacaagtc
     3841 tctgcttggc ctcggactgc tcgccctgct cgttctggct tttgccctgc ttgtcgcagc
     3901 cgtgatcgcc aaccagatct cgcgacccat ccgaaggctg gaagtgagcg cacgccattt
     3961 cgccagcggg gaagtcgagg agaaggccga gatcttcggg agccgcgagc agcgctcact
     4021 ggccagatcg ttcaacgaga tgacgtcgag ggtgaagagg ctgctcaaga gccagagtga
     4081 ttttgtcgcc agcgcatcac accagttgcg gacaccgttg acagggctcc ggctcagggt
     4141 ggaggcgctc agggacgacg cagaaaacga ggagtccagg gctgaacttg actcgggcct
     4201 ggctgaaatc gacaggctct cgagaatggt cgacgagctg cttcttctca gccgggccgg
     4261 ggaggtcgac gtgccgggcg aggatctgga cctaggcgag atcgcacgcg aagcccgcgg
     4321 acgctggaac agggcctccg ggggccggac gctggtcctg accggagccg accggaagga
     4381 catcgacgct gtgttctcgg cccgggccga tcttgaccgc atcctcgacg tgctgatcga
     4441 gaacgccatc cactattcgc ccgaggaaag tgttgtcgag atccaggtcg ttccagccgg
     4501 catccgggtt ctcgacgagg gatcgggtat cgatcccgag gaacgggagg cggtcttcca
     4561 gcgctttgag agaggccggt caggccggga aggcaggaag gggaccggac ttggtctggc
     4621 tatagccagg gaactcgcca ttccatggaa agccaaaatc agcgtggaac ccaggcagcc
     4681 ggcagggacc attgcttcgg tctctttcgc gaagaggaca gactcagaaa gctccgggtc
     4741 gtgatgtttt gcctgctcct aacctttggc catctacgct ggttgaaatg accgacagga
     4801 aagaagccat acccggggag tggggccgct ggaggtggat tgccctcctg gcctgcggca
     4861 tcgtccttgc ggttgccatc gccctcgtgg caggaagcct cgcccatcag ccggtcgggc
     4921 tctccggcga gcccgtatct gccggaagcg ccctggtgcc tgcgaacgtc acgcgaatcg
     4981 aaagcggagg cagcgggcag aagacggggg ctttgaaacg gtcagccggg gaaacggaag
     5041 gaaaatcgac cgatgagccg tcaggccagt ccggaccggt cacttccggc ggttcgtcac
     5101 aacccgaaac tcagagcagc atcggcgaag gcacgggcgg ctccggggat gatggtggcg
     5161 gagaagccga agacgactga tccggttcga tttgccggtt gtaccggctg ttaatgtgcg
     5221 gcacatgagg atcagaacgg caggggccgt acttctggtg gccggtgcga cagtggccgg
     5281 ctgcggtggc ggtggcggtg gcggagaagc tctcgagtcc gactatgcgg atgggacgga
     5341 ggaagcggcc gtggtcaaga ccgtgagcga tttcaatatc gccttcgcca acaaggacgg
     5401 ggagaaggcc tgcagctacc tgaccagcgg cggccagaag aacatggaaa acgcggtcgg
     5461 tgacctgccg ccggacaaga gctgtgaaga cctggtgtcg tcgcgcctcg agcacggccg
     5521 gatcgagggc ggcgggatca acaccgggga catgtggaac tcgctttggc ttgatgtggg
     5581 cgatgacgga aaccactttc tgaacagtca cgaccttgac gaggcgatcg tggtcaaagt
     5641 gaatggtcag gtggcgaggc tcaccgcccc gggccggaac ggactgacat atctgctgac
     5701 cgagcatgac ggcgagtggc tcatcgaggc cgtcggggac gagaacttca acggcgagta
     5761 gggcaggggc ctcgcagccc cggtagacgc tacttcgagt cgcctgagga ggaaccgggc
     5821 gggaagtcca gcttggccag ttccttctgg agccaggggg ccatggtcac catgtaggtg
     5881 gccgacacgt ggtacgagtc gcgatagacg agcgcgttgc cgatcacggc cgggcagcgt
     5941 ccgtccgagc agaggatcgg catcgggtcg atgatctgga ccctggtctt ctcggcggcg
     6001 tcgacatcga agcccagctc ggggtggcgc ttcggggcga aggcgcaggt cttcaaattg
     6061 tggaggttct cggccacaca ttcgtggggc agccagggct ggcgcggggc ctgggcctgg
     6121 tcgcggagca ggatcacctt gtcagcgtcc tccttcagct tgcgaaaagt ccttgccatg
     6181 ccgtcaacca ggtagggctc gctgtcgatg cggctgagtt gcttgccatt cttcttgagg
     6241 ctgtagacgg cctgggtgga agtggcgacg acgaccaggt cgggatgttc ctccttgatc
     6301 cgctcaagcg aattctcccg ccactcatcg cagtacttgg tcagtttcac gtccgcgatc
     6361 acgcaattgg cccgggaaag accggtgatc ttccagccct tctccttgcc gatcgcgtcc
     6421 aatgccggca tgtacatcat tgaatgcgag tcaccgaaaa ggacgaccga gacatcgccg
     6481 tccgggtccc cgtaggtgca atcgcccgac tcggttcgcg gtccgagcag caggcagccg
     6541 tcggcgtaca tctggccgcg gtcttcccgg gcgtgaagcg ggttcggctt gatcttgtcg
     6601 accgaattga ccagtggcac atgcccgttc ttgaccacag cggcgccggc gacctggtcc
     6661 tggtcgatga tgtccacgtt gatccggttg gaaccgagcc agagcccgaa agccgcggcc
     6721 gcgatcgagc agacgccgcc gatcgccagg gcgcggttcg gcttgatctt gaggatcctg
     6781 gcccgccgga ccgggtcctc gagcagggtg tggctgatct gggtcgggat ccacgagagg
     6841 agcgtggcga cgaccagcca cttccaggtc aggttgtcga agatggcgag ggcgaaaacg
     6901 acgaacggcc agtgccagag gtaccaggaa taggagatgt tgcccaggta ctggaagggg
     6961 cgggtcgaga ggatcctggt ggtgatgccg acgccggagg cgccgaccac gagcagggcg
     7021 accgtcgcca gcacggggta gaggccgttc gacccgggga agggggtgga ggccgacatc
     7081 gtgtaagtcg cccagccgag gacgcccacc gcgatcaccc cgatgaccgt tgccagcgag
     7141 gccggcatgc cgatcttctt cggcaggatc atcgccagca cggcgccgaa ggcgagctgc
     7201 caggcccggg cgatggtcga gaagaacgcg gcttcggggt tcacctgggt gaagtggatg
     7261 ctgtagatca gcgaagcgat cgcgaccggc acgatgaaga tcagcacgat gcctttggtc
     7321 cgacggcgga acttcgccgc gagcgcaccg gcggccatca ggatcagggg ccacacgagg
     7381 tagaactgct cttccaccga aagcgaccag tagtgctgga ccggactgtg gatgccgtca
     7441 gcggcagcga aatagtcgac gccctggatg atgaaatgcc agttgacgaa atagagcgcg
     7501 gcggcaaaga cgtcgccacc gatttcgacc tggcgggcga gcgagaagac gaacagggtc
     7561 acgatgcaga tgaagaccag gaccagggcc gccatcggga gaagtcgctt ggcgcggcgg
     7621 gcgtagaagt tccggatcga gaccttgccg gtcttctcga cctcgcgcac gacgaggccg
     7681 gtgatcagga agccggagag gacgtagaag acgtcgaccc cgatgaagcc gcccttggcc
     7741 cagggaatcg aggcgtggca tgcaagcacg agggttaccg cgatcgcgcg gattccctgg
     7801 atgtcgggcc ggaagttgga gcccgcttct tttgccgtgg gggagccgct ttctctggcg
     7861 gcgacctcgg caccgccaag agccatcggc gcaggctacc ggttcatggt ggcgcggtgc
     7921 ccgctcagcg ggggtccctc aggccttgat ccagagggcg tcatcaccga gcggaacccg
     7981 ctgaacctct ccctcgccga gcaggtgaac gagcctgcgc tcggcgtcga tcccgcc
//
LOCUS       DAOTIP010000009         7697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_11403_length_7697_cov_3.603638, whole genome shotgun sequence.
ACCESSION   DAOTIP010000009 DAOTIP010000000
VERSION     DAOTIP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11403_length_7697_cov_3.603638"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..608)
                     /locus_tag="P5138_00390"
     CDS             complement(168..608)
                     /locus_tag="P5138_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59064.1"
                     /translation="MRIPKKIPSSALALLAAGLLIAGCGTDKQESTTLKLETFTSPKG
                     NIGCIADSTMVRCDIAKHSWNVKPDPKCQLDYGNGLAVADKHGYVVCAGDTTMNNGPS
                     VPAGNINLVSPFECQTNEAGDGMHCENVNTGHGFDLSPESYSTF"
     gene            complement(580..3183)
                     /locus_tag="P5138_00395"
     CDS             complement(580..3183)
                     /locus_tag="P5138_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRV59065.1"
                     /translation="MATTTAAPASTAVPSKKDIERASEFAREPLLLNGDGPESLVPGT
                     ARRRAFDAAIAELDAGLTEPSIEWRRNFSLLLGLERLVGQEPPTLKDGAELNPHQVDA
                     LSGTLTELAAEQQVGAIDEIETLEVLEEEGDTPLPEQADEEEPAEEDDGIGEADGDEE
                     DDEAEVIDETVSEDEEPQDWVDRSDEEDEEDEEVAEAADDPGAARRFWFEHATGAGKT
                     VAAVGFIEATRTGGVLILTHRRNLVDQFIGEISDRGYKDRLRPPLLDGRDEADGPVTV
                     ETYQWFVRNAKRVSNAYTVIICDEAHTALGEKTSACIRAWPGPVYIGMTATGALIARH
                     VADLFPTQTSRFDLAQAARRGVIAPLRCLRIPPGPGVRTIAKVPLRKGEVDQDFDQEE
                     LAKLLDQAPFNMAIADLYKSRFRKAPGVLYTAGVQHAKNVAAAFQEFGINAKAVSGET
                     PKRELSETLAAFERGEVDVLCNAQLLAEGWNSPRATICMHLAPTASKRIYQQRVGRVT
                     RRSPNKEAGLVIDFVHPATTHDESIVTLHSLLDRDVYRGGAIVVGPVRRGRGRKVRVE
                     RRIVPVSDDPQRRIEVLERDIWRIAVENLDYAEQHVWAALAGARVTTQGWRRAKAMLQ
                     HDRTKELRRRYLITCVQRNRNAQLRVKALGEIASLKDPEAFDDAIDIVGTWSRDEKRA
                     GTKVLLQALVDKRIGRRDQAQAWVWRLASYTRELHEEYAVQRWPETKRLLGLLVNSAG
                     RAHGKNARKLVQAARKQDRRLAVSLLAAAVAHTPEGEEVLRGARMRMARKPSAVAREL
                     LRNFPKGGKKRRRKKNKKKNQAENPEVENTETTEATTEGEETENAEVESPGTNEEESS
                     GNHADSEEDSE"
     gene            3341..3931
                     /locus_tag="P5138_00400"
     CDS             3341..3931
                     /locus_tag="P5138_00400"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRV59066.1"
                     /translation="MKTAVHRHLLVFALLSVTCVGLVLAGCGDSGEDPSPAPDYAKKL
                     AGAPAPLASLYEQANELLPGGTDAFDERIKGLHGYPAVVNVWASWCGPCRAEFPVFQK
                     TAAAMGKKVGFLGVNAQDNDDAANTFLSTHQVPYPSYTDPDADIAHDLGATHGYPATA
                     FYDAEGNLTYTKSGPFENQAELEDAIRQYAINGDSG"
     gene            3973..4590
                     /locus_tag="P5138_00405"
     CDS             3973..4590
                     /locus_tag="P5138_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRV59067.1"
                     /translation="MEISDPTLVYLLVLLGLTGIGIEAVTPGGFVPAILGIVALVFGV
                     IGMVDIAPTAIGVGLLLLAIVLFMAAVALHLYRPLSIAGVVSLAASGIWMFDRDTDPT
                     SIIAVTLASVVLGLFMLFVIERAGQTRESPVRYGPEDLVGMSGDVRVPLRPEGQVFVD
                     GGLWQARLSDPQARLGIGEKVTVAAIEGLTLVVDPRLETTQGDHL"
     gene            4590..5432
                     /locus_tag="P5138_00410"
     CDS             4590..5432
                     /locus_tag="P5138_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRV59068.1"
                     /translation="MEVIGTLIVILAIFFLIVLFSAIKVLREYERGVIFRLGRLIDQK
                     GPGLILLIPFIDRMVKVDLRTVTLNIPPQEVITRDNVPTSVNAVCYFRVVDSNRAITD
                     VENYLIATSQISQTSLRAVLGKAELDEILAERERLNESLQKIIDEQTEPWGVKVTTVE
                     IKDVEIPDAMQRAMARQAEAERERRAKIINSEGEYQAAEKLTDAADIISRNPASLQLR
                     YLQTLLEISGNQNSTVIFPLPMDLISAFQQTLGNLGNPGEGSDGGIQPTIGGSKPDDL
                     SSEP"
     gene            5440..6510
                     /locus_tag="P5138_00415"
     CDS             5440..6510
                     /locus_tag="P5138_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59069.1"
                     /translation="MSSPEVRIDQLTGGRVLVAPGRSERPDDFSPFQWHPAGHDGCPF
                     CEGNEAATPPEIEASRPGGGDADSPGWVTRTVPNLYPAVQPREGGEAEDGRKVADRAG
                     AESSAFSSPADPLLASGRGSEPDMFASRPAHGSHEVIINSPFHLPSLAEMEEESLAAA
                     VAAWRSRMRAHGDSAYVQLILNQGPDSGASLEHSHAQLGAMEFVPPSVARERERVGAY
                     RERTAGASLLGEILREEIRRGERLVAIDDEVALICPWASRHPYEMRIIPRRPTSRFEE
                     DEGGAAMLGRAVRALTGLFGNPCQLNLWVRTAPRGVEHFHWHVDLIPRLEPASAFEMA
                     TGVDINIVTPEAAAGTLREAID"
     gene            6524..7225
                     /locus_tag="P5138_00420"
     CDS             6524..7225
                     /locus_tag="P5138_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59070.1"
                     /translation="MPIDNRPFPRFVADASRHGIPHGRFAERLAAAFREACGEIEDLP
                     AGTAIPDEFTWFPERAWSGRVWIPVSGDSEAVLEEGGEPEPIELFGFVSFVQPEGGDP
                     ADFKASADFTDVVPADNPDWTIDLSDEVIGTWRGEQGREAAMTLVWGRSLVRDAFAVT
                     AELDEVTVDQDPLFGGQGGRADRFTLIAPDALKNYGDDAYLEIKLWTSRGKQVARESL
                     YEIEEPETPEENPEV"
     gene            7238..>7697
                     /locus_tag="P5138_00425"
     CDS             7238..>7697
                     /locus_tag="P5138_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59071.1"
                     /translation="MKNQQRVEELRELIDRESEGPVLSVFCRTDPRDPANSSETPAWS
                     IALKNGLSHVTGFHEGDHGKEQSVKKLCAEAERRLTVASAAERGRSVALFLGSGGSID
                     SFTTFQIPVRQDYVTIDSGPVVWPMLDVIDRGQRTGIALLSHDRIRLLEWW"
BASE COUNT         1207 a         2532 c         2550 g         1408 t
ORIGIN      
        1 cggctaccgg gtcttcgccg cttcctcggc cgacggcaag cgttcgattg ccttcgtcgc
       61 caacgcccag atcctcgccc acacccaggg caacccgaac gccccccagg tcagcgcgaa
      121 gatccgccgc gcccaggtcg ccgcggtctg ttacgccctg aagtagttca gaaagtggag
      181 tagctctccg gggagagatc gaagccgtgg ccggtgttga cgttctcgca gtgcatgccg
      241 tcgcccgctt cgttggtctg gcattcgaac gggctgacca gattgatgtt tcccgcgggg
      301 accgacggac cattgttcat ggtcgtatcc ccggcacaga ccacataacc gtgcttgtca
      361 gcgacggcga ggccgttgcc gtaatcgagc tggcatttcg ggtccggctt gacgttccag
      421 ctgtgcttcg cgatgtcgca gcggaccatc gtgctatcgg cgatgcagcc gatgttgccc
      481 ttcggggaag tgaaagtctc cagcttgagc gtcgtgctct cctgtttgtc ggtaccgcaa
      541 ccggcgatca ggagaccggc ggccagcagt gccagggcgc tactcggaat cttcttcgga
      601 atccgcatgg tttccggagg attcttcctc atttgtcccg gggctctcca cctcggcgtt
      661 ttcagtctct tcgccttcgg tggttgcttc cgtggtctcc gtattctcga cctccggatt
      721 ctccgcctgg ttcttcttct tgttcttctt gcgacgacgc ttcttgccgc ccttggggaa
      781 gttccggagc agctcgcggg caacggcgga gggcttcctc gccatcctca tccgggcgcc
      841 gcgaaggacc tcttcgcctt ccggtgtgtg ggcgaccgcc gcggcgagca gcgaaacggc
      901 caggcgacgg tcctgcttgc gggcggcctg gaccagcttc ctcgcgttct tgccgtgggc
      961 gcggccggcc gagttgacca gcaggccgag caggcgcttg gtttccggcc agcgctggac
     1021 cgcgtactcc tcgtgaagct cgcgggtgta tgaagcgagt cgccagaccc aggcctgggc
     1081 ctggtcgcgc cggccgatcc gcttgtcgac cagggcctgg agcaggacct tggtgccggc
     1141 ccgcttctcg tcccggctcc aggtaccgac gatgtcgatc gcgtcatcga aagcttcggg
     1201 gtccttcagg ctggcgatct cgccgagcgc cttgaccctg agctgcgcat tgcggttgcg
     1261 ctggacgcag gtgatcaggt agcggcggcg cagttccttg gtccggtcgt gctggagcat
     1321 ggccttcgcc cggcgccagc cctgtgtggt gacccgggcg ccggccaggg cggcccagac
     1381 atgctgctcg gcgtagtcga ggttctcgac cgcgatccgc cagatgtccc tttccaggac
     1441 ttcgatgcgg cgctgcgggt cgtcggagac cgggacgatc cgtcgctcca cccggacctt
     1501 gcggccgcgg ccacgacgga ccgggccgac cacgatcgcc ccgccgcggt aaacgtcgcg
     1561 gtcgagcagg ctgtgcaggg tgacgatcga ctcgtcatgg gtggtcgccg gatgtacgaa
     1621 gtcgatcacc agtccggctt ccttgttcgg agagcgacgg gtcacgcggc cgacccgctg
     1681 ctggtagatc cgcttcgagg cggtcggggc cagatgcatg cagatcgtgg cgcgggggga
     1741 gttccagccc tcggccagca actgggcgtt gcagagcacg tccacctcgc cccgttcgaa
     1801 ggcagcgaga gtctcggaga gttcacgctt cggggtttcg ccggagacgg ccttggcgtt
     1861 gatgccgaat tcctggaagg cggcggcgac gttcttggcg tgctggacgc cggctgtgta
     1921 gagcacgccc ggtgccttgc ggaagcgtga cttgtagagg tcggcgatcg ccatattgaa
     1981 cggcgcctgg tcaagcagct tggccagctc ctcctgatcg aagtcctggt cgacctcgcc
     2041 cttgcggagt ggcaccttgg cgatggtgcg gacgcctgga cccggcggga tgcgaaggca
     2101 gcgcagcggg gcgatcacgc cccggcgggc agcctgggcg aggtcgaagc gtgaagtctg
     2161 ggtggggaag aggtcggcga cgtgacgggc gatcagggcg ccggtcgcgg tcatgccgat
     2221 gtagaccggc ccgggccagg cccggatgca ggccgaagtc ttttcgccga gggcggtgtg
     2281 ggcctcgtcg cagatgatca ccgtgtaggc gttggagacc cgctttgcgt tgcggacgaa
     2341 ccactggtag gtctcgaccg tgaccgggcc gtcggcctcg tcgcggccgt cgagcagcgg
     2401 cgggcgcagc cggtccttgt aaccgcggtc ggagatctcc ccgatgaact gatcaaccag
     2461 gttgcggcga tgggtgagga tcaggacgcc gccggtgcgg gtcgcttcga tgaagccgac
     2521 cgcggcaacc gtcttgccgg ctccggtggc atgctcaaac cagaagcggc gggcagcacc
     2581 cgggtcgtcg gcagcctcgg cgacttcctc gtcctcttca tcttcttcgt cggacctgtc
     2641 gacccagtcc tggggttcct cgtcttcgga gacggtttcg tcgatgacct cggcttcgtc
     2701 gtcttcctcg tcgccgtcgg cctccccgat cccgtcgtcc tcttccgcgg gttcttcttc
     2761 gtcggcctgc tccggaagcg gcgtgtcgcc ctcttcctcc agcacttcca gggtctcgat
     2821 ttcatcgatt gccccgacct gctgctcggc cgcaagttcg gtcagcgttc cggagagggc
     2881 gtcgacctga tgggggttca gttccgcccc gtccttcagg gtgggtggct cctgcccgac
     2941 caggcgttcc aggccgagca gcagtgagaa gttccggcgc cattcgatcg aaggttcggt
     3001 caggccggcg tcgagttcgg cgatcgcggc gtcgaacgcc cggcggcggg cggtgcccgg
     3061 aaccagcgac tccggaccgt ctccgttcag caggagtggc tcacgcgcga attcggacgc
     3121 acgctcgatg tctttctttg aggggaccgc agttgatgcg ggtgctgcag tagtcgtggc
     3181 caactctagc cttcgcaagt gatccgaacc tcgggtatcg acccggcccg gatgagtagg
     3241 tgatcagagt ttccgacggc gacgctggac ggacggacgc cggattcctc cgtgatccgc
     3301 ggaacggcgg atcggtctcc tgtatttaat cattactcga atgaaaacgg ctgttcacag
     3361 acatctgctt gttttcgccc tgctttcggt cacctgcgtg ggactggttc tggccggctg
     3421 cggtgacagc ggcgaggacc cttcaccagc ccccgactac gcgaagaaac tggccggcgc
     3481 acccgcccct ctggcctcgc tctacgagca ggcaaatgag ctcctgccgg gcggtaccga
     3541 cgccttcgac gagcggataa aggggcttca cggctacccg gccgtggtca acgtgtgggc
     3601 ttcctggtgc ggcccctgcc gggcagagtt tccggttttc cagaagaccg ctgcggcgat
     3661 gggcaagaag gtcggcttcc tcggggttaa cgcccaggac aacgacgacg cggcgaatac
     3721 cttcctctcc actcaccagg tgccctaccc gagttacacc gaccccgatg cggacatcgc
     3781 ccatgacctg ggggcgaccc acggttatcc ggccacggcg ttttacgacg ccgagggcaa
     3841 cctgacctac acgaagtcgg gtccgttcga gaaccaggcg gagctcgaag acgcaatccg
     3901 ccagtacgcg ataaacggcg actccggctg aacggttccc tgcccgctgg gcaggaccga
     3961 tacgctcggt tcgtggagat cagcgacccg acactcgtct acctcctggt ccttctcggt
     4021 ctgaccggga tcgggatcga ggcggtcacc ccgggtggct tcgtgccggc gatcctcggg
     4081 atcgtggctc tggtcttcgg cgtgatcggc atggtcgaca tcgccccgac cgcgatcggc
     4141 gtcggactgc tgcttctggc aatcgtgctg ttcatggccg ccgtggccct tcacctctat
     4201 cgccctctct cgatcgccgg cgtagtctcg ctggccgcct ccggaatctg gatgttcgac
     4261 cgggataccg acccgacctc gatcatcgcg gtgaccctgg cttcggtcgt gctagggctt
     4321 ttcatgctct tcgtgatcga aagggccggt cagaccaggg aatcgccggt ccgctacggg
     4381 ccagaagacc tggtcggcat gtccggcgac gtcagggtcc cgctgcgacc ggaagggcag
     4441 gtcttcgtcg acggcggact ctggcaggca cgtttgtccg atccccaagc ccgtcttggt
     4501 atcggcgaaa aggtcaccgt agccgcgatc gaaggcctga cgctggtcgt cgacccccgc
     4561 ttggaaacca ctcaaggaga ccacctgtaa tggaagtcat cggaaccctg atcgtcatcc
     4621 tggcgatctt tttcctgatc gtcctgttct cggcgatcaa ggtactgcgt gaatacgagc
     4681 gaggggtgat tttccggctc gggcgtctga tcgatcagaa gggccccgga ctgatcctgc
     4741 tgatcccctt catcgaccgc atggtgaagg tcgacctgcg gacggtcacc ctgaacatcc
     4801 cgccgcagga agtgatcacc cgcgacaacg ttcccacctc ggtcaacgcg gtctgttact
     4861 tccgggtcgt cgattcgaat cgcgcgatca ccgacgtcga gaactacctg atcgcgactt
     4921 cgcagatttc ccagacatcg ctccgggcgg tgctcggcaa ggccgaactg gacgagatcc
     4981 tggccgagcg cgaacgtctg aacgagtcgc tccagaagat catcgacgag cagaccgaac
     5041 cctggggcgt caaggtcacc acggtcgaga tcaaggacgt cgagattccc gacgcgatgc
     5101 agcgggcgat ggcccggcag gcggaggccg agcgtgaacg ccgggcgaag atcatcaact
     5161 ccgaaggcga gtaccaggcg gccgagaagc tcaccgacgc ggcagacatc atcagccgca
     5221 accctgcctc gctgcagttg cgctacctgc agacgctgct cgagatctcg ggcaaccaga
     5281 attcgaccgt gatcttcccg ctgccgatgg acctgatctc cgccttccag cagaccctcg
     5341 gcaatctcgg caatccgggc gaggggtcgg acggcggaat tcagccgacc atcggaggca
     5401 gcaagccgga cgatctttca tcggaaccct gaacccggtt tgagttcgcc tgaagtcagg
     5461 atcgaccagc tgaccggcgg ccgggtgctg gtcgcgcccg gccgttcgga gcggccggat
     5521 gacttcagcc cgtttcagtg gcatccggcc gggcatgacg gctgcccttt ctgcgagggc
     5581 aacgaagccg cgacgccacc cgagatcgag gccagccgcc cgggcggcgg cgatgccgac
     5641 tcgcccggct gggtcactcg gacggtgccg aacctctacc cggcggttca gccccgggaa
     5701 ggcggggaag cggaggacgg ccgcaaggtt gccgaccggg ccggggccga atcgagcgcc
     5761 ttctccagcc cggccgaccc gctgctcgcc tccggccggg gttccgagcc ggacatgttc
     5821 gcctcccgtc cggcccatgg cagccacgaa gtgatcatca actcgccttt tcaccttccc
     5881 tcgctggcgg aaatggagga ggagtccctt gcggccgcgg tggcggcgtg gcgcagccgg
     5941 atgcgggctc acggggattc ggcctacgtc cagctgatcc tcaaccaggg tcccgattcg
     6001 ggcgcgtcac tggagcacag ccacgcccag ctcggggcga tggagttcgt cccgccttcg
     6061 gttgcacgcg agcgggaaag ggtgggtgcc taccgggagc gaaccgccgg agcgagcctg
     6121 ctcggcgaga tcctccgcga ggagatccgc cggggcgaac gcctcgtcgc aatcgacgac
     6181 gaggtcgcat tgatctgtcc ctgggcttcc cgacatccct acgagatgcg gatcattccc
     6241 cgtcgtccca catcccgttt cgaggaggac gaaggcgggg ccgcgatgct gggccgggcg
     6301 gtccgggcgc tgaccggact cttcggcaat ccctgccagc tgaacctgtg ggtccggacc
     6361 gcgccgcggg gagtcgagca cttccactgg catgtcgatc tcattccccg gctcgaaccg
     6421 gcttcggcct tcgagatggc gaccggggtg gacatcaaca tcgtcactcc cgaagccgcg
     6481 gccggaaccc tgcgcgaggc gatcgactga gtaggctgcc ccgatgccga tcgacaatcg
     6541 acccttcccc cgtttcgtcg ccgatgcctc ccgccacggc atcccccacg gccgcttcgc
     6601 cgagcgtctc gccgcggcgt tccgggaggc ttgcggcgag atcgaggatc tccccgccgg
     6661 caccgccatc ccggacgagt tcacctggtt cccggagcgg gcctggtccg gccgggtctg
     6721 gataccggtt tccggtgact ccgaggcggt cctggaggag ggcggcgaac cggagccgat
     6781 cgagctcttc ggtttcgtca gcttcgtcca gcccgagggc ggtgacccgg ccgacttcaa
     6841 ggcttcggcc gacttcaccg acgtggtccc cgccgacaac ccggactgga cgatcgacct
     6901 ctccgacgag gtgatcggca cctggcgtgg cgagcagggc cgcgaagccg cgatgaccct
     6961 ggtctggggg aggtcgctgg tccgtgatgc cttcgccgtc accgccgaac tcgacgaggt
     7021 cacggtggac caggacccgc tcttcggagg ccagggtggt cgggcggacc gcttcaccct
     7081 gatcgccccc gatgcgctca agaactacgg tgatgacgcc tacctcgaga tcaagctctg
     7141 gacctcacgc ggcaaacagg tggcccgcga gtcgctctac gagatcgagg aacccgaaac
     7201 tccggaggaa aaccccgagg tttgatttga tcggggtatg aagaaccagc agagggttga
     7261 ggagcttcgg gaactgatcg atcgtgagtc cgaagggccg gtgctctcgg tcttctgccg
     7321 gaccgatccg agggatcccg ccaacagcag cgagacgccg gcctggtcga tcgcgctgaa
     7381 gaacggcctc agccacgtca ccggctttca cgagggtgac cacggcaagg agcagtcggt
     7441 gaagaagctc tgcgccgagg ccgaacgccg cctgaccgtc gcctcggcgg ccgagcgggg
     7501 acgaagcgtc gccctgtttc tcggatcggg gggcagcatt gactcgttca cgacctttca
     7561 gatcccggta aggcaggact acgtgacgat cgattcgggc ccggtggtct ggccgatgct
     7621 ggacgtgatc gaccgcggcc agcgcaccgg catcgctctg ctcagccacg accggatccg
     7681 cctgctcgaa tggtggg
//
LOCUS       DAOTIP010000010         7025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_12771_length_7025_cov_3.112912, whole genome shotgun sequence.
ACCESSION   DAOTIP010000010 DAOTIP010000000
VERSION     DAOTIP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12771_length_7025_cov_3.112912"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="P5138_00430"
     CDS             complement(<1..213)
                     /locus_tag="P5138_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59072.1"
                     /translation="MLVALLGLVALAFVLPASALASPTHFVKVYKVEKQLDLDGHGYA
                     HDHVYCNPGDYAVDGMWRVDNVDQANP"
     gene            complement(368..2530)
                     /locus_tag="P5138_00435"
     CDS             complement(368..2530)
                     /locus_tag="P5138_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59073.1"
                     /translation="MTQAAGTTGLKSRLVLLITFMSAFGFSIEAEAEANLEPVVVSQV
                     KDADPGAGDGDPLQGGNNADQAGGVDLDGILIYDSNDGFTNGEDHGQELWRSDGTDEG
                     TYLLKDISVGAPNSSLPSWYTEFKGMVYFTALKDSLTGIEMWRTDGTTEGTELFFDFN
                     EVIFESGAPVNLTVVGDTMYFLANSDGLGGDAFVSKELWKTDGTVAGTTMVKDIKSSS
                     TNAAYNLTPYGDSFFFTYKDPTDGKELWISDGTESGTHMVKNIGPGSADGLPYPSSYQ
                     DENDFYVFNGLLFFEANDGSGTELWRSDGTGPGTFQFKDINGTAGGSMGFGGQFTELN
                     GELFFSWDFGMQKTDGTVGGTVPLTIPGGTNHPLHTVRIGNEIFFIGTSSRIFKSDGT
                     NAGTELVVQLDPPNVIQNVTAFDGNLYFVYDDGVHGKELWRNDGTELGTERLTDINEA
                     GDSLVNELTAVGDRLFFRGDDNDATGIELWQVLADRTPPETTIDFGPAEGENLAIDEA
                     SFTFSSSEPGSTFNCVLDGGTPEPCNSGSKTYVGLGVGDHTFEVAAVDPAPFGNVDPT
                     PATRNFSFGVEDPDPDPDPDPDPVPPDKSSPTLRLKAKKRQFSARRVVVKATCVNETC
                     TLKAKGKIKVKVRKASGKFRTRKFNLKLVKRGAAKGKTVTLRVKLPNKARRLVKRAMR
                     NRKITRIRVTVWATDEAGNTRHLTRKVKVLKKKRKLRG"
     gene            complement(2670..3107)
                     /locus_tag="P5138_00440"
     CDS             complement(2670..3107)
                     /locus_tag="P5138_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRV59074.1"
                     /translation="MSSADDNSAVAREVWQLMSDLVLDNQRRRKVTEVTGMSFGRTRA
                     VRRVAKRSMQMGELADLLGMERPNLTTLINDLEEKGLVIRKADPRDRRSRLVEATAKG
                     KRVARKAEAILKEPPDRLVGLDVEDLETLRRCLAEASAPGGSE"
     gene            complement(3120..4559)
                     /locus_tag="P5138_00445"
     CDS             complement(3120..4559)
                     /locus_tag="P5138_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRV59075.1"
                     /translation="MTNTGTLSRNRKIGVLMICSMSLFMVGLDVTAMNVALPAIGRDM
                     QADVSWLQWTVSAYTVMMASFLMFAGSMADRLGRKRTLITGMSVFSAASLLCSFAPNV
                     ELLVAFRVLQGVGASMLNPVAMSIITNTFVEPRERAQAIGVWGAVFGVSMALGPVVGG
                     GLVSTLGWDWIFLMNVPLGAAAIGLISCFVPESKAETPRRFDPVGQVLMVGLLASVTF
                     GIIEGPSAGWESPLILGAFSVAVVSLAGLLAYEPQRDQPLIDLRFFRSIPFSASITLS
                     VAAFATFGGFLFLNTLYLQEVRGLAPLEAGLAVVPLALMTVIMSPVSGRITGREGPRK
                     PLLIASATTIISCGMLVGIGAGTPFAWLVVAYVIFGTGFGLVNAPITNAAVSGMPVSQ
                     AGVAAAVATTSRQFGQAVGVAIVGAIVTSHPGSSTGADLAMASHGAWWALAASGVGML
                     VLGLVATSSTARESARRTRRELDPDAAVA"
     gene            4720..4851
                     /locus_tag="P5138_00450"
     CDS             4720..4851
                     /locus_tag="P5138_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59076.1"
                     /translation="MTFLASIPAVLLYGPMLDDPEFILGAGELEGLPILLAIFGGTG"
     gene            4893..5738
                     /locus_tag="P5138_00455"
     CDS             4893..5738
                     /locus_tag="P5138_00455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59077.1"
                     /translation="MFDPDSYREQSLRTWGEMAPGWRDRNDWMMSITGVVNDWLVEHA
                     DPQPGQRFLEVACGAGDLGCQVADRVGDEGHVISTDFAPEMVEVAGNRGNERGLSNLE
                     HRVLDAEQMDLEDDSVDGMVCRWGYMLMADPAAALRESKRVLRDGGNLAFAVWAAPDR
                     NPWAAIPGMTLVQRGHMDPPEPGAPGIFGMADPDRIRDLVTGAGLAEPELEEIPFEFT
                     YPDFDDLWDSIVRIAGPLANAIKAQPEDEQVATREAIKENFASFQGEDGSYAAAAVTW
                     GVLTG"
     gene            5955..>7025
                     /locus_tag="P5138_00460"
     CDS             5955..>7025
                     /locus_tag="P5138_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59078.1"
                     /translation="MATTTDASGKTAPYEPIAVHHLSVPAGYSGESEPDWLPDGKHLV
                     AVVASPDLSAPQLAITRLDGSHPKCVTCGLDTSVVPGAPDGAAYGKPLPFPDGKRLMV
                     RLGLRPGGGGSATQNFTYGVVECKPSLADCQERSLAPIIMPGGGIDQGTQNREGRLSP
                     DGRWFGWTEFTLHGTAMSIGLLAKYGDQYDLKNVRVLNPSDPLGTNSAAWRAQSGYFE
                     LKNFTSDGKSVLYSTTYDADNFDTWRVDLRTGKRTRITRGIDWEEDVSTSPDGGSYLQ
                     FSSRGFDRMPIFALMPRPPFIDFAFFSWVGRYELNQVNRKCLLEPWLMDTGGERGTYF
                     GQPVDPRPGTGWDNRTLGQWSPD"
BASE COUNT         1255 a         2248 c         2179 g         1343 t
ORIGIN      
        1 cggattcgcc tgatcgacgt tgtccaccct ccacatgccg tcaacggcat agtccccggg
       61 attgcagtac acgtgatcgt gcgcgtaccc gtgaccgtcc aggtcgagct gcttctcgac
      121 cttgtagacc ttgacgaaat gagttggcga ggcgagcgcg cttgccggca atacgaaagc
      181 cagcgcaaca agccccagga gcgcaacgag caacctccgg cccgagcgcg acgacgatgt
      241 acctgtctcc atttaattcc ctccgttcgg acagccagtc ctctttgcct gtgccagccg
      301 tcactgaaga acgtaaccgc agcattcgaa aaatgcaaac gaccgggcga gatcgcccga
      361 ttggacgtca gccgcggagc ttgcgctttt tcttgagcac cttcaccttg cgggtcagat
      421 gccgggtgtt tccggcttcg tcggttgccc agaccgtgac ccggattctg gtgattttcc
      481 ggttcctcat cgccctcttc accagtcgcc tggccttgtt aggcaacttg accctgaggg
      541 tgaccgtctt gcccttcgca gcgccccgct tgacgagttt caggttgaac ttcctggtcc
      601 tgaatttccc gcttgccttg cgtaccttca ccttgatctt gcccttcgcc ttcagggtgc
      661 aggtttcgtt tacgcaggtg gccttgacca cgactcggcg ggcgctgaac tgacgcttct
      721 tcgccttcag ccgcagggtc gggctgctct tgtcgggcgg taccgggtcc ggatcggggt
      781 ccgggtctgg gtcggggtcc tccaccccga agctgaagtt cctcgtcgcg ggtgtgggat
      841 cgacgtttcc gaacggggcc gggtcgacgg cagcgacctc gaaggtgtga tcgcccacgc
      901 ccagcccgac ataggtcttg ctccccgagt tgcagggttc cggggttccg ccatcgagca
      961 cgcaattgaa ggttgaaccg ggctcgctgg agctgaaggt aaagctcgct tcgtcgattg
     1021 ccaggttctc gccttccgcg ggcccgaagt cgatcgtggt ctcgggcggt gtgcgatccg
     1081 cgagcacctg ccacagctcg atgcctgtgg cgtcgttgtc atcaccccgg aagaagagcc
     1141 ggtcccccac cgcggtcagc tcgttgacca gcgaatctcc ggcttcgttt atgtcggtta
     1201 gccgttcggt cccgagttcg gtgccgtcgt ttcgccacag ctccttgccg tggactccgt
     1261 cgtcgtagac gaagtagagg ttgccgtcga aggcggtcac gttctggatc acattcgggg
     1321 gatccagctg gaccacgagc tcggtgcccg cgttcgttcc gtccgacttg aagatcctgc
     1381 tcgaggttcc gatgaagaag atctcgttgc cgatccgcac cgtgtgaagg ggatgattcg
     1441 tcccgccagg aattgtgagg ggcacggttc caccgaccgt tccgtctgtc ttctgcattc
     1501 cgaaatccca cgagaagaac aactcgccgt tcagctcggt gaactggccg ccaaagccca
     1561 tgctcccgcc ggcggtgccg ttgatgtcct tgaactggaa ggtgccgggt ccggttccat
     1621 cggagcgcca gagttccgtg ccgctcccgt cgttggcctc gaagaagagc agcccgttga
     1681 agacgtagaa gtcgttctcg tcctggtagc tcgacgggta gggcagccca tcggccgatc
     1741 cggggccgat gttcttgacc atgtgggtac ccgactcggt cccgtccgag atccagagct
     1801 ccttgccgtc ggtcggatcc ttgtaggtga agaagaagct gtccccgtag ggggtcaggt
     1861 tgtaggcggc attcgttgag gaagacttga tgtctttgac catcgtcgtt ccggcgaccg
     1921 tcccgtcggt cttccacagt tccttgctga cgaaggcatc acctcccaat ccgtccgaat
     1981 tggcgaggaa atacatggtg tcgccgacga ctgtcagatt gacgggcgcc ccgctttcga
     2041 agatgacctc gttgaaatcg aagaagagct cggtaccctc ggtggttccg tcggttcgcc
     2101 acatctcgat cccggtcaac gaatccttga gggcggtgaa gtagaccatc cctttgaact
     2161 ccgtgtacca gctgggcagg ctcgaattcg gtgccccgac ggatatgtcc ttgagaaggt
     2221 aggtcccttc gtcggtgccg tccgaacgcc agagctcctg accgtggtct tcgccgttgg
     2281 tgaatccgtc gttggagtcg tagatgagga ttccgtcgag gtcgacgccg ccggcctggt
     2341 cggcattgtt tccgccctgc agcggatcgc cgtctccggc tccgggatcg gcgtccttta
     2401 cctgcgagac gacaaccggt tcgaggtttg cctcggcctc ggcctcgatc gaaaagccga
     2461 acgcggacat gaacgtgatc aggaggacca gcctggactt cagtccggtc gtgcctgctg
     2521 cctgagtcat gttggaactt cctcctcagc cctgcccggg cttcgatccc atttcgccgg
     2581 gtccggaacc tcgaatcccg gtccccttga ccgtatcagg gcctcacgca cccgctgtga
     2641 attttcgcgc cactcccggg acctttgccc tactcgcttc cacccggagc acttgcttca
     2701 gccagacagc gccggagagt ctcgagatct tccacgtcca acccgaccag tctgtcgggc
     2761 ggctccttca ggatcgcctc ggccttcctc gctaccctct tgcctttcgc ggtggcttcg
     2821 acgagcctgc tcctgcgatc ccgcgggtct gccttgcgga tgaccagccc cttttcttcg
     2881 agatcgttga tcagggtggt caggttgggg cgctccatgc cgagcaggtc cgccagctca
     2941 cccatctgca tcgaccgctt cgcaacacgg cgcaccgccc gggtccgtcc gaacgacatt
     3001 cccgtgactt cggttacttt gcgccggcgc tggttgtcga gcacgaggtc cgacatcaac
     3061 tgccagacct cgcgcgcaac cgccgaattg tcgtcagcgg aactcatgtg ggggcgagct
     3121 tatgcaaccg cggcatccgg gtcgagttcc cgtctggtac gccgagcgga ctcgcgggcc
     3181 gtgctactgg tagcaacgag tccgagcacc agcatcccga cgccggacgc agcgagagcc
     3241 caccatgcgc cgtgacttgc catggccagg tcggcgccgg ttgaacttcc agggtgggaa
     3301 gtcactattg cgccgactat cgcgacaccc acagcctggc cgaactggcg ggacgtagtg
     3361 gccactgccg ccgctacgcc cgcctgggaa acgggcattc cggaaaccgc cgcgttggtg
     3421 atcggcgcgt tcaccagtcc gaagccggtt ccaaagatca cgtaagcgac caccagccac
     3481 gcaaagggag tcccggcccc gatcccgacc agcatcccgc aggaaatgat ggtggtcgcc
     3541 gacgcgatca gcaggggctt gcgcgggccc tcgcggccgg tgatgcgccc cgagaccggg
     3601 gacatgatca cggtcatcag ggccagggga acgacagcga ggccggcttc gagcggagct
     3661 agcccgcgta cctcctgcag gtagagcgtg ttcaggaaaa ggaaaccccc gaaggtcgcg
     3721 aaggcggcaa ccgaaagcgt gatcgaagcg gagaatggga tcgagcggaa aaacctcagg
     3781 tcaatcagcg gctgatcccg ctgtggttcg taggccagca gcccggccag cgagacgacg
     3841 gcaaccgaga aggcgcccag gatcagcggc gattcccagc cggcgctggg gccctcgatg
     3901 attccgaagg tcaccgacgc cagcaggcca accatcagga cctggcccac ggggtcaaag
     3961 cgccggggcg tctcggcctt cgattccggg acgaaacagc tgatcaaccc aatcgcagca
     4021 gcccccagcg ggacgttcat caggaagatc cagtcccagc cgagggtgga aaccagaccc
     4081 cctccgacca ccgggcccag ggccatcgag acaccgaaca ccgcgcccca gaccccgatc
     4141 gcctgggccc gctcccgtgg ttccacgaag gtgttggtga ttatcgacat cgcaaccggg
     4201 ttgagcatcg aggccccgac cccctgaagc acccgaaagg cgaccagcag ttccacgttc
     4261 ggggcaaagc tgcagagcag ggatgcggcc gagaagacgc tcatgccggt gatcagcgtc
     4321 cgcttgcgtc ccagccggtc tgccattgat ccggcgaaca tcaggaagct ggccatcatc
     4381 accgtgtagg cgctcacggt ccactggagc caggaaacgt cagcctgcat gtctctcccg
     4441 attgcgggga gagcgacatt catcgcggtc acgtcgagtc cgaccatgaa caggctcatc
     4501 gagcagatca tcaagacgcc gatcttcctg ttgcgactga gggtgcccgt gttggtcatg
     4561 gaatgcctcc tgtcaattac ttacgtttct ataactatta tattatcata aataatactg
     4621 aacagacctg gtgtccctgt cgattggggc gatccacgtc tcaagcaagg gtcctgcccc
     4681 gggcgccgga ttgccctgct cgctggcccg ttcttcgcgg tgactttcct cgcgtcaatt
     4741 ccggccgtac tgctctacgg cccgatgctc gacgatcctg agttcatcct tggcgccggg
     4801 gaattggaag gattgccgat cttgttggcg attttcggtg gcaccggata gcttcggttg
     4861 ggggcaattt gaccgactgg agatgtgagg caatgtttga tcccgattcc taccgcgagc
     4921 agagtctcag gacatggggc gagatggcgc ccggctggcg cgaccgcaac gactggatga
     4981 tgtcgatcac cggcgtggtc aatgactggc tcgtcgagca cgctgatcct caacccggcc
     5041 aacgcttcct cgaagtcgcc tgtggcgcgg gtgacctggg atgccaggtg gccgaccggg
     5101 tcggggacga aggccacgtg atttccaccg atttcgcgcc cgagatggtc gaggttgccg
     5161 gcaaccgcgg gaacgagagg gggctctcga acctcgaaca ccgggtactg gacgccgagc
     5221 agatggacct cgaagacgac agtgtggatg gcatggtctg ccgttggggc tacatgctga
     5281 tggccgatcc cgcggctgcc ctgcgtgagt cgaagcgcgt gcttcgtgac ggtggcaatc
     5341 tggccttcgc ggtctgggct gcgccggatc gcaacccgtg ggcggcgatt ccggggatga
     5401 ccctggtcca gcggggccac atggaccctc cggaaccggg tgctcccggc atcttcggga
     5461 tggccgaccc cgaccggatt cgcgacctgg tgaccggcgc gggactagcc gagcccgaac
     5521 tggaagaaat ccccttcgag ttcacctatc ccgacttcga tgatctctgg gattcgatcg
     5581 tgcgaatcgc cggaccactg gccaacgcaa tcaaggccca acccgaagac gagcaggtag
     5641 cgaccaggga agcgataaag gagaatttcg ccagtttcca gggcgaggat ggctcctacg
     5701 ccgctgccgc cgttacctgg ggagtcctga ccggctgatc gaacggaagc cccgagggcg
     5761 caccctccgg gggcaactgt cccttcagtc cggggaacgg caactgcgga cgaagaaaaa
     5821 gtacgtgtgc gtacgctatt gtcgtggtgg cgggtcggcc gccatgggaa aagacccttg
     5881 aggaggggta cgtgaagcac atccgttcga tgttgcgagc aggcgcagca ggcattccgg
     5941 ttcttctggc gttgttggcc acgacaacgg acgcaagcgg caagacggcc ccctacgagc
     6001 cgattgccgt gcaccacctc tctgttccgg ccggatactc gggtgaatcg gaaccggact
     6061 ggcttccgga cgggaagcac ctggtcgccg tggtcgcatc ccccgatctc agcgcaccgc
     6121 agctcgcgat cacccgcctc gacggttccc atcccaagtg tgtgacctgc ggcctggaca
     6181 cgagtgtggt tcccggcgcc cctgacgggg cggcttacgg caagcccctc ccgttcccgg
     6241 acggcaagcg gctgatggta cggcttggcc tgagacccgg cggcggaggc agcgccaccc
     6301 agaacttcac ctacggagtc gtggagtgca agcccagcct ggccgattgc caggagcggt
     6361 cgctggcgcc gatcatcatg ccgggaggcg gaatcgacca ggggacccag aaccgggaag
     6421 gccgcctctc gccggacggt cgctggttcg gctggaccga gttcaccctt cacgggactg
     6481 cgatgtcgat cggcctgctg gcgaagtacg gcgaccagta cgacctgaaa aacgtgaggg
     6541 tcctcaatcc ttcggacccg ctcggcacca attctgcggc ctggcgagcg cagtccggtt
     6601 atttcgaact gaagaacttc acttccgatg gaaagtcggt tctctactcg accacctacg
     6661 acgccgacaa cttcgacacc tggcgggtgg atctccggac cggaaagcga acccgcatca
     6721 ccagggggat cgactgggag gaagacgttt ccacctcccc ggatggcggc tcctacctcc
     6781 agttcagctc acggggcttc gaccgaatgc cgatcttcgc cctgatgccg aggccgccgt
     6841 tcatcgattt cgcattcttc tcctgggtcg gccgttacga gctcaaccag gtcaaccgca
     6901 agtgcctgct ggagccgtgg ctcatggaca ccggaggcga gcggggaacc tacttcggcc
     6961 agccggtgga cccgcgaccg ggcacgggct gggacaaccg tacgctcggc cagtggagcc
     7021 cggac
//
LOCUS       DAOTIP010000011         6845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_13182_length_6845_cov_3.097349, whole genome shotgun sequence.
ACCESSION   DAOTIP010000011 DAOTIP010000000
VERSION     DAOTIP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13182_length_6845_cov_3.097349"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="P5138_00465"
     CDS             <1..651
                     /locus_tag="P5138_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRV59079.1"
                     /translation="SSGTILTLEMATAMNRSVANILFAGFGSPATGGGGDQEQRPYRS
                     MNAQDAAIQLAYADSVVVVPGYGLAVAQAQHTVKEMADELNKKGVTVNYAIHPVAGRM
                     PGHMNVLLAEADVPYDQLKEMDEINPELPQTDVCVVIGANDVTNPAAKTDESSPIYGM
                     PIIEVDEAQQVLVLKRSMSAGFAGIDNDLFYNEKTAMVFGDAKQTVQDIVTELGNL"
     gene            complement(742..1635)
                     /locus_tag="P5138_00470"
     CDS             complement(742..1635)
                     /locus_tag="P5138_00470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59080.1"
                     /translation="MTASSELPYPPFELANRVIELPDDDFAGYVSYEMAGRQTRDELL
                     ELLPDEFSFEGRSMLDFGCGAGRTLRQFHDEAASARFVGCDIDEESIDWMNGNLCPPL
                     EAVRSRVDPPLPFEDCTFDFIWAISVFTHLTDNSAAWLLELHRLLKPGGLLMASYMGE
                     LNSEEVSGEPWDEDHVGMNVLRHSQGWDLGGPMVMMSDWWVDAHWGRAFRILDRRFTG
                     GQTWPLMEKRDVSLTPEQLMEPSDDPREFAALRHNVKQLQAEVEEVREALASAENQNT
                     GSKQGFRSTVGRRLRRSTSGQ"
     gene            1704..2519
                     /locus_tag="P5138_00475"
     CDS             1704..2519
                     /locus_tag="P5138_00475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRV59081.1"
                     /translation="MERNRILLGDNLDLMAGIEDESIQLVYADPPFNTGRRQARRSVR
                     TIADEEGDRTGFGGRRYRTESSGESSYADVFDDFAAFLEPRLREVHRLLDPAGTLYLH
                     IDYREAHYVKVILDGIFGRDCFLNEIIWAYDYGGKSKRRWPAKHDTILVYVKDPDRYY
                     FDSEEVDREPYMAPGLVTPEKAAKGKLPTDVWWHTIVPTNGSEKTGYPTQKPEGILRR
                     IVTASSRPGDLCLDPFCGSGTLGAVCRELGRDYVLIDENPEAIEVTRGRLGEG"
     gene            complement(2524..3132)
                     /locus_tag="P5138_00480"
     CDS             complement(2524..3132)
                     /locus_tag="P5138_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV59082.1"
                     /translation="MSLPVIEPVCLEGEGVTLEPIREDLAEDLWEAAQDPEIWHWTAH
                     LNHSREFFDDWVASGIAGWQTGERNTFVTSTRRSGSGDPARVVGSSSFLYYRPDDDAV
                     EIGSTWLNPSAWGSGTNTEAKLLMMTHAFETLGCVRVEFKTDRRNERSRAALAALPAR
                     FEGIHRKHSRIFGVGRRDSAFYSVIDDEWPEVRAKLESRIAG"
     gene            complement(3140..3418)
                     /locus_tag="P5138_00485"
     CDS             complement(3140..3418)
                     /locus_tag="P5138_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HRV59083.1"
                     /translation="MADRNKPSSPPTAAKKEVQWKWWAFGVAVLLLLIVVLQNSQDVE
                     FKVLFLVDTSAPLFILLLLGALIGAIIGYTAPILRRHRHNTRKEYGKE"
     gene            3523..4404
                     /locus_tag="P5138_00490"
     CDS             3523..4404
                     /locus_tag="P5138_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59084.1"
                     /translation="MRGQSTVEWVGLITLVACVMAGLTAAGIRLPAAGLAHSVSTKIL
                     CAASLSRSCAGDGSLEVAYGPELAGLVRSGAPTLMYGKDMLGLPVDFRTCRSPWCADG
                     SGEGEVSESTAGEPVTLFTRVVEGPEDSTYVQYWAYYPESASLRGAPFLEGKGYHRND
                     WESMQIRVEADGTVSERASSHSGYNHSRSSANWGSDLGWEFLTDAAEAVGLREKGGWG
                     EATGRYLIAGGSHAGNVATSADDTDYPSYTPARLVRLVPLESVRNGPLARPADFDPIT
                     PPWEKQVWTDPESEGTG"
     gene            complement(4444..5286)
                     /locus_tag="P5138_00495"
     CDS             complement(4444..5286)
                     /locus_tag="P5138_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59085.1"
                     /translation="MSQDFEAAFNQMDTRGADDFTVPQGEEWFIDTVAVAGKHLGAPA
                     VPTAFRVAFFEDNGELPGSEIAAFESNGGPYPAKGQSATTAIPLGVGPQLGPGEYWVS
                     VQAIMDSHIDVPNEDASRWFWGVKPAGHIGSSAVFENPGAGFNEFTCTSFAPLKDCSS
                     NPGIVDADFAFRLDGATSVTAECAAATNAVATANGSLTTAKSALSRAKAALTKAQKAV
                     KKAQSKLKKAKGKRAKLKAKTVLRKSKKKATAATASVKKAKKKVGSANAALSTAKTNQ
                     SSVC"
     gene            complement(5511..6446)
                     /locus_tag="P5138_00500"
     CDS             complement(5511..6446)
                     /locus_tag="P5138_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRV59086.1"
                     /translation="MASNRWGLTLPLPGVSLADTKDFVQLAEAEGYTDLWTGETGGPD
                     GFTPLALSAAWTEKVRLGTGIVGVFQRGPALLAQQAAALTDASSGRFVLGIGASSDRM
                     VEGWNQIPFVKPLSKVEATVDFLRVALAGERTDTGFKLETAPTHETPIVLAALRGKML
                     ELAVNKADGAFTNFLPLAGLPKVTDGISAAGDDFELLCRFFCIAGEKEQVEGLARFMF
                     SSYITVPVYANFFRWLGYGEQIEPMIEAWNNKDRQGAAAAAPWELIEDTFLLGSPEQI
                     RERLDAYVEGGITLPVITPITTPDNHAALIKALAP"
     gene            6583..>6845
                     /locus_tag="P5138_00505"
     CDS             6583..>6845
                     /locus_tag="P5138_00505"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HRV59087.1"
                     /translation="MGVISHDVRSGSKPDRNLALELVRVTESAALAAARWIGRGDKIA
                     ADGAAVDAMRILLDTVPMDGIVVIGEGEKDEAPMLYNGEQIGDG"
BASE COUNT         1218 a         2195 c         2209 g         1223 t
ORIGIN      
        1 tcctcgggca ccatcctcac cctcgagatg gcgaccgcga tgaaccgctc cgtcgccaac
       61 atcctcttcg ccggcttcgg ctcgcccgca accggtggcg ggggagacca ggagcagcgt
      121 ccctatcgtt cgatgaacgc ccaggacgcc gcgatccagc tcgcctacgc cgactcggtc
      181 gtggtcgtgc ccggttacgg actcgccgtc gcccaggccc agcacacggt caaggagatg
      241 gccgacgagc tgaacaagaa gggcgtcacg gtcaactacg cgatccaccc ggtcgccggc
      301 cgcatgcccg gccacatgaa cgtcctgctg gccgaggccg acgtgcccta cgaccagctc
      361 aaggaaatgg acgagatcaa ccccgagttg ccgcagaccg acgtctgcgt cgtgatcggg
      421 gccaacgacg tcaccaaccc ggccgccaag accgacgagt ccagcccgat ctacggcatg
      481 ccgatcatcg aggtcgacga agcccagcag gtgctggtcc tcaagcgctc catgtccgcc
      541 ggtttcgccg ggatcgacaa cgatctcttc tacaacgaga agaccgccat ggtcttcggc
      601 gacgccaagc agacggtgca ggacatcgtc acagagctcg gcaacctcta gccccgcgcc
      661 agaaatcgga ccgaggctcc agcacccacc tgcccgaagc aggtgaaaaa cacttggttt
      721 cgtcctggca cggaacgagt ttcactgtcc ggaagtagac cgcctcaggc ggcgaccgac
      781 ggttgacctg aagccttgct tcgaccccgt gttctgattc tcggcggaag cgagggcttc
      841 ccggacttct tctacctccg cctgaagctg cttcacgttg tggcggaggg cggcaaactc
      901 ccgcgggtcg tcagagggtt ccatcagctg ttccggggtg agcgaaacgt cccgcttttc
      961 catcaggggc caggtctggc cacccgtgaa cctgcggtcc aggatccgga aggcccggcc
     1021 ccagtgcgcg tcgacccacc agtcggacat catcaccatc ggcccgccga gatcccagcc
     1081 ctggctgtgt cgaagcacgt tcatgcctac gtggtcttcg tcccagggct cacctgaaac
     1141 ctcttccgag ttcagctcgc ccatgtagga cgccatgagc agcccacccg gcttcaacag
     1201 gcggtgcagc tcgagtagcc aggcggccga gttgtcggtc aggtgggtga acacggagat
     1261 cgcccagatg aagtcgaagg tgcagtcctc gaaaggaaga ggcggatcga cccgggagcg
     1321 aacggcttcc aggggaggac agaggtttcc gttcatccag tcgatgctct cttcatcgat
     1381 gtcgcatccg acgaaccggg cagaggctgc ctcgtcatga aactggcgca gtgtgcgccc
     1441 ggccccgcag ccgaagtcga gcatgctccg tccctcaaac gagaattcgt ccggcagcaa
     1501 ctcgagcagt tcgtccctgg tctggcggcc cgccatctcg taactgacat agccggcgaa
     1561 atcgtcatcg ggaagctcta tcacccggtt ggcgagctcg aagggcggat atggcagttc
     1621 gctgctggcg gtcaccagga ggaaactacg cgattgacgc cttgccggga cagaccggga
     1681 cggagtgatc tagcgttcac cacatggagc gcaaccggat cctgctgggc gacaatctcg
     1741 acctgatggc cgggatcgag gatgaatcga tccagctcgt ctatgcggat cctcccttca
     1801 acaccggacg tcggcaggcc cgccgttcgg tcaggacgat cgccgacgag gaaggcgatc
     1861 gcacgggctt cggcggacgc aggtaccgga ccgagtcctc cggcgagagc tcctacgccg
     1921 atgtcttcga tgacttcgcg gcattcctcg agcctcgtct gcgagaggtc caccgtctgc
     1981 tcgacccggc ggggaccctc tatctccaca tcgactatcg cgaagcccac tacgtgaagg
     2041 tgatcctgga tggaatcttc ggccgggact gcttcctgaa cgagatcatc tgggcctacg
     2101 actacggcgg caagtcaaag cgccgctggc cggccaagca cgacacgatc ctggtctatg
     2161 tgaaggaccc ggaccgctac tacttcgact cggaggaagt cgatcgtgag ccctacatgg
     2221 caccgggcct ggtcacgccg gagaaggcgg cgaagggcaa gctgccgacc gacgtctggt
     2281 ggcacacgat cgtgccgacc aacggcagcg agaagaccgg ctatccgacc cagaagccgg
     2341 agggaatcct gcggcggatc gtcaccgcct ccagccggcc cggggacctc tgcctcgacc
     2401 cgttctgcgg ttccgggacc ctgggggcgg tctgccggga actcggtcgc gactacgtcc
     2461 tgatagacga gaatccggaa gcgatcgagg tcacgagggg gaggctgggg gaaggttgaa
     2521 gggtcagccg gcgatgcgcg actcgagctt cgcccggact tccggccatt cgtcgtcgat
     2581 gacgctgtag aaggccgagt cccgtcggcc gaccccgaag atcctggaat gcttgcgatg
     2641 gattccctcg aaccgggccg gtaacgccgc cagggcggcc cgggaccgtt cgtttcggcg
     2701 gtcggtcttg aactcgaccc tcacgcagcc aagcgtctca aaggcatgag tcatcatcag
     2761 gagctttgcc tccgtattgg tcccgctgcc ccaggccgat ggattgagcc aggtcgaccc
     2821 gatttcgacc gcatcgtcgt caggccggta gtagaggaaa ctgctgctgc cgaccaccct
     2881 cgccggatcg cccgatccgg aacggcgggt gctggtcacg aaggtgttgc gttcacccgt
     2941 ttgccagccg gcgattccgc tggcaaccca gtcgtcgaaa aactctcggc tgtgattcag
     3001 atgcgccgtc cagtgccaga tttccgggtc ctgggcggct tcccagagat cctcggccag
     3061 gtcctcccgg atcggctcca gcgtgacacc ctcgccttcg aggcacaccg gctcgattac
     3121 gggaagcgac acgttgcagc tattccttgc cgtattcctt gcgggtgttg tggcggtggc
     3181 gacggaggat cggggccgtg tagccgatga tcgcgccgat caatgccccg agtagcagca
     3241 ggatgaagag cggggcggag gtgtcgacca ggaacaggac cttgaactcg acgtcctggg
     3301 agttctgaag taccacgatc agaagcagca gcaccgcgac accgaaagcc caccacttcc
     3361 actggacttc cttctttgcg gcggtcgggg gagaggaggg tttattgcga tcggccatgt
     3421 ctggactcta tatctgccgc ctgtcacgga atcgttgcac cggtaaccca cgcttgagtt
     3481 ctttccggcg acgatcatcg ggtggcgtca ggtcataaat ctgtgcgtgg tcagtcgacc
     3541 gtcgaatggg tcgggttgat cacgctcgtc gcctgtgtga tggccgggct taccgctgcg
     3601 gggatcaggc ttccggcggc cggactcgcc cattcggtct cgacgaaaat cctctgtgct
     3661 gcatccctgt cccggtcctg cgccggtgac ggatcactgg aggtcgcata cggtccggaa
     3721 ctcgccggac tggtccgttc gggagcgccg accctcatgt acgggaagga catgctcggc
     3781 ctgcctgttg acttccggac ctgtcgctcc ccatggtgcg ccgatggatc gggggaagga
     3841 gaagtaagcg aatcgactgc cggcgagccg gtaaccctct tcacgcgggt ggtcgaaggc
     3901 cccgaagaca gcacctacgt tcagtactgg gcctactacc cggagagtgc ctcgctgagg
     3961 ggagcaccgt tcctggaggg caaggggtat caccggaacg actgggaatc gatgcagatc
     4021 cgggttgaag ccgacgggac ggtcagcgag agggcctcgt cccacagcgg ttacaaccac
     4081 agccgatcat cggccaactg gggctccgat ctcggctggg agtttctcac cgacgcagca
     4141 gaggcggtcg gactgcgcga gaagggtggt tggggcgagg caacgggccg ctatctgatc
     4201 gccggcggca gccacgccgg caacgtggca acgagtgccg atgacaccga ctatccctcg
     4261 tacaccccgg ccaggctggt ccgtctggtg ccgctggagt cggtcaggaa cggcccgttg
     4321 gcccggccgg cggacttcga cccgatcacg ccgccctggg agaagcaggt ctggaccgac
     4381 ccggagtccg agggaacggg ctgaaccggg tgacccggcc cagcccgtcg accccggaaa
     4441 ctctcagcag acggaactct gatttgtctt tgcggtgctc agtgccgcgt tggcgctgcc
     4501 aacctttttc ttggccttct tgaccgatgc agtcgccgcg gtcgccttct tcttcgactt
     4561 cctcaggacc gtcttggcct tcagctttgc cctcttgccc ttcgccttct tcagcttcga
     4621 ctgggccttc ttgacggcct tttgggcctt ggtcagggcg gccttcgccc tggacagggc
     4681 gctcttggct gtggtgagac ttccgttggc cgtggccacc gcgttggttg ccgctgcgca
     4741 ttccgcggtg accgaagtcg ctccatcgag ccggaacgcg aagtcggcgt ccacgatgcc
     4801 cgggttggaa gaacagtcct tcaggggggc gaagctggtg catgtgaatt cgttgaaacc
     4861 ggctccgggg ttctcgaaga ctgccgatga gccgatgtgg ccggccggct tgacgcccca
     4921 gaaccagcgg gaagcatctt cattcggaac gtcgatgtgg gaatccatga tcgcctgaac
     4981 tgaaacccag tactcgccgg gccccagctg aggtcctacc ccgaggggaa tcgcggtcgt
     5041 tgcgctctgc cccttcgccg gatagggccc cccgttcgac tcgaaggcgg caatctcgct
     5101 tcccggcagt tcaccgttgt cttcaaaaaa ggcaaccctg aaggcggtcg gaacggccgg
     5161 cgcgccgagg tgcttgccgg ctaccgcgac ggtatcgatg aaccactctt caccctgcgg
     5221 gaccgtgaag tcgtcggccc cgcgtgtgtc catctggttg aacgctgctt cgaaatcctg
     5281 tgacaccagt gagtcttgga acccgccccc gccgacgttg tcgtacagga cgtcggcccg
     5341 tgcctggtca gcgaaaaacg cgaggctggc aaagatcagg cctaccagcc aacctgttct
     5401 acgacgaaac aaatcatccc tttcgtaacg cccagggcag ttcccccgga ctgcgtggcg
     5461 caattactac cacggcgcca acccgagaac aagagttatg gctcaatgtt tcagggggcc
     5521 agggccttga tcagggcggc gtggttgtcc ggggtggtga tcggcgtgat cacgggcagg
     5581 gtgatgccgc cttcgacgta ggcgtcgagc cgttcgcgga tctgttcggg gctgccgagc
     5641 aggaaggtgt cctcgatcag ctcccagggg gcggccgcgg cggcgccctg gcggtccttg
     5701 ttgttccagg cttcgatcat cggctcgatc tgttccccgt agccgagcca gcggaagaag
     5761 ttcgcgtata ccggcaccgt gatgtagctg gagaacatga agcgggcgag cccttcgacc
     5821 tgctctttct ccccggcaat gcagaagaag cggcagagca gttcgaagtc atcgcccgcg
     5881 gcgctgatgc cgtcggtgac cttgggcagt ccggccagcg gcaggaagtt ggtgaaggct
     5941 ccgtcggcct tgttcacggc cagctccagc atcttgccgc gcagtgctgc gagaacgatc
     6001 ggggtctcgt gggtcggtgc ggtttccagc ttgaatccgg tgtcggttcg ttcaccggcg
     6061 agcgcgaccc gcaggaagtc gaccgtggct tcgacctttg aaagcggctt gacgaagggg
     6121 atctggttcc agccctcgac catccggtcg gaggaggcgc cgatgccgag cacgaaacgc
     6181 cccgaggatg cgtcggtcag ggccgctgcc tgttgggcca gcagggccgg gccccgctgg
     6241 aagacgccga cgatgccggt gccgagcctg actttttcgg tccaggcggc gctcagggcc
     6301 agcggagtga agccgtcggg cccgccggtt tcaccggtcc agagatccgt gtaaccctcg
     6361 gcctccgcaa gctgcacgaa gtccttggtg tcggccagtg agaccccggg cagggggagg
     6421 gtcagacccc atcgattgct cgccatgggc cgcagcatat cccgctcctg ttgcagtcac
     6481 ggtcccgcag ccgcggtggg tcggatttcc ggccgggggg ccggaaatcc gacccaccgc
     6541 ggcgggaatc gccattttcc ctcgcgcagt agcctcccgc gcatgggtgt tatctcgcac
     6601 gacgttcgat ccggatcaaa gcccgaccgc aacctggccc tggagctggt tcgcgtgacc
     6661 gaatcggcgg cgctggccgc cgcaaggtgg atcggccggg gtgacaagat tgccgccgac
     6721 ggcgccgccg tcgacgccat gcgcatcctg ctcgacacgg ttccgatgga cgggatcgtg
     6781 gtgatcggcg agggcgagaa ggacgaagcc ccgatgctct acaacggcga gcagatcggc
     6841 gacgg
//
LOCUS       DAOTIP010000012         6829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_13223_length_6829_cov_2.939179, whole genome shotgun sequence.
ACCESSION   DAOTIP010000012 DAOTIP010000000
VERSION     DAOTIP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_6829_cov_2.939179"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5138_00510"
     CDS             complement(<1..701)
                     /locus_tag="P5138_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRV59088.1"
                     /translation="MLSDYEQTRFAYDGLERRLFVRGDGPGIVVMHEVPGIYDAVVAF
                     ADRLVAEGYRVYMPELVGEAGRPFSGGYAASSILRACIAREFHVLSARGSSPITDWLR
                     ALARHAHAECGGRGVGCIGMCLTGNFGLAMMVDEVVMAPVLSQPSLPFPIGAARRRGL
                     HVTDEALGVIRERVRRDGCRVLGLRFSNDPMCPPERFARLREELGPGFEGIEIDSAPG
                     NAHGIPTSAHSVVTR"
     gene            complement(759..1592)
                     /locus_tag="P5138_00515"
     CDS             complement(759..1592)
                     /locus_tag="P5138_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRV59089.1"
                     /translation="MGALALVFVAWPLVSSAQTPGIVSTDGPGDEAVWREAERAAVRA
                     EEAAEAARAFADSLAPASEGGATRAADAGSAGGGADRRALEQAVERAEAAAADARLAA
                     NAVERAERRSRRTSRSGPYLGGVVFYAAEQFDDSVIVKSSAGGGAFVGWRFEEFFAVE
                     VRYEGFAGFDLQGRTSRGEIDGYAITANGRLYPMRGPIQPFLTVGVGGIRLRGESVRD
                     SGARSRFVEDDVVLRGGAGVDLIVNEHLAVNLEAAWLGPGDDLSNLEMALFGGGLTYR
                     F"
     gene            1940..3835
                     /gene="ilvD"
                     /locus_tag="P5138_00520"
     CDS             1940..3835
                     /gene="ilvD"
                     /locus_tag="P5138_00520"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV59090.1"
                     /translation="MQGYRSRTTTFGRNMAGARALWRATGMTDADFTKPIIAIANSFT
                     QFVPGHVHLHDVGQRVKQVIDAAGGWGVEFNTIAVDDGIAMGHGGMLYSLPSRDLISD
                     SVEYMVNAHCADAMVCISNCDKITPGMLNAALRLNIPAVFVSGGPMEGGTATLVDGTV
                     RKRVNLISAIADAVADEVSDEDIEIIEENACPTCGSCSGMFTANSMNCLTEALGLSLP
                     GNGSTLATHTARRELYEAAGRTAVEIAGRYYEEDDESVLPRSVATRDAFENAMTLDIA
                     MGGSTNTVLHLLAAAREADVDFEMSDIDELSKRVPCICKVAPNGHFLMEDVHRAGGIP
                     AILGELRRGGLLNENVHTVHSPDIDTWLGSWDVRGPKPSEVALDLFHAAPGCKRSPQA
                     FSQSERWESLDVDPVEGCIHDVEHAYSKDGGLAILYGNIAERGCVVKTAGVDESILTF
                     SGPAFVVESQDDAVDSILSGRVKSGDVVVIRYEGPRGGPGMQEMLYPTSYLKGLGLGK
                     ECALLTDGRFSGGTSGLSIGHVSPEAAAGGAIALVETGDEIVIDIPDRSITLAVDDEA
                     LAARREALEASGGYQPVDRDREVSPALRAYAMMATSADTGAVRDVEGVERLVNAARLL
                     DSEQIGS"
     gene            3903..4667
                     /locus_tag="P5138_00525"
     CDS             3903..4667
                     /locus_tag="P5138_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRV59091.1"
                     /translation="MLRIGHKGADALVPGNTIASFVKAVEVGVDAIEFDVLWTEDGHP
                     SIDPGKRSELVVAHDWHAAAAAERKGGKLTLETALEAFKQPPLDRVRINLDIKLPGRD
                     DEVFDAVVRHRLVERTSVSTMEVSTLRRLGELKREDLIESGMSLGWTVPKVTRDWLSM
                     PKVIRPALAMGLVSVRVRMPGQVRRGLQELEVDSIWAFFGVVTPRLVETIHDAGKKLN
                     VWTVDDPDRIDSLTAMGVDGICSNDPRLLYTRMLGV"
     gene            complement(4664..5506)
                     /locus_tag="P5138_00530"
     CDS             complement(4664..5506)
                     /locus_tag="P5138_00530"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HRV59092.1"
                     /translation="MRRIKAGWQLTKKSWRVLSDSPGLVRFPLIGGLIAFLIAIVLIG
                     PGLYFFEDGTPVPGAILIAVGTYLCAFVTYYFAVALAHNADRQMHGETPEFGDGIALA
                     SSRMGEIAGWAFVATVVMSIIRAIQERFGIAGAIVGGLAGAAWGILTFLAVPVIALEG
                     TGPIGTIKRCAHLVKSRWGEQIAGTIAIGGIVFLVGFLPAILFIVAGVALWTTTGFGG
                     AILIGVGVLILVAAALIQQSLATIFGVALYRYVAGKEAVGGFTEEELQSAVRLKRGAE
                     PAAI"
     gene            complement(5516..>6829)
                     /locus_tag="P5138_00535"
     CDS             complement(5516..>6829)
                     /locus_tag="P5138_00535"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRV59093.1"
                     /translation="LFRGGDSSQEKLDERHAETAARMVATLGQMKGAAMKIGQFASFI
                     DIDFIPEEYREIYQEQLGKLRSEAPAMPWEEVVKVLREEYDGEPIEQLFDSIEHEAFA
                     AASIGQVHRGRLIDGREVAVKIQYPGIAEALESDLKNAGMLVRMARALAPGLDAREVT
                     RELRERVLEELDYEYEAQNQRSFARAYRGHPFIYVPDVVTRLSRRRVLVTEFVEGIGF
                     EEILELDHEQRSRFGEILFRGSFGSIYHLQHFNADPHPGNYLLMEDGRVAFLDFGMTK
                     KLDKEQIELEQLAVDAAVRNDPEGLRDALHNLGFVKKPSKLDAEHLMNHVMSIGGWYM
                     EDREIEINGDRVMKVIESTHDPRSEYFDLMRRESIPADELMGRRMEIGVLAVLGRLGA
                     KRNWHRIMREWVYADPPATDLGKEEWAYFKERGILQVPGIAAPTE"
BASE COUNT         1141 a         2399 c         2215 g         1074 t
ORIGIN      
        1 tcccgggtga cgacgctatg cgccgaggtc gggatgccgt gggcattgcc gggcgcggag
       61 tcgatctcga tcccctcgaa gccgggtccg agctcctccc gcagccgcgc gaagcgctcc
      121 ggcggacaca tcggatcgtt gctgaagcgc agaccgagca cccgacaacc gtcgcgccgc
      181 acccgctccc ggatcacgcc caacgcctcg tccgtcacgt gcagcccccg ccttcgcgcg
      241 gcgccgatcg gaaagggcag cgagggctgc gagagcacgg gtgccatcac gacctcgtcg
      301 accatcatcg cgaggccgaa attccccgtc aggcacatgc cgatgcagcc gacgccgcga
      361 ccgccgcact cggcgtgggc gtgacgcgcg agagcgcgca gccagtccgt gatcggactc
      421 gagccccgcg ccgagagcac gtggaactcg cgggcgatgc aggcgcgcag gatcgagctc
      481 gcggcgtagc cccccgagaa gggacgcccg gcctcgccga cgagctcggg catgtagacg
      541 cggtagccct cggcgaccag tcggtcggca aaggcgacga cggcgtcgta gatgcccggc
      601 acctcgtgca tgacgacgat gcccggcccg tcgccgcgga cgaacagacg tcgttcgaga
      661 ccatcgtacg cgaagcgcgt ctgctcgtag tccgagagca tggctcctcc ttcgatctcg
      721 cggcgggcac tcgcgacgct gcgcgaagcc ggtccgcctc agaagcgata ggtcagtcca
      781 cccccgaaga gcgccatctc gagattggat agatcgtcgc cgggaccgag ccaggccgcc
      841 tcgaggttca cggcgagatg ctcgttcacg atcaggtcga cgccggcgcc accgcgcagc
      901 accacgtcgt cctcgacgaa gcgactccgc gcgcccgagt cgcgaacgct ttcgccccgc
      961 agccggatgc cgcccacgcc gaccgtgagg aagggctgga tcggaccgcg catgggatag
     1021 aggcgtccgt tggccgtgat cgcatagccg tcgatctcgc ctcgactggt ccgaccctgc
     1081 aggtcgaagc ccgcgaagcc ctcgtagcga acctcgaccg cgaagaactc ctcgaagcgc
     1141 cagccgacga aggcgccgcc gccggcgctg ctcttgacga tcacggagtc gtcgaattgc
     1201 tcggcggcgt agaagacgac cccaccgaga tagggccccg agcgcgacgt gcgacgactg
     1261 cggcgctcgg cgcgctcgac cgcgttcgcc gcgagtcggg cgtccgcggc tgcggcctcc
     1321 gcgcgctcga ccgcctgctc gagtgcgcgt cgatcggcgc cgccacccgc cgacccggcg
     1381 tcggcagccc gcgtcgcccc gccctcggac gcgggggcca gcgagtcggc aaaggctcgc
     1441 gcggcctcgg ccgcctcctc cgcccggacc gccgcgcgct cggcctcgcg ccagaccgcc
     1501 tcgtcgccgg gtccgtccgt cgagacgatc cccggtgtct gtgcactcga cacgaggggc
     1561 cacgccacga agaccagcgc gagcgccccc acacagccag cacgccagcc cgaacgacgc
     1621 cgtctataag aagacgacgt cgccagccgc ctgcgacccc gcgtcatccg accggccgcg
     1681 ccgcccgtcg cggcgcgccg ccctcgcatc ccgctcgatc ccatcgccgt ccctccctcg
     1741 agacgcccgt cggcgcgtcc gcggcgaccg agaggcccgg ggagtctcgc gggcttcccg
     1801 cgacggcgcg agtcgcaagc gccctccgcg cccgggtcga tccatcgcgc gccatgggcc
     1861 cggggggtcc agccccccgg ccggccgatc cagccggacc ttcgtgcagc ttcgcgaacc
     1921 cgcccctatc ctcgcgccca tgcagggata tcgcagtcga acgacgacct tcggacgcaa
     1981 catggccggg gcccgggcgc tctggcgcgc cacgggcatg accgatgccg acttcaccaa
     2041 gccgatcatc gcgatcgcca acagcttcac ccagttcgtg cccggccacg tccatctcca
     2101 cgacgtcggc cagcgcgtga agcaggtgat cgacgcggcc ggcggctggg gcgtcgagtt
     2161 caacacgatc gccgtcgacg acggcatcgc gatgggccac ggcggcatgc tctactcgct
     2221 gccctcgcgc gacctgatct ccgacagcgt cgaatacatg gtcaacgccc actgtgccga
     2281 tgcgatggtc tgcatctcga actgcgacaa gatcaccccg ggcatgctga acgccgcgct
     2341 gcggctcaac attccggccg tcttcgtctc cggtggtccg atggagggcg gcaccgcgac
     2401 cctggtcgac ggcacggtcc gcaaacgggt caacctgatc tcggcgatcg ccgacgcggt
     2461 ggccgacgag gtttccgacg aggacatcga gatcatcgaa gaaaacgcct gcccgacctg
     2521 cggctcctgc tccggcatgt tcaccgccaa ctcgatgaac tgcctgaccg aggcgctcgg
     2581 cctctcgctg ccgggcaacg gttccaccct cgcgacccac acggcccgcc gggagcttta
     2641 cgaagcggcc ggccgtaccg cggtcgagat cgccggcagg tactacgaag aggacgacga
     2701 gagcgtcctt ccccgctcgg ttgcgacccg cgacgccttc gagaacgcga tgaccctgga
     2761 catcgcgatg ggcggctcga ccaacacggt gcttcacctg ctggccgccg cccgtgaggc
     2821 cgacgtggac ttcgagatgt ccgacatcga cgagctctcc aagcgggtgc cctgcatctg
     2881 caaggtcgcc ccgaacggcc acttcctgat ggaggacgtc caccgggccg gcgggattcc
     2941 ggcgatcctc ggcgaactgc gccgtggcgg attgctcaac gagaacgttc acacggtgca
     3001 ttcgccggac atcgacacct ggctcgggag ctgggacgtg cgcggcccga agccatccga
     3061 agtcgcgctc gatctcttcc acgccgcacc cggctgcaag cgttccccac aggccttttc
     3121 gcagtcggag cggtgggagt cgctggacgt cgacccggtc gagggctgca ttcacgacgt
     3181 cgagcacgcc tactcgaagg acggcggcct ggcgatcctc tacggcaaca tcgccgagcg
     3241 cggctgcgta gtcaagacgg ccggggtcga cgagtcgatc ctcaccttca gcggaccagc
     3301 cttcgtcgtc gaatcccagg atgacgcggt tgactcgatc ctcagcggcc gggtcaagtc
     3361 gggcgacgtc gtcgtgattc gctacgaagg cccgcggggc ggaccgggca tgcaggagat
     3421 gctttacccg acctcttatc tgaagggcct cggcctcggc aaggaatgcg cgctcctgac
     3481 cgacggccgc ttctccggcg gcacctccgg cctctcaatc ggccacgtgt cccccgaagc
     3541 tgcggccggc ggggcgatcg ccctggtcga gaccggggac gaaatcgtga tcgacatccc
     3601 cgaccgttca atcactctcg cggttgacga cgaggcgctg gccgcccgcc gggaagctct
     3661 ggaggcgtcg ggcggatacc agccggtcga tcgcgaccgg gaggtctcgc ctgctcttcg
     3721 tgcctacgcg atgatggcca cctcggccga caccggtgcg gtgcgggacg tcgagggcgt
     3781 cgagcggctg gtcaacgccg cgcggctgct cgactccgaa cagatcggct cctgacccgt
     3841 cagcttcgtc cgctcctcgg attttcaccg ggagttcacg tggttttcgt agggtttggt
     3901 ggatgctccg tataggtcac aagggcgccg acgcgctggt tcccggaaac acgatcgcct
     3961 ccttcgtgaa ggcggtcgag gtcggggtgg acgcgatcga attcgacgtg ctctggaccg
     4021 aggacggtca tccgtcgatc gatccgggga agcgctccga actggtcgtt gcccacgact
     4081 ggcacgccgc ggcggcagcg gaaaggaagg gcgggaagct gaccctcgag acagccctcg
     4141 aggccttcaa acagccgccg cttgaccggg tccggatcaa ccttgacatc aagctgccgg
     4201 gccgggacga cgaagtcttc gacgcggtgg tcaggcaccg cctggtcgag cgcacctcgg
     4261 tctcgaccat ggaggtttcg actctcaggc gcctgggaga gctgaaacgg gaggatctga
     4321 tcgagtccgg gatgagcctc ggctggaccg tgccgaaggt gaccagggac tggctctcga
     4381 tgccgaaggt gatcaggcct gccctggcga tgggactggt gtcggtcagg gtccggatgc
     4441 ccggtcaggt tcgccgcggg ctccaggaac tcgaggtcga ctcgatatgg gccttcttcg
     4501 gcgttgtgac gccgcgcctc gtcgagacga tccatgacgc cggcaagaaa ctcaacgtct
     4561 ggacggtcga tgaccctgac cgcatcgact cgctgacggc gatgggcgtg gacgggatct
     4621 gttcgaacga tccccgtctg ctctacaccc ggatgctcgg cgtctagatt gcggccggct
     4681 cggcgccccg cttgaggcgg accgcgctct gcagctcttc ttcggtgaag ccgccgaccg
     4741 cttccttgcc ggccacatag cggtagagag cgacgccgaa gatggtggcc agcgactgct
     4801 ggatgagagc agcggcgacc aggatcagga ccccgacccc gatcaggatc gcacctccga
     4861 agccggtcgt ggtccagagg gccaccccgg caacgatgaa gagtatggcg ggcaggaaac
     4921 cgaccaggaa gacgatgccg ccgatcgcga tcgtgcctgc gatctgctcg ccccagcggc
     4981 tcttgaccag gtgggcgcag cgcttgatcg tcccgatcgg gccggtgcct tcgagggcga
     5041 tgaccgggac cgccaggaag gtgaggatgc cccaggccgc gccggcaaga ccgccgacta
     5101 ttgcgccggc gattccgaac cgctcctgga ttgcccggat gatgctcatc acgacggtgg
     5161 cgacgaaggc ccagcctgcg atttcgccca tccggctcga ggcgagagcg atgccgtcgc
     5221 cgaactcggg ggtttcacca tgcatctgac ggtcggcatt gtgggccagg gcgaccgcga
     5281 aatagtaggt cacgaaggcg cagaggtagg tgccgacggc gatcagaatt gcaccgggca
     5341 ccggcgtgcc gtcttcgaag aaataaaggc cggggccgat cagcacgatc gcgatcagga
     5401 aggcgatcaa tccgccgatc agcggaaaac gcaccagtcc cggcgagtcc gagagaaccc
     5461 gccagctttt cttggtcaac tgccagccag ccttgatccg tcgcattgac ccagattact
     5521 ccgtcggggc ggcaattcca gggacctgaa ggatcccccg ctctttgaaa taggcccatt
     5581 cctccttgcc gaggtcggtc gcgggcgggt cggcatagac ccattcgcgc attatccggt
     5641 gccagttgcg cttcgccccg aggcgcccca gaaccgccag cacgccgatc tccatgcgac
     5701 ggcccatcag ttcgtcggcc gggatcgact cgcggcgcat caggtcgaag tactcggagc
     5761 ggggatcgtg ggtcgactcg atcaccttca tgacccggtc cccgttgatc tcgatctccc
     5821 ggtcctccat gtaccagccg ccgatcgaca tcacatggtt catcagatgc tcggcgtcaa
     5881 gcttcgacgg cttcttcacg aatcccaggt tgtgcagggc atcccgcagc ccctccgggt
     5941 cgttcctcac ggctgcgtcc accgcgagct gctccagttc gatctgctcc ttgtccagct
     6001 tcttggtcat cccgaagtcg aggaaggcga cccggccgtc ctccatcagc aggtagttgc
     6061 cggggtgggg atcggcgtta aaatgctgga ggtggtagat cgatccgaaa ctgccgcgaa
     6121 acaggatttc cccgaaacgg cttcgctgtt cgtggtcgag ttcgaggatc tcctcgaatc
     6181 cgatcccttc gacgaactcg gtgaccagca cccgccgacg ggaaagccgg gtgaccacgt
     6241 ccggaacgta gatgaacggg tggccccggt aggcgcgggc gaaagaacgc tggttctggg
     6301 cctcgtactc gtagtcgagt tcctccagca ccctctcgcg cagctcgcgg gtcacctccc
     6361 gggcgtcgag cccgggggcg agggcacggg ccatgcggac cagcatgccc gcgttcttca
     6421 ggtcggattc aagcgcctct gcgatgcccg ggtactggat cttgaccgct acctcccggc
     6481 cgtcgatcaa ccgcccgcgg tggacctggc caatcgaggc cgcggcgaag gcttcgtgtt
     6541 cgatcgagtc gaatagctgc tcgatcggtt ccccgtcgta ttcctcccgc aggaccttga
     6601 ccacttcctc ccacggcatc gccggcgcct ccgagcggag tttccccaac tgctcctggt
     6661 agatctcccg gtactcctcg ggaatgaagt cgatgtcgat gaaagaggcg aactgaccga
     6721 tcttcatcgc cgcccccttc atctggccca gggtcgcgac catcctcgcg gcggtttcgg
     6781 catgcctttc gtccagcttc tcctgactgg aatcaccgcc gcgaaaaag
//
LOCUS       DAOTIP010000013         6815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_13251_length_6815_cov_3.545414, whole genome shotgun sequence.
ACCESSION   DAOTIP010000013 DAOTIP010000000
VERSION     DAOTIP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13251_length_6815_cov_3.545414"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="P5138_00540"
     CDS             <1..522
                     /locus_tag="P5138_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59094.1"
                     /translation="FLSDRIGTRPLIFTGLVLQAGGLAWITAISSPTTAFGDFIVPFM
                     MAGTGMALVFPTAAATVVSSVREVETGKASGATNAIREIGGVMGVAVLSSIFAANGGF
                     TSPQAFTDGVVAAMPVAVVVLAIGAVLALLIPGKDQVSEDAGKTDRSSSDRPRGPRPV
                     GRPEPQLLAASKS"
     gene            complement(495..1358)
                     /locus_tag="P5138_00545"
     CDS             complement(495..1358)
                     /locus_tag="P5138_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRV59095.1"
                     /translation="MSAPGSFRLAVAQPVIDRREPESSRVDDAVELIRAAGEGGAELV
                     LFPESYPGPITAGLDHDAGPAVAEACRETGLAACWGRVEPTGDGRFVTVSILTDGNGR
                     EVGRYLRSHPATGDVHPVLSGAPMAPGPEPGLFELNGLRFGVVVCSELWNPELCRVLA
                     LRGAEVLLAPAGGGFHRVSDNWQLMARARAVENEMFVALTQQLFDDEFGSALIAGPEG
                     VLTEGTDPGLLITDLDLERARWLRSQDDSMVEPKQFSSLPGLLRARRPELYGELGADR
                     DGLYDFEAASN"
     gene            complement(1355..1936)
                     /locus_tag="P5138_00550"
     CDS             complement(1355..1936)
                     /locus_tag="P5138_00550"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HRV59096.1"
                     /translation="MADVSTEVPHGTAKRFDLAAGESIRIETPDGAQGGDFSFPGFDQ
                     ALTRNKLGWKRFGRPWLVFAAEPGDSLFDVDGEATFELVALVGDGVADIMYPGCWDEV
                     YEDRRPGCQDLISAALSIGRRDLTGMLSFFIGYSADDIAYRGLAGVDLNPGDHLEFRA
                     LRDVSCAVSACPDLDIPGWRPGGLVVTVKEPVQ"
     gene            2073..2375
                     /locus_tag="P5138_00555"
     CDS             2073..2375
                     /locus_tag="P5138_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59097.1"
                     /translation="MMARKRTFHVAFGLLGLMACAGMLSGIGALLMVAPFLLLFGLLL
                     AGLYPGERAIQAVALLLSARPRSRRPFTSSLDFRPVLARAIRYPGGSGSRAPPHFA"
     gene            2428..3099
                     /locus_tag="P5138_00560"
     CDS             2428..3099
                     /locus_tag="P5138_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRV59098.1"
                     /translation="MEVLRIRSLTFGRVLVPILLVAALAVIVTGCGSDDSSTELNGIT
                     RVPPAKTGNLSLPDYSPQSGGKKVAVRGNEDGLMLVYFGYTSCPDVCPTTLADLRNAL
                     AELDPAQRERVQVSMVTVDPDRDTGRVLNGYLAHFFEPGEYHSLRTTDQALLNRADEA
                     FGASHKLGKPDADGNYDVSHTALLYAVDPKGTVEVEWPFGTDADVIADDLKTLLDNNS
                     QNPDA"
     gene            3124..3684
                     /locus_tag="P5138_00565"
     CDS             3124..3684
                     /locus_tag="P5138_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRV59099.1"
                     /translation="MKKLVPLATVVLALGIFVAACGSDDSDSGSSGVEVTDVWARPTT
                     PTADAGAIYMTINSKDGDSLTAAKVPSSIAGMTQIHETTTDGEATSSEDSMESGDSMS
                     SDDSMGSDDSMDSDHSMEGGSMMGMKEVSQIDIPAGETVTLEPGGFHIMLLDLKGQIK
                     AGDTIPVTLTFEKAGTVEVDATAQDQ"
     gene            3662..4069
                     /locus_tag="P5138_00570"
     CDS             3662..4069
                     /locus_tag="P5138_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59100.1"
                     /translation="MPQPRISKSEDERGPARRRAPIVIAVAGLAVAAIVAVLLIGSGS
                     EPEGRTIELVIPKGTSARIDSGESVPGIPDRIEASVGDTLLIVNRDSATQFVSGFAIS
                     PGQEVNIPLSRPGTYSTNCSAHKDKSIEMVVKS"
     gene            4066..4389
                     /locus_tag="P5138_00575"
     CDS             4066..4389
                     /locus_tag="P5138_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRV59101.1"
                     /translation="MKKFLLLLSIVPIAIAGCGGSDEGSPSGAKTAGAELFNSAGCAG
                     CHALSEADANGPVGPDLDGLKLDEQRVQEQIENGGGSMPSFKGRLTPAEIDRLARFVS
                     RASQS"
     gene            complement(4426..5139)
                     /locus_tag="P5138_00580"
     CDS             complement(4426..5139)
                     /locus_tag="P5138_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRV59102.1"
                     /translation="MRIPRSTIFSALALAVLLLFAPAPNAAAPQPTAPAGFAHSSYRS
                     ENAFRGEILTINRKLRKRMTGNSWHRGCPVGLSDLRLLKLRYRSFPGAVRRGYLVVHE
                     TAAPRILGVFRRLFNAGYRIRRMRLVDAYGADDHRSMNADNTSAFNCREVAGRPGVWS
                     RHAFGRAIDVNPVENPYVAGSHVSPARGRPFASRTPRRRGMISQGGRVVRIFAGIGWE
                     WGGDWYGARDYQHFSASGR"
     gene            5240..5542
                     /locus_tag="P5138_00585"
     CDS             5240..5542
                     /locus_tag="P5138_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRV59103.1"
                     /translation="MRKSVAIGIAGVTAVVVAAWRQVQRDNTLETDSGPTPAATTSPA
                     GSTAGPQSSSPEALFTPSVSEKSTKAELYEMASELGIEGRSKMNKSELLEAIRTAS"
     gene            complement(5556..6176)
                     /locus_tag="P5138_00590"
     CDS             complement(5556..6176)
                     /locus_tag="P5138_00590"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRV59104.1"
                     /translation="MNELEERLTAEETFAGDAGWEVLRSGLLERADREELIDLAYERH
                     ETPFGRLLVVAGDGGIVRIALDAEREEEVLDVLASRISPRIGLVGRDSLAAARHQLDR
                     YFEGELTEFDLTLDWRLSRGFRRQVLHETAAIPYGSTASYAQMAARAGSPRAVRAAGS
                     ALATNPLPIVVPCHRVLRSDGRVGAYLGGTSMKEALLAMERDRSPV"
     gene            complement(6169..6660)
                     /locus_tag="P5138_00595"
     CDS             complement(6169..6660)
                     /locus_tag="P5138_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRV59105.1"
                     /translation="MKENESKTPGPPPFELALEDHGPRVHSWLVTRVGPDRADDVFQE
                     TMLSALKAWNRVEVGSVRAWLFAIARNRAVDDDRRFGRRPAPEGEIDSWPSAEVTERF
                     EDPVWDRVRELPDKQRTAVTLRFRGDLTHREIGAVMETSEAAARRNVFEGLKRLRERI
                     DDE"
BASE COUNT         1219 a         2299 c         2167 g         1130 t
ORIGIN      
        1 ttcctcagcg accggatcgg caccaggccg ctgatcttca cgggcctggt cctgcaggcg
       61 ggtggtctcg cctggatcac tgcgatctcc agcccgacca ccgccttcgg tgacttcatc
      121 gtccccttca tgatggccgg caccgggatg gccctggtct tcccgaccgc cgccgcgacc
      181 gtggtttcct cggtgcgtga agtcgagacc ggaaaggcgt cgggagcgac caatgcgatc
      241 cgcgagatcg gcggggtcat gggagtcgcc gtgctctctt cgatcttcgc ggccaacggc
      301 ggcttcacca gtccgcaggc gttcaccgac ggagtggtcg ccgccatgcc ggtcgctgtc
      361 gtggtgctgg cgatcggggc ggtcctggcc ctgctcatcc cgggaaagga ccaggtctcg
      421 gaggatgccg gaaagacaga ccggagcagc agcgaccggc cgaggggacc ccgccccgtc
      481 ggccggcccg aacctcagtt gctggcggcc tcgaagtcgt agagaccgtc ccgatcggcc
      541 ccgagctcgc cgtagagctc ggggcgacgg gcccggagca gccccggcag ggagctgaac
      601 tgcttcggtt cgaccatcga gtcgtcctgg ctcctgagcc agcgggcccg ttccaggtcc
      661 aggtcggtga tcagcagtcc cgggtcggtg ccttcggtca gaacgccctc ggggccggcg
      721 atcagagccg atccgaactc gtcgtcgaag agctgctggg tcagggcgac gaacatttcg
      781 ttttccaccg cccgggcccg ggccatcagc tgccagttgt cggaaacacg gtggaagccg
      841 ccgccggccg gggcaagcag tacctcggca ccccgcaggg ccagcacgcg gcagagttcg
      901 ggattccaga gttccgagca gacgaccacc ccgaacctga ggccattcag ttcgaacaga
      961 cccggctccg gaccaggggc catcggcgct cccgagagaa ccgggtgaac gtcacccgtg
     1021 gcagggtggc tgcgcaggta gcgaccgacc tccctcccgt tgccgtcggt gagaatcgag
     1081 actgtgacga accggccgtc gccggtcggt tcgacccggc cccagcaggc cgccaggcct
     1141 gtctcgcgac aggcctccgc aactgcgggc cccgcatcgt gatcgaggcc cgcggtgatc
     1201 ggtcccggat agctctcggg aaacagaacc agctccgctc cgccctcacc cgcggcgcgg
     1261 atcagctcga ccgcgtcgtc gacccgtgag ctttccggtt cccggcgatc gatcaccggc
     1321 tgggcaacgg ccagccggaa gctgccgggc gcgctcactg gaccggctcc ttcaccgtca
     1381 ccactagccc acccggccgc cagcccggaa tatccaggtc cggacaggcc gagacagcac
     1441 agctgacgtc acggagggcc cggaattcga ggtggtcgcc ggggttcagg tcgacccccg
     1501 ccaaccccct gtaagcaatg tcatcggccg aatatccgat gaagaaggaa agcatccctg
     1561 tcaggtcgcg ccgtccgatc gaaagcgctg ccgagatcag atcctggcag ccgggtctgc
     1621 ggtcctcgta gacctcgtcc caacagcccg ggtacatgat gtcggcgacg ccgtcaccca
     1681 ccagcgctac cagttcgaag gtggcctccc cgtcgacgtc gaagagcgag tcgcccggct
     1741 cggccgcgaa aaccagccag ggccggccga accgcttcca gccgagcttg tttcgggtca
     1801 gagcctgatc gaaaccggga aagctgaagt cgccgccctg cgccccgtcc ggggtctcga
     1861 tccggatcga ctcgcctgcc gcgaggtcga atctctttgc ggttccatgg ggaacctcgg
     1921 tcgatacgtc tgccattgcc atcgcacccg ctttcggtcg ggtcgcactc cttaatcgaa
     1981 gcggagctta cgaactcagt gcgacggatc cgttcgatca taacccgtgt aaaaccgaag
     2041 cttccgaccc cggcgatggt tgtaacctcc agatgatggc ccgcaagcgg acatttcatg
     2101 ttgcgttcgg cctgctcggc ctgatggcct gcgccgggat gctcagcgga atcggcgccc
     2161 tgctaatggt ggcgcccttc ctgctgctgt tcgggctgct gctggccggg ctctacccgg
     2221 gcgagcgggc gatccaggca gttgcgctgc ttctctcagc ccggccccgc agccgacgcc
     2281 cgttcacttc cagccttgat ttccgacccg ttctggcccg cgccatccgg tacccgggcg
     2341 gatcgggttc ccgcgctcca ccccacttcg cctgatccag gagaccggcc ggcctgaccg
     2401 gcaccgaagc gaagtgaaag gagcctcatg gaggttctgc gaatacgttc actcacgttc
     2461 ggacgggttc tcgttccgat cctgctcgta gccgcactgg cggtaatcgt gaccggatgc
     2521 ggaagcgacg attcgtccac cgaactgaac ggcatcaccc gggttccgcc cgcaaaaaca
     2581 ggaaatctgt cccttcccga ctacagcccg caatccggcg ggaagaaggt cgccgtgagg
     2641 gggaacgaag acggtctgat gctggtctac ttcggctaca catcctgtcc ggatgtctgc
     2701 ccgaccacgc tcgccgacct gcgtaacgcc ctggcggagc tggaccccgc acagcgtgaa
     2761 cgggttcagg tgtcgatggt cacggtagac cccgaccggg ataccggtcg tgtgctgaac
     2821 ggatacctgg ctcatttctt cgagcccggt gaataccact cgcttcgcac caccgatcag
     2881 gcgcttctga atcgcgccga tgaggctttc ggcgcaagcc acaagctcgg caagccggat
     2941 gctgacggca actacgacgt gagccacacg gctcttttgt acgcggtcga cccgaagggg
     3001 accgtcgagg tcgagtggcc gttcggaacc gatgccgacg tcatcgccga tgacctgaaa
     3061 accctgcttg acaacaacag tcagaacccg gacgcatagg cgatccggaa aggagataag
     3121 tcaatgaaaa agctagttcc acttgccacc gtggtgcttg ccctcggcat cttcgtcgct
     3181 gcctgcggct cggatgactc cgacagcgga agctcgggag tcgaagtgac cgatgtctgg
     3241 gcccggccca ccaccccgac cgcggatgcc ggggccatct acatgacgat caactcgaag
     3301 gacggagaca gcctcacggc ggccaaggtg ccttcgagca ttgccggcat gacccagatc
     3361 cacgagacca ccaccgatgg tgaagcaacg agttccgagg attccatgga atccggtgac
     3421 tcgatgagtt cggatgactc gatgggttcg gatgactcga tggactccga tcacagcatg
     3481 gaaggcgggt cgatgatggg catgaaggaa gtcagccaga tcgacattcc ggctggcgaa
     3541 accgtcaccc tcgaacccgg cggctttcac atcatgctgc tcgacctgaa gggtcagatc
     3601 aaggcgggtg acacgattcc ggtgaccctg accttcgaga aggccggcac ggtcgaggtc
     3661 gatgccacag cccaggatca gtaagagcga agacgaaagg ggtccggcca ggcgccgggc
     3721 cccgatcgtc atcgcggtcg ccggcctcgc ggtggcggcg atcgtcgccg tgctcctgat
     3781 tggaagcgga agcgagccgg aaggccggac gatcgaactc gtcatcccga agggcacctc
     3841 ggcccggatc gactccgggg agtctgttcc cggcatcccg gatcggatcg aagccagcgt
     3901 cggggacact ctcctgatcg tcaatcgcga cagcgccacc cagttcgttt cgggtttcgc
     3961 gatctcgccc ggacaggaag tgaacattcc cctctcccgg cccggtacct attcgaccaa
     4021 ctgcagcgcc cacaaagaca agagcatcga gatggtggtg aaatcgtgaa gaagttcctg
     4081 ctgttgctgt caatcgtccc gatcgccatc gccggttgcg gcggcagcga cgaggggagt
     4141 ccttccggcg cgaagaccgc cggagccgag ctgttcaact ctgccggctg cgccgggtgc
     4201 cacgcgctca gcgaagccga tgcgaacgga ccggtgggtc ccgacctgga cggtctgaag
     4261 ctggacgaac agcgggttca ggagcagata gaaaacggcg gtggctccat gccttccttc
     4321 aagggaagac tgactccggc cgaaatcgac cgcctggcca ggttcgtttc cagagccagc
     4381 cagtcctgat cgaccgggcg cgccgcaggg ccgactgcgc gggcgctacc ggccgcttgc
     4441 cgagaagtgc tggtagtccc tggccccgta ccagtcaccg ccccactccc agccgatccc
     4501 ggcgaatatc cggaccaccc ggccaccttg gctgatcatt ccgcggcggc gcggggtccg
     4561 gctggcaaag ggacgtcccc tggccggcga tacatgactg ccggccacgt aggggttctc
     4621 gacggggttc acgtctatcg cccggccaaa ggcatggcgt gaccagaccc cgggcctgcc
     4681 ggccacttcg cgacaattga aggcggacgt gttgtccgcg ttcatcgacc ggtgatcgtc
     4741 agccccgtag gcgtcaacga gccgcatgcg tcggatccgg tagccggcgt tgaagagcct
     4801 ccggaagacc ccgaggatcc gtggggctgc ggtctcgtgg acgaccaggt atccacgccg
     4861 cacggcaccg ggaaagctcc ggtaacggag cttcagcagg cgaaggtcgg acagcccgac
     4921 cgggcagcca cgatgccacg agttgccggt catccgcttc cgcaacttcc ggttgatcgt
     4981 gaggatctca cccctgaagg cgttttcgct ccggtaggaa gaatgcgcga agccggccgg
     5041 agccgtcggc tgaggtgctg cggcgttcgg ggccggggca aacaggagca acacggcgag
     5101 ggccagggcg gagaagatcg tcgagcgggg aatcctcacg atccgagact agacaatcgt
     5161 ccagaagccc aaaccgcatc gtcggtagag tctccgctgt gatcaattgc ttccctacca
     5221 gtcacaaatg gtcgaagtca tgaggaaatc cgttgccatc ggcatcgccg gggtgaccgc
     5281 agtcgtggtg gcagcgtggc ggcaggtcca gcgcgacaac accctcgaga ccgattccgg
     5341 gcccaccccg gccgcgacca cctcgcccgc cggatcgacg gcaggcccgc aatcgagctc
     5401 accggaagcg ctcttcaccc cgtcggtcag cgagaagtcg accaaggcgg agctctacga
     5461 gatggccagc gagctgggca tcgagggtcg ctcgaagatg aacaagtccg agctgctcga
     5521 agcgatccgc accgcctcct gacgccgacg gccggtcaga ccggtgaccg gtcgcgttcc
     5581 atggcaagga gcgcttcctt catcgaagtg ccgcccagat aggcaccgac cctgccgtca
     5641 gagcgcagca cccggtggca ggggaccacg atcggcaggg gattggtggc cagagcggaa
     5701 ccggccgccc tcaccgccct cgggctgccc gcccttgcgg ccatctgcgc gtagcttgcg
     5761 gtgcttccat aagggatcgc cgccgtctca tgcagcacct ggcgccggaa gccccggctc
     5821 agccgccagt cgagggtcag atcgaattcc gtcagttcac cctcgaagta ccggtcgagc
     5881 tggtgtcggg cagcggccag cgagtcacgc ccgaccaggc cgattctcgg cgagatccgc
     5941 gaggcgagca cgtccagcac ttcctcctcc cgctctgcgt caagggcgat cctgacgatt
     6001 cctccgtcac cggccacgac cagcagacgg ccaaagggag tttcgtgccg ttcgtaagcc
     6061 aggtcgatca gctcttcccg atcggcccgt tcgagcaacc cggaccggag aacctcccag
     6121 cccgcatccc cggcgaaggt ctcttcagcg gtcagtcttt cctcgagttc attcatcgtc
     6181 gatcctttct ctgagtcgct tcagcccctc gaagacgttc cggcgtgctg ccgcttcgga
     6241 ggtctccatg accgcgccga tctcccggtg ggtcagatcg ccccggaacc ggagggtgac
     6301 ggccgttcgc tgcttgtcag ggagttcccg gacccggtcc cagaccgggt cctcgaagcg
     6361 ttcggtgacc tctgcagagg gccagctgtc gatctcgccc tccggtgccg gtctccggcc
     6421 gaaccggcga tcgtcgtcga ccgcgcggtt gcgggcgatc gcgaagagcc aggcccggac
     6481 agacccgact tccacccggt tccatgcctt cagggccgag agcatcgttt cctggaagac
     6541 gtcatcggcc cggtccgggc cgaccctcgt gaccagccac gaatggacgc gcggtccgtg
     6601 atcctcgagc gccagttcga aaggtggcgg cccgggggtt ttcgattcgt tttctttcac
     6661 tgcttccggg acgagcgagg gaccggattc gtgagatcga caccgaccgg gcacatgcca
     6721 caactctatt cttggccaat gcccctgtcg gactcatacg acggcttcct gatcgacctc
     6781 gacggggtgg tctggctggg gcacgatccg ataga
//
LOCUS       DAOTIP010000014         6750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_13437_length_6750_cov_3.291561, whole genome shotgun sequence.
ACCESSION   DAOTIP010000014 DAOTIP010000000
VERSION     DAOTIP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13437_length_6750_cov_3.291561"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="P5138_00600"
     CDS             <1..745
                     /locus_tag="P5138_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59106.1"
                     /translation="GSLRPLRVAGLTVVRQLSSSWINICALKFNGRVFCWGDRAGFGS
                     KGGIRFHPTRVPRIRNAVSIAVGTYSSCAVKRSHRVVCWSNPEFRIRPKRNLGKVLSL
                     QVDLENGCAIRVTHRVVCWGTNFEGQLGVGRRPGASSKPLLVRGLGRVKALSTDTSTA
                     CAIRLSGKVLCWGANDGAVGDGTYRTRFHPVSVPGVTAARTISVNGAGACAASSYLQT
                     WCWGVNSVGEMGTGTLVDQLLPARILGVR"
     gene            775..3564
                     /locus_tag="P5138_00605"
     CDS             775..3564
                     /locus_tag="P5138_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRV59107.1"
                     /translation="MRRPAGTVTDFRRLLLVLSLAIFGVSIFAQQSSAALTARDSDPV
                     VLKGSQVGNLTGIDPDRLVAFRWTGSDWDQVPVQVDERKTINLVSLYPWVTGGSNQGY
                     VTNNLSSVNIEVYADAGTRTGADTDATLDSNDEVAFMAFDSGSKAPIESDPAHVVARS
                     GMEFRLEDPLNGDSSYVYLFKSDGTLSPGAGEQYVTYSPSLQNGIGSSYRDNYRYFFP
                     LNASPPANGYNNENTTLITPSYSVHSIDRWIDDQLKITAGSSTGADILDRDKVAFTPG
                     YCGRTEDTFTGRETFNSNDNAEGAFVTNRSGPVRAIRSYVGANSGPYTQREQIFYRDR
                     QDVRTFLRVHSIPYVMSFTDYSPDASGMTWRSSINTTGVTVDGTPDLFAVPGFTDPRD
                     LTGIQYPNAGSGVSGWEQITGAQGTVDTVSRLLTDISNVAATAYYLDNSSPSTTFNQS
                     QMTGDPTYQCTGDAYAYGASGLQLKVATPIGADLPNTDPRAGGSENNVTMERSVFYGP
                     PGGDAAEAEGRKAQVDSPIEFTSERHVLPPDGPPTVSPSGKDFGSLELSQGPGATQQF
                     TIHNDGNYGLIVGQATFTGSGGFQFDGDTCSNQTVAAGSSCSVGVRFDPDSTGLANGS
                     LSVPNQSFHPDGNTVHPDLAIALTGTGEADPLLAVSPDNFDFGPSETGVSTPSQLFTI
                     SNPAHFSRYVEQVAKTGTGAVHFQNTSGNCTARTLAPGASCTYRLAFKPTSAGAKGAI
                     LEVRGTGGTILGYALVIGTGIAGVTGPTGPSGDTGSTGETGPTGPTGPTGETGETGPT
                     GATGETGPTGPIGPTGETTVTGPTGPTGSTGETTTGPTGPTTPTGPTGPTSPTGSTGE
                     TSEPVGPTGPQGPTATCLRNRKNLAAAKRRLAKAKKAVRKTRKTKARKVARRKVTRIG
                     RDVRRISGQVRRACA"
     gene            3597..5447
                     /locus_tag="P5138_00610"
     CDS             3597..5447
                     /locus_tag="P5138_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59108.1"
                     /translation="MGDHAYRTFARIGAVLGLAAITAVGFAGSSTAAPADRGDDPVVL
                     KGSQLARLTGAAPGKVVGFKWNGKWIQVPVQIDERHTVSARTLYPADADNKYILGTTF
                     DIEVYADPKTRSGADENSNFDADDELVFMGGDSGKAAPTSAVAPAAVAPGSATRVAVD
                     DPVGGGKGYVYLFRSTGNLDPSAGKDYVDYDFKLTKLTSGQNMKDDYGYVNATNPEDS
                     TVTTDSYQLHSTGRWMEDEMKISAGGASDADILDREAVSAGSLNSCGRSEYTFSGNWD
                     LDAVIGNERPDDDDEGTYVAVIDGPVRAVRDFMGANSGPYVESIHKYYADREDKAINV
                     RVHPIPYMYVWTDYSEAAVGMTYRDQLNTGGVTVDGSPDAINSPAPGDVEDGQYFWQQ
                     LSGSQGSVTTLVSAQASVIDGDNPPYAGYYLDDVTPTGSKEKQCGGDLKAYGASGFGI
                     DGTFPNTDPNLAPIFVNTRDLSVNRVRYFGGPDETATEAEALRNRVQQPLTGTASKAS
                     VKAPTENLKLAIKGKKPKARPGKALRLKVKLSNSGNTASSAVKVCLKGGKLSRRACKT
                     VSTVGSGKAKLLTLKSKVRKGVRGKRIMVTITARSSSSALGRGVLLRVKH"
     gene            complement(5451..6386)
                     /locus_tag="P5138_00615"
     CDS             complement(5451..6386)
                     /locus_tag="P5138_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRV59109.1"
                     /translation="MFETRTDQWHEAGLGEQIVDTPRGRTWPRLLLFAILIAVVLFAF
                     NHRREVLPAGYGLEARILTAVLLFILGWGFASALGRTLTPFIMRRLDPGTAGTLSFAI
                     RLITILLVALVALGIAGVKPATLAVGGAAVTIVLGLAAQQTLGNIFAGVVLQSTRPFR
                     VGERVRLTGGPVAGSTEGTVSSLGLFYTTLLKGQDRMLIPNSLLLLLAVEPLREPEAI
                     DLRARFDSHLSPATVQDMLRQAITVPTLRPPRIELEEVDRDEVVLRISATPVTPSDGA
                     RLAEQIVSVTRGTFEYEKAPAPDQKPDPDVPLGHS"
BASE COUNT         1252 a         2254 c         2196 g         1048 t
ORIGIN      
        1 aggatcactg cgccccctgc gggtcgccgg cctgaccgtg gtgcgacagc tcagcagcag
       61 ctggataaac atctgcgcgc tcaagttcaa cggcagggtt ttctgctggg gtgaccgggc
      121 gggcttcggg tcgaagggag gaatccggtt ccatcccacc cgggtccccc ggatccggaa
      181 tgcggtgtcg atcgcggtcg gcacctattc ctcctgcgcg gtgaagcgca gccaccgggt
      241 tgtctgctgg agcaatccgg agttccggat ccggcccaag cgcaacctcg gcaaggtcct
      301 ctcgctccag gtggacctcg aaaacggatg tgccatccgg gtgactcacc gggtggtctg
      361 ctggggcacg aacttcgaag gtcagctcgg ggttggccgc aggccggggg cgagctcgaa
      421 accccttctg gtccgtgggc tcgggcgcgt gaaggcactg tcgaccgaca catcgaccgc
      481 ctgcgccatc cgactgtccg gaaaggttct gtgctggggc gcgaacgacg gagccgtcgg
      541 cgacggcacc taccgcacgc gatttcatcc cgtctccgta cccggagtga ccgcggcgcg
      601 aacgatcagc gtgaacgggg cgggcgcatg cgccgcgagc agttaccttc agacctggtg
      661 ctggggggtc aactcggtcg gcgagatggg gacgggaacc cttgtcgacc agctgctgcc
      721 ggcaaggatc ctcggtgtcc ggtagtcccc gaccgggccg ctgacggggt gccgatgcga
      781 cgcccggcgg gcacagtcac cgattttcgc aggttgcttc tggttctctc tctggccatt
      841 ttcggcgtct ccatattcgc tcagcagtcg tcggcggcac tcactgcacg cgactcggat
      901 ccggttgtcc tcaagggcag ccaggtcggg aacctgaccg ggatcgaccc cgaccggctg
      961 gtcgccttcc gctggaccgg ttccgactgg gaccaggtgc cggttcaggt cgacgaacgc
     1021 aagacgatca acctggtgag cctctacccg tgggtcaccg gaggctcgaa ccaggggtac
     1081 gtgaccaaca atctgtcgtc ggtcaacatc gaggtttatg cggacgccgg cacccggacc
     1141 ggggccgaca ccgacgccac tctcgactcg aacgacgagg tcgccttcat ggccttcgat
     1201 tcgggatcca aggcgccgat cgaatccgac cccgcccatg tggtcgccag gtcggggatg
     1261 gaattcaggc tcgaggaccc gctcaacggg gacagctcct acgtctacct gttcaagtcc
     1321 gacggcaccc tcagccctgg tgccggggag cagtacgtca cctattcccc ctcgctccag
     1381 aacgggatcg gttcgagcta ccgggacaac taccgctact tcttcccgct caacgccagc
     1441 ccgccggcca acggctacaa caacgagaac acgaccctca tcacgcccag ctattcggtt
     1501 cattcaatcg accgctggat cgacgaccag ctgaagatca ctgccggttc ctccaccggg
     1561 gcggacatcc tcgaccgcga caaggtggcc ttcaccccgg gctactgcgg ccgcaccgag
     1621 gacactttca ccggccggga aaccttcaac agcaacgaca acgcggaggg ggcgttcgtg
     1681 accaaccgca gcgggccggt ccgggcgatt cgttcctacg tcggggcaaa cagcggtccc
     1741 tacacccagc gcgaacagat cttctaccgc gaccgccagg acgtccgcac cttcctgcgc
     1801 gtccattcga tcccctacgt gatgtccttc accgactact cgccggatgc gtcgggaatg
     1861 acctggcgtt cctcgatcaa cacgaccggc gtcaccgtcg acggcacgcc cgacctcttc
     1921 gccgttcccg gtttcaccga tccccgggac ctgaccggaa tccagtatcc gaacgccggc
     1981 tcgggggtca gtggctggga gcagatcacc ggtgcccagg ggacggtcga caccgtttcc
     2041 cgtctgctca ccgacatatc gaatgtcgcg gctaccgcct actacctcga caacagcagc
     2101 ccgagcacca ccttcaacca gagccagatg accggtgatc cgacctatca atgcaccggc
     2161 gacgcctacg cgtacggagc gagcgggctc cagctcaagg ttgccacgcc gatcggcgcc
     2221 gaccttccca acaccgaccc gcgggccggc ggcagcgaga acaacgtgac catggagcgg
     2281 tcagtctttt acgggccgcc cggcggagac gcggccgaag ccgaggggcg gaaggcccag
     2341 gtcgatagcc cgatcgaatt cacatccgaa cgccatgtgc ttcccccgga tggcccgccg
     2401 accgtgtccc cgtccggcaa ggacttcgga tcactcgagt tgagtcaggg tccgggggcg
     2461 acccagcagt tcacgattca caacgacggc aactacgggc tgatcgtcgg tcaggccacc
     2521 ttcaccggca gcggcggctt ccagttcgac ggcgacacct gctcgaacca gactgttgcg
     2581 gcgggcagca gctgcagcgt cggagtccgc ttcgatcccg attcgaccgg tctggcgaac
     2641 ggctctctgt ccgttccgaa tcagtccttc cacccggacg gcaacaccgt ccacccggat
     2701 ctcgcgattg ccctcaccgg caccggggaa gccgacccgc tgttggcggt cagccccgac
     2761 aatttcgatt tcggcccgtc cgagaccggc gtctcgaccc cgagtcagct cttcacgatc
     2821 agcaatcccg cccatttttc ccgctatgtg gagcaggtag cgaagaccgg cacgggggcg
     2881 gttcatttcc agaacaccag cggcaactgc accgccagga cgcttgctcc cggggcctca
     2941 tgcacctacc gcctcgcctt caagccgaca tcggccgggg ccaaaggcgc gatcctggaa
     3001 gtccgtggga ccggcggaac gattctcggc tacgcgctgg tgatcgggac agggatcgct
     3061 ggtgtgaccg ggccgaccgg cccgagcggg gataccggat caaccgggga aaccggaccg
     3121 acgggcccga cagggcccac tggagaaacg ggggagacgg gacccaccgg cgcaaccggg
     3181 gagaccgggc cgacggggcc gatcggcccg accggtgaaa ccactgtgac cggcccgacg
     3241 ggaccaaccg gaagcaccgg tgagacgacg accggcccga ccggacccac aaccccgacg
     3301 ggacccactg gtccgacatc gccgaccgga agcacgggtg aaacatccga accggtcggt
     3361 ccgaccggac cgcaaggtcc aacggccact tgcctccgga accgaaagaa cctcgccgcg
     3421 gcgaagcgcc ggcttgcgaa ggcgaagaag gcggttcgca agacgcgaaa gacgaaggcc
     3481 aggaaggttg cccgccgcaa ggtcacgcga atcggcaggg acgtccgccg gatcagcggc
     3541 caggttcgca gggcctgcgc ctgactcccg gcccgccagc gggtacgatg gcgcccatgg
     3601 gagaccacgc gtacaggacg ttcgcgcgga tcggggctgt gctcggcctc gccgcgataa
     3661 ccgctgtcgg cttcgccgga agctcgaccg ctgcccccgc cgaccggggt gatgacccgg
     3721 tcgtgctcaa gggcagccag ctggcccggc tgaccggggc ggcccccggc aaggtcgtcg
     3781 gcttcaagtg gaacggcaag tggatccagg tgcccgtgca gatagacgaa cgccatacgg
     3841 tcagcgcccg gaccctttat ccggccgacg cggacaacaa gtacatactt ggcaccacct
     3901 tcgacatcga ggtctacgcc gaccccaaga cccggtccgg ggcggacgag aactccaatt
     3961 tcgacgccga tgacgaactc gtcttcatgg gcggcgactc gggcaaggcg gcccctacat
     4021 cggcggttgc cccggccgct gtcgcgccgg gatcggcgac gagggtcgcg gtcgatgacc
     4081 cggtcggtgg tggcaagggt tacgtgtacc tcttccgctc gaccggaaat ctcgaccctt
     4141 cggccggcaa ggattacgtc gactacgact tcaagctgac caagctgacc agcggccaga
     4201 acatgaagga cgactacggg tacgtcaatg cgaccaaccc ggaagattcc acggtcacca
     4261 ccgacagcta ccagctgcat tcgaccggcc gctggatgga ggacgagatg aagatttcgg
     4321 ccggaggggc cagcgacgcc gacatccttg accgcgaggc tgtgtcggcc ggcagcctca
     4381 acagctgcgg gcgctccgaa tacaccttct ccggcaactg ggacctggac gcggtgatcg
     4441 gaaacgaacg accggacgac gatgacgagg gaacctacgt ggcggtgatc gacgggccgg
     4501 tccgggcggt acgcgacttc atgggcgcca actccggccc ctacgtcgaa tcgatccaca
     4561 agtactacgc cgaccgggaa gacaaggcga tcaacgtccg cgtccatccg atcccctaca
     4621 tgtacgtctg gaccgactat tcggaggcgg cggtcggaat gacttatcgc gaccagctga
     4681 acacgggcgg cgtgactgtt gacggcagcc ccgacgcgat caactcgccg gcacccgggg
     4741 acgttgagga cggccagtac ttctggcagc agctgagcgg atcccagggt tcggtgacca
     4801 cccttgtctc tgcacaggcg tcggtgatcg acggtgacaa tcctccctat gccggctact
     4861 acctggatga cgtaaccccg accggctcga aggagaagca gtgcggtggt gacctgaagg
     4921 cctacggggc cagcggattc gggatcgacg gcactttccc caacaccgac ccgaacctgg
     4981 caccgatctt cgtgaacacc cgcgacctat ctgtcaaccg ggtccgctac ttcggcggcc
     5041 ccgatgaaac ggcaaccgaa gcggaagccc tccgcaaccg cgtccagcag ccgctgaccg
     5101 gaaccgccag caaggcatcg gtcaaggccc cgaccgagaa cctcaagctc gcaatcaagg
     5161 gcaagaagcc aaaggcccgt cccggcaagg ccctgcggct caaggtgaag ctttcgaaca
     5221 gcggcaatac ggcctcctct gcggtcaagg tctgcctgaa ggggggcaag ttgtcccgca
     5281 gggcctgcaa gaccgtgtca actgtcgggt ccggcaaggc gaagcttctc accctgaagt
     5341 cgaaggtccg caagggcgta cggggcaagc ggatcatggt cacgatcacc gcccgctcga
     5401 gcagcagcgc gctcggcagg ggcgtgctgc tcagggtgaa gcactaaccc tcaggagtgg
     5461 ccgaggggta cgtcggggtc gggcttctgg tccggcgccg gcgccttctc gtattcgaag
     5521 gtgccgcggg tgaccgagac gatctgctcg gccagccttg caccgtccga aggggtgacc
     5581 ggggtggcgc tgatgcgcag caccacctcg tcccggtcga cctcctcgag ctcgatccgg
     5641 ggcggacgaa gggttggcac ggtgatcgcc tggcggagca tgtcctgcac ggtggcgggg
     5701 ctgagatggc tgtcgaaacg ggcccggagg tcgatcgcct ccggttcgcg aagcggctcg
     5761 acggccagca ggaggaggag gctgttgggg atcaacatgc ggtcctgacc cttgagcagg
     5821 gtggtgtaga agaggccgag tgaactcacg gtcccctcgg tggaaccggc gaccggcccg
     5881 ccggtgaggc gcaccctctc gccgacccgg aatggtcggg tgctctgcag gaccacgccg
     5941 gcgaagatgt tgccgagcgt ctgctgggcg gcgagaccga gcacgatggt gaccgcggcg
     6001 ccgccgaccg ccagggtggc cggcttcact ccggctatcc ccagggcgac cagtgcaacc
     6061 agcaggattg tgatcaggcg gattgcgaaa ctcagggttc cggccgtgcc cgggtcgagc
     6121 cggcgcatga tgaagggggt aagggtgcgg cccagggcag aggcgaatcc ccaaccgagg
     6181 atgaagagca ggaccgcggt gaggatcctt gcttccagtc catagccggc cggcaggact
     6241 tcgcgccggt ggttgaaggc gaagaggact acggcgatca ggatcgcgaa tagaagcagc
     6301 cgtggccagg tccttcccct gggggtatcg acgatctgct ccccgagccc ggcctcatgc
     6361 cactggtcgg tccgggtttc gaacatccac cgcggcatct gggaccgggc gcggctcgct
     6421 cgccgttcct tgcgttcggc ccttttctct ttacgtttgg aagtcaaggg ggatcacctt
     6481 acaaatcccc gtttcaagag cggttaagcg gaacgggcgc ggcaacttcc acccgggaaa
     6541 gttgccgcgc ccgttgaggg tcatgctctg ccgcagctca gggccagccg tgccagccgg
     6601 cggcgtaaca gggatcgatc ccgcagggat tggtcacgac tccgtcggtc ttgatgaagt
     6661 ccgcgatctg ctggcggatc accggtgagc ttcgaatcac cgtgtcatgc ggatcgatgc
     6721 cgctggtcgt gccgagcggc gtgttggatt
//
LOCUS       DAOTIP010000015         6618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_13765_length_6618_cov_3.184367, whole genome shotgun sequence.
ACCESSION   DAOTIP010000015 DAOTIP010000000
VERSION     DAOTIP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13765_length_6618_cov_3.184367"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="P5138_00620"
     CDS             <1..409
                     /locus_tag="P5138_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV59110.1"
                     /translation="PAAVITGLTYDALPWMALPIYVALEKIDRDLVEAAGDLYASGYQ
                     QFRRVILPLSAPGIYAGILLVGIANIGDYLSAQILGGPSTTMIGNIIQTQYVQNGNYP
                     VASALSLMLMLALLLCVFVYARVFGTRTIQEYA"
     gene            423..1271
                     /locus_tag="P5138_00625"
     CDS             423..1271
                     /locus_tag="P5138_00625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV59111.1"
                     /translation="MAGKGVSSDSARTVRSRPWTRFVLPSYVFVVMAYTLVPIAVMIL
                     YTFNKAPNQRLTFSWHGFTTEWYSKLFEVQDLTQALWHSLEVAVLSSIIATLLGTPAA
                     IALARRKFRGRGGIDLVVVTNLTAPTVVVGASLLGFFIALSVPRGLMTILIAHVAFNI
                     AIVIIVVQARVSGFDGSLEEAAADLGAPPWTAFFKVTLPLIMPGIFSAFLLSFALSID
                     DYIITSFVAGQTLTFPLWVAGAVKVGIPPQVFVMGTLIFISGVLIAIASLVLQRWGDH
                     KNQVRA"
     gene            1300..1638
                     /locus_tag="P5138_00630"
     CDS             1300..1638
                     /locus_tag="P5138_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59112.1"
                     /translation="MSKRQGDRCDWCGVELTEDMGYRLLWPDKSLGTAFCRLEHVVPF
                     LMQKDQWHIWKGVEVPRDAPPVSSANGNELGENALYLVHHRGEHRIPDSFEGKEDLLE
                     WARAGGHFAP"
     gene            complement(1640..2863)
                     /locus_tag="P5138_00635"
     CDS             complement(1640..2863)
                     /locus_tag="P5138_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV59113.1"
                     /translation="MARASTGKGRGVGSRIGWPLKIFVGILLVLLVLLVLNAFALNNE
                     TKSADLNVPGAKLVNTTSGPLQVLDTGTPAGQRLREISTARKVRKSGVVGSFSATTNL
                     LDSRIPIVLIHGSAGAINWWDDLIPLLKPDHRVIAIDLLGYGGSEKPDSDYSMETQGN
                     LVSQVLTRLGVKDAVLVGHSLGGSVVTSVAEQSPELVSGLVLIDSGPDRSYGGLSGAA
                     KASQTPILGQALWRLAPDFMVRKNLSQGFAPGYDVPDKYVQDVREMTYPAYKKSYEGV
                     EDYTDQKSLPDRLSQVGKPLLVIFGEEDQIYDAREALSAYAAIPGAMTHLIPKSGHSP
                     QIEAPEQTARAINNFTWTVARTKANQQATKALQAKKRAAAAKERAAARAKKQAARAKA
                     KKSRQKSGKAKQQAQ"
     gene            2955..4229
                     /gene="murA"
                     /locus_tag="P5138_00640"
     CDS             2955..4229
                     /gene="murA"
                     /locus_tag="P5138_00640"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRV59114.1"
                     /translation="MQKFVIQGGVPLSGEITAAGNKNAALPILAACLLTEEVVTIANV
                     PRIRDTEAQISLLAALGVTVEWVEDNTVQLCAKDVEDTTLDEDLSAAIRASFLLAGPL
                     LARFGTVNMPPPGGDFIGRRRLDAFRELGARVGGERWIELSAPPGGLRAAEIFMDEPS
                     VMGTENALMAAALTPGETTILNAASEPHVQDLARLLVKMGAPISGIGSNVMIIQGQEK
                     LGGAEHKVTPDHIEIASFMALAAATRGELRIREIEPFDLVMIRRQFRRLGFQSVVEGT
                     DVIVPPAQNLRVQPDAGGAIPKIEDGPWPAFPADLTSIALALATQAEGEILIFEKMFE
                     NRLFFVDKLVAMGARITLCDPHRAIVSGPTRLHGERVDSPDIRAGMAMLIAALAAEGT
                     SQIGNIRQIDRGYENIDDRLRSLGAHIERVDE"
     gene            4239..5219
                     /gene="hisZ"
                     /locus_tag="P5138_00645"
     CDS             4239..5219
                     /gene="hisZ"
                     /locus_tag="P5138_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRV59115.1"
                     /translation="MIRPIPPGTRDVLPDEMRELRAIQSALLGTFDAAGFSEVRTPTL
                     EYADALELADRSSADSAYRFFDERGEMLALRTDMTIPIARLVADRFRDVDPPHRLSYF
                     ANAYRAVTPQRGELREFGQAGVELIGVPGPEGTAEVLELLDRSLTAVGLSESVIGLGE
                     DGLWTSLLQDAGAGPETIEQSSELLVSHDLVGVERTLTESDELSPEAITEATRMIQLR
                     GGPEVLAEAREFGGEGFAAALDSLTANYESISGRSIGRKVLIDLGMLREIGYYSGSTI
                     EVYDEAVGDVIGGGGRYDDLMNRFGLDLPAAGFTLYIERIHKAQLERGNV"
     gene            5212..5892
                     /gene="hisG"
                     /locus_tag="P5138_00650"
     CDS             5212..5892
                     /gene="hisG"
                     /locus_tag="P5138_00650"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRV59116.1"
                     /translation="MFEELRKPGGKLKMAVPRGALLGEVLDLLDRIGIETAEARGDSR
                     SLVFDGGELMLITMRPSDVPTYVSAGAADVGITGKDVLLEQSDRPVYELVDLGIGPCR
                     MVLATQKGDVAPSEHQRRLGMMKIATKYKRIAEQYFAEQGRQVELVEVKGSIELAPLV
                     GLADGIVDLVATGRTLAENNLEVREEILGSTGRLVANRVSYRIRREEVDDLAARLRAA
                     VSDAPGES"
     gene            5889..>6618
                     /locus_tag="P5138_00655"
     CDS             5889..>6618
                     /locus_tag="P5138_00655"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRV59117.1"
                     /translation="MKSRRFEWAAPGRLVGEIREFLSHEDRTVDVSGIAAEVAEKGDA
                     ALLDMTAKFDAPDAEGLSLRVGQDDALEALEALDPEVRQALEVSVANVKAVASAQIGP
                     GEKTVTLPEGHTVTVGEVPVGAAGIYAPGGRASYPSTVVMGCVTARVAGVDRVVLVAP
                     PRPDGLIDPTTLAAAALCGVDEIYAVGGAQAIFALARGTETIRPVDVIAGPGNAWVQA
                     AKREVFGEVAIDSLAGPSDLTVVID"
BASE COUNT         1095 a         2178 c         2182 g         1163 t
ORIGIN      
        1 cccggccgcg gtgatcaccg gcctaaccta tgacgccctg ccctggatgg cactgccgat
       61 ctacgtcgcc ctggagaaaa tcgacaggga cctggtcgag gcggccgggg acctgtatgc
      121 ctccggctac cagcagttcc gccgggtcat actgccgctc tcggcccccg gtatctacgc
      181 gggcattttg ctggtcggga tcgccaacat cggcgattac ctgagcgccc agatccttgg
      241 tggccccagc acgacgatga tcggcaacat catccagacc cagtacgtcc agaacgggaa
      301 ctacccagtc gcctcggccc tgtcgctgat gctgatgctg gccttgctgc tctgtgtttt
      361 cgtctatgcc cgggtcttcg gaacccggac gattcaggag tacgcatgag gaggcgagcc
      421 tgatggccgg caagggagtc tcctcggaca gcgcccgcac ggtccgctcg cggccgtgga
      481 ccagattcgt cctgccgtcc tacgtcttcg tggtcatggc ctacacgctg gtgccgatcg
      541 cggtgatgat cctctacacc ttcaacaagg ccccgaacca gcgcctcacc ttctcctggc
      601 atggcttcac caccgagtgg tactcgaagc tcttcgaggt ccaggacctg acccaggccc
      661 tctggcattc cctcgaggtg gcggtgctca gttcgatcat cgccaccctc ctcggcaccc
      721 ctgcggcgat cgccctcgcc cggcgcaagt tccgcggccg cggcgggatc gatctggtgg
      781 tggtcaccaa cctgaccgcg ccgaccgttg tggtcggagc ctccctgctc ggcttcttca
      841 ttgccctgtc ggtcccgcgc gggctgatga ccatcctgat cgcccacgtc gccttcaaca
      901 tcgcgatcgt gatcatcgtc gttcaggcga gggtgagcgg attcgacgga tcactggagg
      961 aggccgctgc cgatctgggg gcgccgccat ggaccgcctt cttcaaggtg accctgcccc
     1021 tgatcatgcc gggcatcttc tccgccttcc tgctttcctt cgccctttcg atcgacgact
     1081 acatcatcac cagcttcgtg gccggccaga ccctgacctt ccccttgtgg gtggccgggg
     1141 ccgtgaaggt cggaatcccg ccgcaggtct ttgtgatggg aacgctgatc ttcatcagtg
     1201 gtgtgctgat cgcgatcgcc agtctcgtcc tccagcgctg gggtgatcac aagaaccagg
     1261 taagggcttg accgggcttc cggaaggccg gccgcctcga tgagcaagcg gcagggtgac
     1321 cgctgcgact ggtgcggggt ggagttgacg gaggacatgg gctaccggct gctgtggccg
     1381 gacaagagcc tcggcaccgc cttctgccgc ctcgagcacg tcgtcccctt cctgatgcag
     1441 aaggaccagt ggcatatctg gaagggggtg gaggtcccgc gggatgcccc gccggtcagt
     1501 tccgccaacg gcaacgaact cggcgagaac gccctctacc tggtgcacca ccgtggcgag
     1561 caccggatcc cggacagctt cgagggaaag gaagaccttc tggaatgggc aagggccgga
     1621 ggccacttcg ccccctgacc tactgggcct gctgcttcgc ctttccggac ttctgacgcg
     1681 acttcttcgc cttcgcccgg gcagcctgct ttttcgccct ggcggcggcc cgctccttcg
     1741 ccgctgcggc tcgcttcttc gcctgcaagg ctttggtggc ctgttgattg gctttggtcc
     1801 tcgcgaccgt ccacgtgaag ttgttgatgg ctctggcggt ctgttccggt gcctcgatct
     1861 ggggcgaatg gcctgacttc gggatcagat gggtcattgc tcccggaatg gctgcgtagg
     1921 cgctgagggc ctcccgggcg tcgtaaatct ggtcctcctc gccgaagatg accagcagcg
     1981 gcttgccgac ctggctcagc cggtccggca gcgacttctg gtcggtgtag tcctcgactc
     2041 cctcgtagga cttcttgtag gcggggtagg tcatttcgcg gacgtcctgg acgtacttgt
     2101 cgggcacgtc gtatccgggg gcgaagccct gcgaaaggtt cttgcgaacc atgaagtcgg
     2161 gtgcgaggcg ccagagagcc tggccgagaa tcggtgtctg ggaggccttg gcggcccccg
     2221 acagcccgcc ataggagcgg tccgggccgc tgtcgatcag gaccaggccc gagaccagtt
     2281 ccggtgactg ttcggcgacc gaggtgacca cactgccgcc gagcgaatgg cccaccagca
     2341 ccgcgtcctt gaccccgagc cgggtcagaa cctgactgac gaggttgccc tgggtctcca
     2401 tcgagtagtc ggaatccggc ttctccgagc cgccgtagcc gagcaggtcg atcgcgatca
     2461 cccggtgatc cggcttcagc agcggaatca ggtcgtccca ccagttgatc gccccggccg
     2521 agccgtggat caggacgatg ggaatccgcg aatcaagcag gttggtggtc gcgctgaacg
     2581 agcccacgac acccgatttc cggaccttgc gagcggttga gatctcacgg agtcgctggc
     2641 cggcgggagt gcccgtatcc agcacctgga gtggacccga ggtggtgttg accagcttcg
     2701 ctccggggac gttcagatcg gccgatttgg tctcgttgtt gagcgcgaag gcgttgagaa
     2761 cgagcagcac cagcaacacc agcaggatgc ccacgaagat cttcagcggc cagccgatcc
     2821 gggagccgac gccccggcct ttgcctgtgg aagctcgtgc catgaccggt gttaccctag
     2881 acccgcttcg gtcgggctgc aatgtccccg tccggtcaca cgaaaaccgt caaccgcggg
     2941 gtacagacta agaaatgcag aagttcgtaa tacagggcgg agtgccgctc tcgggcgaga
     3001 taacggccgc aggcaacaag aacgctgcgc tgccgatcct tgccgcatgc cttctcaccg
     3061 aggaggtcgt gaccattgcg aatgttccgc ggatccgcga cacggaagcg cagatctccc
     3121 tgctcgcggc gctcggagtg accgtcgagt gggtggagga caacacagtc cagctctgcg
     3181 ccaaggatgt cgaggacacg accctcgacg aggacctttc ggccgcgatc agggcttcgt
     3241 tcctgctggc cggcccgctg ctggcccgct tcggcacggt caacatgccg cctcccggcg
     3301 gtgacttcat cggtcgccgt cgcctcgacg cctttcgcga gctcggcgcc cgggtcgggg
     3361 gcgagcggtg gatcgagctg agcgcacctc ccgggggact gcgggcagcc gaaatcttca
     3421 tggacgagcc gtccgtaatg ggaaccgaga atgccctgat ggcggcggcg ctcacgcccg
     3481 gcgagacgac catcctcaac gcggcttccg agccccacgt ccaggacctg gcccgcctgc
     3541 tggtcaagat gggcgccccg atctccggga tcggctccaa cgtgatgatc atccagggcc
     3601 aggagaagct cggtggtgca gagcacaagg tcacgcccga tcacatcgag atcgccagtt
     3661 tcatggccct ggccgccgct accaggggcg agctccggat ccgcgagatc gagcccttcg
     3721 acctggtcat gatccgccgc cagttccgcc gtctcggctt ccagtcggtg gtcgagggaa
     3781 ccgacgtcat cgtgcctccg gcccagaacc tccgggttca gcccgacgcc ggaggcgcca
     3841 tccccaagat cgaggacggc ccctggcccg ccttcccggc cgacctgacc tcgatcgccc
     3901 tggccctggc aacccaggcc gaaggcgaga tcctgatctt cgagaagatg ttcgagaacc
     3961 gcctcttctt cgtcgacaag ctggtcgcga tgggggcgag gatcaccctc tgtgacccgc
     4021 accgggccat tgtcagcgga cccacccgtc tacatggcga gcgggtcgac agccccgaca
     4081 tccgggccgg tatggcgatg ctgatcgccg cgctggccgc agaaggcacc tcccagatcg
     4141 gaaacatccg ccagatcgac cgcggctacg agaacatcga cgaccggctg cggtcgctcg
     4201 gcgcacatat agagcgtgta gacgagtagg gggatcagat gatccgtccg attcccccag
     4261 gaacccggga tgttctcccg gacgagatgc gcgaactccg cgccatccag tcggccctgc
     4321 tcggaacctt cgacgcggcc gggttcagcg aggtgcgtac accgacgctg gaatacgccg
     4381 acgcactgga gctggccgat cgcagttcgg ctgactccgc ctaccgcttt ttcgacgagc
     4441 ggggagaaat gctggccctg cgcaccgaca tgacgatccc gatcgcccgg ctggtcgccg
     4501 accgcttccg ggatgtcgac ccgccgcatc ggctcagcta ctttgcgaac gcctatcggg
     4561 ccgtgacccc ccagcggggc gagctccgcg aattcgggca ggccggggtg gagctgatcg
     4621 gagtgcccgg tccggaagga acggctgagg ttctggaact cctggaccgt tcactcactg
     4681 ccgtcggcct gagcgagtcg gtcattggcc tcggtgagga cggactctgg acgtcactgc
     4741 tccaggacgc cggggccggt ccggagacga tcgagcagag ctcggagctg ctggtttctc
     4801 acgacctggt cggagtcgaa cggaccctga ccgagtccga cgagctgagt ccggaggcga
     4861 tcaccgaagc gacccggatg atccagctgc gcgggggacc ggaagtgctg gcagaggccc
     4921 gggaattcgg tggcgaagga ttcgctgctg ccctggacag ccttactgcc aactacgagt
     4981 cgatttccgg ccgatcgatc ggccgcaagg tgctgattga cctcggcatg ctgcgcgaga
     5041 tcggttacta cagcgggtcc accatcgagg tctacgacga ggcggtcggt gatgtgatcg
     5101 gcggaggcgg ccgctatgac gacctgatga accgcttcgg cctcgacctg ccggcggccg
     5161 gtttcaccct ctacatcgag cggatccaca aggcccagct ggagcgcggc aatgtttgag
     5221 gagctgagaa aacccggcgg caagctgaag atggcggtgc cccgcggggc cctgctgggc
     5281 gaagttctcg acctgctcga ccggatcggg atcgagaccg cggaggcccg gggcgattcc
     5341 cgctcgctgg tctttgacgg gggcgagctg atgctgatca ccatgcgccc ctcggatgtc
     5401 ccgacctatg tttcggccgg cgcggccgat gtgggcatca ccggcaagga cgtcctgctc
     5461 gaacagagcg accggccggt ctacgaactc gttgatctcg gaatcggccc ctgccgcatg
     5521 gtcctggcga cccagaaggg agatgttgcg ccctccgagc atcagcgccg gctcggcatg
     5581 atgaagatcg caaccaagta caagcggatc gccgagcagt acttcgccga gcagggaagg
     5641 caggtcgagc tggtcgaggt gaaggggtcg atcgagctgg ccccgctggt cggactggcc
     5701 gacggcatcg tcgatctggt cgctaccggc cggaccctgg cggaaaacaa cctcgaggtg
     5761 cgcgaggaaa tcctcggcag caccggacgc ctggtcgcca accgggtctc ctaccggatc
     5821 cgccgtgagg aagtcgatga tctcgcggct cgactccgtg ccgcggtgag cgacgctccg
     5881 ggagagtcgt gaaatcccgc cgcttcgagt gggcggcgcc cggccggctg gtcggggaaa
     5941 tccgtgagtt tctgagccat gaggacagga ccgttgacgt aagcggcatc gcggccgaag
     6001 tcgccgagaa gggcgacgcg gcattactcg acatgaccgc gaagttcgat gctcccgatg
     6061 ccgagggact gagtctcagg gtcggtcagg atgatgccct cgaggcgctc gaagccctcg
     6121 accccgaagt ccggcaggcc ctggaggttt ccgttgccaa cgtcaaggcg gtcgcctcgg
     6181 cccagatcgg gccgggggag aagacagtga ccttgccgga gggccacacg gtcacggtcg
     6241 gcgaggtgcc ggtcggggcg gccggaatct acgcaccggg cggccgggcc tcttacccct
     6301 cgaccgtggt gatgggctgc gttaccgccc gggtcgcggg ggtcgaccgg gtggtgctgg
     6361 tcgcaccgcc aaggccggac gggctgatcg atccgaccac gcttgccgcc gccgctctct
     6421 gcggggtcga cgaaatctac gcggtcggcg gcgcccaggc catattcgcc ctggcgcgcg
     6481 gcaccgagac gatccgcccg gtcgacgtga tcgccggccc cggcaacgcc tgggtgcagg
     6541 cagcaaaacg cgaggtcttc ggcgaggttg cgatcgactc gcttgccgga ccgtccgacc
     6601 tcaccgtggt catcgatt
//
LOCUS       DAOTIP010000016         6603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_13799_length_6603_cov_3.436316, whole genome shotgun sequence.
ACCESSION   DAOTIP010000016 DAOTIP010000000
VERSION     DAOTIP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_6603_cov_3.436316"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..808
                     /locus_tag="P5138_00660"
     CDS             95..808
                     /locus_tag="P5138_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRV59118.1"
                     /translation="MSTIPQLPSTFDQEIIRCGEPEIKAELSDPERLEKIREEFEDGF
                     RIMEPVGPAVCVFGSARTRPGNPEYEQARRVGAGIARQGYAVITGGGPGSMEAANRGA
                     KDEEGLSVGLNIELPHEQGANPYVDIDKEFHYFFARKLMFVRYSWSFVIFPGGFGTLD
                     ETFEVLTLIQTNKARPHPIVLAGADFWQDLIDWIARRLAGEGRIAPSDMDLFTVCDED
                     DEIVRKAVKGFPILPYGLA"
     gene            866..1201
                     /locus_tag="P5138_00665"
     CDS             866..1201
                     /locus_tag="P5138_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV59119.1"
                     /translation="MALPTPLPPELVELITARFKLLSEPTRIRILDQLREGEHSVGGL
                     AEALDTSQQNVSKHLQALYAGGAVNRRKEGNTVYYGISDESLMAMCEAVCDGVEQRIS
                     ELSQIVARV"
     gene            1336..1581
                     /locus_tag="P5138_00670"
     CDS             1336..1581
                     /locus_tag="P5138_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRV59120.1"
                     /translation="MTASRETDKPTAWYLERVLFAMAGSVSLIGGLLALLVSPWFALL
                     PAFAGINQWLYVIFGACPASLILRRVTSLKPAAEMNR"
     gene            1578..3680
                     /locus_tag="P5138_00675"
     CDS             1578..3680
                     /locus_tag="P5138_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV59121.1"
                     /translation="MTAYGPIGRLGRWTTENFRIVAAIWLVIAVGLGFFAPKAEHALS
                     GAGWEATGSESVDVRKLVDDQFGGMSSSALMVVVHSDDSTFGSAGFDKTVNQAAAVLK
                     DSNDVKGVVLPSKQSWVSADGHTAVIQAGAASDSNQMVHAADDLKEELAGLETSGTTV
                     SLTGSSGMWSDFNQANKEEMMKSEVISWPVTLLILVLAFGSLVAAGLPLMLTILGLMA
                     AAGSLYLGTQLFDISIWAMNFAMMFALALGIDYALFIVYRFRGALTSNDSTSDAIAET
                     MDTAGKAVFFSGVTVLISLSAVLLVPSPAFRSMAIGVMLAVAFVLAASLTLLPGVLGK
                     LGHRVNKLSLPWVHTGEHRSPKFAAWGERIWKRPLLFGVPALLILLALCVPVFSLDTG
                     MPSIKVVPEGDGARVGYEQVADSFGAGAPGTLQVVAPNSEVAQATTALKSDKGIAGVM
                     VAGQSPQGYTLIAATPTVDPSDPAMGPTIDRVRDELPAGALVGGAAAENHDLENALGD
                     TTAPAIAIVMTLGFLLLLIALRAPILAAIAVLTNLLAVGAAFGVAQLIFQEGHLTGLL
                     GFESQGFLDAWAPVFFFAMVFAISMDYSVFLLSSAREHWEKSGDAKEALIGGLAHSGR
                     VVFAAAGVMVAVFFTFALSGPLPPREMGIILGVAVLLDAALVRLLLLPVLMRLAGTKL
                     WWIPTWIDRRLPKVSFGH"
     gene            3729..5324
                     /locus_tag="P5138_00680"
     CDS             3729..5324
                     /locus_tag="P5138_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59122.1"
                     /translation="MTRLSPLFVALFALVLVTTPARAVEFPEGVEAGALTDAPLLASN
                     CDRIKEDGVKTTVIGAVMTRIVKSPGTYDFSTLDGQVNAAIGCGIVPAIRVFIQPSSS
                     DDRGMPDDLDAYSAFFGALVGHMKGRVTRFGVENEVIAPTHWTDTPAEYFQLLKIAAD
                     AAHAANPDAILLDSVSSSGGFSIIEARKQWLAGDLDGALATIQESQTNDLGGGPYLTS
                     TSQVPAYLSSAKVLKMQEFYDQLVAHKNLIDALQLHYYGPASSIPSTIAMVRNDGFNM
                     PIEIWELGHRYLDGRPFEQQDHADESTRLIATAVGEGARYVVLQQYFDKLANKMYGLI
                     DGDGNSRQARFAVRNTLSMLRDTTSARRLRAGSGTDGYRFDRPGGWRRVLWATGAAVR
                     PGKALGIRSTTVAVTGNQGGRSHERISSVTVGKSPRWFEPDMLEIWRQKARRKGFLRV
                     KAHCPAASPTRFCRGWVKFRGGKGKRKVVAARKYKIGRGASRVIDVRRRKQRKRVRPQ
                     VIFATPFSCPGKLNHCRSWQNLR"
     gene            complement(5341..5790)
                     /locus_tag="P5138_00685"
     CDS             complement(5341..5790)
                     /locus_tag="P5138_00685"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRV59123.1"
                     /translation="MRILFVCLGNICRSPSAEAVMRGLIANQEIEGVEVDSAGTGDWH
                     VGESADPRAISAAIERGVEISGSARQVRSSDFEEFDLILAMDRSNHANLLAMEGADPG
                     KVRLFREMAGEGEVDVPDPYFGGEDGFDEVLDILERGCVVVLEEYAN"
     gene            complement(5799..6293)
                     /locus_tag="P5138_00690"
     CDS             complement(5799..6293)
                     /locus_tag="P5138_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59124.1"
                     /translation="MTGRTRATLAIVIGSVGILLGLLGTITAFNAKNAVDSDTNTTSQ
                     IQTLVQDKFQQAQSKQDQLEANQKSEAEKLVSQLTSGEKNLLQKINGNHKQIKRAIRQ
                     NRKLRNQLNALSSRQRELTDELAGVETRQDNDYTELSQRINRLNKNLRSLTNRVNRLS
                     GYAN"
     gene            complement(6331..6555)
                     /gene="secG"
                     /locus_tag="P5138_00695"
     CDS             complement(6331..6555)
                     /gene="secG"
                     /locus_tag="P5138_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRV59125.1"
                     /translation="MYTIMAIIQVLLSVLLIFLVLLHSGKDAGLSGAFGIGGGGSSIG
                     GGSMVEQNLDRATVVTAILFTLNTILLIKL"
BASE COUNT         1132 a         2143 c         2108 g         1220 t
ORIGIN      
        1 gccccggtcg tcgatcacgc cgaggagagg cgtttcgcga tcgagcgcta ccgcgccgcc
       61 gccgatgccg ggtcccggga ataggctgcc ggtgatgagc acgatcccgc agctgccaag
      121 caccttcgac caggaaatca ttcgctgcgg cgagccggag atcaaagccg agctatccga
      181 cccggaacgc ctcgagaaga tccgggagga gttcgaggac ggtttccgga tcatggagcc
      241 ggtcggtccg gccgtctgtg tgttcggctc ggcgaggacc cggcccggca accccgagta
      301 cgagcaggca cggagggtcg gggccggaat cgccaggcag ggttatgccg tgatcaccgg
      361 cggcggaccg gggtcgatgg aagccgcgaa ccgtggagcc aaggacgaag aaggcctttc
      421 ggtcgggctc aacatcgagt tgccgcatga gcagggggcg aatccctacg tggacatcga
      481 caaggagttc cactacttct tcgcccgcaa gctgatgttc gtccgctact catggtcgtt
      541 cgtgatcttc cccggcggct ttggcaccct ggacgagacc tttgaggtcc tgaccctgat
      601 ccagaccaac aaggcccggc cccaccccat cgttctggcc ggcgccgact tctggcagga
      661 cctgatcgac tggatcgccc ggcgcctggc cggcgaagga aggatcgccc cctccgacat
      721 ggacctgttc acggtctgcg acgaagacga cgagatcgtc agaaaggccg tgaagggttt
      781 cccgatcctt ccttacggcc tcgcctgagc ggagagaagt tcacaactcg gcgggcaaat
      841 ggttgtcgag ttgtatgctt gggacatggc actcccgacc cccctcccac ccgaactggt
      901 cgaactgatc accgccaggt tcaaactgct ttcggaaccg acgcggatcc gcatcctgga
      961 tcagctacgc gagggagaac acagcgtcgg tggactggcc gaagccctcg acaccagcca
     1021 gcagaacgtc tccaagcatc tccaggcgct ttacgcaggc ggagcggtga atcgccgcaa
     1081 ggagggcaac acggtctact acgggatttc cgatgaaagc ctgatggcga tgtgcgaagc
     1141 ggtctgcgac ggcgtcgagc agcggatctc cgaactaagc cagatcgtag cccgggtcta
     1201 ggggacccgc cgccggcaga aagagctatg gccggcaaga cccgatcccg gggttgccag
     1261 ccgcctcgca agctgctaat ctattccact atacagttgt gaacttgtga acctttgact
     1321 tcgaatggag taaaaatgac tgcgagtcgt gagacggata agccgacggc ctggtacctg
     1381 gaaagggtgc tgtttgcaat ggccggatcg gtgagcctga tcgggggtct actcgccctg
     1441 ctggtttccc cctggtttgc ccttctgccg gccttcgccg gcatcaatca gtggttgtac
     1501 gtgatcttcg gcgcctgccc ggcgtcgctc atcctccgtc gcgtcacctc actcaagcct
     1561 gccgcggaga tgaaccgatg accgcctacg gaccgatcgg ccgcctgggc cgctggacga
     1621 ccgagaactt ccggattgtc gcggccatct ggctggtcat cgcggtcgga ctcggcttct
     1681 ttgccccgaa ggccgaacat gcgctttccg gcgccggctg ggaggcgaca ggctccgagt
     1741 cggtcgacgt cagaaagctg gtcgacgatc agttcggcgg catgtcctcc tcggcgctta
     1801 tggtcgtcgt ccacagcgac gactcgacct tcggcagtgc gggattcgac aagaccgtca
     1861 accaggcggc cgccgtgctc aaggattcca atgacgtcaa gggcgtggtc ctcccctcga
     1921 agcagagctg ggtttcggct gacggccaca cggctgtcat tcaggccggc gcggcctccg
     1981 attcgaacca gatggttcac gcggccgatg acctcaagga ggaactcgcc ggtctggaga
     2041 cctccgggac tacggtttcg ctgaccggtt cgtcgggcat gtggtccgac ttcaaccagg
     2101 ccaacaagga agagatgatg aagtcggagg tcatctcctg gcccgtcacg ctgctgatcc
     2161 tggtcctggc cttcggctct ctggtcgcgg ccggtctgcc cctgatgctg accattctcg
     2221 gcctgatggc cgcggccggt tcgctctacc tgggcaccca gctgttcgac atctcgatct
     2281 gggcgatgaa cttcgcgatg atgttcgcgc tcgcgctggg catcgactac gcactattca
     2341 tcgtgtaccg attccgcggt gcattgacct cgaatgactc gaccagcgat gccatcgccg
     2401 aaacgatgga cacggccggc aaggcggtgt tcttctccgg cgtgaccgtg ctgatctccc
     2461 tcagcgcagt gctgctggtg ccgagcccgg ccttccgctc gatggcaatc ggagtgatgc
     2521 tggcggtcgc cttcgtgctg gccgcgtcgc tgaccctgtt gccgggagtc ctcggcaagc
     2581 tgggccaccg ggtcaacaag ttgagcctcc cctgggttca caccggtgag caccgctcgc
     2641 ccaagtttgc cgcctggggc gagaggatct ggaaacggcc gctcctcttc ggtgttccgg
     2701 ccctgctgat cctgctggca ctctgtgtgc cggtgttctc gctggatacc ggcatgccct
     2761 cgatcaaggt cgtgcccgaa ggtgacggcg cgcgggtcgg ctacgaacag gttgccgatt
     2821 ccttcggcgc cggtgccccg ggcacccttc aggtggtcgc cccgaacagc gaagtcgctc
     2881 aggcgaccac ggcgctcaag tccgacaagg gaatcgccgg cgtgatggtt gccggccaaa
     2941 gcccccaggg ctacacgctg atcgcggcca ccccaacggt cgatccctcc gaccccgcga
     3001 tggggccgac gatcgaccgg gtccgggacg agttgccggc cggggccctg gtcggtggcg
     3061 cagcggccga gaaccatgac ctcgagaacg ccctcggtga caccaccgcc ccggcgatcg
     3121 cgattgtcat gaccctgggc ttcctgctcc tgctgatcgc cctgagggca ccgatcctgg
     3181 ccgcaatcgc ggttctgacc aacctgctgg ccgttggcgc cgcctttggt gtcgcccagc
     3241 tgatcttcca ggaaggccat ctgaccggct tgctcggctt cgagtcgcag ggcttcctgg
     3301 acgcctgggc accggtgttc ttcttcgcga tggtgttcgc catatcgatg gactactcgg
     3361 tcttcctgct gagttcggcg agggaacact gggaaaagtc cggcgatgcg aaggaagcgc
     3421 tgattggtgg tctggcccac tccgggcggg tggtgttcgc cgccgccgga gtgatggtcg
     3481 cggtgttctt caccttcgcc ctgtccgggc cactgcctcc ccgcgagatg ggaatcatcc
     3541 tcggtgtggc agtgctgctg gacgccgcgc tggtccggct gctgctgctg ccggtcctga
     3601 tgcgactggc cggcacgaag ttgtggtgga taccgacctg gatcgaccgc cgcctgccca
     3661 aggtgtcctt cgggcactga gttcaggtaa caagcgaaac cccgggggga ggggcgggtt
     3721 gtggaagcat gacccgtctc tccccccttt tcgttgcgtt attcgccctg gtcctggtca
     3781 cgacaccggc ccgggcagtt gagtttcccg agggagtcga ggcgggcgca ctcaccgacg
     3841 cgccgctgct cgcctcgaac tgcgaccgga tcaaggagga cggcgtaaag acaacggtca
     3901 tcggggcagt gatgacgagg atcgtcaaga gcccggggac ctacgacttc agcacgcttg
     3961 atggccaggt caacgcggcg atcggctgcg gcatcgtccc ggcaatccgg gtcttcatcc
     4021 agccgtcgag cagcgacgac cgcggcatgc cggacgacct cgatgcgtac agtgcgttct
     4081 tcggggcgct ggtcggccac atgaaaggca gggtcacgcg cttcggggtc gagaacgagg
     4141 tgatcgcccc gacccactgg accgacaccc cggccgagta cttccagctc ctcaagatcg
     4201 ccgcggacgc cgctcacgcg gcgaacccgg atgccatcct cctcgactcg gtcagttcca
     4261 gcggcggctt ctcgatcatc gaggcacgca aacagtggct ggccggggac ctcgacgggg
     4321 cgctggcgac catccaggaa agccagacca atgacctcgg cggcggcccc tacctgacct
     4381 cgacctccca ggtcccggcc tacctgtcaa gtgccaaggt cctgaagatg caggagttct
     4441 acgaccagct ggtcgcccac aagaatctga tcgatgccct gcaactccat tactacggac
     4501 ccgcctcgag catcccctcg acgatcgcga tggtccggaa cgacggtttc aacatgccga
     4561 tcgagatctg ggagctcggc caccggtacc tcgacggccg gcccttcgag cagcaggacc
     4621 atgccgacga gtcgacccgc ctgatcgcaa cggcggtcgg cgagggcgcc cgttacgtgg
     4681 ttctccagca gtacttcgac aagctcgcca acaagatgta cgggctgatc gacggcgacg
     4741 ggaattcccg ccaggcgcgg ttcgccgttc gcaacacact ttcaatgctg agggacacca
     4801 cctccgccag gcggctccgg gccggctcgg gaaccgacgg atacaggttc gaccggcccg
     4861 gaggctggcg gcgggtcttg tgggcaacgg gtgccgccgt aagaccagga aaggcgctcg
     4921 ggatccgctc gaccacggtg gcagtcaccg gcaaccaggg cggccgctcc cacgagcgca
     4981 tttcttcggt gacggtcggg aagtcgcccc ggtggttcga gccggacatg ctcgagatct
     5041 ggcgccagaa ggccaggcgg aagggattcc tgcgagttaa ggcacactgt cctgcggctt
     5101 cgccgacccg gttctgccgg ggctgggtca agttccgggg cggcaaggga aaacgcaagg
     5161 ttgtcgcggc ccgcaagtac aagatcggcc gcggcgcgag ccgcgtgatc gacgtacggc
     5221 gcaggaaaca acggaagcgg gtcaggccac aggtgatctt cgccacgccg ttcagttgcc
     5281 cgggcaagct gaaccactgc cgcagttggc agaacctccg ctagccccca cgcctctcga
     5341 tcaattcgcg tattcctcga gcacgaccac gcagccgcgc tcgaggatgt ccaggacctc
     5401 gtcgaagccg tcctcgccac cgaaatacgg gtcggggacg tcgacctccc cctcacccgc
     5461 catctcccgg aacagccgga ctttcccggg gtcggcacct tccatggcca gcaggttggc
     5521 gtggttggaa cggtccatgg ccaggatcag gtcgaattcc tcgaagtcgg aagatcggac
     5581 ctgcctcgcg ctgccgctga tctcgacgcc ccgctcgatc gcggccgaga ttgcccgggg
     5641 atcggccgac tcgccgacat gccagtcacc ggtgccggcc gaatcgacct ccaccccttc
     5701 tatctcctga ttcgcgatca atccgcgcat caccgcctcc gccgacggtg agcggcagat
     5761 gttgccgaga cagacgaaga gaatccgcaa ccgctggcct agttggcgta gccgctcagc
     5821 ctgttcaccc ggttggtgag gctgcgcaga ttcttgttga gccggttgat ccgctggctg
     5881 agctcggtgt agtcgttgtc ctgcctggtt tccaccccgg ccagctcgtc ggtcagctca
     5941 cgctggcggc tgctcagagc gttgagctgg ttcctgagct tccggttctg ccggatcgcc
     6001 cgcttgatct gtttgtggtt tccgttgatc ttctgaagga ggttcttctc tccggaagtc
     6061 aactgggaaa cgagcttctc ggcttccgac ttctggtttg cctcgagctg gtcctgcttg
     6121 gactgggcct gctggaactt gtcctggacc agcgtctgga tctgcgacgt cgtgttggtg
     6181 tcggagtcaa cggcgttctt cgcgttgaac gcggtgatcg tgccgaggag ccccagaagg
     6241 attcccaccg agccgatcac tattgccaga gtcgccctgg ttctgccggt catggcggtg
     6301 accctagagc aaccgccgct tttcggcttt tcagagcttg atcaggagga tcgtgttcag
     6361 cgtgaacagg atcgcggtaa cgaccgtggc gcggtcgaga ttctgctcga ccatcgagcc
     6421 gccgccgatc gagctgccgc caccaccgat gccgaaggcg ccggaaaggc ccgcgtcctt
     6481 gcccgagtgc aggagcacca ggaagatcag gagcacggaa agcagaacct ggatgatggc
     6541 catgatcgtg tacatcgact gaccagtcta gctttcttcg cccttgagcg cctcgaggcg
     6601 gtc
//
LOCUS       DAOTIP010000017         6516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_14061_length_6516_cov_4.028943, whole genome shotgun sequence.
ACCESSION   DAOTIP010000017 DAOTIP010000000
VERSION     DAOTIP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14061_length_6516_cov_4.028943"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="P5138_00700"
     CDS             complement(<1..470)
                     /locus_tag="P5138_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV59126.1"
                     /translation="MVETATRRRLPRAEREARMLDAAEAVFSDQSFASASMEQIAADC
                     GITKALIYQYFGSKEGLYVATVERSRKELFDRMEAEAAEAGSPEERLNRLTSIYLEDL
                     IALKGRPILLYGDAPPAAVNEMRERNAKSLARILRIDFPGAGELELELAANAIVG"
     gene            complement(463..1812)
                     /locus_tag="P5138_00705"
     CDS             complement(463..1812)
                     /locus_tag="P5138_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRV59127.1"
                     /translation="MGIGDFRRGLLLCLAFAAGLLLTVAPGAGAKVKVLKSEKLDSRL
                     TELTLSTPNVDGPTGVRVLLPAGYGSSSRNYPVLYLLHGAIDDYRSWTDKGDAEALTA
                     GLPLIVVMPDSGPSDGYTNWDNPGPDGQPAWERYHIEDLIPFIDQRFRTRADRAGRAV
                     AGLSMGGYGAMIYAAKHPDLFTAAAAFSGAVNLTNPLLLSISTPTARGDYATHKLRFE
                     SDNPLSLAENLGGIDLTLRTGDGSSGGPLDPDGGFDIVESVVHASNVSLHDRLGELGI
                     GHVWEDYGPGNHTWPYWQRDLKKTLPGLMKRFRDRTPAPKRITFKSADSSYGAYGWSV
                     KINRKAGEFSMLSDAGRNGFGISGSGRATVRTAPLLRKCRRFSVTIRDVSGKRKTVRH
                     TGRKRRLEVKVNLGPANPFEQFTPEDEAAGHHSASANVSFKLLGKDRCGKKKRGGHG"
     gene            complement(1938..2327)
                     /gene="rplL"
                     /locus_tag="P5138_00710"
     CDS             complement(1938..2327)
                     /gene="rplL"
                     /locus_tag="P5138_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRV59128.1"
                     /translation="MATSTEEWIDELKGISVLELSERIKALEEEFGVSATAVAAAAPA
                     AGAAGGDASDATDEGSSTVDVVLTEVGDKKVPVIKVVRAATGLGLKEAKALVDEAPKP
                     VKEGIEKDEAEKLKAELEEAGASVELK"
     gene            complement(2334..3089)
                     /gene="rplJ"
                     /locus_tag="P5138_00715"
     CDS             complement(2334..3089)
                     /gene="rplJ"
                     /locus_tag="P5138_00715"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRV59129.1"
                     /translation="MNREEKAALIEEIGAEIESSEAIFAVDYRGISVTQAAELRNGLR
                     EADATFKVVKNRLTKLAAEKAGATELNELLQGPTALTFVKGDTAAAAKAITSFNKEHE
                     VLTYKGGVMGDTILDEGRFIAISKLPSRDVLNGQLAGIVASPLVTLTRGLGSMISGLA
                     IALQQVADQGLVGGTAEAPAEEPAAEEAAAEEAPEAEAEAPAEEATEEEAPAEEAEAP
                     AEAEAEEPAAEEAPAEEAEASDEDKAESEENEE"
     gene            complement(3302..4720)
                     /locus_tag="P5138_00720"
     CDS             complement(3302..4720)
                     /locus_tag="P5138_00720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRV59130.1"
                     /translation="MESNVRVVGKRHRDRDFVLRRILLAGDLAALCLAFSFSTALGGA
                     RESELQYGLWFALTLPGWALLFRAYGLYSRPVRSFEPTHLDDLSRLFHAMVLGTLALW
                     VFYKLIPPVKQLNLEEVTVFWLSGLTLTALLRRAMRAINLKRQGAERVLLVASARDAL
                     VMERKLGNHPEYEMKLVGVAADGRPDEALGLPLIDSMDEVEAMLSTNQVDHVIVRLDS
                     PILPAGKAEDLMYRCFRYGVRFGAYPGPRSLLLPGVQLNHIEGMGILTHDPPTLSRSD
                     RLVKRLLDIVVSSAMLVIVTPLMVGLAIAIKVDSKGPVLFRQLRVGRDGVRFKLNKFR
                     TMVPEAEAMTAELMEKSLDPNWLFLEEDPRITAVGRFLRRTSLDELPQLWNVLRGEMS
                     MVGPRPLSERDDERLRGWERHRLDLVPGVTGNWQVLGRTSIPFKEMVEIDYAYVTGWS
                     LWLDVKILIRTVPTVLSRRGAN"
     gene            4899..5945
                     /locus_tag="P5138_00725"
     CDS             4899..5945
                     /locus_tag="P5138_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRV59131.1"
                     /translation="MHSGRKNAIGLGVIGLGYWGPNHIRVVQESERTNLNWICDSNQT
                     RLTQMSSKTRAATTTQVEKVFNDPATDAVIISTPISTHYELGMSALEAGKHVLIEKPI
                     AGSQAEADDLINAAEQNGLVIMCGHTFLFSPPVVAIKEIIRRGELGDIYFISSSRVNL
                     GLHQSDASVLWDLAPHDFSIILNWIDEVPVSVSSVGRDSIGSGVFDVAFTNLVYESGL
                     IANVELSWLAPSKLRRTTVVGSKKMIVYDDTAPEQVRVFDRGVNLDNPETFGAHQLTY
                     RAGDIVSPHLASSEPMSVQLNSFVDAVVSGNYPAEYIRIARDVVGLIEAAERSLTNLG
                     VRTEYRAMQTAQAS"
     gene            complement(5957..>6516)
                     /locus_tag="P5138_00730"
     CDS             complement(5957..>6516)
                     /locus_tag="P5138_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRV59132.1"
                     /translation="SFYPGKNLGAIGDAGALVTNDPEVADLARHLREHGSTDRRIHTY
                     IGYNSRLDSIQAAALRVALPHVDDWTQARREIAGIYQTSGLGEIVGLPRETEGSRSCY
                     HQYVISSPRRDELQDNVEKAGIETRIYYAPALPHQPAMKEFAPTGPLRVTTRYEETAL
                     ALPMGESLGATRAGLVVDAIRSALD"
BASE COUNT         1303 a         2010 c         1841 g         1362 t
ORIGIN      
        1 ccgacgatcg cgttggcagc cagctcgagc tcgagttccc ccgcgcccgg aaaatcgatc
       61 ctgagaatgc gagccaggga tttggcgttt cgctccctca tctcatttac cgcagcgggg
      121 ggcgcgtcac cgtagagcag gatcggacga cccttgagcg caatcagatc ttccaggtag
      181 atcgaggtga gccggttcag ccgttcctcc ggggaaccgg cttctgccgc ctccgcctcc
      241 atacggtcga agagttcctt ccgagaccgt tcgacggttg cgacgtaaag cccctctttg
      301 gacccgaagt actggtagat cagggccttg gttatgccgc agtcggcggc gatctgttcc
      361 atcgaggcgc tggcgaagct ctgatcgctg aagaccgctt cggccgcatc gagcatgcgg
      421 gcctcccgct cggcccgggg cagcctcctt ctggtggccg tctcaaccat gcccgcctcg
      481 ctttttcttt ccgcagcggt ctttgccaag cagcttgaag ctgacgtttg cgctcgcgct
      541 gtggtggccg gccgcttcgt cttccggggt gaactgttcg aaaggattgg ccggaccgag
      601 gttgaccttc acctcgagcc tgcgcttcct gcctgtgtgc cgcacggttt tccgcttgcc
      661 cgaaacatca cggatcgtga ccgagaagcg gcggcatttg cgcagcagtg gcgcggtgcg
      721 gaccgtagcc cggccgctcc cggagatccc gaatccgttc ctccccgcat cgctcagcat
      781 cgagaactcg ccggccttgc ggttgatctt caccgaccaa ccgtaggcgc cgtaactgga
      841 atcggcgctc ttgaaggtga tccttttggg tgccggggtc cggtcgcgaa atcgcttcat
      901 caggccgggc agggtcttct tcaggtcgcg ctgccagtag ggccaggtgt ggttgccggg
      961 cccgtagtcc tcccagacat gccctatccc gagttcgccg agccggtcat gcagcgagac
     1021 gttgctggca tggacgaccg attcaacaat gtcgaagcca ccgtcagggt cgagtggccc
     1081 accggaactc ccgtctccgg tgcggagggt gagatcgatt ccgcccaggt tttcggccag
     1141 gctgagcggg ttgtctgatt cgaagcggag cttgtgggtt gcgtagtccc cgcgggcggt
     1201 cggggtggag atcgaaagca gcagcggatt ggtcaggttt acggcaccgg aaaacgcggc
     1261 ggcggcggtg aacaggtccg ggtgctttgc cgcgtagatc atcgctccgt acccgcccat
     1321 cgaaagtccg gcgaccgccc ggccggcccg gtccgccctg gtgcgaaagc gctgatcgat
     1381 gaaggggatc aggtcctcga tgtggtagcg ctcccaggcc ggctggccgt cggggcccgg
     1441 gttatcccag ttggtgtatc cgtcgctcgg tcccgagtca ggcatgacga cgatcagcgg
     1501 caggccagcg gtcagtgctt cggcgtcgcc cttgtcggtc caggatctgt agtcgtcgat
     1561 cgccccgtgg agcaggtaga ggaccgggta gttccgactc gaggacccgt aaccggcggg
     1621 caggagaacc ctcaccccgg tcggaccatc cacgttcggg gtcgaaagcg tcagttcggt
     1681 cagcctcgaa tcaagcttct cgctcttgag caccttgacc ttcgccccgg cccccggcgc
     1741 aaccgtcaag agcagcccgg cagcaaaggc caggcaaagc agcagtcccc gtcgaaagtc
     1801 tccgattccc atttctactc ctcgatccct gaaataactg acggttagtt atatacggac
     1861 caacccccag atgcaacccc gcaacaagaa agggcgcccc gaggagcgcc ctttcttcga
     1921 aacttcggta cgaactctta cttgagttcg accgaagcgc cggcctcttc gagctcggcc
     1981 ttgagcttct cggcctcgtc cttctcgatg ccttccttga cgggcttcgg agcctcgtct
     2041 accagggcct tggcctcctt gagaccgagt ccggtggcgg cacgaacgac cttgatgacc
     2101 gggaccttct tgtcgccgac ctcggtcaga acgacgtcca cggtcgagct gccctcatcg
     2161 gtagcatcac tggcgtcacc accggcagcg ccggcggcgg gggcggcagc ggccacggcc
     2221 gtagccgaaa cgccgaattc ctcttcaaga gccttgatgc gctcgctcag ctcgaggacg
     2281 ctgatgccct tgagctcgtc aatccattct tcggtgctgg ttgccatttc tggttactcc
     2341 tcgttttctt ctgattcagc tttgtcttca tccgaagctt cggcctcttc ggccggtgct
     2401 tcttctgcgg cgggttcttc agcttctgcc tcggcaggcg cttcggcttc ttcagccgga
     2461 gcctcctcct cggtggcctc ttcggccgga gcttctgcct cggcttcagg agcctcttcc
     2521 gcagcggctt cctcggcggc cggctcttca gccggagcct cagcggtgcc gcctaccagg
     2581 ccctgatcgg ccacctgctg gagggcgatc gcgaggccgg agatcatcga tcccagaccg
     2641 cgggtaaggg tgaccagcgg gctggcgact atgccggcca gctgcccgtt gaggacgtcg
     2701 cgcgaaggaa gcttcgagat tgcgatgaaa cggccctcgt cgaggatcgt gtcacccatg
     2761 accccaccct tgtaggtgag gacctcgtgc tccttgttga agctggtgat cgccttcgcc
     2821 gcggcggctg tgtcgccctt gacgaaggtg agcgcggtcg gcccctggag cagttcgttc
     2881 agctccgtgg cgccggcctt ctcggcagcg agcttggtga ggcggttctt gacgaccttg
     2941 aaggtcgcat ccgcttcccg cagcccgttc ctgagctcag ccgcctgggt taccgagatg
     3001 ccccggtaat ccacagcgaa gatcgcttcg gatgattcga tctctgcacc gatctcttcg
     3061 atcagcgcgg ctttttcttc acggttcatg tttgcctgac ttgtctttgt ctgacctgtt
     3121 tctgtcgcag acaacccggg caaagaaaaa cccgctcaca ggcgggcaca ggatcaggcc
     3181 acaaagggcc tgaatggctc acctgcgctg ggaattacgg gctatcgccc gaccagcggt
     3241 ctacggcacg aaaggcatct taccgagatt gggcacaact ggtctgccga gccacgcacg
     3301 atcagttcgc tcctcgtcga ctgagcacgg ttggaacagt tcggatcaaa atcttcacgt
     3361 ccagccacaa agaccagcct gtcacgtacg cgtagtcgat ctcaaccatt tccttgaacg
     3421 ggatggaagt tctgccaagg acttgccaat tgccggtgac cccagggacg agatcgagtc
     3481 ggtgcctctc ccaacctcga agcctctcgt cgtctctttc gctgagcggc cggggaccca
     3541 ccatgctcat ctcacccctc aacacattcc aaagctgagg cagctcgtcc aaactagtgc
     3601 gacgcaggaa ccggccgact gcggtgattc gcgggtcttc ctctaagaaa agccagttgg
     3661 gatccagact cttctccatt agctcagccg tcatggcctc agcttcaggc accatcgtcc
     3721 ggaacttgtt gagcttgaac cggacaccgt ctctgcccac tcgcaactgg cggaaaagaa
     3781 cgggtccctt cgagtcaacc tttatggcga tggccaggcc gaccatcagg ggcgtcacga
     3841 tgaccaacat cgccgaggaa acgacgatgt cgagaagacg cttgaccaaa cgatcgctac
     3901 gcgacaaagt cggcgggtcg tgagtcagaa ttcccattcc ttcaatgtga ttcagctgta
     3961 ctccaggaag gagaagactc cgtgggccgg ggtaggcgcc gaaccggacc ccatatctga
     4021 agcaccggta catcagatcc tctgccttgc cagctggaag aatcggtgag tcaaggcgga
     4081 cgatcacgtg atccacctga ttcgtcgaca acatagcttc aacttcgtcc atcgaatcaa
     4141 tcagcggcag cccaagcgcc tcatccggcc gaccatcggc ggctacgccg accaacttca
     4201 tttcgtattc ggggtgattt ccaagtttgc gctccattac caaggcatcg cgggcactcg
     4261 cgacgagtag cacccgctcg gcgccctgac gcttcaggtt gattgcccgc atggccctcc
     4321 tgagcaacgc agtaagggtc aaaccggaca gccaaaagac ggtgacctct tcaaggttga
     4381 gctgtttgac gggcgggatc agtttgtaaa agacccaaag cgcgagagtg ccaaggacca
     4441 tcgcgtggaa gagccgcgag agatcgtcca ggtgggtcgg ctcaaagctc cggactggcc
     4501 ggctgtaaag accgtaggcc cgaaaaagca aggcccaccc aggcagagtc agagcgaacc
     4561 acaacccgta ctgaagctca ctctcacggg cccctccgag cgctgttgag aaagagaaag
     4621 ccagacaaag cgcggcaaga tctcccgcca aaaggatccg ccgcaatacg aaatcccgat
     4681 cgcggtggcg tttccctacg actctcacgt tggattccat tcctaacccc ttagtactaa
     4741 ccactcaaat ccccactcct ggaaccggcg caaggaaaag aaatttcctc gactccggca
     4801 gcgatcgatt ctggtcgcta tcctagcgac aggcggactg gctatgaagg tacttgtccg
     4861 ctaggagccg actcaaatta tttaggggat ctagacagat gcatagcggg agaaaaaacg
     4921 caatcggcct cggagtgatc gggctcggat attggggacc aaaccatatc cgcgtcgtcc
     4981 aggaatccga acgcacgaat ctgaattgga tttgtgactc caaccagact cgcctgactc
     5041 agatgtcgag caagacgaga gctgccacca caacacaggt ggaaaaagtc tttaatgatc
     5101 cggcgaccga tgctgtgatc atctcgactc caatatcaac tcattacgaa ctgggtatga
     5161 gcgcattgga agccggaaag cacgtactga ttgaaaagcc aattgccgga agtcaggccg
     5221 aagccgatga cctcatcaat gctgcagagc aaaatggact cgtaatcatg tgtggtcaca
     5281 cgttcctctt tagccctccc gttgtggcaa tcaaagaaat aatccgccga ggagaactcg
     5341 gagatatcta tttcatttca tcaagccgag tcaatcttgg tcttcaccaa agcgacgcat
     5401 cggtgttgtg ggacttggct cctcatgatt tctcaataat tctcaattgg atagatgaag
     5461 ttcccgtctc agtctcctcg gtcggtcgcg attcgatcgg ttccggtgtc ttcgatgtcg
     5521 cttttacgaa cctggtgtat gaaagcgggc taattgccaa cgtagaactc agttggctcg
     5581 ctccgagcaa actgcggcgt accacagtcg ttggaagtaa gaaaatgatc gtgtatgacg
     5641 acaccgctcc ggagcaagtt cgcgtcttcg accgtggtgt gaacctcgat aacccggaga
     5701 ccttcggcgc acatcaactc acttatagag ccggcgacat agtgtctccc catctcgcct
     5761 ccagtgaacc gatgagcgtg caactcaata gtttcgtcga tgcggttgtc tctgggaact
     5821 accctgctga atacatccgc attgcccgag acgtagtcgg cctcatcgag gctgccgagc
     5881 ggtcactcac taatctcggc gtccggaccg aataccgggc aatgcaaact gcacaggcgt
     5941 cctagatcca cacacttcaa tccagggcgc ttcgaatcgc gtcaaccaca agaccggccc
     6001 tggtggcgcc caaggattcc cccatgggca gggcaagagc ggtctcttcg tagcgcgtgg
     6061 tgactcggag aggaccagtc ggagcaaatt ccttcatcgc tggctggtga ggaagtgccg
     6121 gtgcgtagta gatcctggtc tcgattccgg ctttctcgac attatcctga agttcgtccc
     6181 ttcgcgggct tgaaatcaca tactggtgat agcaggatct acttccttcg gtctccctgg
     6241 gcaaaccaac tatctctccc aagcctgatg tttggtaaat ccctgctatc tcccgccgag
     6301 cctgcgtcca atcatcaacg tgtggaagag ccaccctcag agccgctgcc tgaattgaat
     6361 ccaaccgtga attgtaccca atataagtgt gaatacgtct atctgtggaa ccgtgctccc
     6421 tcagatgtct tgctaggtcg gcaacttcgg gatcgttggt tacgagagct cccgcatctc
     6481 cgatagcgcc caaattcttt ccaggataaa aactaa
//
LOCUS       DAOTIP010000018         6416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_14345_length_6416_cov_3.187235, whole genome shotgun sequence.
ACCESSION   DAOTIP010000018 DAOTIP010000000
VERSION     DAOTIP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14345_length_6416_cov_3.187235"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="P5138_00735"
     CDS             complement(<1..1038)
                     /locus_tag="P5138_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRV59133.1"
                     /translation="MTSSPASSSGSRPAGSRRRQSRFPRRRHIGMRVWLAAGFTLVCF
                     LTAGILYSFITSKSQDVLNERSTELAVGRTVRLADRLSQPDVDAKAELEAANGEGYTA
                     WFFDSDGNLVAPENPRAEFSSSKPYQETLDLALEGGRPSRDLKGGEIAVVGIPVTDFG
                     TIEGAVVGRYSRPIVIRSAIDKLRSESLRAALIAILIGTLVGVGIASVITGRLKGIAA
                     KADDLASGNFDVRLPIRGRDEIGDLARSLDSMRAALKESFGVLTADRDKLAAIFDGLG
                     DSVMVIDNNGEVRFHNSAANALLEGGGRAPEPMMSSVRRAAAEGFAAHPALRMGDRAY
                     ALQARSLPDEGA"
     gene            1150..1866
                     /locus_tag="P5138_00740"
     CDS             1150..1866
                     /locus_tag="P5138_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV59134.1"
                     /translation="MKKLVFIPARNEENSIEAVISDAREHVPGADIMVVDDGSTDRTA
                     ALARRAGALVASLPFNQGVGAAHQTAYIFALRHGYEICGQLDGDGQHPASEVARLIEE
                     VEKGADLAVGSRFAGNVNPDIPEYRSTFARRIGQRMFSLLVSTSTRQTFTDTTSGMRA
                     LNEKAMHLFADRYSSEFAEVESLQQAVRAGLVVKEVPVHMLAREHGRSFIGPLTSAYY
                     IFKTLIVLLIGQLRPRQVEE"
     gene            1878..2279
                     /locus_tag="P5138_00745"
     CDS             1878..2279
                     /locus_tag="P5138_00745"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HRV59135.1"
                     /translation="MTRLLAESEPVVRLTPQARILAAVLAIAFMLLILDLIRRDKLLE
                     RYSIVWFLLGIGMLLGAIFPVALEWLAKAMGVRDVTIALFSVTLLILLALSLNFSVIA
                     SRQSKQITRLAQQSAIDGVEEQTEEVEGRDR"
     gene            complement(2304..3404)
                     /locus_tag="P5138_00750"
     CDS             complement(2304..3404)
                     /locus_tag="P5138_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HRV59136.1"
                     /translation="MITLEEATSHQPRAAAELKAALGQGPSHAYLFQGPSGSGKSKAA
                     RAFAAEILTTGSDDPEDSRRRALLEPSPHPDLVWLAPKGMSHAVQEIRERVIHQAPLS
                     PFEGTSRVFVIEAAEALNEESQNAMLKTLEEPPPHAHLILLSSESEGVLPTIASRCQL
                     IEFGSLSPEVIEEELDGLATPETLHSAAKLAAGDLDRARFLAGKRGGELRVSIERLMT
                     CALEDDFTASPWLELLSQAKSAGEHSGELVQAEFDEEKEEGIKHTKTEMEEAVRRAQR
                     KTRTGMLDLGLHLAAAWARDWATVIAGAPILAFNQDRLEVLEAQAEGIELGQAREAVS
                     RIQETRRSFELNVSEELALEALAFRLEKTLRD"
     gene            complement(3433..4026)
                     /gene="tmk"
                     /locus_tag="P5138_00755"
     CDS             complement(3433..4026)
                     /gene="tmk"
                     /locus_tag="P5138_00755"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRV59137.1"
                     /translation="MFITLEGVDGSGKTTQAALLARAMGTRAMTLREPGGTEAAERIR
                     QLLADPDTPLDPIAELMLFCAARADLVSRVIRPALEVGRIVICDRFTDSTIAYQGVAR
                     DLGIVRVRELSEVATDGLTPDLTLLLRIDPAFGLDRAQSDDRFEAEGVRFQQRVAEAY
                     EDIARREPSRVIPIDATGSPEEVHKLIMAAVDARLPA"
     gene            complement(4036..6384)
                     /locus_tag="P5138_00760"
     CDS             complement(4036..6384)
                     /locus_tag="P5138_00760"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HRV59138.1"
                     /translation="MRLIITEKNNSAQKIAEILSNGAATEKKSFTVPVFRWEDSDGET
                     AVIGTGGHFVGREFPQEKEYKQWKLDLIPGLIDAPLETGPIDGKKNVIKAVQKEAKQA
                     DSLVIGTDFDREGELIGLEALEVCLEVNPGLEPTLKRARYSALTKEEIEGAFDNLDEL
                     SYPLANAAGARQDIDLIWGAAFTRAVSLVAKAYGANFLSVGRVQSPTLGLIVERELER
                     RAHVAKPFWELFAKFEHPSGHSFEAHHATDKFWDKGEADAALKGTASPGAVKAVTSRK
                     STSKPPTPYNTNSFQVDASSRLGITPKRAMDLAQDLYDDGFISYPRTDNTIYPDSLPL
                     EKTIASLVKIKDFAAAAPILDKPLHPTQGKKFDAAHPPIYPTHALYPSALDGPKARVY
                     ELIVRRFLATFGEPMVTESTRADIEAGSETYFVRGKVVLDPGYAGIYTYARSADEEIP
                     ALEEGQQVALDGAPWLIDKETQPPARLSEAKLVQLMDEQGLGTKATRPDIIQKLYNRR
                     YVRNHPPEPTATGIAMYEAFKEYVPDMATSEMTASLEGEMDEIAEEKMTKDEVVEDSR
                     KLVHKTFKDLLEGEEALSKMIWEGMDRDRILGPCIVCQEAGRTKPDGSPNMLRIIRAK
                     KSGKSFVGCTGWEADNPESCDQTFPMPQPNFYEVTPLEENCSVCHRTPRVNVKTRGRA
                     GRPWKLCFNDDCPTMVEMREKKAERLAAQEAAKKAKEAKEAEEKASGKKKPAKKSSGK
                     KSSKKATTVAARIASPPDLGTRRVRKAGSRKGGSGGASKRKG"
BASE COUNT          960 a         2111 c         2161 g         1184 t
ORIGIN      
        1 cgctccctcg tccggcagcg accgggcctg gagggcgtag gcgcggtccc ccatccggag
       61 ggccggatgg gcggcgaatc cctcggcggc agcccgcctg accgaggaca tcatcggctc
      121 cggggcacgc cccccgccct cgagcagggc gttggcggcg gagttgtgga agcggacctc
      181 gccgttgttg tcgatcacca tgaccgagtc cccgagcccg tcgaaaatcg ccgcgagctt
      241 gtcccggtcg gcggtgagca ccccgaagct ttccttgagg gcggcccgca tcgagtcgag
      301 cgagcgggcg aggtccccga tctcgtcgcg gccccggatc ggcagtcgca catcgaagtt
      361 gccagaagca agatcatcgg ccttggcggc gattcccttc aggcggccgg tgatcaccga
      421 ggcgatcccc accccgacca gggtgccgat caggatcgcg atcagtgcgg cacgaaggct
      481 ctcggagcgg agcttgtcga tcgccgagcg aatcacgatc gggcgggaat agcgaccgac
      541 gaccgcgccc tcgatcgtgc cgaagtcggt gaccgggatg ccgaccaccg cgatttcgcc
      601 gcccttcagg tcacgcgagg gcctgccccc ttcaagggcc agatccaggg tctcctgata
      661 cggcttgctc gaggagaact cggcccgggg gttttcgggg gcgacaaggt tgccgtcgct
      721 gtcgaagaac caggcggtgt acccctcgcc gttggcggct tcaagttccg cttttgcgtc
      781 cacatcgggc tggctcagac ggtcagccag acggacggtg cgaccgaccg ccagctcggt
      841 cgagcgttca ttgagtacgt cctggctctt gctggtgatg aacgagtaga ggatgccggc
      901 ggtcaggaag cagaccaggg tgaagccagc tgccagccag acgcgcatgc cgatgtgtcg
      961 gcggcgcggg aaccggctct ggcgcctgcg gctgccggcc ggtctgctcc cggaactgct
     1021 ggcgggactg ctggtcattg gggcggaccc tatttcatgg ctccatggct gaaaaggccc
     1081 gggcgagatt cgaccaccgg aagaccgatt cgaccgcctg acgaggcgtg gcatgggaga
     1141 atcgcgatcg tgaagaaact ggtcttcatc ccggcgcgga acgaggaaaa ctcgatcgag
     1201 gcggtgatca gcgatgcccg cgagcacgtt cccggcgccg acatcatggt cgtcgacgac
     1261 ggctcgaccg accgcaccgc ggcgctggcc cgccgggccg gggccctcgt cgcctcgctt
     1321 ccgttcaatc agggggtcgg cgccgcacac cagaccgcct acatcttcgc cctccgccac
     1381 ggctacgaaa tctgcggcca gctcgacgga gacggccagc atccggcttc cgaggtcgcc
     1441 cgcctgatcg aggaagtcga aaagggtgct gacctggcgg tcggttcgcg ctttgccggg
     1501 aacgtcaacc cggacatacc cgagtaccgg tcgaccttcg cccggcgcat cggccagagg
     1561 atgttcagcc tgctggtctc gacctcgacc cgccagacct tcaccgacac aaccagtggc
     1621 atgcgggccc tgaacgaaaa ggcgatgcat ctattcgccg accgttactc atccgagttc
     1681 gccgaggtcg agtcgctgca gcaggcggtt cgggccgggc tggtcgtgaa ggaggtgccc
     1741 gtccacatgc tggcccgcga gcacggccgg tcgttcattg gcccgctgac ctccgcgtac
     1801 tacatcttca agaccctgat cgtgcttctg atcggccagc tccggcccag acaggtggaa
     1861 gagtgaacct gactgccatg acgaggcttc tggccgaaag cgaaccggtg gtccggctca
     1921 ccccccaggc ccggatactc gccgcggtcc tggcgatcgc attcatgctc ctgatcctcg
     1981 acctgatccg tcgcgacaag ctgctcgagc gctattcgat cgtctggttc ctgctcggga
     2041 tcggcatgct gctcggcgcg atcttccccg tcgccctcga gtggctggcc aaggcgatgg
     2101 gggttcgcga cgtcacgatc gccctcttct cggtcaccct gctgatcctg ctggccctct
     2161 cgctcaattt ctcggtgatc gcctcccgcc agtcgaagca gatcacccgc ctcgcccagc
     2221 aatccgcgat cgacggggtc gaagaacaaa cagaagaggt cgagggccgg gaccggtagg
     2281 gcgtcccggc tcacggaccg gattcagtca cgaagggtct tctcaagccg gaaggccaag
     2341 gcctcgagag caagttcctc gctgacgttg agctcgaagc tgcggcgggt ttcctggatc
     2401 cggctcaccg cttcgcgggc ctgaccgagc tcgatccctt cggcctgggc ttcgaggacc
     2461 tcgagacggt cctggttgaa ggcgaggatg ggggcgccgg cgatcaccgt cgcccagtcc
     2521 cgggcccagg cggcggcgag atggagtccg aggtcgagca tgccggtgcg ggtcttgcgc
     2581 tgggcccgcc gcaccgcctc ctccatctcg gtcttcgtgt gcttgatgcc ctcttccttc
     2641 tcctcgtcga actcggcctg gaccagctcg cccgaatgct cgccggccga cttggcctga
     2701 ctgagcagct ccagccacgg cgaagcggtg aagtcatcct ccagggcgca ggtcatgaga
     2761 cgttcgatcg agaccctgag ctcgccgcct cgcttgccgg ccaggaaacg agcccggtcg
     2821 agatcaccgg ccgcgagctt ggcggcggag tgaagcgttt cgggggtggc gaggccgtca
     2881 agctcctcct cgatcacctc gggtgaaagc gacccgaact cgatcagctg acaccgggag
     2941 gcaatcgtcg gcaggacacc ttcactctcc gaggagagca ggatcaggtg agcgtgaggc
     3001 ggcggctcct cgagcgtctt cagcatcgcg ttctggcttt cctcgttcag cgcctcggcc
     3061 gcctcgatca cgaagacccg tgaggtccct tcgaaggggc tgagcggggc ctggtggatc
     3121 acccgttccc ggatctcctg gaccgcgtgg ctcatgccct tcggggcaag ccagaccaga
     3181 tcgggatgcg gcgaaggttc cagcagggcc cgacgccggg aatcctcggg atcgtcgctg
     3241 ccggtggtga ggatctcggc ggcgaaagcc cgggccgcct tcgacttgcc ggaaccggaa
     3301 ggcccctgga agagataggc gtgagaagga ccctggccga gggcggcctt cagttcggcc
     3361 gccgcccggg gctgatggga agttgcctcc tcgagggtga tcacgaagcc agtttcgcgc
     3421 agcgccggga cgtcacgccg gcagcctggc gtcgaccgcg gccatgatca gcttgtggac
     3481 ctcttccggg ctgccggtcg cgtcgatcgg gatgacccgg ctaggctcgc gccgggcgat
     3541 gtcttcgtag gcctcggcca cccgctgctg gaaacggacc ccctcggcct cgaaacggtc
     3601 atcggactgt gcccggtcga gcccgaaggc cgggtcgatc ctgagcagaa gggtcagatc
     3661 cggggtcagg ccgtcggtcg ccacctcgga gagttcccgc acccggacga tgcccaggtc
     3721 ccgggcgacg ccctggtagg cgatcgtcga gtcggtgaac cggtcgcaga tcacgatccg
     3781 gcccacctcc agggccggcc gtatgacccg gctgaccagg tcggcccggg ccgcgcagaa
     3841 gagcatcagc tcggcgatcg ggtccagcgg cgtatcggga tcggcgagca gctgcctgat
     3901 ccgctcggcc gcttcggtcc cgccgggctc gcgcagggtc atcgcccggg tccccatcgc
     3961 gcgggccagt agcgcggcct gggtggtctt gcctgagccg tcgactcctt cgagcgtgat
     4021 gaacacgccc ggccctcagc ctttgcgctt cgaggcgccg ccgctgccgc ccttgcggga
     4081 tcccgctttc ctgacccggc gggtgccgag atcgggcggg ctggcgatgc gtgcggccac
     4141 cgtggtcgcc ttcttcgaag acttcttgcc ggaagatttc ttggccggct tcttcttgcc
     4201 cgaagccttc tcctcggctt ccttggcttc cttggccttc ttcgctgcct cctgcgcggc
     4261 cagcctctcg gccttcttct cgcgcatctc gaccatggtc gggcagtcgt cgttgaagca
     4321 gagcttccat ggccgtcccg cccggcccct ggtcttcacg ttgacccgcg gggtgcgatg
     4381 gcagaccgag cagttctcct cgagcggcgt cacctcgtag aagttcggct gtggcatcgg
     4441 gaaggtctgg tcgcaggatt cggggttgtc ggcttcccaa ccggtgcagc cgacgaagga
     4501 cttgccggac ttcttggccc ggatgatgcg cagcatgttc ggcgagccgt ccggtttggt
     4561 gcgtcccgct tcctgacaga cgatgcaggg gccgaggatc cggtccctgt ccatgccctc
     4621 ccagatcatc ttcgagaggg cttcctcgcc ttcgagcagg tccttgaatg tcttgtggac
     4681 cagcttgcgg ctgtcctcga cgacctcgtc cttggtcatc ttctcctcgg cgatttcgtc
     4741 catctcgcct tcgagggagg cggtcatctc ggaggtggcc atgtccggca cgtattcctt
     4801 gaatgcctcg tacatcgcga tgccggtcgc ggtcggctcg ggtggatggt tccgcacgta
     4861 gcgccggttg tagagcttct ggatgatgtc cggccgggtc gccttggtgc cgagtccctg
     4921 ctcgtccatc agctggacca gcttcgcttc cgaaaggcgg gccggcggct gggtctcctt
     4981 gtcgatcagc cacggcgcac cgtccagcgc cacctgctgg ccctcttcga gcgccgggat
     5041 ctcctcgtcg gccgagcggg cgtaggtgta gataccggcg tatccggggt cgagcacgac
     5101 cttgccgcgc acgaagtagg tctcggagcc ggcctcgatg tcggcccggg tcgactcggt
     5161 gaccatcggc tcgccgaagg tggcgaggaa gcggcggacg atcagctcgt agacccgtgc
     5221 cttgggcccg tccagggcgc tcgggtagag cgcgtgggtc gggtagatcg gcgggtgggc
     5281 cgcgtcgaac ttcttgccct gggtcgggtg aagcggcttg tcgagaatcg gtgccgcggc
     5341 ggcgaagtcc ttgatcttga ccagagaggc aatcgtcttt tccagcggca gcgaatccgg
     5401 gtagatcgtg ttgtcggttc gcgggtagga gatgaaaccg tcgtcgtaga gatcctgggc
     5461 gaggtccatc gcgcgcttcg gggtgatccc gagccggctg gacgcatcca cctggaagga
     5521 attcgtgttg tacggcgtcg gcggcttgct cgttgacttg cggctggtca ccgccttcac
     5581 cgcgcccgga ctggccgtgc ccttcagcgc agcgtcggcc tcgcccttgt cccagaactt
     5641 gtcggtggcg tgatgggcct cgaagctgtg gccgctggga tgctcgaact tggcgaagag
     5701 ttcccagaac ggcttggcga catgggcgcg ccgctccagt tcccgctcga cgatcaggcc
     5761 gagggtcggg ctctggacac ggccgaccga gagaaagttc gccccgtagg ccttggcgac
     5821 cagcgagacc gcccgggtga aggcggcgcc ccagatcagg tcgatgtcct ggcgggcgcc
     5881 ggccgcattg gcgagcggat acgagagttc gtcgaggttg tcgaaggcgc cttcgatctc
     5941 ttccttggtc agcgcggagt aacgcgccct tttaagtgtg ggctcgagac cagggttcac
     6001 ctcgaggcag acttcgagcg cttcgaggcc gatcagttcg ccctcgcggt cgaagtcggt
     6061 gccgatcacc aggctgtcgg cctgcttcgc ttctttctgc acggctttga tcacgttctt
     6121 cttgccgtcg atcgggccgg tttccagcgg cgcgtcgatc aggccgggga tcaggtcaag
     6181 cttccactgc ttgtattcct tctcctgggg aaactcgcgc ccgacgaagt gcccgccggt
     6241 gccgatcacg gccgtctcgc cgtccgaatc ctcccagcgg aagacgggca cggtgaatga
     6301 tttcttctcc gtcgcagcgc cgttcgagag gatctcggcg atcttctggg cggagttgtt
     6361 cttctcggtg atgatcagtc gcattggctg gcgagagtag caggggcgcc gaaaga
//
LOCUS       DAOTIP010000019         6134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_15199_length_6134_cov_3.223392, whole genome shotgun sequence.
ACCESSION   DAOTIP010000019 DAOTIP010000000
VERSION     DAOTIP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15199_length_6134_cov_3.223392"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="P5138_00765"
     CDS             <1..357
                     /locus_tag="P5138_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRV59139.1"
                     /translation="PRVASLCREAMMSIGCIQAQRCHTGGCPAGVATQSRWLMGGLDP
                     DLKSSQAANYIVNLRAELLALSRSCGVSHPALVTADHIEIVSERYGIAPLREVFGYEP
                     DWPLVSAASRELVTEA"
     gene            complement(453..2669)
                     /gene="katG"
                     /locus_tag="P5138_00770"
     CDS             complement(453..2669)
                     /gene="katG"
                     /locus_tag="P5138_00770"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRV59140.1"
                     /translation="MSDQPDAVVGEMNEEEAGGCPVAHDRFPEPSEGGSNTEWWPNRL
                     NLNVLAKNPAERNPMGDDYDYAAEFETLDLDAVKKDIAEVLTTSQDWWPADFGNYGPF
                     MIRMAWHSAGTYRVQDGRGGGGHGQQRFAPLNSWPDNANLDKARRLLWPVKQKYGRKL
                     SWADLIILTGNVALETMGFKTFGFAGGRVDEWEPDEDVYWGPEKEWLGDERYTGERDL
                     ENPLAAVQMGLIYVNPEGPNGNPDPVAAAHDVRDTFRRMAMNDEETVALIAGGHTFGK
                     THGAANPEPYVGDEPEAAPLQEQGLGWISTYGSGSGGDTITSGIEVTWTTTPTKWSNN
                     FFWNLFSFEWELTESPAGAHQWKPVGDAGAGTVPHAHNADERIAPNMLTTDLSLRFDP
                     EYEKISRRFMEDPDEFADAFARAWFKLTHRDMGPKERYLGPEIPAEDLLWQDPVPAVD
                     HELVDEVDIASLKEKILGSGLTISQLVSAAWASAGAYRSSDMRGGANGGRVRLEPQKS
                     WAVNDPANLATVITALEGIQEEFNSSAQGGKQISFADLIVLAGSAAVEEAARKAGREV
                     AVPFTPGRGDATQEQTDVESFAWLEPTADGFRNYLHGDHQLPGEYLLLDRANLLGLSA
                     PEMTVLVGGLRALGANTGGSKTGVLTENPETLTNDFFVNLLSFDTNWKPVDENSTSFE
                     SSNGWTGSRVDLVFGSNSELRALAEVYACDDAADKFVSDFIAAWTKVMNADRFDLD"
     gene            complement(2662..3123)
                     /locus_tag="P5138_00775"
     CDS             complement(2662..3123)
                     /locus_tag="P5138_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRV59141.1"
                     /translation="MSSAAEYESLLRDSELRVTRPRVAVLNAVYDHPHADTTSIIDAV
                     RGELPEVSHQAVYDVLRALTDAGLVRNIQPPGSVARYESRVGDNHHHIVCRSCGAIAD
                     VDCAVGDAPCLTASEDHGFVIEEAEVVYRGLCPDCAAESTHDPSPRKESRV"
     gene            complement(3133..3609)
                     /locus_tag="P5138_00780"
     CDS             complement(3133..3609)
                     /locus_tag="P5138_00780"
                     /inference="COORDINATES: protein motif:HMM:NF039724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6314 family protein"
                     /protein_id="HRV59142.1"
                     /translation="MTVDPVTYFPGKWTFERKIRDRLAMVDGKAHGTATFTIEGGGLR
                     WLESARLEIGEADTRASRELWIRPDPSVSSGWRVHFDDGRPFHPLELGDGEPEVTHPC
                     RDDLYEGSIAIDGENLFRTSWTVRGPRKDQSIETTYRRISESGSPGLYLTDSRKEI"
     gene            complement(3634..5304)
                     /locus_tag="P5138_00785"
     CDS             complement(3634..5304)
                     /locus_tag="P5138_00785"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HRV59143.1"
                     /translation="MTRIALVGMDSEDPAQVTVTGSKSGDHEGQLKPLGPSTEGVTFT
                     PDKPFKAQETVTVSIDGDVDIAGVDPGGEYRFKTASFRTDAALPPKVLPPESRIIKHE
                     TFHSRPDLEPPVVDIKKPAASGSGDGLMFMSPKVDGPMIFDDDGALVYYKPSTEAADF
                     RTQTYNGQPVLTWWEGPFNAGGYTEGTNIIANDKYEEISRVLAGNGYQADLHEFKLTD
                     HGSAYLTAYKTVLVDLTPWGGPKRGAVLDSIAQEVDIASGKVVWEWHSLGNVGLGETE
                     IPFPTSPTEAFDYFHINSINEDKDGNIIISGRNTFSVLKVDKSTGKTIWKLGGKKSDF
                     EMGKGTNFSYQHDALRQDDGTLTVFDNASTYGQEPTYPTSRGLVLDLDEENMTAELAL
                     KPFISPQKLLAPSQGNLQKLPNGNYLVGWGAQPWFTEYTPEGKVLYNGTYYARNSSYR
                     VYRDPWTGIPSDDPAIATEDGGDNKVRVWMSWNGSTETASWRILAGPDEDNLKPVTTV
                     DKDGFETGKTIRSHARFFAVEALDANGKSLGQSQPVSIGQQAGPEGTT"
     gene            5542..5958
                     /locus_tag="P5138_00790"
     CDS             5542..5958
                     /locus_tag="P5138_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HRV59144.1"
                     /translation="MVTLPAEARSLIESDALAHLVTINPDGSPQVTCIWVGLDGDEIV
                     SGHMFHVLKLKNVERDPRVVLSIEARTRNEMNLQEYLVIRGTARVEEGRAAELLQDLA
                     HTYMGPDAVFPPPGVESPGFVLRVTPERLGGVGPWA"
BASE COUNT          960 a         1961 c         2022 g         1191 t
ORIGIN      
        1 ccccgtgtgg catcgctgtg ccgggaagcg atgatgtcga tcggctgcat ccaggcacag
       61 cgatgccaca cggggggatg ccctgcagga gtggccacgc aatcgcgctg gctgatgggt
      121 ggcctcgacc ccgacctgaa gtcaagccag gccgccaact acatcgtcaa cctgcgggcc
      181 gaacttctgg cgctctcccg tagttgcggg gtttcccacc cggccctcgt cacggccgac
      241 cacatcgaga tcgtttccga gcgttacggc atcgccccgc tgcgcgaagt gttcgggtac
      301 gagcccgact ggccgctcgt aagcgcggcc agccgggaac tcgtcaccga ggcgtagccc
      361 gcccggcgaa ggatcgagaa ggctagaagg gccctatacg gacactttca gccttctcga
      421 tcccgacggg ccgggccagt tctggcccgg ggtcagtcga ggtcgaagcg gtccgcgttc
      481 atgaccttgg tccaggcggc gatgaagtca ctgacgaact tgtcggcggc gtcgtcacag
      541 gcgtagacct cggccagggc gcggagctcc gagttcgatc cgaagaccag gtcgacccgg
      601 ctgccggtcc acccgttcga ggactcgaag ctggtcgagt tctcgtcgac cggcttccag
      661 ttggtgtcga aggagaggag gttcacgaag aaatcgttcg tgagcgtctc cgggttctcg
      721 gtcaggacac cggtcttcga gccgcccgtg ttggctccga gcgccctcag gccgccgacc
      781 aggacggtca tctccggggc ggacaggccg agcaggttcg cccggtcgag cagcaggtac
      841 tcgcccggca actgatggtc cccatgcagg tagttgcgga aaccgtcggc cgtcggctcg
      901 agccatgcga atgactcgac gtcggtctgc tcctgggtcg cgtcgccgcg gcccggggtg
      961 aacggaaccg ccacctcgcg cccggccttc ctggctgctt cctcgaccgc ggcactgccg
     1021 gccagcacga tcaggtcggc aaacgagatc tgcttgccgc cctgggcgga gctgttgaac
     1081 tcctcctgga ttccctcgag ggccgtgatc accgtggcca ggttcgccgg gtcgttgacc
     1141 gcccagctct tctgcggctc gagccggacc cgcccgccat tggcgccgcc ccgcatgtcg
     1201 ctggagcggt aggcaccggc cgaagcccag gcggcagaga ccagctgcga aatggtgagt
     1261 ccggaaccga ggatcttctc cttcagcgag gcgatgtcca cctcgtcaac cagctcgtga
     1321 tcgacggccg ggaccgggtc ctgccagagc aggtcttccg ccggaatctc cggaccgagg
     1381 taacgctcct tcggtcccat atcacggtgg gtcagcttga accatgcccg ggcaaaggcg
     1441 tccgcaaact cgtccgggtc ctccatgaac cggcgcgaga tcttctcgta ctcgggatcg
     1501 aaccggagcg acaggtcggt ggtcagcatg ttcggggcga tccgctcgtc cgcgttgtgg
     1561 gcgtggggga ctgtgccggc cccggcatcg ccgaccggct tccactgatg cgctccggcc
     1621 gggctctcgg tcagctccca ctcgaagctg aacaggttcc agaagaagtt gttcgaccac
     1681 ttggtcgggg tcgtggtcca ggtgacctcg atacccgagg tgatcgtgtc gcccccggaa
     1741 ccgctgccgt aggtgctgat ccagccgaga ccctgctcct gaagcggagc ggcttccggc
     1801 tcatcgccga cgtaaggctc cgggtttgcg gcaccgtggg tcttgccgaa ggtatgcccg
     1861 cccgcaatca gcgccacggt ctcttcgtca ttcatcgcca tgcgcctgaa ggtgtcgcgg
     1921 acgtcatggg cggcggcgac cggatccgga ttgccgttcg ggccctccgg gttcacgtag
     1981 atcaggccca tctgcactgc ggccagcggg ttttcgaggt cccgctctcc ggtgtagcgc
     2041 tcgtcgccga gccattcctt ctccgggccc cagtagacgt cttcgtccgg ctcccactcg
     2101 tcgacgcggc cgccggcgaa gccgaaggtc ttgaatccca tagtctcgag ggcgacgttg
     2161 ccggtgagaa tgatcaggtc ggcccaggaa agcttgcggc catacttctg cttgaccggc
     2221 cagagcaggc ggcgggcctt gtcgaggttg gcgttgtcgg gccagctgtt gagcggggcg
     2281 aagcgctgct ggccgtggcc gccgccaccg cggccgtcct ggacccggta ggtgccggcc
     2341 gaatgccagg ccatacggat catgaacggg ccgtagtttc cgaagtcggc cggccaccag
     2401 tcctgcgagg tggtcagcac ctcggcgatg tccttcttga ctgcgtcgag atcgagcgtt
     2461 tcgaactcgg ccgcgtagtc gtagtcatca cccatcgggt tgcgctcggc cgggttcttg
     2521 gcgaggacgt tcaggttgag gcggttcggc caccattcgg tgttactgcc gccttcgctc
     2581 ggctcgggga agcggtcatg agcgaccggg cagccgccgg cttcttcctc gttcatttca
     2641 ccgacaacgg catctggctg atcagacacg ggattccttt cggggggatg ggtcatgggt
     2701 tgattcggcc gcgcagtcgg ggcaaaggcc ccggtacacg acctcggctt cctcgatcac
     2761 gaaaccgtga tcctcggagg ccgtaagaca gggtgcgtcg ccgaccgcac agtcgacgtc
     2821 cgcgatcgcg ccgcatgagc ggcagacgat gtggtggtgg ttgtcgccga cccgggattc
     2881 gtaacgggcg accgatccgg gcggctggat gttgcggacc agaccggcgt cggtcagggc
     2941 ccgcagcacg tcgtagaccg cctgatggga aacctcggga agctcgcccc gcaccgcgtc
     3001 gatgatcgag gtggtgtcgg catgcggatg gtcgtaaacg gcgttcagga ccgcgactct
     3061 cggccgggtc acccggagtt ccgagtcacg cagcaggctt tcgtattcgg cggcagagga
     3121 caccgcccat agttatatct cttttcttga atcagtcaag taaagacccg gtgaaccgga
     3181 ttcggaaata cgccggtagg tcgtctcgat cgactggtcc ttgcgcggac ccctgaccgt
     3241 ccatgacgtg cgaaacaggt tctccccgtc gatcgcgatg ctcccttcgt agagatcgtc
     3301 ccggcaggga tgtgtcactt cgggctcccc gtcgccgagc tcaagagggt ggaaggggcg
     3361 gccatcatcg aaatggaccc tccagcccga ggacaccgat ggatcgggcc tgatccacag
     3421 ttcgcggctg gcccgggtgt cggcttcccc gatctccagg cgggctgatt ccagccagcg
     3481 cagaccgcca ccctcgatcg tgaaggtggc ggtcccgtgc gctttgccgt ccaccatggc
     3541 gagccggtcg cggatctttc tctcgaaggt ccacttgccc ggaaagtagg tgaccggatc
     3601 aacggtcacg accggctcac cggtggactt cgctcatgtg gtgccttccg gtcctgcctg
     3661 ctggccgatg ctgaccggtt gcgactgacc gagcgacttg ccgttggcgt cgagggcttc
     3721 aaccgcgaag aacctcgcat ggctccggat cgtcttgccg gtctcgaagc cgtccttgtc
     3781 gacggtcgtc accggcttga ggttgtcctc atccggcccg gcgaggatcc gccatgaagc
     3841 ggtctcggtc gatccgttcc aggacatcca caccctgacc ttgttgtcac cgccgtcttc
     3901 cgtcgcgatg gccggatcat cggagggtat gccggtccac gggtcgcggt agacccggta
     3961 cgacgagttc cgggcgtagt aggtcccgtt gtagagaacc tttccttccg gggtgtactc
     4021 ggtgaaccag ggctgggcgc cccagccgac caggtagttg ccgttcggga gcttctgcag
     4081 gttgccctgg ctcggcgcga gcagcttctg cggactgatg aacggcttca gcgccagttc
     4141 cgccgtcatg ttctcctcgt ccaggtcaag gaccagtccc ctcgaggtcg ggtaggtcgg
     4201 ttcctggccg taggtgctgg cgttgtcgaa cacggtgagg gtgccgtcgt cctggcgcag
     4261 ggcgtcgtgc tggtaggaga agttggtccc cttgcccatc tcgaagtcgg acttcttgcc
     4321 gcccagcttc cagatggtct tgccggtcga cttgtccacc ttgagcacgc tgaacgtgtt
     4381 gcggccggaa atgatgatgt ttccgtcctt gtcctcgttg atcgagttga tgtggaagta
     4441 gtcgaacgct tcggtcggcg aggtcgggaa cggaatctcg gtctcgccca gtccgacatt
     4501 gcccaggctg tgccattccc agacgacctt gccactggcg atgtccactt cctgcgcgat
     4561 cgaatcgagc accgctccgc gcttgggtcc gccccagggg gtcaggtcca ccagcacggt
     4621 cttgtacgcc gtcaggtagg ccgaaccatg gtcggtcagc ttgaactcgt gaaggtccgc
     4681 ctggtagccg ttgccggcca gtacccggga gatctcctcg tacttgtcgt tggcgatgat
     4741 gttcgtgcct tcggtgtaac cgccggcatt gaaggggcct tcccaccagg tcaggaccgg
     4801 ctggccgttg taggtctggg tccggaagtc cgccgcttcg gtgctgggct tgtaatagac
     4861 cagcgcaccg tcatcgtcga agatcatcgg tccgtcgacc ttgggtgaca tgaacatcag
     4921 gccgtcgccg ctgcctgagg cagccggctt cttgatgtcg accaccggcg gttccagatc
     4981 cggccgcgag tggaaggtct catgcttgat gatccggctc tcgggcggaa gcaccttggg
     5041 cggcaacgcg gcatcggtgc ggaaggaagc cgtcttgaac ctgtattcgc ctcccgggtc
     5101 gaccccggcg atgtcaacgt ccccgtcgat gctgacggtg accgtttcct gagccttgaa
     5161 tggcttgtcg ggtgtgaaag tcaccccctc ggtcgacggt cccagtggtt tcagctgtcc
     5221 ctcgtggtct ccggacttcg agccggtgac cgtcacctgg gcgggatcct ccgagtccat
     5281 cccgacgagg gcgatcctcg tcacgggaga ggcggaaggc gtgcccgggc tggggtatgc
     5341 gcttatcgtt ttgctttctt cgtcagaccc tccacagccg gcggctgcga cggcgatcac
     5401 gagcagaagt cccgtgatca gacggggaag gcgaggcatg tttcctttcg ttggttgaag
     5461 acagactctt cacaggccgg tccgatggga tgcatcgcgg gtgccttgcc ggaccgtcct
     5521 gtccgggcta gcatcgttcg catggtcact ctgcccgcgg aggcgcgttc cctcatcgag
     5581 tcggatgcgc ttgcccatct ggtcacaatc aacccggacg ggagccccca ggtcacgtgc
     5641 atctgggtcg gcctggacgg agacgagatc gtcagcggtc acatgttcca cgtcctcaag
     5701 ctgaagaacg tagagcggga cccgcgcgtg gtgctctcga tcgaggcccg gacccgaaac
     5761 gagatgaacc tgcaggaata cctcgtcata cgggggaccg cccgggtcga agagggccgc
     5821 gcggccgagc tgcttcagga cctggcgcac acatatatgg gccccgacgc ggtctttccg
     5881 ccgcccgggg tggagagccc cggcttcgtc ctcagggtca ctcccgaacg cctcggcggg
     5941 gtcggcccct gggcctgatc ccgggctcgt ccgctgctcg tcgccgggcg aagttgacat
     6001 aacgtcccga aactggcttc cagactcaaa aatcctgcaa gttcgaacag tgtctggtaa
     6061 ggtcggtttc gtatgggggc atacgtcagg cacgagggtg gattcacggc tgcgcgaacg
     6121 cgggggagcc ttcg
//
LOCUS       DAOTIP010000020         6030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_15547_length_6030_cov_3.194477, whole genome shotgun sequence.
ACCESSION   DAOTIP010000020 DAOTIP010000000
VERSION     DAOTIP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15547_length_6030_cov_3.194477"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1811
                     /locus_tag="P5138_00795"
     CDS             195..1811
                     /locus_tag="P5138_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59145.1"
                     /translation="MTGTAFAAQGTWVQPSRVIAASDKGWQQDARVAVASDKTAIAVW
                     LVEHPNKEGSDDNTNSVMAAVRPPNGKFGKPFDLSGDAHGIWNLSVAAAGSDITVLWQ
                     RKDNYSVITRTRPAGGPFGPKVRLNDQGETWKPYEPELAGGADGTTAVSWRQPRSTPS
                     IIAQASIRPPGGAFGAPVDLMADQANATADSTAVAVADDGAVVVAWQQYGQGIQVAER
                     PSGGSFGAPIEPTGSESGYWPVVGVAPDGTTTVAWTGSSGGQGGIFATTQQPGGEFPA
                     ASRVARTEDPRESEMAVAGDGRITIVWPEWVTGGDPDYGYYHDTILKMVSWIPGQAKT
                     VKRLGWAGGWNFGGASVSGSADGSTTVVWPDRYGRDAGVQASAWTPGGEFSDPVRIST
                     DLPDMVNVATGPDGNAVAAWIAYRQSSVVVGTSSTVPAYCGRSTMSLGKVRRNPRNGT
                     AWMSVKLGSRGKVSTLRSPWIKPTTTRARKAGTYHVKVVPKGPARRLLKRTGWAEVKV
                     GFRFDPVAVPCQNAKAFEEIVLVRHNRRSR"
     gene            1811..3595
                     /locus_tag="P5138_00800"
     CDS             1811..3595
                     /locus_tag="P5138_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59146.1"
                     /translation="MTHLHAKAGPFTRVSFLAILLLPFLILTSVDANASPGPWLSPPK
                     PITPDNGEWVLSDPEVAVGPGRTATAAWLGFDPGSDGDSTNGRQIVRISTRPAWGRFA
                     APVKLVTTPVTSPSGGNLDEVQIAAGKGGTLVTWVGISGDHLVVQGSFRPRGGHFGKV
                     INLSDDPALRLTASAAGPDGTFAVAWVGVDDVVQVAVRKPGHGFSDPVDLSVAGPTVF
                     DLPDLAIDRNGTVSVIWSSADFANNRSELKVAKLPEGNWHGNRVTVARGTTDGTDPIE
                     DPRIAVAPDRSVSLAWRQGPPPVWDQDGAEVGRIYSATRKPGGHFTDPRPVSGASPVG
                     FDNSSPEIAIGGDGVVSIAFLKLLRFRGEDGDGNGIWLWYRVAYVATGNTHLGFHRRA
                     FGGGLEHSATSPGITASTDGSTTVLWSEKRASDPGLGSTLNAATRPPGGIYPRVDRWT
                     TIAYDWVDASSVASSPDGNVVAVWKKLDTEARSILTTSTIPAYCSKVRMKPGRLVRNR
                     DRGVARLAVRVTGRGRLSVVGSGLVRARSVNARRAGTYWLKITPTVPALHKLKRSGTL
                     RVKIDLKFDPRAKGCLKHSRTISVRLKY"
     gene            complement(3604..3765)
                     /locus_tag="P5138_00805"
     CDS             complement(3604..3765)
                     /locus_tag="P5138_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59147.1"
                     /translation="MSDPGGKNSGDSDHEMKRAPFRAWLVTGPVGRLVGFFLDFARAL
                     RAHRAAKRD"
     gene            complement(3758..5281)
                     /locus_tag="P5138_00810"
     CDS             complement(3758..5281)
                     /locus_tag="P5138_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRV59148.1"
                     /translation="MGAEIAGARLLAPYFGDSTIVWANTIAIVLVALSIGYWLGGKLA
                     DRRPDLNSLCATVLAGAILVTLVPFFARPFLGASVDAFDELSVGVFAGSLVGVLVLLA
                     IPVMIFGAVSPWALRIAMDSTDLEHAGQLAGRLYALSTAGSLIGTLASAMVTIPLIGT
                     QRTFILFALLVAVVASIGLRKVWPALVPAAVACAALVPVGTIKASSEGDVIYEAETPH
                     QYIRVVETDDGERRLELNEGQAVHSIYRPGEYLVGNYWDEHLVLPFVASPQVTGASPE
                     VTPSANSDPGPGNSPDWPGRIAILGNAAGTVARAYGRFYPDTEVDAVEIDGKVTEVGR
                     EYFGLNNPNMTVHTEDARPWLEAADPVYDVIMVDAYHQPYIPFYMATKEFFELVRDRL
                     RPGGVVLINVGHPENSDALEKNLTATLEEALPEVLRDPVTDTNTMLVGSATPIAARNI
                     LLATEGRKPPIPHRLDRLATDTAIRIRPPLTGGDIWTDDHAPVEWLVDLSLLDYANE"
     gene            complement(5325..>6030)
                     /locus_tag="P5138_00815"
     CDS             complement(5325..>6030)
                     /locus_tag="P5138_00815"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRV59149.1"
                     /translation="LVIAHMVEHIVIGDLAALFIVLGLTRSILQPILAIPFFNRLQVL
                     ANPFVAIPLWAINLFFWHIPVMYDAAYGGALMHGLEHSLFLFFGCLMWMPVFGPLPTP
                     KWFGPGWKVVYVVVVRFIAAILGNILMWTQVELYKNYDAGHLKWGITAVQDQSTAGVI
                     MMVEGTFLILGVLAWTFFEAAKQSTQKQDLMDLAFDRGVDIDEDRIELAVKSGHGDLL
                     EKRILAGDARVDAASR"
BASE COUNT         1098 a         1915 c         2035 g          982 t
ORIGIN      
        1 gcctagcgag gagcggttca atccgcgtcc cagtccctgt tctccggccc ccacggcacg
       61 gcccggggct ggttgcggga aaacaccggc ccggcgcgcg aggcgcaact cgaaatatct
      121 ggcagggttg aattggtgtg atcgtccgac ccagaattct gctcctcgcc ctgttcctaa
      181 tcgggcttac ttccgtgacc ggcacggcat tcgccgcaca gggcacctgg gtgcagccct
      241 caagggtgat tgcagccagc gacaagggct ggcagcaaga tgcgcgggtg gcggttgcct
      301 cagacaagac cgcgatcgcg gtgtggctgg ttgagcaccc gaacaaggag ggttctgacg
      361 acaacacgaa ctcggtgatg gcagccgttc gtccaccgaa cggaaagttc ggcaagccct
      421 tcgacctttc cggtgacgct cacgggatct ggaacctgag cgtcgccgcg gctggcagtg
      481 acatcacggt gctctggcag cggaaagaca actactcggt gatcacccgg acccggccgg
      541 ctggcggtcc tttcggcccc aaggtccggt tgaacgatca gggagaaacc tggaagccct
      601 acgagccgga actcgcgggg ggagccgacg gaacaaccgc ggtctcctgg cggcagccca
      661 ggtccacccc ttcgataatc gcccaggcat cgatcaggcc gccgggcgga gccttcgggg
      721 cacccgtgga cctgatggcc gatcaagcca acgcgacggc cgacagcaca gccgtcgcag
      781 tcgccgacga cggtgccgtg gttgtcgcct ggcaacagta cgggcaaggg atacaggttg
      841 ccgaacggcc gtccggcggc tcattcggtg cacccatcga accgaccggt tcggaatcgg
      901 ggtactggcc ggtggtcggc gtcgcgccgg acgggacgac gaccgtcgcc tggaccggtt
      961 ccagcggtgg ccagggcggg atcttcgcga caacgcagca gcccggcgga gaattccccg
     1021 ctgccagccg tgtcgcacgg accgaggatc cccgtgaatc cgaaatggcc gtggcgggcg
     1081 acggtcgcat cacgatcgtc tggcccgaat gggtaaccgg aggcgacccc gactacgggt
     1141 attaccacga cacgatcttg aagatggtca gctggattcc cggtcaggcg aagacggtca
     1201 aacgacttgg ctgggccggc ggctggaact tcggaggcgc atcggtctcc ggctcggccg
     1261 atggcagtac gacggttgtc tggcctgacc gatacggacg ggacgccggc gtacaggctt
     1321 cggcctggac tcccggagga gagttttcgg atccggtccg gatcagcacc gatctgccag
     1381 acatggtgaa tgtcgccacc ggccccgacg ggaacgcggt cgcggcctgg atcgcctaca
     1441 gacagagttc ggtcgtggtc ggtacctcgt ccaccgtgcc cgcctactgc ggcaggtcga
     1501 ccatgtccct gggcaaggtc aggcgcaacc cccggaacgg gaccgcctgg atgtccgtga
     1561 agctgggttc tcgcggcaag gtctcgacgc tccgttcgcc ctggataaag ccgacaacca
     1621 cccgagcccg taaggcaggc acctaccacg tgaaggtcgt gccgaaggga ccggcccggc
     1681 ggctcctgaa gcggacgggt tgggccgaag tcaaggtcgg attcaggttc gacccggtcg
     1741 ccgtgccctg ccagaacgcg aaggcattcg aggagatcgt gctggtcagg cacaaccgga
     1801 gaagccggtg atgactcatc tccacgccaa agccggcccg ttcacccggg tctccttcct
     1861 ggccatactg ctgctgccgt tcctgatcct gacgagcgtt gatgccaatg cttcccccgg
     1921 cccctggctt agccccccga agccgatcac gcccgataat ggcgagtggg tcctgtccga
     1981 tccggaggtc gcggtcggac cgggacgaac ggcgactgcc gcctggctcg gcttcgaccc
     2041 gggaagcgac ggggattcga ccaacggcag gcagatcgta aggatctcga cgcgtccggc
     2101 ctggggccgg ttcgctgcac cggtcaagct cgtaactacc cccgtaacga gcccgtcggg
     2161 gggaaatctg gatgaggtgc aaatcgcggc cggcaagggc ggcaccctgg tcacctgggt
     2221 cggtatctca ggcgatcatc tggtcgttca ggggagcttc cggcccaggg gcggccactt
     2281 cggaaaggtg atcaacctct ccgacgatcc ggctctccgg ctcacggcct cggcggctgg
     2341 ccctgacggc accttcgccg tcgcctgggt tggcgtagac gatgtggttc aggtggcagt
     2401 tcgaaagccc ggtcacggat tttccgatcc ggttgacctt tcggtggcag gacccacggt
     2461 tttcgacctt cccgatctcg cgatcgatcg caacggcacg gtgtcggtga tctggagcag
     2521 cgcggatttc gccaacaatc gctccgaact gaaggtggca aagctgcccg aaggaaactg
     2581 gcacggcaac cgggtcaccg ttgcccgcgg aacgactgac ggaaccgacc cgatcgagga
     2641 tccgcggatc gccgtcgccc ctgaccgctc ggtgtctctc gcctggagac agggtccccc
     2701 tcccgtctgg gaccaggacg gagccgaggt gggccggatc tattcggcga ccaggaagcc
     2761 cggcgggcac ttcaccgacc cccgacctgt ttccggcgcc agtcccgtcg ggttcgacaa
     2821 ttcttcaccg gaaatcgcaa tcggaggcga cggggtggtc tcgatcgcct tcctcaagtt
     2881 gctgagattc cgcggcgagg atggagatgg aaacgggatc tggctctggt accgggtggc
     2941 ctacgtggcg accgggaaca cccacctcgg gttccacaga cgggcattcg ggggcggtct
     3001 ggagcactcc gcaacttcac cggggatcac tgcctcgacc gacggctcga ccacggtcct
     3061 ctggtccgag aagcgcgcct cagatcccgg cctcgggtcc acgctgaacg cggcaacccg
     3121 ccccccagga ggcatctatc ccagggtcga ccggtggacc actatcgcct acgactgggt
     3181 ggacgcaagc tcggttgcct cgagtccgga tggcaacgtg gtcgccgtat ggaagaagct
     3241 ggacaccgaa gcccgttcga ttctcaccac ttcgacgatt cctgcctact gcagcaaagt
     3301 ccgcatgaaa ccgggcagac tggtccggaa ccgggatcga ggcgtggccc gtctggcggt
     3361 tcgggtcacc ggacggggca ggctgtcggt ggtgggaagc gggttggtca gggcccggtc
     3421 cgtgaacgca cggcgggccg gcacctactg gctgaagatc acaccgaccg tgccggcgct
     3481 gcacaaactg aagcgaagcg gaaccttgcg ggtcaagata gatctgaagt ttgacccgcg
     3541 agccaagggc tgcctgaagc attcgcgaac gatctcggtc cggctgaagt actgacggcg
     3601 ggatcagtcc cgtttggcgg cgcgatgggc cctgagggcc cgggcgaagt cgaggaagaa
     3661 gccgacgaga cggccgaccg gaccggtgac cagccaggcc ctgaaagggg cgcgcttcat
     3721 ctcgtgatcg gagtcgccgg agttcttgcc gccgggatca ctcattggcg tagtccagca
     3781 gggaaaggtc gaccagccat tcgaccgggg cgtggtcgtc ggtccagatg tctccgccgg
     3841 tcaggggcgg acggatccgg atcgccgtgt cggtggcgag gcggtcgagt cggtggggaa
     3901 tcggcggctt tctcccctcg gtggccagca ggatgttacg ggccgctatc ggagtggccg
     3961 atccgaccag catcgtgttg gtatccgtga ccgggtcgcg gagaacctcg ggcagggctt
     4021 cctcgagcgt ggccgtcagg ttcttttcga gcgcgtcgct gttttcggga tggccgacgt
     4081 tgatcagcac caccccgccc ggcctgagcc ggtcccggac cagctcgaag aactccttcg
     4141 tcgccatgta gaacgggatg tacggctgat ggtaggcgtc gaccatgatc acgtcgtaga
     4201 cgggatccgc cgcctcgagc cagggcctgg cgtcctcggt gtggaccgtc atgttcggat
     4261 tgttgaggcc gaaatactcc ctccccacct cggtcacctt gccgtcgatc tcgaccgcat
     4321 ccacctctgt gtccgggtag aagcgaccgt aggcccgggc gactgtgccc gccgcgttgc
     4381 cgagaattgc gatccggccc ggccagtcag gcgaattccc gggacccggg tcgctgttcg
     4441 ctgagggcgt gacttcaggt gaagcgccgg tgacctgggg cgacgcaacg aagggcagta
     4501 ccaggtgctc gtcccagtag ttgccgacca gatattcgcc gggacggtag atcgaatgaa
     4561 cggcctggcc ctcgttcagt tccagacgac gttccccgtc gtcggtttcg accacccgga
     4621 tgtactggtg cggggtctcg gcctcgtaga tcacatcgcc ttcgctggaa gccttgatcg
     4681 tgccgaccgg gaccagggcg gcgcaggcga cggcggccgg gacgagcgca ggccagacct
     4741 tgcgcagccc gatcgaggcg accaccgcga ccagcagggc gaagaggatg aaggtccgct
     4801 gggtgccgat cagggggatg gtcaccatcg ccgaagcgag cgttccgatc agggacccgg
     4861 cggtcgaaag cgcgtagagg cgaccggcca gctggccggc atgctcgagg tcggtggaat
     4921 ccatcgcgat cctcagcgcc cagggggata cggcgccgaa gatcatcacc gggatcgcca
     4981 gcagcacgag caccccgacc agggagccgg cgaacacccc gaccgaaagc tcgtcgaagg
     5041 catcgaccga ggcgcccagg aatggccggg cgaaaaacgg gaccagggtg acgagaatcg
     5101 ccccggcgag gacggtggca cagagggaat tcaggtcggg acggcggtcg gccagcttgc
     5161 cgccgagcca gtacccgatc gagagggcga ccaggacgat cgcgatcgtg ttcgcccaga
     5221 cgatggtcga atcgccgaag tacggggcga gcagcctggc ccccgcgatt tcggcgccca
     5281 tcgtgcaggc gcctgtggtg aaaacaagca ggccgagaaa ccggctaccg gctggccgca
     5341 tctacccttg cgtccccggc caggatcctc ttttccagca gatccccgtg gccggacttg
     5401 accgcgagtt cgatccggtc ctcgtcgatg tccactccgc ggtcgaaggc gaggtccatc
     5461 aggtcctgct tctgggtgga ttgcttcgcc gcctcgaaga aggtccatgc caggacaccg
     5521 aggatcagga acgttccctc caccatcatg atcaccccgg cggtgctctg gtcctggacc
     5581 gctgtgattc cccacttcag atggccggcg tcgtagttct tgtagagctc gacctgggtc
     5641 cacatcagga tgttgccgag gatcgcggcg atgaagcgca cgacgaccac gtagacgacc
     5701 ttccagcccg ggccgaacca tttcggggtc ggcagggggc cgaataccgg catccacatc
     5761 aggcagccga agaacaggaa gaggctgtgc tccaggccat gcatcaacgc tcccccgtat
     5821 gccgcgtcat acatgaccgg gatgtgccag aagaagagat ttatcgccca gagcggaatc
     5881 gccacgaagg gattcgcgag tacctgcagg cgattgaaga aggggatcgc gaggatcggc
     5941 tgcaggatcg aacgggtcag gccgagcacg atgaagaggg ccgccagatc accgatcacg
     6001 atgtgttcga ccatgtgggc gatgaccagc
//
LOCUS       DAOTIP010000021         5968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_15793_length_5968_cov_3.302723, whole genome shotgun sequence.
ACCESSION   DAOTIP010000021 DAOTIP010000000
VERSION     DAOTIP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15793_length_5968_cov_3.302723"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1032)
                     /locus_tag="P5138_00820"
     CDS             complement(232..1032)
                     /locus_tag="P5138_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRV59150.1"
                     /translation="MKLLFIGDVVGSPGRKGLAAVMPRLREEHMPDLIVVNGENSAGG
                     LGITEKTADEIFACGADVITLGNHSYRQRESWEYLDRAERVVRPANYRRANPGKGHCV
                     VTVNPADGSPGRRVAVINLIGKINLDAARSPFDEADLILADLEKNGTADTVIVDFHAE
                     VTSEKVAMGWYLDGRVAAVLGTHTHVPTADGRVLPQGTAFISDVGMTGARESVLGVKK
                     EQAIEKMRTDMPVRFETATEDVWVMGAAIEIDENGLATGFTQVLEPVS"
     gene            1173..1928
                     /locus_tag="P5138_00825"
     CDS             1173..1928
                     /locus_tag="P5138_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV59151.1"
                     /translation="MPPQVTGSNPALAARGVSKSFDGRKALSGVDFEVGPGEVVALIG
                     PNGAGKTTLLSILAGIAEPDSGEVTGDGVAWVPQQAALYRRLSVRENLLLFAHLQHDA
                     GQTAENPEQMVTRMLELTGLADRAGDRVGTLSGGNQQRVNIAIGLMADPSVLLLDEPS
                     VGLDPAQRERLWGFVLGLVGEGTAVAFSTHDIQEAERYAERIVVVAEGEGIFSGTADE
                     LRQAVEAGAAGGGPAAAGDLETSFLAFLRERGH"
     gene            1928..3271
                     /locus_tag="P5138_00830"
     CDS             1928..3271
                     /locus_tag="P5138_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV59152.1"
                     /translation="MRWLLLKDLQILKRSPLQLTLLILYPIVIALLVGFAMTREQDQT
                     KVALYNAMPAGEPLGFGGEEVDGNEIRDQLCGRVDCIEADSTADARKMVENGEATAAV
                     IVPEDLGDKIRSLASLSPESPEIQIIVNGSDAVENQVTDDRIEAMLGEANLLLAKRLS
                     EGAKQYLDLILKGGEFDLLGQQIEVLGLQKSEQILKKIEPKLPPGTDKEQLQRAVRFA
                     HLATDNLDLAGPLLTAITEPIKADKVEVGDEAPSLDTFAIGVTVAFALMFVTVLLVAG
                     SLALEKEENAFTRLTAGLVGRGQILVAKVGLGTVVGTGVALILLVGLGLFVGIGWGRA
                     PLWLLALIFGAAAFSAGGAALGAAAREVRAATLAAVMVCLPVALLSLVPDDAVGPAIE
                     TAINLVSAVFPFRPTLDAITGGLEISGPSLWLPLLHLLLLTATYGALARLALRRF"
     gene            3395..4366
                     /gene="hemB"
                     /locus_tag="P5138_00835"
     CDS             3395..4366
                     /gene="hemB"
                     /locus_tag="P5138_00835"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRV59153.1"
                     /translation="MSFPSTRLRRLRRTPALRGLVRETSLSPADLIQPLFVTAGTDLR
                     EPVESMPGVNRFSITALVEEAGEIEAAGIPAVLLFGIPSEKDEAGSGAYDDEGVVQMA
                     VRALKEAHPDLVVITDVCLCEYTSHGQCGFVRDGQVDNDVTVELLARTAISHAEAGAD
                     IVAPSDMMDGRIGSIRYQLDEEGHPDTAILSYAAKYASAFYGPFREAADSTPQIGDRL
                     GYQLDPSNSDEAIREAKLDLEEGADALMVKPASHYLDIVRRVKDETGAPVAAYQVSGE
                     YSAIKAAGANGWIDEKAAALEALVSMRRAGADFVVTYFAKEAAGWLD"
     gene            4354..5781
                     /gene="hemL"
                     /locus_tag="P5138_00840"
     CDS             4354..5781
                     /gene="hemL"
                     /locus_tag="P5138_00840"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRV59154.1"
                     /translation="MAGLSRGAPDKVTIKVRGDGRPLGETNQEVEYQEAKAKQLEEAR
                     GAENRLDNSELFERARRVLPGGVNSPVRAMRQVGLDPVFIESAEGAEVIDRDGRRYLD
                     WVGSWGPMILGHADSEVLTAIEDAASRGTSFGAATELEVELGEEVASRMPSVEMIRMT
                     SSGTEAAMTAARLARAATGREILVKFAGAYHGHSDGLLAEAGSGVATHGIPSGPGIPA
                     AQAAGTVVVPWNDREALAEALQKHEVAAVFAEPIAANMGVVPPAEGFLDFLGEATEQS
                     GSLLVFDEVITGFRVARGGAQELYGVNPDLTVMGKIIGGGLPAAAVGGSRELMERLAP
                     AGDVYQAGTLSGNPLAVAAGLATLRRLDDDAYARLGALTDRLASGLADLADQHGLTVA
                     SAPGLVTLFFSALPVKNFDDARATDGERHAAFFRAMLERGIWLPPSRFEAWFVSLAQD
                     EIQVDRTLMAAAEALSATDPGRSVG"
     gene            5778..>5968
                     /locus_tag="P5138_00845"
     CDS             5778..>5968
                     /locus_tag="P5138_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59155.1"
                     /translation="MSAATGSEALERLAARVREEGPPLTVKESPARPMAGDPRYGELA
                     ATGPRTRGKAGEYAFVVEAV"
BASE COUNT          975 a         1980 c         2011 g         1002 t
ORIGIN      
        1 aaaccgccgc ccgtcatcgg gcccggccgc agtggggact acaggacgtc gccccgatca
       61 tccgcgagca tttcgcagtt tcgtaacaaa gtccctgcaa acagccgtca ctctcgtgtc
      121 aaatgaacag gacacggaga accacgagaa gagtcgaatt cctcgtcacc tcggctgcag
      181 tactggttgc ctacgcgatc aactatctcg atccgatccg gatctgagta gtcaggaaac
      241 aggttcgagg acctgggtga agccggttgc gagaccgttc tcatcgatct cgatggccgc
      301 gcccatcacc cagacatctt ctgtggcggt ctcgaagcgc accggcatgt cggttcgcat
      361 cttctcgatc gcctgctcct tcttcactcc gaggaccgat tcacgggcgc cggtcatgcc
      421 aacgtccgag atgaatgcgg ttccctgcgg cagcacccgg ccgtcggcgg tcggcacatg
      481 cgtgtgggtt ccgagcaccg ccgcgacccg accgtccagg taccagccca tcgccacctt
      541 ctcgctggtc acctcggcgt gaaagtcgac gatgaccgtg tcggccgtac cgttcttctc
      601 caggtcggcc aggatcaggt cggcttcgtc gaatggcgag cgggccgcat ccaggttgat
      661 cttgccgatc aggttgatca ctgcgacccg ccgacccggt gaaccgtcgg cggggttcac
      721 ggtcaccacg cagtgtccct tgccgggatt ggcccgccgg tagttggccg gccggaccac
      781 cctctcggcc cggtccaggt actcccagct ctcgcgctgc ctgtagctgt ggttgccgag
      841 ggtgatcacg tcagccccgc aggcgaagat ctcgtcggcc gtcttttcgg tgatgccgag
      901 gccgccggcc gagttctcgc cgttgaccac gatcaggtcc ggcatgtgtt cctcacgcag
      961 tcgcggcatt acggcagcca ggcccttccg gcccggactg ccaaccacat cgccaatgaa
     1021 gagcagcttc atgcgccaag tttaggtgcg ctggtgctgc cgttgagcgg agcccctttc
     1081 ctccatcagt tgacgtgagg ggaaggggcg ggtttccgtg gtcatccggc gaagaccgga
     1141 tgacatgtgg atgtttgggt aggttctccg aggtgccccc tcaggtgaca ggttccaacc
     1201 ccgcgctggc cgcccgtggg gtctccaagt catttgacgg ccgcaaggcc ctgagcggcg
     1261 tcgacttcga ggtcggtccg ggcgaggtcg ttgccctgat cgggccgaac ggggcgggta
     1321 agaccaccct gctttcgatc ctcgccggga tcgccgaacc cgattccggg gaagtgaccg
     1381 gcgacggcgt cgcctgggtt ccgcagcagg cggctctcta ccggcgactc agcgtgaggg
     1441 agaacctcct gctcttcgct cacctccagc atgacgccgg gcagaccgcc gagaaccctg
     1501 aacagatggt cacgcggatg cttgagttga ccggtctggc cgaccgggcc ggtgaccggg
     1561 tcggcaccct ctccggcggc aaccagcaaa gggtcaatat cgcgatcggg ctgatggccg
     1621 acccttcggt tctgttgctg gacgaaccga gcgtcggtct cgatccggcc cagcgcgaaa
     1681 ggctctgggg ctttgtgctc ggcctggtcg gcgagggaac ggcggtcgcc ttctccaccc
     1741 atgacatcca ggaggccgag cgttacgccg agcggatcgt ggtggtggcc gagggcgagg
     1801 ggatcttcag cggcaccgcc gacgaactcc gccaggcggt cgaagccgga gccgcgggcg
     1861 gcggtcccgc cgcagccggc gacctcgaga cgtccttcct cgccttcctc agggagcggg
     1921 gccactgatg cgctggctgc tgctcaagga cctgcagatc ctcaagcgct cgccgctgca
     1981 gctcaccctg ctgatcctct acccgatcgt gatcgcactc ctggtcggct tcgcgatgac
     2041 cagggagcag gatcagacca aggtcgcgct ctacaacgcg atgccggccg gggagccact
     2101 cggcttcggt ggggaggagg tcgacggcaa cgagatccgc gaccagcttt gcggccgggt
     2161 cgactgcatc gaagccgaca gcaccgccga cgccaggaaa atggtggaga acggagaggc
     2221 cacagcggca gtgatcgtgc cggaggatct gggggacaag atccgctcgc ttgcttcgct
     2281 cagcccggag tcgccggaga tccagatcat cgtcaacggg tccgacgcgg tcgagaacca
     2341 ggtcaccgat gaccggatcg aggcgatgct cggcgaggca aacctcctgc tggccaagcg
     2401 cctctccgaa ggcgcgaagc agtacctcga cctgatcctc aaggggggcg agttcgattt
     2461 gctcggccag cagatcgagg tgctcggcct gcagaagagc gaacagatcc tgaagaagat
     2521 tgagccgaag ctgccaccgg gaaccgacaa ggagcaactg cagcgggcgg tgcgcttcgc
     2581 ccatctcgcg accgacaacc tggacctggc cggtcccctg ctgaccgcga tcaccgaacc
     2641 gatcaaggcc gacaaggtcg aggtcgggga cgaagcccct tcgctcgaca ccttcgcgat
     2701 aggggtgacc gtcgccttcg ccctgatgtt cgtgaccgtg ttgctggtcg cggggtcgct
     2761 ggcactggag aaggaagaga acgctttcac acgactcacg gccggcctgg tcggccgggg
     2821 ccagatcctc gtcgccaagg tcggccttgg aacggtggtc ggcaccggag tggcgctgat
     2881 ccttctcgtg ggtctgggac ttttcgtcgg gatcgggtgg gggagagccc ccctctggct
     2941 gctggcgctg atcttcggcg cggccgcttt ctcggccggt ggcgctgccc tcggggccgc
     3001 ggcgcgcgag gtacgggcgg caactctcgc cgcggtgatg gtctgcctgc cagtcgccct
     3061 gctgtcgctg gtcccggatg acgcggtcgg cccggcgatc gagaccgcca tcaacctggt
     3121 cagcgcggtc ttccccttcc gaccgaccct cgacgcaata accggaggcc tcgaaatctc
     3181 aggcccctcc ctctggctac ccctcctcca cctattgctc ttgacagcga cctacggggc
     3241 cctcgccaga ctcgccctga ggcgtttcta agcgagctcg gtttggtgtg gtgcgtgggg
     3301 tgccgaaaaa agatggatag tgagcgcagc gatatctttt ttcggtaccc cacgtgccgc
     3361 accccaccac gaacgccccg cctagactga agttatgagt tttccctcca ctcgccttcg
     3421 gcgtttgcgg cgaaccccgg ccctgcgggg actggtccgt gaaacgagtt tgagcccggc
     3481 cgatctgatc cagccgctgt tcgtcaccgc cggcaccgac ctgcgcgaac cggtcgagtc
     3541 gatgcccggg gtgaaccggt tctcgatcac cgcgctggtc gaggaagccg gcgagatcga
     3601 agcggccggc atcccggcgg tgctgctgtt cgggattccc tccgaaaagg acgaggccgg
     3661 cagtggcgcc tatgacgacg aaggtgtcgt ccagatggcg gtgcgggccc tgaaggaggc
     3721 ccatcccgac ctggtcgtga tcaccgacgt ctgcctctgc gaatacacct cgcatggcca
     3781 gtgcggcttc gtccgggatg gtcaggtaga caacgacgtg acggtcgagc tgctggcccg
     3841 cacggcgatc tcccatgccg aggccggggc cgacatcgtc gccccgagcg acatgatgga
     3901 cggccggatc gggtcgatcc gctaccagct cgacgaggag ggccatcccg acacggcgat
     3961 cctctcctac gcagccaagt acgcctccgc cttctacggg ccgttccgcg aggcagccga
     4021 ttcgaccccg cagatcggcg accgcctcgg ctaccagctc gacccctcca actccgacga
     4081 ggcgatacgc gaagcgaagc ttgatctcga ggagggggcc gatgcgctga tggtgaagcc
     4141 ggcctcccac tacctcgaca tcgtccggcg ggtcaaggac gagaccggcg ccccggttgc
     4201 cgcctaccag gtctccgggg agtattcggc gatcaaggcg gccggtgcga acggctggat
     4261 cgacgagaag gcggcggcgc tcgaagcgct cgtctcgatg cgccgggccg gagcggattt
     4321 cgtggtcacc tacttcgcaa aggaggctgc cggatggctg gactgagccg gggcgcaccg
     4381 gacaaggtca cgatcaaggt ccgcggagac ggtcgtccgc ttggcgagac caatcaggag
     4441 gtcgagtacc aggaggccaa ggccaagcaa cttgaggaag cgcgcggtgc cgagaatcgc
     4501 ctcgacaact cggaactctt cgaaagggcc cgtcgcgtgt tgccgggagg cgtcaactct
     4561 ccggtccggg cgatgcggca ggtcgggctc gacccggtct tcatcgaatc cgccgaaggg
     4621 gccgaggtaa tagaccgcga tggccgccgt tatctggact gggtcggatc ctgggggccg
     4681 atgatcctcg gccacgccga ctccgaggtt ctgacggcta tcgaggacgc ggcctcccgc
     4741 gggaccagct ttggcgctgc gaccgaactc gaggtcgaac tgggcgagga agtggcctcg
     4801 cgcatgccat cggtcgaaat gatccgcatg accagttcgg gaacggaggc ggcgatgacc
     4861 gcggctcgac tggcccgggc ggcgaccggc cgggagatcc tggtcaagtt cgccggtgcc
     4921 taccacgggc attccgacgg gctgctggcc gaggccggtt ccggcgtcgc gacccacggc
     4981 atcccctccg ggcccggcat ccccgccgcc caggcggccg gcacggtggt ggtgccctgg
     5041 aacgaccgcg aagcgctggc agaggctctg caaaagcacg aggtagccgc agtcttcgcc
     5101 gagccgatcg cggccaacat gggcgtggtg ccaccggccg agggattcct cgatttcctg
     5161 ggtgaagcga ctgaacagtc tggatctttg ctggtcttcg acgaggtgat caccggcttt
     5221 cgggtcgcca ggggcggcgc ccaggagctc tatggggtga accccgacct gacggtgatg
     5281 ggcaagatca tcggaggtgg cctgcctgct gcggccgtcg ggggcagccg cgagctgatg
     5341 gaaagactgg ctcccgccgg tgacgtctac caggccggga ccctttcggg gaatccgctg
     5401 gccgtcgcgg ccggactggc gactctgcgc cggctggacg acgacgccta cgcccggctc
     5461 ggcgccctca ccgaccgcct ggcctccgga ctggccgacc tggccgatca gcacgggctg
     5521 acggtcgcat cggcccccgg actggtcacc cttttcttct cggccctgcc ggtgaagaac
     5581 tttgatgacg ccagggcaac cgacggggag cgccacgcgg ccttcttccg ggccatgctc
     5641 gaacggggca tctggctgcc gccttcccgg ttcgaggcct ggttcgtctc gctcgcccag
     5701 gacgagatcc aggtcgaccg gaccctgatg gccgctgcgg aggcgctgtc tgccacagac
     5761 cccggcaggt cggtcgggtg agcgcggcga ctggttcgga ggcgctggaa cgtctcgccg
     5821 cccgggtgcg tgaagaaggt ccgccgctca ccgtgaagga gtcgcccgcc cgcccgatgg
     5881 ccggggaccc ccgctacggg gaactggccg cgaccggccc ccggaccagg ggcaaggccg
     5941 gcgagtacgc cttcgtggtc gaggcggt
//
LOCUS       DAOTIP010000022         5952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_15861_length_5952_cov_3.187892, whole genome shotgun sequence.
ACCESSION   DAOTIP010000022 DAOTIP010000000
VERSION     DAOTIP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15861_length_5952_cov_3.187892"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /gene="ftsH"
                     /locus_tag="P5138_00850"
     CDS             complement(<1..1369)
                     /gene="ftsH"
                     /locus_tag="P5138_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRV59156.1"
                     /translation="MKRFFKSAAFPILLVVILAFVAQRVISPDSGDKPPTYNEFLTMV
                     DKGEIDSVTVNTKNNSLDVTEKAADGSDSEGTKFETGYPPNTEQSLINQLREGDVATT
                     VKGTGGSSLLSMLTYILPFLLFFGFWIFLMNQMQGGGSRVMSFGKSKAKKMAVDAPKI
                     TFKDVAGADEAVQELQEIKEFLQTPKKFQSLGARIPKGVLLYGPPGTGKTLLARAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRHRGAGMG
                     GGHDEREQTLNQLLVEMDGFEIKDNIILIAATNRPDILDPALLRPGRFDRQIPVGQPD
                     LKGRQAILAVHAKGKPLAKDADLKALARQTPGFTGADLSNLINEAALLTARSGKREIT
                     QNELEEGIMRVIAGPEKKTRIMSEKERKITAYHEMGHAIVGHLLPNCDPVHKVSVISR
                     GQALGY"
     gene            complement(1499..2062)
                     /gene="hpt"
                     /locus_tag="P5138_00855"
     CDS             complement(1499..2062)
                     /gene="hpt"
                     /locus_tag="P5138_00855"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRV59157.1"
                     /translation="MAATPPNQLVSVGEVLISAEELQQKVVELGAQISRDYAGKDLVM
                     VGVLRGAVVFIADLLRHLSIPCEIDFMAVSSYGSSRDSSGVVRILKDLDDSITGRDVL
                     IVEDIVDSGLTLNYLIRNLQARDPASLEVCALLTKPERRRVDVPIRYTGFEIPNAFVV
                     GYGLDFAQRYRNLDHVALLPEDAVPDR"
     gene            complement(2073..3488)
                     /gene="tilS"
                     /locus_tag="P5138_00860"
     CDS             complement(2073..3488)
                     /gene="tilS"
                     /locus_tag="P5138_00860"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRV59158.1"
                     /translation="MLDPRFDPKRFFFVLEPIRASGLVERGDGGVVMVSGGADSVCML
                     GGLTTVAGSEGLVALHVNYGLRDEADRDEQLVRELCSGLGVELEVVRAGEPEGNVQGW
                     ARDIRHREAERLRAERGLDWIAVGHNRTDQVETFLYRLASSPGTRPLLVMPPRSGRLI
                     RPLLALDREFIRSEAERTEVRFVNDATNDDLSYARNRIRHEVIPALEQVNPAARQNIL
                     RTRDEMEEDEDLIGDLAGQAIADGIGEGAEFPAGLLLGLHPAVQRRVLRRMAEGALGR
                     PVAIPPGLAAEAVRLAGHPEGGVLDLGGGDVLSVESGLIGVNSIAESTDTDPPAPVTI
                     PARGGKVRFGGWSIEAELIDGKTAMAMFGDPFQTFFGSEFQEWILAEIGDIPAVRAPE
                     VRVWGPGDRVNPIGLGGSRSLQDVFTDAKVPRSKRRAWPVVWFGDRLLWVPGLVRSTH
                     CLVTSESERVLRLEAKPPFAI"
     gene            complement(3475..4662)
                     /locus_tag="P5138_00865"
     CDS             complement(3475..4662)
                     /locus_tag="P5138_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HRV59159.1"
                     /translation="MPGDDRISLNEAARRAGVSPATLTRWADEKVVPVRKGRWTPAAA
                     AQARIVARMRERGHSLEELKEAGKEGKLSFGMAEDLFADKSERVDLATAAEETGLSVE
                     VIERAMTVMGTPADREMTLSARDIEAFRRMGEVVEAGLPVEAMLQLTRVYAQAVRRIA
                     DAEVRLFHLFVHEPMIRDGLSEIEMAEELGDLARRTSPTAVPLFEYLHDRYLRYFLEQ
                     DVIGHMESDFGGDTNLDQVRTTLCFIDLTGFTRFTEQEGDETAFSVVEQFTETVEATL
                     PPEAGIVKTIGDEVMVVSPDPGSLAEWAVGFLALFADRPKPRAGIHHGAVVFRDGDYY
                     GSQVNLAHRVVNRALGGEVLITDAVAAALEGHPDLETDPIGEVELKGFPVPTPLFLIR
                     ARS"
     gene            complement(4681..5760)
                     /locus_tag="P5138_00870"
     CDS             complement(4681..5760)
                     /locus_tag="P5138_00870"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRV59160.1"
                     /translation="MADRHRIGVLVTGGTVGSRSEPLSGREKLVRLSEAGDQLDILAG
                     PHVSAVLEKGGIETILRQPLNMLSENMTPDHWVAVGREIKRLIAKEDPEGILVLHGTD
                     TMSFTSAALSFMLAGIKVPVVLTGSNRPPNEPDTDADTNIADAMIALQHLSPGVYLSF
                     AGRPNAKSRVHDGVSVRKARGSGPAFISAGRVHVAEVSWGSFEWTLKNRSATGTPQDG
                     PDSFRIAADDRVMRIDLHPGINLKALADTLICEGYRGAVMTLYPALTGPFEPEKSSVP
                     RFAETCAEHEIVLALTMNAQPVGVQNAYESKLLLEETGAVLLPVIPELALVKLMWALG
                     WSEDYSQVREILGAEVCNEFGGKPI"
BASE COUNT         1001 a         1950 c         1926 g         1075 t
ORIGIN      
        1 tgtacccgag ggcctggcca cggctgatca cggacacctt gtggaccgga tcgcaattcg
       61 ggagcaggtg gccgacgatc gcgtgaccca tctcgtggta cgcggtgatc ttccgttcct
      121 tctcggacat gatccgggtc ttcttctccg gaccggcgat cacccgcatg atcccttcct
      181 caagctcgtt ctgggtgatc tcgcgcttgc ccgaacgggc ggtcagcagg gcggcctcgt
      241 tgatcaggtt ggaaaggtcg gcaccggtga atcccggggt ctggcgggcc agcgccttga
      301 ggtcggcgtc cttagccagc ggcttgccct tggcgtgcac ggcaaggatc gcctgccggc
      361 ccttcaggtc gggctggccg accgggatct gccggtcgaa gcggcccggg cgcagcagcg
      421 ccgggtcgag gatgtcgggc cggttggtgg cggcgatcag gatgatgttg tccttgatct
      481 cgaagccgtc catctcgacc aggagctggt tgagggtctg ctcgcgctcg tcgtggccgc
      541 cgcccatgcc ggcgccgcga tggcggccga ctgcgtcgat ctcgtccatg aagatgatgc
      601 agggcgagtt ctgcttggcc tgctcgaaga ggtcgcggac ccggctggca ccgacgccga
      661 cgaacatctc gacaaagtcg gagcctgaaa tcgagaagaa cggcaccccg gcctcgccag
      721 ccacggcgcg ggcgagcagg gtcttgccgg tgccgggcgg cccgtaaagc agcacgccct
      781 tggggatacg ggcaccgagc gactggaatt tcttcggggt ctggaggaac tccttgatct
      841 cctggagctc ctgcacggcc tcgtcggcgc cggccacgtc cttgaaggtg atcttggggg
      901 cgtcgacggc catcttcttc gccttcgact tgccgaagct catgacccgc gagccgccgc
      961 cctgcatctg gttcatcagg aagatccaga agccgaagaa gagcaggaag ggcaggatgt
     1021 aggtgagcat cgaaagcagg ctgctgccgc cggtgccctt gaccgtggtc gcgacatcac
     1081 cttcgcggag ctggttgatc aggctctgct cggtgttggg cgggtagccg gtctcgaact
     1141 ttgtgccttc ggaatccgat ccgtccgccg ccttctcggt gacgtcgagg gagttgttct
     1201 tcgtgttgac cgtgaccgag tcgatctcgc ccttgtcgac catggtcagg aattcgttgt
     1261 aggtcggcgg cttatcgccg ctgtccgggc tgatcacgcg ctgcgcgacg aaggccagga
     1321 tcaccaccag caggatcggg aatgccgcgc tcttgaaaaa ccgtttcaaa tctcgggtct
     1381 ctttccacgg cccatggcaa gctttccctg cctttgggcc gaattgtcag actagcaggg
     1441 agaacccgcc aaccggcatg ggagagcggt gccgaacgaa gatttctcga atccggttct
     1501 agcggtcggg aaccgcgtct tcggggagca gcgccacatg gtcgaggttg cggtaacgct
     1561 gggcgaagtc cagcccgtaa ccgaccacga aggcattcgg tatttcgaag ccggtgtagc
     1621 ggatcggaac gtcgacccgg cgacgctccg gcttggtcag cagagcacag acctcgagcg
     1681 aggccggatc gcgggcctgg aggttccgga tcaggtagtt gagggtcagt cccgagtcga
     1741 cgatgtcctc gacgatgagt acgtctcgac cggtgatcga atcatccagg tccttcagaa
     1801 tccgcaccac gcctgaagaa tcccgggagg atccgtagct ggagacggcc atgaagtcga
     1861 tttcgcaggg gattgacagg tgccggagca ggtcggcgat gaaaaccacc gcgccccgca
     1921 ggacaccgac catcaccagg tccttccccg cgtagtcgcg gctgatctgg gcgccgagtt
     1981 ccacgacctt ctgctgaagc tcctcggcgg agatcaggac ctcaccgacg gagacaagtt
     2041 gattcggagg cgtcgcagcc atcgggctca gtctagatgg cgaagggcgg cttggcttcg
     2101 aggcggagaa cccgctccga ttcgctggtc acgaggcagt gggtggaccg gaccaggccc
     2161 ggaacccaga gcagccgatc cccgaaccag accaccggcc aggcccggcg cttcgatcgg
     2221 ggaaccttgg cgtcggtgaa gacgtcctgg aggctgcggc tacccccgag tccgatcgga
     2281 ttcacccggt caccgggtcc ccagactcgt acttccggag cccgaaccgc gggaatatcc
     2341 ccgatctcgg ccagaatcca ctcctgaaac tcgcttccga agaaggtctg gaacggatcg
     2401 ccgaacatgg ccatggccgt cttcccgtcg atcagttccg cctcgatgct ccagccgccg
     2461 aagcggactt tcccgccccg ggcggggatc gtcaccggtg ccggcgggtc cgtatccgtt
     2521 gattcggcga tcgaattcac cccgatcagg ccggattcga ccgacaggac atctccaccg
     2581 cccagatcga gcaccccgcc ttcggggtgg ccggccagtc gcacggcctc ggcggccagg
     2641 ccagggggaa tggctaccgg cctgcccagc gcgccttcgg ccattcgcct cagaacccgc
     2701 cgctggactg cggggtgcag cccgagcagg agaccggccg gaaattcggc cccttccccg
     2761 atcccgtccg cgatggcctg cccggccaag tccccgatca gatcctcgtc ttcctccatc
     2821 tcgtcacgcg tccggagaat gttctgcctc gctgccggat tgacctgttc gagcgcgggg
     2881 atcacctcgt ggcggatccg gttgcgggca taggaaagat cgtcgttggt cgcgtcattg
     2941 acgaatcgca cctccgtcct ttcggcttcc gaccggatga actcacgatc cagggcaagc
     3001 agcggacgga tcaggcgtcc ggatctcggc ggcatcacga gcagcggcct ggttccgggg
     3061 gacgatgcga gacggtagag aaaggtctcg acctggtcgg tcctgttgtg gcccaccgca
     3121 atccagtcga ggccgcgttc ggcgcgaagc cgctcggctt cccggtgacg gatgtcccgg
     3181 gcccatccct ggacgtttcc ttccggctct ccggcccgga ccacctccag ctcgactccc
     3241 aggccggaac agagttcgcg gaccagctgc tcgtcccggt cggcttcgtc tctcaggccg
     3301 tagttgacat gcagggccac gaggccctct gacccggcaa cagtggtgag ccctccgagc
     3361 atgcagaccg aatccgcgcc gccggaaacc atgaccacgc cgccgtcgcc ccgttcgacc
     3421 agaccggagg cccggatcgg ctccagaacg aagaagaatc gcttgggatc aaacctagga
     3481 tcgagcacga atcaggaaaa gcggtgtggg gacggggaat cccttcagct cgacttcgcc
     3541 gatcgggtct gtttccagat ccggatggcc ctcgagcgcc gccgcgacag catcggtgat
     3601 cagcacctct ccgcccagcg cacggttgac gacccggtgg gcaaggttga cctggctgcc
     3661 gtagtagtcc ccgtccctga acacgactgc cccgtggtgg atcccggccc ggggtttggg
     3721 ccggtctgcg aacagagcga ggaaaccgac cgcccactcg gccagtgacc cgggatcggg
     3781 cgagacgacc atgacctcgt cgccgatcgt cttgacgatc ccggcttccg gcggcagggt
     3841 cgcctcgacc gtttcggtga actgttcgac gaccgagaag gcggtctcgt cgccttcctg
     3901 ctcggtgaag cgggtgaagc cggtcaggtc gatgaagcag agtgtggtcc ggacctggtc
     3961 gaggttggtg tcgccgccga agtcggactc catgtggccg atcacgtcct gctcgaggaa
     4021 gtacctcagg tagcggtcgt gaaggtattc gaagagcggc accgcggtcg gcgaggtccg
     4081 tcgggccagg tcgccaagct cttcggccat ctcgatttcg ctgagtccgt cgcggatcat
     4141 cggctcgtgg acgaagagat ggaagagcct cacttccgca tcggcgatgc gtcggaccgc
     4201 ctgggcgtaa accctcgtga gctgcagcat cgcctcgacc gggagtccgg cttcgaccac
     4261 ctcccccatc ctccggaagg cttcgatgtc ccgggccgag agggtcatct cgcggtccgc
     4321 aggggtgccc atcacggtca tcgcccgttc gatcacctct acgctcaggc cggtctcttc
     4381 cgcagccgtc gcaaggtcga ccctttcgct cttgtcggcg aaaaggtcct ccgccatccc
     4441 gaaagagagc ttcccttcct tgccggcttc cttcaattcc tccagggaat gcccccgctc
     4501 gcgcatccgg gcgacgatgc gggcctgcgc ggcagccgca ggtgtccacc gtcccttcct
     4561 caccgggacg accttctcgt cggcccagcg ggtcagtgtg gctggcgaga ccccggcccg
     4621 gcgggccgcc tcattgaggc tgattcgatc gtcgccgggc attggcagca atcgtataaa
     4681 tcaaatcggc ttccctccga attcgttgca tacctctgcc ccgagaattt cccgaacctg
     4741 cgagtaatcc tcgctccagc ccagggccca catcagcttg accagggcca actcgggaat
     4801 gacgggcagc aggaccgcgc cggtctcctc gagcagaagc ttcgactcat aggcgttctg
     4861 aacccccaca ggctgggcgt tcatggtcag tgcgagcact atctcgtgtt cggcacaggt
     4921 ctccgcgaac cggggaacgg aagacttctc cggctcaaag gggccggtga gagccggata
     4981 gagggtcatt accgcgccgc ggtacccctc gcagatcagt gtgtcggcga gcgccttgag
     5041 attgatcccc gggtgaaggt cgatcctcat cacgcggtcg tcggcggcga tcctgaagga
     5101 gtccggtcca tcctgcgggg tcccggtcgc cgaccggttc ttcagggtcc attcgaacga
     5161 gccccagctg acttcggcca cgtggacccg gccggccgag atgaaggcgg ggcctgatcc
     5221 cctcgccttt cgcaccgaaa ccccgtcgtg gacccggctc ttcgcgttcg ggcggccggc
     5281 gaatgacagg tagacgcccg gcgagaggtg ctgcagggcg atcatcgcgt cggcgatgtt
     5341 ggtgtccgcg tcggtatccg gctcgttcgg cgggcggttc gagccagtca gcacaaccgg
     5401 caccttgata ccggccagca tgaaggagag cgcggccgac gtgaacgaca tggtgtcggt
     5461 cccgtggaga acgagtatcc cttcggggtc ctccttcgcg atgagccgct taatctcccg
     5521 gccgaccgcc acccagtggt cgggagtcat gttctccgac agcatgttca ggggctgacg
     5581 cagaatggtt tcgatgccgc ccttctccag gacggcgctc acatgggggc cggcgaggat
     5641 gtccagctgg tcaccggctt cactcagccg gaccagtttc tcgcggcccg agaggggctc
     5701 ggagcgcgag cccacggtgc ccccggtcac cagtactccg attctgtgcc ggtcggccac
     5761 gaagccagag tatctggccg gggctggtcg ggttgccgaa gtcaagggtg gccgaactca
     5821 agtccagaca acggacaagg gcggcggggc acaagtgccc cgccgcccga tccgtgtcag
     5881 cccatcgcta ctcgtctaga tggacctggg ttacttcgtc tcctcgctcg ccggtcctga
     5941 tcctgatcga ct
//
LOCUS       DAOTIP010000023         5863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_16163_length_5863_cov_3.446281, whole genome shotgun sequence.
ACCESSION   DAOTIP010000023 DAOTIP010000000
VERSION     DAOTIP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16163_length_5863_cov_3.446281"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="P5138_00875"
     CDS             complement(<1..393)
                     /locus_tag="P5138_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59161.1"
                     /translation="MTDSLDSQTPELRAINTIRTLSIDAIDKANSGHPGTAMALAPLA
                     YKIWQEYLRFDPADPHWPNRDRFVLSAGHASMLIYSVLHLAGVKKVDEDGTVSDRLSV
                     TLEDIENFRQLGSSTPGHPESHLTTGVET"
     gene            complement(472..978)
                     /locus_tag="P5138_00880"
     CDS             complement(472..978)
                     /locus_tag="P5138_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRV59162.1"
                     /translation="MSFQPSPGEQIIFQGHPSWRSILALYLKGLLIGLIIAVIAKLLG
                     EGFATVFIIVLAALGIAVLIGFIKRVATVYTITNRRLNIKRGIFARDIQETRLERVQN
                     VNYSQTVFQRLLQVGDVDFDTAATDEDNLFTFNGVADPQHVVTEVDKATSLGASGSHG
                     LGEPSSSY"
     gene            1065..3254
                     /gene="priA"
                     /locus_tag="P5138_00885"
     CDS             1065..3254
                     /gene="priA"
                     /locus_tag="P5138_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRV59163.1"
                     /translation="MSIARVEPLTTARALRGPFDYLIPDRLGDISVGTVLEVPFGRRR
                     ITGVVIEITDTTEVPPEKLVEPIRVLGTGTNKDLVGLGRWIATEYCSTPARGLGLVLP
                     PGTGTGGERTRFRVEKLNSITDAGREALEGGVRLGAKQQLALQQLLEGDLTGRQLAAR
                     AGIDSATLKRLEERELIERQQVHVRRRPEGSGVGSAAATAPELTPAQAAAVRALVSKL
                     DDPEAGEGVLLAGVTGSGKTEVYLAVVEEVLARGRTAIVLVPEIGLTPQAVGRFQARL
                     GDRVAVLHSALTAGQRFDEWQRLKAGDASVCVGPRSAVFAPLENLGLIVIDEEHDSSY
                     KQEGDPRYDARDVARRRARETGSLLVLGTATPRPESWERLERIDLPARVDEQGMPPVE
                     LVDMREADPRQGPIHRRTWEALNEVREKRQKAIVMINRRGFAPWLTCRTCGHHWGCPN
                     CDVSLIVHRESDQLICHHCNHAEPLPRRCPECGGTTLSQTGAGTQRIERLLVEELAPM
                     PVFRLDADTSAGPGGHARILSAFDQEESAILVGTQMVAKGHDFPEVTLAAILDADATL
                     RFPDFRAEERTFSLVTQLAGRSGRSRAGGRVIVQTLAPGNPSISHAATHDSTGFLAGE
                     LERRRELGYPPFSHLIRIQLAAEEEPEVERAADLVAARLDAALPAGAELLGPAPMFRA
                     RNRFRRRILVRSTTRAESIEAVHSTIEELLADKFLRNITLAIDVDPQ"
     gene            3277..3966
                     /gene="def"
                     /locus_tag="P5138_00890"
     CDS             3277..3966
                     /gene="def"
                     /locus_tag="P5138_00890"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRV59164.1"
                     /translation="MASEDETATLSEEAAEYAEGMERAEEALAEAAAEPEVEPEGGEE
                     PTGRFDEETLRRREAALAQIVQYGDPMLKSRSSEVTDFDEALALEIERMFELMKDGLG
                     VGLAAPQAGKLRRLLVFQTNADAIPQELVNPEIEWLSDETEVAAEGCLSIPRVIVDVD
                     RPLYARVRGRDRSGAEVLIEASGHEARVLQHEIDHLDGVLILDRTEKKQRKGAMKALR
                     RGESYSPHEED"
     gene            3974..4873
                     /locus_tag="P5138_00895"
     CDS             3974..4873
                     /locus_tag="P5138_00895"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRV59165.1"
                     /translation="MSGVYLGTSEFAATVLGILAGSDQGPELVVTLPDRRRGRGRREQ
                     ASPVADKAEELGIEVLKSGNVNEGDDLDRILEVGGDWASICAFGQLIKEPLLSELPML
                     NVHPSLLPRWRGAAPVERAIMAGDPKTGVCVMRLVAGLDSGPVALREETGIDPDEDFG
                     SLSARLAEIGGRLLVEALASAGRGGIDWQEQTEEGLAYAEKISAEDREINPSRSAGQI
                     HDQVRGLTPHIGAWLPLEGDQRLGIRRTTVLGAGPAAGEFGETDGWPVLGCGEGAIRL
                     DRVQPPGKKEMDGDAWLRGHGLP"
     gene            4901..>5863
                     /locus_tag="P5138_00900"
     CDS             4901..>5863
                     /locus_tag="P5138_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRV59166.1"
                     /translation="MSLVTPERQVAFEVLRRTFERDEHTEAAFREEAAARELTGRSRA
                     QAQRLSYGAVQRRGTTDWVVASFMKQKSRRPDPAVAAALRLGIFELLFSDGTPDHAAV
                     DQAVSLVRAAGADHAAGFANALLRRTLRERDSLAAKLADDSTPEKAAVAYSIPEWLAR
                     LWWDELGAERARALMDAANRPSERAVRVNTARISPEQAIERFSGPDLELNRAEGPWPM
                     APPELLIARGSLAAIEEAAEEGLVVAQSRGSAAVVEVLDPQAEERVLDLCAGPGIKTG
                     QIAARIGRMGNMVSVEPVESRAEDVAAQLERLGFHHGLVVEADAR"
BASE COUNT          994 a         1893 c         2100 g          876 t
ORIGIN      
        1 ggtctcaacc ccggtggtca ggtgcgattc cggatggccc ggggttgagg agccgagctg
       61 gcggaagttc tcgatgtcct cgagcgtgac cgagaggcgg tcggagaccg tgccgtcttc
      121 atcgaccttc ttcacccccg ccaggtgcag gaccgagtag atcagcatcg aggcgtggcc
      181 ggcggagagc acgaagcggt cacggttcgg ccaatgcgga tcggccgggt cgaagcgcag
      241 gtactcctgc cagatcttgt aggccagtgg ggcaagggcc atcgcggtcc cgggatgacc
      301 ggagttggcc ttgtcgatcg cgtcgatcga aagggtccgg atggtgttga tggcgcggag
      361 ttcgggcgtc tgggaatcga gggagtcggt cacggacgcc atcctatgaa aacggcccgc
      421 aatcagggag cgggaaaccc ggactttgcg tgattggacc ggggccgggg ctcagtagct
      481 gctgctgggc tcgccgagcc cgtgggagcc gctggcaccg agcgaggtcg ccttgtccac
      541 ctcggtcacc acatgctgag gatcggcgac cccgttgaag gtgaagaggt tgtcctcgtc
      601 ggtcgccgcg gtgtcgaaat cgacgtcgcc gacctggagc agccgctgga agacggtctg
      661 gctgtagttg acgttctgca ccctctcgag ccgggtctcc tggatgtccc gggcgaagat
      721 gccgcgcttg atgttgagcc ggcgattggt gatcgtgtag acggtcgcca cccgcttgat
      781 gaaaccgatc aagaccgcga ttcccagggc ggccaggacg atgatgaaga cggtggcgaa
      841 cccttcgccc agcagcttgg cgatcaccgc gatgatcagg ccgatcagca ggcccttcag
      901 gtaaagcgcg aggatcgagc gccaggaagg atggccctga aagatgatct gttcgccggg
      961 gcttggttgg aacgacacgg cgcgagcata ttcgaccggg ccggtttcgt gtggaggatc
     1021 gatcccggcc tccgctcgtc cgtccgcccc cgtaccctca accggtgtcc atcgccaggg
     1081 tcgagccgct gacgacggcc cgcgccctgc ggggcccgtt cgactacctg attccggacc
     1141 gcctgggcga cataagcgtg ggcacggtgc tcgaggttcc cttcggccgg aggcggatca
     1201 ccggggtggt gatcgaaata accgacacca ccgaagtgcc cccggagaag ctggttgagc
     1261 ccatccgcgt actcggcacg ggcaccaaca aggacctggt cgggctgggt cgctggatcg
     1321 cgaccgaata ttgctcgacc ccggcccggg gactcggact ggtcctgccc cccggcaccg
     1381 gcaccggtgg cgagcggacc cgcttccggg tcgagaagct gaactcgatc accgacgcgg
     1441 gtcgcgaagc actcgagggt ggagtccggc tcggggcgaa gcagcaactg gcccttcaac
     1501 agctgctcga gggcgacctc accggccgcc agcttgccgc ccgggccggc atcgactccg
     1561 ccacgctgaa acgcctggaa gagcgggaac tgatcgaaag acagcaggtt catgtgcggc
     1621 ggcgccccga aggttccggc gtcggatcgg ccgcggcaac ggcccccgaa ctgacgccgg
     1681 cccaggcggc agcggtgcgg gcgctcgttt cgaaactgga cgatcccgaa gcgggagagg
     1741 gagtgctgct ggccggggtc accggttccg gcaagaccga ggtctacctg gcggtggtcg
     1801 aggaagtcct cgcccggggc cggaccgcga tcgtgctggt cccggagatc ggcctgaccc
     1861 cgcaggcggt gggacgcttc caggcccgcc tcggtgaccg ggtggcagtt ctccactcgg
     1921 ccctgaccgc cggccagcgc ttcgacgagt ggcagcggct gaaggccggt gacgcatccg
     1981 tctgcgtcgg accgaggagc gcggtcttcg cgccgctgga gaacctgggg ctgatcgtga
     2041 tcgacgagga gcatgactcc tcctacaaac aggagggcga cccccgctac gacgcccggg
     2101 acgttgcgag gcggcgggcc agggagaccg gctcgctcct ggtcctcggt accgccacac
     2161 cgcggccgga gagctgggag aggctggagc ggatcgacct ccccgcccgg gtcgacgagc
     2221 agggaatgcc gccggtcgag ctggtcgaca tgcgcgaggc cgaccccagg cagggtccga
     2281 ttcaccgccg cacctgggag gcgctgaacg aggtgcgcga gaaacggcag aaggcgatcg
     2341 tgatgatcaa ccgccgcggc tttgcgccct ggctgacctg ccgcacctgc gggcaccact
     2401 ggggctgtcc gaattgcgat gtctcgctga tcgttcacag ggaatcggac cagctgatct
     2461 gccaccactg caaccacgct gaaccgctgc cacggcgctg cccggagtgc gggggtacca
     2521 cgctctccca gaccggagcc ggcacccagc ggatcgaaag gctgctggtc gaggaactcg
     2581 ccccgatgcc ggtcttccgg ctcgatgccg acaccagcgc cgggccggga ggtcacgccc
     2641 ggatcctttc cgccttcgac caggaggaga gcgcgatcct cgtcggcacc cagatggtcg
     2701 cgaagggaca tgacttcccc gaggtcaccc tggccgcgat cctcgacgcg gacgcgaccc
     2761 tgcgcttccc tgacttccgg gccgaggaga gaaccttctc gctggtcacc cagctggccg
     2821 gccgcagcgg ccgcagcagg gccggaggtc gtgtgatcgt ccagacactt gcccccggga
     2881 acccgagcat cagccatgct gccacccacg actcgaccgg gttcctggcc ggcgagctcg
     2941 aacggcgacg ggagctcggc tacccgccct tctcgcatct gatccgcatc cagctcgcgg
     3001 cggaagagga accggaggtg gaacgcgccg ccgacctggt cgccgcccgc ctcgatgccg
     3061 cccttcccgc cggggcggag ttgctgggcc cggcaccgat gttccgggcc cggaaccggt
     3121 tccgccggcg catcctggtc cgctcgacca cccgggcgga atcgatcgaa gccgttcatt
     3181 ccacgatcga agagttgctc gccgacaagt tcctgcgaaa catcaccctc gcaatcgacg
     3241 tcgatcccca gtagctcgtc ggtagactcg gcggcgatgg cttccgagga tgaaacagcc
     3301 accctgagcg aagaagcggc cgaatatgcc gagggcatgg aacgggccga agaggccctg
     3361 gccgaagcag ccgcggaacc cgaagtcgag cccgagggcg gcgaggagcc gaccggacga
     3421 ttcgacgagg aaacgctcag gagacgggag gcggcgctcg cccaaatcgt ccagtacggc
     3481 gatcccatgc tgaagagccg ctcctcggag gtgactgatt tcgacgaggc gctggcactc
     3541 gagatcgagc gcatgttcga actgatgaag gacggcctcg gggtcgggct ggcggccccg
     3601 caggccggca agctccggcg cctgctggtc ttccagacca acgccgacgc cattccgcag
     3661 gaactggtca acccggagat cgaatggctc tccgacgaga cggaggtcgc cgcggaaggc
     3721 tgcctcagca tcccccgggt gatcgtcgac gtcgaccgtc ccctttacgc gagagtccgc
     3781 ggccgggacc gcagcggagc cgaagtgctg atcgaggctt ccggtcacga ggcccgggtg
     3841 ctccagcacg agatcgacca tctcgacggg gtactgatac tcgaccggac cgagaagaag
     3901 cagcgcaagg gtgcgatgaa ggcgctccgt cgtggcgaga gctattcgcc gcacgaagaa
     3961 gactgaggcg gccatgtcgg gcgtctatct gggcacctcc gaattcgcgg ccaccgtgct
     4021 gggaatactt gctggctccg accagggtcc tgaactggtc gtgaccctgc cggatcgtcg
     4081 tcgtgggcgc ggccgcaggg aacaggcttc tccggtcgcc gacaaggccg aggaactggg
     4141 catcgaagta ctgaagagcg gcaacgtgaa cgagggtgat gacctggacc gcatcctcga
     4201 ggtcggtggc gattgggcct cgatctgcgc cttcggccag ttgatcaagg aaccgctgct
     4261 gagtgagctg ccgatgctga atgttcatcc ttcgctgctg ccacgctggc gcggggccgc
     4321 tccggtcgaa cgggcgatca tggccggcga cccgaagacc ggggtctgtg tcatgcggct
     4381 ggtcgccggg ctcgactcgg gtccggtagc gctcagggag gaaaccggaa tcgatccgga
     4441 tgaagacttc ggctcgctct cggcccgtct ggcggagatt ggcggccggt tgctcgtcga
     4501 ggcgctggcc tcggccgggc gcggcgggat cgactggcag gagcagaccg aggagggcct
     4561 ggcctacgcc gagaagatct cggccgaaga ccgggaaatc aatccgtcac ggtctgccgg
     4621 ccagatccac gaccaggttc gcggacttac accgcacatc ggggcctggc tgccgcttga
     4681 aggggaccag cgccttggta tccgccgaac cacggtgctc ggggccggac ccgctgccgg
     4741 cgaattcggg gagacagacg ggtggccggt tctcggctgc ggtgagggcg caatccgcct
     4801 cgaccgggtg cagccgcccg gcaagaagga gatggatggc gacgcctggc tgagaggtca
     4861 cggccttccg tgatcaggat ggttccgtga gcagggtcgc gtgagtctgg tcacgcccga
     4921 gcggcaggtc gccttcgagg tcctgcgacg caccttcgaa cgggacgagc acaccgaagc
     4981 ggcctttcgg gaggaagcgg ccgcccggga gttgaccggc cgctcgcggg cccaggcgca
     5041 gcggctctcc tacggggcag tccagcgccg cgggacgacc gactgggtcg tcgcctcttt
     5101 catgaagcag aagtcgcgcc ggcccgaccc cgcggtcgcg gcggcactcc ggctcggcat
     5161 cttcgagctg ctcttctccg acggcacccc ggatcacgcg gcggtcgatc aggcggtgag
     5221 cctggtccgg gccgcgggtg cggaccacgc ggccggcttc gccaatgcgt tgttgcgacg
     5281 gaccctgcgt gagcgtgact cactggcggc gaagttggcc gacgactcga cccccgagaa
     5341 ggcggcggtc gcgtactcga ttccggaatg gctggccagg ctctggtggg acgagctggg
     5401 cgccgaacgg gcccgggcgc tgatggacgc agcgaacaga ccgtcggaaa gggcggtgcg
     5461 cgtaaacacg gcccggatca gcccggagca ggcgatcgaa aggttctccg ggcccgacct
     5521 ggaactgaac cgggccgaag gaccctggcc gatggccccg ccggagttgc tgatcgccag
     5581 ggggagcctc gccgcgatcg aggaggcagc cgaagaaggg ctggtcgtgg cccagtcaag
     5641 gggatcggcc gccgtcgtcg aggtgctcga cccgcaggcg gaggagaggg tcctcgacct
     5701 ctgcgccggt cctggaataa agaccgggca gattgccgcc aggatcgggc ggatgggcaa
     5761 catggtctcg gtcgagccgg tggagtcccg ggccgaggat gtcgccgcgc agcttgagag
     5821 gctcggtttc catcacgggc tggtcgtcga ggctgacgcg cgg
//
LOCUS       DAOTIP010000024         5833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_16252_length_5833_cov_3.768951, whole genome shotgun sequence.
ACCESSION   DAOTIP010000024 DAOTIP010000000
VERSION     DAOTIP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16252_length_5833_cov_3.768951"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="P5138_00905"
     CDS             <1..986
                     /locus_tag="P5138_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59167.1"
                     /translation="LGFLSDRTVLPADSQFPSPDLPTPQRTDIQLTGNLTGDQITIPA
                     TTNTGLQFPYMHMMHPLEPDLKIPMTFRLNDPGLTGTWDEATGAMTLSGNLDIIVVTG
                     TGTGFPVPDSLDDAGVPPLGLFARCRFDDVPVSFSTATTSPFTGQPFANGFGKDGAMT
                     TSWDSLAPSVSENGGDCGDLNQITTSSGGIWLSNGVVEPIAQPGPPDPTCETDLSLCP
                     PPKYIEIDAVRLRPGHRTVKRGRKVMLTVRVHNSGNKAARKLKVKIKSSSKAFKVPKF
                     VRLTVPAGKSARKKFAVKVKRNAKRTRITAVNNGWAGRSYLKVKPKRKHRR"
     gene            1016..2266
                     /locus_tag="P5138_00910"
     CDS             1016..2266
                     /locus_tag="P5138_00910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRV59168.1"
                     /translation="MRESETRPEQHLSDPGKEARRKAGIPVVPAFDGYRALAILAVVA
                     LHLTQISGLAGDGGTLGSRVIWGTLGRAVEILFIVSGFVVFLPTVARGGSFGSVRAYA
                     IRRAARLLPAYWLTLLICLLLIGLFNLENPLIPGEPITFPGADDLLFNFSATVVPAKM
                     FIDVLPVGFGLDPPMWTLSSELIFYIVLPLIALAFFRFPKTGLLISALITLGWALAFD
                     NIATIANDLGAGIGLGESARLKLASYQQFPAWAFSFGLGMAGAVAWVRLSGRPRPGLN
                     RRAGIVALVSLAAVAICALRLGADGAWIHTSLLWSTAFSASIAMLMLSLAMTRDTWQK
                     PFTAEPVRRLGDISYGIYLVHYPLIVLVLSAIDLSGNSSLTRFLVLAAIVLPGAVLYG
                     YLSARFLEQPIRRWARRYGRRDEG"
     gene            2292..3860
                     /locus_tag="P5138_00915"
     CDS             2292..3860
                     /locus_tag="P5138_00915"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRV59169.1"
                     /translation="MRKKNPETHDEKLDYLRELRDAAINSASEEAVEKHHAKGKLTAR
                     ERIGKLLDPGSFEELDTFVRHRTYDFEMNKRRPWGDAVVTGHGTIDGRKVFVFSQDFT
                     VFGGSLGEVMAEKMCKVMDLAAKVGAPVIGLNDSGGARIQEGVVSLGAYGDVFARNVS
                     SSGVIPQISVIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPEVIKTVTGEEVGFEE
                     LGGAMSHNSKSGVAHFAAEDEESCLEDVRYLMSFLPLNNMEQPPHSIPADDPDREDPE
                     LDTFVPDDPNKPYDMRKVVEKIVDDEEFFEVHEHFAKNIICGFSRLDGHPVGIVGNQP
                     AFKAGVLDIESSEKAARFVRTCDAFNVPIVTFTDVPGFMPGTDQEWNGIIRHGAKLLY
                     AYTEATVPKLTVVTRKAYGGAYDVMASKHMLADFNFAWPTAEIAVMGAEGAVNIIYRH
                     DIKNSPTPEERRETLIADYKAHFANPYAAAERGYIDDVIEPSQTRPKLIKALRTLQTK
                     RVAQPRRKHGNIPL"
     gene            4934..5419
                     /locus_tag="P5138_00920"
     CDS             4934..5419
                     /locus_tag="P5138_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HRV59170.1"
                     /translation="MVWKLGGTNTPKNLKVLGDPLRTYPFGGQHDARLDNRGYLSVFD
                     NGTNLNRPPRVVRYRIDQKTGTATLVSSFTDPRVQLSACCGSARRLPGGGWLVNWGGI
                     GPTSDYGHYGLIGAYTGKGKPIFRLRVKGTHPYRVQPITGRYPQLGRLRNGMDTIDRN
                     S"
     gene            5481..>5833
                     /locus_tag="P5138_00925"
     CDS             5481..>5833
                     /locus_tag="P5138_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59171.1"
                     /translation="MALLLGACGGEDGSARVKEPVVSVSPSLKPAFRWNQSDYAVRCK
                     SGPVEVTVDAPSGWRVAVGGRKPRTGSFDLKQPLRADRSFEFKVTNSAGTTKKFHVRC
                     LPDDFPAYRIERYRPG"
BASE COUNT         1064 a         2011 c         1745 g         1013 t
ORIGIN      
        1 gcctcggctt tctttccgac cggaccgtgc tcccggccga cagccagttc ccctcgcctg
       61 acctgcccac gccccagcgg accgacatcc agctgaccgg caacctgacc ggcgaccaga
      121 tcacgattcc cgccaccacg aacaccggtc tccagttccc ctacatgcac atgatgcatc
      181 cgctcgagcc ggacctgaag atcccgatga ccttccgcct caacgatccc ggcctgaccg
      241 gcacctggga cgaagcgacc ggggcgatga cgctgagcgg caacctcgac atcatcgtcg
      301 tgaccggtac cggcaccggc ttcccggttc cggattcgct cgacgacgcc ggcgttccgc
      361 cgctcggact ctttgcccgc tgccggttcg atgatgtccc ggtctcgttc tcgactgcga
      421 cgacgagtcc cttcaccggc cagcctttcg ccaacggctt cggcaaggac ggcgccatga
      481 ccacgagctg ggactccctt gctccctcgg tctccgagaa cggcggcgac tgcggcgacc
      541 tgaaccagat cacaacctcg agtggcggca tctggctctc caacggtgtg gtcgagccga
      601 tcgcccagcc cggcccgccg gatccgacct gcgaaaccga cctcagcctc tgcccgcccc
      661 cgaagtacat cgagatcgac gcggtcaggc tgcgacccgg acaccggacc gtgaagcggg
      721 gcaggaaagt gatgctgacc gtccgggtcc acaattccgg caacaaggcc gccaggaagc
      781 tgaaggtgaa gatcaagtcc agcagcaagg cgttcaaggt gccgaagttc gtgaggctga
      841 cggtacccgc cggcaaatcg gcccggaaga agttcgcggt gaaggtgaaa cggaacgcga
      901 agcggacccg gatcactgcg gtcaacaacg gctgggccgg ccgctcctac ctgaaggtca
      961 aaccgaagcg gaagcaccgg cgctgagctg agccctggtt ctagactccc tccgtgtgag
     1021 ggagtcagag accaggccgg agcaacatct ctccgacccg ggcaaggaag cccgacggaa
     1081 agcgggaatc cccgttgtac ccgcgttcga cggctaccgg gcgctggcca tccttgccgt
     1141 agtcgccctt cacctgaccc agatctccgg tctggcgggc gacgggggaa cattggggtc
     1201 ccgtgttatc tggggcaccc tcggtcgggc ggtcgagatc ctcttcatcg tcagtggctt
     1261 cgtggtcttc cttccgaccg tcgccagggg cggcagcttc ggaagcgtca gggcttacgc
     1321 gatccgtcgg gcggcgcgtc tgttgcccgc ctactggctg acgctcctga tctgcctgct
     1381 gctgatcgga ctcttcaacc tggaaaaccc gctgatcccg ggagagccga tcaccttccc
     1441 cggggccgat gacctgctct tcaacttctc cgcgacggta gtaccggcca agatgttcat
     1501 cgacgtactg ccggtgggct tcgggctgga cccaccgatg tggacgctgt cctcggagtt
     1561 gatcttctac atcgtcctgc ccctgatcgc gctggccttt ttccgtttcc cgaagaccgg
     1621 cctgctgatc tccgccctga tcaccctggg ctgggcactc gctttcgaca acatcgccac
     1681 aatcgccaat gacctggggg caggcatcgg tctgggcgaa agcgcgcgtc tgaagctcgc
     1741 ctcctaccag cagttcccgg cctgggcctt ctctttcggc ctcgggatgg ccggtgcggt
     1801 cgcctgggtc cgcctgtccg ggagaccgag acccggcctg aatcgccggg cgggcatcgt
     1861 cgcgcttgtc tccctggcgg ccgtggctat ctgcgccctg cggcttgggg ccgacggggc
     1921 ctggatccac acttcactcc tctggtcaac ggccttctcc gcttcgatcg ccatgctgat
     1981 gctttccctg gccatgaccc gggacacctg gcagaagccc ttcactgccg aaccggtgcg
     2041 tcgcctgggc gacatcagtt acggcatcta cctggtccac tacccgctga tcgtcctcgt
     2101 tctctctgcg atcgacctct cggggaactc ttcactgacc aggttccttg tgcttgcggc
     2161 aatcgtcctg ccgggcgcgg tcctttacgg gtacctctct gccaggttcc ttgaacagcc
     2221 gatccggcgc tgggcgcgca ggtacggtcg cagggacgaa ggctgagtga acaaaataga
     2281 ctgcttcctc catgcgtaag aagaatccag agacccacga cgagaagctc gattacctgc
     2341 gcgagctgcg tgacgccgcg atcaactccg cctccgaaga ggccgtcgag aagcaccatg
     2401 ccaagggcaa gctgaccgcc cgcgagcgga tcgggaagct gctcgacccg ggctccttcg
     2461 aggaactgga caccttcgta cggcaccgga cctacgactt cgagatgaac aagcgccgtc
     2521 cctggggcga tgctgtggtc accggccacg gcacgatcga cggccgcaag gtcttcgtct
     2581 tctcccagga cttcaccgtc ttcggcggct cgctcggcga ggtgatggcc gagaagatgt
     2641 gcaaggtgat ggacctcgcc gccaaggtcg gagccccggt aatcggtctc aacgactccg
     2701 gcggcgcccg catccaggaa ggcgtcgtgt ccctcggcgc ctacggtgac gtcttcgccc
     2761 gcaacgtctc gagctccggg gtgattcccc agatcagcgt gatcatgggg ccctgcgccg
     2821 gcggcgccgt ctactccccg gccatgaccg acttcatctt catggtcaag gagacctcgc
     2881 acatgttcat cacgggtccc gaggtgatca agaccgtgac cggcgaggaa gtcggcttcg
     2941 aggaactcgg cggcgcgatg tcccacaact ccaagtccgg cgtcgcccac ttcgccgccg
     3001 aggacgagga gagctgcctc gaggacgtgc gttacctgat gagcttcctg ccgctcaaca
     3061 acatggagca gcctccccat tcgattccgg ccgacgatcc tgaccgggag gatccggaac
     3121 ttgacacttt cgttcccgac gacccgaaca agccctacga catgcgcaag gtcgtggaga
     3181 agatcgtcga cgacgaagag ttcttcgagg tccacgagca cttcgccaag aacatcatct
     3241 gcggtttctc ccgcctcgat ggccatcctg tcggaatcgt cggtaaccag ccggctttca
     3301 aggccggggt gctggacatc gagtcctccg agaaggcggc ccgcttcgtc cggacctgtg
     3361 atgctttcaa cgtcccgatc gtcaccttca ccgacgttcc cggattcatg ccgggcaccg
     3421 accaggaatg gaacgggatc atccgccacg gggccaagct gctctacgcc tacaccgagg
     3481 cgaccgtgcc gaagctgacc gtggtcaccc gcaaggccta cggcggcgcc tacgacgtca
     3541 tggcctccaa gcacatgctg gccgacttca acttcgcctg gccgaccgcg gaaatcgccg
     3601 tgatgggcgc cgagggcgcg gtcaacatca tctatcgcca cgacatcaag aactcgccga
     3661 ccccggaaga gcgtcgcgag accctgatcg ccgactacaa ggcgcatttc gccaatcctt
     3721 atgcggcagc ggaacgcggc tacatcgacg acgtgatcga gccgagccag acccggccga
     3781 agctgatcaa ggccctgcgg accctgcaga ccaagcgggt cgcgcagccc cgccgcaagc
     3841 acggcaacat ccctctctag acaccgaatc cggagcgatt ccggagcaac ccgggttttg
     3901 ccgggttatc ttgggcggac gccactgatg ccagggagtt tctccaagag cgcgctgcga
     3961 gggctgatca cggccgcgat tctggctgcc gttcttgcgg cagggccttc cggcgccgat
     4021 gcgaaacccg ctcaggcggc gaagccgcga ctcacaacca gtccgactgt cttcccggcc
     4081 ttttcctgga agaagcggga ctacgtggtc cgatgcgcaa ggtcgaagaa tccgcgggcc
     4141 cggatcgtcg tccccagggg atggcaggcc agaatcgccg gatccacctt ccacgcccgc
     4201 aatttcagcc acaagctgaa ttcgggcgaa agcgcgggaa cctggttctc ggtgcgacgc
     4261 aagggcagcc gcaaatcgcg gagcttccac atccgctgcc tgccagccga cttcccggcc
     4321 ttcaggttca gccggtaccg ctccggtggc ccgagactga tgttcgcgca gatcgagaac
     4381 gagtacgcag tcttcttcga ccgtcacggg gttcccgtct ggtggatgaa gaacgtggtc
     4441 ccgccgctga atccccaggt cctccccgac gggacgatct cctatctctc cctggacacg
     4501 accggcccga gaaatctcgt ctcctacgtg atcaggaacc tgaacggaag gctgatccgc
     4561 acgatcctcc ccgccggggg actgctcacc gacgcccacg aactgaggct gcttccgaac
     4621 ggcaactacc tgatcggagc ctttcggcag cagaacgcgc tcgatgcctc cgccttcggc
     4681 ggctcatccc acgcagtcgc cctgagcctc cagctacagc agctcaagcc gaacgggaag
     4741 ctcgtctgga aatggaactc gaccggccac atcaagctgg gcgagagcgg tcgctggtgg
     4801 aacaacttca ccaccaacgg ggagccgtat gacctggtcc atttcaactc ggccgacctc
     4861 gacggcaact ccctggtcat gtccttccgg cacctcgacg ccgtgatcaa ggtcagcaag
     4921 aaaaaccggc aagatcgtct ggaagctggg cggaaccaat accccgaaga acctgaaggt
     4981 gcttggcgat ccgctgcgca cctatccatt cggcggccag cacgacgcga ggctggacaa
     5041 ccgcggctat ctctcggtct tcgacaacgg gaccaacctg aaccggccgc ccagagtggt
     5101 ccgctaccgg atcgaccaga agaccgggac cgcaaccctg gtttcctcat tcaccgaccc
     5161 gagggtgcaa ctctcggcct gctgtggttc ggccagaagg ctgcccggag gtgggtggct
     5221 ggtcaactgg ggcggcatcg gcccgaccag cgactacggc cattacggcc tgatcggtgc
     5281 ctacaccggc aagggcaaac cgatcttcag actgcgcgtg aagggcacgc acccttaccg
     5341 tgtccaaccg ataaccggcc gatacccgca gctgggccgg cttcgcaacg gaatggacac
     5401 gatcgatcga aactcctgac ttcatccgaa ggggccggac atccattcgt cccgccctcg
     5461 ccgtcctggc ctgctcggga atggcgctgc tgctgggtgc ctgcggcggg gaagacggct
     5521 cggcccgggt caaggaaccg gtcgtttcgg tctcgccgtc cctgaagccc gccttccgct
     5581 ggaaccagag cgactatgcg gtgcgatgca agagcgggcc agtggaagtc acggtggatg
     5641 ctccctccgg gtggagggtt gctgtcggcg gccggaaacc ccgaacggga tccttcgatt
     5701 tgaagcagcc cctgcgcgcc gaccgttcct tcgaattcaa ggtcaccaat tcggctggca
     5761 ccacgaagaa gttccacgtc cgctgcctgc ccgatgactt ccccgcctac cggatcgagc
     5821 ggtaccgccc cgg
//
LOCUS       DAOTIP010000025         5708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_16744_length_5708_cov_2.960729, whole genome shotgun sequence.
ACCESSION   DAOTIP010000025 DAOTIP010000000
VERSION     DAOTIP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16744_length_5708_cov_2.960729"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="P5138_00930"
     CDS             complement(<1..937)
                     /locus_tag="P5138_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV59172.1"
                     /translation="MRRLKHWGWGYEDQQQPHEEVVAASEGIIPMLGFGEAPERPVGL
                     DRVELRKPRLDSPGGMFTDDRHQRTVHSLGKAYRDIVRGFYGEFDSPPDLVAFPEEEA
                     GVEEVLSRAADWGAAVIPFGGGTSVCGGVEPRLADPERPVVSLDMTRMNRLIEVDEFS
                     LAARIQAGARGPELEDQLKAHGLTLRHFPQSFEYATLGGWIVTRAGGHFATGETHVDD
                     LTESVRAITPAGEWESRRLPGSGAGPSPDRMLLGSEGILGVVTEAWMKVRPRPDYKAT
                     ATVEFPPGDEAFSAAARGAVRLIAQSRLMPSNLRLI"
     gene            complement(941..2932)
                     /locus_tag="P5138_00935"
     CDS             complement(941..2932)
                     /locus_tag="P5138_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV59173.1"
                     /translation="MDYFITGGTGFIGRYLIERLLKREGTVHVLVREGSRGRLEEMRS
                     GWGEDGERIVPVIGDLSQPNLGLDAGQVAELKGKIDAFYHLAAIYDMEADEEAIRIGN
                     IEGTINAVDLANELEVGCFNHASSIAAAGLFKGTWREDFFEEAENLDKHAYFRTKHES
                     ERIVRERLTVPWRVYRPGIVVGDSETGQMDKIDGPYYFFKLIQKLRDVLPPWAPTVGI
                     EGKQINVVPVDFVADAIDHISHEEDKDGMVFHLTDPNPLRAGQIINLFARSAHAPQMS
                     MRLDPQMLEIVPPAVRGGLMMLPPVKRITDSVLDDLGIPREVLVYINYPTKFDCTNTL
                     EALEGTDIAVPPLASYADRLWDYWERNLDPDLFKDRSLGGAIGGKVVMITGASSGIGE
                     AAAIKAAGAGAKVLLVARSRDKLEEIQTLIAEEGGEAYIHTADLSDTDDIERMADEVL
                     AEHGHVDVLVNNAGRSIRRSVALSYDRFHDYERTMQLNYFGSLKLILKLLPRMRERKS
                     GHIINVSSIGVQTNAPRFSAYVASKAALDAFSRCIGSEIIDDKCHITTIHMPLVRTPM
                     IAPTKMYDAFPAISPDEAAEMITDAMIRKPKKVATRLGNFGELMYSLSPKASDAILNT
                     GYKLFPDSKAAKGDKDGESEKAPSTEAVAFAHLLRGVHW"
     gene            3088..>5708
                     /locus_tag="P5138_00940"
     CDS             3088..>5708
                     /locus_tag="P5138_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRV59174.1"
                     /translation="MPEATAVEVTMPEMGESVTEGTVLEWHVAVGDTIEEGDTLVEVS
                     TDKVDAEVPAPVTGTVTELRAEVDEEIAVGAVLAVIDPDASGSGPSAATAVGEGDADL
                     GDSSTPEASSAPNGSAATSDAVQAEPIEVAMPEMGESVTEGTVLEWHVAVGDSVEEGQ
                     TLVEVSTDKVDAEVPAPTAGEITELLVEPDQEIPIGAVLCKMIPGAGSGGGNGAAPAA
                     TESAAPASAEPADVGDGRATPIAKRVAAAKGLKLDQVTGTGPGGKVTKSDVLAAGEGN
                     GAAPANREEKALRGPAGMLAKAMDESRTIPTATSFRTVAVDTLDAKRKGLNGALSQRG
                     MKVSFTHLVAWAIVQAARKWPVMARHFEEKDGKLFSVSDGQINLGIAVDVEKKDGSRS
                     LMVPAIKGADQLDFPAFHAYYEDLITKTRENRLTPDDFAGTNITLTNPGGIGTVASVP
                     RLMNGQGTIVAAGSIAYPPEWSHADQEKIDQLGVSKVMTLTSTYDHRIIQGAESGNFL
                     KTLDGLLAGGSEFYESVADDLGVDRTVLTAAQPKAGLAPPLSAAAAPSASAIAEAPTP
                     ELLQAVQAATSLLKAYRTHGHLAAHLDPLGAEPKGDPALDPETVHLTPELMAQVPASI
                     LRIGVPGETLLEALPRMKAAYTGSIGYQFEHISSHNQRVWLREMVETGAHRKAFDKDE
                     QKRLLGRLIDVFEFERFLEKAYLGQKMFSIEGLDSVVTMLDELTTIASRGGTKEVVLG
                     MAHRGRLSVLAHTVRRPAEAIFAEFEGGKRIEDVKAVAAIPHGGTGDVKYHYGHEGVA
                     EHHDGTSIKVHLYPNPSHLEFVNPVVTGATRYSQGDIEENRLSLDPAKAVPVVLHGDA
                     AFPGQGVVAETFNLQSL"
BASE COUNT         1013 a         1923 c         1831 g          941 t
ORIGIN      
        1 cgatcagcct caggttgctc ggcatcagcc gcgactgggc gatcagtcgg accgctccgc
       61 gtgccgccgc cgagaaggcc tcgtctcccg gaggaaactc gaccgtcgcg gtcgccttgt
      121 agtcgggacg gggccggacc ttcatccagg cctcggtgac cacccccagt attccctcgg
      181 agccaagcaa catgcggtcg ggcgatggac cggccccgga tccgggcagg cggcgcgact
      241 cccactcgcc ggccggagtg atcgcccgaa ccgactcggt caggtcatcc acatgggtct
      301 cgccggtcgc gaaatggcca ccggcccggg tcacgatcca tccgcccagc gtggcgtact
      361 cgaaggactg cgggaagtga cgcagggtga ggccgtgggc cttgagctgg tcttccagct
      421 ccgggccgcg ggctccggcc tggatccggg ccgcaagaga gaactcgtcc acctcgatca
      481 aacgattcat gcgggtcatg tcgagcgaaa ccacgggacg ttccggatcg gcaagccggg
      541 gctcgacccc gccgcagacg gaagtgccac cgccgaacgg gatcaccgcc gcaccccagt
      601 ccgcagcccg cgagagcact tcctcgactc ccgcctcttc ctccgggaag gcgaccaggt
      661 ccgggggact gtcgaactcg ccgtagaacc cccggactat gtcccgatag gccttgccca
      721 gcgagtgaac ggtccgctgg tgccggtcat cggtgaacat tccgcccggg ctgtccagcc
      781 ggggctttct cagctcgacc cgatcgagtc cgaccggacg ctccggggcc tcgccgaagc
      841 cgagcatcgg gattattccc tccgatgcgg cgacgacctc ctcatgcggc tgctgctggt
      901 cttcgtaacc ccagccccag tgcttgagcc ggcgcatcgc ctaccagtgg acgccgcgca
      961 gcagatgtgc gaaggcgacc gcctcggtcg agggtgcctt ttcggattcg ccgtccttgt
     1021 cgcccttggc cgccttcgag tccgggaaca gcttgtaacc ggtattgaga atcgcgtccg
     1081 aagccttcgg cgagaggctg tacatcagct cgccgaagtt gccgagccgg gtagcgacct
     1141 tcttcggctt gcggatcatc gcgtcggtga tcatctccgc cgcctcgtcc gggctgatcg
     1201 ccgggaaggc gtcgtacatc ttggtcgggg cgatcatcgg ggtccgcacc aacggcatgt
     1261 ggatcgtcgt gatgtggcac ttgtcgtcga tgatttcgga gccgatgcag cggctgaaag
     1321 cgtcgagcgc ggccttcgag gccacatagg cggagaaccg cggggcattg gtctggaccc
     1381 cgatcgagct cacgttgatg atgtggccgg acttccgctc tcgcatccgc ggcagcagct
     1441 tcaggatcag cttcagggaa ccgaagtagt tgagctgcat ggtccgctcg tagtcatgga
     1501 agcggtcata tgagagcgcg accgatcggc ggatcgatcg gccggcattg ttgaccagca
     1561 catccacgtg gccgtgctcg gccaggacct cgtcggccat tcgttcgatg tcatcggtgt
     1621 cggagagatc ggcggtgtgg atgtacgcct cgccgccctc ttccgcgatc agggtctgga
     1681 tctcttccag cttgtccctg ctgcgggcga ccaggaggac cttggctccg gcgcccgcgg
     1741 ccttgatcgc ggcggcctcg ccgatacccg aggaagcgcc ggtgatcatc actaccttgc
     1801 cgccgatcgc gccgccgagt gagcggtcct tgaacaggtc cgggtcgaga ttccgttccc
     1861 agtagtccca gagcctgtcg gcgtacgaag cgaggggcgg gaccgcgatg tcggtgccct
     1921 cgagggcctc gagcgtgttg gtgcagtcga acttggtcgg atagttgatg tagaccagga
     1981 cttcacgcgg gatacccagg tcatcgagca ccgagtcggt gatccgcttg accggaggca
     2041 gcatcatcag gccgccacgg accgccggag gcacgatctc gagcatctgg gggtcgaggc
     2101 gcatcgacat ctgcggcgca tgagcagagc gggcgaagag attgatgatc tggccggccc
     2161 gaagcggatt cgggtcggtc aggtggaaca ccatcccgtc cttgtcctct tcatggctga
     2221 tgtggtcgat cgcgtcggcg acgaagtcga ccggcaccac gttgatctgt ttgccctcga
     2281 tgccgacggt cggggcccag ggcggcagca cgtcgcgcaa cttctggatc agcttgaaga
     2341 agtaatacgg gccgtcgatc ttgtccatct ggccggtctc ggaatcaccg accacgatgc
     2401 ccgggcgata cacccgccag ggaacggtga gccgttcccg gacgatccgc tccgactcgt
     2461 gcttggtccg gaagtacgcg tgcttgtcga ggttctcggc ttcctcgaag aaatcctcgc
     2521 gccaggtgcc cttgaacagg cccgcggcag cgatcgagga tgcgtggttg aagcagccga
     2581 cctcgagttc gttggcgaga tcgaccgcgt tgatggtccc ctcgatgttg ccgatccgga
     2641 tcgcttcctc gtcggcctcc atgtcgtaga tcgcggccag gtggtagaag gcgtcgatct
     2701 tgcctttgag ttcggcgacc tgcccggcat ccaggcccag attaggctgg ctgaggtcac
     2761 cgatcaccgg cacgatccgt tcgccatcct cgccccagcc ggaccgcatt tcctcgaggc
     2821 gcccgcgtga tccctcacgc accaggacat gcaccgttcc ctcccgcttc agcagtctct
     2881 cgatcaggta ccgaccgatg aaaccggtgc ctccggtgat gaagtaatcc acagctcctc
     2941 ctccgagcaa tcgacgcgat tagcggacta gcggggacac tacaaccacc gtcaaaacga
     3001 ggccaagcgg aagcggcatg gatttccagg cccaacctgt ccggatgtac agaacccggc
     3061 ttgtgcaaat acatacactt gggacaaatg ccagaagcaa ccgctgttga agtgacgatg
     3121 cccgagatgg gcgagtcggt gaccgaaggg accgtgctcg agtggcacgt ggcggtcggc
     3181 gacaccatcg aggagggcga caccctcgtt gaggtttcga ccgacaaggt cgacgccgag
     3241 gtgcccgcgc cggtcaccgg caccgtcacc gaactccgtg cagaggtcga tgaggagatt
     3301 gcagtcggcg ccgttctggc agtgatcgac cccgatgcga gcggatccgg cccctcggcc
     3361 gcgaccgcgg tcggagaggg cgacgccgat ctcggcgaca gctccacccc cgaagcttcc
     3421 agcgccccga acggcagcgc cgccacatcc gatgccgtcc aggccgagcc gatcgaggtg
     3481 gccatgcccg agatgggcga atcggtgacc gaaggaaccg tgctcgaatg gcatgtcgcg
     3541 gttggcgatt ctgtcgaaga gggccagacc ctggtcgagg tctcgaccga caaggtggac
     3601 gccgaggttc ccgcgccgac cgccggcgaa atcaccgaac ttctcgtcga accggatcag
     3661 gagatcccga tcggtgccgt cctctgcaag atgattccgg gagcggggtc cggcggcggc
     3721 aacggtgccg caccggcggc aaccgagagt gcggctccgg cttccgccga gccggccgac
     3781 gtcggcgacg gccgtgccac acctatcgcg aagcgtgtag ccgccgctaa gggcctcaag
     3841 ctcgatcagg tcaccggcac cgggccgggt ggaaaggtca ccaagtccga tgtcctcgcc
     3901 gctggtgaag gcaacggcgc tgctccggcc aaccgggagg agaaggcgct tcgcggaccg
     3961 gccggaatgc tggccaaggc gatggacgag agccgcacga tcccgaccgc cacctctttc
     4021 cgcaccgtcg ccgtggatac cctcgacgcc aagcgcaagg ggctcaacgg cgcgctttcc
     4081 cagcggggca tgaaggtttc tttcacccat ctcgtcgcct gggcgatcgt ccaggccgcc
     4141 aggaagtggc cggtgatggc tcgtcacttc gaagagaagg acggcaagct cttctcggtt
     4201 tccgacggcc agatcaacct cgggatcgcc gttgacgtcg agaagaagga cggctcccgg
     4261 agcctcatgg tcccggcgat caagggtgcc gaccagctcg acttccccgc cttccacgcc
     4321 tattacgaag acctgatcac caagacccga gagaaccggc tcacccccga cgatttcgcc
     4381 ggtaccaaca tcaccctcac caacccgggc ggcatcggga cggtcgcctc ggtcccgagg
     4441 ctgatgaacg gccagggcac gatcgtggcc gccggatcga tcgcctaccc gcccgagtgg
     4501 tctcatgccg atcaggagaa gatcgatcag ctcggcgtct cgaaggtgat gaccctgacc
     4561 tcgacctacg accaccggat catccagggt gccgagtcgg gcaacttcct caagaccctc
     4621 gacggcctgc tggccggcgg aagcgagttc tacgaatcgg tcgccgacga cctcggcgtg
     4681 gatcgcaccg tcctcaccgc cgcccagccc aaggcaggcc tggcaccgcc gctttcggcg
     4741 gccgcggccc cctcggccag cgcgattgcc gaggcgccca ccccggagct tctgcaggcg
     4801 gtccaggcgg cgacttcgct gctcaaggcc tatcgcaccc acggtcacct cgccgcccat
     4861 ctcgacccgc tcggtgccga gcccaagggc gacccggcgc tggatccaga gaccgtccac
     4921 ctgaccccgg agctgatggc ccaggtgccg gcctcgatcc tccgcatcgg cgtgcccggc
     4981 gagaccctgc tcgaagcgct gcccagaatg aaggcggcct acaccggttc gatcggatac
     5041 cagttcgaac acatctcatc ccacaaccag cgggtctggc tgcgggagat ggtcgagacc
     5101 ggagcccatc gcaaggcatt tgacaaggac gagcagaagc gcctgctcgg acgcctgatc
     5161 gacgtcttcg aattcgaacg cttcctcgag aaggcctacc tgggccagaa gatgttctcg
     5221 atcgagggcc tcgactcggt cgtgacgatg cttgacgaac tgaccacgat cgcaagccgc
     5281 ggcggcacaa aggaagtggt gctcgggatg gcccatcgcg gccgactctc ggtgctggcc
     5341 cacaccgtcc gccggcctgc cgaagcgatc ttcgcggaat tcgagggcgg caagcggatc
     5401 gaggacgtca aggcggtcgc ggctatcccc cacggcggaa ccggcgacgt caagtaccac
     5461 tacgggcacg agggggtggc cgaacaccac gacggcacca gcatcaaggt ccacctctat
     5521 cccaacccga gtcacctcga gttcgtcaat ccggtggtca ccggcgcgac ccgttactcg
     5581 cagggcgaca tcgaggagaa ccgcctcagc ctcgacccgg ccaaggcggt accggtcgtg
     5641 ctgcatggcg acgccgcctt ccccggccag ggcgtggtcg ccgagacctt caacctccag
     5701 tcactgga
//
LOCUS       DAOTIP010000026         5696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_16789_length_5696_cov_3.663358, whole genome shotgun sequence.
ACCESSION   DAOTIP010000026 DAOTIP010000000
VERSION     DAOTIP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16789_length_5696_cov_3.663358"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..834)
                     /locus_tag="P5138_00945"
     CDS             complement(160..834)
                     /locus_tag="P5138_00945"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRV59175.1"
                     /translation="MAELNLILLGPPGSGKGTQGERLQEDLEYPYYATGDILRAAVKE
                     GTEVGKAAKEYMDRGDLVPDEVIIGIIAERLESGEADHGFILDGFPRTVVQAEALDAE
                     LGQLGRQLTAVILIDVDDEEIVNRLSGRRVCQEKGHVYHVDHNPPQQEGICDVDGSEL
                     YIRDDDKPEVVRHRLEQYQEKTAPLVDYYKKQGLLNKVEGADDPDRVSEKIRTLVSTL
                     SLEEGS"
     gene            complement(845..2116)
                     /gene="secY"
                     /locus_tag="P5138_00950"
     CDS             complement(845..2116)
                     /gene="secY"
                     /locus_tag="P5138_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRV59176.1"
                     /translation="MIATIAKAFSVPEIRRKLLFAAAMLALYRLGAYIPAPGIDVDAV
                     KELESQLTGSNILGFLNLFSGGSLSRLSLFALGIMPYITASIILQLLTVVVPSLERLQ
                     KEGEVGQQKITQYTRYLTVGLAAMQSFAYVFLFNSLSAGGNSVVSNTNPANVLLIVIC
                     LTAGTTLLMWIGELITQRGIGNGISLLIFASIVSGVLPGIQTWWNNPDQVFVVMLPFI
                     VLGVIVAIVFVQEGQRRIPVQYAKRVVGRKMTSGGSTYLPLRVNMAGVIPVIFAASIM
                     ALPPTIGQLINTPAALDFAAFLSPSSWAYVVGEVFFIIVFTYFYTAVTFNPVDQADNL
                     KKYGGFIPGVRPGRPTAEYLDRVLSRLTFPGALYLGAVAAFPTIFFNNVGGGGLFFGG
                     TSILIVVGVALDTVKQLEAQLMMRNYEGFLR"
     gene            complement(2116..2583)
                     /gene="rplO"
                     /locus_tag="P5138_00955"
     CDS             complement(2116..2583)
                     /gene="rplO"
                     /locus_tag="P5138_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRV59177.1"
                     /translation="MGLHNLSPKPGSRRPKKRIGRGPGSGTGKTSGRGHGGQGQRSGN
                     KRKVNLEGGQTPIHMRMRKLRGPHMKKSMPFENFRTSTQPVNLGDLEDRFDSGAEVTP
                     ESLKAAGLATRNEPVKILARGEISKSLTVKAHGFSAAAKEKIEAAGGSCELID"
     gene            complement(2616..2795)
                     /gene="rpmD"
                     /locus_tag="P5138_00960"
     CDS             complement(2616..2795)
                     /gene="rpmD"
                     /locus_tag="P5138_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRV59178.1"
                     /translation="MAKLSITQIRSEINTNPNQRGTLAALGLGKRGRSVERDDSPQLQ
                     GQLKVVSHLVKVEEA"
     gene            complement(3266..3751)
                     /gene="rpsE"
                     /locus_tag="P5138_00965"
                     /pseudo
     CDS             complement(3266..3751)
                     /gene="rpsE"
                     /locus_tag="P5138_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002063426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(3806..4159)
                     /gene="rplR"
                     /locus_tag="P5138_00970"
     CDS             complement(3806..4159)
                     /gene="rplR"
                     /locus_tag="P5138_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRV59179.1"
                     /translation="MTVITKRQRRQRRRYRVRAKVSGTAQRPRLSVYRSNRGVFAQLI
                     DDVDGKTLAAANWTEPGLKELRGTDQAKKAGELLAERAKKAGVETCVFDRSGYRYHGR
                     VKALAEGAREGGLEF"
     gene            complement(4156..4689)
                     /gene="rplF"
                     /locus_tag="P5138_00975"
     CDS             complement(4156..4689)
                     /gene="rplF"
                     /locus_tag="P5138_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRV59180.1"
                     /translation="MSRIGRKPISLPDGVEIDIKPGLITVKGPKGELTQEISTDMTVK
                     TEDGTITIERPTDRPSHRALHGLTRSLIANMIVGVTDGYQKVLEIQGVGYRAAAKGKN
                     LELALGYSHPVSIEAPEGIEFELPQPTEIIVRGIDKQKVGQVAANIRESRPPEPYKGK
                     GIRYRGEHVARKVGKRV"
     gene            complement(4700..5095)
                     /gene="rpsH"
                     /locus_tag="P5138_00980"
     CDS             complement(4700..5095)
                     /gene="rpsH"
                     /locus_tag="P5138_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRV59181.1"
                     /translation="MLTDPISDYLTRIRNGLSSGLATVEVPSSKLKLEVSRILTEQGY
                     ISGFEKSAADVGEKITVELKYTKDHQPVITGLERVSRPGRRRYVGHGEVPRVHGGTGT
                     AIITTSHGVMTGHEAKKEGVGGEVLAYVW"
     gene            complement(5100..5285)
                     /locus_tag="P5138_00985"
     CDS             complement(5100..5285)
                     /locus_tag="P5138_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRV59182.1"
                     /translation="MAKTSQKVRQQRKAKFSTREYNRCRRCGRPRAYYRKFGICRICL
                     REAAHEGYIPGMTKSSW"
     gene            complement(5555..5696)
                     /locus_tag="P5138_00990"
                     /pseudo
     CDS             complement(5555..5696)
                     /locus_tag="P5138_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
BASE COUNT          877 a         1847 c         1834 g         1138 t
ORIGIN      
        1 gagcggatga acttctcagc cacctcgtca agctcggcgg tggtcactcc gggccggacc
       61 ttggcggcca gcatcttcat gcagcgcgcg tggacggcgc ccgccgctgc catcttctcg
      121 atctgatcgt ccgtcttgcg gatgatcatc ggctgctgac tagctgcctt cttcgaggct
      181 gagggtcgaa acgagggtgc ggatcttctc gctcacccgg tcggggtcgt cggcgccttc
      241 gaccttgttg agcagcccct gcttcttgta atagtcgacc agcggcgcgg tcttctcctg
      301 atactgctcg agccgatgac ggaccacttc gggcttgtcg tcgtcacgga tgtagagctc
      361 ggagccgtcc acgtcgcaga tcccttcctg ctgcgggggg ttgtggtcga cgtggtagac
      421 gtggcccttc tcctgacaga cgcggcgtcc ggagaggcga ttgacgatct cctcgtcatc
      481 gacgtcgatc aggatcaccg cggtcaattg ccggccgagc tggccgagtt ccgcgtcgag
      541 cgcttcggcc tggaccactg tacgggggaa gccgtcaagg atgaacccgt gatcggcctc
      601 gccggactcg agccgctcgg cgatgatgcc gatgatcacc tcgtccggaa ccaggtcgcc
      661 ccggtccatg tactccttgg ctgccttgcc gacctcggtc ccttccttca cggcggcgcg
      721 caggatgtcg ccggtcgcgt agtacgggta ctcgaggtct tcctgcagcc gctcgccctg
      781 ggtgcccttg ccgctcccgg gcggtccgag caggatcaga ttgagttcgg ccacaggcag
      841 tcctctacct gaggaagcct tcgtagttcc gcatcatcag ctgcgcctcg agctgcttga
      901 ccgtgtcgag cgcaacgccc acgacgatca ggatcgaggt accgccaaag aacagaccac
      961 cgccgccgac gttattgaag aagatcgtcg gaaatgcggc cacggcaccg aggtagaggg
     1021 caccggggaa ggtcaggcgg gaaagcaccc ggtccaggta ctccgcggtc ggccggccgg
     1081 gacgcacgcc ggggatgaac ccgccgtact tcttcaggtt gtcggcctgg tccaccgggt
     1141 tgaaggtgac cgccgtgtag aagtaggtga agacgatgat gaagaacact tcaccgacca
     1201 cataggccca gctcgacggg ctcaggaatg cggcgaagtc gagtgccgcg ggggtgttga
     1261 tcagctggcc gatggtcggc ggcagcgcca tgatcgatgc ggcgaagatg accgggatga
     1321 cgccggccat gttgacgcgg agcggcaggt aggtggagcc gccggaggtc atcttgcgac
     1381 cgaccacgcg tttcgcgtat tgcaccggga tccggcgctg gccttcctgg acgaagacga
     1441 tcgcgacgat tacgccgagg acgatgaacg gcagcatcac gacgaagacc tgatccgggt
     1501 tgttccacca ggtctggatg cccggcagga ccccggaaac gatcgaggcg aagatcagca
     1561 gcgagatgcc gttgccgatg ccgcgctggg tgatcagctc gccgatccac atcagcaggg
     1621 tggtgccggc ggtgagacag atcacgatca gcaggacgtt ggccgggttc gtgttcgaga
     1681 cgacgctgtt cccgccggcc gacagcgagt tgaagaggaa gacgtaggcg aaggactgca
     1741 tcgcagccag cccgaccgtg aggtaacggg tgtactgcgt gatcttctgc tgcccgactt
     1801 cgccttcctt ctggaggcgc tccagcgagg gcacgaccac ggtcaggagc tgcaggatga
     1861 tcgaggccgt gatgtagggc atgatgccca gggcgaaaag tgacagtcgc gaaagcgagc
     1921 cgccggagaa caggttcagg aatccgagga tgttcgaacc ggtcagctgg ctttccagct
     1981 ccttgacggc atccacgtcg atgccgggcg ccgggatgta ggcgccgagg cggtacagcg
     2041 ccagcatggc ggctgcgaaa agaagcttcc tgcggatctc cgggacggag aacgctttcg
     2101 cgatcgtcgc gatcatcagt cgatcagctc gcagcttccg ccggcggcct cgatcttctc
     2161 cttggccgcg gccgagaagc cgtgtgcctt gacggtcagg ctcttggaga tctcaccacg
     2221 ggcgaggatc ttcaccggct cgttgcgggt cgccagaccg gcagccttga gcgattccgg
     2281 cgtcacttcg gcaccggaat cgaagcgatc ctcgagatcg cccaggttga ccggctgggt
     2341 gctggtccgg aagttctcga acggcatcga cttcttcatg tgcgggccgc gcagcttgcg
     2401 catccgcatg tgaatcgggg tctgcccgcc ttcgaggttc accttgcgct tgttgcccga
     2461 gcgctggccc tggccgccgt gtccgcggcc ggaggtcttg ccggtgcccg aaccgggtcc
     2521 gcggccgatc cgcttcttgg gacggcgcga gccgggcttg gggctcaggt tgtgaaggcc
     2581 gatttcctct tcgcggcttt cctgcttgtc agccatcagg cctcctccac cttgaccagg
     2641 tgggaaacca ccttgagctg accctggagc tggggtgagt catcgcgctc gaccgaacgg
     2701 ccgcgcttgc ccaggcccag ggcggcgagg gtgccacgct ggttcgggtt cgtgttgatt
     2761 tccgaacgaa tctgggtgat cgagagcttc gccatcaggc ctcttctcct tcggcggcag
     2821 ccttcttctc ggcgtcctcg gctacctctt cggcggcctg gacttcctcg gcgctttcgg
     2881 tcacttcttc cccgagatcg gcttcggcct tgacttcggc ctcgacttca gagctggacg
     2941 gctcggagcc atgatcggcg acggggcctt ccccggcggc gatgtcggct tccgccttct
     3001 cctcgacccc ggcgatgtcc gccggggttt cagcggcttc ctccgcaggc gcctcggtcg
     3061 cttccccggc cggagcttcc tccgccggtg cctcggccgg agcttcgtcg gcttcggtgt
     3121 cagcgtcgtg ggaaagggct tcctcggggg tcagatcggc ggcggcagat tcctggtcgc
     3181 ttgcagcttc cggagcggcg ccattaccgt tggcagtcgc ctcttcggcg gcagcctcgg
     3241 cctcggattc cttgtcgatg cccaggactt ccgtgatcgt caggccgcgc aactcggcga
     3301 cctcttccgg ccggcgcaga cggatgatgc cgtccatggt cgccttgacc aggttgatcg
     3361 ggttctgtgt gccgacgctc ttggtgagga tgtcgcgcac tccaccgagc tccagcacgg
     3421 cacggacgcc gccaccggcg ataacgccgg ttccagggct ggccgggcgc agcaccacgt
     3481 gaccggcgcc gtagcggccg atgatcttgt gggtgatcgt gtccccgtac ttggggatcc
     3541 gaaccaggtt cttgcgggcg ttctcgacgg ccttctggat cgcgagcgga acctcgcgtg
     3601 ccttgccgta gccgatcccg acgaccgact cttcatctcc aacagcaacg agggcagtga
     3661 acgagaaccg acgaccaccc ttgacgacct tggccacacg gttgatctcg atcacacgct
     3721 cctgcaggtc gagccccttc ggactgacca tctcaatgcg ggctgtatcc attcccttca
     3781 tcagctcttc accgtaccgg agttcctaga actccagtcc accctctctc gcaccttcgg
     3841 ccagggcctt gaccctgccg tggtagcggt aaccgcttcg gtcgaagaca caagtctcga
     3901 cgcctgcctt ctttgcacgt tctgccagca gctcgccggc cttcttcgcc tgatctgtgc
     3961 cgcggagctc cttgagcccg ggctcggtcc agttcgcggc ggccagcgtc ttgccatcga
     4021 cgtcgtcgat gagctgggcg aaaacaccac ggttggatcg gtaaacggac aggcgcggcc
     4081 gctgggcggt tcctgacacc ttggcccgga cccggtaccg gcggcgctga cggcgctgac
     4141 gcttggtaat gacggtcata ctcgcttacc gaccttcctg gcgacgtgtt cgccgcggta
     4201 acggatgccc ttgcccttgt aaggctccgg cggacggctc tcgcggatgt tcgccgcgac
     4261 ctggccgacc ttctgcttgt cgatccccct gacgatgatt tccgtcggct ggggaagttc
     4321 aaattcgatg ccttccggcg cctcgatcga gaccggatgc gaataaccga gcgcgagctc
     4381 gaggttcttg cccttggcgg cggcacggta gccgacgccc tggatctcga gcaccttctg
     4441 gtagccgtcg gtcacgccga cgatcatgtt cgcgatcagg ctgcgggtca ggccgtggag
     4501 cgcacgatgg ctgggccggt cggtcggccg ctcgatcgtg atcgtgccgt cctccgtctt
     4561 cacggtcatg tcggtcgaga tctcctgggt gagctcgccc ttcgggccct tgaccgtgat
     4621 cagtccgggc ttgatgtcaa tctcgacccc atcggggaga ctgatcggct ttcttccaat
     4681 tcgactcatc ggttcctcat caccagacgt aggcgagcac ttcgcccccg acgccttcct
     4741 tctttgcttc gtgaccggtc atcacgccat gcgaggtcgt gatgatcgcg gtgccggtgc
     4801 caccgtggac ccggggcacc tcgccgtggc cgacgtaacg acggcggccg ggacgggaaa
     4861 cccgctcaag cccggtgatg accggctgat ggtccttcgt gtacttgagc tcgaccgtga
     4921 tcttctcgcc cacgtcggca gcactcttct cgaagcccga gatgtagccc tgctcggtga
     4981 ggatgcggga gacctccagc ttcagcttcg aactgggcac ctcgacggtg gccaggccgg
     5041 agctgagacc gttgcggatg cgggtcaggt agtcggaaat cggatcggta agcatcagtt
     5101 caccagctcg acttcgtcat gccggggatg tagccctcat gggcggcctc gcgcaggcag
     5161 atccggcaga tgccgaactt ccggtaatac gcccgggggc gtccgcaccg gcggcaccgg
     5221 ttgtattcgc gggtcgaaaa cttcgccttg cgctgctggc gcaccttctg tgacgtcttg
     5281 gccattaggc ctggtcctcc tgcgtttctt cgtcggcggc ttcttcggcc tcttcggctg
     5341 ccgcatcttc agatgtgtct gctgcttcgg cttcagcctc ggcctcggca ccgtcatctg
     5401 actcttcagc cgatgcctct tccgagatct ccggagcttc ctcggccacg gcctcctcaa
     5461 ccggagcctc cggggttgct tcctcggcac cctcggggac cgggcggcct tccttcgcga
     5521 aaggcatgcc caggccgagc agcagctcga aagcctcgta gtccgtcgga gcggacgtgg
     5581 tgatcgtgat gtcgagacca cgggtctcgt cgaccgagtc gtaatcgatc tcggggaaaa
     5641 tcagctgttc gcggacgccc atcgaatagt tgccacggcc gtcgaacgag gtggcc
//
LOCUS       DAOTIP010000027         5646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_16974_length_5646_cov_3.752280, whole genome shotgun sequence.
ACCESSION   DAOTIP010000027 DAOTIP010000000
VERSION     DAOTIP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16974_length_5646_cov_3.752280"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="P5138_00995"
     CDS             complement(<1..1022)
                     /locus_tag="P5138_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HRV59183.1"
                     /translation="MTATDGAKGTVLVTGGSGFLGGRAIIDLLERGYEVRTTVRDKSK
                     GPRIEENISNEIGRPAGIEFFEANLDSDEGWPEAVSGCRYVLHIASPFPPNRPKDPQE
                     LIKPAVGGATRVINAALDAGVERVVMTSSVAAIRGGENQEDRDYTEDDWTNVDAVAAY
                     PQSKTLAEQAAWDLVEKRGEREKLATICPSAILGPVMPDDDSFSLEAIERLLNGMPAV
                     PEIGFSWVDVRDVSAAHIAAMEKPEAGGQRFIASGPFLWLLDVSKILREKLPEIAKKA
                     PTRKAPKFMVRITAIFDPGVRALIGDIGQRNDFDTTRAKEVLGVEARPIEETIVDCAA
                     SLAARS"
     gene            1046..1807
                     /locus_tag="P5138_01000"
     CDS             1046..1807
                     /locus_tag="P5138_01000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRV59184.1"
                     /translation="MRRLIDLSAPIEPGPEGAPEVVRVDVEYTPHEEGAKQIEALFGV
                     GPELLRDREGWAVDTFTLLGTHSSTHVDAPWHYNSTIQGKPAETIDQLPLDWFFRPGF
                     LLDFHERGDGEVIDVADIEAELDRIGYEVSERDICLVRTGCDQFLGTLEYMAHGPGVT
                     AGATRWLYDRGVRVMGIDAWGWDRPLWMQAEEAKEKGEAGIFWEAHQADIPYSQIERL
                     ANLEQLPPHGFTVACFPLKLVGGSAAPARVVAVLD"
     gene            complement(1815..3230)
                     /locus_tag="P5138_01005"
     CDS             complement(1815..3230)
                     /locus_tag="P5138_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59185.1"
                     /translation="MRSRLILLAVLALLVSALSPVGAGADVKGAAKRLDNLRDRPERL
                     RQFLRGMPKGTDLHTHLSGAVYAEAMIRWGAEDGKCVDTVTFASSFPPCTGDQVPLTA
                     ALTNTVLWDQILAAWSMREFRPDAFSGHNHFFATFDLFGSAFSGRQGDGLAQVTKRAA
                     TQNVQYIEPLITPQFGASLAIANQVGYNPDFATMRLAMLNAGITNAIPAAEQTATAAL
                     DRRSDLQGCGTDKPDPGCSVLVHFDVQVLRNQPPAVVFAQLLFGFELMKADPNWVGMN
                     MVQPEDAFYSTSQYNLHMRMVRYLRSVYPDAKVTLHAGELVPGLAPPSDLRFHIRQAV
                     NIAWADRIGHGADLRWERNPDQILRRMKRTGTCLEANLTSNQQILRLYGRRHPIRDYV
                     KAGVKVTLSTDDEGVSRTDLTSQYVKAVRQQGFGYRRLKQFARNGLRCAFLTPAEKKA
                     ALTDQATRFRKFEARFKSKQN"
     gene            complement(3251..4669)
                     /locus_tag="P5138_01010"
     CDS             complement(3251..4669)
                     /locus_tag="P5138_01010"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HRV59186.1"
                     /translation="MTSSSGPTTLTQDSPRLTGKELRKKAPRESHAEWKPAAGRDPLK
                     ILLKQAENRVQELLPIRYGRMSASPFAFYRGGAAIMAADLAPTPATGVRVQACGDAHI
                     SNFGGYRAPDRRVIFDLNDFDETLPAPWEWDVKRMAASAVIAARENGAGKKAARKIAL
                     AAVAQYRDVMRGLSEISYLDVWYSRLNMDDMADVLEQTHGEDAGIDLRRDISKAERKD
                     SSRAQRKLTEESDEGEPRFASRPPLLVPARELAESLGVTDADAVRQLLQEVLDQYAGT
                     LPPDRQHLFGHYRFVDIARKVVGVGSVGTRAWVLLFLSNRRMDPLVLQVKEAQASVLA
                     PYAGASKFRLQGRRVVEGQRLTQAASDLFLGWTRAKAPDGEIRDFYVRQLWDGKLSID
                     IPSLDESRLTGYVRACGGALALAHARTGDRVGIADYMGKNDSFPEAIAEFSIRYAKQN
                     KEDFKAFTAEIEAGRIEATPGI"
     gene            4829..5575
                     /locus_tag="P5138_01015"
     CDS             4829..5575
                     /locus_tag="P5138_01015"
                     /inference="COORDINATES: protein motif:HMM:NF018496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HRV59187.1"
                     /translation="MSGSGSTSDHLDIGGVFDRTGEIYQSAFGTFWLVALVLLVPAAV
                     IQWILGDGALGHFLGSVVQLIAGAWLAGSVVRIVQDVEASGDIDYTLGEFLGAVWPRL
                     LAIIVLQFLIGIMVAIGLFFLIIPGVIIALILAVSLPSLVVEDAGVFESMSRSAELTK
                     DNRMRILAVGILVILIVFGIAALVVLLGAITPLIGALVGLVLGILLYPYLAMLETVLY
                     FRLVELKEGGVVAVEETVVVEEDAGPPPAV"
BASE COUNT          903 a         1765 c         1896 g         1082 t
ORIGIN      
        1 tagctgcggg cggcgaggga cgcggcgcag tcgacgatcg tctcctcgat cggccgggcc
       61 tccacgccca ggacctcttt ggcccgggtc gtgtcgaagt cgttgcgctg accgatgtcg
      121 ccgatcaggg cccggacccc cgggtcgaag atcgccgtga tcctgaccat gaacttgggg
      181 gccttgcggg tcggggcctt cttggcgatt tccgggagct tctcgcgcag gattttcgac
      241 acgtcgagca gccagaggaa cggcccggag gcgatgaagc gctggccgcc ggcctcgggc
      301 ttctccatcg ccgcgatgtg ggcggccgac acgtcgcgga cgtccaccca gctgaatccg
      361 atctccggca ccgccggcat cccgttgagc agccgctcga ttgcctccag ggagaacgaa
      421 tcgtcgtcgg gcatgaccgg gccgaggatc gcgctggggc agatcgtcgc gagtttttcc
      481 cgttcgcccc gtttctcgac caggtcccag gcggcctgct cggccagcgt cttcgactgc
      541 gggtaggcgg ccaccgcatc cacattcgtc cagtcgtcct cggtgtagtc gcggtcctcc
      601 tgattctctc cgccacggat cgccgccacg gaggaagtca tgaccaccct ttcgacgccg
      661 gcgtcgaggg ccgcgttgat caccctggtc gcgccgccga ccgcgggctt gatcagctcc
      721 tgcgggtcct tcggcctgtt gggcgggaag ggggaggcga tgtggagcac gtatcggcaa
      781 cccgagaccg cttcgggcca gccttcgtcc gagtcgaggt tcgcctcgaa gaactcgatg
      841 ccggccgggc ggccgatctc gttcgagatg ttctcttcga tccgcggtcc cttcgacttg
      901 tcgcggaccg tggtccggac ctcgtaaccc cgttccagca gatcgatgat tgcccgtcca
      961 ccgaggaagc cggagcctcc ggtgaccagg accgtcccct tcgcaccatc agttgcggtc
     1021 atgtcagcag tctaggctga aaggcatgag gagattgatc gatctctcgg ctccgattga
     1081 gccgggaccc gaaggtgccc ctgaagtcgt gcgcgttgac gtcgagtaca cgccacatga
     1141 agaaggcgcg aagcagatcg aggcgctctt cggggtgggc cccgaacttc tcagggaccg
     1201 ggagggctgg gcggttgaca ctttcaccct gctcggcacc catagctcga cccacgtcga
     1261 tgcaccgtgg cattacaact cgacgattca gggcaagccg gccgagacga tcgatcagct
     1321 tccactcgac tggttcttcc ggcccgggtt cctgctcgat ttccacgagc gtggcgacgg
     1381 cgaggtgatc gatgtcgccg atatcgaggc tgaactcgac cggatcggct acgaggtgtc
     1441 agaacgggac atttgtctgg tcaggacagg ctgcgaccaa ttcctcggca cgctggagta
     1501 catggcccac ggccccgggg tcacggccgg agcaacccgg tggctctatg accgcggggt
     1561 gagggtgatg ggcatagacg cctggggctg ggaccggccg ctctggatgc aggcggagga
     1621 ggcgaaggag aaaggggagg cggggatctt ctgggaggcc catcaggccg acattcccta
     1681 ctcgcagatc gagcgcctcg cgaacctcga gcagctgccg ccgcacgggt tcacggtcgc
     1741 ctgtttcccg ctcaagctgg tcggcggcag tgccgcgcca gcccgggtgg tggccgtcct
     1801 ggactgaacc cgggtcagtt ctgcttcgac ttgaagcggg cttcgaattt cctgaacctt
     1861 gtggcctggt ccgtgagggc ggccttcttc tcggccgggg tgaggaaggc gcagcgcagc
     1921 ccgttgcggg cgaactgctt gagccgccgg tagccgaacc cctgctggcg gaccgccttg
     1981 acgtactggg aggtcaggtc ggtgcgggag acgccttcgt catcggttga aagggtcacc
     2041 ttgactccgg ccttcacgta atccctgatc ggatgccgcc tgccgtacag acggaggatc
     2101 tgctggttgg aggtcaggtt cgcctcgagg caggtgcccg tgcgcttcat cctcctcagg
     2161 atctggtccg ggttcctttc ccaccgaaga tccgctccgt ggccgatccg gtcagcccag
     2221 gcgatattga ctgcctggcg gatgtgaaac ctgaggtccg aaggcggggc gagcccgggg
     2281 accagctcac ccgcgtggag ggtcaccttc gcatccggat aaacggacct caggtagcgc
     2341 accattcgca tgtggaggtt gtattgggaa gtggaataga aggcgtcctc cggctgcacc
     2401 atgttcatcc cgacccagtt cggatccgcc ttcattagct cgaagccgaa gagaagctgg
     2461 gcgaagacca cggccggcgg ctggttccgg agaacctgga cgtcgaaatg aaccagcacc
     2521 gaacagcccg gatccggctt gtcggtgccg cagccctgca ggtcggaccg gcggtcgagt
     2581 gccgcggtcg cggtctgttc ggcggccggg atcgcgttgg tgattccggc gttcagcatc
     2641 gccaggcgca tggtcgcgaa gtccgggttg tagccgacct gattggcgat cgccaatgac
     2701 gcgccaaact gcggcgtgat cagtggctct atgtactgaa cgttctgggt ggctgcccgt
     2761 ttcgtaacct gggcgagccc gtcgccctgc ctgccgctga aggcggagcc gaaaaggtcg
     2821 aaggtggcaa agaagtggtt gtgaccggag aaggcatcgg gacggaactc ccgcatcgac
     2881 catgccgcaa ggatctggtc ccacagaacc gtgttggtca gggcggccgt caggggaacc
     2941 tggtcaccgg tgcagggcgg gaacgaggag gcgaaggtga cggtgtcgac acactttccg
     3001 tcctctgcgc cccagcggat catcgcttcg gcatagacgg cccccgaaag gtgcgtgtgg
     3061 aggtcggtcc ctttcggcat gccccgcagg aactggcgga gccgctccgg ccggtcgcgg
     3121 agattgtcga gccgctttgc cgcgcctttc acgtcggccc cggcccctac gggggacagg
     3181 gcgctgacca gcaacgccag cacggcgaga agtatcaggc gtgacctcac gacccgatcg
     3241 taagggaagc tcagattccc ggggtggctt cgatgcgtcc ggcctcgatc tcggcggtga
     3301 aggccttgaa gtcttccttg ttctgcttcg catagcggat gctgaattcg gcgattgcct
     3361 cggggaagct gtcgttcttg cccatgtagt cggcgatgcc gacccggtca ccggtccggg
     3421 cgtgggccag cgcaagcgcg ccgccgcagg cgcggacata tccggtgagc cttgactcgt
     3481 cgagcgaagg gatgtcgatc gaaagcttgc cgtcccagag ctgtcgcacg tagaagtcgc
     3541 ggatctcacc atctggcgct tttgctctcg tccagccgag gaaaaggtcg ctggccgcct
     3601 gggtaagccg ctgtccttcg accaccctgc gtccctggag cctgaatttg gaggcgccgg
     3661 catacggggc caggaccgaa gcctgggcct ccttgacctg aaggacgagt ggatccatcc
     3721 ggcggttcga aaggaagagc agcacccagg cccgggttcc gaccgagccg accccgacca
     3781 ctttccgggc gatgtccacg aagcggtagt ggccaaagag gtgctgccgg tcggggggca
     3841 gggtgccggc gtactggtca agcacctcct ggagcagttg tctcaccgcg tcggcgtcgg
     3901 tcacgccgag gctctccgcc agttccctgg cggggaccag aagcggtgga cgactggcga
     3961 agcggggttc gccctcatcg gattcctcgg tcagcttccg ctgcgcccgg ctgctgtcct
     4021 tgcgctccgc cttggagatg tcccggcgaa ggtcgattcc tgcgtcctcg ccatgagtct
     4081 gctcgaggac atcggccatg tcatccatgt tcaaccgcga gtaccagacg tcgaggtagc
     4141 tgatctccga gagtcccctc atcacgtccc ggtactgggc caccgcggca agtgcgatct
     4201 tccgtgccgc cttcttgccg gccccgttct cgcgtgcggc gatcacggcg ctggcagcca
     4261 tccgcttcac atcccactcc cagggggcgg gcagggtttc gtcgaagtca ttgagatcaa
     4321 agatcacccg gcggtccgga gccctgtagc cgccgaagtt cgaaatgtgg gcatccccgc
     4381 aggcctggac gcgaacgccg gtggcgggag tgggggccag gtcggcggcc atgatcgccg
     4441 cgcctcccct gtaaaaggcg aagggcgagg cggacatcct tccgtaccgg atcggcagga
     4501 gctcctgaac cctgttctct gcctgcttga gcaggatctt cagtggatcg cgaccggcag
     4561 ccggcttcca ttctgcgtgg ctctccctcg gtgccttctt ccgcagctcc ttcccggtaa
     4621 gtcggggtga atcctgggtc aacgtggtgg ggccggagga actggtcatg accgcagaat
     4681 attccccttg cggggacgcg attcattcaa tcgggatgaa agggaaaggg tcggatgaat
     4741 cggctttccc gcggggaggt atccggccga acacgtgcaa aggcgggtgt acgggctttc
     4801 ccgaatgcca ttccaaggag gagcaacaat gagcggttcc ggcagtacca gtgaccatct
     4861 cgacatcggc ggggtgttcg atcgaacagg tgagatctac cagagcgctt tcggcacttt
     4921 ctggctggtg gcattggttc tgctcgttcc ggccgcggtg atccagtgga tcctgggtga
     4981 cggtgctctt ggtcatttcc ttggttcagt ggttcagctg atcgccggcg catggctcgc
     5041 cggctcagtg gtccggatag tccaggacgt ggaagcgagc ggcgatatcg actacacgct
     5101 cggcgaattc ctcggtgccg tctggccccg gctgctggcc atcatcgtcc tgcagttcct
     5161 gatcggaatc atggtcgcca tcggtctgtt tttcctgatc atccccgggg tcatcattgc
     5221 gctgatcctc gcggtttcat tgccgtccct cgttgtcgag gacgcgggag tcttcgagtc
     5281 gatgtcgcgc agcgccgagc tgaccaagga caatcgcatg cgcatccttg cggtcggcat
     5341 cctggtgatc ctgatcgtct tcggtatcgc ggcgctcgtc gtgctgctcg gagcaatcac
     5401 tcccctgatc ggcgcccttg tcggcctggt tctcgggatc ctgctttacc cctaccttgc
     5461 gatgctcgag acggtccttt acttccgtct ggtggagctc aaggaaggcg gcgtggtcgc
     5521 ggtcgaagag accgtggttg tcgaggagga cgccggcccg ccgcccgccg tctaggcggc
     5581 ggcaaacttg cgcgggtccg ggtgggagcc cccggatcgg cgcacttcag gaaagggccg
     5641 cggctg
//
LOCUS       DAOTIP010000028         5631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17041_length_5631_cov_3.881815, whole genome shotgun sequence.
ACCESSION   DAOTIP010000028 DAOTIP010000000
VERSION     DAOTIP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17041_length_5631_cov_3.881815"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="P5138_01020"
     CDS             complement(<1..1091)
                     /locus_tag="P5138_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRV59188.1"
                     /translation="MIVDLKRRSFAYLLAGVVMLVAGLATVLLVVDDSQAATPGLEYG
                     QIAVDNPDGGTAATSSSPGSRIVGGSDTTFEKYPWQVQITGNGQPLCGGMLIHPMIVM
                     TAAHCLVDENLNFLPVTFALFTGRTQSGTGGEQLTLSGGAGVANNYVPSTHDNDYGFI
                     ALSSPSSRPRIQIAGADERGLWRAGRAAIVTGYGAIAEGGSQSPVLKHLAVPILDDST
                     CQGAGSYGAAFHSSVMLCAGYMSGGEDSCQGDSGGPLVAPADGGIYRLVGVVSWGVGC
                     ARPNLPGIYTRIAEPSLTQIIASYVQNIEQIENFPGQNNGISVVGSGAKPPGCSAANS
                     AAAAQQSKYNKANKAAAKARKALKKQQKKL"
     gene            complement(1189..2724)
                     /locus_tag="P5138_01025"
     CDS             complement(1189..2724)
                     /locus_tag="P5138_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59189.1"
                     /translation="MLLNFRISSRNSASFVLATALVSLVAILAAVMPLSSAGAAPIAS
                     VPPAPSLPDFSGKAFKAKPIKSATRAPQNPYMAKNQKSNIHNDTWMSGTYSWPGPLGR
                     NLVASSGSSANGICSTIAFDSRGRIVTVCVSTVAPPQARIVDADTLEVLDTYDLPDSA
                     AVDGTPAYQNFSAGGYFYLDNRNRLVIPTRNNHILLLGQTPDGSQFQLAGDYDLTSIV
                     KPNSERMNSALPDFQGDIWFVVKQSGKVGIIDRKTHRVRYIRLSETIQNSFTVDRGAV
                     YVVTNKRLYRLGKTKKDKPKVVWSAKYRNTGKKKPGQVDAGSGTTPDVMSGGYVAIVD
                     NANPENIVVYRTAKKLHGKKRKVCQVPVFKKGSSATENSLITTGRSMIVENNYGYTDI
                     FAPDAATVVTKPGFARVDIRKDGKGCRKIWTNWTERGASVVPKMSSKTGLIYTYTRPP
                     DPSGSQGYYWTAIDFRNGKTVWNKYAGSGFFHNNNYAGLGLGPDGTAYLGVVGGIIAL
                     RDS"
     gene            complement(2715..3809)
                     /locus_tag="P5138_01030"
     CDS             complement(2715..3809)
                     /locus_tag="P5138_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRV59190.1"
                     /translation="MKFLKTPAALCVLALAIVAMIAPTAASAGTASAGDPGASASVVG
                     GSNATGKHYPWQVVITANGQEFCGGVLIHPMVILTAAHCLVDEQGNYYEDYPGVTFRA
                     YAGRTNLDSGGEELNWKVARADPGYDPGTDTYDWGFVSLSSPASSHTLKLAGPNERAL
                     WKPGRMATVSGFGDLTDGGQGATILQQVNVPVLADSGCSKYGTAFYTSTMLCAGYLQG
                     GKDACQGDSGGPLSVTADHGVRRLIGIVSTGNGCALAGFPGIYSKVAASANSARIQSE
                     VESLESLDSFPSGYTGISVIGSGAKPLGCAAAVKSRNMASQKLKSLQRRLKAARRSGR
                     GNRFKAAQRRVKIARKKLGKSRARSRKACS"
     gene            complement(3900..5090)
                     /locus_tag="P5138_01035"
     CDS             complement(3900..5090)
                     /locus_tag="P5138_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV59191.1"
                     /translation="MSENGSSATTAAGASIPPPFDREHEDLRETVRRFVENEIAPHVD
                     EWEDARLFPRELFDRCAELGFLGLKFPEEYGGQGGSYLHDAIWVEELSRRGASGGVAA
                     GLNAHASIAMPPVFKFGNEWQKQRWLVPGIKGEKIGALGITEPGAGSDVASIRTMARK
                     VDGGYVVNGSKTFITNGVRADFLVCAVKTTEEGGHGGISFLVIETDSPGYEVVSKLEK
                     LGWHASDTGEISFTDVEVPEEHLLGEENEGFRLIMANFAWERLLMAIGAVGAMDALID
                     ASLEYANDRQAFGRSIGNFQAIRHKIAEMATTAAAARTLTYDTLRRFNEGEQVIREVS
                     MTKLMTQRALCEVADEAIQIHGGYGYMKEYGIERALRDARLGPIGGGTDEIMKDIIGK
                     TFGL"
     gene            complement(5083..>5631)
                     /locus_tag="P5138_01040"
     CDS             complement(5083..>5631)
                     /locus_tag="P5138_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV59192.1"
                     /translation="LVEAGKVRYLGLSEASAENIRRANAVHPISALQTEFSLFERGAT
                     DKVIPTLKELGIGFVPYSPLGRGFLTGRFRSGADFGSGDFREHDPRMSKDNLAANLAI
                     VEAIEELAAKHDVTSAQMAIAWVLAQSDDFVPIPGTKKVSRLEENAAATGISLSDEEL
                     AALDALPRPQGERYANMSSIDE"
BASE COUNT          893 a         1791 c         1845 g         1102 t
ORIGIN      
        1 agcttcttct gctgtttctt cagggccttc ctggccttcg ccgctgcctt gttcgccttg
       61 ttgtatttcg actgctgggc ggcggcagcg gagttggcgg ccgaacagcc cggaggcttc
      121 gcgccggacc ccacaacgct gatgccgttg ttctggcccg ggaagttctc gatctgctcg
      181 atgttctgga cataggacgc gatgatctgg gtcaatgagg gctccgcgat cctcgtgtag
      241 atgcccggca ggttcggacg ggcacatccg actccccagc tcacgacccc gaccaggcgg
      301 tagatgccac cgtcggctgg tgcgacgagc ggtccgccgc tgtcgccctg acaggagtcc
      361 tcaccgccgc tcatgtagcc ggcgcagagc atcaccgatg aatggaacgc cgccccgtat
      421 gagcccgctc cctggcatgt ggagtcgtcg aggatcggta ccgccaggtg cttgaggacg
      481 ggactctggg agccaccttc cgcgattgct ccataaccgg taacgattgc ggccctcccg
      541 gccctccaca gcccccgttc gtcagcgccg gcgatctgaa tccgcggcct tgaagacgga
      601 ctcgacaggg cgatgaagcc gtagtcgttg tcatgggtcg agggaacgta gttgttggcc
      661 acgcccgcac cgccgctgag cgtcagttgc tctcctccgg taccgctctg cgtccgcccg
      721 gtgaacaggg cgaaggtgac cggcaggaag ttcaggttct cgtccacaag gcagtgagcc
      781 gcggtcatca cgatcatcgg atgaatcagc atgccgccgc acaagggctg gccgttgccg
      841 gtgatctgga cctgccaggg gtacttctcg aaggtggtgt cactgccacc gacgatccgg
      901 ctgcccgggc tcgaggaggt tgcagccgtg cctccgtccg ggttgtcgac cgcgatctgg
      961 ccgtactcga ggcccggcgt ggccgcctgc gaatcatcga caacgaggag aacagtcgcc
     1021 aggcccgcga ccagcatcac caccccagcc agcaagtagg cgaagctgcg cctcttcagg
     1081 tcgacgatca tgaattaccc cctgtggtag ttctttcgta cctgctttcg acgctaacac
     1141 atgccgcagc gactcgcaga agccggccgg tggccggccc gggtcagctc aggagtccct
     1201 gagggcgatt attccgccga ccactccgag gtaggcggtt ccgtccggac cgaggccgag
     1261 gccggcgtag ttgttgttgt ggaagaagcc cgaaccggcg tacttgttcc agaccgtctt
     1321 tccgttccgg aaatcgatcg cggtccagta gtagccctgg gagccgctcg ggtcgggcgg
     1381 ccgggtgtac gtgtagatca acccggtctt cgatgacatc ttcggcacga ccgaagctcc
     1441 gcgttcggtc cagttcgtcc agatcttccg gcagcccttg ccgtccttgc ggatgtcgac
     1501 ccgggcgaaa ccgggcttgg tgaccacggt cgcggcgtcg ggggcgaaga tgtccgtgta
     1561 gccgtaattg ttctcgacga tcatcgaccg gccggtcgtg atcagcgaat tctcggtagc
     1621 gctggacccc ttcttgaaga cgggcacctg gcagaccttg cgcttcttgc cgtggagttt
     1681 cttcgctgtg cggtacacga cgatgttctc cgggttcgcg ttgtcgacga tcgccacata
     1741 gccgccggac atcacatccg gagttgttcc gcttcccgcg tcgacctgtc cgggcttctt
     1801 cttgccggtg ttccggtact tggcgctcca gacgaccttc ggcttgtcct tcttcgtctt
     1861 gccaagccgg tagaggcgct tgttggtcac cacgtagacc gctccgcggt cgacggtgaa
     1921 cgaattctgg atcgtctcgc tgagccggat gtaacgcacc cggtgggtct tgcggtcgat
     1981 gatcccgacc ttgcccgact gtttgaccac aaaccagatg tctccctgga aatcgggcag
     2041 ggccgaattc atgcgctccg aattcggctt gacgatcgag gtcaggtcgt agtcgccggc
     2101 cagctggaac tgcgagccgt ccggggtctg gccgagcagg aggatgtggt tgttcctggt
     2161 cgggataacc aggcggttcc ggttgtccag gtagaagtag ccgccggccg agaagttctg
     2221 gtaggcgggc gtgccgtcga ccgccgccga gtcgggcagg tcataggtgt cgagcacctc
     2281 gagcgtgtcg gcatcgacga tccgcgcctg cggcggtgcc accgtggaaa cgcaaacggt
     2341 gacgatccgg ccgcggctgt cgaaggcgat ggtcgagcag atgccgttgg ccgaggaccc
     2401 ggaggaggcg accaggttcc tgccgagcgg tcccggccag ctgtaggtgc cgctcatcca
     2461 ggtatcgttg tggatgttgc tcttctggtt cttcgccatg taggggttct gtggtgccct
     2521 ggtggcgctc ttgatcggct tggccttgaa ggccttgccc gagaagtccg gaagcgacgg
     2581 tgcgggcggg accgaggcga tcggtgccgc gccggctgac gaaagcggca tcacggcagc
     2641 gagaatcgcg accagggata cgagcgccgt ggcaaggacg aacgaagcgg agttcctgct
     2701 gctgatccgg aaattcaaga gcatgccttt ctcgatcttg cccggctctt cccgagcttc
     2761 ttcctggcaa ttttgacccg ccgctgggca gctttgaacc ggttacctct ccccgacctg
     2821 cgtgcggcct tgagcctgcg ctgcagggac tttagcttct ggctggccat gtttctggac
     2881 ttgaccgcgg cggcgcagcc gagcggcttc gccccggagc cgatcacgct gatgcccgtg
     2941 tagcccgaag ggaacgaatc gagcgattcc aggctctcga cctcggactg gattctggcg
     3001 ctgttggcgg aagcggcaac cttggagtag atgcccggga atccggccag ggcgcaacca
     3061 tttcctgtgc ttacgatccc gatcagccgc cggacgccgt ggtccgcggt gacgctgaga
     3121 ggaccgccgc tgtcgccctg gcaggcgtct tttccaccct gcaggtatcc ggcacagagc
     3181 atggtcgagg tgtagaaggc tgttccgtac ttcgagcaac ccgagtccgc gaggacgggc
     3241 acattcacct gctgcaggat cgtggccccc tgacccccgt cggtcaggtc accgaagccg
     3301 ctgacggttg ccatcctccc gggcttccac agtgcccgtt cgttcggacc ggcgagcttg
     3361 agggtgtggg aggaagcagg gctcgaaagg cttacgaagc cccagtcgta ggtgtccgtt
     3421 cccggatcgt agcccgggtc ggctcgcgcg accttccagt tcagctcctc gcccccggag
     3481 tcgagattgg tgcggccggc gtaggcccgg aaggtaacgc cgggatagtc ctcgtaatag
     3541 ttgccctgtt catccaccag gcaatgggcg gcggtgagga taaccatcgg gtggatcagg
     3601 acgccaccgc agaactcctg gccgttggcg gtgatcacga cctgccacgg gtagtgcttg
     3661 ccggtggcgt tgctgcctcc gacaaccgag gcggaggcac ccggatcacc ggctgaagcg
     3721 gttccggccg acgcggcggt gggggcgatc atcgcgacga tcgccagggc gagcacgcaa
     3781 agcgctgccg gtgtcttcag gaatttcaag tctttctccg ctttggtcgg ccggttcgtg
     3841 ggggcgagcc gctcgccagg cgcctgcagg tggcccgggg ctcaccgaat agaacccggc
     3901 tagaggccaa aggtcttgcc gatgatgtct ttcattatct cgtcggttcc gccgccgatc
     3961 ggccccagcc tcgcgtcgcg cagggcccgt tcgatcccgt actccttcat gtagccgtaa
     4021 ccgccgtgga tctggatggc ctcgtcggcc acttcgcaga gggcgcgctg ggtcatcagc
     4081 ttggtcatcg agacttcgcg aattacctgt tctccctcgt tgaagcgtcg cagggtgtcg
     4141 taggtgaggg tcctggcggc ggcggccgtg gtggccattt cggcaatctt gtggcggatc
     4201 gcctggaagt tgccgatcga gcggccgaag gcctggcggt cgtttgcgta ctccagcgag
     4261 gcgtcgatga gggcatccat cgccccgacg gccccgatcg ccatcaacag gcgctcccag
     4321 gcgaagttgg ccatgatcag ccggaagccc tcgttttcct cgccgagaag gtgctcttcg
     4381 ggaacttcga cgtcggtgaa ggaaatctcc cccgtatcgg aggcatgcca gccgagcttc
     4441 tccagcttcg agacgacctc gtatccgggc gagtcggttt cgatgaccag gaaggagatt
     4501 ccgccgtggc cgccttcctc ggtcgtcttc accgcgcaga ccaggaagtc cgcccggaca
     4561 ccgttggtga tgaaggtctt ggacccgttg accacatagc ctccgtcgac cttccgggcc
     4621 atggtccgga tcgaggcgac atccgagccg gcaccgggct cggtgatgcc cagcgcgccg
     4681 atcttctctc cctttattcc ggggaccagc cagcgctgct tctgccactc gtttccgaat
     4741 ttgaagaccg gcggcatggc gatggaggcg tgggcgttca ggccggccgc gaccccgccg
     4801 gaggctcccc tgcgggaaag ttcctccacc cagatcgcgt catgaaggta gctgccgccc
     4861 tgaccgccgt actcctccgg gaacttgagc ccgaggaacc cgagctctgc gcagcgatca
     4921 aagagctccc gcgggaacag gcgggcgtcc tcccattcat ccacatgcgg tgcgatctcg
     4981 ttctcgacga agcgccggac cgtttcccgc agatcttcat gctcgcgatc gaaagggggc
     5041 ggaatcgagg ctccggcggc ggtggtcgcc gaagagccgt tttcactcat cgatcgagct
     5101 catgttggcg taccgctcac cctgcggacg gggcagggcg tccagcgcgg caagctcctc
     5161 gtcgctcagg ctgatcccgg tcgccgcggc gttctcttcc aggcggctca ccttcttggt
     5221 cccggggatc ggcacgaagt cgtcactctg ggccagcacc caggcgatcg ccatctgggc
     5281 cgaggtgacg tcgtgtttgg cagccagttc ctcgatcgcc tcgacgatcg caaggttggc
     5341 cgccaggttg tccttgctca ttcgtgggtc atgctcgcgg aagtccccac ttccgaagtc
     5401 ggccccggaa cggaagcggc cggtcaggaa gccacggccg agcggcgagt aggggacgaa
     5461 gccgatcccc agctccttca gggtggggat caccttgtcg gtggcgccgc gctcgaagag
     5521 cgagaactcg gtctgaaggg ccgaaatcgg gtgaactgcg ttggccctgc ggatgttctc
     5581 ggccgaggcc tccgagagcc cgaggtagcg gaccttgccg gcctcgacca g
//
LOCUS       DAOTIP010000029         5556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17353_length_5556_cov_3.667515, whole genome shotgun sequence.
ACCESSION   DAOTIP010000029 DAOTIP010000000
VERSION     DAOTIP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17353_length_5556_cov_3.667515"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..596
                     /locus_tag="P5138_01045"
     CDS             48..596
                     /locus_tag="P5138_01045"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRV59193.1"
                     /translation="MDEATMKVHHDKHHQAYVDKANAALEGTEWADKDVAEVVANLSS
                     LPADKQGPVRNNAGGHYNHSLFWEMMSPDGGGAPSGDLAAAIDAAFGSFDDFKEQFKA
                     GGIGQFGSGWVWLVKDGDGVAIVSTPNQDSPLTDGKTPLLGADVWEHAYYLKYQNKRP
                     DYLDAFWNVVNWDYVAERYAAA"
     gene            695..1720
                     /locus_tag="P5138_01050"
     CDS             695..1720
                     /locus_tag="P5138_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRV59194.1"
                     /translation="MIGLPTRTAVLPLPPKQIFFLALLAFAGLGMLISGCGSSDSGDE
                     VARSAPSASDFPSAEGKTIEQLLNDQTPSDKVVAPAAVVFEPGKNRFPFGVFNVDRSQ
                     VEDADVALYFAKSPQSPVEGPFPAEVVSLETKPAYRSKGAGGPGEANSFYLANVKFDR
                     AGPWLGLAILKVDGDTEAARITPSPVVSQYPNVAKVGQKAPKVSTPTASDVGGDLSKI
                     DTRQPPSSMHTEDLKDVLGKKPVVLVFATPALCQSRVCGPVVDVAEQVKDEVGDGVVF
                     IHNEVYNDNDPSKGIRPQLTAFGLQTEPWIFLINKDGTIEKRIEGAIGVDELKADVEQ
                     LKKKNGL"
     gene            complement(1733..3445)
                     /locus_tag="P5138_01055"
     CDS             complement(1733..3445)
                     /locus_tag="P5138_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRV59195.1"
                     /translation="MDHDYDWVIVGSGFGGSVSALRLAEKGYKVAVIEVGRRFEDKDF
                     ARTAWNMRRYFWLPKFGLRGILRMSMFKDIFVVSGCGVGGGSLGYANTLYRPRPAFYT
                     DPQWDSLADWEVELGPHYVTAERMLGVTLYRDTGPADALLHEYADEIGADKTYSNTQV
                     GVFFGEPDKTVSDPYFGGEGPDRTGCNKCGSCMVGCRYGAKNTLVKNYLYFAEKLGVK
                     VFPGREVTSVRPIGPADGSEGYELMTDRSGAVLRHRRKKITAGAVIFSAGALGTNKLL
                     ANCAHNGDLPNLSERIGHVVRTNSESIQAVTARDDERDFSDSVAITSSIYPDPDTHIE
                     VVTYGQAGDVISRLYTIYTGNGTRLTRPIRFLAAMARRPLDSLRLLVAPRHWSRRTVI
                     LLVMQALDSAMRLKPVKKKFGKGVRLQTEQDPERPNPTFIQAAEDATNWFAKRTGGIP
                     QVSFGESLFNIPTTAHILGGAVVGHSPEDGVVDKDNHVFGYENLMVCDGSAVPANPGV
                     NPSLTITAMTERAMSKIPPKDPEAEPLHLPDEARPARTADNTPPASTPGPSGTAESWT
                     PLEP"
     gene            3691..5430
                     /locus_tag="P5138_01060"
     CDS             3691..5430
                     /locus_tag="P5138_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59196.1"
                     /translation="MKQRLGVIVGVIAAFLVGTFAMYGTASAAGDASSSATKTKQQKK
                     LESNVKSAKKKANKTCKKVKKAKGKKAKKQAKKRCKKAKNNVKKQQKKLNQYKAAQYF
                     DVCKHGCKYRTVQKGADAAGTWQEKTKRKATVRVQPGTYVEGVFLDGRNPNYNYNGLT
                     IMGVTSKKKPASNPKGVILEGENAKTIVDGNPQVANNAIEGRSIIGLVMKNMWARHYQ
                     NNTFFVWAGHDGTERCADYTMDNLLSTDTRSYGFFARNCYGGTIENSEGYHHGDSALY
                     VGETPCDSEDWTNRTHAADRTPCQANPDWTVIKNVKSHANVLGYSGTNSKYVEIKDSA
                     FYNNGAGVVPNTLDSEKFEPSGWLKIHDNDIFWNNYNYYSTGSAFQTVATIGDVNYPI
                     GIGVVLYGTDSVQVYDNNIFGNEKWGAATFSGPELFDVNEGSDAMNMNNQFVDNNMGR
                     DGVDPNGNYDFFSDYSGGSNCWQGNTAGSTFAPGNGSVPVSTIYPDSCPQPVVGKLDV
                     QAVELNAGLQINWGSYKDDGDPSNGPGDADTIFGYSTQHPPQRQECSMTVNTPHPAYM
                     DYVEDRADPVNCL"
BASE COUNT         1090 a         1784 c         1747 g          935 t
ORIGIN      
        1 gaggtgctcg tacccccaag ctgagccccc tggcactcga gccgcacatc gacgaggcga
       61 ccatgaaggt gcaccatgac aagcaccacc aggcctacgt cgacaaggcc aacgccgccc
      121 tcgaaggcac cgaatgggcc gacaaggacg tggccgaagt cgtcgccaac ctttcctcac
      181 tgcccgccga caagcagggc ccggtccgca acaacgccgg tggccactac aaccactccc
      241 tgttctggga gatgatgagc cccgacggcg gaggcgcccc gtccggtgac ctggccgccg
      301 ctatcgacgc tgccttcggc tcgttcgacg acttcaagga gcagttcaag gccggtggaa
      361 ttggccagtt cggttccggc tgggtctggc tggtcaagga cggcgacggt gtcgcgatcg
      421 tctcgacccc gaaccaggat tcgccgctca ccgacggcaa gaccccgctg ctgggcgccg
      481 acgtctggga gcacgcctac tacctcaagt accagaacaa gcgcccggat tacctggacg
      541 ccttctggaa cgtggtcaac tgggattacg tggccgagcg ttacgccgcg gcctagggcc
      601 gaagccccga cgaagttgaa gggccgtgat gcgcctcgcg catcacggcc cttttatgtt
      661 ccggtgaggc gtgtttctgt agcctgatca atcgatgatc ggccttccca ccaggaccgc
      721 ggtcctgccc cttcccccga agcagatctt tttcctcgcc ctgcttgcct ttgccgggct
      781 cggcatgctg atctccggtt gcggctcgag cgactccggc gacgaggtcg cccggagcgc
      841 cccttcggcc tccgacttcc cgtcggccga aggcaagacg atcgagcagc tcctgaacga
      901 ccagacgccc agcgacaagg tcgtggctcc ggccgcggtg gtcttcgaac ccgggaagaa
      961 ccgcttcccg ttcggggtgt tcaacgtcga ccgcagccag gtcgaggatg ccgacgtcgc
     1021 gctctacttc gccaagagcc cccagtcacc ggttgagggt ccgttcccgg ccgaggtcgt
     1081 gtcgctcgag accaagccgg cttaccgctc caagggtgcc ggcggcccgg gcgaggcgaa
     1141 cagcttctac ctggccaatg tcaaattcga ccgggcggga ccgtggctcg gcctggcgat
     1201 cctcaaggtc gatggtgaca ccgaagcagc ccggatcact ccgagcccgg tggtcagtca
     1261 gtacccgaac gtggcgaagg tcggccagaa ggccccgaag gtcagcaccc cgaccgcctc
     1321 ggacgtcggt ggcgaccttt ccaagatcga cacccggcag ccgccttcga gcatgcacac
     1381 cgaagacctg aaggacgtgc tgggcaagaa gccggtcgtg ctcgtcttcg cgaccccggc
     1441 actctgccag tcccgcgtct gcggaccggt ggtggacgtg gccgaacagg tcaaggatga
     1501 agtcggcgac ggcgtcgtct tcatccacaa cgaggtctac aacgacaacg atcccagcaa
     1561 gggcatccgc ccgcagctga ctgcgttcgg gctccagacc gagccgtgga tcttcctgat
     1621 caacaaggac gggacgatcg aaaagcggat cgagggcgcg atcggcgtcg acgagctgaa
     1681 ggcagacgtc gagcagctca agaagaagaa cggcctctag gccgccccgg gatcagggct
     1741 ccagcggagt ccaggactcg gcggtcccgg aaggtccggg tgtgctggcc ggcggggtgt
     1801 tgtccgcggt ccgggccggc cgggcctcgt ccggcaggtg aagcggctcg gcctccgggt
     1861 ccttcggcgg gatcttgctc atcgcccgct cggtcatcgc ggtgatggtc agactcgggt
     1921 tgacgcccgg gttggccggc acggccgagc catcacagac catcaggttc tcgtagccga
     1981 atacatggtt gtccttgtcg accacgccat cttccggact gtggccgacc acggcgccgc
     2041 cgaggatgtg ggcggtggtc gggatgttga agagcgactc tccgaaggag acctgcggga
     2101 tgccgccggt ccgtttcgcg aaccagttgg tcgcgtcttc ggccgcctgg atgaaggtcg
     2161 ggttgggtcg ctccgggtcc tgttcggtct ggagacgcac gcccttgccg aatttcttct
     2221 tgaccggctt gagccgcatc gccgaatcga gcgcctgcat gaccagcagg atcaccgtcc
     2281 gcctcgacca gtggcggggt gccacgagca ggcgaagcga gtccagcgga cgccgcgcca
     2341 tcgctgccag gaacctgatc ggccgggtga gcctggtccc gttgccggtg tagatcgtgt
     2401 agaggcggct gatcacgtcg ccggcctgcc cgtatgtgac cacctcgatg tgggtgtcgg
     2461 ggtccgggta gatgctggag gtgatcgcca ccgagtcgct gaagtcgcgc tcgtcgtcgc
     2521 gggcggtgac cgcctggatc gactccgaat tggtgcggac cacgtggccg atccgctccg
     2581 agagattcgg caggtctccg ttatgggcgc agttggccag cagcttgttg gtgccgagcg
     2641 ccccggccga gaagatcacg gcgccggcgg tgatcttctt tcgccggtgg cgcagcaccg
     2701 ccccggagcg atcggtcatg agttcgtagc cctccgatcc gtcggccggg ccgatcggcc
     2761 tgaccgaggt gacctcgcgg ccggggaaga ccttgacccc gagcttctcg gcgaagtaga
     2821 ggtagttctt gaccagggtg ttcttcgccc cgtaccggca gccgaccatg caggagccgc
     2881 atttgttgca gccggtccgg tccgggcctt cgcccccgaa gtacggatcc gaaacggtct
     2941 tgtcaggctc gccgaagaag acgccgacct gggtgttcga gtaggtcttg tcggcaccga
     3001 tctcgtccgc gtattcgtgg agcagggcat cggccggtcc ggtgtcccgg tagagggtga
     3061 cgcccagcat ccgttccgcg gtcacgtaat gaggaccgag ctcgacttcc cagtcggcca
     3121 ggctgtccca ctgcgggtcg gtgtagaagg cggggcgggg acggtagaga gtgttcgcgt
     3181 aaccgaggct gcctccgccg accccgcagc cggacaccac gaagatgtcc ttgaacatgg
     3241 acatgcggag gatcccccgc agcccgaact tcggcagcca gaagtatctg cgcatgttcc
     3301 aggccgtcct ggcgaagtcc ttgtcctcga agcggcggcc gacctcgatc accgccacct
     3361 tgtagccctt ctcggccagc ctcagggcgg agacgctgcc gccgaatccg gagccgacga
     3421 tcacccagtc gtagtcgtga tccatgagtt cattctacct ttcccatacg cctgaccggt
     3481 ttgtttggga ttttctttcg gattcccgat cggcgcaaac cctgatcaga ggctagattc
     3541 cgcgccagac cttgatttca cccgatttgg atgagggccg ggaccgaaac cttttcctaa
     3601 aaggtggttt agagtgtccg gatggctttc ccgcgctcct gcgggggcca gcaacccttt
     3661 ggcattcaac aaaactttcc aaggagagag atgaagcaac gtttgggagt aatcgtcggt
     3721 gtcattgccg cgttcctggt gggaacgttc gcaatgtacg ggacggcgtc ggcagcaggt
     3781 gacgccagtt ccagcgcgac caagacgaag cagcagaaga agcttgagag caacgtcaag
     3841 tcggccaaga agaaggccaa caagacttgc aagaaggtca agaaagccaa gggcaagaag
     3901 gccaagaagc aggccaagaa gcgctgcaag aaggcgaaga acaacgtcaa gaagcagcag
     3961 aagaagctga accagtacaa ggctgctcag tacttcgacg tctgcaagca cggctgcaag
     4021 taccgcaccg tccagaaggg cgctgacgcc gccggcacct ggcaggagaa gaccaagcgc
     4081 aaggctacgg tccgcgtcca gccgggcacc tacgtcgagg gcgttttcct cgatggccgt
     4141 aacccgaact acaactacaa cggcctgacc atcatgggcg tcaccagcaa gaagaagccg
     4201 gcctcgaatc cgaagggcgt catcctcgag ggtgagaacg ccaagacgat cgtcgacggc
     4261 aacccccagg tggccaacaa cgcgattgaa ggccgcagca tcatcggcct cgtgatgaag
     4321 aacatgtggg cccggcacta ccagaacaac acgttcttcg tgtgggccgg tcacgacggc
     4381 actgagcgtt gtgccgacta cacgatggac aacctgctgt cgaccgacac ccgttcctac
     4441 ggcttcttcg cccggaactg ttacggcggc accatcgaga actccgaggg ctaccaccac
     4501 ggtgactctg ccctctacgt cggtgagacc ccctgtgact ccgaagactg gaccaaccgg
     4561 acccacgcgg ccgaccgcac tccgtgccag gccaatccgg actggacggt catcaagaac
     4621 gtcaagagcc acgccaacgt tctcggttac tcgggtacca actcgaagta cgtcgaaatc
     4681 aaggactcgg ccttctacaa caacggtgcc ggcgtcgtgc cgaacaccct cgactcggag
     4741 aagttcgagc cgagcggctg gctgaagatc catgacaacg acatcttctg gaacaactac
     4801 aactactact cgaccgggtc tgctttccag accgtcgcca ccatcggtga cgtcaactac
     4861 ccgatcggca tcggcgtcgt cctgtacggc accgacagcg tccaggtcta cgacaacaac
     4921 atcttcggca acgagaagtg gggcgcagcc accttctccg gtccggaact gttcgacgtc
     4981 aacgaaggca gcgacgcgat gaacatgaac aaccagttcg tcgacaacaa catgggtcgc
     5041 gacggcgtgg atccgaacgg aaactacgat ttcttctcgg actactccgg aggcagcaac
     5101 tgctggcagg gcaacaccgc cggctccacg ttcgcccccg gcaacggctc ggttccggtc
     5161 agcaccatct acccggacag ctgcccgcag cccgtggtcg gaaagctgga cgtccaggca
     5221 gtcgagctga acgccggcct gcagatcaac tggggttcct acaaagacga cggtgacccg
     5281 tcgaacgggc ctggggatgc ggacacgatc ttcgggtact cgacgcagca tccgccgcag
     5341 aggcaggagt gctcgatgac tgtcaacacc ccgcacccgg cgtacatgga ttacgtcgag
     5401 gatcgcgccg acccggtgaa ctgcctctag gcgcagttca gcccccaacc aattcaaggc
     5461 aggaaggtaa gaatcaagat gagtaagaag ttcatgaaaa gagcagccgc atggctgtcc
     5521 gtgggaatcg ttgcgctggc gctgggggcc gccggc
//
LOCUS       DAOTIP010000030         5513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17526_length_5513_cov_3.631000, whole genome shotgun sequence.
ACCESSION   DAOTIP010000030 DAOTIP010000000
VERSION     DAOTIP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17526_length_5513_cov_3.631000"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="P5138_01065"
     CDS             complement(<1..635)
                     /locus_tag="P5138_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRV59197.1"
                     /translation="MAERIPRDQSLSRIYGASALFAAAYGNVGSSIYYALGVTAAFAL
                     GLTPVAFMIAGLIFICTAATYAEATVMYPEAGGSSSFGRHAFNELVSFVAAWAQMLNY
                     TITVAISAYFVPHYLAVFWPALGHAPGDIVGGAVVIALLAWLNVRGGQESARLNIILA
                     VADLATQVVLVAIGMFLVFNTNVLIENIHLGVAPTWPDFALGIAVGMIAYTG"
     gene            complement(647..1111)
                     /locus_tag="P5138_01070"
     CDS             complement(647..1111)
                     /locus_tag="P5138_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59198.1"
                     /translation="MGSDSEHADSGAVDDLRASVGRVSLEVQALADRVQELVAAVESL
                     APRRATAPVAPASPSPVEGELVVSVSPLPELAMAAVAETALRGLSVVRKVVSVERSNA
                     QATFVLEVDEGGELIAEMRRAMPVPITVDTAPDGSLNVVLEWAWGRSTTSAG"
     gene            complement(1217..2797)
                     /locus_tag="P5138_01075"
     CDS             complement(1217..2797)
                     /locus_tag="P5138_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59199.1"
                     /translation="MLVGLGVSLAAMPAASGAAAKKKRVGPCSSLPINIKLSSHDPTG
                     LLEDGYAWIYVNPKGSVRSAMVRVMRGNRTYARGRIVGKMAPGRTTLIRMHVNRRMTQ
                     GKYRVKVTASKPGRCSRRVSKVRPWHFSTPSLGLKAIPVSTRIGDNVDVVRFALRPVR
                     RTKVGWTRVSLVGPGGSVISQQVIKSIGSKQILAELPIQGKLKAAKYRVRLTGQDLAT
                     GNFRTSTQSWVFGKGGGGAKPVPTTGQLTQKVAVDWTKGKWEGRQVGGFIAPGIGYGE
                     VVCSPYQQWVRFYPSDTSRESAMMTWTYKNWDFGKGTEKAVREAKFADGTGPDFREGM
                     NKFGPTEKHSTGTFQGIISDRGPLDGPGGATLASPTTFDLDWVWDFNKSGRSYCHVKA
                     TFRTQTDQVTDPLARSVQIVWRGEANATAANTVSDVDFPDLGNVQAICQAGPNGYRRL
                     IVDSAIGGTVYTREGSDDFAVNQNTGPLVMRLPNNGMLFIQMNNGDRILVSSRWKVND
                     PVASNNWCVVSAQIYSAS"
     gene            complement(2908..4338)
                     /locus_tag="P5138_01080"
     CDS             complement(2908..4338)
                     /locus_tag="P5138_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV59200.1"
                     /translation="MRQVLKRKGTGVAFASAVLVLLVTGCGVNPDEAPQSAGHTGADA
                     QYVPPQTGQKQVKERRATTRETGTIRVAGKAQGSLTPDLVKAYRAAGGTTAVTIQDET
                     ENRAFRQFCAGRADVVDSARPISASEYRQCIANGIQPVQFQVASDAAVLAIKNETDVG
                     TDCLSFDEVRNIFRAGSTINSWSQVGYGHDLGGDSNEPRMKVAGPDENSNVFGFFGQY
                     VLGDPEPTMLSVRSDYESFPTDAGVRRAVVGSNKDFKAAAQYVRSRQTYQSILDSLSE
                     NRRAVRDARAEVAKGIADGRTAAAKARDRRKLAEAKKNLENLINSIPEAKRYLAQNRA
                     ALRRLAEVRGTLGLFRFSYYEAFEEQLRPMEISASNDFANPECIFPSQATVTNATYPL
                     ARQLLLTINYKNMTDTDLNDFLVQSLSRSQQLAENAALVPLPDDTQATELAWLNGSTE
                     PDIIFYESGSGTTVGTKQQADSASGQ"
     gene            complement(4335..>5513)
                     /locus_tag="P5138_01085"
     CDS             complement(4335..>5513)
                     /locus_tag="P5138_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59201.1"
                     /translation="APVDPVVELEELTGTTGTTSSTANATTGQTQPGTGSSVTANSDP
                     VASGRLFSADSPWNTSVEGLPAADNSDRMLELATQRRAAKEVPGQEGVVTFTRQIVRD
                     GIFINTEAWAPLVVAANTNDSVETKFVCRQSKCGSNNPKVPGSLNIPANTTPDPRFDG
                     WLSVIDRQNGMGYDFWRARREGDGTISYQFGKAWTLDGPGFSKPVSVDAERAVGARGS
                     GLPLFAGLIGPKELEAGSINHALAISVPGLARRNYVQPASVTDGVGNRKSIPAGARIR
                     LRSGVRINEKKLQTLKGQRRRSADAILAALRTYGAIVVDRSAVPSLYAPLGTSSNLIR
                     GDEVDWLKPEDFEVISLPQIYKDPPLSQVGLEGPATGGNNSQVQSGSAASGNEVGGGL
                     "
BASE COUNT          933 a         1763 c         1799 g         1018 t
ORIGIN      
        1 cccgtgtagg cgatcattcc gaccgcgatg cccagcgcga aatcaggcca ggtcggagcc
       61 acaccgaggt ggatgttctc gatcaggacg ttggtgttga agaccaggaa catgccgatc
      121 gcgaccagca ctacctgggt tgcgaggtcg gccaccgcga ggatgatgtt gagccgggcc
      181 gactcctggc caccgcgcac gttcagccag gcgagcagtg cgatcaccac cgcgcctccg
      241 acgatgtcgc cgggcgcatg acccagggca ggccagaaca cggccagata atgggggacg
      301 aagtaggccg agatggcgac cgtgatcgtg tagttcagca tctgcgccca ggcggcgacg
      361 aagctgacga gctcgttgaa ggcatgccgg ccgaacgagg aggaaccgcc ggcctccggg
      421 tacatgaccg ttgcttccgc ataggtcgcg gcggtacaga tgaagatcag gccggcgatc
      481 atgaacgcga ccggggtgag tcccagcgcg aacgcggcgg tgaccccgag cgcgtagtag
      541 atcgacgagc ccacgttgcc gtaggcagcg gcgaaaagag cgcttgcgcc gtagatccgg
      601 ctcaggctct gatcacgcgg gattcgttca gccaagtcgc atcagctcag ccggcactgg
      661 tggtggacct tccccaggcc cactccagga ctacgttcag cgagccatcg ggggcggtat
      721 caaccgtgat cggaaccggc attgcccggc gcatctcggc gatcagctcc cccccttcat
      781 cgacttccag gacgaatgtc gcctgggcgt tcgaacgctc gaccgaaacc accttgcgca
      841 cgacgctgag tccgcgaagc gcggtctcgg cgacggcggc catcgccagt tcgggcagcg
      901 ggctcacgct gaccaccagc tcgccctcca ccgggctcgg cgaagcgggc gccaccggcg
      961 ccgtggcgcg ccggggagca agagattcaa cggcggcgac cagctcctgt acccggtcgg
     1021 ccagggcctg cacttccagg cttaccctgc ctaccgatgc cctgaggtcg tcaacggcgc
     1081 cactgtcagc gtgttccgag tctgaaccca cgcgctcata ctaactgcat gccatccggt
     1141 ctttgccgga tgacaacaga aaattcccgg cccgcaggat tcagggacgg acgggccggc
     1201 ttgggaagga ttttcctcag gaggcgctgt agatctgggc ggagacgacg caccagttgt
     1261 tcgaggcaac cgggtcgttc accttccatc gggaggagac caggatgcgg tcgccgttgt
     1321 tcatctggat gaagagcatg ccgttgttgg gcagccgcat caccagaggt ccggtgttct
     1381 ggttgaccgc aaagtcatcc gaaccttcac gggtgtagac cgtgcctccg atcgccgagt
     1441 cgacgatcag tcggcggtag ccgttggggc ccgcctggca gatcgcctgg acgttgccaa
     1501 gatcggggaa gtcgacgtcg ctcaccgtat tggccgcagt ggcattggcc tcaccgcgcc
     1561 agacgatctg gaccgaacgc gcaaggggat cggtgacctg gtcggtctgg gtacggaagg
     1621 tcgccttgac gtggcagtac gaccgccccg acttgttgaa gtcccacacc cagtcgaggt
     1681 cgaaggtcgt cggcgaggcc agcgtggcac ccccgggccc gtcgagcggg ccccggtcgg
     1741 aaatgatccc ctggaaggtc ccggtcgagt gcttctcggt cggcccgaac ttgttcatgc
     1801 cttcgcggaa gtcgggtccg gtaccgtcgg cgaacttggc ttcacgaacc gccttctcgg
     1861 tgcccttgcc gaaatcccag ttcttgtagg tccaggtcat catcgcgctc tcccgactgg
     1921 tgtcggaggg gtagaaccgg acccactgct ggtaagggct gcagaccact tcgccgtagc
     1981 cgatcccggg cgcgatgaag ccgcccacct gccgtccttc ccacttgccc ttcgtccagt
     2041 cgaccgcgac cttctgggtc agctggccgg tggtcggaac aggcttggcg ccacccccgc
     2101 ccttgccgaa gacccagctc tgggtggaag tgcggaaatt gcctgtggcc agatcctgac
     2161 cggtcaggcg cacgcggtac ttcgcagctt tcagcttccc ctgtatcggg agctcggcga
     2221 ggatctgctt gctgccgatt gacttgatca cctgctggct gatcacgctg ccgccgggcc
     2281 cgaccagaga aacgcgagtc cagccaactt tggtgcggcg gaccggacgc agtgcaaacc
     2341 ggaccacgtc gacgttgtca ccgatgcgtg tactcaccgg aatcgccttc agaccgaggc
     2401 tcggcgtcga gaaatgccat ggcctgacct tgctcaccct ccggctgcac cgaccgggct
     2461 tgctggccgt gaccttgacc ctgtacttgc cctgagtcat ccgccggttc acatgcatcc
     2521 ggatcagggt cgtgcgaccg ggtgccatct tgccgacgat tcgtccgcgg gcataggttc
     2581 ggttgccgcg catgaccctg accatcgccg agcgaaccga tcccttgggg ttgacgtaga
     2641 tccaggcata cccgtcctca agcagtccgg tcggatcatg ggagctcaac ttgatgttga
     2701 tcggaagcga cgagcaggga cccactcgct tcttcttggc cgccgcgccc gaggcagccg
     2761 gcatcgcggc cagggaaaca cccagaccga cgagcaacag ggctgtagct atgcgtcgaa
     2821 acactgaatt catgatctca ccccgcgtta aaccgctgtt caagggcaag tagcggcgaa
     2881 gaggccagat tcgggcggtc ctggaaacta ctggccactg gccgaatcgg cctgctgctt
     2941 ggtcccgaca gtggtgccgg agccggactc atagaagatg atgtcgggct cagtgcttcc
     3001 gttgagccag gccagctccg tggcctgggt atcgtccgga agcgggacca gggcggcgtt
     3061 ttcggcgagc tgctgcgaac gggaaaggct ctggaccagg aaatcgttga ggtccgtatc
     3121 cgtcatgttc ttgtagttga tcgtgagcag gagctggcgg gccagcgggt aggtcgcgtt
     3181 ggtgacggtc gcctgcgagg ggaagatgca ctccgggttc gcgaagtcgt tcgaggcgct
     3241 gatctccatc gggcggagct gctcttcgaa ggcctcgtag taggaaaacc ggaagagccc
     3301 gagcgtgccg cgaacctcgg ccagtcgacg cagcgccgcc cggttctggg cgaggtagcg
     3361 cttggcctcc ggaattgaat tgatcaggtt ctcgaggttc ttcttggcct ctgcgagctt
     3421 gcggcgatcg cgtgccttgg ccgcggccgt gcgcccgtcg gcgattccct tggcaacctc
     3481 ggcccgggcg tccctcaccg cacggcggtt ctcggacagc gaatcgagga tgctctggta
     3541 ggtctggcgc gaacgcacgt actgtgccgc cgccttgaag tccttgttgc tgccgaccac
     3601 ggcgcggcga acgccggcgt cggtcgggaa cgactcgtag tcgctccgga ccgacagcat
     3661 cgtcggctcg gggtcgccga gcacgtattg accaaagaag ccgaagacgt tcgaattctc
     3721 atccggtccg gcgaccttca tccgcggttc attcgaatcg ccgccgagat catgtccgta
     3781 tccgacctgg ctccagctgt tgatcgtcga cccggcccgg aagatgttcc tgacctcgtc
     3841 gaagctgagg cagtcagtgc cgacgtcggt ctcgttcttg attgccagaa cggcggcgtc
     3901 cgaggccacc tggaactgga ccggctgaat gccgttggcg atgcactggc ggtactcgga
     3961 agcggagatc ggacgggccg aatcgaccac gtcggcgcga cccgcgcaga actggcggaa
     4021 ggcccggttc tcggtctcgt cctggatggt caccgcggtg gtaccgccgg ctgcccggta
     4081 agccttgacc aggtccgggg tcagcgatcc ctgcgccttg ccggccaccc ggatggttcc
     4141 ggtttcacgc gtggtcgccc ttctttcctt tacctgcttc tggccggtct gcgggggcac
     4201 gtactgggcg tcggcgcccg tatggccggc cgactgtggt gcttcatccg gatttacgcc
     4261 gcaacccgtg accagaagga ccagaaccgc agaggcgaag gcgacgcccg tgcccttcct
     4321 tttcagaacc tgcctcacag tccacctccg acttcgttgc cagacgcagc gctgccggac
     4381 tggacctgcg aattgttccc gccggttgcg ggcccttcaa ggccgacttg gctcagcggc
     4441 gggtccttgt agatctgcgg cagtgagatg acttcgaaat cttcgggctt gagccagtcg
     4501 acttcatcgc cacggatcag gttggaggaa gttcccagtg gtgcgtacag gctgggcacc
     4561 gcggaccggt cgaccacgat cgccccgtaa gtgcgaaggg ccgcgagaat cgcgtcggcc
     4621 gaacgccgcc tctgaccctt cagggtctgc agcttctttt cgttgatcct cacgccgctg
     4681 cgcagtctga tgcgggcgcc cgccgggatc gacttgcggt tgccgacgcc atccgtgacc
     4741 gaagcgggct gcacgtagtt gcggcgggca agaccgggaa ccgagatcgc cagggcatgg
     4801 ttgatcgagc cggcctccag ttccttcggc ccgatcagcc ctgcgaagag tggcagtccg
     4861 gatccccggg ctccgacggc ccgctccgca tcgacgctga ccggcttgct gaagcccggc
     4921 ccgtcaagag tccaggcctt gccgaactgg tacgagattg tgccgtcgcc ttcccgccgg
     4981 gcccgccaga agtcatagcc catgccgttc tggcggtcga tgacgctgag ccagccgtcg
     5041 aatctcggat cgggggtcgt attggccgga atgttcaggc tgccggggac cttcggattg
     5101 tttgaaccgc atttcgactg gcggcagacg aacttggttt ccacggaatc gtttgtgttg
     5161 gccgccacaa caagaggcgc ccaggcctcg gtgttgatga agatcccgtc gcggacgatc
     5221 tggcgggtga aggtgaccac gccttcctga ccgggaactt ccttcgccgc ccggcgctgg
     5281 gttgcaagct cgagcatgcg atccgagtta tcggctgccg gcaggccttc gacggaagta
     5341 ttccagggac tgtctgccga gaagagccgg cccgacgcga ccggatcgga gttcgcggtg
     5401 accgaggatc ccgtgcccgg ctgggtctgt cccgttgtcg cgttggcggt cgatgacgtg
     5461 gttcctgtcg tgcccgtcaa ttcctccaac tcgaccaccg ggtcgactgg tgc
//
LOCUS       DAOTIP010000031         5499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17579_length_5499_cov_3.387032, whole genome shotgun sequence.
ACCESSION   DAOTIP010000031 DAOTIP010000000
VERSION     DAOTIP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17579_length_5499_cov_3.387032"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /gene="rplD"
                     /locus_tag="P5138_01090"
     CDS             <1..623
                     /gene="rplD"
                     /locus_tag="P5138_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRV59202.1"
                     /translation="TKADLPAGLFDEAFHQSLVHETARADANARRQGTASTLTRGEVS
                     MTTAKAWRQKGTGRARVGALSVPNRYGGGAAFGPKPRHYTVKVNRKARRKAMRSALSV
                     HAERGSVAVVKAADFETPSTKDASAALDKWGAKRSTLVVVLAEETGILKSFRNIPRVS
                     VLEVGATGVVDVIGAASIVISEGALEVLAKQSTEPARGSETEKDGE"
     gene            623..916
                     /gene="rplW"
                     /locus_tag="P5138_01095"
     CDS             623..916
                     /gene="rplW"
                     /locus_tag="P5138_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRV59203.1"
                     /translation="MDPRSVIIRPVISEKAYALIAAGKYTFRVDDRAHKFEIKDAVEK
                     AFGVEVVKVRTSTVRSKPKRRGLHSGRSRSWKKAIVELAPGDTIELFEGAAVE"
     gene            937..1782
                     /gene="rplB"
                     /locus_tag="P5138_01100"
     CDS             937..1782
                     /gene="rplB"
                     /locus_tag="P5138_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRV59204.1"
                     /translation="MAIRKTKPTSPGRRFATYQLREQITASEPEKSLVEGIKKSGGRN
                     SNGRITSRHRGGGAKRRYRKIDFKRTKDGIPAKVATVEYDPNRSAYIALLHYADGEKS
                     YILAPSQIEVGNTVSSGPGSDIRPGNCLALGEMPVGTVVHNVELKAGQGGKLGRSAGT
                     AIQLVAKEGDMVTLRLPSSEMRMVRAECRATIGTLSNAEHQNVKIGKAGRNRHKGKRP
                     QTRGIAMNPVDHPHGGGEAHKTPGGHPVTPWGKPTLGYRTRKKGKQSDRYIVRGRRRG
                     KKKGR"
     gene            1786..2052
                     /gene="rpsS"
                     /locus_tag="P5138_01105"
     CDS             1786..2052
                     /gene="rpsS"
                     /locus_tag="P5138_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRV59205.1"
                     /translation="MGRSTKKGPYVEERLMSRINSMNEAGNKNMVKTWSRRSTIFPEM
                     VGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRTFKSHTGGKD"
     gene            2052..2435
                     /gene="rplV"
                     /locus_tag="P5138_01110"
     CDS             2052..2435
                     /gene="rplV"
                     /locus_tag="P5138_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRV59206.1"
                     /translation="MAARTRKKEEAAEPVLVRATSKYVRVSPRKARLFADQVRGKHID
                     DARSLLQFSPRSAAEDVSKVIESAAANAEANHDLIGDEMIIAEIRVDEGPTLKRFRPR
                     AMGRATPIHKRTCHISVALTPEDEE"
     gene            2443..3162
                     /gene="rpsC"
                     /locus_tag="P5138_01115"
     CDS             2443..3162
                     /gene="rpsC"
                     /locus_tag="P5138_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRV59207.1"
                     /translation="MGQKIHPEGFRVGYIHDWKSNWFADKEFSDLLMQDLAIRDHIEN
                     KLAHAGLSDITIERRGEVAVDIHTARPGIVIGKSGSEVDALRKDLHKITGSPVKVNIR
                     EVKRPELDAKLVAQSIAEQLQNRVAFRRAMKRALTSAMRSGAKGVKVQISGRLGGAEM
                     ARTEGYSDGRVPLHTLRADIDYGFVEAKTGTGRIGVKCWINKGEVMPEGFESSRTSDE
                     PPASDRPDRRGGRDDRRGGRR"
     gene            3162..3581
                     /gene="rplP"
                     /locus_tag="P5138_01120"
     CDS             3162..3581
                     /gene="rplP"
                     /locus_tag="P5138_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRV59208.1"
                     /translation="MLMPKRVKHRKVMRGRRKGNSKGGTAVTFGEYGLKSLERGWITN
                     RQIEAARVAMTRKIKRGGKVWINVFPDKPVTKKPAETRMGSGKGNPEAWVAVVKPGRV
                     MFELSGVPEELAREAMRVAGHKLPVKTKFVQREGAGE"
     gene            3578..3793
                     /gene="rpmC"
                     /locus_tag="P5138_01125"
     CDS             3578..3793
                     /gene="rpmC"
                     /locus_tag="P5138_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRV59209.1"
                     /translation="MKSAEAHSMSDVELVEKLKEVREESFNLRLRHATGELEDTASLG
                     RAKKDLARLLTIAHQRNIDLERESKHK"
     gene            4196..4423
                     /gene="rpsQ"
                     /locus_tag="P5138_01130"
                     /pseudo
     CDS             4196..4423
                     /gene="rpsQ"
                     /locus_tag="P5138_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            4427..4795
                     /gene="rplN"
                     /locus_tag="P5138_01135"
     CDS             4427..4795
                     /gene="rplN"
                     /locus_tag="P5138_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRV59210.1"
                     /translation="MIQQESRLRVADNSGARELLCIRVKGGSHRKYGSIGDVITATVK
                     QASPQGNVKKGEVVQAVIVRTKKELGRSDGTYIAFDENAAVLIDAQNNPRGTRIFGPV
                     ARELRDRDFMKIISLAPEVL"
     gene            4795..5121
                     /gene="rplX"
                     /locus_tag="P5138_01140"
     CDS             4795..5121
                     /gene="rplX"
                     /locus_tag="P5138_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRV59211.1"
                     /translation="MRIRTDDQVQVISGKEKGKQGKVLRTDPKAGTVYVEDLNMIKRH
                     QRAQPSPDGRGQGKEGGIIEMEGPIHVSNVALVDPTDNKPTRIGIRTTDDGKRQRYSK
                     RTGEAI"
     gene            5130..>5499
                     /gene="rplE"
                     /locus_tag="P5138_01145"
     CDS             5130..>5499
                     /gene="rplE"
                     /locus_tag="P5138_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRV59212.1"
                     /translation="MEAATTTQQAVPPPRLRDTYNDEVLPKLMETFGYSTPMRAPRLE
                     KITLNMGLGSSVDSKSREKAIGELAQIAGQMPNTRIAKKSIASFKLREGMPVGASVTL
                     RGARMWEFLDRLCSIAIPRIR"
BASE COUNT         1136 a         1852 c         1761 g          750 t
ORIGIN      
        1 caaccaaggc cgatctgccg gccggcctct tcgacgaggc cttccaccag tcgctggtcc
       61 acgagaccgc ccgcgccgac gccaacgcgc gtcgccaggg cactgcttcg accctgaccc
      121 gcggcgaggt ttcgatgacc accgccaagg cctggcgcca gaagggcacc ggtcgtgccc
      181 gcgttggtgc cctcagcgtg ccgaaccgtt acggcggcgg tgcggccttc ggcccgaagc
      241 cgcgtcacta cacggtcaag gtcaaccgca aggcccgccg caaggcgatg cgttcggccc
      301 tctcggttca cgccgagcgt ggcagcgtcg ccgtggtcaa ggcggccgac ttcgagaccc
      361 cctcgaccaa ggacgcctcg gcggccctgg acaagtgggg cgccaagcgc tcgaccctgg
      421 tcgtcgtgct ggccgaggag acgggaatcc tcaagagctt ccgcaacatc ccccgggtca
      481 gcgtgctcga ggtgggtgcg accggagtcg tcgacgtgat cggcgccgct tcgatcgtga
      541 tttccgaagg cgccctcgag gtgctggcaa agcagtccac cgaaccggcc cgcggttccg
      601 agaccgaaaa ggatggtgag tgatggatcc ccgcagcgtg atcatccgtc cggtcatttc
      661 cgagaaggca tacgccctga tcgcggccgg caagtacacc ttccgggttg acgaccgggc
      721 ccacaagttc gagatcaagg acgccgtcga aaaggcgttc ggagtcgaag tggtcaaggt
      781 ccggacctcc acggtccgtt ccaagccgaa gcgccgcggc ctgcattcgg gccggagccg
      841 ctcctggaag aaggcaatcg tcgaactcgc ccccggcgac accatcgagc tgttcgaagg
      901 cgcggcggtc gagtaagggc ttagaggaat caagtaatgg caattcgcaa gacaaaaccg
      961 acaagccccg ggcgccgctt cgcgacgtac cagctgcgcg agcagatcac ggcctcagag
     1021 ccggagaaga gcctggtcga gggcatcaag aagtccggtg gccgcaactc gaacggccgc
     1081 atcacctccc gccatcgtgg cggaggggcc aagcgccgct accggaaaat cgatttcaag
     1141 cggaccaagg acgggatccc cgccaaggtc gcgacggtcg aatacgaccc gaaccgctcc
     1201 gcctacatcg ccctgcttca ttacgcggac ggtgagaaga gctacatcct cgccccctcg
     1261 cagatcgagg tcggcaacac ggtcagctcc ggccccggct cggacattcg tcccggcaac
     1321 tgcctcgccc tcggcgagat gccggtcggc accgtggtcc acaacgtgga gctgaaggcc
     1381 ggccagggcg gaaagctcgg ccgcagcgcc ggcaccgcga tccagctggt cgcgaaggag
     1441 ggtgacatgg tcaccctgcg ccttccgtcc tccgaaatgc gcatggtccg cgcggaatgc
     1501 cgcgccacca tcggcacgct ctccaacgcc gagcaccaga acgtcaagat cggcaaggcc
     1561 ggccgcaacc gtcacaaggg caagcgcccg cagacccgcg gtatcgccat gaacccggtc
     1621 gaccacccgc atggcggtgg cgaggcccac aagacccccg gcggccatcc ggtcaccccg
     1681 tggggcaagc ccacgctcgg gtaccgaacc cgcaagaagg gcaaacagtc cgatcgttac
     1741 atcgtgcgcg gccgtcgtcg cggaaagaag aagggtaggt agaccgtggg taggtcaacc
     1801 aagaagggcc cgtacgtgga agagcggctg atgagccgga tcaattccat gaacgaggcc
     1861 ggtaacaaga acatggtcaa gacctggtca cgtcgctcga cgatcttccc cgagatggtc
     1921 ggccacacga ttgccgtcca tgacggccgc aagcacgtgc ccgtcttcat ctccgagtcg
     1981 atggtcggtc acaagctggg cgagttcgcc ccgacccgta ccttcaagtc gcataccggc
     2041 ggtaaggact gatggccgcc aggaccagga agaaggaaga ggcggccgag ccggtgctgg
     2101 ttcgtgccac ctccaagtac gtgcgcgtgt cgccccgcaa ggcccgcctg ttcgccgatc
     2161 aggtgcgtgg caagcacatc gacgacgccc gctcgctgct gcagttttcc ccgcgttcgg
     2221 ctgccgagga cgtctcgaag gtgatcgagt cggcagccgc caacgccgag gccaaccacg
     2281 acctgatcgg tgacgagatg atcatcgccg agatccgggt tgacgaagga cccaccctga
     2341 agcggttccg tccgcgggcg atgggccggg ccaccccgat tcacaagaga acttgccaca
     2401 tctccgtggc gctgacaccg gaagacgagg aatagaagac ctatgggaca gaagatccat
     2461 cccgaaggct tccgcgtcgg ctacatccac gactggaagt ccaactggtt cgccgacaag
     2521 gagttttcgg acctgctgat gcaggacctg gcgatccgcg accacatcga gaacaagctt
     2581 gctcacgccg gcctgtccga catcacgatc gaacgtcgcg gcgaggtcgc cgttgacatt
     2641 cacaccgccc gtccgggcat cgtgatcggc aagtcgggca gcgaggtcga cgcgcttcgc
     2701 aaggaccttc acaagatcac cggctcgccg gtgaaggtga acatccgcga ggtcaagcga
     2761 cccgaactgg acgcgaagct ggtcgcccag tcgatcgccg agcagctcca gaaccgcgtc
     2821 gccttccgcc gcgcgatgaa gcgtgccctg acctccgcca tgcgctccgg cgccaagggc
     2881 gtcaaggtcc agatctccgg ccgcctgggc ggtgccgaga tggcccgcac cgagggttac
     2941 tcggacggcc gtgtgccgct ccacaccctg cgcgccgaca tcgactacgg attcgtcgag
     3001 gccaagaccg gcaccggccg catcggcgtc aagtgctgga tcaacaaggg cgaggtcatg
     3061 cccgagggct tcgagtcctc ccgcacctct gacgagccgc ccgccagcga ccgtcccgac
     3121 cgccgcggcg gccgtgatga ccgcagggga ggtcgtcgct gatgttgatg ccgaagcggg
     3181 tcaaacaccg caaggtcatg cgtggccgcc gcaagggcaa ctcgaagggc ggcaccgccg
     3241 tgaccttcgg cgagtacggg ctcaagtccc tcgagcgcgg ctggatcacc aaccgccaga
     3301 tcgaggctgc ccgtgtcgcg atgacccgaa agatcaagcg tggcggcaag gtgtggatca
     3361 acgtcttccc ggacaagccg gtcaccaaga aaccggccga aacccgcatg ggttccggca
     3421 agggcaaccc ggaagcctgg gtcgccgtgg tcaagcccgg ccgcgtgatg ttcgagcttt
     3481 ccggcgtgcc ggaggaactg gcccgtgaag cgatgcgcgt cgccggccac aagcttccgg
     3541 tcaagaccaa gttcgtccag cgggaaggag ccggagagtg aagtccgccg aagcccacag
     3601 catgagcgac gtcgagctgg tcgagaagct caaggaggtc cgcgaggaat ccttcaacct
     3661 gcgcctccgt cacgccaccg gcgaactcga ggacacggcc tctctcggcc gagccaagaa
     3721 ggatctcgcc cggctgctga ccatcgccca ccagcgcaat atcgacctcg agcgcgagtc
     3781 gaagcacaag taggaacaaa tgtcagaaga agaaactgaa aacacagaag tagaagaagc
     3841 cccggccgag gaagctgccg aagctccggt cgaagaggcc cctgctgaag aggctcccgc
     3901 cgccgaggaa gcgccggctg aagaggctgc gcccgcagcc gaagaaccag ccgaacccga
     3961 tccgctcgac gagcttccct ggaaggaacg ccgtcgggtg ctcaagtcgc gtgaatccgg
     4021 cgaggccggc cccgagcgca gcggtgagca gcgtcaggcc gaccgtgacg tcacccgggc
     4081 gaagaaggcg gccgtccgtc gcaaccgccg ggccgaggtc aagtccgccc ggaagggcga
     4141 cggcgtcggc accccgcggg ccgagaagac ccgtaccggc aagccgaagg agcgtcaggg
     4201 caccgtcgtt tccgacaaga ccgacaagac catcacggtc cagatcgaca ccgcccgccg
     4261 tcacccggtc tacgagaaga tcgtccgtca ctcgcacaag atccacgcgc atgacgagaa
     4321 caacgacgcc cacgagggcg acgtggtccg cgtgatcgag acccgcaagc tttccaagac
     4381 caagcactgg cgcctggtcg aggtcgtgga gaaggcgagg tagcaggtga tccagcagga
     4441 atccagactc cgcgtcgccg acaactccgg cgcccgcgaa ctcctctgca tccgcgtcaa
     4501 gggcggctcg caccgcaagt acggctcgat cggtgacgtg atcactgcga ccgtcaagca
     4561 ggcgagcccg cagggcaacg tcaagaaggg cgaggtcgtc caggccgtca tcgtgcggac
     4621 caagaaggag ctcggacgct ccgacggcac ctacatcgcc ttcgacgaga acgctgccgt
     4681 gctgatcgac gcccagaaca acccgcgtgg cacccgcatc ttcggaccgg ttgcccgcga
     4741 gctgcgcgac cgtgacttca tgaagatcat ttcgctggcc ccggaggtgc tgtgatgcgt
     4801 atccgtaccg acgaccaggt tcaggtgatc agcggcaagg aaaagggcaa gcagggcaag
     4861 gtcctgagga ccgacccgaa ggccgggacc gtgtacgttg aagacctcaa catgatcaag
     4921 cgccaccagc gcgcccagcc ttcgcccgac gggcgcggcc agggcaagga aggcgggatc
     4981 atcgagatgg agggtccgat ccacgtttcc aacgtggcac tggtcgaccc gactgacaac
     5041 aagccgaccc gcatcggcat ccgcaccacc gacgacggca agcgccagcg ctattcgaag
     5101 cgcaccggcg aggccattta gagagcaaga tggaagcagc aacgacaaca cagcaggcag
     5161 tgccgccgcc gcggctccgg gacacctaca acgatgaggt tctcccgaag ctgatggaga
     5221 ctttcggcta ttcgaccccg atgcgcgcac cgcgcctcga gaagatcacg ctgaacatgg
     5281 gcctcggctc gtcggttgac tccaagtcgc gcgaaaaggc gatcggcgaa ctcgcccaga
     5341 tcgccggcca gatgccgaac acccgcatcg ccaagaagtc gatcgcttcc ttcaagctgc
     5401 gcgaaggtat gccggtcggt gcctcggtga ccctgcgcgg agcccggatg tgggagttcc
     5461 tggaccggct ctgctcgatc gcgatcccgc ggatccgtg
//
LOCUS       DAOTIP010000032         5489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17617_length_5489_cov_3.375414, whole genome shotgun sequence.
ACCESSION   DAOTIP010000032 DAOTIP010000000
VERSION     DAOTIP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17617_length_5489_cov_3.375414"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="P5138_01150"
     CDS             <1..275
                     /locus_tag="P5138_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59213.1"
                     /translation="YTRGKWRPYRTGQWSWSYVNIVGLVPTFYDIYGQGHYWRTGTGG
                     TIFTPFNGLRAGYYAVYQVAYWAASKKKRTKFTPIYPDARSYCTFV"
     gene            complement(272..1666)
                     /locus_tag="P5138_01155"
     CDS             complement(272..1666)
                     /locus_tag="P5138_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV59214.1"
                     /translation="MVPHLIAAEWLPFQGLDPFFQALFMITFLIVVAMFFWTIVLFFR
                     GVRSTREMVHRDEVNPELFEWVFLVPALNEEVTIADSVSRLEALEIRRKRIVVINDGS
                     DDRTAEILAERTDPDLYVVERKPPEARQGKAAALNFAFHEVTSRFKFNPETTIFCVVD
                     ADGRIAPDSLPFVAQHFMESEVGGVQTLVRIYNRHQVLTWFQDLEFSIYGHLFQAGRN
                     KWGTAGMGGNGQYNRMDALISIDDRDPGPGEEAIVEGDPEPDVETDRVSRGPWRDRLT
                     EDQDLGLRLLVAGWRCHHDNRATVEQQGLPGLRRLFRQRTRWSQGNLQAMGLIDDMVT
                     SKLYFPARFEQVVYLMMPVWQMIVGASLIASIYLFFFEGISFFANASDWWWLYFLYLL
                     GFGGTVLGSIAAKIEDRNILLGIIKGLLTGQIYAFYTWLLWPVLFRSSIRQFTSRDTW
                     AKTSREQIHPAADS"
     gene            complement(1651..3051)
                     /gene="wecB"
                     /locus_tag="P5138_01160"
     CDS             complement(1651..3051)
                     /gene="wecB"
                     /locus_tag="P5138_01160"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRV59215.1"
                     /translation="MNGEHEAPEATPVKILVVVGTRPEAIKLVPMILALKESDLFIPQ
                     VVSTGQHQDMVKEVFELAGIEIDFNLWVGGARSELNDRVREVMGRLDDFVRVQYGADG
                     TVKQEEVAAGDYPMAIMVQGDTTSAFAAALAAFHLRIPVIHLEAGLRTGSNLTPFPEE
                     LNRELITCIAAFNLAPTSHNLENLVRENVPVSQIFITGNTGIDSLQWAAGLEVEFSDP
                     IVGEICASDRRVVTVTAHRRENWGEGIDGISAGIRLLAIEHPDTYFVIPQHPNPAVRE
                     QWANLNDLPNICLTDALSYPEFARLMVRSFLLITDSGGIQEEAPSLGKPVLVCRDSTE
                     RGEGIEAGTLKLVGTEPASIQAGADLLLRDENAYREMSEADNPYGDGHASERIVAALT
                     NLYIGGPMPQPFRSSYSRNAVIKASGYTLPAAATGRLDPALVPEAERHHEEVVVDPVG
                     LLWSEFGTITEKWFRT"
     gene            complement(3062..4294)
                     /locus_tag="P5138_01165"
     CDS             complement(3062..4294)
                     /locus_tag="P5138_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRV59216.1"
                     /translation="MTLVTELDLPELDPADPELRGDRWHVAANGLLDSGKWVAQGPLS
                     LIIVEREAGEFFLRSKSTEFPGKLIGDLFGVTDGPLREEIDANIINTNGDVHRRLRSL
                     VNPALTPRAANGWRPVMREILEGLWDELETDEFDFVSRFSRPYPSQTIARVMGAPAVD
                     AQRLHDWSEWIQRQFDPIALSDPETVQAIQDKVAEFYAWVDPMIEDRRGNPADDLITT
                     LIRTEEEGEKLSDQELRNLVLNILVGGVDTTQSQLSHAIRLLAGKPEQWQALRENPDE
                     LVPRAVSEVLRFEPITAFTARQVTEEVEYRDVTFPAGTLMVICSFTGNRDPEAFEDAR
                     EFDILIERDRTRPLTFGAGIHYCVGANLARAELEEGLRFLAERVETFELNGLTELQAV
                     SGIYGIDRMDVRIVPDRL"
     gene            complement(4322..4948)
                     /locus_tag="P5138_01170"
     CDS             complement(4322..4948)
                     /locus_tag="P5138_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59217.1"
                     /translation="MLRCFLQGTDDAKLDTQFYLEPRPGITGFTINGGDVYTNDPRVQ
                     LSVRWPFGNYFMTASNDGGFGKSKDFDLQDTIPWTLQSSGPERLPKTVYMIFNGGGNR
                     YTDDIILDETAPVISSATVGDAGGSSILGLHSLSIRGHARKVTLRVRARDRTSGVARI
                     QVGPRKSRKLKKLKFRSRIRVNAPGRKLWVRVFDRAGNGSRWKLVRIR"
BASE COUNT         1025 a         1744 c         1701 g         1019 t
ORIGIN      
        1 gttacacccg cgggaagtgg cgcccctaca ggaccggaca gtggtcctgg agctacgtaa
       61 acatcgtcgg gctcgttccg accttctacg acatctacgg ccagggccat tactggcgga
      121 ccggcacggg cgggacgatc ttcaccccct tcaacggact gcgggccggc tactacgccg
      181 tctatcaggt ggcgtactgg gcggcttcca agaagaagcg caccaagttc acgccgatct
      241 acccggacgc gcggtcgtac tgcacattcg tctaggaatc ggccgccggg tggatctgct
      301 ctcgggaggt cttggcccag gtgtcacggc tcgtgaattg ccggatgctc gagcggaaga
      361 ggaccggcca gagcagccag gtgtagaagg cgtagatctg gccggtgagc aggcccttga
      421 tgattccgag caggatgttg cgatcctcga tctttgccgc gatggaaccg agcaccgtgc
      481 caccgaagcc gagcagatag aggaagtaga gccaccacca atcggaggcg ttggcgaaga
      541 aggaaatgcc ttcgaagaag aacaggtaga tggaagcgat cagcgaagcg ccgacgatca
      601 tctgccacac gggcatcatc aggtagacca cctgctcgaa gcgggccggg aaatagagct
      661 tcgaggtgac catgtcatcg atgagtccca tcgcctggag gttcccctgg gaccagcggg
      721 tgcgctgacg gaagagtcgc ctcagaccgg gcaggccctg ttgctcgacc gtcgcccggt
      781 tgtcgtggtg acacctccag ccggcaacca gcagcctcag acccaggtcc tggtcctcgg
      841 tcaggcggtc gcgccagggt ccacgactca cccggtcggt ttccacatcc ggttccgggt
      901 caccctcaac gatcgcctcc tctcccggcc cggggtcgcg atcgtcgata gaaatcagcg
      961 cgtccatgcg gttgtactgg ccgtttccgc ccatgccggc cgtcccccac ttgttgcgac
     1021 cggcctggaa gaggtgtccg tagatcgaga actcaaggtc ctggaaccag gtcaggacct
     1081 ggtggcggtt atagatccgg accagggtct ggacccctcc cacttcggat tccatgaagt
     1141 gctgggcgac aaagggcagg ctgtccgggg cgatgcgccc gtcggcgtcc accacgcaga
     1201 agatcgtggt ctccggattg aacttgaaac gggaagtcac ctcgtggaag gcgaagttga
     1261 gagcggcggc cttgccctgc cgggcttccg gcggtttccg ctcgaccacg tacaggtccg
     1321 gatcggtccg ctcagccagt atttccgcgg tgcggtcgtc ggatccgtcg ttgatcacga
     1381 cgatccgctt gcgcctgatc tcaagcgcct cgagccgact gacgctgtcg gcaatcgtca
     1441 cctcctcgtt cagggccggg acgaggaaca cccattcaaa gagttccgga ttcacctcgt
     1501 cacgatgcac catctcccgg gtcgacctga ccccccggaa gaagagcacg atcgtccaga
     1561 agaacattgc caccacgatc agaaaagtga tcatgaacag ggcctggaag aaaggatcca
     1621 ggccctggaa aggcaaccac tcggctgcga tcaggtgcgg aaccacttct ccgtgatcgt
     1681 cccgaactcg gaccagagca acccgacggg atcaacaact acctcttcgt gatggcgctc
     1741 ggcttcgggc acgagggccg gatcgagacg gccggtcgcc gccgccggca gggtgtatcc
     1801 ggatgccttg atcaccgcgt tgcggctgta tgaagagcgg aagggctggg gcatcggccc
     1861 gccgatgtag aggttcgtca gggcagcgac gatcctctcc gatgcgtggc cgtctccgta
     1921 gggattgtcg gcctcgctca tctcgcgata ggcgttctca tccctcagca gaagatctgc
     1981 cccggcctgg atcgaggctg gttcggtgcc gacaagcttc agggttccgg cttcgatgcc
     2041 ttccccgcgc tcggtcgaat cgcggcacac aaggaccggc ttgcccagcg aaggagcctc
     2101 ctcctgaatg ccgcctgaat cggtgatcag caggaacgag cgaaccatca ggcgggcgaa
     2161 ctcgggatag gagagcgcat cggtgaggca gatattcggc aggtcgttca ggttcgccca
     2221 ctgctcccgc accgccgggt tcggatgctg tggaatcacg aaataggtgt cgggatgctc
     2281 gatcgcgagc agcctgatgc cggccgatat cccgtcgatc ccctcgcccc agttttcccg
     2341 gcggtgggcc gtgacggtga cgacccgtcg atcgctcgcg cagatctccc ccacgatcgg
     2401 atcggagaac tcgacctcca gtccggccgc ccattgaagc gagtcgatgc cggtgttgcc
     2461 ggtgatgaag atctggctga ccggcacgtt ctcgcggacc aggttctcga ggttgtgtga
     2521 agtcggagcc aggttgaaag ccgcaatgca ggtgatcagc tcccggttga gttcctccgg
     2581 gaagggggtc aggttggacc cggtgcgcag gccggcttcc aggtggatca ccgggatcct
     2641 caggtggaag gccgccaggg ccgccgcgaa cgcagaagtg gtgtcgccct ggaccatgat
     2701 cgccatcggg tagtcacccg ccgcgacctc ctcctgtttg accgttccgt cggccccgta
     2761 ctggaccctc acgaaatcgt cgagccggcc catcacctcg cggacccggt cgttgagttc
     2821 actgcgggcc ccgccgaccc agagattgaa gtcgatttcg attccggcca actcgaacac
     2881 ttccttgacc atgtcctggt gctggcccgt ggaaacgacc tgcgggatga acaggtcgct
     2941 ctccttcagg gcgaggatca tcggcacgag ctttatcgcc tcgggcctgg ttccgaccac
     3001 caccagaatc tttaccggcg tcgcttccgg cgcctcatgc tcaccgttca tcggcagcag
     3061 actacagtcg gtcgggcacg atccgcacat ccatgcggtc gatgccataa atcccgctca
     3121 ccgcctgcaa ctcggtcaac ccgttcagtt cgaaggtttc gacccgctcg gcgaggaagc
     3181 gcagaccctc ctccagctcc gcccgggcga ggttggcccc gacgcagtag tggatgccgg
     3241 ccccgaaggt gaggggccgg gtgcggtcgc gctcgatcag gatgtcgaat tcgcgggcgt
     3301 cctcgaaggc ttcggggtcg cggttgccag tgaacgaaca gatcaccatc agggtcccgg
     3361 ccgggaacgt cacatcccgg tattcgacct cctcggtgac ctggcgggcc gtgaaggcgg
     3421 tgatcggctc gaagcgaagc acctcgctga ccgcccgggg aaccagctca tccgggttct
     3481 ctctcagggc ctgccattgc tccggcttgc cggccagaag gcggatcgcg tgggagagct
     3541 ggctctgggt ggtgtcaacc ccgccgacca ggatgttgag gacgaggttc ctgagctcct
     3601 gatcggagag cttctcccct tcctcctcgg tgcggatcag ggtcgtgatc aggtcatccg
     3661 cggggttgcc ccgccggtct tcgatcatcg ggtcgaccca ggcgtagaac tcggccacct
     3721 tgtcctggat cgcctggacc gtttccggat ccgacagcgc gatcgggtcg aactggcgct
     3781 ggatccactc ggaccagtcg tggagccgct gggcatctac cgccggggcg cccatcaccc
     3841 gggcgatcgt ctgcgacgga tagggccggc tgaagcggga gacgaagtcg aattcatcgg
     3901 tctcgagctc gtcccagagg ccttcgagga tctcgcgcat caccggccgc cagccgttcg
     3961 cggcacgggg ggtcagggcc gggttgacga ggctcctgag ccggcggtgg acgtcgccgt
     4021 tggtgttgat gatgttggcg tcgatctctt cccgcagggg gccgtcggtg acgccgaaaa
     4081 gatcgccgat cagcttgccg ggaaactcgg tcgacttgga gcgcaagaag aactcgcccg
     4141 cctcccgttc gacgatgatc agagacagcg gcccctgggc cacccacttt ccggagtcga
     4201 gcaggccatt tgccgccaca tgccagcgat cccccctcag ctcgggatca gccggatcca
     4261 gctcgggcag gtcgagttcg gtaaccaggg tcatgccggg agcctactcc cgtacaaacg
     4321 gctacctgat gcgaaccaac ttccagcggg agccgttacc cgcccggtca aagacccgaa
     4381 cccagagctt tcgccccggt gcatttaccc gaattcggga cctgaacttc agcttcttga
     4441 gcttccgcga cttacgcgga cctacctgga tccttgccac gccagaagtc cggtccctcg
     4501 cccgcacgcg cagagtcacc ttcctggcat gaccgcgaat cgatagtgaa tgaagaccca
     4561 agatcgagct ccccccggca tcgccaacgg tcgctgaact gataaccggc gcagtctcgt
     4621 caagaatgat gtcatcggtg tagcggtttc cgccaccgtt gaagatcatg tacacggttt
     4681 tcggcagcct ttccgggcca ctcgactgga gggtccatgg aatcgtgtct tgaaggtcaa
     4741 aatcctttga cttgccgaaa ccgccgtcat tcgacgctgt catgaagtag ttgccaaagg
     4801 gccacctgac agaaagctga actcgggggt cgtttgtgta aacatcgccg ccgttgatgg
     4861 tgaaaccggt aattcccggc ctcggctcca gatagaactg cgtatccagt ttcgcgtcgt
     4921 cggtgccttg caggaagcaa cgaagcacgt ctccgtttcc gtctgggccg atacagtttc
     4981 cattcctgaa gtttccccca cccgtttcgt acttctcccg gaaccagaag ccttgccaac
     5041 tggtccggtt cccgggatcc acggtcagta cgactggtat accggccccg aaattcgcta
     5101 cgcaggtccc aggacagtca atcccggacg gtgccgaggt gattcttacg ggacccgaat
     5161 tctcgccgtg aaggttgacc gaaacctggt gtgccgaaga cccggcggcc aactgcccga
     5221 gtgcgacgca agaaagaagt aaaccggcga aaaataatgc gagcttcgta agtggacgcc
     5281 tcatgcgaat ctccctttga gttgaacgaa cgcggcaccc taacagggcc gaccggcccg
     5341 gtcaatccgc gaacttgtag ccgatgcctc tgaccgtgag gaccagctcg gggcgctggg
     5401 ggtccgtttc gaccttctga cggaggttcg agatgtggac gtcgcaggca cgttcatcgc
     5461 cgtcgaactc cgtctgccag agctcttcc
//
LOCUS       DAOTIP010000033         5445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17789_length_5445_cov_3.320779, whole genome shotgun sequence.
ACCESSION   DAOTIP010000033 DAOTIP010000000
VERSION     DAOTIP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17789_length_5445_cov_3.320779"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="P5138_01175"
     CDS             complement(<1..330)
                     /locus_tag="P5138_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRV59218.1"
                     /translation="MADLQLLAASGSETAARIASGNVSAGEVLDFWLERAAGDPLNGY
                     LWHADAEAARTGVSADGGALSGVPLAIKDIFCTEGVPTTAASKILGGYVPPHTATSVA
                     RTAAAMPS"
     gene            complement(334..624)
                     /gene="gatC"
                     /locus_tag="P5138_01180"
     CDS             complement(334..624)
                     /gene="gatC"
                     /locus_tag="P5138_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRV59219.1"
                     /translation="MIEREQVLHVARLSRLRLGDEELDTLAGELSSILDHVDKLAEVD
                     IEGVEPTSHVVPLENVLRPDEPRPSLERDVALSQAPDPEDGAFRVPSPQAEA"
     gene            complement(671..1096)
                     /locus_tag="P5138_01185"
     CDS             complement(671..1096)
                     /locus_tag="P5138_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59220.1"
                     /translation="MVALGWNGMNGDQAASGFSFSRVHPLEWLAGLCGIALICALIMP
                     WSGGKASSDSPGLLGVLLLLNGVFAFLLPFVVASSPRTNVPIVYETFLWTVTLLLTIV
                     LLVKLAFPPDGGFDSGFWLALGATLVASLSLWRSVGRER"
     gene            1150..2190
                     /locus_tag="P5138_01190"
     CDS             1150..2190
                     /locus_tag="P5138_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRV59221.1"
                     /translation="MGLIRGACLTNFRELMFELGADPDPVLRGAGIRPQDAGNHEVFL
                     SYRSLILTLERAAEVTGRPDFGRRLALLQGIEILGPVGVAARTAPTTGDALQACSTYL
                     SAYSPAIAVRLVPQDNGDRVFLEFEIVDHSVPPAMQVIELSLGVALRVVRYLRGEGYR
                     PAAVHLPHAPLARQSDYSAYYGCEPCFNSPAAGLMLRESDLAKSVSQDAQAHEAIARY
                     LDTIVDSDVQGMSGPVRQLVRQLLPTGAVGLEQIAGQFALHPKTLQRRLNVEGTSFTF
                     LVEQCRQELAEHYLRDTDLSLIQVARELGYTEQSVLTRSCQRWYGRSPSALRRDLRDP
                     ERALGRLKTATV"
     gene            2383..2943
                     /locus_tag="P5138_01195"
     CDS             2383..2943
                     /locus_tag="P5138_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HRV59222.1"
                     /translation="MTAATTPAPATTANGNPLPLLALPQGELLTINEGDTPLIKDALG
                     PGVHFKPLRLDLEQGEWVVQATFAPGARIPLHYHTGAVDAWTLSGSWHYEEYPDQPQT
                     AGSYLYEPGASVHTLYVPEDNTEDTVVHFRVSGANVNFDEEGSFHSVLDACMIAHLTT
                     ELCEAQGIGPVDYVRGGFAAVKSDAS"
     gene            2943..3974
                     /locus_tag="P5138_01200"
     CDS             2943..3974
                     /locus_tag="P5138_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59223.1"
                     /translation="METKTEEGRPAPSLDMRVFITGANGFIARAVATRLRELGATVSG
                     MDLEADPSRGVIAGDITEPGDWTEELAGADVVIHTAALLGPAYSLERSRRVNVYGTHQ
                     VLRAAISAGVGRFVHFSSIMAYGFGFPDGVDETYPVHVDNDVYTDTKVNSEAVVLAAH
                     AAGEIDVTVVRPGDVWGPGSIWVRVPLAEMAKPTGFPIPDGGNGIFTPVYIDNFVDGL
                     ILALANPEQTRGQIFNISDGIGIPCSEYFGRLSRMGGGRVVTMPVRVAAPLASAVGGL
                     IRMFGTHSDLAAGTMYMLNRKGTVSIEKARTVLGYEPLVSFDEGMDRVEEWAMSEGIL
                     PDSNLAEVA"
     gene            3974..5068
                     /locus_tag="P5138_01205"
     CDS             3974..5068
                     /locus_tag="P5138_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRV59224.1"
                     /translation="MRAVLMKDGQLAVEEIAEPTPGPGEVLVETLAVGICGSDMSAFA
                     NGEGFNRAALALTGQEMLDLDNPIVLGHEFVARILSYGPETGQQHPVGTRVVAMPMLL
                     RDPQVLIGFAGVDTPGAYAERFILSEDLLIPVPDEMSTELAALTEPMAVALHAVNRVD
                     VDSNYVPLVLGCGPIGLAIVAMLKMRGLGPIVAVDLSPERLELAKKLGADEVIDPTVN
                     SPYEAWMQAAATDDPALMAAPTAVIGQLPLRPTVGFESTGKPGLLKQMIDGAPPGSRI
                     SVAGINLGTDEFEPSHGILKELDLRFCLYYTPEEFAQTVSLLDSGRLDVSAMVTGRVG
                     FEGVAEAFERLAQSPADGKILIDPGTTVPA"
     gene            complement(5074..>5445)
                     /locus_tag="P5138_01210"
     CDS             complement(5074..>5445)
                     /locus_tag="P5138_01210"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935057.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRV59225.1"
                     /translation="GAEAVIVGRSNLVGRPLISLLLGRNATVTAAHSRTRDLPGVCSR
                     ADVLVAAVGVPELLGADHVKPGATVIDVGINRTDDGLKGDVDFDAAVEIAGAITPVPG
                     GVGPMTIAMLMANTLTAARAR"
BASE COUNT          964 a         1743 c         1772 g          966 t
ORIGIN      
        1 gctgggcatc gccgccgcgg tcctggccac cgaggtggcg gtatggggcg gcacatatcc
       61 gccgaggatc ttcgaggcgg cggtggtcgg cacgccctcg gtgcagaaga tgtccttgat
      121 cgccaggggc actccgctca gggcgccgcc atcggcactg acccctgtcc ttgccgcttc
      181 ggcgtcggca tgccagagat agccgttgag ggggtccccg gccgcccgct caagccagaa
      241 atcaagcacc tctcctgccg atacattgcc ggacgcgatc cgggccgctg tttcgcttcc
      301 gctggcggcc agcaactgga ggtcggccat cgcctatgcc tctgcctgcg gtgatggcac
      361 cctgaaggcg ccgtcttccg gatcgggtgc ctgggagagc gcgacgtccc gttcgaggct
      421 gggcctcggc tcgtccgggc gcaggacgtt ctcgagtggc accacatgcg aggtcggctc
      481 gacaccttcg atgtctacct cggccagctt gtctacgtgg tcaaggatcg aggagagttc
      541 cccggccagg gtgtccaact cttcgtcgcc cagccgcagt ctggagaggc gggcgacatg
      601 gagaacctgt tcgcgttcaa tcacgaagct gagtctatgg acgagccagc ggcggcgtca
      661 ggcagccctg tcagcgttcc cgaccgacgc ttcgccaaag cgagaggctg gcaaccagag
      721 tggcacccag ggccagccag aaacccgaat cgaagccgcc gtccggcggg aaagccagct
      781 tgaccaagag cacgatcgtc aacagcaggg taaccgtcca gaggaaggtc tcgtagacga
      841 tcggcacgtt ggtccggggg ctgcttgcga caacgaacgg aagcaggaat gcgaaaacgc
      901 cgttcagcag gagcaacaca ccgagcaggc ccggcgaatc ggaggatgcc ttgcccccgc
      961 tccagggcat gatcagggcg cagatcagcg cgattccaca aaggccggcc agccactcga
     1021 gcgggtgaac ccgcgagaag ctgaaacccg aggctgcctg atcaccattc attccgttcc
     1081 accctaacgc gaccatggcc tcgaccgtgc ccttgacaga gcgaaaggac tctgagaccg
     1141 tctcgaatcg tgggcttgat acgcggggca tgtctgacca acttcaggga gttgatgttc
     1201 gagttgggtg ccgacccgga tcctgtgctt cgcggtgccg gaatacgccc gcaggatgcc
     1261 ggcaaccatg aggtctttct cagctaccgg agtctgatcc tgactctcga gcgggccgcc
     1321 gaagtgaccg ggcggccgga ttttggccgt cgccttgccc tgcttcaggg aatcgagatc
     1381 ctcggcccgg tcggcgtggc agcacgaacg gcgccgacga ccggggatgc gctccaggcc
     1441 tgcagcacgt atctgtcggc ttacagtccc gcgatagccg tccggttggt cccccaggac
     1501 aacggggacc gcgtctttct cgagttcgag attgttgatc attcggtccc cccggcaatg
     1561 caggtgatcg aactttcgct cggggtcgca ctccgtgtcg tccgctatct ccgcggtgag
     1621 gggtaccggc cggcggctgt gcatctcccg catgcaccgc tggcgagaca aagcgactac
     1681 agcgcctact acggctgcga accctgcttc aactccccgg ctgccggact gatgcttcgc
     1741 gagtccgacc tggcaaagtc cgtgtcgcag gatgcccagg cgcacgaagc gatcgcccgg
     1801 tacctggaca ccatcgtcga ttcggacgtg cagggaatga gcggtccggt tcgccagctg
     1861 gttcgccagt tgctgcccac cggggccgtt ggcctggaac agatcgcggg acagttcgcg
     1921 ctccacccga agacgcttca gcgacggctg aatgtcgagg gaacgagctt cactttcctg
     1981 gtcgagcagt gccgccagga actcgccgag cattaccttc gagacacaga cctcagtctg
     2041 atccaggttg cgcgcgaact tggctacacc gaacagagcg tcctcacccg ttcgtgccag
     2101 cgttggtacg gccgcagtcc ttcggctctg cgccgcgact tgcgcgaccc tgaacgagcg
     2161 ctcggccggc tgaaaaccgc aacggtctga cccctccggg tcactcctgg agctatgtcc
     2221 tcaactatca aaaacttgtc ctgaaatgtc aagacatgac aactggccgg tgttacgatc
     2281 gacggaatcg ccgcccgcgc ggtgaggatt ctggcatttg ccgggagaga gagaaagtca
     2341 agtgctcagg cgcctgttcg ggccttcaga aaggactggt cgatgaccgc tgccaccacc
     2401 cctgctccgg cgacaaccgc gaacggcaat cccctgccgc tgctcgcgtt gccccagggc
     2461 gaactgctga ccatcaacga aggcgacacg cccctgatca aggatgcgct cggtcccggc
     2521 gttcacttca agccgttgag gctggacctc gagcagggcg aatgggttgt acaggcgact
     2581 ttcgcgccgg gtgcccgcat cccgctccac taccacaccg gtgcggtcga tgcctggact
     2641 ttgtccggat cctggcacta cgaggagtac ccggaccagc cccagacggc gggctcgtac
     2701 ctgtacgagc cgggtgcttc ggttcacact ctgtacgttc ccgaagacaa caccgaggat
     2761 acggtcgtcc acttcagagt ctccggagcg aacgtcaact ttgacgagga aggctccttc
     2821 cactcggttc tcgacgcctg catgatcgcc cacctgacga cggagctgtg cgaggcccag
     2881 ggcattggtc cggtcgatta cgtccgcggc gggttcgccg ccgtcaagag cgacgcgagc
     2941 tgatggagac taagacggaa gaggggcgcc cggctccgtc cctcgacatg cgtgtgttca
     3001 tcacgggcgc gaacggcttc atagcgcgcg ctgtggcaac gcgcctccgc gagctcggcg
     3061 cgacggtcag cggaatggat ctcgaagcgg atccttcgcg cggggtaatt gcgggcgaca
     3121 tcacggagcc gggagactgg acggaggaac ttgccggcgc cgatgtcgtc atccataccg
     3181 cggctttgct cggtccggcg tacagtctcg agcggtcccg gagggtcaat gtctacggga
     3241 cccaccaggt cctgcgggca gccatctctg ccggcgtcgg tcgtttcgtg cacttctcgt
     3301 caatcatggc ctacggcttc ggctttccgg acggtgtcga cgagacgtat ccggtgcatg
     3361 tcgacaacga cgtgtacacc gacaccaagg tgaactcgga agctgtcgtg ctcgcggctc
     3421 atgccgcggg cgagatcgat gtcaccgtgg tgcgccccgg agatgtctgg ggtcccggtt
     3481 cgatctgggt ccgcgtacca ctcgccgaaa tggcaaaacc gaccggcttt ccgattcctg
     3541 acggcgggaa cgggatcttc accccggtat acatcgacaa cttcgtcgat ggcctgatcc
     3601 tggccctggc caacccggag cagacccggg ggcagatctt caacatctcc gacgggatcg
     3661 gaattccctg ctccgaatac ttcgggcgat tgtcccggat gggcggcggc cgcgtggtga
     3721 ccatgcccgt acgtgtcgcg gctccgctcg cgagtgcggt tgggggattg atccgaatgt
     3781 tcggtacaca cagcgacctg gccgcaggaa ccatgtacat gctcaatcgc aagggcacgg
     3841 tctcgatcga gaaggcccgc acggtgctcg gctacgagcc gctcgtctca ttcgacgagg
     3901 ggatggacag ggtcgaggaa tgggcaatga gcgaaggaat cctgccggat tccaacctgg
     3961 cggaggtggc ctgatgcgcg cggttctgat gaaggacggt cagcttgccg tcgaagagat
     4021 tgcggagcca acgccgggac ccggcgaggt gctggtcgaa acgcttgccg tgggcatctg
     4081 cggttccgac atgagtgcgt tcgccaacgg cgagggtttc aaccgcgccg cccttgccct
     4141 gaccggccag gagatgcttg atctggacaa cccgattgtt ctaggccacg aattcgttgc
     4201 ccggatcctg tcctacggcc ccgagacggg acaacagcat ccggtcggca cccgggtcgt
     4261 ggcgatgccg atgttgctgc gggatcccca ggtgctgatc gggtttgccg gcgttgacac
     4321 cccgggcgcg tatgcggaac gtttcatcct ctccgaggac ttgttgatcc cggtgccgga
     4381 cgaaatgtcc actgaactcg cggcgctgac ggagccgatg gctgtggcgc tccacgcggt
     4441 gaaccgggtg gacgtcgatt cgaattacgt accactcgtc ctgggctgcg ggccgatcgg
     4501 actggccatc gtcgcgatgc tgaaaatgcg cgggctggga ccgatcgtgg cggtggacct
     4561 gtccccggag cgactcgagc tcgcgaaaaa gctgggggcg gacgaggtga tcgacccgac
     4621 cgtgaactcc ccgtatgaag cctggatgca ggccgcagcg acggacgacc cggcactgat
     4681 ggccgcgccg actgccgtga tcgggcagtt gcccctgcgc ccgacggtcg gcttcgaatc
     4741 gacgggcaag cccggtctgt tgaagcagat gatcgacggg gcgcccccgg gctcgcgcat
     4801 atcggtagcc ggaatcaacc ttggcaccga cgaattcgag ccttcccacg ggatcctgaa
     4861 ggagctggac ctgcgcttct gcctgtacta cacgcccgag gagtttgcgc agaccgtgtc
     4921 gcttctggat tcaggtcggc ttgacgtctc ggcgatggtg acaggcagag tcggcttcga
     4981 gggagtcgcc gaagctttcg agcggctggc tcaatcaccg gccgacggga agatcctcat
     5041 cgaccccgga accacggtcc cagcctgatg tgattagcgg gcccgggcgg cggtcagcgt
     5101 gttggccatc agcatggcga tggtcatcgg gccgacgccg cccggcaccg gggttatcgc
     5161 tcctgctatc tccaccgctg catcgaagtc gacgtcaccc ttcaggccgt catcggtccg
     5221 gttgatgccg acgtcgatca ctgtggcgcc cggtttcaca tggtcggcgc ccagcaactc
     5281 gggaacgccg acggcggcga cgagcacgtc ggcccgcgag cagacgccgg gcagatcacg
     5341 ggtccgcgag tgagctgcgg tgaccgtggc gtttctcccc aggagcaggc tgatcagcgg
     5401 tcgcccgacc aggttggagc ggccgacgat tacagcctcc gcccc
//
LOCUS       DAOTIP010000034         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_17974_length_5405_cov_3.325421, whole genome shotgun sequence.
ACCESSION   DAOTIP010000034 DAOTIP010000000
VERSION     DAOTIP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17974_length_5405_cov_3.325421"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1535)
                     /locus_tag="P5138_01215"
     CDS             complement(162..1535)
                     /locus_tag="P5138_01215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV59226.1"
                     /translation="MSIHEFDVVVLGAGPAGEVFAGRSADAGLKVGLVEQHLVAGECS
                     YYACMPSKALLRPGELLDEARRTEGVRQAVTGILDADSVLARRDEVIHHLDDSSQMGW
                     LERKGIELFRGTGVLAGEREVRVAGEVLVANRAVAIATGSSAAVPFIPGIGEVSIWNN
                     HQATTAETVPESLIILGGGPVGCELAQAWASLGTEVTLLEAEDRVLPGEEPFAGEQVE
                     QALREEHGVNLMTGAAVDSIESTDGQITATAVGRQYTASHLLVATGRRPRTDRIGLES
                     IGIDGGGHLETDDCYRVKGHDWLYAVGDVNGRTLLTHMGKYQSWIAVQTLLGHEPDNR
                     AESIGAPRITFTNPQVAAVGKTLAAAEADGMDVVVIDASTGSSAGASFVGKGVGGTSR
                     LVIERSTGLIVGATFTGFEVAEWLHAATIAIVGKVPMDELRHAVAAFPARSEIWLRFS
                     EKWEARQ"
     gene            1636..1997
                     /gene="ssrA"
                     /locus_tag="P5138_01220"
     tmRNA           1636..1997
                     /gene="ssrA"
                     /locus_tag="P5138_01220"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2206..3033)
                     /locus_tag="P5138_01225"
     CDS             complement(2206..3033)
                     /locus_tag="P5138_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59227.1"
                     /translation="MKLRLVTFLVLIAAGLALTASPASAQRPIPGIKQTVTFKQMKSY
                     VSFLFTKRNSPTPNVRRKKYKTTLTARRKNASLTVDKLFARTLLRLSKQDDNQQRRIV
                     KQILTNQKRQVQAIKQDLAERLAGLQDDQNAAIQRIYNKYAPQINFKANKRDRLKRQL
                     SRTTNPARRAKLIRQINKLQTQINGLVSDRNTDVNSVNSRYKARITSVTNLYNSRMAN
                     VKANAQQQIQQAKNAWRRTFRTQLVAAKARRGAQRDLVNTVAARGFGYIQQMPPAGE"
     gene            complement(3112..5091)
                     /locus_tag="P5138_01230"
     CDS             complement(3112..5091)
                     /locus_tag="P5138_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRV59228.1"
                     /translation="MSNGGFDPHPHSGPQFGQTWEARIQTPESVPGVSPYPPIADYGF
                     LSDGEVSALVAPSGNIEWLCLPRFDSPSVFGSILDRSAGNFRLGPADINVPSHRRYLP
                     GTNVLETSWGTGDGWIIVRDVLLIGPWHHEGDRSGTHRRAPTDYDADHVLLRMVRCVH
                     GEVQLTLDCKPVFDYGRHPAKWEYTGDAYHEGVASAEGTDIQLRLNTDMNLGFEGPRT
                     TARTLLKEGDVRFVALSWSEHAAPQDYDEAYDRLVWTAHHWQNWLARGTFPDHPWRGY
                     LERSALTLKGLTYAPTGALVAAATTSLPETPGGERNWDYRFSWIRDSTFTLWGLYTLG
                     FDWEANDFFYFIADVAAGEDDLQIMYGVAGEKQLDEEILDHLHGYEGASPVRIGNGAY
                     DQRQHDVWGAVLDSFYLHTKSRDGMPEEIWPILKRQVTAALDHWRETDHGIWEVRGKP
                     QHFTSSKVMCWVAVDRGARLARLREEHDLANEWQVAADEIHADICENAVDERGVFTQH
                     YETDALDASCLLLPLLRFLPATDPRVRRTVLAIADELTEDGLVLRYRVDETDDGLSGE
                     EGSFTICSFWLVSALVEIGELERARDLCERLLGYASSLQLYAEEIDPRSGRHLGNFPQ
                     AFTHLALINAVMHVIRAEQETTAPRDKTTSPRANP"
     gene            5155..>5405
                     /locus_tag="P5138_01235"
     CDS             5155..>5405
                     /locus_tag="P5138_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV59229.1"
                     /translation="MAVVTATSIGIHVGGDPLFSEVSFKLMRGDRMTLSGRNGAGKST
                     LLRILAGEAENDSGALSYQKGARVALHDQRPPRDRGLSL"
BASE COUNT          888 a         1691 c         1808 g         1018 t
ORIGIN      
        1 ccggcaaggg ctccgatcgt ttcgtccagg ccctggtgaa tcgaagcgaa gtcacccgcc
       61 ccgatcacca cgtcggcctc cttcgacatc tcggtcaggc gggccgcctg gtccaggtca
      121 cgatgcaagt cactgaaagc gaggatcttc atgggggaag cctactggcg ggcttcccac
      181 ttctcgctga aacggagcca gatctcactc cttgcgggga aggcggccac cgcatggcga
      241 agttcgtcca tcggtacctt gcccacgatc gcgatcgtcg ctgcatggag ccattcggcg
      301 acctcgaagc cggtgaaggt agcaccaacg atcaggccag tcgaacgttc gatcaccagt
      361 cggctggtcc cgccaacgcc tttcccgacg aaggaggcac ccgccgagga gccggtgctc
      421 gcgtcaatga cgaccacatc catgccgtcc gcttcggctg cggcgagggt cttcccgacc
      481 gcggcaacct ggggattggt gaaggtgatt cggggcgcgc cgatcgactc tgcccggttg
      541 tcgggttcgt gacccagaag cgtctgaacc gcgatccatg actggtactt gcccatgtga
      601 gtgagcaggg tccggccatt cacgtcgccg accgcataca gccagtcgtg ccccttcacc
      661 cggtagcagt cgtccgtctc gagatggcct ccgccatcga ttccgatcga ctcgagaccg
      721 atccggtcgg tccggggtcg ccggccggtc gcaacgagaa gatgcgacgc cgtgtattgc
      781 cggcccaccg ccgtggccgt gatctgcccg tctgtcgatt cgatcgaatc gaccgcggcc
      841 ccggtcatca ggttgacccc gtgctcctcc cgcagggcct gctcgacctg ctcgccggcg
      901 aacggctcct cgccggggag aacccggtct tccgcttcga gaagggtcac ttccgtgccg
      961 agactcgccc aagcctgggc cagttcgcag ccgaccgggc caccgccgag aatgatcagg
     1021 gattcgggaa cggtctccgc tgtggtggcc tgatggttgt tccagatcga cacttccccg
     1081 attcccggaa tgaacgggac tgcggccgaa gacccggtgg cgatggcgac cgcccggttg
     1141 gcgaccagca cttctcccgc aacccggacc tctctttcgc cggccagcac cccggtgccc
     1201 cggaacagct cgattccctt tcgctccagc cagcccatct ggctggagtc gtcgaggtgg
     1261 tggattacct cgtcgcgacg ggcgaggacg gagtcggcgt ccagaatccc ggtgacggcc
     1321 tggcgaaccc cctccgtcct ccgcgcctcg tcgagcaact cccccgggcg aagcagcgcc
     1381 ttcgacggca tgcaggcgta gtaggaacat tcaccggcga ccaggtgctg ctcgaccagg
     1441 ccgaccttca ggccggcatc tgccgatcgc ccggcgaaga cttctccggc cggtccggcg
     1501 ccaagaacta cgacgtcgaa ttcgtggatg ctcaagattt ctttcgcatt ggttacgcct
     1561 tcccgacccg gtgcggggct gtcagctctt tgacccggac ttcggcacac cgtacaatgg
     1621 aggggcacca tccaagggga cgacaggctt cgacgttgtc ggaccgtgcg gagggttgca
     1681 ggtcgaggac gccgatggcc tcgtaaaaaa tcggcacaaa ccaatacgtg cggaccacga
     1741 gttcgccctc gccgcctagc tgatacctag gtaacgagag tcggcccacc ctcggcccgt
     1801 ggggaggggt tccggcttca gaaacgggct tgccccgaag gatcgcctcg gccggagggg
     1861 gacatttcag aggcttggcc attcgtagtg cctgctggcg cggaccccgg atggcgagaa
     1921 caaagcgccg gctaagcctg tagacgcctt tcgtcacgcg actgcggacg cgggttcaat
     1981 tcccgccgtc tccatcagag ctccgaagag aaatcccggg gccgtgaaga aagagaaagg
     2041 gcccgcctgg ttagcgggcc cttcttgctt cccggacggt gcccggttcc tctcggaccg
     2101 tgctcggttc gatttacccc cgtatatcag gggcaaattg aaccgagtgg cggaaacttg
     2161 cggccggcta cggaaattcg tggtcggccg ggaaacgcgc ggtggctact cgccggccgg
     2221 gggcatctgc tggatgtagc cgaagccgcg tgccgcgacc gtgttgacca ggtcccgctg
     2281 tgctccccga cgggcctttg ccgcgaccag ctgggtacgg aaggtccggc gccaggcgtt
     2341 ctttgcctgc tggatctgct gctgggcgtt ggccttcacg ttggccatcc gcgagttgta
     2401 gaggttggtg acgctggtga tccgggcctt gtaacgggaa ttgacgctgt tgacgtcggt
     2461 attgcggtca ctcaccagtc cgttgatctg ggtctgcagc ttgttgatct gcctgatcag
     2521 cttggcccgc ctggccggat tcgtggttcg gctcaactgt cgcttgaggc ggtcacgctt
     2581 gttcgccttg aaattgatct gcggcgcata cttgttgtag atccgctgga tggcggcgtt
     2641 ctggtcatcc tgcaggccgg ccagacgctc agcgaggtcc tgcttgatcg cctggacctg
     2701 ccttttctgg ttggtgagga tctgcttgac gatccggcgc tgctggttgt catcctgctt
     2761 ggagagccgc agcagcgtgc gggcgaagag cttgtcgacc gtgaggctgg cgttcttgcg
     2821 gcgggcggtc aaagtcgtct tgtacttctt gcgccggaca ttcggcgtcg ggctgttgcg
     2881 cttcgtgaaa aggaagctga cgtagctctt catctgcttg aaggtgaccg tctgctttat
     2941 gcccgggatc ggacgctggg ccgaagcggg ggaagcggtc agggcgagac cggcggctat
     3001 caggacgagg aaggtgacga gacgtagctt catggtgtcg aacctaacac cgcgcttagg
     3061 tcgaggttgt gatttcttcg tcgaggtccg gagatggcgg gacgattccg gctacggatt
     3121 cgccctgggc gaggtggtct tgtcgcgtgg agcggtcgtc tcctgctcgg cccggatcac
     3181 atgcatgacc gcgttgatca gggccaggtg ggtgaaggcc tgcgggaagt tgccgaggtg
     3241 gcggcccgag cgcggatcga tttcctcggc gtagagctga agtgaggacg catacccgag
     3301 gagtcgttcg cagagatccc gggcgcgctc cagttcaccg atttccacca gggcgctgac
     3361 cagccagaac gagcagatgg tgaagcttcc ctcctcgccg ctcagtccgt catcggtctc
     3421 gtcgacgcga tagcggagca ccaggccatc ctcggtgagc tcgtccgcga tcgccagcac
     3481 cgtcctgcgg acccgggggt cagttgccgg gaggaagcgg agcaacggca gcagcagaca
     3541 tgaggcatcg agcgcgtcgg tctcgtaatg ctgggtgaag accccccgtt cgtcgaccgc
     3601 gttctcacag atgtcggcgt gaatctcgtc tgcggcgacc tgccactcgt ttgcgaggtc
     3661 atgctcctcg cgcagacggg cgaggcgggc gccccggtcg accgccaccc agcacatcac
     3721 cttcgatgac gtgaagtgct ggggtttgcc gcgcacctcc cagattccgt ggtccgtctc
     3781 tcgccagtga tcaagcgccg cggtgacctg ccgtttgagg atcggccaga tctcctccgg
     3841 catcccgtcc cgggacttgg tgtggaggta gaacgaatcg agtaccgcgc cccagacgtc
     3901 gtgctggcgc tggtcgtagg cgccatttcc gatgcggacc ggggatgccc cctcgtaacc
     3961 gtggagatgg tcgaggatct cctcgtcgag ttgtttctcg ccggcgaccc cgtacatgat
     4021 ctggagatcg tcctcgccgg ctgcgacgtc ggcgatgaaa tagaagaaat cgttcgcctc
     4081 ccagtcgaag ccgagcgtgt agaggcccca aagcgtgaaa gtggagtcgc ggatccagga
     4141 gaagcggtaa tcccagttgc gctcgccgcc cggggtctcc ggaagcgagg tggtcgcggc
     4201 ggcgaccagg gcgccggttg gcgcataggt caggcccttc agggtcaggg ccgatcgctc
     4261 cagatacccc cgccaggggt ggtcggggaa ggttccgcgg gccagccagt tctgccagtg
     4321 gtgagccgtc cagaccagcc ggtcgtaggc ttcgtcgtaa tcctggggag cggcgtgctc
     4381 gctccaggag agggcgacga agcggacatc accctccttg agcagggtcc gggcagtcgt
     4441 cctcgggccc tcgaagccga ggttcatgtc tgtgttgagc cgaagctgga tgtcagttcc
     4501 ctctgcggag gccacgcctt cgtggtacgc gtcgccggtg tactcccatt tcgcagggtg
     4561 gcggccgtag tcgaagaccg gcttgcagtc gagggtgagc tgcacctcac cgtgaacgca
     4621 gcggaccatc cgcagaagga cgtggtcggc gtcgtagtcg gtaggggccc gccggtgggt
     4681 tccggaacga tccccttcgt gatgccaggg gccgatcagc agcacgtcac ggacgatgat
     4741 ccagccgtcc ccggtgcccc aggaggtctc gagcacgttc gtccccggca ggtagcgacg
     4801 gtggctgggc acgttgatgt cggccggacc cagccggaag ttcccggcgc ttcggtcgag
     4861 gatcgagccg aagacactgg gcgagtcgaa ccggggcagg cagagccatt cgatgtttcc
     4921 gctgggggca accagggcgg acacctcgcc gtccgagagg aagccgtagt cggcgatcgg
     4981 cgggtagggc gagaccccgg gcaccgactc gggagtctgg atgcgtgctt cccaggtctg
     5041 tccgaactgg ggtccggagt gggggtgagg gtcgaatccg ccgttgctca tgaggcgaaa
     5101 atgctgtcac atttcctgag ccgctcccaa gctggcagac tggctggtcg ctccatggca
     5161 gtcgttacgg caacaagtat cggaattcac gtcggcggcg atccgctgtt cagtgaggtt
     5221 tccttcaagc tcatgcgggg tgaccggatg accctttccg gacgcaacgg cgccggcaag
     5281 tcgaccctgc tccggatcct cgcgggcgag gccgagaatg attccggtgc gctcagctac
     5341 cagaagggag cccgggtggc gctgcatgac cagcgtccgc cacgggaccg gggtctctcc
     5401 ctcca
//
LOCUS       DAOTIP010000035         5396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_18017_length_5396_cov_3.462460, whole genome shotgun sequence.
ACCESSION   DAOTIP010000035 DAOTIP010000000
VERSION     DAOTIP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18017_length_5396_cov_3.462460"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="P5138_01240"
     CDS             complement(<1..981)
                     /locus_tag="P5138_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRV59230.1"
                     /translation="MSPLPKIALAQVNPTVGDLDENAALVSEWIDLAAQRGAEIVVFP
                     ELVLTGYPAEDLYLRPDFIAATGERLREVAAGVGDITALVGFPEPVGNPVGRAIAANA
                     TAVISQGEIRGVYRKRLLPNYGVFDEYRTFRNGTEPMVTEAGGTRLGVTICEDCWDPG
                     SPVFTDLAPGAELIINSSASPYHRGKGLEREEIFRRVATAKGIPVAMVNMVGGQDELI
                     FDGSSALIGPDGSVLARAGQFTEDLLVYDPEEASAPVAEWMDDLDEVYTALVTGLRDY
                     VVKNGFGHVGLGLSGGIDSALVAALAADALGPEQVTCVVMPSPHSSGETQA"
     regulatory      1113..1281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1287..2237
                     /locus_tag="P5138_01245"
     CDS             1287..2237
                     /locus_tag="P5138_01245"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRV59231.1"
                     /translation="MSSSRIIRALMSLLACFAVFAALGSSATATTTAGASSEANAVST
                     AKSEGNARKGPVVLNVADYSVNYGGRFRLRGKSGRSVKGNIRIQSNNGHGWQTLRKVG
                     TDGEGRYAVKVVARVNTALRAVAGDGHRSKIRRVAVIGQVRLDRPKRYVKLGQSLRVS
                     GVVLPRGSRPIRLRVRGHGVVATRSRANGRFTLRFKPRSHGDFGYRVWALKNGLSTGD
                     SSLARRFSALRPGHASYYGPGLYGNGVACGGTLTPTTRGVANKYLPCGTKVTLQYGGR
                     TVVARVIDRGPYVAGRDWDLTEQTKKDLGFGGVGVVWTNK"
     gene            2343..2681
                     /locus_tag="P5138_01250"
     CDS             2343..2681
                     /locus_tag="P5138_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59232.1"
                     /translation="MSSAESKNLEAISKAIDHHNATCPFPAAEVRMNPFEVERLGWEE
                     IRGLPIVPDPNIGTGRFHIVCSRDMEGDELEEIEAIGEDLVTVTPGTREEPGAPEADP
                     APAENPGRWN"
     gene            complement(2682..3476)
                     /locus_tag="P5138_01255"
     CDS             complement(2682..3476)
                     /locus_tag="P5138_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRV59233.1"
                     /translation="MPFAETFHAGLAALPFAASDPAAGVGLLTALVAGIVSFLSPCVL
                     PIVPGYLSAVSGVQVSDLDRADWRKVLAPALIFCASFSTVFILLGMGATKIGSLLSDH
                     RDLLDKIAAALIIAMGILFVASFFVTRLNREWHVDALMQRAGKGGPLVAGAAFAVAWT
                     PCIGPTLGAILSAASLTDSAARGALLLAVYSAGLAIPFLLTALAFNRMTTAFAVIKRH
                     YGLIIAIGGVILIIMGILIWTGELYRINIEIQNWMTDLGIDFWNAV"
     gene            3502..4359
                     /locus_tag="P5138_01260"
     CDS             3502..4359
                     /locus_tag="P5138_01260"
                     /inference="COORDINATES: protein motif:HMM:NF005878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59234.1"
                     /translation="MEHVLEGAVVVVTGGAAGIGRATSTKMAEAGARVAIGDIHGEKA
                     AEAAEEVSKRADGAGSGGGAIGKPLDVTDREGFELFLDDVEAELGPIDCLVNNAGVML
                     LGPIDEEEQSATRSMIDVNLVGVINGTQAAMKRMKQRGRGRIVNIASQAGKAGMAGGA
                     TYCATKFGVIGFSEAVRAELKGTGVSISWVLPGIVNTELAVGLPDIPLMDVLSPDDIA
                     GAVLEALTEGGSEIWVPARNQWLDAPVRLLPRSLREKIFALTGADKVLAEADSGTREE
                     YENRHSGVS"
     gene            4516..>5396
                     /locus_tag="P5138_01265"
     CDS             4516..>5396
                     /locus_tag="P5138_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRV59235.1"
                     /translation="MTEFAPSFATRDGLYDSTNEHDACGVAMVARLDNVPRHEVVSQG
                     LLALENLEHRGAAGADASTGDGAGILIQLPDVYYREEAGVDLPPAGQYGVAMCFLPRE
                     NEERREDLKTLISRMVETEGQIVLGWRDVPVDTEHVGNTADRSRPHVAQLFVGAGEGF
                     TEDQDAFERKLYVIRRICDKAAGGELYIASFSSRTIVYKGMLTSYQVPGFYPELKDER
                     VRSAMALVHSRFSTNTFPSWDLAHPFRLIAHNGEINTLRGNINWMRARESELASELFG
                     EDLEKVIPVVRPDGSDSA"
BASE COUNT         1005 a         1700 c         1812 g          879 t
ORIGIN      
        1 agcctgggtt tctccgctcg agtgggggga gggcatgacc acacatgtca cctgctccgg
       61 acctagtgca tcggcggcga gcgccgccac cagcgcggaa tcgatgccgc cggagagtcc
      121 cagcccgaca tggccgaacc cgttcttgac tacgtagtcg cggagaccgg tcaccagcgc
      181 cgtgtaaacc tcgtcgaggt cgtccatcca ttcggctacc ggcgccgacg cctcttccgg
      241 gtcatagacc agcaggtcct cagtgaactg tcctgccctt gcaaggacgc tgccgtccgg
      301 tccgatcagg gcgctcgagc cgtcaaagat cagttcgtcc tggccgccga ccatgttcac
      361 catcgctacc gggatgcctt tggccgtcgc cacccggcgg aagatttctt cccgctctag
      421 ccccttgccc cggtggtagg gagaggcgga ggaattgatg atcagctccg cgccgggggc
      481 cagatccgtg aagacggggg agcccggatc ccagcagtct tcgcagatgg tgaccccgag
      541 ccgggtaccg ccggcttccg tgaccatcgg ttcggttccg ttgcggaagg tccggtattc
      601 gtcgaagacg ccgtagttgg gcagcagtcg cttgcggtag acgcccctga tctcgccctg
      661 gctgatcacg gctgtggcat ttgcggcgat cgcccggccg accggattgc cgaccggttc
      721 cggaaagccg accagcgcgg tgatgtcccc gacccccgcg gccacctcgc gcaggcgctc
      781 gccggtggcg gcaatgaagt caggcctcag atacagatcc tcggccggat agccggtgag
      841 gaccaactcg gggaagacca cgatttcggc acccctctgc gcggcaaggt cgatccactc
      901 gctgaccagg gcggcgtttt cgtccagatc gccgacggtc gggttgacct gggccagggc
      961 gatcttgggc aacggactca cgggtctgag tatcccgttt tgcaggggtt cctgccgtgt
     1021 gaaggaacgc acacacaggt cgatccggct gccccagact cgtttgctca caaaacgcta
     1081 acaattagcg cggtccgcca ggttgcgggc ccgcgagaaa tgcgccgaac ttcccccgaa
     1141 gccggggaag gcggggaaac caaccgcggc tcactttgag ccgcatgggg tgaatccggt
     1201 ggtgatccgc cggtagtccg ggcccgggag gggcaggacg aatccgtcag ctaactccgc
     1261 aggcgcctca aaggagaaag tcaaagatga gttccagccg gattatccgt gccttgatgt
     1321 ccctgctcgc ctgtttcgcg gtgttcgccg cgctgggctc cagcgccacc gccaccacga
     1381 ccgcgggggc ttccagtgaa gccaacgcag tctccacggc caagtcggag gggaacgccc
     1441 gcaagggtcc cgtcgtgctg aacgtcgcgg attacagcgt caactacggc ggcaggttcc
     1501 gccttcgggg caagtccggc cgttcggtga agggaaatat ccgtattcag tcgaacaacg
     1561 gacacggctg gcaaaccctc cggaaagtcg ggaccgacgg cgagggtcgt tacgccgtga
     1621 aggtcgtcgc cagggtcaac acggcacttc gagccgtcgc cggtgacggc caccggtcaa
     1681 agatccgtcg cgtagcggtt atcggccagg tcaggctcga ccggccgaag cgttacgtga
     1741 agctcggcca gagtctgagg gtcagcggtg tcgtacttcc ccgtggttcc cggccaatcc
     1801 ggctgcgggt acgcggtcac ggggtcgtcg caaccaggtc ccgcgccaac ggacgcttca
     1861 cgcttcgttt caagccccgg agccacggcg acttcggcta ccgcgtctgg gctctgaaga
     1921 acgggctttc gaccggtgac agcagcctcg cccggcgttt cagcgccctg cggccgggcc
     1981 acgcttccta ctacgggccc ggcctctacg ggaatggcgt cgcctgcggc ggcaccctga
     2041 ccccgacgac ccgtggcgtg gccaacaagt acctgccctg cggcacgaag gtcacgctcc
     2101 agtacggcgg ccgtaccgtc gtcgcccggg tcatcgaccg cggcccttac gtggccggcc
     2161 gggactggga cctgaccgaa cagaccaaga aggacctcgg cttcggcggg gtcggagtgg
     2221 tctggaccaa caagtagccg tccggcaagc tctggcctga tcaaccggcc gggattcgtg
     2281 cccactcgcg gcgcgactcc cggccggatt gtttcttgaa gctcctgtgg catcatcggg
     2341 gcatgagttc ggccgaatcg aagaacctcg aggcgatctc gaaggcgatc gatcatcaca
     2401 acgccacctg cccgtttccg gcagcggagg tgcggatgaa tcccttcgag gtcgagcggc
     2461 tcggctggga ggaaattcgt ggcctgccga tcgttccgga cccgaacatc ggcaccggcc
     2521 gctttcacat cgtctgctcc cgggacatgg agggcgacga actcgaagag atcgaagcaa
     2581 tcggcgagga cctggtcacg gtcactcccg gcacccgcga agagcccgga gcacccgaag
     2641 ccgatcctgc gccggccgag aatcccggtc gctggaactg actagacggc gttccagaag
     2701 tcgatgccga ggtcggtcat ccagttctgg atctcgatgt tgatccggta gagctcgccg
     2761 gtccagatca ggatgcccat gatgatcagg atcacgccac cgattgcgat gatcagcccg
     2821 taatgtcgct tgattaccgc gaaggcagtg gtcatccggt tgaaggccag ggccgtgagc
     2881 aggaagggga tcgccaggcc ggccgaatag acggcaagca gcagggcacc gcgggctgcc
     2941 gaatcggtga gtgaggcggc cgagaggatg gcccccagag tcgggccgat gcagggcgtc
     3001 caggcaacgg caaaagctgc gccggccacc agcggcccgc ccttgccggc tctctgcatc
     3061 agcgcgtcga catgccattc gcggttcagc cgggtgacga aaaaggaggc cacgaagaga
     3121 atccccatgg cgatgatcag ggccgccgcg atcttgtcga ggaggtcgcg gtggtcggag
     3181 aggagcgaac cgatcttggt cgcgcccatc ccgagcagga tgaagaccgt cgagaaactg
     3241 gcgcagaaga tgagggcggg ggccaggacc ttgcgccagt cggcgcggtc gagatcgctc
     3301 acctgaactc ccgacacggc ggacaaatac ccgggaacga tgggcaaaac gcaaggagag
     3361 aggaaggaaa cgatgcccgc gaccagggcg gtcagcaggc cgactccggc ggcgggatcg
     3421 ctcgcggcaa aggggagggc cgccaggccc gcgtgaaatg tctcggcaaa cggcaaggga
     3481 aaacaggtta ctttggcggt tatggaacac gtcctggagg gtgcagttgt cgtggtaacg
     3541 ggtggagccg ccggaatcgg cagggccacc tcgacgaaga tggccgaggc gggggcccgg
     3601 gtcgcgatcg gcgacattca cggagagaag gccgcggagg cggccgagga ggtctcgaag
     3661 agggccgacg gcgccggttc cggaggagga gcgatcggaa agccccttga cgtcacggac
     3721 cgcgagggct tcgaactctt cctggacgac gtggaggccg aacttggccc gatcgactgt
     3781 ctggtcaata acgcgggggt gatgctgctc ggcccgattg acgaggagga gcagtccgcg
     3841 acccgttcga tgatcgatgt caacctggtc ggagtgatca atggaaccca ggccgcaatg
     3901 aagcggatga aacagcgcgg gcgggggagg atcgtcaaca tcgcctctca ggcaggcaag
     3961 gcgggcatgg ccgggggagc tacctattgc gcgaccaagt tcggcgtgat cggcttctcc
     4021 gaagcggtcc gggccgaact caagggcacc ggggtatcca tttcgtgggt gttgccgggc
     4081 atcgtcaaca ccgaactggc ggtcgggctg cccgatatcc ccctgatgga cgttctttcg
     4141 ccggacgaca tagccggcgc ggtgctggag gccctgacgg aagggggtag cgagatctgg
     4201 gtgccggcgc gcaaccagtg gcttgatgcc ccggtgcgtt tgctgccgag gtcactcagg
     4261 gagaagattt tcgccctgac gggtgccgac aaggttctgg ccgaggcgga ttcaggtaca
     4321 cgggaggagt acgaaaaccg tcattccggg gtctcctgag catcacggct gtttggcccg
     4381 ccggatagat gggagtgccc gggcttcggt aaactgcccc ggtgggctgg cccacctcgg
     4441 gcggcggttc cgaccgccgc caatacgtgt ctccaacaac aaattgccgc tctgcggcat
     4501 gaaaagcgcg cacaaatgac tgaatttgcc ccgtcgttcg cgacccgcga cggcctctac
     4561 gactcgacaa atgaacatga cgcctgtggc gtcgccatgg tcgcgcgcct cgacaacgtg
     4621 ccccgtcacg aagtcgtcag ccagggtctc ctcgctcttg agaacctcga gcaccgcggt
     4681 gcggcgggtg ccgatgcctc gaccggtgac ggcgccggca tcctgatcca gctgcccgac
     4741 gtctattacc gggaagaggc tggcgtggat ctgcctccgg ccggccagta cggcgtggcc
     4801 atgtgcttcc tgcccaggga gaacgaggaa cggcgggaag atctcaagac tctgatctcc
     4861 aggatggtgg agaccgaagg gcagatcgtg ctcggctggc gggacgtccc ggtcgacacc
     4921 gagcacgtag gcaacacggc cgaccgttcc cgtccccatg tggcccagct ctttgtcggc
     4981 gccggcgagg gattcaccga agaccaggac gcattcgagc gcaagctcta cgtgatccgg
     5041 cgaatctgcg acaaggccgc tggcggcgag ctgtacatag cgtcgttttc gtcccggacc
     5101 atcgtctaca agggcatgct cacctcgtac caggtgccgg gtttctatcc ggagctcaaa
     5161 gacgaacggg tccgctcggc gatggcactc gtccattcgc gcttctccac gaacaccttt
     5221 cccagctggg acctggccca tccgttccgg ctgatcgccc acaacggcga aatcaacacc
     5281 ctgcgcggca acatcaactg gatgcgggcc cgcgagtccg aactcgcatc ggaactgttc
     5341 ggcgaagacc tcgagaaggt gatcccggtg gtccggcccg acggatcgga ctcagc
//
LOCUS       DAOTIP010000036         5378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_18092_length_5378_cov_3.015593, whole genome shotgun sequence.
ACCESSION   DAOTIP010000036 DAOTIP010000000
VERSION     DAOTIP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18092_length_5378_cov_3.015593"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="P5138_01270"
     CDS             <1..1110
                     /locus_tag="P5138_01270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV59236.1"
                     /translation="DQNPIQSGRLSQKGKSLIVQIRTSQKVNMGKLDRRPDFMGGNHR
                     YLCLQMNPRGGTSFVRICLGGPTRTYSVLGVSRTSDTGRVRSSKVIRAEVKKATPYRL
                     VATFDPRAAGLPSGKYAWRIANHSGRCPQSGPDARPIGECLSFFPPKRRAVYDLRPVR
                     VVGCTGGNGEFVTHGPRGRKRVALTFDDGPSDYTPKVLHILKRKKASATFFMLGQQVA
                     RYKTFARRALALGHEIGNHSYDHDLLPSSSNLRHANRTISQVTHFRPCLFRPPYGAVN
                     SSLKASARAERMKVINWDVDTNDWKLPGSSAIRSTILGNVRPGSIVLMHDGGGPRQGT
                     VDALSGAISGLRARGYRLVTVSQLLGNRMIYRPVP"
     gene            complement(1207..3675)
                     /locus_tag="P5138_01275"
     CDS             complement(1207..3675)
                     /locus_tag="P5138_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59237.1"
                     /translation="MRRYAVLLVALIAGAVLTGGVSPASGKKPKTVKPTVKVVNLKAS
                     QLARGKVKVLVKANRKAKVRLSATTYTFDTGNKPLFKSRTVKLGKKKSRVVTIKLNPA
                     VKSGMSSCQLRKLTLKARNGKRKSSRTQTVVRDQSDCKLQPVDLSRAEDCDFIAEPKE
                     GLCMLPFPDDYYTVKDSSSPTGKRIHFTDAAMPRNKNDIPVKASNYDPSDGFSQGQGI
                     VVKIPGIESQEAVDANGFVPINHIGDYAEPDQKAVVINADTGERHPIWVNIDANALQD
                     SDRALEIKPAVNFDEKGHYIVALRNLVDEDGNALAAPNAFRYYRDQVKSGEPEIESRR
                     AHFETIFKDLKKAGIKRSSLYLAWDFTTASNENNYKRVLSMRDRAFAELGDTTMGDQI
                     VQGDAPDFHIDSVVNYTEGQNSQIARKVTGTFEVPCFLSPSCVPGSTMELDSNGLPTE
                     HGTYTANMVCVIPRVGLDGPDPVKTRPMVFGHGLFGDATGVDGGPNPPLAQHGITICA
                     TDEIGMSSSDVAQVSIQALPDLSKFPVLADRLAQAILNELYLARLMYHPDGLGSVPAF
                     QDGDGYVPGDSVIKTDHVYYMGASQGGILGGALTAVSPDFVQSSLLVGAMNYSIMLPR
                     SIDFDIYGGILYSFYKNDLEHPLLFQLMQMLWDRSEPNGFAHVMTDNPPPNTPEHRVT
                     LQIALGDHQVSNYASEVMARTLGMKTNPVPIDDGRWPDYDALWNVPRLAASDFPYHGN
                     NIIYFDGGPPRPDPNNPSKTIGTDPAPFANVPNRVAQDPHGAPGGASVAVALTSSFLQ
                     PNGYIDDVCSPHACYGDAWDGSLP"
     gene            3847..5061
                     /locus_tag="P5138_01280"
     CDS             3847..5061
                     /locus_tag="P5138_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRV59238.1"
                     /translation="MTSTFPRRIGLLALLLPVLFIAIQSQIPRAEAGLTVPSAPKRGV
                     ANPFARAGMWIWYIDSSNRGNLGSIIKQARRAKIGTLYIKSGDGTDAWSQFNKPVIRR
                     FHKAGLKVCGWQYVYGRRPIAEAKISAAAKRRGADCFVIDAEAEYEGRYASADWYIRR
                     LRALVGSRFPIGLSSFPYVNYHPGFPYSVFMGPGAATANVPQVYWHTIGDSVRASLEI
                     TWEQNSVYKRRIYPVGQTYENPGSRELLAFRRFMLNYGTAPSWWSWQETNSAEWSALS
                     KRVARIPGYRAYAGHVTLKKGDRGDQVVWLQQHLIAAGYSMEPTGIFSNVTYRAVRKF
                     QARRGLKADGVVGTRTWNYIMRVRPVRVRWSAAKRRSRSAGASASSAPLKAPLSAGLP
                     MVRNELAGAKHP"
     gene            complement(5068..>5378)
                     /locus_tag="P5138_01285"
     CDS             complement(5068..>5378)
                     /locus_tag="P5138_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRV59239.1"
                     /translation="SAPCGPALVTPDEAGPHDAIGISMLVNGEERQSSSTSDLIFSVP
                     RLVSTLSTWMTLEPGDIIATGTPSGVGLAMDPRTWLEDGDELIVRSPELGELRTHIRR
                     "
BASE COUNT          883 a         1721 c         1786 g          988 t
ORIGIN      
        1 gaccagaacc cgatccagtc gggccgtctg agccagaagg ggaagtccct gatcgtccag
       61 atcaggacca gccagaaggt gaacatggga aagctcgacc ggcgacccga cttcatgggc
      121 ggcaaccacc gctacctctg cctgcagatg aatccccgcg gcggcacgag cttcgtgcgg
      181 atctgcctcg gtggcccgac ccggacctac agcgtgctcg gtgtctcccg cacttccgac
      241 accggccggg tccggtccag caaggtgatc cgtgccgaag tcaagaaggc aactccgtac
      301 aggctcgtcg ccaccttcga tccacgtgcg gccgggttgc cgtccggcaa gtacgcgtgg
      361 aggatcgcca atcattccgg tcgatgcccc cagtccgggc cggacgccag gccgatcggc
      421 gaatgcctct ccttcttccc gccgaagcgc cgcgccgtct atgacctgcg gccggtccgc
      481 gtggtcggct gcaccggcgg caacggcgag ttcgtgactc acggtccgcg gggccgcaag
      541 cgggttgcgc tgaccttcga cgatggcccc agcgactaca cgccgaaggt ccttcacatc
      601 cttaaacgga agaaggcgag cgctaccttc ttcatgctcg gccagcaggt tgcccgctac
      661 aagacttttg cccgccgggc gctggccctc ggccacgaga tcggcaacca cagctacgac
      721 catgacctgc tccccagcag ttccaacctc cggcatgcga accgaaccat cagccaggtc
      781 acccatttcc ggccctgcct cttccgcccg ccctatggcg cggtcaactc ctcgctcaag
      841 gcatcggccc gggccgaacg gatgaaggtg atcaactggg acgtcgacac caacgactgg
      901 aagctgcccg gctcctcggc gatcaggtcg accatcctgg gcaacgtgcg gcccggctcg
      961 atcgtcctca tgcacgacgg gggcggaccg cggcagggca ccgtggacgc actctccggc
     1021 gcgatcagcg gcctgcgggc gcgcggctac aggctggtca cggtttccca gttgctgggc
     1081 aaccggatga tctaccgccc ggtgccctga cggtcccgga tcgtcgcccg gaaaagcgcg
     1141 aaatccggaa agcggccgcc ggggccactc tccggatctc gttgaaccgc gaccggctgc
     1201 aggggcttac ggcaggctgc cgtcccaggc gtcgccgtag caggcatgcg gcgagcagac
     1261 gtcgtcgatg tagccgttcg gctgcaggaa cgacgaggtc agggcgaccg ccacgcttgc
     1321 tccgccgggg gctccgtgcg gatcctgggc gacccggttc gggacgtttg cgaacggtgc
     1381 cgggtcggtt ccgatcgtct tcgacgggtt gttcggatcg ggccggggcg gtccgccgtc
     1441 gaaatagatg atgttgttgc cgtggtacgg gaagtcgctg gcggccagac gcggcacgtt
     1501 ccagagcgcg tcgtagtcgg gccagcgtcc gtcgtcgatc ggtaccggat tcgtcttcat
     1561 gcccagggtc cgggccatca cctcggaggc gtagttggac acctgatgat caccgagcgc
     1621 gatctgcagg gtcacgcggt gttccggtgt gttcggcggc gggttgtccg tcatcacatg
     1681 ggcgaagccg ttcggctcgc tgcggtccca gagcatctgc atcagttgga agagcagcgg
     1741 gtgttccagg tcgttcttgt agaacgagta gaggatcccg ccgtagatgt cgaagtcgat
     1801 cgagcggggc agcatgatcg agtagttcat cgccccgacc agcagcgagg actggacgaa
     1861 gtccggtgag accgcggtca gcgcaccgcc gaggattccg ccctggctgg cgcccatgta
     1921 atagacgtgg tcggtcttga taaccgaatc gcccgggacg tagccgtcac cgtcctggaa
     1981 ggccgggacc gagccgagtc cgtccgggtg gtacatgagg cgggccaggt acagctcgtt
     2041 gagaattgcc tgggccagcc ggtcggcgag cacggggaac ttggacaggt ccggcagggc
     2101 ctggattgac acctgcgcca catcgctcga ggacatgccg atctcgtcgg tggcgcagat
     2161 ggttatgccg tgctgggcga gcggcgggtt ggggccgccg tcaacaccgg tggcgtctcc
     2221 gaacaggccg tggccgaaga ccatcggcct ggtcttgacc gggtccggtc cgtcgaggcc
     2281 tacgcgcggg atgacacaga ccatgtttgc ggtgtaggtc ccgtgctcgg ttggcagccc
     2341 gttggagtcg agttccatgg tgctgcccgg gacgcagctc ggggaaagga agcagggcac
     2401 ctcgaaggtc ccggtgacct tgcgggcgat ctgcgagttc tgcccctcgg tgtaattgac
     2461 caccgaatcg atgtggaagt ccggggcgtc accctggacg atctggtcgc ccatggtggt
     2521 gtcaccgagc tccgcgaagg cccggtcacg catgctcagg acgcgcttgt agttgttttc
     2581 gttgctggcc gtggtgaaat cccaggccag gtagagactg ctgcgcttga tgcctgcctt
     2641 cttcagatcc ttgaagatcg tctcgaagtg ggccctacga ctttcgatct ccggctcacc
     2701 ggacttgacc tggtcccggt agtagcggaa ggcgttcgga gctgcgagcg cgtttccgtc
     2761 ctcgtcgacg agattgcgca gggcgacgat gtagtggccc ttctcgtcga agttcacggc
     2821 cggcttgatc tccagggcac gatccgagtc ctgcagggcg ttggcgtcaa tgttgaccca
     2881 gatcggatgc cgctctccgg tgtcggcatt gatcacgacc gccttctggt ccggctcggc
     2941 gtagtcgccg atgtggttga tcggtacgaa gccgttcgca tcgaccgcct cctgcgattc
     3001 gatcccggga atcttcacca cgatgccctg gccctggctg aagccatcgg acgggtcgta
     3061 attggaagcc ttgaccggaa tgtcgttctt gttgcggggc atcgccgcgt cggtgaagtg
     3121 gatccgcttg ccggtcgggc tggaggagtc cttcaccgtg tagtagtcgt ccgggaaagg
     3181 cagcatgcac aggccttcct tcggttcggc gatgaagtcg cagtcctcgg cccggctcag
     3241 atcaacaggc tggagcttgc agtcggactg atcccggacc acggtctggg tgcggcttga
     3301 cttgcgcttg ccattgcggg ccttgagcgt gagcttgcgg agctgacagc tgctcatgcc
     3361 ggacttgacg gccggattca gcttgatggt cacgacccgg ctcttcttct tgcccagctt
     3421 gaccgtgcgc gacttgaaca gcggcttgtt gccggtatcg aaggtgtacg tcgtggccga
     3481 gagccgcacc tttgccttcc ggttcgcctt gaccaggacc ttcaccttgc cgcgggccag
     3541 ctggcttgcc ttgaggttga cgaccttgac cgtcggcttg acggttttcg gcttcttgcc
     3601 cgaggccggg ctgactccgc cagtgagaac ggctccggca atcagagcga ccagcagaac
     3661 ggcgtaacga cgcatccttg attcctccca aacagaaatt gaacttctcc ctgccccgca
     3721 ggctaacagg gcaagggccg gaattgaaag aaagcttgct cccggccggt tgatcgcggt
     3781 tcgggcgggt atctcctgcg aggctgaatc cgcaccaatt cgagccgaga ggtgttccag
     3841 aaactgatga catcgacttt cccccggcgc atcgggctgc tcgccctgct gcttccggtc
     3901 cttttcatcg caatccagtc gcagattccc agggccgagg ccggcctgac ggttccctct
     3961 gccccgaagc ggggagtcgc caaccccttt gcccgggcgg gaatgtggat ctggtacatc
     4021 gactcttcca accgggggaa cctcggctcg atcatcaagc aggccaggcg ggcgaagatc
     4081 ggcacgctct acatcaagtc cggggatggc accgatgcct ggagccagtt caacaagccg
     4141 gtcatccgcc gcttccacaa ggcgggcctc aaggtatgcg gctggcagta cgtctacggc
     4201 aggcggccga tcgccgaggc gaagatttcc gccgccgcca agcggcgcgg ggcggactgc
     4261 ttcgtgatcg acgcggaagc cgagtacgag ggccgctatg cgtcggccga ctggtacatc
     4321 cgcaggctgc gtgccctggt tggcagcagg tttccgatcg gcctctcctc gttcccgtat
     4381 gtgaactatc acccgggctt tccctattcg gtcttcatgg gccccggcgc cgcgaccgcg
     4441 aatgtgccgc aggtctactg gcacacgatc ggtgactcgg tccgggcctc gctggagatc
     4501 acctgggagc agaacagcgt ttacaagcgg cggatctatc cggtcggcca gacctacgag
     4561 aaccccggca gccgggagct cctcgccttc cgccggttca tgctcaatta cgggacggca
     4621 ccgagctggt ggtcctggca ggagaccaat tccgccgaat ggtcagccct ctcgaagcgg
     4681 gtcgcccgga tccccgggta ccgggcctac gccggccatg tcaccctcaa gaagggcgat
     4741 cgtggagacc aggtggtctg gctgcagcag catctgatcg cggccggcta ttcgatggag
     4801 ccgaccggca tcttctcgaa cgtcacttac agggccgtgc gcaagttcca ggcgaggcgg
     4861 ggtctgaagg cggacggcgt ggtcggaacc cgcacctgga actacatcat gcgggtccgg
     4921 ccggtccggg tccggtggtc ggccgcaaaa cgccgcagcc gctccgctgg cgcctcggcc
     4981 tcatcggcac cgctcaaggc cccgctttcg gccggcctcc cgatggtcag aaacgaactg
     5041 gccggcgcga agcacccctg atccgggtca acgcctgatg tgagtccgga gttcacccag
     5101 ctcgggcgag cggacgatga gctcgtctcc gtcttcgagc caggttcgcg ggtccatcgc
     5161 gaggccgact ccggagggcg tgccggtcgc gatgatgtct cccggctcga gggtcatcca
     5221 ggtcgaaagg gtcgagacga gccggggcac ggagaagatc aggtcgctcg tcgacgagct
     5281 ctgtcgctct tcgccgttga ccagcatcga gatgccgatc gcgtcgtggg ggcctgcctc
     5341 gtccggggtg accagggcgg ggccgcaggg ggcggagc
//
LOCUS       DAOTIP010000037         5358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_18188_length_5358_cov_3.376579, whole genome shotgun sequence.
ACCESSION   DAOTIP010000037 DAOTIP010000000
VERSION     DAOTIP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18188_length_5358_cov_3.376579"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="P5138_01290"
     CDS             <1..292
                     /locus_tag="P5138_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV59240.1"
                     /translation="CLEIDAARLLVWRASWMGRNQIPFKNSEGSMSKLKGGEVATWVT
                     ERAIQILGGYGYTREYPVERMHRDAKIYDIFEGTAEIQRLVISRSISGLKIK"
     gene            481..1839
                     /locus_tag="P5138_01295"
     CDS             481..1839
                     /locus_tag="P5138_01295"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HRV59241.1"
                     /translation="MNSSGQTIAETFFTQPAPDGKLPLNGVNARSTMRVLESEMMLDG
                     DPDKNLATFVTTFMEEEARMVIAQNLHRNYIDHAEYPRTAEISKRCVRMIHSLFHGEE
                     GEESPGTATAGSSEAVMLGALAMKWQWKRRRKEAGKPDDKPNLVYGSDVHVVWDKFCR
                     YFDVEPRQANVPKGKTTVGPEELGPLIDENTIGVIAVVGTTFTGECDDVEGLDRMLGE
                     LKGKGLEVPMHIDAASGGFVFPFSHPAFEWDFRLDSVVSINTSGHKFGLVYPGIGWLL
                     FRNEAQLPEDLVFYEDYLGEKDATFTLNFSGSASFVLAQYYMFIRLGYNGYSSIVRAM
                     TENRHSLADQLKKEDALEVFDSDRPTLPLVICKVREGENFDASHLVGELARRRGWLVP
                     AYDMPPDNEDQKIIRMLVKVNQTRELVDALVQDFHDSIGLLRDTDLSKVGDLPVHSGT
                     GY"
     gene            1904..3322
                     /gene="gltX"
                     /locus_tag="P5138_01300"
     CDS             1904..3322
                     /gene="gltX"
                     /locus_tag="P5138_01300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRV59242.1"
                     /translation="MSNESELKVRFAPSPTGALHIGGARTALYNWLAARHGGGQMVLR
                     IEDTDLERSTPENVAQILDALRWLDLDWDEGPISQLGRAEAHAAALRKLLDSGAAYRD
                     SATADDVKAWKVEHGSDRGYRGTPSEDGSGAIRLRVPDDGDTVVDDLIRGEVRFPNRS
                     YDDFVIARGDGSVLYNFAVAVDDADMGITEVIRGDDHLSNTPKQLMVFEALGIEPPRY
                     AHVPLLHGPDGKKLSKRHGAASVQELRDAGYLPEAIRNYVALLGWGPGDDETLLTTAE
                     LIERFRPEDVGRSSAVFDEKKLRWMNGRYMRDMPIEDYRSAVRSFVDAETAGRPLNEE
                     RFRFGCEIVQEKAQTLQEVWPLIRFLFEPPVQDDKAWRKVMKGDAERVLTEVEAVLID
                     LPQGEEGPNRDGFSIGAVQAVLEPLPEKLDLGAGKVFQPIRVAITGTTISPGIFESVA
                     ALGREETLARVEAARSKLRDQD"
     gene            3428..3826
                     /locus_tag="P5138_01305"
     CDS             3428..3826
                     /locus_tag="P5138_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV59243.1"
                     /translation="MISVTSKSRYAVVALVELAGAGETPMPVKELAERRNIPDQFLEQ
                     LFSTLRRAGLLTSRRGSKGGYILSRPANEITVLEVVQALDGKVGQEADEAGGIWAEGV
                     RALRGVFGETTIEDLYERESQDAAARMYFI"
     gene            3823..4743
                     /gene="cysK"
                     /locus_tag="P5138_01310"
     CDS             3823..4743
                     /gene="cysK"
                     /locus_tag="P5138_01310"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRV59244.1"
                     /translation="MIAPQASATVGGTPLVWLARIGDGLDAQICGKLEYFNPGGSVKD
                     RIGAAMVGAAEARGELVRGESVIIEPTSGNTGIALAMICAARGYELILTMPEGMSRER
                     TKLLRAYGAEVRETSSMGGMDEAVELARKIASERDGYMPLQFSNPANPEAHFRSTGPE
                     VWTDTGGDIDAFVCGVGTGGTITGVARYLKEQGSDARIYAVEPASSPVLSGGRVGPHR
                     IQGIGAGFVPEVLDMDLVDEVIPVSDDDALRTARKLASTEGILGGISAGANVHAALQL
                     AGRPELAGGRIVTVICDSGDRYMSLPFFAT"
     gene            4773..>5358
                     /gene="cysE"
                     /locus_tag="P5138_01315"
     CDS             4773..>5358
                     /gene="cysE"
                     /locus_tag="P5138_01315"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRV59245.1"
                     /translation="MADDLISRTFGGLRSHIAAARGRDPATANVSSLEIILTWPGVQA
                     VLAYRLANRLRRSAIPLLPFAISYLSRAITGIEIHPDARIGPGLFIDHGSGVVIGETA
                     EIGENVTLYQGVTLGGTGFQSGKRHPTVEDYVTIGSGAKLLGPITVGHGAKIGANSVV
                     VEDVPPNSTVVGNPGHPVRVEGRKVTDSPDADWIH"
BASE COUNT         1008 a         1675 c         1785 g          890 t
ORIGIN      
        1 gtgcctcgag atcgacgccg cccgtctgct cgtctggcgt gcctcctgga tgggacgcaa
       61 ccagatcccg ttcaagaact ccgagggttc gatgtcgaag ctcaagggcg gcgaggtcgc
      121 tacctgggta accgagcgcg ccatccagat cctcggcggc tacggctaca cccgcgagta
      181 cccggtcgag cgcatgcacc gcgacgcgaa gatctacgac atcttcgagg gcacggcgga
      241 gatccagcgc ctggtcatct cccgcagcat ctcgggactg aagatcaagt agccagttca
      301 gcacctctgg tgccgaaagg cccgtcccac taggggcggg cctttttctt tggttccgca
      361 caccggaact tcacccggac ggggtgaggg gggatgtagc gttcagggag aaaatagtcc
      421 ggtacgccac tttccctgga ggaccacttg cctgatcaac ccgatgtctt tgcgcctgtt
      481 gtgaattctt ccggtcagac gatcgccgaa acattcttca cccagccggc gcccgacgga
      541 aagctgccgc tgaatggggt caacgcacgt tcgacgatga gggtcctgga gagcgagatg
      601 atgctcgacg gcgatcccga caagaacctc gccaccttcg tgaccacttt catggaggaa
      661 gaggcgcgca tggtgatcgc ccagaacctg caccggaact acatcgacca cgccgagtac
      721 ccccgcacgg cggagatctc gaagcggtgc gtacggatga tccacagcct cttccacggg
      781 gaggaaggcg aagagtcgcc cggaaccgcg actgccggct catcggaggc cgtgatgctg
      841 ggcgcactgg cgatgaaatg gcagtggaag aggcggcgca aggaagccgg caagccggat
      901 gacaagccga acctcgttta cgggtcggac gttcacgtgg tctgggacaa gttctgccgc
      961 tacttcgacg tggaacccag gcaggccaac gtgcccaagg gcaagacgac cgtcggcccg
     1021 gaggagctgg gtcccctgat cgacgagaac acgatcggcg taatcgccgt ggtcgggacc
     1081 actttcaccg gggaatgcga tgacgtcgag ggtctcgacc ggatgctcgg tgaactcaaa
     1141 ggcaaggggc tcgaggtgcc gatgcacatc gacgcggcca gcggcggttt cgtctttccc
     1201 ttctcgcacc ctgccttcga atgggatttc cgactcgatt cggtcgtctc gatcaacacc
     1261 tccggtcaca agttcgggct ggtctatccg ggaatcggct ggctgctgtt ccggaacgag
     1321 gcccaactgc cggaagatct cgtcttctac gaggactacc tcggtgagaa ggacgccacc
     1381 ttcaccctca acttctccgg tagcgcatcg ttcgtgctgg cccagtacta catgttcatc
     1441 cgcctcggct acaacggtta ttcctcgatc gtgagggcga tgacggagaa ccggcactcc
     1501 ctggccgacc agctgaagaa agaagacgca ctcgaggtgt tcgactcgga ccgcccgacc
     1561 ctgccgctgg tgatctgcaa ggtcagggaa ggcgagaact tcgacgcaag tcacctggtc
     1621 ggagagctgg ccaggcgccg gggctggctg gttccggcat acgacatgcc gcctgacaac
     1681 gaggaccaga agatcatccg gatgctggtc aaggtcaacc agacccggga actggtcgac
     1741 gccctcgtgc aggacttcca cgattcgatc ggactgctgc gggacaccga cctgagcaag
     1801 gtgggcgatc taccggttca ctccggaacg ggctactagc gtctgtctcc gaggtgaacc
     1861 ggcgtgatgc cggtgtcgcc gccgctgtgg aaacatggcg gcagtgagca acgaaagtga
     1921 attgaaagtc agattcgcgc cttccccgac cggggcgctt catatcgggg gagccaggac
     1981 tgccctctac aactggctcg cggcccggca cggcggcggc cagatggtcc tgcgaatcga
     2041 ggacacagac ctcgaacgat cgaccccgga gaatgtggcc cagattctcg atgccctgcg
     2101 ctggctggac ctggattggg acgagggccc gatctcccag ctgggccggg ccgaagcgca
     2161 tgcggccgcg cttcggaagc tgctcgattc cggtgctgcc tacagggact cggccaccgc
     2221 cgatgacgtc aaggcctgga aagtcgaaca cggctccgat cgcggatacc ggggcactcc
     2281 ctcggaagac ggaagcgggg cgatccgtct gcgggttccc gacgacggtg acacggtcgt
     2341 agacgacctg atcaggggcg aggtcaggtt cccgaaccgc agctacgacg acttcgtgat
     2401 cgcccgcggc gacggctcgg tgctctacaa cttcgcggtc gcagtggatg acgcagacat
     2461 ggggatcacc gaggtcatcc gtggcgacga tcacctctcc aataccccga agcagttgat
     2521 ggtcttcgag gctctgggta tcgaaccacc ccgctacgcc cacgtgccgc tgcttcacgg
     2581 tccggacggc aagaaactct cgaagcggca tggcgcggcc tccgtccagg aactccgcga
     2641 tgccggatac ctgccggagg cgattcgcaa ttacgtcgcc ctgcttggct ggggtccggg
     2701 cgatgacgag actttgctca ccacggcgga gctgatcgaa cggttccggc cggaggacgt
     2761 gggcagatcg tcggcagtct tcgacgagaa gaagctcagg tggatgaacg ggcgctatat
     2821 gcgcgacatg ccgatcgagg actaccggag tgccgttcga agcttcgtcg atgcggagac
     2881 cgccgggagg ccgctgaatg aagagcgctt ccggttcggt tgcgagattg tgcaggagaa
     2941 ggcccagacc ctgcaggaag tctggccgct gatccgcttt cttttcgagc cgccggtcca
     3001 ggacgacaag gcctggcgca aggtgatgaa gggcgacgcc gagcgggtac tgaccgaggt
     3061 cgaggcggtg ctgatcgacc tgccgcaggg ggaggagggt cccaaccggg acggcttctc
     3121 gatcggggcc gtccaggcgg ttctggaacc cctgccggaa aagctggacc tgggagcggg
     3181 aaaggtcttc cagccgatcc gggtggcgat caccggaacg accatttcgc ccggcatctt
     3241 cgagtcggta gcggccctcg gacgcgaaga gaccctggcc agagtcgaag cggcgagatc
     3301 gaaactgcgg gaccaggact gacagtcgct cgattcggac cggaccctcc gggcccgatt
     3361 tcctgaaata cctgttttct gacagggtta gtctgctttc tgccgctcca tgcggtaaaa
     3421 aggtgtcgtg atctcggtta cctcgaaatc gcgatacgcc gtcgtggcgc tggtcgagct
     3481 ggccggagcc ggagagactc cgatgccggt caaggaactg gccgagcgcc gcaacattcc
     3541 cgatcagttc ctcgagcaac tcttctcgac cctgcggcgg gccggtctcc tgaccagccg
     3601 ccggggcagc aagggcggct acatactttc ccgtcccgcc aacgagatca ccgttctcga
     3661 agtcgttcag gcgctggacg gaaaggtcgg ccaggaagcc gacgaggccg gcggaatctg
     3721 ggccgaaggc gtccgcgcgc tgaggggggt gttcggggaa accacgatcg aggatctcta
     3781 cgagcgcgaa tcgcaggacg ccgcggcgag gatgtacttc atttgatcgc gccacaggca
     3841 tcggcgacgg tgggtggcac cccgctggtc tggctggccc ggatcggcga cgggctcgac
     3901 gcgcagatct gcgggaaact cgagtacttc aatcccgggg ggtcggtcaa ggaccggatc
     3961 ggtgcggcaa tggtcggcgc ggccgaggcg cggggggaac tggttcgcgg cgagtcggtg
     4021 atcatcgagc cgaccagcgg caacaccggc atcgccctgg ccatgatctg cgccgcgcgg
     4081 ggctacgaac tgatcctgac catgccggag ggaatgagtc gcgagcggac caagctgctg
     4141 cgtgcctacg gagccgaggt cagggaaacc tcttcgatgg gcgggatgga cgaggctgta
     4201 gaactggcca ggaagatcgc gagcgaacgg gacgggtaca tgccgctcca gttctccaat
     4261 ccggccaacc cggaggcgca cttccgctcg accgggccgg aggtctggac cgacaccggg
     4321 ggtgacatcg acgccttcgt ctgcggggtc ggcaccggcg ggacgatcac cggggtggcg
     4381 cggtacctga aggagcaggg ctccgacgcc cggatatatg cagtcgaacc ggcctcctcg
     4441 ccggtccttt ccgggggaag ggtcggcccg caccggatcc agggaatcgg cgccggtttc
     4501 gtgcccgagg tgctggacat ggatctggtc gacgaggtga tcccggtcag cgacgacgac
     4561 gccctgcgta cagcgcggaa gctcgcctcg accgagggaa tactgggcgg gatatcggcc
     4621 ggcgccaatg tccacgcggc cctgcagctg gccgggcgac ccgaactcgc cggcggcagg
     4681 atcgtcacgg ttatctgcga cagcggcgac cgatacatgt cacttccctt cttcgcaacc
     4741 tgacccgagc cgcgaccttc taggctcttc cggtggccga tgacctgatc agccggacct
     4801 tcgggggctt gcgctcccac atcgccgctg cccgtggcag ggatccggcg acggcgaacg
     4861 tgtccagcct ggagatcatc ctcacctggc cgggtgtcca ggcggtgctc gcctaccgcc
     4921 tcgccaaccg cttgcgccgc tcggcgatcc cgctgcttcc cttcgcgatc tcctatctca
     4981 gccgggcgat caccggcatc gaaatacacc ccgacgcccg gatcggaccg ggcctcttca
     5041 tcgaccacgg ctccggggtg gtcatcgggg aaaccgccga gatcggcgag aatgtgaccc
     5101 tctatcaggg cgtgaccctg ggaggaaccg gatttcagtc cggaaagcgg cacccgaccg
     5161 tcgaggacta cgtgacgatc ggctccggag cgaagctgct cggtccgatc acggtcggcc
     5221 acggcgcgaa gatcggggcg aattccgtgg tggtcgaaga cgtaccacca aattcgaccg
     5281 tggtcggcaa cccgggtcat ccggtccggg tcgagggccg caaggtgacc gacagcccgg
     5341 acgccgactg gatccacc
//
LOCUS       DAOTIP010000038         5302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_18451_length_5302_cov_3.368973, whole genome shotgun sequence.
ACCESSION   DAOTIP010000038 DAOTIP010000000
VERSION     DAOTIP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18451_length_5302_cov_3.368973"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="P5138_01320"
     CDS             complement(<1..674)
                     /locus_tag="P5138_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRV59246.1"
                     /translation="MQALVLVGGKGTRLRPLTYETPKPALTLVDRPFLTYMTEWLGSH
                     GVDEVILACGFLPDEMKKVLGDGEPGGPRLTYLVEEDTLGTAGAIRYALDLLDDRFFA
                     LNGDVLADLDLTALWKQHEETGARATLGLYEVEDSSSYGLVDIDGNGQVLQFSEKDPE
                     RTGTGLINAGTYVLEREAIAEIPAGREVSIETEFYPSITGKGLYARPLGGYWMDFGTR
                     ERYFDA"
     gene            complement(694..1986)
                     /locus_tag="P5138_01325"
     CDS             complement(694..1986)
                     /locus_tag="P5138_01325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRV59247.1"
                     /translation="MNSEEKAPIGVIGAGWVGLVTAACFAELGHRVIARDVVQEKVDS
                     LSRGEITIHEPGLAELIENNRDRLTFTTDMGELLDSARLLFVCVDTPPTYSGDADLSR
                     VRSVVEDLKANGDHVLIMKSTVPAGTGASIRRELPGLAYVSCPEFLKEGTAVEDFMHP
                     DRVVVGSEPEDSAAAEEVADLYRPLGGEFVMTDTSSAEMIKLASNAYLATRISFINEI
                     ANVCEEVGADVGEVARGMGLDERIGPHFLRPGIGFGGSCFPKDVNALKLLAGNSGYHF
                     QLLNSVIEVNELQKRRVVSKLTKHLGSLVGKRIALLGLAFKPDTDDMREASSLVLSAR
                     LRGEGAEVVAYDPVAMSNARSLLPGVEMSRTAQEALADSDAAVLVTEWSEFKEIDWTE
                     AADRMRRPLLIDGRNFLDAGKLRSAGFLYEGIGKAGDR"
     gene            complement(1992..4199)
                     /locus_tag="P5138_01330"
     CDS             complement(1992..4199)
                     /locus_tag="P5138_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRV59248.1"
                     /translation="MTEEHEEPEDKEGVAPEGGEYPPAQPAPKAGGSQEDSPAEKPGE
                     YVEPPTEELFAFEARVEEGEVPDLDPVFRAALLEGSESAVSATPAEGKSAPEGETAPE
                     AGQEAAPVAASAGEGGDKVEGGQEASAGEESATGEEPLTGEEAHTGEEPLTGEEPATG
                     TQEWEQPSETGITAEQTAVLQASARTEVHDAIDYSRRTGNVWDPTRPPTGGDFDKPGK
                     RPHYWWRFLLAVVVIIGSFATATQASVMHIVNDIGTKIGEGENKIALPPYALKDADGP
                     QTILIIGSDVRTGGGDTAGDPGRSDTAMLVRLDPDTDQIAMLSIPRDLYTDIPGYGYD
                     KFNAAYTYGGTALTLKTIKSITGLEVNHVVNIDFQGFADVIDAIGCVYVDVDRHYFND
                     NAGLSVSEQYATIDIEAGYQKLCGADALSFARYRHTDSDLVRAARQQDLIGEVRNRLT
                     LGEVWKRRDELLSAFTDNTRSDISGGDQIIQLLALMFDSRGAAVTEVSFPSTFTMRDG
                     ISYVEATPDEIDAAVNKFLGFEDQPGPVGRLDEGDGANNKARKKAQAREEKQAARERK
                     KNLEDSTSKIPNKNNDDLVDAADSGLSVAQDLDSQITRTDFPVYYPKRLPSGAIYADG
                     QSRVYHTRDPDKNVYGAYRMVMEVQLDDGLHYFGLQGLRGWTDPPILDAPHDEVEMDD
                     RKFQVYPEGDRIKLVSWTDGDTVYWISNSLLLGLDNQQMLGMARSTRAFTPNK"
     gene            complement(4196..>5302)
                     /locus_tag="P5138_01335"
     CDS             complement(4196..>5302)
                     /locus_tag="P5138_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV59249.1"
                     /translation="HLIGYDTSHEEGVHEAAGFIKGWLDSRGVETEQGVCRGLPVITA
                     SRGEPDAPTVILHGHIDVVPAEAGQFEARVEGDRLIGRGAYDMKAALAAMMLALPTAE
                     EPVPGVRVVLGIVSDEESEEADNRGSNYLVRNGQSTERDADGMGLTGDFAITGEPTDM
                     HVGVAAKGVLALRLTVAGRAAHGSTPWLGENAVLRAVDMFRGVESLPFARESSELFDR
                     PSINLGRIWGGDALNKVPDSCAIDVDIRYLPHQDPAQIREQIAGLGPAAIETIIELPP
                     VSGGGTDSPWIRALTGAASIHHEGPSLSVGRDGASDAVSFIRAGIPAVEFGPVGAGHR
                     AGGMGFGPVAERVPFRPEGVPGTRSRICRKGVNR"
BASE COUNT          842 a         1726 c         1676 g         1058 t
ORIGIN      
        1 caggcatcga agtacctctc cctggtcccg aaatccatcc agtagccacc gagcggccgc
       61 gcgtagagcc ctttgccggt gatcgacggg tagaactcgg tctcgatcga gacttcccgg
      121 ccggccggta tttcggcgat tgcctcgcgt tcgagcacgt aggtgccggc gttgatcaaa
      181 ccggtgccgg ttcgctccgg atccttctcg ctgaactgga ggacctggcc gttcccgtcg
      241 atgtcgacca ggccgtagct gctcgagtcc tcgacctcgt agagacccag ggttgccctc
      301 gcgcccgttt cctcgtgctg tttccagagc gctgtgaggt cgaggtcggc gagcacgtcg
      361 ccgttgaggg cgaaaaaccg gtcgtcgagc aggtcgagcg cgtaccggat cgccccggcg
      421 gtccccaggg tgtcttcctc gaccaggtag gtgaggcggg gtcccccggg ttcaccgtca
      481 ccgaggacct ttttcatctc gtccggcaga aatccgcagg caaggatcac ttcgtcgaca
      541 ccgtggctgc ccagccactc ggtcatgtag gtgagaaacg gccggtcgac cagggtcagg
      601 gccggcttgg gggtttcata cgtcagcggt cgaagtctcg tgcctttgcc gccgaccagt
      661 accaatgcct gcaatctgtc ttacccgctt tcgtcaccgg tcccctgcct tgccgatccc
      721 ttcgtagagg aatccggcgg agcggagttt gccggcatca aggaaatttc gtccgtctat
      781 gagcaatggc ctccgcatcc gatcggccgc ctcagtccaa tcgatctcct tgaattccga
      841 ccactcggtg acgagcaccg ccgcgtctga atctgcaagg gcctcctggg ctgtcctact
      901 catttctact cccgggagga gactccttgc attactcatt gccacagggt catatgcaac
      961 aacttcggca ccctcgcctc ttagacgggc ggacaaaacc aggctcgacg cctccctcat
     1021 gtcgtccgta tccggcttga aagcgaggcc cagaagggca attcgcttgc cgaccaggga
     1081 cccgaggtgt tttgtgagct tgctcacgac ccgtcttttc tggagttcgt tgacttcgat
     1141 tacggagttc agcagctgga agtggtagcc cgagttaccg gccagtaatt tcagtgcgtt
     1201 gacgtccttg gggaagcagg acccgccaaa gccgatcccg ggacggagga aatgcggacc
     1261 gatccgctcg tcgagcccca tccctcgggc cacttccccg acgtcggccc cgacctcttc
     1321 gcagacgttg gcgatctcgt tgatgaagga gatgcgggtc gccaggtagg cgttcgacgc
     1381 gagcttgatc atctcggcac tggaggtatc ggtcatcacg aactcgcccc ccaggggccg
     1441 gtagagatcg gccacttctt cggccgcggc agaatcctcg ggttcggacc cgaccacaac
     1501 ccggtcggga tgcatgaagt cctcgaccgc ggtgccttcc ttgaggaatt cggggcagga
     1561 gacataggcc aggccgggca gttcacggcg gatcgaggcg ccggtcccgg ccggcaccgt
     1621 gcttttcatg atcagcacgt gatcaccgtt tgccttgagg tcctcaacca cggaacggac
     1681 ccgggagagg tcggcgtcgc ccgaataggt cgggggcgtg tccacgcaga cgaagagcag
     1741 ccgggccgaa tcgagaagct cgcccatgtc ggtcgtgaag gtgagccggt cccggttatt
     1801 ttcgatcagt tcggccaggc cgggctcgtg aatcgtgatc tccccgcggc tcaacgagtc
     1861 gactttctcc tggaccacgt cccgggcgat gacccggtga ccgagttcgg cgaaacaggc
     1921 tgcggttacg aggccgaccc agccagcccc gatgacgcca atcggcgcct tctcttcaga
     1981 attcatctca ctcacttgtt gggggtgaag gcccgtgtgg accgggccat gccgagcatc
     2041 tgctggttgt cgagaccgag cagcagcgaa ttcgagatcc agtacacggt gtcgccgtcg
     2101 gtccaggaga ccagcttgat ccggtcgcct tcgggataga cctggaactt gcggtcatcc
     2161 atctcgactt cgtcgtgggg tgcgtcgaga atcggcgggt cggtccatcc gcggaggccc
     2221 tgcaggccga aatagtggag gccgtcgtcg agctggactt ccatcaccat gcggtaggcg
     2281 ccatagacgt tcttgtccgg gtcacgggtg tggtagaccc ggctctggcc gtctgcgtag
     2341 atcgccccgg agggaagtcg cttggggtaa tagaccggga agtcggtacg ggtgatctgg
     2401 ctgtccaggt cctgggccac cgacaggccg ctgtctgcag catcgaccag gtcgtcgttg
     2461 ttcttgttgg ggatcttcga ggtcgaatcc tcgaggttct tcttgcgctc tctggccgcc
     2521 tgcttctctt ctcttgcctg ggccttcttc cgggccttgt tgttggcgcc gtcgccttcg
     2581 tcgagcctgc cgaccgggcc gggctggtcc tcgaagccga ggaacttgtt gaccgccgca
     2641 tcgatttcgt ccggggtggc ctcaacatag gagatgccgt cacgcatcgt gaaggtggac
     2701 gggaaggaga cttcggtaac cgcggccccg cgcgaatcga acatgagcgc aagcagctgg
     2761 atgatctggt ctccgccact gatgtccgac cgcgtgttgt cggtgaaagc gctgaggagc
     2821 tcgtcgcggc gcttccagac ttcgccgagg gtcaggcggt tgcgcacctc gccgatcaga
     2881 tcctgctgcc gggccgcccg gaccaggtcg gagtctgtgt gccggtagcg ggcaaagctg
     2941 agcgcgtccg cgccacacag tttctggtat ccggcttcga tgtcgatcgt tgcgtactgc
     3001 tcggagacgg agagtccggc gttgtcgttg aagtagtgac ggtcgacgtc cacgtacaca
     3061 cagccgatcg cgtcgatcac gtcggcaaaa ccctggaagt cgatgttgac cacatggttg
     3121 acctcgagtc cggtgattga cttgatcgtc ttcagggtca gcgccgtgcc tccgtaggtg
     3181 tatgcggcgt tgaacttgtc gtatccgtaa ccgggaatgt ccgtgtagag atcgcgggga
     3241 atcgagagca tcgcgatctg atcggtgtca gggtccagcc gcaccagcat cgccgtgtcg
     3301 gaccgtcccg gatcgccggc ggtgtcgcct cctccggtcc ggacatcgga gccgatgatg
     3361 aggatcgtct ggggcccgtc ggcgtccttg agcgcataag gcggaagcgc gatcttgttc
     3421 tcgccctcgc cgatcttggt tccgatgtcg ttcacgatgt gcatcaccga ggcctgggtg
     3481 gccgtggcga aggaaccgat gatcacgacc acggcaagca ggaagcgcca ccagtaatgg
     3541 ggacgtttgc ccggcttgtc gaagtcgccg ccggtgggcg gacgcgtcgg gtcccagaca
     3601 ttgccggtcc ggcgcgagta gtcgatcgcg tcgtggactt cggtgcgtgc cgaagcctgg
     3661 agcacggcgg tctgctcggc ggtgatcccg gtttcggacg gctgctccca ttcctgtgta
     3721 ccggtcgccg gttcctcacc ggtcaggggc tcctcgcccg tatgcgcctc ttcgccggtg
     3781 agcggttcct caccggtcgc agattcttca ccagccgaag cttcctgccc accttcgact
     3841 ttgtccccgc cctcgccggc actcgcggcg actggcgcgg cttcctggcc ggcttcgggg
     3901 gcagtctcgc cttcaggcgc agacttccct tcggcgggag ttgccgaaac ggcactctcg
     3961 ctgccttcga gcagcgcggc ccggaacacg gggtcgagat cgggtacctc gccctcctcg
     4021 acccgcgcct cgaatgcgaa aagttcctcg gtcggcggct cgacatattc gcccggcttt
     4081 tcggccggag agtcctcctg gctgccaccc gccttcgggg ccggctgcgc aggcggatac
     4141 tcgccgcctt cgggcgcgac gccttccttg tcttctggct cttcgtgctc ctcggtcacc
     4201 ggttcacccc cttccggcag atgcggctgc gcgttccagg aactccctca gggcggaacg
     4261 gtacgcgttc agcgactgga ccgaaaccca ttcctccggc ccgatgcccg gctccgaccg
     4321 gcccgaactc caccgcgggg atgccggccc tgatgaacga gacggcgtcg gatgccccgt
     4381 ccctccccac cgaaagcgaa ggcccctcgt ggtgtatcga ggccgcgccg gtcagagccc
     4441 tgatccaggg ggagtcggtc cctcccccgc tgaccggggg caattcgatg atcgtctcga
     4501 tcgcggcagg cccgagcccc gcgatctgtt cgcggatctg tgccgggtcc tggtggggga
     4561 ggtagcggat gtctacgtct atcgcgcaag agtcgggaac cttgttgagc gcgtcgccgc
     4621 cccaaatccg gccaagattt atggagggac ggtcgaagag ctcggagctt tctctcgcaa
     4681 agggtagtga ttccaccccc cggaacatgt ctaccgcccg taagacggcg ttttcgccca
     4741 gccagggagt cgatccgtgg gcagcgcggc cggccactgt taggcgcagg gccagaacgc
     4801 cctttgccgc cacgcccaca tgcatgtcgg tcggttcacc ggttatcgcg aagtctccgg
     4861 tcagccccat cccgtctgcg tcccgttccg tggactgccc gtttcgcacg agatagttgc
     4921 tgccgcgatt gtcggcctcc tccgactcct cgtcggaaac gatccccagg acaaccctca
     4981 ccccgggaac cggctcttcg gcggtaggca gcgcgagcat catcgcggcc agtgccgcct
     5041 tcatgtcata ggcaccccgg ccgatcagcc ggtcgccctc gacccgggcc tcgaactgtc
     5101 ccgcttcggc gggaaccaca tcgatgtggc cgtggaggat cacggtcgga gcatccggtt
     5161 caccccggga tgccgtgatt accggcagcc cccggcacac gccctgttcg gtttcgaccc
     5221 cgcggctgtc cagccagccc ttgatgaatc cggcagcctc atgcaccccc tcctcgtggg
     5281 aggtgtcata gccgatcaaa tg
//
LOCUS       DAOTIP010000039         5242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_18721_length_5242_cov_3.824369, whole genome shotgun sequence.
ACCESSION   DAOTIP010000039 DAOTIP010000000
VERSION     DAOTIP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18721_length_5242_cov_3.824369"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="P5138_01340"
     CDS             complement(<1..567)
                     /locus_tag="P5138_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRV59250.1"
                     /translation="MSSGIRKEGEERRPVTLTRLSEMHEAGEPIVMITAYDFPSAQVI
                     EEAGADIVLVGDSAANCVLGYDSTVPVGMDEMTMLAKAVRRGLKTPFLIGDLPFGSYE
                     GSNELAIASAQRLVKEAGCDAVKLEGGGTSVERAKAIIGAGIPVMGHVGLTPQTATAL
                     GGMKAQGRTAQQAIRVAEDAVALDQAGCF"
     gene            complement(564..1436)
                     /gene="panC"
                     /locus_tag="P5138_01345"
     CDS             complement(564..1436)
                     /gene="panC"
                     /locus_tag="P5138_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRV59251.1"
                     /translation="MKVLRTPAEVHSLRDELRNRAVTVGLVPTMGYLHEGHDSLLKAA
                     REENDVVVMSLFVNPTQFRAGEDLSTYPRDEERDLAIAGQSGVDYVYAPLPDDIYPSG
                     YATRVEVEGLTEVLCGSETSRGPGHFAGVTTIVAKLLNAVDPDVAYFGQKDAQQATVI
                     RRMAEDLEFRTRIDILPTVRERDGLAMSSRNAYLSPEARERATAIWASLLETETVFAE
                     SGLDAALDAGRHHLESAGITPEYFEARYPGTLDPADGEGSDPILVAVAAKVDGTRLID
                     NILIEPERDNRRKP"
     gene            complement(1433..2209)
                     /locus_tag="P5138_01350"
     CDS             complement(1433..2209)
                     /locus_tag="P5138_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRV59252.1"
                     /translation="MKASEAALRAPVIVGPGRVGRSLAAALGGEGGAVDLRDRTSSLD
                     DLAGRVVLLSVPDAAVSEVAGRIGASGEAPSLIGHTSGATTLDALRGAAADGCFSIHP
                     LQTVPDGSTDLAGCPAAVAGSGPEALDAAVRLAEVAGMAPFEVSETDRATYHAAASIA
                     SNFLVTIEETAAELLGGIAVENPREVLEPLVRRSLDNWLERGPQALTGPIARGDEATV
                     AAHRDALLERRSELVPFYDALADRTRQIAGNGRSAEGAPR"
     gene            2333..2794
                     /gene="tsaE"
                     /locus_tag="P5138_01355"
     CDS             2333..2794
                     /gene="tsaE"
                     /locus_tag="P5138_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRV59253.1"
                     /translation="MSGVAEEPRGGFLAHGQGETEAAGRDLAALIEPGDLILVNGEVG
                     AGKSTLVRAALRGLGVEGPIPSPTFTIGRLYDQPAQRMPVSHLDLYRLGDLAAEEPGL
                     LSEYLGPDRAAFVEWPGGSDVQLREMSTRIGRVDIDHIDSESRRIRIEPPA"
     gene            complement(2801..3163)
                     /locus_tag="P5138_01360"
     CDS             complement(2801..3163)
                     /locus_tag="P5138_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59254.1"
                     /translation="MKMIENRKLGTGLAILAVAGLGVFATGCGDSSSDKVNDAIDNAN
                     QQLDKANQQLDQANQNAQDALDKANKQLDQASQSAQDQIDQAQKKLNEQNAQQKVDDA
                     QKQLDQIQQQVQDAMNGQ"
     gene            3227..3937
                     /gene="tsaB"
                     /locus_tag="P5138_01365"
     CDS             3227..3937
                     /gene="tsaB"
                     /locus_tag="P5138_01365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRV59255.1"
                     /translation="MRVLAFDTATDDTVVASIDGDRVAFERTVPPEGRPLHSQALLSL
                     ASEAADALGGWDGVERIAVGIGPGTFTGIRIGVATAAGLAASTGIPVTGISTLAALAR
                     SIGEAAGTGPVMPLLDARRGEVFGGLHDAAGAPMGAPFVCGPEELMRQLGSREGVVPL
                     VAGPGAVRFRDELLRAGFETEPDGSSVHRLAGRPVCELGAMAEKTGPATLEPLYLRIP
                     DAQLWLERDGNSPDANRN"
     gene            3897..4442
                     /gene="rimI"
                     /locus_tag="P5138_01370"
     CDS             3897..4442
                     /gene="rimI"
                     /locus_tag="P5138_01370"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRV59256.1"
                     /translation="MNETATAPTRTETDRQGPVGGIRVGALAYADLPLVISIERRSFR
                     SPWSVGMFALEMSKQDTIGLTAVEDDRVIGYLILSRFDRAWHLMNVAVAPEARRRGIA
                     SLLIEEAIGMIEPRDPVTLEVRPTNQSAVRLYEGLGFRAFGLRPGYYPDNGEDALIMW
                     RGDPEAAGVPRESFGEIPAGT"
     gene            4442..>5242
                     /gene="tsaD"
                     /locus_tag="P5138_01375"
     CDS             4442..>5242
                     /gene="tsaD"
                     /locus_tag="P5138_01375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRV59257.1"
                     /translation="MPLLAIETSCDDTCAAVLDGAAVLSNVISSQVDHARYGGVVPEV
                     ASRHHLELAIPVLDAALAEAGTGLGEIDSVAVTAGPGLIGALLVGLSTAKAIAAPREL
                     PLIPTDHLHGHVAANFLEPDPLEPPFLCLIASGGHTLLAGVTDHRGFEVLGETLDDAA
                     GEAIDKAARMLGLGFPGGPELERLAATGDPGAFSFPVAMSRDPGLDFSFSGLKTALVY
                     ICRDLGESETDDRRADLAAGFQAAVVDQLVTKLGRALDRGDWPAVALGG"
BASE COUNT          798 a         1712 c         1769 g          963 t
ORIGIN      
        1 gaagcaaccg gcctggtcga gcgcgactgc gtcctccgcc acccggatcg cctgctgggc
       61 cgtccgtccc tgggccttca tgccgccgag agcggtggct gtctgcgggg tgagcccgac
      121 atggcccatt acggggatgc cggcgccgat gatcgccttg gccctttcga cagacgtgcc
      181 gccgccttca agcttgacgg cgtcgcatcc ggcttccttg accagccgct gggcgctcgc
      241 gatggccagc tcgttcgagc cctcgtatga cccgaagggc aggtctccga tcaggaaggg
      301 tgtcttgagg ccgcggcgca cggccttggc gagcatggtc atttcgtcca tgcccaccgg
      361 cacggtcgag tcgtacccga gaacgcagtt cgcagccgaa tcgccgacca gcacaatgtc
      421 ggcgccggcc tcctcgatca cctgggccga cggaaagtcg taggcggtga tcatcacgat
      481 gggttccccg gcctcatgca tctcgctcag ccgggtcagt gtcacggggc ggcgctcctc
      541 gccttccttg cggattccgg agctcatggc ttcctcctgt tgtctcgttc aggttcgatc
      601 aggatgttgt cgatcaggcg tgtgccgtcg accttggcgg cgacggcgac gaggatcggg
      661 tcggacccct ccccgtcagc cggatccagg gtgcccggat agcgggcttc gaagtattcg
      721 ggggtgatgc cggccgactc gagatggtga cggccggcat cgagcgctgc gtcaaggccg
      781 gactcggcaa agaccgtttc ggtctccagc agcgacgccc agatcgcggt cgctctctca
      841 cgggcctcgg gggagaggta ggcattgcgc gaactcatgg ccagcccgtc ccgctcacgg
      901 acggtcggaa ggatgtcgat cctggtccgg aactcgagat cctcggccat gcggcggatc
      961 accgtggcct gctgggcatc cttctggccg aagtaggcga cgtcggggtc gaccgcgttg
     1021 agcagtttgg cgacgatcgt ggtcactccg gcgaagtgac ccgggccgcg gctggtttca
     1081 cttccgcaaa gcacctcggt cagtccctcg acctcgaccc tggttgcgta gccggaggga
     1141 tagatgtcgt cgggcagggg cgcgtacaca tagtccacac ccgactgccc ggcgatcgcc
     1201 aggtcccgtt cctcatcccg ggggtaggtg gagaggtctt cgccggcccg gaactgggtc
     1261 gggttcacga agaggctcat cacgaccacg tcgttctctt cccgggcggc tttcagcagc
     1321 gagtcgtggc cttcatgcag atatcccatg gtcgggacca ggccgaccgt gacggcccga
     1381 tttcgcagct cgtcccgcag cgaatgcacc tccgccggag ttcgcagaac cttcatcggg
     1441 gtgctccctc ggccgacctg ccgttaccgg cgatctgccg ggttctgtct gcaagggcgt
     1501 cgtagaacgg aaccagttcg gaccggcgct cgaggagcgc gtcgcgatgg gccgcgacgg
     1561 tggcttcgtc gccgcgcgcg atcggtccgg tgagcgcctg cggtcctcgt tcaagccagt
     1621 tgtccagact gcgccggacc agcggctcga ggacttcacg ggggttctcg accgctatcc
     1681 cgccgagcag ttccgccgcc gtctcctcga tcgtcaccag gaagttggaa gcgatgctgg
     1741 ccgccgcgtg gtaagtcgcc cggtcggttt cgctcacttc gaatggcgcc atgcctgcaa
     1801 cctcggccaa acggacggcg gcgtcaaggg cctcggggcc ggagccggca acggcggccg
     1861 ggcagccggc cagatctgtg ctcccgtcgg gaacggtctg caggggatgg atggaaaagc
     1921 agccgtctgc ggcagcaccg cgcagagcgt ccagggtggt cgcaccgctg gtgtggccga
     1981 tcagggacgg ggcttcccct gacgcgccga tccggcctgc gacttccgag accgctgcgt
     2041 cgggaacgct cagcaacacc acgcgtccgg ccaggtcgtc gagagacgaa gtccggtccc
     2101 gaagatccac cgctccgccc tcgccgccaa gggcggcagc cagcgaacgg ccaacccggc
     2161 ccgggccgac gattacgggc gcgcgaagcg ctgcttctga agctttcatt ttgccgttcc
     2221 agttcccttt cggcgatacc agacggatcg agtttggttt tcctgcggtg aggggtttcg
     2281 gtccaccccc ggaatcccgg gtggcagcca caggcaatta taggctgccg ccatgtcggg
     2341 tgttgccgaa gagcctcgcg gagggttcct tgcccatggc cagggggaga ccgaggctgc
     2401 gggaagggat ctggcggccc tgatcgaacc gggcgacctg attctcgtca acggcgaagt
     2461 tggcgccgga aaatcgaccc tggtcagggc tgcgctacga gggctcggcg ttgagggtcc
     2521 gatcccttcg ccgaccttca cgatcggaag gctctatgac cagccggccc agaggatgcc
     2581 tgtttcgcac ctggacctct accgtctggg agacctggcc gccgaggaac cggggctcct
     2641 ttccgaatat ctgggccccg accgggctgc attcgtcgaa tggccgggtg gttccgatgt
     2701 ccagctgcgt gaaatgtcca cccggatcgg ccgggtggac atcgatcaca tcgattcgga
     2761 gagtcggcgg atcaggatcg agccgcccgc ctgaagcggc ctactggccg ttcatcgcgt
     2821 cctggacctg ctgctggatc tggtcgagct gcttctgggc gtcgtcaacc ttctgctggg
     2881 cgttctgctc gttgagcttc ttctgggcct ggtcgatctg gtcctgcgcg ctctggctgg
     2941 cctggtcgag ctgcttgttg gccttgtcaa gggcgtcctg cgcgttctgg ttggcctggt
     3001 cgagctgctg attggccttg tcaagctgct ggttggcgtt gtcgatggcg tcattgacct
     3061 tgtccgagga cgaatcacca cagccagttg caaaaacgcc aagtcccgct actgcgagaa
     3121 ttgccaaacc cgtgccgagc ttcctgtttt cgatcatctt caagtctcca gtttcgtttg
     3181 ccgcggtgga ctccctcgtg acaccgccag attaccggtg tccgaggtga gagtacttgc
     3241 attcgacacc gcgactgacg acacagtggt cgcttccatc gacggggaca gggtcgcttt
     3301 cgagcggacg gttccgccgg aaggccggcc tcttcacagc caggcgctgc tatctctggc
     3361 gagcgaggca gcggacgcgc tcggaggatg ggacggtgtg gagaggatcg ccgtggggat
     3421 cggccccgga accttcacgg gaataaggat cggggtggcc actgcggccg gtcttgccgc
     3481 ctccacggga atcccggtga ccggcatatc gactctcgcg gccctggcca gatcgatcgg
     3541 tgaggcggcc gggaccggcc cggtcatgcc gctgctcgat gcccggcgcg gagaggtgtt
     3601 cggtggtctc cacgacgcgg ccggggcgcc gatgggtgcc ccctttgtct gcgggcccga
     3661 agaactgatg cggcaactcg ggtcgcggga aggcgtagtt ccgctggtcg ccggaccggg
     3721 ggcggtacga ttccgagacg aattgctccg agccgggttt gaaacagagc cggacggatc
     3781 atcggtccat cggctggccg gccgcccggt ttgtgaactt ggcgccatgg cagaaaagac
     3841 aggaccagca accctcgaac ccctctacct gcgaatcccc gatgcacaac tctggcttga
     3901 acgagacggc aacagccccg acgcgaaccg aaactgaccg gcagggcccg gttgggggga
     3961 ttcgggtagg tgcgctcgcc tacgcggacc tgcccctcgt catttcgatc gaacgtcgct
     4021 cgttccgcag cccgtggtcg gtcggcatgt tcgcgctgga gatgtcgaag caggacacaa
     4081 tcggactgac cgccgttgaa gacgaccggg tgatcggcta cctgatcctc tcccgtttcg
     4141 accgcgcctg gcacctgatg aacgtcgccg tcgcccccga ggccaggcgc cgcgggatcg
     4201 cgtcgctgct gatcgaagag gcaatcggga tgatcgaacc ccgcgacccc gtgacgctcg
     4261 aggttcgacc gaccaaccag tccgcggtcc ggctgtatga aggactcggc ttccgggcct
     4321 tcggcctgcg gcccgggtat tacccggaca acggcgagga tgcgctgatc atgtggcgcg
     4381 gtgatccgga ggcggccgga gtccccaggg agtcgttcgg ggaaatccct gccggtacct
     4441 gatgccgctg ctggcgatcg agacctcctg cgatgacacc tgtgctgcgg ttctcgacgg
     4501 agccgcggtt ctctcgaacg tgatttcctc gcaggttgac cacgcgcgct atggcggcgt
     4561 ggttccggag gtggcgtcgc ggcatcatct cgaactggcg attccggttc tcgatgcggc
     4621 ccttgccgag gccggaaccg gtctgggcga gatcgactcg gtcgccgtca ccgccggccc
     4681 cggtctgatc ggagccctgc tcgtcggact gagcacggcc aaggcgatcg ctgcgccgag
     4741 agaactgccc ctgatcccga ccgaccatct tcacggtcac gtcgccgcca atttcctcga
     4801 gcccgacccg cttgagcccc ctttcctgtg tctgattgct tctggcgggc acacgctgct
     4861 ggccggggtc accgaccaca ggggtttcga ggtcctgggc gaaacccttg atgatgcggc
     4921 gggcgaggcc atcgacaagg ctgcccgcat gctcggcctc ggattcccgg gcggcccgga
     4981 actcgagcga ctggccgcaa cgggggaccc cggtgccttt tcgttcccgg tcgccatgag
     5041 ccgggacccc ggcctcgatt tcagcttcag tggcctcaag accgcactcg tctacatctg
     5101 ccgggatctg ggcgagtccg agacagacga tcgccgggcc gacctcgccg ccggtttcca
     5161 ggccgcggtg gtggaccagc tggtcaccaa gctcggaagg gcgctcgacc ggggggactg
     5221 gccggcggtc gcactcggcg gg
//
LOCUS       DAOTIP010000040         5219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_18813_length_5219_cov_3.106507, whole genome shotgun sequence.
ACCESSION   DAOTIP010000040 DAOTIP010000000
VERSION     DAOTIP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18813_length_5219_cov_3.106507"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="P5138_01380"
     CDS             <1..1519
                     /locus_tag="P5138_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRV59258.1"
                     /translation="ACLAGIAGTASGAGLKRITPEGYFGSDPVFLTSPPGDSRQFVVE
                     RGDESTHQARVRIIENGQPLSEPFLTVDNVNLEGERGLLSMAFAPDYATSGLFYLFYV
                     VNERGDPFGGTEGDIRIVEYHVSDSDPDKADPNSARLVFRTPHYTGYHQGSWMAFGPD
                     GYLYFSIGDDGYGVNAQQLDTFFGKIFRIDPADPDGAGSATYSVPADNPFVGVAGAKP
                     EIYAYGLRNPFRASFAPDGRLVVGEVGNADWEEIDVGDLKGANLGWPVCEGFCTGSHP
                     EFTEPIYTYAHGPNNCAVIGGYVVQGTGMGALSGRYLFGDLCDLALRTLDLDAAGGDP
                     RPAGFSIPEDGGYLRSFGEDSSGCVYVLTSLAVFKVVGESATGSTCPGPPDPTPAATY
                     SSSFPRREVIAARLTVRARCSIACSASANAKVRISRNRHHRRPVTYNLKTAAAELAAG
                     VQGRLTFKVPAKRVRSMKLALRNGSSVAARVRIRMAGSDGSGKTRYGRIRLVRPR"
     gene            1536..3107
                     /locus_tag="P5138_01385"
     CDS             1536..3107
                     /locus_tag="P5138_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRV59259.1"
                     /translation="MKWITAAGVVMLCWLGISGSAFGAGLVRVTPDGYLGSDPSFLTS
                     PPDDSRVFIAERGDSGSHQAQVRIIKDGVPVNQPFLTIGNVDLVSERGLLSIAFAPDY
                     ATSGLFYAFYVAHAADGFGGSTGDIRIVEYHVSSGDPDKADPASARLVFSVPHSAGNH
                     NGGWMAFGPDGDLYFSIGDNANGSNAQTLTNVYGKIMRIDPADPDGAGAATYTVPSDN
                     PFVGEAGARTEIYALGLRNPYRASFTPDGRLVIGDVGNGTWEEVDVGDLKGANLGWPD
                     CEGFCTTLTPSFAAPIYVYNHDNGVDNQGGGCAIIGGYVVRDESLGGLTGRYLYGDLC
                     DDALRTLNLDAAGADPQQAGLVIPDGVGSLRSFGEDSGGCVYVLGNSAVYRVAPNGST
                     ATACPVTTEPPGQVTYNSFIPQRRVIAKRLVVGAKCSIACTASATTKVRITRNRFRRK
                     PAIFNLKTPVSDLAAGVRGKLTFQVPLKRVKAMKKAVRNGSRVTARVKVTLTGEDGSG
                     GSGSGSIRLVRPKRR"
     gene            complement(3115..3597)
                     /locus_tag="P5138_01390"
     CDS             complement(3115..3597)
                     /locus_tag="P5138_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRV59260.1"
                     /translation="MGKKKKAGRVSAGLILYRSGPAGLEVLIAHMGGPLWAKKERGWS
                     IPKGEIDPGEEPLETARREFREEVGMDPPAGVPIHLGEITQKSGKRVLAWALEGDLNP
                     GDQVSVTCEIEWPPRSGERIEIPEVDRVEWVAPSTAKNLVIEGQMPFFDRLEKLVASG
                     "
     gene            complement(3647..4639)
                     /locus_tag="P5138_01395"
     CDS             complement(3647..4639)
                     /locus_tag="P5138_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59261.1"
                     /translation="MSGRISPTEARFARADIGAGMYESWYARLVSPDQPLAVWIRYTI
                     DKLPGREATGTLWFTLFEGERDRPLARRQTGQPADVPAGEWVRVGESTIGGGALNGRC
                     FEAGWDLTWEPRAPVLFHLPRAKLYEFPVPKTNPISPVPDGDFNGEIRVGDRRIRVDG
                     WRGMIGHNWGSEHAERWIWLHGSGFEEDPDAWIDIAMGRLKIAGRLLPWVCNGAVHIN
                     RETRRVGGLFATGVKVDETPTRLEASVPVSGRRKLNLEVESPPNLTVGWRYGDPADPE
                     AHAHEVANCSAASMTARLSGGGSETVLRTDHGAAYELGMAETDHGVPISEGLPS"
     gene            complement(4653..5003)
                     /locus_tag="P5138_01400"
     CDS             complement(4653..5003)
                     /locus_tag="P5138_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRV59262.1"
                     /translation="MKFMLLIHQGTTPLPGTPEWDALSGEEQGEVYAGYQAVNETPGM
                     TPGERMADPADAKTVRVEDGGPILVDGPFAEIKEALGDLLFLEADDLQAAVDLAAKIP
                     AARMGGAVEIRPLL"
BASE COUNT          863 a         1799 c         1670 g          887 t
ORIGIN      
        1 ggcatgcctg gccgggattg ccgggaccgc ttccggggcc ggcctcaaac ggatcacccc
       61 ggagggctac ttcgggtcgg atccggtctt cctcacctcg ccgccgggag attcgaggca
      121 gttcgtcgtc gagaggggtg acgagtcaac ccatcaggcc agggtccgca tcatcgaaaa
      181 cggccagccg ctcagcgaac cgtttctgac cgtcgacaac gtaaacctgg aaggcgaacg
      241 cgggctgctc tcgatggcct tcgccccgga ctacgcgacc agcggcctgt tctacctgtt
      301 ctatgtcgta aacgaacggg gcgacccgtt tggcggaacc gagggagaca tccggatcgt
      361 cgaataccac gtctccgaca gcgatcctga caaggccgac cccaactccg cgcggctcgt
      421 cttcaggact ccccactaca ccggctacca ccagggcagc tggatggcct tcggtcccga
      481 cggatacctc tacttctcga tcggggacga tggatacggg gtcaacgccc aacaacttga
      541 caccttcttc ggcaagatct tccggataga tccggccgac cccgacggtg ccgggtccgc
      601 cacctactcg gtgccggccg acaatccctt tgtcggcgtg gccggcgcca agccagagat
      661 ctatgcctac gggctccgca acccgttccg ggcttcgttc gcgccggacg gaaggctcgt
      721 ggtcggtgag gtcggcaacg cagactggga agagatcgac gtcggcgacc tcaagggggc
      781 gaacctcggc tggccggtct gcgaggggtt ctgcacggga tcgcacccgg agttcaccga
      841 accgatctac acctacgcgc acggaccgaa caactgcgcg gtgatcggcg gctacgtggt
      901 ccaggggacc gggatgggcg ctctttcggg ccggtacctg ttcggagacc tctgcgacct
      961 tgccctaagg accctggatc tggacgccgc cggaggcgac ccgcgtccgg ccggattcag
     1021 catcccggag gatgggggct accttcgctc cttcggcgag gactcaagcg gctgcgtcta
     1081 cgttctgacc agcctggccg tcttcaaggt ggtcggcgaa agcgcgaccg ggtccacctg
     1141 cccggggccg cccgacccga ccccggcggc aacctacagt tcgtcttttc cccgccggga
     1201 agtgattgct gcccgcctga cagtcagggc cagatgctcg atcgcctgct ccgcctcggc
     1261 gaacgcaaag gtccgaatca gtcgcaaccg ccaccacaga aggccggtga cctacaacct
     1321 gaagactgcg gccgctgaac tggcggccgg cgttcagggc cgattgacct tcaaggtgcc
     1381 agcgaaacgg gtccggtcga tgaagctggc cctgcgaaac ggcagcagcg tggccgcccg
     1441 ggtaaggatc aggatggccg gatccgatgg atccgggaag acccggtacg ggcgcatccg
     1501 tctggtccgt ccccgatgac ggtagtttga tccccatgaa gtggatcact gcggcggggg
     1561 tcgtgatgct ttgctggctc ggaatcagcg ggtcggcgtt cggcgcgggg ctggtccggg
     1621 tcactccgga cggctacctc gggtccgacc cgagcttcct cacctctccc cctgacgact
     1681 cccgggtctt catcgccgaa cggggtgact cgggcagcca tcaggcccag gtccggatca
     1741 tcaaggacgg cgttcccgtg aaccagccct tcctgaccat aggcaacgtc gacctggtca
     1801 gcgagcgggg gctgctctcg atcgcctttg ctcccgacta cgcgaccagc ggactcttct
     1861 acgccttcta cgtggcccac gcggccgacg gcttcggcgg cagcaccggg gacatcagga
     1921 tcgtcgagta ccacgtgtca tcgggggatc ccgacaaagc cgaccccgcc tcggcccggc
     1981 tggtcttttc ggtcccacat tcggcgggga accacaacgg cggctggatg gccttcggcc
     2041 ccgacggtga cctctacttc tcgatcgggg acaacgccaa cggctccaac gcccagacgc
     2101 tcaccaacgt ctacggcaag atcatgcgga tcgacccggc cgaccccgac ggagccggcg
     2161 ctgccaccta caccgtgccg tccgacaatc cgttcgttgg cgaggccgga gcgagaaccg
     2221 agatctacgc gctgggcctg cgcaacccct accgggcctc cttcacccct gacggcaggc
     2281 tcgtgatcgg tgacgtcggc aacggcacct gggaggaggt cgacgtcgga gacctcaagg
     2341 gcgccaatct cggctggccg gactgcgagg gattctgcac cactctgacc ccgtcctttg
     2401 cagctccgat ctacgtctac aaccacgaca acggtgtcga caatcagggc ggcggctgcg
     2461 cgatcatcgg tggctatgtg gtccgggacg agagcctcgg cggcctgacc ggccgctatc
     2521 tctacggcga cctctgcgac gacgcgctgc ggaccctgaa cctcgacgcg gcgggcgcgg
     2581 acccgcagca ggccggactc gtgattcccg acggtgtcgg ctcgctgcgc tccttcggcg
     2641 aggactcggg aggctgcgtc tacgtgctcg gcaactctgc cgtgtaccgg gtcgcgccga
     2701 acgggtcaac tgccaccgcc tgcccggtca cgaccgaacc gccggggcag gtcacctaca
     2761 actcgttcat cccgcaacgg agggtgatcg ccaaacgcct ggtggtcggg gcgaagtgct
     2821 cgatcgcctg caccgcttcg gccacgacga aggtgcggat caccagaaac cgcttccgca
     2881 ggaagccggc gatcttcaac ctgaagaccc cggtctccga ccttgcggcc ggggttcgag
     2941 gcaagctcac cttccaggtg ccgctcaagc gggtcaaggc catgaagaag gccgttcgca
     3001 acggcagccg ggtcaccgcc cgggtcaagg tgaccctgac cggcgaagac ggttccggcg
     3061 ggtcgggttc cggttcgatc cggctggtcc ggcccaaacg gcgctaaccg gtcctcagcc
     3121 ggaggcgacc agcttctcga gccggtcgaa gaacggcatc tgtccctcga tcaccaggtt
     3181 cttcgcggtc gagggcgcga cccactccac ccggtcgacc tccggtatct cgatccgctc
     3241 acccgagcgt ggcggccact cgatctcaca ggtcacgctc acctggtcgc cggggttcag
     3301 gtccccttcg agcgcccagg cgaggacccg cttgcctgac ttctgggtga tctccccgag
     3361 gtggatcggt acgccggccg gcgggtccat gccgacctct tccctgaact cgcggcgggc
     3421 cgtttcgagg ggctcctcgc cgggatcgat ctcgcccttg gggatcgacc agccccgctc
     3481 cttcttcgcc cagaggggac cgcccatgtg ggcgatcagc acctcgaggc cagccggacc
     3541 gctgcggtag aggatcagtc cggcactgac ccggccggct ttcttcttct tgcccatccc
     3601 ggcgaggcta gctgctcggg tgaatcatct gccgtgccgg gcagctctat gacggaaggc
     3661 cttcagagat cggaactccg tggtcggtct cggccatgcc gagctcgtaa gcggctccat
     3721 gatcagtgcg aagaaccgtt tcgctcccgc cgccggagag cctcgcggtc atgctggccg
     3781 ccgagcagtt cgccacctcg tgagcgtggg cttccgggtc ggccgggtcg ccgtaacgcc
     3841 agccgacggt cagattcggc ggcgattcga cctccaggtt cagcttccgg cgcccggaaa
     3901 cagggacgga cgcctcaaga cgggtggggg tctcgtcgac cttgaccccg gtcgcaaaaa
     3961 ggccgccgac ccggcgggtt tcgcggttga tgtgaacggc tccgttgcag acccagggga
     4021 gcaggcgccc ggcgatcttc agccggccca tggcgatgtc gatccaggcg tccggatctt
     4081 cttcgaaccc gctgccgtgg agccagatcc atcgctcggc atgttcgctc ccccagttgt
     4141 gaccgatcat gccgcgccat ccatcgaccc ggatcctgcg gtcaccgacc ctgatctccc
     4201 cgttgaaatc cccgtccggc acgggtgaga tggggttggt cttcggaacc ggaaactcgt
     4261 agagctttgc ccgcggcagg tggaagagga cgggtgcacg cggctcccag gtgaggtccc
     4321 agccggcttc aaagcaccgg ccgttgagag cgccgccacc gatcgtcgac tcgccgaccc
     4381 tcacccactc cccggccgga acatcggccg gctggcccgt ctgacgacgg gccaggggcc
     4441 ggtcccgttc accctcgaag agggtgaacc agagagttcc ggtcgcctcg cggccgggga
     4501 gcttgtcgat cgtgtaccga atccagacgg ccaaaggctg atctggcgaa acgagccggg
     4561 cgtaccagga ctcgtacatg ccggccccga tgtccgccct ggcgaacctc gcttcggtcg
     4621 gtgagatcct gccgctcaca ggggaaggag tatcagagca gcggcctgat ctccaccgcc
     4681 ccgcccatgc gggcggccgg gatctttgcg gccaggtcca ctgccgcctg gagatcgtcc
     4741 gcctcgagga agaggaggtc gccgagcgcc tccttgatct cggcgaaagg accgtcgacc
     4801 aggatcggcc cgccatcctc gacccggacc gtcttcgcgt cggccggatc agccatccgc
     4861 tcgcccgggg tcatacccgg ggtctcgttg actgcctggt atcccgcata gacctcgccc
     4921 tgttcctcgc cggaaagggc atcccattcg ggagttcctg gcaagggcgt ggtgccttgg
     4981 tggattagca gcatgaactt catttgttcc tccttggtgg atgttcgcct atttgacgaa
     5041 cgcccacggg aagaatcgac atttcagcga aaagaaatcc gccgggtcga atgggcggtg
     5101 ttgccgagcc ggtcccagga ctttatccgg agccgcgcac ggcgctgccc ggaaaggttc
     5161 cggttcagct tcacggccac ccgcttcgtc ttgccggcct tgatcctgaa acgtgcctg
//
LOCUS       DAOTIP010000041         5144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_19182_length_5144_cov_3.128709, whole genome shotgun sequence.
ACCESSION   DAOTIP010000041 DAOTIP010000000
VERSION     DAOTIP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19182_length_5144_cov_3.128709"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="P5138_01405"
     CDS             <1..499
                     /locus_tag="P5138_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59263.1"
                     /translation="LACSDDLDAQRADARTLVTLLHLREINERLDPGFSIVTEMVDER
                     SRRLAEVAEVDDVIVSENVISLLFSQISQNHHLAEVFTQLFSPEGSEIYLRPASTYVA
                     VNGRVNFATLVEAARRRGECAIGYRTSAEAKNPDSAFGMTMNPPKSAQFRISPGDSLV
                     VLAED"
     gene            complement(639..1925)
                     /locus_tag="P5138_01410"
     CDS             complement(639..1925)
                     /locus_tag="P5138_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRV59264.1"
                     /translation="MSVAQAEPRGNSAGSISYMDLYRRWEENNWSAMSLDFSADRAGW
                     DSLSELQRESALWMYSMFFYGEDSVADNLSPYVDAAPTEEQAYFLTTQQVDEARHAVL
                     FHRFFREVIGIDGDVGTTLEATLPRLNWGYRGVFDRLDRMADELRADRSLPKFAQAIT
                     LYHLIVEGTLAQPGQHFIEDYFNKDGGMPGFSAGMANVSRDEQRHIGFGVKTLSEIVP
                     QSEECKAAIVELFREVNLHSVGVFCPPGFDRSFTECWGFTMEDIYAFGLKLVRQRWKT
                     IGFPLEEMPADVWPFDHSLTAEEIAAQQVRLLLAGVTGAPNAKPDSSPEIQGLLFDMT
                     ARRADHAKAGHGPFRVQWDFVDAEPWNVIVNNGSTRAEQGRIPDPDVTLKTTWPEWVG
                     IALNERNPLRAILERRLTPKGSPRALATFRKVFPPL"
     gene            1998..2669
                     /locus_tag="P5138_01415"
     CDS             1998..2669
                     /locus_tag="P5138_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV59265.1"
                     /translation="MNESGAGQSEDQPAVRRRMPAAERRKLILDAALDAFAEGGYEET
                     TLDEVAERAGVSKALIYEHFPSKRDLYREILETWVGELLGRVLAAATGAGERSEDRLV
                     AGLDGFLIFVEERRDAWRLLIRHRASDDVSETFDRLFEEVARIIGALMAPEMPKSVTP
                     EGVDFDLVLDATSRQLMGAVTAIANWWDEHREVPRDRVLAMVMEFAWVGMERAAAGES
                     WSGGH"
     gene            complement(2663..4357)
                     /locus_tag="P5138_01420"
     CDS             complement(2663..4357)
                     /locus_tag="P5138_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRV59266.1"
                     /translation="MKLFPSIRPYSRGWLRRDLLAALTVWAVLVPEALAYATIAGVSP
                     IVGLYAAPGALLIYAALGSSRRLVTGADAATAALSAAIVGGIVTGDSSAFVQTTAALA
                     ICVGVIAFAAGIARLGFVVNFISIPVLRGFIVGLALTIIAGQIPYLFGVESSNGNFFN
                     RIWVFLTHLGQVDLPTVAIGLISLAIILFCKYRRPAVPGSLIAVVLGVAAVNAFDLGA
                     HGVEIVGQVNSDLPSISLPDIGLADLGPLVAGAVGLMLVGFAESLASAKAYPDSEESL
                     DPNRELVGTGAANVGAGLFGGFVVTGSFSKTSINADSGARSQLSGIVVAVLAILTMLF
                     LTGLFEDLPEAALAAVVIAALIHAVDIVGLRDLARIRERGNPLNPANRPDFIASIAAL
                     AGVMVFDILPGLFIGIGVSLVLLLYRSSRPRVAELGELKSQPTHWGDRRRHPDALEVP
                     GTVVLRLEAAIFFANAEEIRKEVIGSIRPDTRAVVLDVETVPYIDVTGVYMLRSLYEE
                     LRRRQIRLAVARDVGQVEDMMRATGLAGLLADSYVSIDQAVDTVSAGDGSPHDPGQ"
     gene            complement(4354..>5144)
                     /locus_tag="P5138_01425"
     CDS             complement(4354..>5144)
                     /locus_tag="P5138_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59267.1"
                     /translation="AVSAAVLAGGGVLLVNQAAISAVLVATLPAPDLVDSTHRFIDAL
                     VGGGVALTANAITPVDPVRLVRRYLEPLLSRLSGTLVDIADALDHNSHEEIVAALERA
                     RGLDPAVREMKIAVEAGQETISLAPTRRRFRGRVDQLAAAAEPIDLMIRNTRVLARGC
                     QRAIDLDENIPPVVSDSIRDLATAVARLDQHLGGAPARSATRESALRAAAKATAALEE
                     TSNLSVSVIVGQIRSAATDLLLSLGMSSEDALKEVRSAQERLGL"
BASE COUNT          943 a         1630 c         1734 g          837 t
ORIGIN      
        1 actcgcctgt tcggatgatc tcgacgccca gcgtgcggat gcccggacgc tggtgaccct
       61 gctccacctg agggagatca acgaacggct ggatccgggc ttttcgatcg tcaccgaaat
      121 ggtcgacgag cggagccgcc ggcttgccga agtcgccgag gtggacgacg taatcgtcag
      181 cgagaacgtg atcagcctgc tcttctccca gatatcgcag aaccatcacc tcgccgaagt
      241 gttcacccag ctcttctccc cggaagggag cgagatctat ctcagaccgg cctccaccta
      301 tgtcgcggtg aacggacggg tgaacttcgc caccctggtc gaggcggccc gtcgccgcgg
      361 cgaatgcgcg atcggatacc ggacctcggc agaagcgaag aatcctgatt cggccttcgg
      421 tatgaccatg aatccgccga agtcggcaca gttcaggatc agtccgggtg acagcctggt
      481 ggttcttgcc gaggattgac ctgaccggtc aaatgcaagg cggcgagacc gaggcctcac
      541 cgcctcccct gcttctgtca gtccctgccg tgcatccctc cctggcacac gcggactgcc
      601 cctataaccg ggctggcggc cgagggttgc ggtgcaactc agagtggcgg aaaaactttc
      661 ctgaaggtgg cgagggcacg cggtgagccc ttgggtgtga gacgccgctc gaggatggcc
      721 cggagcgggt tccgctcatt cagcgcaatg cccacccatt cgggccaggt cgtcttcagg
      781 gtcacgtcgg gatcgggaat acggccctgc tcggcccggg tcgaaccgtt gttcacgatc
      841 acgttccagg gctcagcgtc gacgaaatcc cactggaccc tgaacgggcc gtgaccggcc
      901 ttcgcgtggt ctgcccggcg ggcagtcatg tcgaacagca gtccctggat ctccggtgag
      961 gaatcgggtt tggcgttcgg cgcgccagtc actccggcca gcaacagtct cacctgctgt
     1021 gcagcgatct cctcggcggt cagcgaatga tcgaacggcc agacatcggc cggcatctcc
     1081 tccagcggaa atccgatcgt cttccagcgt tgccgcacca gcttgaggcc gaaggcgtag
     1141 atgtcctcca tcgtgaatcc ccagcactcg gtgaacgagc ggtcgaagcc gggcgggcag
     1201 aagactccga ccgaatggag gttcacctcc cggaagagtt ctacgatcgc cgccttgcat
     1261 tcttcggact gggggactat ctcggaaagg gtcttcactc cgaagccgat gtggcgttgc
     1321 tcgtcgcggg aaacattcgc catgccggcg ctgaaacccg gcatgccgcc gtccttgttg
     1381 aagtagtcct cgatgaagtg ctgccccggc tgggccaggg ttccctcgac gatcaggtgg
     1441 tagagggtga tcgcctgggc gaacttcggc agggagcggt ccgcccggag ttcgtcggcc
     1501 atacggtcga gccggtcgaa aaccccgcgg tagccccagt tgagccgggg cagggtggcc
     1561 tcgagcgtgg tgccgacgtc accgtcgatc ccgatcacct ccctgaaaaa gcggtggaag
     1621 aggacagcgt ggcgagcctc gtccacctgc tgggtggtca ggaagtaggc ctgctcctcg
     1681 gtcggcgcag catcgacata aggggagagg ttgtcggcga ccgaatcctc gccgtagaag
     1741 aacatcgaat acatccagag ggcggactcg cgctgcagct cgctgagcga atcccagccg
     1801 gcccggtcgg ccgagaaatc gagggacatc gcgctccagt tgttctcttc ccagcggcgg
     1861 tagaggtcca tgtaggaaat ggacccggcg ctgtttcctc ttggttcggc ctgggcgacg
     1921 gacatcgact ctctcctctt ctcggattcg gtccccgata ctaactgact cgtcagtcag
     1981 ttactagaat cggagtcgtg aacgagagcg gagcaggcca gagcgaggac cagccggcgg
     2041 tacgtcgccg gatgccggcg gccgaacgcc gcaaactgat ccttgacgcc gccctcgatg
     2101 cattcgccga agggggttac gaagaaacga cgctcgacga ggtggccgag agggcggggg
     2161 tgtccaaggc actgatctac gagcatttcc cgtccaagcg ggatctctac cgggagatcc
     2221 tcgaaacatg ggtgggagaa ctgctcggcc gggttctggc ggcggccacc ggggcgggcg
     2281 agcgaagcga agaccggctg gtggccggcc tggacggatt cctgatcttc gtcgaggagc
     2341 gaagggacgc ctggcggctg ctgatccggc accgggcaag cgacgacgtc tctgagacct
     2401 ttgaccggct cttcgaggaa gtcgcgagga tcatcggtgc cctgatggcc ccggagatgc
     2461 cgaagtccgt gacgcccgag ggggttgact tcgacctggt tctggatgcg acctcaaggc
     2521 agttgatggg ggcggtgacc gcgatcgcca actggtggga cgaacatcgc gaagttccgc
     2581 gggaccgggt actggcgatg gtgatggagt tcgcctgggt cgggatggag cgggccgcag
     2641 ccggcgaaag ctggtcgggc ggtcactgac cgggatcgtg cggcgatccg tcgccggcac
     2701 tcacggtgtc caccgcctgg tcgatcgaga cataggaatc cgccagaagg ccggccagcc
     2761 cggttgcccg catcatgtcc tccacctggc cgacgtccct ggccacggcc agccggatct
     2821 ggcgccgtct gagttcttcg tagagagacc tgagcatgta cacgccggtc acgtcgatgt
     2881 aggggacggt ttccacgtcg agcaccactg ccctggtgtc tggccggatg ctgccgatta
     2941 cttccttccg gatctcttcg gcattggcga agaagatcgc cgcttcgagt ctcaggacca
     3001 ccgtgcctgg cacctcaagg gcatcggggt gacgccgccg gtcaccccaa tgggttggct
     3061 gcgacttcaa ctcgccgagc tcggccaccc gcggacggga agaacggtag agcagcagga
     3121 ccagcgaaac gccgattccg atgaacagcc cgggcagaat gtcgaagacc atcaccccgg
     3181 ccagcgccgc gatcgacgcg atgaaatccg gacggttggc cggattcagg ggattgccgc
     3241 gttccctgat cctggccagg tcccgcaggc cgacgatgtc gacggcgtga atcagggcgg
     3301 cgatcaccac cgccgccagg gcggcttccg gcagatcctc gaagagcccg gtcaggaaga
     3361 gcatggtcag gatggccagg acggctacga cgattccgga cagctggctg cgcgctccgg
     3421 aatcggcgtt gatcgaagtc ttggaaaaac tgccggtgac cacgaatccg ccgaacagcc
     3481 ccgctcccac gttggcggcg ccggtcccga ccagctcgcg gttcgggtcg aggctctcct
     3541 cgctgtccgg ataggccttg gcggaggcga ggctctcggc gaagccgacc agcatcaggc
     3601 cgacggcccc cgcgacaagc ggcccgagat cggccagccc gatgtccggc agggagatgg
     3661 aggggagatc cgagttgacc tgcccgacga tttctacgcc gtgggcgccg agatcgaatg
     3721 cattgactgc cgcaactccg aggaccaccg cgatcagcga accgggaacg gccggtcgac
     3781 ggtatttgca gaaaaggatg atcgccaggg aaatcagacc aatcgccacg gtcggcaggt
     3841 caacctggcc gaggtgggtg aggaagaccc agatccggtt gaagaagttg ccgttgctcg
     3901 actcgactcc gaacagatac gggatctggc cggcgatgat ggtcagggcg agaccgacga
     3961 tgaacccgcg caggaccggg atcgagatga agttgaccac gaagccgaga cgggcgatgc
     4021 ccgcagcgaa ggcgatgacg ccgacgcaga tcgcaagggc agccgtggtc tgcacgaagg
     4081 ccgagctgtc cccggtgacg atgccgccga cgatcgcggc agacagcgcc gcggtcgcgg
     4141 cgtcggcgcc ggtcaccagc cgccgggatg aaccgagcgc cgcatagatg agtagcgcgc
     4201 cgggagccgc gtagaggccg acgatcggcg ataccccggc gatcgtcgcg taggcgagcg
     4261 cctccggtac cagcaccgcc caaacggtca gggcggcgag cagatccctc ctcagccacc
     4321 ccctcgaata aggccggatc gaggggaaga gcttcaaagt ccgagccgct cctgcgccga
     4381 tcgcacctct ttcagcgcgt cctcgctcga catgcccaga ctcagcagga gatcggtcgc
     4441 ggccgagcgg atctgtccga cgatcacgct gaccgagagg tttgaggtct cttccagcgc
     4501 cgcggtggcc ttggcggcgg cgcgcaatgc cgattcgcgg gtggccgacc tggccggggc
     4561 cccgcccagg tgctggtcga gcctcgccac ggccgtcgcg aggtcccgga tcgagtcgga
     4621 caccaccggg ggaatgtttt cgtcgaggtc gatcgcccgc tggcagcccc gggccagaac
     4681 ccgggtattg cggatcatca ggtcgatcgg ctcggctgcc gccgcaagct gatcgaccct
     4741 tcccctgaat cttcgccgcg tcggggcgag cgagatggtc tcctgcccgg cctccacggc
     4801 gatcttcatt tcccgtaccg ccgggtcgag tccgcgggcc ctctcgagtg cggccacgat
     4861 ctcctcatgg ctgttgtggt ccagggcatc ggcgatgtcg accagcgttc cagagagcct
     4921 ggaaaggagc ggttcgaggt agcgccgcac cagccggacg ggatcgaccg gagtgatcgc
     4981 attggcggtc agggccacac cgccgccgac cagcgcgtca atgaagcgat gggttgaatc
     5041 gaccaggtcc ggagccggca gggtggcgac caggacagcg gatatcgccg cctggttgac
     5101 cagcagcacc ccgccaccgg ccagcaccgc ggcggagacg gcaa
//
LOCUS       DAOTIP010000042         5139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_19205_length_5139_cov_3.365657, whole genome shotgun sequence.
ACCESSION   DAOTIP010000042 DAOTIP010000000
VERSION     DAOTIP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19205_length_5139_cov_3.365657"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="P5138_01430"
     CDS             <1..1016
                     /locus_tag="P5138_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV59268.1"
                     /translation="TTGQPKGAELTHANIRSNLESIKDLFTPTEEDVFFGGLPLFHVF
                     GQTVAMNAAIKTGAELTLLPRFDPSKALEIIQRDRVTIFQGVPTMYAAMLQVPNRADY
                     DISSLRLCVSGGAALPVEIIRAFESEFNTPILEGYGLSETSPVASFGRVDMERKPGTI
                     GTPIKGVELRIVDEEGNVLGVGDVGELQIKGPNVMKGYWEMPEATSKAIDSEGWFSSG
                     DMATVDDDGYYSIVDRKKEMILRGGYNVYPREVEEVLYEHPAVAEAAVMGVPHDDLGE
                     EIVAIIAFKEGESATEDELREHAKSGVAAYKYPRHIVIVDELPKGPTGKILKREIDLT
                     GVA"
     gene            complement(1063..1512)
                     /locus_tag="P5138_01435"
     CDS             complement(1063..1512)
                     /locus_tag="P5138_01435"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV59269.1"
                     /translation="MDRETVIRELRLEPLPKEGGMYRRKFKDENSSSIYFLIEPDSPT
                     KLHRLPWPELFHFYAGAPARIHILGPEPGVARHPVLGIGLEEGERPQMLVPGGTWQAA
                     ETTGAWTLLGTTMAPAFDWDRFELGKRDRLLKYWPDQEEVILRHLDD"
     gene            complement(1551..2525)
                     /locus_tag="P5138_01440"
     CDS             complement(1551..2525)
                     /locus_tag="P5138_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59270.1"
                     /translation="MTGARQLAVLGLCLGLILAGCGSSDDEATVSKAPDKSGQISQLQ
                     KQVKRLEKRDKARQKREAAAAEAKTARVGGDVDANRILAGLPGEAGLVVGAPGGGSPD
                     FSGGSFTTGDAWSTIKVPIAERVLDDFGGPARISSTQKANITSAITLSDNDAAAALFA
                     DLEAKHGGLEGASDAVGEMLRQAGDDETVISTQGRDSFSTYGQTDWSLANQFKYMAAL
                     AGGCISNSANRSYLLGQMASVSGSDTYGLGSAGFPAKWKGGWGPGTDGKYLVRQMGVM
                     EVDGKQVVVAMAAIPDDGSFESGMSMLNDVARSVATRLADQVSAPTGC"
     gene            complement(2522..3031)
                     /locus_tag="P5138_01445"
     CDS             complement(2522..3031)
                     /locus_tag="P5138_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59271.1"
                     /translation="MSRIMGVFAVLVLVLAGAFAVGCGGSDDGATQEQLDAAKEEGAK
                     EARQQAKLQQLQDQVKELQNQAQNQNSSGSNSGGSSGSSTGGSGTSSSTTGTGPVSSC
                     TDGVQVGSNTSCAFAMNVAGEYGSNPGATSIRAYSPVTGEYYVLACGPWTGGGTVCVG
                     GNGASVYLP"
     gene            3114..>5139
                     /locus_tag="P5138_01450"
     CDS             3114..>5139
                     /locus_tag="P5138_01450"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRV59272.1"
                     /translation="MRRIRIAVAGAAMICAAAILPSVASADCTLTSQYNGNDVSTLTG
                     GTTQTFSGHFDPDLNGKFIQIPFEVGSGMTGMKIRYCFAKSNAADDSPTLDLGVYGAK
                     PTGQTGWTQAQRRGWSGSAVRTIGIGENGYTSDTVYNENSPSERKYYTPGYTTRAYKP
                     GPIEAGEWAVELGAGWIDPGGSGVDWKVEVKTSTDPAWKSENSGQGFIADPYTPYVAN
                     PNAGWYTGDMHVHGEMEPGNATMKQTMDLGFGDLPTGNGLDFMTLVDHNNENSRNLLG
                     KSTYSYPGKLVIPGIEVTTYNGHWNATGSSNFADFRFSPVYWWDDGSGGPDDDQETAD
                     ELKLVRGAIEPASQMQPIIDGGGFTQVNHPETLKNAPASCRGCAWTYTDEQTDWSKVN
                     ALEIQNGAAGIPFANPTGMNTFTVASISTYERLLADGFHIAAVGSSDDHQAGESTGIF
                     DGQVGRGATVVHATQLSTQGIVDAVKAGHTYVKPFGADAPDVDFSATTPEGGSGIPGD
                     SLTGSGLNVSINVKGAMSSVRPGTYTLKLLQDGIEIDSAQVTSDDFDFSKTVTESGRY
                     SFELTRAQDAATMIEGYSTPVWFTFKAAAKPSNAFSFGAYKANRKKGTATLKVKVKSP
                     GKVTLTGSGLNKATAKVKKKNQTVTLNLRPKSSLKKKLKKKGSAKVKVKVT"
BASE COUNT          980 a         1716 c         1592 g          851 t
ORIGIN      
        1 gcaccaccgg ccagcccaag ggtgccgaac tcacccacgc caacatccgt tcgaacctcg
       61 agtcgatcaa ggacctcttc accccgaccg aggaggatgt cttcttcggc ggtctgccgc
      121 tcttccacgt cttcggccag accgtggcga tgaatgccgc gatcaagacc ggcgccgagc
      181 tgaccctgct gcccaggttc gacccgtcga aggccctgga gatcatccag cgcgacaggg
      241 tgaccatctt ccagggagtt ccgaccatgt acgcggcgat gctgcaggtg ccgaaccgcg
      301 ccgattacga catcagctcg ctccggctct gcgtctccgg cggggccgcc ttgccggtcg
      361 agatcatccg cgccttcgag agcgaattca acacgccgat cctcgagggc tacggcctct
      421 cggagacctc ccccgtcgcc tccttcggga gggtcgacat ggagcgcaag cccggcacga
      481 tcggaacccc gatcaagggt gtcgagttgc ggattgtcga cgaggaagga aacgtgctcg
      541 gcgtcggcga cgtcggtgaa ctccagatca agggcccgaa cgtgatgaag ggctactggg
      601 agatgcccga ggcaacgagc aaggcgatcg actcggaggg ctggttctcc tccggcgaca
      661 tggcgaccgt tgacgatgac ggctactact cgatcgtcga ccgcaagaag gagatgatcc
      721 ttcgcggcgg ctacaacgtg tacccgcgcg aggtcgagga agtgctctac gagcatcccg
      781 ccgtggccga ggctgccgtg atgggtgtcc cccacgacga tctcggcgaa gagattgtcg
      841 cgatcatcgc cttcaaggaa ggcgagtcgg cgaccgagga cgaactccgc gagcacgcaa
      901 agtccggggt tgccgcctac aagtacccgc ggcacatcgt gatcgtcgac gagctgccca
      961 agggtccgac cggaaagatc ctgaagcgcg agatcgacct gaccggcgtc gcctgagccg
     1021 actccggacg tgagccggga ccggacctga tccggttccg gcctagtcgt cgaggtggcg
     1081 gaggatcact tcctcctgat ccggccagta cttgagcagc cggtcacgct tgccgagctc
     1141 gaagcggtcc cagtcgaagg cgggggccat cgtggttccg agcagggtcc aggcaccggt
     1201 cgtctctgct gcctgccagg tgccacccgg cacgagcatc tgggggcgct ctccttcctc
     1261 gagaccgata cccagcaccg ggtgtctggc cacgcccggc tcgggaccga ggatgtggat
     1321 gcgcgccgga gcgcccgcat agaagtgaaa gagctccggc cagggcagcc ggtggagctt
     1381 ggtcggtgag tccggctcga tcaggaagta aatcgagctc gagttttcat ccttgaactt
     1441 cctccggtac atgccgccct ctttgggcag cggctcgaga cgcagttcac gaatgaccgt
     1501 ttcgcgatcc ataccctgac attacgcgag atgcccgacg acggtgttcc tcaacagcct
     1561 gtcggtgcag aaacctgatc ggccagacgg gtggcgaccg agcgggcgac gtcgttcagc
     1621 atcgacatcc cggactcgaa ggagccgtcg tccgggatcg ccgccatggc gacgacgacc
     1681 tgcttgccgt ccacctccat cacgcccatc tgcctgacca ggtacttgcc gtcggttccg
     1741 gggccccagc cccccttcca cttggccggg aacccggccg aaccgagccc gtaggtgtcg
     1801 ctgccgctga ccgaagccat ctgaccgagc aggtaggagc ggttggccga attgctgatg
     1861 cagccgccag ccagggcggc catgtacttg aactggttgg ccagggacca gtcggtctgt
     1921 ccgtaggtcg agaagctgtc ccttccctgg gtggagatca ccgtctcgtc gtcgccggcc
     1981 tggcgcagca tttcgccgac cgcgtcggag gcgccctcca gccctccgtg cttcgcttcg
     2041 agatctgcga agagggcggc cgccgcgtca ttgtcggaga gggttatcgc gctggtgatg
     2101 ttcgctttct gggtggagct gatccgggcc ggaccgccga aatcgtcgag cactctctca
     2161 gcgatcggaa ccttaatcgt cgaccatgca tcaccggtcg tgaacgagcc accgctgaag
     2221 tcgggagagc ctccacccgg agccccgacc accagcccgg cctcgcccgg aagcccggcc
     2281 aggatcctgt tcgcgtcaac gtctccgccg acccgggcgg tcttcgcctc cgctgcggcc
     2341 gcctctctct tctgtctcgc cttatctcgt ttctcgagcc gtttcacctg tttctggagc
     2401 tgcgatatct ggccgctctt gtccggagcc ttgctgacgg tcgcttcatc gtccgaggat
     2461 ccgcagccgg ccaggatcag gccaagacag agtccgagaa ccgccagttg acgggcgccg
     2521 gtcaaggcaa gtagaccgag gcgccgttac cgccgacgca gactgtcccg cccccggtcc
     2581 agggaccgca ggcaagcacg tagtactcac cggtgaccgg gctgtaggcc ctgatcgaag
     2641 tggccccggg attgctgccg tattcaccgg cgacgttcat cgcgaaggcg caggaggtgt
     2701 tcgacccgac ctggacgccg tccgtgcagg acgagaccgg gccggtgccg gtggtcgatg
     2761 aggaggtacc ggaaccgccg gtcgaagacc cggaactgcc tccggagttg cttccggagc
     2821 tgttctggtt ctgggcctgg ttctgaagct ctttgacctg gtcctgaagc tgctggagct
     2881 tggcctgctg gcgggcttcc ttggcgcctt cctccttcgc cgcgtccagc tgctcctggg
     2941 ttgcgccgtc gtcactgccg ccgcaaccga cagcgaaggc accagccagg accagaacga
     3001 ggaccgcgaa cactcccatg attcgagaca tgaccgaagc ttatccgcgc ccggcgggtt
     3061 cttgaaggac ccgcctcctg cggatactgc cacctgacga atattctgga gccatgagga
     3121 gaatccgcat cgccgtagcc ggcgccgcca tgatctgcgc cgccgccatc ctgccctccg
     3181 ttgcatctgc cgactgcaca ctgactagcc aatacaacgg taatgacgtc agcaccctga
     3241 ccgggggaac cacccagact ttctccggac atttcgatcc ggacctcaac ggcaagttca
     3301 tccagattcc ctttgaggtc ggaagcggca tgaccggcat gaagatccgt tactgctttg
     3361 cgaagtcaaa cgcggccgac gacagcccga ctctggatct tggcgtctac ggagccaagc
     3421 caacgggaca gaccggctgg acccaagccc agcgccgcgg gtggagtggc agtgcggtgc
     3481 gcactatcgg catcggcgaa aacggctaca catcagacac cgtttacaac gagaacagtc
     3541 catccgagcg caagtactac acgcccggct acacgacacg cgcctacaag cccgggccga
     3601 tcgaggccgg cgagtgggcg gttgagctcg gtgccggctg gatcgacccg ggcggttccg
     3661 gtgtcgactg gaaggttgaa gtcaagacca gcacggatcc ggcgtggaag tccgagaaca
     3721 gcggccaggg cttcatcgcg gatccctaca cgccctatgt cgccaacccg aatgcgggct
     3781 ggtacacggg cgacatgcat gtccacggcg agatggaacc gggcaacgcc acgatgaagc
     3841 agacgatgga cctcggcttt ggtgacctgc cgaccggcaa cggcctcgac ttcatgaccc
     3901 tggtcgacca caacaacgag aactcgcgaa acctgctcgg caagagcact tactcctacc
     3961 ccggcaagct ggtcatcccc ggcatcgagg tgaccaccta caacggccac tggaacgcaa
     4021 cgggatcgag caacttcgcc gacttccgct tcagcccggt ctactggtgg gacgacggtt
     4081 ccggcggccc ggacgacgat caggaaactg cggatgagct caagctggtc cggggagcga
     4141 tcgaacccgc ctcccagatg cagccgatca tcgacggggg cggtttcacc caggtcaacc
     4201 acccggaaac cctcaagaac gcccccgctt cctgccgcgg ctgcgcctgg acctacacgg
     4261 acgagcagac tgactggagc aaggtcaacg ccctcgaaat ccagaacggt gcggccggca
     4321 ttccgttcgc caacccgacc gggatgaaca ccttcaccgt ggcctcgatc tccacctacg
     4381 agcggctgct tgcggacggt ttccacatcg ccgccgtcgg ctcgagcgac gaccatcagg
     4441 cgggagagtc caccggaatc ttcgacgggc aggtcggaag gggagccact gtggtccacg
     4501 ccacccagct ctcgacccag gggatcgttg atgcggtcaa ggccggtcac acctacgtga
     4561 agccgttcgg cgccgatgcc cccgacgtcg atttctcggc cacgactcct gaaggcggct
     4621 cgggcatccc gggggattca ctcaccggct cgggcctgaa cgtctcgatc aacgtgaagg
     4681 gagcgatgag ctcggtccgt cccggcacct acacgctgaa gctgctccag gacgggatcg
     4741 agatcgactc ggcccaggtg accagcgatg acttcgactt cagcaagacc gtcaccgaat
     4801 ccggccgcta ctccttcgag ctgacccgcg cgcaggacgc ggcgacaatg atcgagggct
     4861 actcgacccc cgtctggttc accttcaagg cagccgcgaa gccctcgaac gcgttcagct
     4921 tcggtgccta caaggccaac cggaagaagg gcaccgcgac cctgaaggtg aaggtcaaga
     4981 gcccgggcaa ggtcaccctg accggttccg gcctgaacaa ggccacagcc aaggtcaaga
     5041 agaagaacca gaccgtgacc ctgaacctgc gtcccaagtc ttcgctcaag aagaagctga
     5101 agaagaaggg ctcggccaag gtcaaggtca aggtgacca
//
LOCUS       DAOTIP010000043         5116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_19329_length_5116_cov_3.334519, whole genome shotgun sequence.
ACCESSION   DAOTIP010000043 DAOTIP010000000
VERSION     DAOTIP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19329_length_5116_cov_3.334519"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="P5138_01455"
     CDS             complement(<1..705)
                     /locus_tag="P5138_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRV59273.1"
                     /translation="MTAAQAASASYPISDYFDEAFSPDGELRAAYRGLLDGLDSTGLA
                     GIQGHVQSRLREDGVEFGPEGKRSAFVVDAVPRIITAAEWSILEAGLCQRIRALQMFL
                     ADIYSRQEIITAGIMRRSILDGAPHYEPVAAGFGTPVVADIAGPDLVRDESGFRVLED
                     NLRFPSGITFALVARRAVGTGWKTGLEPVEAESAFGLLGEMLRNRAPEGDGDPVIAIL
                     SEGPDSAAFFEHRTLAT"
     gene            complement(842..2083)
                     /locus_tag="P5138_01460"
     CDS             complement(842..2083)
                     /locus_tag="P5138_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59274.1"
                     /translation="MPEAIRRSSAQGAGGKREIVVRNSRFAVASRYQLIGLGLLVAVL
                     LALVALPGRSQAATEPFKIDFDQSTMQVGLLSDLPLGELASTASLEGTIDDQGNVTVP
                     KGKFTLPEIGITDPLTVKMFMGIESDATGTFNAQTGALVLNAKAGVWVSVNLPELFDA
                     LDTLGLDVSGQLGPLAGIIGGVGDLTCGFSPMDVTFTTGETSLGSGSPFTKGPLGPGA
                     LTAEWSQLGPFSGKTKILGLIDVCQAIKQYAPSLIEGALSGSLPDGIDLGNIDLASLL
                     NNLDNVNLGPSSLTLTRTLDENVPEVVPPASPALKLSVSPKRRKARAGKKASFTVKVK
                     NSGKAPATGVKICVKTPGASVVGGGRCRTLGKVMAGATQTRRFRIRTGKKRKKPVKAV
                     FTVRASNAGRSSASARLLIRH"
     gene            2261..4651
                     /locus_tag="P5138_01465"
     CDS             2261..4651
                     /locus_tag="P5138_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRV59275.1"
                     /translation="MRSIRVYLAVLAAVISGLALIGGMATTASAKSKKLKTAPKITST
                     APYKARGSVGDAYVKNATPGTKLLLVNAKNKIVRTGKADSYGSKVFYDVAPGKVFTVR
                     TPKGKGSQGTRKFKVLKPGANPPESWYQSKPALHQGLNYVTMRDGTELAMTVRLPSGK
                     SLADGPFPTYIEYSGYQTAAPHDLLNAFLGSGGSDPLTPASSTAVGSVIGPLLDFATV
                     SVQMRGSGCSGGAFDLFGWPTTYDGYDAVETVAAQDWVAGGNVSMGGISFSGITQLFT
                     AGTRPPHLAAVSPLSVTDDVYYGTGFPGGIFNNGFAFSWITERAEDAEPAPEGGQSWA
                     KVMTTTGDPDVAEPLRSEQIQHCVDNQKMRLQTRNYNNLIETNPYRTQQLFQKRSPGA
                     WVKKINVPIFWVGAFQDEQTGGHWPEAVKNLPKSNKDVWVTMQNGVHVDSLGPSTITR
                     WAEFMNLFTGNGRIPKLSPLVISASSLLYDEVADAGALPIEQSRYASMNNTPTNVAAA
                     KTDFRKDPRIRILMDNGAAVDGDPGAIGALWEADFGNWPINSTKPTSYYLGKNGTLSR
                     TKAKKGSAVSYKSDPSARPAKTLGENAQGDSWLAQPPYQWEPVAAGKGLGFITPPLSK
                     DVFMAGPSSVDLYLKSSKANTDVQVTLTEVRPDGNETYVQNGWLRATHRKLNKKLSTA
                     YDPVQTWQKKDAKYLKKGKYNYLRVQIYPAAHVFRAGSRIRLNIQAPGGDRTIWDFDT
                     IEKGKTKNTIGLGGVIPSKLVLPVIPGEDAQGTPLPGPTALRGEPNRTYQPASNGG"
     gene            4837..5022
                     /locus_tag="P5138_01470"
     CDS             4837..5022
                     /locus_tag="P5138_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59276.1"
                     /translation="MRRIHVSLAGLFTIVLGLLVLGAATSPAGAAGKKPKRAPKITSK
                     ANYTARGSVGDAYVKNA"
BASE COUNT          981 a         1674 c         1609 g          852 t
ORIGIN      
        1 tgtggccagc gtgcgatgct cgaagaaagc ggcactgtcg ggcccctcgc tcaggattgc
       61 gatcaccggg tcaccgtccc cttccggtgc ccggttccgc agcatctcgc ccaacaggcc
      121 gaaagcgctt tcggcctcga ccggctcgag tccggtcttc catccggtcc cgaccgccct
      181 ccgggcgaca agcgcaaagg tgattcccga gggaaagcgg aggttgtctt ccagcacgcg
      241 gaagccgctc tcgtcccgga ccaggtcggg tcccgctatg tcagccacga ccggcgtgcc
      301 gaagccggct gccaccggct cgtaatgggg tgccccgtcg aggatggaac ggcgcatgat
      361 tccggccgtg atgatctcct gacgggaata gatgtcggcg aggaacatct gcagggcccg
      421 gatccgctgg cacaggccgg cctcgaggat ggaccattct gccgcggtga taatccgcgg
      481 aaccgcatcc acgacgaatg cggagcgctt tccttcgggg ccgaattcca ccccgtcttc
      541 cctcaggcgg gactggacat ggccctgtat cccggccagt cccgtcgagt cgagcccgtc
      601 aagcagccca cggtaagccg cacgcaactc gccgtcgggc gaaaaggcct cgtcgaaata
      661 gtccgaaatc ggataactgg cactggcagc ctgcgcagcc gtcatggcta aacgataagc
      721 cgggagcggt catcgaacat ttggcccgcg gtcgaaactg agggctgtcg tcctcgccaa
      781 cccagaatcg ggccgattcg aagggcgcgg gatctttgcc gggatccgct ttcccgccgg
      841 ctcagtggcg gatcagcagg cgggccgagg cgctcgatcg cccggcattt gaagcccgga
      901 ccgtgaagac ggccttgacc ggcttcttgc gcttcttgcc ggtcctgatc ctgaaacgcc
      961 gggtctgggt ggcgccggcc attaccttgc cgagcgtccg gcagcgcccg ccgccgacca
     1021 ccgatgcccc tggcgtcttc acacagatct tgactccggt ggccggagcc ttgccgctgt
     1081 tcttgacctt gaccgtgaag gaagccttct tgccggccct ggccttgcga cgcttcggtg
     1141 agaccgagag cttcagcgcc gggctggcgg gagggacgac ctcgggcacg ttctcgtcca
     1201 gggtcctggt cagggtgagt gagctcgggc cgaggttgac gttgtcgagg ttgttgagca
     1261 gcgaggcgag gtcgatgttg ccgagatcga ttccatccgg caacgacccg ctgagcgccc
     1321 cttcgatcag gctgggggcg tactgcttga tcgcctggca aacgtcgatc aggccgagaa
     1381 tcttcgtctt cccggagaac ggtccgagct gggaccactc cgccgtgagg gcgccgggtc
     1441 cgagcgggcc cttcgtaaac gggctgccgc tgcccagcga ggtctcgcct gtcgtgaagg
     1501 tcacgtccat cggggagaag ccgcaggtca ggtcgccgac cccgccgatg attccggcga
     1561 gcgggccgag ctggccgctg acgtcaagtc cgagggtgtc gagcgcgtca aacagttcgg
     1621 gcaggtttac cgacacccag acgcccgcct ttgcgttcag caccagggcg ccggtctgtg
     1681 cattgaaagt gcccgtcgcg tcactctcga tccccatgaa catcttcacg gtcaacggat
     1741 cggtgatccc gatctccgga agcgtgaact tgcccttggg aacggtgacg ttgccctggt
     1801 catcgatcgt gccttcaagg gaagcggtcg aggcgagttc acccaggggc aggtcgctga
     1861 gcaggcccac ctgcatggtg ctctggtcga agtcgatctt gaaaggctcg gtggctgcct
     1921 gggaacgacc aggcaaggcg accagggcca ggaggaccgc gaccagcagg ccgagtccga
     1981 tcagctgata cctcgaagca actgcaaacc gcgagttccg caccactatc tcccttttcc
     2041 cgcccgctcc ctgggcacta gatcgccgga tcgcttccgg cactgatcac actataaacc
     2101 cgacccccga tctcccgggt tcaacacctg agaaaccctg ccgttcacgg aatccgcgaa
     2161 atcccttata gtttcctgag cagggagtgg aagaaggact tccacgggag acagggacgt
     2221 gctcccggga ctcgtcaatt agggatggag gaaacgcagc atgcgcagca ttcgggtgta
     2281 tctggccgta ctggccgcag tcatttcagg gctcgccctg atcggtggca tggccacaac
     2341 cgccagcgcc aagtcgaaga agctgaagac ggcaccgaag atcacttcga cggcgcccta
     2401 caaggctcgc ggcagcgtcg gtgacgcata cgtgaagaac gcgactccgg gcaccaagct
     2461 tctgctcgtc aacgcgaaga acaagatcgt gcggaccggc aaggccgact cgtacggctc
     2521 gaaggtcttc tatgacgttg cccccggcaa ggtcttcacc gttcgcaccc cgaagggcaa
     2581 gggttcccag ggcaccagga agttcaaggt cctgaagccc ggtgccaatc ctccagagtc
     2641 ctggtaccag agcaagccgg ccctccatca gggtctcaac tacgtgacga tgcgagacgg
     2701 aaccgaactg gcgatgaccg tccggcttcc ttccggcaag tcgctggccg atggtccttt
     2761 cccgacctac atcgagtact ccggttacca gaccgccgct ccccatgacc tgctcaacgc
     2821 attcctcggc agcggaggaa gcgatccgct gacccccgcg agttcaaccg cggtcggttc
     2881 ggtgatcggc ccgctgctcg actttgcgac ggtgagcgtc cagatgcgcg gttccggctg
     2941 ctccggcggc gccttcgacc tgttcggctg gccgaccact tatgacggct acgacgcagt
     3001 cgagaccgtc gcggcccagg actgggtcgc gggcggcaac gtgagcatgg gcggcatctc
     3061 cttctcgggg atcacccagc ttttcaccgc cggcacccgg ccaccgcacc tcgcggccgt
     3121 atccccgctt tcggttaccg atgacgtcta ctacggcacc ggcttcccgg gaggcatctt
     3181 caacaacggc ttcgcgttca gctggatcac ggaacgcgcc gaggacgccg agcctgcgcc
     3241 cgagggcggt cagtcctggg ccaaggtgat gaccacgacc ggtgacccgg acgtggcgga
     3301 gccgctccgc agcgaacaga tccagcactg cgttgacaac cagaagatgc ggctccagac
     3361 ccgcaactac aacaacctga tcgagaccaa cccgtaccgc acccagcagc tgttccagaa
     3421 gcgttccccg ggtgcctggg tcaagaagat caacgtgccg atcttctggg tcggtgcctt
     3481 ccaggacgag cagaccggcg gccattggcc cgaggctgtg aagaacctgc cgaagagcaa
     3541 caaggacgtg tgggtgacca tgcagaacgg cgttcacgtc gattcgctcg gtccgagcac
     3601 gatcacacgc tgggccgagt tcatgaatct gttcaccggt aacggccgga ttccgaagct
     3661 ctccccgctg gtgatcagcg cgagctcgct gctgtacgac gaggtcgccg atgccggtgc
     3721 cctgccaatc gagcagagcc gttacgcgtc catgaacaac accccgacca acgtggcggc
     3781 ggccaagacc gatttccgca aggacccgag gattcggatc ctgatggaca acggcgcggc
     3841 cgtggacggc gatccgggag cgatcggtgc cctctgggag gccgatttcg gcaactggcc
     3901 gatcaactcc accaagccga cctcgtacta cctcggcaag aacgggaccc tgagccgcac
     3961 caaggcgaag aagggctcgg cggtcagtta caagtccgat ccgagcgccc gtccggccaa
     4021 gaccctcggg gagaacgccc agggtgactc ctggctggcc cagcccccct accagtggga
     4081 gccggttgct gcgggcaagg gtctcggctt cattacgccg cccctgagca aggatgtgtt
     4141 catggccggt ccgtccagcg ttgacctcta cctcaagtcg agcaaggcca acaccgacgt
     4201 tcaggtcact ctgaccgagg tccggcccga cggcaacgag acctatgtcc agaacggctg
     4261 gctgagggca acgcaccgga agctgaacaa gaagctctcg accgcgtacg acccggttca
     4321 gacctggcag aagaaggacg ccaagtacct gaagaagggc aagtacaact acctcagggt
     4381 ccagatctac ccggccgcgc atgtcttccg tgccggctcg aggatccgtc tgaacatcca
     4441 ggcgccgggc ggtgaccgga cgatctggga cttcgacacg atcgagaagg gcaagacgaa
     4501 gaacacgatc ggcctcggcg gcgtgattcc gtcgaagctg gtcctgccgg tgatcccggg
     4561 cgaggacgcc cagggcacgc cgctgccagg cccgaccgcg ctccgtggcg agccgaaccg
     4621 gacctaccag cccgccagca acggaggctg atgtagttcc acatgcattg aggacaaagg
     4681 gccgcccggg agggtggccc tttgtcgtta agttgccccg ctcgggaaac agcctgttta
     4741 tgagtcaggg attcggggaa agaagggtca atcctcgaaa ccggtgcgcg agaggcgcgc
     4801 cacgactcaa cacttcaaag ggaggtttct cgcaacatgc gcaggattca tgtgtctctg
     4861 gcaggcctgt tcaccatcgt cctcgggctg ctcgtccttg gtgcggccac atctccggcc
     4921 ggcgccgcag gcaagaagcc gaagagggcc ccgaaaatca cctcgaaggc gaactacacg
     4981 gcccgcggga gcgtcggcga tgcctacgtg aagaacgcgt agcccggcca gagactgctg
     5041 ctggtcaacg cgaaaaaccg catcgtccgt cgcggcaagg cggaccggtt cgggtcaaaa
     5101 atcttctacg acgtga
//
LOCUS       DAOTIP010000044         5075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_19532_length_5075_cov_3.722112, whole genome shotgun sequence.
ACCESSION   DAOTIP010000044 DAOTIP010000000
VERSION     DAOTIP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19532_length_5075_cov_3.722112"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..467
                     /locus_tag="P5138_01475"
     CDS             252..467
                     /locus_tag="P5138_01475"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV59277.1"
                     /translation="MSNWNMMTLTAQIHRENGAYWADIPDLPGVFATGDTLDELFDSL
                     EEGVSSVLESESGSGTVNVNGALISIS"
     gene            complement(520..1965)
                     /gene="purF"
                     /locus_tag="P5138_01480"
     CDS             complement(520..1965)
                     /gene="purF"
                     /locus_tag="P5138_01480"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRV59278.1"
                     /translation="MTERIDLDREGPRDECGVFGVYAPGHDVSRLAYFSLYALQHRGQ
                     ESAGIATCEGGHITTMRDGGLVSQVFDEDKLRALTGDMAIGHVRYSTTGGGSWENAQP
                     IWRDDGRELALAHNGNLTNAVGIYNRLRRKGLEFRGTTDSEIIAALISSDYGRHVEDA
                     VANVMPQLEGAYSTVVMTKHAIVAFRDPYGIRPLSLGQLPDGSYVFASESCAFDIIGA
                     KLVREVSPGEMISLSERGLESRQVIKSERTAMCVFEHIYFSRPDTKLEGKRLQEVRGQ
                     MGEILWREAPADADLVISVPDSGTPAARGYARASGLPQDDGLIKNRYVARTFIQPGQE
                     LRKHGLRMKFNPLPEIISGKRVVVVDDSIVRGNTTRQIVGMLRDAGAKEVHLRISAPP
                     IRFGCNYGVDMSARDEMVAHERTIEEIAEHIGADSLAYLSMEGVYEAVGTPAEVHCDA
                     CFTGNYPLGDDPDEADGKFDLEQIAVIPATR"
     gene            2123..3094
                     /locus_tag="P5138_01485"
     CDS             2123..3094
                     /locus_tag="P5138_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59279.1"
                     /translation="MVKSRGIRGSGFIALTALLAVALLAMPADSGAATKKQRQCQRAI
                     TSVFTFKKRVPKRKRKKAVRIICQTMTIGKTGPKGATGAKGTTGPIGPLGSTGPIGPT
                     GTTGATGAIGLPGVPGPTGATGIQGLIGPTGPIGITGPTGVTGLIGEIGPTGPTGLTG
                     LIGPTGATGLTGALPMVFASIPSSSPQVMNDSVNTVTGWTDEYDPSGTFDASSGTFTA
                     PHSGYYLIEPEITTGPASAVTATGGQPPVLITEVNGLGEDIQDFPLFNTDLTLLTMLV
                     PLQTAQVSGESLQQLSSDDEVVIQVIKQDGIDYDTYGDLKITELPAP"
     gene            complement(3139..>5075)
                     /locus_tag="P5138_01490"
     CDS             complement(3139..>5075)
                     /locus_tag="P5138_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59280.1"
                     /translation="VPLGRRAAVVNYRIRFFLRLIPIAASLMLLGPAGQALAAPANDN
                     FANAQALTGTLPITVTGNNIGATAEPGEPDTYSIPPLSSVWFKWVAPAETDAVLDFCH
                     ANFSGGNENDSPVMAIRSGVTLGTLVSVKETNGPCSLRFHANAGVTYNFQIDWYDKEG
                     TFDLTLRKLTPPANDNFATPTNIGPALPIEANGTTVDSNWEAGEPGTLGGSSNSRSVW
                     FRWTAPATERVRLEACEAELVDGSLNEVAAIYTGATLGTLVEVAKNTSNDCAVDFPVV
                     AGTTYRIAVSGHFGGEFTFVLRLKSAPPPTNDNFANAQVVGPNLPVKVSGNNDFATEE
                     IGEPNHGGYPDTSRSVWYSWTAPVSGRIRVKACSPDASFFVGVYTGNTFGTLTDTSEY
                     FYYAQCSRFFDAVAGTTYRFAVAGAPFAGTHGPFTFELRQVAIPGNDAFTSAIDLGNG
                     VSITRTGTTVDATTEDYEPNHTGGYGGGFGGSVWYRWKAPNDKPVILQACSQTEANII
                     AVYRNNPESDQTAPGNLSLLDSDSTDCLGGTKGGRLAIAPVKGTLYFIAVVAALSDYE
                     SDFNLKIQGIPTSTKSAFNLKKAIAKCRKIKGKNAKAKRKRANCIKAARKKAAIIKCK
                     KIKNAKSRNKCIKKARKRYR"
BASE COUNT          891 a         1544 c         1692 g          948 t
ORIGIN      
        1 ccccgccatc ttcccgaatc cgccggggga gagtcctgaa ggctgatcac ttgaccgggc
       61 atgagaggca ctctcaacca gaagcaggca atcaggttgc tcgaatcaaa tgggtggacg
      121 cgtacaactg gcggtaagca tcaggtcaag atgaccaaac cggggcgacg accgatcacc
      181 cttccggata accacaggag ggattacccg accggattga ccatggcgat tctcaaacag
      241 gcggggctaa gatgagtaac tggaacatga tgacgctgac cgcacagatt caccgcgaga
      301 acggcgccta ctgggccgac attcctgatt tgccgggagt gttcgcaacc ggggacactc
      361 tcgacgaatt gttcgactct ctcgaggaag gtgtctcatc ggtcctggaa agcgaatccg
      421 gctcgggcac cgttaacgtc aatggtgccc tgatatcgat cagctgaaaa tcgaaaaggc
      481 agtttggagc gatgccgggc cagcgcgttc tacgaaggat cagcgggtgg cggggatgac
      541 ggcgatctgt tcgaggtcga acttgccgtc ggcctcgtcg ggatcgtcgc cgagcgggta
      601 gttgccggtg aagcaggcgt cgcagtggac ttcggccgga gtgccgaccg cttcgtagac
      661 gccttccatc gagaggtagg cgagcgagtc ggcgccgatg tgttcggcga tctcctcgat
      721 cgtcctttcg tgggcgacca tctcgtcacg ggccgacatg tcgacgccgt agttgcagcc
      781 gaagcggatc ggcggagcgg agatcctcag gtgaacctcc ttggccccgg cgtcgcggag
      841 catgccgacg atctggcggg tcgtgttgcc gcggacgatc gagtcgtcga ccacgaccac
      901 gcgcttgccg gagatgatct ccggcagcgg gttgaacttc atccgcaggc cgtgctttcg
      961 gagctcctgg ccgggctgga tgaaggtccg ggccacgtaa cggttcttga tcagtccgtc
     1021 atcctgcggg aggccggaag cgcgggcgta accacgggcg gccggggtgc cggagtcggg
     1081 gaccgagatg accaggtcgg cgtcggccgg ggcttcgcgc cagaggatct cgcccatctg
     1141 cccgcggacc tcctgcaggc gcttgccttc gagcttggtg tccgggcggg agaagtagat
     1201 gtgctcgaag acacacatcg cggtgcgctc gctcttgatc acctgcctgg actcgaggcc
     1261 gcgttcgctc agcgagatca tctcgccggg gctgacctca cggaccagct tggcgccgat
     1321 gatgtcgaag gcgcagctct cggaggcgaa gacatagctg ccgtcgggaa gctggccgag
     1381 cgaaagcggg cggatcccgt agggatcgcg gaaggcgacg atcgcatgct tggtcatcac
     1441 cacggtcgag taggcgccct cgagctgcgg catcacgttg gcgaccgcat cctcgacgtg
     1501 gcggccgtag tcgctggaga tcagcgcggc gatgatctcg gagtcggtgg taccgcggaa
     1561 ttcgaggccc ttgcggcgca gccggttgta gatcccgacc gcgttggtca ggttgccgtt
     1621 gtgggcgagg gccagttcgc ggccgtcgtc gcgccagatc ggctgggcgt tctcccatga
     1681 gccgccgccg gtggtcgagt agcggacatg cccgatcgcc atgtcaccgg taagggctcg
     1741 cagcttgtcc tcatcgaaga cctgggagac caggccgccg tcacgcatgg tcgtgatgtg
     1801 gccgccctcg caggtggcga tgccggccga ctcctggccg cggtgctgga gcgcgtagag
     1861 actgaagtac gccaagcggg acacgtcgtg tccgggggcg tacacgccga agacaccgca
     1921 ctcgtcgcgc ggtccctcgc ggtcaaggtc gatccgctca gtcacaggtc aggccaccaa
     1981 ggatggaaaa ggacaaattc gcaggcggtc tgcctgcatt ggcagagtag cagcgcaccc
     2041 ccgcgagccg ccaatttcgg cgaaaaatgg ccgcaaatcg cgaggttcac atccgtcagg
     2101 cgatccgcag tagagttgcc ccgtggtcaa gtccagggga atcaggggat cgggattcat
     2161 cgccttgacg gctctgctgg ccgtggcgct gctcgccatg ccggcggatt ccggtgcggc
     2221 caccaagaag cagcggcagt gccagcgggc catcacctcg gtcttcacct tcaagaagcg
     2281 agttccgaag aggaagcgca agaaggcggt caggatcatc tgccagacca tgaccatcgg
     2341 caagaccggt ccgaagggtg cgaccggagc gaagggaacg accggaccaa tcggacccct
     2401 tgggtcaacc ggcccgatcg gcccgacggg gaccactggt gcgacagggg cgatcggcct
     2461 gccgggcgtc ccgggaccaa ccggtgccac gggaattcag ggcctgatcg gcccgaccgg
     2521 cccgatcggg ataaccggac cgacgggtgt cacaggactg atcggcgaga tcggaccgac
     2581 ggggccgacc gggctcacgg gactgatcgg tccgaccgga gccaccgggc tcacgggagc
     2641 cctgcccatg gtcttcgcgt cgattccctc gagttctccc caggtgatga acgactcggt
     2701 caacacggtg accggctgga ccgacgagta cgacccctcg ggtaccttcg atgcgtcgtc
     2761 cggaaccttc acggccccgc actccggcta ttacctgatc gagcccgaaa tcaccaccgg
     2821 gccggcctcg gcggtgaccg cgaccggtgg ccagccaccc gttctgatca ccgaagtgaa
     2881 cggccttggt gaggacattc aggacttccc cctcttcaac acggatctca ccctgctcac
     2941 catgctggtt ccgctccaga cggcacaggt gtcgggtgaa tccctccagc aactgagcag
     3001 tgacgacgag gtcgtgattc aggtcatcaa gcaggatggc atcgattacg acacttacgg
     3061 tgatctgaag atcaccgagc ttccggcccc ctagggcccg attcgcctgg aaacctgatc
     3121 tctggtccgt tctgtcttct agcggtaccg cttacgggcc ttcttgatgc atttgttccg
     3181 cgactttgcg ttcttgatct tcttgcactt gatgatcgcg gccttcttgc gggcggcctt
     3241 gatgcagttg gcgcgcttgc gcttcgcctt ggcgttcttg cccttgatct tcctgcactt
     3301 ggcgatcgcc ttcttcaggt tgaaggccga cttggtgctg gtcgggatcc cctggatctt
     3361 caggttgaag tccgactcgt aatcagacaa ggcagccact acggcaatga agtagagggt
     3421 ccccttgacg ggggcgatcg ccagccggcc gcccttggtt ccgcccaggc agtcggtcga
     3481 gtcgctgtcg agcagggaca ggttccccgg cgcggtctgg tcgctctcgg ggttgttccg
     3541 gtagacggcg atgatgttcg cctcggtctg tgagcaggcc tggaggatca ccggcttgtc
     3601 gttcggggcc ttccagcgat accagaccga gccgccgaag cccccgccgt agccgccggt
     3661 gtggttgggc tcgtagtcct cggtcgtggc gtcgacggtc gtcccggttc tcgtgatgga
     3721 aacgccgttg cccaggtcga tcgccgaggt gaaggcatcg ttgccgggga tcgccacctg
     3781 ccggagttcg aaggtgaacg ggccgtgcgt gccggcgaac ggagcccctg ccacggcgaa
     3841 ccggtaggtg gttccggcca ccgcatcaaa gaagcggctg cattgcgcgt agtagaaata
     3901 ctcgctcgtg tcggtcaggg ttccgaatgt gtttccggtg taaaccccga cgaagaagga
     3961 ggcgtcgggg ctgcaggcct tgaccctgat ccttccgctg accggagcag tccagctgta
     4021 ccagaccgac ctgctggtgt cggggtaacc cccgtggttc ggttctccga tttcctcggt
     4081 ggcgaaatcg ttgttgccag agaccttgac cggcaggttc ggcccgacta cctgggcgtt
     4141 ggcgaagttg tcgttggtcg gaggcggtgc cgatttcagg cgcaatacga atgtgaattc
     4201 gcccccgaag tggccgctca ccgcgatcct gtaagtggtt ccagcgacta ccgggaagtc
     4261 gaccgcacag tcgttggagg tgttcttggc gacttccacc agagtgccca gggttgcccc
     4321 tgtgtagatg gcagcgacct cgttcagcga tccgtcgacc agttccgcct cgcatgcttc
     4381 cagccgcacc cgctcggtgg caggcgcggt ccagcgaaac cagaccgatc gtgaattgct
     4441 tgatccaccg agcgtccccg gttcacccgc ctcccagttc gaatcgaccg tggtcccgtt
     4501 cgcctcgatc ggcaaggccg ggccgatgtt ggtcggggtg gcgaagttgt cgttggcggg
     4561 cggagtgagt ttccgcaggg tcaggtcaaa cgtgccctcc ttgtcgtacc agtcgatctg
     4621 aaagttgtag gtgacccccg cgtttgcgtg gaatctcagg gagcagggtc cgttggtctc
     4681 cttgacggag acgagggtgc ccagggtcac gccgctgcgg atggccatga ccggtgagtc
     4741 attctcgttg ccaccactga agttcgcgtg gcagaaatca agcacggcgt cggtttcggc
     4801 cggtgcgacc cacttgaacc agaccgatga caagggcggg atcgagtagg tgtccggctc
     4861 acccggttcg gcggtggcgc cgatgttgtt gccggtgacc gtgatcggca atgtgccggt
     4921 cagcgcctgg gcgttcgcga aattgtcatt ggccggcgcg gcgagcgcct ggccggcagg
     4981 cccgagcagc atcaacgatg cggcgatcgg gatgagacga agaaagaaac gaattctgta
     5041 attcacgacg gctgccctcc ggcctagcgg aaccc
//
LOCUS       DAOTIP010000045         5018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_19847_length_5018_cov_3.538586, whole genome shotgun sequence.
ACCESSION   DAOTIP010000045 DAOTIP010000000
VERSION     DAOTIP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19847_length_5018_cov_3.538586"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="P5138_01495"
     CDS             complement(<1..722)
                     /locus_tag="P5138_01495"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HRV59281.1"
                     /translation="MSVFRNLEARIEGLVEGVFSRAFSSEVQPVELARKLAKEMDSHK
                     TASVSRVYVPNEYTVHLSEKDRDKLEGYERSLEQELSGYLLEHARRKGYDLLTRPEVH
                     FNTDSRLRLGEFGIQARLVKPPAREGEMPRQADEGHTMVYKVAPQPPEPTEKAPDRPA
                     LTRAVVDLNDRRYVLDGPRAVLGRSDDAECVLRDPNVSRRHAELRQDASGQWEIFDLG
                     STNGVKVNGRRVTEAPLREGDQV"
     gene            complement(756..2309)
                     /locus_tag="P5138_01500"
     CDS             complement(756..2309)
                     /locus_tag="P5138_01500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRV59282.1"
                     /translation="MNGTIVLNRFEMGDRLGAGGFGTVYRAWDRRLEREVAVKVIETA
                     AESGPRIQREAKAAARLNHTGIVTLYEFAHHEYGREGGRAYLVSELVDGETVRELIDR
                     DLMSDHEIAEIGIDICEALDHAHSREVIHRDIKPANLIVPNGRGGAKLMDFGVARLTD
                     GDDLTNTGDVLGTLAYMSPEAAEGNPVTPAGDVYSLALTLFEAWTGENPRRKPTPSLT
                     LRAIERDLPLLADLRPDLPPGMTEVVDACLDREPGFRPVIEDLGLALEEALPELDRTV
                     PGDDRKISGLTSFADGGLDLGRIACAAAVCGMVATGMIVSGQADAASVGVLTIVSALL
                     TLLRPKAGFLAGGLLLAVWLVVIASMPGAALVVLLVSVPPALLIRGSGSPLALAVLGP
                     LLGTLGMAPFLPLLAALATDWRDRAVVAVTGLFFTAFAESVTGRTLLFGKIEKAPTGW
                     EDSVSGAVTGLIVPVVSSPSFLLSVAVWAGVALLVGAIVGWTRQRSGGQVREPLTVAP
                     VGSSRVPNA"
     gene            2477..3709
                     /locus_tag="P5138_01505"
     CDS             2477..3709
                     /locus_tag="P5138_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRV59283.1"
                     /translation="MEFSNPYNPDDRENDPFADGPDEDLFVDEPTVESEPDPEPDLDD
                     AGTPPPPPRRRSGGSGGGSRRGGRGGSRGGASGGDRQQLMVRRAIALGAGLLVLILLV
                     FGAKGCLDARKNRSLEEYANSVTQIVNETNALGKSFFGRLSDPGELSVTEFTSEIQSD
                     RSAMDGFLSRVEKLDTPGDMSAAQDSLTLVYELRASAMDEIADKMSTALGEEGADRAR
                     RQIAGQMEVLSAADVLYNRVTRHQIDNTIASNGANAPAMPVSTFVPNAADWISASDID
                     DALGGVSGSTGGTPDDGLSHGTGIDPASSVTIGGTALSPDTTTTIPAGTDPTVDVTVQ
                     NQGEATENDVTVSVSVDGGNALEQSIPEIAPGSTGTASIPLTPAPTGEVTLDVKVDAV
                     PGESITTNNEASYTVSFG"
     gene            3717..4574
                     /gene="pheA"
                     /locus_tag="P5138_01510"
     CDS             3717..4574
                     /gene="pheA"
                     /locus_tag="P5138_01510"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRV59284.1"
                     /translation="MRVAYLGPAGTFSEDALEVSRFAPEDFHPMACETIPAAIEAVAG
                     GDADRALVPVENSIEGSVRPTLDSLIEQADRVRIAGEYDHEIRSALIARSGIEPDAIE
                     VVISHPQPLAQCALFLRTEMPRAEVRIASSTAAAVRDVAMNEGAWAALAPSRAARLYD
                     CEVIREGIEDEPGNVTRFLWLAPSSAEDPDGIDGTGRGGRWKTSLAFAELGADHPGAL
                     VEALLEFSDREINMVRIESRPMRRELGRYRFFIDIEGRTGEAPVDAAIEGLKSKAEWV
                     RILGSYPAG"
     gene            complement(4646..>5018)
                     /locus_tag="P5138_01515"
     CDS             complement(4646..>5018)
                     /locus_tag="P5138_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRV59285.1"
                     /translation="KLTETKGGLAGALIVREHQELMFITENGMVQRTGAGGISKTGRA
                     TQGVRVMNVKDGDRISAVALVVEAANGEAGEAAEGAPEQPELEAAEDGATAEDGATTD
                     DGATADSGEATDSGEEAADSE"
BASE COUNT          851 a         1663 c         1664 g          840 t
ORIGIN      
        1 acctggtcac cttcgcgaag cggagcctcg gtgacccggc gcccgttcac cttgacccca
       61 ttcgtcgaac cgaggtcgaa aatctcccac tggccggaag cgtcctgcct gagttctgcg
      121 tgccggcgtg aaacgttggg gtccctcagc acgcattcgg catcgtcaga gcggccgagc
      181 acggcgcggg ggccgtcaag cacgtagcgg cggtcgttca ggtccaccac cgccctggtc
      241 agggcaggcc ggtccggggc cttttccgtg ggttccggcg gctggggcgc caccttgtag
      301 accatggtgt gaccttcgtc tgcctggcgg ggcatctccc cttcccgggc gggaggcttg
      361 accaggcggg cctggatccc gaattccccg agccggagcc ggctgtcggt gttgaagtgc
      421 acctccggcc gggtcaggag gtcatagccc ttgcggcgcg catgctcgag caggtagccg
      481 gaaagttcct gttcaagcga cctttcgtag ccctcgagct tgtcacggtc cttctcggag
      541 agatgaaccg tgtattcgtt cggaacgtag accctcgaga ccgaggccgt cttgtgggaa
      601 tccatctcct tggcgagctt gcgggcgagc tcgaccggct gcacctccga cgagaaggca
      661 cggctgaaga ccccttcgac gaggccctcg atgcgggctt cgaggttgcg gaaaacactc
      721 atcgccgcga ctcagttgat ggctttttcg accatctagg cgttggggac acgacttgat
      781 cctacgggag ctacggtcag cggttcgcga acctggccgc cactgcgctg cctggtccag
      841 ccgacgatcg cgccgaccag cagcgccacc cccgcccata cggcaaccga gagcaggaac
      901 gaaggcgaac tgaccacggg gacaatcagc cccgttaccg caccggagac cgaatcctcc
      961 cagccggtcg gggccttttc gatcttgccg aagagcaggg ttcgaccggt cacggactcg
     1021 gcgaaagcag tgaagaagag accggtgacg gcgaccacgg cccggtcccg ccagtcggtg
     1081 gccaaagcgg ccagcagggg caggaatggc gccatgccca gggttccgag cagcggaccc
     1141 agcaccgcaa gggccagcgg gctgcccgac ccccggatca gcagggccgg gggcacggaa
     1201 accagaagga cgaccagggc ggcaccgggc atcgaggcga tcaccaccag ccagacggca
     1261 agcagcagcc cgccggccag gaacccggcc ttgggccgca gcagggtcag cagcgcggaa
     1321 acgatggtga ggacgccaac cgaggcggca tcggcctggc cgctgacgat catgccggtc
     1381 gcgaccatgc cgcagaccgc tgcggcgcag gcaatccggc caaggtcgag gccgccgtcg
     1441 gcaaaggaag tgagcccgga gattttccgg tcgtcgccgg gaaccgtccg atcgagttcg
     1501 ggcagggcct cctcgagggc gaggccgagg tcctcgatca ccggccggaa accgggttcg
     1561 cggtcgagac aggcgtcgac cacctcggtc atgcccgggg gaaggtcggg tcgcaggtcg
     1621 gcgagcagcg gcaggtcacg ttcgattgct cgcagggtga gactcggggt gggcttgcgc
     1681 ctggggttct ccccggtcca ggcctcgaag agggtgaggg ccagcgaata gacgtcgccg
     1741 gcgggggtaa ccggatttcc ctcggccgct tcgggcgaca tgtaggcaag ggtcccgagg
     1801 acatcaccgg tattggtcag gtcgtccccg tcggtcagcc gggccacccc gaagtccatc
     1861 agcttggctc caccgcggcc attcggcacg atcaggttgg cgggcttgat gtcccggtgg
     1921 atcacttcgc gggaatgggc gtgatcaagc gcctcgcaga tgtcgatccc gatctcggcg
     1981 atctcgtgat cgctcatcag gtcacggtcg atcagttcgc gcaccgtctc gccgtcgacc
     2041 agctcggata cgaggtaggc cctgccgccc tcgcggccgt attcgtggtg ggcgaactcg
     2101 taaagggtga cgatgccggt gtggttgagc cgcgccgcgg ccttcgcctc ccgctgaatg
     2161 cggggacccg attcggcagc cgtctcgatc accttgaccg cgacctccct ttcgaggcgg
     2221 cgatcccagg cccgatagac cgtgccaaac cctccggctc ccagccggtc acccatctcg
     2281 aaacggttca ggacgatggt tccgttcatc acccagcacc tttcggcatc gactggcctg
     2341 accctccccc cgcgagcccg cttgccagtg ggaaacgcga ttccgtaaaa gtccctgcaa
     2401 aatggcgtgc cggggggcga tttgcgggtg aagggaatac attgaccacg ccgaaagtcg
     2461 ttcggcaggt ggactagtgg agttctctaa cccttataac ccagacgatc gtgagaacga
     2521 tcctttcgcc gacggaccgg atgaagacct cttcgtcgat gagccgacgg tcgaatcgga
     2581 accggatccg gagccggatc tcgacgatgc cggaacgcct ccgccgcctc cgaggcgtcg
     2641 ttcgggtggg tccggcggcg gttcccgccg tggcgggcgg ggtggatcgc gcggcggcgc
     2701 ttccggaggc gaccggcagc agctgatggt ccgccgggcg attgcgctcg gtgccggact
     2761 tctggtcctg atccttctgg tcttcggtgc caagggctgc ctcgacgccc gcaagaaccg
     2821 ctccctcgag gagtatgcga acagcgtcac ccagatcgtc aacgagacga acgccctggg
     2881 caagtccttc ttcgggcgcc tcagcgaccc gggcgagctt tcggtgaccg agttcaccag
     2941 cgagatccag tcggatcgct cggcgatgga cggcttcctg agccgggtcg agaagctgga
     3001 cacgccgggt gacatgagcg ccgcacagga ttccctgacc ctcgtttacg agcttcgagc
     3061 ttcggcgatg gatgagatcg ccgacaagat gagcaccgcc ctcggtgagg aaggggctga
     3121 ccgcgcccgc cggcagatcg ccggccagat ggaggtcctg agcgccgccg acgttctcta
     3181 caaccgggtg acccgacacc agatcgacaa cacgattgcc tccaacgggg caaacgcccc
     3241 ggcaatgccg gtcagcactt tcgtcccgaa cgctgctgac tggatttccg ccagcgacat
     3301 cgacgacgcg ctcggcggag tttccggttc gaccggtggc acgccggatg acggtctttc
     3361 ccacggcacg ggcatcgacc cggcttcttc ggtgacaatc ggtggcaccg ccctgagccc
     3421 cgatacgacc acgacgatcc cggcgggaac cgaccccacg gtcgatgtga cggtccagaa
     3481 ccagggggag gcgacggaga acgacgtcac cgtctcggtt tcggtggatg gcggcaacgc
     3541 gcttgagcag tcgattccgg aaatcgctcc cggatcgacc ggcaccgctt cgatcccgct
     3601 cacccctgcc ccgaccggcg aggtgacgct ggacgtcaag gtggacgcag ttccgggcga
     3661 gtcgatcacc acgaacaacg aagcttccta caccgtcagc ttcgggtagg cgcaccttgc
     3721 gtgtcgccta tctcggcccc gccggtacct tcagcgagga tgcgctggag gtcagccgct
     3781 ttgcgcccga ggactttcat ccgatggcct gcgaaacgat tccggccgcg atcgaggcgg
     3841 tcgccggtgg cgacgcggat cgcgcccttg tgccggtgga gaactcgatc gaggggtcgg
     3901 tcaggcccac cctcgacagc ctgatcgaac aggctgaccg ggtcaggatc gccggcgagt
     3961 acgaccacga gatccgttcg gccctgattg cccgcagcgg aatcgaaccg gatgcgatcg
     4021 aagtggtgat ttcccatccg cagccactgg cccagtgcgc gctgttcctt cgcaccgaga
     4081 tgccgcgggc ggaggtccgg attgcctcca gtaccgcggc ggcagtccgt gacgtggcga
     4141 tgaacgaagg agcctgggcg gccctggctc cctcccgggc cgcccgcctc tacgactgcg
     4201 aggtgatccg cgagggaatc gaggacgagc ccggcaatgt gaccaggttc ctttggctgg
     4261 ctcccagttc agccgaggat cccgacggca tcgacggcac cggccggggc gggcgctgga
     4321 agaccagcct cgctttcgcc gaactgggcg cagatcatcc cggtgccctg gtcgaggccc
     4381 tgctcgagtt ctccgaccgg gagatcaaca tggtccgcat cgagtcgaga ccgatgcgcc
     4441 gggaactggg gcgttaccgc ttcttcatcg acatcgaggg ccggactggt gaggcccccg
     4501 tcgacgccgc gatcgagggc ctcaagtcaa aggccgaatg ggtccggatc ctcgggtcct
     4561 accctgcggg gtgaccggaa gccaaaggtg aaactcattc ggtgccatca aggcacgaaa
     4621 tgagtttcac cccttgccaa taccgctatt cggagtcggc agcttcctcg ccgctatcgg
     4681 tggcttcacc gctgtcggcc gtggcaccgt cgtcggtcgt ggcgccatct tctgccgtag
     4741 cgccgtcttc tgcggcttcg agctcgggtt gttccggagc tccttcggct gcttcgccgg
     4801 cttctccgtt ggcagcctcg accacgaggg cgacggcgct gatccggtcg ccgtccttga
     4861 cgttcatgac cctgacgccc tgggtggcac gaccggtctt gctgatcccg ccggcaccgg
     4921 ttcgctgaac catgccgttc tcggtgatga acatcagctc ctgatgctca cggacgatca
     4981 gggcaccggc caggccaccc ttggtctcgg tcagcttg
//
LOCUS       DAOTIP010000046         4997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_19971_length_4997_cov_3.351679, whole genome shotgun sequence.
ACCESSION   DAOTIP010000046 DAOTIP010000000
VERSION     DAOTIP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19971_length_4997_cov_3.351679"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="P5138_01520"
     CDS             complement(<1..606)
                     /locus_tag="P5138_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRV59286.1"
                     /translation="MIAIAGFALSCFGLLLFVWVAFGGPTPLAARGYEVKLPLTQIGQ
                     LAEQSEVDISGVEVGRVKAIDLGEGSEDGTAIVTLNIEPEYAPIPADTRAILRAKSLL
                     GEAYVELTPGDKRDGMLEDGDQLPPAQVAKSVQLDEIFRTFDAKTREAFMQGAIDNSI
                     AINGRGATLNQTLGILTGTLTSLTDVLQILNDQEPEVSKLIR"
     gene            complement(636..1943)
                     /locus_tag="P5138_01525"
     CDS             complement(636..1943)
                     /locus_tag="P5138_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59287.1"
                     /translation="MKRAIKTHFKDFLAITGLIILALAVLLFILSNQKAALPSWIPWL
                     GQDFYSINVEMETAQAVTPGQGQAAVIAGINVGKVGASSLEDGIAHVRLDIEPEYKEL
                     IHPDAQFLLRPKTGLSDMVVEIDPGTKGPTPPEGTTFPVSQSMSNVQMDQILASLDRD
                     TQDYLVLLLNGAGEGLHNKGPELSQALRRFGPFATYTARLNGELQKRRKYIARGVHAF
                     SQVATELGNNDQVVADFVTNSRNSLANYAAQEASLREALREFPSTLDAGRVNLAKSNQ
                     LSQLTRPTLLRLIPGARHLKSSLRSVQRLAVDTTPLVRDDIRPFTRETQPVFRELAKT
                     SRASEKSTPQLQGTFTHLNTLFNELAYNPPGDSEGFLFYAPWASHNFNSSVAFQDGLG
                     PVRRSVSMLSCDTSNAAESVFGQNVQLETAYRLNQLPPPTAVC"
     gene            complement(1940..3445)
                     /locus_tag="P5138_01530"
     CDS             complement(1940..3445)
                     /locus_tag="P5138_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRV59288.1"
                     /translation="MSQDHRKPSRYNQQFFRQWGGPGPLFFGLVALAVVVIGLWLAFT
                     KSIPFTSHGYQVQATFRNAVNIAIKSPVRIAGVNVGKVTDLEQKGDNTVVTFTVDDEG
                     RPVREDAAAQIRPRLFLEGNWFIDLDPGTPESKEMPDNGMIPVSHTATSVQVGDLLRT
                     LQTPERANIQRLLSSLGTGLNAKPTAEQDLTFEPFVQGLSGGEALNLAYRQGEVAARG
                     TAIVNEATLGTQPNDLGNLLRGLSRVTGAVDERGDDLQGLVTNFATFTGALAAESENL
                     GLTIQKLGPTLVVARRSLTSLNASLPALRGFAIASTPGLNELPRTIRVTTPLLRQLQK
                     LLTKPELAGLTQILKNSTPAAARSGAATLKLLPQLRSFGLCIADTISPTGDQVIQDGS
                     FTNGQKASREFFYAAVSTTGSTNGLDGNGSFARIQGGGGPVTVKMNDDEGVTVGSKVL
                     YGRLQSAPLGTSPLAPGTPPIVKDQPCSEQEIPDLNGPMAGPGAPLPVAYP"
     gene            complement(3442..4917)
                     /locus_tag="P5138_01535"
     CDS             complement(3442..4917)
                     /locus_tag="P5138_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRV59289.1"
                     /translation="MKRRRRSRISASPVLVGAITAVIAVVAVFFSYNANKGLPFVPTY
                     EVKAELPSAASLVSGNEVRIAGVRVGIVSQVKAKQLDDGRSIAVATMKLDKSVQPIPD
                     DSTVLVRQRSAMGLKYLLLTPGKSDQGLPSGGTIPESQAEEPVDMDQWFNMFQAPVRR
                     AIQKNLAEYGGGLAARGSDINQLIQDLPPLLRVAEPVGRNLSSKQTDLRGFVRGLAQA
                     AAEVAPVAETQADMFVQLDTTFTALAEVARPYIQDSISEGVDAELSVIRNGPRIRPFL
                     YASADFMHAFRPGAKALGESAPIVARSFDIGIPVLRSSPQLYDQLAPTARSLRRFGES
                     TANNEGLDTLISTNKILTPLLRQVTPSQNVCNYANLLLRNANFTTNGGTAGGTYIRAI
                     SVLPSVGPNGEGNPASGMANGGGIPPSGGGPDKNFLHSNPYPNTAAPGQSPRECEPGN
                     PPDDWYVAGNWPAAVTIGNIPGDQGVVTAFQTQAQLDWKTK"
BASE COUNT          823 a         1499 c         1724 g          951 t
ORIGIN      
        1 cctgatcagc ttcgacacct ccggttcctg gtcgttcagg atctggagca cgtcggtcag
       61 cgaagtgagg gtgccggtca ggatgccgag agtctggttg agggtcgcgc cacgcccgtt
      121 gatcgcgatc gagttgtcga ttgcgccctg catgaaagct tccctggtct tggcatcgaa
      181 ggtgcggaaa atctcgtcga gctggaccga cttcgccacc tgggccggcg gcagttggtc
      241 accgtcttca agcatgccgt cccgcttgtc acctggggtc agctcgacgt aggcctcgcc
      301 gagcaggctc ttggcccgca gaatcgctct cgtgtcggcg ggtatcggcg cgtattcagg
      361 ctcgatgtta agcgtcacga tcgccgtgcc atcttcgctg ccttcaccga ggtcgatcgc
      421 cttgacccgt cccacttcca ccccggagat gtcgacctcc gactgctcgg ccagctggcc
      481 gatctgggtc agcggcagct tgacctcgta gccacgcgcc gccaatgggg tcgggccgcc
      541 gaaagcgacc cagacgaaaa gaagcaaacc gaaacacgag agcgcgaagc ccgcgatggc
      601 gatcatctgt ccgactgagg gggcctgttt attcatcagc agacggccgt cgggggcggt
      661 agttggttga gtcggtaagc ggtctcaagc tggacgttct gtccgaacac gctttcagcc
      721 gcgtttgagg tgtcgcagga aagcatgctc acgctgcggc gtaccggtcc gaggccatcc
      781 tggaaggcga ccgaggagtt gaagttgtgg gatgcccagg gtgcgtagaa gaggaagcct
      841 tccgagtcac cgggcgggtt gtaggcaagc tcgttgaaca gcgtgttcag gtgggtgaag
      901 gtgccctgga gctggggagt ggatttctcg ctggcccgac tggtcttcgc cagttccctg
      961 aagacgggct gggtttcccg ggtgaaaggc ctgatgtcgt ccctgaccag tggggtggtg
     1021 tcaactgcaa ggcgctgaac cgatctcaac gagctcttga ggtgacgggc gcccggaatc
     1081 agtcgcagca gggtcggtcg cgtgagctgc gagagctggt tcgacttggc caggttcacc
     1141 cggccggcgt ccagcgtcga cgggaactcg cggagggcct cgcgcaggga agcttcctgc
     1201 gcggcgtagt tggccagcga attgcgggag ttggtgacga agtctgcgac cacctggtcg
     1261 ttgttgccga gctcggtcgc aacctggctg aaggcatgga ctccccgcgc gatgtacttg
     1321 cgccgcttct ggagctcccc gttcaggcgg gccgtgtaag tcgcaaacgg gccgaaacgg
     1381 cggagcgcct gagagagctc cggccccttg ttgtggagcc cctcccccgc tccgttcagg
     1441 agcaggacca gatagtcctg ggtgtcgcgg tcaagtgacg cgagaatctg gtccatctgg
     1501 acgttggaca tgctctggga gaccgggaaa gtggttcctt ccggcggcgt cggacccttg
     1561 gtgccggggt cgatctcgac gaccatgtcg ctgaggccgg tcttgggcct gagcaggaac
     1621 tgggcgtcgg ggtggatcag ttccttgtat tcgggctcga tgtccagccg gacgtgggcg
     1681 atcccgtcct cgagtgagga ggcaccgacc ttgccgacgt tgatgccggc gatcacggcc
     1741 gcctgtccct gccccggagt gaccgcttgg gcggtctcca tctcgacgtt gatcgagtag
     1801 aagtcctgcc caagccacgg gatccatgac ggaagagccg ccttctggtt ggaaaggatg
     1861 aagagcagca ccgcgagggc cagaatgatc aggcccgtga tggcgaggaa atccttgaaa
     1921 tgggtcttta tcgcgcgttt cacgggtagg ccaccggtag gggtgctccc gggccggcca
     1981 tcggcccgtt gaggtcgggt atttcctgct cgctgcaggg ctggtccttc acgataggcg
     2041 gagtgcccgg cgccagcgga ctggtaccca gaggcgccga ctggagtcgg ccgtagagca
     2101 ccttcgaacc gaccgtgaca ccttcgtcgt cattcatctt cacggtcacg ggaccaccgc
     2161 cgccctggat ccgggcgaag gagccgttgc catcgaggcc attggtgctt cccgtggtgc
     2221 tgaccgcggc gtagaagaac tcgcgcgatg ccttctggcc gttggtgaac gagccgtcct
     2281 ggatcacctg gtcgccggtc ggactgatgg tgtcggctat gcagagaccg aagctgcgga
     2341 gctgcggaag cagcttcagg gtggcagcac cggacctcgc ggccgccggg gtcgagttct
     2401 tgaggatctg ggtaagtcct gccagctcgg gcttggtcag cagtttctgc agctgacgga
     2461 gcaggggcgt ggtaactctg atcgtgcgcg gcagctcgtt cagaccgggg gtcgaggcaa
     2521 ttgcaaagcc gcgcagggcc ggcagactgg cattgaggct ggtcagcgac cggcgcgcca
     2581 cgaccagggt cggtcccagc ttctggatgg tcagccctag gttctcggac tcggccgcga
     2641 gagcgccggt gaaggtagcg aagttggtta ccaatccctg gagatcgtcg ccgcgctcgt
     2701 cgaccgcccc ggtcacccgt gagaggccgc gcagcaggtt gccaaggtcg ttcggctggg
     2761 tgccgagggt ggcttcgttc acgatcgccg tcccgcgggc ggcgacttcg ccctgacggt
     2821 aggcgaggtt cagggcctca ccaccgctca ggccctggac gaagggctcg aaggtcagat
     2881 cctgttcagc ggtcggcttg gcgttgaggc cggtgccgag gctcgacagc aaccgctgga
     2941 tattggcccg ctcgggggtc tgcagggttc tcaggaggtc gccgacctgg acgctggtgg
     3001 ccgtgtgact gaccgggatc atgccgttgt ccggcatctc cttcgactcc ggtgttcccg
     3061 gatcaagatc gatgaaccag ttgccctcaa ggaagaggcg cggccggatc tgggccgcgg
     3121 catcctcgcg caccgggcgg ccttcgtcgt cgacagtgaa ggtgaccacg gtgttgtcac
     3181 ctttctgctc gaggtcggtg actttgccga cgttgacacc ggcgatgcgc accggcgact
     3241 tgatcgcgat gttcaccgcg ttgcggaagg tcgcctgaac ctggtaaccg tggcttgtga
     3301 acgggatcga cttggtgaag gcgagccaga gaccgatcac cacgaccgcc agggcgacca
     3361 gtccgaagaa cagcggcccc ggtccgcccc actgtcggaa gaactgctgg ttgtagcggc
     3421 tgggtttacg gtggtcctgg ctcacttggt cttccaatca agctgggcct gggtctggaa
     3481 tgcagtcacc actccctggt cgcccgggat gttcccgatc gtcacagcgg caggccaatt
     3541 gcccgcgaca taccagtcgt ccggcggatt gcccggttcg cattcacgcg gagactggcc
     3601 gggtgcggcc gtgttcgggt acggattcga atgcaggaag ttcttgtcgg gtccgccgcc
     3661 cgatggcggg atcccgcccc cgttggccat cccggaggcc gggttgcctt caccgttcgg
     3721 accgaccgac ggaagcacgc tgatcgcccg gatgtaggtg ccgccggccg taccaccgtt
     3781 cgtggtgaag ttggcgttgc ggagcagcag gttcgcgtaa ttgcagacgt tctgcgatgg
     3841 cgtcacctgc cgcaggagcg gggtcaggat cttgttggtg ctgatcaggg tgtcgaggcc
     3901 ctcgttgttg gcggtactct cgccgaagcg cctgagcgaa cgggccgtcg gggcaagctg
     3961 gtcgtagagc tggggcgacg agcgcagcac ggggatgccg atgtcaaacg accgggccac
     4021 gatcggagcg ctttcaccca gcgccttggc gcccggcctg aaggcgtgca tgaagtcggc
     4081 tgacgcgtac aggaacggcc tgatccgggg tccgttgcgg atcaccgaga gttccgcgtc
     4141 gacgccctcg ctgatcgagt cctggatgta gggccttgcg acctcggcca gggcagtgaa
     4201 ggtggtgtcc agctggacga acatgtcggc ctgggtttcg gcgaccgggg ccacttcggc
     4261 agctgcctgc gccagacccc ggacaaaacc gcgaaggtcc gtctgcttcg aggagaggtt
     4321 tctgcccacc ggctcggcca ccctgaggag aggtggcaga tcctggatca gctgattgat
     4381 gtccgaaccc ctggcggcaa gcccgccacc gtattcggcg aggtttttct gaatcgcccg
     4441 gcggaccgga gcctggaaca tgttgaacca ctgatccatg tccaccggct cctcggcctg
     4501 cgattccgga attgtgcccc ctgaggggag gccctggtcc gacttgccgg gagtgagcag
     4561 cagatacttg agacccatcg ccgagcgctg gcggaccagc actgtcgaat cgtcggggat
     4621 cggctgcacg ctcttgtcca gcttcatggt ggcgactgcg atcgagcgac catcatcgag
     4681 ctgtttggcc ttgacctgac tgacgatgcc gactcttacg ccggcaatcc tgacctcgtt
     4741 gcctgagacc agcgaagcgg cggaaggcaa ctctgccttc acctcatagg tgggcacgaa
     4801 gggcagaccc ttgttggcgt tgtacgagaa gaagacggcg accaccgcga tcacagcggt
     4861 gatcgccccg accagaaccg ggctcgccga gattcttgag cgacgacgac gcttcatggc
     4921 cccagcaggt agtcgaggat tttcatgcgg ctcgaggtcg aggaggcccc ggtcgcaccc
     4981 gtggtaccgc ggcttcg
//
LOCUS       DAOTIP010000047         4921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20392_length_4921_cov_2.994451, whole genome shotgun sequence.
ACCESSION   DAOTIP010000047 DAOTIP010000000
VERSION     DAOTIP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20392_length_4921_cov_2.994451"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="P5138_01540"
     CDS             complement(<1..684)
                     /locus_tag="P5138_01540"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935676.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRV59290.1"
                     /translation="MSETTQVTAPTKNARLLAWVDEIAELTQPDQIHWCDGSAEEYNA
                     LAEGLVEAGTFEKLSDAKRPNSYLALSDPGDVARVEDRTFICSEKEEDAGPTNNWRAP
                     DEMRATLKPFFEGSMKGRTMYVVPFSMGPLGSNIAHIGVQLTDSAYVATSMRIMTRMG
                     QGALDVLGDEGEFVPCVHSVGMPLEEGQEDVAWPCNAENKYIVHYPETREIWSYGSGY
                     GGNALLGKKC"
     gene            complement(823..1137)
                     /locus_tag="P5138_01545"
     CDS             complement(823..1137)
                     /locus_tag="P5138_01545"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HRV59291.1"
                     /translation="MSARGKKWPPLMALVVLLGVVCLTGCGSGDVIDDQKTEIAVRFD
                     VQEATGTKVRSVTCPDEVPVSVGTRFTCHVVARSGDDAVAELEVTSDNGDLRVLSLKA
                     AD"
     gene            1224..2438
                     /locus_tag="P5138_01550"
     CDS             1224..2438
                     /locus_tag="P5138_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRV59292.1"
                     /translation="MIFYEYEARRIVELAGIPVTKYGFCQTPEEARKIAEEIGGPTVI
                     KSQVLTGGRMKAGGVKFADTPEEAAELAAEILELEINGLMPVGVLVDPKAEVKQEYYA
                     SVVWDGNAKKPLMLFSDMGGIDIEQVAEEHPDHVGRGHVSNLMPVGDYEAKQVIAATG
                     VTGSQLTRATPILAKLARLFRENDMILAEINPLAELTDGSFVALDAHLELEAEAEGRQ
                     KKLLQQELGIPAGEIRDHYVPSEFEKNVKHLDAADHRGVIQGKDHGFEGNLGLVIGAG
                     GGSLTLTDAVRSQGGKPANYSEIGGNPSVAKACGLAKEVLKKDGVEKIAVMMSIVSNT
                     RVDIVARGVIKACLELGKDPGETIAIFRIPGAWEDEGNKILDHYGVEYCDRTVSLWEA
                     AGRAVAKIQGAS"
     gene            2435..3346
                     /locus_tag="P5138_01555"
     CDS             2435..3346
                     /locus_tag="P5138_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRV59293.1"
                     /translation="MSVFLDKDTTFIVQGITGREAINLTKECLDYGSRVVGGVTPGRK
                     GRKVHGVPVFDTVAQAVENHGGEIDGSIVTVPPGFTKDAVIEAIDNGIKLLVVVTERI
                     PRGDVAEMVEFAEMNDARIIGPNCLGLIVPEVCKMGGIGGPAKDAAKSYKPGVVGVMS
                     RSGGMTTEISSSLSAAGLGVSTAISIGGDPIIGSSYAELMPYFEADEQTKAIVIYSEP
                     GGRMEAQLADWQKEHDSRLPIVAFMAGKFMDDESMKGMNFGHAGTIVEGKEDSAAEKI
                     KRLEAAGIRVVERIDEIPDVVKERISA"
     gene            3343..3579
                     /locus_tag="P5138_01560"
     CDS             3343..3579
                     /locus_tag="P5138_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59294.1"
                     /translation="MSAETMDSVFIDVELDESAASDPELAKKLEEVCPVGIFKATESG
                     TEIVAGQLDECVLCNLCVDAAPEGSLRIVKKYES"
     gene            3740..>4921
                     /locus_tag="P5138_01565"
     CDS             3740..>4921
                     /locus_tag="P5138_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRV59295.1"
                     /translation="MNFVDDVIQKFPFSQEAVIAIDGEGKREVFCFSHLFARSLGLSG
                     AMLDRGVRRGDVVMIMVSGRIEWVVSMLACFRMGAIALPLNPQLSKEDLAYRVTAANP
                     SLAIGESDYLGNLPDGIPTFDMADLEQIFDEDIAQATPAKVADLDPEDPALIVFTSGT
                     TGKPKGIVHSQRYLLGQAIQAQHWLGAEPGDLCWCTAAPGWSKSSRNSFIAPWLRGAK
                     ALLVQGRFDPATRLDIVRQQEVNVLCQAPTEYRILARRTELGPTPSLRRMVSAGEALN
                     PEVIDDFRLATGLEIADGYGQTETGAVTGFRPGESVLRREGSMGRPLPGIETRVVAGE
                     LQLKVDTSPTFFHSYVDGRRFEGEWWPTGDLVTEDEDGYLFHEGRNDDMISSSGYRIG
                     PV"
BASE COUNT          934 a         1556 c         1584 g          847 t
ORIGIN      
        1 gcacttcttg ccgagcaggg cgttgccgcc gtagccggag ccgtaggacc agatttcgcg
       61 ggtttcgggg tagtggacga tgtacttgtt ctcggcgttg cagggccagg caacgtcttc
      121 ctgaccctcc tccagaggca tgccgaccga gtggacgcag gggacgaatt ccccttcgtc
      181 accgagcacg tcgagcgcgc cctggcccat gcgggtcatg atccgcatcg aggtcgcgac
      241 gtaggccgag tcggtcagct ggacgccgat gtgggcgatg ttcgagccga gcgggcccat
      301 cgagaagggc accacgtaca tggtgcgtcc tttcatgctg ccctcgaaga agggcttcag
      361 ggtcgccctc atctcgtccg gcgcccgcca gttgttggtc gggccggcgt cctcttcctt
      421 ttcggagcag atgaaagtgc ggtcttcaac ccgggcgacg tcgccggggt cggaaagggc
      481 cagatatgag ttcggacgct tggcgtcgga aagcttctcg aaggttcctg cttccaccag
      541 gccctcggcg agggcgttgt actcctcggc ggatccgtcg caccagtgaa tctgatcggg
      601 ctgggtcaac tcggcgatct cgtccaccca ggcgagcagc ctggcgttct tggtcggggc
      661 ggtcacctgc gtcgtctcac tcactggtgt ttttcaactc cttggtctac gcgccggcat
      721 agggaaatcg gcgcggttgt aagccggtca gaagattgtg acggagatct ggccggattg
      781 ctgtctgccc cgcattcctc tgcgggttac tactgagacc cgctaatcgg ccgctttgag
      841 gctcagcacg cgaaggtcgc cgttatcgga ggtgacctcg agctccgcga ccgcatcgtc
      901 cccggacctg gccaccacat gacaggtgaa ccgcgtcccc accgaaaccg ggacctcgtc
      961 gggacaggta accgacctga ccttggtccc ggtcgcttcc tggacgtcga accggaccgc
     1021 gatctcggtc ttctggtcgt cgatcacatc gcctgacccg cagcccgtga ggcaaaccac
     1081 gccgagcagg acgacaagcg ccatcagcgg gggccatttc ttgccgcggg cagacacgag
     1141 ggccacccta catagcggca gcgattccta gcgactcaat cggaataacc ggtcccacgg
     1201 ttctggaata gattttcggc gcaatgatct tctatgaata cgaggcaaga aggatcgtcg
     1261 agctggccgg cataccggtc accaagtacg gcttctgcca aacccctgaa gaggcacgca
     1321 agatcgccga agaaatcggc ggtccgaccg tgatcaagtc ccaggtactc accggtggcc
     1381 gcatgaaggc gggcggcgtc aagttcgccg acaccccgga agaggccgcc gagctcgccg
     1441 ccgagatcct cgaactcgag atcaacggcc tcatgccggt cggcgtactg gtcgacccca
     1501 aggccgaggt caagcaggag tactacgcct cggtggtctg ggacggcaac gccaagaagc
     1561 cgctgatgct tttcagcgac atgggcggga tcgacatcga gcaggttgcc gaggagcatc
     1621 cggaccacgt cggccggggg catgtctcga acctgatgcc ggtcggtgac tacgaggcca
     1681 aacaggtgat cgcggcgacc ggagtgaccg gaagccagtt gactcgcgct acgccgatcc
     1741 tcgcgaagct ggcccggctg ttccgcgaga acgacatgat cctcgccgag atcaacccgc
     1801 tggccgagct caccgacggt tccttcgtcg ctctcgatgc ccacctggaa ctcgaagccg
     1861 aagccgaggg gcgtcagaag aaactgctgc agcaggaact gggtattccc gccggcgaaa
     1921 ttcgcgacca ttacgttccg tcagagttcg agaagaacgt caagcacctc gatgccgccg
     1981 accaccgcgg agtcatccag ggcaaggatc acggcttcga gggcaacctc gggctggtga
     2041 tcggggccgg cggaggttcg ctgaccctga ccgacgcggt ccgcagccag ggcggcaagc
     2101 cggccaacta ttcggagatc ggcggcaacc cgtcggtcgc caaggcctgc ggcctggcca
     2161 aggaagtcct gaagaaggac ggagtcgaga agatcgccgt gatgatgtcg atcgtctcca
     2221 acacccgggt agacatcgtc gcccgcggtg tgatcaaggc ctgcctcgaa ttgggcaaag
     2281 acccgggcga gacgatcgcc atcttccgta tcccgggcgc ctgggaagac gagggcaaca
     2341 agatcctcga ccactacggc gtcgagtact gcgaccggac cgtttccctc tgggaagccg
     2401 ccggacgcgc agtcgccaag atccaggggg cctcatgagc gttttcctag acaaagacac
     2461 aactttcatc gtccagggca tcaccgggcg tgaagcaatc aacctgacca aggaatgcct
     2521 cgactacggc tccagggtcg tcggaggcgt gaccccgggc cgcaagggcc ggaaggttca
     2581 cggcgtgccg gttttcgaca cagtcgcgca ggcggtcgag aaccacggcg gagagattga
     2641 cggctcgatc gtcaccgtcc cccccggatt caccaaggac gcggtcatcg aagcgatcga
     2701 caacggcatc aagctgctgg tcgtcgtcac cgagcgcatc ccgcgtggcg acgtcgccga
     2761 gatggtcgaa ttcgcggaaa tgaacgacgc ccgcatcatc gggccgaact gcctgggcct
     2821 gatcgttccc gaggtgtgca agatgggcgg aatcggcgga cccgcgaaag acgccgcgaa
     2881 gtcgtacaag ccgggagtcg tcggcgtgat gtcccgctcg ggcggcatga ccaccgagat
     2941 ctcgtcctcg ctttcggccg cgggcctggg agtctcgacc gcgatctcga tcggcggcga
     3001 tccgatcatc ggctcgagtt acgccgaact catgccttac ttcgaggcgg acgagcagac
     3061 caaggcgatc gtcatctact ccgagccggg aggccggatg gaggcgcagc tggccgactg
     3121 gcagaaggag catgactccc gtctgccgat cgtcgccttc atggccggca agttcatgga
     3181 cgacgagtcg atgaagggca tgaatttcgg gcacgccgga acgatcgtcg agggcaagga
     3241 agactcggcg gcagaaaaaa tcaagcgtct cgaggccgcc ggcatcaggg tggtcgagcg
     3301 gatcgacgag atcccggacg tggtcaagga aaggatctca gcatgagcgc cgaaacgatg
     3361 gacagcgtct tcatcgacgt cgaactggac gaaagcgccg cctcggatcc ggaactggca
     3421 aagaagctgg aagaagtctg cccggtaggc atcttcaagg cgaccgaaag cgggacggag
     3481 atcgtggcgg gacagctcga cgaatgtgtc ctctgcaatc tctgcgtgga cgccgctccc
     3541 gagggttcgc tccggatcgt caagaagtac gagtcgtagc tctgggttag aacggggtcg
     3601 ggcaggacac ccctcctcgg cccgaaacgt cgtcggggcg cactgccgac cccgttcgcc
     3661 cgggctcctc tctggtttgg ggcttttcac cgctttttca ccaccttcac ttgggtccgg
     3721 cggctagact cggctcgcct tgaatttcgt cgatgacgta atccagaagt tccccttctc
     3781 gcaggaggcc gtcattgcca tcgacggcga agggaaacgg gaagtcttct gcttctcgca
     3841 cctcttcgcc cgcagtctcg gcctctccgg ggcgatgctg gatcggggcg ttcgccgcgg
     3901 cgacgtggtc atgatcatgg tcagcggcag gatcgaatgg gtggtctcga tgctggcctg
     3961 tttccggatg ggcgcgatcg ccctgccgct caatccgcaa ctcagtaaag aggatctcgc
     4021 ttaccgcgtc acggcggcga atccgtccct ggcgatcggc gagtcggact acctgggcaa
     4081 cctgccggat ggcattccca cattcgacat ggccgacctc gagcagatct tcgacgaaga
     4141 catcgcacag gcgaccccgg caaaggtggc ggatctcgat ccggaggatc cggccctgat
     4201 cgtcttcacc tccggcacga ccggcaagcc gaaaggcatc gtccattcgc agcgctacct
     4261 gctcggacag gcgatccagg cccagcactg gctgggtgcc gagcccggcg acctctgctg
     4321 gtgcaccgcg gcccccggct ggtcgaaatc gtctcgcaat tcgttcatcg cgccctggct
     4381 ccggggggcg aaggcactgc tcgtgcaggg caggttcgac cccgcgacga ggctggacat
     4441 cgtccggcag caggaagtca acgtgctctg ccaggcgccg accgaatacc ggatcctcgc
     4501 caggcggacc gaactcggtc ctaccccttc gctgcgccgg atggtctccg ccggcgaggc
     4561 cctgaacccc gaagtgatcg acgacttccg cctcgcgacc gggctggaaa tagctgacgg
     4621 ctacggccag accgagaccg gagcggtgac gggcttccgc cccggcgaat cggtcctgcg
     4681 ccgggaaggc tcaatgggcc gaccgcttcc cggtatcgag accagggtgg tcgcgggtga
     4741 gctgcagttg aaggtcgata cctcccccac tttcttccac agctatgtcg acgggcggcg
     4801 attcgagggt gaatggtggc cgaccgggga tctggtcacc gaggacgaag acggttacct
     4861 cttccacgaa ggccgcaacg acgacatgat ctcttcatcc ggctaccgga tcggcccggt
     4921 c
//
LOCUS       DAOTIP010000048         4896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20536_length_4896_cov_3.189424, whole genome shotgun sequence.
ACCESSION   DAOTIP010000048 DAOTIP010000000
VERSION     DAOTIP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20536_length_4896_cov_3.189424"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2642)
                     /locus_tag="P5138_01570"
     CDS             complement(96..2642)
                     /locus_tag="P5138_01570"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRV59296.1"
                     /translation="MDSGARKKLEETTRWQPGETEARIFNEWLEGGYFHPEATGTADE
                     NFSVAIPPPNVTGVLHMGHALNGSMQDALVRMNRMGGKNTLWILGMDHAGIATQTVVE
                     KRLKSEGISRHEIGREAFTERVWEWKEEFGGSIRQQYQRLGASVDYERERFTLDDGYA
                     RAVRKVFTSLYEKGYIYRDHYMVNWDPGSQSAISDLEVVNEEMTDTLFSIDYPVEDSD
                     RVLTVATVRPETMMADTAVAVNPNDERYGDLIGQYCVLPLVGRRLPIIADDHVDIEFG
                     TGALKITPGHDVNDFEIGRRHGLEEISVIGEDGRMTAEAGERFEGMTVAEAQVEVVAA
                     LKEEGVLGGEEEYVHSVPFSERSGERIEPLISLQWFCRMDELAAPAIEAVEDGRVNIS
                     PGQWKGVYLNWMREIRPWCVSRQLWWGHRIPVWYRGDETYVGEEEPEGEGWVQDDDVL
                     DTWFSSAIWPFATLGWPDDTAELKAFYPTDFLTTAREILFLWVARMIMTGLEFAGDVP
                     FHDVYVHSVIQARDGRRMSKSLGTGIDPLEEIEEHGADALRFGLLAMSSTQDVRYSDP
                     KVQQGRDLATKLWNASRLILLNTEAPEDRTVPVDPIALEDRWILSRLESTIGDYTRLI
                     NEYDFAHAAQELYSFVFSDLCDWYLEIAKPRIYDGDPEIAQVLLHTLERTLALAHPVM
                     PFVTEAIWEYHPYREGHLVVHQFPDSDSSRISEAVEAEVGEAIAVTRQLRGWRDMAGV
                     PPKIVLDAAGDPASVPEFVSRLGRVNIGPGEGEAVATVSGFGIQASDELDMEAVRSRI
                     DARRGKVEAELKKIEGKLGNERFVENAPAEVVAEEREKAERLRAELAELG"
     gene            2764..4824
                     /locus_tag="P5138_01575"
     CDS             2764..4824
                     /locus_tag="P5138_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRV59297.1"
                     /translation="MEGLNQPDAHRHPWRLTARIVTVVLIDAAALLLIEAILPGFDMH
                     GHLAALPTALGVGLVNALIWPILSRFTLKLSVLTLGLWGLFLNALLIGLALMAMPWVK
                     IAGLPEAIVISFGMAILTSLFSSLFAIDEDSTWYYNVVRAQLKRRGQVIQTDVPGIVF
                     LEIDGLAHDVLRRAMTNGNAPAMAAWVRDGSHRLEGWETDWSSQTGACQAGILHGNNH
                     EMPAFRWWEKETGRALVTNHPKDAAEMEARQSDGHGLLAEDGASRANILSGDAPHSML
                     TMSTVMQRRGKIGQDYAAYFARPYGVIKTFIHTVTEIARERRDNRRQEKTGIEPKIER
                     SRVYAIMRAWATVIQLDLQISGVVGDILAGRPSIYTTFLAYDEVAHHSGIERPETLYT
                     LSKVDREIDRIRRVVKEAPRPYHLVVLSDHGQSQGATFLQRYGVTLEEVVEKATGKEA
                     IGAGEEGEQNPRAYITAGTEEASHEDSAVGSAAKAVAKRDPNRDDLGLEEGSENDEIP
                     ELSVMASGCLGLVTFPREPGRLKLERIEELYPGLIDSLRNHPGIGFVMVDSEHGGVAM
                     GANGSNFFEINDIEGVDPLAAFGPNARDHVLRTHRFSNCPDIMINSTWWQDMDEVAAF
                     EELVGSHGGMGGTQSHPFVLHPVDLPWPDEPVIGAEEVHHILKSWLPDGSANGADARA
                     TLPA"
BASE COUNT          853 a         1637 c         1541 g          865 t
ORIGIN      
        1 ccattcgctc cagaccgagt ttccagccga tcggctcgag cgagttgaga aaggcttcgg
       61 cgtcgaaggc gtctcccgcg ccgttcattg ccgcctcagc cgagttcggc caattcggct
      121 ctgagccgtt cggccttctc gcgttcctcg gccaccactt cggccggggc gttctcgacg
      181 aaacgctcgt tgccgagctt cccctcgatc ttcttcagtt cggcctcgac cttgccgcgc
      241 cgcgcgtcga ttcgtgacct gacggcttcc atgtcgagct cgtcagatgc ctgaatcccg
      301 aagccggaga ccgttgcgac ggcttcgcct tcgcccgggc cgatgttcac gcgacctagt
      361 cgcgaaacga attccggaac cgaggcgggg tcaccggcag catcgagcac gatcttcgga
      421 ggtactccgg ccatgtccct ccagccccga agctgccggg taaccgcaat cgcttcgccg
      481 acttcggcct cgaccgcctc ggagatccgc gacgaatcgg aatcggggaa ctgatgaacg
      541 accaggtgac cttcacggta ggggtggtac tcccagatcg cctcggtgac gaagggcatc
      601 accggatggg caagcgccag ggtgcgctcg agcgtgtgga ggaggacctg ggcgatctcg
      661 ggatcaccgt catagatccg cggcttggcg atctccaggt accagtcgca gagatcggag
      721 aagacgaacg agtagagctc ctgggctgcg tgggcgaagt cgtattcgtt gatcagccgg
      781 gtgtaatccc cgatcgtcga ttcgagcctg gagagaatcc agcggtcctc gagggcgatc
      841 gggtcgaccg ggacggtccg gtcctccggt gcttcggtat tcagcaggat cagccgggag
      901 gcattccaga gcttggtcgc aaggtcgcgg ccctgctgga ccttcggatc ggagtaacgc
      961 acgtcctggg tggaggacat ggccagcagg ccgaagcgga gcgcgtcggc gccgtgctct
     1021 tcgatctcct ccagcgggtc gatacccgtg cccaggctct tcgacattcg ccgaccatca
     1081 cgggcctgga tcaccgagtg gacgtagacg tcgtggaacg gcacgtcgcc ggcaaactcg
     1141 aggccggtca tgatcatccg tgccacccaa aggaagagaa tttcccgagc cgtggtcagg
     1201 aaatcggtcg ggtagaaggc cttcagctcg gccgtgtcat cgggccagcc aagcgtcgcg
     1261 aaaggccaga tcgccgaaga aaaccaggtg tcgaggacgt cgtcatcctg cacccagcct
     1321 tcgccttctg gttcttcctc gcccacgtag gtctcgtcgc cgcggtacca gaccgggatc
     1381 cggtggcccc accagagctg gcgggagacg caccagggcc ggatctcgcg catccagttc
     1441 aggtagacgc ccttccactg cccgggcgag atgttgaccc ggccgtcctc gaccgcctcg
     1501 atcgccgggg cggcgagttc gtccatgcgg cagaaccact ggagggagat cagcggctcg
     1561 atccgctcgc cggaacgctc cgagaaggga accgagtgga cgtattcctc ctccccgccc
     1621 aggacccctt cttccttcag ggcggcgacc acttcgacct gggcctcggc caccgtcatg
     1681 ccttcgaagc gctcgcccgc ctcggcggtc atgcggccgt cctcgccgat caccgaaatc
     1741 tcttccagtc cgtggcggcg gccgatctcg aagtcgttga cgtcgtgccc cggggtgatc
     1801 ttgagggcgc cggtgccgaa ctcgatgtcg acgtgatcgt cggcgatgat cggcaggcgg
     1861 cgtccgacca gcggcaggac gcagtactgg ccgatcaggt cgccgtagcg ctcgtcgttc
     1921 gggttcacgg cgaccgcggt gtcggccatc atcgtttccg gcctgaccgt ggccacggtc
     1981 agaacccggt cgctgtcttc caccgggtag tcgatcgaga acagggtgtc ggtcatctcc
     2041 tcgtttacga cctcgaggtc ggagatcgcc gattgcgaac cagggtccca gttgaccatg
     2101 tagtggtcgc ggtagatgta gcccttctcg taaagcgagg tgaagacctt gcggaccgcg
     2161 cgggcatagc cgtcatcgag agtgaaacgt tcgcgctcgt aatcgaccga cgccccgagc
     2221 cgctggtact gctggcggat cgagccaccg aactcttcct tccattccca gacccgctcg
     2281 gtgaaggctt cccgaccgat ctcgtggcga cttattccct ccgacttcag tcgcttctca
     2341 accaccgtct gcgtggcgat gccggcgtgg tccatgccga ggatccagag cgtgttcttt
     2401 ccgcccatcc ggttcatgcg gaccagcgcg tcctgcatcg acccgttcag ggcgtgaccc
     2461 atgtggagca cgccggtcac gttgggggga gggatcgcta ccgagaagtt ctcgtcggcg
     2521 gtaccggtgg cttccggatg gaaatagccg ccctccagcc actcgttgaa tatccgcgcc
     2581 tcggtctctc ccggctgcca ccgggtggtt tcctcaagct tctttctggc tccgctatcc
     2641 aagatcagcc gatcttattg acggcactgc cccgagcgca tgtggcttcc cctgccacca
     2701 acctcggtga tcatccggat cgggtgattg atccggaccg cccggtctgg gagagttccc
     2761 ggcatggagg gactgaacca gcccgatgcg caccggcacc catggcggct gaccgctcgc
     2821 atcgtcaccg tcgtcctgat cgacgcggcc gctctgttgc tgatcgaggc aatcctgccc
     2881 ggtttcgaca tgcacggcca cctcgccgcg ctgcccaccg cgctcggtgt cggccttgtc
     2941 aacgccctga tctggcccat cctctcccgt ttcaccctga agctcagcgt cctgaccctc
     3001 ggcctgtggg gactgttcct caacgccctg ctgatcggcc tcgccctgat ggcgatgccc
     3061 tgggtgaaga tcgccgggct gcccgaggca atcgtgatct cgttcgggat ggcgatcctg
     3121 acctcgctct tctcctcgct cttcgcgatt gacgaggaca gcacctggta ctacaacgtg
     3181 gtccgggcgc agctcaagcg ccgcggccag gtgatccaga ccgatgtccc ggggatcgtc
     3241 ttcctcgaga tcgacggtct cgcgcacgat gtcctgcgcc gggcgatgac caacggcaac
     3301 gcgccggcca tggccgcctg ggtaagggat ggaagccacc ggctggaggg atgggagacc
     3361 gactggtctt ctcagaccgg cgcctgccag gccggcatcc tccacggcaa caaccacgag
     3421 atgccggcct tccgctggtg ggaaaaggag accggccggg ctctggtcac gaaccacccg
     3481 aaggacgcgg ccgagatgga agcgcggcag tccgatggtc acgggctact ggccgaagac
     3541 ggagccagcc gggccaacat cctctccggc gacgcgccgc actcgatgct gaccatgagc
     3601 accgtgatgc aacgccgggg aaagatcggc caggactatg ccgcctattt cgcccggccc
     3661 tacggcgtga tcaagacctt catccacacc gtcaccgaga ttgcccggga gcggcgcgac
     3721 aaccggcgcc aggagaagac cggcatcgag ccgaagatcg aacgctcccg cgtctacgcg
     3781 atcatgcggg cctgggccac cgtgatccag ctcgacctcc agatctcggg tgtcgtcggc
     3841 gacatcctcg ccggccgtcc ctcgatttac acgaccttcc tcgcctacga cgaggtggca
     3901 catcactccg gaatcgaacg gccggaaacc ctctacacgc tgagcaaggt ggaccgggag
     3961 atcgaccgga tccgccgtgt ggtcaaggaa gcaccgcgtc cctaccacct ggtcgtgctt
     4021 tccgaccacg gccagtcgca gggggcgacc ttcctgcagc ggtacggagt caccctggaa
     4081 gaggtggtcg agaaggcgac cggcaaggag gcgatcggcg ccggcgaaga gggcgagcag
     4141 aacccccgtg cttacatcac cgccggcacc gaagaagcca gccacgaaga cagtgcggtc
     4201 ggcagcgcgg cgaaagcggt ggcgaagcgg gatccgaacc gcgacgatct cggcctggaa
     4261 gaaggttccg aaaatgatga aatcccggag ttgtcggtga tggcgtcagg ctgcctcggt
     4321 ctcgtcacct tcccccgtga acccgggcgc ctgaagctcg aacggatcga ggagctctac
     4381 cccggcctga tcgactctct ccgtaatcac ccgggcatcg ggttcgtgat ggtcgattcg
     4441 gagcacggcg gggttgcgat gggcgcgaac ggcagcaact tcttcgagat aaacgacatc
     4501 gagggcgtcg acccgctcgc cgcattcgga ccgaacgccc gtgaccacgt gctccgcacc
     4561 caccgcttct cgaactgccc ggacatcatg atcaacagca cctggtggca ggacatggac
     4621 gaagtggccg ccttcgagga actggtcgga tcacatggcg ggatgggcgg cacccagagc
     4681 cacccgttcg tccttcatcc ggttgacctc ccctggccgg atgaaccggt gatcggcgcc
     4741 gaggaggtcc accacatcct caagagctgg ctgcccgacg gcagcgctaa cggagcggac
     4801 gcgagagcga ctctcccggc ctgagtacct gcgactccag gtccggtgcg atttccgcca
     4861 tctgctggct gaaggcggtg accggcctct caagca
//
LOCUS       DAOTIP010000049         4889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20580_length_4889_cov_3.100952, whole genome shotgun sequence.
ACCESSION   DAOTIP010000049 DAOTIP010000000
VERSION     DAOTIP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20580_length_4889_cov_3.100952"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="P5138_01580"
     CDS             complement(<1..153)
                     /locus_tag="P5138_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV59298.1"
                     /translation="MANDLYEVLGVSKKASQEEIKKAHRKLVREYHPDRNPDDKSAED
                     KFKEIQA"
     gene            complement(218..841)
                     /locus_tag="P5138_01585"
     CDS             complement(218..841)
                     /locus_tag="P5138_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRV59299.1"
                     /translation="MIQEPEETAGEPEVEEVVDAEVVSAADPAVDGAAAEEAAAEATV
                     ESDLAALVEEARSQRDEYLDLAQRTKADFDNYRKRVAAESQAQIERGRLEVISGLIEA
                     IDNLERVLDAAGIEQEVALGGSLPENAPVTEQGVIVAYRDLHAVLRKAGVEAFDSKGE
                     AFDPNLHEALQALPAEGVGSGIVLEVMQKGYRAGENVIRHARVVVSQ"
     gene            complement(838..2778)
                     /gene="dnaK"
                     /locus_tag="P5138_01590"
     CDS             complement(838..2778)
                     /gene="dnaK"
                     /locus_tag="P5138_01590"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRV59300.1"
                     /translation="MCGTGKQSNRRNKVGKTIGIDLGTTNSCVAVLEGGEPTVLENSE
                     GGRTTPSVVAFNDKDERLVGTVAKRQAVMNPENTIFSIKRFMGRKEAEVKEEETMIPY
                     KVEAGPNGDVRVDVRDKQYSPPEISAMILGKLKSDAEAKLGETVDSAVITVPAYFNDD
                     QRQATKDAGKIAGLDVKRIINEPTAASLAYGLDKEDTDQTILVFDLGGGTFDVSVLEI
                     GDGVFEVKSTAGDNHLGGDNFDKAIVDWLVAEFKKDQGVDLAADKNSLQRLYEAAEKA
                     KIELSSAQETQINIPFITAVEGQPKHLDIKLTRSKLNELTSDLLDRVVGPVKQALGDA
                     DASVDHVVLVGGMTRMPAVRDKVTELTGKEPHQGVNPDEAVAVGAAIQAGVLAGDVKD
                     VLLLDVTPLTLGIETKGGVMTKLIERNTTIPSRKSEVFSTAEDNQPSVEIHVLQGERE
                     MASYNKSLGKFQLTGIPPAPRGMPQIEVTFDIDANGIINVTAKDLGTGKEQKIEIKSG
                     SGLSDDEINSMVSDAEAHADEDKKQRELAEARNIGENAAYTSEKQLTELGDKIDGAAK
                     TEIEDRIAALRGVLESENAEDIKAKTDELQEAFHKVSEQVYQAAQAESQAAGAEGAAD
                     GAPSSDEEEVIDAEVVDEDESK"
     gene            complement(2918..4012)
                     /locus_tag="P5138_01595"
     CDS             complement(2918..4012)
                     /locus_tag="P5138_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59301.1"
                     /translation="MFALVGALAMTALVAQPADAKRKKKPNTVKVMTRNLYLGADLGP
                     AVNSSGPADFVSKNGQILRDVDTNNFPARAKGLANEIKKVSPDLVGLQELALWRTGDP
                     SLSPAISGNYVASTVHVDYLKELMKQLNKGKKRYRVVKVQNEFDFEAPADYDNDPATG
                     LATFGGEINGRLTMRDAILAKVGAGIRTRKIKASNFTTIYEPVISGITVKVQRGWVSA
                     NVKVRKGKWFRFVNTHLESFGEPTIREAQAKQLLAGPLKSGSLPAVLVDDLNSDDDTV
                     SADDALAYNALKKGGMVERGFSGKMSFGVDSGIITDDAPTKFDQFIDHVMTNRPKAVR
                     KMAAGVTGTKPANGYWDSDHLGVWAKLRMP"
     gene            complement(4178..4585)
                     /locus_tag="P5138_01600"
     CDS             complement(4178..4585)
                     /locus_tag="P5138_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRV59302.1"
                     /translation="MNKKPFVIFGLVLVLLAVVIPFWAFERDGNTANNDGVPPEDTPG
                     KQLFQTNCGNCHTLYAAGTDGDFGPNLDMKLAPAGPAADKTAVAGIKTQVLTALEEGA
                     DSPDVPGRMPAGIVTGAVAEEIANFVAHYAGEG"
     gene            complement(4632..>4889)
                     /locus_tag="P5138_01605"
     CDS             complement(4632..>4889)
                     /locus_tag="P5138_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59303.1"
                     /translation="TWNRPEIPVDVHVFRIGGRLGLFRPNASFEEAHDEMLALVDPED
                     SYEFHMNLIRHGRTLCRPKPLCRECALRRMCPYGRGPGSER"
BASE COUNT          737 a         1561 c         1529 g         1062 t
ORIGIN      
        1 agcctggatc tccttgaact tgtcctcggc ggacttgtcg tccgggttgc ggtccgggtg
       61 gtactcccgc accagcttgc ggtgggcctt ctttatctct tcctgcgatg ccttcttgct
      121 gacaccgagc acttcataaa gatcgtttgc catctatctc acctccccgt acgcctccgg
      181 ggatttcgct cgtagccctt tcaaatttct gccaggttca ctggctgacc accacacgcg
      241 cgtgacggat cacgttctca ccggcccggt agcccttctg catcacttcc agcacgatcc
      301 ccgatccgac accttccgcc ggcagcgcct gcagcgcctc gtgaaggttg gggtcaaacg
      361 cctcgccctt cgagtcgaac gcctcgactc ccgccttccg cagcaccgca tgaaggtcgc
      421 gataggcgac gatcacgccc tgttcggtga ccggagcgtt ctcgggcagc gagccgccga
      481 gcgcaacctc ctgttcgatc ccggccgcgt cgagcactcg ctcgaggttg tcgatcgcct
      541 cgatcaggcc ggagatgact tcgagccggc ccctttcgat ctgggcctgg ctttcggcgg
      601 cgacccgctt gcggtagttg tcgaagtctg ccttcgtgcg ctgggcgagg tcgagatact
      661 cgtcccgctg gctgcgggct tcttcgacca gtgccgccag gtcgctctcg accgtcgcct
      721 cggcggcagc ttcctccgcg gccgccccgt ccacggccgg atcggccgcc gacaccactt
      781 cggcgtcgac gacctcttcc acctcgggtt cgcccgcggt ctcttccggc tcctggatca
      841 cttgctttcg tcctcgtcaa ccacctcggc gtcgatcacc tcttcctcgt cagaggaggg
      901 ggcgccgtca gcagcacctt cggctccggc ggcctgggac tcggcctggg ccgcctgata
      961 gacctgctcg gacaccttgt ggaaggcctc ctggagctcg tcggtcttcg ccttgatgtc
     1021 ctcggcgttt tccgactcaa gcacaccgcg cagggccgcg atcctgtctt cgatctcggt
     1081 cttcgcggca ccgtcgatct tgtcgccgag ctcggtgagc tgcttctccg aggtataggc
     1141 ggcgttttcg ccaatgttcc tggcttcggc cagctcgcgc tgcttcttgt cctcatcggc
     1201 atgcgcttcg gcgtcggaga ccatgctgtt gatctcgtcg tccgagagtc cggagccgga
     1261 cttgatctcg atcttctgct ccttgccggt gccgagatcc ttggcggtca cattgatgat
     1321 gccgttggcg tcgatgtcga aggtgacctc gatctggggc atgccacgcg gggccggcgg
     1381 gattccggtc agctggaact tgccgaggct cttgttgtag ctcgccattt cgcgctcacc
     1441 ctgaaggacg tgaatctcga ccgaaggctg gttgtcctcg gcggtcgaga acacttcgga
     1501 cttcctcgag ggaatcgtcg tgttccgctc gatcagcttg gtcatcacgc cacccttggt
     1561 ctcgataccg agggtgagcg gggtgacgtc cagcagcagc acgtccttga cgtcgccggc
     1621 cagcacgccg gcctggatcg cagcaccgac cgcgacggcc tcgtccgggt tgacgccctg
     1681 atgcggctct ttgccggtca gctcggtcac cttgtcacgg acggcgggca tgcgggtcat
     1741 gccgccgacc agcacgacgt ggtcaaccga agcgtcggcg tcgccgagcg cctgcttgac
     1801 cggcccgacc acgcggtcga gcagatccga ggtgagctcg ttcaacttcg aacgggtcag
     1861 cttgatgtcg aggtgcttgg gctggccttc gaccgcggtg atgaagggga tgttgatctg
     1921 cgtttcctgg gcggaggaaa gctcgatctt cgccttttcg gccgcttcgt agaggcgctg
     1981 cagcgaattc ttgtcggcgg ccaggtctac tccctggtcc ttcttgaact cggcgaccag
     2041 ccagtcgacg atcgccttgt cgaagttgtc gccgccgagg tggttgtcac cggcggtgga
     2101 cttcacctca aagacgccgt cgccgatctc gagcacggac acgtcgaagg tgccgccgcc
     2161 gaggtcgaag accaggatgg tctggtcggt gtcttccttg tcgagcccgt aggccagcga
     2221 ggccgcggtc ggctcgttga tgatccgctt gacgtcaaga ccggcgatct tgccggcatc
     2281 cttggtggcc tggcgctggt cgtcgttgaa gtaggccggg accgtgatca cggccgagtc
     2341 gacggtctcg ccgagcttcg cctccgcatc ggacttgagc ttgccgagga tcatcgcgct
     2401 gatctccggg ggcgagtact gcttgtcgcg cacgtcgacg cgcacgtccc cgttcgggcc
     2461 ggcttcgacc ttgtacggga tcatcgtctc ctcttccttg acttcggcct ccttgcggcc
     2521 catgaagcgc ttgatcgaga agatcgtgtt ctccgggttc atcacggcct ggcgcttggc
     2581 aacggtgcct accagccgtt catccttgtc gttgaaggcg accaccgaag gggtggtgcg
     2641 cccgccctcg gagttctcga gcacggtcgg ttcgccgcct tcgagaaccg ctacgcacga
     2701 attcgtcgtg ccgaggtcga ttccaatcgt ctttcccact ttgtttctcc tgtttgactg
     2761 ttttccggtc ccacacaatt gcctcgcggg ccggatgctt cgatactaga aaaatctaag
     2821 tgagactgta taagattttg tacagaacgt caaattaagt agcaaaaggc ccgcaaatgc
     2881 gggccttttg ccttgctgcc aggtgttgtg actctgacta cggcatcctg agcttggccc
     2941 agactcccag gtggtcggag tcccagtagc cgttcgcagg cttggtgccg gtcacaccgg
     3001 ccgccatctt ccggactgcc ttgggccggt tggtcatcac gtgatcgatg aactggtcga
     3061 acttggtcgg tgcatcgtcg gtgatgatcc cgctgtccac gccgaagctc atcttgccgc
     3121 tgaagccgcg ctcgaccatg ccgcccttct tcagtgcgtt gtaggcgagg gcgtcatcgg
     3181 cggagacggt gtcgtcatcc gagttcaggt catcgaccag gacggccgga aggctgccgc
     3241 tcttgagcgg accggccagc agctgcttgg cctgggcttc gcggatcgtc ggctcgccaa
     3301 aggactcgag gtgggtgttc acgaaacgga accacttgcc cttgcggacc ttgacattgg
     3361 ccgaaaccca gccgcgctga accttgaccg taattcccga aatgaccggc tcgtagatcg
     3421 tcgtgaagtt actggccttg atcttccggg tgcggatacc ggccccgacc ttcgccagga
     3481 tcgcgtcccg catggtcaga cggccgttga tctcgccgcc gaaggtggca agcccggttg
     3541 ccgggtcgtt gtcgtaatcg gccggtgctt caaagtcgaa ttcgttctgg accttgacaa
     3601 cccggtagcg cttcttgccc ttgttgagct gcttcatcag ctccttgagg tagtccacat
     3661 gcaccgtgga ggcgacgtag ttgcccgaga ttgccgggga aaggctggga tcaccggtgc
     3721 gccagagcgc gagttcctgc aggccgacca ggtcggggct gaccttcttg atctcgttcg
     3781 cgagaccctt ggcccgggcc gggaagttgt tggtgtccac atcccgcagg atctggccgt
     3841 tcttgctgac gaagtcagcc ggaccgctgg aattcactgc cggaccgagg tcggcgccca
     3901 ggtaaaggtt cctggtcatc accttgaccg tgttcggctt cttctttcgc tttgcgtcgg
     3961 ccggctgggc cacaagcgcg gtcatcgcga gggcgcccac gagggcaaac accaacgcaa
     4021 ggaaggccct gtttcggccg aggggggccg gtcggcccgt catgttgaac atcaagttct
     4081 ctccttttgg tctggggcga gacgcggcaa cacgtctttt gcggttcccc aaccgctctc
     4141 aggagaataa acccggttca ggcccggaag gttcggatca gccttcgccg gcgtaatgcg
     4201 cgacgaagtt ggcgatctct tcagccaccg cgccggtgac gattccggcc ggcatgcggc
     4261 cgggcacatc cgggctgtcg gcgccctctt cgagcgcggt caggacctgg gtcttgatgc
     4321 ctgccacggc ggtcttgtca gcggccggtc cggccggagc gagcttcatg tccaggttcg
     4381 ggccgaaatc gccgtcggta cccgctgcgt aaagcgtgtg gcagttgccg cagttggtct
     4441 ggaagagctg cttgccgggt gtgtcctcgg gcgggacacc gtcgttgttg gcggtgtttc
     4501 cgtcgcgctc gaatgcccag aacgggatca ctaccgcaag caggaccagg acgagaccga
     4561 atatgacaaa gggcttcttg ttcatgtgtg gctggcgcaa aaattggacg gtgcgccgaa
     4621 cagcggggat attacctttc ggaccccggt ccgcgtccgt aggggcacat ccgccgcagg
     4681 gcacattcac ggcagagcgg cttcgggcgg cacagagtgc ggccatgccg gatcaggttc
     4741 atgtggaatt cgtaggagtc ctccgggtcg accagggcga gcatctcgtc gtgcgcttct
     4801 tcgaacgagg cgttcgggcg gaacagcccg aggcgaccgc cgatccggaa gacgtggacg
     4861 tcgaccggaa tctccggccg gttccaggt
//
LOCUS       DAOTIP010000050         4883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20612_length_4883_cov_3.349006, whole genome shotgun sequence.
ACCESSION   DAOTIP010000050 DAOTIP010000000
VERSION     DAOTIP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20612_length_4883_cov_3.349006"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="P5138_01610"
     CDS             complement(<1..2267)
                     /locus_tag="P5138_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59304.1"
                     /translation="MTDVAESGPESGPEADARFQRPSLLLGAAHLAALWTLAFVQPML
                     DLLGNNPDFFVARGNSSGQIILYALTLTFAVPLICLAIEAIVRKFNPDAQWYLHLFLM
                     GLFGAAFALQLIKNHLDWPAGLLIAISIALSLAGVFLYARWHFPQAFMDVLSVAPIIV
                     LIIFFGFSSTSRLVLPREQPDPVDVAIAKPAPVVMVIFDEFPVGSLMTPDGGIDATRY
                     PAFANLADHSTWYRNTSADGAYTPLAVPAILSGQKPNHDDLPIASDYPHSLFTLLGKS
                     YDLRVMEAATRICPDTLCPEEDNTAQDNSTSDLFSDLYVVSKYLLLPDSLTRGLPDVS
                     NSFYGFTESDQVDTNAESEVTGLDSGPTGATGITGATGPTGTTGTTGDTSPERTGQGA
                     ARKLGRLFALESSADEFERVGQFDAKLQPGQKKTLDLIHIEKPHYPWRHIPNGQRYSN
                     LTAEWSGLLPNDGPWMAPPEIVNIALQRHLLEVGYTDTLLAQIINRLKQTGLWDKALF
                     VVTADHGAAFQSKVPRRAAVTENLGEIASIPLFIKSPGQTEPKVDEQRTCATDILPIM
                     AEDLGIDYPWKKADCDPDKITVVNSPNGEGTGTPQQMLKQRQKELVNRIQSVFGTGHG
                     WGPYYRFGPHKELIGKKVKNLDVLPIKRWRALPDERNAVKNYDPTAPTLRGLLQRGVT
                     DRIPEKSVLAVAVNGRIQAVGWTFKDGTGRGPGYSILLPPESLKSGFNQVDIYQLKDD
                     GKKLRLVYDGSGRLPA"
     gene            complement(2280..4424)
                     /locus_tag="P5138_01615"
     CDS             complement(2280..4424)
                     /locus_tag="P5138_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59305.1"
                     /translation="MSDTAESAAPLGPPSIWMGGLHLLALWALAVVQPLLDLLGNNPD
                     FFVARDNTAGQIIVFVLLLTILPALLATLLEAAVDLASATARWLLHLVFCGILFSAIT
                     LQVLKHFADGPAWPMILLSLVIGGLLTWAYARAPFMRSMADILVIAPPVILVAFFFFS
                     DASELTISTPEVHAKEVTATNPAPVVMVIFDEFPAGSLMTPSDSINPRRFPHFAELRR
                     TATWYRNTATDASYTAIAVPSILTGQPADRDSLPTAADHPDSIFTLLGDNWQIHAIEP
                     ITQLCTKDICGEMSGDDVNMGEALSSLASDLKEVSAHLLLPDDMGDSLPDISQSFQGF
                     GGEEDPVQRGRARQWVHDRLSEGENSLDGEGDVATAISTLGKEGNTFDFVHVEKPHYP
                     WTHYPSGLKYSDGTEDFRGFIGDTDWLGGQYITDRAHQAHLLEVGFTDQLLGRIITAL
                     KKDGRWDDTLFVVTADHGAAWKKNAARRSVEPETMGQIGMVPLFIKSPGQKRGRTVDR
                     PACTTEIVPEMARILGIDLPWKPATCDRRTISIDNGAGAPVTVPFAKAIAQRQAYIDR
                     MARLLGGDGAGWGRAFEWGRNKDLIGRRVRRTLPAVAGRSATPETPGPENFTFDPTVE
                     LNPLLRQRGTVDGPGAGVPLAVTVNGRIAAVGQTYSDNGKTMYSILLPESSLRDGVNR
                     IAVSEVVRAAGNLSGARNQPAALRPLWSSADQ"
     gene            4564..>4883
                     /locus_tag="P5138_01620"
     CDS             4564..>4883
                     /locus_tag="P5138_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59306.1"
                     /translation="MMRRAIVALLAAACLALSAAGLLATGASAATKPSFVVIQTDDQP
                     MNEYDRRFRNLYDNWRPIMPKTMKLMRDKGITFTQYITPFPLCAPSRATLLSGRYAQN
                     HGVIR"
BASE COUNT          831 a         1490 c         1690 g          872 t
ORIGIN      
        1 gccggcaggc gcccggagcc gtcgtagacc agtcgaagct tctttccgtc gtccttcagc
       61 tggtagatgt ccacctggtt gaagccagac ttgagcgatt cgggtggcag cagaatcgaa
      121 taccccggac cccttccggt gccgtccttg aaggtccagc caaccgcctg gatacgcccg
      181 ttcacggcaa cggccaacac gctcttctcc ggtatccggt cggtgacccc ccgctggagc
      241 agtccacgca gggtcggggc ggtcgggtca tagttcttga ccgcgttgcg ctcgtcgggc
      301 agcgcccgcc aacgcttgat cggaaggacg tcgaggttct tcaccttctt gccgatcagc
      361 tccttgtgag ggccgaaccg gtagtacggc ccccagccgt gacccgttcc gaagacggac
      421 tggatccggt tgaccagctc cttctggcgc tgcttcagca tctgctgcgg ggtcccggtc
      481 ccttcaccgt tcggcgagtt caccacggtg atcttgtccg ggtcacagtc ggccttcttc
      541 cagggatagt cgatcccgag gtcttcagcc attatcggca ggatgtcggt cgcgcaggtc
      601 cgctgttcgt ccaccttcgg ctccgtctgt ccgggggact tgatgaacag cgggatcgaa
      661 gcgatctcgc cgaggttctc ggtgaccgcc gcccggcgcg ggaccttgga ctggaaggcg
      721 gcaccgtggt cggccgttac cacgaacagg gccttgtccc agagtccggt ctgcttcagc
      781 cggttgatga tctgcgccag cagcgtgtcg gtgtagccca cctccaacag atgccgctgc
      841 agggcgatgt tgacgatttc cggcggggcc atccaggggc cgtcattggg tagcagcccg
      901 gaccattcag cggtcaggtt cgagtaccgc tgaccattcg ggatgtggcg ccacgggtag
      961 tgcggcttct cgatgtggat caggtcgagc gtcttcttct ggccgggctg cagcttcgcg
     1021 tcgaactggc cgaccctttc gaattcgtcg gccgaggatt cgagtgcgaa gagccggccg
     1081 agcttgcggg ccgcgccctg cccggtccgc tccggggagg tgtcgccggt tgtcccggtt
     1141 gtcccggtcg gaccggtagc tccggtgatg ccggtagcgc ccgttggccc gctgtccaac
     1201 ccggtcactt cggactcggc gttggtgtcg acctggtcgg actcggtgaa cccgtagaag
     1261 gagttcgata cgtccggcag accccgggtc aatgagtccg gcaggagcag gtacttggag
     1321 accacataga gatcggagaa gagatcggag gtcgagttgt cctgggcggt gttgtcctct
     1381 tccgggcaga gggtgtcggg gcagatcctg gtcgcagcct ccatcactct caggtcgtag
     1441 ctcttgccga gcagggtgaa gaggctgtgc ggataatccg aggcgatcgg cagatcgtcg
     1501 tggttcggct tctggccgga gaggatcgcg ggtaccgcga gcggtgtgta ggccccgtcg
     1561 gcgctggtgt tccggtacca ggtggaatgg tccgcgaggt tcgcgaaggc ggggtacctg
     1621 gtcgcatcga tcccgccgtc gggagtcatc agcgaaccga ccgggaactc gtcgaagatc
     1681 accatcacga ccggagccgg tttggctatc gcgacgtcga ccgggtcggg ttgctcccgg
     1741 ggcagcacca gccgcgaggt ggaactgaag ccgaagaaga tgatgaggac gatgatcgga
     1801 gcgacgctga gcacgtccat gaaggcctgg gggaaatgcc agcgtgcata gaggaagact
     1861 ccggccagag acagggcgat cgagatggcg atcagcagcc cggccggcca gtcgaggtgg
     1921 ttcttgatca gctgcagggc gaaggctgct ccgaacagtc ccatcaggaa caggtggagg
     1981 taccactggg cgtccgggtt gaactttcgt acgatcgcct cgatcgcgag acagatcagc
     2041 gggacagcga aggtcagggt aagggcgtag aggatgatct ggccggaact gtttccccgg
     2101 gccacgaaga agtcggggtt gttgcccagc aggtcgagca tcggctgaac gaaggcaagg
     2161 gtccagagcg cggcgagatg ggccgctccc aggagaaggg agggccgctg gaagcgggcg
     2221 tcagcctctg gcccggactc gggcccggac tctgcgacat cagtcacgga ctggccagtc
     2281 tattggtcgg ccgaggacca gaggggcctg agagcggcgg gctgattccg ggctccactg
     2341 aggtttccgg ccgctctcac gacctcgctg acggcgatcc ggttgacgcc gtcgcgaagg
     2401 gaggactcgg gaaggaggat cgagtacatg gtcttgccgt tgtccgagta ggtctggccg
     2461 accgcggcaa tccgcccgtt tacggtcacc gcgagcggca ccccggcccc gggaccgtcc
     2521 accgtgcctc tctgtctcag caggggattc agttcaacgg tcggatcgaa ggtgaaattc
     2581 tccggtccgg gtgtctcggg ggtggcggac ctgccggcaa ccgcgggaag ggtccggcgg
     2641 acccttctgc cgatcaggtc cttgttcctg ccccactcga atgcccgccc ccagccggcg
     2701 ccgtctcccc cgagcagcct ggccatccgg tcgatgtagg cctggcgctg ggcgatcgcc
     2761 ttcgcgaagg gaacggtgac cggagcgccg gcgccgttgt cgatgctgat ggtcctgcgg
     2821 tcgcaggtgg ccggcttcca gggcaaatcg atgccaagga tccttgccat ttccggcacg
     2881 atttcggtgg tgcatgccgg ccggtccacg gtccgccctc gcttctgacc gggcgacttg
     2941 atgaagaggg gaaccatgcc gatttgcccc atggtctccg gttcgacgct ccggcgggca
     3001 gcgttcttct tccaggcggc gccgtggtcg gcggtgacca cgaagagcgt gtcatcccag
     3061 cggccgtcct tcttcagggc cgtgatgatc cggccgagca gctggtcggt gaagccgact
     3121 tcgagcaggt gagcctggtg ggcacggtcg gttatgtact gaccacccag ccagtccgtg
     3181 tccccgatga aaccgcggaa gtcctcggtg ccatccgagt acttgagccc ggaggggtag
     3241 tgggtccagg ggtaatgggg cttctcgacg tggacgaaat caaaggtgtt gccctccttg
     3301 cccaacgtgc tgatggccgt ggcgacatct ccctcgccat ccagcgaatt ctcgccctcg
     3361 ctcaggcggt catgcaccca ttggcgggcc cggccccgct gcaccgggtc ttcctctccg
     3421 ccgaagccct gaaagctctg cgagatgtcc ggcagcgagt cgcccatgtc atccggcagc
     3481 aacaggtggg ccgagacctc cttgagatcg gaggcaagcg aggagagcgc ctcgcccatg
     3541 ttgacgtcat cgccgctcat ctcgccgcag atgtccttcg tgcagagttg ggtgatcggc
     3601 tcgattgcgt ggatctgcca gttgtcgccg agcagcgtga agatggagtc gggatggtcg
     3661 gcggcggtcg gcaggctgtc acggtcggcc ggctggccgg taaggatcga aggcacggcg
     3721 atagcggtgt aggaggcatc ggtcgcggtg ttccggtacc aggtggccgt gcgccgcagt
     3781 tcggcgaagt gggggaagcg tctcgggttg atggaatccg aaggggtcat cagcgaaccg
     3841 gcggggaact cgtcgaagat caccatcacg accggtgccg ggttggtggc ggtgacctcc
     3901 ttcgcatgca cctccggggt ggagatggtc agctccgagg cgtcgctgaa aaagaagaag
     3961 gcgaccagga tcaccggcgg cgcgatgacc aggatgtcgg ccatcgagcg catgaaaggc
     4021 gcccgggcgt atgcccaggt gagcagcccc ccgatcacga gggacagaag gatcatcggc
     4081 caggcagggc cgtcggcgaa atgcttcagc acctgaaggg tgatcgccga gaagagaatc
     4141 ccgcagaaga cgaggtgaag cagccagcgg gcggtcgcgc tggccagatc cacggcggcc
     4201 tcaagcaggg tggccaggag ggccggcagg atcgtcagga gcaggacgaa gacgatgatc
     4261 tggccggcgg tgttgtctct ggcgacgaag aagtcggggt tgttgcccag caggtcgagc
     4321 aggggctgga ccacggccag cgcccagagg gcgagcagat gcaggccgcc catccagatc
     4381 gagggcggtc ccagcggcgc cgcgctctcc gccgtgtcgc tcatcggtcg gcgatcctat
     4441 tgcgtacagg ccgaaactcc gaccccgctg agcggtttca gggacagtta tcagggattt
     4501 gaacccaaaa aacctgaccc agtccgagtc aattctgcag ttcaacccgc cgggggccgg
     4561 gcgatgatgc gccgcgcaat agtcgccctg cttgcagccg cctgcctggc cctctccgcg
     4621 gccggcctgt tggccactgg tgcctcggcg gcgaccaagc ccagcttcgt ggtgatccag
     4681 acggatgacc agccgatgaa cgagtacgac cgccgcttcc ggaatctgta cgacaactgg
     4741 cggccgatca tgccgaagac gatgaaactg atgcgggaca agggcatcac cttcacccag
     4801 tacataacgc ctttcccgct ctgtgcaccc tcgcgggcca ccctgctgag cggtcgctac
     4861 gcgcagaacc acggcgtgat ccg
//
LOCUS       DAOTIP010000051         4834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20881_length_4834_cov_2.948734, whole genome shotgun sequence.
ACCESSION   DAOTIP010000051 DAOTIP010000000
VERSION     DAOTIP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20881_length_4834_cov_2.948734"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="P5138_01625"
     CDS             <1..439
                     /locus_tag="P5138_01625"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRV59307.1"
                     /translation="ADEIVITPSDAFTAITVMTPTMSEYFEQWKLSAFVAGAGEQSEQ
                     GFVATSRLSDIADILTGIDFTYDEIEPLIAAKNPDQAEQTASELSKLLSTSEDLRDRE
                     AAGQKFTPRQADLLGAEMQARAERIAGQVTQAAKDLDIEIQES"
     gene            447..2948
                     /locus_tag="P5138_01630"
     CDS             447..2948
                     /locus_tag="P5138_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59308.1"
                     /translation="MTSASTNSGSARSRVIAAVAAIAATAALMIAAGPADADEGQIPP
                     WRSADQITTSLFDAQTDLILRDPSGGSINQAKRSLSGPLRSGLAASAPAELKQVERQL
                     ARAGRAVASGDSVELAAARGTITASLRRGAFHRAIDLTAADRPAAAREWMQIRDFRQS
                     TRFTRVGTAATGALIALEEGETTSREAVLQVRKDLLDTYQSRLGTNRAEAEKAADRRF
                     PERLAETTAIVDGYWQILAGEYREQRGAEAARRTGREFRQLAVAGAASDLRAFRRADR
                     LTTNDLDGFTAAPFTTAEQARRAAQLTRFLDLIPKEYDHGTDNGRITIPFELQEATAF
                     DDGVEQAFNDLKSDLLERDPQGVAAIERDLALLNRYVNDANERTNVVSLDRVNAVHNR
                     LSDRLDGIFPDEWKESSDEADYDLVDISVDQMTAAISAGQKNLAEQARLSAYAFFEFG
                     PELKLNAFDPQLVTEIEGLFWYGARGVPGLATLVSDGAPTSEVRDTTLELDEALKEAR
                     EKTGEGVSDATAITNAALIVFREGLEAILIIAAITASMLGARAALRKPIYRGALLALP
                     ASLFGFILAVALLGSLAAYGEKVEAVVGVVAIGVLVVVLNWFFHRVYWTEWIATHRKR
                     GKALTGDVALGAAAGTATIAGLYVLGFTSVFREGMETVLFLQALQLSSGVGVVAAGVG
                     LGLAGTGIVGFLTFKAERKLPYKKMLIVTGVLIALVLFTMVGNTVRTLQGVGWFPIHP
                     IDVDIPLWMGTWLGIHPSVETLLAQVLALVFVIGSYWAAGWYSKRQVRIQREQYESEM
                     ARKSDSTPPDSGTPDGTEPLEQGVSGPERRVKEPV"
     gene            3102..4568
                     /locus_tag="P5138_01635"
     CDS             3102..4568
                     /locus_tag="P5138_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRV59309.1"
                     /translation="MSVKKLGISALAVAAAALLVPSIASADVAADLEAKSAELNGTFI
                     ALACVLVLLMQCGFMFLEIGFSRMKNAGAGVMKIFVNFSVCTIAFWAVGAAIIGYGND
                     FIGTHGFFYHFGSEVDDGTGALTAVDSSAVMYMLYSFAFCAVSLAIVWGTTLERIKFA
                     PYIIYAAVFGAIIYPMVGHSVWGGGLLTDVGGKSVMDFAGSSVVHLTGAVGGFAALLL
                     LGARKGKYASDGSPRAIPGHSMPLVGLGVIILWIGWFGFNGGSTLGTDLTFFGEVMWN
                     TQIAAAAGVLGAVIFTYMKTKSLDVGMAGNGAIAGLVAITAPCGFVETWTAPIIGFIA
                     GGVVVFGVLTIEKYLDDPIGALSAHGLAGIWGTLAVGIFASPRLTISAPGIWYGIFGD
                     ADFGSTLGQLGVQALAVVATFTFVFVTSYALFAIIKATVGLRVTESEEDAGLDIASHG
                     MYGYPEAFIPQPELPVGEYTPSMAGTPVASSADTPTQA"
     gene            4587..>4834
                     /locus_tag="P5138_01640"
     CDS             4587..>4834
                     /locus_tag="P5138_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRV59310.1"
                     /translation="MKKIEAFIRHEAFEPIRTDLLEKGIPSMSILEAKGSGRQKGIVE
                     TYRGSTVTVNVRPKLKIEIVVADSEKDLVVETILRHARS"
BASE COUNT          861 a         1582 c         1519 g          872 t
ORIGIN      
        1 cgccgacgag atagtcatca cgccgtccga cgcgttcacg gcgatcacgg tcatgacccc
       61 gaccatgagc gagtacttcg agcagtggaa actttccgct ttcgtcgccg gggccggcga
      121 acagtccgag cagggtttcg tcgccacctc ccggctttcc gacatcgccg acatcctcac
      181 cggaatcgac ttcacctatg acgagatcga gccgctgatc gccgcgaaga accccgacca
      241 ggccgagcag acggcgtcgg agctctcgaa gctgctttcg acctcggaag acctgcgtga
      301 ccgcgaggcg gcaggccaga agttcacgcc gcgccaggca gacctgctcg gggccgagat
      361 gcaggcccgg gcggaacgga tcgccgggca ggtgacccag gccgccaagg acctcgacat
      421 cgagatccag gagagctgat cacaagttga cgtcggcttc gaccaattca ggttctgcca
      481 gaagtcgcgt catcgcggcc gtcgcggcga tcgccgcgac ggccgcactc atgattgccg
      541 ccggtccggc cgacgccgac gaaggccaga ttccgccctg gcgatccgcc gaccagatca
      601 ccaccagcct ctttgacgcc cagaccgacc tgatcctgcg tgacccctcc gggggctcga
      661 tcaaccaggc gaagcgctcg ctttccggtc cgctccgttc cgggctcgcg gccagcgctc
      721 cggcagagct gaaacaggtc gagcggcaac tcgcccgcgc cggtcgtgcg gtcgccagcg
      781 gtgactcggt cgaactcgcc gccgcccggg gcacgatcac cgcatcactt cgtcgtggtg
      841 ccttccaccg cgcaatcgac ctgaccgcgg ccgaccggcc cgcggcggcc cgggaatgga
      901 tgcagatcag ggacttccgc cagagcacga ggttcaccag ggtgggaacg gctgcaaccg
      961 gcgccctgat cgcgctggag gaaggggaga cgacttcgcg ggaggcggta ctccaggtgc
     1021 gcaaggacct gctcgacacc tatcagtccc gtctcggcac caaccgggcg gaggcagaga
     1081 aggcggccga ccgccgcttc ccggaaaggc tggcggagac cactgcgatc gtcgatggct
     1141 actggcagat ccttgccggc gagtatcggg agcagcgtgg tgccgaagct gcccggagga
     1201 cgggtcgcga gttccggcaa ctggccgtcg ccggcgcggc ttcggacctg cgggcattcc
     1261 ggagagccga tcgcctgacc accaatgacc tcgacggctt cacggcagct ccgttcacca
     1321 ccgccgagca ggcccgccgg gccgcccagc tgacccgttt cctcgacctg atcccgaagg
     1381 aatatgacca cggcacggac aacgggcgaa tcaccatccc ctttgaactt caggaggcga
     1441 cggccttcga cgacggggtg gaacaggcct tcaacgacct caagtcggac ctgctggagc
     1501 gggacccgca gggcgtggcg gcaatcgagc gtgacctcgc actacttaac aggtacgtca
     1561 acgacgccaa cgaacggacc aatgtggtca gcctcgaccg ggtcaacgcg gtccacaacc
     1621 ggctttccga ccgcctcgac gggatcttcc ccgacgagtg gaaggagtcc agcgacgaag
     1681 ccgactatga cctggtcgac atcagcgtcg accagatgac cgccgcgatc agcgccggac
     1741 agaagaacct cgccgagcag gcccggcttt cggcctacgc cttcttcgag ttcgggccgg
     1801 agctgaaact gaacgccttc gacccccagc tggtgaccga gatcgaaggc ctcttctggt
     1861 acggcgcccg cggtgttccg gggctcgcca ccctggtttc cgacggggcc ccgacctcgg
     1921 aggtccgtga caccaccctg gaattggatg aagccctgaa ggaagcccgc gaaaagaccg
     1981 gggaaggggt ttcggatgcg accgcgatca ccaacgccgc cctgatcgtc ttccgggagg
     2041 gcctcgaggc gatcctgatc atcgccgcaa tcaccgcttc gatgctcggg gcacgggctg
     2101 cactcaggaa gccgatctac cgaggggcgc tgctcgccct gccggcctcg ctcttcggat
     2161 tcatcctggc ggtagccctg ctgggatccc ttgccgcgta tggcgaaaaa gtggaagcgg
     2221 tcgtcggggt ggtcgcgatc ggggtgctgg tggtggtcct caactggttc ttccaccggg
     2281 tgtactggac cgagtggatc gccacccacc gcaagcgggg caaggccctg accggtgacg
     2341 tcgccctggg cgcggcggcc ggcaccgcaa ccatcgccgg cctctacgtt ctcggtttca
     2401 cctcggtctt ccgcgaaggc atggaaacgg tgctcttcct ccaggccttg cagctctcct
     2461 caggggtggg tgtggtcgcg gccggggtcg ggctgggcct ggccggcacc ggaatcgtcg
     2521 ggttcctcac cttcaaggcg gaaaggaagc tcccctacaa gaagatgctg atcgtgaccg
     2581 gggtcctgat cgccctggtt ctgttcacca tggtcggcaa cacggtccgg acccttcagg
     2641 gcgtcggctg gttccccatt catccgatcg atgtcgacat tcccctctgg atgggcacct
     2701 ggctggggat acatccctcg gttgaaaccc tgctcgccca ggtcctggct ctggtattcg
     2761 tgatcgggag ctattgggcc gccggctggt actcaaagcg gcaggtgcgt atacagcggg
     2821 agcaatacga gtctgaaatg gcccgaaagt ccgattccac tccccccgac tcaggaactc
     2881 ctgacggcac agaacccctt gaacaagggg tttcagggcc cgagcggcgc gtcaaagagc
     2941 cggtctgaag ccactttgac aatcgaacaa ggggtccggg gtcatttcct ccgagtggcc
     3001 aaaattcggc ttggccccgc ttcctagatt tgtccggtca gctaaacgct aatcgcgacc
     3061 ggtgggtttg ggcccagcgg aaacaggagg aaattactcg tatgagtgtc aagaaactcg
     3121 gcatatccgc tctcgcggtt gctgcggcgg cccttttggt gcccagcatc gcgtcggcag
     3181 atgtggcggc cgatctcgag gccaagtcgg ccgagttgaa cggaaccttc atcgcgctcg
     3241 cctgtgtgct cgtgctgctt atgcagtgcg gcttcatgtt cctggagatt ggcttctccc
     3301 ggatgaagaa cgcaggcgcc ggagtgatga agatcttcgt caacttctcg gtctgcacaa
     3361 tcgccttctg ggcggtaggc gcagcgatca tcggttacgg caacgacttc atcggaaccc
     3421 acgggttctt ctaccacttc ggttccgaag tcgatgacgg taccggcgca ctgactgcgg
     3481 tcgattcgag cgcggtcatg tacatgctct acagcttcgc gttctgcgcg gtctctcttg
     3541 cgatcgtctg gggcaccacg ctggagcgaa tcaagttcgc cccttacatc atctacgctg
     3601 ccgtgttcgg agcgatcatc tacccgatgg tcggtcactc cgtctggggc ggcggcctgc
     3661 tcaccgacgt cggtggcaag tccgtgatgg acttcgcagg ttcgtcagtc gtccacctca
     3721 caggcgccgt cggcggtttc gccgcgctgc tcctgctcgg tgctcgaaag ggcaagtacg
     3781 catccgacgg ttcgccacgg gcgatccccg gtcactcgat gccactggtc ggcctcggcg
     3841 tgatcatcct gtggatcggc tggttcggat tcaacggcgg ctcgaccctg ggcacagatc
     3901 tcaccttctt cggcgaggtc atgtggaaca cccagatcgc agctgcggca ggcgtcctgg
     3961 gcgcagtcat cttcacctac atgaagacga agtcgctcga cgtcggtatg gcgggcaacg
     4021 gcgctatcgc cggcctggtc gccatcaccg ctccctgtgg tttcgtcgag acctggacgg
     4081 ccccgatcat cggcttcatc gccggtggcg tcgtggtctt cggagtcctg acgatcgaga
     4141 agtacctcga cgatccgatc ggtgccctct cggcacacgg tctggccggt atctggggca
     4201 ccctggcagt cggcatcttc gcctcgccgc gcctcaccat ttccgcaccg ggcatctggt
     4261 acgggatctt cggtgacgcg gactttggct cgacgctcgg ccagctcggt gttcaggcac
     4321 tggctgtggt cgcgacattc accttcgtgt tcgtcacgtc gtacgcactg ttcgcgatca
     4381 tcaaggcaac cgtcggcctc agggtcacgg aatcggaaga agacgccgga ctcgacatcg
     4441 cctctcacgg catgtacggg tacccggagg ccttcattcc gcagccggaa ctgccggttg
     4501 gcgagtacac cccgtcgatg gccggtacgc cggtcgcatc gtcggctgac acgcccaccc
     4561 aggcctaaca aggaggagat gctcagatga agaagatcga agcattcatt cgtcacgagg
     4621 cgttcgagcc gatccggacg gacctcctcg agaagggaat cccttcgatg tcgatcctgg
     4681 aagcaaaggg ttcgggacga cagaagggga ttgtcgagac ctatcgggga tcgacagtca
     4741 cggtcaacgt ccgtccgaag ctcaagatcg agatcgtcgt ggccgattcc gaaaaagacc
     4801 tcgtcgtcga aacgatcctg cgtcacgctc gatc
//
LOCUS       DAOTIP010000052         4828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20916_length_4828_cov_3.399120, whole genome shotgun sequence.
ACCESSION   DAOTIP010000052 DAOTIP010000000
VERSION     DAOTIP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20916_length_4828_cov_3.399120"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..148)
                     /locus_tag="P5138_01645"
     tRNA            complement(76..148)
                     /locus_tag="P5138_01645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Thr,seq:ggt)
     gene            complement(213..983)
                     /locus_tag="P5138_01650"
     CDS             complement(213..983)
                     /locus_tag="P5138_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRV59311.1"
                     /translation="MTSSNGTTGSARTDRITIGELADRTGMTVRNIRAHQSRGLLDPP
                     KVVGRTGYYDQGHVDRIELIRELQEEGFNLEAIRRIVDSAGEDPGELLRFTRDVRAPY
                     EDEQPEIMSLREIAGPWGDEKFDEERLRRSEALGLMRPLGDGRFEVLSPKLLAAGHEL
                     ASIGVPMDDCLDTLETLKERSTAVAEIFIRLFDEQVWEPFDQAGRPRDRWPEVRDSLR
                     RMRPLASDAFIASFQFAMEEVSEKAISEGIRRDLGEGS"
     gene            1094..2518
                     /locus_tag="P5138_01655"
     CDS             1094..2518
                     /locus_tag="P5138_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRV59312.1"
                     /translation="MNEKSPPKKLTRSRSDRYIGGVAAGLASYLKVDAIFVRIAFIAS
                     LVFGGLGLVAYVLLLAAMPVEGDPDQALPPIEPKRRNMMIGLAVLVGVVGIVTAGSGH
                     FAGWLFGFWPGTLFGILFWTVAVVAAIWLLASGAFARDSTGSGTSTESFPHSDPKPSP
                     DPEAEPVTYAESPTQLPRTEVMETRQMPKQASGNPEDGSSTIGKIMVWFAIGLTALIV
                     FLILFVFSAATTVLAGGIPMAALVIGLGGGMVFAGVRGRRQLSLWLMAAAIAVTIPMA
                     AISIADLRVEGSYGDINETPLTRADIPDDGYKMAAGNMTIDLRKFRFDRNGSINLPIK
                     SGMGLTSVIVPDDVCVTGEVTGKAGVISVRGKQSDGIDVSQSGYSTDLFAGKPDGNGV
                     YKGVVVTSLRPDLVHIDGDFKLGAFEVVDNTQWHADGRAGSFDSNDLEPTDKASAAAR
                     KRATAACQAGIRSLGKKAVKKARP"
     gene            2539..3720
                     /locus_tag="P5138_01660"
     CDS             2539..3720
                     /locus_tag="P5138_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRV59313.1"
                     /translation="MRRDREGGMLAGVCSGIALRFSLDPTLVRVVAVVVTVVTGGVAA
                     IGYGLLWVALPVAGEGPTTRRWKPRQGSRPAGQTPGDRQFKGSWAVAAGAGFVTLGLL
                     FIFRETGVWWSDQLVWPLVIAAAGAALIWRQFSQSTRSLLGPFRGGVGIALLLGGAVL
                     FLYINGALSAGGDVALAGLTAVIVIGLVAAPIWARSTRELNEERAERIRSQERAEVAA
                     HLHDSVLQTLALIQKNPEDSRRISTLARSQERELREWLSGKTESERADSLAESLKVVA
                     AEVEERHQVPIDAVTVGDRPLDEDSIALVAASREAMTNAARHAGQAGPVRLYAEIDPK
                     RTEVFIHDRGPGFDPEEVPDDRRGVRDSILGRMKTHGGSAEIRSTPGEGTEVELVLET
                     K"
     gene            3717..4430
                     /locus_tag="P5138_01665"
     CDS             3717..4430
                     /locus_tag="P5138_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV59314.1"
                     /translation="MSDAEKPGGTGADDAGAGGRVRVAIVDDHAIFRAGVRSELVAGV
                     EVVGEADGVADAGLLIARTRPDVVLLDVHMPDGGGVEVIRRAAEAAAADPEASPAPRF
                     LALSVSDDPEDVVSVIRAGARGYVTKSISGPDLIDAIGRVDQGDAVFSPQLAGFVLDA
                     FAGATAPEEDNELDLLTPREQEVLRLIARGYMYKEIARRLGISVKTVEAHTSSTLRKL
                     QLSSRHELARWATERDLLD"
     gene            complement(4431..>4828)
                     /locus_tag="P5138_01670"
     CDS             complement(4431..>4828)
                     /locus_tag="P5138_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HRV59315.1"
                     /translation="ELARGFEVLRKGSLQLASKMRFVSAQLNAQLEDGLWREIADHSN
                     SMARLLADGIEWTEGADLGQSVQANIVFMSMPIGPARKLVESCAPNAPLLFEAGEAGV
                     IRLVCSWDTQPEDIDHFLGLLREALPADH"
BASE COUNT          800 a         1644 c         1608 g          776 t
ORIGIN      
        1 agtgcagaaa atggagcgga tatgcggggc tggtttgaga tgccccagcg gccgcatccc
       61 gcatggtggt gggcaagccc ggaagcggat tcgaaccgct gaccccttcc ttaccatgga
      121 agtgctctac ctactgagct atccgggcgg gacgggaaac tttagcgggg aagcggcggt
      181 tcggtgtttc cggcaccgga ccgggacggc cgtcaggacc cttccccgag gtcgcgccgg
      241 atcccctccg agatcgcctt ctcggaaacc tcctccatcg caaactggaa cgaggcgatg
      301 aaggcgtcgg atgcgagcgg tcgcatgcgg cgcagcgagt cgcgaacctc gggccagcgg
      361 tcgcgggggc ggccggcctg gtcgaacggt tcccagacct gctcgtcgaa gagccggatg
      421 aagatctcgg ctaccgcggt cgacctctcc ttcagggtct cgagcgtgtc gaggcagtca
      481 tccatcggca ccccgatcga ggccagttcg tggccggccg cgagcagttt cggcgaaagc
      541 acctcgaagc gcccgtcgcc cagcgggcgc atcagcccca gcgcttcgga gcggcgcagt
      601 ctttcctcat cgaatttctc gtcaccccag gggccggcga tctcccgcag cgacatgatc
      661 tccggctgct cgtcctcgta gggcgcacgg acgtcacggg tgaacctgag gagctccccc
      721 gggtcctcgc cggcgctgtc gacgatccgc ctgatcgcct cgagattgaa cccttcctcc
      781 tgcagctccc ggatcagttc gatgcggtcg acatggccct ggtcgtagta gccggtcctc
      841 cccaccacct tcggcgggtc gagaagtccc cgcgactggt gggcacggat gttccgcacg
      901 gtcatgccgg tgcgatcggc gagttcaccg atcgtgatcc gatcggttcg cgcgctgccg
      961 gttgtcccgt tgctggaagt cacctgagaa gatcgtaagc cgaaaacggt tatgaccccc
     1021 ggggtcgacc ctgatgccat ctaagggtca ttcccaatcg acaggaccgg cccgggcatt
     1081 cagtctcacg ggcatgaacg aaaagtctcc acccaagaaa ctaacccgct cccgttccga
     1141 ccgctacatc ggcggcgtcg cggccggtct cgccagctac ctgaaggttg acgcaatctt
     1201 cgtgcggatc gccttcatcg ccagcctcgt gttcggcggc ctcgggctgg tcgcctatgt
     1261 cctccttctg gccgcgatgc cggtcgaggg cgacccggac caggcgctgc ctccgatcga
     1321 accgaaacga cgcaacatga tgatcggcct cgccgtactt gtcggggtcg tcggcatcgt
     1381 gaccgccggc tccggccact tcgccggctg gctgttcggc ttctggccgg gcaccctctt
     1441 cgggatcctc ttctggaccg tggcggtggt tgctgccatc tggctgctcg catccggggc
     1501 tttcgcccgg gactcgaccg gctccgggac gagcaccgaa tcgttccccc actcggaccc
     1561 gaaaccatcg cctgacccgg aggcggaacc ggtcacctat gcggaatccc ccacccagct
     1621 gccccgcaca gaggtgatgg aaaccaggca gatgccgaag caggcgtccg gcaatcccga
     1681 agacggctcg tcgacgattg gcaagatcat ggtctggttc gcgatcggac tgaccgcgct
     1741 gatcgtcttc ctgatcctct tcgtcttttc cgccgccacg accgtgctcg ccggcggcat
     1801 cccgatggcc gccctggtga tcggcctcgg tggcggcatg gtcttcgccg gagtgcgcgg
     1861 acgccgccag ctctcgctct ggctgatggc cgcggccatc gccgtgacca tcccgatggc
     1921 cgccatctcg atcgccgacc ttcgggtcga gggcagctac ggcgacatca acgaaacccc
     1981 gctcaccagg gcggacatcc ccgacgacgg ctacaagatg gccgccggaa acatgaccat
     2041 cgacctgagg aagttccgct tcgatcggaa cggctcgatc aatctcccga tcaagagcgg
     2101 catgggcctc acttccgtga tcgtccccga tgacgtctgc gttaccggtg aggtaaccgg
     2161 caaggcggga gtcatctcgg tgcggggcaa gcagtcagac ggaatcgacg tctcgcagag
     2221 tggctattcg accgacctct tcgccggcaa accggacggg aacggggtct acaagggcgt
     2281 cgtggtgaca agtctgcggc cggacctggt tcatatcgac ggagatttca agctcggcgc
     2341 cttcgaagtg gtcgacaaca cgcagtggca tgctgacggc cgggcaggca gcttcgactc
     2401 gaatgacctc gaaccgactg acaaggcctc tgccgccgcc cgcaagagag caactgccgc
     2461 ctgccaggcc ggaatccggt cactcggcaa gaaggccgtc aagaaggcgc gcccctgaac
     2521 gactcccctg acaagcggct gaggcgggac cgtgagggcg ggatgcttgc cggagtctgc
     2581 tccggcatcg ccctccggtt cagcctcgac ccgaccctgg tccgggtcgt cgccgttgtc
     2641 gtgaccgtgg tcaccggcgg ggttgcggcg atcggctacg ggctgctctg ggtggccctg
     2701 ccggtcgccg gcgaaggacc gaccacccgc cgctggaagc cgaggcaagg aagccgcccg
     2761 gcaggccaga cacccgggga ccggcagttc aagggctcct gggcggtcgc cgcgggggcc
     2821 ggcttcgtca ccctcgggct gctgttcata ttccgcgaga ccggggtctg gtggtcggat
     2881 cagctggtct ggccgctggt catcgcggcg gccggcgccg ccctgatctg gcggcaattc
     2941 agccagtcga cccgttcgct gctcggcccg ttccggggcg gggtcggcat cgccctgctg
     3001 ctcggcggcg ccgtgctctt cctctacata aacggggccc tcagcgctgg cggcgatgtc
     3061 gccctggccg gactgaccgc ggtgatcgtg atcggattgg tcgccgctcc gatctgggcg
     3121 cgctcgaccc gggaactcaa cgaggaaagg gccgagcgca tccgctcgca ggagcgggcc
     3181 gaggttgccg cccacctgca cgactcggtc ctccagaccc tggccctgat ccagaagaac
     3241 cccgaggaca gccgccggat ctccaccctc gcccgctccc aggaacgcga gttgcgcgaa
     3301 tggctctccg gcaagaccga gagcgagcgg gcggattccc tggccgagtc gctgaaagtc
     3361 gttgccgccg aggtggagga aaggcatcag gtaccgatcg acgcggtgac ggtcggcgac
     3421 cggcccctgg acgaggactc gatcgccctg gtcgccgcca gccgggaggc gatgaccaat
     3481 gccgcccgcc acgccggcca ggccggaccg gtccggctct acgccgagat cgatccgaag
     3541 cggaccgagg tcttcatcca cgaccgggga ccgggcttcg acccggaaga ggttcccgac
     3601 gaccgtcgcg gggtgcgaga ctcgattctc ggaaggatga aaacccacgg gggcagcgct
     3661 gagatccggt ccacccccgg tgagggaacc gaagtcgaac tggtgctgga gacgaaatga
     3721 gcgacgcaga gaaacccggc gggaccgggg cggatgatgc cggtgccgga ggccgggtcc
     3781 gggtcgccat cgtcgacgac cacgcaatct tccgggccgg ggttcgctcc gaactggtag
     3841 ccggggtgga agtggtcggg gaggccgacg gggtcgccga cgccggactc ctgatcgccc
     3901 ggaccaggcc ggacgtcgtc ctgctcgacg tccacatgcc ggacggcggc ggggtcgaag
     3961 tgatcaggcg ggcggccgaa gctgccgccg ccgatcccga ggcgagcccc gccccgaggt
     4021 tcctcgcgct ttcggtgtcg gatgacccgg aggacgtggt cagcgtgatc cgggccgggg
     4081 cccgcggata cgtgaccaag tcgatttccg gacccgacct gatcgatgca atcggcaggg
     4141 tcgaccaggg tgacgcggtc ttctcgccgc agctggccgg cttcgtactc gacgccttcg
     4201 ccggagccac cgccccggag gaggacaacg aactcgacct gctcaccccg cgcgagcagg
     4261 aggtcctgcg actgatcgcc cgcggctaca tgtacaagga gatagcccgc cgcctcggca
     4321 tctcggtcaa gacggtcgag gcccacacca gctcgaccct ccgcaagctg cagctctcga
     4381 gccgccacga actggcccgc tgggcaaccg agcgcgacct gctcgactga tcagtggtcg
     4441 gccgggagcg cttcgcggag caggccgagg aaatgatcga tgtcttcggg ctgcgtgtcc
     4501 caggagcaga ccagcctgat cactcctgcc tccccggcct cgaagagcag cggggcattg
     4561 ggcgcacagg actcgaccag cttccgggcc gggccgatcg gcatcgacat gaaaacgatg
     4621 ttcgcctgga ccgactggcc gaggtcggcg ccttccgtcc attcgatccc gtcggcgagc
     4681 agccgggcca tcgagttgga gtgatcggcg atctcccgcc acaacccgtc ctcgagctgg
     4741 gcgttcagct gggcggaaac gaagcgcatc ttcgaggcga gctggaggct gcccttgcgg
     4801 agcacctcga aacccctggc aagttcag
//
LOCUS       DAOTIP010000053         4814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_20989_length_4814_cov_3.484766, whole genome shotgun sequence.
ACCESSION   DAOTIP010000053 DAOTIP010000000
VERSION     DAOTIP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20989_length_4814_cov_3.484766"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..296)
                     /locus_tag="P5138_01675"
     tRNA            complement(223..296)
                     /locus_tag="P5138_01675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Pro,seq:tgg)
     gene            complement(448..1404)
                     /locus_tag="P5138_01680"
     CDS             complement(448..1404)
                     /locus_tag="P5138_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV59316.1"
                     /translation="MKRVLVTGGNGFLGHSVVRGLAEATDQVELVVSADLGHPGDDRR
                     VEGVDYVVADVTDASAVSGPIGEHGIDTVVHLASIVNPGANTTVEQEFAVDVTGSKNV
                     FDACLAHGVKRVVVSSSGAAYGYHADSPAWLTEDDPVRGTDTFPYSLHKRLVEEQLAA
                     LRESNPELEQVVLRIGTILGETVDNQITALFKAKRPLKIRGTELPFVFIWDQDVVGVI
                     LQAVLGDKTGIYNVAGDGAMTIDEISAELGKKPLAVPVSALKAGISIGHFLHLTPHGP
                     AQTDFLAYRPVLDNRKLKEEFGYTPKFTTREAFHAWRLSQGL"
     gene            complement(1418..2308)
                     /locus_tag="P5138_01685"
     CDS             complement(1418..2308)
                     /locus_tag="P5138_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRV59317.1"
                     /translation="MVIILSLIIAVIMFGIALEMKVDDFRTVKDAPKAMAVGIIAQFV
                     LLPAITYLLTVALGFRPSIAMGMILVACCPPGNVSNILVYRARGNVALSLSMTAVSNF
                     LAIFLMPLNIAFWGGMHPEASKVLQDVNVSAIELLFDIIVLIGIPLTIGLFVSSRHPE
                     IAKKVQPLVKQVSMIFLILFIVGALASNFSAFINHIALIAVAVFLHDSLALLLGYGIA
                     ASFRVAEPNRRAITFEVGVRNAALGLTIALATFKGIGGVAMVAAWWGVWDLIAGLILS
                     SWWARRPTGDPVDSTIEARA"
     gene            complement(2383..3717)
                     /locus_tag="P5138_01690"
     CDS             complement(2383..3717)
                     /locus_tag="P5138_01690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV59318.1"
                     /translation="MAPEGDANNELPWALIGAGPTGLSAARNLSRRGIPFVGFEIHTD
                     VGGLWDITSKKSTMYESAHLISSKTTTAYDEFPMRDEVADFPSHWEMNTYFKDYADTF
                     DLRRNYRFGTEVIGAEPEDPDSDDSVWKVTIRNEDGEEETGRYAGVIIANGILSEPSV
                     PEFEGQDEFEGEIVHTASYKRPDVFRDKKVLIIGCGNSGCDIAVDAVHYAESIEMSLR
                     SGYWFFPKYIFGRPSDTLNEGKALPAFLKTRIDGKFIKFLTGDPARLGFPVPEGRIYE
                     THPVMNTQVLYYAGHGDIHIRKNIKRFTKTGVEFVDGSTGDYDIVMLATGYKLHYPFI
                     DPEYLNWKGNSPDLYLNIFTPKFRNVFVLGMVEATGLGWQGRYNQAHLVSEFIDAGER
                     DPAKAEEMWRRVQGPEPDLFAGYHYRQMDRLPFYVNKDAYRAQIHEHLRIFE"
     gene            3782..>4814
                     /locus_tag="P5138_01695"
     CDS             3782..>4814
                     /locus_tag="P5138_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRV59319.1"
                     /translation="MAEVRSFPALHYDLNAVGNLTDVVAPPYDVIDGAERASLLARSP
                     FNVVEIDLPESPGGDRYEHAAGTMEEWILSGILHQDRDPSVWALTQEYIGPDGSMRIR
                     NAVLARVRVEDYGPGRIRPHERTQPGPKQDRLDLTHATRFNLSPIFSLTTRDAWPAVA
                     PAIAGEDPWAEVLDGEGTLNRIWRISDPEVHRAVSQELDEAELLIADGHHRYETARVY
                     RDQVGGEGAHCYTLMALTALDDPGLTVFPTHRLLTGLDDPDLVDRLNRGIEGAFETVG
                     ENEGRGDVDPAGLEGAGCLGLILADGTRRLLRLRDPDSLGALFPDHSPAYRELDAAIL
                     EKVVLDGVLG"
BASE COUNT          885 a         1501 c         1553 g          875 t
ORIGIN      
        1 cggggtggaa gctaaccctt cgaataccgc tttgcagtgc ggttttttgc tccttttgta
       61 agtcgtgaga actcgtgaga aagttctcac cactttcgca ctatgccctt tggaaacttc
      121 ttcgcggggc ttcgcaaggc ttcgctgggg aaccccaatg gaatggggga aactcccgtc
      181 cacttcgcga tttttcgctc ctcgcactat ggagcgaaca aatcggggcg actggatttg
      241 aaccagcgac cccttgctcc caaagcaagt gcgctaccag gctgcgccac gccccgacgc
      301 gacgacgatt ctacttccag cagccacaga gcagatgccc cgttgctccg tgtaagcgag
      361 gttgaaatgg agagcgctat ttgcacttga tttcaatctc gcttcagagc agttgagaac
      421 ccgggcgggc gggaccttga tcggggttca gagaccctgc gagaggcgcc aggcgtggaa
      481 cgcttctcgg gtggtgaact tcggggtgta gccgaactct tccttgagct tccggttgtc
      541 caggaccggc cgataggcga ggaagtcggt ctgggccggc ccatgcgggg tcaggtggag
      601 gaaatggccg atcgagatac ccgccttcag ggcgctgacc ggaacggcga ggggcttctt
      661 gccgagttcg gcggagatct cgtcgatcgt catcgcgccg tcgccggcca cgttgtagat
      721 gcccgtcttg tccccgagca ccgcctgcag gatcaccccg accacgtcct ggtcccagat
      781 gaaaacgaag ggcaattcgg tgccgcggat cttcaacggc cgcttcgcct tgaagagggc
      841 ggttatctgg ttgtccacgg tctcgccaag gatcgttccg atccggagca ccacctgctc
      901 gagctccggg ttcgattccc ggagcgcggc cagctgctcc tcgaccagcc gcttgtgaag
      961 gctgtagggg aaggtgtcgg tgccgcggac cgggtcgtcc tcggtcagcc aggccgggct
     1021 gtcggcgtgg tagccgtagg ccgcaccgct ggaggaaacg acgacccgct tcaccccgtg
     1081 agcgaggcag gcgtcgaaga cgttcttcga cccggtcacg tccaccgcga actcctgctc
     1141 gaccgtggtg ttggcgcccg gattgacgat cgaggcgagg tgaaccaccg tgtcgatccc
     1201 gtgttcgccg atcggaccgg aaaccgccga agcgtcggtg acgtcggcca ccacatagtc
     1261 gaccccctcc acccgccgat cgtcgccggg atgacccagg tcggcggaaa cgaccagctc
     1321 gacctgatcg gtcgcctcgg ccagaccgcg caccaccgag tgcccgagaa atccgttgcc
     1381 gccggtgacc agaacgcgct tcatgatttc cccactttca ggccctggcc tcgattgtgc
     1441 tgtcaaccgg gtcaccggtc ggccgtctcg cccaccaact ggagaggatc aggcctgcaa
     1501 tgaggtccca gaccccccac caggcagcca ccatcgcgac cccgccgatc cccttgaagg
     1561 tggccaatgc aatggtcagg ccaagggccg cattgcgcac cccgacctcg aaagtgatcg
     1621 cccgccggtt cggttcagcg acccggaacg aagccgcgat cccgtaacca agtaacaggg
     1681 ccaggctgtc gtggagaaag accgctaccg caatcagggc gatgtggttg atgaaggcgc
     1741 tgaagttact ggccagcgct ccgacgatga acaggatcag aaagatcatg ctcacctgct
     1801 tgaccagcgg ctggaccttc ttggcgatct ccggatgccg gctggaaacg aagagtccga
     1861 tcgtgagcgg aataccgatc aggacgatga tgtcgaagag cagctcgatc gcactgacgt
     1921 tcacgtcctg cagcaccttg gatgcctccg ggtgcatgcc tccccagaaa gcgatgttca
     1981 ggggcatcag gaagatcgcc aggaaattgg aaacggcggt catcgagagg gataaggcca
     2041 cgtttccgcg cgcccgatag acgaggatgt tcgagacgtt tcccggtgga caacaggcga
     2101 ctaaaatcat ccccatcgct atcgaggggc ggaagccgag ggccacggtc aacaggtagg
     2161 tgatagccgg caggaggacg aactgggcga tgatgccgac cgccatcgcc ttcggagcat
     2221 ccttgaccgt cctgaagtca tcgaccttca tctccagggc gatgccgaac atgatcaccg
     2281 cgatgatcag gctcaaaatg atgaccatgc cgtcgctcgt gtggagctgg acgttgtcta
     2341 tgtcctccgc cccgagcggc aggctggtca ggaaggcgag gcttactcga agatcctcag
     2401 gtgctcatgg atctgggccc ggtaggcgtc cttgttcaca tagaagggga gccggtccat
     2461 ctggcgatag tggtagccgg cgaagaggtc gggctcgggc ccctggaccc tgcgccacat
     2521 ctcctcggcc ttggccggat cgcgctcgcc cgcgtcgatg aattcggaga ccagatgggc
     2581 ctggttgtag cggccctgcc agccgaggcc ggtcgcctcg accatgccga gcacgaagac
     2641 gttgcggaac ttcggagtga agatgttcag gtacagatcc gggctgttgc ccttccagtt
     2701 caggtactcc ggatcgatga acgggtagtg gagcttgtag ccggtggcga gcatcacgat
     2761 gtcgtagtca ccggtcgacc cgtcgacgaa ctcgaccccg gtcttcgtga accgcttgat
     2821 gttcttgcgg atgtgaatgt ccccgtgacc ggcgtaatag agcacctggg tgttcatcac
     2881 cgggtgggtt tcgtagatcc ggccttccgg caccgggaaa ccgagtcggg ccgggtcgcc
     2941 ggtcaggaac ttgatgaact tgccgtcgat ccgggtcttg agaaaagccg gcagggcctt
     3001 gccctcgttc agggtgtcgg aaggccggcc gaagatgtac ttggggaaga accagtagcc
     3061 ggagcgcagg ctcatctcga tcgactccgc gtagtggacc gcgtccaccg cgatgtcgca
     3121 accggagttg ccacagccga tgatcagcac cttcttgtcc cggaagacat cgggacgctt
     3181 gtaggaggcc gtatggacga tctcgccctc gaactcgtcc tggccctcga actcggggac
     3241 gctgggctcg gaaaggatgc cgttggcgat gatcaccccg gcgtagcgac cggtctcctc
     3301 ttccccgtcc tcatttctga tcgtcacctt ccagaccgag tcgtccgagt ccgggtcttc
     3361 gggttcggcg ccgatgacct cggtgccgaa gcggtagttc ctccgcaggt cgaaggtgtc
     3421 ggcgtaatcc ttgaaatagg tattcatctc ccagtgactg ggaaagtcgg ccacctcgtc
     3481 gcgcatcggg aattcgtcgt aggcggtggt ggtcttcgag gagatcaggt gggccgactc
     3541 gtacatcgtc gacttctttg aggtgatgtc ccagagcccg ccgacgtcag tgtggatctc
     3601 gaatccgacg aaggggatgc cacggcgcga gaggttgcgg gcggcggaga gtccggtcgg
     3661 cccggctccg atcaatgccc acggcagttc gttgtttgcg tccccctcag gcgccacggt
     3721 ggccaatcct atggcaagga agaaaaagca ccgcggctcc ggggcgctac cgtgaaagtc
     3781 aatggctgaa gtgcggtcct tcccggcgct ccactacgac ctcaacgcgg tcggcaatct
     3841 gaccgacgtg gtggctcccc cctacgacgt gatcgacggg gcggagcggg cgtcgctgct
     3901 cgcccgttcc cctttcaacg tggtcgagat cgacctgccg gaatcacccg gcggggaccg
     3961 ctacgaacac gcggcgggaa ccatggagga gtggattctg agcgggatcc tgcaccagga
     4021 ccgcgacccg tccgtgtggg ccctgaccca ggagtacatc ggcccagacg gatcgatgag
     4081 gatccgcaac gccgtcctcg cccgggtgcg agtcgaggac tacggcccgg gccggatccg
     4141 gccgcatgag aggacccagc cgggcccgaa acaggaccgg ctcgacctga cccacgcgac
     4201 ccggttcaat ctctccccga tcttctccct gaccacgcgt gacgcatggc cggccgtggc
     4261 gccggcgatc gcgggcgagg atccatgggc cgaggtcctc gacggcgagg gaacgctgaa
     4321 ccggatctgg cggatctccg atcccgaggt tcaccgggcg gtgagccagg agctggacga
     4381 ggccgagttg ctgatcgccg acggccacca ccgttacgag accgcccgcg tgtaccgcga
     4441 ccaggttggc ggtgaaggcg cccactgcta caccctgatg gcgctgaccg cgctggatga
     4501 tcccgggctg accgtattcc ccacccaccg gctgctgacc ggcctggatg atccggatct
     4561 ggtcgatcgc ctgaataggg gtatcgaagg ggcgttcgaa acggttggcg agaacgaggg
     4621 caggggcgac gtcgaccctg ccgggctgga aggcgcagga tgcctcggcc tgatcctggc
     4681 cgacggaacc cggcggctgc tgcggcttcg cgaccctgac tctctcggtg ccctgttccc
     4741 cgatcactcg cccgcttacc gggaactcga cgcggcgatc ctcgagaagg tcgtcctgga
     4801 tggggtcctc ggga
//
LOCUS       DAOTIP010000054         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_21012_length_4810_cov_3.367613, whole genome shotgun sequence.
ACCESSION   DAOTIP010000054 DAOTIP010000000
VERSION     DAOTIP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21012_length_4810_cov_3.367613"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="P5138_01700"
     CDS             <1..756
                     /locus_tag="P5138_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59320.1"
                     /translation="KISPSPSPKTICDPFQKASIRCLQNKTTELNGTSSDLIFTQVTK
                     PITVGANSTATESLVCGDNAKGIVGGWEFEDGLVPLGNDPQPKTRVFYVWNPTDHPLT
                     GTLYLLCLEARTGSSVPVGEKTYVNTATVSSSSTQDSGAVLSDDASVKVTRDNHYAAP
                     SIYKATLTGRKLTVGFKSATRKGRVAVSLPGKSRLIGKARFRLGKAGKGKTKVKLTRR
                     AARAIHKGKIRKVKVKVTSANGKTRVKTLRVKH"
     gene            complement(837..1796)
                     /locus_tag="P5138_01705"
     CDS             complement(837..1796)
                     /locus_tag="P5138_01705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRV59321.1"
                     /translation="MGERSRVVILRELLPAGRELLSDRFEVSSLGLDAGWQQMLAAAA
                     GADGLVTDLTFAVDGELLDAAGPQLKVVANFGVGYDNVDLAACAERGVAVTNTPDALT
                     NATAELALTLAMAAARDLPGAEKDLREGRWQGWDPARWRGFELSGATIGVVGAGRIGG
                     RFMELVAGFGGERIYTSRQPKPELEARFGAERVGLDDLLRRSDLVSLHLPGTPETRHL
                     IDGRALGLMKPTAVLVNTGRGTLVDSKALILALEEDRIGAAGLDVFEGEPNFDPGFLT
                     TPRVALAPHIGSATYRSRDEMSRLVAANVIAVLEGREPPNPVT"
     gene            complement(1811..2797)
                     /locus_tag="P5138_01710"
     CDS             complement(1811..2797)
                     /locus_tag="P5138_01710"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRV59322.1"
                     /translation="MSSTAKVTVTGAAGQIGYALLFRIASGQMLGPDTKVHLSLLEIP
                     AAVKAAEGTAMELDDCAFPLLDKISISDDPKEAFDGVNIGLLVGARPRGPGMERADLL
                     EANGGIFKPQGEAINAGAADDVKILVVGNPANTNALIAASAAPDVPKERFTAMMRLDH
                     NRAIAQLANKLGRPVSDIKRMTVWGNHSATQYPDISHALVDGAPAPAQVDDEVWVREE
                     FIPRVAKRGAEVIDARGSSSAASAANAAIDHVRDWVLGTPDEDWVSMGIPSDGSYDVP
                     EGIIAGFPVTCANGEYSIVQGLEIDEFSRGKIDANAAELQDERSAVADLGLV"
     gene            complement(2827..4041)
                     /locus_tag="P5138_01715"
     CDS             complement(2827..4041)
                     /locus_tag="P5138_01715"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRV59323.1"
                     /translation="MSKIKVTNPIVELDGDEMTRIIWSFIKEQLILPYLDVDLKYYDL
                     GIESRDATDDQITVDAANAIKEHGVGVKCATITPDEARVEEFGLKEMYRSPNGTIRNI
                     LGGVIFREPIVISNVPRLVPSWTKPIVIGRHAFGDQYRATDLVVPGEGTLTMAFTPKD
                     GAEPIEMHVHDFPGGGVAMAMYNHDDSIRDFARASFVYGLERGFPVYMSTKNTIMKRY
                     DGRFKDLFQEIFDNEFKEQFDAAGLTYEHRLIDDMVASALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRLHQKGEKTSTNPIASIFA
                     WTRGLSARGRMDGTPEVSEFAETLERVCIETVESGKMTKDLARLIDADHPWQTTQEFL
                     ESIDENLQKAMA"
     gene            4172..>4810
                     /locus_tag="P5138_01720"
     CDS             4172..>4810
                     /locus_tag="P5138_01720"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HRV59324.1"
                     /translation="MPETAETPQHATHTVFNQSTPLENVNLYEIDLPLREAVRREGAD
                     WAEDRIRALGEICGQPDTLLAGRDANEFPPRLKAFDRFGNRIDEVEFHPAWHQLMSLG
                     IEHGLHALPWREPGQGANVARAALFSQLTQVEAGVGCPISMTFSVIPSLRNQPDVAEE
                     WEPRFTSLEYDGRLRPPEEKPGCLAGMAMTEKQGGSDVRANTTRAVALNGGGP"
BASE COUNT          819 a         1582 c         1570 g          839 t
ORIGIN      
        1 aagatctcgc cgtccccgtc gccgaagacc atctgcgacc cattccagaa ggcgagcatc
       61 cgctgcctcc agaacaagac gaccgagctg aacggcacct cttcggatct gatcttcact
      121 caggtcacga agccgatcac ggtcggtgcc aacagcacgg ccacggaatc gctggtctgc
      181 ggcgataacg ccaagggcat cgtcggtggc tgggaattcg aggacggcct ggtgccgctc
      241 gggaacgacc cgcagccgaa gacccgtgtg ttttacgtct ggaacccgac cgaccatccg
      301 ctgaccggca cgctttatct gctctgcctg gaagcgcgca ccggctcatc ggtcccggtc
      361 ggcgagaaga cctacgtcaa cacggccacg gtcagctcat cgagcaccca ggactcgggc
      421 gcggttctct ccgatgacgc ctcggtcaag gtgacccgtg acaaccacta cgccgcacct
      481 tcgatctaca aggccaccct gaccggccgc aagctgacgg tcgggttcaa gtccgccacc
      541 cgcaagggca gggtcgcggt gagcctgccc ggcaagagcc gcctgatcgg caaggccagg
      601 ttccgtctcg gcaaggccgg caagggcaag acgaaggtca agctcactag gcgtgccgcc
      661 cgtgcgatcc acaagggcaa gatccggaag gtcaaggtga aggtcacctc ggccaacggc
      721 aagacccggg tcaagaccct gcgggtcaag cactagtttc acgtccagaa cgaagaccgc
      781 agctccgcgg tccaggggcc tcgatccgga gagggatcgg ggcccctttt tcttgctcag
      841 gtgaccgggt tgggcggttc ccgcccctcc agtaccgcga tcacattggc ggctacaagg
      901 cgggacatct cgtcacgcga gcggtaggtc gcggagccga tgtgaggggc cagggcgacc
      961 cttggcgttg tcaggaagcc agggtcgaag ttgggctccc cctcgaaaac gtcaagcccc
     1021 gcagcgccga tccggtcttc ttccagggcc aggatcaggg ccttcgagtc gaccagggtt
     1081 ccgcggccgg tgttgaccag caccgcggtc ggcttcatga gtcccagggc ccgtccgtcg
     1141 atcaggtgcc tggtctccgg ggttcccggc agatggaggc tgaccagatc ggaacggcgg
     1201 agcaggtcgt ccaggccaac ccgttccgcc ccgaagcgcg cttccagttc aggcttgggt
     1261 tgccgggagg tgtagatccg ttcgcctccg aaacctgcca ccagttccat gaagcggcca
     1321 ccgatccggc cggcgccgac caccccgatg gtggcaccgc tcagttcgaa tccacgccac
     1381 cgggccgggt cccagccctg ccacctgcct tcgcgcaggt ccttctcggc ccccggcagg
     1441 tcacgcgcgg cggccatcgc gagggtgaga gcgagttcgg ccgtggcgtt ggtcaaagca
     1501 tcgggcgtgt ttgtgacggc aaccccgcgt tcggcgcagg cggccaggtc gacgttgtcg
     1561 tatccgaccc cgaagttggc caccaccttc agctgtggac cggcggcgtc aagcaactca
     1621 ccgtcgaccg caaaggtcag gtcggtgacg agaccgtcag ccccggccgc cgccgcgagc
     1681 atctgttgcc agcccgcatc gaggccgagg gaagaaacct cgaaacgatc cgagaggagc
     1741 tcccgcccgg ctggcaggag ctcccgaagg atcacgactc tggatcgctc gcccatcggc
     1801 gaagcgtggc ctagaccagg ccgaggtctg cgaccgcgct gcgctcgtcc tgcagctcgg
     1861 ctgcgttggc gtcgatcttc ccgcgcgaga attcgtcgat ctcgaggccc tgaacgatcg
     1921 agtactcgcc gttggcgcag gtaacgggga agccggcgat gatgccctcc ggcacgtcgt
     1981 aggagccgtc cgacgggatg cccatcgaga cccagtcctc gtcgggggtg ccgagcaccc
     2041 agtcgcggac gtggtcgatc gcggcgttgg ctgccgaggc cgcgctggag gaaccacggg
     2101 catcaatcac ctctgccccg cgcttggcga cccgggggat gaactcctcg cggacccaga
     2161 cctcgtcgtc aacctgggcg ggggcgggag caccgtcgac gagggcgtgg ctgatgtccg
     2221 ggtactgggt ggccgagtgg ttgccccaga cggtcatccg cttgatgtcg gaaaccggcc
     2281 ggccgagctt gttggccagc tgggcgattg cccggttgtg atcgaggcgc atcatcgcgg
     2341 tgaagcgttc cttcggtaca tccggggccg ccgaagcggc gatcagggcg ttggtgttgg
     2401 ccgggttgcc gaccaccagg attttcacgt catcggcagc gccggcgttg atcgcctcgc
     2461 cctgcggctt gaagatcccg ccgttggctt cgagcaggtc cgcccgctcc attcccgggc
     2521 cgcgcggccg ggcgccgacc agcaggccga tgttgacacc gtcgaaggcc tctttcgggt
     2581 catccgaaat gctgatcttg tcgagcagcg ggaaggcgca atcgtccagc tccatggcgg
     2641 tgccctcggc ggccttcacg gcagccggaa tctcgagcag gctcaggtgg accttggtgt
     2701 cggggccgag catctgtccg ctggcgatcc ggaagagcag ggcgtaaccg atctggccgg
     2761 cggcgccggt cacggttacc ttggcggttg agctcacgtt cttctccttg gtttctggtg
     2821 gcgggcctaa gccatcgcct tctgaaggtt ttcgtcgatc gactccagga actcctgggt
     2881 cgtctgccac gggtggtcgg cgtcgatcag ccgcgccagg tccttggtca tcttgccgct
     2941 ctcgaccgtc tcgatgcaga cccgctcgag ggtctcggcg aattcgctca cctcgggggt
     3001 gccgtccatc cggccacggg ccgaaaggcc gcgggtccag gcgaagatcg aggcgatcgg
     3061 attggtcgaa gtcttctcgc ccttctgatg caggcggtag tggcgggtca cggtaccgtg
     3121 ggcggcctcg gcctcgaccg tcttgccgtc cggggtcatc aggaccgagg tcatcagtcc
     3181 gagcgagccg aagccctggg caacggtgtc cgactggacg tcgccgtcgt agttcttgca
     3241 ggcccagacg taaccgccct cccacttcag ggcggaggcg accatgtcgt cgatcagccg
     3301 gtgctcatag gtgagtcctg cagcatcaaa ctgctccttg aactcgttgt cgaagatctc
     3361 ctggaagaga tccttgaagc ggccgtcgta gcgcttcatg atcgtgttct tggtcgacat
     3421 gtagaccggg aagccgcgct cgaggccgta aacgaacgaa gcccgcgcaa agtcacggat
     3481 cgagtcgtcg tggttgtaca tggccatcgc tacgccgccg ccggggaagt catggacatg
     3541 catctcgatc ggctcggcac cgtccttcgg ggtgaaggcc atggtcaggg ttccctcacc
     3601 cggaaccacc aggtccgtgg cgcggtactg gtcaccaaaa gcatgacggc cgatcacgat
     3661 cggcttggtc cagctcggca ccagccgggg cacgttcgag atcacgatcg gctcgcggaa
     3721 gatcacgccg ccgaggatgt tcctgatcgt cccgttcggc gagcggtaca tctccttcag
     3781 gccgaattcc tcgacccggg cctcgtccgg agtgatggtc gcgcacttga cgccgacgcc
     3841 gtgttccttg atcgcgttgg ccgcatcgac cgttatctga tcgtcagtgg cgtcccggct
     3901 ctcgatcccg aggtcgtagt acttcagatc cacgtcaagg tagggaagga tcagctgttc
     3961 cttgatgaac gaccagatga tgcgggtcat ctcatcgcca tccagctcga cgatcgggtt
     4021 ggtcactttg atcttcgaca cgacaggtac ttcccttcga tttcggcgta aggctcttgc
     4081 aggaaccgat gctatagagc ggaaccggtt gaagtcgtca atgggtagtt ccccgagcct
     4141 cgaccagccg ggcctgtgag accattagct catgccggaa accgccgaaa ctccgcagca
     4201 cgccacccac accgtcttca atcagtccac cccgctggag aacgtcaatc tctatgagat
     4261 cgacctgcct ctccgggagg ccgtgcggcg cgagggagcc gactgggccg aggacaggat
     4321 ccgcgctctg ggggagatct gcggccagcc tgacacgctt ctggccggac gggacgcgaa
     4381 cgagttcccg ccgcgcctga aggccttcga ccgcttcggc aaccggatcg acgaggtgga
     4441 attccatccg gcctggcacc agttgatgtc gctcggcatc gaacacggac tgcacgccct
     4501 gccctggcgg gagccgggac aaggcgcgaa tgtcgcccgc gccgccctgt tctcccagct
     4561 gacccaggtc gaggcggggg tcggctgccc gatctcgatg accttctcgg tcatcccctc
     4621 gcttcgcaac cagccggatg tcgccgagga atgggagccg cgcttcacct cgctcgagta
     4681 cgacggcagg ttgcggccgc cggaggagaa gccgggctgc ctggccggca tggcgatgac
     4741 agagaaacag ggcgggtcgg acgttcgtgc gaacacgacc cgcgctgtcg ccctcaatgg
     4801 cggcggaccc
//
LOCUS       DAOTIP010000055         4788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_21133_length_4788_cov_3.440101, whole genome shotgun sequence.
ACCESSION   DAOTIP010000055 DAOTIP010000000
VERSION     DAOTIP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21133_length_4788_cov_3.440101"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="P5138_01725"
     CDS             complement(<1..749)
                     /locus_tag="P5138_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRV59325.1"
                     /translation="MTQLPHSIEAEEQLLGAMLVYEPAVSQAIEGVGLRPEDFYYRDR
                     HGLIFAAIADLYGAGTPCDTLTVTDALPAEKLKEIGGADFVSSLAEKVNAPGNASYHA
                     EIVQRHALARKAADWSDSIGKAVAEGDLDLAGELATKIPRPESDSIGRSRPRLYSIAD
                     LASLPEPRYLVQDFIRAEGTNALFGPSGSGKSFLALDWNLCVAAGLPFYGQAVEQAPV
                     VYVAGEGLAGAYNRIEAWCQSRGVEEPSDFH"
     gene            1942..2157
                     /locus_tag="P5138_01730"
     CDS             1942..2157
                     /locus_tag="P5138_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59326.1"
                     /translation="MSAEHDEIMRALKAIQDSAEMAAANSGEDPMTSEMRNAFGDLRA
                     GLDEALKRLDNIENHLGIVPNDELEDE"
     gene            2154..2393
                     /locus_tag="P5138_01735"
     CDS             2154..2393
                     /locus_tag="P5138_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59327.1"
                     /translation="MMAKAQSKIQMVGGRTYSLEITPEEVDAEIVNVLGGESNGVVDG
                     WRFICVKDYRGDSLRLNIDHIEAIEETRTGKATFS"
     gene            complement(2597..2947)
                     /locus_tag="P5138_01740"
     CDS             complement(2597..2947)
                     /locus_tag="P5138_01740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRV59328.1"
                     /translation="MEDFPKRGNIYWVEFDPTVGNEQAGRRPAVVVSHNVGNEHSGVV
                     TVIPFSTSIPRRRYPQDVHFKANEALPEPGVAKCAQIRTVSKKRVKALYGWVRPAKMA
                     DINAAISYVLALDD"
     gene            complement(3203..3496)
                     /locus_tag="P5138_01745"
     CDS             complement(3203..3496)
                     /locus_tag="P5138_01745"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRV59329.1"
                     /translation="MDENLEEDGQSAPEIVIRPEEMAGSWANYAVVSHSEHEFTLDFV
                     RLETDHRRGIVVSRVSVSPLFVTQLIDALQANWKSYAEKALPREIKDEPSDND"
     gene            complement(3640..3713)
                     /locus_tag="P5138_01750"
     tRNA            complement(3640..3713)
                     /locus_tag="P5138_01750"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3677..3679),aa:Met,seq:cat)
     gene            complement(3841..>4788)
                     /locus_tag="P5138_01755"
     CDS             complement(3841..>4788)
                     /locus_tag="P5138_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59330.1"
                     /translation="DGTLSWFSPLGWAQQTKPFWENHWWALLPPLAATGITLGLAFWQ
                     LARRDIGSGLIQPRPGPPAASPSLLHPLGFSLWLQRGTIIGWSSMLFLYGFAIGVMGN
                     NIEDILKSSTAFTEAFASASGDLVDSYFASILTVIAIMAAGFTISSALRPHSEESRDR
                     VAILLAAPLGKVRWVAGHVLIAYVASAMIMALTGLGLGLGLGVATGDFSETGTLLGSG
                     FAQVPAMWLTGSLAVLLFGISSRLSSLAWAFLAAFVLIWTVASFGELPQWIVDLSPFS
                     HVPAVPAVSLDVEPLLVMTLLAAVFTATGLALWRRRDLQ"
BASE COUNT          928 a         1400 c         1563 g          897 t
ORIGIN      
        1 atatgaaaat cactgggttc ctcgactcct cttgactggc accaagcttc gatgcggttg
       61 taggccccgg cgaggccttc accggccaca tagacgaccg gtgcctgctc gactgcctga
      121 ccgtagaagg gcaggccagc ggcgacacat aggttccagt cgagggcaag gaagctcttt
      181 ccgctgccgc tgggcccgaa gagcgcgttt gtgccttcgg cgcgaatgaa gtcctggacc
      241 agatacctcg gctccggaag tgaagccagg tcggcgattg aatagaggcg tggccggctg
      301 cggccgatcg agtctgactc cggcctgggg atcttggtgg caagttcgcc ggccaagtcc
      361 aggtcgccct ctgcgacggc tttgccgatc gagtccgacc agtcggcggc cttccgggcc
      421 aaggcgtgtc tctggacgat ctccgcgtgg taggaagcgt tacccggagc gttgaccttt
      481 tcggcgagcg aggatacgaa atcggccccg ccgatctcct tgagcttttc ggcgggaagc
      541 gcgtcggtga ccgtgagcgt gtcgcaaggt gtgcccgctc cgtagagatc ggcgatggcg
      601 gcaaagatga gaccgtggcg atcccggtag tagaagtctt ccggccttaa gccaactccc
      661 tcaatcgcct gactgacggc gggctcatag accagcatcg ccccgaggag ctgctcttcg
      721 gcctcgatgc tgtgaggcag ttgggtcact cggcgtctcc cacatcctcc agcgagctgg
      781 gggcgacgtt gagggctatc gccaaagcca agagggtgga gcgctgcggc cggtgtgatc
      841 gcctttcgat tcgtccgatc gtttccaggc tgaccccgga gagtgctgca aggtcggatc
      901 tcgatactcg ggcgcgagtc ctagccactt tcaacgggtg atcgtccgcg ggctttccta
      961 ctctgtctgt cgtgtcaggt gccgtcatgg gacatgaatc ttcgactcga attccgggga
     1021 accaggaagg ggtcaggtcg tgcgtgacaa accggcgtgc ctggcacgag aaatgcttgg
     1081 cgttttggct cccagaatgg gaaacgtcac ggatcgaagc gttggtctcg gatctcccct
     1141 cgacgctcca gggagtaccg acctacgcga tggggaacga gatcccggcg gctttcgacg
     1201 aaggtcttcg gctgcgactg ggaacttcag ttcacgactg gcacatttac tcgaagggcg
     1261 cgttccaact actggtcgag gtgtaccgcc gtgccggagg acaacttccc gatgtcgaag
     1321 ggttcgccgt ttgcgcggag gtaaccatgc cgatgaaatg gcttggcgct tggccaacca
     1381 cctacgagcg tctgagcgcc tacggcgctt gcgggaacgt tttcaagagg accaggcggg
     1441 accgctactt ctgcgactcc tgccggcctg ccgggaatcg gcccagccga tcaagcggta
     1501 tatcccccca cattgccgac cggcgcgttg agcggcacgg tggggagctg cgagttatct
     1561 ggctcggaca gtgccagtcc tgccgtgccg aatttgaggt tgagacctca accaaggcgc
     1621 gagcgccaga gctatgcgcg tcctgccgtt cgcccaagaa ccgccaaaga cggcgcagag
     1681 ggcaggagcc caaacgggct acctaccggt ttcactctgg agaccgattc agatggtcaa
     1741 cctttgaccg gagctttgtc gcccagggcg gtgaatcgga atggcttgag gtcccggaag
     1801 gctgcgctgg atatccgggg aagctgcgtt ttcaccgagg cggctacttc gagactgatg
     1861 acgccgagat cgccggtcgc atgcagagat tcgggctgat accagtctcg ttggacaaga
     1921 aatcgagata gggggctcac gatgagcgca gagcacgacg agataatgcg ggcactcaag
     1981 gccattcagg acagcgcaga gatggccgct gccaattctg gcgaagatcc gatgacttcc
     2041 gaaatgagaa acgcgttcgg ggacctccgg gccggcctcg atgaagcctt gaagcgccta
     2101 gacaacatcg agaaccacct gggcattgtg ccgaacgatg aactggagga cgaatgatgg
     2161 cgaaggcaca atccaagatc cagatggtcg ggggacggac ctactcgctt gaaatcacgc
     2221 cggaagaagt cgatgccgaa atagtcaacg ttctgggcgg agaatccaat ggtgttgtag
     2281 atggctggcg gttcatttgc gtgaaggact accgaggcga ttcgctgcgg ttgaacatcg
     2341 atcacatcga ggcgattgaa gagacccgga ctggaaaagc cactttcagc taatggcggt
     2401 ccacggtggc gcccctgacc ccgaccaggg caccggcatc ctcgcgggca cgatgcgggt
     2461 ttggcttctc caaagggtgg ctccgtggac ccaccctttc tccgggtgat gatgccgctc
     2521 caccttcggc catccgccgc ttcgcttctt tcgccagctc cggcagaaac tcaaccgcga
     2581 tcggtgtcag gggtggtcaa tcgtcgagag cgagcacgta ggagattgcc gcgttgatat
     2641 cggccatctt cgccggtcga acccacccat atagtgcttt cacccgcttc ttgctgactg
     2701 tgcgaatttg ggcacatttg gctaccccag gttcggggag tgcttcgttt gccttgaagt
     2761 ggacgtcttg ggggtagcgg cgtctcggaa tagacgtaga gaacggaatg actgtgacga
     2821 ctcccgaatg ctcgtttccc acgttgtggg acaccacgac agctgggcga cggcccgcct
     2881 gctcgtttcc gaccgttgga tcgaactcga cccagtagat gtttccccgc ttggggaagt
     2941 cctccacctt gggtggacgc ttcagtcgcc caaggtttcg agccctacca gcatgtgaga
     3001 ttcggcaaag gccgcgtctt cgtcaaagga ttcgcgatat ccctcgatgg caagtcggtc
     3061 acgtcgggcc ctgtccagag cctcataggc gttcgttcgg aatgccctga ctgcctgggt
     3121 agaggaagtc ccaggtgagt cgaatacccg atcgaggcca gcgtgcgtgg cgttggcggg
     3181 cgtctcgaat gcctcggtct tgtcagtcgt tgtcgctggg ctcatccttt atctcccgtg
     3241 gtagggcttt ctccgcgtag gatttccagt tcgcctgaag ggcgtcgatc aattgggtca
     3301 cgaacagcgg cgatacagac actctactga ccacgatacc tcgcctgtgg tcagtttcca
     3361 accgaacgaa gtcgagggtg aactcatgct cagagtggct gaccacggcg tagtttgccc
     3421 acgatcccgc catctcttcc ggtcggatca cgatttctgg agccgactgg ccatcctctt
     3481 cgaggttctc atccatgcgt agaagatatg cgctttcgga gatacccgtg ccgtgtcgtg
     3541 agccacgacg tgacactttc cgtgacaccc ccactgtttc cggggtatcg aggcgaccaa
     3601 gtgggacggg attcagcttt gtgagcgggt ttccagaggt accgggggag ggactcgaac
     3661 ccccgacacg cggattatga ttccgctgct ctaaccagct gagctacccc ggcaggggac
     3721 cggtgagttt agaggttgtg gccgggaccc ggttccctgc ctcgggtcgc ggttcgaggc
     3781 tgcctgcggg ccggctgggt tcaattcgcc aggactatgt acgagtgaat tcaacgcagg
     3841 tcattggagg tcgcggcggc gccagagggc gaggccggtt gcggtgaaga ccgcggccag
     3901 gagcgtcatc accaggagcg gctcgacgtc gagggagacc gccggaacgg ccgggacatg
     3961 ggagaagggt gaaaggtcga cgatccactg gggcagctcc ccgaaggagg ccacggtcca
     4021 gatcaagacg aaagccgcga ggaaagccca ggcgagcgag ctgagccggg aggagatccc
     4081 gaaaagaagc acggcgaggc ttccggtcag ccacatcgcc ggcacctggg cgaaacccga
     4141 tccgagcagg gtgccagtct cgctgaagtc accggtggcc acgcccaggc cgaggcccag
     4201 gccgagcccg gtcagggcca tgatcatcgc gcttgctacg tacgcgatca gcacatggcc
     4261 ggcgacccat ctcaccttcc ccagaggggc cgcgagcagg atcgcgaccc ggtcgcggct
     4321 ctcctcggag tggggccgga gggccgagct gatcgtgaag ccggccgcca tgatcgcgat
     4381 cacggtcaaa atcgaggcga aataggaatc gaccagatcg ccgctggcgg aagcgaaggc
     4441 ctcggtgaag gcggtactcg acttgaggat gtcctcgatg ttgttgccca tcacgccgat
     4501 cgcgaagccg tagaggaaaa gcatcgagga ccagccgatg atcgtccctc gctgcaacca
     4561 cagcgaaaag ccgagcgggt ggagcaggga aggcgaggcg gccggaggcc ccggccgggg
     4621 ctggatcagg ccgctgccga tgtcccgcct ggcgagttgc cagaaggcaa ggcccagggt
     4681 gatgcccgtt gcggccagcg gcggcagcaa ggcccaccag tggttctccc agaacggctt
     4741 ggtctgctgg gcccatccca gcggcgaaaa ccaggagagg gtgccgtc
//
LOCUS       DAOTIP010000056         4728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_21481_length_4728_cov_3.283330, whole genome shotgun sequence.
ACCESSION   DAOTIP010000056 DAOTIP010000000
VERSION     DAOTIP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21481_length_4728_cov_3.283330"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="P5138_01760"
     CDS             complement(<1..1842)
                     /locus_tag="P5138_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRV59331.1"
                     /translation="MNRVFVVLAAIAAVMLIGVAPATADPAPDLPAGPVLDKADWPND
                     PNFARCEIQDPVTGCQDSEQWNLYGPMDGLCLAPGGAVFDQPRPDGGLPCWARNATDP
                     ESSSGINMTGAWAQGNIGRDDVLIAYIEGGVNYSSNGIKDGLDAIYLNPGELPYPQGW
                     DGQDLGRYDFDGNGEFDIRDYAKDPRVNPDCPGATAQFTTYEEGTTRGCAEDGTHEYL
                     HSVNIAGPQTPYLSPDDLIAVFGHCKITDHRIESCPTDGRIDNDGNGYPNDVSGWNAY
                     QNTNDPQTEDRAYGHAPGLIGEMGGEANNGYAGVGNCRNCRVVPVKSGAESVGKADGL
                     APAITYATDLGATAMSSVVVSYAYSGALKAAVKHAVDKGVLMSMDSNDFDSMDHTDGH
                     LFDDVFPGNGLVQDVEGKDAQTFRARSNVTSYGTHSIFSGGETTTSGATPFQAAFLGM
                     VQSAALNAVDDPTNPYERKLTPNEVKQVLINTASEILPQRDTPDVLNQWPGNPDSKTD
                     ATHTAWSTQYGYGRVNIGKATKLIMDGRVPPTADINSPSWYKYVDPTKGGKLQIQGSV
                     APSAWGSEGLEWTLEWAVGANPGDDDFHTISTGKAAKSGVLGTLNLSD"
     gene            complement(1905..2957)
                     /locus_tag="P5138_01765"
     CDS             complement(1905..2957)
                     /locus_tag="P5138_01765"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRV59332.1"
                     /translation="MDADANGRPAEEETPSLAGRLVGAGTGGAKRVVGATGLDQAMET
                     AIEEALVRAIESEAVERSMTRIINGPLIENAVRDAMKSEAVEQALTEVVDSELVDRLW
                     ARILDSNETQRLIERIAEAPEVRAAIASQGLGLVDDIGRGIARVTRALDFVLERIARR
                     ILFRPRRAEPTDRVGIATRALGLGIDALIVNAVMITTTALVGLLASVVGLDTGSLSGA
                     AYAVGGIVWLGLGSLYLFTFWSLSGQTPGMRFLDIRIEHDGQRAIGPKRAFRRLVGFW
                     LAAIPFCLGFLGVLVRIDRRGLHDRFGRTMVYYVNPAGPNQPRLEPVINPMEEPSTEP
                     EAPEEPIPAEKPVSFN"
     gene            complement(2978..3682)
                     /locus_tag="P5138_01770"
     CDS             complement(2978..3682)
                     /locus_tag="P5138_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59333.1"
                     /translation="MGPALSRTLLEQVASGQRPATVRLSRPGRVVAFGRRDVVAPGYG
                     KAVEAAAGRGFPGMERISGGRAAAFTEGALSFTLTVPDPRPAEGTEQRFAEWSGLVRD
                     AFRDLGADARIGPVPGEYCPGEHSVNADDRIKLAGVGQRMVRGAAHIGFVILVSGSEL
                     VAETLEPVYAALDLEYNREAVGSLEDVIPGVSLEDAERALMKRLDDLARPEEVALDRH
                     TLETAAARATQFRSNP"
     gene            complement(3800..4006)
                     /locus_tag="P5138_01775"
     CDS             complement(3800..4006)
                     /locus_tag="P5138_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59334.1"
                     /translation="MLLGDLLRGFGPGTVDYVPDLKGDTDRGLRGDSGNGDFGTGGSG
                     AVLRPVPDQIPGDNQGGDQRQSND"
     gene            4028..4306
                     /locus_tag="P5138_01780"
     CDS             4028..4306
                     /locus_tag="P5138_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HRV59335.1"
                     /translation="MAQKKEADKFGDLPGSVTLATLNAIADCESGGDPEIVSSSGLYY
                     GKYQFSPGTWESVGGKGLPSEASEAEQDYRAALLYTRSGPGQWPVCGQ"
     gene            4335..>4728
                     /locus_tag="P5138_01785"
     CDS             4335..>4728
                     /locus_tag="P5138_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59336.1"
                     /translation="MILGAIEISLRSTRFSLSEVSSFSAERVVERSHGVTANVDGLDR
                     ITALLMSEVEFARDQGAERIEVTAVPALRGSRLVRLLDRVADAIGVGPIRIPTRRDWT
                     AATFLGVTIPAGDSLPDPVAVAVIGETAI"
BASE COUNT          729 a         1545 c         1596 g          858 t
ORIGIN      
        1 gtcgctcagg ttcagggtgc cgagaacgcc tgacttggcg gccttgccgg tcgagatcgt
       61 gtggaagtca tcgtcgccgg ggttggctcc gaccgcccat tcgagggtcc attcgaggcc
      121 ttcggatccc caggcggagg gtgccacgct gccctggatc tggagcttgc ctcccttggt
      181 cgggtcgacg tacttgtacc aggagggcga gttgatgtcg gcggtcggcg gaaccctgcc
      241 gtccatgatc agcttggtcg ccttgccgat gttcacccgc ccgtagccgt actgggtcga
      301 ccatgcggtg tgggtcgcat cggtcttcga gtcggggttg ccgggccact ggttgaggac
      361 gtcgggggtg tcgcgctgcg gcaggatctc gctggccgta ttgatcagca cctgcttgac
      421 ctcgttcggg gtcagcttgc gctcgtaggg gttggtcggg tcgtcgaccg cgttcagcgc
      481 ggcggactgg accatgccga ggaaagccgc ctggaacggg gtggcacccg aggtggtcgt
      541 ctcgccaccg gagaagatcg aatgggtgcc gtagctggtc acgttggagc gggcgcggaa
      601 ggtctgtgcg tccttgcctt cgacgtcctg gaccagtccg ttgccgggga acacatcgtc
      661 gaaaaggtga ccgtcggtgt gatccatcga gtcgaagtca ttcgagtcca tcgacatcag
      721 gacccccttg tcgaccgcgt gcttgaccgc cgccttgagg gcgcccgagt aggcgtagga
      781 aacgacgacc gaggacatcg cggtggctcc caggtcggtc gcgtaggtga tcgccggggc
      841 gagaccgtcc gccttgccga ccgactcggc gccggacttg accggcacca cacggcagtt
      901 gcggcagttg ccgacgccgg cgtaaccgtt gttggcttcg ccgcccatct cgccgatcag
      961 gccgggggcg tggccatagg cccggtcctc ggtctggggg tcattcgtgt tctggtaggc
     1021 attccagccc gacacgtcat tcgggtatcc gttgccgtcg ttgtcgatcc ggccgtcggt
     1081 cgggcaggac tcgatccggt ggtcggtgat cttgcagtgg ccgaagacgg cgatcaggtc
     1141 gtccggcgag aggtacgggg tctgcgggcc ggcgatgttc accgagtgga ggtactcgtg
     1201 ggtgccgtcc tcggcacagc cgcgggtggt gccctcctcg taggtcgtga actgggcggt
     1261 cgccccggga cagtcggggt tgacccgtgg gtccttcgcg tagtcacgga tgtcgaactc
     1321 gccgttgccg tcgaagtcgt aacggccgag atcctggccg tcccatccct gcgggtaagg
     1381 gagctcgccc ggattcaggt agatcgcgtc gagaccgtcc ttgatcccgt tcgagctgta
     1441 gttgacgccg ccctcgatgt aggcgatcag gacgtcgtcc cggccgatgt tgccctgggc
     1501 ccaggccccg gtcatgttga tccccgagga gctttcgggg tcggtggcgt tgcgggccca
     1561 gcaggggagg ccgccgtcag ggcgaggctg atcaaagacg gcaccgccgg gggcgaggca
     1621 gaggccgtcc atcgggccgt agaggttcca ctgttcgctg tcctggcagc cggtgaccgg
     1681 gtcctggatc tcgcagcggg cgaagttcgg gtcgttcggc cagtcggcct tgtccagtac
     1741 cggtccggcg ggcagatcgg gtgccggatc ggcggttgcg ggggcgactc cgatcagcat
     1801 gacggccgcg atagcggcaa gcacgacaaa gacgcgattc acgaacaaga catttcctcc
     1861 ctcgaagctc atttccctgg ccagctcacc tcccgagcct gaccctagtt gaaacttacc
     1921 ggcttttcgg ccggaatcgg ttcttcaggc gcctcgggct cggtcgaagg ctcctccatt
     1981 ggattaataa ccggttcgag acggggttga ttcggtccgg ccggattcac gtaatagacc
     2041 atggtgcggc cgaaccggtc gtgaagtccc cgacggtcga tcctgaccag gaccccgaga
     2101 aagccgaggc agaacggaat cgcggccagc cagaatccga ccagtcgcct gaaggcccgt
     2161 ttcgggccga tcgccctttg gccatcgtgt tcgatcctga tgtcgaggaa gcgcatgccg
     2221 ggggtctggc cggaaagcga ccagaaggtg aacaggtaaa gggagccgag tcccagccag
     2281 acgattcccc cgaccgcgta ggcggcgccg ctgaggctcc cggtgtccag tccgaccacc
     2341 gatgccagca gcccgaccag ggcggtggtg gtgatcatca ctgcgttcac gatcagagcg
     2401 tcgattccca gaccgagggc gcgggtcgcg attccgaccc ggtcggtcgg ctcggcccgg
     2461 cggggccgga agaggatcct ccgcgcgatg cgttcgagca cgaagtcgag agccctggtc
     2521 acccgggcga tgccccgtcc gatgtcgtcg accagaccga gaccctgcga ggcaatcgcg
     2581 gccctcacct ccggcgcttc ggcgattcgc tcgatcagac gctgggtttc attgctgtcg
     2641 aggatccggg cccagagccg gtcgaccagc tccgagtcga caacttccgt cagagcctgc
     2701 tcgaccgctt cgcttttcat cgcgtcacgg accgcgttct cgatcagcgg gccgttgatg
     2761 atccgggtca tcgagcgttc gaccgcctcg ctttcgatcg cccgcacgag cgcctcttcg
     2821 atcgccgtct ccatcgcctg gtcgagaccg gtggcgccga ccacccgttt cgcgccgccg
     2881 gtgccggccc cgaccagcct cccggccagt gacggggttt cctcctcggc gggcctgccg
     2941 ttcgcatcgg cgtccatgac gtcaggttat tcgagggtca ggggtttgac cggaattggg
     3001 ttgcccgggc tgccgcggtc tcaagcgtgt gccggtcaag ggcgacctct tccggccgtg
     3061 caaggtcatc aaggcgtttc atcaatgcgc gctcggcgtc ctcgaggctg actccgggga
     3121 tcacgtcctc gagactgccg accgcctccc ggttgtattc gaggtcgagg gccgcgtaga
     3181 ccggctcaag ggtctccgcg accagttccg acccggagac gaggatcacg aaaccgatgt
     3241 gggcggcgcc cctgaccatc cgctgcccga ccccggccag cttgatccgg tcatcggcgt
     3301 tgacgctgtg ctcgccgggg cagtattcgc cgggaaccgg gccgatcctg gcgtcggctc
     3361 ccaggtcgcg gaaggcatcc cggaccagtc cggaccattc ggcaaaccgt tgttctgtcc
     3421 cttccgccgg acgcgggtcc ggaacggtca gggtgaaact cagggcgccc tcggtgaacg
     3481 ccgccgcccg cccgccggag attcgttcca tcccgggaaa tccccggcct gcggccgctt
     3541 caaccgcctt gccgtagccg ggggcaacca cgtcgcgacg gccgaaggca acgaccctgc
     3601 cggggcggct gaggcgcacc gtcgccgggc gctggccgga agcgacctgc tccagcaggg
     3661 tcctcgacag cgccggaccc atctccgggc gatccggaaa tccgtcgcgc agcagctgcc
     3721 acccggaaag cggcgtccga ccggctctgg tatgagtgtt tgctaacaaa aggaagaaac
     3781 catcctcaac acacgcacat caatcattgc tctggcgctg atcgccgccc tgattatcgc
     3841 cgggaatctg gtcaggcaca ggcctcagga cggctccgga acctccggtg ccgaaatcgc
     3901 cattgccgct gtcacctcgc agaccccgat cggtgtcgcc tttcagatcg gggacatagt
     3961 cgaccgttcc agggccgaag ccgcggagaa ggtcgccgag cagcaacagg ccgaactcga
     4021 acggaagctg gcgcagaaga aggaggccga caagttcggt gaccttcccg gctccgtcac
     4081 cctcgccacc ctcaatgcga tcgccgactg cgaatccggg ggcgatccgg agatcgtgtc
     4141 ctccagcggg ctctactacg gcaagtatca gttcagcccc ggcacctggg agtcggttgg
     4201 agggaagggc cttccgtccg aggcttccga agcggagcag gactaccgcg cggccctcct
     4261 ctacacccgt tcgggccccg gccagtggcc ggtctgcggc cagtaggccg cagagccttt
     4321 aggattgccc cgtcttgatc cttggggcaa ttgaaatctc gctccgctcc acccgcttct
     4381 ccctttcgga ggtctcgtcc ttctcggcgg agagggttgt cgagcgcagc cacggggtga
     4441 ctgccaacgt cgacggactc gaccggatca cggccctgct catgtccgag gtggaatttg
     4501 cccgtgacca gggagcggag cggatcgagg tgaccgcggt gcctgccctc cgcgggtccc
     4561 gtctggttcg tctgctcgat cgggtcgccg acgccatcgg agtcggaccg atccgcatcc
     4621 ccacacgccg ggactggact gccgcgacgt ttctcggtgt gaccattcca gccggtgact
     4681 cgcttcctga cccggtggcg gtcgctgtga tcggcgagac ggcgatcg
//
LOCUS       DAOTIP010000057         4666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_21856_length_4666_cov_3.570375, whole genome shotgun sequence.
ACCESSION   DAOTIP010000057 DAOTIP010000000
VERSION     DAOTIP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21856_length_4666_cov_3.570375"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="P5138_01790"
     CDS             <1..584
                     /locus_tag="P5138_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HRV59337.1"
                     /translation="TSSVTRSACAKMRRLAIAMDMDETAMSSFRYGSPQPNYDTYSMY
                     RNEILGSQTALKPMLNLYKLAQKRGMATFVITARYEPLSADPLVGTILGNADLCDPGF
                     SWAGACGLDVNNFNFTKVTRANLKNEGYTGLTDLYMRPPDSPSKGEVKNSERAEISLR
                     RGYRIIAMFGDQSSDLEKGFYERGFKYQSPVGD"
     gene            656..2026
                     /gene="lpdA"
                     /locus_tag="P5138_01795"
     CDS             656..2026
                     /gene="lpdA"
                     /locus_tag="P5138_01795"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRV59338.1"
                     /translation="MADENFDLIVVGAGPGGYVAAIRAAQLGKTTAVVESDKAGGRCL
                     NYACIPAKTMLHTAEVFDEAKNSAYIGVKVEGAELDWEALGQRRTDVSASLSGGVKGL
                     FDRNKIKFIEGEGSLTAEGEVKVGDATYGAEKIILATGSTAQAIPGVEFSDRVLDTWA
                     AWSLPEQPKKIAVVGAGASGSEIASAYIRFGTEVILVEMLDQILPAEDKDVVRVVERT
                     FKKQGIEISTGTPVENVEVGSDSVKFTYGENSAEVDYLVIAGGRKPDIDGLGLDAAGV
                     KLDENGRVQVDEFQKTSNEKIYAIGDLTRGAALAHKAMEEGIVAAESAAGADTHPVDL
                     DLVPGATFCQPQVASVGLTEAQAKEKGLDIKTGKLKLGGVGAGTVYDDKDGMVKLVVE
                     KEYGEIVGAHIVGDRACDMIAELVAVMALEGGIQELQRIMHPHPTISEAVLDAARAVD
                     KWATHA"
     gene            2008..2613
                     /locus_tag="P5138_01800"
     CDS             2008..2613
                     /locus_tag="P5138_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRV59339.1"
                     /translation="MGHPRVTGPAEFFFDLGSPYAWLSAERIDSLFEEAGLELPLWTP
                     VLLGGLFQRFDRGSWAKTELREGGISEIERRAREYGLPEPIWPDPWPDSYLFAMRVAT
                     WAEREGRLREFTLASFRRAFTEGIDQAVPDHVLDSAEAAGLDRAATERGAADPEVKGA
                     LRSATERAGDLGVTGIPSVVVGETVFWGDDRLADAVSAARP"
     gene            complement(2603..3628)
                     /locus_tag="P5138_01805"
     CDS             complement(2603..3628)
                     /locus_tag="P5138_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59340.1"
                     /translation="MRNFKGLLAALTVALIGVAGIAQTASAAGPPLPVAKNGNKVKVF
                     ATGVAVPTQITFRRKTAFISGGAEDKIKGGLYYVSPGSSKAKRVPGTPPVAFGVVWHA
                     HRLYVNLGTEIRVLRGWTGKKFRHSRTLVSFNPKNFNYFTGLEFGPNGKLYTGVGLQF
                     DHKASKRPYSNSVVAINPRSGKIRTVSTGLRQPWMLTFLKGMSSPLVTVLGQDQPKNT
                     QAPDLIVKATPGADFGFPSCNWSNFKSCRKMGFKDPVLTLGPADPSPSPMGITHKGGK
                     LFVALFNGMTAKTGPVGPEVITTNVKGSVIRDFVTGFVAPLVLAHYQGGYLYMGDLTG
                     QVYRVKA"
     gene            complement(3753..>4666)
                     /locus_tag="P5138_01810"
     CDS             complement(3753..>4666)
                     /locus_tag="P5138_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59341.1"
                     /translation="SGPAISLTSPVTATNATHPQFSFDTTSTDVKTIGCLIVRKGDDA
                     DYSPCSPNQWFAADTPDEGDWQFAIRATDNVGNITQVVHDFVVDRTAPDAQFTSGPAD
                     GSVIKVGNVAYEWNVSDGLPVSQVCSWDNGEEVACDGSASRGLAAGTHSFKVTVSDEA
                     GNSTSISRSVTAKKDGDTTDPDPDPDTSDRTAPVVKIVAPRQTLRSARKALRLKVRCD
                     EACSGKVTVTGAGGIRFGGRVVLNGAGVAKLKLRPTAKVRRKLNRLAMRGRRGAEPRF
                     FNLVAKAGLKDAAGNTGKASLRFKVKA"
BASE COUNT          829 a         1491 c         1546 g          800 t
ORIGIN      
        1 ggacctccag cgtcacccgc agcgcctgcg ccaagatgag gcgcctcgcg atcgcgatgg
       61 acatggacga gaccgcgatg tcgagcttcc gctatggctc gccgcagccg aactacgaca
      121 cctactccat gtaccgcaac gagatcctcg gctcgcagac cgccctgaag ccgatgctca
      181 acctttacaa gctggcccag aagcggggca tggcgacctt cgtgatcacc gcccgctacg
      241 agccgctctc ggcggacccg ctggtcggca cgatcctcgg caacgccgac ctctgtgatc
      301 cgggcttctc gtgggcggga gcctgcggac tggacgtgaa caacttcaac ttcacgaagg
      361 tcacccgggc gaacctgaag aacgagggct acaccggcct gaccgacctc tacatgcgtc
      421 ccccggacag cccctcgaag ggtgaggtca agaactcgga gcgggccgag atctcgctgc
      481 gccgcggcta caggatcatc gccatgttcg gtgaccagag cagcgatctg gagaagggct
      541 tctacgagcg cggcttcaag taccagagcc ccgtcggcga ctaggacggg ctcacaacat
      601 cagccggtga aggcgggctg ccggggcacc tgactccggt agtctcgcct tcaccatggc
      661 tgatgaaaac tttgacctaa tcgttgttgg cgccggaccg ggcgggtatg tggccgcgat
      721 ccgcgccgcc cagctcggca agaccaccgc tgtggtcgag tcggacaagg ccggtggccg
      781 ttgcctgaac tatgcgtgca ttccggccaa gacgatgctc cataccgccg aggtgttcga
      841 cgaggcgaag aacagcgcct acatcggggt caaggtggag ggtgcggaac tggactggga
      901 ggccctcggc cagcgacgca ccgacgtttc ggcaagcctc tccggcggtg tcaagggtct
      961 tttcgaccgg aacaagatca agttcatcga aggcgaaggt agtctcaccg ccgagggcga
     1021 ggtcaaggtc ggcgacgcca cctacggcgc cgaaaaaatc atcctcgcga ccggttcgac
     1081 cgcccaggcg atcccgggcg ttgagttctc cgaccgggtg cttgacactt gggcggcctg
     1141 gtctttgcct gagcagccga agaagatcgc cgtggtcggg gccggtgcct ccggttccga
     1201 gatcgcttcc gcctacatac gcttcggcac cgaggtgatc ctggtcgaga tgctggacca
     1261 gatcctgccg gccgaagaca aggacgtcgt ccgggtggtt gaacgcactt tcaagaagca
     1321 gggcatcgag atctcgacgg gcaccccggt cgagaacgtc gaggtcggta gtgactcggt
     1381 caagttcacc tacggtgaga actcggccga ggttgactac ctcgtgatcg cagggggccg
     1441 caagcccgac atcgacggac tcgggctcga cgccgcgggc gtgaagctgg acgagaacgg
     1501 ccgggttcag gttgacgagt tccagaagac ctcgaacgag aagatctacg cgatcggcga
     1561 cctgacccgt ggggcagcgc tcgcccacaa ggcgatggaa gaagggatcg tcgcggccga
     1621 gtcggccgcc ggcgcagaca cccacccggt cgatctcgac ctggtcccgg gcgccacctt
     1681 ctgccagccg caggtcgcca gcgtcggcct taccgaggcg caggcgaagg agaaaggcct
     1741 cgacatcaag accggcaagc tgaagctcgg cggagtcggg gccggcaccg tctatgacga
     1801 caaggacggc atggtcaagc tggtggtgga gaaggaatac ggcgaaatcg tcggtgccca
     1861 catcgtcggt gaccgggcct gcgacatgat cgccgagctg gttgccgtga tggcccttga
     1921 aggcggcatc caggaactcc agcggatcat gcacccccac cccaccatct ccgaagccgt
     1981 cctcgacgcc gcccgggccg tcgacaagtg ggccacccac gcgtaacagg cccggccgag
     2041 ttcttcttcg acctcggctc tccatacgcc tggctttcgg ccgagcggat tgactcgctc
     2101 ttcgaggagg ccgggctgga gttgccgctc tggacgccgg ttctgctcgg cggactcttc
     2161 cagcggtttg atcgcgggtc ctgggcgaag acggagctga gagaaggcgg gatctccgag
     2221 attgaacgcc gggcccggga gtacgggctg ccggagccca tctggcccga tccctggccg
     2281 gacagctacc tcttcgcgat gcgggtcgcc acctgggctg aacgggaagg gcggctcagg
     2341 gaattcaccc tggcctcctt ccgccgggcc ttcaccgaag gaatcgacca ggcggtaccc
     2401 gaccatgtgc tcgactcggc cgaggccgcc ggtctcgacc gggctgccac cgagcggggc
     2461 gcagcggacc cggaagtcaa gggcgcgctg aggtcagcca ccgaacgagc cggtgacctc
     2521 ggggtcaccg gcatcccgtc ggtggtggtc ggcgagacgg tcttctgggg tgatgaccgc
     2581 ctcgccgatg ctgtgtcggc agctaggcct tgacccggta gacctgaccg gtcaggtcac
     2641 ccatgtacag gtaaccgccc tggtagtggg cgagcacgag cggcgccacg aacccggtga
     2701 cgaagtcccg gatgaccgag cccttcacgt tcgtggtgat gacctccggc ccgaccggtc
     2761 ccgtcttcgc ggtcatcccg ttgaatagcg ccacaaacag cttgccgccc ttatgtgtga
     2821 tccccatcgg tgaaggcgac gggtccgccg gaccaagggt caggacggga tccttgaagc
     2881 ccatcttccg gcagctcttg aaattggacc agttgcagct cgggaaaccg aagtctgcgc
     2941 ccggagtcgc cttgacgatc aggtccggag cctgggtgtt tttcggctgg tcctggccga
     3001 gaaccgtgac cagcggagaa ctcatgccct ttaggaaggt gagcatccag ggctgtcgga
     3061 ggccggtcga aacggtcctg atcttcccgc tgcgcgggtt gattgccacg accgaattcg
     3121 agtacggccg cttgctggcc ttgtggtcga actgcaggcc cacgccggta tagagcttgc
     3181 cgttcgggcc gaactcgaga ccggtgaagt agttgaagtt cttcgggttg aaggagacca
     3241 gcgtccgtga atgccggaac ttctttccgg tccagccgcg aagcaccctg atctcggtac
     3301 cgagattgac gtacaaccga tgggcgtgcc agacgacgcc gaaagccacg ggcggtgtgc
     3361 ccgggacacg cttggccttg gaagaaccag gtgacacgta gtagaggcct cccttgatct
     3421 tgtcctcggc tccgccggag atgaaggcgg tcttgcgcct gaaggtgatc tgggtcggca
     3481 ccgccacacc cgtggcgaac accttgacct tgtttccgtt cttggcgacc gggaggggcg
     3541 gtcccgcggc gctcgccgtc tgggcgatcc ctgcgactcc gatcagggcg accgtcaaag
     3601 ccgccagcaa acccttgaag ttcctcaatt gattctcact ttccctggtt ggctgcaacc
     3661 agtccacctt actcgccggg acagcccggc attacaaatt tccggggtcg gacacccgcc
     3721 agctcggcgg gcgcccgacc ccggctccgg tttcaggcct tgaccttgaa ccggagcgac
     3781 gccttgccgg tgtttccggc ggcatccttc agaccggcct tggcgaccag gttgaagaaa
     3841 cgaggctccg caccgcgacg gccgcgcatg gccagccggt tcagcttgcg acggaccttc
     3901 gcggtcggtc gcagcttcag cttcgcgaca ccggcgccgt tcagcacgac ccggccgcca
     3961 aagcggatcc cgccggcacc cgtgaccgtg acctttccgg agcaggcttc gtcgcagcgg
     4021 accttcagcc gcagcgcctt gcgggcggac ctcagcgtct gcctgggagc gacgatcttg
     4081 accaccggag cggtgcgatc ggacgtgtca ggatccgggt cgggatcggt cgtgtcgccg
     4141 tccttcttcg cggtgacgct cctggagatc gaggtcgagt tgccggcctc gtcggagacc
     4201 gtcaccttga agctgtgggt tccggcggca agtccgcggg aagccgagcc gtcgcaggcg
     4261 acttcctcgc cgttgtccca ggagcagacc tgggagacag gcagaccgtc gctgacattc
     4321 cactcgtagg cgacgttgcc gaccttgatc actgaaccat cggcaggtcc cgaagtgaac
     4381 tgcgcgtccg gggcggtccg gtcgacgacg aaatcgtgga ccacctgggt gatgttgccg
     4441 acgttgtcgg tcgcccggat cgcgaactgc cagtcccctt cgtcgggggt gtcagcggcg
     4501 aaccactggt tcggactaca ggggctgtag tcggcgtcat cgcccttcct gacgatcagg
     4561 caaccgatgg tcttgacgtc cgtggatgtg gtgtcgaaag agaactgcgg atgggttgcg
     4621 ttggtggcgg ttaccggcga ggtgagcgag atcgcgggac cggagt
//
LOCUS       DAOTIP010000058         4659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_21903_length_4659_cov_3.710686, whole genome shotgun sequence.
ACCESSION   DAOTIP010000058 DAOTIP010000000
VERSION     DAOTIP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21903_length_4659_cov_3.710686"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5138_01815"
     CDS             complement(<1..271)
                     /locus_tag="P5138_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV59342.1"
                     /translation="MSDTQVENMIIIGGGPAGYTAALYAARAELDPLVFEGFQWGGQL
                     QNTTDVENYPGFPEGIMGPEMMTRFRDQAERFGTRFVTDDVDKVEL"
     gene            complement(336..1457)
                     /locus_tag="P5138_01820"
     CDS             complement(336..1457)
                     /locus_tag="P5138_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRV59343.1"
                     /translation="MTEEGEVPGGEEEGDGLPSFTTKGAIQTVALIVLLILAIYFLLP
                     KLAGFGDAIGMLGEAKPVYVAIAIGFCVLSFITYIALFRAVVGGETFPITWNEAYQIN
                     MAGFAATLLFSAGGAGGVVLTYWALRQGGMERRDVGTRMVAFIVLHYLFYPLAVLVFG
                     ILLGTGVLSGSTTVSLTFVPAAIAAVVMLLFGALALLPTDFERRMDHFAKRHSGVTFL
                     ARLATLPAMVADGTRAALRLVANPSRAGLAVVGAVGFWAANIGILWASFKAFGIAIPL
                     GVVVMGFFIGMVANLIPFVPAGVGAVDAGMIGTFVLFGFPEEQVFAAVLIYRLVAFWL
                     PIPPGVAAFFQLRNTVKRWKREELPVDRPPRPGLIEEIL"
     gene            complement(1454..2341)
                     /locus_tag="P5138_01825"
     CDS             complement(1454..2341)
                     /locus_tag="P5138_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRV59344.1"
                     /translation="MIVPHVRRQLKIPAAVTVASTVSAPLAIAVLWPRSRRRDLGLFA
                     AQMWAFAVSHELPYDNPKRLRERLYIEYPIRIDRWIGRGRLPNSRLQEWLHQGRYGND
                     LTKFAAWTHWFWFIQPYASIFWIAIRNKEKFPESARQLAATFDIGCAMYFALPTAPPW
                     WAAENGYIDPKIADEHINRVMLEYGEKLWGPAWTRIFGTLGSNPWAAMPSLHFATAVM
                     AAILLSDAGGKVEGTLGWAYALSLGFALVYLGEHYVTDLLAGAGVVLLARKGEPIARP
                     IVEMINRRLQRLEAIAAGG"
     gene            2520..3368
                     /locus_tag="P5138_01830"
     CDS             2520..3368
                     /locus_tag="P5138_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59345.1"
                     /translation="MSLFISVAGALFAFALNASPAMALSLNPDAASPGIKDSNTLHWI
                     LFIVIVAVIVVVNLAILRAARPRTRAVSPKPGKSISQVKLAIGLGVFALALFVVATIF
                     SDKSREIPAVNSVPVAGQIKGEPLEITATGSQWLWRFDYPNGAFSYRRLIVPAGVTVK
                     LDLVSTDVVHGWNVPELTGKAQAVPGSTSYLYFRADDVGTYTNRSSVFSGQGYDTMEI
                     EVDVVPPAAYEKAIEVLKSDIQRAQDKVELEFRISQKKSEVAAAHADKMEADINKADI
                     ESADSK"
     gene            3365..>4659
                     /locus_tag="P5138_01835"
     CDS             3365..>4659
                     /locus_tag="P5138_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRV59346.1"
                     /translation="MSRTTSYPQTMPPTRPELASDGPEARRTRWTELATSADHKDIGR
                     ILVAASFGSIFLAALELLLTRLQLAVPENVFLKGVTFDRLLSVYGETAIFLIALPLVI
                     GLLYYVVPLQIGSRTTAIPRVGQIGLWLFIFGGILLYGSFLFTPPEAGINPLPPFSDA
                     TFTPNNGVDVWIASTGLVTLGLLMVSIDLLATLHNLRAPGMAWRRAPLFTWAGAIVTW
                     LFMIIAPIFLAAISMLLIDRLYDGGFFAGDAGGSPILWQHLSYLFFTGVYMAVSITFL
                     AAIGEIVQTFTGRELPRKSVIWSFVTIAIVGTLAWTQNMLTAPIPSGWKYYGMLMALA
                     LIVPFGLIFYNLITTVSASDFHMRAPLRFALGALSLASIGLAAEIAQSTIAVATQLSE
                     TYDAWAATHFALIGFVVFGGFAAVTYWYPKMTGHQLNEDR"
BASE COUNT          854 a         1602 c         1408 g          795 t
ORIGIN      
        1 tcagctcgac cttgtccacg tcatcggtga cgaaacgggt tccgaaccgt tccgcctggt
       61 cccggaaccg ggtcatcatc tcgggaccca tgatgccctc ggggaagccg gggtagttct
      121 cgacgtcggt cgtgttctgg agctgcccgc cccactggaa accctcgaag acgagcgggt
      181 cgagctcggc ccgtgcggcg tagagcgcgg ccgtgtaccc ggccggtcct ccgccgatga
      241 tgatcatgtt ctcgacttgc gtgtcgctca tttcaaggtt cccgttttct ggtccgaatt
      301 cattacttta ctttctgaag taagtgtagc gatactcaga gaatctcctc gatcaggccc
      361 ggccggggcg gacggtctac cgggagttcc tcgcgtttcc accgcttgac cgtattgcga
      421 agctggaaga acgcggcgac acccggcgga atcggcagcc agaaggcaac cagccggtag
      481 atcagaactg ccgcgaagac ctgttcctcc ggaaagccga aaagaacgaa ggtgccgatc
      541 attcccgcat ccaccgcccc gaccccggcc ggaacgaagg ggatcaggtt ggcgaccatg
      601 ccgatgaaga agcccatcac gaccacacca aggggaatcg cgatgccgaa cgccttgaag
      661 gaagcccaga ggatgccgat gtttgccgcc cagaacccga ccgccccgac taccgccagc
      721 cctgcccggg acggattggc gaccagcctc agtgcggccc tggtgccgtc ggccaccatc
      781 gcgggcagcg tggccagccg ggcgaggaag gtgacccccg aatgccgctt ggcgaaatgg
      841 tccatccggc gctcgaagtc ggtcggcagc agcgccagcg ctccgaacag gagcatcacg
      901 acggcggcga tcgcggcggg aacgaaggtc agggaaacgg tggtggagcc gctgagcacc
      961 ccggtcccga gcaggatccc gaagaccagg accgcgaggg gatagaagag atagtggagc
     1021 acgatgaagg cgaccatcct ggtgccgacg tcgcgccgct ccatccctcc ctggcggaga
     1081 gcccagtaag tgagaacgac accccccgcc ccgccggccg agaacaggag ggtggccgca
     1141 aaaccggcca tgttgatctg gtaggcctcg ttccaggtga tcgggaaggt ctccccaccg
     1201 accacggccc ggaacagggc tatgtaagtg atgaaggaga ggacgcagaa accgatcgcg
     1261 atggcgacat agaccggctt cgcctcgcca agcatcccga tcgcgtcacc gaaccccgcc
     1321 agtttgggaa gcaggaagta gatcgcgagg atcaggagaa cgatcagggc gacggtctgg
     1381 atcgccccct tggtggtgaa ggacggaagg ccgtcgccct cttcttcccc gcccggaact
     1441 tccccctctt cggtcatccg ccggctgcga tcgcttccag tcgctggagc cggcggttga
     1501 tcatctccac gatcggccgg gcgatcggct cgcccttgcg ggccagcaac acgacaccgg
     1561 cgccagccag cagatcggtc acgtaatgct cgcccaggta cacgagggca aatccaagcg
     1621 agagcgcgta agcccagccg agcgtgccct cgaccttgcc gcccgcatcc gaaagcagga
     1681 tcgccgccat cacagccgtg gcaaagtgga gcgaaggcat tgccgcccag gggttcgagc
     1741 ccagggtccc gaagatcctg gtccaggccg ggccccagag cttctcgccg tactcgagca
     1801 tcacgcgatt gatgtgttcg tcggcgatct tgggatcgat atagccgttc tcggcagccc
     1861 accagggcgg cgcggtcggc aacgcgaagt acatggcgca gccgatgtcg aaggtcgcgg
     1921 cgagctgacg ggccgactcg gggaacttct ccttgttgcg gatcgcgatc cagaagatcg
     1981 acgcataggg ctggatgaac cagaaccagt gggtccaggc ggcgaatttg gtcaggtcgt
     2041 tgccgtagcg gccctgatgc agccactcct gcaggcgcga attgggcagc ctgccccggc
     2101 cgatccagcg gtcgatccgg atcggatatt cgatgtaaag ccgctcgcgc aaacgcttcg
     2161 gattgtcgta gggaagctcg tgactgaccg cgaacgccca catctgggcg gcgaaaagcc
     2221 cgaggtcacg acgccgggaa cgaggccaca ggactgcgat cgccaagggt gccgaaaccg
     2281 ttgaagcgac cgtgaccgca gccggaatct tcagctggcg gcggacgtgg ggaacgatca
     2341 ccgctgccgc cagcgcgcct acggctgcga ggcgaatggc ggtccgttta gacgaccgcc
     2401 ggggcttgaa cgagctcggc aatctcatcc cgagttggaa gcgtatgcga cggcgcggcg
     2461 cggcctccgc aactgcgatt agaatggccc gcgtcttgct gcctcgatcc agaaaaccag
     2521 tgtctctgtt catctctgtc gccggcgccc tcttcgcttt cgcgttgaac gcctccccgg
     2581 cgatggcgct ttccctgaat ccggacgctg catcgcccgg catcaaggac tcgaacaccc
     2641 tccactggat cctcttcatc gtgatcgttg ccgtgatcgt ggtggtcaac ctggccatcc
     2701 tgcgggcggc ccggccgcgc acccgcgccg tcagccccaa gcccggcaag agcatctccc
     2761 aggtgaaact tgcgatcggg ctgggcgtct tcgccctcgc cctcttcgtg gtcgcgacca
     2821 tcttctccga caagtcccgc gagatcccgg ccgtcaacag cgtcccggtc gccggacaga
     2881 tcaagggcga gccgcttgag atcaccgcca ccggctccca gtggctctgg cgcttcgact
     2941 atccgaacgg cgccttcagc taccgccggc tgatcgttcc cgccggggtc accgtgaagc
     3001 tggacctcgt ctccaccgac gtggtccacg gctggaacgt ccccgaactg accggcaagg
     3061 cccaggcggt accgggctcg accagttacc tgtacttccg ggccgatgac gtcggcacct
     3121 acacgaaccg atcctcggtg ttctcgggac agggctacga cacgatggag atcgaagtcg
     3181 acgtggtccc gcccgccgca tacgaaaagg caatcgaagt gctcaagagc gacatccaga
     3241 gggcccagga caaggtggaa cttgagttcc gcatctccca gaagaagtcc gaggtcgcgg
     3301 cggcacatgc cgacaaaatg gaagccgaca tcaataaagc cgacatcgaa agcgctgact
     3361 ccaaatgagc cgcacgacca gctatcccca gacgatgccg ccgacccggc ccgagctcgc
     3421 ctctgacggt cccgaggccc gtcggacccg ctggaccgag ctcgcaacca gcgccgacca
     3481 caaggacatc ggccggatcc tcgtcgccgc ttctttcgga tcgatcttcc tggccgctct
     3541 cgagctcctg ctcacccgtc tccagctcgc cgtgcccgag aacgtcttcc tcaagggagt
     3601 caccttcgac cggctgctct ccgtgtacgg agagacggcg attttcctga tcgccctgcc
     3661 gctggtcatt ggcctcctct actacgtggt cccgctccag atcggcagcc ggaccaccgc
     3721 catcccgcgg gtcgggcaga tcggcctctg gctgttcatc ttcggcggca tcctgctgta
     3781 cggctcattc ctcttcacgc cgccggaagc cggaatcaat ccgcttcctc ccttctccga
     3841 cgccaccttc accccgaaca acggcgtgga cgtctggatc gcctcgaccg ggctggtcac
     3901 gctcggattg ctgatggtct cgatcgacct cctggccacg ctccacaacc tccgggcgcc
     3961 cggcatggcc tggcgcagag cgccgctctt cacctgggcc ggagcgatcg tcacctggct
     4021 gttcatgatc atcgccccga tcttcctcgc ggcgatttcg atgctgctga tcgaccgcct
     4081 ctatgacggc ggcttcttcg ccggcgacgc gggcggctcg ccgattctgt ggcagcacct
     4141 cagctacctg ttcttcacag gcgtctacat ggccgtctcg atcaccttcc tcgctgcgat
     4201 cggggagata gtccagacct tcaccggacg agagctgccc cgcaagtcgg tgatctggtc
     4261 cttcgtcacg atcgccatcg tgggcaccct cgcctggacc cagaacatgc tgaccgcgcc
     4321 catcccgagc ggctggaagt actacggcat gctcatggcg ctggccttga tcgtcccctt
     4381 cggactgatc ttctacaacc tgatcaccac cgtaagcgcc agcgacttcc acatgagggc
     4441 cccgctccgt ttcgccctcg gcgccctttc gcttgcctcg atcgggctcg ccgccgagat
     4501 cgcccagtcg accatcgcgg tcgccacgca gctttccgag acctatgacg cctgggccgc
     4561 gacccacttc gcgctgatcg gcttcgtcgt cttcggcggc ttcgccgcgg tcacctactg
     4621 gtacccgaag atgaccggac accagctgaa cgaggaccg
//
LOCUS       DAOTIP010000059         4643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22003_length_4643_cov_3.789669, whole genome shotgun sequence.
ACCESSION   DAOTIP010000059 DAOTIP010000000
VERSION     DAOTIP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22003_length_4643_cov_3.789669"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="P5138_01840"
     CDS             complement(<1..260)
                     /locus_tag="P5138_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRV59347.1"
                     /translation="MVESDQEAYDRFYNVIANPILWFIQHYLWDLSNAPDIRKEEITA
                     WYEGYEVVNEDIANCVIRQIEDREDPLVMLHDYHLYTAPAKIR"
     gene            complement(270..512)
                     /locus_tag="P5138_01845"
     CDS             complement(270..512)
                     /locus_tag="P5138_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59348.1"
                     /translation="MDADEKPLIIVSNRGPAQFDRTEEGQRTISRGGGGLVTALSGLV
                     KDRHALWIASAMTPEDVAVTEENDGRPIDVNLNGVD"
     gene            complement(574..1764)
                     /locus_tag="P5138_01850"
     CDS             complement(574..1764)
                     /locus_tag="P5138_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRV59349.1"
                     /translation="MSKRLTALLLIALLGVFALSGCGSDSGSDQDQGSGSNQDTQKIA
                     TSDADPVVVDSNGSEFNPSKVYSETIDGVVSIRSVFGDVKNSPATAAIAGGAGFVLSK
                     DGEIVTNAHVISDGSGDNRKPADNVYVEFTSGDILDAEIVGFDPFADVGLLKVSTDEV
                     DMKPLVLADSDKVVVGQPIAVIGSPFGEDQTLTTGVVSQTGRSVMSLTDFQIEGAIQT
                     DASINPGNSGGPMLDGDGHVIGISQQMKSGSGSSDGVGFGVPANSIRHSVEMLRDGGE
                     PEYAYIGVSTQPLYPQLAEKLGIDADQGAIVAEVVKGGPADDAGIKGSDRELIFQGAT
                     YDVGGDVIVAVNDVPIEHAEDLGRMVGALKPGDTATLELIRDGEHKTIDVKLENRPTA
                     VTRP"
     gene            complement(1776..2798)
                     /gene="thiL"
                     /locus_tag="P5138_01855"
     CDS             complement(1776..2798)
                     /gene="thiL"
                     /locus_tag="P5138_01855"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRV59350.1"
                     /translation="MDLSEFELIGRITGKAGGADRFVTGIGDDAAVIRTRGRIVTSVD
                     TAVEGVHFQPGRPARENARKAIASAISDLAAMGVGEGEKEILVALGAPADTSGEYLEE
                     LADGVVEAAASFGAELAGGDVVSSPVLFLSVTVIAHVGEDVPLVGRGGAKPGDVVAVT
                     GPVGGAAGGLALLNGTEVPDLSDLSRQLLVDCQVNPEPRLGAAPVLARCGATSMIDVS
                     DGLSADLGHVAESSGVTIRLEAAEIPVVPGVPEVAAALGVPSLSFALTGGEDYVLALT
                     IPQDRFEEADSALIEATGFELLAVGAVESSEGGRGKVTIETADGIEPPAPEGFDHFSG
                     PDIGSR"
     gene            2823..3902
                     /locus_tag="P5138_01860"
     CDS             2823..3902
                     /locus_tag="P5138_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRV59351.1"
                     /translation="MSEEPTTSSGEESKPSLLHRLGHLAATVGKTGPKKKSTKIILQG
                     SVAFLIFAFLVFTVVSQWSELKDKGVEFDFIWLIPAVLMMLLYYIAGAGVWGLILRFL
                     GSPVPAMETQKIWAQPLLVRYIPGTVLFILARILMAEKAGVQRRVSTAGIVYEQAVSI
                     AGALTIATWFLIDHPDLQDQPARWLPLLVLPVMVCLLHPRIFGPVSKRLLHALGRESL
                     PQLMPFRGVVTIYLAYVVVWAIMGVGVFFAARAVHHLNLDDLAIVAASQMIGFLAAVV
                     SAVTPAGLGVRDAAFAWAVKVALPSDSFGVGAVIAIAVRATQTVVELIYVGGITVLTR
                     SDEGEIELAEEAAEEAARLQPDPGS"
     gene            complement(3899..>4643)
                     /locus_tag="P5138_01865"
     CDS             complement(3899..>4643)
                     /locus_tag="P5138_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV59352.1"
                     /translation="AETLGGLTIGVSPLEMAGAYSTLASGGIRHDPTAIEKVVFPDGE
                     VDRPSKAKGVRVLSEAEAYEVTKILHDNITSGTGTGAYTGCGGQAGKTGTTDNQIDAW
                     FVGYQPNLTAAVWYGYPESNDISTGSDGGGVPASIWNSFFTNAAVPCEETPVPTETMS
                     WGDFSGGYTVSPGTQSDYGPDSTGTTGTDGTDTTGTDTTGTGTVGPADAYAPGVGQEP
                     AGVGGGTGGGTGGSTGGSTGGSTGGVTPG"
BASE COUNT          720 a         1562 c         1519 g          842 t
ORIGIN      
        1 cggatcttcg ccggcgccgt gtagaggtgg taatcgtgaa gcatcaccag cgggtcttct
       61 cggtcctcga tctggcggat cacgcagttc gcaatgtcct cattcacgac ctcgtatccc
      121 tcataccagg cggtgatctc ttccttgcgg atgtccggcg cgttggagag gtcccacagg
      181 taatgctgga tgaaccagag gatcgggttg gcgatcacgt tgtagaagcg gtcgtaggcc
      241 tcctggtcgc tctcgaccat caacacccgt taatcgaccc cgttcaggtt gacgtcaata
      301 ggccggccgt cgttctcctc ggtcacggcc acgtcctccg gggtcatcgc cgatgcgatc
      361 cagagggcat gccggtcctt gaccaggccc gagagggcgg tgaccaggcc gccgccgccg
      421 cgagagatgg tccgctgacc ttcctcggtc cggtcgaact gggcgggccc ccggttggag
      481 acgatgatca gtggcttctc gtccgcgtcc acgggcacat gttaatcgcc agccggtttc
      541 ggaacccgaa gtgaccggtt ggacagcgca ggatcagggt cgggtgaccg cggtggggcg
      601 attctcgagc ttcacgtcga tcgtcttgtg ctccccgtcg cgaatcaatt ccagcgtggc
      661 cgtgtcaccc ggtttgagcg cgccgaccat gcggccgagg tcttcggcgt gctcgatcgg
      721 cacgtcgttg acggcgacga tcacgtcccc gcccacgtca taggtcgcac cctggaagat
      781 caactcccgg tcgctgccct tgatgccggc gtcgtcggcc gggccaccct tgaccacctc
      841 ggccacgatc gcgccctgat cggcgtcgat cccgagtttc tcggccaact ggggatagag
      901 cggctgggtg ctgaccccga tgtaggcgta ctcgggttca ccgccgtccc tgagcatctc
      961 caccgaatgc ctgatcgagt tggccggaac gccgaagccg actccgtcac tggatccgga
     1021 cccgctcttc atctgctggc tgatcccgat cacgtgcccg tctccgtcga gcatcgggcc
     1081 gccggagttg ccggggttga tcgaagcgtc ggtctggatc gctccctcga tctggaagtc
     1141 ggtaaggctc atcaccgagc ggccggtctg tgaaacgacg cctgtggtga gggtctggtc
     1201 ctccccgaac gggctgccga tcacggcgat cggctggccc accacgacct tgtcgctgtc
     1261 ggccaggacc agcggcttca tgtcgacttc atcggtcgag accttgagca ggccgacgtc
     1321 ggcgaagggg tcaaagccga cgatctcggc gtcgaggatg tcgccggaag tgaactcgac
     1381 atagacgttg tcggcgggct tgcggttgtc gccgctgccg tcgctgatca catgggcatt
     1441 ggtgacgatt tccccgtcct tgctgagcac gaagccggcc ccgccggcaa tggccgcggt
     1501 ggcgggcgag ttcttcacgt caccgaagac cgaccggatc gagaccacgc cgtcgatggt
     1561 ctccgagtac accttcgaag gattgaactc cgagccgttc gaatcgacca cgaccgggtc
     1621 ggcatccgaa gtcgcaatct tctgggtgtc ctggttgctg ccggagccct gatcctgatc
     1681 cgacccggaa tctgagccgc agccggacag ggcgaaaacc cccagcaggg caatgagaag
     1741 cagggccgtc aagcgcttcg acatgaggac tgaccctacc gggagccgat gtccgggcca
     1801 ctgaagtgat cgaagccctc cggggcaggc ggctcgatgc cgtcggcggt ctcgatcgtg
     1861 actttgcccc tgccaccttc ggaagattcc accgccccga cggcgagaag ctcgaatccc
     1921 gtcgcctcga tcagggcgct gtcggcctcc tcgaagcgat cctggggaat cgtcagggcg
     1981 agcacatagt cctcgccgcc ggtcagcgcg aaggagagcg agggcacacc gagtgcggcc
     2041 gccacctcgg gaacccccgg gacgaccggg atctccgcgg cttcgaggcg gatcgttacc
     2101 ccggaggatt cggcgacatg cccgaggtcg gccgacagcc cgtcactgac gtcgatcatc
     2161 gaagtcgcgc cgcagcgggc gaggaccggc gccgccccga gccggggttc gggattgacc
     2221 tggcagtcga ccagcagctg acgggagaga tcactgagat cagggacctc ggtcccgttc
     2281 agcagggcga gcccaccggc cgcgccaccg accggaccgg tcacggccac cacgtctccc
     2341 ggcttcgctc cgccccggcc gaccagggga acgtcctccc cgacgtgagc gatcaccgtg
     2401 acgctgagga agaggaccgg cgaggaaacg acatcgcctc cggcgagctc ggccccgaat
     2461 gaggcggccg cctcgaccac cccgtcagcc agttcttcga ggtattcacc ggatgtgtca
     2521 gcgggtgctc cgagtgcgac caggatctcc ttctcgccct cgccgacgcc cattgccgcg
     2581 aggtcggaaa tcgcgctggc gatggccttg cgggcattct ctcgggctgg ccggccaggc
     2641 tggaaatgaa ccccttccac cgccgtatcg accgaggtca cgatgcgtcc gcgggtccgg
     2701 atcacggccg cgtcgtcgcc gattccggtc acgaaccggt ctgcgccacc ggccttgccg
     2761 gtgatcctcc cgatcagttc gaactcgctg aggtccatgg ctctgacact attgcgcgtc
     2821 agatgagcga ggaaccgacc accagctccg gggaggaaag caagccaagc ctgctccacc
     2881 ggctcggaca cctcgctgcc accgtcggca agaccgggcc gaagaagaag tcgaccaaga
     2941 tcattctgca gggctcggtc gccttcctga tcttcgcctt cctcgtcttc accgtggtct
     3001 cccagtggtc ggaactgaag gacaagggcg tcgagttcga cttcatctgg ctgatcccgg
     3061 ccgtcctgat gatgctgctc tattacatcg ccggggccgg ggtctgggga ctgatcctgc
     3121 gcttcctcgg cagccccgtg ccggccatgg aaactcagaa gatctgggct cagcccctgc
     3181 tggtgcgtta tatcccgggg accgtcctgt tcatcctggc ccggatcctg atggccgaga
     3241 aggccggggt acagcgccgg gtcagcaccg ccgggatcgt ctacgagcag gcagtctcga
     3301 tcgccggtgc cctgacgatc gcgacctggt ttctgatcga ccatcccgat ctgcaggacc
     3361 agccggcccg ctggctaccc ctgctggtcc tgccggtgat ggtctgcctg ctccacccgc
     3421 ggatcttcgg gccggtctcg aagcgcctgc tccacgcgct tggccgcgag tcgctgcctc
     3481 agctgatgcc attccgcggc gtcgtgacca tctacctggc ctatgtcgtt gtctgggcga
     3541 tcatgggcgt cggcgtgttc ttcgccgccc gggcggtcca tcatctgaac ctcgacgacc
     3601 tggcgatcgt cgcggcctcg cagatgatcg gcttcctcgc tgctgtcgtt tcggcggtga
     3661 ccccggccgg actcggggtg agagacgccg ccttcgcctg ggcagtcaag gtcgccctgc
     3721 cctcggacag cttcggggtg ggagccgtca tcgcaatagc ggtccgggca acccagacag
     3781 tggtcgaact gatctacgtc ggcggtatca ccgtccttac ccgcagcgac gagggagaaa
     3841 ttgaactggc cgaggaagcc gccgaagagg cggcccggct ccagcccgac ccgggcagct
     3901 agcccggagt aacgccgccg gtcgaaccgc cggtggagcc gccggtgctg ccaccggttc
     3961 cgccgcccgt tccgccgccg acgccggccg gttcctgacc gactcccggt gcgtaggcat
     4021 cggccggacc gaccgtgccg gtgccggtcg tgtcggtccc ggtcgtgtcg gtgccgtcag
     4081 tcccggtcgt gccggtcgag tccgggccgt aatcgctctg ggtgccgggg gaaaccgtgt
     4141 agccgccact gaagtcgccc cagctcatgg tctcggtcgg gacgggagtt tcctcacagg
     4201 gcacggccgc gttggtgaag aagctgttcc agatgctggc gggaacaccg ccgccgtcgg
     4261 atccggtgct gatgtcattc gactccgggt agccgtacca gacggccgcg gtcagattcg
     4321 gctggtagcc gacgaaccag gcgtcgatct ggttgtcggt cgttccggtc ttgccggcct
     4381 gcccgccaca gcccgtgtag gcgccggtgc cggtgccgct ggtgatgttg tcgtggagga
     4441 tcttggtgac ttcgtaggct tccgcctcgc tgaggaccct cacgcccttg gccttgctgg
     4501 ggcggtcgac ctcgccgtcg gggaagacga ccttttcgat cgcggtcggg tcgtgacgga
     4561 tgccgccgct ggccagggtg gagtaggcac cggccatctc cagtggtgac acgccgatgg
     4621 tcagaccgcc gagggtctcg gcc
//
LOCUS       DAOTIP010000060         4639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22033_length_4639_cov_3.542757, whole genome shotgun sequence.
ACCESSION   DAOTIP010000060 DAOTIP010000000
VERSION     DAOTIP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22033_length_4639_cov_3.542757"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1027)
                     /locus_tag="P5138_01870"
     CDS             complement(188..1027)
                     /locus_tag="P5138_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRV59353.1"
                     /translation="MNQLPADPALDADWTGICRRIVEEQKKLFEEYETIEKRDNYEGV
                     GEGGDHTLVLDRMCEDVIFAELEDLAENGAAAFTAISEERGTVEFNEGGNAWVVIDPI
                     DGSLNFKRTIPQHSLSIAVATAPNMGAVEFGYVYDFGTGDEFLAQSGEGAFLNEEKLN
                     LEPRDGLEVVGVEGSEPARLVPMLEQLAGKVYRVRCVGSLAISIASVAAGRFDGLAFP
                     HLSRSVDVAAAQLIAREAGALVDLGEGGLSEVGLALEDRFPVTVAASGEGLDTLVAAR
                     PVA"
     gene            1145..4219
                     /locus_tag="P5138_01875"
     CDS             1145..4219
                     /locus_tag="P5138_01875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRV59354.1"
                     /translation="MSDPESRPGADGDPGPGRDLVRGHGSGALLIRGGAGTGKSTAVR
                     ERVIHLSRPDTSEDDPARSALDPSRVAVIASTDRAATAHRRSLEESLPGPYEALSVFT
                     WEGLAEEILRHRPIEAGLGPSFEVIGAAERLAMLLSRVDELPLRHHEIRGNPAGLLRE
                     LLIGIDRAKREGTDGGELAELIQIHNRILAAADLLDRNDLPRIATELLADPAIAGEAI
                     GRFPHLVIDELEDLHPARAGLLERLAAAGPESVVAAIDPSRAIYGPGAEQRFLSAFPD
                     PAQFTLDEAWRMSEVRIKAARAALGSGPLPEGAWDARGDEGSVRYWRPASARAEAQAA
                     AREVEHQIASGTPAEELAIAVPDVNSQGVAIVGALAERGIPARPGGGAALFRQPEVRD
                     TIAWLRALTDPTDARAVTRALMRPPTELRSLDLARLTQISKRRKLDMVSACEASLESP
                     QMAPEARQRIEGFLNLYRAASGALNTRRPDAYVRRLIERIGFRRQRLFAARPETAERL
                     LGLSRLAEIATDFSRRDPDASVREFTVFLEALADGGLEPVGGPDLPGVGAVPVVGLDM
                     LKGGEWTRIWVLGLERPFGITRTPGPGIGEDPTHPGGPETARVAIATALTSARDEVVL
                     SRSGDVEPDPVAAILEATGGLEEIHEEELFGPAEDVAATYRAMLGEVMEASWKAGSEL
                     NEPRLDTAIDVNRAVARYLELLKLAALAQRDDGSSDEEAIEAVNGLLAQIATPEQLAE
                     LDASTLDPYLLAQERERGMRQGLADSRSEPSLDAFLPRRGDDLRLSATDLDMYLTCPL
                     KYKFARVFGIPTPPTVNMRFGIVIHNVLQRFHELELDVGRPVPDGEARLMSLLEEGWR
                     RGGFGDSHDELQFLDRARDAMRAYWRSEEASESEPAFLERQFEFKVGPHFLRGRVDRV
                     DRKPDGEFEVIDYKTGQRIDSSRHGGDIQLALYRLGAREAWGIEPEAGSYYYVLEGEK
                     VEVQSSPDDRERVERTVLEVGEGVLGQDFEPRPSPAVCGWCDFRLVCPAAEA"
     gene            complement(4220..>4639)
                     /locus_tag="P5138_01880"
     CDS             complement(4220..>4639)
                     /locus_tag="P5138_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59355.1"
                     /translation="FGIIISIAIGGVILLVTRGDVEQEKSSEQIAREQAEKATTVLGD
                     DADSIAAGVELADLRTNLKVIGEWQRYGIKRVEPNNGGYTIETALPPGQASIRKVARL
                     CERILGGDSPLVDAPAGLLVFGTGPEPLSGKSCGQGP"
BASE COUNT          746 a         1609 c         1567 g          717 t
ORIGIN      
        1 acgaacacat gttctttttt cgtgtgacct ccgtcacgga cacggaatcc ccattaccaa
       61 gcggaaaacc cggctcactc aaggcgcaag cggcgttgca ggaaggattc tggtcgatca
      121 gatgttcgca tcggggcgaa cagttgcgcc cggaaagcga tggatctgtg gcgctcgaac
      181 gggcggttca ggcaaccggg cgggccgcaa ccagggtgtc gagaccctcg ccgctggcgg
      241 ccaccgtgac cggaaagcga tcctccaggg ccaggcccac ctcgctcagc ccgccctcgc
      301 cgaggtcgac cagggcgccg gcctcacggg cgatcaactg tgcggcagcg acatcaacgg
      361 aacgggaaag gtggggaaag gcgagaccgt cgaagcgacc ggcggcgacc gaggcgatcg
      421 aaatcgccag cgatccgaca cagcgcaccc ggtagacctt gcctgcaagc tgctccagca
      481 tcgggacgag acgggccggc tcggatccct cgaccccgac cacctcaagg ccgtcgcggg
      541 gttcgaggtt cagcttctcc tcgttcagga aggctccctc gccggactgg gcgagaaact
      601 catcgccggt gccgaagtca tagacgtagc cgaactcgac cgctcccatg ttcggtgcgg
      661 ttgccacggc gatcgagagt gaatgctgcg gaatggtgcg cttgaagttg agcgaaccgt
      721 cgatcgggtc gatcaccacc caggcgtttc cgccctcgtt gaactcgacc gtgccacgct
      781 cctccgagat cgccgtgaaa gcggcggcac cgttttcggc gaggtcctcg agctcggcga
      841 agatcacgtc ctcgcacatt cggtcgagca ccagggtgtg gtccccgccc tcgccgacgc
      901 cctcgtagtt gtcgcgcttc tcgatcgtct cgtactcctc gaagagcttc ttctgctcct
      961 cgacgatgcg acggcagatc cccgtccagt cggcgtcgag tgcggggtct gcgggcagtt
     1021 ggttcacccg cgacatccta gagacaagcc acgcggggca gtgggtcgga ttctcggcgc
     1081 aggtgccgga aatccgaccc actccggggt ggaggctgga tcctcgctgg ggtaacttgg
     1141 aggtgtgagc gatcccgaat ccagacccgg tgccgatggt gatcccggcc ccggccggga
     1201 tctggttcgc gggcacggct ccggcgcgct cctgatccgc ggtggtgccg gcaccggcaa
     1261 gagcaccgcc gtgcgggagc gggtgattca cctttcgcgt ccggatacca gcgaagacga
     1321 ccctgcccgc tccgcgcttg acccctcgcg ggtcgcggtg attgcctcga ccgaccgtgc
     1381 cgccaccgcc caccggcgga gcctcgagga atccctgccc ggtccctacg aggcgctttc
     1441 ggtcttcacc tgggagggac tggccgagga gatcctccgt caccggccga tcgaagccgg
     1501 gcttggtccc tcgttcgagg tgatcggggc ggccgaacgc ctcgccatgc tgctgtcccg
     1561 ggtcgacgag ctgcccctac ggcaccacga gatccgcggc aacccggccg gcctgctgcg
     1621 cgagctgctg atcgggatcg accgggcaaa gcgggaagga accgacggcg gcgagctggc
     1681 cgaactgatc cagatccaca accggatcct cgcggcggcc gacctcctcg accggaacga
     1741 cctgccccga atagccaccg agcttctcgc cgacccggcg atcgccggtg aggcgatcgg
     1801 ccgcttcccg catctggtga tcgacgagct cgaggatctg catcccgccc gggccgggct
     1861 gctcgaacgg ctggccgcgg ccggtcccga gagcgtggtc gcggcgatcg acccttcccg
     1921 ggcgatctac ggacccgggg ccgagcagcg cttcctcagc gccttccccg accccgccca
     1981 gttcacgctc gacgaggcct ggcgaatgag cgaggtgcgg atcaaggcgg cccgggcggc
     2041 gctcggcagc gggccgctcc cggagggcgc ctgggacgcc cgtggtgacg agggttcggt
     2101 ccgttactgg cggcccgctt cggcgagggc cgaggcccag gccgcggccc gcgaggtcga
     2161 gcatcagatt gcctccggga cgcccgccga agaactggcg atcgcggtgc cggatgtgaa
     2221 ctcccaggga gtggcgatcg tcggcgctct ggccgagcgc ggcattccgg cccggcccgg
     2281 aggtggtgcc gccctctttc gccagccgga ggtgcgggac acgatcgcct ggctgagggc
     2341 cctgaccgac ccgaccgacg cccgggcagt cacccgggcg ctgatgaggc cgccgaccga
     2401 actgcgctcg ctcgacctgg cccggctaac ccagatctcc aagcgccgca agctcgacat
     2461 ggtcagcgcc tgcgaggcct cgctcgaatc gccgcagatg gcgccggagg cccgccagcg
     2521 gatcgaaggc ttcctcaacc tctaccgggc ggcatcgggc gcgctcaaca cccggcggcc
     2581 ggatgcctat gtgaggcggc tgatcgagcg gatcggcttc cggcggcagc ggctcttcgc
     2641 ggcccggcct gaaacggccg agcggctgct cggcctgtcg cgcctggccg agatcgcgac
     2701 cgacttctcc cggcgggacc cggatgcgtc ggtccgggag ttcaccgtct tcctcgaggc
     2761 cctcgccgac ggcggacttg aaccggtcgg cggccctgac ctgcctggcg tgggtgcggt
     2821 gccggtggtg ggtctcgaca tgctgaaggg cggagagtgg acccggatct gggtgctcgg
     2881 cctggagcgg cctttcggaa tcacccgcac ccccggaccg ggcatcggcg aggacccgac
     2941 ccatcccggt ggaccggaga ccgcgagggt cgcgatcgca accgcgctca cctcggcccg
     3001 cgacgaggtc gttctctcgc gttccggtga cgtcgaaccc gacccggtcg ccgcgatcct
     3061 cgaagcgacc ggcggcctgg aagaaatcca cgaggaggaa ctcttcggcc cggccgagga
     3121 cgtcgccgcc acctaccggg cgatgctcgg cgaagtgatg gaggcctcgt ggaaggccgg
     3181 ctcggaactc aacgaaccaa ggctcgacac ggcgatcgac gtgaaccggg cggtagccag
     3241 gtacctggag ctgctcaagc tcgcggccct ggcccagcgc gacgacgggt cgagcgatga
     3301 ggaagcgatc gaggccgtca acggactgct cgcccagatc gcgactccgg agcagctggc
     3361 cgaactcgac gcctcgacgc tcgaccccta cctgctcgcc caggaacggg agcgcggcat
     3421 gcggcaggga ctggccgact cgcgcagcga accctcgctc gacgcctttc ttccccgccg
     3481 gggagatgac ctgcgactgt cggccaccga tctcgacatg tacctcacct gtccgctcaa
     3541 gtacaagttc gcccgggtct tcgggatacc gaccccgcca acggtgaaca tgcgtttcgg
     3601 catcgtcatc cacaacgtcc tccagcgctt ccacgaactg gaactggatg tgggacgccc
     3661 ggtcccggat ggcgaggccc ggctgatgag cctgctcgag gagggctggc ggcgcggcgg
     3721 cttcggcgac agccacgacg agctccagtt cctcgaccgg gcccgcgacg cgatgcgggc
     3781 ctactggagg agcgaggaag cctccgaatc cgaaccggcc ttcctggaac ggcagttcga
     3841 gttcaaggtc ggtccgcatt tcctgcgcgg ccgggtcgac cgggtcgacc gcaaaccgga
     3901 cggtgagttc gaggtgatcg actacaagac cggccagcgc atcgactcat cccgccacgg
     3961 cggtgacatc cagctcgccc tctacaggct tggtgcgagg gaggcctggg ggatcgaacc
     4021 ggaagccggc agctactact acgtgctcga gggagagaag gtcgaggtcc agtcctcgcc
     4081 ggacgatcgc gaacgggtcg aacggaccgt gctggaggtc ggcgaaggcg tgcttggcca
     4141 ggacttcgag ccccgtccct cgccggcggt ctgcggctgg tgcgacttca ggctggtctg
     4201 cccggccgcc gaagcctgat cagggtccct gtccgcagga cttgccgctg agcggctccg
     4261 gtccggtgcc gaaaaccaga agtccggccg gtgcgtcgac caacggtgaa tctccgccca
     4321 ggatcctctc gcagagccga gccaccttcc tgatcgaagc ctgccccggg ggcagggccg
     4381 tttcgattgt gtagccacca ttgttcggct cgacccgctt gatcccgtaa cgctgccatt
     4441 ccccgatgac cttgaggttg gttctcaggt cggcgagttc gacacccgcc gcgatcgaat
     4501 cggcgtcgtc gcccaggaca gtcgttgcct tctcggcctg ctcccttgcg atctgctcac
     4561 tggacttctc ctgttcgaca tcgccgcggg tgaccagcag gatcacgccg ccgatcgcga
     4621 tcgagatgat gatcccgaa
//
LOCUS       DAOTIP010000061         4597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22303_length_4597_cov_3.525539, whole genome shotgun sequence.
ACCESSION   DAOTIP010000061 DAOTIP010000000
VERSION     DAOTIP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22303_length_4597_cov_3.525539"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="P5138_01885"
     CDS             <1..309
                     /locus_tag="P5138_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59356.1"
                     /translation="EVEDLLVQPGTYFNPQTEVVVVVDDSASIDQEIFNMEDYEGADW
                     VRIADDVAVDEQHRDELLEHFQTHYHPGSGGSVSGTALEQGDDELDEDEEGQEIGRED
                     "
     gene            309..1193
                     /locus_tag="P5138_01890"
     CDS             309..1193
                     /locus_tag="P5138_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRV59357.1"
                     /translation="MGDRGPLTLLGVPIDSVGTAGGTELGPAALRELLGPEGFKDAGD
                     TSRQIRGLDRDKANGWLAFDDILKMTAEVRARVAELTAEGNVPVILGGCCTLLPAALA
                     GARDSLGEFGLAYFDGHLDLFTGQTSPTGEGADFPVAASLGIAPEPFLDAVGETPLVD
                     PGRIALVGARDQEELDLIAPFPKGLGISRIEYRDDLRHADLARVGKSIEAELASGDSK
                     FWLHLDVDILDGDSFPATDYLMPDGLSMTELKSLISPLASSPGLIGFNVTCYNPEKDE
                     DGKCGAALAGLLRSTLAT"
     gene            1293..1763
                     /gene="bcp"
                     /locus_tag="P5138_01895"
     CDS             1293..1763
                     /gene="bcp"
                     /locus_tag="P5138_01895"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRV59358.1"
                     /translation="MLEEGDKAPDFTLKDQHGEKVKLSDHRKELVVLYFYPKANTGGC
                     TTQACSIRDNSDEYAKAGAKVIGISPDEVKDISKFADKNDLEFTLVADLGHKVADKYG
                     TWVEKSMYGNKYMGVQRATFIIRGMKILRVFPKVQPKKHDGLVLKALAEINEED"
     gene            1764..2552
                     /locus_tag="P5138_01900"
     CDS             1764..2552
                     /locus_tag="P5138_01900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59359.1"
                     /translation="MSSWDRGSYEVTAEQLAPVAPVVADAVEPLSGQPALDLACGTGN
                     VALELARRGASVTGLDGAPRLLGVAAERTAAEDLEVDFVQGDLTDLPFADDTFEAVTS
                     VFGSIFTSSPGDTAAEIGRVLRPQGRFAFTSWVPEGLFQYLQDMARAMVAGKFGQAPP
                     EGEDAPFEWGDELAVRELFSESGIAVQTETLDLTIEDESPARLNDRWFDLHPIWLTMK
                     GVVGEEAYEELRRESLPVVEQANEADDGSFRCTLRYLLFEGSPV"
     gene            complement(2557..3585)
                     /locus_tag="P5138_01905"
     CDS             complement(2557..3585)
                     /locus_tag="P5138_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRV59360.1"
                     /translation="MEYLLLITSFAVILTGALLFTNAIEWLGHKLNLGQGAVGSILAA
                     VGTAMPETLIPIVAIIGGVSGSDEVAVGAIVGAPFLLATIAMALVGISALAYIKRRPQ
                     GKHLEVHVETLDRDLVFFMIFFSLALIVGVVDVPGWVEIAGGIVCLLAYLYYVVQTIR
                     NSGDAAESHEIDPLIMDRRMDRTGGPPNAIMVIQLLVGLGAMVGGAHLFVHELLNVAE
                     AVGVSAIILSLILAPLATELPEKANSFFWVRDGKDSLALGNITGAMVFQSTIPVAIGM
                     IFIDWSLDKFAILSMGLGLVGGIVAYISLRRNGQFNLPSIVIWGLLYLTFLAFVILSG
                     DTGHSSAH"
     gene            complement(3606..4226)
                     /locus_tag="P5138_01910"
     CDS             complement(3606..4226)
                     /locus_tag="P5138_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59361.1"
                     /translation="MERLRIPLLVTILLASLLAFAGCGSSDPDPSTVLDRALTRENLA
                     TFGNGPGGPVGGVVKVQALGYEDRVLDERRVGASASVLSDIREALGSDSGLRGLADDI
                     EYDGTEEVAGVETDHISGSLEVAGLAKALDDAGGKEVTKIAGVESGKGLEDSLAAADF
                     DLYAGEEDGTIRRLDLTLALDDPGNALPATRIRFSLTPRQSGTPQT"
     gene            4256..>4597
                     /locus_tag="P5138_01915"
     CDS             4256..>4597
                     /locus_tag="P5138_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HRV59362.1"
                     /translation="MPPRTILYTGKGGVGKTSVAAATARRAAAEGRRTVILSTDPAHS
                     LSDSLGVELGPDPVEVSPGLWGQEVIAEAEMRRNWDRVAAWMTGLLQSKGVDQVRAEE
                     LSVPPGLDELFS"
BASE COUNT          872 a         1503 c         1485 g          737 t
ORIGIN      
        1 gaggtcgagg acctgctggt ccagccgggc acctacttca acccccagac cgaagtcgtg
       61 gtcgtggtcg acgattccgc gtcgatcgac caggagatct tcaacatgga ggactacgaa
      121 ggtgcggact gggtgcggat cgccgacgac gtagcggttg acgagcagca ccgggacgaa
      181 ctgctcgagc atttccagac ccactaccac cctggaagtg gtggatcggt ttcgggtacc
      241 gcgctcgaac agggcgacga cgaactcgac gaggacgaag aaggccagga gatcgggcgc
      301 gaggactgat gggtgaccgc ggcccgctga cgctcctcgg ggtcccgatc gacagcgttg
      361 gcacggcggg cggtaccgaa ctcggcccgg cagccctgcg ggaactgctc ggcccggaag
      421 gtttcaagga cgccggcgac accagccggc agatccgcgg actcgaccgg gacaaagcca
      481 atggctggct cgccttcgac gacattctca agatgaccgc cgaggtccgg gcgcgcgtcg
      541 cagaactgac cgccgaagga aacgtgccgg tgatcctcgg tggctgctgc accctcctgc
      601 cggcggcact tgccggcgcg agagactcgc tcggcgagtt cggactggcc tatttcgacg
      661 ggcacctgga cctcttcacc gggcagacct cccccaccgg cgaaggcgcc gacttcccgg
      721 tcgcggcctc gctcgggatc gctcccgaac cgttcctgga tgcggtcggc gaaacgcccc
      781 tcgtggatcc gggtcggatc gccctggtcg gcgcccgtga ccaggaggaa ctcgacctga
      841 tcgccccctt tccgaagggc cttggcatca gccggatcga atatcgcgac gacctccgcc
      901 acgccgacct ggccagggtc ggcaagtcca tcgaagccga actggcgagt ggtgactcga
      961 agttctggct ccacctcgat gtcgacattc tcgacggcga ctccttcccg gcaacggatt
     1021 acctgatgcc ggacgggctg agcatgaccg aactgaagag cctgatcagc ccgctggcct
     1081 cctcccctgg cctgatcggg ttcaacgtca cctgttacaa cccggaaaag gacgaggacg
     1141 gcaaatgcgg cgctgccctc gccggcctgc tgcggtccac tctcgcaaca taaggatgtc
     1201 ggcgagctgc gccccgcctt caactgaaac ccgtccgatc acgcccttca tcgacacatg
     1261 accggactct gttgagcact aggatcagag ccatgctcga agaaggcgac aaggctcccg
     1321 atttcaccct gaaggaccag cacggagaga aggtcaaact ctccgaccat cgcaaggaac
     1381 tggtcgttct ctatttctac cccaaggcca ataccggtgg ctgcaccacc caggcctgct
     1441 cgatacgcga caacagcgac gagtacgcga aggccggggc caaagtgatc ggcatctcgc
     1501 cggatgaggt caaggacatc tcgaagtttg ccgacaagaa cgacctcgaa ttcacactgg
     1561 ttgccgacct cggacacaag gtggccgaca agtacggcac ctgggtggag aaatcgatgt
     1621 acgggaacaa gtacatgggc gtccagcggg cgaccttcat tattcgcggc atgaagatcc
     1681 tgcgggtctt tccgaaagtc cagccgaaga agcacgacgg cctggtgctc aaggcactgg
     1741 ctgaaatcaa cgaggaggac tgaatgtcga gctgggacag gggttcatac gaagtcaccg
     1801 cggagcagct cgcaccggta gcaccggtgg ttgccgatgc ggtcgagccc ctttcgggcc
     1861 agcccgccct cgacctcgcc tgcggcacag gcaacgtggc cctggagctg gcccgtcgcg
     1921 gagccagtgt gaccggactt gacggtgcac cacgcctgct cggggtagcc gccgaacgga
     1981 ctgccgcaga agacctggag gtcgacttcg tccagggtga tttgaccgac ctgcccttcg
     2041 cggacgacac attcgaggct gtcacctcgg tgttcgggtc gatcttcacc agctcgcccg
     2101 gggacaccgc cgcggagatc ggccgggtgc tgcggccgca gggcaggttc gcgttcactt
     2161 cctgggttcc ggaggggctt ttccagtacc tgcaggacat ggccagggcg atggtggcag
     2221 gcaagttcgg ccaggccccg cccgagggcg aagacgcgcc gttcgaatgg ggcgacgagc
     2281 tcgccgtccg tgagctcttc tcggagagcg gcatcgcagt gcagacggag acgctcgacc
     2341 tgacgatcga ggacgaatcc ccggccaggc tgaatgaccg ctggttcgac ctccacccga
     2401 tctggctgac catgaagggt gtggtcggag aagaggctta cgaagagctc cgccgggaaa
     2461 gcctgccggt ggtcgaacag gccaacgaag ccgacgacgg cagcttccgc tgcacgctca
     2521 ggtacctgct ctttgagggc agcccggtct gagccgctag tgagccgatg agtggccggt
     2581 gtcgccgctc aggatcacga aggccaggaa ggtcaagtag agcaggcccc agatcacgat
     2641 cgacggcagg ttgaactgtc cgttcctgcg cagcgagatg taggcgacga ttccgccgac
     2701 caggccgaga cccatcgaga ggatcgcgaa cttgtccagc gaccagtcga tgaagatcat
     2761 gccgatcgct accggaatgg tcgactggaa gaccatcgcg ccggtgatgt tgcccagcgc
     2821 cagggagtcc ttgccgtcac ggacccagaa gaacgagttc gccttttccg gcagttcggt
     2881 cgcaagcggg gcgagaatca gggaaaggat gatcgccgag acgcccaccg cctcggccac
     2941 gttcagcagt tcgtggacga agaggtgggc accgccgacc atcgctccga ggccgaccag
     3001 caactggatc accatgatcg cgttcggtgg accaccggtg cggtccatcc tgcggtccat
     3061 gatcagcggg tcgatctcat ggctttcggc agcgtctccg ctgttcctga tcgtctgaac
     3121 cacgtaatag aggtaggcca gcaggcagac gatgccaccg gcgatctcaa cccacccggg
     3181 cacgtcgacc acgccgacga tcagggcgag ggagaagaaa atcatgaaga agaccaggtc
     3241 ccggtccagc gtctccacgt gaacctcgag gtgtttgccc tggggcctgc gcttgatgta
     3301 ggcgagcgcg gaaatcccga ccagggccat ggcgatggtg gccagcagga acggggcgcc
     3361 gacgatcgcg ccgaccgcga cctcgtcgga gccgctgacc ccgccgatta tcgcgacgat
     3421 cgggatcagg gtctcgggca tcgcggtgcc gaccgcggcg aggatcgaac cgaccgcgcc
     3481 ctggcccagg ttcagcttgt gcccgagcca ctcgatcgcg ttggtgaaca gcagcgcgcc
     3541 ggtcaggatg accgcgaagc tggtgatcag gagcaggtat tccattgggt tctcttcggg
     3601 actctctagg tctgaggggt gcctgactgg cgcggcgtga ggctgaaccg tatccgggtg
     3661 gccggaagtg cattgcccgg gtcgtcaagc gcgagcgtca ggtccagcct cctgatcgtg
     3721 ccgtcctcct cgcccgcgta gaggtcgaag tccgcggccg ccagcgagtc ctccagcccc
     3781 ttgcccgact ccaccccggc gatcttcgtc acctctttgc ccccggcatc gtccagggcc
     3841 ttcgcgagcc cggcaacttc caggcttccc gaaatgtgat ccgtttcaac tccggcgact
     3901 tcctcggtcc cgtcatattc gatgtcatcg gccagacccc gcagaccgga atccgacccc
     3961 agcgcttccc gaatgtcgct caggaccgaa gcactcgcgc caaccctgcg ttcatcgagc
     4021 acccggtcct cgtaaccgag ggcctggacc ttgactactc caccgaccgg gcccccggga
     4081 ccgttgccga aggtggcgag gttctcgcga gtcagggccc ggtcgagcac cgtcgacggg
     4141 tccggatcgc tactgccgca gccggcgaaa gccagcaggg aagccagcag gatggtcacg
     4201 agaagtggga ttcgcagccg ttccaagtcg gggcaggcta tacgctcgct gcctgatgcc
     4261 accccgcacg atcctctaca cgggcaaagg aggcgtcggc aagacgagcg tcgcggctgc
     4321 cacggcacgc cgcgctgctg cggagggccg gcgcacggtg atcctctcga ccgaccccgc
     4381 ccacagcctt tccgactcgc tcggagtcga actcggtccc gatccggtcg aggtctcccc
     4441 cggcctctgg ggccaggaag tgatcgcgga agcggagatg cggcgcaact gggaccgggt
     4501 cgccgcctgg atgaccggac tccttcagag caagggcgtc gaccaggtgc gggccgagga
     4561 actgagcgtc ccgcccggcc tcgacgagct cttttcg
//
LOCUS       DAOTIP010000062         4587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22360_length_4587_cov_3.414387, whole genome shotgun sequence.
ACCESSION   DAOTIP010000062 DAOTIP010000000
VERSION     DAOTIP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22360_length_4587_cov_3.414387"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="P5138_01920"
     CDS             complement(<1..333)
                     /locus_tag="P5138_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRV59363.1"
                     /translation="MSDKRINDLEPATDKEAKARIAALDAAKSQIEKQFGAGSLMKLG
                     DQASVNVEAISTGALPLDIALGVGGIPRGRIVEIYGPESSGKTTLVYHIIAEAQKRGG
                     VCAFVDAEH"
     gene            complement(575..1894)
                     /locus_tag="P5138_01925"
     CDS             complement(575..1894)
                     /locus_tag="P5138_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRV59364.1"
                     /translation="MTLGDEIFRPGSSSDGEIRAGILVTGTEVLTATIRDENGPWLSQ
                     QLSDLGIELVEILVVADHPGDLASGLEHMKSVGIDLIVTTGGLGPTADDLTAEVVAAF
                     SGRKMELDREMEARIQAILARFAASTNLDLSTDALNEANRKQAMIPVGAVALNPVGTA
                     PGLIVPGQSEADPLVLVLPGPPRELKPMWETALKEPMLRQVLDRATRLRKYRLRMFGT
                     PESELAMSLREIEKDGLSFDGLEITTCLRKGEVEVDVRYRDAAEGSAEQLKEALKERH
                     SRTLYSLDGRTVDEVVSGLLEGRRLGLAESCSAGLLAARIADVPGSSDYFAGGVVSYS
                     NEAKRDLLGVDQALLDEFGAVSAEVAEAMATGALDRFGADVAVAITGIAGPGGGSEEK
                     PVGLVHFNVRTAEGGVRAAAPVIPGGRRDVRERSALVAMHLLREMLA"
     gene            complement(1956..>4587)
                     /locus_tag="P5138_01930"
     CDS             complement(1956..>4587)
                     /locus_tag="P5138_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRV59365.1"
                     /translation="RQHHLDLIGLGCVLLGIYLVFVLYFGWNGGRIGSGISDGLMYLF
                     GLVAYVAPVALFAAGAIAIFRPAVPTTRPLKTGAALMVAGLLLAFAGGTFGLGGGRPE
                     RIASFDPGWFPDHGGVVGESLYWVFASALQPFGAHLVAGFAILAGVLLLTGTTVATVL
                     GRGGNAIRKASTTSAEKTRTFARTAILHDGKGEEGDSGAETVVIAEPEPIPFGEEPTV
                     TIGSADSTDEMELPQTAEKEDSPFAVDIWAEEGGEPTVSDRTERLEPIEIFGEGGAHA
                     AIGEPGTAVAEPGSDDGTGGGDSGDDGVFSDDFEEELRQLEDEFDETPEEPAEEPEPA
                     ADVEADPETKEEQLQLTPQGEKRGATLSEEINYKAPPIKVLEKGQKGGGPDPAEQKEL
                     ARKLVETLGHFGVEAKVVGVVTGPHVSRFELQLAPGTKVKKVSELANDLAYALASTDI
                     RILAPIPGKQAVGVEVPNSSRNMVRLGDIYGKPKEKDSPLLAWLGKGIDGKPVSTDLA
                     KMPHVLVAGTTGSGKSGCVNAVLSSILMRSSPNEVRLVLVDPKQVELNHYETVPHLLT
                     PVVTNPKLAANVLNNLIAEMETRYGLMKEARARNIEDYNKAKAAEGEPRLPYILCVID
                     ELADLMMVAPADVEASIIRLAQKSRATGIHLLLATQRPSTDIITGTIKVNIPSRIAFA
                     VSSQTDSRVILDQGGAESLLGQGDMLFRGPGTSKLARIQGAFVTEDEIARITDFWSAQ
                     GEPEFEQELLSEPETVQEENSDGEFDPDQDDLLNEAIMLVVETETASVSMIQRRLRVG
                     YTRAGRLIDMLERRGVISGYEGSKPRQVLITHADLPRVLGGGNAGEEPEGDSPDVAEA
                     APEAAGDSDGTGGGTAGTTG"
BASE COUNT          718 a         1572 c         1455 g          842 t
ORIGIN      
        1 gtgctcggca tcgacgaagg cgcagacccc gccgcgcttc tgggcctcgg cgatgatgtg
       61 gtagacgagc gtcgtcttgc ccgaggactc cgggccgtag atctcgacaa tccggccccg
      121 gggaatcccg cccacgccga gggcgatgtc gagcggcagg gccccggtcg aaatcgcctc
      181 cacgttcacg gaggcctgat cgcccagctt catcagggag ccggcgccaa actgcttctc
      241 gatctggctc ttggccgcgt cgagggcggc gattctggcc ttggcttcct tgtcggtggc
      301 cggctccagg tcattgattc tcttgtcgct catctgattc acctcgccgc cgccctgtcg
      361 ggcagatcgt tggtcctgtc gtgcccgaac ggacacgaac acaagttcgt ctttggaaca
      421 gtaagtcctt cttcggacgg atcttttgga ataccgcttc aggacccgga atctgcctga
      481 aaacccctca aatgtgtgca tacggggcaa caactccgtc acgggtccgc cacgcaaagc
      541 aaacacccag ggtcggacgc ctttggagcc gaggtcaggc cagcatttcg cgaagcaggt
      601 gcatggcgac cagggccgac ctctccctca cgtcgcgccg gccgcccgga atcaccggtg
      661 ctgcggctct cactccgccc tcggcggttc gcacgttgaa gtggaccagt ccgaccggtt
      721 tctcctccga gccgccgccc ggcccggcga tcccggtgat cgccacggcc acgtcggcac
      781 cgaagcggtc cagggctccg gtcgccatcg cctcggccac ctccgccgac accgcgccga
      841 attcgtccag gagggcctga tcgacaccca gcaggtcccg tttggcctcg ttcgagtagc
      901 tgaccactcc gccggcgaag taatccgaag aacccggcac gtcggcgatc ctggccgcca
      961 gcaggccggc gctgcatgat tcggccaggc ccagtcgcct tccctccagc agcccggaaa
     1021 ccacttcgtc cacggtcctg ccatcaaggc tgtaaagggt gcgggaatgc cgctccttca
     1081 gggcctcctt cagctgttcg gccgacccct cggcggcatc ccggtagcgc acatccacct
     1141 cgacctcgcc cttccgcagg caggtggtta tctctagccc gtcgaaggaa agtccgtcct
     1201 tctcgatctc ccgcaggctc atcgccagct ccgactccgg ggtcccgaac atccgcaggc
     1261 gatacttccg cagccgggtc gcgcgatcga gcacctggcg cagcatcggc tccttcaggg
     1321 ccgtttccca catcggtttc agttcccggg gcggccccgg caggacgagc accagtggat
     1381 ccgcttcgga ttgaccggga acgatcagcc cgggcgcggt gccgaccggg ttgagggcca
     1441 cggccccgac cgggatcatc gcctgcttgc gattggcttc gttcagggcg tcggtcgaga
     1501 gatcaaggtt ggtgctggcg gcgaagcggg cgagtatcgc ctggatcctg gcctccatct
     1561 cccggtccag ctccatcttc cggccggaga aagcggcgac cacctccgcg gtcaggtcgt
     1621 ccgcggtcgg tccgaggccg ccggtggtaa cgatcaggtc gatcccgacc gacttcatgt
     1681 gctcaaggcc gctggcaagg tcaccgggat ggtctgcgac cacgaggatc tcgaccagtt
     1741 cgatcccgag gtcgctcaac tgctgcgaaa gccagggacc gttctcgtcg cggatggtcg
     1801 cggtcaggac ctcggtgccg gtgaccagga tgccggcccg tatctcgccg tccgaactgc
     1861 tgcccggcct gaatatttcg tcgccaagtg tcacggcctg accctacagc cgcgagccgg
     1921 ggttcagttc accgggatgt ttcccgcccg gtaattcagc cggtcgtccc ggcggtgccc
     1981 ccaccggtcc cgtccgaatc accggccgcc tccggtgctg cttcagccac gtcaggagaa
     2041 tcgccctcgg gctcttcgcc tgcgtttcca ccgcccagca ccctcggcaa atcggcatgc
     2101 gtgatcagga cctggcgggg tttcgagccc tcgtagccgg agatcacgcc tcggcgttca
     2161 agcatgtcga tcagcctccc ggcccgggtg tagccgaccc tgagccgacg ctggatcatc
     2221 gagacggaag cggtctcggt ttccaccacc agcatgatgg cctcgttcag caggtcatcc
     2281 tgatccggat cgaactcccc gtccgagttt tcttcctgaa ccgtctccgg ctcgctgagc
     2341 agttcctgtt cgaattccgg ctcgccctgg gccgaccaga aatcggtgat ccgggcgatt
     2401 tcgtcctcgg tcacgaaggc accctggatg cgggccagct tcgaggtgcc cggaccgcgg
     2461 aagagcatgt ctccctgacc cagcagtgac tcggcgccgc cctggtccag aatcacccgg
     2521 gaatcggtct gggaagaaac cgcaaaggcg atccgtgagg ggatgttgac cttgatcgtg
     2581 ccggtgatga tgtcggtcga aggacgctgc gtagcgagca gcaggtgaat gccggtggcc
     2641 cgcgacttct gggcaaggcg gatgatcgag gcctcgacat cggccggcgc gaccatcatc
     2701 aggtcggcga gctcgtcgat cacgcagagg atgtagggca ggcgcggctc gccctccgcg
     2761 gccttcgcct tgttgtaatc ctcgatgttc ctcgcccggg cttccttcat caggccgtag
     2821 cgggtctcca tctcggcgat caggttgttc agcacgttgg cggcaagctt gggattggtc
     2881 accaccgggg tcagcaggtg cgggaccgtc tcgtaatggt tcaactcgac ctgcttcggg
     2941 tcgaccagaa ccagtcggac ctcgttcggg gaggagcgca tcaggatcga agagagcacc
     3001 gcgttgacac agcccgactt gcccgagccg gtggtgccgg cgacgagcac atggggcatt
     3061 ttcgccagat ctgtcgagac cggcttgccg tcaatgccct tgcccagcca ggcgagcagc
     3121 ggggaatcct tctccttcgg cttgccgtag atatcgccga gacggaccat gttccggctc
     3181 gaattcggga cctcgactcc gaccgcctgc ttgcccggga tcggagccag gatgcggatg
     3241 tcggtggatg cgagcgcgta ggcgaggtcg ttggccagct cggaaacctt cttcaccttg
     3301 gtccccggcg cgagctggag ctcgaagcgg gacacgtgag gtccggtgac cacaccgacc
     3361 accttggcct cgactccgaa gtgcccgagc gtctcgacca gcttgcgggc gagttccttc
     3421 tgctcggccg ggtcgggtcc tccgcccttc tgccctttct ccaggacctt gatcggcggc
     3481 gccttgtagt tgatctcctc cgaaagcgtc gcccctctct tctcgccctg tggagtgagc
     3541 tgaagctgtt cttcctttgt ctcggggtcg gcttcgacgt cggcagccgg ttccggctct
     3601 tcggccggct cctccggggt ttcgtcgaat tcatcctcga gttgcctgag ttcttcctcg
     3661 aagtcgtcgc tgaagacccc gtcgtcaccg gaatcgccgc cgcccgtccc gtcgtcggac
     3721 ccgggctccg ccaccgccgt accgggctcc ccgatcgcgg catgggcgcc gccttctccg
     3781 aagatctcga tcggctcgag ccgctcggtg cggtcggaga cagtcggctc gccgccttct
     3841 tcggcccaga tgtcgactgc gaagggagag tcttccttct ccgcggtctg cggcagttcc
     3901 atctcgtcgg tcgagtctgc ggagccgatc gtcacggtcg gttcctcgcc aaagggaatc
     3961 ggttcgggct cggcgatgac gaccgtctct gccccggaat cgccttcctc tcccttgccg
     4021 tcgtggagga tcgcggtgcg cgcgaaagtg cgggtcttct cggccgaggt cgtactcgcc
     4081 ttcctgatcg cgttgccacc ccggccgagg acggttgcga cggtggtccc ggtcaggagc
     4141 agaactccgg ccagaatcgc gaagccggcc accagatgtg ccccgaaggg ctgcagcgcg
     4201 ctggcgaaga cccagtagag gctctcgccg acgacgccgc cgtggtcggg gaaccagccg
     4261 ggatcgaaag aggcgatacg ttccgggcga ccgccgccta ggccgaaggt gccaccggcg
     4321 aaggcgagca gcagcccggc caccatcagg gcagcgccgg tcttgagcgg gcgcgtcgtc
     4381 gggacggccg gcctgaagat cgcgatcgct ccggcggcga agagggcgac cggcgccacg
     4441 taggcgacca gtccgaagag gtacatcagt ccgtcgctga tcccggaacc gatccgcccc
     4501 ccgttccagc cgaagtagag gacgaatacg aggtagatcc cgagcaggac acagccgagg
     4561 ccgatcaggt ccaggtggtg ctggcgc
//
LOCUS       DAOTIP010000063         4580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22404_length_4580_cov_3.072486, whole genome shotgun sequence.
ACCESSION   DAOTIP010000063 DAOTIP010000000
VERSION     DAOTIP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22404_length_4580_cov_3.072486"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="P5138_01935"
     CDS             <1..323
                     /locus_tag="P5138_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59366.1"
                     /translation="LLHPQQMDQRAQKLHPARPAAEGFGAATLCGCTTRGWRRYPQRA
                     SNFQTRFSTPGVGAGSGVTNRVRAGWIVMPVSAGTRTFQLRYATTGGTAIFKNRVLRV
                     AVIR"
     gene            complement(327..536)
                     /locus_tag="P5138_01940"
     CDS             complement(327..536)
                     /locus_tag="P5138_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59367.1"
                     /translation="MAREVFDQARPRSTKAQRFADAFKGCDEWTIPPRKPRSMSSAVF
                     DAEESPFDSSVYGAEIIDLEFREAA"
     gene            1041..1262
                     /gene="rpmE"
                     /locus_tag="P5138_01945"
     CDS             1041..1262
                     /gene="rpmE"
                     /locus_tag="P5138_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRV59368.1"
                     /translation="MKADIHPEYTLANVHCSCGNQFYTRSTKSDLHVELCSACHPFYT
                     GKQKLVDTGGRVERFQRRAAKRAKAAAGN"
     gene            1299..2300
                     /locus_tag="P5138_01950"
     CDS             1299..2300
                     /locus_tag="P5138_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRV59369.1"
                     /translation="MEGGNTTPPNGVPTDVATPGAARPAPLEAKRDAPVGGQAVLEGV
                     MMRGVTTWAVSVRRPDSEIETTVEKIEPWARRHAVLRWPIIRGIVALAESLKIGFRAL
                     GMSANAQLEEGEEEQEIGGMAWGMTIFVSLAFAILLFFVIPVGLTSLIKDWLNSSFLF
                     WLVEGILRTAIFIGYIVMISRLEDLRRVFEYHGAEHKTISCYEAEDELVPSRATLYSR
                     LHPRCGTSFLLIVMVVAIFVFAPLGLPAWYWLVLSRIVGVPLIAGIAYELIKWAGRNR
                     DRSWVRAVMWPGLMLQNLTTREPDESQLEVAIAALDAVLEVEKPEENAYMEPIEIVA"
     gene            complement(2297..3877)
                     /locus_tag="P5138_01955"
     CDS             complement(2297..3877)
                     /locus_tag="P5138_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV59370.1"
                     /translation="MGYDYVVVGAGSAGCVLANRLSEDAGTSVLLLEAGGSNRHPSVV
                     IPAAFAEQFHTGRDWDLSTEPEPALHDRSLYVPRGKGLGGSSAMNAMLYVRGRPLDYD
                     LWEEGGCPGWGWKDVKPYFLKSEDNSRGESDDHSTGGPLRVEDPRSPRKLTKTLIESV
                     EALGYPFIDDYNGPEQDGVAWAQGTQRKGRRWGCNEAYLKPARKRSNLEVVTGATVVR
                     IETADGRATGVTYLTGKGATETTAEAGREVFLSAGALGSPQLLMLSGIGPADHLREHG
                     IEVRLDSPGVGQNLQDHPYLVCIWEAPGGDSLYAAEKPKALAEWAFRRTGPLTSTVAE
                     AFLFTRSRPGLPAADLQFHLVPGYFSQHGADKFAGDAVTMGPVLVSPKSRGEVTLKSS
                     DPGAKPSIVTNSMSDPEDLDAMVHGVKLARQIAGTEPLASKVSREIYPGAEAFSDEAI
                     EDDIRDRTELLYHPVGTCRMGSDDEAVLDPELKVKGIDGLRVVDASVMPLIPGGNTNA
                     PTIMVAEKAADLIRGSSS"
     gene            3926..>4580
                     /locus_tag="P5138_01960"
     CDS             3926..>4580
                     /locus_tag="P5138_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRV59371.1"
                     /translation="MEVVIEKLAEQIEARFTELEQQMSDPEVIGDRERYAEVGREYRQ
                     MEPANALVAEYRTLSDDLEGARELLEEGEDEELEKVVREAPARLEELEEEIRLAMVEP
                     DPNDDKNVIVEIRAGTGGDEAALFAGDLYKMLTRYAAERGFATEVLSQSAAEQGGFKE
                     VTFEVRGDGAYSIYKYEGGTHRVQRVPETESQGRIHTSTATVAVLPEAEEVEVDIDQN
                     "
BASE COUNT          782 a         1469 c         1534 g          795 t
ORIGIN      
        1 cactgcttca cccgcagcag atggaccagc gtgcgcagaa gcttcatccc gcgcgccccg
       61 ccgccgaagg attcggcgcc gccacgcttt gcgggtgcac gacgcgcgga tggcggcgat
      121 atccgcagcg ggccagcaac ttccagacca gattctccac cccgggagtc ggcgcgggga
      181 gtggtgtgac caaccgggtc agggccggct ggatcgtgat gccggtctct gcggggaccc
      241 ggaccttcca gctgcgctat gcgacaaccg gtggaacggc gatcttcaag aatcgcgtgc
      301 tcagggtggc tgtgatcaga taggtctcag gctgcttcgc ggaactccag gtcgatgatc
      361 tcggctccgt agacgctgga atcgaaaggt gattcctcgg catcgaagac ggcgctggac
      421 atcgatcggg gcttccgcgg cgggatggtc cactcgtcgc agcccttgaa agcatcggca
      481 aaacgctgcg ccttggtgct gcgggggcga gcctggtcga atacctctct ggccatacgg
      541 acgatcctcc tccggaaggg gttatggaaa gtgtgttcgc tccgtcggac ggatttcctg
      601 ccatttttct tgcaatttgc ggaaatccgg atttccgctc cccacggcac gttccaccct
      661 cgtgtctgct ggtcacgtcc cgggcaccgg gtcgaggggc ccggcacagg tatgtccgaa
      721 aagaccgtcg cgcttagcgc tcccccgaag gtcttttcgg acatacccgc accggacccc
      781 ccaagacctg gcagttcgta cacgggtcag ggcacggccg cggtgagcac tggccctccg
      841 atccccacgg ctacgcgaaa ccggggttat ggatgattcg gggctgcccg aacgttgccg
      901 atggttggtg ggttcgacgc gggcgtaccc gaaaaagtcc ttcgggggag cccgcagggc
      961 gacggatctt tttcgggtgc gcccgtggcg gaccccacca cagacttcaa cggcttgacc
     1021 tcttctctag aatgggatcc atgaaggcag atatccatcc cgaatacacg ctcgcgaacg
     1081 ttcattgttc gtgcggcaat cagttctaca cgcggtcgac caagtcggac ctgcatgtcg
     1141 agctttgctc tgcctgccat cccttctaca ccggcaagca gaagctggtc gacaccggcg
     1201 gacgggtcga gcgcttccag cggcgtgctg ccaagcgcgc caaggccgct gcgggcaact
     1261 agcccgcggc tgctcaacca gcccggaacc ttcagtccat ggagggcggc aacaccacgc
     1321 cgccgaacgg cgtccccacg gacgtcgcga ctccgggtgc cgcaagaccg gcaccgctcg
     1381 aggcgaagcg tgacgccccg gtaggcggcc aggctgtact cgaaggggtg atgatgcggg
     1441 gggtcaccac ctgggcggtt tcggtgcgcc ggccggacag cgagatcgaa accacggtcg
     1501 agaagatcga accatgggcc cgtcgtcatg cggtcctcag gtggccgatc atccggggca
     1561 tcgtggccct ggccgaatcg ctgaagatcg gcttccgggc gctcggcatg tccgccaacg
     1621 cgcagctcga ggaaggcgag gaagagcagg agattggcgg catggcctgg ggcatgacca
     1681 tcttcgtctc cctcgccttc gcgatcctgc tcttcttcgt gatcccggtc ggactgacca
     1741 gcctgatcaa ggactggctc aattcctcct tcctcttctg gctggtcgag gggatcctcc
     1801 ggaccgcgat cttcatcggc tacatcgtca tgatttcccg cctcgaggac ctgcgccggg
     1861 tcttcgagta ccacggagcg gagcacaaga cgatctcctg ttacgaggcc gaggacgaac
     1921 tggtcccctc gagggccacc ctctactcgc ggctccatcc ccgctgcggc accagcttcc
     1981 tgttgatcgt catggtcgtg gcgatcttcg tcttcgcccc gctgggtctg ccggcctggt
     2041 actggctggt tctttcccgc atcgtcgggg tgcccctgat cgccggcatc gcctacgaac
     2101 tgatcaagtg ggccggtcgc aaccgcgaca ggtcatgggt gcgggcggtg atgtggcccg
     2161 ggctgatgct ccagaacctg actacccggg agccggacga atcgcagctc gaagtggcaa
     2221 tcgcggctct cgacgccgtc ctcgaggtcg aaaagcccga ggagaacgca tacatggagc
     2281 cgatcgagat agtcgcctag gacgagcttc cgcggatcag gtcggcggcc ttttcggcga
     2341 ccatgatcgt cggggcgttg gtgttgcctc cggggatcag cggcatcacc gaagcgtcga
     2401 ccacccgcag accgtcgatg cccttcacct tcagttcggg atcaagcacc gcctcgtcgt
     2461 ccgagcccat ccggcaggtc ccgaccgggt ggtaaaggag ttctgtccgg tcccggatgt
     2521 cgtcctcgat cgcttcgtca ctgaaggctt ctgcgccggg gtagatctcg cggctgacct
     2581 ttgaggccag cggttcggtc ccggcgatct gccgggccag cttgactccg tggaccatcg
     2641 cgtcgagatc ctccgggtct gacatcgagt tggtcacgat cgagggcttg gcgcccgggt
     2701 cggagctctt cagggtgacc tcgcctcgtg atttcgggga gaccagaacc ggacccatgg
     2761 tcacggcgtc cccggcgaac ttgtcggcgc cgtgctggct gaagtagccg ggcacgaggt
     2821 ggaactggag gtcggcggct ggcagtccgg gccggctgcg ggtgaagagg aaggcttccg
     2881 ccaccgtaga ggtcaggggt ccggtccgtc tgaacgccca ctcagccagt gccttcggct
     2941 tctcggcggc atagagggag tcacctcccg gtgcctccca gatgcagacc aggtacgggt
     3001 ggtcctgaag gttctggccg accccgggcg agtcgagcct gacctcgatc ccgtgctcgc
     3061 gcaggtggtc ggccggaccg atgccggaga gcatcaggag ctgcggcgag cccagcgcgc
     3121 cggcggagag gaagacctcg cggccggcct ccgcggtggt ctcagtcgcg ccctttccgg
     3181 tcaggtaggt gactccggtg gctcgtccgt ccgcggtttc gatcctgaca accgtggccc
     3241 ccgttaccac ctcgaggttg cttcgtttcc gggccggctt caggtaggcc tcgttgcagc
     3301 cccagcgcct gcccttgcgc tgggtgccct gcgcccaggc gacaccgtcc tgttcggggc
     3361 cgttgtagtc gtcgatgaag ggatagccga gcgcctcgac cgattcgatc agggtctttg
     3421 tcaacttgcg tggcgaacgc ggatcctcga cccggagcgg cccgccggtc gaatggtcgt
     3481 ccgactcgcc gcgggagttg tcttcggact tgaggaagta gggcttgacg tctttccagc
     3541 cccagcccgg gcagccaccc tcctcccaga ggtcgtagtc cagcggccgg ccgcgcacgt
     3601 agagcatcgc gttcatggcg ctggaaccgc ccagaccctt gccgcggggc acatacagcg
     3661 agcggtcatg gagcgccggc tcgggctcgg tcgacaggtc ccagtcgcgg ccggtgtgga
     3721 actgctcggc gaaggcggcg gggatcacca cgctcgggtg acggtttgag ccgccggcct
     3781 ccagcagcag caccgaggtg cccgcgtctt cgctcaaccg gttggcaaga acacaaccgg
     3841 cagagcccgc tccgacgacc acatagtcat agcccatggc cggagaatat ccccgagggc
     3901 gcgtcggcca gggaggcccg tcagaatgga ggtcgtgatc gagaagcttg ccgaacagat
     3961 cgaggcgcga ttcaccgagc tcgaacagca gatgagcgac cccgaggtga tcggggaccg
     4021 cgagcgctac gccgaggtcg gccgcgagta ccggcagatg gagccggcca atgccctggt
     4081 cgccgaatac cggaccctga gtgacgacct ggaaggagcc cgggagctgc tcgaagaggg
     4141 cgaggacgaa gaactcgaga aggtggttcg ggaggccccg gcgaggctcg aggagctcga
     4201 ggaggaaatt cgtctggcga tggtcgagcc ggacccgaac gacgacaaga acgtgatcgt
     4261 cgagatcagg gccgggacgg gcggcgacga agcggcactc ttcgccggag acctctacaa
     4321 gatgctcacc cgttacgcgg cagagcgtgg ctttgcgacc gaagtgctct cccagtcggc
     4381 ggcggaacag ggagggttca aggaagtcac cttcgaggtc cgcggcgatg gcgcctattc
     4441 gatctacaag tacgaggggg ggacccaccg ggtgcagcgg gttcccgaaa ccgaatcgca
     4501 ggggcgcatc cacacctcga ccgcgaccgt ggcagtgctg cccgaggccg aagaggtcga
     4561 agttgacatt gaccagaacg
//
LOCUS       DAOTIP010000064         4551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22577_length_4551_cov_3.839190, whole genome shotgun sequence.
ACCESSION   DAOTIP010000064 DAOTIP010000000
VERSION     DAOTIP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22577_length_4551_cov_3.839190"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="P5138_01965"
     CDS             complement(<1..700)
                     /locus_tag="P5138_01965"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRV59372.1"
                     /translation="MAEVRSATTGDQPALTEPFEIGGVEISNRVLLAPLASIGNWFVR
                     LQAKRFGAGLAVSEMVSSFGLHYGNEKTMKELLRIHPDEHPVSIQLFGQSPEAMESGA
                     RIAAEAGADLIDINMGCPVPKVRKTGAGAELLSDPELALDLARAAGEGSGLPVTVKIR
                     SGLMPGDRSGYELSVRLVEEAGVAAIGFHPRVAKQGHKGQPDYAMARELTEAIDVPMI
                     ISGGLSSADAARRAY"
     gene            complement(693..1460)
                     /locus_tag="P5138_01970"
     CDS             complement(693..1460)
                     /locus_tag="P5138_01970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRV59373.1"
                     /translation="MLLAIDVGNTQTHLGAFEGTELTRHWRFQTRAGATSDELAERIS
                     GLMELRGTGFGDLDGLVVSSVVPPLGSEYTRLAEQYLDVDCLLVGPGVKTGMAIEIDT
                     PAEVGADRLVNAIAAYEEVGDVCVGVDFGTGINFDAVSADGAYLGGAIAPGVEISLSA
                     LTDRAARISRIDLNDPRKAIGTSSREAIQVGVLYGFAGLVDGIARRINRELGGQARFI
                     ATGGHANWIVPHSEEINEIDELLTLKGLRIIHERNRG"
     gene            1547..2305
                     /locus_tag="P5138_01975"
     CDS             1547..2305
                     /locus_tag="P5138_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRV59374.1"
                     /translation="MSKATLYSLPGSHPAKGVALMLDFKGIDYRTIDLMPLLHKGVVR
                     AAGFPGTTVPALKFNGEKIQNSVDIARALDRIVPEPPLLPADEEARKKVLMAEAFGEA
                     ELQHAVRQILWNAVRRQPRSIATFLEGSSLPLPQPVAAYTSGPIIWGEYKVNDSTDEN
                     VKSNLAALPEWLDRLDAWVEDGTLGGDTPNAADFQVMTSIGLLMTLQDLRPLVADRPI
                     GKAAMKVVPDYAGDVPPALPAEWLAPLRQQAAAV"
     gene            2344..3222
                     /locus_tag="P5138_01980"
     CDS             2344..3222
                     /locus_tag="P5138_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRV59375.1"
                     /translation="MSDFAKSVRLELGQDLSACTPGGGGDINRGWRIETAGGDRFFAK
                     SRPGGSVGEFANEAAGLAGLGEVGALPVPRVVAVVDDGTEPGLVLEWVEPGARGDEAG
                     LGRGLALIHRAGAAMFDQLAPGATGDEIRFGEAALPLNPTPADRGFGGVYSARLRYLA
                     ADAHARGSLDSPERETIERIADRAADLAGPIEPPSRVHGDLWSGNVLWSEGRPWLIDP
                     AAHGAHRELDLAMLELFGSPGQAFYDGYAEVSPLAEGFRDRIGFWQLQPLLVHAVLFG
                     GGYGRSAAAAAARYLD"
     gene            complement(3256..4347)
                     /locus_tag="P5138_01985"
     CDS             complement(3256..4347)
                     /locus_tag="P5138_01985"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HRV59376.1"
                     /translation="MSDSYDFAVRRDDMRDTTVIDIPGDDLEIEDGQILLAVDHFSLT
                     ANNVTYGAMGEAMSYWNFYPADDVSLGRIPAWGYADVIASKADGIEVGTRVFGYLPMS
                     THAVLEPGRIRESGFTDVTGYRTELPAVYNEYRLVTGEKDEERERLVSIFMPLYGTSW
                     LLADWLVEIDFAGAAQLVASSASSKTALGLGHALQTDFDSPIDTIGLTSPGNLEFTRG
                     TEHWDAVVTYDDLESIDTETPTIYVDFGGNANLRHRIHSHFGDGLVASVAVGIADWES
                     MVPEEGASLPGPQPQFFFAPDRVVKRNADWGEDGLGQRLARSQDRFTDRSREWMTVRT
                     GSGPDAIRDSLIELMDGKVPPNEAISVNP"
BASE COUNT          747 a         1517 c         1509 g          778 t
ORIGIN      
        1 cgtaggcgcg gcgggccgca tcggcgctgc tcaagccacc cgagatgatc atcggtacgt
       61 cgatcgcttc ggtgagttca cgggccatcg catagtccgg ctggcccttg tgaccctgct
      121 tggcgacccg cgggtgaaaa ccgatcgccg ccacgcccgc ttcctcgacc agccgcacgg
      181 agagctcgta gccggagcgg tcgcccggca tcagtccgga gcggatcttc acggtcaccg
      241 gcaggccgga cccttcgccg gccgcccggg cgaggtcgag agcgagctcc gggtcggaga
      301 gcagttccgc ccccgcgccg gtctttcgca ccttaggcac cgggcagccc atgttgatgt
      361 cgatcaggtc cgccccggcc tcggccgcga tcctcgcccc cgattccatc gcctccggcg
      421 actggccgaa cagctggatc gagaccggat gctcgtccgg gtggatccgg agcagttctt
      481 tcatggtctt ttcgttgccg tagtgaaggc cgaagctgga gaccatctcc gaaaccgcga
      541 ggccggcccc gaagcgcttt gcctgaaggc gcacgaacca gttgccgatg ctggccagcg
      601 gagccagcag cacccggttg gagatttcca ccccgccgat ctcgaatggc tcggtaagtg
      661 cgggctggtc tccggtcgtg gcagaccgga cctcagccac gattccgctc atgaatgatc
      721 cgcaacccct tgagcgtgag gagctcgtcg atctcgttga tctcttccga atggggcacg
      781 atccagttcg catgccctcc ggttgcgatg aacctggcct gccctccgag ttcgcgattg
      841 atccgacggg cgatcccgtc caccagtccg gcgaatccgt acaggacccc gacctggatc
      901 gcttcgcgcg atgaagtgcc gatcgccttc ctcggatcgt tgaggtcgat ccgtgagatc
      961 cgggcggccc tgtcggtgag ggcggaaagg gaaatctcga ctcccggggc gatcgcgccg
     1021 cccagatagg cgccatcggc cgagacggcg tcgaaattta tcccggtgcc gaagtccact
     1081 ccgacacaga cgtcgcccac ctcctcgtaa gcggcgatag cgttgaccag acggtcggcg
     1141 ccaacctcgg ccggagtgtc gatctcgatc gccatgccgg tcttgacccc gggaccgacc
     1201 aggaggcagt caacgtcgag gtactgctcg gccagcctcg tgtattcgga tccgaggggc
     1261 ggaacgaccg aggaaacgac cagtccgtcg agatcgccga agcccgtgcc gcgaagttcc
     1321 atcagcccgg agattcgctc cgccagctcg tcgctggtcg ccccggcccg cgtctggaat
     1381 cgccaatgcc tggtcagctc ggtgccttcg aaggccccga gatgggtctg ggtgttgccg
     1441 acgtcaatgg cgagaagcat ggacttaggg agtgtggcag gaagaccgcc gcaggggctt
     1501 ctcgcaaagc cacactttgt tccggcccgg tcatagactg cgggacatga gcaaggcgac
     1561 gctgtactca ttgcccggat cccatcccgc aaagggcgtt gccctgatgc tggacttcaa
     1621 gggcatcgac taccggacga tcgatctgat gcccctcctt cacaagggag tcgtgcgggc
     1681 cgccggtttc cccggtacga ccgttcccgc cctcaagttc aacggggaga agatccagaa
     1741 ctcggttgac atcgcccggg cgctggaccg gatcgtcccc gagccgccgc tccttcccgc
     1801 cgacgaggag gcccgcaaga aggtcctgat ggccgaggcc ttcggtgaag ctgagctgca
     1861 gcatgccgtg cgccagatcc tctggaatgc cgtccgacgc cagccccgct cgatcgccac
     1921 tttcctcgag ggatcgagtc tccccctgcc ccaaccggtt gccgcgtaca ccagcgggcc
     1981 gatcatctgg ggcgaataca aggtcaatga ctcgaccgat gagaacgtga agtcgaacct
     2041 cgcggcgttg ccggaatggc ttgaccggct cgatgcctgg gtcgaggatg gcactctcgg
     2101 cggcgatacc cccaacgcag ccgatttcca ggtcatgacc agcatcggat tgctgatgac
     2161 cctccaggac ctgcgcccgc ttgtagcgga caggccgatc ggcaaggcgg cgatgaaggt
     2221 tgttcccgac tacgccggtg acgttccccc ggcccttccg gccgagtggc tggccccgct
     2281 ccgccagcag gccgccgctg tctgacccac cgaaggaccg ctttctgtga cgtacaggcg
     2341 cagatgagcg acttcgccaa gtcggtccgg ctcgagctcg ggcaggatct gtccgcctgc
     2401 accccgggtg gtggcggtga catcaaccgg ggctggcgga tcgagacggc cggaggagac
     2461 cggttcttcg ccaagagccg cccgggggga tcggtcgggg agttcgcgaa cgaagcggcc
     2521 ggactggccg ggctgggaga ggtcggggcg ctaccggtgc cgcgggtggt ggcggtggtc
     2581 gatgacggga ccgaacccgg gctggttctc gaatgggtcg agccgggagc ccggggtgac
     2641 gaggcaggac ttggccgggg actggccctg attcaccggg ccggtgccgc gatgtttgac
     2701 cagttggcac ccggggcgac cggtgacgaa atccgcttcg gggaggcggc cctgccgctg
     2761 aatccgacac ctgccgatcg tggattcggc ggggtctatt ccgcgcggct ccggtaccta
     2821 gccgccgacg ctcacgcccg cggttcgctc gattctccgg agcgggaaac gatcgaacgg
     2881 atcgccgacc gggccgctga tctcgccggc ccgatcgagc ccccgtcgag ggtccacggt
     2941 gacctctggt ccgggaacgt cctctggagc gagggcagac cctggctgat cgatcccgct
     3001 gcccacggcg cccatcgcga actggacctg gccatgctcg aactgttcgg cagccccggg
     3061 caggccttct acgacggcta tgcggaagtc agcccgctgg ccgaaggctt ccgggaccgg
     3121 atcggcttct ggcagctgca gccgctgctg gtccacgcgg tccttttcgg aggcgggtac
     3181 ggacgcagcg ccgcggccgc ggccgcccgc tacctcgatt gaaccggccg gcgaagcccg
     3241 gcgatatctc aacggtcagg gattgaccga gatcgcctcg ttcggcggga cctttccgtc
     3301 catcaactcg atcagggaat cgcggatcgc gtcgggaccg gagccggtgc gcacggtcat
     3361 ccactcccgg ctgcggtcgg tgaagcgatc ctggctcctg gccaggcgct gtccgagacc
     3421 gtcctcgccc cagtctgcgt tccgcttcac gacccggtcg ggagcgaaga agaactgggg
     3481 ctgcgggccc ggcagggaag ccccctcctc cggcaccatc gattcccagt cggcgattcc
     3541 aaccgccaca ctggcaacca gcccgtcacc aaagtgactg tggatccggt gcctgagatt
     3601 cgcgttgccg ccgaagtcca cgtagatggt cggcgtctcc gtgtcgatcg attccaggtc
     3661 gtcgtaggtg acgaccgcgt cccagtgctc ggtgcccctg gtgaattcga ggtttcccgg
     3721 cgaggtcagg ccgatcgtgt cgatcggcga gtcgaagtcg gtctggaggg catgcccgag
     3781 cccgagggcg gtcttgctcg aggcgctgga tgcgacgagc tgggccgctc cggcgaagtc
     3841 gatctcgacc agccagtcgg cgagcagcca cgaggttccg tagagcggca tgaagatcga
     3901 taccaggcgc tcccgctcct cgtccttctc gccggtcacc aggcggtact cgttgtagac
     3961 ggccggcaac tcggtccggt agccggtcac gtcggtgaac ccggattccc ggattcgccc
     4021 gggttcgagc acggcgtggg tggacatcgg caagtagccg aacaccctgg tccccacttc
     4081 gatcccgtcg gccttcgatg cgatcacatc ggcgtacccc caggccggga tccggcccag
     4141 cgaaacatcg tcggccggat agaagttcca gtagctcatc gcctcgccca tcgccccgta
     4201 ggtgacgttg ttggcggtaa gcgagaagtg gtcgacggcg agcagtatct ggccgtcttc
     4261 gatctccagg tcgtctcccg gaatgtcaat gacggtggtg tcccgcatgt cgtcgcggcg
     4321 gactgcgaag tcgtatgagt cggacatgcc ggtgagccta ccgcgtccgg tgagacacat
     4381 gctttgagtg ttgcagcacc tgaacgaaag ctccccgcct tctggcccgt ggagtgcccg
     4441 gccgaccgat atgcgaacat atgttcgtgc gctgggacgg tcagagagtg actgcggacg
     4501 gacgccccgc cgaaacggga ggcgagccga ccctgcccgg catggaagac g
//
LOCUS       DAOTIP010000065         4548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22593_length_4548_cov_2.912531, whole genome shotgun sequence.
ACCESSION   DAOTIP010000065 DAOTIP010000000
VERSION     DAOTIP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22593_length_4548_cov_2.912531"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="P5138_01990"
     CDS             complement(<1..474)
                     /locus_tag="P5138_01990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRV59377.1"
                     /translation="MLTPKYARLLWRYLWRRVLTGTGRRWTTDGPIFFGRGLQLQTGK
                     RATIGFGRWVWIGDGTKIRCHEGAVEIGPKTVMGQECTISAYRKVKIGAECVIADRTM
                     FIDFDHGVVEVERPIRDQGIYMEDVIVGSNVWIGYGAAILRGVRVGDNSVIGTNSV"
     gene            545..1510
                     /gene="meaB"
                     /locus_tag="P5138_01995"
     CDS             545..1510
                     /gene="meaB"
                     /locus_tag="P5138_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRV59378.1"
                     /translation="MNDAATSLSPLAERLLGGDKRALARAITLIESDDPAGWDLVREV
                     YPKTGKARIIGFTGPPGSGKSTLIGALTKELRKEERQVAVLSIDPSSPFTQGALLGDR
                     IRLTEHFLDKGVFIRSMASRGALGGLSEAALQAALVMDASGKDDVLIETVGVGQGEID
                     IVDHADTIVLALMPGSGDSIQALKAGVMEIPDIIVVNKSDHPMTDTMIREVRGALSLS
                     HTPGGWRVPILRTEAARGEGIEELAAKVAEHRAFIEEQGTLAQRRARNLRSEVLGLAT
                     ARLRRRLEAAIEDDPDVVELLNRVVRRELDPASAAAELLEGEIDE"
     gene            1503..1904
                     /locus_tag="P5138_02000"
     CDS             1503..1904
                     /locus_tag="P5138_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59379.1"
                     /translation="MSENPSPQPSLEDVKAWDGYRLDDITGQGVARVEGLFVDGESGQ
                     PTWVLAKLGRFGKVVPVSIRECAAAAGRVWTPHDREVIRNAPAVDPSLPLNREQELQV
                     LDYYGIPETVGRGAELKDRPAGSNTANPLTA"
     gene            complement(1916..3064)
                     /locus_tag="P5138_02005"
     CDS             complement(1916..3064)
                     /locus_tag="P5138_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV59380.1"
                     /translation="MNFDLTDEQRLLRETVRDFAVSEVAPVAEELDRTKAFPYEIVEK
                     LGNLGLMGIPFPEEYGGSGGDILSYALAVEELARIDSSVAITMAAHTSLGTTPIYNWG
                     TEEQKAEWLPELTSGKRLAAFGLTEPEAGSDAGNTKTRAELDGDEWVVNGAKQFITNS
                     GTDISACVTITAVTGKDENGRAEISNIIVPNGTPGYSVDPPYRKMGWNASDTHPLTFE
                     DARVPESNLLGPRGQGFRQFLQILDGGRIGVAAMSVGVAQGALDEAIKYAKERQAFGQ
                     SISKFQAIQAKIADISAQLEAARLLTYRAAIEKDRGENFTLTAAQAKLITGRLAVRAT
                     EEAVQIHGGYGFIEEYPVCRFYRDAKILTIGEGTDEVQQMVIARALGC"
     gene            3468..3962
                     /locus_tag="P5138_02010"
     CDS             3468..3962
                     /locus_tag="P5138_02010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRV59381.1"
                     /translation="MPEISRSLTLEWQDPMAMASKGLELAGLDYMRAVMNQEVPPPPI
                     AVLMNMAPTEVEEGRAVFSALPGEEHYNPIGVVHGGFMATLLDSVAGCAVHTTLALGE
                     AYTTLTLEVKNLRPLTLDTGIVRAEGLVTYRGRRQATADAKLYAEETGKLLASASSTC
                     MIMS"
     gene            complement(3952..>4548)
                     /locus_tag="P5138_02015"
     CDS             complement(3952..>4548)
                     /locus_tag="P5138_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRV59382.1"
                     /translation="DAIAELDLPLDIIAVLPSTENMPSGTALKPGDIITQLNGKTVEV
                     TNTDAEGRLILADALVHCAREGADRMVDLATLTGAVLIGLGSTYAALISNDDGLAGEI
                     TEAAERSGELVWRLPLHPEYKELTKGEITDLVNASAQRKAGTIYAGSFLEEFTEGKPW
                     AHLDIAGTAWDTGREYWGKGPTGFGVHLLVALARDLSS"
BASE COUNT          775 a         1478 c         1521 g          774 t
ORIGIN      
        1 caccgagttg gtgccgatca ccgagttgtc cccgaccctg accccgcgga ggatcgcggc
       61 gccgtatccg atccagacgt tcgagccgac gatcacatcc tccatgtaga tgccctggtc
      121 gcggatcggc cgctcgacct caaccacgcc gtgatcgaag tcgatgaaca tggtccggtc
      181 ggcgatcacg cattcggcac cgatcttcac cttgcgatac gcggagatcg tgcattcctg
      241 gcccatcaca gtctttggcc cgatctcgac cgcgccctcg tggcagcgga tcttggtccc
      301 gtcgccgatc catacccagc gtccgaaccc gatcgtcgcc cgcttgccgg tctggagctg
      361 aagtccgcgg ccgaagaaga tcggcccgtc ggtcgtccag cgccggccgg taccggtcag
      421 gacccgacgc cagagatacc gccacaggag ccgcgcgtac ttgggcgtga gcattccctt
      481 gcggcggaaa gatgaaatcg agcgaatcaa accggccgtg actctaacta gactggcttc
      541 gttgatgaat gacgcggcga cctccctgag tcccctggcc gagcgtctcc tgggcggaga
      601 caagcgggcg ctggcccgcg ccataaccct gatcgagtcc gacgacccgg ccggctggga
      661 cctggtgcgc gaggtctacc cgaagacggg caaggccagg atcatcggtt tcaccggacc
      721 gcccggcagc ggcaagagca ccctgatcgg cgctttgacc aaagaactga ggaaggaaga
      781 acgccaggtc gcggtacttt cgatcgatcc ctcgagcccg ttcacccagg gcgccctgct
      841 gggcgaccgg atccgcctga ccgagcattt cctcgacaag ggagtcttca tccggtcgat
      901 ggcctcccgc ggagcacttg gcggcctttc cgaggcagcg cttcaggctg ccctcgtgat
      961 ggatgcctcc ggcaaagacg atgtcctgat cgaaacggtc ggggtggggc agggggagat
     1021 cgacatcgtc gatcacgccg acacgatcgt gctggccctg atgccgggct ccggagactc
     1081 gatccaggcc ctgaaggccg gggtgatgga gattccggac atcatcgtcg tcaacaagtc
     1141 cgaccatccg atgaccgaca cgatgatccg cgaggttcgc ggtgccctgt cgctctccca
     1201 cacgcccggt ggctggcggg tgccgatcct ccggaccgag gcggctcgtg gcgaggggat
     1261 cgaggaactc gcggccaagg tcgccgaaca ccgggccttc atcgaggaac agggaaccct
     1321 ggcgcagcgc cgggcccgga acctgcgctc cgaggtgctc ggcctggcca ccgcaaggct
     1381 gagaaggcgc ctcgaagccg caatcgagga tgatccggac gtggtggaac tcttgaaccg
     1441 agtggtcaga cgggaactgg acccggcttc ggccgcggcc gaactgctgg aaggagaaat
     1501 cgatgagtga gaacccatcc ccgcagccga gtctcgagga cgtgaaggcc tgggacggat
     1561 accggctcga tgacatcacc ggccagggcg tggcccgggt cgaagggctc ttcgttgacg
     1621 gggagtccgg acagccgacc tgggtgctgg ccaagctggg ccgtttcggc aaggtagtcc
     1681 cggtctcaat ccgggaatgc gccgcggccg ccggccgggt ctggaccccg catgacaggg
     1741 aagtgatccg caacgccccg gcggtcgatc cgtccctgcc cctgaaccgg gagcaggaac
     1801 tgcaggtgct cgactactac gggatcccgg aaacggtcgg ccgcggtgcc gagctcaagg
     1861 accgccccgc cggctcgaac acggccaacc cgcttacggc ctgacccgcg cccggctagc
     1921 agccgagcgc gcgggcgatc accatctgct ggacctcgtc ggttccctcg ccgatcgtga
     1981 ggatcttcgc gtcccggtag aaccggcaga ccgggtattc ctcgatgaat ccgtaaccgc
     2041 cgtggatctg gaccgcctcc tcggtggccc ggaccgccag ccggccggtg atcagcttgg
     2101 cctgggcggc ggtgagagtg aagttctcgc cccggtcctt ctcgatagcg gcccggtagg
     2161 tgagcagtcg ggcagcctcg agctgggccg agatgtcggc gatcttggcc tggatagcct
     2221 ggaacttgga gatcgactgg ccgaaggcct ggcgttcctt cgcgtacttg atcgcctcgt
     2281 cgagcgcacc ctgggcgacg ccgaccgaca tcgcggcgac accgatccgg ccgccgtcga
     2341 ggatctgcag gaactgcctg aacccctggc cccgggggcc gagcaggttt gactccggca
     2401 cccgggcgtc ctcgaaggtc agcgggtggg tgtcggaggc gttccagccc atcttgcggt
     2461 agggcgggtc aaccgagtag ccgggggttc cgttcgggac gatgatgttc gatatctccg
     2521 cccggccgtt ctcgtccttg ccggtgaccg cggtgatcgt gacgcaggcc gagatgtcgg
     2581 tgcctgaatt ggtgatgaac tgtttggcgc cgtttaccac ccattcgtcg ccgtcgagtt
     2641 cggcccgggt cttggtgttg cccgcgtccg atcccgcctc gggctcggtg agcccgaagg
     2701 cggccagcct tttgccggaa gtcagttcgg gcagccactc ggccttctgc tcttccgtgc
     2761 cccagttgta gatcggcgtg gtgccgagcg atgtgtgggc cgccatggtg atcgccaccg
     2821 aggaatcgat ccgggccagt tcctccaccg caagagcgta ggagaggatg tcgccaccgc
     2881 tgccgccgta ctcctcgggg aagggaatgc ccatcaggcc gagattgccg agcttctcga
     2941 cgatctcgta ggggaaggcc ttggtccggt ccagctcttc ggcgacgggc gcgacctcgc
     3001 tcacggcgaa atcccggact gtctccctga gcaggcgttg ctcgtcagtc agatcaaagt
     3061 tcatggctgg actttatgcg tcgggcgggc ccggatgccg cctggccttg ccatctctga
     3121 cgctgatgtt gagagttggt gaattcctcc cctgtgtgag atatctcata gtaggtcgca
     3181 tgatgcgact tactgcgata gtctcacagc cacgaagtgg ctttcccagc agagagccaa
     3241 acccgacccc gaaaaggggg caccatgaag cgcaatccga gaaaccgaac gagattccag
     3301 atgaggccgc tgaaaatcga cctcactgag gccgagattc tcgccgacct ccagtacggg
     3361 gtcagcggga aggtccgtca gggacgcgcc aaccgtcgcg ggaacgcagc caggtgattt
     3421 ccgggcgcga gatggcgagg gagcgggcgg ctgagctcgc ggcggaaatg ccggagatca
     3481 gtcgctcgct caccctcgag tggcaggacc cgatggcgat ggcctcgaag gggctcgagc
     3541 tggccggtct cgactacatg cgggcggtca tgaaccagga ggttccgccc ccgccgattg
     3601 cggtgctgat gaacatggct ccgaccgagg tcgaggaagg ccgtgccgtc ttctcggcgc
     3661 tcccggggga ggagcactac aacccgatcg gggtggtcca cggcggtttc atggcaaccc
     3721 tgctcgactc ggttgcaggc tgcgcggttc acaccaccct cgccctcggc gaggcctaca
     3781 cgacgctgac ccttgaagtg aagaacctga ggccgctgac cctcgacacg ggaattgtgc
     3841 gagccgaggg cctggtcacc taccggggcc ggcggcaggc gaccgccgac gcaaagctct
     3901 acgccgagga gaccggcaag ctgctcgcca gcgccagttc cacctgcatg atcatgagct
     3961 aaggtcgcgg gccagtgcga ccagcaggtg gacgccgaag ccggtcgggc ccttgcccca
     4021 gtactcgcga cccgtatccc aggcggtgcc ggcgatgtcg agatgcgccc agggcttgcc
     4081 ctctgtgaat tcctcgagga acgagccggc gtaaatcgtg ccggccttgc gctgggccga
     4141 ggcattgacc aagtcggtga tttctccctt ggtcagctcc ttgtactcgg gatggagcgg
     4201 caggcgccag accagctcgc ccgagcgctc ggcggcttcg gtgatctcgc ccgcgagccc
     4261 gtcatcgttc gagatcagcg ccgcgtaggt cgaacccagt ccgatcagta ccgccccggt
     4321 cagggtcgcg agatcgacca tccggtcggc gccttcccgg gcgcaatgca cgagagcgtc
     4381 ggccaggatc agccggccct cggcgtcggt gttggtgacc tcgaccgtct tgccgttgag
     4441 ctgcgtgatg atgtccccgg gcttcagggc ggtccctgac ggcatgttct cggtggaggg
     4501 gaggaccgcg atgatgtcga gcggcaggtc cagttcggcg atcgcatc
//
LOCUS       DAOTIP010000066         4542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22637_length_4542_cov_2.983731, whole genome shotgun sequence.
ACCESSION   DAOTIP010000066 DAOTIP010000000
VERSION     DAOTIP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22637_length_4542_cov_2.983731"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="P5138_02020"
     CDS             complement(<1..954)
                     /locus_tag="P5138_02020"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59383.1"
                     /translation="MTAALRRSFDSLSVPNFRRYFKGQVVSLSGNWMQTVAEVWLILS
                     LTGSGFAVGIATALQFLPIMLFGAWGGSLADRFAKRDLLMVTQAMMMVPPLILLGVTV
                     AGVVTPAMVFALILIRGSLLSVDNPARQAFVMEMVGPDRIVNAVSLNSVIVHSARVAG
                     PAVAGILIATVGVAPCFGLNAATFLVMIWALRGMDRRRLAEPPKTNSREPGRVRAAVR
                     HVMQTPELAMPLMLMAVVGTLGLNFQVILPLLARLTFEGGASSYAVLVSSMGAGSVIG
                     ALVTGARGKTGMGVIAASALAFGGLAMAAALMPSLASEIPVL"
     gene            complement(951..1532)
                     /locus_tag="P5138_02025"
     CDS             complement(951..1532)
                     /locus_tag="P5138_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRV59384.1"
                     /translation="MSEPETNSQADSQTKRQTTQDVEVQKVDAHRDITGVQDRRAEAD
                     ALRLAITRTARRLRQEAGGGLSPTLIATLASIERGKVLTPGELARIEGVQRPTMTRAI
                     NRLADAGLIDRREDPEDRRSALLSVSEEGHRYLAAHRSRKSAWLANLIEEMPADDADT
                     LARAAEILSHALETHRTGELRLPGEDGNSGEGS"
     gene            1732..2484
                     /locus_tag="P5138_02030"
     CDS             1732..2484
                     /locus_tag="P5138_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRV59385.1"
                     /translation="MADKDDQKGTDDPAAGGDHHLRETFRAELTGGTQPDNGVEGPRA
                     IKAERIMNGPLMVAAALTLPSVALTEAQVGGTLQVVAQVLNWGTWLAFAIEFVVMLAL
                     VPNRRKYLRHHPVEVIVVFLTPPVLPPGLQSLRAIRLLRLLRLLKLAQLSNRVFSNEG
                     LQYAALLTVLATVAGGSVFRAFEAKNQHLTEWDGVFWAFTTMTTVGSEFTATTVGGQI
                     TEVVILLVGISFVAMLTGSIAQRFLKPSSDSK"
     gene            complement(2617..3846)
                     /locus_tag="P5138_02035"
     CDS             complement(2617..3846)
                     /locus_tag="P5138_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59386.1"
                     /translation="MQTGLPQTPSGRARGFSLAAALIVAALAIGLACWARPADATLTA
                     DQTQAKIDAINGSVSDLKAQVAEDNRKIDSLIGELSGLRARANELEAKLAAKQAELDA
                     VEAALEREREHLAEVRARLRRALKILEDQLVSLYVNGSPNVEDMVLSSSDFGELVSSA
                     GYANAIQDRNESVITRVEELRDQIKAMVEKMKVQQKKLQAARDEIAVETKQAEDARDA
                     VESRKAEFEASSAARQARIDALEAKAGKLEDSLPDLTMDPASSSAPRAVPPVAGSVAV
                     INSDGTASPPADAPQAVKDVIAAGNQIIDLPYVWGGGHGSFESSGYDCSGTVSYALHG
                     GGLISSPLDSTGLTTWGEPGEGNWITVYGNSGHAFMHVAGISLDTSGTGGSGPSWSSD
                     LGWEDTSAFVARHPSGL"
     gene            complement(3956..>4542)
                     /locus_tag="P5138_02040"
     CDS             complement(3956..>4542)
                     /locus_tag="P5138_02040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HRV59387.1"
                     /translation="LIGFFTGQWPSGHPYRSITAVPGEGVMPEIWLLGSSGYTAQVAG
                     LLGMPYSFAHHFSAGNTEAAVQLYRSRFEPSAYLDEPRVMIGVGVICAETDEEARWLA
                     GPSRLAFSRLRSGRPGRFPTPEEAAAHDFTPDEEAVISSIAKGHIFGSSETVKSGLDD
                     LIEKTGADELIISTMVYEQKDRLKSYELVADIEL"
BASE COUNT          760 a         1425 c         1542 g          815 t
ORIGIN      
        1 gagaaccgga atctcggatg cgaggctggg catcagcgcc gcggccatcg ccaggccgcc
       61 aaaggccagg gcggaggcgg cgatcacacc cattccggtc ttgccgcggg cgccggtcac
      121 gagggccccg atcaccgaac cggcacccat cgaagacacc aggaccgcat acgaactggc
      181 cccgccctcg aaggtgaggc gggcgagcag aggcaggatc acctggaagt tgagaccgag
      241 ggttcccacc acggccatca gcatcagcgg catcgccagt tccggggtct gcatcacatg
      301 cctgaccgcg gcccgtaccc ggccgggctc gcgactattg gtcttcggcg gttcggccag
      361 gcgcctgcgg tccatgcccc tgagggccca gatcatgacc aggaaggttg ccgcgttgag
      421 cccgaaacag ggcgcgactc cgacggtggc gatcaggatg cccgcgaccg ccggacccgc
      481 gacgcgggcg gagtggacga tcacgctgtt gaggctgacc gcgttgacga tccggtcggg
      541 tccgaccatc tccatgacga aggcctggcg ggccgggttg tcgaccgaaa gcaggctgcc
      601 ccggatgagg atcagggcga agaccatggc tggcgtgacg actccggcga cggtgacacc
      661 gagcaggatc agtggcggga ccatcatcat ggcctgggtg accatgagca gatcccgctt
      721 ggcaaagcgg tctgccagcg atcctcccca tgcgccgaac aacatgatcg gcaggaactg
      781 gagggccgtg gcgatcccga ccgcaaagcc ggagccggtc aggctgagga tcagccagac
      841 ctcggcaacg gtctgcatcc agttccccga gagggaaacg acctgtccct tgaaatagcg
      901 gcggaagttt gggaccgaga gagagtcgaa ggaccgccgc agcgcggccg tcaactcccc
      961 tccccggaat tgccgtcttc accggggagg cggagctcgc cggttcgatg ggtttcgagg
     1021 gcgtgggaga ggatttctgc cgcccgggcg agggtgtccg cgtcatcggc cggcatttcc
     1081 tcgatcaggt tggccagcca ggcactcttg cgcgaacgat gggcggccag gtaccggtgt
     1141 ccttcttcgc tgaccgaaag cagggctgaa cgacggtcct ccgggtcctc gcggcgatcg
     1201 atcaggcccg catcggcgag ccggttgatc gcacgggtca tggtcggacg ctgaacccct
     1261 tcgatccggg caagttcacc cggggtcagc accttgcccc gctcgatgga ggccagggtg
     1321 gctatcagcg tgggggaaag gccgccaccg gcttcctggc gaaggcgcct ggcggtgcgg
     1381 gtgatcgcca gtcgcagggc atcggcctcc gcgcggcggt cctggacccc ggttatgtcc
     1441 ctgtgggcat caaccttctg gacttctacg tcttgcgtcg tctgacgctt tgtctgacta
     1501 tctgcctgac tatttgtttc tggctctgac attgactttg cctacttgca ctctgtttcg
     1561 ggatctcgga ccgggcgcct ggcccgggag ttcaaccgaa aatcattacc tgtgctaatt
     1621 agtttagcga agtagtttgt cagagtcaat ggcacttcga tgtacccgga attggccggg
     1681 atttccgatg cggctacagg cgccttggcg gtcacgtcga atacggctcc gatggccgac
     1741 aaggacgacc agaaaggcac cgacgacccg gcggcagggg gagatcacca cctgcgtgag
     1801 accttcaggg ccgagctgac cggcggcacc cagcctgaca acggagtcga gggcccgcgg
     1861 gcgatcaagg cggagcggat catgaacggt ccgctgatgg tggcggccgc gctgaccctg
     1921 cccagcgtcg cgttgaccga ggcccaggtc gggggaacgc ttcaagttgt tgcccaggtg
     1981 ctcaattggg gtacctggct tgcgtttgcg atcgagttcg tggtgatgct ggccctggtg
     2041 cccaaccgtc gcaagtacct gaggcaccac ccggtcgaag tcatcgtcgt cttcctcacc
     2101 ccgcccgttc tgccccccgg cctccagagc ctgagggcga tccgcctgct ccgcctcctg
     2161 cgccttctca agctggccca gctttcaaac cgggtcttct cgaacgaagg tcttcagtat
     2221 gcagctctgc tgacggtact cgccacagtc gccggtgggt ccgtctttcg ggcatttgaa
     2281 gccaagaacc agcacctgac cgaatgggac ggtgtcttct gggccttcac caccatgacc
     2341 acggtcggtt ctgagttcac ggccaccacg gtcggcggcc agatcaccga ggtagtgatc
     2401 ctgctggtcg ggatctcctt cgtggccatg ctgaccggct cgatcgccca gcgcttcctc
     2461 aagccctcct cggacagcaa gtagcgcctt ccggcctctg cccggaagtc atccagcgga
     2521 ggtgaaacac atgcgggtac gtgagctgcc gtttccggcc gtcgacgaag gtcagcgtcg
     2581 ttctggtggt tccgggacga agcccggggc agcaggttag aggccggagg ggtggcgggc
     2641 cacgaaggcg gaggtgtctt cccagccgag gtcgcttgac cagctggggc cggagccgcc
     2701 ggtgcccgac gtgtcgaggc tgatgccggc gacatgcatg aaggcatgtc cggagttgcc
     2761 gtagacggtg atccagttgc cttctcccgg ctcgccccag gtggtcaggc cggtcgagtc
     2821 gagcgggctg gagatcaggc cgcctccgtg gagggcgtag ctgaccgtgc cggagcagtc
     2881 ataaccggaa gattcgaagg atccgtggcc tccaccccat acatagggca ggtcgatgat
     2941 ctggttgccg gccgcgatca cgtccttgac tgcctgcggc gcgtcggcgg gcggtgaagc
     3001 ggttccgtcg gagttgatca ctgcgaccga gccggcgacc gggggcactg cccggggcgc
     3061 ggagctggag gccgggtcca tggtcaggtc gggcagcgag tcctcgagct tgccggcctt
     3121 cgcttcgagc gcgtcgatcc tggcctggcg ggctgcgctc gatgcctcga attcggcctt
     3181 cctggattcg accgcatcgc gggcgtcctc ggcctgtttg gtctcgacgg cgatctcgtc
     3241 cctagccgcc tgaagcttct tctgctggac cttcatcttc tcgaccatcg ccttgatctg
     3301 gtcacgaagc tcttcgaccc gggtgatcac cgactcgttt cggtcctgga tcgcgttggc
     3361 atagccggcg ctggaaacga gctcaccgaa atcggaggac gagagcacca tgtcctcgac
     3421 attcggggat ccgttcacgt aaagcgagac aagctggtct tcgaggatct tcagcgcccg
     3481 acgcaggcgg gccctgactt cggccaagtg ctcacgttcg cgctcgaggg cggcttccac
     3541 cgcgtcgagc tcagcctgct tggcggcgag tttggcttca agttcgttgg cccgggccct
     3601 tagcccggat agttcgccga tcagcgagtc gatcttccgg ttgtcctcgg cgacctgcgc
     3661 cttgagatcg gagacggatc cgttgatcgc gtcgatcttc gcctgggtct ggtcggccgt
     3721 gagcgttgcg tcagccggac gcgcccaaca ggcgaggccg atggcaaggg cggcaacgat
     3781 cagggccgca gcaaggctga agccgcgggc gcggccgctg ggtgtttgcg ggagtccggt
     3841 ctgcataaag gcccgccgga gaggacgggc ggaaggggaa ggtagcgtta tggcggtccc
     3901 ggaccgagct cagggcgatc ggtgggaaat cccgtgcaag tggaggaaaa cggctctaca
     3961 gctcgatgtc tgcgacgagc tcgtaggact tcaggcggtc cttctgctcg tagaccatgg
     4021 tcgagatgat cagttcgtcg gccccggtct tctcgatcag gtcatcgagt cctgacttca
     4081 ccgtttcgga gctgccgaat atatgcccct tggcgatcga agagatcacc gcctcctcat
     4141 ccggggtgaa atcgtgcgcg gccgcttcct ccggggtcgg gaacctgccg ggccggccac
     4201 tccggaggcg ggagaaggcg agtctcgagg ggccggcaag ccagcgggct tcttcgtcgg
     4261 tttcggcgca gatcacgccc acgccgatca tcacccgggg ctcgtccagg taggccgagg
     4321 gctcgaaacg ggagcggtag agctggacgg cggcctcggt gttcccggcg ctgaagtggt
     4381 gggcgaatga gtacggcata ccgagcaggc cggcaacctg ggctgtgtat ccgctggaac
     4441 cgagcagcca gatctccggc atgaccccct ccccgggcac cgcggtgatc gaccggtacg
     4501 gatggccgga tggccactga ccggtaaaga agccgatcag tt
//
LOCUS       DAOTIP010000067         4518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22797_length_4518_cov_2.785122, whole genome shotgun sequence.
ACCESSION   DAOTIP010000067 DAOTIP010000000
VERSION     DAOTIP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22797_length_4518_cov_2.785122"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1225
                     /locus_tag="P5138_02045"
     CDS             209..1225
                     /locus_tag="P5138_02045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRV59388.1"
                     /translation="MMLNFDVDRYLDIQSGAVNLAEGLDRTIGDLLDGGKKTLIFAGA
                     GGAGMLMQPAAQLLMRRSRFPVFWDRPAEMMAIRPVYFDENAVVIIPSLSGTTMESIE
                     LVEASREVGAVTIALTGHEDSPIAGSVDHNFTNFAEDDTSSESFYLQSLLVALSVLRR
                     EPDRVDYDSVVSELKKLPELLVDAKAAFEDRAEEWAEAFRDEDYHIISAAGPLWAEAW
                     YYGTCILEEMQWIRTRPIHAADFFHGTLELLEPEVSLILLKGSGPSRELENRVEEFAG
                     TISNKVRVLDTDDVDMPGISDPVREMVSPAIASALLERVSAHLEVKRDHPLTTRRYYR
                     KVAY"
     gene            1402..1776
                     /locus_tag="P5138_02050"
     CDS             1402..1776
                     /locus_tag="P5138_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59389.1"
                     /translation="MPKKTETPLVMGTVSQRREPVWDPLEYMIGSRAREFMWMFEVEM
                     EDGTAVQAYKHVFTRKYFHLGSDGRSFEFRSFGGEGPSGYEQVPFLRTAGMVLWLYDQ
                     ERETLWKPGEREYCNCPLNCWD"
     gene            complement(1773..2198)
                     /locus_tag="P5138_02055"
     CDS             complement(1773..2198)
                     /locus_tag="P5138_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRV59390.1"
                     /translation="MEELYREQILEHYKRPHNFGAVENPDLEFEDTNPFCGDEQHVTI
                     RLDEDDRVAEVKFEGQGCAISTAATSLLTDELIGMSRDELIKLPKEYVLDLLGIDISA
                     TRMKCALLGLKVVKSAGLGQTTDWEDAGEQGDPSLASEI"
     gene            complement(2252..2545)
                     /locus_tag="P5138_02060"
     CDS             complement(2252..2545)
                     /locus_tag="P5138_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59391.1"
                     /translation="MDPEDDLVIFPREDVEACLRNEKMQQEANSDGSPSGQSQAMETS
                     RLGAIEAGAPERIRWIYFESPEWTWQNLCGRSGWLLIDPETIDQYDFVMTMMN"
     gene            complement(2589..3638)
                     /locus_tag="P5138_02065"
     CDS             complement(2589..3638)
                     /locus_tag="P5138_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59392.1"
                     /translation="MEVKLGVPEGEADEFDTLPPADLPAHEKKLIDARNHLVQTSQAA
                     RIWAEENPAHPVSYLVTRSDVSEQTKIQALRRIESVSLPFQVNELLDAARHFNECQAV
                     SQAAFDAEQPVAEDDLPDHGDRDIFEGLRAAYLNLLRGYRNWGGFGYHGWTTKRDPDS
                     YPGPAIWTRQDCVFRFGRALEREFPDRVHLGLKISKSTTESFDPAVDRRQSVDITVTV
                     PGLFSRDLSARRFRNLRHDLFVEVEWLKKGRWLNTDQLVKRCGRIQQNLINLQRSIDS
                     GRCEFAAMLIVDDEGFLDFHGDLLRWPPDVIPLVVSPGEVVELGLDDGSVEPVLDEIE
                     SLHDSACPCSDGPLA"
     gene            complement(3738..4325)
                     /locus_tag="P5138_02070"
     CDS             complement(3738..4325)
                     /locus_tag="P5138_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59393.1"
                     /translation="MSQRRITPSLVISVIALIVALGGASYAALRIPRNSVGAGQLKRN
                     SVSASKLRKGSVNSSKVKDRSIRSRDLAPGVIPGATWRGGRDSSTLLDLTGTMQVVAS
                     TPVLPAGSYVVWSRANVLGGVGSSTIICSVASDAAQNITIPAGAVFPLSMAATKVLEE
                     PGPIELNCNKSSGSPQVAQAQVIATRVPKLTAPPE"
     gene            complement(4371..>4518)
                     /locus_tag="P5138_02075"
     CDS             complement(4371..>4518)
                     /locus_tag="P5138_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV59394.1"
                     /translation="AGHHCAQILMRRLGVAATTRASFAVYNTTEEIDTLVEALHDARR
                     IFGL"
BASE COUNT          844 a         1355 c         1463 g          856 t
ORIGIN      
        1 ccgccaataa ccgtggtccg gcaaccggtc tatgcgctgg gccaggcaac cggacggctg
       61 ctgaacgacc ggatcaacgg ctggacggga gatccgaaac aactccgcct gaaaaccgaa
      121 ctggtcgtcc gggaatcctg ccgcaagtgg caggagcctt cctgacccag gtcaataacc
      181 ccgatcaaca tgcgcatgaa caaggtgaat gatgcttaat ttcgatgtag acagatacct
      241 ggatatccag tccggcgcgg tcaacctcgc cgaaggactg gaccggacaa tcggcgacct
      301 gctggacggg ggcaagaaaa ccttgatctt cgccggagcg ggaggggccg ggatgctgat
      361 gcagccggcg gcgcaactgc ttatgcggcg atcccgcttc ccggtcttct gggaccggcc
      421 agccgaaatg atggcgatca ggcccgttta cttcgacgag aacgcggtcg tgatcatccc
      481 ctcactgtca gggaccacga tggagagcat cgaactggtc gaagccagtc gggaggtcgg
      541 cgccgtcact atcgccctga ccggtcacga ggattcaccc atcgccgggt cggtcgatca
      601 caacttcacc aacttcgccg aagatgacac ctcgtcggag tcgttctatc tccagtccct
      661 gcttgtcgcg ctttcggtgc tccggcggga gccagacagg gttgactacg actcggtcgt
      721 cagcgaactg aagaagcttc ccgaactgct cgttgacgca aaggccgctt tcgaagatcg
      781 cgccgaggag tgggccgaag cctttcgcga cgaggattac cacatcattt ccgccgccgg
      841 gccgctctgg gcggaagcct ggtactacgg gacctgcatt ctcgaggaga tgcagtggat
      901 ccggacccgg ccgattcacg cagcggattt cttccacggc acgctcgagt tgctcgagcc
      961 cgaagtcagc ttgatcctgc tgaagggaag cggaccctct cgggaactcg agaacagggt
     1021 cgaggaattt gccgggacga tctcaaacaa ggtacgagtc ctggacacgg acgatgtcga
     1081 tatgccgggg atatcggacc ccgtacggga aatggtctca cccgcaattg cctccgcgtt
     1141 gctggaacgg gtcagcgccc acctcgaagt caagcgggat catccactga ctacccgccg
     1201 ctactacagg aaagtcgcct actgaagccc tttgagaacc aggacgtgga ttgaatcacc
     1261 cagtgagcga atcgaaggcg agaagctgga tcgagtttcc ccgcgcattt cgcccgtctg
     1321 ctgcgctttc gtctccattt cgtcacccaa ttgttgctat gaagtcttgt ttcgtcacac
     1381 cttttcgcgc atggtcgggg catgccgaag aagaccgaaa caccacttgt tatgggtact
     1441 gtctcccaac ggcgagagcc cgtctgggat cccctcgaat acatgattgg ctcgcgagcc
     1501 cgcgagttca tgtggatgtt cgaggtcgag atggaggatg ggacggcggt ccaggcctac
     1561 aagcacgtct tcacgcgaaa gtacttccac ctggggagtg acggtcgatc gttcgagttc
     1621 cgaagctttg ggggcgaagg gccctcgggg tatgagcagg ttcccttcct gaggacagcc
     1681 ggaatggtgc tttggctgta tgaccaggag cgggagactc tttggaaacc cggcgaacgt
     1741 gagtactgca actgcccgct gaactgctgg gactaaatct cggaggccag ggaggggtcg
     1801 ccttgttctc cggcgtcctc ccagtcggtt gtctggccga ggccggctga cttgaccacc
     1861 ttgaggccta ggagggcgca cttcatgcgg gtggcggaga tgtcgattcc gaggaggtca
     1921 aggacgtact ccttggggag cttgatcaat tcgtcgcggg acatgccgat gagctcgtcg
     1981 gtgaggagag aggtggctgc ggtggagatc gcacagccct ggccttcgaa cttgacctcg
     2041 gcgacccggt cgtcttcgtc gaggcgaatc gtgacgtgct gctcgtcgcc gcagaagggg
     2101 ttcgtgtcct cgaactcgag gtccgggttc tcgaccgccc cgaagttgtg ggggcgcttg
     2161 taatgctcga ggatctgttc gcggtagagc tcttccatcg gtcagacgag gttagctgac
     2221 cgccgctccc aggccacgaa cccttgccgg gtcagttcat cattgtcatc acgaagtcgt
     2281 actggtcaat cgtttccgga tcgatgagca accagccgga gcgcccgcaa aggttctgcc
     2341 aggtccattc gggtgactcg aagtagatcc accggattcg ctcgggtgct ccggcctcaa
     2401 tcgcgccgag ccgactcgtt tccatcgcct gactctgccc ggacggagaa ccgtccgaat
     2461 tggcttcctg ctgcatcttc tcgttccgca aacacgcttc aacgtcttcc cggggaaaga
     2521 tcaccaggtc atcttccggg tccatgagtt gagagtcgcg agcccggccc ggacctgaca
     2581 gggtccggtc aggccagggg gccgtcggag caggggcagg cggagtcgtg gagggattcg
     2641 atttcgtcga ggaccggttc gaccgagccg tcgtcgaggc cgagctcaac cacctctccc
     2701 gggctgacca ccaggggaat cacgtccggc ggccacctga gaaggtcgcc gtggaagtcg
     2761 aggaagcctt cgtcgtcgac gatcagcatc gcggcgaact cgcagcgccc gctgtcgatc
     2821 gagcgctgca ggttgatcag gttctgctgg atccggccgc agcgcttgac cagctggtcg
     2881 gtgttcagcc agcggccctt cttcagccac tcaacctcga cgaagagatc gtggcgcagg
     2941 ttccggaacc ggcgtgcgct caggtccctg gagaacaatc cgggcacggt gaccgtgatg
     3001 tccacggact gccggcggtc aaccgccggg tcaaagctct ccgtcgtcga cttgctgatc
     3061 ttcaggccga ggtgaacccg gtccggaaac tcccgctcca gcgcccgtcc gaaccggaac
     3121 acgcagtcct ggcgggtcca gatcgccggg ccgggatagc tgtccgggtc ccgcttcgtg
     3181 gtccagccgt ggtagccgaa gccaccccag ttgcgatagc cgcgaagcag gttgagatag
     3241 gcggcacgca ggccttcgaa gatatcccgg tcaccatggt ctgggaggtc gtcctcggcg
     3301 actggctgct cggcatcaaa ggcggcctgg gaaacggcct ggcattcgtt gaagtggcgg
     3361 gccgcgtcaa ggagttcgtt cacctggaag ggcagtgaaa ccgactcgat cctccgcaag
     3421 gcctggatct tggtttgttc actgacgtcg ctgcgggtga cgagatagga aacagggtgg
     3481 gccgggttct cctcggccca gatccgggcc gcctggctgg tctggaccag gtggttccgg
     3541 gcgtcaatca gtttcttctc gtgggccggc aaatcggcag gtggaagcgt gtcgaactcg
     3601 tcggcttctc cctccggcac ccccaacttg acttccatgc gcgaaatcgt agatgtggtc
     3661 tgctgttgcg gggggtgaaa cgggtcagac tgaaggcagt cctgcacggg gacggtgacg
     3721 ttccccgggg agcggaatca ttccgggggt gcggtgagtt tcgggacccg cgtcgcgatg
     3781 acctgggcct gcgccacctg cggggaaccg ctggacttgt tgcagttgag ctcgatcggg
     3841 ccgggctctt cgaggacctt ggtcgctgcc atcgagagcg ggaagaccgc acccgccggg
     3901 atggtgatgt tttgcgccgc gtcggaagcg accgagcaga tgatcgtcga actgccgacg
     3961 ccgccaagga cattcgcccg gctccagacg acatatgaac cggccggaag cacgggcgtg
     4021 ctcgcaacca cctgcatcgt cccggtcaga tcaagcagcg tcgaggagtc ccggcctcct
     4081 cgccaggtgg cgccgggaat cactcccggc gccagatccc gggaccggat cgaccggtct
     4141 ttgaccttcg acgagttgac cgaccccttc ctcaacttgg acgccgaaac ggagttcctt
     4201 ttcagttgac cggcgccgac cgagtttcgc ggaatcctga gggccgcgta gctggcacca
     4261 cccagggcga caatcagcgc gatgacggaa atgacgagcg agggcgtgat tcgacgttga
     4321 gacatgccct tggcttacac cgccgggacg gtcctgtcaa cgccaaccgt tcagaggccg
     4381 aagatgcggc gggcgtcatg gagggcttcg accagggtgt cgatttcctc cgtggtgttg
     4441 taaacggcga agctggcgcg ggtcgtggcg gcgacgccga ggcggcgcat caggatctgg
     4501 gcgcagtgat gaccggcc
//
LOCUS       DAOTIP010000068         4507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22855_length_4507_cov_2.825022, whole genome shotgun sequence.
ACCESSION   DAOTIP010000068 DAOTIP010000000
VERSION     DAOTIP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22855_length_4507_cov_2.825022"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="P5138_02080"
     CDS             complement(<1..243)
                     /locus_tag="P5138_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59395.1"
                     /translation="MWGRLKKLWNRSSFSNLFALVAVFLVLGGASYAAIKIPKNSVGT
                     RQLKKNAVNSSKVKNRSLLAVDFRKGQLPRGATGAPG"
     gene            complement(335..1225)
                     /locus_tag="P5138_02085"
     CDS             complement(335..1225)
                     /locus_tag="P5138_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRV59396.1"
                     /translation="MSTRGDRRRSAARKRRAAGRRRLFVAAAILVALGTIVWAGLNVS
                     RLWKTDTHGADVTETSIKSKAVPGDHPVSVVVPEGAGDGESGMLVFLHGRSGDNGSYT
                     NDEEFFEALAAQGDEAPIVVFPDGGESSYWHDRDTGDWGTYVTDEVIPQMAEKFGADP
                     DRVAIGGISMGGFGALDLAEKNPDRFCAVGGHSPALFLSGGESAPGAFDDAEDFDEND
                     VLATAASDPGALSSQPVFLDAGKSDPFVPGVTQLAGSLEAAGDDATVHLNWPGGHDED
                     YWNAHWDDYLRFYADALASC"
     gene            1314..1385
                     /locus_tag="P5138_02090"
     tRNA            1314..1385
                     /locus_tag="P5138_02090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Val,seq:cac)
     gene            complement(1399..2112)
                     /locus_tag="P5138_02095"
     CDS             complement(1399..2112)
                     /locus_tag="P5138_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV59397.1"
                     /translation="MNLIANSNRSRGERRRIKTRTAILDAAENAFTELGYRRTKMEDV
                     SEAADVAVGSIYSHFGSKDGLYAALVERAVELFASYLQEAYDPDWSPLEQVIACGDAY
                     LRFHIEHPGAFRFLAFDGVETDTPPIDPGQSERMNARVDAIVGQFEATVQAAIDAGEI
                     RSSVDPELISRFLWGAWNGTVALGLRRDGFYLSEDRIEEAIQQARRIVLEGLSSVQFR
                     GDDGNSRAEMRTVESPRSD"
     gene            2192..3649
                     /locus_tag="P5138_02100"
     CDS             2192..3649
                     /locus_tag="P5138_02100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV59398.1"
                     /translation="MEDADVVVVGSRLAGCAVAAPVARSGRKVIVIDRMTFPSDQLST
                     HLLTTTGVSEIAKMGALPRVLATNPTRVSKATFICEGIPCSERLRPADDGTDFGICVP
                     RDLQDICLVEAAREQGADIRESCTFEDLLWENGRVAGIRYKDESGKRREVRATLTVGA
                     DGRRSNVAARAGAWTPYRMSKNGRGLVFRYMDDPKPGTKDADTFIVWREDRSMAFAFP
                     SAPAGKLLVLFMGHRDEVSAARKDPEGYWQSKLDQHSGMKERVSGVDTESLTKVRSTG
                     DCPAFFRASSGPGWALAGDAGHFKDPVTGQGMRDAMWMGRTLAEGVIADLDDPAGVDK
                     ATRQWEASRDRHCLPAYHTANLDTRIETQSAVMREIFRDAGRNEESDLSDIFTRHRTQ
                     QEIVGPRRMVRALTLALARGEQPRGETISRSIRDLRTEVAIRREIRDDVFRQTRTIKG
                     SDHPGVEWPAPPKAPGAAPGAKEGRPEADSMEVSS"
     gene            3691..>4507
                     /locus_tag="P5138_02105"
     CDS             3691..>4507
                     /locus_tag="P5138_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRV59399.1"
                     /translation="MEANLLRVEELIRDAFREHSPEVILVPEGCSSPNVFAKVLLGSA
                     RPVDGQPFQMLTRLARELGCVIGGGFVAVRGNDTFGTYVLAEPDGSIHLHDKDIPTAW
                     ENNYYRRGDDAGVVRSNSLDCRVGLMSGWEWARSGTSRRVRQAGAQLVLGGMCWPSMP
                     TNWPGPLKLLTSREHRIWKEQARALPGQVARITGAPVAIASHVGPITGETPLIPGLRW
                     PTTMIGESQICDRDGMVLARLSEEDGEGHVSAEVQLGEPAPAESIPDSYWIPRM"
BASE COUNT          807 a         1518 c         1433 g          749 t
ORIGIN      
        1 cccgggggct ccggtcgcgc ctctcggaag ctgccccttc ctgaaatcga ccgcgagaag
       61 cgaacggttc ttcaccttgg acgaattgac ggcgttcttc ttcagctgtc tggtcccgac
      121 actgttcttc ggaatcttga tcgccgcgta ggaagcacca ccgagaacca ggaaaacggc
      181 caccagggcg aacaggttcg agaacgacga gcgattccac aacttcttca acctccccca
      241 catgacccga aactatccgg cctccgcgta aattgccagc cttgggcaga tgccagacca
      301 gaacgaaatc cctggtcacg ggtaaggatc ttcttcagca gctggcgagc gcgtccgcgt
      361 agaagcggag gtaatcgtcc cagtgggcgt tccagtaatc ctcgtcgtgg ccgccgggcc
      421 agttgaggtg gacggtggca tcgtcgcccg cagcttcgag cgaaccggca agttgagtca
      481 ccccgggcac gaaagggtcg ctcttgccgg catcgaggaa gaccggctgg gacgagagcg
      541 cgccggggtc cgacgcagcg gtggcgagca catcgttttc gtcgaagtcc tcggcgtcgt
      601 cgaaggcccc cggggcggat tcgccaccgc tcaggaacag ggccggagag tggccgccga
      661 cggcacagaa ccggtccggg ttcttctccg ccaggtcaag cgcgccgaat ccgcccatag
      721 agatccctcc gatcgccacc cggtccgggt cggccccgaa cttctccgcc atctgcggga
      781 tcacctcgtc ggtgacgtag gtgccccagt ctccggtgtc ccggtcatgc cagtagctgg
      841 actcgccgcc gtcggggaac accacgatcg gcgcctcgtc gccctgggcc gccagcgcct
      901 cgaagaactc ctcgtcgttc gtgtagctgc cgttgtcgcc gctgcggcca tggagaaaga
      961 ccagcatgcc gctctcgccg tcaccggccc cttccggcac gaccaccgac accgggtgat
     1021 cacccgggac cgccttcgac ttgatcgagg tctcggtcac gtccgccccg tgcgtgtcgg
     1081 tcttccagag gcggctgacg ttaagtcctg cccagacgat tgtccccagc gctaccagga
     1141 ttgccgcggc gacgaagagc cttcgccgtc cggcggctct gcgcttccgt gctgcggacc
     1201 gtctcctgtc tccccgggtg gacacggaca aaaactagcc gcaccacccc gacagatcag
     1261 ctccggcccc acccggtcag agaattgcca ctccaactac aatgccgtct cccgggcgcg
     1321 tagctcagcg ggagagcgct cgcttcacac gcgagaggtc gctggttcga aaccagccgt
     1381 gcccatcacc gaagcccgtc aatccgatcg cggcgactcc acggtcctca tctcggctct
     1441 cgagttgccg tcatctccgc ggaactgaac cgatgacagg ccctccagga cgatccggcg
     1501 ggcctgctgg atggcctctt cgatccggtc ttcgctcagg tagaagccgt cccgcctcag
     1561 accgagggcg acggtgccgt tccaggcccc ccagagaaac cggctgatca gttcgggatc
     1621 gaccgagctg cggatttcgc ccgcgtcgat cgccgcctgg accgttgcct cgaactgccc
     1681 gacgatcgcg tccaccctgg cgttcatgcg ctcactctgt ccgggatcga tgggcggcgt
     1741 atccgtctcg actccatcga aggcgaggaa acgaaaggcg cccgggtgct cgatgtgaaa
     1801 ccggagatag gcgtcaccgc aggcgatgac ctgctccagc ggtgaccagt ccgggtcgta
     1861 agcctcctgc aggtagcttg cgaagagttc aacggcccgc tcgaccaggg ccgcgtacag
     1921 cccgtccttg ctgccgaagt gcgagtagat ggagccgacg gcaacgtcgg ctgcttcgga
     1981 gacgtcctcc attttcgtcc tgcgatagcc aagctcggtg aacgcgttct ccgccgcatc
     2041 gaggatcgcg gtgcgggtct tgatcctgcg cctttcacca cgggaacgat tgctatttgc
     2101 aatcaggttc atagttgaac tatcttacaa atcagtccgg attcgatgta atctccggac
     2161 ctgagggccg aacccgtccg aggaggggaa ggtggaagac gcagatgtcg tggtagtcgg
     2221 ttcccggctt gccggttgcg ccgttgcggc gcccgtcgca cgctcgggcc gcaaggtcat
     2281 cgtgatcgac cggatgacct tcccctccga tcagctttcg acccacctgc tgacgaccac
     2341 cggcgtatcc gagatcgcga agatgggtgc tttgccgagg gtacttgcga ccaatccgac
     2401 ccgggtcagc aaggcgacct tcatctgcga gggcatcccc tgctccgagc ggctcagacc
     2461 ggccgacgac gggaccgact tcggaatctg cgtgcctcgc gaccttcagg acatctgcct
     2521 ggtcgaggcg gcccgcgagc agggggccga catccgcgag agctgcacgt tcgaagacct
     2581 gctctgggag aacggtcgcg tcgcgggcat caggtacaag gacgaaagcg gcaagcggcg
     2641 cgaggtccgg gcgaccctca cggtcggcgc cgacggccgt cggtcgaacg tggccgcaag
     2701 ggccggggcc tggaccccct accggatgtc gaagaacggt cgcggactcg tattccgtta
     2761 catggacgac ccgaagccgg gaacgaaaga cgccgacacc ttcatcgtct ggcgcgagga
     2821 caggagcatg gcgttcgcct tcccctccgc cccggccgga aagctcctgg tcctgttcat
     2881 ggggcatcgc gacgaggtct ccgccgcccg caaggacccc gagggctatt ggcagtcgaa
     2941 actcgaccag cattcgggaa tgaaggagcg cgtttcgggc gtcgacaccg aaagcctcac
     3001 caaggttcgc tctacgggtg attgcccggc gttcttccgc gcttcttcgg ggcccggctg
     3061 ggcactggcc ggcgatgccg ggcacttcaa ggacccggtc accggtcagg ggatgcgaga
     3121 cgcgatgtgg atgggacgga cgctggccga aggcgtgatc gccgatctcg acgacccggc
     3181 cggagttgac aaggcgaccc ggcaatggga ggccagccgg gaccggcatt gcctgccggc
     3241 gtaccacacc gcgaatctgg acacgcggat cgaaacccag tcggcggtga tgcgcgagat
     3301 cttccgggac gccggtcgaa acgaagagtc ggacctgagc gacatcttca cccgccaccg
     3361 cacccagcag gaaatcgtcg gcccgcgacg gatggtccgg gcgctgaccc tggccctggc
     3421 ccgcggagag cagccaaggg gcgaaacgat ctcccggtcg atcagggacc tccgcaccga
     3481 ggtcgcaatc cggcgggaaa tccgtgacga cgttttcagg cagacccgga ccatcaaggg
     3541 ctccgaccat cccggggtcg aatggcctgc cccgcccaag gctccggggg ccgcgccagg
     3601 ggcgaaagaa ggacggcctg aagcggactc gatggaggtt tcatcctgac ccggatctcg
     3661 gtcatccagc ccgcccttgc cctgggcgaa gtggaggcca atctcctccg cgtcgaggaa
     3721 ctgatccgcg acgcctttcg agaacacagc cccgaggtga tcctcgttcc cgaaggctgt
     3781 tcgtcgccca acgttttcgc aaaggtgctg ctcggttctg cgcgaccggt cgatggccag
     3841 ccgttccaga tgctcacccg tctcgcccgg gaactcggat gcgtgatcgg cgggggcttc
     3901 gtggccgttc gcggcaacga caccttcggg acctatgtcc tcgccgagcc cgatggctcg
     3961 atccacctcc acgacaagga catcccgacc gcatgggaga acaactacta ccggcgcggt
     4021 gacgacgcgg gggtggtccg ctcgaattca ctcgactgtc gggtgggact gatgtccggc
     4081 tgggagtggg cccgcagcgg cacctcccgc cgggtccggc aggccggagc acagctcgtc
     4141 ctgggcggta tgtgctggcc ttcgatgccg accaactggc cgggcccgct gaaactgctg
     4201 acctcgcggg aacatcggat ttggaaggaa caggcccggg ccctgcccgg acaggtcgcc
     4261 cgcataaccg gcgcacctgt cgcgatcgcc tctcatgtgg gaccaattac gggggaaact
     4321 cccctgattc cggggttgcg atggccgacg accatgatcg gtgaatccca gatctgcgac
     4381 cgcgacggca tggttctcgc ccgcctctcg gaagaagacg gcgagggaca tgtctcggcc
     4441 gaagttcaac tcggggaacc agcaccggcc gagagcatcc ccgacagcta ctggatcccc
     4501 cgaatga
//
LOCUS       DAOTIP010000069         4493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_22949_length_4493_cov_2.489184, whole genome shotgun sequence.
ACCESSION   DAOTIP010000069 DAOTIP010000000
VERSION     DAOTIP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22949_length_4493_cov_2.489184"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2089
                     /locus_tag="P5138_02110"
     CDS             <1..2089
                     /locus_tag="P5138_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV59400.1"
                     /translation="RHFEKPVPLSPLIAIDRERCILCYRCARFSQEVSEDGQLQLLER
                     GADSYIGTFDDRPYIAPFQGNITELCPVGALTSYTYRFQARPWDIEQGGSVCTLCPSQ
                     CNVSFTIRDEQVKRVLDRENDAVDDGWLCDRGRYGFQAMYSQDRVMNPLRYVDGHPVP
                     AQWAEGLEIASIGLAAAEGKVAALVGDASNEEAFILQDLIRNGLGSNDIDSRQARTIA
                     REQRVRLDDPATQARVADIDHADVVLVLGTDPIHSAPAFDLRVRKAVRRNCAALAVAT
                     ERPSALDGGATEVDRIKPGQTAGYLAGLLEQVRSGSSEGIAGLLRDSSHTVVIWGERI
                     GRGESGPAAIESLLELAEALDLAGQEESGMIEIPDVANARGLREVGCLPDAGPGLSGL
                     EAGRDVDAIKDGLISGDVRAIVLYGVDPLRDFPDTDGWRRALEAADFVIHFTMASTDT
                     TEFADVVFPLESHAEKDGTITITEGRLQRVRPAAARPGDIRGGWHVLAELAAALGHET
                     GIHAVPEALQRITESVPFYSGLTDAEIGGRGIRWQERPAAQAWNEAVSVGGPDGTPGE
                     VRPAGSPTDAAPADGLVLGTYRDLWANSTTDLSPALSYLIPAQRLEVSVADAERLGLS
                     KGDEVAVKSGDTEVTAFVAIRAALPEGTCFLMEGTQQGNANVFANGRPAIVEIGKATP
                     ALEVVAGNGNGGE"
     gene            2089..3105
                     /gene="nuoH"
                     /locus_tag="P5138_02115"
     CDS             2089..3105
                     /gene="nuoH"
                     /locus_tag="P5138_02115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRV59401.1"
                     /translation="MPDLVLGLPLADTNFVDATWIMIVKSLVIFIVILSLVPLLLLGE
                     RKLLGRFQQRYGPNRVGPYGMMQPLADVGKLLSKQAFRPKGAVPLLYELAPALTIISA
                     VLTLAILPFGDVQGDVGFYGIDVPIGILYFFAFSSIAFYGLLLGGWASGSKYSFLGAM
                     RSAAQLISYEIAMGLSLLGVVMMAGSLSLVDIVKAQGEIWYVVPQIVGFLIFLVAGFA
                     ETNRPPFDLPEADAELVAGYGTEYGGMRFGSFMFAEYMEMLIVSGIASTMFLGGWMGP
                     GPPGLDPVWMLLKMIILTVFFIWIRATLPRFRYDQLMSFGWKILLPIATLNVLVTAVL
                     VVVT"
     gene            3102..3632
                     /gene="nuoI"
                     /locus_tag="P5138_02120"
     CDS             3102..3632
                     /gene="nuoI"
                     /locus_tag="P5138_02120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRV59402.1"
                     /translation="MSIRPDEIKVVPRGPEPGGMGGAYRAFGETLRGLRTTMGRLFEK
                     PVTIQYPEEKTPVYPRFRGRHKLHKFEDSGLEKCVGCSLCAAACPAECIRVVAAENDP
                     ENRVSGGERYAAVYEINLARCIFCGYCEIACPFDAITMGHDYEMADYNRSDLIFTKEM
                     LLAESLERTPLRGEGE"
     gene            3632..4174
                     /locus_tag="P5138_02125"
     CDS             3632..4174
                     /locus_tag="P5138_02125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRV59403.1"
                     /translation="MEQIAFFVGAIGALGAAFAVVIVKNPFYSVLALVIHLVFLAGIF
                     LLLMAQFLAAALIVVYAGAVMVLYVFVAAYVGGIEEPKWDSSPELRIVGPILAVALFI
                     AISIAVLGSGLSALGTPGPDDVPSGFGTPEAVGHLMMEDFLIAFEAASLLLLIAAVAA
                     IVLAGRRREDRKKLPGGEGA"
     gene            4174..>4493
                     /gene="nuoK"
                     /locus_tag="P5138_02130"
     CDS             4174..>4493
                     /gene="nuoK"
                     /locus_tag="P5138_02130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRV59404.1"
                     /translation="MDIEWYLVLSALLFATGFIGVMVRRNPVVILLCLELMLSAGALA
                     LLAFSRMLGNQDGQVFVLIVLVVAACEVVVGLGLVVAMYRRRLPLDVDEINEFWYEKY
                     RVGVV"
BASE COUNT          694 a         1521 c         1446 g          832 t
ORIGIN      
        1 gcgccacttc gagaagccgg tcccgctctc gccgctgatc gcgatcgacc gcgagcgctg
       61 catcctctgt taccgctgcg cccgcttcag ccaggaagtc tccgaagacg gccagcttca
      121 gttgctcgag cgcggcgccg actcctacat cggcaccttc gacgaccggc cctacatcgc
      181 ccccttccag ggcaacatca ccgagctctg cccggtcggc gcgttgactt cctacaccta
      241 ccggttccag gcccggccct gggacatcga gcagggtggc tcggtttgca ccctctgccc
      301 gagccaatgc aacgtgagct tcacgatccg cgacgaacag gtcaagcgcg tgctcgatcg
      361 tgaaaacgac gcggtcgacg acggctggct ctgcgaccgc ggccgttacg gcttccaggc
      421 gatgtactcc caggaccggg tcatgaaccc gctgcgatac gtcgacggcc acccggtccc
      481 cgcgcagtgg gctgagggcc tcgagatcgc atcgatcggc ctcgcggccg ccgaagggaa
      541 ggtcgccgcc ctggtcggcg atgcctcgaa cgaggaagca ttcatcctgc aggacctgat
      601 ccgcaacggt ctcggctcga atgacatcga ctcccgccag gcccggacga tcgcccgcga
      661 acagagggtc aggctcgatg acccggctac ccaggcccgg gtcgccgaca tcgatcacgc
      721 cgacgtggtg ctcgtgctcg gcaccgaccc gatccactcc gcgccggcgt ttgacctgcg
      781 ggtacgcaag gcggtccgcc gcaactgcgc cgcgctggcg gtcgcaaccg agcggccttc
      841 ggccctggat ggcggcgcga ccgaagtaga ccggatcaag cccggccaga ccgccggtta
      901 tctggccggc ctgctggaac aggttcggtc cgggtcgagc gaaggcatcg ccggcctgct
      961 cagggattcg agccacacgg tcgtgatctg gggcgagcgg atcggtcgcg gtgagagcgg
     1021 cccggcggcg atcgagtcac tgctggagct tgccgaagca ctcgacctgg ccggtcagga
     1081 ggaatccgga atgatcgaga ttccggatgt cgccaacgcc cgcggcctgc gcgaggttgg
     1141 ctgcctgccc gacgccggtc cgggactctc cggtctggaa gccggacgtg acgttgacgc
     1201 gatcaaggat ggtctgattt ccggtgacgt ccgggcgatc gtcctctacg gagtcgaccc
     1261 gctgcgtgat ttcccggata ccgacggctg gcgtcgcgcc ctcgaggccg ccgacttcgt
     1321 gatccacttc acgatggcct cgaccgatac caccgaattc gcggacgtcg tctttccgct
     1381 cgagtcgcat gccgagaagg acggcaccat caccatcacg gagggtcgcc tgcagcgggt
     1441 ccgtccggcc gcagcccgcc ccggagacat ccgcggaggc tggcacgtcc tcgccgagct
     1501 cgcagccgcc ctcggccacg agaccggaat ccacgccgtg ccggaggcgc ttcagcgcat
     1561 cacggagtcc gtccccttct attccggcct caccgacgcc gaaatcggag gacgcggcat
     1621 ccgttggcag gaacgtcccg ccgcacaggc ctggaatgag gcggtatccg ttgggggtcc
     1681 cgacggaact cccggcgaag tccggccggc aggatcccca acggatgccg cccccgccga
     1741 tggcctggtt ctcgggacat atcgcgacct gtgggcgaac agcaccacgg acctgagtcc
     1801 cgccttgagt tatctgatcc cggcacagag gctcgaagtg tcggtggctg atgccgaacg
     1861 cctgggattg agcaagggtg atgaggttgc cgtgaagtcg ggggataccg aggtgaccgc
     1921 gttcgtggcg atccgggcgg ccctgcccga agggacctgc ttcctgatgg agggcaccca
     1981 gcagggcaac gccaacgtat ttgcgaacgg caggcccgcg atcgtcgaaa tcggcaaggc
     2041 gacgcctgcg ctggaagtcg tggccggaaa cgggaacggg ggagagtagt gccggatctc
     2101 gtcctgggcc tgccgctggc cgacaccaac ttcgtcgacg cgacctggat catgatcgtc
     2161 aagtcgctgg tcatcttcat cgtgatcctt tcgctggtcc cgctgctgct gctcggcgag
     2221 cgcaagctcc tcggccgctt ccagcagcgc tatggaccca accgggtcgg cccctacgga
     2281 atgatgcagc ccctggccga cgtcggcaag ctgctctcca agcaggcctt ccggccgaag
     2341 ggtgcggttc ctctcctcta cgagctggcc cccgccctga ccatcatctc ggcggtcctg
     2401 accctggcga tcctgccctt cggcgacgtc cagggtgacg tcggcttcta cggcatcgat
     2461 gtgccgatcg gcatcctcta cttcttcgcc ttcagttcga tcgccttcta cggtctgctg
     2521 ctcggcggct gggcctccgg ctccaagtac tccttcctcg gcgcgatgcg ctccgccgcc
     2581 cagctgatct cctacgagat cgcgatgggg ctttcgctgc tcggcgtggt gatgatggcc
     2641 ggatcccttt cgctggtcga catcgtcaag gcccagggtg agatctggta cgtggtaccg
     2701 cagatcgtcg gcttcctgat cttcctggtc gccggcttcg cggaaaccaa ccggccgccc
     2761 ttcgaccttc ccgaggcgga cgccgagctg gttgccggtt acggcaccga gtatggcggc
     2821 atgcgcttcg gctccttcat gttcgccgag tacatggaga tgctgatcgt ctccggcatc
     2881 gcctcgacca tgttcctcgg cggctggatg ggccccggac cgcccggcct cgacccggtc
     2941 tggatgctgc tgaagatgat catcctgacc gtcttcttca tctggatccg cgccaccctg
     3001 ccgcgatttc gctacgacca gctgatgagc ttcggctgga agatcctgct cccgatcgcg
     3061 actctcaacg tgctcgtgac cgccgttctg gtggtggtca cgtgagcatt cgtcccgacg
     3121 agatcaaggt tgttccgcgc ggtcccgagc cgggcggcat gggcggcgcc tatcgtgcct
     3181 tcggcgagac cctgcggggt ctccggacca ccatgggccg gctcttcgag aagccggtga
     3241 caatccagta cccggaggag aagaccccgg tctatccgcg attccgcgga cgccacaagc
     3301 tgcacaagtt cgaggattcc gggctggaga aatgtgtcgg ctgctcactc tgcgccgcgg
     3361 cctgcccggc cgagtgcatc agggtggtcg ccgccgagaa cgatcccgag aaccgggtct
     3421 cgggcggcga gcgctacgcc gcggtctacg aaatcaacct cgcccgctgc atcttctgcg
     3481 gctattgcga gatcgcctgc cccttcgatg cgatcaccat gggtcatgac tacgaaatgg
     3541 ccgactacaa ccgctcggac ctgatcttca ccaaggagat gctgctggcc gagtcactcg
     3601 agcggacgcc gctgagggga gagggcgagt agtggaacag atcgccttct tcgtcggcgc
     3661 gatcggcgcc ctcggggccg cgttcgccgt ggtcatcgtc aagaacccgt tctacagtgt
     3721 gctggccctg gtcattcacc tggttttcct ggccgggata ttccttctgc tcatggccca
     3781 gttcctcgcc gccgccctga tcgtggtcta cgccggcgcg gtcatggtgc tctacgtctt
     3841 cgttgccgcc tatgtcggcg gaatagagga accgaaatgg gattcctcgc cggagctcag
     3901 gatcgtcggc ccgatcctcg cagtcgccct cttcatcgcg atctcgatcg cagtgctcgg
     3961 ctccggcctc tctgcgctcg gaacaccggg cccggatgac gttccctcgg gctttggcac
     4021 gccggaagcg gtcggccacc tgatgatgga ggacttcctg atcgccttcg aagcggcctc
     4081 gctgctgctg ctcatagcgg cggtcgcggc gatcgtgctc gccgggcgcc gccgggaaga
     4141 ccggaagaag ctgccgggcg gagagggtgc ctgatggaca tcgagtggta tctcgtcctc
     4201 tcggcgctgc tcttcgcgac cggtttcatc ggggtcatgg tccgccgcaa cccggtggtg
     4261 atcctgctct gcctcgaact catgctgagc gccggagcac tcgccctgct cgccttcagc
     4321 cgcatgctgg gaaaccagga tggtcaggtc ttcgtgctga tcgtgctggt ggtggcggcc
     4381 tgtgaggtcg tggtcggtct cgggctggtc gtcgccatgt acagacgccg gcttccgctc
     4441 gacgtggacg agatcaacga attctggtac gagaagtacc gtgtcggggt cgt
//
LOCUS       DAOTIP010000070         4461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23174_length_4461_cov_3.901952, whole genome shotgun sequence.
ACCESSION   DAOTIP010000070 DAOTIP010000000
VERSION     DAOTIP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23174_length_4461_cov_3.901952"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /gene="lexA"
                     /locus_tag="P5138_02135"
     CDS             <1..607
                     /gene="lexA"
                     /locus_tag="P5138_02135"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRV59405.1"
                     /translation="KMGYPPTVREIGQAVGLTSSSTVHVHLANLEKAGVLRRDPSKPR
                     ALEVLVGKAKSAGETVRDSAREVVDTVANVASAPSGLPLLGRVSAGPGILAEENIEDY
                     VEVPEMIGGTDGDYVLEVRGESMKNAGIMDGDYVIVHPAPEARNGEIVVALLGDEDAT
                     VKRFYKETDHFRLEPENETMDPIITTEAQVMGKVIGVFRKV"
     gene            complement(915..1286)
                     /gene="rnpB"
                     /locus_tag="P5138_02140"
     ncRNA           complement(915..1286)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5138_02140"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     regulatory      1421..1574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1581..2279
                     /locus_tag="P5138_02145"
     CDS             1581..2279
                     /locus_tag="P5138_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HRV59406.1"
                     /translation="MVRSFRLGKLNIAVAALLILGLALVTLLFGGHKDTAAAASGGIA
                     LSSPSKSTSNSSGSKAVKGTRFGARTLRQGMTGADVKVLKSIVRSKRLLSGALNTTFD
                     RPTTSAVRRFQGSANMNTSGVVNKTTARKMIGSLRTSEASWYGPGLWGNGTACGKVTL
                     RPNTIGLANKNLPCGTRVLIGYRGKFVIAPVIDRGPYIAGRTWDLTKAASDAIGLTGA
                     GVGRVRHAVLSRGK"
     gene            2477..3262
                     /locus_tag="P5138_02150"
     CDS             2477..3262
                     /locus_tag="P5138_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59407.1"
                     /translation="MSANTSPAFGRRTQLAAALAGFVALLAGLAIMAGPGNQADAAPG
                     KAVYLGKASAKRIPLCPQRCSGLAIVSGFQAKAGGIGNAYRVPYIGNITRWRIKLGKP
                     TASQRMFFQDRFGAVPQAAIGVLAKKVQNGKIVYKLRRRSAIQNLNQFMGKTHTFDLK
                     QPIKANKGDYVVLIVPTWAPALSVPEACSTVTVNGKTQMKNPSVCKQFNTNNSWVASR
                     DRKTCNQAVTMKNSQPQIDVNSLASYGCRFNGALTYGVRVESR"
     gene            complement(3403..3969)
                     /locus_tag="P5138_02155"
     CDS             complement(3403..3969)
                     /locus_tag="P5138_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRV59408.1"
                     /translation="MTVKTEVSRFHIHDELTAPEESAPILKSIQVGGRQVNKLVGVLA
                     GSPAVLRAYTRMRAELQQGELPQATRHRIALAVAEHRGDAYSVVQYAKTARNAGLGLD
                     EVSRARSFASSDPREQALLTYLKAALEAEGDPPIYLHEEAREAGWDDEQILEALAHVA
                     MGEFQSVMAAAAALPKDQATPRVLPDAA"
     gene            4185..>4461
                     /locus_tag="P5138_02160"
     CDS             4185..>4461
                     /locus_tag="P5138_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRV59409.1"
                     /translation="MAVQTNTPEDVFEAVKEHDVRFVRLWFTDVLGHLKSFSINSSEL
                     ERAFEQGIGFDGASLTGFNPVEESDMVARPDASTFSLIPWRPGGTRRC"
BASE COUNT          794 a         1430 c         1412 g          825 t
ORIGIN      
        1 caagatgggc tatccgccca cggtcaggga aatcggccag gcggtggggc tcacctcctc
       61 ctcgaccgtg catgtccatc tggcgaacct cgaaaaggcc ggcgtgctgc gccgtgaccc
      121 gagcaagccg cgggcacttg aagtgctggt cggcaaggca aagagcgccg gcgaaaccgt
      181 gcgggacagc gcccgcgagg tcgttgacac ggtcgcgaac gtggcctccg cgccgtccgg
      241 attgcccctg ctcggccggg tttcggcagg tccggggatt ctcgccgaag agaacatcga
      301 ggattacgtc gaagtgccgg agatgatcgg gggtaccgat ggcgactacg tgcttgaggt
      361 tcgcggcgaa agcatgaaga acgccggcat catggacggc gattacgtga tcgtccatcc
      421 tgcgccggaa gccaggaacg gagagatcgt cgtcgccctg ctcggggacg aggacgcaac
      481 cgtcaagcgc ttctacaagg aaacggacca cttccgtctc gaaccggaaa acgagacgat
      541 ggatccgatc atcaccaccg aggcccaggt aatgggcaaa gtaatcggag ttttccgcaa
      601 ggtctgaacc cgggcggccc ccagaagtcc tggcaccgcg cccgggagcc gctcacggta
      661 ggtgcctact cggcggcccg ccctggaggc tcctgacatc cactcgctga aggtcgggct
      721 cggtagcgca cggtgtaaca agttttgtgc gttgtctgtg ggaaatgtgt ttcgccttcc
      781 atgggccgat gccgggcggg gtggtgacgg aggcttgtga ccggcggggt gccaaacctg
      841 cgtcaccttg gttccgaatc agggctcagc caacccggcc tcgtgcaagt agaagaggcc
      901 ccgggtgggg cctcttcctt agcgggtctg taagccggat cctgtcttga acgaccatct
      961 atctcaccgg ttgatcggac cggtgggcca ccgcagtgac ctgcgaccta cctggatctc
     1021 gccgggccgg gtcgacgatc ctgtttggtc ttgcaccgga cggggtttac ctggccgccg
     1081 tgtcaccacg acgccggtgc gctcttaccg caccttttca cctttgcctg atcgcgactc
     1141 acgtgtggtc gcgccatcgg ccgtgtattt ctgtggcact ttcccgcggg tttccccggg
     1201 tgggcgttac ccaccgtcct tgcccttcgg tgtccggact ttcctcgagg agttttctct
     1261 ctccccgcag ccgtccgacc cacaaaggca attgtagcgg cgtgttccgg gtcagcagac
     1321 tggattgaaa ggcgctgcaa tggcgaactc gcagttcata aagagtgtgc gtaagtcaca
     1381 gagcgaaatg tgcatgctcc ttgagcgttt gctaatgttc gctcaccttc tgctgaatct
     1441 ccgcctcaag atcgcggaga acgggggacc caaaggacaa cggtccaggg gcgaatcact
     1501 ggcatctgcc aggtagcgct gctctttcag cgcgagcccg acagctaacc ccgcaagcct
     1561 tacgaaagag agaagaagga atggttcgca gcttccggct gggaaaactg aatatcgcgg
     1621 tcgccgccct gctcatcctg gggctggccc tggtcacgct gctgttcggc ggacacaagg
     1681 acaccgccgc ggcagcttcc ggcggtatcg cgctttcttc cccgagcaag agcaccagca
     1741 acagctcggg gagcaaggcc gtgaagggca cccgctttgg tgcccgcacc ctccggcagg
     1801 gcatgaccgg cgccgacgtg aaagtgctga agagcatcgt ccggtccaag cggcttcttt
     1861 ccggcgcgct caacacgacc ttcgaccggc cgaccaccag cgccgtccgt cgtttccagg
     1921 ggtcggcgaa catgaacacg agcggcgtcg tgaacaagac caccgcccgc aagatgatcg
     1981 gttcgctccg gacctcggaa gcctcctggt acggaccggg gctctggggc aacggcaccg
     2041 cctgcggcaa ggtgacgctg cgtcccaaca cgatcggcct tgccaacaag aaccttccct
     2101 gcggaacccg cgtgctgatc ggataccgcg gcaagttcgt gatcgccccg gtgattgacc
     2161 gcggcccgta catcgccgga cgcacctggg atctcaccaa ggccgcctcc gacgcgatcg
     2221 gccttaccgg cgccggcgtc ggccgggtca ggcacgccgt cctcagccgc ggcaagtagt
     2281 caggacctcg ccgccttcgg ttgttcgctc tgagccgggc aggcttttcg cactgccggc
     2341 cgggtaaccg ggccgcgacg gtttctttgc agggaaaaac cgatccatcg cgggttttcg
     2401 gctgtttgta ccgtgacccg ccgcgccatc aaggaattcg cgccgcgaga cttaacacaa
     2461 ggagctagtg aaactgatgt cagcaaacac ctctccggcc ttcggccgcc ggacccagct
     2521 tgccgccgcc ctcgccgggt tcgtcgccct gttggccggc ctcgccatca tggctggtcc
     2581 gggcaaccag gccgatgccg ccccgggcaa ggccgtctac ctcggcaagg cgagcgccaa
     2641 gcgcatcccg ctctgccccc agcgctgttc cggactcgcc atcgtcagtg gcttccaggc
     2701 caaggctggt ggaatcggta acgcctaccg ggttccctac atcggaaaca tcacccggtg
     2761 gcgcatcaag ctcggcaagc ccacggcgag ccagcgcatg ttcttccagg accggttcgg
     2821 tgccgtgccg caggccgcga tcggcgtgct ggccaagaag gtgcagaacg gcaagatcgt
     2881 ctacaagctc cgccgccgct ccgccattca gaacctgaac cagttcatgg gcaagaccca
     2941 cacctttgac ctgaagcagc cgatcaaggc gaacaagggc gactacgtgg tgctgatcgt
     3001 cccgacctgg gctccggcgc tttcggtgcc cgaggcctgc tcgacggtca ccgtcaacgg
     3061 caagacgcag atgaagaacc cttcggtctg caagcagttc aacacgaaca acagctgggt
     3121 tgccagccgc gaccgcaaga cctgcaatca ggccgtgacc atgaagaact cgcagccgca
     3181 gatcgacgtg aacagtctcg cgtcgtacgg ttgccggttc aacggtgcgc tgacctacgg
     3241 cgtccgggta gagagccgct agcggcacca ccttcagttt cgagaggtac gaaaaaaagg
     3301 acggccccgg atcggggccg tccttttgtt agggggagag gctggtcagg ggagagagga
     3361 cagcctcgtc cgggacctgg gagagggtcc ctttggcgtt gatcaggcgg cgtcgggcag
     3421 aaccctcggg gttgcctgat ctttcgggag ggccgctgct gcggccatca cgctctggaa
     3481 ttcgcccatc gccacgtggg cgagggcctc aaggatctgc tcgtcgtccc atccggcctc
     3541 gcgggcttcc tcgtggaggt agatcggcgg atcaccttcc gcctcgaggg cggccttgag
     3601 ataggtgagg agcgcctgct cgcgcgggtc ggaagaagcg aagctgcggg ccctcgagac
     3661 ttcgtcgagg ccgagcccgg cgttgcgcgc cgtcttcgcg tactggacca cgctgtaggc
     3721 gtcgccgcgg tgctcggcca cggcaagggc gatcctgtgg cgggtggcct ggggcaactc
     3781 gccctgctgc agttcggccc gcatcctggt gtaggcgcgg agcacggccg gtgagccggc
     3841 gaggacgccg accagcttgt tcacctggcg gccacccacc tggatgctct tgaggattgg
     3901 cgcgctctct tcgggagcgg tcagttcgtc gtggatatgg aatcggctga cttctgtctt
     3961 tacggtcacg atgtcacgct tcccagtgtt tcttgaagtg agggcccgca aacgccttta
     4021 tctgtcgttt agcgggccat tactttactt ttagctagtt acttaccgca agtaagttat
     4081 accccctgcg gcggcgtctt gcaagcccct tcgcgtatcg tttcggcctg aggtacctcg
     4141 atcccggctc gcaagccatt tatttcgccc ctccaaggag tccgatggca gtacagacca
     4201 ataccccgga agacgtcttc gaagcagtca aggaacacga cgtccgtttc gttcgactct
     4261 ggttcaccga cgtcctgggg cacctgaaga gcttttcgat taattcgagc gaactcgagc
     4321 gagccttcga gcaggggatc ggattcgacg gggcatccct gaccggtttc aacccggtcg
     4381 aggagtccga catggtcgcc cggcctgacg cttccacctt cagcctgatt ccctggcggc
     4441 cggggggcac gcggcgctgc t
//
LOCUS       DAOTIP010000071         4446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23270_length_4446_cov_3.200410, whole genome shotgun sequence.
ACCESSION   DAOTIP010000071 DAOTIP010000000
VERSION     DAOTIP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23270_length_4446_cov_3.200410"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /gene="recA"
                     /locus_tag="P5138_02165"
     CDS             <1..852
                     /gene="recA"
                     /locus_tag="P5138_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRV59410.1"
                     /translation="EAQKRGGVCAFVDAEHAIDPVYARRIGVNTDELLVSQPDYGEQA
                     LEIVDVLTRSGAVDVVAVDSVAALTPRAELEGQMGEVTVGLQARLMSQALRKLAGNLN
                     RANTLCLFTNQIREKIGVSFGSPETQPGGRALKFYSSQRLDIRRIETLKDGTEAVANR
                     VRVKVVKNKVAAPFKQAEFDIEYGLGISREGALIDLGMEHDLVQKSGSFFSYGETRLG
                     QGRNNSKQFLTENPDIAQEIEDRILANLGISRDGSPIESSAPEGVDAETGEVIEPAGN
                     DAKLKAA"
     gene            903..1367
                     /locus_tag="P5138_02170"
     CDS             903..1367
                     /locus_tag="P5138_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRV59411.1"
                     /translation="MSDEWLAKALAAISRKDRTEAEMREWLVDREVESDEIERVIGYL
                     TENLALDDRRFAFAYASDKRELSGWGKDRIREVLLQRGVDRYLVEQALDQPGPGVGQA
                     ESEVERAVRVLNERGADLSDDRGRQRALGLLARRGYEAEEAYAAIRIASRAA"
     gene            1428..2963
                     /gene="rny"
                     /locus_tag="P5138_02175"
     CDS             1428..2963
                     /gene="rny"
                     /locus_tag="P5138_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRV59412.1"
                     /translation="MALDSIIPAWLRNRSPEPEAGQGAGESRNGAVPQSPETERRAGQ
                     DGVSPRDADSPSPDLDSPPPREGLLSAEAELRARREEIVRMEERALRDLESARTRLRE
                     ADRRTEIAEERERNLDRAEEEIEAAREARLAELEQVSGLSRTEARKLLLKEVEEEAAH
                     QAGLTVRRIEEEARLDAERRARGILAVAMQRLASGQSIESNTQVIHLPNEEMKGRIIG
                     KDGRNIRALENLTGVDIIIDETPETVVVSSFDGVRREVGRLTLEKLIEDGRIHPASCE
                     AAYEEASARVDDLMVEAAESALLDARVTGLHPELVRLLGRLRFRTSYSQNVLSHLVEC
                     SNLAALMAAELGASVEVARRAALLHDVGKAVSHEVEGTHAAVGARLARRYEEVDAITH
                     AIEAHHNEIEPVTVEAVIVQAADALSGARPGARGNSMEQYVSRLGDLEEIALRQAGVK
                     KVFAMQAGREIRIMVDPGQVDDDATALISHEIAAAIEREMEYPGQIKITVIRELRSTR
                     IAR"
     gene            3030..3305
                     /locus_tag="P5138_02180"
     CDS             3030..3305
                     /locus_tag="P5138_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59413.1"
                     /translation="MEIKTVFLIFGISLACFAVIVSFVGLRSKNFPSRGALIGLILAA
                     AVLVGGTTTFAVKLSVEEQHDREEGHKAPGEESKDAAAIKIIRADAA"
     gene            3429..>4446
                     /gene="miaB"
                     /locus_tag="P5138_02185"
     CDS             3429..>4446
                     /gene="miaB"
                     /locus_tag="P5138_02185"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRV59414.1"
                     /translation="MKTFHVTTFGCQMNEHDSERMKGMLTSLGYAESDRRDDADLILF
                     NTCSIRENADNRFISHLGEAKRLKGENPERIVGVGGCWAQSVKDELFRRFPFVDVAFG
                     PGQIHRLAEFLNSDQLTAQGYFEFEDFAGHLPAHRARQFQGWLQISQGCNCVCSYCIV
                     PSTRGREQSRDPQELVSEARQLAAEGVREITLLGQNVNSYGRDLPGDSRIRFSDLLAM
                     IDEIDGIDRIRYTSPHPKDMREDVIEAHAGLPSLCEHIHLPLQSGSSPILKKMRRTYT
                     RERYMDRVAMIRERVPDCAITTDIIVGFPGETEADFEQTLEVVEEVGYDGAFTFVYSP
                     RRGTL"
BASE COUNT          847 a         1397 c         1525 g          677 t
ORIGIN      
        1 gaggcccaga agcgcggcgg ggtctgcgcc ttcgtcgatg ccgagcacgc gatcgatccc
       61 gtttacgcga ggaggatcgg cgtcaacacg gacgaactcc tggtctccca gccggattac
      121 ggcgagcagg cgctggagat cgttgacgtg ctgacccgct ccggtgccgt cgacgtggtc
      181 gcggtcgatt cggtcgcggc actgactccg cgggccgagc tggaaggcca gatgggcgag
      241 gtgacggtcg ggcttcaggc caggctcatg tcgcaggccc tgcggaagct ggccggaaac
      301 ctgaaccggg ccaacacgct ttgccttttc accaaccaga tccgcgagaa gatcggggtt
      361 tccttcgggt ccccggagac ccagccgggt ggccgggcgc tgaagttcta ctcctcccag
      421 cgactcgaca tccgccggat cgagaccctc aaggacggca ccgaggcagt ggcgaaccgg
      481 gtgcgcgtca aggtcgtcaa gaacaaggtc gcagccccct tcaagcaggc cgagttcgac
      541 atcgaatacg gcctcgggat ctcccgggag ggcgccctga tcgacctcgg catggagcat
      601 gacctggtcc agaagtcggg ttccttcttc tcctatggcg agacgcgcct cgggcagggc
      661 cgcaacaact cgaagcagtt cctgaccgag aaccctgaca tcgcgcagga gatcgaggac
      721 aggatcctcg ccaacctcgg catctcgcgg gacggttcac cgatcgaatc ctctgcgccc
      781 gaaggcgtgg acgcggaaac aggcgaagtg atcgagcccg ccggaaacga cgcaaagctg
      841 aaggcagcct gagtccccga tcgttccggg tggattggtg agaacggccc ggatctgcgc
      901 cgatgtctga cgaatggctg gccaaggccc ttgcggcgat cagtcgcaag gaccggaccg
      961 aagccgagat gcgggaatgg ctggtcgacc gggaggtgga atccgacgag atcgagcggg
     1021 ttatcggcta cctgaccgag aacctggccc tggatgaccg gcgcttcgca tttgcctatg
     1081 ccagcgacaa gcgggaactc agcggctggg gcaaggacag gatcagggag gtgctgctgc
     1141 agcgcggcgt tgaccgttac ctggtcgagc aagcactcga tcagcctgga cccggtgtcg
     1201 gccaggccga gagcgaggtc gaacgggcgg tgagggtgct gaacgagcgc ggtgccgacc
     1261 tgagcgatga ccgcggaagg caacgtgcac tggggctgct cgcgaggcgc ggatacgagg
     1321 ctgaagaggc ctacgcggcg atcagaatcg cctcccgcgc agcctgaagc cgcacggcaa
     1381 aggcccctca ggggacgttt ggggcgtcgg ctacccttga aggaggcatg gcgctcgact
     1441 cgatcatccc ggcctggctg cgcaaccggt ccccggaacc ggaggccggg caaggggcag
     1501 gggagtcgcg gaacggggcg gtcccccaat caccggagac cgaacgacgc gccgggcagg
     1561 atggggtttc cccgcgggat gcggactctc cgtcgccgga cctcgactca ccgccgcccc
     1621 gggagggcct tctctcggcc gaggccgagc tcagggcgag gcgggaggaa atcgtacgga
     1681 tggaggagag ggcgctccgt gacctcgagt cggcccggac ccggttgagg gaagcggacc
     1741 gcaggacgga gatcgccgag gaacgggaac gaaacctcga ccgcgccgag gaggagatcg
     1801 aggccgccag ggaagcccgg ctggccgaac tggagcaggt ttcggggctg agccgcaccg
     1861 aagccaggaa gctgctgctc aaggaagtcg aggaagaagc cgcccatcag gcgggtctca
     1921 ccgtgagaag gatcgaggag gaggccaggc tggacgccga acggagggcg aggggcatcc
     1981 tcgcagtggc aatgcagcgg ctcgcatccg gccagtcgat cgagtccaac acccaggtaa
     2041 tccaccttcc gaacgaggag atgaaagggc ggatcatcgg caaggacggc cgcaacatcc
     2101 gggcgctcga gaacctgacc ggggtcgaca tcatcatcga cgagacaccc gaaaccgtgg
     2161 ttgtctcgag tttcgacggg gttcgccgcg aggtcgggag gctgaccctg gagaagctga
     2221 tcgaggacgg ccggatccat ccggcttcct gcgaagcggc atacgaggag gcgtcggcaa
     2281 gggtggacga cctgatggtc gaggcggccg aatcggcgct gctggacgcc cgggtcactg
     2341 gcctgcatcc ggaactggtg cgactgctcg gaaggctcag gttccgcacc agctacagcc
     2401 agaacgtgct cagccacctg gtcgaatgtt cgaaccttgc ggcgctgatg gcggccgagc
     2461 ttggcgcctc ggttgaagtg gcccggcggg cggcgctgct gcatgacgtg ggcaaggcgg
     2521 tcagtcacga ggtcgaaggg acccatgcgg cagtcggggc caggctggcc cggcgttacg
     2581 aggaagtcga cgcgatcacg cacgcgatcg aggcccatca caacgagatc gagccggtga
     2641 ccgtcgaggc agtgatcgtg caggcggccg atgccctgtc gggcgccagg cccggagctc
     2701 gcggcaactc tatggagcaa tacgtctccc ggctcggcga tctcgaggaa atcgccctcc
     2761 gtcaggcagg cgtgaagaag gtctttgcga tgcaggcggg acgggagatc cggatcatgg
     2821 tcgaccccgg gcaggtcgat gacgatgcga ccgctctcat ctcgcatgaa attgccgccg
     2881 cgatcgaacg ggaaatggag taccccggcc agatcaagat caccgtgatc agggagctcc
     2941 gctcgacgcg tatcgccaga tagcgacacc ccctccgggg gtgccggccc gacgcccggg
     3001 cgccgccgaa atcagtagga ttcgcgccga tggaaatcaa gaccgtcttc ctcatcttcg
     3061 gcatcagcct ggcctgcttc gcggtgattg tcagcttcgt gggcctccgg tcgaagaact
     3121 tcccgagcag gggagcactg atcgggctga tcctggccgc ggcggttctg gtcggcggca
     3181 ccaccacttt cgccgtcaag ctctccgttg aagagcagca tgaccgcgaa gaggggcaca
     3241 aggccccggg cgaagagtcc aaggacgccg ccgccatcaa gatcatccgc gccgacgccg
     3301 cctgagccct ctctcgggtc cggagcgggc atgcccctcc tggtccgtcg cccggcgggc
     3361 tcccccgaag gcccgggagc tacgcccccg cgccgcgctc ccacagctgc ttgtaatctc
     3421 gtaggtcgat gaagacgttt catgtcacca ccttcgggtg ccagatgaac gagcatgact
     3481 ccgagcggat gaaaggcatg ctcacttcgc ttggctatgc ggaatcggac cggcgcgacg
     3541 atgccgatct gatcctgttc aacacctgtt cgatccggga gaacgcggac aaccggttca
     3601 tctctcacct cggggaggcg aagcgtctca agggcgaaaa cccggaacgg atagtcgggg
     3661 tcggtggctg ctgggcccag tcggtgaaag acgagctttt caggcgattt ccgtttgttg
     3721 atgtggcgtt cggtcccggc cagatccacc ggctggccga gttcctcaac tccgatcagc
     3781 tgactgccca gggttatttc gagttcgagg atttcgccgg ccacctgccg gcccaccggg
     3841 cgaggcagtt ccagggatgg ctccagatat cccagggctg caattgcgtc tgctcctact
     3901 gcatcgtgcc ttcgaccagg ggccgcgaac agagccgcga cccgcaggaa ctggtcagcg
     3961 aagcccgtca gctcgctgcc gaaggcgtcc gggagatcac cttgctcggc cagaacgtca
     4021 actcctacgg ccgcgacctt cccggggaca gccggatccg cttctccgac ctgctggcga
     4081 tgatcgacga aatcgacggg atcgaccgga tccggtacac gagcccgcat ccgaaggaca
     4141 tgcgtgaaga cgtgatcgaa gcgcatgccg gcctgccttc cctctgcgaa cacatccacc
     4201 tgccgctgca gtcgggttcc agcccgatcc tcaagaagat gcgtcgcacc tacacccgcg
     4261 agcggtacat ggaccgggtc gcgatgatcc gcgagagggt tccggattgc gcgatcacca
     4321 ccgacatcat cgtcggcttc ccgggcgaga ccgaggccga cttcgagcag acgctcgagg
     4381 tggtcgagga agtgggatac gacggcgcct tcaccttcgt ctactcgccc aggcgtggca
     4441 ccctgg
//
LOCUS       DAOTIP010000072         4417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23457_length_4417_cov_3.343191, whole genome shotgun sequence.
ACCESSION   DAOTIP010000072 DAOTIP010000000
VERSION     DAOTIP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23457_length_4417_cov_3.343191"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..709
                     /locus_tag="P5138_02190"
     CDS             77..709
                     /locus_tag="P5138_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV59415.1"
                     /translation="MPRINGQLLVYIGVGIVLVLVGLNSIRGEKSESATFGVNSGRAG
                     SGSVSGGNSPAADGGFSTSAATRKLIVDVAGAVKKPGVYRFSQGARVIDAIARAGGVT
                     GRAMTGAINRAATLNDGQQVVVPSSVAGGEGAGDGSALPAASAPGAPVSLGTATQAQL
                     EEIDGIGPVTARKILEFRDSQGGLGSIDELDQISGIGPVTMESLRAALSP"
     gene            706..3036
                     /locus_tag="P5138_02195"
     CDS             706..3036
                     /locus_tag="P5138_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRV59416.1"
                     /translation="MKLTADWRLVAAVGGAAGMALSPFLDPGGSWPIALLAAVLATVI
                     FVRRGRLVGVGLILSGLATAVAGLLVGAARVDSIDGGALREAPGPVALSGFVTSLPSQ
                     SRGTFRFDLETGSGRVLVETSSPRADTLLPGRGVLVRGSLQEAPDWMASDLKRRGIEM
                     IVRAGRISPDGRRRGGLAGLIDRMRDNALRALSIAVPRRESALSRGFVLGDDSDVDGR
                     TTEDFRRSGLSHLLAVSGQNVVLLALLAVPFLALLGIGPRGRLAGIAGLILIYVPLAG
                     GGPSIQRAGVMGLAGLAALAATRAPSRLYALALAALVTLALNPRATSDVGWQLSFAAV
                     VGIMLMARPLHRRLAPMIGDEGWRRSLCEGAAVTIAATVATAPLIAFHFERLPVATLA
                     ANLAAMPAVAPAMWLGMVGIAVGQVSPVLAAPFNLAGSVFLAWIAQVAEWFGRPAWAV
                     VEADPGSPFVLVLVSLVLALATLAVFRLWRVSPNPVRAGSWLPAALVVLLGLIVLLPG
                     ILGTGRRHLDQPPEGGARVEVLDVGQGDAILLRPSDADPILIDGGPPDGDLTGALESA
                     GVDHLAAVLVTHPDLDHYGGLLDLFGTVPADRLLFDKAPPELLALARRSGARFLRVSQ
                     GERFKDGDIRIEILWPPAEDPAAAGAEIGPTSYGSSPTNLRSIVALFEWRDFRMLLTG
                     DAEAETVPMESGPIDVLKVSHHGSEDSGLPQLLEESRPRLAIISVGKDNRYGHPVPDI
                     LNELSGDGIPTLRTDEDGTVSVVLGDGPGYRVETGQ"
     gene            3033..4049
                     /gene="holA"
                     /locus_tag="P5138_02200"
     CDS             3033..4049
                     /gene="holA"
                     /locus_tag="P5138_02200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRV59417.1"
                     /translation="MSGRVDQPPRRRGIGENVGVKPAYLIHGSDSAKIDQARARLRQR
                     AEAESGAGGLEVFEPADGKGSPDADALAASIGSLSLMPGRRYLLADRIEKWGKNQTAT
                     VAEAIREAPDETTVVLIARGKVPAGIAEAVKKVGGETLAYEAPEGARLPVYLVESAAA
                     RDFELTPEAARYMIARMGESLPRLGAELDRLALWAGAGGSVDVEDLQEMVADTSETSN
                     FALGDAVISGDRKEVIAVAERLLAQGATAGSTVYTTSTSVRRAHKALVRLEAGEDPAR
                     LERELGVPPFVARRLINSLSGTTVEAMREAAIALAELEVWTRGGAEYPDELALDLALL
                     AATE"
     gene            complement(4052..>4417)
                     /gene="rpsT"
                     /locus_tag="P5138_02205"
     CDS             complement(4052..>4417)
                     /gene="rpsT"
                     /locus_tag="P5138_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRV59418.1"
                     /translation="GGFKGVELGVRGRVQAGNGIVAGRSSAEIPREHTEKIMANIASQ
                     KKRILRTERERVENRRLTSAIKTYFNRLEKAAEGGDADAVQAEHRALVSRIDKAVQKG
                     AIHRNNGARKKARAARIAA"
BASE COUNT          657 a         1427 c         1570 g          763 t
ORIGIN      
        1 gcgcttacgg atcgcccgtt cggaatcgtt gcacccgtcg agccacgggt gagctttttc
       61 gtggcaggtt cgaggcatgc cccgaatcaa cggacaacta ctggtctata tcggggttgg
      121 catcgtcctg gttctcgtcg ggctgaactc gattcggggt gagaagtccg agtccgcgac
      181 cttcggggtc aactccggca gggcggggag cgggtcggtt tccggcggca actcaccggc
      241 cgctgacggc gggttttcga cgagcgccgc cacgcgaaag ctgatcgtcg acgtggccgg
      301 ggcggtgaag aagcccgggg tctaccggtt cagccagggc gcccgggtga tcgacgcgat
      361 cgccagggcc gggggtgtca ccggcagagc gatgaccgga gcgatcaacc gggcggcgac
      421 actcaacgac ggccagcagg tcgtggtgcc ctcgtctgtg gcgggcggcg aaggcgcggg
      481 cgacggatcg gctcttcccg ccgcctcggc gcccggggct ccggtcagcc tcggaacggc
      541 cacccaggcg caactcgagg agatcgacgg gatcgggccg gtcacggcgc ggaagatcct
      601 cgagttccgt gactcgcagg gagggcttgg atcgatcgat gaactcgacc agatcagcgg
      661 catcggtccg gtcacgatgg agtcgctccg ggcggcgctg tcgccgtgaa actcaccgcc
      721 gactggcggc tggtggcagc ggtcggcggt gcggcgggca tggccctgtc gccgtttctc
      781 gatcccgggg gaagctggcc gattgccctt ctggcggccg ttctggcgac ggtgatcttc
      841 gtccggcgtg gccgactggt cggcgtcggc ctcatcctga gcggattggc gaccgcggtc
      901 gccggactgc tcgtcggtgc cgcgagggtg gactcgatcg atggcggggc tctgcgggaa
      961 gcccccgggc cggttgcact ttcgggattc gtcaccagcc tgccttcgca gtcgcgcgga
     1021 accttccgct tcgacctcga gaccggctcc ggcagagtcc tggtcgagac ctcatccccg
     1081 cgggcggaca cactgttgcc gggccggggc gtgctggtcc gcggaagtct ccaagaggca
     1141 cccgactgga tggcatccga cctgaagaga cgtggaatcg agatgatcgt ccgcgccggc
     1201 cggatatcgc cggacggccg gaggcggggc gggctggccg gcttgatcga ccggatgcgt
     1261 gacaacgccc tgcgtgctct ttcaattgcc gttccccgac gtgagtcggc tctttcccgc
     1321 ggcttcgtgc tgggtgacga cagcgacgtg gacggccgca ctaccgagga tttccgccgc
     1381 tccggcctca gccatctact tgccgtgtcc gggcagaacg tggtcctgct ggccctgctt
     1441 gcggtcccgt ttctcgcatt gctcggaatc gggccccggg ggcggctggc cgggatcgcc
     1501 ggactgatcc tgatctacgt tcctctggcc ggtggcggtc cgtccattca gagagccggg
     1561 gtcatgggcc tggccgggct ggccgcactc gcggcgaccc gggcgccttc gaggctctac
     1621 gcgcttgcgc tggcggccct ggtcaccctt gccctgaatc ccagggccac ttcggacgtc
     1681 ggctggcaac tcagcttcgc ggcggtggtc gggatcatgc tgatggcgag gccgttgcat
     1741 cgaaggctcg ccccgatgat cggggacgaa ggctggcggc gaagcctttg cgaaggagct
     1801 gctgtcacca tcgcggccac ggtcgcgacc gcaccgctca tcgccttcca cttcgagcgg
     1861 ctgccggtgg cgaccctcgc cgcaaacctc gcggccatgc ccgcggtggc accggcgatg
     1921 tggcttggga tggtggggat cgcggtcggt caggtcagtc cggtactcgc cgccccgttc
     1981 aacctcgccg gctcggtttt tctcgcatgg atcgcccagg tcgccgaatg gttcggacgt
     2041 ccggcctggg ccgtggtcga agcagatccc ggaagtccat tcgtcctcgt tctggtctcc
     2101 ctggtgctgg cactagcgac gctcgcggtc tttcgccttt ggcgggtcag cccgaatccg
     2161 gtcagggccg ggagctggct gccggcggcc ctggtggtcc tgctcggcct gatcgttctc
     2221 ctgccgggga tcctcggaac cggccgacgg cacctggacc agccgccgga aggaggggcg
     2281 cgggtggaag ttctcgacgt cggacagggg gacgcgatcc tcctgcgacc gtccgatgcc
     2341 gacccgattc tgattgacgg cggtcccccg gacggcgatc tgaccggtgc cctcgaatcc
     2401 gccggtgtgg atcaccttgc tgccgttctg gtcactcacc ccgatctcga ccattacgga
     2461 ggtctcctcg atctcttcgg gaccgtcccg gcagaccgtc tgttgttcga caaggctccg
     2521 cccgaactgc tggcactggc tcgcaggtcg ggggccaggt tcctccgggt cagtcagggc
     2581 gaacggttca aggacggcga catacggatc gagatcctct ggccgccggc ggaggatccc
     2641 gctgctgcgg gggcggagat cggaccaacc tcctacgggt ccagtcccac caatctcagg
     2701 tcgatcgtcg ccctcttcga gtggcgggat ttccggatgc ttctgactgg tgacgcggaa
     2761 gccgagacgg tgccgatgga gtccggtccg atcgacgtgc tcaaggtctc ccaccacggc
     2821 agcgaggatt ccggcctgcc gcagctgctc gaagaatccc ggccccgtct ggcgatcatc
     2881 tcggtcggaa aggacaaccg ctacggccat ccggtcccgg acatcctgaa cgagctatcg
     2941 ggggacggaa tcccgaccct gaggaccgac gaggacggaa cagtctcggt cgttctcgga
     3001 gacggtcccg gttaccgggt agagaccggt caatgagcgg ccgcgtcgat caaccgccgc
     3061 gccgccgggg gatcggcgaa aatgtggggg tgaaaccggc ttacctgatc cacggcagcg
     3121 acagcgccaa aatcgaccag gcgcgcgccc ggctcaggca acgggccgag gccgaaagcg
     3181 gagccggtgg actcgaggtc ttcgaaccgg ccgacggcaa ggggagcccc gacgcagatg
     3241 cgctggctgc ctcgatcggg tcgctgtcgc tgatgccggg gcgccggtac ttgctcgccg
     3301 accggatcga gaaatggggc aagaaccaaa cggccaccgt ggctgaggcg atccgggaag
     3361 cccccgacga gaccaccgtg gtcctgattg cccgggggaa ggttccggcc gggatcgccg
     3421 aggccgtgaa gaaggtcggg ggagagaccc tcgcctatga ggcccccgaa ggtgcccgcc
     3481 tgccggtcta tctggtcgag agcgcggctg cgcgggactt cgagctgact ccggaagcgg
     3541 cccggtacat gatcgcccgc atgggcgaaa gcctgccccg gctcggtgcg gaactcgacc
     3601 ggctggcgct atgggccgga gccggcggga gcgtcgatgt cgaggatctg caggagatgg
     3661 tcgcggacac ctcggagacc agcaacttcg ccctggggga cgcggtgatc tcgggcgacc
     3721 ggaaggaagt gatcgccgtg gcggagcgat tgctcgccca gggagcgaca gccggaagca
     3781 cggtctacac gacttcgacc tcggttcgcc gggcccacaa ggctctggtc agactggaag
     3841 ccggggagga cccggcgcga cttgaacgcg agctcggggt accgccgttc gtggcccggc
     3901 gtctgatcaa ctcgctttcc ggcaccacgg tcgaggcgat gcgggaagcg gcgatcgccc
     3961 tggcggaact ggaggtctgg acccgaggcg gagcggaata tccggacgaa ctggccctgg
     4021 acctggccct gctggctgct acagagtgaa gctaggcggc gatacgggca gcgcgagctt
     4081 tcttccgggc gccgttattg cgatggatcg cgcccttctg gaccgccttg tcgatgcggg
     4141 agaccagagc gcgatgctcg gcctggaccg cgtcggcgtc gccgccttca gcggcctttt
     4201 cgaggcggtt gaagtaagtc ttgatcgccg aagtcaggcg ccggttctcg acgcgctcac
     4261 gctcggtgcg aaggattctc tttttctgtg aggctatgtt ggccatgatc ttttcggtgt
     4321 gctctcgggg gatttccgcc gaggagcggc cagcgactat accgttgcca gcttgaacgc
     4381 gccctctgac ccccaactcg acccctttga agccgcc
//
LOCUS       DAOTIP010000073         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23538_length_4404_cov_3.523569, whole genome shotgun sequence.
ACCESSION   DAOTIP010000073 DAOTIP010000000
VERSION     DAOTIP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_4404_cov_3.523569"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..601)
                     /gene="greA"
                     /locus_tag="P5138_02210"
     CDS             complement(125..601)
                     /gene="greA"
                     /locus_tag="P5138_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRV59419.1"
                     /translation="MTDSEESGESITADGLVKLKAELEELEGPAREKIAARLKAAREL
                     GDLKENAEYHIAKEDQAHLETKIQRLREREKNAVVVKLEESGDVFAFGRTAEVTDDSG
                     RDHSWTLVGSTEANLAEGRLSAESPVGQALRNRKVGEEVKIATPKGERTFTIRSVS"
     gene            complement(628..1386)
                     /locus_tag="P5138_02215"
     CDS             complement(628..1386)
                     /locus_tag="P5138_02215"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRV59420.1"
                     /translation="MSTGHDRFREQLPAYLLGALDPGEQGDLEHHLEGCPECRAELAW
                     LEPASTVLSADVEQIEPSPSLKSRVMAAVDADIADNPSPESGPAETSVPETSAAAGRD
                     DRRPSRPSGWLAGLFRPAVLGTVAAALFIGVAIGVVLDGGSNSTTGPARQVITGQSTI
                     GAEAVMVASNGTGTLKMSNLRRPGENQVYQAWIQRGQEIEPTDSLFVPNSDGSAVASI
                     PDISGVSAVMVSAEPKGGSQQPTTAPVITVSVPG"
     gene            complement(1383..1970)
                     /locus_tag="P5138_02220"
     CDS             complement(1383..1970)
                     /locus_tag="P5138_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV59421.1"
                     /translation="MGNQVPSQSPPDEDLLVRVGDRDKQAFELFYDRFERRAFSLAYR
                     IVGERSLAEDIVQDGFLSVWKNAGRYDPSRGSAGAWALGIIRNRAIDVLRSRASRTPD
                     LDHDDDLTLENRVSDLNTEGQAIQRETGREVRTRLAQLPENQSKVIGLAFFGGFSQTE
                     ISDLLELPLGTVKGRMRLGMEKLRGELGSYEGETT"
     gene            complement(2003..2647)
                     /locus_tag="P5138_02225"
     CDS             complement(2003..2647)
                     /locus_tag="P5138_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRV59422.1"
                     /translation="MTEPKTNTELTHPELAAIEVDGTERSDVLMKGALAAGAVFGLGA
                     IGPFVRKAMAANGSKGDIEILNYALTLEYLESAFYAEALKKAGLSGDLKQLVTTISTD
                     EDAHVKALTQTIKDLGGKPVKAPRVEFPYSDTAGFTALAETIENVGVSAYNGAAPMIS
                     SKDVLEAAGSIVQVEARHAAAIALQNGNQPAPDAFDKPMTEAQVLKAVQPFLVS"
     gene            complement(2674..3396)
                     /locus_tag="P5138_02230"
     CDS             complement(2674..3396)
                     /locus_tag="P5138_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRV59423.1"
                     /translation="MAATRQRSTEEKEEQEQQEGFGRSGVSRRQLVSMAGGSVALGAF
                     LAACGSDDSDSGSDSMSNGSGTTGTSDSSMSDDSTAQFGEGDIGIMNYALTLEYLETA
                     FYEDVAKSGLFKGSDLETIRKFGSQEAEHVAALTAAVKKAGGKPAAKPKTEFPLNNAK
                     QVLRLASTVENLGAAAYLGQAARIQSPDVLAAALSIHSVEGRHAAALNTLLGKTPVPD
                     GPFAAPAPAAMVLNAVQPFIVS"
     gene            3516..3680
                     /locus_tag="P5138_02235"
     CDS             3516..3680
                     /locus_tag="P5138_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRV59424.1"
                     /translation="MIPNIGPLEIALVLIIAIVVVGPGKLPELGRSAGKGLREFKGTL
                     AGGEDERENS"
     gene            3718..>4404
                     /locus_tag="P5138_02240"
     CDS             3718..>4404
                     /locus_tag="P5138_02240"
                     /inference="COORDINATES: protein motif:HMM:NF018332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59425.1"
                     /translation="MNRSTVLAMGAMALGVFVIANDITAMNVALPAIEKDFDTSVTTV
                     QWVVNAYALVFGVLIVTGGRLADLFGRREAFFVGASIFLIASLAAAVSQTEAWLIAAR
                     AVMGVGGALMWPAILGMTFALLPEEKAGLAGGLILGVAGIGNALGPLIGGALTEFASW
                     RWILFLNVPIAITAIIAVYFLIHQPRPETEDRQIDYGGIATVSLGLVLFMVALDQVVD
                     LGWGDPRIVVS"
BASE COUNT          680 a         1437 c         1394 g          893 t
ORIGIN      
        1 atccatacgg cgggccagtc gaccctcttg ccgtaatccc aggcccggcc catcagcccg
       61 acggccgctc cggcccggtt cgcctttgac ttcttgttcg ccatgaccga aggatacggt
      121 ccggtcagga gaccgaccgg atggtgaagg tccgctcgcc cttcggggtg gcgatcttca
      181 cttcctcccc gaccttgcgg ttgcggagcg cctggcccac cggtgactcg gcgctgagcc
      241 ggccttcggc caggttcgct tcggtggagc cgaccagggt ccagctgtgg tcccggcccg
      301 aatcgtcggt cacctcggcg gtgcggccga aggcgaagac gtcaccggac tcttccagct
      361 tgaccaccac tgcgttcttc tcgcgttccc ggagccgctg gatcttcgtt tccaggtggg
      421 cctgatcctc cttggcgatg tggtactcgg cgttctcctt caggtcgccg agttcgcggg
      481 cggccttgag cctggccgcg atcttctccc gcgcggggcc ctcgagctct tcgagctcgg
      541 ccttgagttt caccagtcca tcggcggtga tcgactcgcc gctttcctct gaatcagtca
      601 tggccccacc ctacccggat ggaaaactca gcccggaacc gaaacggtga tcaccggggc
      661 cgtggtcggt tgctggctgc caccctttgg ttcggcgctc accatgaccg cagaaacccc
      721 gctgatgtcg ggaatgctgg ccacagccga gccgtcgctg ttcgggacaa agagggaatc
      781 cgttggctcg atttcctggc cacgctggat ccaggcctgg tagacctgat tctcgcctgg
      841 gcgcctcaga ttgctcatct tcagggtccc ggtcccgttg gaggcgacca tgacggcttc
      901 ggccccgatc gtcgactggc cggtgatcac ctgacgagcc ggaccggtcg tggaattgct
      961 cccgccatca aggacgaccc cgatcgccac acctatgaaa agggcggcgg ccacggtgcc
     1021 cagtacggcc gggcgaaaca gaccggccag ccagccagag gggcgggagg ggcggcggtc
     1081 atcccgcccg gcggctgcgg aagtctcggg cacggatgtt tctgccgggc cgctctcggg
     1141 ggaggggttg tctgcgatgt cggcatccac tgccgccatc acgcgggact tcaacgaagg
     1201 agagggctcg atctgttcga cgtctgcgga aaggacagtc gatgccggct cgagccacgc
     1261 cagctccgcc ctgcattccg ggcagccctc gagatggtgc tccagatcac cctgctctcc
     1321 cgggtcgagt gcccccagca gataggccgg cagttgttcc ctgaatcggt cgtgcccggt
     1381 actcatgtcg tctctccttc gtaggagccc agctcacccc tgagcttctc cattccaagt
     1441 cgcattcttc ccttgacggt gcctaacgga agttcgagca ggtccgagat ctcggtctgg
     1501 ctgaaaccgc cgaagaaagc gagcccgatc accttcgact ggttctccgg aagttgggca
     1561 agcctggtgc gaacttcccg tccggtctcc cgctggatcg cctggccttc cgtgttcagg
     1621 tcggagaccc ggttttcgag ggtcaggtcg tcatcgtggt cgagatccgg agtcctgctc
     1681 gccctgctcc ggaggacgtc gatagcccgg tttcgaatga ttccgagcgc ccaggccccg
     1741 gcgctgcccc tcgacgggtc ataccggccg gcgttcttcc agaccgaaag gaaaccgtcc
     1801 tgcactatgt cctcggccaa cgacctttca cccactatcc gataggcgag cgagaaggcg
     1861 cggcgttcga accggtcgta gaagagctcg aacgcctgtt tgtcgcggtc gccgacccgg
     1921 acgaggagat cctcgtccgg gggcgactgc gacgggacct ggttgcccat ctcattcccc
     1981 ggtcctgttt ccccgatccc ggtcaggaga ccaggaaggg ctggaccgct ttcagcacct
     2041 gggcttcggt catcggcttg tcgaaagcat ccggtgcggg ctgattgccg ttctgcaggg
     2101 caatcgcggc cgcgtgcctg gcctcgacct ggacgatgct tccggccgcc tcgaggacgt
     2161 ccttcgagct gatcatcggg gccgcaccgt tgtaggcact gactccgacg ttctcgatcg
     2221 tttcggccag ggcggtgaac ccggcggtgt ccgaatacgg gaactcgacc ctgggggcct
     2281 tgaccggctt tccacccaga tccttgattg tctgggtgag cgccttcacg tgggcatcct
     2341 catctgtcga gatggtagtg accagctgct tcaggtctcc cgacagtccc gccttcttca
     2401 gggcttcggc gtagaacgcc gactcgagat actcgagcgt gagtgcgtag ttgaggatct
     2461 cgatgtcgcc ctttgaaccg ttcgcggcca tcgctttccg gacgaagggg ccgatggccc
     2521 cgagaccgaa gacggcaccc gcggcgagag cgcccttcat caggacgtcg cttcgctcgg
     2581 ttccatctac ttcgatcgct gccagttcgg ggtgggtcaa ttcagtgttt gtctttggtt
     2641 cagtcatctg tctgtctcct ttcctgggcc ggttcagctg acgatgaagg gctggacggc
     2701 gttgaggacc atcgctgcgg gggccggcgc ggcaaagggt ccgtcgggta ccggcgtctt
     2761 ccccagcagc gtgttgaggg ccgccgcgtg acggccttcg acgctgtgga tcgaaagggc
     2821 ggccgcgagc acatccgggc tctggatccg ggcggcctgg ccgagatatg cggctgcgcc
     2881 gaggttctcg accgtggacg caaggcgcag gacctgcttc gcgttgttca gcgggaactc
     2941 ggtcttcggc ttcgcggcgg gcttgccgcc ggccttcttc acggcggccg tcagggccgc
     3001 gacgtgctcg gcttcctggc ttccgaactt gcggatcgtt tcgaggtccg atcccttgaa
     3061 gagaccgctc tttgccacgt cctcgtagaa cgccgtctcc aggtattcga gggtcagcgc
     3121 gtagttcatg attccgatgt cgccctcgcc gaactgggcg gtgctgtcat cactcatcga
     3181 cgagtcgctg gttccggtag tgccggagcc gttcgacatc gagtcggatc cggaatccga
     3241 atcatcactg ccgcatgcgg caaggaatgc accgagggcg accgacccgc cggccatcga
     3301 caccaactgg cgccgggaca cgccgctcct gccgaaacct tcctgctgct cctgctcttc
     3361 cttctcttcc gtacttcgct gtctggtggc agccatggcg cctcctctgt tcgtttacgc
     3421 cgttgaaagc acttacggag cagaagcggg atcggatcaa tcgaatgtct tcgaagtgac
     3481 tgatccggaa ggccggccgg accgtagctc ttcacatgat tcccaacatc ggaccactag
     3541 aaattgccct cgtactgatc atcgccatcg tcgtcgtcgg gccagggaag ctgcccgaac
     3601 tgggacgctc ggcgggcaag gggctgaggg aattcaaagg caccctcgcc ggtggggaag
     3661 acgaacgcga gaattcatag gtcgcgatat gtcacttcca gcccataggc tgggagtgtg
     3721 aaccgttcaa cagttctcgc catgggggcg atggcgctcg gtgtcttcgt catcgccaat
     3781 gacatcaccg caatgaacgt cgcccttccg gcgatcgaaa aggacttcga caccagcgtc
     3841 accacggtcc aatgggtggt caacgcctat gccctggtct tcggggtgct gatcgtcacc
     3901 ggcggacggc tggccgatct cttcggccgc cgcgaggcgt tcttcgtcgg ggcgagcatc
     3961 ttcctcatcg cctcgctggc cgcggcggtc tcccagaccg aggcctggct gatcgcggcc
     4021 agggcggtga tgggagtcgg gggagcgctt atgtggccgg cgatcctcgg catgaccttc
     4081 gccctgttgc cggaggagaa ggccggattg gccggcggcc tgatcctcgg cgttgccgga
     4141 ataggcaatg cgctcggacc gctgatcggt ggcgccctga ccgaattcgc gagctggcgc
     4201 tggatcctct tcctcaacgt gccgatcgcg atcacggcga tcatcgccgt ttacttcctg
     4261 atccaccagc cacgccccga gaccgaggac cgccagatcg actacggggg gatcgcgacc
     4321 gtctctctcg gccttgtgct cttcatggtc gcgctcgacc aggtagtgga tctcggatgg
     4381 ggcgatccgc ggatcgttgt ctcg
//
LOCUS       DAOTIP010000074         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23539_length_4404_cov_3.094045, whole genome shotgun sequence.
ACCESSION   DAOTIP010000074 DAOTIP010000000
VERSION     DAOTIP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23539_length_4404_cov_3.094045"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="P5138_02245"
     CDS             complement(<1..707)
                     /locus_tag="P5138_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRV59426.1"
                     /translation="MTSNKLPQSVRIREVGPRDGFQNEPEVIATSDKIRLINLLAQTG
                     LRRIEVTSFVRPDVIPQLSDATEVLAGIDRPEGVSYSVLIPNRRGLENALAQRDLFDE
                     TNFFLSASETHNMKNVNRTVEESIAGLEETIPVAVEAGLRCEGVISTSFGCPYEGDVP
                     VENVLALALRLARAGCVEVGFGDTTGMANPVQVRRFFDEARERFASEGLGEVELTAHF
                     HDTRGQGLANVLAALEEG"
     gene            complement(726..1097)
                     /locus_tag="P5138_02250"
     CDS             complement(726..1097)
                     /locus_tag="P5138_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59427.1"
                     /translation="MSIAEKVGTEREENFTAEGMLLTDVGGLLETGVLSTPGMIGMME
                     RNCALLVQEFLPEGTATVGFAVNIKHVGGAGGGAECTAWARLDEVIEDRKFRFAVEVR
                     EGERVLGVGTHERRAITFNVA"
     gene            1155..1850
                     /gene="npdG"
                     /locus_tag="P5138_02255"
     CDS             1155..1850
                     /gene="npdG"
                     /locus_tag="P5138_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HRV59428.1"
                     /translation="MSENSDSPVIPVIGGTGALGSGLAKRWAAGGARVIIGSRDAGRA
                     GEAAAKIAGESGGSAEGMANEDAAKAADIVFLTIPFRNQSEYLTNLKGALSPGQILVD
                     CSVPLAAAISGKATRTIGIWQGSAAEQAQEMVPEGVTVVSALHTVSAPGLADLSHAMD
                     EDVPVCGDRKADKARVAELIARIDGLRPINAGALEISRIVEQLTALMISVNIRYKTHA
                     GLKFTNLPGGDAF"
     gene            1850..2776
                     /gene="cofD"
                     /locus_tag="P5138_02260"
     CDS             1850..2776
                     /gene="cofD"
                     /locus_tag="P5138_02260"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HRV59429.1"
                     /translation="MKVTLLAGGTGGAKLAAGMQSVVGSDLTVIANTGDDVETLGVYV
                     SPDPDLVSYWLAGQVDEVRGWGIKDDGFDVFQRMARFGAPDWFSLSNLDLAACLYRRD
                     FMDSGGRLTDAQAQIGRGLGIRATVLPMSDQPVRTMIKSGGEWRGLQEYLILEGGQAE
                     VEGVELRGIDAAETTPEVLDAIAGADLIVIGPSNPVISIGPILAVPGIREAIISAGAP
                     VIAVSPYVAGKVIKGPTDLFMKAIGRPTTASGVVSLYQGVIDAMINDSDDPDPPPTEV
                     RSFSTATLMHSAESRGTVARAVIKVGETLAAS"
     gene            2773..3426
                     /gene="cofC"
                     /locus_tag="P5138_02265"
     CDS             2773..3426
                     /gene="cofC"
                     /locus_tag="P5138_02265"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HRV59430.1"
                     /translation="MNTVAVVTAKRFTAAKQRMTGAVPVEKRLALVEAMLGDVLEAIT
                     EARQVTFTVVVTGEAAAAGLATGCGAEVIHDPDDAGHIGAAMLGVDRARELGADAVML
                     LPGDCPLLKAREIDHLVTGLPDPFVTVVPDRHGTGTNALVMRPPGAIEPAFGEGSCER
                     HLDLARQAGVPHATEQVDGLALDLDTPADIVALTTKIEMARDGGGNPAPRTAAVLGI"
     gene            3434..4186
                     /gene="cofE"
                     /locus_tag="P5138_02270"
     CDS             3434..4186
                     /gene="cofE"
                     /locus_tag="P5138_02270"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRV59431.1"
                     /translation="MGGAMIIPVEGLPEVTETFNLGKAIAEQVQLTDGDIVAVSQKVV
                     SKSEGRVVRLDLTDPGPEAISLAARTGKDPRIVQHILAESRNLVRVDEERGILITETH
                     HGFICANAGIDQSNTAGPEEAILLPLDPDGSARRIRVELETASGVRAAVVVTDSFGRA
                     WRHGQVEVAIGCAGIDPLDDWRGQSDQRGRELTATVIATADQVAAASDLVRTKTDGVP
                     AIVVRGLDPYVTGDDGPGCASQLREPDDDLFR"
BASE COUNT          781 a         1437 c         1492 g          694 t
ORIGIN      
        1 ccctcctcga gggcagccag gacattggcc aggccctggc cacgggtgtc gtggaaatgc
       61 gcggtcagct cgacctcccc cagaccttcg gacgcgaacc gctcacgcgc ctcgtcaaag
      121 aagcgccgga cctggaccgg gtttgccata ccggtggtgt cgccgaaacc gacctcgacg
      181 cagccggccc gggccagccg caaggcgagc gccaggacgt tctcgaccgg gacgtcaccc
      241 tcgtaggggc agccgaaaga ggtcgagatc actccctcac agcgcagtcc ggcttcgact
      301 gcgaccggaa tcgtctcttc gaggccggcg atcgactcct cgaccgtccg gttgacgttc
      361 ttcatgttgt gggtctcgga agcggacagg aagaagttgg tttcgtcaaa caggtcgcgc
      421 tgcgcgagag cgttctcgag accgcgtcgg ttcgggatca gcaccgaata cgaaacgcct
      481 tccggccggt cgatcccggc gaggacctcg gtcgcatcgg aaagctgcgg gatcacgtcg
      541 ggccggacga acgaggtgac ctcgatccgc cggagcccgg tctgcgccag caggttgatc
      601 aagcggatct tgtccgaagt ggcgatcact tcgggctcgt tctggaaacc gtcccgggga
      661 ccgacctcgc gaatccggac tgactgggga agtttgttgc tggtcacggt cgaaatgcct
      721 cccgctcagg cgacgttgaa ggtgattgcc cgacgctcgt gggtgccgac tccgagcact
      781 ctttcccctt cgcggacctc taccgcgaac cggaacttgc ggtcttcgat cacctcgtca
      841 agacgggccc aggccgtgca ttccgcacct cctccggccc cgcccacatg cttgatgttg
      901 accgcgaagc cgaccgtggc cgtgccttcc ggcaggaact cctgaaccag cagcgcgcag
      961 ttcctttcca tcatcccgat catgcccggg gttgaaagga cgccggtttc gagcagacca
     1021 ccgacgtcgg tgagcagcat cccctcggcg gtgaaattct cttcgcgctc ggttccaacc
     1081 ttttcggcaa tcgacattgg cagagcctat atcgggtgat ggccggaccc acttcctgct
     1141 tagactgcgg cttcgtgagc gagaacagcg attcaccagt catccctgta atcggcggca
     1201 cgggagctct cggctccgga ctcgccaaac gctgggcggc cggcggcgcc agggtgatca
     1261 tcggctcgcg cgacgcgggt cgggccggtg aggccgccgc gaagatcgcc ggggaatccg
     1321 gcgggagcgc agagggaatg gccaacgagg acgcggccaa agccgccgac atcgtgttcc
     1381 tgacgatccc gttccgcaac cagtcggaat atctgaccaa cctcaaggga gcgctctccc
     1441 ccggccagat cctggtcgac tgctctgtcc ccctggcggc cgcgatcagc ggcaaggcga
     1501 cccggacgat cggcatctgg cagggatcgg cggccgaaca ggcacaggag atggtgccgg
     1561 agggagtgac ggtggtctcc gccctgcaca cggtcagcgc ccccggcctg gccgacctgt
     1621 cccatgcgat ggacgaggac gtgccggtct gcggcgacag gaaggcggac aaggccaggg
     1681 tggcggagct gatcgcgcgc atcgacggcc ttcgcccgat caacgccggg gcacttgaga
     1741 tctcccggat cgtcgagcag ctgaccgccc tgatgatctc ggtcaacatc cgctacaaga
     1801 cccacgccgg cctgaagttc accaacctgc cggggggcga cgccttctga tgaaggtcac
     1861 cctcctcgca ggcggaaccg gcggcgccaa gcttgccgcc gggatgcagt cggtggtcgg
     1921 ctcggacctc accgtgatcg ccaataccgg ggatgacgtc gagaccctgg gggtttatgt
     1981 ctcgcccgat cccgacctgg tcagctactg gctggcaggc caggtcgacg aggtgcgcgg
     2041 atggggcatc aaggacgacg gcttcgacgt ctttcagcgc atggcccggt tcggcgcgcc
     2101 cgactggttc tcgctctcca atctcgacct cgcagcctgc ctctaccgcc gggacttcat
     2161 ggattccggc gggaggctga ccgacgccca ggcccagatt ggcaggggcc tcgggataag
     2221 ggcgacggtg ctgccgatgt ccgaccagcc ggtccggaca atgatcaaga gtggcggcga
     2281 gtggcgcggt ctccaggagt acctgattct cgagggcggc caggccgagg tcgagggagt
     2341 cgaactccgc ggcatcgacg cagccgaaac cacacccgaa gtacttgacg cgatcgccgg
     2401 ggccgatctg atcgtgatcg ggccctccaa cccggtgatc agcatcggcc cgatcctcgc
     2461 cgtgccggga atccgagagg cgatcatctc ggccggggct ccggtcatcg cggtcagccc
     2521 ctatgtcgcg ggcaaggtga tcaagggacc gaccgacctc ttcatgaagg cgatcggccg
     2581 cccgaccacg gcgtcaggtg tcgtcagtct ctatcagggg gtgatcgacg cgatgatcaa
     2641 tgactccgac gatcccgacc cgccgccgac cgaggtgagg tcgttctcga cggcgaccct
     2701 gatgcacagc gccgagagcc gcggaaccgt ggcgagggcc gtgatcaagg tcggcgagac
     2761 cctggcagca tcgtgaacac ggtcgccgtc gtcaccgcga agcgcttcac cgcggccaag
     2821 cagcggatga cgggagcggt tccggtggag aagcgactgg cgctggtaga ggcgatgctc
     2881 ggggacgttc tggaggcgat caccgaagcc cgacaggtga cctttacggt ggtggtgacc
     2941 ggagaagcgg ccgccgcggg gctggctacg ggttgcggcg ccgaggtgat ccacgatccc
     3001 gacgacgccg gacacatcgg ggcggcgatg ctcggggtcg accgtgcccg ggagttgggt
     3061 gccgatgccg tcatgctgct gcccggagac tgcccgctgc tgaaggcaag ggagatcgac
     3121 cacctcgtga ccggcctgcc cgatcccttc gtcaccgtgg ttccggaccg gcacggaacc
     3181 ggaaccaatg ctctcgtgat gcgacccccg ggcgcaatag agccggcttt tggcgaggga
     3241 agctgcgagc gtcacctcga cctggccaga caggccggtg tcccccacgc gaccgaacag
     3301 gttgacggac tggccctgga cctcgacact ccagccgaca tcgttgccct gaccacaaag
     3361 atcgagatgg cccgtgacgg cgggggcaac cccgctccgc ggacggctgc cgtgctgggg
     3421 atctgaccgg gccatgggcg gagcgatgat cattccggtc gaaggactcc cggaagtgac
     3481 cgagaccttc aacctgggca aggcgatcgc cgagcaggtc cagttgaccg acggcgacat
     3541 cgtcgcggtc tcgcagaagg ttgtctcgaa gtccgaggga agggtcgtcc ggctcgatct
     3601 gaccgacccg ggtcccgagg ccatctcgct ggccgcacgc accggcaagg acccgcggat
     3661 cgtccagcac attctggccg agagccggaa cctggtgcgg gtggacgagg aacggggcat
     3721 tctcataacg gagacgcatc acggcttcat ttgcgccaac gccggcattg accagtcgaa
     3781 caccgccggt cccgaggagg cgatcctgct tccgctggac cccgacggat cggccagacg
     3841 gatcagggtg gaactggaaa cggcctcggg agtacgggca gccgtggtcg tgaccgacag
     3901 cttcggccgg gcctggcgcc acggacaggt cgaggtggcg atcggctgcg cggggattga
     3961 cccgctcgac gactggcggg gccaaagcga ccagcgcgga agggaactca cggcgaccgt
     4021 gatcgccacc gccgatcagg ttgccgccgc atccgacctg gtcaggacca agacggacgg
     4081 cgttccggcg atcgtggtcc ggggtctgga cccgtatgtg accggtgacg acggaccggg
     4141 ctgcgcctca cagctgcgag aaccggacga cgacctcttc cgctgagtcg gaccggattc
     4201 ggccggccac cgggcggata ctctccagtc atgggaagac tggtccgggc cagcatggtc
     4261 tccattgacg gtttctacga agacgccgac ggcacgatcg agtggggaat gccggaaccc
     4321 gaactgcatt cgttcgcgaa cgagaaagtc ggtgacctgg cggcgatcgt gatggggcgt
     4381 cgcctctacg aggtgatggt cccc
//
LOCUS       DAOTIP010000075         4400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23577_length_4400_cov_2.699425, whole genome shotgun sequence.
ACCESSION   DAOTIP010000075 DAOTIP010000000
VERSION     DAOTIP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23577_length_4400_cov_2.699425"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..438)
                     /locus_tag="P5138_02275"
     CDS             complement(7..438)
                     /locus_tag="P5138_02275"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRV59432.1"
                     /translation="MPTVSRKRTVPATPESVYDLISDPARLAEWWPRVVRVEDVAGRA
                     GTERTRWTSVLGADSGRMLRLDFRCTGATRPERYEWEHELEGTPYESHLARQAVEVRI
                     AESDEGSEVNLTTVNSLRGTAKIAGFSMRKGQRGCRGRADQ"
     gene            complement(445..1185)
                     /locus_tag="P5138_02280"
     CDS             complement(445..1185)
                     /locus_tag="P5138_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRV59433.1"
                     /translation="MTLSDQNLADGAQMAPRRPRKALTIGAVAKLLSKEFDDVSISKI
                     RYLEDQKLVTPRRTSGGYRLYSQADVDRLRTVLRLQRDEFLPLRVIRQELAAGPSVSG
                     LSGNSSNTARRAKLIGTTSSRFTVEEVMEETGARADFIRELAEFGLVTPKTDNGKAVY
                     DETDMEIVRAANELARSGGGARYLKLFKTSADREVNLIETLLAPKLASRNPQRRKEAV
                     ESLERMATVLSELKHALLVRDLRRLADS"
     gene            complement(1187..1657)
                     /locus_tag="P5138_02285"
     CDS             complement(1187..1657)
                     /locus_tag="P5138_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRV59434.1"
                     /translation="MATHCPECGLVNPEAANYCQKCGTFIGRREGGDDQGTSTYQVGE
                     TGEIEAVDVDAEVERTGAALVIRSGGGRAGETFPMEEDRVAIGRSPDAGVFLDDVTVS
                     RNHALLVSRGDGTYIDDLGSLNGTYVNRKRIESQRLEDGDEIQVGKYKLTYLER"
     gene            complement(1700..2371)
                     /locus_tag="P5138_02290"
     CDS             complement(1700..2371)
                     /locus_tag="P5138_02290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRV59435.1"
                     /translation="MGDAAPFGKGRIRKLFGLDRTGAEPAPTRAGQPLRPFTIPNFIG
                     YLRLIGIPLFLIVAFSSDDGRDWTAALLFFLIAGGDYVDGFLARVTGQYSRLGALMDP
                     VIDRLTIFSGLVVCWHFELLPRWMLALLLAREIVTLLLARWGLSHGVDIEVNWFGRVA
                     VFPVMISLLLALLVDTWIATVLLAIGIVLAMIATYLYARVGRDQINARQAAGEDPAAG
                     ESAQP"
     gene            complement(2371..4065)
                     /locus_tag="P5138_02295"
     CDS             complement(2371..4065)
                     /locus_tag="P5138_02295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRV59436.1"
                     /translation="MTRISRTLLPTLKDPPADAEALSHKLLVRAGMVRQMGAGLWTYL
                     PAGWRAHRKVEQIIREEMDRIGGQEISMPVLQPADPWEKTGRYGIDELFKLEDRKGSP
                     MVLAMTHEECVTGHMANEIRSYRDLPQLVYQLQTKERDEPRPRAGILRTREFVMKDAY
                     SFDRDEAGLQETYNLCIEAYDRIYDRSGIRWYRVESDVGMMGGSGAHEYMAPCGAGED
                     TIALAPGYAANVEVASANPRPIEAGEQLGGPAEVETPGLKTVEEVSSALGVDPGALIK
                     SVPVITDEDEFILVLVRGDHQLNPIKLANALGIQSRPAREDEIEQKLGPPGYIGPVGA
                     SVRILKDRAIGGTGLVAGANRPDLHLKGVEPGRDFAFEEADVRSVVEGDTTDDGHPIT
                     LEPAIEVGNIFQLGTRYSVPLGATFLDENGKEQPIVMGSYGIGPARIVAAAAEQFADD
                     KGLSWPPAITPWQIHLVSLSKPDEPERAEADSLYEALLEGGFEVLYDDRDAGPGQKLT
                     DAELIGCPLRVVVGRRGLADGIYEGSERRSGQEHRIPVDGGAEEIARIYAALSGSD"
     gene            complement(4101..>4400)
                     /locus_tag="P5138_02300"
     CDS             complement(4101..>4400)
                     /locus_tag="P5138_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59437.1"
                     /translation="AWVTASGAAGSRLVFTYIDVALLNEGGEDEPPWSAQVAKVGEPF
                     RYGLDPAQMDEFLGARRLKPIWDLSTAEALEKHPVPGGRNAPDFYRAAEAEVLPG"
BASE COUNT          694 a         1474 c         1393 g          839 t
ORIGIN      
        1 tcaccatcac tgatcagctc gacctcggca cccacgctga cccttcctca tcgagaagcc
       61 ggcgatcttg gcggtgccac gcagcgagtt gaccgtggtc aggttcactt cggagccctc
      121 gtcgctttcc gcgatcctga cttccaccgc ttgcctggcc aggtgggatt catagggggt
      181 gccctccagt tcgtgctccc actcgtaacg ctcgggccgg gtcgccccgg tgcagcggaa
      241 atcgagtctc agcatccgcc cggaatcggc cccgagcacg ctggtccagc gggtccgttc
      301 ggtgcctgcc ctgccagcca cgtcttcgac cctgaccacc ctgggccacc attccgccag
      361 ccgggccggg tcacttatga ggtcgtagac actttccggc gtggccggta ctgtcctctt
      421 gcgactgacg gtcggcaccg cggcctagct gtcggcgagg cggcgcaggt ccctgaccag
      481 cagggcatgc ttgagctcgc tcagcacggt cgccatccgc tcgaggctct caaccgcctc
      541 cttgcgacgc tgcggattgc gcgaggcgag tttgggggcg agcagggtct cgatcaggtt
      601 cacttcccga tcggccgagg tcttgaacag cttcaggtac ctggctccgc ccccggaacg
      661 ggccagttcg ttggcggccc gcacgatttc catatcggtc tcgtcataga ccgccttgcc
      721 gttgtcagtc ttgggcgtta ccaggccgaa ctcggcgagt tcgcggatga aatcggctcg
      781 ggctccagtc tcctccatga cttcctcgac cgtaaagcgg gaactggtgg ttccgatcag
      841 tttcgcgcga cgggcagtgt ttgacgagtt tccggaaagg ccgctgaccg acggtccggc
      901 cgccagttcc tgccggatca ccctgagcgg cagaaactcg tcccgctgaa gcctcaggac
      961 cgtacgcagc cggtcgacgt cggcctggct gtagaggcga tagccgccgg aggttcgcct
     1021 tggagtgacc agtttctggt cttccagata gcggatcttc gagatcgaga cgtcgtcgaa
     1081 ttccttgctg agcagcttgg ccactgcccc gattgtcagt gccttgcggg gccggcgagg
     1141 cgccatctgg gcgccgtcgg caaggttctg gtcgctgagc gtcatctcat ctctccagat
     1201 acgtcagttt gtatttcccg acctggatct cgtccccgtc ctcaaggcgc tgggactcga
     1261 tccgtttgcg gttcacatag gtgccgttca gcgagccgag gtcatcgatg taggtgccgt
     1321 cgccccggga aacgagaagc gcgtggttgc gcgaaaccgt tacgtcatcg aggaataccc
     1381 cggcatcggg gctgcgcccg atggcaacgc gatcctcctc catcggaaag gtctctccgg
     1441 cccggccccc gccgcttcga atcaccaggg cggctccggt gcgttcgacc tcggcatcga
     1501 cgtctacagc ttcgatctcc cccgtctctc ccacctggta ggtggaagtc ccctggtcgt
     1561 caccgccttc acgtcggccg atgaaggtcc cgcacttctg gcagtaattg gcggcctcgg
     1621 ggttaaccaa tccgcattcg gggcagtgag tcgccatgcc ggcaattata gggcccgacc
     1681 ctaaccttca actcaggctt tacggttggg ccgattctcc cgccgcgggg tcctcgccgg
     1741 cagcctgcct cgcgtttatc tggtccctgc cgaccctcgc gtagaggtag gtcgcgatca
     1801 tcgcgaggac gatgccgatc gccagcagca ccgttgcgat ccaggtgtcg acgagcagcg
     1861 cgagcaggag ggaaatcatc accgggaaca ccgctacccg tccgaaccag ttcacctcga
     1921 tgtccacccc gtgggaaagt ccccatctgg ccagcagaag agtcacgatc tcgcgggcca
     1981 ggagcagggc gagcatccag cgcggcagca gttcgaaatg ccagcagacg accagcccgg
     2041 aaaagatcgt gagccggtcg atgaccgggt ccatcagggc accgaggcgg ctgtactggc
     2101 cggtgaccct ggccagaaaa ccatcaacgt agtctccgcc ggcgatcagg aagaagagca
     2161 gcgccgccgt ccagtcgcgg ccgtcgtcgg agctgaaggc cacgatcaga aaaagcggga
     2221 tcccgatcag ccgcaggtaa ccgatgaagt tgggaatcgt gaagggccgg aggggctgcc
     2281 ccgccctggt cggcgccggc tcggcaccgg tccggtcgag cccgaagagc ttccggatcc
     2341 tgcccttgcc gaagggtgct gcgtcgccca tcagtccgat ccggacagcg cggcgtagat
     2401 cctggcgatc tcctccgccc cgccatccac cggaatccgg tgttcctgtc cgcttcgacg
     2461 ctcgcttccc tcgtagatcc cgtcggcgag tccccggcgg cccaccacga cccgcagcgg
     2521 gcatccgatc agttccgcgt cggtcagctt ctggcccgga ccggcgtcac ggtcgtcata
     2581 gagcacctcg aaaccgcctt ccagcagggc ctcgtagagg ctgtccgctt cggccctttc
     2641 cggttcgtcc ggcttggaca gggagaccag gtgaatctgc cagggtgtga tggccggcgg
     2701 ccaggagagg cccttgtcgt cagcgaactg ctcggctgct gcggcaacga tccgtgccgg
     2761 gccgatgccg taactgccca tgacgatcgg ctgctccttg ccgttttcat ccaggaaggt
     2821 ggctccgagc ggcacgctgt accgggtgcc gagctggaag atgttgccga cctcgattgc
     2881 cggctcgagg gtgatcggat gaccgtcgtc ggtcgtgtct ccctcgacga ccgagcgcac
     2941 gtcggcttcc tcgaaggcga agtcacggcc gggctccact cccttgaggt gaaggtcggg
     3001 ccggttggcc ccggcgacca gaccggttcc gccgattgcg cggtccttga ggattctcac
     3061 cgaagcgccg accggaccga tgtaaccggg cggccccagc ttctgctcga tctcgtcttc
     3121 ccgggcggga cgggactgga tcccgagggc gttggccagc ttgatcgggt tgagctgatg
     3181 gtcgccccgg accagcacca gaatgaactc gtcctcgtcg gtgatcacgg gaaccgactt
     3241 gatcagggca cccggatcga caccgagtgc ggaggaaacc tcttcgaccg ttttcaggcc
     3301 gggcgtttca acctcggccg gcccgcccag ctgttcgccc gcctcgatcg gacgcgggtt
     3361 cgcggaggcg acttcaacgt tcgcggcgta gcccggtgcg agagcaatgg tgtcctcgcc
     3421 ggcgccgcag ggggccatgt actcgtgggc accggagccg cccatcatgc cgacgtcgct
     3481 ttcgacccgg taccaccgga tgccgctgcg atcgtagatc cggtcgtaag cctcgatgca
     3541 gaggttgtac gtctcctgaa gaccggcttc gtcacggtcg aagctgtagg cgtccttcat
     3601 cacgaattcc cgggtcctca ggatcccggc gcggggtcgc ggctcgtccc gctccttggt
     3661 ctggagctgg tagacgagct gcggcagatc ccggtaggac cgaatctcgt tggccatgtg
     3721 accggtcacg cattcctcgt gggtcattgc cagaaccatc ggcgagccct tgcgatcttc
     3781 gagcttgaac agttcgtcga ttccgtagcg tccggtcttc tcccaggggt cggccggctg
     3841 gagtaccggc atcgagatct cctggccgcc gatccggtcc atctcttcgc ggatgatctg
     3901 ctccaccttg cggtgggcgc gccagcctgc gggcagatag gtccagagcc ctgcccccat
     3961 ctgacgcacc attccggccc ggaccagcag tttgtggctc agcgcctccg catcggcggg
     4021 cgggtctttc agggtaggca gaagggtgcg cgaaatcctc gtcatcggag tcgcagccta
     4081 taggccctcg gctggcggaa tcagccaggc aggacttcgg cttccgccgc ccggtagaag
     4141 tcgggcgcgt tgcgtccgcc ggggaccgga tgcttttcga gtgcctcagc agtcgagagg
     4201 tcccagatcg gtttgagtct ccgtgctccg aggaattcgt ccatctgtgc cgggtcgagt
     4261 ccgtagcgga acggttctcc gactttcgcc acctgcgcac tccagggcgg ctcgtcttcc
     4321 ccgccttcgt tcagcagggc gacgtctata taggtgaaga ccagccggct tccggccgcg
     4381 cccgatgcag tgacccaggc
//
LOCUS       DAOTIP010000076         4391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_23632_length_4391_cov_3.609779, whole genome shotgun sequence.
ACCESSION   DAOTIP010000076 DAOTIP010000000
VERSION     DAOTIP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23632_length_4391_cov_3.609779"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..2184)
                     /locus_tag="P5138_02305"
     CDS             complement(163..2184)
                     /locus_tag="P5138_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59438.1"
                     /translation="MNPDQATDSPEPHRWYRIWGEIAGAWAIALAYPVYTKIASGPEA
                     LTALGLRRLDVLVLIFVVSLLGPLLITLAELGIRKLFGERVRRIVQGLVVGALLSLVI
                     WQWLLDHGSPGFLRNLLPLAFALLVAWLYVRTELIRNFALMLSFATVVVIGAFAIDYP
                     IKDELLPHKAAASTAKVDADTPVVMVIFDELPLAALEKPDGKIDPRFRNFAMLARKAN
                     WYSDAVSVADQTTFALPSILTGQDPNPTGGNHPPSPSLANYPNSICQIAENGGYEVHA
                     YEPVTDLCHRSWDMGTRITGTIRRAISADNPIYQDEIAPSELDRRAARAAGSFFRKPY
                     SEIDEGRREAFNEFIDGLPSDSRSLSVLHITLPHVMWIYSPDGSQYEDFRPAADTLLT
                     NPSTEGEVERNAQQMMLQLQFTDRELGKLIRKMKADGTWDESLFIVTADHGAAFHKDG
                     SRRILDSTNEGWIMPVPLFIKYPGQTKGKVVRGKANGLDIAPTVMSTLGLDPLPDMHG
                     RDLTGVSKLPRTKSIEMVGTIGGRTSIGLARVRKEQKQASSFMNRLLGRSFFAPGGYA
                     NLLGRRPSGMTEVAYTATDPTLYENVDRSSGFIPAYFQAGLSVPGNRYPGTMAIALNG
                     RIVATGRPWKYDGQWYSGVVLPAEKFRDGSNEIRVYRVNPEKAASPQTR"
     gene            complement(2215..3090)
                     /gene="sucD"
                     /locus_tag="P5138_02310"
     CDS             complement(2215..3090)
                     /gene="sucD"
                     /locus_tag="P5138_02310"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRV59439.1"
                     /translation="MSIIIDNETKLVVSGLTGREGSFHGLRNKAYGTQLVAGVTPGKG
                     GQDVEGTPIFNSIAESVEQAGANTSMIFVPAKFAADSINEAIDSGVDTVIAITEGIPV
                     MDMLSTYWKAREKGVRLIGPNCPGVLSPGKANVGIIPAQFFDQGRIGVVSKSGTLTYQ
                     IGNELKQAGEGNSTIVGIGGDPIIGSDFIDILTLFDSDPETDLIVMVGEIGGDAEERA
                     ADFIAAEISKPVVAYIAGFTAPPGKQMGHAGAIISGSSGTAKAKQEALEAKGVRVGES
                     PTEAAQIAVETLRSL"
     gene            complement(3102..4262)
                     /gene="sucC"
                     /locus_tag="P5138_02315"
     CDS             complement(3102..4262)
                     /gene="sucC"
                     /locus_tag="P5138_02315"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRV59440.1"
                     /translation="MDLLEYQGKELFARHGLPVPKGVHASTADAAVDASETLGYPVAV
                     KAQVLIGGRGKAGGIKIAKDSTEAHEYAGDIIGMDIIGPHGEGPFKVHELWVEAGSEI
                     ESEFYASVILDRSAKKLMVMLSRMGGMDVEAIAASDPEALVKQHVDPLAGLGKEEARE
                     IVDWAQIPDAVAWQVADVLVQLVEVAQAEDATLIEVNPLIVNADGEVILLDAKVTIDG
                     NSLFRHPELEELPESADQDAQEKMAKERGLTYVSLDGNIGILGNGAGLCMSTLDVVAQ
                     AGGRPANFLDAGGGSKAEAIIDALEVITSNPEVTAILFNIFGGITRCDEVARGIIEAT
                     NKIGLELPLVVRLDGTNSEEGLALLAEAGLDNLHHEATMLGAAKKVVELAGN"
BASE COUNT          702 a         1364 c         1462 g          863 t
ORIGIN      
        1 tgtcagagat ccactgtgcc tcgtcgatcg agaaccagcc gtcgcgcccg taatagagga
       61 tcagcggtgt tgcgattacc atcgcgacgg cgagtactgc ccaggccagc cgggagccgt
      121 tctcgccgcg tctcaggcgg tcaacctttt cggccgcgga gttcatcggg tttgaggtga
      181 ggccgctttc tccggattga ccctgtagac gcggatttcg ttgctgccat ccctgaactt
      241 ctccgccggc aggacgacgc cgctgtacca ctgcccgtcg tatttccacg gccttccggt
      301 cgcgacgatc ctgccgttca gggcgatcgc catggttccc ggataacggt tgcccgggac
      361 cgagagcccg gcctggaaat aggcggggat gaagccagag gaacggtcca cgttctcgta
      421 gagcgtcgga tccgttgccg tgtaggccac ctcggtcata ccggaaggcc tgcggccgag
      481 cagattggca tagccaccgg gggcaaaaaa ggatcgcccg agcaggcggt tcatgaagct
      541 gctggcctgt ttctgttcct tccgcacccg ggcgagcccg atgctggtcc tgcctccgat
      601 cgtgccgacc atctcgatgc tcttcgtccg gggcaatttg ctgacgccgg tcaggtcccg
      661 gccgtgcatg tcgggaaggg ggtcgagtcc gagggtggac atcacagtcg gagctatgtc
      721 cagaccgttg gccttccccc ggacaacctt gcccttcgtc tggcccgggt acttgatgaa
      781 gagcggcacc ggcatgatcc agccttcgtt cgtggaatcg aggatccgcc ggctgccatc
      841 cttgtggaag gctgcgccgt ggtccgcggt gacgatgaac agcgactcgt cccaggtccc
      901 gtcggccttc atcttgcgga tcagcttgcc cagctcccga tcggtgaact ggagctgaag
      961 catcatctgc tgggcgttcc tctcgacctc gccctcggtc gaggggttgg tcagcagggt
     1021 gtcggcggcc ggccggaagt cctcatactg actgccgtcc ggcgaataga tccacatcac
     1081 atggggcagg gtgatgtgga gcacgctgag cgaccgcgag tccgacggca gcccgtcgat
     1141 gaactcgttg aacgcttccc ggcggccctc gtcgatttcg ctgtagggct tgcggaagaa
     1201 cgagccggcg gcccgtgcgg cccggcggtc gagctcactc ggggcgattt cgtcctggta
     1261 gatcgggttg tcggcgctga tcgcccggcg gatcgtgccg gtgatccgtg tgcccatgtc
     1321 ccagcttcgg tggcagagat cggtcaccgg ctcgtaggcg tggacttcat agccgccgtt
     1381 ttcggcgatc tggcagatgc tgttcggata gttcgcgagg ctgggcgagg gcggatggtt
     1441 gccgccggtc gggttcgggt cctgcccggt caggattgaa ggcagggcga aggtggtctg
     1501 gtcggcgacg ctgaccgcgt ccgaatacca gttggccttt cgggcgagca tcgcgaagtt
     1561 cctgaaacgc ggatcgatct ttccgtcggg cttctcgaga gcagcgagcg gcagttcgtc
     1621 gaagatcacc atcacgaccg gggtgtccgc atcgactttc gcggtcgacg ccgccgcctt
     1681 gtgtggcagg agttcgtcct tgatcggata atcgatcgcg aaggcgccga tcaccacgac
     1741 ggtcgcgaag gagagcatca gggcgaagtt ccggatcagt tcggtcctga catagagcca
     1801 ggcgaccagc agggcaaacg cgagcggcag caggttgcga aggaaacccg gggacccgtg
     1861 atcgagcagc cactgccaga tgaccaatga aaggagcgct ccgacgacta ggccctggac
     1921 gatccggcgg acccgctccc cgaagagctt ccggatcccc agttcggcca gcgtgatcag
     1981 gagcggaccg agcagcgaga ccacgaagat caggaccagg acatcgagtc gcctcagccc
     2041 gagcgcggtc agggcttcgg ggccggaggc gatcttcgta tagaccggat aggcgagggc
     2101 gatcgcccag gctccggcta tctcccccca gatccggtac cagcggtgag gttcggggct
     2161 gtcagtcgcc tggtccgggt tcatgtcggt ccgccgccgg cgggcttgtc gtggctacag
     2221 ggaacgaagc gtttcgaccg cgatctgcgc ggcctcggtc gggctttcgc cgaccctcac
     2281 tcccttggct tccagcgcct cctgcttggc ctttgcggtc cctgacgatc cggaaatgat
     2341 cgcaccggcg tggcccatct gcttgcccgg aggagcggtg aacccggcga tgtaggcgac
     2401 gaccggcttg gagatctcgg ctgcgatgaa atcggcggcc cgttcctcgg cgtcgccgcc
     2461 gatctcaccg accatcacga tcaggtcggt ctcggggtcg ctgtcgaaga gggtgaggat
     2521 gtcgatgaaa tccgacccga tgatcgggtc gccgccgatg ccgacgatgg tcgagttgcc
     2581 ttcgccggcc tgcttcagct cgttgccgat ctggtaggtc agggtgccgg acttggacac
     2641 gacgccgatc cggccctggt cgaagaactg ggccgggatg atcccgacgt tggccttgcc
     2701 gggggagagt acgccggggc agttcggccc gatcaggcgg acgcccttct cgcgggcctt
     2761 ccagtaggtg gacagcatgt ccatcaccgg gattccctcg gtgatcgcga tgaccgtgtc
     2821 cacgccggag tcgatcgcct cgttgatcga atccgccgcg aacttcgccg gcacgaaaat
     2881 catcgaggtg ttggcgcccg cctgctcgac cgattcggcg atcgagttga agatcggggt
     2941 gccttcgacg tcctggccgc ccttgccggg ggtaaccccg gcgacaagct gtgtgccgta
     3001 cgccttgttg cggagtccgt ggaagcttcc ctcgcgcccg gtcaggccgg agacgaccag
     3061 cttcgtttcg ttgtcaatga tgatgctcat tgcttcagtc ctcagttccc ggccagttcg
     3121 acgaccttct tggcggcgcc gagcatggtt gcttcgtggt gcaggttgtc gagtccggcc
     3181 tcggccagca gggccaggcc ttcctccgag ttggtgccgt cgaggcggac caccaggggc
     3241 agttccagtc cgatcttgtt ggtggcctcg atgatgccgc gggcgacctc gtcacagcgg
     3301 gtgatcccgc cgaagatgtt gaagaggatc gcggtgacct cggggttcga ggtgatcacc
     3361 tcgagagcgt cgatgatcgc ctccgccttg gatcctcccc cggcgtcgag aaagttcgcc
     3421 ggccggccac cggcctgtgc gaccacgtca agggtgctca tgcagagccc ggcgccattg
     3481 ccgaggatgc cgatgtttcc gtccagggag acgtaggtca gtccacgttc cttggccatc
     3541 ttctcctgcg cgtcctgatc ggccgattcg ggcagctctt ccagttcggg atggcggaag
     3601 agcgagtttc cgtcgatcgt gaccttggcg tcgagcagga tcacctcgcc gtcggcgttg
     3661 acgatcagcg ggttgacctc gatcagggtc gcgtcctcgg cctgggcgac ctcgaccagc
     3721 tggaccagca cgtcggcgac ctgccaggcc accgcatcgg gaatctgtgc ccagtcgacg
     3781 atctcccggg cttcctcctt gccgagtccg gccagcggat cgacgtgctg cttgaccagt
     3841 gcttcgggat cagaggcggc gatcgcctcg acgtccatgc ctcccatgcg ggaaagcatg
     3901 accatcagct tcttggccga acggtcaagg atcaccgagg cgtagaactc cgattcgatc
     3961 tcggagccgg cttcgaccca gagttcgtga accttgaagg gaccttcgcc gtgggggccg
     4021 atgatgtcca tgccgatgat gtcgccggca tattcgtggg cttcggtgct gtcctttgcg
     4081 atcttgatgc cgccggcctt gccgcggccg ccgatcagta cctgcgcctt gacggcgacc
     4141 gggtagccga gagtttcaga tgcgtctact gcggcgtcag cggtggaagc gtgaacgccc
     4201 ttggggacgg gaagcccgtg gcgggcaaac agttcttttc cctgatattc gaggagatcc
     4261 atgacctttg aggagttggg agttggaggg ctccgcaggc cgtggtcggc cacgtcgaag
     4321 ccgacgatgg actctattcg acataatgcc cttcccgcat gccgctgcct tccctaaaga
     4381 acctcgagtg g
//
LOCUS       DAOTIP010000077         4313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_24225_length_4313_cov_3.113199, whole genome shotgun sequence.
ACCESSION   DAOTIP010000077 DAOTIP010000000
VERSION     DAOTIP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24225_length_4313_cov_3.113199"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..2241)
                     /locus_tag="P5138_02320"
     CDS             complement(184..2241)
                     /locus_tag="P5138_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRV59441.1"
                     /translation="MGALATFAFACLLMASPSLASAGWTSPATVSGPGTSVSQTTVAG
                     GSDGTSWVVWKRDVGGFNLIQGTRVQIDGTQGPIVTLSPVGQNANNPVIASRADGSAV
                     VGWTNESGTSDFIQSVSIAADGTVGAVTDRSVNGPAGQDASDLAIAVGADGTAGLAWL
                     RFDGTEPVVQAVRVASDGSSGTVHNLSAASAAPGPPDVGAAPPTTAGDPYFYRVMWPQ
                     GSAATSQVANADVMANDTVTTTVLLYTNASGADCADPFDIQIAYGSDGAMNAFWVCYK
                     KTIDFNTFNTFYNWSVQWLRTAKGVPLSTGGAASATNASPTIYLSPYPISGVVAAESF
                     TGQPAIAWIHDLDGGGQRVETWRIMINVLNGVPLPVGQGWANTTTEATAVGSPAIAVN
                     SNNTGIAGGVELGALPGQNTTNFTRFSNSSYDPVTPSGGFTYSEDPGYVLTDGGKTLA
                     AFTGITGPVGSAMVMTFEDPGISVDPDSFNFGHADIGTKRSVSISIRSTGQTTNTVTG
                     ITLGGANADRYAMSGADDCIREMTPNTNCRFTVTFTPGSTASQTAQISVTSDGGNEVA
                     NLTGTGLNRTRNSITAKPGHAARRKGKVVRIRVKASNLGGVSSNNTRICVNLRKRALK
                     LAGNRCRNLGTLGVGATRNLNYRIRVTWRAQRGVKLPVTFVLRSNNTVVRQAVVQLRR
                     KGR"
     gene            complement(2381..>4313)
                     /locus_tag="P5138_02325"
     CDS             complement(2381..>4313)
                     /locus_tag="P5138_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRV59442.1"
                     /translation="AARIIKGEVPEMLRGKQIYTLDLAALVAGSKYRGEFEERLKKVM
                     KEIASRGDIVLFIDEIHNLVGAGAAEGAIDAASILKPALARGEIQTIGATTLDEYRKY
                     LERDSALERRFQQIRVEQPTPEQTVKILEGLRERYEQHHKVQISDDALEAAAELADRY
                     ISDRFLPDKAIDLIDEAASRMRIKSMTAPPVYRDLEEEIETARREKEAAIEAQEFEKA
                     ANLRDTERQLTTRKRQLEEEWAEGEEGERPVLGEEEIADIVSMWTGIPVFKLTEAETK
                     KLIQMESELHKRVIGQEAAIEAVSKAIRRSRAGIKDPKRPAGSFVFLGPSGVGKTELA
                     RTLAEFLFGDEESMVRIDMSEYMEKHAVSRLVGSPPGYVGYDEGGQLTEAVRRKPYSV
                     LLLDEIEKAHPDVFNILLQILEDGRLTDSQGRTVDFRNTIIIMTSNIGASEIARNQGI
                     GFTVSDDTGITYDDMKGRIMSELRKVFRPEFLNRIDEVIVFHKLSREEVREIIELMLA
                     RVRNQVAEHELMLELDNGAKDFLADKGWDPSMGARPLRRAIQRYIEDPLADEVLKAGG
                     GDSVAGSLVTVTRDESGDDDDKPLKLKITKPRKPRKKKEDGQEKVGVGAGSTDSPSSE
                     PPAVKPDVADGPAAESAAD"
BASE COUNT          661 a         1380 c         1463 g          809 t
ORIGIN      
        1 actcgggacg ctggtggggc ccgccggaac gcccgatcga gccatacctc gagaagatcg
       61 cccggcagaa ggccgaccgg acctggcgcc acgcggactt catcgcctac cggcagccct
      121 agggcctacc ggaaatgaac ctgccacccg cggcaggttc cgaatccgcc agggagtttt
      181 tgcctagcgg cccttgcggc gaagctgaac cacggcctgc cgcaccacgg tgttgttcga
      241 gcgcaggacg aaggtgacgg gaagcttgac gccacgctgg gcgcgccagg tcacccggat
      301 ccggtaattg agatttctgg tcgccccgac cccgagcgtt cccaggttgc ggcagcggtt
      361 gccggccagc ttcagagccc gcttgcggag gttcacgcaa atcctcgtgt tgttcgacga
      421 taccccgccc aggttcgagg ccttgaccct gatccggacg accttgccct tgcgtcgggc
      481 ggcgtggccg ggctttgccg tgatcgagtt ccgggtccgg ttgaggccgg tgccggtcag
      541 gttggcgacc tcgtttccgc cgtccgaggt aaccgaaatc tgtgcggtct ggctcgcggt
      601 cgaaccgggc gtgaaagtca ccgtgaagcg gcaattggtg ttgggggtca tttccctgat
      661 gcagtcgtcg gctccgctca tcgcgtaacg atcggcgttg gctccgccca gggtgatccc
      721 ggtgaccgtg ttggtggtct gccccgtgct gcggatcgag atcgagacgc tccgcttcgt
      781 accgatgtcg gcatggccga agttgaagct gtccggatcc acgctgatcc cggggtcttc
      841 gaaggtcatc accatggccg acccgaccgg accggtgatt ccggtgaagg cggccagtgt
      901 cttgccgccg tcggtcagta cgtaacccgg atcttcgctg taggtgaagc caccggaggg
      961 cgtcaccggg tcataggaac tgttcgagaa ccgggtgaaa ttggtggtgt tctgccccgg
     1021 cagcgctccc agctcgacgc cacccgcgat gccggtgtta ttcgagttga cggcgatcgc
     1081 cggggagccg accgcggtcg cttcggtggt cgtgttggcc cagccctggc cgaccggaag
     1141 cggaaccccg ttcagcacgt tgatcatgat ccgccaggtc tcgacccgct ggccgccgcc
     1201 gtcaaggtcg tggatccagg cgatggccgg ctgcccggtg aaggactcgg ccgcgacgac
     1261 gccactgatc gggtacgggc tcagatagat ggtcggtgag gcgttggtgg ctgaagccgc
     1321 ccctcccgtg ctgagtggca cccccttggc cgtacgcagc cactggaccg accagttgta
     1381 gaaggtgttg aaggtgttga aatcgatcgt cttcttgtaa cagacccaga aggcgttcat
     1441 ggctccgtcg gagccgtagg cgatctgaat gtcgaaggga tcggcacagt ccgccccgct
     1501 cgcattcgtg tacaggagga cggtggtggt gaccgtgtca ttggccatga cgtcggcgtt
     1561 ggcgacctgg ctggtcgccg ccgagccctg gggccacatg acccggtaga agtatgggtc
     1621 gcccgccgtg gtcggagggg cggcgccgac atcgggcgga ccgggcgcag ccgaggcggc
     1681 ggacaggttg tgaaccgtcc cggaactgcc gtcggacgcg accctgacgg cctggacgac
     1741 cggctcggtg ccgtcaaagc ggagccaggc aagaccggcg gtgccgtcgg cgccgacggc
     1801 gatcgcgagg tcgctggcat cctggccggc cggcccgttg accgaccggt cggtgaccgc
     1861 cccgacggtg ccgtcggcgg cgatcgaaac cgactggatg aaatcggaag tgccgctctc
     1921 gttggtccat ccgaccaccg ccgagccgtc agcccggctg gcgataacgg gattgttggc
     1981 gttctggccg accggagaga gggtgacgat cggaccctgg gtgccatcga tctggactcg
     2041 ggtgccctgg atcaggttga aaccgcccac gtcacgcttc cagacgaccc aggaagtgcc
     2101 gtcgctgcca ccggccaccg tggtctggct gacgctggtc ccgggcccgg aaaccgtggc
     2161 cggcgaagtc cagccggcgc tcgcgagcga aggcgaagcc atcagcaggc aggcaaaggc
     2221 gaaagtcgcc agcgcgccca taatcgatcc ggtttttgca cggccatacc gcaagcgaac
     2281 cctccgtccc agttgctgtc gggtcagtct agctgtagga acaccggaaa caatcaagtg
     2341 acgcggtcgg tgaggtgaga agcggcggaa gcgacgctgc tcagtcggca gccgactcgg
     2401 cggcgggacc gtcggccacg tcaggcttga ccgccggagg ctcgctcgag ggggaatcgg
     2461 tggaaccggc cccgacaccg accttctcct gaccgtcttc cttcttcttg cgaggcttcc
     2521 gcggcttggt gatcttcagc ttgagcggct tgtcgtcatc gtcgcccgac tcgtcgcggg
     2581 ttaccgtgac cagtgacccg gccaccgaat cgccgccgcc cgccttcagc acctcgtcgg
     2641 ccagcggatc ctcgatgtag cgctggatcg cccggcggag cggtcgggcg cccatcgacg
     2701 ggtcccagcc cttgtcggcg aggaagtcct tggcgccgtt gtcgagctcg agcatcagct
     2761 cgtgctcggc cacctggttc ctgacccttg ccagcatcag ctcgatgatc tcgcgcactt
     2821 cctcgcgact cagcttgtgg aagacgatca cttcgtcgat ccggttgagg aactccggcc
     2881 ggaagacctt ccggagctcg ctcatgatgc gacccttcat gtcgtcgtag gtgatgccgg
     2941 tgtcgtcgga gaccgtgaag ccgatgccct ggttgcgggc gatctcggaa gccccgatgt
     3001 tcgaggtcat gatgatgatc gtgttgcgga agtccacggt gcggccctgc gaatcggtca
     3061 ggcggccgtc ttccaggatc tggagcagga tgttgaagac gtccgggtgg gccttctcga
     3121 tctcgtcgag cagcagcacc gagtacggct tgcggcgcac ggcctcggtc agctggccgc
     3181 cctcgtcgta gccgacgtag ccggggggcg agccaaccag acggctgacc gcatgcttct
     3241 ccatgtactc ggacatgtcg atccggacca ttgattcctc gtcgccgaag aggaactcgg
     3301 ccagggtcct cgcgagctcc gtcttgccga cgcccgatgg gccgaggaag acgaatgagc
     3361 cggccggacg cttcgggtcc ttgatgccgg cccgcgagcg acggatcgcc ttcgagacgg
     3421 cttcgatcgc ggcttcctga ccgatcacgc gcttgtgcag ctcgctctcc atctggatca
     3481 gcttcttggt ctcggcctcg gtcagcttga agaccgggat gccggtccac atcgagacga
     3541 tgtcggcgat ctcttcctcg ccgagcaccg ggcgctcgcc ctcctcgcct tcggcccact
     3601 cctcttcgag ctggcgcttg cgggtggtga gctggcgttc ggtgtcacgc aggttggcgg
     3661 ccttctcgaa ttcctgggcc tcgatcgcgg cttccttctc gcgccgggcg gtctcgatct
     3721 cttcttcgag atcgcggtag accggtggag cggtcatcga cttgatccgc atccgcgagg
     3781 cggcttcgtc aatcaggtcg atcgccttgt ccggcaggaa acggtcggag atgtaacggt
     3841 cggccagctc ggccgcggct tccagggcgt catcggagat ctggaccttg tggtgctgct
     3901 cgtagcgctc gcgcaggcct tcgaggatct tcacggtctg ttccggggtc ggctgttcga
     3961 cccggatctg ctggaagcgg cgctccaggg cagagtcgcg ttcgaggtac ttgcggtact
     4021 cgtcgagggt ggtcgcgccg atcgtctgga tctcgccacg ggccagggcc ggcttgagga
     4081 tcgaggccgc gtcaatggcg ccctcggcgg cgccggcacc gaccagattg tggatctcgt
     4141 cgatgaagag gacgatgtca ccccgggagg cgatttcctt catcactttc ttgagccgtt
     4201 cctcgaactc gccgcggtac ttggagccgg cgaccagtgc ggccaggtcc agggtgtaga
     4261 tctgcttgcc gcgcagcatc tccgggacct cgcccttgat gatgcgggcg gcc
//
LOCUS       DAOTIP010000078         4307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_24267_length_4307_cov_3.242709, whole genome shotgun sequence.
ACCESSION   DAOTIP010000078 DAOTIP010000000
VERSION     DAOTIP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24267_length_4307_cov_3.242709"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5138_02330"
     CDS             complement(<1..288)
                     /locus_tag="P5138_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRV59443.1"
                     /translation="MGKVVNIEDSQKRRSNAKKQKRSRSALVLGGGGFTGGVYEIGTL
                     RALDLLAVNSTVNDFDMYVGTSAGSFIGSMLAAGITPEEMMRVLTDEEGTPI"
     gene            360..1700
                     /gene="dacB"
                     /locus_tag="P5138_02335"
     CDS             360..1700
                     /gene="dacB"
                     /locus_tag="P5138_02335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRV59444.1"
                     /translation="MKHALPALAAAFFLAVSAPSQASPPEPLPNPDQPAVTPVFRSGG
                     SDASSSSDASASTARICPTLRRLARQGNGAAGLRVLNLKTGKDVCGLNSLARRSLASN
                     NKLFTTSTVLDRLGNRVFRTRVYAAGPVRKGVLKGNLYLKGGGDPSFGTNAFLQSYLA
                     GEGTSVERLATQIRKRGIRQITGRVFGDDTIFDRLRGVADSGYATSPWIGPLSGLSFN
                     AGYTSASLSSFSSNPAGLAARTLARLLRARGVNVRLQVGVRKTPARWVRSKGLLAWQN
                     SPDMPWMARITNLNSVNFWAETLLKDLGAYIRHKGTTAEGVKVVRRTMATLGVNVWPI
                     DGSGLTYGNRSTAYDVVKLLYKARRQPWGNYLFQSLPTAGVDGTLADRMRGSAAQRRC
                     HAKTGTLTGVSALSGYCFNQSGRRFAFSILMNDVRNLDRAHVAQDRIAALIARL"
     gene            1791..2351
                     /locus_tag="P5138_02340"
     CDS             1791..2351
                     /locus_tag="P5138_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59445.1"
                     /translation="MAERRKILLAFLLGIILATAGTATAAKLITGKDIKNGTITAKDL
                     SNSLRKKLNHTGKTGKTGATGPSGATGPAGTIDVSNFYDKGQSDSRFLKMKVVNVTGT
                     DGGSVPPFSTLTFTKLTCPAGTIPGKGTFEVIDYPANLILEDLGPTGYEVSLNNVTNT
                     TVYGTVKMALSCIGIDLSGAPEPTLP"
     gene            complement(2353..3279)
                     /locus_tag="P5138_02345"
     CDS             complement(2353..3279)
                     /locus_tag="P5138_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59446.1"
                     /translation="MSSKGGALKLLLLAALATLTFPVGSSTATMPGKNGPLLVSGYIK
                     GGLNGNTSNLFTETLNGKSKTLLGGLDQSYFDPAVSPNKRQIVYSTYPGYRMWLGPFS
                     NPKKAKAITPEESNVLNKESVFAPDGKSIYFSKTYYLDSGVYWHLKRYIIKTKKFRSY
                     KVDTKRDWGLSDVSPNGRWVAYNRGNDEDASKIRLLDTRTGKSHTVGTRGPALDPNFS
                     PDGKWLTYTAPVGDGWEVFRSRLNGKKAKRLTRGGEINFDPFFSPDGRMIAFTQGAGE
                     VKRIGIITLKTGKTRYIKAPGDYAEIEQWLRK"
     gene            complement(3290..>4307)
                     /gene="ligA"
                     /locus_tag="P5138_02350"
     CDS             complement(3290..>4307)
                     /gene="ligA"
                     /locus_tag="P5138_02350"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRV59447.1"
                     /translation="VPWAKLEPVRVGGVTVSNATLHNEEDLERKDVRAGDEVVVLRAG
                     DVIPQVISALPQKRPRGARKPHPPKTCPACGTEVVKPEDSVFSICPNRSGCPGQIFQH
                     IKHFVSRGAMDIEGLGEKQVHRFLEEGLISDVADIYDLKQEQLEALDRMGEISSGNLI
                     AEIDKSREVPFPRVLYALGLIGVGSVTAEVLADEFGSIDALHDADPERIERTEGVGPI
                     MARQIAESLADEGTWDLVERLKGAGLKMELAEDERRPEGGPLEGKTVVLTGTLPDLTR
                     DEAGAMVKRAGGKVTGSVSKKTDYVVAGESAGSKLEKAEKLGVTVLDREGLDRLLAGE
                     LPAD"
BASE COUNT          711 a         1463 c         1314 g          819 t
ORIGIN      
        1 aatcggggtg ccttcctcgt cggtcagcac acgcatcatt tcctcagggg tgatccccgc
       61 ggccagcatg ctgccgatga acgatccggc gctggttccg acgtacatgt cgaagtcgtt
      121 gaccgtcgaa ttgacggcca ggaggtcgag ggcgcgaagg gtgcctatct cgtagacgcc
      181 gccggtgaat ccgccgccgc cgaggaccag cgcagagcgc gagcgcttct gctttttggc
      241 attgctacgc cgtttctggg agtcctcgat gtttacgacc ttgcccatgc acacccaatt
      301 atgtcagcaa tccgtgtcac cttccgctac cttcgggctg cagcttgttg caatatccga
      361 tgaaacacgc cctcccggcc ctggctgcgg ccttcttcct ggccgtttca gccccctccc
      421 aggcctcccc tcccgaaccc ctgcccaatc cggaccagcc ggcggtgacc cctgtcttcc
      481 gttccggcgg aagtgacgca tccagctcgt ccgacgcaag cgccagcacg gcccggatct
      541 gcccgaccct gcgtcgcctg gcccgccagg gcaacggcgc ggcgggactg agggtcctga
      601 acctgaaaac gggcaaggac gtctgcggcc tgaactcgct ggcgcgccga tccctcgctt
      661 ccaacaacaa gctcttcacc acctcgaccg tcctcgaccg tctgggcaac cgggtcttcc
      721 ggacccgcgt ctacgcggcc ggtccggtca ggaagggtgt gctgaagggc aacctctacc
      781 tcaagggcgg gggcgacccc agcttcggca ccaacgcctt cctgcagagc tatctggccg
      841 gggagggcac atccgtcgaa cggctggcga cccagatcag gaaacggggt attcgccaga
      901 tcaccgggcg ggtcttcggc gacgacacga tcttcgaccg gctcaggggg gtcgcggatt
      961 ccggatacgc gaccagcccc tggatcggtc cgctctccgg cctttccttc aacgccggtt
     1021 acacctcggc cagtctgagc agcttcagct cgaacccggc cgggctggcc gcccgcaccc
     1081 tcgcccgact gctcagggcc cgaggcgtga acgtccggct gcaggtgggt gtgcggaaga
     1141 ccccggcccg gtgggtccgc agcaaggggc ttctcgcctg gcagaactcc cccgacatgc
     1201 cctggatggc caggatcacg aacctgaatt cggtcaactt ctgggccgag acgctgctca
     1261 aggatctcgg cgcctacatc cggcacaagg gcacgacggc agagggagtg aaggtcgtcc
     1321 gtcgcaccat ggccactctc ggtgtgaacg tgtggccgat cgacgggtcc ggactgacct
     1381 acgggaaccg ctcgaccgcg tatgacgtgg tcaagctcct ctacaaggcc cggcgccagc
     1441 cgtggggcaa ttacctgttc cagtcgctgc ctaccgccgg ggttgacggc accctggccg
     1501 accgcatgcg cggatcggcc gcccagcggc gatgccacgc caagacggga accctgaccg
     1561 gggtctcggc gctgtcgggc tactgcttca atcagtccgg ccgccgtttc gccttttcga
     1621 tcctgatgaa tgacgtgcgc aacctcgacc gcgcccacgt ggcccaggac cggatcgcgg
     1681 ccctgatcgc gcggctctga tattcgccag cctgaagtcc gttgcgggtg ggtgcgggag
     1741 ttccccgggt ttcccttgcc gttcagggct ggatcggtta ggactgaacc atggcagagc
     1801 gccgcaagat cctgcttgcg ttccttctgg ggattatcct ggccacggcg ggtactgcca
     1861 ctgccgcgaa actgatcacc ggcaaggaca tcaagaacgg gacgatcacg gcaaaggacc
     1921 tgtcgaattc gctgcgcaag aagctgaatc acacgggcaa gaccggtaag acgggagcaa
     1981 ccggaccttc gggtgcgacc gggccggccg gaacgattga cgtctccaac ttctacgaca
     2041 agggccagag cgactcccgg ttcctgaaga tgaaggtcgt gaacgtcacg ggaaccgacg
     2101 gtggctccgt tccccccttc tcgacgctca ccttcaccaa gctgacctgc ccggccggga
     2161 cgatcccggg caagggcact ttcgaagtga ttgattatcc ggccaacctg atactggagg
     2221 atctggggcc gaccggatac gaggtgtccc tgaacaacgt gaccaacacc accgtgtatg
     2281 gcacggtgaa gatggctctc agctgcatcg gcatcgatct gtcgggcgcc cccgaaccga
     2341 ccctcccctg agctacttac gcagccactg ctcgatctcg gcgtagtcgc cgggagcctt
     2401 gatgtacctg gtcttgccgg tcttcagggt gatgatcccg atccgcttga cctcgcctgc
     2461 gccctgggtg aaggcgatca tcctgccatc cggcgagaag aacgggtcga aattgatctc
     2521 cccgccccgg gtcagccgtt tcgccttctt cccgttcagg cgggacctga agacttccca
     2581 gccatcccct acgggtgccg tgtaagtgag ccacttgcca tccggcgaaa agttcgggtc
     2641 gagcgccgga cccctggtgc cgaccgtgtg gctctttccg gtgcgggtgt ccagcaacct
     2701 gatcttcgag gcgtcctcgt cgttaccccg gttgtaggca acccaccggc cgtttggcga
     2761 gacgtccgag agtccccagt cccgcttcgt atccaccttg taggacctga acttcttcgt
     2821 cttgatgatg taccgcttca gatgccagta gacgcccgag tccaggtagt aggtcttcga
     2881 gaagtagatc gacttgccat cgggggcgaa gaccgattcc ttgttcagca cgttgctttc
     2941 ctcgggggtg atcgcctttg cctttttcgg gttcgagaag gggccgagcc acatccggta
     3001 gccgggataa gtcgaataga cgatctggcg tttgtttggc gagaccgccg gatcgaagta
     3061 ggactgatcg agcccgccga gcagcgtctt cgacttgccg ttcagcgttt cggtgaagag
     3121 gttgctcgtg ttgccgttga gtccgccctt gatgtaaccc gaaaccagca gcggcccgtt
     3181 cttcccgggc atggtcgcgg tcgagctgcc aaccgggaag gtcaatgtcg cgagagctgc
     3241 gagcagcagc aacttcaggg ctcctccctt tgaactcatg gggatcaccc tagtcggcgg
     3301 gcagctctcc ggcaaggagt ctgtccagtc cttcccggtc gaggaccgtt acgccgagct
     3361 tctccgcttt ttccagcttg gagccggcgg attccccggc caccacgtag tcggtcttct
     3421 tcgagaccga tccggttacc ttgcccccgg cccgcttgac catggccccc gcctcgtccc
     3481 gggtcaggtc cggcagggtg ccggtcagga ccacggtctt gccttccagc ggaccgccct
     3541 ccggtcgtcg ttcatcttcg gccagctcca tcttcaggcc ggcacccttc agccgttcga
     3601 ccaggtccca agtgccttcg tcggccagcg actcggcgat ctgtctcgcc atgatcgggc
     3661 ccaccccttc ggtccgttcg atccgctccg ggtcggcgtc atgcagggcg tcgatcgatc
     3721 cgaactcgtc ggccagcacc tcggccgtga ccgagccgac cccgatcaga cccagcgcgt
     3781 agagcacccg cgggaaaggc acttcgcgcg acttgtcgat ctcggcgatc aggttcccgg
     3841 aggagatctc ccccatcctg tcgagcgctt ccagctgctc ctgcttcagg tcgtagatgt
     3901 cggccacatc ggagatcagc ccctcctcga ggaagcggtg gacctgcttc tcgcccaggc
     3961 cctcgatgtc catggccccg cgggagacga agtgcttgat gtgctggaag atctggccgg
     4021 gacagccgga gcggttcggg cagatcgaga agaccgagtc ctccggcttg acgacctcgg
     4081 tgccgcaggc cgggcaggtc ttcggcggat ggggtttcct cgcccctcgg ggccgcttct
     4141 gcggcagggc cgaaatcacc tgggggatca cgtcaccggc cctgagcacc accacctcgt
     4201 caccggcccg cacgtccttg cgttcgaggt cctcctcgtt gtgaagggtc gcgttggaga
     4261 ccgttacccc gccgacccgt accggttcga gcttcgccca cggaacc
//
LOCUS       DAOTIP010000079         4301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_24309_length_4301_cov_3.766133, whole genome shotgun sequence.
ACCESSION   DAOTIP010000079 DAOTIP010000000
VERSION     DAOTIP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24309_length_4301_cov_3.766133"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="P5138_02355"
     CDS             complement(<1..303)
                     /locus_tag="P5138_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRV59448.1"
                     /translation="MGLGFAAFLIIPASAYADLVFKYPQYVLTENFPTDVKIADLNGD
                     GYKDLVTANTVDDSVSVALGSNQGFTEPAENYPVGDAPEGLVVGDLNRDENLDLVTA"
     gene            complement(440..712)
                     /locus_tag="P5138_02360"
     CDS             complement(440..712)
                     /locus_tag="P5138_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRV59449.1"
                     /translation="MSAETQRLPGGVVHKLPADLRAGLVTSDVALEAWKDITPLARNE
                     FICWVEDAKKQETRERRIRRTREELEEGMRRPCCWPGCSHRERNGK"
     gene            complement(759..831)
                     /locus_tag="P5138_02365"
     tRNA            complement(759..831)
                     /locus_tag="P5138_02365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(796..798),aa:Val,seq:tac)
     gene            complement(902..1696)
                     /locus_tag="P5138_02370"
     CDS             complement(902..1696)
                     /locus_tag="P5138_02370"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRV59450.1"
                     /translation="MIPEISLGPVTLQTFGLAFAAAFVVSGWIVHMRLGEIGKPPDWA
                     YEMVFAALIGGLIGARLYFIVDNYSDVKDDLLGNIFSGSGLTWYGGALGGAIGVILWA
                     WWRKFLNLWLLDTAAPALAAGYAVGRIGCQLSGDGDYGKPWDGPWAMGYPDGTVPTPP
                     GVTVHPTPIYETLTMGLGAYILWRLRNRFRLGILFAIYLIYAGAERFLVEFLRRNTDV
                     ALGLTAAQLESLAMMIGGVVWILVVRNRYGSLARPADQMSAADVPT"
     gene            complement(1734..1805)
                     /locus_tag="P5138_02375"
     tRNA            complement(1734..1805)
                     /locus_tag="P5138_02375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1770..1772),aa:Glu,seq:ttc)
     gene            complement(1851..2744)
                     /gene="argF"
                     /locus_tag="P5138_02380"
     CDS             complement(1851..2744)
                     /gene="argF"
                     /locus_tag="P5138_02380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRV59451.1"
                     /translation="MTETRPRHFIDGGEISGGRLVTLIDRAEELRAGRPQEGGSALTG
                     KSIAMLFEKPSTRTRISFEVGTAELGGHPVVLRGSEMQLSRGESVEDTSRVLSRFVAA
                     IVIRSGSHENVVSLAEHASVPVINALTPLYHPCQALADLQTLKQRFGDLNGLKVAYVG
                     DGNNVARSLAVAGSLTGVEVVVAAPSGYQLESEVPARLTEDPIEAVSGADAIYGDVWV
                     SMGDEESAAQRRADLAPYRIDGHLLSHAKHETIVMHCLPAHPGEEITADVLYGERSAV
                     WDQAENRLHAQKALLEYLLVG"
     gene            2792..4225
                     /gene="rlmD"
                     /locus_tag="P5138_02385"
     CDS             2792..4225
                     /gene="rlmD"
                     /locus_tag="P5138_02385"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRV59452.1"
                     /translation="MSAIKPSTPPARLRRGETFEGEIETLAYGGRGIARPGGYVVFVS
                     GGLPGDRVLAEVTKSKRGYAEAVALELLAPSPDRLPNVDEHQGEACPGASWQGLAYEK
                     QLAFKEAHVADALTRIGKLGDVNIDPIIGADEQWRYRNKVEYSFGEQDGELVLGFRPQ
                     GRWDEVLDIEDCHLSSEATNQARNEVLAWARAEGLNAYDPHDHRGILRNLIVREGRRT
                     GQIQTRLVTSRGDFPRPPVDLHTVIDGPSGSTSGPTGALGEETLRERIFDLDIELSHD
                     SFFQTNTEMAEKLYGLAAEYADLTGTEKLFDLYCGVGTIGLSMARQAGQVWGLEIVPD
                     AIENAKRNAKANGITNARFVAANARTGMRPLIEEAGRPDVVTIDPPRAGLSAKIVRRL
                     IECEARRIVYISCNPTTLAPNAAQLVEAGYRLTRVRPVDMFPQTPHIECVAQFDLIGE
                     VKYVESPSDRKRRERDERRQAAKEAGQ"
BASE COUNT          772 a         1462 c         1381 g          686 t
ORIGIN      
        1 ggcggtgacc aggtcgaggt tctcatcacg gttcaggtca ccgaccacca gtccttcggg
       61 agcgtcaccg accggatagt tctcggcagg ttccgtgaac ccctggttcg aaccgagtgc
      121 gacagaaacc gaatcatcga cggtgttggc ggtgaccaag tccttgtagc cgtcgccatt
      181 gaggtcggca atcttcacgt cggtcgggaa gttctcagtc agaacgtact gggggtactt
      241 gaaaacaagg tcggcgtaag cactggccgg aatgatcagg aaagccgcaa accccagccc
      301 caacagcccc agaatcctgc gaaccgcccc agccgccctt ggatccttca tgcgtcgtgc
      361 catctgtccc ccattcctcc ggtgtgcctg acttgcctca ccctacccgg ggctttcatg
      421 tctgtccaac gacccggctc tacttgccgt ttcgctcccg gtggctgcag ccgggccagc
      481 aacagggtcg acgcatgccc tcttccagtt cctccctggt ccggcggata cgccgctccc
      541 gggtttcctg cttcttcgca tcttcgaccc agcagatgaa ctcgttccgc gccagcgggg
      601 tgatgtcctt ccacgcttcg agcgccacgt cactggtgac aagcccggcc cgcaaatcgg
      661 cgggcagctt gtgcaccacc ccaccgggca gtctctgggt ttcggcgctc ataccgaaat
      721 cgtacttggg cgcatcgatg gcctgaagac cggaaaaatg ggcggtactg ggctcgaacc
      781 agtgaccccc tgcgtgtaaa gcaggtgctc ttccaactga gctaaccgcc ccggggttcg
      841 ccgaccgggt tcaggcttgc gaaccgggat tgggcattat gcccggtcca tccggttcgg
      901 gtcaggtcgg aacgtcggcg gcagacatct ggtcggccgg ccgggcgagg ctgccatagc
      961 ggttgcggac cacaaggatc cagacgactc cgccgatcat catcgccaga ctctcgagct
     1021 gggccgccgt cagcccgagg gccacgtccg tgttgcggcg gaggaactcg accaggaacc
     1081 gctctgcgcc cgcgtagatc aggtagatcg cgaagaggat ccccagccgg aacctattcc
     1141 tcagtcgcca gaggatgtat gcgccgagcc ccatggtcag ggtctcgtag atcggggtcg
     1201 ggtggaccgt caccccgggc ggggtcggga cggtgccgtc gggatagccc atcgcccagg
     1261 ggccatccca gggcttgccg tagtcaccat cgccggagag ctggcagccg atccggccca
     1321 ccgcgtaacc ggcagcaagg gccggcgccg ctgtgtcgag cagccagagg ttcaggaact
     1381 tgcgccacca ggcccagagg atcaccccga tcgcgccacc cagggcaccg ccgtaccagg
     1441 tcagtccgga gccggaaaag atgttgccga gcaggtcgtc cttgacgtcg gagtagttgt
     1501 cgacgatgaa gtagagccgg gccccgatca ggccgccgat cagcgccgcg aacaccatct
     1561 cgtaggccca gtccggcggt ttgccgatct cgcccagccg catgtgcact atccatccgg
     1621 aaacgacgaa ggcggccgcg aaagcgagac cgaaggtctg cagggtcacg gggccgaggg
     1681 agatctccgg aatcacctgc ctgaccctac cgatgcccgt tcatcgcgag gagtagcccc
     1741 ggcgggagtc gaacccgcgc taccaggttg aaaacctgac gtgctaaccg ttacaccacg
     1801 gggccgcgcg gcggacattc tctcagttgg aggcgatcag cggcgcgccg tcaaccgacg
     1861 agcaggtact cgagcagggc cttctgggcg tggagccggt tctcggcctg gtcccagacc
     1921 gcgctgcgct ctccgtagag gacgtcggcg gtgatctcct cgccgggatg ggccggaagg
     1981 caatgcatca cgattgtctc gtgcttcgca tgtgaaagca ggtggccgtc gatccggtac
     2041 ggggcgagat cggcccgccg ctgtgcggcg ctctcctcgt cgcccatgct gacccagacg
     2101 tctccgtaga tcgcgtcggc cccgctgacc gcctcgatcg ggtcctcggt cagccttgcc
     2161 ggcacctcgg attcgagctg gtagccggac ggagccgcga cgaccacttc gaccccggtc
     2221 agtgaaccgg ccacggccag cgaacgggcg acgttgttgc cgtcacccac ataggccacc
     2281 ttcagcccat tgagatcacc gaagcgctgc ttcagcgtct gcaggtcggc cagggcctgg
     2341 caggggtggt agagcggggt cagggcgttg atcaccggca cgctcgcgtg ctcggccagg
     2401 gaaaccacat tctcgtggct gcccgaacgg atcacgatcg ccgccacgaa acgcgaaagc
     2461 acccgggagg tgtcctcgac ggactcgccg cgcgagagct gcatctcgct gcctcgcaga
     2521 accaccggat ggccgccgag ttccgccgtg ccgacctcga aagagatccg ggtcctggtc
     2581 gaaggcttct cgaagagcat cgcgatcgac ttgccggtca gagccgagcc gccctcctgc
     2641 gggcgcccgg ccctgagttc ctcggcccgg tcgatcaggg tgacaagccg gccaccggag
     2701 atctcgccgc cgtcgatgaa gtgtctgggt cgcgtttcgg tcaaggggga aatactaagc
     2761 ccgctttcag cctgagatag ttgtgggtcc aatgtctgca attaagccct ccactccccc
     2821 ggcccgcctc aggcgaggcg agaccttcga aggggaaatc gaaaccctcg cctacggcgg
     2881 ccgcggcatc gcccggcccg gcggctatgt ggttttcgtg agtggcggcc tgcccggtga
     2941 ccgggtgctg gccgaggtca cgaagtccaa gcggggttac gcggaagcgg tcgccctcga
     3001 actccttgcc ccgagcccgg accggctgcc gaacgtggac gagcatcagg gcgaggcctg
     3061 ccccggagct tcctggcagg gccttgccta cgaaaagcag ctcgccttca aggaggccca
     3121 cgtcgccgac gccctgactc gcatcggcaa gctgggggat gtcaacatcg acccgatcat
     3181 cggcgccgat gagcagtggc ggtaccgcaa caaggtcgaa tactccttcg gtgaacagga
     3241 cggcgagctg gttctcggtt tccggccgca ggggcgctgg gacgaggtgc tggacatcga
     3301 ggactgccac ctttcctccg aggcgaccaa ccaggcccgc aacgaggtac tcgcctgggc
     3361 ccgggccgag ggcctgaatg cctacgaccc gcatgaccat cgcggcatcc tgcgcaacct
     3421 gatcgtgcgg gaaggccgcc ggaccggcca gatccagacc cgtctggtca cctccagggg
     3481 agacttcccc cggccgccgg tcgatctcca cacggtcatc gacgggccct ccggctcgac
     3541 ctcgggtccg accggcgcgc tcggcgaaga gacgctgcgc gagcggatct tcgatctcga
     3601 catcgaactt tcgcatgact ccttcttcca gaccaacacc gagatggcgg agaagctcta
     3661 cggcctggcc gccgaatacg ccgacctgac cggaaccgaa aaactcttcg acctctactg
     3721 cggggtcggc acgatcggcc tcagcatggc ccgccaggcg gggcaggtct ggggcctgga
     3781 aatcgttccc gacgcgatcg agaacgcgaa acggaacgcg aaggcgaacg ggatcacgaa
     3841 cgcccgcttc gtcgccgcca acgcccgtac cgggatgcgg ccgctgatcg aggaagcggg
     3901 gcgacccgac gtggtgacga tcgacccgcc ccgggccggc ctctcggcca agatcgtgcg
     3961 acggctgatc gaatgcgagg cccgccggat cgtctacatc tcatgcaacc cgaccacgct
     4021 ggccccgaac gcggcccagc tggtcgaggc cggctatagg ctgacccgcg tgagaccggt
     4081 cgacatgttc ccgcagaccc cccacatcga atgcgtcgcc cagttcgacc tgatcggcga
     4141 ggtgaagtac gtcgaatccc ccagcgaccg caagcgtcgc gaacgagacg agcggcgaca
     4201 ggccgcgaag gaggcaggcc agtgaaccgt cccgcacaga agcgcgcctt cggagtcgcc
     4261 gccggattgc cggaagaggt ctgcggcccc ctggcagccg c
//
LOCUS       DAOTIP010000080         4239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_24753_length_4239_cov_3.413002, whole genome shotgun sequence.
ACCESSION   DAOTIP010000080 DAOTIP010000000
VERSION     DAOTIP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24753_length_4239_cov_3.413002"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="P5138_02390"
     CDS             <1..541
                     /locus_tag="P5138_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59453.1"
                     /translation="LLAADRPINGRVLVVGGVLLAFWVLGGLTVGAGRAPEASRYQYP
                     ATIFLILFMACLLDGLDLPRWLPTAVLPFALASLCLNIVQLEDGRDFLVEQTEITRAA
                     IGQVEIQGDGPRYYLNEAVTGSPYQRLIASDEYLKAERRFGSPAYSPEQIAASSAKAR
                     RAAERVIFADALARQYAGQ"
     gene            complement(557..1948)
                     /locus_tag="P5138_02395"
     CDS             complement(557..1948)
                     /locus_tag="P5138_02395"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRV59454.1"
                     /translation="MSDYATDKNTEVTMEEIVALAKRRGFVFPSSEIYGGLGSTYDYG
                     HYGVLLKNNVKNEWWKSMLVERDDIVALDSAILQHPKVWEASGHLAGFTDPLVQCLGE
                     CKRRWREDHLREANVEKGGNYDDPIVCPECGGELSEPRNFNLMFETHMGPVADDANSV
                     YLRPETAQGIFINFKNVLNFARKKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEME
                     FFVPPAEAQEWWEKWVEIRHNWYLDLGIRPDFLMIRPHEADELSHYSSGTSDVEYLFP
                     IGWSELEGIANRGNFDLTQHQEHSGESMEYFDQTSGERYIPHVIEPAAGADRAALAFL
                     VDSYLEEEVEGRKRTVLQLHPRLAPVKVAVLPLFNKEGMPETAQEINMALRKAGIQTE
                     YDSGGSIGKRYRRQDEIGTPWGITVDFDTKDDGQVTLRDRDSLEQIRVPIEGLPELIA
                     GKLAEPWKSPKLG"
     gene            2004..2375
                     /locus_tag="P5138_02400"
     CDS             2004..2375
                     /locus_tag="P5138_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRV59455.1"
                     /translation="MPIYEYRCENGHLFEVMQSMSEDALTECIECGAPVQKVLHSPAI
                     HFKGSGFHNTDYGTRKGNKAKAAAESSRKESGSSDSSGSSDSGSGSSSGSDSGSSSGS
                     GSSSGSSDSGSKASSGSSSSD"
     gene            2419..2703
                     /locus_tag="P5138_02405"
     CDS             2419..2703
                     /locus_tag="P5138_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRV59456.1"
                     /translation="MNRIGIRELRADLSNQVKRAAAGEAVVVTIDGEPKAVLTSFVAE
                     PGEMTLEELIRTGRVIPGPRVNEPRPPRPQGIKSGRPTMEVLDELREEKI"
     gene            2703..3110
                     /locus_tag="P5138_02410"
     CDS             2703..3110
                     /locus_tag="P5138_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV59457.1"
                     /translation="MLCFDSSALIKCYREEEHSPWVREVMEGDPDWCGSALLATEGPI
                     ALASSVPEVDALQVADARISRDLGFFFLVAVDADCLFRAVDIGRDFRLRTLDAIHLAA
                     ARILPSECSFLTFDDRQREAAESLGLKVIAPPV"
     gene            complement(3107..>4239)
                     /locus_tag="P5138_02415"
     CDS             complement(3107..>4239)
                     /locus_tag="P5138_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRV59458.1"
                     /translation="ADRQDLPYEVHRSGSGGGGGRSGSSGLKDRFGRGGRGGSRGGKR
                     WLKWILITCFGWFCLSVLAFGVSAQIQSMKLSGDAKDALGGSPWILTSPQNILIIGTD
                     SRPPDTLEPGAAEEERCYEQQAEGQVPHDGCPGARADTLMVVRAGGGKFKKLGIPRDS
                     YAAIPGQDTQKINAAYAFGGAALQIKTVENFLGITIDHVVILNFTGFEDFIDAIGGVK
                     VDVPVRLCADISGGEANGGWSIDLKKGSNTLDGETALAYSRTRKPSPCPGSEPSAYTE
                     GYDDLDRAAAQQLVMNGIKQRLTDPLRLPYNFIKGPIIGWTAPKAFVSDMGFFTLPQL
                     ALSSVFASSSKPDVLVPSGNGPAGSLEIPESERQRAAAALES"
BASE COUNT          717 a         1289 c         1413 g          820 t
ORIGIN      
        1 tcttctggct gccgatcggc ccataaacgg cagggtcctg gtggttggcg gggtcctgct
       61 cgccttctgg gtgctcgggg gcctgaccgt cggcgctggc agagctccgg aggcgagtcg
      121 ttaccagtac ccggcgacga tcttcctgat cctcttcatg gcctgcctgc tcgacggact
      181 cgatttgccc cgctggcttc cgacagcggt ccttcccttc gccctcgcct cgctttgcct
      241 caacattgtc cagctggaag acgggcgaga cttcctggtc gagcagaccg agatcacccg
      301 ggccgcgatc ggccaggtgg aaatccaggg ggacggtccg cgctactacc tgaacgaggc
      361 ggtgacaggg agcccgtacc agcggctgat cgcatccgat gagtacctga aggccgagag
      421 acgcttcggg tcaccggcct acagcccgga gcagatcgcg gcttccagcg cgaaggcgcg
      481 gagggccgct gagagagtga tcttcgccga cgccctcgcc cggcaatacg ccggccagta
      541 gcggggacgg aaccggtcag ccgagcttcg gggacttcca gggctcggct agcttgccgg
      601 cgatcaactc gggcaggccc tcgatcggga cccggatctg ttcgagtgag tcgcggtcac
      661 gcagggtgac ctggccgtcg tccttcgtgt cgaagtcaac ggtgattccc caaggggtgc
      721 cgatctcgtc ctgccggcgg taacgcttgc cgatcgatcc gcccgagtcg tattcggtct
      781 ggatgccggc cttccgcagc gccatgttga tctcctgcgc ggtctccggc atgccctcct
      841 tgttgaagag cggcagcacc gcgaccttga ccggggccag tcgcgggtga agctgaagca
      901 cggtgcgttt gcggccttcc acctcttcct cgaggtagga atcgaccagg aaggcgagtg
      961 cggcgcggtc ggcaccggca gccggctcga tcacatgggg gatgtaacgc tcgccggagg
     1021 tctggtcgaa gtactccatg ctctcgccgg aatgctcctg atgctgggtc aggtcgaaat
     1081 tgccccggtt ggcgatccct tccagctctg accagccgat cgggaagagg tattcgacgt
     1141 ccgaggtgcc ggacgaatag tgcgagagct cgtcggcctc atgcgggcgg atcatcagga
     1201 agtcggggcg gataccgagg tcgaggtacc agttgtgacg gatctcgacc cacttctccc
     1261 accactcctg cgcctcggcg ggcgggacga agaactccat ctccatctgt tcgaactcgc
     1321 gggtgcggaa gatgaagttg cccggggtga tctcgttgcg gaaggacttg ccgacctgag
     1381 cgatcccgaa cggcggcttc ttgcgggcga agttgaggac gttcttgaag ttgatgaaga
     1441 tgccctgggc ggtttccggc cgcaggtaga ccgagttggc gtcatcggcg accgggccca
     1501 tgtgggtttc gaacatcagg ttgaagttgc gcggctccga gagttcgccg ccgcattcgg
     1561 ggcagacgat cgggtcgtcg tagttcccgc ccttttcgac gttggcctcg cgcaggtgat
     1621 cctcgcgcca gcgtcgcttg cattcaccga ggcactggac cagggggtcg gtgaatccgg
     1681 ccagatgccc ggaggcttcc cagaccttcg ggtgctggag gatcgccgag tcgagggcga
     1741 cgatgtcatc ccgctcgacc agcatcgact tccaccactc gttcttgacg ttgttcttga
     1801 gcagaacgcc gtaatggccg tagtcgtagg tcgaaccgag gccaccgtag atctccgagg
     1861 aagggaagac gaagccgcgg cgcttggcca gcgcaacgat ctcttccatg gtcacttcgg
     1921 tgttcttgtc ggtggcgtag tcgctcatgg aggcaacggt atcgggacgg aaacgaaggc
     1981 gcgctgcgat aatcagcgtc gcaatgccga tttacgaata ccgctgtgaa aacggacacc
     2041 tcttcgaggt gatgcagtcg atgagcgagg atgccctgac cgaatgcatc gaatgcggcg
     2101 cgccggtcca gaaggtgctc cactcgcccg ccatccactt caagggctcc ggtttccaca
     2161 acaccgacta cgggacccgc aagggcaaca aggccaaggc cgcggccgag tcgtcgagga
     2221 aggaaagtgg ttcctcagac tcgtccgggt cgtccgactc cggttccggt tcttcttcgg
     2281 gctcagactc cgggtcttcg tccggttccg gctcgagttc cggctcttcg gattccggtt
     2341 cgaaggcctc ttccggctct tccagttccg actgacaaag gttcattttc cgcgtgtacc
     2401 gtggtgtaca ctcgttatat gaaccgaatc gggatcaggg aactccgtgc agacctgagc
     2461 aatcaggtga agcgagccgc tgccggtgaa gctgtggtcg tcacgataga tggcgagccg
     2521 aaagccgtcc tgacgtcgtt cgtcgcggag cctggtgaga tgaccctgga agaactgatc
     2581 aggaccggcc gggtcattcc cgggcccagg gtcaacgagc ccagaccgcc tcgaccgcaa
     2641 gggatcaaga gcgggcgtcc gacgatggaa gttctggacg agctccgcga ggagaagatc
     2701 tgatgctgtg cttcgattcg tcggctctca tcaagtgtta ccgggaggaa gaacattcgc
     2761 cttgggttcg cgaagtcatg gagggtgatc ccgattggtg cggctcggct ctgctggcaa
     2821 cagaaggacc aatcgcgctg gctagctcgg tacctgaagt ggacgccttg caggtcgctg
     2881 acgccaggat cagcagggat ctggggttct tttttctcgt ggcagtcgat gcggattgtc
     2941 tctttcgggc agtcgatatc ggtcgggatt tccgcttgag gaccctggat gcgattcacc
     3001 ttgcggcggc caggatcctt ccgtcggaat gcagtttcct caccttcgac gaccgtcagc
     3061 gcgaagcagc cgaatcgctc ggcctgaagg tcatcgcccc gccggtctag ctctcgaggg
     3121 ctgctgcggc ccgctgtcgc tccgactccg ggatctccag gctgccggcg ggaccgttgc
     3181 ccgagggcac cagaacatcc ggcttcgagg agctggcgaa gaccgatgac agtgcgagct
     3241 gggggagggt gaagaagccc atgtccgaga cgaatgcctt gggggccgtc cagccgatga
     3301 tcggaccctt gatgaagttg tagggcaggc ggaggggatc ggtcaggcgc tgcttgatcc
     3361 cgttcatgac cagctgctgg gcggcggccc ggtcgaggtc gtcgtagccc tccgtgtagg
     3421 cgctcggctc ggagccgggg caggggctcg gtttgcgggt ccgggaatag gcgagggcgg
     3481 tctccccgtc gagggtgttg ctgcccttct tgaggtcgat cgaccagcca ccgttcgcct
     3541 cgccgccgga gatgtcggcg cagaggcgga ccggcacgtc caccttgact ccgccgatcg
     3601 cgtcgatgaa gtcctcgaag ccggtgaagt tgaggatgac cacatggtcg atcgtgatgc
     3661 cgaggaaatt ctcgaccgtc ttgatctgca gggccgcgcc gccgaaggcg taggccgcat
     3721 tgatcttctg ggtgtcctgg ccggggatcg cggcgtaact gtcgcgggga atgccgagct
     3781 tcttgaactt gccgccgccg gcccggacca ccatcagggt gtccgcccgg gcgcccgggc
     3841 agccgtcatg cgggacctgg ccttcagcct gctgctcgta gcagcgttcc tcttccgcag
     3901 cgccgggttc aagagtgtcc ggagggcggg agtcggtgcc gatgatcagg atgttctgcg
     3961 ggctggttag gatccaggga ctgccgccca gggcgtcctt ggcatcaccc gaaagcttca
     4021 tcgactggat ctgggccgaa accccgaagg ccagcacgct caggcagaac cagccgaaac
     4081 atgtgatcag tatccatttc agccagcgtt tgccgccgcg actgcccccg cggccacccc
     4141 ttccgaagcg gtctttgaga ccggaagatc cggaccggcc accacctcca ccggagccgg
     4201 agcggtggac ttcgtagggt agatcctgcc tgtcggctc
//
LOCUS       DAOTIP010000081         4221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_24889_length_4221_cov_3.948872, whole genome shotgun sequence.
ACCESSION   DAOTIP010000081 DAOTIP010000000
VERSION     DAOTIP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24889_length_4221_cov_3.948872"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..387)
                     /locus_tag="P5138_02420"
     CDS             complement(25..387)
                     /locus_tag="P5138_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRV59459.1"
                     /translation="MSQDPLQDQGGFFSAQEAREFAGMTRSLRKTRRDFPRGGFGDFD
                     ALELQVLVSVAELEPTSPTKVADNILVEQNSVSEPIAALTKRGLLSVEPDLGDKRRKL
                     ITVTQQGADLLREYKASL"
     gene            561..2333
                     /locus_tag="P5138_02425"
     CDS             561..2333
                     /locus_tag="P5138_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV59460.1"
                     /translation="MTKNNTPDGIVNRRQRLLAERRREKVVEALASHYMELDPDLSLS
                     KATTRAEEFAKEEGEKNPHFDGVFLATEPPFRLPARSRTCPIAEMKGIDDLPEMRWLF
                     GEMTAAKTGRPANREMVVAAFRKMSFFGRPDLLNTYREFASNPALAWAHGDPEIPSSL
                     SKLYDQLKSFSRRMPAGMVIHANLALVDRIANLKDRRTGKLLFPNAFKYCTVDGTLIE
                     ADVPQGAPRGRSQRERKVMERSITGPETRMVQFVWYSHTSGSVPEDGPVRPAEVSKKC
                     FGWKLVVICCLDTGTPITWTLIPASGDERIALRELIRSLYLLRPDFPMEYIVGDGLYS
                     MSKEIFDFIEPKYGITTVFPRVKNQSTSKKWSKTDGVPWCKHGLMKNRGTQQAWRWRT
                     RIKRGVRPGALPPLRKWEQGHIRWTCKAKYGHKCPEVRTYFKWDRHLYTFLPHMGGSK
                     KAALRSVLQARRNRSESVFSQMKHSGAGARWPNRARWATDDGMRWLTSMAMVQITARR
                     LIHLDGSYKKALDRTRDEGLLNPSDERTFYALVDGREPLASEPVEGAVIEPPETWGID
                     ADLPIDREYILPSLEEFSTKIDRE"
     gene            complement(2609..2681)
                     /locus_tag="P5138_02430"
     tRNA            complement(2609..2681)
                     /locus_tag="P5138_02430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2646..2648),aa:Ala,seq:cgc)
     gene            2792..3814
                     /locus_tag="P5138_02435"
     CDS             2792..3814
                     /locus_tag="P5138_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59461.1"
                     /translation="MGILDEAIREHLELKRQHGADESELKGLEDEAFGEAERPDAVGP
                     TPGSEDETQIISPGSSLTAPAGEGDQLDKVFEATRQREAAEQEASAREAAAREAAKAK
                     EADTSAEVPADTEATFAEHPIPEPPAPAEPESKVDPAVTEAATVESPEAKPAEPEAGA
                     PAGEEGDASEMLEAERHHLASHPTEHYDVDAAIAEEDEIDVLSESSLSDELDRALDSG
                     EVASAGQHVEYDTPAEPDSGEIDFEEADSGEMDSAELEVEDDSAEHEAVEEDSAELLY
                     EDAATEDQPAGEEEPAESEAQEGDPDFYDQDDVLEATPEFLEETPEHDQLWFEQKEPK
                     DFDFGD"
     gene            complement(3845..>4221)
                     /locus_tag="P5138_02440"
     CDS             complement(3845..>4221)
                     /locus_tag="P5138_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV59462.1"
                     /translation="ATRAFGECSQGERQRVLLARALMPNPDLLLLDEPASGLDLPSRE
                     QLVSALSSMAGDDPSLTTVTVTHHLEEIPPTSTHALLIDGGRIVSRGPIAEALTSESV
                     SACFGLPIEVRESSGRWSAAIH"
BASE COUNT          936 a         1309 c         1286 g          690 t
ORIGIN      
        1 ccatggccct tgaccgaact ggacctagag actcgctttg tactccctca ggagatccgc
       61 tccttgttgg gtaacagtga tgagcttgcg ccgcttatcg ccgaggtcgg gttccacgct
      121 cagcagccct ctcttcgtca acgccgcgat cggttcgctg accgaattct gctctaccaa
      181 gatgttgtcg gccaccttgg tcggcgaggt cggctccagt tcagctaccg agacgaggac
      241 ctggagttca agggcatcga agtctccgaa cccgcctcga ggaaagtcac gccgggtctt
      301 gcgaaggctc ctcgtcatcc cggcgaactc tctggcctcc tgggccgaga agaacccgcc
      361 ttgatcttgc aacggatctt gcgacattac atcggtttcc gatctagttt cgcctcaggt
      421 cttgtccagg aatgcctgag tgagaggggg agcaatgacg tagatccact acgccacttc
      481 gattgacccg gcccgagggc cggacccctc ttgtatgtcg atgagccagc accgcggttc
      541 tggctcagac aggaaacgtg atgacgaaaa acaacacacc ggatggaatt gtcaacaggc
      601 gccagcgact tcttgccgag aggcgtcgag aaaaggttgt ggaggctctt gccagtcact
      661 acatggaact cgaccccgat ctcagcctga gcaaggcgac caccagagct gaagagttcg
      721 cgaaagagga aggagaaaag aaccctcact ttgacggagt ctttctcgct accgaaccgc
      781 cgtttcggtt gccagccagg tcgaggacct gtccgatcgc tgagatgaaa ggaatcgacg
      841 atctgcccga aatgagatgg ctgttcggtg agatgacagc ggcgaagacc ggtaggcccg
      901 ccaatcgcga gatggtcgtc gcggccttcc gaaagatgtc gttcttcgga aggcccgacc
      961 tccttaatac ctatcgcgag ttcgcctcga acccggctct tgcctgggct catggcgacc
     1021 cggagattcc gagttcgctc tcgaagctct acgaccagct caagtcgttc tcgagacgga
     1081 tgcctgccgg catggtgatc cacgccaacc tcgccctcgt cgatcgaatc gccaacctca
     1141 aagacaggag gaccgggaag ttgctgttcc cgaacgcctt caagtactgc actgtcgacg
     1201 gaaccctcat agaggctgac gtgccgcaag gagctccgag aggaaggagc cagcgggagc
     1261 gcaaggtgat ggagagatcc atcactggtc cggaaacccg aatggttcag ttcgtgtggt
     1321 actcgcacac cagcggatcc gttccggaag acggacctgt caggccggca gaggtctcca
     1381 agaaatgctt cggttggaag ctcgtggtga tctgctgcct cgacaccggt acaccgatca
     1441 cttggaccct gatcccggca agcggagacg aacggatcgc actccgcgaa ctcatcagat
     1501 ctctctacct cctccgaccg gacttcccga tggagtacat cgtgggagac ggtctctact
     1561 ccatgtcgaa ggagatattc gacttcatcg aacccaaata cggaatcacc accgtgtttc
     1621 ctcgagttaa aaaccagtcc acgagcaaga agtggtcgaa aacggatggg gttccctggt
     1681 gcaagcacgg cctcatgaag aaccgtggaa ctcagcaggc atggagatgg cgaaccagga
     1741 tcaagcgagg ggtccggccc ggcgcactcc caccactgcg gaaatgggaa caaggccaca
     1801 tcagatggac ctgtaaggcc aagtatggcc acaagtgccc cgaggtcagg acctacttca
     1861 agtgggaccg acacctctac accttccttc cacacatggg cggaagcaag aaggcagccc
     1921 tgaggtctgt ccttcaggct cgtcgcaacc gcagcgagtc ggtcttctcc caaatgaaac
     1981 actccggggc cggcgctagg tggcccaacc gcgcccgatg ggcgaccgac gacggcatgc
     2041 ggtggctgac ctcgatggcc atggtccaaa tcactgcccg tcgcctgatc cacctcgatg
     2101 gcagctacaa gaaagcgctg gatcgaacgc gtgatgaagg tctgctgaat ccttcggacg
     2161 agcgtacgtt ctacgccctg gtcgatggtc gggagccact cgcctcggag ccagtggagg
     2221 gcgcggtcat tgagcccccc gagacctggg gaatcgatgc cgacctcccg atcgaccgtg
     2281 agtacatcct cccatcgctc gaggaattca gcaccaagat cgaccgggag tagagcccct
     2341 gcggggttct catcgaaccc caccaaggaa cacacgtccc cgtaggtcgc agcggcctgc
     2401 ggggacgtct ctttgctcag accagttcgc ccacccgggg ctgcgatttg gctcccagac
     2461 tgaggttcac cgcgaaactt ttttgggcaa cagtgcgaca aaaatggcgg gagtttccaa
     2521 gttgaatctc cctgaggccg gaatgccgct tctgaagccg aatttcgtca atcctgactt
     2581 ttcagaatga aatccctaac gcctcgaatg gagctagccg gagtcgaacc ggcgacctct
     2641 tgggtgcgat ccaagcgctc tgccaactga gctatagccc cgcgagggac aaaggatacg
     2701 gaaggtcggc gtgcaaagta gggaaagctc atgcacgacg tccgcgaaaa ccgcatagtc
     2761 tttactacta ccgttagcta ctgagagatc aatgggaatt ctcgacgagg caatccggga
     2821 acacctcgag ctgaagcgtc agcacggggc cgacgaatcc gaactcaaag gactcgagga
     2881 cgaggcattc ggcgaggccg aacgccccga cgccgtcggt ccaaccccgg gttcggaaga
     2941 cgagacccag atcatcagcc ccggctccag cctcaccgcc ccggccggcg agggcgacca
     3001 gctcgacaag gttttcgagg ccacccgcca gcgggaagcg gccgagcagg aagcctccgc
     3061 gcgggaagct gccgccaggg aagccgcaaa ggcaaaggaa gctgacacca gcgccgaagt
     3121 gcccgccgac accgaggcaa ctttcgccga gcacccgatt cccgagccgc cggcgcccgc
     3181 cgagccggaa tcgaaggtcg atccggccgt gaccgaagcc gccaccgtcg aatcacccga
     3241 ggcgaaaccg gccgagcccg aagcgggagc gcccgccggg gaagagggcg acgcgagcga
     3301 gatgctcgag gccgagcgcc atcatctggc cagccatccg acagagcact acgacgttga
     3361 cgctgcgatc gccgaagaag acgagatcga cgtcctcagc gaaagctcgc tctccgacga
     3421 acttgaccgg gccctcgaca gcggcgaagt ggcctcggcc ggccagcacg tcgagtacga
     3481 cacccccgcg gagcctgact cgggcgagat cgatttcgag gaagcggatt cgggagaaat
     3541 ggactcggcc gaactcgagg tcgaggacga ttcggccgag catgaggcgg tcgaggaaga
     3601 ctccgcagag ttgctctatg aggacgccgc aaccgaggac cagccggccg gcgaggaaga
     3661 accagccgaa agcgaagcgc aggaaggcga tcccgacttc tacgaccagg acgacgtact
     3721 cgaagcgacc ccggaattcc ttgaggagac gccggagcac gaccaactct ggttcgagca
     3781 gaaggaaccg aaggacttcg atttcggcga ctgaccggga cgcccggcgc ccccgttcaa
     3841 tcgatcagtg aatcgcggcc gaccagcggc ctgatgactc gcgcacctcg atcggcaggc
     3901 cgaaacaggc cgaaaccgat tctgaggtga gggcctcggc gatcggtccc cgggagacga
     3961 tccggccccc gtcgatcaga agcgcgtggg tgctcgtcgg cggaatctcc tccaggtgat
     4021 gggtgaccgt gaccgtggtc agggaggggt cgtccccggc catcgaggag agagcggaaa
     4081 ccagctgctc gcgggacggc aggtcgagtc ccgaggcggg ctcgtcgagc agcagcaggt
     4141 ccgggttcgg catcagcgcc cgggcgagaa gaacccgctg cctttcgccc tgggagcatt
     4201 cgccgaaggc cctggttgcc a
//
LOCUS       DAOTIP010000082         4208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_24998_length_4208_cov_2.807368, whole genome shotgun sequence.
ACCESSION   DAOTIP010000082 DAOTIP010000000
VERSION     DAOTIP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24998_length_4208_cov_2.807368"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="P5138_02445"
     CDS             complement(<1..321)
                     /locus_tag="P5138_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRV59463.1"
                     /translation="MEPTSTQTVVSSVSRELLRARDFADSRYAEPLGVDDMARAANLS
                     RAHFSEQFKRTFGVSPHAYLLTRRLERAASMLRNTDYTVAYICVSVGLTSVGSFTTSF
                     KRAYG"
     gene            442..1842
                     /locus_tag="P5138_02450"
     CDS             442..1842
                     /locus_tag="P5138_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRV59464.1"
                     /translation="MEDGPIEAKVEQVRPLASGTLKLQSDERLLKLFRRGVEAAFEEL
                     IGRYRPALVAYAGSIAGPDRAEDVVQDSLVKAHRSLQDDRDIDPRPWLYAVVRNTALN
                     DVRDNRKHRHDGLGEVAGPGATPDDVVEQRERFAAVLAAVADLPPAQRRALVDHELSG
                     FSHEEIAAELELSTGATKQLIYRARISLRNAFGAMIPLPLVAWLSENPGLVATGAAGT
                     SATGTGLMSAIGGGGAAKLAVVAVVAGGSLAGGIAVEHNQSGSKLGGESLARATEPPR
                     AAEDNSVGTESASGADDSGRSQGSGEQSMADDKSGRESGSKDDRSGEGSGDNPSRTGG
                     RESGKDGSGSSGGSLVEDGRPGGRPSGGRQPQDGNSEDESSGEGSEGGTGGGSRPGGG
                     GSDSGTRPTSGNSPGGGPGPSGGSEDFEDSIDSEKSDEPERSTAGGSGSSGSSGSGIS
                     GKSGSGDDIELRMSLD"
     gene            1918..2556
                     /locus_tag="P5138_02455"
     CDS             1918..2556
                     /locus_tag="P5138_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59465.1"
                     /translation="MKIRRRERIGVLATAILALFVIALPGAASAKDRNKDRIPDRWER
                     SHKLSLKRDQRKLDQDHDGLRNRGEWLAGTNPRDKDSDNDGVSDLKEKAGVISAWDSE
                     SGELTITLYSGGELTGTVTDQTRVHCESEDTSDDSSDDTSEDEATDDDSGEAGNLRSG
                     SGRDGPDTGREGCDGNCSVDDLAEGVEVTEAVVKYTASGKVFKELEIVPASG"
     gene            2629..3696
                     /locus_tag="P5138_02460"
     CDS             2629..3696
                     /locus_tag="P5138_02460"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRV59466.1"
                     /translation="MAEAGEHVDLAPLTSLRLGGPAERLVKVTDTAELIRLVQAADAE
                     REPVFFLAGGSNVVIADEGVPGTTIWIRTTGIEERELEDGRLSLTVAAGEPWDDLVSR
                     SVDSGLAGIECLSGIPGSVGATPVQNVGAYGQEVSSTIASVLVLDRESGQVGPMEASQ
                     CGFGYRSSRFKGDSRFLVLAVEFILDPSDESAPVAYAQLADALEVETGERVPLSVARE
                     TVLDLRRSKSMVLDPDDPDSVSAGSFFTNPLLDLAGMAELNARAEELFGPDNLPPAYP
                     APEGMAKTSAAWLIERAGFSRGFGEGPIGLSHNHTLAIVNRGGGTTAELVSFARLIAG
                     HVEEVFGVALKPEPVFVGHSW"
     gene            complement(3705..>4208)
                     /locus_tag="P5138_02465"
     CDS             complement(3705..>4208)
                     /locus_tag="P5138_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59467.1"
                     /translation="PYPPRASQTASVQESPAAGKHTKKADKKDGPKQSGSRSRNGNGE
                     SSNSDVKTSSSGGNGGGSGGGSSDGSDGGSTGGSTGGSTGGSSGDGGGSSSGGSTTTA
                     NPVKTLNETVQAKVQQASQQAAEQVAQHVPDVVPGPPAGKGPGSQGVNGAVSGVKDTV
                     GKITGKP"
BASE COUNT          746 a         1334 c         1454 g          674 t
ORIGIN      
        1 tccataggcc cgcttgaaac tcgtggtgaa cgaaccgacg ctggtgagac cgaccgagac
       61 gcaaatgtac gccaccgtgt aatccgtgtt ccgcagcatt gacgctgccc gctcgaggcg
      121 gcgggtcagc aggtaggcgt gaggtgaaac tccgaaggtc cgcttgaact gctcgctgaa
      181 atgggcccgg gagagattcg ccgctcttgc catgtcgtcg acccccagcg gttcggcgta
      241 acgggaatcg gcaaagtctc tcgcgcgcag cagttcacgc gaaacgctgg aaaccacggt
      301 ctgcgtgctt gtgggctcca cggcctgatt atcgcgtgac ttccctcgac ggctcgtgtt
      361 aaggagacag attcacaggt agctctcagg gtccccgcgt aacctgaggg gaatcgtccc
      421 ggaggaacct gagggaaatc gatggaggat gggccgatag aagcaaaggt tgaacaggta
      481 cggccactgg ccagcggcac actgaagctc cagtcggacg agcgtctgct gaagctcttc
      541 cgccggggag tcgaagctgc cttcgaggaa ctgatcggcc ggtaccggcc tgccctcgtc
      601 gcctatgcag gctcgatcgc cggtccggac cgggccgaag acgtggtgca ggattccctt
      661 gtcaaggcac accgttccct ccaggacgac cgcgacatcg acccccgtcc gtggctttac
      721 gcggtggtgc gcaataccgc cctcaatgac gtccgcgaca accggaagca ccgccacgac
      781 gggctcggcg aagtggccgg cccgggggcg acaccggatg acgtcgtcga acagcgtgaa
      841 cgcttcgcgg cggtgcttgc cgcggtggcc gacctccctc ccgcgcagcg gcgagccctg
      901 gtcgaccatg aactgagcgg tttctcgcac gaggagatcg ccgccgaact cgaactgagt
      961 acgggcgcca caaaacaatt gatctaccgg gcccggatct cgcttcgcaa cgccttcggc
     1021 gcgatgatcc ccctccccct tgtcgcctgg ctgtcagaaa atccggggct ggtcgctacc
     1081 ggggcggctg gcaccagcgc caccgggacg gggctgatgt cagcgatcgg aggcggcggc
     1141 gcggccaagc tcgcggtggt cgcagtcgtg gccggcggat cgctggccgg agggatcgca
     1201 gtcgagcaca atcagtccgg cagcaagctc ggaggcgaaa gcctggcccg ggcgaccgag
     1261 ccaccacgtg cggccgagga caattccgtc ggaacggagt ctgcttccgg agccgacgat
     1321 tcgggccgga gccagggttc tggcgaacaa agcatggccg acgacaagtc cggccgggag
     1381 tcgggcagca aggacgaccg gagcggcgag ggctctggcg acaacccgtc ccggaccggg
     1441 ggccgggagt cgggcaagga cggctccggc agttcgggag gatcgttggt cgaagatggt
     1501 cggccgggcg gtcgtccgtc gggcggcagg caacctcagg acggcaactc ggaggatgaa
     1561 tcatcgggtg aaggttcgga aggcggcacg ggcgggggaa gccgaccggg cggcggcgga
     1621 tcggattcgg gtacgcgccc gacctccggc aactcgccgg gcggcggtcc cggaccgtcg
     1681 ggcggctcgg aagacttcga ggattcgatc gactccgaga aatccgacga accggagcga
     1741 tcgacggccg gggggtcggg atcgtccgga agttccggtt cgggcatctc cggaaaatcc
     1801 ggcagcggag atgacatcga gcttcgcatg agcctcgatt gaccctgacc gcgtgacttc
     1861 ctgccctggt tcgggtctcc ggtccagaag cgcaagcaat cactcgaaag gggaagcatg
     1921 aagatcagga ggagagaaag gatcggagtc ctcgcgaccg ccatcctcgc cttgttcgtg
     1981 atcgcgctgc caggcgctgc gtcagcgaag gacaggaaca aggacaggat tccggatcgc
     2041 tgggagcgga gccacaagct ctcgctcaag cgggaccagc gcaagctcga ccaggaccat
     2101 gacgggctcc ggaatcgcgg tgagtggctg gccgggacca acccgcgcga caaggattcc
     2161 gacaacgacg gggtcagtga cctcaaggag aaggccggag tcatttcggc ctgggactcg
     2221 gagagtggcg aactcaccat cacgctctac tcgggaggcg aactgaccgg aacggtcacc
     2281 gaccagaccc gggttcactg cgaatccgag gacacatcag acgactcgtc cgatgacaca
     2341 tccgaggacg aagcaaccga tgacgattcc ggtgaagccg ggaacctccg tagcggcagc
     2401 ggtcgcgacg ggccggacac cggccgggaa ggatgtgacg ggaactgctc ggtcgacgat
     2461 ctggctgaag gcgtggaagt gaccgaagcc gtggtcaagt acacggcctc gggcaaggtc
     2521 ttcaaggaac tggagatcgt tcccgcgagc ggttgatctg gccccccaac agatcaccac
     2581 gggaaagggg ccgcacaagc ggcccctttc ttgtggcagc atcgtggcgt ggccgaagcc
     2641 ggagaacacg ttgacctcgc cccgctgact tccctcaggc tcgggggccc ggccgaacgg
     2701 ctggtgaagg tgaccgacac cgcggaactg atccgcctgg ttcaggctgc cgatgcggag
     2761 cgggaaccgg tcttcttcct cgccgggggc tcgaacgtgg tgatcgcaga cgaaggcgtg
     2821 cccgggacga cgatctggat ccgcacgacc gggatcgagg aacgggaact cgaagacgga
     2881 agactttcgc tgacggtcgc ggccggagag ccgtgggacg acctggtcag ccgcagtgtc
     2941 gattcgggcc tggccgggat cgaatgcctc tcgggaatcc ccggctcggt cggggcgact
     3001 ccggtccaga acgtcggggc ctacggccag gaggtttcat cgaccatagc gtcggtcctg
     3061 gtcctcgacc gcgagtccgg gcaggtcggg cccatggaag cttcgcagtg cggtttcgga
     3121 taccgctcca gccgtttcaa gggtgactcc cgcttccttg tgctcgcggt ggagttcatc
     3181 ctcgaccctt ccgatgagtc ggctcccgtc gcctacgccc agctggccga tgccctcgag
     3241 gtcgagaccg gcgagcgcgt tccactttcg gtagcgaggg aaacggtcct cgacttgagg
     3301 cggtccaaga gcatggtgct cgacccggac gatccggatt ccgtgagcgc cgggtccttc
     3361 ttcaccaacc cgttgctgga cctggccggc atggccgagc tgaacgccag ggcggaggaa
     3421 ctcttcggcc cggacaacct gccgcccgct tacccggcac ccgagggtat ggccaagact
     3481 tcggccgcct ggctgatcga aagagccggg ttctcacgag gtttcggtga agggccgatc
     3541 ggcctttccc acaatcacac cctcgcgata gtgaaccgcg gcggtgggac caccgccgag
     3601 ctggttagct tcgctcgcct gatcgcgggc catgtcgagg aggtcttcgg ggtggcgttg
     3661 aagccggaac ccgtcttcgt cggccacagc tggtgaccgc gggctcaggg cttgccggta
     3721 attttgccga ccgtgtcctt gacccctgag acggcgccgt tcactccctg ggacccgggg
     3781 cccttcccgg cgggcgggcc cgggaccaca tcggggacat gctgggcgac ctgctcggcg
     3841 gcctgctgcg aggcctgctg aaccttggcc tgcactgtct cgttcagggt cttgaccggg
     3901 ttggccgtag ttgtggaccc gccggaagag ctgccgccgc cgtccccgct ggaaccgccg
     3961 gtcgacccgc cggtcgaccc cccggtcgac ccgccgtctg aaccatcgga cgagcccccg
     4021 ccgctcccgc ctccgttgcc gccggatgaa gaagtcttga cgtcactgtt ggaggactcg
     4081 ccgttgccgt tgcggctgcg ggaaccggac tgtttcgggc catccttctt gtcggccttc
     4141 ttcgtgtgct tgccggctgc ggggctctcc tgaaccgacg ccgtctggct ggcgcgcggc
     4201 ggatacgg
//
LOCUS       DAOTIP010000083         4158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_25401_length_4158_cov_3.130880, whole genome shotgun sequence.
ACCESSION   DAOTIP010000083 DAOTIP010000000
VERSION     DAOTIP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25401_length_4158_cov_3.130880"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..628
                     /locus_tag="P5138_02470"
     CDS             2..628
                     /locus_tag="P5138_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRV59468.1"
                     /translation="MDWEKGISEACLKEAEKDGFSFDVDPFMERFFEVQAEIMAGSYE
                     LYAEVLRRTIIKVAGEIGWEVEPSRAQFLPDSVAYWMPFREANAAMDRLAKKYNVGII
                     SNIDDKMLGISRRHLRTELDLVVTAQQVRSYKPDDTHFKECARRIGGKKGWLHIGSNY
                     DTDVAPLLKMKVPVIWVNRHGEKLAGRKKPSLEVKTFRDAVKKLGAGG"
     gene            781..1314
                     /locus_tag="P5138_02475"
     CDS             781..1314
                     /locus_tag="P5138_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59469.1"
                     /translation="MIIQTDDQNTATVRARLRGRVGGFQRVMPHSLRQIVRKGTEFRN
                     YYATSPVCSPSRASLLSGEYPWNTGIQGNSGPDGGWTGWQNHSVYRHNLATAMHAAGY
                     RTAHVGKFTNGYYDQTLGRTESVVPPGWTNWFTTSYVRGTHFYGYQVNLNGFVFGRSA
                     IPTTSRTDPASTPLPAP"
     gene            1320..2291
                     /locus_tag="P5138_02480"
     CDS             1320..2291
                     /locus_tag="P5138_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59470.1"
                     /translation="MTEFWPTGSCDYSGDVFTAAAVRVIRQDQNRPFFLQVDYQAPHG
                     DVAGPRGPQPATRNLRSAARTPLPKPRGFNERNFRDKPALIRAYAPKRMGRRDIHRLK
                     RSYRRYIESLRSVDDGVGRILKALRKSGKLQNTYIFLLSDHGLFLGEHRFDWGKFLPY
                     EDSSSPFMAVRGPDVPRGSVSREVTGNIDVPVTIMRLARVRPNYRVDGRSLRAYWRHP
                     NRRTRRAIAITIHSGQVSESSASASAHAPALRYNGFRVGQYKYIQYRRGGEELYDLAV
                     DPHELLNRIDKPRYAQVAAYMRSHLGQVVGCQGSACRVSLPKPPDPN"
     gene            complement(2416..>4158)
                     /locus_tag="P5138_02485"
     CDS             complement(2416..>4158)
                     /locus_tag="P5138_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HRV59471.1"
                     /translation="LFVSILGVALVPAAQAKQIAAYPSPGVKVASPTTQISFRGVKPR
                     ALGPIRVRGSRSGFHSGRIEAHSDGNGVSFLPAKPFYNQERVTVSSRRNTFAGTGKRS
                     RHFYTFATGLIVPKTWRPEPQVPARGATPPDWTSYKTFRYKVPDVNIKTSEPGANDGL
                     VFFAPRTNGPMIVDREGNLVYYDPGLRITDFRVQNYLGKPVLTWWRRAQVGNRVASFY
                     TILNRNYKVIKQFHAGNGYTSDPHEFTMAGPDTAFVNGFRTVIMDLRRFGGLKRTPVM
                     DNVAQEIDLKTGLVMWEWHSIGNVRINETYMPIPRKITRPFDYFHLNSIERDKDGNML
                     LSARHARALYKVNRRTGRIIWRMAGKKSDFKIGKGMYFAYQHDFRREANGTYSIFDNG
                     AGGPNLPRVSKRSKGIIFRINQKKKTTRLVRSFYHPEGILSNSQGNTQVLPDGNVFVG
                     WGDKKTCTEYNRKGDVLFDLWYSARQVTYRCYSDPWVGAPPASKIAVKSTVAGDHSRV
                     WVSWNGDTRVATWRVLAGDPGALAYAGQTPRDGFETTLPVDGHPAAFRLIGIDADGKV
                     IGRSKLNKLGDLTN"
BASE COUNT          701 a         1342 c         1340 g          775 t
ORIGIN      
        1 gatcgattgg gagaagggta tttccgaggc ttgcctgaag gaggccgaga aggacggttt
       61 cagcttcgat gtcgacccct tcatggagcg gtttttcgag gtccaggcag agatcatggc
      121 cggttcctac gaactgtatg ccgaggtcct gcggcggacg atcatcaagg tcgccggtga
      181 gatcggctgg gaagtcgaac cttcgcgggc ccagttcctg cccgacagcg tcgcctactg
      241 gatgccgttc cgcgaggcca acgctgccat ggaccggctg gcgaagaaat acaacgtcgg
      301 catcatcagc aacatcgatg acaagatgct ggggatctcc cgccggcacc tgcggaccga
      361 gctcgacctg gtcgtgaccg cccagcaggt ccgcagctac aagcccgacg acactcattt
      421 caaggaatgc gcccgccgga tcgggggcaa aaagggatgg ctgcacatcg gttcgaacta
      481 cgacaccgac gtggcgccct tgctaaagat gaaggttcct gtgatttggg taaaccgaca
      541 tggcgaaaaa ctggcaggcc gcaagaagcc ctcgctggag gtaaagacct tccgcgacgc
      601 agtgaagaag ctgggcgccg ggggctgatc atcttctccg ccctcaccca ctttcggggg
      661 aggcggttcg gttccgctcc ggcggaaccg ctgcttgctg ccgccgtctt cctcttcgtc
      721 gccgtcctcg ctttcctgat cctgccggcc gatcgggccc tggcggcccg tcccaacttc
      781 gtgatcatcc agaccgatga tcagaacacc gccaccgtgc gggcccgtct gcgtggtcgc
      841 gtgggcggct tccagcgggt gatgccgcat tcactccgcc agatcgtccg caagggaacc
      901 gagttccgca actactacgc gacctcgccg gtctgcagtc cctcccgcgc ttcgctgctg
      961 tccggtgaat acccctggaa cacgggcatc cagggcaaca gtggccccga cggcggctgg
     1021 accggctggc agaaccactc ggtttaccgg cacaacctgg ccaccgccat gcacgcggcc
     1081 ggttaccgca ccgcccacgt aggcaagttc accaacggct actacgacca gacgctggga
     1141 cgaacggagt cggtcgtccc gcccggctgg accaactggt tcacgacctc atacgtccgc
     1201 gggacccact tctacggcta ccaggtgaac ctgaacggct tcgtgttcgg ccgatcggca
     1261 atcccgacta ccagcaggac ggacccggca tcgaccccgc ttcctgctcc ctgagacgca
     1321 tcacggagtt ctggcccacc ggcagctgcg attacagcgg cgacgtcttc acggccgccg
     1381 ccgtgagagt catcaggcag gaccagaatc gtccgttctt cctgcaggtc gactatcagg
     1441 cgcctcatgg cgacgtcgcc ggtccccgcg gtccgcagcc ggcgaccagg aacctccgtt
     1501 ccgccgcccg caccccgctg cccaaaccgc gcgggttcaa tgagcggaac ttccgtgaca
     1561 agccggccct gatccgggct tacgccccaa agaggatggg caggcgcgac atccaccgtc
     1621 tcaagcggtc ctaccggcgc tacatcgagt cactcaggtc ggttgacgac ggcgtcggaa
     1681 ggatcctgaa ggcgcttcgc aagtcgggca agctccagaa cacctacatc ttcctgctct
     1741 cggaccacgg gctcttcctg ggcgagcacc gctttgactg gggcaagttc ctgccttacg
     1801 aggactcttc ctctccgttc atggcggtgc ggggaccgga cgttcctcga ggttcggtca
     1861 gccgggaagt gaccggcaac atcgacgttc cggtcacgat catgcgactc gcccgggtgc
     1921 gcccgaacta cagggtcgac ggccgctcgc tcagggccta ctggcggcat ccgaaccggc
     1981 ggacccgccg ggcgatcgcg atcacgatcc actcgggcca ggtttcggag tcgagcgcct
     2041 cggcgtcggc ccatgccccc gccctgaggt acaacggctt ccgggtcggc cagtacaagt
     2101 acatccagta caggcggggc ggcgaggagc tgtatgacct cgcggtcgat ccgcacgagc
     2161 ttctgaaccg catcgataag ccccgctacg cccaggtcgc cgcctacatg cggagccatc
     2221 tgggccaggt ggtcgggtgt cagggttcgg cctgccgggt ctcgctgccg aagccgcccg
     2281 acccgaactg attttctctg gtccgccccg cggcgggacc gtcggagcgg tggaccggac
     2341 caccatcggg tggtctggcc cgactggccg ggcgctcggc ccggccgtct tgccttgatc
     2401 agttggtcgg tttgatcagt tggtcaggtc gccgagcttg ttcagcttcg aacggccgat
     2461 caccttgccg tcggcatcga tgccgatcag gcggaaagcc gccgggtggc cgtcgaccgg
     2521 gagggtcgtc tcgaacccgt ccctcggtgt ctgcccggca taggcaagag cgcccggatc
     2581 gccggccagg acccgccagg ttgcaaccct ggtgtccccg ttccagctca cccagacacg
     2641 gctgtgatct cccgcgacgg tgctcttcac ggcgatcttc gaggccggcg gagcgcccac
     2701 ccacgggtcg ctgtaacagc ggtaggtgac ctgccgtgcc gaataccaga ggtcgaagag
     2761 cacgtcaccc ttgcggttgt actcggtgca ggtcttcttg tcgccccagc cgacgaagac
     2821 gttgccgtcg ggcagcacct gcgtgttgcc ttggctgttc gagaggatcc cttccgggtg
     2881 gtagaaggag cggaccagcc tggtcgtctt cttcttctgg ttgatgcgga agatgatccc
     2941 cttcgaccgc ttgctcaccc ggggcaggtt gggcccgccc gcaccgttgt cgaagatcga
     3001 gtaggtgccg ttggcctcgc gacggaagtc gtgctggtag gcgaagtaca tgcccttgcc
     3061 gatcttgaag tccgacttct tgccggccat ccgccagatg atccttccgg tccggcgatt
     3121 caccttgtag agcgcccggg cgtgacgggc ggagagcagc atgttgccgt ccttgtcccg
     3181 ttcgatcgag ttcaggtgga agtagtcaaa gggccgcgtg atcttgcggg gaatcggcat
     3241 gtaggtctcg ttgatccgca cgttgccgat cgaatgccat tcccacatga ccaggccggt
     3301 cttgaggtcg atttcctggg cgacgttgtc catgaccggg gtccgcttga ggccgccgaa
     3361 tcgccgcagg tccattatca ccgtgcggaa accgttcacg aaggccgtgt caggcccggc
     3421 catggtgaac tcatgcgggt cgctcgtgta tccgtttccg gcgtggaact gcttgatgac
     3481 cttgtagttc cggttgagga tcgtgtagaa gctggcgacc cggttgccca cctgggcgcg
     3541 acgccaccat gtgagcacgg gcttgcccag gtagttctgg acccggaagt cggtgatccg
     3601 caggccgggg tcgtaataga ccaggtttcc ctcgcgatcg acgatcatcg gaccgttcgt
     3661 gcgcggggcg aagaagacca ggccgtcgtt ggcgccgggc tcgctggtct tgatgttgac
     3721 gtcgggaacc ttgtaacgga aggtcttgta agaggtccag tccggcgggg ttgcgcccct
     3781 ggccggaacc tggggctcag ggcgccatgt ctttggaacg atcagtccgg tcgcgaaggt
     3841 gtagaagtgc ctggaccgct tgccggtgcc ggcgaatgtg tttcgcctcg atgagaccgt
     3901 aacccgttcc tggttgtaga acggcttggc cgggaggaaa ctgacgccgt tgccgtcgga
     3961 gtgcgcctcg atgcggccac tgtggaatcc gctgcgtgat ccgcgcaccc tgatcgggcc
     4021 gagggcgcgg ggcttgaccc cgcggaacga gatctgggtc gtcggagaag cgaccttcac
     4081 tccgggactg ggatatgcag ctatctgctt ggcttgggcg gccggtacga gggcgacacc
     4141 caggatggag acgaacag
//
LOCUS       DAOTIP010000084         4149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_25460_length_4149_cov_3.481192, whole genome shotgun sequence.
ACCESSION   DAOTIP010000084 DAOTIP010000000
VERSION     DAOTIP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25460_length_4149_cov_3.481192"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="P5138_02490"
     CDS             <1..768
                     /locus_tag="P5138_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV59472.1"
                     /translation="GQIMDKIRENGDFDDSLIVLTADHGISFDAADLRRHAAASNLGA
                     TLNPPLIIKYPGQVNPVVSSRSTQSIDILPTIARQLGATLAATDGQPIDEATRNRVMT
                     VGKDASRQIQVTASQIRADRRGVLRSQYRRLGNGDLWHLGPRRGLIGRQVRSAPALAG
                     TRHVLYLSRARIRRADASDNVVPALVSGRLAGIRPGRFLALAWNGRIVGTTRTFRFRR
                     KVQFGVMVPPRVMKKGRNRVTVLVNGRGGGLRRVPGR"
     gene            842..2482
                     /locus_tag="P5138_02495"
     CDS             842..2482
                     /locus_tag="P5138_02495"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRV59473.1"
                     /translation="MRYPVEIFTAEEQTLLAPYFTNLDQPVFALTNLPETVKGALFAR
                     YSRYGGTLRRLYLDEFAADAAAADPEGHLKPFDGEEGQRAARLYESVFVGFGDDSIAQ
                     LGGAHVACEWVSNILTKVLQRGRLAAYLEQSTRYIAYDQEMPGGGYRYLRGDALDDRF
                     EGAMDEIFQIYSDSLSTVEQWAAERWPRGDDEPEGPWRRSIKAKSLDLLRGLLPASTL
                     SHVGIYASGQAWEQLLFRMMASPLPEARRVGGMILEELNKVIPSFVSRVGRPDRGGEW
                     ISFLEERRQATENWVERLGLADRENEDAGPTVELTRVRGSEDDLLSACLFEESDASEI
                     RIRERIARLDAREREELMAAMIGERRNRRHRPGRGFESVSYRFEIVSDYGAFRDLQRH
                     RMLTCQWQTLTPDLGAGVPDEIREAGVGGAYEKALDISRAEYERLRDAGHPEAAPYAL
                     CLGYRIRYVLDLNAREAMQLIELRSGREGHPTYRAVAQAMHDEIADVHPAVAGAMSHL
                     DTSLEPRLERMLSEIRNHHKQLAAIQTSKAPPGFSELG"
     gene            2505..3332
                     /locus_tag="P5138_02500"
     CDS             2505..3332
                     /locus_tag="P5138_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRV59474.1"
                     /translation="MLSARIPRALPCALAFLLLALMVVFAACSSDPERSEAKSGRWQP
                     KPTIAAWQFQLQGRIDLSIDAPVYEVDGFDVSGATVGRLHARGRKVICYIDVGSWENY
                     RPDAKRFPKSVIGKKYERYPNERWLDIRRFHKFAGPIKARIRMCARKGFDGLEPDNIN
                     GWENRTGFPLTARHQLRFNRWIARLAHRNGLAVGLKNDGRQARKLVGDFDFAVVEQCF
                     QYTECGQYRPFVRHGKAVFAVEYERPTSAFCGRAKKIRFSAIGKEYDLFARPWRPCS"
     gene            complement(3430..>4149)
                     /locus_tag="P5138_02505"
     CDS             complement(3430..>4149)
                     /locus_tag="P5138_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRV59475.1"
                     /translation="HDPIEGSAEALNRLAAEEKPVVFVTNSPRITPAEQARILQEHGI
                     DASQDQVVTAGQTLIELTEGAVGKGAKIYAIGTSGFLGQLQDAGFDLMSAERWSEAAA
                     VLVTAHPRFDYEELKAATMAVRAGAFLATIGRDPTMPMPDGLWPGTGSIVAAVETASG
                     GSAVVAGKPEKPIFELGLKVLGLPGEARVAMVGDRLDTDVGGAQNAGIDGILVTGNDS
                     WAPGTKVTPNHEIESLLALVK"
BASE COUNT          678 a         1391 c         1348 g          732 t
ORIGIN      
        1 ggccagatca tggacaagat tcgcgagaac ggcgacttcg acgattccct gatcgtcctg
       61 actgctgacc acggcatctc gttcgatgcc gccgatctca ggcgccacgc ggccgcgagc
      121 aacctcggtg ccaccctcaa cccgccgctg atcatcaagt atccgggcca ggtgaatccg
      181 gtggtttcga gcaggtccac gcagtcgatc gacattctgc cgacgatcgc ccgccagctc
      241 ggcgccacgt tggcggccac cgatggccag ccgatcgacg aggcaacacg gaaccgcgtg
      301 atgacggtag gcaaggacgc ctcgcgtcag atccaggtga cggcgagtca gatacgcgcg
      361 gaccgccgag gcgttctccg aagccagtat cggcgactcg ggaacgggga tctgtggcac
      421 ctcggacccc ggaggggcct gatcggccgc caggtacggt cagctcccgc actggcgggc
      481 acccggcatg tcctctatct ctctcgggcg cggatccgca gggccgacgc gtccgacaac
      541 gtcgtgcccg cgctcgtttc ggggaggctc gcgggaatcc ggcccggcag gttcctcgct
      601 ctcgcctgga acgggcggat cgtcgggacc acccgtactt tccgcttccg tagaaaggtt
      661 cagttcggcg tgatggtgcc gccgcgggtg atgaagaagg gccgcaaccg ggtgaccgtg
      721 ctggtcaacg gccggggtgg tggcctgcgg cgggtgcccg gtcgctgaag cccatcggac
      781 cgcttcgcca ggtgtacata accggtaggc gagcgtcggg cgcgcctgtt aggttcgacg
      841 ggtgcgctac ccggtcgaga ttttcaccgc ggaggagcag accttgctcg ctccctattt
      901 cacgaatctc gaccagcccg tgttcgcgct gaccaacctg ccggagacgg tcaagggggc
      961 ccttttcgcc cgctattcgc gctacggcgg caccctccgt cgtctctatc tggacgagtt
     1021 cgcggccgac gctgcggcgg ccgacccgga agggcacctg aagcctttcg acggtgagga
     1081 aggccagagg gccgcgcgac tctacgaaag cgtcttcgtc ggtttcggag acgattcgat
     1141 cgcccagctc ggcggtgccc acgtcgcctg cgagtgggtt tccaacatcc tcaccaaggt
     1201 gcttcaaaga ggcaggcttg ccgcctacct cgagcagtcc acccgctaca tcgcctatga
     1261 ccaggagatg ccggggggcg gctaccggta cctgcgcggt gacgccctcg acgaccgctt
     1321 cgaaggtgcg atggacgaga tcttccagat ctactccgat tcgctttcca ccgtcgaaca
     1381 gtgggcggcg gagcggtggc cgcgcggcga cgatgaaccc gagggaccct ggcgccggtc
     1441 gatcaaggcc aaatccctcg accttctccg cgggctcctg cccgcctcca ctctgagcca
     1501 tgtcggcatc tacgcctcgg gtcaggcctg ggagcagctc ctgttccgga tgatggcatc
     1561 acccctgccg gaggcccggc gggtcggcgg catgatcctc gaggaactga acaaggtgat
     1621 tcccagcttc gtctcccggg tcggtcgtcc cgaccggggc ggcgaatgga tctccttcct
     1681 cgaggagcgt cggcaggcga ccgagaactg ggtcgagcgg ctcggcctgg ccgaccgtga
     1741 gaacgaggac gccggaccca cggtggagct gaccagggtt cgcggttccg aggacgacct
     1801 gctgtcggcc tgtctcttcg aggaatccga cgcctccgag attcggatcc gggaacggat
     1861 cgcaagactc gacgcccggg agcgcgagga gctgatggcg gcaatgatcg gcgagcgccg
     1921 gaaccggcgc catcggcccg gtcgcgggtt cgagtcggtg tcctaccgct tcgagatcgt
     1981 ctcggactac ggggccttcc gcgacctcca gcgtcaccgc atgctgacct gtcagtggca
     2041 gacgctcacc ccggacctgg gggccggtgt gccggacgaa atccgtgaag cgggcgtggg
     2101 cggggcctac gagaaggccc tggacatttc ccgggcggag tacgaacgcc tgcgcgatgc
     2161 cggccacccg gaagccgcgc cctacgccct ctgtctcggc taccggatcc gctatgtcct
     2221 cgacctgaat gccagggagg cgatgcagct gatcgaactc cgatccggcc gggaaggcca
     2281 cccgacctac cgggcagtgg cccaggccat gcatgacgag atcgccgacg tgcacccggc
     2341 ggtcgccggg gcgatgagcc atctcgacac gtcgctggag ccacggctcg agcgcatgct
     2401 gtccgagatc cggaaccatc acaagcagct ggccgcaatc cagacctcga aggctccgcc
     2461 aggcttctcc gaactcggct gagttgcgcc tctaagctgt ttcgatgctc tccgcccgga
     2521 ttccccgcgc gctgccctgc gccctcgcct ttctgctgct ggcgctgatg gtcgtcttcg
     2581 cggcctgttc ttcggatccg gagagatccg aggcgaagtc cggccgctgg cagcccaagc
     2641 cgaccatcgc agcctggcaa ttccagcttc agggacggat cgacctctcg attgacgccc
     2701 cggtatacga ggtcgacggg ttcgatgttt cgggagccac cgtcggcagg ctccatgccc
     2761 gcggtcgcaa ggtgatctgt tatatcgacg tcggcagctg ggagaactac cggccggacg
     2821 cgaagcgctt cccgaaatcg gtgatcggca agaagtacga gcgctatccg aacgaaaggt
     2881 ggctggacat ccgccgcttc cacaagttcg ccggaccgat caaggcgcgg atcaggatgt
     2941 gcgcccgcaa gggtttcgac ggcctcgaac cggacaacat caacggctgg gagaaccgaa
     3001 ccggctttcc gctgaccgcc cggcaccagc ttcgcttcaa ccggtggatc gccaggctcg
     3061 ctcaccgaaa cggccttgcg gtcggcctca agaacgatgg ccgtcaggcc cgcaagctgg
     3121 tcggtgactt cgacttcgcg gtggtggaac agtgctttca gtacaccgaa tgcggccagt
     3181 accgtccctt cgtcaggcac ggcaaggcgg ttttcgcggt cgagtacgaa aggccgactt
     3241 cagccttctg cggccgggcg aagaagatcc gcttcagtgc cataggcaag gagtacgacc
     3301 tcttcgcaag gccgtggcgg ccctgttcct gaccgggcag aagttgatcg gtaccccact
     3361 cttcgtcctg tacgactgat ttccgggtca aagttcgaac cctggcgttt cgcccgaggt
     3421 gagtttcggt cacttgacca gggccaggag gctttctatt tcgtggtttg gtgtgacctt
     3481 ggttcccggg gcccaggagt cgttgccggt gacgagtatc ccgtcgattc ccgcgttctg
     3541 ggcgccgcct acgtctgtgt cgaggcgatc gccgaccatt gcgacccgcg cctcgccagg
     3601 cagccccagc accttcaggc cgagctcgaa gatcggcttc tcgggcttcc cggcgaccac
     3661 cgcgcttccg cctgaagcgg tctcgacggc ggcgacgatc gatccggtcc ccggccacag
     3721 gccgtcgggc atcggcatcg tcggatcgcg tccgatcgtg gcaaggaagg cgccggcccg
     3781 gaccgccatc gtcgccgcct tcagctcctc gtagtcgaag cgcggatgag ccgtgaccag
     3841 gacggccgcc gcctcggacc accgttcggc actcatcagg tcgaagcctg catcctgcag
     3901 ctgcccgagg aatccggaag tcccgatcgc gtagatcttc gctcccttcc cgaccgcccc
     3961 ttcggtcagc tcgatcaggg tctggccggc cgtcaccacc tggtcctggc tggcgtcgat
     4021 gccgtgctcc tgaagaattc ttgcctgctc ggccggggtg atccggggac tgttggtcac
     4081 gaagacgacg ggtttttctt ctgcggcgag cctgttcagg gcttcggccg acccctctat
     4141 cggatcgtg
//
LOCUS       DAOTIP010000085         4146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_25480_length_4146_cov_3.064043, whole genome shotgun sequence.
ACCESSION   DAOTIP010000085 DAOTIP010000000
VERSION     DAOTIP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25480_length_4146_cov_3.064043"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5138_02510"
     CDS             <1..280
                     /locus_tag="P5138_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59476.1"
                     /translation="ERIHRSNLIGMGVVPLQYPDGESTDSLGLTGEEVITIGDLQGGE
                     AKTVEVTAAREGAEPVTFTATVRLDTPNEIRYINNDGILQTVLRDLNA"
     gene            complement(308..1570)
                     /locus_tag="P5138_02515"
     CDS             complement(308..1570)
                     /locus_tag="P5138_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRV59477.1"
                     /translation="MRTLRGIALFVGLLVAGLVLAPASGSAALPSEVSVAASPPSVSW
                     PDSPNTTLEVSVSSGPEGADFQFNVEPDNWSTGTISGNPFFVEAATLEGPGNLKIEST
                     EVADPSPTSCFRGGFQGVRHSYRLTLPADSESVIRVPVRALSAELPWARRGVTFSYPS
                     GDGSRWISLYTPIAVNGPKGTWINFAVRGLPKEGGELTAGRYFRIAGKTLPSLARTKI
                     VIVAEPRMIPNGFPRVSARTLATVRTNGGGNFLTPRVRLPGDAAWVLKAIPKTGPDYD
                     DEPSCGPVITTARSSRPAHTSDLLDRYFRSISVKGTKLIGQKKVWLHFERRAMGTDPD
                     DGIDPYLEYVMMGSAGCNAMGGPFTLKNGRLRWSGQIEQTLIGCQKPIDAWLEHNLRR
                     GMKARMVRGRLVLTNKRGVKIVLRETDR"
     gene            1743..1901
                     /locus_tag="P5138_02520"
     CDS             1743..1901
                     /locus_tag="P5138_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59478.1"
                     /translation="MKCKTCRKTTWAGCGLHVDAVKRTVPADQWCPGHEKAKKPASGG
                     RFTRLFGG"
     gene            complement(2375..>4146)
                     /locus_tag="P5138_02525"
     CDS             complement(2375..>4146)
                     /locus_tag="P5138_02525"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRV59479.1"
                     /translation="QAVPLLLHGDAAFPGQGVVAENFNMQSLEGYSTGGTVHIIQDNS
                     VGFTTDPMEGRSTPYASDMAKGFNVPIIHVNADDVEACSQAMRLAMAYRDRWNRDIVI
                     DVIGYRRFGHNETDEPAYTQPLMAAEIKKHKPVSEIYTGQLIEQKVVTAEEVAEAAEK
                     RRTFLKETLDGLREKMDTGVYEDPTVTNVGTGELDRSASPPVQTAVPADRLRELNSAL
                     LEVPASFTIHRKLRKPLGRRIEALEEGGIEFAHAESLALASLITEGKHVRMTGQDTER
                     GTFSQRHLVLHDEKTGIKYAPIQHLKDADAPFELHNSPLSEIACLGFEYGYASSNPDS
                     LVIWEAQFGDFANSAQVIIDSFIASGEAKWGLTSRLTLLLPHGHEGAGPEHSSARMER
                     FLQLAAEGSMRVANPSTAAQYFHLLRRQALIRKARPLIVFTPKGLLRQTAATATLEQL
                     TEQHFHFILDDPKAVDRREKVERLVLCTGRIYHDLDAAAPREDAEKVAVARVELLYPF
                     PRQQLAELFKTYPNLKEVTWVQEEPKNMGAWATMWRRLPPIMPEGVNLEYIGRPERSS
                     PSEGYSVAHIREQERIVLTALTP"
BASE COUNT          710 a         1269 c         1425 g          742 t
ORIGIN      
        1 cgagcggatc caccgttcga acctgatcgg catgggggtg gtcccgctcc agtacccgga
       61 cggcgaatcg accgactcac tcggcctgac cggcgaagag gtgatcacaa tcggcgatct
      121 ccagggtgga gaggcgaaga cggtcgaagt gactgcagcg cgcgaaggcg ccgaaccggt
      181 gacctttact gcgacggtcc gcctcgacac acccaacgaa attcgctaca taaacaacga
      241 cggaatcctt cagaccgtcc tccgcgacct gaacgcatag gcaaccggtc gcgatccgaa
      301 gtcaggatca gcgatcggtt tcgcgcagga cgatcttcac cccgcgcttg ttggtcagga
      361 ccagccgacc cctgaccatg cgtgccttca ttccccggcg gaggttgtgt tcgagccagg
      421 cgtcgatcgg cttctggcag ccgatcagtg tctgttcgat ctggcccgac cagcgaagcc
      481 ggccgttctt cagggtgaac gggccgccca tcgcgttgca gccggcgcta cccatcatca
      541 cgtattccaa ataggggtcg atgccgtcgt cggggtcggt gcccattgcc cggcgttcga
      601 agtggagcca gacctttttc tggccgatca gcttcgttcc cttgacggag attgaccgga
      661 agtaacggtc aagcaggtcc gatgtgtggg cgggacgtga gctacgcgcg gtggtgatca
      721 cgggtccgca gctcggctcg tcgtcatagt cgggaccggt cttcgggatc gccttgagca
      781 cccaggcggc gtcaccgggc agccggaccc gcggggtcag gaagttcccg ccaccgttgg
      841 tcctcaccgt tgcgagcgtg cgggccgaaa cccgcgggaa tccgttgggg atcattcgcg
      901 gttcggcgac aatcacgatc ttcgtccggg ccagggaagg cagggtcttt cctgcgatgc
      961 ggaagtacct tccggcggtg agttccccac cctctttcgg caggccccga acggcgaagt
     1021 tgatccaggt tcccttgggg ccgttcaccg cgatcggcgt gtataggctg atccagcggc
     1081 tgccgtcgcc ggacgggtac gagaaggtga ccccgcgcct tgcccacggc agctcggccg
     1141 aaagggcccg gaccgggacc ctgatcacgc tttcgctatc ggcgggaagg gtcagccggt
     1201 acgaatgccg gactccctgg aatccgcccc tgaagcaact ggtcggggaa gggtcggcga
     1261 cttccgtcga ttcgatcttc aggttgcccg gaccctccag ggtcgccgcc tcgacgaaga
     1321 acgggttgcc ggagatggtt ccggtagacc agttgtcggg ttccacgttg aactggaagt
     1381 cggccccttc aggcccggag gaaaccgaaa cctcaagggt ggtgttcggc gagtcgggcc
     1441 aggaaaccga gggaggggaa gcggcaaccg aaacttccga cggaagggcg gccgaaccgg
     1501 aggcaggggc aagcacaaga ccggcgacaa gcagtccaac gaaaagagcg atccccctga
     1561 gagtcctcat gacaggaaaa gaatagaggg ggtacacatg tcaaccccgg gccacgcctg
     1621 cgcctcgcta tcctgagaag tctcaacaag caaaggaatt cgaggggccg tccgggggcg
     1681 gcaggcgccc ccaagtggcc agatggccag gaattgaagg agaaacacct atgtgccgac
     1741 ccgtgaaatg caagacctgt cgcaagacca cctgggccgg gtgcggtctg cacgtcgatg
     1801 cggtcaagcg caccgtgccg gccgaccagt ggtgcccggg ccatgaaaag gcgaagaagc
     1861 cggcatcggg agggcgcttc actcgcctgt tcgggggata acccgcccca cgctcagcgt
     1921 ttcctcgggc atgaatgcgc atgggtagtc cagctgttcg ctgaccagga gcaagcgaag
     1981 gcggcaaagg catctgggct ggctggcgtt gccagccagc ggagccggcc cgtccatcag
     2041 ttaatgcgag tgggccggcg ggtttcacgg ggagggcggg tggaacccgc ccgactcctg
     2101 ttaagagtgg cgggcgaggg tcgcgggagg cagggggtcc gcgcgaccct caccacgcct
     2161 gcgcgtcggt agccagggag ggatgccgac gcgaacgctt ccgggggtaa accgccggaa
     2221 gcgggctttg gaaaatttca actgggtggc aacccctccg gggcgctggt ccgatcctgc
     2281 tccttccctt ttctccctac tgctactacg gacctgcaga caagtttata cacctattgg
     2341 cgacgcgcgt caattaagcg gtttgaagcc gatttcaggg cgtgagagcg gtcaaaacga
     2401 tgcgttcctg ctcgcgaata tgcgccaccg aatagccctc cgacgggctc gatcgctcgg
     2461 ggcggccgat gtattcgagg ttcacgccct cgggcatgat cgggggcagg cgcctccaca
     2521 tggtcgccca ggcgcccatg ttcttcggct cctcctgaac ccaggtcact tccttcaggt
     2581 tcgggtaggt cttgaacagc tcggccagtt gctgccgcgg gaaggggtag agcaactcga
     2641 cccgggccac cgcgaccttt tcggcgtcct cgcggggcgc cgcggcatcg aggtcgtggt
     2701 agatccgccc ggtgcagaga accagccttt cgaccttctc gcgacggtcg acggctttcg
     2761 ggtcatcgag gatgaagtgg aagtgctgct cggtcagctg ctcgagcgtg gccgtcgccg
     2821 cggtctgccg cagcagcccc tttggggtga agacgatcag cggccgggcc ttccggatca
     2881 gagcctgacg gcgcagcagg tggaagtact gggcggcagt ggaggggttc gctacccgca
     2941 tcgagccttc cgcggccagc tggaggaacc tctccatgcg ggcactcgag tgttccgggc
     3001 cggcgccttc gtggccgtgt ggcagcagca gggtgagcct cgaggtcagg ccccacttcg
     3061 cttcgccgga ggcgatgaag ctgtcgatga tcacctgggc agagttggcg aagtcaccga
     3121 actgggcttc ccagatgacc agggagtccg ggttcgacga ggcgtagcca tattcgaagc
     3181 cgaggcaggc gatctcggag agtgggctgt tgtggagctc gaacggcgcg tccgcgtcct
     3241 tcaggtgctg gatcggcgcg tacttgatgc cggtcttctc gtcgtgcagc acgagatgcc
     3301 gctgcgagaa ggtgccccgt tcggtgtcct gaccggtcat ccgcacgtgc ttgccctcgg
     3361 tgatcagcga ggccagcgcg agggattcgg catgggcgaa ctcgattccg ccttcctcga
     3421 gcgcctcgat ccggcggccg agcggcttgc gcagcttgcg gtggatcgtg aagctggccg
     3481 gtacctcgag cagcgcggag ttgagttcgc gcagccggtc ggccgggacc gcggtctgaa
     3541 caggcgggct ggccgagcgg tcgagttccc cggtgccgac gttggtcacg gtcgggtcct
     3601 cgtagacccc ggtgtccatc ttttcccgga gaccgtcgag ggtttccttg aggaaggtgc
     3661 ggcgcttctc ggcggcctcg gccacttcct cggccgtcac caccttctgc tcgatcagct
     3721 gtccggtgta gatctcggaa accggcttgt gcttcttgat ctcggcggcc atcagcggct
     3781 gggtgtaggc cggttcgtcg gtctcgttgt ggccgaagcg gcggtagccg atcacgtcga
     3841 tcacgatgtc gcggttccag cggtcgcggt aggccatcgc aagtcgcatg gcctgggagc
     3901 aggcctccac gtcgtcggcg ttgacgtgga tgatcgggac gttgaagccc ttcgccatgt
     3961 cggaggcgta tggggtcgag cggccctcca tggggtcggt cgtgaacccg acgctgttgt
     4021 cctggatgat gtggacggtg cccccggtcg agtagccctc gagcgactgc atgttgaagt
     4081 tctcggcgac gacgccctgg cccgggaacg cggcgtcgcc gtgcaggagc agcggcacag
     4141 cttgcg
//
LOCUS       DAOTIP010000086         4139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_25539_length_4139_cov_3.495837, whole genome shotgun sequence.
ACCESSION   DAOTIP010000086 DAOTIP010000000
VERSION     DAOTIP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25539_length_4139_cov_3.495837"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="P5138_02530"
     CDS             <1..753
                     /locus_tag="P5138_02530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59480.1"
                     /translation="ETLGGTLDIEASTGYFSLNLASQGLIKSLTATDISPGMLKSLAR
                     TAKSLNVPVETVVTEAEVLPFEDESFDTVLGHAVLHHIPDLDRAFSEFFRVLRPGGRI
                     VFCGEPSRYGDRLAAVPKRTGKFVAPAWRRLIGASVLEHTEEEKADDEYNLEPEVDVH
                     AFVPADLRAIPPRSGFIDTRVNGEELVSNVWGWGMRSMESTATPDSIPPRWRNFAFRS
                     YIAFQKVDNNILEPHLPAELFYNLLLSARKPA"
     gene            1025..2794
                     /locus_tag="P5138_02535"
     CDS             1025..2794
                     /locus_tag="P5138_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59481.1"
                     /translation="MSGLNMAATRGLNFGSEVIFTYGPLGFLKTPLVWYSDLSALTFM
                     YQAATFYLFVFFMVRLFRKRVSDLVACLIAFLFVACVPGIDYAVGIAVMWAFIALTDG
                     KLSDREKWLLVVLGGVFSAAECQMKISTGPLVFLVLLLGLKGSGARIRDLSAFVSIFV
                     GSYLALWLATGQVLGDLPSYVRDSVDLFTGYSGAMSAPGVSDLAIIAIAGAAIGTTVW
                     AWFGEYPSNQNRILATLIAALVCFSFYKEGVVRAGHSHLVIGLTAIAVVWLSVPIRAR
                     LLSASVAGLVLISAISINQFPYSGQIDMLANIKSAAEGARTLLDSNRRQEIVDQGRAR
                     LRSYYAIDPEVLRQIGDQPVSVDPYEIAAAWAYDLNWSPLPVFQNYSAYTERLDEINS
                     ESISSSNGPTRILRSNISTFDSGDPVEALDGRYRGWDPPRQQITTICNFIPTLTTETW
                     QVLSRTNQRCDSPVPAGEVEARSGETVSVPAPGKQEMVFARIHGLNESGFEILKSILY
                     KPGAITATTSDGASYRLIRTTASDGLMLRSNGIEGQNHGPFAQIPQTTTLKINGATGN
                     LKIEFFRMGVAKLAADTPLGEGA"
     gene            complement(2966..>4139)
                     /locus_tag="P5138_02540"
     CDS             complement(2966..>4139)
                     /locus_tag="P5138_02540"
                     /inference="COORDINATES: protein motif:HMM:NF028011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HRV59482.1"
                     /translation="PDDYPSPDLRPFTAAEAAVRELPDFEAEGLLDGLEGHDRDARLK
                     LLTELYMEDGVDLETLKEEAAANRLALLPADLVLGRSQDKYTAREIAELAGLTVNDFQ
                     NLMAAVGLPRPKPDERRFDDADLTAARRLRRFQEAGIPTENLMAVTRQVGSSAARIAQ
                     SHRDLVATDIIEPGSDEYEAAIQLRQASLQLLPLAEQAVDYALRSHFLDQLRNQAMAI
                     GSPGLFDPSDSTEVSICFADLVGFTRLGERAELEKIGTVAKLLESVAIEVTNPEVRLV
                     KTIGDAAMLVSEDGAALFDAALRFIEVGNEAGEDLPALRVGVARGEAIKQVGDWFGPP
                     VNLASRITEAARPDSVLADKAAVDAAGEEDFRYSWVGQHRFKGLDRPVKLYRVRRK"
BASE COUNT          706 a         1356 c         1255 g          822 t
ORIGIN      
        1 gaaactctcg gtggcactct cgacatcgaa gcgagcaccg gctacttcag cctcaacctg
       61 gccagccagg gcctgatcaa gagcctcacc gccaccgata tttcccccgg catgctgaag
      121 tcgctcgccc gcaccgccaa gtccctgaac gtcccggtcg agaccgtggt caccgaagcg
      181 gaagtcctgc ctttcgagga tgagagcttc gacaccgtgc tcggccacgc ggtcctccac
      241 cacatccccg atctcgacag ggccttctcc gagttcttcc gcgtcctccg gcccggcgga
      301 cgcatcgtct tctgcggcga accctcccgt tacggcgacc ggcttgccgc ggtgccgaag
      361 cggaccggca aattcgtggc tcccgcctgg cgccggctga tcggtgccag cgtgctggaa
      421 cacaccgaag aagagaaggc cgacgatgag tacaacctgg agccggaagt cgacgtccat
      481 gccttcgtcc cggccgacct cagggcgatc ccgccccggt ccggcttcat cgacacccgg
      541 gtcaacggcg aggaactcgt ttcgaatgta tggggctggg gaatgcggtc gatggaatcg
      601 acggccaccc cggattcgat cccgccgcgc tggcgcaact tcgccttccg cagctacatc
      661 gccttccaga aggtggacaa caacatcctc gaaccccacc tccccgccga gctcttctac
      721 aacctcctcc tctcggcccg caaaccggcc tagaacgggc cagcgcccac gccgcggggc
      781 cggcgacagt ctcagcttcc agcgtcgtgc tcaggctgga gactcgaagt tcaactaggg
      841 tcgttcctga cgtcgttcgt ccattactgc gttgcctgcg cccgtcttga gaccagaagc
      901 ttctgcccaa acgagttgga tcagcttggt tcgttccaat tcgtggttcc ttgccgtcct
      961 ggttgcgttg ccgggttgga gcgcgggatt tttcccggcg ggtcacggcc tggatgcgag
     1021 ttggatgagt ggtctgaaca tggcggcaac tcgcggactc aatttcgggt ccgaggtgat
     1081 tttcacctac ggaccactcg gatttctgaa gacaccgctt gtctggtact cggacctgag
     1141 cgcgctcacg ttcatgtatc aggccgcgac cttctacctg ttcgtcttct tcatggtgcg
     1201 gctgttcaga aagcgcgttt cggaccttgt cgcatgtctg atcgcgttcc tgttcgtggc
     1261 ttgcgtaccc ggaatcgact atgccgtcgg catcgcggtc atgtgggcat tcattgcctt
     1321 gacggacggc aaactgtccg atcgggagaa atggttactt gtcgtattgg gcggtgtttt
     1381 ctcggccgca gagtgccaga tgaaaatttc gacgggtccc ctcgtgttcc tggttctcct
     1441 gttgggcctc aagggctcgg gggcaaggat tcgggatcta tccgctttcg tgtcgatctt
     1501 cgtcggctct tacctcgcac tttggctcgc cacgggacag gttctcggtg acctgccttc
     1561 ctatgtgcgt gactcagtgg acctgttcac cggttattca ggagcgatga gcgcccccgg
     1621 ggtttcagat ctggcgatca tcgccatcgc cggtgcggcc atcgggacga ccgtctgggc
     1681 ctggttcggc gaatacccca gcaaccagaa ccggattctc gcgacactga ttgccgccct
     1741 ggtttgcttt tcgttttaca aggagggcgt cgtccgggcg ggccacagcc atctcgtaat
     1801 cggccttacg gcgatagccg tggtctggtt atcggtcccg attcgggccc ggctgctgtc
     1861 agcgtcggta gctggactcg tcctgatctc ggcgatctcc atcaaccaat tcccgtattc
     1921 gggtcaaatc gacatgctcg cgaatatcaa gagcgccgct gagggcgcga ggacgctcct
     1981 tgactccaat cgacggcaag aaatcgtgga ccagggtcga gctcgcctcc gctcgtacta
     2041 cgccatcgac cccgaagttc tgcgccagat cggggaccaa ccggtttcgg ttgacccgta
     2101 cgagatagcc gcagcctggg cctacgactt gaactggtcc ccgctgccgg tcttccagaa
     2161 ctactcggcc tacacggaaa gactcgacga aatcaattca gaatccatct cttcgagcaa
     2221 tggaccgacg cgaatcctca gatccaacat ttcaaccttc gattccgggg atcccgtcga
     2281 ggcactcgac ggtcgctatc gaggctggga tccgccccgg caacagatca caaccatctg
     2341 caacttcatc ccgactttga ccaccgaaac ctggcaggtc ctctcacgca ccaatcaacg
     2401 atgcgacagt cccgttccgg caggcgaggt cgaagccagg agcggggaga cagtttcggt
     2461 gcccgcaccc ggcaagcagg agatggtgtt cgcccgcatt cacgggctca acgaaagcgg
     2521 gttcgaaatc ctgaagtcga tcctctacaa gcccggggcc atcacagcca cgacctcgga
     2581 cggggccagc tatcgcctca tacgcacgac cgcatccgac ggactgatgc tcagatcaaa
     2641 cgggatcgaa ggccagaacc acgggccctt tgcccagatt cctcaaacca cgaccctcaa
     2701 gatcaacggg gcgaccggga atctcaagat tgaattcttc aggatgggag ttgccaagtt
     2761 ggcagctgac acaccgcttg gcgagggtgc ataagtcctg acccggttgc cggcattcct
     2821 tggtgcacgt tggagctgga gtgccatttc cgagtcgttt cccacttgag cgggccgtgc
     2881 ccgggaggcg ggtacgtgag ccgtcgtttc cggccgctga cgaaggtcag cgccgtcctg
     2941 gtggttcctg gcgcgcccag gctccctact tgcggcggac tctgtagagc ttgaccgggc
     3001 ggtcgagccc cttgaagcgg tgctggccta cccatgagta cctgaagtct tcttcgccgg
     3061 ccgcgtcgac cgcggccttg tcggccagga cgctgtcggg ccgggccgct tccgtgatcc
     3121 ggctggccag gttcacgggc ggcccgaacc agtcgccgac ctgcttgatc gcctcgcccc
     3181 gcgcaacacc gactctcagg gcgggcaggt cttcgccggc ctcgttgccg acctcgatga
     3241 aacggagtgc cgcgtcgaaa agcgcggcgc catcctccga gaccagcatc gccgcgtcac
     3301 cgatcgtctt gaccagcctc acctccgggt tggtcacctc gatcgccacc gattcgagca
     3361 gcttcgcgac cgtgccgatc ttctccagct ccgcgcgttc acccaagcgg gtgaagccga
     3421 ccaggtcggc gaagcagatc gagacctcgg tgctgtcgct gggatcgaac agcccggggc
     3481 tgcctatcgc catcgcctgg ttgcgaagct ggtcaaggaa atgggagcgg agcgcgtagt
     3541 cgactgcctg ttccgcaagc ggcaggagct gcagcgaggc ctgtcggagc tggatcgcgg
     3601 cttcgtactc gtcggatccg ggttcgatga tgtcggtggc gacgagatcc cgatgcgact
     3661 gcgcgatccg ggccgccgag ctgccgacct gccgggtgac ggccatcagg ttctcggtcg
     3721 gtattcccgc ttcctggaaa cgcctgagcc gccgggctgc ggtcaggtcc gcatcgtcga
     3781 agcggcgctc gtcgggcttc ggacggggca gccccacggc cgccatcagg ttctggaaat
     3841 cgttcaccgt gaggccggcc agttcggcga tttcccgggc ggtgtacttg tcctgcgagc
     3901 ggccgaggac gaggtcggcg ggcagcaggg ccagccggtt ggcagccgcc tcttccttga
     3961 gggtttcgag atcgacgccg tcttccatgt agagctcggt cagcagcttc agccgggcgt
     4021 cccggtcatg gccctccagc ccgtccagca ggccttcggc ctcgaaatcc ggcagctccc
     4081 ggacggccgc ttcggccgcc gtgaacggcc tcaggtcggg cgagggatag tcgtcggga
//
LOCUS       DAOTIP010000087         4110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_25775_length_4110_cov_3.003946, whole genome shotgun sequence.
ACCESSION   DAOTIP010000087 DAOTIP010000000
VERSION     DAOTIP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25775_length_4110_cov_3.003946"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>617
                     /locus_tag="P5138_02545"
     CDS             61..>617
                     /locus_tag="P5138_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59483.1"
                     /translation="MESRISSWLRCYRCWSRNLEVQVHYDAIRPIDPETGIPTEGDDE
                     IQESVVQCLDCMHDQPHLTFERDRVVPIEDRWERMVAGTPWVASCTVTVGADQVEACA
                     GPEAVESLTFGAFGDAGTREFFTHVRFHKHHDDEIAVHLLVELYARNPEEASEVLNNA
                     ARGTIEITSLAEESRPPAHASGDTH"
     assembly_gap    618..627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(803..1030)
                     /locus_tag="P5138_02550"
     CDS             complement(803..1030)
                     /locus_tag="P5138_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59484.1"
                     /translation="MDFKKLGQQVGQLKKQAEDKLGDRAEPDNLKRDAKELREILAGD
                     GSLAEKAKQAKEEVIDRDKSGEGPDAGVPEK"
     gene            complement(1273..1800)
                     /locus_tag="P5138_02555"
     CDS             complement(1273..1800)
                     /locus_tag="P5138_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRV59485.1"
                     /translation="MSEENNDFTTDQAIREESGSHPYGRYLEEFEVGATYKHMPAKTV
                     TEYDDHLFCLLTMNHHPLHINDVHANASQQGRNVVVGPLVYSLAVGMSVSDFSGKAIA
                     NLATDELAHPAPVFHGDTLFCESEVLEVRESKSKLDRGLVKVQTRVYNQDGVLVCHFK
                     RTALIPRKVHGTPGE"
     gene            complement(1832..2197)
                     /locus_tag="P5138_02560"
     CDS             complement(1832..2197)
                     /locus_tag="P5138_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59486.1"
                     /translation="MASTAQMIESAVTHLQEEVPALKKLKLVFGLDLRAKGDMQTYRV
                     ELPGPKVSKGRGEDERCRVEIDRQQFNQLADDKSTIGSYRRAAETGHIRFEGDSNVAK
                     LVIRVIEAHEQRAKLKKVH"
     gene            complement(2259..3458)
                     /locus_tag="P5138_02565"
     CDS             complement(2259..3458)
                     /locus_tag="P5138_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV59487.1"
                     /translation="MSSYQEITEDQKAIIEMVHQFVDEQIIPNAEEYDHDDKFPEPIV
                     EQMKELGLFGVTIAEEYGGMGLDLSTYELIVEELSRGWISISGVVNTHFIGVYLLQQF
                     GSEEQKQKYLPKMATGEMRAAFSLSEPEVGSDVQGIKTGAKKDGDDYVIDGQKMWVTN
                     GLGSSLVFLLAKTDPGAEKAHQGMTCFITEKEPWVEENTGAYEGLNVPPKIKKMGYKG
                     VESTELVYNGYRCPAENILGGEEAGLNKGFPQMMDALEVGRVNVAARGVGIAQRALEL
                     ALQYSQERKTFGKPIANHQAIQFKLADMATKLEAARLVTRKAANMKDAGIRSDLEAGM
                     AKLIASETGKECVEECFRIHGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMVIGKK
                     LLQRHKI"
     gene            3692..>4110
                     /locus_tag="P5138_02570"
     CDS             3692..>4110
                     /locus_tag="P5138_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRV59488.1"
                     /translation="MRNPRNFFKPKAIGAPDPITEVPFGPSRMIHFFDASNEKMAAKV
                     PDMAKQADILLGNLEDAVPVDNKEAARAGLVKVAKEADLGGTPLWTRINSLESPWVLD
                     DLTTLVTEVGNKIDVIMVPKVEGPWDIHYVDQLLAQL"
BASE COUNT          680 a         1265 c         1323 g          832 t
ORIGIN      
        1 gctgacctac accgggagcg acatccgccg ccgaggtcca cgaggcgcaa agatatgacc
       61 atggagtccc gtatctcttc atggctgagg tgctaccgct gctggtcccg caacctcgag
      121 gtccaggtcc actacgacgc gatccggccg atcgacccgg aaacgggcat cccgaccgag
      181 ggggacgacg agatccagga gtcggtggtc cagtgcctcg actgcatgca tgaccagccg
      241 cacctgacct tcgagcggga ccgggtggtt ccgatcgagg accgctggga gcggatggtc
      301 gccggcaccc cctgggtcgc ctcctgcacg gtcacagtag gtgccgacca ggtcgaggcc
      361 tgcgccggtc cggaagctgt cgagtccctg accttcgggg ccttcggcga tgccggcaca
      421 cgcgagtttt tcacccacgt ccgtttccac aagcaccacg acgacgagat cgccgtccac
      481 ctccttgtcg agctctacgc gaggaacccg gaggaagcga gcgaagtcct gaacaacgct
      541 gcccggggca cgatcgagat cacctcgctg gccgaggaat cacgaccccc cgcccacgcc
      601 tcgggcgata ctcactgnnn nnnnnnncac tgacttaatt gcagtcgggc cggtcttcgc
      661 cggtcctcgg ggatcaaccc gcatcttccg cgagcgccag atgatggtcg gaagaggctc
      721 tgtctggtga tgctctgctt tcgatgggtt cggtgcgggg gtgcccgaaa agtgactcct
      781 taatcagagc gcgcagcgct cgttactttt cgggtacccc cgcatcgggc ccttcgccag
      841 acttgtcgcg gtcgatcact tcttccttgg cctgcttggc cttttcggcc agtgagccgt
      901 cgccggccag gatctcgcgc agttccttcg cgtcgcgctt caggttgtcg ggctcggccc
      961 ggtcgccaag cttgtcctcg gcctgcttct tgagctgccc cacctgctga ccaagcttct
     1021 tgaagtccat gggaaaagac tacccgcgac aagtgaggcc aaccggaagc ccccggcggg
     1081 tggatgccag gagcctccag ccgttcgctg ccgaggattt cagcgggcag tgcacagggc
     1141 gctcctgccg gtggctggcg aggaggcaag cgaaggcgtt gaagggggcg cactttggtg
     1201 cgctcccgag ccgtcgagcg tccgacgaag tcaggcgccg gcaggtgcgt cctgggcgct
     1261 ttgcccgagc tgctactccc cgggagttcc gtgaaccttg cgggggatca gcgcggtgcg
     1321 cttgaagtgg cagacgagga cgccgtcctg gttgtagacg cgggtctgaa ccttgaccag
     1381 gccgcggtcg agcttgctct tggactcgcg tacctcgaga acctcggact cgcagaagag
     1441 ggtgtcgccg tggaagacgg gagccggatg ggccagttcg tcggtggcga ggttggcgat
     1501 cgccttgccg gagaagtcgg agacggacat gccgacggcc agcgagtaga ccagcgggcc
     1561 gacgacgacg ttgcgaccct gctgcgaggc gttcgcgtga acgtcgttga tgtgcagcgg
     1621 gtgatggttc atggtcagca ggcagaagag gtgatcgtca tattcggtga cggtcttggc
     1681 cggcatgtgc ttgtacgtcg ctccgacctc gaactcctcg aggtagcggc cgtacgggtg
     1741 ggatccggat tcttcgcgga tcgcctggtc ggtggtgaag tcgttgtttt cttcgctcat
     1801 ggttggttgg gttttccttt ggttgggaac ttcagtggac tttcttcagc ttggcccgct
     1861 gttcatgcgc ctcgatgacg cggatgacca gcttcgccac attcgaatcg ccttcgaaac
     1921 ggatgtggcc tgtttctgcg gcgcgacggt aggaaccgat cgtgctcttg tcgtcggcca
     1981 gctgattgaa ctgctgacgg tcgatctcga cccggcagcg ttcgtcctct cctcttccct
     2041 tgctcacctt cggccccggc aactcgaccc ggtaggtctg catgtcgccc ttggcgcgga
     2101 gatcgaggcc gaagacgagc ttcagcttct tcagggccgg cacctcctcc tggaggtggg
     2161 tgacggcgga ttcgatcatc tgcgctgttg aggccatggc aagggtgttt acatggaaag
     2221 ggccccgacg gcaagccggg gccctctcgg gttgatgctc agatcttgtg gcgctggagc
     2281 agcttcttgc cgatcaccat gcgctggatt tccgatgtgc cttcgccgat cagaagcagc
     2341 ggagcgtcac ggtagaggcg ctcgatctca tattccttcg agtagccgta gccgccgtgg
     2401 atccggaagc actcttcgac gcattccttg ccggtctcgg aggcgatcag cttcgccatg
     2461 ccggcttcga ggtcggaccg gataccggcg tccttcatgt tggccgcctt gcgggtgacc
     2521 agacgggccg cttccagctt ggtcgccatg tcggcgagct tgaactggat cgcctggtgg
     2581 ttggcaatcg gcttgccgaa ggtcttgcgc tcctggctgt actggagggc gagctcaagg
     2641 gcgcgctggg cgataccgac gccgcgggcg gcgacattga cgcgaccgac ctcgagggcg
     2701 tccatcatct gcgggaagcc cttgttcagg ccggcttcct cgccgccgag gatgttctcg
     2761 gcggggcagc ggtagccgtt gtagacgagc tcggtggact cgacgccctt gtagcccatc
     2821 ttcttgatct tcggcggaac gttgaggccc tcgtaggcgc cggtgttctc ctcgacccac
     2881 ggctccttct cggtgatgaa gcaggtcatg ccctgatggg ccttttcggc accgggatca
     2941 gtcttggcga gcaggaagac gaggctggaa ccgagtccgt tggtgaccca catcttctgg
     3001 ccgtcgatca catagtcgtc gccgtccttc ttggcaccgg tcttgatgcc ctggacgtcg
     3061 gacccgactt ccggctcgga aagcgagaac gcggcgcgca tctcgccggt cgccatcttt
     3121 ggcaggtact tctgcttttg ctcttcgcta ccgaactgct gcagcaggta gacgccgatg
     3181 aagtgggtgt tgaccacgcc ggagatcgag atccagccgc gggaaagctc ttcgacgatc
     3241 agctcgtagg tcgagagatc gaggcccatt ccgccgtatt cctcggcgat ggttactccg
     3301 aagagaccca gttccttcat ctgttcgacg atcggttccg ggaacttgtc gtcatggtcg
     3361 tactcttcgg cgttggggat gatctgctcg tcgacgaact ggtggaccat ctcgatgatg
     3421 gccttctggt cctcggtgat ctcctggtag ctggacatgg ctcctcttgg gtttggtggc
     3481 gcagacttag gaagcgcggt gtagcgcgga aaaaggcccg cggagagcgc gggcaaaggg
     3541 cctttcagaa taccgggaat ggcgctgtga tgcggtccta gtccgcgccg aagcggctgc
     3601 cggtggggaa atccatatac gctcccgaat ggcgctgata ccgctttttc gcgccggata
     3661 gcacttttct caacaaccct caaggagaac cttgcgtaac ccccggaact tcttcaagcc
     3721 caaggcgatc ggcgcccccg atccgatcac cgaggtcccc ttcggcccct cccggatgat
     3781 ccatttcttc gacgcgtcga acgagaagat ggcggccaag gtgccggaca tggcaaagca
     3841 ggccgacatc ctgcttggca acctcgaaga cgcggtgccg gtcgacaaca aggaagccgc
     3901 ccgggccggc ctggtcaagg tcgccaagga agccgatctc ggcggaaccc cgctctggac
     3961 ccggatcaac tcgctcgaga gcccgtgggt actcgatgac ctgaccacac tcgtgaccga
     4021 ggtcggcaac aagatcgacg tgatcatggt cccgaaggtc gaaggcccct gggacatcca
     4081 ctacgtcgac cagctgctcg cccagctcga
//
LOCUS       DAOTIP010000088         4097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_25886_length_4097_cov_3.080653, whole genome shotgun sequence.
ACCESSION   DAOTIP010000088 DAOTIP010000000
VERSION     DAOTIP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25886_length_4097_cov_3.080653"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="P5138_02575"
     CDS             <1..1045
                     /locus_tag="P5138_02575"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRV59489.1"
                     /translation="KEGGDDPSALETGSKQLGILASVQTGNVEMVQSASVPTSKSSPK
                     PARNTFFGLIAGLVIGLLAAFGAERFDRRLRNPDDVEESFGLPVLGVVPETEDIAASN
                     RGEAGMHLPFAAAESFRMVRASLRYFNIDEEIKVVMVTSEVPEEGKSTVAWNLANVAA
                     LSGSALLIETDLRRPSLSRQHGISPSPGLAEYLTHQVELDATIQKKSVLGENGAGTGS
                     ELDVISAGTPPPNPSELLESKSMANFLAEVRGKYDLVVIDTAPVGVVSDSLALARHAD
                     GVLIVSRIDKSTRDSAVRLKQRLRRSNVPILGVIANGVKPTRFGGYGGYGYGLEYDYS
                     SRAPEAAPSTAGD"
     gene            1075..3021
                     /locus_tag="P5138_02580"
     CDS             1075..3021
                     /locus_tag="P5138_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRV59490.1"
                     /translation="MPESRSGVWEAAVQFVLPFILVVFVGLEAGGYDIGIRSQVGIIV
                     IWLALLGVALGLLPVARVTRAGWIGIGVLGGLALFIGLGALTWTESTERSMIEFSRVL
                     MLLGVFVLVLLVQGRDGVEWSVTGVAAGVAVICAVALASRFEPGWFNVPALPQGFSTS
                     RLSYPLEYWNGLAGLMAIGLPLLAWGAGLARNLLTRALSAASIPLVVSAIYLTASRGG
                     VIAAAAGMLILLVVIPGRIRILIGLVVPAIAVLLMYGFLNDRPELRDGNLGPIAVSQG
                     SEMFTITLLMVLVVFVVQGGIAWGMERRAEGLSNFSRDTTQIVGIIAGFFALVAVVIA
                     LGSGFVGDKWTEFKQPQVSGQTVQRFGNLNSGARYEVWKSAWHASQEEPVTGIGPGTF
                     EFWWARDGGDVQFMRDAHSLYLEFLAETGPLGFLLILLVVFGPILLAGYKAMSPGDKL
                     RRAPIGAAAAGMTAFAVAAGVDWAWEMTVLPVAFFALAASVLGPAAGARTSRTTSRFE
                     SGSWRWYSRAGFGILAAIAIAIIFVPYEGDRLVQESQASYRAGDVDKALDQADDALSW
                     QSYSATAAVQKSQLLTELGMDKPAIEAAQKAVRDEPLNWLNWYVLSEAFSAAGNSREA
                     NLAQLRASQLNPNSDAIRPAGATS"
     gene            3051..>4097
                     /locus_tag="P5138_02585"
     CDS             3051..>4097
                     /locus_tag="P5138_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59491.1"
                     /translation="MEILGFSVALLCGVLVGIGRLDVLLWVLLGVVVLGVIAWAFQVV
                     VASPRTAGFWLFGVAVFCVTWSGVRVSGGATLSDVFLLLSTPFAIAGFLDRFTSFRLP
                     WWLLSGAGLLALAIFIHEVLPQSEVLTPVQQAPAMFSFQDASGSSLSMGIRLLVALIG
                     VPLVATFLVRDWGQIRVLANLWIFGIFVSCLVAVLDFSFGTGLQVSLGSSDLSQGAFG
                     ANNYGVSGADVRYVGLTFHPVALSISSSMAVPLVLSRMFSLRKAALYLPVLLVILGAS
                     LLSGSRTSILLFFVTAAIFLVWKTSYAKPLVLWIGIFILVLFVLKYGGVSVVPERFLK
                     PGEFIASNQARVDNF"
BASE COUNT          696 a         1153 c         1292 g          956 t
ORIGIN      
        1 gaaggagggg ggagatgacc cctcggcgct ggaaaccggc tccaaacagc tcggtatcct
       61 ggccagcgtt cagaccggca atgtcgagat ggtccagtcc gccagcgttc cgacctccaa
      121 gtcatcgccc aagcccgctc gaaacacatt cttcggactc attgccggct tggtaatcgg
      181 actacttgct gcgttcggcg ccgagcggtt cgaccggcgc ctgcgtaacc cggacgacgt
      241 tgaagagtct ttcgggctgc ccgtactggg cgttgttccg gaaaccgagg acattgcggc
      301 ctcaaatcgc ggggaagccg ggatgcatct tccgttcgct gcggccgagt cgttccgaat
      361 ggtccgtgcc tcgcttcgtt atttcaacat cgacgaagag atcaaagtcg tgatggtgac
      421 ctccgaagtc ccggaggaag gcaagagcac ggtcgcctgg aatctcgcca atgtggcggc
      481 gctctccggt tcagccctgc tgatcgaaac cgacctccgt cgccccagcc tttcccgcca
      541 gcatggaatt tcgccgtcgc ccggtcttgc cgagtacctg acccaccagg ttgaactcga
      601 cgcaaccatc cagaagaaat cggtgctcgg agagaatgga gctggaaccg gatcagaact
      661 ggacgtgatc tcggccggca ccccgccgcc gaatccctcg gaactcctgg aaagcaagtc
      721 gatggcgaac ttccttgccg aagtcagggg caagtacgac ctggttgtga ttgacacggc
      781 gccggtcggc gtggtttccg attcacttgc tctggcccga catgccgacg gtgtattgat
      841 cgtgtcccgg atcgacaagt ccacgcgtga ttctgcggtc cggttgaagc agcggctgcg
      901 gcgatcaaac gtaccgatcc tgggcgtgat cgccaacgga gttaagccaa cccggtttgg
      961 tggatacggc ggctacggat atggcctgga atacgactat tcctcccggg ctccggaggc
     1021 cgcaccgtcc acggccgggg actagcccgc accggggaag ccgggagttt tcggatgcct
     1081 gaatcaagat caggggtctg ggaggccgcg gtgcagttcg tgctgccctt cattctggtc
     1141 gttttcgttg gcctggaagc aggcgggtac gacatcggaa ttcgcagcca ggtcggcatc
     1201 atcgtgattt ggctggccct gctaggggtg gcgcttggcc tgcttccggt cgcacgtgtc
     1261 actcgtgccg gctggatcgg aatcggggtt ctgggtggcc tcgctttgtt catcggtctt
     1321 ggtgccctca cctggaccga gagcaccgag agaagcatga tcgagttctc ccgggtgctg
     1381 atgctgctcg gggtattcgt gctcgtcttg ctagtccagg gccgggacgg cgtggaatgg
     1441 tcggtcaccg gggtcgcggc cggggtggcc gtgatctgtg ctgtcgcact cgcctcgaga
     1501 tttgaacccg gttggttcaa cgtccccgcg ctgccgcaag ggttctccac ttcccgcctc
     1561 agctacccgc tcgaatactg gaacggactg gccggactta tggcaatagg gctcccgcta
     1621 ctggcctggg gtgccggact cgcccgaaac ctcctgacca gagcattgtc ggcagcttcg
     1681 attccattgg ttgtctcggc tatctacctc acggcctcga gaggcggagt gattgctgct
     1741 gccgccggga tgttgatctt gctggttgtg atccccggcc gaatcagaat cctgatcgga
     1801 ctggttgtgc cggcaattgc tgtcttgctc atgtatgggt ttctcaacga tcgccccgag
     1861 ctgagggacg gcaacctcgg gccaatagca gtaagccagg gcagcgagat gttcaccatc
     1921 acccttttga tggtgcttgt ggttttcgtg gttcagggag gaatcgcctg gggaatggaa
     1981 cggcgggccg aagggttgtc caacttcagc cgtgacacga cccagattgt gggaatcatc
     2041 gcgggcttct ttgccctggt tgctgtggtg atcgcgctcg gtagcggctt cgtcggcgac
     2101 aagtggaccg agttcaagca gccgcaggtc agcgggcaaa ccgttcagcg cttcggaaat
     2161 ctgaatagcg gggcaagata cgaggtctgg aaatcggcct ggcatgcgag ccaggaggaa
     2221 ccggtaaccg gaataggccc gggaactttc gagttctggt gggcccggga cggtggtgac
     2281 gtccagttca tgcgcgacgc gcattcgctc tacctcgagt tccttgccga gactggcccg
     2341 ctgggtttcc tgctcatact gctggtcgtt ttcggtccca ttctgctcgc cgggtacaag
     2401 gcgatgtcac cgggtgacaa gctcaggcga gctccgatcg gggcagctgc cgccggcatg
     2461 actgcattcg cggtggcggc gggagtcgac tgggcatggg agatgacggt cctcccggtt
     2521 gctttcttcg ccctcgcggc gtcggtcctg ggcccggctg ccggtgcgag gacaagccgg
     2581 actacctccc ggtttgagtc gggttcatgg cgctggtatt cgcgggccgg gtttggcatc
     2641 cttgcggcga tcgcgatcgc gatcattttc gtgccatatg aaggggaccg actggtccag
     2701 gaaagccaag cgagctatcg ggccggtgac gtcgacaagg cgcttgacca ggccgatgat
     2761 gccctcagct ggcagtccta ttcggccacg gccgccgttc agaagtcgca gctcctgacc
     2821 gagctcggta tggacaagcc ggcgattgaa gcagcccaga aagctgtgcg ggacgaaccg
     2881 ctgaactggc tcaactggta cgtcctgagc gaagccttct ctgccgcggg aaacagcagg
     2941 gaagccaatc tggcccagct gcgagccagc cagctgaacc ccaactctga tgccatccgg
     3001 ccggcggggg cgaccagttg acgcagcgac agattgacat accgaagtca atggagatac
     3061 ttggcttcag cgtcgcgctg ctttgcggag tcctggttgg gattggcagg ctggacgtct
     3121 tgctctgggt tttgttggga gttgtcgttc tcggggtcat cgcgtgggct tttcaggtcg
     3181 tcgtggcttc tcccagaact gctggattct ggctctttgg cgtggcagtg ttctgtgtga
     3241 cttggagtgg tgtgcgagta agcggcggcg ccaccttgtc cgatgtattt ctgctccttt
     3301 cgaccccgtt tgccatagcc gggtttctag acagattcac ttcctttcga cttccctggt
     3361 ggttgctttc cggggcgggg ctgctggctt tggccatctt cattcacgaa gtcttgccac
     3421 aaagcgaagt cttgaccccg gttcagcagg ctccggcgat gttcagcttt caggacgctt
     3481 ccggctcaag tttgagcatg gggatcaggt tgctagtagc gctcatagga gttccactcg
     3541 ttgcgacctt cctggtgcga gattggggtc aaatcagagt gctggccaac ctctggatct
     3601 tcgggatctt tgtcagttgt ctggtggcag tattggattt ttcttttggt accggactcc
     3661 aggtttcgtt gggaagctca gacctgagcc agggggcttt cggagcaaat aactatggtg
     3721 tgtccggggc ggacgtccga tacgtgggtt taacgtttca tccggttgcc ctaagtattt
     3781 cttcctccat ggcggtccca ttagtgctgt cccgcatgtt ctctttgagg aaagcggccc
     3841 tctacctccc ggtcctattg gttattcttg gtgcctccct cttgagcgga tcacggacga
     3901 gcatactctt gttcttcgtt acagctgcaa tcttcctggt ctggaagacg tcttatgcta
     3961 agccgttggt cttgtggatt ggaatcttca ttcttgttct atttgtattg aaatatggcg
     4021 gtgtctccgt agttcctgag cgattcctca agccgggaga atttatagcc agcaatcagg
     4081 ctcgtgtaga caacttt
//
LOCUS       DAOTIP010000089         4032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_26401_length_4032_cov_3.446568, whole genome shotgun sequence.
ACCESSION   DAOTIP010000089 DAOTIP010000000
VERSION     DAOTIP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26401_length_4032_cov_3.446568"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..663
                     /locus_tag="P5138_02590"
     CDS             85..663
                     /locus_tag="P5138_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59492.1"
                     /translation="MKRSSVVTFALALATVAALLAPASPASARDEVHRCTNWSWTGPS
                     SWDAVCSKQGISISSPDGRRGIDWGFSNIFCVRANSYRQSAARFIASQRQSINGLRFV
                     RKGRLRQVGRLYFRQTFIVTAGRGRNAGKGQLVFDYAAADNTGTYCYQSSKLFMVPRG
                     DYRRGIRQLIRIYHSAGYFGPGLPKGPNGEPA"
     gene            complement(694..2193)
                     /gene="lysS"
                     /locus_tag="P5138_02595"
     CDS             complement(694..2193)
                     /gene="lysS"
                     /locus_tag="P5138_02595"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRV59493.1"
                     /translation="MSEADNQQTGDDEGQVEGFAGILVERREKLDRLRASGVNPFPHS
                     FTGREEIGEVIAAHGGLEPGAETDSVHRIAGRITGRRGHGKAAFIDLRDATGQIQVHA
                     KADVLGDAYGLLEDLDLGDFIGVEGRAIVTRRGQLSIEATSWQVLTKSLRPPPDKFHG
                     LEDTETRYRHRELDLIANEESREIFRIRARTVSEVRRWLDDHGFFEVETPVLQPLYGG
                     ALARPFVTHHNALDRQLFLRIATELYLKRCVVGGIDKVYELGKDFRNEGISMKHNPEF
                     TMLEWYEAYADYNDTAAELEELVSGVAEKVLGKAEIERDGRTISLAAPWHRVTLRDAI
                     FEHAGIDIDELTDRDALSDAIGNDDETAGWGKLVDTVFSKRVEPQLVEPTFILDYPEE
                     LSPFAKQHRDKPGKVERWEAFIGGVEIANSFTELNDPDEQRRRFEQQAAEIDRGDEEA
                     QPFDESFIEALEQGMPPTGGVGLGIDRLVMMLTGAGSLREVVLFPAMRD"
     gene            complement(2218..2697)
                     /gene="greA"
                     /locus_tag="P5138_02600"
     CDS             complement(2218..2697)
                     /gene="greA"
                     /locus_tag="P5138_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRV59494.1"
                     /translation="MSRQSEITPEGLKKLEEEIEYLSTVRRREVADRIKEARDFGDIS
                     ENAEYDDAKNEQAQLEAQIVQLEERLRRSTVVEEGSAEKGEVSFGSIVHIKDQDSGDS
                     RKFQIVGSTEADLSEGKLSSESPIGAALIGGKVNQIVTIPAPRGPEKQFKITKIEAA"
     gene            complement(2803..3702)
                     /locus_tag="P5138_02605"
     CDS             complement(2803..3702)
                     /locus_tag="P5138_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV59495.1"
                     /translation="MSSAISLSERVETWRLKGGLEEFRGRQIHACFQDGEGPLLVLLH
                     GFPTCSFDWRYLFELMPDRAMLAFDFLGFGFSDKPRDHSYTLSWQADLTEELICRHWG
                     EKPAFICAHDFGASVATELMARDLAHELRFELAGMMLFNGSILVERSNPTLAQKLLRG
                     PLGPLAARFSNEAFFRRQFASVFGPSHPLTDADATDYWSLMTWNDGNRLGHKLVSYMD
                     ERAIYADRWHGAVSRWQGDLSLAWGMEDPVAVPDILDGLLEMRPELPVVRMPEIGHYP
                     QVEDPERFARVITRALTPDRTSL"
     gene            complement(3699..>4032)
                     /locus_tag="P5138_02610"
     CDS             complement(3699..>4032)
                     /locus_tag="P5138_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRV59496.1"
                     /translation="RRAYEESNADAVMIARGSFGYPWVFTELTGQEIERPSREEIMDE
                     LAWVMDRAEEHLGEERAGRYLRKFYPWYLDQLDVPKLVRNELCQTAGIDRAREIVAGI
                     RNGEPVVA"
BASE COUNT          669 a         1322 c         1308 g          733 t
ORIGIN      
        1 atcaggctgc tgcgcctggt tcagtcgacg atcacccagc gaaacctgtg aggcaacgct
       61 gccggtgagg ttaggcttgc gaacatgaag cgctcgtccg tcgtcacttt cgcccttgcc
      121 cttgcgaccg ttgccgcgct gctggcgccg gcctcccccg cctcggcccg ggacgaggtc
      181 caccgctgca ccaactggtc ctggaccggg ccgtcgagtt gggacgcggt ctgcagcaag
      241 cagggcatca gcatctcctc gccggacggg cgccgcggaa tcgactgggg cttcagcaac
      301 atcttctgtg tccgcgcgaa cagttaccgg cagtcggcgg cgagattcat cgccagccag
      361 cgccagagca tcaacggact tcgcttcgtg cgcaagggcc ggctgcgcca ggttggccgg
      421 ctctacttcc gccagacctt catcgtcacg gcgggccggg gacggaacgc cggcaagggc
      481 cagctggtct tcgattacgc cgccgccgac aacaccggca cctactgcta ccagagctcc
      541 aagctcttca tggttccgcg tggcgactac cggcgcggca tcaggcagct gatccggatc
      601 taccattcgg ccggctactt cggaccgggc ctgcccaagg gcccgaacgg cgaaccggcc
      661 tgagggtcgg ggccacgccc cgcggccggt cgatcagtcc cgcatcgccg ggaagagaac
      721 cacttcccgg agggagccgg caccggtcag catcatcacc aggcggtcga tgccgagccc
      781 gacaccgccg gtcggcggca tgccctgttc cagcgcctcg atgaatgact cgtcgaaggg
      841 ctgggcctct tcgtcgcccc ggtcgatctc ggccgcctgc tgttcgaagc ggcgccgctg
      901 ctcgtccggg tcgttcagct cggtgaacga attggcgatc tcgacaccgc cgatgaaggc
      961 ctcccagcgc tcgaccttcc ccggcttgtc ccggtgctgc ttggcgaagg gagaaagctc
     1021 ttccgggtaa tcgaggatga aggtgggttc gaccagctgc ggctcgaccc gtttcgagaa
     1081 gaccgtgtcg accagcttgc cccagccggc ggtctcgtca tcgttgccga tcgcatccga
     1141 aagggcgtcc cggtcggtca gttcgtctat gtcgattccg gcgtgctcga agatcgcgtc
     1201 gcgcagggtg accctgtgcc agggcgcggc gaggctgatc gtccggccgt cgcgttctat
     1261 ctcggccttg ccgagcacct tttccgccac gcccgagacc agttcttcga gctctgccgc
     1321 cgtgtcgttg tagtcggcgt aggcctcgta ccactcgagc atcgtgaact cggggttgtg
     1381 tttcatcgag atgccttcgt tgcggaaatc cttgcccaac tcgtaaacct tgtcgatccc
     1441 gccgaccacg cagcgcttca ggtagagctc ggtcgcgatc cggaggaaga gctggcggtc
     1501 gagagcgttg tgatgggtga cgaacggccg ggccagggcg ccgccataga gcggctgcag
     1561 gaccggggtt tccacctcga agaatccgtg gtcgtcgagc cagcggcgca cttcggagac
     1621 cgtgcgggcc cggatccgga agatctcgcg tgactcctcg ttggcgatca ggtcgagttc
     1681 ccgatggcgg taacgggtct ccgtgtcttc gagtccgtgg aacttgtccg gtggcggacg
     1741 gagtgacttg gtcagaacct gccatgaggt cgcctcgatc gaaagctgtc cgcgccgggt
     1801 gacgatggcc cggccctcca ccccgatgaa gtcaccgagg tcgagatctt cgagcagtcc
     1861 gtaggcatcc ccgagcacgt ccgctttggc gtgaacctgg atctggccgg tggcgtcgcg
     1921 caggtcgatg aacgcggcct tgccgtggcc ccggcggccg gtgatccggc cggcgatccg
     1981 gtggacagaa tccgtctcgg cccccggctc cagcccgccg tgggccgcga ttacctcgcc
     2041 gatttcttcg cggccagtaa acgaatgcgg gaacgggttg actccggacg ccctgaggcg
     2101 atccagcttc tcccggcgct cgacgaggat gccggcgaaa ccttcgacct ggccctcgtc
     2161 gtccccggtc tgctggttat cggcttcgct catcgccggc cttccccgac ccggaaatca
     2221 ggccgcttcg atcttggtga tcttgaactg cttttccggg ccgcgcggcg ccggaatcgt
     2281 gacgatctgg ttgaccttgc caccgatcag ggcggcgccg atcggcgact cgctggagag
     2341 cttgccctcg gagaggtcgg cctcggtcga accgacaatc tggaacttgc gggaatcacc
     2401 cgagtcctgg tccttgatgt ggacgatcga accgaaggag acctcgccct tctcggccga
     2461 gccttcctcg accacggtcg aacgacgcaa ccgctcctcg agctggacga tctgggcctc
     2521 gagctgggcc tgctcgttct tggcgtcgtc gtattcggcg ttctcggaaa tgtctccgaa
     2581 gtcacgcgct tccttgatcc ggtcggcgac ctcgcgacgg cgcaccgtcg agagatactc
     2641 gatctcttcc tcgagcttct tcagaccttc aggcgtgatt tcagactggc gactcacagg
     2701 tatcaacctc tcttggggag gccggcagaa cagggccggc cctgaaaatg acgaacgccc
     2761 tgagcggcac cagacggcac cggcagggca gcggacgcgc gattataacg aggtgcgatc
     2821 gggagtcagc gcccgggtga tcacgcgggc gaagcgttcc gggtcctcga cctgcggata
     2881 atgcccgatt tccggcatcc ggacgaccgg cagttccggc cgcatttcaa gcaatccgtc
     2941 aaggatgtcg ggcaccgcaa ccggatcttc cataccccag gcgagcgaga ggtcaccctg
     3001 ccagcgggac acggctccat gccagcgatc ggcgtagatc gccctttcgt ccatgtacga
     3061 aacaagcttg tgaccgagcc ggttgccgtc gttccaggtc atcaggctcc agtaatccgt
     3121 cgcgtcggca tcggtcagcg gatgcgacgg gccaaagacc gaagcgaact ggcgccggaa
     3181 gaaggcctcg ttcgagaatc ttgccgcgag cgggccgagc gggccgcgga gcagcttttg
     3241 ggcgagggtc gggttcgacc tttcgaccag gatcgagccg ttgaaaagca tcattccggc
     3301 cagctcgaag cgaagctcgt gggcgaggtc cctggccatc agctcggtcg cgaccgaagc
     3361 gccgaagtcg tgggcgcaga tgaaggcggg cttctcgccc cagtggcggc agatcagttc
     3421 ctcggtcagg tcggcctgcc aggaaagcgt gtacgaatgg tcgcgcggct tgtcggagaa
     3481 tccgaaaccg aggaagtcga aggcgagcat cgcccggtcg ggcatcagtt cgaacagata
     3541 acgccagtca aaggaacagg tcgggaagcc gtgaagcagg accagcagcg ggccctcgcc
     3601 gtcctggaag caggcgtgga tctggcgccc gcggaactcc tcgagcccgc ccttgaggcg
     3661 ccatgtctcg accctttcgg aaaggctgat tgcgctgctc atgcgaccac cggctctccg
     3721 ttgcggatcc cggcgacgat ctccctcgcc cggtcgatac cggccgtctg gcagagctcg
     3781 tttcggacga gtttggggac gtcaagctgg tcgaggtacc agggatagaa cttccggagg
     3841 tagcggccgg ccctctcttc gccgaggtgt tcctcggccc gatccatgac ccaggccagt
     3901 tcgtccatga tctcctcacg cgaaggccgt tcaatttcct gtccggtcag ttcggtgaag
     3961 acccacgggt aaccgaagga accgcgggcg atcatcaccg cgtcggcgtt cgattcctcg
     4021 taggcgcggc gg
//
LOCUS       DAOTIP010000090         4031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_26405_length_4031_cov_3.724095, whole genome shotgun sequence.
ACCESSION   DAOTIP010000090 DAOTIP010000000
VERSION     DAOTIP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26405_length_4031_cov_3.724095"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..330
                     /locus_tag="P5138_02615"
     tRNA            247..330
                     /locus_tag="P5138_02615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:281..283,aa:Leu,seq:gag)
     gene            442..1062
                     /locus_tag="P5138_02620"
     CDS             442..1062
                     /locus_tag="P5138_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HRV59497.1"
                     /translation="MRRLIPLVLGLALLWAPTHSEAVTMQTTRFVAGFEGFVSCPYAD
                     PAGHATIGYGHLLHYGPPTRADRKKWGCLTKAEALKLLKKDLRQTEKEVFDRIRGAKA
                     NAPMITALTSFAFNLGAGYLDFVPRRGKRPATNIGRAMRVGKYWRAAKQMLYFDGVIV
                     GGKRYELAGLRIRRRKEYRLISRGIRQLKACDGVCKDDSGGGIGVK"
     gene            1148..2404
                     /locus_tag="P5138_02625"
     CDS             1148..2404
                     /locus_tag="P5138_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59498.1"
                     /translation="MHPVASHSIRHLIALALALLLTAGLAGTASAAGQGSITYVKDND
                     IWLTNPDGTGRFRVTWDGTADSPYRSPSQADNGTIAAGHLNDIVVMKQNGEVIRKIDP
                     PALVNSASHPMDGAPVDVAISPNARIIAWSFVGYECDPTVSCGTRTATGYTDASGRTP
                     VSKYGSTFLYDPSWVGNTRTIQSGGYGSQVMLHDIGQQPVHWFDDDDYAENSTDLSDS
                     ELSPDGRRVASVRGYGSNTQVFTYDVTGNAKTGPAPAVPSPKCGTSTEEGIAGPTWAP
                     DSDRLAWQNSEGIWTGNMSDCENAGLKLVVPGGTEPDWGPARIDPSPGGIRLPFNSLR
                     RALKKGLAIDFISIGKGRVEFTVKLGRRVIAADHGTAPNGGPLRAVLKIRRSARKRLA
                     GMKKASLTISLTQKGKTAYGKGLLRK"
     gene            complement(2405..2842)
                     /locus_tag="P5138_02630"
     CDS             complement(2405..2842)
                     /locus_tag="P5138_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59499.1"
                     /translation="MDRKVGAALAVAIIGVIVGVIGIVMAKDAKDANKDTQAALEKQI
                     QRNDRSSAAKVAAATAAAKKVENKITREAATAEKQDEANLQQSEKKSSAQVSSNAASI
                     QTLEDKIDELTNEVSTLESELNTAKSKLNERITNLNFKTSGAD"
     gene            2973..>4031
                     /locus_tag="P5138_02635"
     CDS             2973..>4031
                     /locus_tag="P5138_02635"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRV59500.1"
                     /translation="MTEDPSAAVMWFRRDLRTHDLPALAEAARADRILPCFIFDDRLL
                     TRGRFVSARRTAFMLGCLKALREDLREIGADLILRHGRPEEAIPELARESGASSVHWT
                     ADVSPFARARDSRVGSALADAGITAVPHPGAYIADRPERILTKNGRPYTVFSPFNRAW
                     QESERRPAEERPDKLRLAGEPDPGEFPNLEDLGLEPDESDRSDSFEPGEAAGRAATKR
                     FLNDGLQDYADRRDQPSGGSSRLSPWIRWGCLSPLELETKLGRRSGEGARRFRAELAW
                     RDFYASVLSNFPEVTRLEYQERYRDTLDWQQDDELLAAWKSGATGYPLVDAGLRQLKA
                     EGWMHNRVRMVVASFLTKD"
BASE COUNT          717 a         1326 c         1274 g          714 t
ORIGIN      
        1 tatttgccta tgtagtcgaa gatttcgcgt tgtctcttgg tcagatcaac cacgttgctc
       61 cttttcggct ctcggccttg ttccggaaaa cggtccgacc cggggtggcg aacctgtgtt
      121 cgtaatgtat ccggacctgc ggacgtatgt tcgtgcgggg cctgaatccg caagatgcca
      181 cccggaaagg gccccgacca gtctgcgccg cctgcctccc ttcccctaga atccgcggac
      241 cttcttgcgg ctgtggcgga attggtagac gcgcaagctt gaggggcttg tgtccgcaag
      301 gacgtggagg ttcgactcct ctcagccgca tacaaatggc gtcgcttcgc ttccgggcgc
      361 ggcggcggct tgccgggcga gccgcttcgc cctggtcccc cggcagcggc gggaccgggt
      421 tggctgctat ccattgtggc gatgcgccgc ctcattcccc tcgttctcgg ccttgcgctg
      481 ctctgggctc cgactcactc ggaggccgtg accatgcaga cgaccaggtt cgtcgcgggg
      541 ttcgagggat tcgtgtcctg cccttacgcc gacccggccg ggcacgcaac gatcggctac
      601 ggccacctgc tgcactacgg ccctccgacc agagccgacc gaaagaagtg gggatgcctg
      661 accaaggccg aggccctgaa actccttaag aaggacctgc ggcaaaccga aaaggaagtc
      721 ttcgaccgga tccgcggcgc gaaggcgaac gcaccgatga tcactgcgct cacctccttc
      781 gccttcaacc tgggcgccgg atacctcgac ttcgttcccc gccgcgggaa acggccggcg
      841 accaacatcg gccgggcaat gagggtcggc aagtactggc gggccgcgaa gcagatgctc
      901 tacttcgatg gggtgatcgt gggcggcaag cgctacgagc tcgcgggcct ccgcatcaga
      961 cgccggaagg agtaccgcct gatttcacgg ggtatcaggc agctgaaggc ctgcgacggt
     1021 gtctgcaaag acgattccgg cggcggcatc ggcgtgaagt agcggcctcc acggctgcaa
     1081 cgggtccgca agataagggc aaggtctcct tcgtagcttg ctggcaacag caccgacaag
     1141 gagacccatg caccccgttg ccagccattc cattcgccat ctcatcgccc tcgctcttgc
     1201 cctgctgctc acggccggcc tggccggcac cgcttccgct gccggccagg gttcgatcac
     1261 ctacgtgaag gacaacgaca tctggctcac caatcccgac gggactggcc ggttcagggt
     1321 cacctgggac ggaaccgcag actctcccta ccggtcgccg agccaggccg acaacgggac
     1381 gatcgcggca ggacatctca acgacatcgt ggtgatgaag cagaacggcg aggtgatacg
     1441 gaaaatcgat ccaccggcac tggtcaactc ggccagtcac ccgatggacg gggctccggt
     1501 cgacgtcgcg atctcgccca atgcccggat catcgcctgg tcattcgtcg gatacgaatg
     1561 tgacccgacc gtcagctgcg gaacccgtac cgccaccggc tacacggatg ccagcggtcg
     1621 cacgccagtc tcgaagtacg gctccacatt cctgtacgac ccatcctggg tgggcaatac
     1681 acgcaccatc cagagcggcg gctacggctc gcaggtgatg ctccacgaca tcggccagca
     1741 gccggtccac tggttcgatg acgacgacta cgccgaaaac tcgaccgatc tgagcgacag
     1801 cgagctgtcg ccggacggcc ggcgggtggc cagcgtccgc ggctacggca gcaacacgca
     1861 ggtattcact tacgacgtaa ccggcaacgc gaagacgggc ccggcacccg cagttccttc
     1921 accgaaatgc gggaccagca ccgaggaagg gatcgccgga ccgacctggg ctccggattc
     1981 ggaccggctc gcctggcaga acagcgaagg catctggacc ggaaacatga gtgattgcga
     2041 gaacgccgga ctgaagctgg tggtcccggg cggcaccgag ccggattggg gaccggcccg
     2101 gatcgatccc tcaccgggcg gaatccggct ccccttcaac agccttcgcc gggcgctgaa
     2161 gaaaggactc gccatcgact tcatctcgat cggcaagggc agggtcgaat tcacggtgaa
     2221 gctggggcgc agggtcatcg cggccgatca cggcaccgcc cccaacggcg ggccgctccg
     2281 ggcggtcctg aagatcagga gatccgcccg caaacgactt gccgggatga agaaggccag
     2341 cctgacgatc agcctcaccc agaagggcaa gaccgcctac ggaaaggggc tgctccgaaa
     2401 gtgatcagtc ggcgccgctc gtcttgaagt tgaggttggt gatccgttcg ttcagcttgc
     2461 tcttggccgt attcagttcg ctctcaagcg tggagacctc gttggtgagc tcgtcgatct
     2521 tgtcttccag cgtctgaatg ctggccgcat tggacgacac ctgggcggat gacttcttct
     2581 ccgactgctg cagattggct tcatcctgct tctccgcagt tgccgcttcc ctggtgatct
     2641 tgttctcgac cttcttcgca gcggccgtag cggcggccac ctttgcggcg gaactccggt
     2701 cgttccgttg aatctgcttc tcgagcgctg cctgggtgtc cttgttcgcg tccttggcat
     2761 ccttggccat cacgattccg atcacgccga cgatcacgcc gatgattgcg acggcgaggg
     2821 cagctccgac tttccgatcc atttgatttc ctccccgggc tgctgagcgg ccggattcag
     2881 ggttgaacga gacaccggaa ctctgccatc ccgaccggac acgcgaacta gactggcgcg
     2941 atgggcaagg ggactttgat ccggaagatg acttgacgga ggacccgtca gccgcggtca
     3001 tgtggttccg acgggatctc cggacccatg accttccggc ccttgccgag gcggccaggg
     3061 ccgaccggat actcccctgc ttcatcttcg acgaccggtt gctgacccgg gggcgattcg
     3121 tttcggcccg cagaaccgcc ttcatgctcg gctgcctgaa ggcgcttcgc gaggatctgc
     3181 gcgaaatcgg cgccgacctg atcctgcgtc acggacgccc ggaggaggcg attcccgagc
     3241 ttgcacgcga gtcgggagct tcgagcgttc actggaccgc tgacgtctcg ccgttcgccc
     3301 gggcccggga cagccgggtc gggtcggcgc tggccgacgc cggaatcacc gctgttcccc
     3361 atcccggcgc ctatatcgcc gaccggccgg agcggatcct cacgaagaac ggccgcccct
     3421 acacggtgtt cagcccgttc aacagggcct ggcaagagtc cgagcgaagg ccggccgagg
     3481 aacgcccgga caagttgaga ctggcgggag aaccggaccc gggtgaattc ccgaacctcg
     3541 aggacctcgg gctggagcct gacgaatctg accggagtga ctcctttgag cccggcgagg
     3601 ctgccgggcg agccgccacg aagcggttcc tgaacgacgg acttcaggat tacgcggatc
     3661 gtcgtgacca gccttccgga ggctcctccc ggctctcccc ctggatccgg tggggttgcc
     3721 tttcaccgct cgagctggag accaagctcg gacgccggtc cggcgaggga gcacgacggt
     3781 tccgggccga actcgcctgg cgtgatttct acgcttcggt gctttcgaac tttcccgagg
     3841 tgacccggct cgagtaccag gagcgatacc gggacaccct cgactggcag caggacgatg
     3901 aactgctggc cgcctggaaa tcgggggcta ccggctaccc gctggtcgac gccggattgc
     3961 ggcagttgaa agccgaaggc tggatgcaca accgggtccg gatggtcgtc gcctccttcc
     4021 tgaccaagga c
//
LOCUS       DAOTIP010000091         4020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_26507_length_4020_cov_3.234552, whole genome shotgun sequence.
ACCESSION   DAOTIP010000091 DAOTIP010000000
VERSION     DAOTIP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26507_length_4020_cov_3.234552"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="P5138_02640"
     CDS             complement(<1..291)
                     /locus_tag="P5138_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV59501.1"
                     /translation="MPTVAEIMDREPATVSPDDTIQRVIETLQTHDLPGVPVVDETRK
                     VLGIVTESDLVISNEDSDFHLPHYVNIMGGIVFLESTKHWEERAKKAFAATAA"
     gene            complement(307..1845)
                     /locus_tag="P5138_02645"
     CDS             complement(307..1845)
                     /locus_tag="P5138_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRV59502.1"
                     /translation="MEDWLDPVYDAAGMGAADRWAIEQVGVPSLELMEAAGRGLARAT
                     AELVSDGTGLVSGGSVTVVCGKGNNGGDGLVAARHLAESGFDVAVILFGDRDQLSPDS
                     QANLDRLPEGLAEVLEGGLSASSLKGVVIDAMLGTGFEGEPREPVAGTIRAINDSDLP
                     VVACDVPSGVNASTGEASEVVVRADRTVTFHQRKLGHLIAPGKQLCGPVEVIPIGIPE
                     GAPPPEAGGAIRARVLDLLPRRGADSNKFTSGRVSIVGGSRGLTGAVCLAAEAAIRAG
                     AGYATAAVPGGLEDIFEAKLTEVMTKGFGEGNELAPDQEKALLDHLAGSASVVLGSGI
                     GRAPGTSQLIAGLAWGLKVPTVIDADALSVIGTGLDPIASREAPTVLTPHDGEAGRLL
                     GRESAEVSAHRLASARELASRTGAVVVLKGDDTIVTDGEKVAVNTLPAPALATAGTGD
                     VLAGVVGGFLVRGLGAFEAAAAAVFAHAAAGEIASLEVGNRDGVIATDVIAAIPRAMI
                     PPAR"
     gene            complement(1845..2219)
                     /gene="acpS"
                     /locus_tag="P5138_02650"
     CDS             complement(1845..2219)
                     /gene="acpS"
                     /locus_tag="P5138_02650"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRV59503.1"
                     /translation="MADGVGMDLIEVARIERALDRHPRLADRLFTEAELEYSSSKGRP
                     GRHLAARFAAKEAVVKALELGAGTSLREIEVVAGSDERPAPTIRLSGRARERAVERGL
                     SVRVSLTHAKEMAGAVAIAGRD"
     gene            complement(2236..3870)
                     /locus_tag="P5138_02655"
     CDS             complement(2236..3870)
                     /locus_tag="P5138_02655"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HRV59504.1"
                     /translation="MPSQFRKYSVPSILAAGLAALVLSACGGSSDDSGASELAKYAPA
                     DVPVYIEGAVQPDSQVASNVDSLSEELAGVNVGDLIKDAINNSSGSDVNFDADVKPWL
                     GQDAAVFVQFDPSTLTNSVDSASGGSLSSLSSDNSGMTSYDGTDDQTYGLIVQTTDTD
                     AAQSFIDKQSKTDGGSTDGEYEGFSYKVAKDDGSAVGIVDDNVVIGSTEEGFKAAVDA
                     SKGDNLADTDAFSDLSGHVADGALASVFASNDPYIKALKEQGLDLGGLYSALGIDLEG
                     SGTVLSLVPESNEISVQGYTNAGSDLTSGDPTSLIKTFPADTVFATGSGDIGENATKI
                     VNALNDEGIPGVIEAGQVDQFINEASGQIDIKGIISSLETVAFFVNGNSEKTLGGALV
                     ATSSDIKPIESSLRGISSLIGLAGDASVRPLPGGVAGFRVFTPDLPGRPIVVGVKGDR
                     MVIGVGMKASLNALTGSGQTLADSDAFKAADDSTEAAGLDMFANPATIAKLIVGSSGG
                     DPDAKQIADVVRKFEYIAAGSSDADGTFEFNMGLQN"
BASE COUNT          643 a         1330 c         1331 g          716 t
ORIGIN      
        1 ggcggccgtg gcggcgaagg ccttcttggc ccgttcttcc cagtgcttgg tggattcgag
       61 gaagacgatg ccgcccatga tgttcacgta atgcggcagg tggaagtccg agtcctcgtt
      121 cgagatgacc aggtcggatt cggtcacgat gccgagcacc ttgcgggtct cgtcgaccac
      181 cggaaccccg gggaggtcat gagtctgcag ggtctcgata acgcgctgga tggtgtcatc
      241 cggcgagacg gtggcgggct cacggtccat gatttcggca acggtcggca tcggcggcag
      301 cttatttcat ctggcgggcg ggatcatcgc ccgggggatc gccgcgatca cgtcggttgc
      361 gatcactccg tcccggttgc cgacctcgag cgaggcgatc tctcctgccg cggcatgtgc
      421 gaagacggct gcggcggcag cttcaaaggc cccgaggccg cggaccagga acccgcccac
      481 gacgcccgcc aggacgtcgc cggtcccggc ggtcgcgagg gccggtgccg gaagcgtgtt
      541 caccgccacc ttctcgccgt cggtgacgat cgtgtcgtcg cccttcaata cgacgacggc
      601 cccggttcgg ctcgcgagct cgcgggccga ggcaagcctg tgggccgaga cctcggccga
      661 ttccctgccg agcaacctgc ccgcctctcc gtcatgcggg gtgagcacgg tcggtgcctc
      721 gcggctggcg atcggatcca gaccggttcc gatcaccgac agggcgtcgg cgtcgatcac
      781 ggtcgggacc ttgagtcccc aggcgaggcc ggcgatcagc tgggaggttc ccggcgcgcg
      841 ccctataccc gaaccgagca ccacggaggc cgaaccggcc agatggtcga gcagggcctt
      901 ctcctgatcc ggagccagct cgttgccctc gccgaaaccc ttcgtcatga cctcggtcag
      961 tttcgcctcg aagatgtctt ccaggccgcc gggcacggcg gccgtcgcgt atccggcgcc
     1021 ggcccggatc gccgcctcgg cggccaggca gaccgcgccg gtcaggccac gggatccgcc
     1081 cacgatcgag acccggccgg aggtgaactt gttcgagtcc gccccccgac ggggcagcag
     1141 gtcgagcacc cgcgcccgga tcgccccgcc ggcttcgggc ggtggcgccc cttccgggat
     1201 gccgatcggg atcacctcta cgggcccgca aagctgtttg cccggggcga tcagatgccc
     1261 gagctttcgc tggtggaagg tgacagtccg gtcggcccgg accacgactt ccgaggcttc
     1321 cccggtcgag gcgttcactc ccgagggcac gtcacaggcg acgaccggaa gatcggagtc
     1381 gttgatcgcc cggatcgtcc cggccaccgg ttcgcggggc tccccctcga atccggtccc
     1441 cagcatggcg tcgatcacca ctcccttcag agaagaggcg gaaagcccgc cttcgagcac
     1501 ctcggccaga ccttccggca ggcggtccag gttggcctgc gagtcgggac tcaactggtc
     1561 gcggtccccg aagaggatca ctgcgacgtc gaacccggat tcggcgaggt gacgggccgc
     1621 taccagaccg tcaccgccgt tgttgccctt tccgcagacc acggtcacgc tgccgcctga
     1681 caccagcccg gtgccgtcgg acacgagttc cgcggtggcc cgcgccaggc cccggcccgc
     1741 cgcttccatc aactcgagtg aaggcacgcc gacctgctcg atcgcccagc ggtcggccgc
     1801 gcccattcca gccgcgtcat agaccgggtc gagccagtcc tccatcagtc tcttccggcg
     1861 attgccaccg caccggccat ctctttggcg tgggtcaggc tgacccggac cgagagtccg
     1921 cgttcaactg cccgctcccg cgccctgccc gagagccgga tggtcggggc gggccgttca
     1981 tcgcttccgg ccaccacttc gatctcccgc aggctggttc cggcacccag ctccaacgct
     2041 ttcaccacgg cttccttcgc ggcgaagcgg gccgccagat gccggcccgg acgccctttc
     2101 gaggacgagt attcgagttc ggcctcggtg aaaagccggt cggcgaggcg gggatggcga
     2161 tcgagtgccc gctcgatgcg cgccacctcg atcagatcca tccctacccc gtcggccatt
     2221 gcttgcgcgc gcctcctagt tctggaggcc catgttgaat tcgaaggtgc cgtccgcgtc
     2281 gctcgaaccg gccgcgatgt actcgaactt gcgaaccacg tctgcgatct gcttcgcatc
     2341 cgggtcgccg ccgctcgagc cgacaatcag cttggcgatg gtggccgggt tcgcgaacat
     2401 gtcgagtccg gcagcttcgg tcgagtcatc cgccgccttg aaggcgtcgc tgtcggcaag
     2461 ggtctggccg cttccggtca gcgcgttgag cgaggccttc atgccgacgc cgatcaccat
     2521 gcggtcgccc ttcacaccga ccacgatcgg gcggcccggc agatccggcg tgaagacgcg
     2581 gaaaccggcg acgccacccg gcaggggccg aacgctggcg tcaccggcga gcccgatcag
     2641 cgaggagatg ccgcgaagcg agctctcgat cggcttgatg tcggagctgg tggcgaccag
     2701 ggcgccaccc agggtctttt cggagttgcc gttgacgaag aaggcaaccg tctcgagcga
     2761 actgatgatt cccttgatgt cgatctggcc ggaggcctcg ttgatgaatt gatcgacctg
     2821 tccggcctcg atgactcccg ggatgccttc atcgttcagg gcgttcacga tcttcgtggc
     2881 gttttcgccg atgtcaccgg aaccggtcgc gaagacggtg tcggcgggga aggtctttat
     2941 cagcgaagtg gggtcaccgc tggtcaggtc cgaaccggcg ttggtgtaac cctgaaccga
     3001 gatctcgttt gattcgggaa ccaggctgag cactgttccg cttccctcga gatcgattcc
     3061 gagcgcggag tacaggccgc caaggtcaag accttgctcc ttgagagcct tgatgtacgg
     3121 atcgttggag gcgaagacgg aggccagggc accgtcggca acgtgacccg agagatccga
     3181 gaaggcatcg gtatcggcaa ggttgtcgcc cttcgaggcg tcgactgcgg ccttgaaacc
     3241 ttcttccgtc gagccgatca ccacgttgtc gtcgacaatt ccgacggccg agccgtcgtc
     3301 cttggcgacc ttgtacgaga agccttcgta ttcaccgtcg gtcgatccgc catcggtctt
     3361 cgactgcttg tcgatgaagg actgcgcggc atcggtatcg gtggtctgga cgatcaagcc
     3421 ataggtctgg tcgtcggtcc cgtcgtagct ggtcattccg gagttgtcgg agctcagcga
     3481 agacaggctg ccgccggaag ccgaatcgac cgagttggtc agggtcgagg ggtcgaactg
     3541 gacgaagacc gcggcgtcct gtccgagcca gggcttgacg tccgcgtcga agttgacgtc
     3601 gctgccactg gagttgttga tggcgtcctt gatcaggtcg ccgacgttca cgccggcaag
     3661 ctcttcgctg agcgaatcga cattggatgc gacctggctg tccggctgga cggcaccttc
     3721 gatgtagacg ggtacgtcgg ccggcgcgta cttggcgagc tctgacgcac ctgagtcatc
     3781 ggagctgcct ccgcaggcgc taagcacgag ggccgcgaga ccggcggcaa gaatggacgg
     3841 gactgagtac ttcctgaatt gactgggcat ggccgaaatc ctaacgccga cccttgcgaa
     3901 attcttgcga atcgaacaga ggggttactc gaccgtgacg gtcttggcga gatttctcgg
     3961 ctgatccacg ttgaggccgt tgaggcgggc cacgtggtag gcgagcagct gcagcggaag
//
LOCUS       DAOTIP010000092         4013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_26572_length_4013_cov_2.853714, whole genome shotgun sequence.
ACCESSION   DAOTIP010000092 DAOTIP010000000
VERSION     DAOTIP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26572_length_4013_cov_2.853714"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="P5138_02660"
     CDS             complement(<1..835)
                     /locus_tag="P5138_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRV59505.1"
                     /translation="MAKRATKRGDERTDLSGSRDVLICGASFAGLMAARQLAGSGADV
                     LVIDRYEIGERQTSACAIPTAWLEELGLIESEKQRFDSVLIHTAHGSIRLPVPWSFST
                     FDYPTFCEMLWEQCDAEFETAKVNGKADADGRAEVSRRAETNGDGAHHIGTDRGIISS
                     PLVVDSLGWKRVLAGGAHYQPPDAPLSRGLEVHPPGGNVDLEVFINRRYVPAGYGWSF
                     PADDEVRVGVGSFDPYFPVRPTTDLLADDIEVGKSAYQGNWIPHKLRPATADGIFFAG
                     DS"
     gene            993..2522
                     /gene="rpsA"
                     /locus_tag="P5138_02665"
     CDS             993..2522
                     /gene="rpsA"
                     /locus_tag="P5138_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRV59506.1"
                     /translation="MTTTETTLQPAPEGVPVEGSNGLLINVDGRIIPNYAATIVSFEE
                     GDVVNGKVVRIDRDEVLLDIGYKSEGVIPASELSIRKNVDTSEEVELGEEIDALVVTK
                     EDSEGRLILSKKRARFEKAWRKIEAAAESGQPVEGNVIEVVKGGLILDLGIRGFLPAS
                     LVDIRRVHNLDEFMSQTLECKVIELNRSRNNVVLSRRAVLEEERKEVRDQILDRLQPG
                     EVVEGKISNIVDFGAFVDLEGIDGLIHISELSWSHVNHPSEVVAIGDTVKIKVLDIDR
                     DRQRISLGLKQTQEDPWQRVVSTYRSGDVLEGTVTKVVSFGAFVEILDGVEGLVHISE
                     LADHHVENPSEVVELGASLNVKILEIDEERRRLSLSIKRVEGQNMLLKDLGAALAAAS
                     GEPAAEAEAPELGLSEEVFTEETGAEAPVDAADEVAPEVEAVVEEAIHEAEVADAAAE
                     VAGEDETPEEVVEDAVAEVEIEAAADEAAAEEAVVEEAEEITEEAAEAEGEAPEGEEQ
                     S"
     gene            2531..3115
                     /gene="coaE"
                     /locus_tag="P5138_02670"
     CDS             2531..3115
                     /gene="coaE"
                     /locus_tag="P5138_02670"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRV59507.1"
                     /translation="MTGGVAAGKSEALSAFGRLGAATISADQVVHDLLDQEPLLGRLR
                     ERWGDEVAPEGRVDRSVVGAKVFNDPEELAWLESQIHPLVGEEFRGWLEGLGPETKFA
                     VAEIPLLFEGEMYRAFDHTVAIVAHEEIRQDRARSRGHEGVEGREARQLSQAEKAGRA
                     DTVIENDGTPEELEAKLAALLDSFESELATGGQG"
     gene            3134..3775
                     /locus_tag="P5138_02675"
     CDS             3134..3775
                     /locus_tag="P5138_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRV59508.1"
                     /translation="MAPNRTSTAGGRRSRQAPRRSNSKPRTGAGRAFLILAGLIAIGI
                     IAILIISGLASKGIKEVALPLKHEDVIRQQSQEKGVDAALIAAVIYAESQFVDQTSHA
                     DARGLMQITPETAEEIEKLSGGTTFKLEDLRDPELNIRYGTYYLKHLLDMYDGDVVAA
                     LAAYNAGPGNADTWGGAGMTIDDITFPETRAYVEKVLEKQREYKHEYALELGY"
     gene            3775..>4013
                     /locus_tag="P5138_02680"
     CDS             3775..>4013
                     /locus_tag="P5138_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59509.1"
                     /translation="MNLRTAALAGAVGLVLADSSIVVLALPEIFRQFDTTISGVSWVL
                     IIFNLVLALLAVPAAHLARRFGPGRSAAVGLAVFAG"
BASE COUNT          748 a         1275 c         1337 g          653 t
ORIGIN      
        1 ccgagtcgcc ggcgaagaag atgccgtccg cggtggcagg acgcagcttg tgggggatcc
       61 agttgccctg gtaggccgat tttccgacct cgatgtcatc ggcgagcagg tcggtggtgg
      121 ggcggaccgg gaaatacggg tcgaaagagc cgaccccgac ccggacctcg tcgtcggccg
      181 ggaatgacca gccgtagccg gccggcacat agcgccggtt gatgaagacc tccaggtcca
      241 cattgccacc gggtgggtgt acttcgagac cacgtgagag cggggcatcc ggcggctggt
      301 agtgggcacc gcctgcaagg acccgcttcc agccgagaga gtcgaccacc aggggcgagg
      361 aaatgatccc gcgatcggtg ccgatgtggt gggcgccgtc gccattggtt tcggctctgc
      421 ggctgacttc ggctctccca tcggcatcgg ctttgccatt gaccttggcc gtttcgaatt
      481 ccgcgtcgca ctgttcccag agcatctcgc agaaggtcgg gtagtcgaag gtggagaacg
      541 accagggcac gggcagacgg atcgaaccgt gggcggtgtg gatcaggacc gagtcgaatc
      601 gctgcttctc ggactcgatc aggcccagct cctcgagcca ggcggtcggg atcgcgcagg
      661 cggaggtctg gcgctcgccg atctcgtagc ggtcgatgac gagaacatcg gctcccgaac
      721 cggccagctg ccgggcagcc atcaggccgg cgaagctggc tccacaaatg agaacgtcgc
      781 gggaaccgct cagatccgtc cgctcgtcac cacgctttgt cgcccgtttc gccattcgca
      841 gcagggtacc cgaggcgaac ctgcccgcga atgccgccga ccttgggaag tggggcggtt
      901 tacccgtcca tgtgacgtac aaagcaaccc actcctggcc ttgaggggac tgggacgcgg
      961 gttcggcttc gttctgccat catccccgga ctgtgactac tactgaaacc acactccaac
     1021 ccgccccaga gggggttccg gtcgaaggct caaacggcct gctgatcaat gttgacggcc
     1081 ggatcattcc taactacgca gcgaccatcg tctccttcga ggaaggcgac gtcgtcaacg
     1141 gcaaggtcgt ccgcatcgac cgtgacgaag tgctgctcga catcggctac aagtccgaag
     1201 gcgtaatccc cgcctccgag ctctcgatcc gcaagaacgt cgacacctcc gaagaggtcg
     1261 agctcggcga agagatcgac gccctcgtcg tcaccaagga agactccgaa ggccgcctga
     1321 tcctctccaa gaagcgtgcc cgcttcgaga aggcctggcg caagatcgaa gctgccgccg
     1381 aatccggcca gccggtcgaa ggcaacgtga tcgaggtcgt caagggcggc ctgatcctcg
     1441 acctcggcat ccgcggattc ctgcccgcct ccctggtcga catccgccgt gtccacaacc
     1501 tggacgagtt catgtcacag acgctcgaat gcaaggtcat cgagctcaac cgcagccgca
     1561 acaacgtcgt gctttcccgc cgcgccgtcc tcgaagagga gcgcaaggaa gtccgcgacc
     1621 agatcctgga tcgcctgcag ccgggcgagg tcgtcgaggg caagatctcg aacatcgtcg
     1681 atttcggcgc gttcgtggac ctcgagggca tcgacggcct gatccacatc tccgagcttt
     1741 cctggagcca cgtcaaccac ccgtccgagg tggtcgcgat cggcgacacg gtcaagatca
     1801 aggtgctcga catcgaccgc gaccgccagc gcatctcgct gggtctcaag cagacccagg
     1861 aagatccgtg gcagcgcgtg gtttcgacct accgctccgg cgacgtgctc gagggcacgg
     1921 tcaccaaggt cgtttccttc ggtgccttcg tcgagatcct cgacggggtc gagggcctgg
     1981 tccacatttc ggagctggct gatcatcatg tcgagaaccc gtcagaggtc gtcgagctcg
     2041 gtgccagcct caacgtcaag atcctcgaga tcgacgagga gcgccgtcgc ctttcgctct
     2101 cgatcaagcg ggtcgaaggt cagaacatgc ttctgaagga cctgggggca gcccttgcgg
     2161 ccgcttccgg cgagcccgcc gccgaggcgg aggctcccga gctcggtctc tccgaggaag
     2221 tcttcaccga agagactgga gccgaggccc cggtcgacgc cgccgacgaa gtggcccccg
     2281 aggtcgaggc tgtggtcgag gaagcgattc acgaggccga agtggccgac gccgccgcgg
     2341 aagtcgccgg cgaggacgag actcccgaag aggtcgtcga agatgccgtc gccgaggtcg
     2401 agatcgaagc cgctgccgat gaagccgccg ccgaggaggc tgtggtcgag gaagccgaag
     2461 agatcaccga agaggccgct gaggccgagg gtgaggctcc cgaaggggaa gaacagtcct
     2521 gacgatcggg ctgaccgggg gcgtagctgc cggtaagtcc gaggcactga gcgcctttgg
     2581 gcgactcggc gcggcaacaa tctctgccga ccaggtcgtc cacgacctgc tcgatcagga
     2641 gccgctgctg ggtcgcctca gggagcgctg gggtgatgag gttgccccgg aaggccgggt
     2701 cgaccgcagt gtcgtgggcg cgaaggtctt caacgacccg gaggaactgg cctggctcga
     2761 atcccagatc cacccgctgg tcggagagga attccgcggc tggctcgaag gcctcggacc
     2821 ggagacgaaa ttcgcggtgg ccgagattcc cctgctcttc gaaggggaga tgtaccgcgc
     2881 gttcgatcac acggtggcga tcgtcgccca tgaggaaatc agacaggacc gggcccgttc
     2941 gcgcggtcat gagggagtcg aggggcggga ggcccgccag ctgagtcagg ccgagaaggc
     3001 cggccgggcc gacacggtga tcgaaaacga cggcacgccg gaagaactcg aggccaagct
     3061 cgcggccctg ctcgattcct tcgagtcaga gttggcgacc ggcggtcagg gctaggctgt
     3121 aaggccacct cgcatggccc cgaaccgaac ttcaactgcg ggcggtcgtc gcagccgtca
     3181 ggcgcccaga cgcagcaaca gcaagccccg caccggcgcg ggccgggctt tcctgatcct
     3241 ggccgggctg atcgcgatcg gcatcatcgc catcctgatc atctcgggac tggccagcaa
     3301 ggggatcaag gaagtcgcgc tgccgctcaa gcacgaggac gtgatccgtc agcagtcgca
     3361 ggagaaaggg gttgacgccg ccctgatcgc agcggtgatt tacgccgaat cgcagttcgt
     3421 cgaccagact tcccatgccg atgcccgggg cctcatgcag atcaccccgg agacggcgga
     3481 ggaaatcgag aagctgagcg ggggtacgac cttcaagcta gaagacctgc gggatccgga
     3541 actgaacatc cgctacggga cctactacct caagcatctg ctcgatatgt acgacggcga
     3601 cgtggtggcg gcgctggccg cgtacaacgc cggtccgggc aacgccgaca cctggggtgg
     3661 cgccggcatg acgattgacg acatcacctt cccggagacc cgtgcctacg tggagaaggt
     3721 cctcgagaag cagagggagt acaagcacga gtacgccctg gaactggggt actgatgaac
     3781 ctgcggaccg ccgcacttgc cggtgcggtc gggctggttc tcgctgattc ctcgatcgtc
     3841 gtgctggccc tgccggagat cttccgtcag ttcgacacga ccatctccgg ggtctcctgg
     3901 gtgctgatca tcttcaacct ggtgctggcc ctgctggcgg taccggcggc ccacctggcc
     3961 cgtcgcttcg gccccggtcg ttcggcggcg gtcggactcg ccgtgtttgc cgg
//
LOCUS       DAOTIP010000093         3976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_26902_length_3976_cov_4.018108, whole genome shotgun sequence.
ACCESSION   DAOTIP010000093 DAOTIP010000000
VERSION     DAOTIP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26902_length_3976_cov_4.018108"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="P5138_02685"
     CDS             complement(<1..1296)
                     /locus_tag="P5138_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59510.1"
                     /translation="MAVATMVSPVEAAKDDTTLLSLAKVIPGGKVNADVDDFSISLSA
                     NGRFMAFTTNATNLDPNDTNSDPDIYVRDTLLDTTTLVTPGDGFDDAAGNEPSISDDG
                     QKVAFVAFDDIDPDDNNGENDVYVADLSTPAPTLELVSRPTGTGAAAPAVPEGVLFAN
                     PEISGDGSAVAFGSLDNSLDPTNDSDTLLGYFYSGDIFVRDLDTDTTELVSRNATVDG
                     NRASYNPSIDTDGSHVSFDTQSSNLDPGDSDEERDVYVRDRTGNTLTLASRADGAAGA
                     KANDLAVGSSLSGDGQAVSYVSAATNLDAADSDATLDVYLRNIVGDDTELISRADGAA
                     GDKGNKASQNPSLSADGNRVAFISAAHNLDPAMTTPGNTNFAYVRDLNTNTTRLQSRA
                     DGGSGAIANDGVRSITMSAGGVAIGFASKSTNLDPGDTDT"
     gene            complement(1590..1718)
                     /locus_tag="P5138_02690"
     CDS             complement(1590..1718)
                     /locus_tag="P5138_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59511.1"
                     /translation="MFDRFRIEGTGTIDPDTGKVHIKRSPRPARSTYPMNGIVATF"
     gene            1899..2597
                     /locus_tag="P5138_02695"
     CDS             1899..2597
                     /locus_tag="P5138_02695"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HRV59512.1"
                     /translation="MAKVLLLAAVSAFYPTLVALAILMLARPKPVKLFSGFLIGGFVV
                     SLAAGWAILAFADTGSIGSGSTGSTRPILSLILGLLLIGVALTLLAGHELPGAERRRR
                     SRAKKEALAEKGEKKESRVARAVARDSFWLAMVVGAALSVPSVWYLSALAEIDANQYS
                     TLVDILLVLLFNVIMFALIEISLAVCYFAPERAAADVARFDAWTHSHMREIGIAVALA
                     GGLYLAVKALLTLL"
     gene            2747..>3976
                     /locus_tag="P5138_02700"
     CDS             2747..>3976
                     /locus_tag="P5138_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59513.1"
                     /translation="MSIRTEPAIRTARGEVRTFLATATFLWVSALFLWLPLDSKAAPQ
                     GPWKLPATTLTPPDSQALDPAVAVAADGTTSVVWQRISSPSVSVIEVASRPPGGSFGP
                     PLQVSSPGVYSEHPAIGVAPDGSTVVAWVRDNGLLDVVESASRVPGAVFFSSPEEISG
                     SSADVEPPFIAAVDSVDVVVWSQFVSGKYVVQQAGRPRNGSFSAPQDLSIPDQDAKDP
                     QAVIAPDGTTTIAWHRFDGADRIIQERTRPPGGAFAPANNLSLAGADSEAPRLGVDGD
                     GAVTAAWLRGPSGSGTVQVASRPAGQQFFGNGVDVSQAGGVSELSLSVSKSAGEALAW
                     VRNDVPVSFIIQSSGRTGTGPFPAPVDLTPSGQQAISPSVVTAPDGTVTVAWAGPAGI
                     RSATRTATGSFGPVTTLS"
BASE COUNT          594 a         1258 c         1344 g          780 t
ORIGIN      
        1 cgtgtcggtg tcgccggggt ccagattggt cgacttgctg gcgaatccga tcgccacgcc
       61 gccagcgctc atcgtgatcg accgtactcc gtcattggcg atcgcgccgc tgccgccgtc
      121 ggcccgcgac tgaagcctcg ttgtattcgt gttcaggtcc ctcacatacg cgaagttggt
      181 attccccggg gtcgtcatcg ccgggtcgag gttgtgtgcc gcactgatga atgcgacccg
      241 gttgccgtca gcgcttagcg acgggttctg ggaggccttg ttccctttgt ctccggctgc
      301 cccgtctgca cggctgatca gctcggtgtc gtcgccgacg atgttcctca ggtagacatc
      361 tagcgtggcg tcgctgtcgg cggcatcgag attggtcgcc gcgctcacat aggagaccgc
      421 ctggccgtcg ccgctgagcg acgaaccgac cgcgaggtcg ttcgccttgg ccccggccgc
      481 cccgtctgcc cggctggcaa gggtcagtgt gttgccggtt cggtcgcgga cgtaaacgtc
      541 cctttcctcg tcggagtcac cggggtcgag gttcgacgac tgggtgtcga agctgacgtg
      601 gctgccgtcg gtgtcgatcg aagggttgta cgaggcccgg tttccgtcca cagttgcgtt
      661 gcggctcacc agctccgtgg tgtcggtgtc caggtcgcgg acgaatatgt cgccggagta
      721 gaagtagccg agcagggtgt cggagtcgtt ggtgggatcg agtgagttgt ccagagagcc
      781 gaaggcgacg gccgagccgt cgccagagat ctccgggttc gcgaacagga cgccctccgg
      841 aaccgcaggt gcagcggctc ctgtgccggt cggccggctg accagctcga gtgtcggcgc
      901 gggggtcgaa aggtccgcca cgtagacgtc gttctcgccg ttgttgtcgt ccggatcgat
      961 gtcgtcgaag gcgacgaagg cgaccttctg cccgtcatcg gagatcgacg gttcgttgcc
     1021 ggccgcatcg tcgaatccgt ccccgggggt cacgagcgtc gtcgtgtcca gaagcgtgtc
     1081 gcgcacgtag atgtcggggt cggagttcgt atcgttgggg tcgaggttcg tcgcattcgt
     1141 cgtgaaggcc atgaagcgcc cgttggccga gagcgagatc gagaagtcgt ccacgtccgc
     1201 gttgaccttg cctcccggaa tcaccttggc gaggctgagc agagtcgtgt cgtccttcgc
     1261 ggcttcgacc gggctgacca tcgtggcgac ggccaacgga agcgaggcgg cgatggccgc
     1321 taacgcgatc ttgaggcttc tccctgcgac tcgtggctcc ccggccatgg tccgaccata
     1381 acacagcccc gttcggccct cggcggttca gcccatcatc acgtcgatgt ctggcaccgc
     1441 tttcgatcgt gcccttcagg tccggccgga gcgtccagca atctcggtac agctgctggc
     1501 ggaaatgtcc cgagattgaa ggaaggtgcg gagcagcgcg gggtccaggg ttcggtcgcg
     1561 accggtgtgc tgcccgggtc gagtcaccgt tagaacgtgg cgacgatccc gttcatcggg
     1621 taggtgctcc ttgccgggcg gggtgagcgt ttgatgtgga ccttgcccgt gtcggggtcg
     1681 atcgtcccgg tgccttcgat ccggaaccgg tcgaacacga agggaacatc acgggagaac
     1741 gggttggacg ttgaccgaag actgaaatga aggtgggggc cggtgctgtt gccgctgttg
     1801 ccgagcaggc tgacgacctg cccgcgccgc aggcgctgac cctttcaccc ggatcgattg
     1861 aggccccggg aaagcgccag ggctagggtt ccccttcgat ggcgaaggtt cttctgctcg
     1921 cggccgtttc ggcgttctat ccaacccttg tcgcgctggc gatcctgatg ctggcccggc
     1981 ccaagccggt gaagctcttc tcgggcttcc ttatcggcgg ctttgtcgtc agtcttgctg
     2041 ccgggtgggc gattcttgcc ttcgcggaca ccggctcaat cggttccggg tccacgggct
     2101 cgacccgacc gatactcagt ctgatcctcg gtcttctgct gatcggggtc gccctgacgc
     2161 tgcttgcggg ccatgaactg ccgggggcgg agcgccgtcg gaggtcaaga gcgaagaagg
     2221 aagcattggc tgaaaagggg gagaagaagg aatcgcgggt agctcgggcg gtcgcccggg
     2281 actcattctg gctcgcgatg gtcgtcgggg cggcgctgag cgtgccgagc gtctggtacc
     2341 tgtcggcgct cgccgagatc gacgctaacc agtactcgac tctcgtcgac atcctgctcg
     2401 tcctgctctt caacgtgatc atgttcgccc tgatcgagat ctccctggcc gtctgttatt
     2461 tcgcgcccga gcgggccgcg gccgacgttg cccgcttcga cgcctggacc cactcgcaca
     2521 tgcgggagat cgggatagcc gttgcccttg ccggcggtct ctacctcgcg gtcaaggccc
     2581 ttctcaccct gctttgagtg aaggtccccg ggcgggatcg gaacccgatc gggttgaggg
     2641 ccgtttgata ctttcggttt tcccggggac gggagacgag gaccactcgc aaattatgtt
     2701 cgagacggtt ggcaaaggga aaacccggaa aggggcagga gggcagatga gcatccgaac
     2761 ggagccggcg attcgcacgg ccaggggcga agtacgaact ttcctggcga cggcgacatt
     2821 cctctgggtt tcggcgcttt ttctttggtt gccactcgat tccaaagcgg caccgcaggg
     2881 tccctggaag ctgccggcga caacgcttac gccacccgac agccaggccc tcgatccggc
     2941 ggtcgcggtc gctgcggatg gaaccacgag cgtggtctgg caacgcattt caagcccgag
     3001 cgtttcggtg atcgaggtgg cctcgcgccc gcccggaggt tcgttcggtc cgccacttca
     3061 ggtttcatca ccgggcgtct actccgaaca cccggcgatc ggtgtcgcgc ccgacggcag
     3121 cacggtcgtg gcctgggtgc gcgacaacgg tctgctggat gtggtggagt cggccagcag
     3181 agttccaggc gccgtcttct tctccagccc ggaggaaatc tccgggagct cggccgatgt
     3241 cgaaccgcct ttcattgctg ccgtcgattc cgtggacgtc gtggtctgga gccagttcgt
     3301 ttcaggcaag tacgtcgttc agcaggccgg ccggccccgc aacggcagct tttcggcccc
     3361 gcaggacctc tcgattcccg atcaggacgc gaaggatccg caggccgtca tcgcgcccga
     3421 tggaacgacc acgatcgcgt ggcaccgctt tgacggcgcc gacaggatca tccaggagcg
     3481 gaccaggccc cccggcgggg cctttgcgcc agccaacaac ctttccctgg cgggagcaga
     3541 ttccgaagcc ccgcggctcg gggtcgacgg ggacggtgcc gtgaccgccg cctggctgcg
     3601 cggaccatcc ggttcgggga cggttcaggt cgcatcaagg cccgccggcc agcagttctt
     3661 cggcaatggg gtggacgtct cgcaggcagg cggggtgtcc gaactgtcgc tctcggtctc
     3721 gaagtcagcc ggtgaggcac tggcctgggt tcgcaacgac gtccccgtca gcttcatcat
     3781 ccagtcttcc gggcgcaccg gaacggggcc tttcccggcc ccggttgatc tcacgccatc
     3841 cggccagcag gccatctctc cgtcggttgt caccgcaccc gacggaaccg tcaccgttgc
     3901 ctgggccgga ccggccggaa tccggtcggc aacccgcacc gccaccggat cattcggacc
     3961 ggtgacgacc ctctcc
//
LOCUS       DAOTIP010000094         3957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_27075_length_3957_cov_3.910046, whole genome shotgun sequence.
ACCESSION   DAOTIP010000094 DAOTIP010000000
VERSION     DAOTIP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27075_length_3957_cov_3.910046"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="P5138_02705"
     CDS             <1..675
                     /locus_tag="P5138_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRV59514.1"
                     /translation="FFFGAYKYPRELNWVIGVVLLILTTVMGLTGYLLPFDQRSFWAT
                     IVANNITASGPIVGPYLADFLRAGPDFSATTLSRFYALHMMVVPGLIAALIGAHLFFV
                     VKLGTTAPPWVRAGRPKGSMPEEEVTAGVAREDFEDVPVNGAVAAAEIEAEPPVEEAD
                     AQAAEAEEVEAKEAEETEAAVAPEEPAEEAAAEEAAEAPAEEAPAEETDAPDDSDTSG
                     EGEVTP"
     gene            672..1385
                     /locus_tag="P5138_02710"
     CDS             672..1385
                     /locus_tag="P5138_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRV59515.1"
                     /translation="MNAREKEEYLREYEILKKKGKPFFPYAIMKDSVMMLVVAGVLIV
                     MSIVMGAEQGPKADPTTTTYTPRPEWYFFYLFELLRVIKPPALVPVATIGIPTICMVL
                     LLLLPFYDRGPERVPWKRPIATITGIMVIIAMAYLTFLGATAGSPTEIELETAPQYEK
                     GKAIVAESGCLACHSIGENGAHGGLGPDLTDIGSKIPKEAIKQSVTIGPGIMPSFQGL
                     GEQNLNAVADYLASLDGEE"
     gene            1430..2104
                     /gene="ubiE"
                     /locus_tag="P5138_02715"
     CDS             1430..2104
                     /gene="ubiE"
                     /locus_tag="P5138_02715"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRV59516.1"
                     /translation="MNSMFDRISGVYDRMNRVMTAGLDQSWRARAAERARLEPGMKAL
                     DLCCGTGDLALMLAERVGPEGEVTGADFSRPMLDLALEKAKQEGLRQAQFKWADALDL
                     PFEDESFDAVTIAFGARNLADLDKGISEMRRVLVPGGRLVILEITQPRRQPLAGFFGL
                     WFDRIVPMLGKVAGDSSAYTYLPESVRSFPDAETLAGRLDAAGLRHVRWTLMAGGIIA
                     LHSGVR"
     gene            2101..3099
                     /locus_tag="P5138_02720"
     CDS             2101..3099
                     /locus_tag="P5138_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRV59517.1"
                     /translation="MSGADEEASRRRSLPEPVTAVTDTAGDWLPARMAEVEAGLLAAV
                     EGETELSGDAVRTLNAGGKRMRPMLVLLCAGPEAGDAAVRAGIAVELVHMASLVHDDV
                     LDRAPLRRGLPTVYASAGRERATSLGDSLFSTAFRILVDGGDADAVTEVSDTAVDLAR
                     GELKQREGAFDMELTEADYFERCGLKTGRLFEAACVLGRASTGGEPGPVGLFGHRIGI
                     AFQLLDDVLDIAGPPERTGKARGTDLLDGTVTLPLIIAAGFDPALAALDLRSLNEETA
                     AVACDRIAATGALDQVRTRAIGLIDEAKAGLAGAGLEDQQRELLELIADGVVMRYS"
     gene            3161..3724
                     /gene="dcd"
                     /locus_tag="P5138_02725"
     CDS             3161..3724
                     /gene="dcd"
                     /locus_tag="P5138_02725"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRV59518.1"
                     /translation="MVLSDKTLREEIEAGRIVIDPFEERLIQPSSIDLRVDHSYRVFN
                     NSRYPFIDVKQPMEDLTELVKPADEEPFILHPGEFVLGQTLERVTVPDDMVARLEGKS
                     SLGRLGLLIHSTAGFVDAGFSGNLTLELSNVANLPITIYKGMPIGQLSFMRMDRPVDA
                     AYGSTDKGSKYQGQRGPTPSKAYLNFR"
BASE COUNT          683 a         1324 c         1296 g          654 t
ORIGIN      
        1 ttcttcttcg gcgcctacaa gtacccgcgc gagctgaact gggtgatcgg cgtggtgctg
       61 ctgatcctga ccacggtcat gggccttacc ggctacctgc tgcccttcga ccagcgttcc
      121 ttctgggcaa ccatcgtcgc caacaacatc accgcgtcgg gcccgatcgt cggcccctac
      181 ctggctgact tcctgagggc cggaccggac ttcagcgcga ccacgctttc gcgcttctat
      241 gcactccaca tgatggtcgt gccagggctg atcgctgccc tgatcggcgc ccatctcttc
      301 ttcgtggtca agctcggcac caccgccccg ccgtgggtcc gggccggccg ccccaagggc
      361 tcgatgcccg aggaggaagt gaccgcgggt gtggcccgtg aggatttcga ggatgtacct
      421 gtgaacggcg ccgtcgcggc tgccgagatc gaggccgagc cgccggtcga ggaagccgac
      481 gcccaggccg cagaagccga agaagtcgag gcgaaggaag ccgaagagac cgaagccgcc
      541 gtcgcccccg aggaacctgc cgaggaggca gctgccgaag aggccgccga ggccccggcc
      601 gaggaagcgc cggccgaaga gactgacgct cccgatgact cagatacttc cggcgaaggg
      661 gaggtgactc cgtgaacgcc cgtgagaagg aggaatacct ccgcgagtac gagatcctga
      721 agaagaaggg caagcctttc ttcccctacg cgatcatgaa ggactcggtg atgatgctgg
      781 tcgtcgccgg tgtcctgatc gtcatgtcga tcgtcatggg tgccgagcag gggcccaagg
      841 ccgatccgac caccaccact tacacgccgc gccccgagtg gtacttcttc tacctcttcg
      901 aactgctgcg cgtgatcaag ccgccggccc tggtcccggt cgccacgatc ggcatcccga
      961 ccatctgcat ggtcctgttg ctcctgcttc cgttctatga ccgcggtccc gaacgggtgc
     1021 cgtggaaacg cccgatcgcg accatcaccg gaatcatggt gatcatcgcc atggcgtacc
     1081 tgactttcct cggcgccacc gcaggttcgc cgaccgagat cgaactggaa accgctccgc
     1141 aatatgagaa gggcaaggcg atcgtcgccg agtcgggctg tcttgcctgt cactcgatcg
     1201 gcgagaacgg ggcccacggc ggcctcggcc cggacctgac cgacatcggc tcgaagatcc
     1261 cgaaggaagc gatcaagcag tcggtcacga tcggcccggg catcatgccc tcgttccagg
     1321 gtctgggtga acagaacctg aacgcagtgg ccgattacct cgcctcgctc gacggcgagg
     1381 aatgaactcg gcacctcccc gtcgaggtga tcaggagttc gccggtgagg tgaactccat
     1441 gttcgaccgg atcagcggcg tctatgaccg catgaaccgg gtgatgaccg ccggcctcga
     1501 ccagagctgg cgcgcccgtg ccgctgaaag ggcccgactg gaaccgggga tgaaggcgct
     1561 ggacctctgc tgcggaaccg gtgatctggc cctgatgctg gccgaaaggg tcggtccgga
     1621 aggcgaagtg accggggccg atttctcacg gcccatgctc gacctggcgc tcgagaaagc
     1681 caagcaggaa ggcctcaggc aggcgcagtt caaatgggcc gacgcgcttg acctgccctt
     1741 cgaagacgag agctttgacg ccgtgacgat cgccttcggg gcccgcaacc tggccgatct
     1801 cgacaagggg atcagcgaga tgcgccgggt cctggtgccc gggggaagac tggtgatcct
     1861 cgagatcacc cagccccgcc gtcagcccct ggccggcttc ttcggcctct ggttcgaccg
     1921 gatcgtgccg atgctcggca aggtcgccgg cgattcctcc gcctacacct acctgccgga
     1981 atccgttcgc agcttccccg acgccgagac cctggccgga cgcctcgacg cagccgggct
     2041 caggcatgtc cgctggactc tgatggcggg cgggatcatc gcccttcact caggggtgcg
     2101 gtgagcgggg ccgacgagga agcctcccgc cgccgctcgc tgcccgagcc ggtcaccgct
     2161 gttacggaca ccgccgggga ctggctgccg gctcgcatgg ccgaggtcga agccgggctt
     2221 ctcgccgcgg tggaagggga gaccgagctt tccggggatg ccgtccggac actgaacgcc
     2281 ggcggcaagc gaatgcgccc gatgctggtc ctgctctgcg ccggacccga ggccggtgac
     2341 gctgcggtca gggcggggat cgcggtcgag ctggtccaca tggcgagcct ggttcacgac
     2401 gacgtgctgg accgagcccc gctccgccgc gggctgccga ccgtatacgc gagcgcggga
     2461 cgggagcggg cgacatcgct cggtgacagc ctcttctcga ccgccttccg gatcctggtt
     2521 gacggcggcg acgcggatgc ggtgaccgag gtgagcgaca cggcggtcga tctcgcccgg
     2581 ggtgagctca agcagcgcga aggcgccttc gacatggaac tgaccgaggc cgactacttc
     2641 gagcgctgcg ggctcaagac cggccgtctc tttgaggctg cctgcgtgct cgggcgggcc
     2701 agcaccggcg gcgagcccgg gccggtcggt cttttcggcc accggatcgg catcgccttt
     2761 cagttgctcg acgatgtgct cgacatcgcc ggcccgccgg agcgcaccgg caaggcacgc
     2821 ggaaccgacc tgctcgacgg cacggtgacc ctgccgctga tcatcgccgc cggattcgac
     2881 ccggccctgg ccgcactcga cctgcgttcc ctgaacgagg agacggcggc cgtcgcctgc
     2941 gaccggatcg ccgccaccgg cgctctcgac caggtgcgaa ccagggcgat cggcctgatc
     3001 gacgaagcga aggcggggct ggccggagcc ggtctcgagg accagcagcg ggagctcctc
     3061 gagttgatcg ccgacggcgt cgtgatgcgg tactcgtgag ttccccggcg cgccgcggcg
     3121 gcatcccgtc agtccacacc gtttgggata ctgcccgctc atggtcctga gcgacaagac
     3181 cctgcgggag gagatcgagg ccggacggat cgtcatcgat cccttcgagg aacggctgat
     3241 ccagccttcg agcatcgacc tgcgggtgga ccacagctac cgggtcttca acaactcgag
     3301 gtatccgttc attgacgtga agcagccgat ggaagacctg accgaactgg taaagccggc
     3361 cgacgaggag cccttcatcc tgcaccccgg ggagttcgtc ctcggacaga ccctggagcg
     3421 cgtcaccgtc ccggatgaca tggtcgcccg gctggaaggc aagagttcgc tcggccgtct
     3481 cgggctgctg atccattcga ccgccggttt cgtcgacgcc ggtttctcgg gcaacctgac
     3541 gctcgagctt tccaacgtgg ccaatctgcc gatcacgatc tacaagggca tgccgatcgg
     3601 ccagctctcc ttcatgcgca tggaccggcc ggttgacgcc gcatacggct cgaccgacaa
     3661 gggctccaag taccagggcc agcgaggccc gacgccgtca aaggcgtacc tcaacttccg
     3721 ctagcctgcg cttttagctt gtcgacgccc accgcacccc cttataggtg ggaagtaggg
     3781 catttcagcc cgcgtttcat cgatgttcat ttttgaaacg tagacctaac tcttatgcga
     3841 atgacggtaa ttggcaccgg ctacctcggt gcaacccacg ccgcctgcat ggccgagctc
     3901 ggccacgagg tgctcggcgt ggacgtggat gagaaaaaga tcgagtccct ggcaaag
//
LOCUS       DAOTIP010000095         3943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_27204_length_3943_cov_3.471708, whole genome shotgun sequence.
ACCESSION   DAOTIP010000095 DAOTIP010000000
VERSION     DAOTIP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27204_length_3943_cov_3.471708"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..2443
                     /locus_tag="P5138_02730"
     CDS             311..2443
                     /locus_tag="P5138_02730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV59519.1"
                     /translation="MAAPEGLYRIRPFEMVVRAHPPERPGEFRDVAELWTLDKDEFGS
                     RGRIDTTMGVGMAAAAWAPLRSLLEADVPDRLELADAEVLDLLDGAAERLEASGVVLE
                     WDPDLDQTLAGKVVVGSSGRLSQSVPDVFDQNALQGFSWQLSLGGVPLSEEEMALVAD
                     ADGPIARLREKWVLVPRVMADRARNPDLKPLTAIDALSVALTGSTVVDGQRVEVEAKG
                     WVAQLREQVTGDGDQELIEPPGALNATLREYQVEGMRWLHRMTSLGLGGCLADDMGLG
                     KTVMLISLHLHRRLNPQTAGPTLVVCPASLLGNWEREIRRFAPGESVRRYHGPKRDLD
                     QIASGFVLTTYGTMRLDAEKFEQVPWGLLVADEAQHVKNPRSAGAKALRTIPARSRVA
                     LTGTPVENNLSELWAILDWTTPGLLGSLGSFRKKWADPIEMEGSESATRLLNRLVRPF
                     LLRRRKSDPGIAPELPPKTITDQPVELTPEQIKLYEAAVRENMLQIRTAEGINRRGLV
                     LKLLTSLKQICNHPAQFLGEDGTPLAERSGKLELLDELLDTILSEDSSVLVFTQYVAM
                     ARLLSRHLKERGIDAMLLHGGTAVRQRERMVERFQDGEVPVFLLSLKAAGTGLNLTRA
                     DHVIHYDRWWNPAVEEQATDRAHRIGQQNSVQVHRLIAEGTIEDRIASIHETKRELAD
                     AIVSTGEAALTELSNEDLAELVELRNAG"
     gene            2443..3084
                     /locus_tag="P5138_02735"
     CDS             2443..3084
                     /locus_tag="P5138_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV59520.1"
                     /translation="MAEANSGTSRTWRGQTIQERRAGRRERLIDAGIELFGTRGYRNT
                     PVKAICEEAGLTERYFYEAFEDRESLLDAINSMIHEDCRAATVKAIEDSADDLEESMR
                     DGLTAFAQELTRDPRRARIQEIEVVGVSPEMEHKRRETIHTFAGIVADRVRELGGREE
                     VGPLQLDVVSLGLVGSVNEQLIDYVLGYIEIEIDELIENQVAAFACVAGQFLK"
     gene            3133..>3943
                     /locus_tag="P5138_02740"
     CDS             3133..>3943
                     /locus_tag="P5138_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59521.1"
                     /translation="MSNSQGTTPKTIVITGSGRGIGLATATILASRGHRLIMSDIDGD
                     LAGQEAEKLSTRAAGFGLDVTNRDAFREHLEMVESEFGPIDVLVNNAGIAPASPNILE
                     QDPAMTDRTIDINLKGVMNGTIEAIRLMLPRGRGQVVNIASLAGITGVAGLAAYSASK
                     FGVVGFTESVRSEFYDSGLKFTCVMPGPVSTGMMDGTSSSPVVSMLSPEEMAAGIAGA
                     IESGKPRVSLPHSSYVLSRLISILPPGVGIKVSKWTRIDKIYTHITPGARDE"
BASE COUNT          751 a         1291 c         1277 g          624 t
ORIGIN      
        1 ctccggtgcc ctggtcgcgc ttggaatcgc caagaccgcg atgggactgc ccatcttcgc
       61 gctcgccatt tacctgagct acctgatgct gcgggcagcc ggtatcgacc tcaaacagag
      121 gcctgaagcc gaagacgccg gttgaccgaa gggggtgacg aagcccgccg cgcggttgac
      181 atacaatgtc tggccgcctt tgtctccgtc ttcatcaccc ccaagaaccg gcgaagcgcc
      241 cggacgtgcc ggcgccaatg gcgacgcgag cgcgcgtccc cggcgcaagg aggtaccgat
      301 ccgcctccgc gtggccgccc ccgaaggcct ctaccggatt cgtccgttcg aaatggtcgt
      361 tcgcgcccat ccgcccgagc gtcccgggga gttccgggat gtcgccgaac tctggaccct
      421 tgacaaggac gagttcggat cgagaggacg aatcgacacg acgatggggg tggggatggc
      481 cgccgccgcc tgggctccgc tgcgcagcct ccttgaagct gatgtccccg accggcttga
      541 actggccgac gcggaagtgc tcgacctgct cgacggtgcc gccgaaaggc tcgaagcgtc
      601 aggagtggtt ctggaatggg accccgatct cgaccagaca cttgccggca aggtcgtggt
      661 cggcagcagc ggacgactca gccagagcgt gccggatgtc ttcgatcaga acgcgcttca
      721 gggattctcg tggcagctct cgctgggcgg cgttccgctt tccgaggagg agatggcact
      781 tgtcgccgac gccgacggcc cgatcgcgcg gctcagggag aagtgggttc tggtgccgcg
      841 ggtcatggcg gaccgtgccc gcaacccgga cctgaaaccg ctgaccgcca tcgacgcgct
      901 cagcgtcgcc cttaccggga gcaccgtggt cgacggtcag agagtcgagg tcgaggcaaa
      961 ggggtgggtc gcccagctcc gggagcaggt gaccggtgac ggcgaccagg aactgatcga
     1021 gccgcccggg gcgctgaacg ccaccctgcg cgagtaccag gtggaaggca tgcgctggct
     1081 ccaccggatg acctcgctcg gactcggcgg atgcctcgcc gatgacatgg gtctcggcaa
     1141 aacggtcatg ctgatctcgc ttcacctcca ccggcgcctc aacccgcaga cggccggccc
     1201 gaccctggtg gtctgcccgg cctcgctgct cggaaactgg gagcgggaga tccgccgctt
     1261 cgctcccggc gagtccgttc gccgctacca cgggccgaag cgcgacctgg accagatcgc
     1321 cagcggcttt gtgctcacca cctacggcac gatgaggctt gacgcagaga agttcgaaca
     1381 ggtcccatgg ggtctgctgg tcgccgacga ggcccagcac gtcaagaatc cccgctccgc
     1441 cggagccaag gcacttcgca cgattcccgc ccgctccagg gtcgccctca ccggtacgcc
     1501 agtggagaac aacctcagcg agctctgggc gatcctcgac tggacgaccc cgggcctgct
     1561 gggcagcctc ggctcgttcc gcaagaagtg ggccgacccg atcgaaatgg agggcagcga
     1621 gtccgcgacc cgcctgctca accgcctggt gcggcccttc ctgctgcggc gccgcaagtc
     1681 cgatcccggg atcgcgccgg agttgccgcc gaagacgatc accgaccagc cggtcgagct
     1741 cactcccgaa cagatcaagc tctacgaggc tgcggtccgc gagaacatgc tccagatccg
     1801 gaccgccgag ggaatcaacc ggcgcggcct cgttctgaag ctcctgacct cgctgaaaca
     1861 gatctgcaac cacccggccc agttcctcgg cgaagacggg actccgctcg ccgagcggtc
     1921 aggcaagctc gagttgctcg acgaactgct ggacacaatc ctcagcgagg acagctccgt
     1981 actcgtcttc acccagtacg tggcgatggc caggttgctt tccaggcacc tcaaggaacg
     2041 cggcatcgac gcgatgctgc ttcacggcgg cactgcggtc cggcagcgcg agcgcatggt
     2101 ggagaggttc caggacggcg aggtgcccgt cttcctgctt tctctgaagg cggccggcac
     2161 cgggctcaac ctgacccggg cagatcacgt gatccactac gaccgctggt ggaacccggc
     2221 ggtggaagag caggcgaccg accgggccca ccggattggt cagcagaact cggtccaggt
     2281 gcaccgcctg attgccgaag gcacgatcga ggatcgcatc gcctcgatcc acgagaccaa
     2341 gcgggaattg gcggacgcga tcgtcagcac cggcgaggcc gccctgaccg aactcagcaa
     2401 cgaagacctg gccgaactgg tcgagttgag aaacgcaggc tgatggccga agccaactca
     2461 gggacaagca ggacctggcg cggacagacg atccaggaac gtcgtgccgg gcgcagggag
     2521 cgactgatcg acgccgggat cgagctgttc gggacccggg ggtacaggaa caccccggtc
     2581 aaggcgatct gtgaggaagc cggcctgacc gagcgctatt tctacgaggc cttcgaagac
     2641 cgcgagagtt tgcttgatgc catcaactcg atgattcacg aagactgccg tgcagcgacg
     2701 gtgaaggcga tcgaagactc tgccgacgac ctggaggaat cgatgcggga cggactgacc
     2761 gccttcgccc aggaactgac ccgcgatccc cgccgggcgc gaatccagga aatcgaagtg
     2821 gtcggggtga gcccggagat ggagcacaag cgtcgtgaaa ccatccacac cttcgccggc
     2881 atagtcgccg accgtgtcag ggaactcggc gggcgcgaag aggtcggccc cttacagctc
     2941 gacgtcgtct ccctgggcct ggtgggctcg gtgaacgaac agctgatcga ctatgtgctc
     3001 gggtacatcg aaatcgagat cgacgagctg atcgagaacc aggtggcggc attcgcctgc
     3061 gttgccggcc agttcctgaa gtagcgcgca agaactgaca acgcttcgtg tctcatatgc
     3121 caaaatgaat ccatgagtaa ttcccagggc actacgccaa agacgatcgt cattaccggg
     3181 tccggacggg ggatcggctt ggccactgcc acgatcctgg cctcccgcgg ccaccgcctg
     3241 atcatgtccg acatcgacgg agacctggcc gggcaggaag cggagaaact cagcaccaga
     3301 gcggccggat tcggcctcga cgtgaccaac cgcgatgcct tcagggaaca cctggagatg
     3361 gtggaatcgg agttcggacc gatcgacgtc ctcgtcaaca acgcggggat tgcgccggct
     3421 tcgcccaaca tcctcgagca ggatccggcc atgaccgacc ggacaattga catcaacctc
     3481 aagggcgtga tgaacgggac catcgaggcg atccggctca tgctgccccg gggtcgcgga
     3541 caggtcgtca acatcgcttc gcttgccgga atcaccggtg tcgcggggct ggccgcctat
     3601 tccgcttcga agttcggcgt cgtcggcttc accgaatcgg ttcgctcgga gttctacgac
     3661 agcgggctga agttcacctg cgtcatgccc ggcccggtgt caaccggcat gatggatggc
     3721 acctcttcgt caccggtcgt atcgatgctg tcgccggaag agatggcggc cggcattgcc
     3781 ggggcgatcg aaagcggcaa gcctcgcgtg tcgttgccgc attcgagcta cgtactctcc
     3841 cgcctgatct cgatcctgcc gcccggcgtc gggatcaagg tgagcaagtg gaccaggatc
     3901 gacaagatct acacccacat cactcccggc gccagggacg aat
//
LOCUS       DAOTIP010000096         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_28007_length_3855_cov_3.567895, whole genome shotgun sequence.
ACCESSION   DAOTIP010000096 DAOTIP010000000
VERSION     DAOTIP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28007_length_3855_cov_3.567895"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..569)
                     /locus_tag="P5138_02745"
     CDS             complement(72..569)
                     /locus_tag="P5138_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRV59522.1"
                     /translation="MAENINRVTITGNLTRDPELRQTPSGTAVCTLGVAVNGRRKEGD
                     QWVDKPNYFNVVVWGAQGQNCANYLSKGRPVAVDGRLDWRSWEAKDGSGKRSTVEIVA
                     DTVQFLGSRDSAAPGGHGGYNNGGQDQNGQSQGGGFTPSSDVPADPGDFAPTGGGAQD
                     DDIPF"
     gene            complement(758..1153)
                     /gene="rpsF"
                     /locus_tag="P5138_02750"
     CDS             complement(758..1153)
                     /gene="rpsF"
                     /locus_tag="P5138_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRV59523.1"
                     /translation="MTDSARQYELVLMLDPQVEEADREKLVSETKSAIEANGSLKHED
                     NWGIREMAYEIDRKSEADYRLFRFDAERELLGNLDHTLKIADGVMRFRIFKVNPDDPI
                     LPAPPATAVVMAPTGDDDERRGRGRYRED"
     gene            complement(1833..2861)
                     /locus_tag="P5138_02755"
     CDS             complement(1833..2861)
                     /locus_tag="P5138_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59524.1"
                     /translation="MTGTVKRGRFVGILAFSLVALAILAQGARATFPGDNGRLVFLRT
                     DLTKQKGRSGVYTAGPAGRNLKQLTRSRFDYAPSFDPEATTIAFSHLQTGIEGADGNS
                     PAPVFTIGVNGNELKKVTSYPSGPTFPGSPVYYPVVQTAAAGFSPDGQHILYQANRIF
                     IGTEFVLVDRDGLNPEVIASWPDDEASGPVLSPRGNLVAFTRVAGPGLSRIVLMKSDG
                     SGRRALTSGHLDTVSDFSANGRVVAFTRRIAASGESDVFVKHLGTGKVRRLTHFTRLK
                     TRFGNGAFSAYGGVFSPDGRFIAFTGVRRGLPAIYVARRDGSRVRRITPQDMTATSPD
                     WGPRPSSG"
     gene            complement(2931..>3855)
                     /locus_tag="P5138_02760"
     CDS             complement(2931..>3855)
                     /locus_tag="P5138_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRV59525.1"
                     /translation="IPWTQPDSWRILPTPIREAIFNGLLANDIIGFHTHGYCVNFLAC
                     CQRLMGYEVDFGNLTVIHPGGRTMVRAYPLSIDSDRLERAVHSERVAEIEKEILARRR
                     EHLILRVDRADLSKNVLRGFTAFDTFLTDHPEFRERVTFVAHLQPSRQDVPEYAEYLE
                     RIEALVAVVNHRHGTTDWMPVDLRIYDDFDEAVARYKHCDLLMVNAIFDGMNLVAKEA
                     PVINVRDGVLMLSENTGSHEELKEYALTVNPFDIQDQADTIFRALTMSPEERRERADG
                     LKQIIHSRTPADWIEDQLADIELARSERAGA"
BASE COUNT          692 a         1219 c         1248 g          696 t
ORIGIN      
        1 aagcccgtcg cgcttcgcgc tcccccgaag gccttttccg ggtgtacccg cgccgctcct
       61 gcgatcaggc tctagaacgg gatgtcgtcg tcctgggctc cgccgccggt gggggcgaag
      121 tctccgggat cggcgggcac gtccgaggag ggcgtgaatc cgccgccctg gctctggccg
      181 ttctggtcct ggccaccgtt gttgtagccg ccgtggccgc ccggcgccgc actgtcacgg
      241 gaaccgagga actggaccgt gtcggcgacg atctcgacag tcgaacgctt gccggaaccg
      301 tccttggcct cccaggagcg ccagtcaagc cggccatcaa ccgccaccgg tcggcccttc
      361 gagaggtagt tggcgcagtt ctggccctgg gcgccccaga ccacaacgtt gaagtagttg
      421 ggcttgtcga cccactggtc gccttccttg cggcggccgt tgacggccac cccgagcgtg
      481 cagacagcgg tgccgctggg tgtctgacgc agttccgggt ccctggtcag gttgccggtg
      541 atcgtgaccc tgttgatgtt ctcagccatt gtgtgtttgc tccttatcgc gtatctcgta
      601 catcgcagat cccttccgga cctgcaggtc ggagcaggaa tgtagcgacg cctgtggacg
      661 cttaaaggcg aagggggcga aaactcgccc ccttgatccg caaactgcgg gaaaagcctt
      721 tgacagccgg cagaagcggt agccggtgcg gtgaaactca gtcctcgcgg tagcggccgc
      781 ggccgcggcg ctcgtcgtcg tcgccggtcg gcgccatcac aaccgcggtg gcggggggcg
      841 ccggaaggat cggatcgtcg gggttgacct tgaagatgcg gaagcgcatg acgccgtcgg
      901 cgatcttcag ggtgtggtcc aggttgccga gcagctcgcg ttccgcatcg aagcggaaca
      961 ggcgatagtc ggcttcgctc ttgcgatcga tttcataggc catctcgcgg atgccccagt
     1021 tgtcttcgtg cttgagcgaa ccattggctt cgatggccga cttggtctcg gaaaccagct
     1081 tctcgcgatc ggcttcctcg acctgggggt cgagcatcag tacaagttcg tattggcggg
     1141 ctgagtcagt caaaatcgac gggggactat accttcatcg cccaaattcc gcggtgcgat
     1201 gtggattccc gtcgcagagc cggaagcccg agggggcgag tcccgccggc tggtcacttc
     1261 acccggtatg cgatccggca tccgcaatct gaaagtgaac cgggagcgtg cggtacggct
     1321 ggcagcgatt gcggccctcg cggttctcgg gatctccacc cttcccggcc tgctcaggac
     1381 gcccgaacct ccgccggtcc cggccaacgt cggcttccgg cccggcgaga tatcgcacta
     1441 cctgaaggag ccggctcgtt ccgggacggg gcaggtccct gaacgaaaaa cgaaggacac
     1501 gacgcggtcg aaacgattgg ctgccagcaa gcgcgcggcc aagcaacgtc aggagaggag
     1561 agccatggaa cgcgccaaga tgcgcgagcg gaggtcgaag gatcaggcga aagcgagcag
     1621 gaagcggcgg cggagttccg ggactacggt cggttcatcc gtcgcgatct ccccctcgcc
     1681 gccgaatcct gtcgccgcag ccccggcctc cgtctacgcc ccaccccctg ctcccgcgcc
     1741 cgtcccggcg cccccgccgg cgatggagcc cggtgacggt tcccaggaat tcgctccccg
     1801 ctgagcccgg gaatcaaggg gatcagccaa catcatccgc tggaaggccg cggaccccaa
     1861 tcgggggagg ttgccgtcat gtcctgcggg gtgatcctgc gaaccctgct tccgtccctc
     1921 cgcgcaacat agatggctgg caacccgcgc cggaccccgg tgaaggcgat gaacctgcca
     1981 tccggagaga acacccctcc gtaggcagaa aacgcgccgt tgccaaatcg cgtcttgagc
     2041 cgggtgaaat gagtcaggcg cctgaccttg ccggttccca ggtgcttgac gaaaacgtca
     2101 ctttcaccgg atgcggcaat ccgacgggtg aaggcaacga cccgtccgtt ggccgaaaag
     2161 tccgagacgg tgtcaaggtg accggaagta agtgccctcc ttccgctccc gtcggacttc
     2221 atcagcacga tccggctcag accagggccg gccacgcgcg taaaggcaac cagattccct
     2281 cggggcgaga gcaccggtcc cgacgcctca tcgtcgggcc aggaggcgat cacttcgggg
     2341 ttcaaaccgt cgcggtccac gagaacaaat tcggtgccga tgaaaatacg gttggcctga
     2401 tagaggatgt gctgaccgtc gggactgaaa cctgcggctg cggtctggac aaccgggtag
     2461 tagacgggag aacccggaaa ggtcggaccg cttgggtagc tggtgacctt tttcagttcg
     2521 ttaccgttga ctccaatcgt gaacaccggg gcgggactgt tgccatcggc accctcgatt
     2581 ccggtctgaa gatgcgaaaa ggcaattgtc gttgcctcag gatcaaacga aggcgcgtag
     2641 tcaaaccggc tccgggtcag ttgtttcaga ttcctcccgg ccggcccagc ggtatagacg
     2701 cccgacctgc ccttctgttt ggtcagatcg gtacgaagaa atacgagtcg accattatcg
     2761 ccagggaacg tcgccctggc gccctgggca aggatcgcca acgccacaag cgagaacgcc
     2821 agaatcccga caaatctccc cctcttgacc gttccggtca tggttcaaag ctattccgcg
     2881 gagcgttcgg cgtcaacagg gccgagcgac ctacttgatg cccggcggct tcaggcgccg
     2941 gcgcgttcgg aacgggccag ttcgatgtcg gccagctggt cttcgatcca gtcggcggga
     3001 gtcctcgagt ggatgatctg cttgagcccg tcggcccgct cgcggcgttc ctccggggac
     3061 atggtcaagg cacggaagat cgtgtcggcc tggtcctgga tgtcgaaggg attgacggtc
     3121 agggcgtact ccttcaactc ctcgtgggag ccggtgttct ccgaaagcat caggacaccg
     3181 tcccgcacat tgatcaccgg agcttccttg gcaaccaggt tcatgccgtc gaagatcgcg
     3241 ttgaccatca gcaggtcgca gtgcttgtag cgcgcgaccg cctcgtcgaa atcgtcgtag
     3301 atcctcaggt cgaccggcat ccagtcggtg gtcccgtgac ggtggttgac caccgccacc
     3361 agggcttcga tccgctccag gtactcggcg tattccggca cgtcctgccg ggaaggctgg
     3421 agatgggcga caaaggtgac cctttcccgg aattccggat gatcggtgag aaaggtgtcg
     3481 aaggcggtga agccgcgcag cacgttcttg gagagatcgg cccggtcgac ccggaggatc
     3541 aggtgctccc gccggcgggc aagaatctcc ttttcgatct cggcgacccg ctcggaatga
     3601 accgcccttt ccagccggtc cgagtcgatc gacagcggat aggcccggac catcgtccgg
     3661 ccgccgggat ggatgaccgt caggttcccg aagtcgacct cgtaccccat cagccgctgg
     3721 cagcaggcga ggaaattgac gcagtacccg tgggtgtgaa agccgatgat gtcgttggcc
     3781 agcagcccgt tgaagatcgc ctcgcggatc ggtgtcggca gaatccgcca tgagtcgggc
     3841 tgggtccagg ggatg
//
LOCUS       DAOTIP010000097         3849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_28058_length_3849_cov_3.143648, whole genome shotgun sequence.
ACCESSION   DAOTIP010000097 DAOTIP010000000
VERSION     DAOTIP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28058_length_3849_cov_3.143648"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="P5138_02765"
     CDS             <1..371
                     /locus_tag="P5138_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59526.1"
                     /translation="VEPVAGNLGGIVLPWTAVRKSRIMLRVYRKTHSLKQAAERSRVF
                     NERGFIGIGYNARGARAWLKFGRDLRRLGKVKSVKCKRWKNLPATISVRSRAETDSIL
                     PFGGEDPLKSGKILSFKGDA"
     gene            368..2302
                     /locus_tag="P5138_02770"
     CDS             368..2302
                     /locus_tag="P5138_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59527.1"
                     /translation="MKQMKLVTLFFFAAALAATPLVASADEPAPAGKSRLVFSSGGRI
                     VTVKSDGSGRRVLTRKGKVSGRDMDLFSSGETYDRAPQVSPGGKRILFFRQSDYGRYT
                     EGTSSDFYIDGKTMVMPAAGGKAHRVLGPEKKMSSLRDATWIPGSRNLLAVRRYFKDW
                     WARNSVVVVRPDGKVLRTILSFREPRRYSESSTYYLATELSVSPDGNSFLMTRQDTRY
                     DSSSRLELVNLETGKRRVVRNQAREGEFSPDGSGIVFAAGGSGPEVCYQSKEDCRQPY
                     DLYVAGSDGSGVHRVTRTGYDERSPSFSSDGQQIAFSANKNRPAADLSREVYRINADG
                     TCLNWLTNGSPASADPDWGPGAVAASACAPSVRKPLVEGNPGRLDIRRWGPRVWLGKS
                     YDGALLSSSLTFFGLSLVDYRDCGTFKRVDCAEPVTVAQVPICLVGNFLPSLLQVLNN
                     RKLRKNFKLRYGRTRGIGFRTGYARGFSLSIVFSGQRMILTMQRSGGARSGRIDQIAL
                     IRKLRDLGADAGGRLPVFGVPVSALQSYRQARRAVRKFGRREAAKRLEINRWQLRANL
                     RFRANLRRVGPAKPIGCGGFKGIGDLFGDASSSTRAARTAVRTANSPVVRSLTEPLLN
                     SVLLLAEGNELPRGLLRSLP"
     gene            2299..>3849
                     /locus_tag="P5138_02775"
     CDS             2299..>3849
                     /locus_tag="P5138_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59528.1"
                     /translation="MKTAALIVGLGLASAVFAASTAFATEPAPEPARVVFANGGRVLS
                     IAADGSDRKVLVGKSRNPKNEQAGASNPEVSPDGDRLSFSWVRTRDAGEFSDIWLANG
                     DGTGARRILKGDRNNFYADPTFTPSGDLVVAYFRRSGKRAEAGLARVGNSGKFLKKIL
                     VIRQRRRPFSEWTYVRDARFSPDGSKILYGTTEGAFPDAAWSRLSILNRKTGKSRLLA
                     KKALDGAWSPDGGRIVYTSLTWNEDIEVCGLEAPVCNDAGRLRIINADGSGSRSLVST
                     GGDQRGPDWSGDGRIVFQSAGNVPNAAEAYETFSVKPDGGCLTMLTDGTPASLNPSWV
                     DPPGEAGKASTDPGKCGGRPELTLDVRKPTLKGALADMFWAGARADTRLYTGIFREGR
                     APYLSYLDCSAQRETGCPKPFYIVQASLCAAAGHLPATVGGKPDRIQRGLPVYTYTAA
                     EAGPIAYLLAGRFEVVILGGSKGGVKEVDALRRLSEAKAEGDLPVPRFPAGDIHRMNK
                     VRELFKATG"
BASE COUNT          668 a         1254 c         1287 g          640 t
ORIGIN      
        1 aggtcgaacc ggtggccggc aatctcggcg ggatcgtcct gccctggacg gcggtccgga
       61 agtcccggat catgctgcgg gtttatcgga aaacccattc gctgaagcag gcggccgagc
      121 ggtcccgggt cttcaacgaa cgcggattca tcgggatcgg gtacaacgcc cgcggcgcca
      181 gggcatggct gaagttcggg cgcgacctga ggcggctcgg caaggtcaag tcggtgaaat
      241 gcaagcgctg gaagaacctt cccgcgacaa tcagcgtgcg ttcccgcgcc gagactgatt
      301 cgatactccc gttcggggga gaggacccgc tcaagtcagg aaagattttg tcgttcaagg
      361 gagatgcatg aagcagatga aactggtcac tttgttcttt ttcgcggctg cgctcgcggc
      421 cacaccgctt gtcgcaagtg ccgacgaacc ggcacccgcc gggaagtccc gcctggtgtt
      481 ttccagcggt ggccggatcg tgaccgtcaa gtccgacggc agcgggcggc gggtcctgac
      541 ccgcaagggc aaggtgtccg ggcgggacat ggacctgttc agtagcggcg agacctacga
      601 ccgcgccccg caggtctctc ccggcggcaa gaggatcctc ttcttccggc agtctgacta
      661 cggccggtac accgaaggaa cctcaagcga cttctacatc gacggcaaga cgatggtcat
      721 gcctgccgcg ggcggcaagg cacaccgcgt gctcgggccg gaaaagaaga tgtcctccct
      781 gcgggatgcg acctggatcc ccggcagccg gaatctgctc gccgttcgaa ggtacttcaa
      841 ggactggtgg gccaggaact cggtcgtcgt cgtccggccg gatggcaagg ttctccggac
      901 catcctgagc ttcagggagc cgcggcgcta ctcggagtcg agcacctact acctggccac
      961 ggaactctcc gtctcgccgg acggcaacag cttcctgatg acccggcagg acacccgtta
     1021 cgactcgtcc agccgtctcg agctggtcaa cctggagacc ggcaagcgcc gggtcgtccg
     1081 caatcaggcc cgcgagggcg agttctctcc cgacggcagc gggatcgtct tcgccgccgg
     1141 gggttccggc ccggaggtct gttaccagag caaggaagac tgccgtcagc cctatgactt
     1201 gtacgtggcg ggcagcgacg ggtccggcgt ccaccgggtc accaggaccg gctacgacga
     1261 acggtcacct tccttttcct ccgacggcca gcagatcgcc ttcagtgcca acaagaaccg
     1321 gccggccgcc gacctgagcc gcgaggtcta ccggatcaac gccgacggaa cctgcctgaa
     1381 ctggctgacc aacggctcgc cggccagtgc cgatccggac tggggtcctg gcgccgtggc
     1441 agcgtcggcc tgcgcacctt ccgtccgcaa gcccctggtc gaaggtaacc ccggccggct
     1501 cgacattcgc cgctggggtc cccgggtctg gctcggtaag tcatacgacg gagcgctcct
     1561 ctcttcgtcg ctcacctttt tcggactttc gctggtcgat taccgggact gcggcacttt
     1621 caagcgggtc gactgtgccg aacccgtaac cgttgcccag gtgccgatct gcctggtcgg
     1681 caacttcctg ccaagcctgc ttcaggttct gaacaaccga aagctgagga agaacttcaa
     1741 actgcgatac ggcaggacca ggggaatcgg gttccggacc gggtatgccc gcggcttcag
     1801 cctttcgatc gtcttcagcg gacagagaat gatcctcacg atgcagcggt cgggcggggc
     1861 gcgttcgggg cggatcgacc agattgccct gatcaggaag ttgcgcgatc tgggggccga
     1921 cgccggcgga cggttgccgg tcttcggggt gccggtcagc gccctgcaga gttaccggca
     1981 ggccaggcgc gcggtgagga agttcggccg gcgggaagcg gcgaagcggc tcgagatcaa
     2041 ccggtggcag ctgcgcgcga acctccgctt ccgggcaaac ctgcgtcggg tcggtccggc
     2101 caagccgatc ggctgcggcg ggttcaaggg aataggcgac ctcttcggcg acgcctcatc
     2161 ctccacccgt gccgcccgga cggcggtcag gactgccaac tctccggtcg tgaggtcgct
     2221 gaccgaaccg ctactgaatt cggtcctgct tctggccgag ggaaacgaac tgcctcgcgg
     2281 cctgctccgg agcctgccgt gaaaacggcc gcgctgatcg tggggctggg ccttgccagc
     2341 gcggtcttcg cggcttctac tgccttcgcc accgagccgg caccggaacc ggcccgggtt
     2401 gtcttcgcca acgggggccg ggtgctttcg atagcggccg acggctccga tcgcaaggtg
     2461 ctggtcggca agtcccgcaa cccgaagaat gaacaggcgg gagcttccaa ccccgaggtt
     2521 tcgccggacg gcgatcgact ctccttctcg tgggtccgga cacgcgacgc cggtgaattc
     2581 agcgacatat ggctggcgaa tggtgacggc acaggcgccc ggcgcattct caagggagac
     2641 cggaacaact tctacgccga tccgaccttc accccttccg gcgacctggt cgtcgcgtat
     2701 ttcaggcgct cgggcaagag ggccgaggcg ggcctggcca gggtcggcaa cagcgggaag
     2761 ttcctgaaga agattctggt catccggcaa cgacggcggc ccttctccga atggacctat
     2821 gtcagggacg cccgcttctc cccggacggc tcgaagattc tctacggaac caccgaggga
     2881 gcattcccgg acgccgcctg gagccggctg agcatcctga accggaagac cggaaagagc
     2941 aggttgctgg caaagaaagc cctggatggt gcctggtcac cggacggagg ccggatcgtc
     3001 tacacctctc tgacctggaa cgaagacatc gaggtctgcg gcctcgaagc tccggtctgc
     3061 aacgacgccg gccggctcag gatcatcaac gccgatggtt ccggttcgcg gtcgctggtc
     3121 tcgaccggcg gtgaccagcg aggcccggac tggtcggggg acggcaggat cgtcttccag
     3181 tcggccggaa acgtgccgaa tgccgccgag gcctacgaga ctttctcggt gaagccggac
     3241 ggcggatgcc tgactatgct gaccgacggc accccggcca gcctgaaccc ctcctgggtc
     3301 gatccgcccg gcgaggccgg caaggcgagc acggacccgg gcaagtgcgg gggccgtccg
     3361 gagctgacac ttgacgtccg caagccgacc ctgaagggcg ccctggctga catgttctgg
     3421 gccggagccc gggctgacac ccggctctac acaggaatct tccgcgaggg tcgggcaccg
     3481 tacctgagct acctggattg ctctgcccag agagagaccg gctgcccgaa gccgttttac
     3541 atcgtccagg cgtcgctttg cgcggcggcc ggccatctgc ctgcgacggt cggcggaaag
     3601 cccgaccgga tccagcgcgg cctgcctgtc tacacctaca ccgcagcgga ggccggtccg
     3661 atcgcatacc tcctggccgg tcgcttcgaa gtggtgatcc tcggcggttc gaaggggggc
     3721 gtgaaggaag tcgatgccct gcgccggctt tccgaggcca aagcggaggg tgacctcccg
     3781 gtgccgcggt tcccggccgg ggacatccac cggatgaaca aggttcggga acttttcaag
     3841 gcgaccggc
//
LOCUS       DAOTIP010000098         3846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_28082_length_3846_cov_2.808230, whole genome shotgun sequence.
ACCESSION   DAOTIP010000098 DAOTIP010000000
VERSION     DAOTIP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28082_length_3846_cov_2.808230"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="P5138_02780"
     CDS             complement(<1..251)
                     /locus_tag="P5138_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRV59529.1"
                     /translation="MDSILAPISGSGDSRRATEGSSGRAVGHAVILGLVQGPAELLPI
                     SSSAHLAMLPRLLGWDPDELGPELRKSFEVALHGGAALAL"
     gene            294..2156
                     /locus_tag="P5138_02785"
     CDS             294..2156
                     /locus_tag="P5138_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRV59530.1"
                     /translation="MRRYQLMQMAGDGLLVALSYFLAFQLRFIDDPGNVPERYVTLFL
                     QSVGFVIVGKLIVFYLFGLYQKWWRFVGGRDIARIVQAVSVSSLLLVVLFTVVQPFKH
                     DLPRSVAVTDFLLTLFLIGGARLATRLFKERPNKGNRIARGREVLVIGAGSGGQMVVR
                     ELRLNPHLGETAIGFVDDNKDMRGMRIQGISVLGTTDEIETILDETEPDEVMIAIPSA
                     PGVLRARVVAACRDRHIPVRTLPTVFELLRGGVQLSQQLREVQVEDVLGRDPVTMELA
                     RVGAYLEDQVVMVTGAGGSIGKELCRQIGLVGPKLLVMLDHAEDNLFEIEREMVSERH
                     FSRVEAVLADCKETERMFEVMQRFKPNIVFHAAAYKHVPLMESNPLEAVRNNSFATRI
                     TADCAAAAGAERFVLISTDKAVNPQTVMGASKAMAEWAVAAADVRHANTRFVAVRFGN
                     VLGSSGSVVPIFKRQIEQGGPVTVTHPDMTRFFMTIPEAVQLVIRSGDTGVSRGDVFV
                     LDMGEPVKITELAENMIKLAGREPEKDIAIEFVGARPGEKLHEELFGEEERPQSTASK
                     RIMRAVREHPIDPDWVIQTLDRLEQSVQTGDEANLAETVVQLASNPGIDPIRVG"
     gene            2275..2994
                     /locus_tag="P5138_02790"
     CDS             2275..2994
                     /locus_tag="P5138_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRV59531.1"
                     /translation="MTDVIQTVGAYAGLVALLAMGVLAFLVFSQARDIRRLRDWAGGA
                     PERDAEVREVSEIVAEERSAELKVLADREERRMERAGLVETSYWDRLGRSGRIIAIVA
                     VVLILGAAAAWAATSLLGSDDNSGTGKRAAQSKNVKPAQITVGVLNGTGGTEQGLAAQ
                     YAEVLNKDGFKTGVVTDATDTFTESVVMFKNGQQTEARKVGKAVGIDNFEPVQQDVAD
                     LSSGADVTVVIGTDHGPLPGE"
     gene            2995..3576
                     /locus_tag="P5138_02795"
     CDS             2995..3576
                     /locus_tag="P5138_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59532.1"
                     /translation="MKSRAGFRSLGWLAPLTVGLLLIATVAAFGWSQRLKREPLVIDR
                     VEYIALGQTQNGESPTVFSPNGDCKRDRMSINFRTTRSDTADVDIIGLGGNSIRNLAR
                     DRFFKRYREHTLIWDGRKDDGRVPMTGKYRVRVTMHGEDRVLYLPGYIRLHKYTPRGE
                     SGCADQVEWSYSVKEAGKPARPATKDDFQGNTP"
     gene            3573..>3846
                     /locus_tag="P5138_02800"
     CDS             3573..>3846
                     /locus_tag="P5138_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59533.1"
                     /translation="MIEALVALIAAAALAVAILDQPSMRRSVAMAVAGGLVPILIAAD
                     QWHSGPITELRNSPGMLAVAVLCALALVALIAWVIDRWPVIFPVLVL"
BASE COUNT          687 a         1273 c         1253 g          633 t
ORIGIN      
        1 agggccagcg cggcgccgcc gtggagggcc acctcgaaac tcttgcgcag ttccggaccg
       61 agctcgtccg gatcccagcc gagaagtcgg ggaagcattg ccagatgtgc cgacgacgag
      121 atcggcagaa gctccgccgg cccctgaacc aggccgagga ttaccgcgtg cccgaccgcc
      181 cggcccgagg agccctcagt tgcgcgtcgg ctgtcaccgg aaccggaaat aggagcgaga
      241 atgctatcca cgggatagaa tccggggctt caatgcgacg cccagacttt catttgcgcc
      301 ggtatcagct gatgcagatg gccggtgacg gcctgctcgt cgcgctgtcc tacttcctcg
      361 cgttccagct ccgcttcatc gatgacccgg gcaacgtccc ggagcgctac gtgacccttt
      421 tcctccagtc ggtcggtttc gtgatcgtcg gaaagctgat tgtcttctat ctcttcgggc
      481 tctaccagaa gtggtggcgc ttcgtgggcg gccgagacat cgcccggatc gtccaggcgg
      541 tgtcggtttc aagcctcctg ctggtcgtgc tcttcaccgt ggtccagccg ttcaagcacg
      601 acctgccgcg ctcggtcgcg gtgaccgact tcctgctcac gctcttcctg atcggcggtg
      661 ctcgcctggc gacccggctc ttcaaggagc gtcccaacaa gggcaaccgg atcgcccgcg
      721 gccgcgaggt cctggtcatc ggcgcgggat cgggcggcca gatggtggtg cgcgaactgc
      781 ggctgaaccc gcatctcggc gagaccgcga tcggcttcgt cgatgacaac aaggacatgc
      841 gaggcatgcg catccagggc atatccgtcc tcggtaccac cgacgagatc gagaccatcc
      901 tcgacgagac cgaaccggac gaggtgatga tcgcgatccc ctctgccccc ggtgtgctcc
      961 gcgcccgggt ggtggccgcc tgccgggacc ggcatatccc ggtccgcacg ctccccaccg
     1021 tcttcgagct gctccgcggt ggagtccagc tcagccagca gctccgcgaa gtccaggtcg
     1081 aggacgtgct cggccgcgac cccgtcacca tggagctggc ccgggtcggt gcctacctgg
     1141 aagatcaggt cgtaatggtg accggtgccg gcggatcgat cggcaaggag ctctgccgcc
     1201 agatcggtct ggtcggcccg aagttgctgg taatgctcga ccacgccgag gacaaccttt
     1261 tcgagatcga aagggagatg gtctccgagc ggcacttcag tcgggtcgag gcggtgctgg
     1321 ccgactgcaa ggaaaccgag cggatgttcg aggtcatgca gcggttcaag ccgaacatcg
     1381 ttttccacgc ggcggcctac aagcatgtgc cgctgatgga gtcgaacccg ctagaggcgg
     1441 ttcgcaacaa ctccttcgcg accaggatca ccgccgactg cgccgccgcc gccggggccg
     1501 agcggttcgt gctgatctcg accgacaagg cggtcaaccc gcagaccgtg atgggcgctt
     1561 cgaaggcgat ggccgagtgg gcggtagccg cggccgacgt gcgccatgcc aatacccgct
     1621 tcgtcgcggt tcgcttcggc aacgtgctgg gctcctccgg cagcgtcgtc ccgatcttca
     1681 agcgccagat cgaacagggc ggtccggtca ccgttaccca tcccgacatg acccgcttct
     1741 tcatgaccat tccggaagcc gttcagctgg tgatccggtc cggggacacg ggcgtcagcc
     1801 gcggcgacgt cttcgtgctc gacatgggcg agccggtcaa gatcaccgag ctggccgaga
     1861 acatgatcaa gctggccggc cgtgaaccgg agaaggacat cgcgatcgag tttgtcggag
     1921 cgcggccggg cgagaaactc catgaggaac tcttcggcga agaggaacgg ccacagtcga
     1981 ccgcgtccaa gcggatcatg agggcggtgc gggaacaccc gatcgacccg gactgggtca
     2041 tccagactct cgaccggctc gagcagtcgg tccagaccgg cgacgaggcg aacctcgccg
     2101 aaacggtcgt tcaactcgca agcaaccccg gcatcgaccc gatccgggtc ggctgacaac
     2161 cggttcaaag cgcattcatc cgccattcct aactaacatg ccccggtcgg cgtttccgga
     2221 gcgaaagctc atcggcaccg gaatcgccgc ccgaatccga aaagtggaga ccagttgacc
     2281 gacgttatcc agaccgtagg tgcttacgca ggcctcgtcg ccctgctcgc gatgggcgtc
     2341 ctcgcgtttc tggtcttctc ccaggcacgc gacatccgcc gcctgcgcga ctgggccggc
     2401 ggcgcgccgg aacgcgatgc cgaggtgcgc gaggtcagcg agatcgtggc cgaggaaagg
     2461 tcggccgagc tcaaggtcct ggccgaccgg gaagagcgac ggatggagcg ggccggcctg
     2521 gtcgaaacct cctactggga ccggctcggg cgcagcggcc ggatcatcgc gatcgttgcc
     2581 gtggtcctga tcctcggcgc cgctgccgcc tgggccgcaa ctagcctgct cggctccgac
     2641 gacaactccg gcaccggaaa acgcgccgcg cagtcgaaga acgtcaagcc ggcccagatc
     2701 actgtgggag tcctgaacgg caccggaggt accgagcagg gcctcgccgc ccaatacgcc
     2761 gaagtcctca acaaggacgg cttcaagacc ggggtcgtca ccgacgccac ggatacgttc
     2821 accgagagcg tggtcatgtt caagaacggc cagcagaccg aggcaagaaa ggtcggcaag
     2881 gcggtcggca tcgataattt cgagccggtg cagcaggacg tagccgatct ttcctcaggc
     2941 gctgacgtga ccgtggtgat cggtaccgac cacggcccgc tgcccgggga gtaaatgaaa
     3001 agccgcgccg gctttcgcag cctcggctgg ctggcgcccc ttaccgtcgg gctgctcctg
     3061 attgccaccg tcgcggcctt tggctggtcg cagcggctca aacgcgagcc actggtgatc
     3121 gaccgggtcg agtacatcgc gctcggccag acacagaacg gggagtcgcc gaccgtcttc
     3181 agtcccaacg gcgattgcaa gcgggaccgg atgtcaatca acttccggac caccaggtcc
     3241 gacacggccg acgtcgacat catcgggctc ggtggcaact cgatccggaa cctggcccgg
     3301 gaccggttct tcaagcgcta ccgcgaacac acattgatct gggacggccg caaagacgac
     3361 gggcgggttc cgatgaccgg caagtaccgg gtccgggtca ccatgcacgg cgaggaccgg
     3421 gtgctctacc tgcccggcta catccgcctc cacaagtaca cccccagggg agagtccggc
     3481 tgcgcggatc aggtggagtg gtcgtactcg gtcaaggaag ccggcaaacc ggcaagaccg
     3541 gcgaccaaag acgactttca ggggaacaca ccgtgatcga ggcgctggta gccctgatcg
     3601 ccgcggcggc gctcgcggtg gcgattctcg accagccgtc gatgcggcgt tcggtcgcga
     3661 tggcggtggc tgggggcttg gtcccgatcc tgatcgccgc agaccaatgg cactccgggc
     3721 cgatcacgga acttcgcaac tcgcccggca tgctcgccgt ggcagtcctc tgcgcacttg
     3781 ccctcgtcgc actgatcgcc tgggtgatcg accgctggcc ggtgatcttt ccggtgctcg
     3841 tcttgg
//
LOCUS       DAOTIP010000099         3845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_28089_length_3845_cov_3.791293, whole genome shotgun sequence.
ACCESSION   DAOTIP010000099 DAOTIP010000000
VERSION     DAOTIP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28089_length_3845_cov_3.791293"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="P5138_02805"
     CDS             <1..931
                     /locus_tag="P5138_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV59534.1"
                     /translation="GMKDEKTIRLFQVDSFTELEETDGGSRLTSGGGNRYDYWRVAVH
                     QFKNDPLTGISAGNYRQSYYQERKTSEDVQQAHSIELQTLGELGLIGGLALVAFVGAV
                     GFGFWVTARGGRGAEAADPALAIAAGGTFLVWLFHTSVDWMHLLPGLTAVAICSAATL
                     LPPLRAISGRRSLPAIVIVAALVALVSVPVLKQLRSLQLQNQAIEKLESDPREALAAA
                     RDSIRLEPSQRSYYFQAAALARLGLYEPARDSLLAAAENNPKDFVAWSLLGDLATRRG
                     LDQAADLYYRRALRLNPRDPGLIRLAQDGSASP"
     gene            complement(955..1938)
                     /locus_tag="P5138_02810"
     CDS             complement(955..1938)
                     /locus_tag="P5138_02810"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRV59535.1"
                     /translation="MKLLITGGAGFVGGHLSERLLKAGHSVHVIDDLSTGSINNISHL
                     KDSPDFSYTIESVDNVQLMAELVDEADMIYHLAAAVGVQLIVDSPIRALETNIRLTEI
                     VLNLASKKGKPVLFTSTSEVYGKSRALPFAEDGDLTFGATDKGRWAYACSKAIDEFLA
                     LAYYRERGLPTVTFRLFNTVGPRQTGRYGMVIPRLVSQALSGRPLTVYGDGEQRRCFC
                     HVSDTVEALVGMLEVETAYGRVFNVGSQEETSIVDLARRIIDRTNSESEIVFVPYEEA
                     YERGFEDMERRIPDLTRVGEEIGWQTSRDLNEIIDDVVAYTQFSSGTATAV"
     gene            2129..3310
                     /locus_tag="P5138_02815"
     CDS             2129..3310
                     /locus_tag="P5138_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV59536.1"
                     /translation="MLRVIARLNMGGPAKHVGLLSGRRMNELGYETILVHGSLAPGED
                     PLLDVAEQEGARMMYVPDLKPPINPVSDLKALVKLVGVVRKFRPDVVHTHTAKGGFLG
                     RAAALGVWPRPIVVHTYHGHVLEGYFSRAKTAVYRTLERAAAKVSDLLIGVSQATVDD
                     LVRLGVSAPEKFKVVPLGLDLDKFAKIDPVPDLEVRRQIGIDDGGLLFVYVGRIAPIK
                     RLDVLLSAVGMARRDGADIGLAIVGDGEARPELEELSRQLGIESSVHFLGYRSDLERI
                     NAAADAVVLSSDNEGTPVSLIEAAASARPVVTTDVGGVRDVVTDRTGIVVPANDPAAL
                     SEGMVTLARDADLRSEMGSQARQHALSSFSADRLVRDIDGVYKDLINHRRSDPAAGES
                     T"
     gene            3440..>3845
                     /locus_tag="P5138_02820"
     CDS             3440..>3845
                     /locus_tag="P5138_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59537.1"
                     /translation="MQTRLAVIDLSDHVRYPMSNEDGTLCLVFNGEIYGYMDLRRELE
                     RAGHRFATDCDAEVVLHGFEEWGPDLFPRLNGMFALALVDSRRNEITLARDRSGIKPL
                     VMTTGPRFAFASDAMALVSAGLSAGEVDRESVS"
BASE COUNT          717 a         1147 c         1188 g          793 t
ORIGIN      
        1 aggcatgaag gacgaaaaaa caatccgtct gtttcaggtt gattccttca ccgagctcga
       61 ggaaacggat ggcggttcac ggttgacttc cggcgggggc aacagatacg actactggcg
      121 tgtcgccgtc catcagttca agaacgaccc cctgaccggg atcagtgctg gaaattatcg
      181 ccagagctat taccaggagc gaaagacttc ggaggatgtc caacaggctc acagcatcga
      241 gcttcagacc cttggcgagc tggggctgat cggcggcctg gccctggttg ctttcgttgg
      301 tgcggtgggc tttggattct gggttaccgc gcggggcggg cgcggcgccg aagcggcgga
      361 tcccgccctg gcaattgctg ccgggggaac cttcctcgtc tggcttttcc acacgagcgt
      421 tgactggatg cacttgctgc cgggattgac cgcggtggct atctgttccg ccgccacgtt
      481 gctaccccct ttgcgtgcca tctctggccg gcgttccctg ccggcaatcg tgattgtcgc
      541 ggcgctggtc gcactggttt ctgtcccggt tctgaaacaa ctccgctcgc tccagcttca
      601 gaaccaggcc atcgagaagc ttgagtcgga ccctcgcgag gctctcgcgg cggcgcggga
      661 ttcgatccgc ctcgagccgt cgcaacgctc ttactacttc caggcggcag ccttggcacg
      721 gctcgggttg tacgaacccg ccagggacag tcttctcgca gccgccgaga acaacccgaa
      781 ggatttcgtc gcctggagcc tgctgggcga tcttgcgacc agacgcggac tcgaccaggc
      841 cgcggatctc tactaccgtc gcgcgctccg gctcaacccg cgtgatcccg ggctgatcag
      901 gttggcgcag gacgggtcag caagcccctg acccgggagc ttcccgcgac gcggtcagac
      961 ggcagttgcc gtgccggaag agaactgcgt ataggcaacg acgtcgtcga tgatctcgtt
     1021 cagatcacga ctggtctgcc agccaatctc ttctccgact cgcgtcaggt ccggaattct
     1081 ccgttccatg tcctcgaagc cgcgttcgta ggcttcttcg tagggcacga agacgatctc
     1141 ggattccgaa ttcgtgcgat cgattatgcg acgggcaaga tcaacgatcg aagtttcttc
     1201 ctgggagccc acattgaaga cgcgaccgta ggcagtctcg acttccagca tcccgaccag
     1261 ggcctcgacg gtgtcagaca catggcagaa gcaacggcgt tgttcaccgt cgccatacac
     1321 ggtcagaggg cggcctgaaa gggcttgcga aacgaggcgg gggataacca tcccgtagcg
     1381 acccgtctgc ctcggtccga ccgtgttgaa caaacggaag gtgacagtcg ggagtcctcg
     1441 ctcccggtag taggcgaggg ccagaaactc gtcgattgcc ttggagcagg catatgccca
     1501 ccggcccttg tccgtggccc cgaacgtaag gtcgccatcc tcggcgaaag gcagtgcccg
     1561 actcttgccg tacacctcgc tggtcgatgt gaagagcacc ggtttgccct tcttgctcgc
     1621 cagattcaag acaatttcag tcagccggat gttcgtttcg agggcacgaa tcggactgtc
     1681 gacaatcaac tgaaccccta cggcggcggc caggtggtag atcatgtcgg cttcatcgac
     1741 gagttcggcc atcaactgga cgttgtccac cgattcgatc gtgtaggaga aatcgggtga
     1801 gtctttgaga tgggaaatat tgttgatgct gcccgtggac agatcatcga ttacgtgaac
     1861 ggaatgtccg gccttgagca atctctccga gagatgaccg ccgacaaatc ctgccccgcc
     1921 agtgatgaga agcttcattt tccttcgctt tgcggttgct tgccctgctt agtcttccgg
     1981 gcagggatca atcgtcgaaa caccatacag actcaaaagt tcctaaagac tcacggcagt
     2041 tttcggccct catcggtagc ctcaaagcgg ttccgacatg acatcaccag accccataca
     2101 gaaagcggtt tcagaccctc ccgtccgggt gcttcgggtt atcgcccgcc tcaacatggg
     2161 cggaccggcc aagcatgtgg ggctcctgag cggcaggcga atgaacgagc ttggctacga
     2221 gacgattctg gttcacggct cgctcgctcc gggggaggat ccgctgcttg acgtggccga
     2281 gcaggagggt gcccgaatga tgtacgtgcc cgatctgaag ccgccaatca accctgtctc
     2341 ggacctgaag gcgctagtga aactggtggg tgtcgtgcgc aagttccggc cggatgtggt
     2401 ccatacccac acggccaagg gaggtttcct ggggcgagcc gcggcgttgg gcgtatggcc
     2461 tcgcccgata gttgtccaca cctaccacgg acatgttctc gagggttatt tctcccgggc
     2521 caagactgcg gtatatcgaa ccctcgaacg cgccgcggcg aaggtttctg acttgctgat
     2581 cggcgtcagc caggccacgg ttgacgatct ggttcgcctc ggggtttcgg ctcccgagaa
     2641 gttcaaggtc gtgcctctgg ggctggatct cgacaagttc gcgaagatcg atccagtccc
     2701 ggacctcgaa gttcgcaggc agatcggcat tgatgacggc ggacttctat tcgtctacgt
     2761 cggcaggatc gccccgatca agcggcttga cgtcctgctt tccgcagtcg gcatggcacg
     2821 cagggacggt gctgacattg gcctggcgat tgtcggcgac ggagaggctc gaccggaact
     2881 ggaggagctt tcccgccagc tcggaatcga atcgtcggtg cattttcttg gatatcgaag
     2941 cgacctcgaa cgaatcaacg cggctgcgga cgcggtggtg ctgagttctg acaatgaagg
     3001 caccccggtt tccctgatcg aagcggcggc atccgcacgt ccggtcgtga cgactgacgt
     3061 cgggggcgta cgtgacgtgg ttaccgatcg gaccggaatt gtcgtaccag ccaacgatcc
     3121 ggcagccctt tccgagggga tggttacctt ggcccgggat gccgacctca gatccgaaat
     3181 gggatctcag gccaggcagc atgcactttc gagcttttcg gccgaccgcc tcgtccgcga
     3241 catcgacggc gtttacaagg acttgatcaa tcatcgtcgg tcggatccag ctgccggtga
     3301 atcgacctga atgtgcggga ttgccggatt cgagttgcgt ggcggcgatg gcgaggtcgt
     3361 cggcgggagc ttgctccgct cactcaggaa ccggggcccg gacggcgatt tttttgagca
     3421 gcgaggcggg tacggtttgg tgcagacccg gctcgccgtc atcgatctct cggatcacgt
     3481 ccgttacccg atgtcgaacg aggacggcac cctgtgtctt gtcttcaacg gagagatcta
     3541 cggctacatg gatctgcgac gggaacttga gagagccggt cacagatttg ccacggattg
     3601 cgacgccgag gtcgtgcttc atggtttcga ggaatggggc ccggatctct tcccgcgact
     3661 gaacgggatg ttcgcgctgg ccctggtcga cagccgccgc aacgagatca ctctcgcccg
     3721 cgatcggtcc gggatcaaac cactggtcat gaccaccggc ccgcgattcg cattcgcatc
     3781 cgacgctatg gctctggtat ccgccggact cagcgccggt gaagtcgacc gcgaatcggt
     3841 gtccg
//
LOCUS       DAOTIP010000100         3806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_28455_length_3806_cov_3.215676, whole genome shotgun sequence.
ACCESSION   DAOTIP010000100 DAOTIP010000000
VERSION     DAOTIP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28455_length_3806_cov_3.215676"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5138_02825"
     CDS             complement(<1..701)
                     /locus_tag="P5138_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV59538.1"
                     /translation="MLDENRLAVLNAVCDTIVPSLTRDDDPTGFWARSATDTGANQAV
                     ADAISEMPEADQSGMEELLDGLEMQNFSALSQVSREQILTNMSLANREAAIGVAALTQ
                     LTLFMHYGIPPNPSWEQFGFPGPTSAPPEVEKPIRPLTPSDGDVLEADAVIVGSGAGG
                     GVIAARLAEAGLKVIVLEMGGYFNEADFDQTELNGFARMYWRGGPSYTADFNVSVQAG
                     SCLGGGTLINWTNCL"
     gene            complement(788..1528)
                     /locus_tag="P5138_02830"
     CDS             complement(788..1528)
                     /locus_tag="P5138_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HRV59539.1"
                     /translation="MYLLFLDESGIPGEDGIFALGGVAIRVDQWAEVKDRFESCLDEA
                     GWPAEKEFKWSEINTSTDAENASYPVYDCLSDMPLSCLVTVLYTDGEDPEFHERYFAD
                     ADTIYRTALTFVAERFQLFLAKQDSWGVIVLDSRRYEEDDRIRRYFDRIHKEGTDFAE
                     LDRIVDGLLLGPSHYSLGLQLADLVVGPTRAAQFQGLGTSSRCFKKLKPNFMTHPDTG
                     RLVGVGLKIFPLPKTEEDKEEDRLFRPA"
     gene            1600..1890
                     /locus_tag="P5138_02835"
     CDS             1600..1890
                     /locus_tag="P5138_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59540.1"
                     /translation="MEKLEGCDDRALILEDTHLSALRWGAEEARVPLTEIESGQVLRH
                     DKGKLLGVFGKKEERVRTDFGRLDLAIWVPLERQDEAERFVGHVNQAAARVT"
     gene            complement(1909..2373)
                     /locus_tag="P5138_02840"
     CDS             complement(1909..2373)
                     /locus_tag="P5138_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59541.1"
                     /translation="MRNPFTIGAATLAVVLSLGLAACGSSDDSGDSGEDTLTKSELIT
                     QADQICQKGNQELQKLAADAFPGNSQPSTSEVASFISDEIVPHYRDEVDQLRALNPDE
                     ADSSDWNNVVDTFEKELDAVEEDPQVAAKSSDPFTESSAAAKDFGLKVCGAS"
     gene            2448..2951
                     /locus_tag="P5138_02845"
     CDS             2448..2951
                     /locus_tag="P5138_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HRV59542.1"
                     /translation="MENLPLAVTGYQLSVWIHITAVVVGLGMTFAEAVLFPVAMKLDP
                     RHLPYVHRLQLTINRYVASPALLLILVTGIYQVSDGNWDFGSFWISATFAVVILIGGI
                     NGAYFIPADKRLEAMITGEIEAAGPGEFTPSEEYMRQARIEGIIGAVTGILIIISIYL
                     MVVKPGA"
     gene            complement(3001..3552)
                     /locus_tag="P5138_02850"
     CDS             complement(3001..3552)
                     /locus_tag="P5138_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59543.1"
                     /translation="MTVFEPTLSSEKLAAATGRNWDEWLELLDQAGAAKRTHTEIARW
                     LTDDQGVPGWWAQSITVGYERARGLREPGQRADGWSVTASKTVEVPVERLFEAFERDS
                     VREQWLPEAELHLRTATAPVSARYDWGDGTTRLIVGFESLGPDKSRVALEHEKLPDGE
                     VAERMKVWWRERVVALKELLESA"
     gene            3619..>3806
                     /locus_tag="P5138_02855"
     CDS             3619..>3806
                     /locus_tag="P5138_02855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV59544.1"
                     /translation="MTETTEQEVQVRDATDASRYEIEVDGMLAGFSEYRLDGDVITFI
                     HTEIEPAFGGQGLGSKLVG"
BASE COUNT          643 a         1175 c         1294 g          694 t
ORIGIN      
        1 ttcaggcagt tggtccagtt gatcagggtg ccgccaccga gacagctccc cgcctggacg
       61 ctcacgttga aatcagcggt gtaggacggg ccgccgcgcc agtacatccg ggcgaagccg
      121 ttcagctcgg tctggtcgaa gtcggcctcg ttgaagtagc cgcccatctc gaggacgatc
      181 accttcaggc ccgcttcggc cagccgggcg gcgatcacgc cgccgccggc tccggagccg
      241 acgatcaccg cgtccgcctc gagcacgtcc ccgtccgaag gggtcagtgg ccggatcggc
      301 ttctctacct ccggaggggc ggaggtcgga ccggggaagc cgaactgctc ccaggagggg
      361 ttcgggggga ttccataatg catgaagagg gtgagctggg tgagcgccgc gaccccgatt
      421 gccgcttcgc ggttggcgag cgacatgttg gtcaggatct gctcgcggga gacctgcgag
      481 agggcggaga agttctgcat ctccaggccg tcgagcagtt cttccatgcc ggactgatcg
      541 gcctcgggca tctcgctgat ggcgtcggcg acggcttggt tggcccccgt gtcggtggcg
      601 gaccgggccc agaacccggt cgggtcatcg tcccgggtca gcgacggcac gatcgtgtcg
      661 cagacggcgt tcaggacggc aaggcggttc tcgtcaagca ccagggtgct cctctctcct
      721 cgtttcctct cctgcacgcc aagtgtagcg ccgggaaggt cccgccccga tcatccgaag
      781 agggtgacta ggcaggccgg aacaggcggt cctcttcctt gtcttcctcg gtcttcggca
      841 ggggaaagat cttgagtccg acgccgacga gccggccagt gtcggggtgg gtcatgaaat
      901 tcggcttgag cttcttgaag cagcgtgagg acgtgccgag cccctggaac tgggccgccc
      961 tggtgggtcc gacgacaaga tccgccagct gaagtccgag tgagtaatgg gaagggccga
     1021 gcaggaggcc gtcaacgatc cggtccagtt cggcgaagtc cgttccctcc ttgtggatcc
     1081 ggtcgaagta cctgcggatc cggtcgtctt cctcgtagcg gcgggaatcg agcacgatca
     1141 cgccccagga atcctgcttg gccaggaaga gctggaatct ctcggccacg aacgtgaggg
     1201 cggtgcggta aatcgtgtcc gcatcggcga agtacctctc gtgaaattcc gggtcctctc
     1261 cgtccgtgta gagaacggtg accaggcagc tgaggggcat gtccgagagg caatcgtaga
     1321 ccggatacga ggcgttctcc gcatccgtgc tggtgttgat ctccgaccac ttgaactcct
     1381 tctcggcggg ccagcctgcc tcgtcgaggc atgactcgaa gcgatccttg acctcggccc
     1441 actggtcgac ccgtatcgcc actccgccca gggcgaagat tccgtcctca ccggggattc
     1501 cggactcgtc gaggaagagc aggtacatgg gcggcaggct atccccggag gcgggaagaa
     1561 cgactggtca ggccgggcct gagcggatac gttcgcagca tggaaaagct ggagggttgt
     1621 gacgatcggg cgctgattct ggaggacacc cacctgtccg cccttcggtg gggagccgaa
     1681 gaggcgcggg tgccgctgac cgagatcgag tcaggtcagg tgctgcgcca tgacaagggc
     1741 aagctgctcg gggtcttcgg caagaaggag gagcgggtga ggaccgactt cggccgtctc
     1801 gacctggcga tctgggtgcc gctggagcgg caggatgaag ctgagcggtt cgtcggccac
     1861 gtgaaccagg ctgccgcccg agtcacctga ccgacgaacg cccgagactc agctggctcc
     1921 gcagaccttc agaccgaagt ccttggcagc ggcgctggat tccgtgaacg gatcgctgct
     1981 cttggctgct acctggggat cttcctcgac cgcgtcgagt tccttctcga aggtgtcaac
     2041 gacgttgttc cagtccgagc tgtcggcctc gtccgggttg agggcacgca gctgatcgac
     2101 ctcgtcgcgg tagtggggga cgatctcgtc gctgatgaag cttgcgacct cgctggtgct
     2161 gggctgggaa ttaccgggga aggcgtcggc ggccagtttc tggagttcct ggttgccttt
     2221 ctggcagatc tggtcggcct gggtgatcag ctcggacttg gtcagcgtgt cttcaccgga
     2281 atctccggaa tcgtcgctgg aaccacaggc ggcgaggccg agggaaagca cgacggccag
     2341 cgtggccgcg ccgatggtga aggggtttct cattgagacc tttcgaatgg gcttgagacg
     2401 gaaccgggcc atcgtaaagg ctccggcggg cgggataggg tcgggtcatg gagaacctgc
     2461 cgctggccgt gaccggctat cagctgtccg tgtggattca catcaccgcc gtggtcgtcg
     2521 ggctcgggat gaccttcgcc gaggccgtgc tcttcccggt cgcgatgaag ctggaccccc
     2581 ggcatctgcc gtatgtccac cggcttcagc tgaccatcaa ccggtacgtc gccagccccg
     2641 ccctgctgct gatcctggtc accggcatct accaggtcag tgacggcaac tgggacttcg
     2701 gttcgttctg gatcagcgcc accttcgcgg tcgtgatcct gatcggcggc atcaacggcg
     2761 cctacttcat ccccgccgac aagcggctgg aagcgatgat cacgggggag atcgaggcgg
     2821 ccgggcccgg cgagttcacc ccgtcggagg agtacatgcg gcaggcccgg atcgagggga
     2881 ttatcggggc ggtcacaggg atcctgatca tcatttcgat ctacctgatg gtggtcaagc
     2941 ccggcgcctg acctctcggg gcgtcggatc gggcgcgagg ccaccggagg accgggcggg
     3001 ctatgcggac tcgagcagtt ccttgagggc gacgacccgc tcccgccacc agaccttcat
     3061 gcgctcggcg acctcgccgt cggggagctt ctcgtgttcg agggcgaccc ggctcttgtc
     3121 cggcccgaga ctctcgaagc cgacgatcag tcgggtggtg ccgtcccccc agtcgtaacg
     3181 ggccgaaaca ggggcggtgg cggttcgcag gtgaagttcc gcttccggaa gccattgctc
     3241 ccggaccgaa tcccgctcga aagcttcgaa gagtctctcg acggggacct cgaccgtctt
     3301 tgaggcggtg accgaccagc cgtcggcccg ctggcccggt tcgcgcagtc cacgggcccg
     3361 ctcgtaaccg accgtgatcg actgcgccca ccagccgggc acgccctgat catcggtcag
     3421 ccagcgggca atctcggtgt gggtccgctt tgcagcaccg gcctggtcga gtagctcgag
     3481 ccactcgtcc caattccggc cggtcgctgc ggcgagcttc tcgctcgaga gcgtcggttc
     3541 aaagacggtc atgactcaaa gtcatttaac cacccggggg cgggccgaaa cagaaaatgc
     3601 gcttactaac attcagtcat gaccgaaacc acggagcagg aagtccaggt tcgtgacgcc
     3661 acggatgcca gccgctacga gatcgaggtc gacgggatgc tcgccggatt ctcggagtac
     3721 cgccttgacg gtgacgtcat caccttcatc cacacggaaa tcgaaccggc cttcggcggg
     3781 caggggctgg gatcgaagct ggtcgg
//
LOCUS       DAOTIP010000101         3782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_28703_length_3782_cov_3.024685, whole genome shotgun sequence.
ACCESSION   DAOTIP010000101 DAOTIP010000000
VERSION     DAOTIP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28703_length_3782_cov_3.024685"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="P5138_02860"
     CDS             <1..653
                     /locus_tag="P5138_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV59545.1"
                     /translation="DVARLGKPGSYQIPAEVGGLPTATLVIDDGSKDGTEEVAEAHGA
                     VVIRHVVNRGGGAALRTGYRLMADSDAAIVVTLDADGQHLPAEMPNLVQPVLDGKADV
                     AHGSRVLGHAEQNHFARELGIVFFNRLVSFITRTHVTDCSNGYRAVRTSVLPTLVLKQ
                     EQFHTSEFMIEAIKRGVPAVEVPVTVEQRLHGHSKKPAVFRYGMGFANAIVRTWLR"
     gene            complement(961..1242)
                     /locus_tag="P5138_02865"
     CDS             complement(961..1242)
                     /locus_tag="P5138_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRV59546.1"
                     /translation="MGYEYLFIALLFGIATGIIGRAKGGSFFIWLIVGTILPGLGLIA
                     VFMMRNEINEPERQCPNCGKILKLYVQVCPRCGYDLYLPPPEETRMPPR"
     gene            complement(1298..2677)
                     /locus_tag="P5138_02870"
     CDS             complement(1298..2677)
                     /locus_tag="P5138_02870"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV59547.1"
                     /translation="MAWNIVIVGGGFAGANVARHLEKVLPRQSSRLTLVNDVNFLLYT
                     PLLPEAAAGTLEPRHVVTPLREILHRTNLRLGVVDDHDRESREVTLRSHMGQTEQIPY
                     DHLVLAPGSISRFLPVPGLNEHAVGFKSLADAIFLRNHLIETLEMANATDDPELREEL
                     LTYVFVGGGYAGLEALAELQDFAADAMRDYPKARLHGMRWILVEAADRVLPEIDIELA
                     DYAVDELRGRGIDIKLGTTLEEVTETTATISTGEVVPTRTVVWTAGVVPNPSLQNFGV
                     PLDERGRVKVDSSMAVEGMDGIWSLGDSAAVPNPKGGVCPPTAQHAVRQAPVVAANIA
                     AAIGLGEPRVFDYQGYASFVNLGRYKAVGKISDRTFRGFKAWWMARSYHMSQIPGASR
                     KARAVIDWTASLPFSRDISEVGSIGRPGVLSPDRYIHGGSHRPVDGEVPAAGRDRAQA
                     DVNGTGEAD"
     gene            2752..>3782
                     /locus_tag="P5138_02875"
     CDS             2752..>3782
                     /locus_tag="P5138_02875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HRV59548.1"
                     /translation="MGHTSRVGGGIFGAIALAAFLMAILISSSSTASAAGIVDLGSPP
                     PKVSKVAANSGQVVSETVLNPRSADGFWTPERLAAAKPAEMPTPADSLDIPKFASGAS
                     ASAATGDFTPGNVTRFPQVVHGKIFFSVGSDTYSCSGTIIDSLNRNVIFTAGHCVFDQ
                     STKSFVTNLIFIPGYENGNAPLGVGNAVKLFTTSQWANQGANSYDMGVVVLDQPAESF
                     FGARQIAFDLNPKGRQYTLYGYPSRPNPAYNGEVLQGCHSAFAGRDNGGGNITPYPIA
                     AHPCYMQQGASGGGWVTLGNYVNSVVSYGYCDSLPSSCGYIFGPYFSNAAKSLYVNAG
                     GSPRPTVKVL"
BASE COUNT          657 a         1247 c         1196 g          682 t
ORIGIN      
        1 ccgacgtggc ccggctgggc aaaccgggca gctaccagat cccggccgag gtcggcggac
       61 tgccgaccgc gaccctggtc atcgacgacg gctcgaagga cggaaccgaa gaagtggcgg
      121 aagcccatgg cgcagtcgtg atccgccacg tcgtaaatcg cgggggtggc gccgccctgc
      181 gcacgggcta ccggctgatg gccgattccg atgccgcgat cgtggtcacc ctggacgccg
      241 acggccagca cctgccggcc gagatgccga acctcgtcca gccggtgctc gacggcaagg
      301 cggatgtcgc ccatggctcg cgtgtcctgg gtcacgcaga gcagaaccac ttcgcccgtg
      361 aactcgggat cgtcttcttc aaccgcctcg tctccttcat cacgcggacc catgtcacgg
      421 actgctcgaa tggctaccgg gcggtccgca cctcggtcct ccccaccctc gtcctgaagc
      481 aggagcagtt ccacacctcc gagttcatga tcgaggcgat caagcgcgga gtgccggcgg
      541 tcgaggtacc ggtcacggtc gaacagcgcc tccacggcca ttcaaaaaag ccggctgtct
      601 tccgctatgg aatgggtttc gccaacgcca tcgtccgcac ctggctcagg tagccggcct
      661 tgcgggcgcg ggacggggtt gcccgttttg gtccgtcgct tcgctccccc gaaggcccaa
      721 aacgggcaac cccaccccac gccatctcgg atcgaccatg attccgtgac ggtgttgtct
      781 gtgggtgagc tcctgatccg gtcgggtccg ggtcgggtgt gccgctcagg ggccttcggg
      841 ggagcccgca ggcccgaacc cgggtcccgc tgggtccggt tcgggtgtgc ccctcaaaaa
      901 cctcggaatg gggagcgaag cgacggtttt tgaggggtac acccgggccg ggcccctcga
      961 ttacctggga ggcatgcggg tttcttctgg cggcggcagg tagaggtcgt agccgcaccg
     1021 ggggcagacc tggacgtaga gcttgaggat ctttccgcag ttcgggcact gacgttccgg
     1081 ctcgttgatc tcgtttctca tcatgaatac cgcgatcagg ccgaggccgg gcaggatggt
     1141 gccgactatc agccagatga aaaacgaccc gcccttggcg cggccgatga ttccggtcgc
     1201 gatgccgaac agcagggcga tgaacagata ctcgtaaccc acagcgacat tgtgccgtca
     1261 gcgggttcgg cgtgacggcc gggactgctc gtgcgggtca gtctgcttcg ccggtaccgt
     1321 tcacatcggc ctgggcccgg tcccgtccgg ctgccggaac ctcgccgtcc accggccggt
     1381 gggagccccc gtggatgtag cggtcgggac tcaggactcc gggccgaccg atcgaaccca
     1441 cctcggagat gtcacgtgag aagggaaggc tggcggtcca gtcgatcacc gcgcgggcct
     1501 tgcgcgatgc acccgggatc tggctcatgt ggtacgagcg ggccatccac caggccttga
     1561 atccgcggaa ggtccggtcg ctgatcttgc ccacggcctt gtaacgaccg aggttcacga
     1621 aagaggcata accctgatag tcgaaaacgc ggggttctcc gaggccgata gccgcggcga
     1681 tgtttgctgc cacgaccggt gcctgccgta ccgcgtgctg ggcagtcggc gggcagaccc
     1741 cgcccttggg gttgggaacc gcagcggaat cccccaggga ccagatgccg tccatgccct
     1801 cgacagccat gctgctgtcc accttcaccc ttccccgctc gtcgagcgga accccgaagt
     1861 tctggagact gggattgggc actacccctg ccgtccagac gacggtccgg gtcgggacca
     1921 cttcaccggt ggagatcgtg gccgtggtct cggtgacctc ttccagcgtc gtcccgagct
     1981 tgatgtcgat gccgcggccc cgcaactcgt ccacggcgta gtcggcaagt tcgatgtcga
     2041 tctccggcag gacccggtcc gcggcctcga caaggatcca cctcatgcca tgcagccggg
     2101 ccttcgggta atccctcatc gcatcggcgg cgaagtcctg gagctcggcc agggcctcga
     2161 gccctgcata tccgccgccg acgaagacat aggtgagcaa ttcctcgcgg agttccgggt
     2221 catcggtggc gttggccatc tcgagggtct cgatcaggtg gttcctgagg aagatcgcgt
     2281 cggcgagact cttgaatccg accgcgtgct cgttcaggcc gggcacggga aggaagcggg
     2341 aaatcgagcc tggcgccagg accaggtggt cgtaggggat ctgctcggtc tgccccatgt
     2401 ggctgcgcag ggtgacctcg cgggactccc ggtcgtggtc gtcgaccaca ccgagacgga
     2461 gattggtccg gtggaggatc tccctcaggg gcgtcaccac atggcgcggt tccagggtgc
     2521 cggctgcagc ttccgggagc agcggcgtgt agagcaggaa attgacgtca ttgaccaggg
     2581 tgagccgcga cgactgacgg ggaagaacct tctccaggtg cctggcgacg ttcgctcctg
     2641 cgaatccgcc gccgacgatg acgatgttcc atgccatggc ggcgagtata gggcgcaaag
     2701 ttcgcggaca aatacgcaac caacggcccc ccgatctgtt ctaatgacac tatgggtcac
     2761 acaagcaggg tcgggggagg aatattcggg gcaatcgccc tggcagcctt cctcatggcc
     2821 atcctcatct cgtcttcatc caccgccagc gccgccggaa tcgtcgatct cggctcgcct
     2881 ccgccaaagg tctcgaaggt ggcagccaat tcgggtcaag tcgtttccga aacggtcctc
     2941 aatccccgat cggcagacgg cttctggacg ccggaacgcc ttgccgccgc caagcccgcc
     3001 gagatgccca cgccggcgga cagccttgac atcccgaagt tcgcttccgg tgcctctgcc
     3061 tcagccgcga ccggcgattt caccccgggc aacgtgacca ggttcccgca ggttgttcac
     3121 ggcaagatct tcttctcggt cggatcggat acctacagtt gctccggcac gatcatcgac
     3181 tcgctgaacc gcaacgtgat cttcacagcc ggccactgcg tcttcgacca gtccaccaag
     3241 tcgttcgtca ccaacctcat cttcattccc ggctacgaga acggaaatgc gccgctcgga
     3301 gtcggaaacg cggtcaagct tttcaccacc tcccaatggg cgaatcaggg ggcgaactcc
     3361 tatgacatgg gtgtcgtcgt actcgatcag ccggccgaga gtttctttgg cgcacggcag
     3421 atcgccttcg acctgaatcc gaagggccgc cagtacacgc tttacggata tccgtccagg
     3481 cccaatcccg cctacaacgg cgaagtgctg cagggctgcc attcggcttt tgccggtcgc
     3541 gacaacggcg gcggcaatat cacgccctac ccgattgccg cccacccctg ttacatgcag
     3601 cagggcgcca gcggaggtgg atgggtgact ctgggcaact acgtgaattc ggtcgtcagc
     3661 tacggatact gcgacagctt gccgagctca tgcggataca ttttcggccc ctacttctcc
     3721 aacgcggcga agtccctgta cgtcaatgcc ggaggctctc cgcgcccgac cgtcaaggtt
     3781 ct
//
LOCUS       DAOTIP010000102         3736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_29176_length_3736_cov_2.872046, whole genome shotgun sequence.
ACCESSION   DAOTIP010000102 DAOTIP010000000
VERSION     DAOTIP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29176_length_3736_cov_2.872046"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5138_02880"
     CDS             <1..830
                     /locus_tag="P5138_02880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV59549.1"
                     /translation="ETMDETGVGEAVAWAESEGWQPGLGDHRPFFAADPEGFFRSVTD
                     GETVATISVVRGSPDVAFVGLYIVAAELRGQGLGKQLWDETLGRFEGLTLGLDAVPEQ
                     VATYASEGFEPAYGNARFSAEAADLPDPEPGSPIQPATSVPFEDLVAFDAGHYFGPRP
                     DFLSQWMNGEGRQGLVATNDGEITGFAASRPTSTGNRIGPFFAANKDTARDLLLALAG
                     RLTGPISVDAPEPNRNAFDLFTNLGMEKSFPTTRMYRGPAPDLPLDRIYGITTLELG"
     gene            954..1502
                     /locus_tag="P5138_02885"
     CDS             954..1502
                     /locus_tag="P5138_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59550.1"
                     /translation="MMFSAMRRMSTGRLTGSVVAAVAITVSLFMVESPAEARWKQPGC
                     TKFSKQIRTANTPAAKRAARKRLAKCKVNRRVYGILKNKMIAGTRADGVYVDSVYCAN
                     GSFSYDGGESFVKKGWRVENARIRGRNITAIVRGKIKGGSYVTAVARRGSQWKVGWES
                     FGQARDLGDAELTNARALCRKA"
     gene            1528..2238
                     /locus_tag="P5138_02890"
     CDS             1528..2238
                     /locus_tag="P5138_02890"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HRV59551.1"
                     /translation="MPLLPGATVRDRALACAGALAGVALIGISANLAGHELHDQTWIL
                     APVGASAVLVFAVPATPLAQPWPVIGGSTISALTGVVCVHLISPEPLAAGVAVAAAIA
                     VMTVARCLHPPGGAAALTAVLAATTDTDIGTLFPVVPILLDSLAIVALAWVFHRVVTG
                     HSYPHRALVPSPGPGVKAGEGQFTDADLDAVLGRVGSTYDINREDLEAILDAVQTEAA
                     QRRERQKQTGAAPPRGQT"
     gene            2283..>2628
                     /locus_tag="P5138_02895"
     CDS             2283..>2628
                     /locus_tag="P5138_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59552.1"
                     /translation="MAAREQKKSDRLNRLMGEAVDVIDGIFHVYRAWWADILTLSLII
                     FVPLGLLGAADSHALESIGPGHDVKVLALIVGALAVTATGVLGEVFLAGAIGLSLTHA
                     EDGRPPGIRFLTK"
     assembly_gap    2629..2638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2639..3084
                     /locus_tag="P5138_02900"
     CDS             <2639..3084
                     /locus_tag="P5138_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59553.1"
                     /translation="GPLIVVDVLYVLSIAFGFVLLIIPGFAALVYFALAGPIVEIEQQ
                     GVKSAFRRSFRLVRGSYWLVFMILVPIEVLGESVRKGIEAACEALLGHSFIAVGLGEA
                     LAEVALSPLFAIAVVVLTRKMMQQKEGTILPGPSPRQLKSGKLSA"
     gene            3210..>3736
                     /locus_tag="P5138_02905"
     CDS             3210..>3736
                     /locus_tag="P5138_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV59554.1"
                     /translation="MSTSIADDSVAAEEITTAVSERRGWMLYQTVRLILRPLRWWARI
                     QVKGLEHLPGKGGALIVSNHDSWLDPLAIIEAMMWKQRQLRFLAKHTLWKFRPLAWIL
                     DEAAQIPIRRGESDTAALEAAVDAAGRGETIGIFPEGTLSRGTNLRARRGISRLARAC
                     PGTPVVLVAVTGGTDL"
BASE COUNT          618 a         1287 c         1205 g          616 t
ORIGIN      
        1 ttgagacgat ggacgagacg ggggtcggcg aggcggttgc ctgggccgag tccgagggct
       61 ggcagcccgg tctcggtgat caccggccct tcttcgccgc cgaccccgaa ggcttcttcc
      121 gctcggtaac ggacggcgag acggtcgcca cgatctcggt cgtccgcggt tccccggatg
      181 tcgccttcgt cggtctgtac atcgtggccg ctgagttgcg ggggcaggga cttggcaagc
      241 agttgtggga tgagactctt ggacgtttcg aaggtctgac ccttggcctc gacgcggttc
      301 ccgagcaggt cgccacctac gcgtctgaag ggttcgagcc cgcctacgga aacgccaggt
      361 tctcggccga ggccgctgac cttccggacc cggaaccggg ctcacctatc cagcccgcca
      421 cctcggtgcc cttcgaagat ctggtcgcct tcgacgccgg ccactacttc ggcccgaggc
      481 ccgatttcct gagccagtgg atgaacggag agggccgcca gggcctggta gccacgaacg
      541 acggcgagat caccggcttc gccgcctccc gcccgaccag caccggcaac cggatcggcc
      601 ccttcttcgc cgccaacaag gacaccgccc gggacctgct cctcgccctc gccggacgct
      661 tgaccggccc gatctcggtc gacgcccccg agcccaaccg gaacgccttc gacctcttca
      721 caaacctggg catggagaag agcttcccca ccacccgcat gtaccgaggc ccagccccgg
      781 acctccccct cgaccggatc tacggcatca ccaccctcga actcggctga ccgaccccgg
      841 ccgagaccag tagccggcca ctcgcggccg ggggacgttc tcccggtcca gttcatatgg
      901 ttggcgtggt ccgggggaag acgaactcac gagcaagacc gaaaggcggg cagatgatgt
      961 tctcagcgat gcgcaggatg agcaccggca ggctgaccgg ttcggtggtg gcagcagtcg
     1021 ccatcacggt ttcactcttc atggtcgaat caccggccga ggccaggtgg aagcagccgg
     1081 gctgcaccaa gttcagcaag cagatccgga cagccaacac cccggcggcc aaacgggccg
     1141 cgcgcaagcg actcgcaaaa tgcaaggtca accgccgcgt ctacggaatc ctcaagaaca
     1201 agatgatcgc cggcaccagg gccgacgggg tgtacgtcga cagcgtctac tgcgccaacg
     1261 gctccttcag ctacgacggc ggggaaagtt tcgtgaagaa aggctggcgg gtagagaacg
     1321 cccggatcag gggcaggaac atcaccgcga tcgtgcgagg aaagatcaag ggcgggtcct
     1381 atgtgaccgc cgtggctcgt cgcggcagcc agtggaaggt cggctgggaa tccttcggcc
     1441 aggccaggga tctcggtgat gccgagctga ccaacgcgag ggcgctttgc cgcaaggcct
     1501 gacgtgacgg agggcaagtg gtggttcgtg ccgcttctgc ccggcgcgac ggttcgcgac
     1561 cgggcccttg cctgtgccgg tgccctggcc ggggtcgcgc tgatcggaat cagcgcgaac
     1621 ctggccggtc acgagcttca cgaccagacc tggatcctgg cgccggtcgg agcttcggcc
     1681 gtactcgtct ttgccgtccc cgccacgccg cttgcccagc cctggccggt gatcgggggc
     1741 agcacgatct cggccctgac cggggtcgtc tgcgttcacc tgatctcccc cgaaccactg
     1801 gcggcgggag tggccgtggc tgcggcgatc gcggtaatga cggtggcccg ctgcctccac
     1861 cctcccggag gggcggccgc gctgaccgcg gtcctcgccg cgaccaccga caccgacatc
     1921 ggaaccctct tcccggtggt tccgatcctg cttgactcgc tggcgatcgt cgccctggcc
     1981 tgggtgtttc accgggtcgt gaccggccat tcctaccccc accgggccct cgtcccctct
     2041 cccggaccgg gcgtcaaggc aggcgaaggc cagttcacag acgccgacct cgacgccgta
     2101 ctcggacggg tcggcagcac ctacgacatc aaccgcgagg acctggaagc gatcctcgat
     2161 gcggtgcaga ccgaggcggc ccagcgccgc gagaggcaga aacagacagg agcggccccg
     2221 ccccggggcc agacctgacc ctcacgggca tttcggcccg ttgctgaaag actttccggc
     2281 gcatggccgc ccgtgagcag aagaagagcg atcgcctgaa ccgcctcatg ggcgaggcgg
     2341 tcgacgtcat cgacggaatc ttccacgtct accgcgcctg gtgggcagac atcctcaccc
     2401 tctcgctgat catcttcgtt ccgctcgggc tgctgggcgc cgccgactcc cacgcgctcg
     2461 agtcgatcgg tcccggccac gacgtgaaag tactggccct gatcgtcggc gccctcgcgg
     2521 tgacggccac cggcgtcctc ggggaggtat tcctggccgg tgcgatcggg ctttcgctga
     2581 cccatgcgga ggacggcagg cccccgggca tccggttcct gacaaagcnn nnnnnnnntc
     2641 ggtcccctga tcgtggtcga cgtcctctac gtgctgtcga tcgccttcgg cttcgtgctg
     2701 ctgatcattc cgggcttcgc cgcgctcgtc tatttcgccc tggccgggcc gatcgtcgaa
     2761 atcgagcaac agggcgtgaa gtctgcgttc agaagaagct tccgactggt ccgcggcagt
     2821 tactggctgg tcttcatgat cctggttccg atcgaggtgc tgggcgaatc ggtccggaag
     2881 ggaatcgaag cggcctgcga agcgctcctc ggccactcct tcatcgcggt cggtctgggg
     2941 gaagccctcg ccgaagtggc tctctccccg ctgttcgcga tcgctgttgt ggtgctgacc
     3001 cggaagatga tgcagcagaa ggaaggcacc atcctgcccg gcccctcccc ccgccagttg
     3061 aagtccggga agctcagcgc ctgagtccag gggccgtagc accggcccgg atcacccgtt
     3121 tcggatgatt tagacaaaca ggcgagtctt catacccaca ggctctagag tcgtgtgttc
     3181 atggatccgc aaggcactgt ggaaagacga tgagtacgtc gatcgccgac gattccgtcg
     3241 cggccgaaga gatcacgacc gcggtctccg aacgacgggg ctggatgctc taccagaccg
     3301 tccgcctcat cctgcggccg ctccgctggt gggcgcgcat ccaggtcaag ggcctcgaac
     3361 acctgccggg caagggcggc gcgctgatcg tctccaacca cgattcgtgg cttgacccgc
     3421 tggcgatcat tgaggcgatg atgtggaaac agcgccagct ccgcttcctc gccaagcaca
     3481 ccctctggaa attccggccg cttgcctgga ttctcgacga agccgcccag atcccgattc
     3541 gccgcggaga gagtgacacc gcggcgctgg aggcagcggt cgatgcggcc ggccgcggcg
     3601 agacgatcgg aatcttcccc gagggcaccc tttcccgcgg caccaacctg agggcccggc
     3661 gcgggatctc ccgactggcc cgggcctgcc cgggaacgcc ggtcgtactt gtcgcagtga
     3721 ccggcggcac cgacct
//
LOCUS       DAOTIP010000103         3724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_29311_length_3724_cov_3.523303, whole genome shotgun sequence.
ACCESSION   DAOTIP010000103 DAOTIP010000000
VERSION     DAOTIP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29311_length_3724_cov_3.523303"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="cydD"
                     /locus_tag="P5138_02910"
     CDS             <1..1431
                     /gene="cydD"
                     /locus_tag="P5138_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HRV59555.1"
                     /translation="AGRFGAARVMSELRERLSRHLLLNRPGALQDEQSGDLVSSAIDG
                     VAALENYFARYLPQMVLAGIVPVAVLAWVVQYNWESAVILAVTAPLIIVFMVLIGLLT
                     EQRTRKRWERMSRLSSHFLDLVGGLATLRANSQAYSQAGSIQAVGEEYRRETMGALRV
                     GFLSSLVLELMAMIGVAMVATAIGIQLANGHLELVAGLTVLLLAPELYMPLRQLGAQF
                     HASADGMAAAERIFEVIDQPVAVTAPAEPVPAPDPARWSVAMEGISFAWPGRERVLES
                     IDLELEPGETVALVGASGGGKSTLAHLLLRLAEPEAGTIRCGGVDLAEVDPREWRRQI
                     AWVPQRPTIFSATFADNLRLFNPDASNAKLTEAIGVAVLGDVVAGLPDGLETMVGEGG
                     RRLSAGQVQRIALARALLSEAPLLVLDEPTAHLDQDTEQELAEAINEAARDRTALIIS
                     HREEPIAKADRTFVLDTRGLSEMVAA"
     gene            1428..3113
                     /gene="cydC"
                     /locus_tag="P5138_02915"
     CDS             1428..3113
                     /gene="cydC"
                     /locus_tag="P5138_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HRV59556.1"
                     /translation="MIAARLERSPLGRAALATRNRWKLFAVSVLLGTGTVLAAVGLLT
                     ASGYLISRAAQRPEILSLTVAIVGVRFFGISRALLRYGERLSSHDLAFRTLTDLRGRF
                     FERLIPLVPAGIDGSRRADLMSRFVGDVDRLQDLYLRALSPPLVAIFCSIFCVALASI
                     ILPVAGLVLAIMLLLGGLAAPALTRWAARSAGHRQSAARGRLAGDLHEIASGGAEIAV
                     AGREEDWLLRTSEDDRRLVSLQRRDAMSGGLSEGLSTLLAVGAAVAVTAASIPAVHSG
                     EVNGVLLAALALLAMASFEAITPLSQAAAVIDATDEAAGRIEEVTERPVPVSDVEHTR
                     PLPAGGAIRLKGVSFNYPGSDREILTRADFELRPGEAVALTGPSGIGKSTLAELMVRF
                     RDPSEGCIGLGGIDIGELDQEELREAVRLAPQDAYMFNTTIAENVKLGRPEADRERIF
                     EALAEVGLEDWINSLPDGIDSLVGEDGSKISGGQRQRIAAARVFLSRARFLIFDEPTA
                     HLDPEGAASLERHLVDRRDTGCGVLVITHTLADPEAFDRILELDEGTFREVRV"
     gene            complement(3131..>3724)
                     /locus_tag="P5138_02920"
     CDS             complement(3131..>3724)
                     /locus_tag="P5138_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRV59557.1"
                     /translation="RWARELHDETLQNLAGLRVLLSGTRRRTAMSPVSPILDQSIERI
                     DETIVELRRLIADLRPAALDELGVEAALSSLVERLSSTGVDIDLYVDLGPADDAHGRY
                     EPLIEDTIYRLIQESLNNAIRHGHAVRAIVEVIEEEGEIGIRVTDDGSGFDPGRTGGG
                     FGLLGMRERVNLTGGRFDLRSGDEGTTITAALPARRA"
BASE COUNT          542 a         1272 c         1285 g          625 t
ORIGIN      
        1 gccggtcggt tcggcgccgc ccgggtgatg tcggagctgc gcgaaaggct ttcgcggcac
       61 ctgctgctga accgcccggg cgcacttcag gacgagcagt ccggtgacct ggtcagttcg
      121 gcgatcgacg gcgtcgcggc ccttgagaat tacttcgccc gctacctccc gcagatggtt
      181 ctggccggga tcgttccggt cgcggtgctg gcctgggtcg tccagtacaa ctgggaatcg
      241 gccgtgatac tcgccgtcac cgcgccgctc atcatcgtct tcatggtcct gatcggtctg
      301 ctgaccgagc agcgaacccg caagcggtgg gagcggatgt cccgtctctc ctcgcatttc
      361 ctcgacctgg tcggcgggct cgcgaccctg cgcgccaaca gccaggccta ctcgcaggca
      421 ggttcgatcc aggcggtcgg cgaggagtac cgccgggaga cgatgggtgc cctgagggtc
      481 ggcttcctct cctcgctggt gctggagttg atggcgatga tcggggttgc catggtcgct
      541 accgcgatcg gcattcagct ggccaacggg cacctcgaac ttgtcgccgg cctgaccgtg
      601 ttgctacttg cccccgagct ctacatgccc ctgcgccagc ttggggccca gttccacgcc
      661 agcgccgacg gcatggcggc ggccgagagg atcttcgaag tgatcgacca gccggtggca
      721 gtgactgccc cggctgagcc agtacccgct cccgacccgg cccgctggtc ggttgccatg
      781 gagggcattt cattcgcgtg gccgggtcgg gagcgggtgc tggagtcgat cgacctcgaa
      841 ctcgagccgg gcgagaccgt cgccctggtc ggagcgagtg gcggcggcaa gagcactctg
      901 gcccacctgc tcctgaggct ggccgagccc gaggcgggga cgattcgctg cggcggggtg
      961 gacctggccg aagtcgatcc gcgtgagtgg cggcgccaga ttgcatgggt gccgcagcgg
     1021 ccgacgattt tttccgccac gttcgccgac aacctgcgcc tgttcaaccc cgatgcgagt
     1081 aacgcgaagc tgacggaagc gatcggtgtc gcggtgctcg gggacgtggt ggcgggactc
     1141 cccgacggtc tcgagaccat ggttggcgag ggcggccgac ggctttcggc cggtcaggtc
     1201 cagcggatag cgctggcccg tgccctgctc tcggaggccc cgctgctggt cctcgacgaa
     1261 ccgaccgccc acctcgacca ggacaccgaa caggaactgg ccgaagcgat caacgaggcg
     1321 gcaagggacc ggaccgccct gatcatctcc caccgcgagg agccgatcgc gaaggccgac
     1381 cgcaccttcg tcctcgacac ccggggcctg agcgaaatgg ttgccgcatg attgccgccc
     1441 gtctggagag atcgcccctg ggccgagccg ccctggcgac caggaaccgc tggaagctct
     1501 tcgccgtctc ggtgctgctc gggaccggga ccgtgctggc cgcggtcgga ctgctgaccg
     1561 cttccggcta cctgatcagc cgggccgccc agcggccgga aatcctgtcg ctgaccgtcg
     1621 cgatcgtcgg ggtgcggttc ttcggcatct cgagggccct gctccgttac ggagaaaggc
     1681 tcagctcgca tgacctcgcc ttccgcaccc tgaccgacct ccgtggccgt ttcttcgagc
     1741 ggctgatccc cctggtcccc gccgggatcg acggctctcg ccgggccgac ctgatgagcc
     1801 gcttcgtcgg ggatgtcgac cggctgcagg acctctacct gcgggccctc tcgccgcctc
     1861 tggtggcgat cttctgctcg atcttctgtg tggccctcgc ctcgatcatc ctcccggtgg
     1921 ccggactggt tctggcgatc atgctgctgc tcggcggact ggcagctccg gccctgaccc
     1981 gctgggccgc ccgcagcgcc ggccacaggc agtcggccgc acgggggagg ctggccggtg
     2041 acctgcacga aatcgcctct ggcggggccg agatcgcggt cgcggggcgc gaggaagact
     2101 ggctgttgcg caccagcgag gacgaccgga ggctggtttc gctccagcgc cgcgacgcga
     2161 tgagcggcgg actgtccgag gggctgtcga ccctgctcgc ggtcggtgcc gcggtcgcgg
     2221 tcaccgcggc atcgatcccc gccgttcatt ccggggaagt gaacggggtg ctgctggccg
     2281 cactggcgct cctcgccatg gcttccttcg aggcgatcac cccgctcagc caggcggccg
     2341 cagtgatcga cgccaccgac gaggcggctg gccgaatcga ggaagtcacc gagcggccgg
     2401 tcccggtctc tgatgtcgag cacacccgtc cgctcccggc cggcggtgca atccggctga
     2461 agggggtgag cttcaactac ccgggcagcg accgcgagat tctgacccgg gcggacttcg
     2521 aactgcgacc cggtgaggcg gtcgccctga ccggtccgag cggcatcggc aagagcaccc
     2581 ttgccgaact gatggtccgg tttcgcgacc cctcggaagg atgcataggc ctgggcggaa
     2641 tcgacatcgg cgagctggac caggaggagc tccgcgaggc ggtgaggctg gccccgcagg
     2701 acgcctacat gttcaacact acgatcgccg agaacgtgaa gctcggtcgt cccgaagccg
     2761 atcgcgaacg gatcttcgaa gccctggccg aagtcgggct ggaggactgg atcaactcgc
     2821 tgccggacgg gatcgactcc ctggtcggcg aggacggatc gaagatctcc ggcggccagc
     2881 ggcagcggat cgccgccgcc agggtcttcc tctcccgggc ccgcttcctg atcttcgacg
     2941 agccgaccgc ccatctcgac cccgagggtg ccgcatcact cgaaaggcat ctggtcgatc
     3001 gccgcgacac cggctgcggg gtactggtca tcacccacac gctcgccgac ccggaagcct
     3061 tcgaccgcat cctcgaactg gacgagggta cgttccgcga agtgcgggtc tgactggctg
     3121 ttcgcggacg tcaggcccgg cgggcgggca gggcggcggt gattgtggtg ccctcgtcgc
     3181 cggagcgcaa gtcgaaccgg ccgccggtca ggttcaccct ttcgcgcatt cccagcagtc
     3241 cgaaaccgcc tccggtgcgg ccgggatcga agccggaacc gtcgtccgtc acccggatcc
     3301 cgatctcgcc ttcttcctcg atcacctcga cgatcgcccg gaccgcgtga ccgtggcgga
     3361 tcgcgttgtt cagtgattcc tggatcagcc ggtagatcgt gtcctcgatc agcggctcgt
     3421 agcgcccgtg ggcgtcatcg gccgggccga ggtcaacgta caggtcgatg tcgactccgg
     3481 tgctggagag ccgctcgacc agcgagctga gcgccgcctc gaccccgagt tcatccaggg
     3541 cggcgggcct caggtcggcg atcagccggc gcagttcgac gatggtctcg tcgatccttt
     3601 cgatcgactg gtcgaggatc ggcgagaccg ggctcatcgc ggtgcgacgg cgggtgcccg
     3661 aaagaagaac gcgaagcccg gccaggttct gaagcgtttc gtcgtggagc tcacgggccc
     3721 agcg
//
LOCUS       DAOTIP010000104         3721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_29332_length_3721_cov_3.845336, whole genome shotgun sequence.
ACCESSION   DAOTIP010000104 DAOTIP010000000
VERSION     DAOTIP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29332_length_3721_cov_3.845336"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="P5138_02925"
     CDS             <1..1304
                     /locus_tag="P5138_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV59558.1"
                     /translation="PEARNAIGPVESRALFEFLGRFRDDEEANVLVVTGAGTEAFCAG
                     ADLKSVVAMFDPDSKLEPLYDGSDLDAHPIPAEGNIGPTRWTSINKPIIAAVNGAAYA
                     GGLEWACLAHLRVADQHASFGVTCRRWNVGLGDGGTQRLPRMIGLSRALDLIITGRVV
                     GAAEAERIGLVNEVTESGRCLERALELAAEISALPQPALRTDLEAALRGFGTPLEEGL
                     AIEAGLFNSLLDQPEIMSGASAFVDRDHPDRVAGAAPLHLPGAAYAFAERAHRGQIDR
                     HGGGDFIEHPSRVARIAVEHGGDDLAEAAAYLHDTVEKADTTPEEIEAAFGPEMRDLV
                     EALGQDPGIGDRASRRADHRHRVAIAGETARLVYLADRLAGIEAMIERIEAGESPSDL
                     DTERRLGLWRGDVEALSGTSPPPGLVADIGDRLDRLEHLMS"
     gene            1466..2503
                     /locus_tag="P5138_02930"
     CDS             1466..2503
                     /locus_tag="P5138_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV59559.1"
                     /translation="MPDVRLTGLTRRYGDFTAVDSIDLDVRQGEFFTMLGPSGSGKTT
                     TLKMIAGFEQPDAGKVELGGQDVSGLAPYARSVNTVFQDYALFPHMTVSANIEYGLSI
                     AKIPKSDRAERRTEALRMVRLEGHGDRKPNELSGGQRQRVALARAIVNRPRVLLLDEP
                     LGALDLKLREQMQVELKQIQSEVGITFIYVTHDQDEALTMSDRIAVFNEGRIEQLSSP
                     RDLYEHPANEFVAGFVGVSNAIERDGKKLTIRPEKIRLIGEGDPTDGLVTERGEITES
                     AYVGMVTRVKVELEQGGAVQVALQNTESEAEPGGRVGQKVVVGWRPEHAVSIQQGERT
                     QSVQLEEENVS"
     gene            2500..3687
                     /locus_tag="P5138_02935"
     CDS             2500..3687
                     /locus_tag="P5138_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV59560.1"
                     /translation="MSYANRHPARFAWLLVLAMVGVLAVAGCGGSSDSNSDEVTSIGQ
                     TEGALNLIAWAGYAEDGSTDKTVDWVTPFEKQTQCKVNTKTASTSDEMVKLMRTGQYD
                     GVSASGDASVRLIEGGDVSPIDMSILSNYADINPSLKDQDYNSKDGQNYGMPHGWGAN
                     VLLYNKDAVQPAPDSWGSVFDPAMASKYSGKITAPDNPIYIADAAVYLEATQPDLGIT
                     DPYELDEDQFNAAVDLLKQQNKFIGEYWPDATKNISSYTNGDSVIGSSWQYQTSALQA
                     AGENVDADLPKEGSTGWSDTWMLSSQAEHPNCMLEWMNWMGSPKVQAQVAEWFGEAPA
                     NLGACDLTSDPNHCDTYHAKDEKYYDQIAFWKTPISDCGDDRGSVCMTYDQWVQAWTE
                     IKG"
BASE COUNT          719 a         1214 c         1212 g          576 t
ORIGIN      
        1 gtccggaggc ccgcaacgca atcggcccgg tcgagtcacg cgccctgttc gagttcctgg
       61 gccggttccg cgatgacgag gaagcgaatg tcctggttgt caccggggcc ggcaccgagg
      121 ccttctgcgc cggggccgac ctcaagtcgg tggtcgccat gttcgacccg gattcaaagc
      181 tggaacccct gtatgacggc tccgaccttg acgcccaccc gatcccggcc gaaggaaata
      241 tcggccctac ccggtggacc tccatcaaca agccgatcat tgccgcggtc aacggcgcgg
      301 cctatgccgg cggactcgaa tgggcatgtc tggcacacct gcgggtcgct gaccagcatg
      361 cgtccttcgg agtgacctgc cgccgctgga acgtcggcct cggcgacggc ggcacccagc
      421 gcctgcccag gatgatcggg ctttcaaggg cactggacct gatcatcacc ggaagggtgg
      481 tcggcgccgc cgaggcggag cggatcggcc tggtcaacga ggtgaccgag agcggacgct
      541 gcctcgaacg ggccctcgag ctggccgcgg aaatctcggc actcccccag cctgcgcttc
      601 ggaccgacct cgaagctgcg ctccgcggtt tcggaacgcc gctggaggaa ggactcgcga
      661 tcgaggcggg acttttcaac agcctgctcg accagccgga gatcatgtcg ggagcctcgg
      721 ccttcgtcga ccgcgaccat cccgaccggg tcgcgggcgc cgccccgctc cacctgccgg
      781 gcgcggctta cgcgtttgcc gagcgcgccc atcgcggcca gatcgaccgg cacggcggcg
      841 gcgatttcat cgagcatcct tcccgcgttg cccggatcgc ggtcgagcat ggcggtgacg
      901 acctcgcgga ggccgccgcc tatctccacg acacggttga gaaagcggac acgaccccgg
      961 aggaaatcga agccgccttc ggccccgaga tgcgtgatct ggtcgaagcg cttggacagg
     1021 accccggaat cggggaccgg gccagccgcc gggccgacca ccgccaccgg gttgcgatcg
     1081 ccggcgagac cgcgcggctc gtttatctcg ccgaccggct tgccgggatc gaagcgatga
     1141 tcgaaaggat cgaagccggt gaatccccga gtgaccttga cacggaacgc aggctcggtc
     1201 tctggagagg tgatgtcgag gcgctttcgg gaacgagccc gccacccggc ctggtggccg
     1261 acatcggcga ccggctcgac cggctcgaac atctcatgtc ctgacccgga ctaccccccg
     1321 ccgctcccag cggttagagt cggattcgtc cgggatagca ggagatctgc cccggatgct
     1381 tcttcagctt caccagtcgc gagagtcgag aggagcaacc acgtccagtc cgtccacacc
     1441 ccgcagtccg gccgaccaga ccagcgtgcc tgacgtccgc ctgaccggcc tgaccaggcg
     1501 ttacggggat ttcacagccg tcgattcgat cgacctcgat gtccgccagg gggagttctt
     1561 caccatgctg ggcccttccg gctccggcaa gacgaccacg ctgaagatga tcgccggatt
     1621 cgaacaaccc gatgcgggca aggtcgaact cggcggccag gatgtcagcg ggcttgcccc
     1681 ttacgcccgc tcggtcaaca ccgtcttcca ggattacgcc ctcttccctc acatgacggt
     1741 ctcggcgaac atcgaatacg gcctttcgat cgccaagatc ccgaagagcg atagggccga
     1801 gcgccggacc gaagctctgc ggatggttcg tctcgagggt cacggggacc gcaagccgaa
     1861 cgagctctcc ggtggccagc ggcagcgggt cgccctggcc cgggcgatcg tgaaccggcc
     1921 gcgggtgctg ctgctggacg aaccgctggg cgccctcgac ctcaagctgc gcgagcagat
     1981 gcaggtcgag ctgaagcaga tccagtccga ggtcggaatc accttcatct acgtgaccca
     2041 cgatcaggac gaagccctga ccatgagcga ccggatcgcg gtcttcaacg agggccggat
     2101 cgagcagctc agctcccctc gggatctcta cgaacacccg gccaacgagt tcgtggccgg
     2161 cttcgtcggt gtctcgaacg cgatcgagcg ggacggaaag aagctgacga tccggccgga
     2221 gaagatccgg ctcataggcg aaggggaccc gaccgatggc ctggtgaccg aaaggggaga
     2281 gatcaccgag tccgcttacg tcgggatggt gacgcgggtc aaggtcgaac ttgagcaggg
     2341 tggcgcggtc caggtcgccc tgcagaacac ggaatctgaa gccgagcccg gtgggagagt
     2401 cggccagaag gtagttgtcg gatggcggcc ggagcacgcc gtttcgattc aacaaggaga
     2461 gagaacgcag tccgtccaat tggaggagga aaacgtttca tgagttatgc gaatagacac
     2521 cccgcccgct tcgcctggct gcttgtgctg gcgatggtcg gcgtgctcgc ggtcgccggt
     2581 tgcggcggca gcagcgacag caacagtgac gaagtcacgt caatcggcca gaccgaaggt
     2641 gccctgaacc tgatcgcctg ggcgggttac gccgaggatg gttcaaccga caagacggtc
     2701 gactgggtga cgccgttcga gaagcagacc cagtgcaagg tcaacacaaa gaccgccagc
     2761 acctcggacg agatggtcaa gctgatgcgg accggccagt acgacggtgt gtcggcatcc
     2821 ggtgacgcct cggtgcgcct gatcgagggc ggagacgtca gcccgatcga catgagcatc
     2881 ctctcgaact acgccgacat caacccgagc ctgaaggacc aggactacaa ctccaaggac
     2941 ggccagaact acggcatgcc ccatggctgg ggagccaacg ttctgctcta caacaaggac
     3001 gcggtccagc ctgctcccga cagctggggt tcggtgttcg atccggcgat ggcctccaag
     3061 tacagcggca agatcaccgc tccggacaac ccgatctaca tcgctgacgc ggccgtctac
     3121 ctcgaggcca cccagcccga tctcgggatc accgatccgt acgagctcga cgaggatcag
     3181 ttcaacgccg cggtggacct gctgaagcag cagaacaagt tcatcgggga gtactggccg
     3241 gatgcgacga agaacatctc gtcgtacacg aacggcgact cggtgatcgg ttcgagctgg
     3301 cagtaccaga ccagcgccct gcaggccgcc ggcgagaatg ttgacgccga cctgccgaag
     3361 gaaggttcga ccggttggtc cgacacctgg atgctgagct cacaggccga gcacccgaac
     3421 tgcatgctcg agtggatgaa ctggatgggt tcgcccaagg tccaggccca ggtggccgag
     3481 tggttcggcg aggcgccggc caatctcggt gcctgtgatc tgacctcgga tccgaatcac
     3541 tgcgacacct accacgccaa ggacgagaag tactacgacc agatcgcgtt ctggaagacg
     3601 ccgatcagcg actgcggcga cgaccgcggt tcggtctgca tgacctacga ccagtgggtc
     3661 caggcatgga cggaaatcaa ggggtgaccg ctggccgctg aggccacagt cactccgccc
     3721 c
//
LOCUS       DAOTIP010000105         3690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_29647_length_3690_cov_3.156534, whole genome shotgun sequence.
ACCESSION   DAOTIP010000105 DAOTIP010000000
VERSION     DAOTIP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29647_length_3690_cov_3.156534"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..2444)
                     /gene="cofH"
                     /locus_tag="P5138_02940"
     CDS             complement(75..2444)
                     /gene="cofH"
                     /locus_tag="P5138_02940"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HRV59561.1"
                     /translation="MRRVTFSRNYTISLSRTCQCYCKYCAFATHQAHVHPPEEVERML
                     DEAVRRRAKELLFLTGEKPEVNPVVKEQLRSWGHEDFTSYVVWACERALERGILPHTN
                     IGAIGEEDLARLREVTASQGLMLESASERLMETVHAGSPTKHPAHRLKTIEAAGRLKI
                     PFTTGILVGIGETPGERTASLEAIAALQRKYGHIQEVIIQNFVPHPRYYGQEPADIAA
                     EASDRRWKESGTVVDLDSGIESEAAQGDPASDGPERDRMGRPTIPLPDWACPVSIEDL
                     KDLIRQTAELMPDVGVQVPPNLSDWWADLVEAGATDLGGLSANGDHISPEHPFPSPHQ
                     VRKELQPLGYALTERLCVYPQYMNADWMEQGVLDVIKLKYWSFIPRLGSGRVSEESFD
                     PGVAVEAIAKGREGEELTEDELTALFSETRPEVVEEMRVAADALRAELAGETATFVVN
                     RNINFTNICTVGCAFCGFGQGRRSPDAYESTEEEFIGRVEEAIEFGATELCMQGGIHP
                     ELDLESYGRWLKLAKRVAPDIHLHAYSPMEINHMCETSGLPPAEVFEYLIDCGLGSTP
                     GTAAEVLDDGVRQRISPNKLPVDRWVEIIEASHRAGLKSTSTVMFGHIEEPRELARHM
                     KVIRDLQARTGGITEFVPLSFIPFNTLLGRTHGVEEISIDENLKHTAVFRLALGRSIT
                     NLQASWVKMGLPGATEALNWGVNDLGGTLMEESISRMAGSQHGVRLEVEELVDAAHRA
                     GRKAAQRNTSYEILQGFPDAVASPPPEMLIDLANPGEEPAAGLADPAAA"
     gene            2544..2981
                     /locus_tag="P5138_02945"
     CDS             2544..2981
                     /locus_tag="P5138_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRV59562.1"
                     /translation="MKASLKFLVVPLAVGALFFAGCGGDDSSDDNSSSDTAPTQSQPA
                     DNSMGSGQVLNLAADPSGQLAYDKTELSAKAGKVTLDFTNESPIGHNVVIEDSSGKEI
                     ASTDTITGQSTTAEFKIKPGTYTFYCSIPGHEQAGMKGTLTVQ"
     regulatory      2983..3050
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3081..3485
                     /gene="crcB"
                     /locus_tag="P5138_02950"
     CDS             3081..3485
                     /gene="crcB"
                     /locus_tag="P5138_02950"
                     /inference="COORDINATES: protein motif:HMM:NF010812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRV59563.1"
                     /translation="MIFRRHPIRKLAAIYAGGVCGALLRVSLGEAFPHGAETWPWPTF
                     AANLIGAFLIGYFFASFSDHTPDQLHHPFFATGICGSLTTFSTMQLELFNMVDAREFL
                     LAATYTATTLAFGYVAVRLGIAAERSGLEVGP"
     gene            3482..>3690
                     /locus_tag="P5138_02955"
     CDS             3482..>3690
                     /locus_tag="P5138_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="may be involved in chromosome condensation;
                     overexpression in Escherichia coli protects against
                     decondensation by camphor; overexpressing the protein
                     results in an increase in supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRV59564.1"
                     /translation="MNVFGVGILGWVLVGMLGGLAAGARYLLDAEISRLTASPFPIGI
                     FAVNMTGAFLLGLVAGSTLHGEALV"
BASE COUNT          599 a         1231 c         1175 g          685 t
ORIGIN      
        1 ggcaggtccc gaagggcaag tctgcccggc tgctcttcga ggaccatggc gcgcatcgct
       61 tcggccctcc ccgcctaggc cgccgcgggg tccgcgagtc cggccgccgg ttcttccccc
      121 gggttggcga ggtcgatcag catctccggc gggggcgagg cgactgcgtc ggggaacccc
      181 tgcaggatct cgtaggaggt gtttcgctga gccgccttcc ggccggcccg gtgcgcggcg
      241 tcgaccagtt cctcgacttc gagcctgacc ccgtgctggg agccggccat gcgggagatg
      301 ctctcttcca tcagggttcc gccgaggtcg ttcacgcccc agttgagtgc ctcggtcgcg
      361 cccggaaggc ccatcttcac ccagctcgcc tgcaggttgg tgatcgaacg gccgagggca
      421 agccggaaga cggcggtgtg cttgaggttc tcgtcgatcg agatctcctc gaccccgtgg
      481 gtgcggccga gcagggtgtt gaaggggatg aagctgagcg gcacgaattc agtgatgcca
      541 ccggtgcggg cctggaggtc gcgaatcacc ttcatgtggc gggccagttc gcgcggctcc
      601 tcgatgtggc cgaacatcac ggttgaggtc gacttcaggc cggcccggtg ggaagcctca
      661 atgatctcga cccagcggtc gaccggcaac ttgttcggtg aaatccgctg acggacaccg
      721 tcgtccagca cttccgcggc ggttcccggg gtcgaaccga gaccgcaatc gatcaggtac
      781 tcgaagacct cggccggagg caggcccgag gtctcgcaca tgtggttgat ctccatcggc
      841 gagtaggcgt gaaggtgaat gtccggggcg acccgcttgg ccagcttcag ccagcgaccg
      901 taggactcga ggtccagttc cgggtggatc cctccctgca tgcagagctc ggtggcgccg
      961 aactcgatcg cctcctcgac ccgcccgatg aactcttcct cggtcgattc gtaggcatcc
     1021 ggtgaacggc gtccctggcc aaagccgcag aaagcgcagc cgaccgtgca gatgttggtg
     1081 aagttgatgt tgcggttgac cacgaaggtg gcggtctcgc cggcgagttc ggcccgcagc
     1141 gcgtcggccg ccacccgcat ctcctcgacc acctccggcc gggtctcgga gaagagcgcg
     1201 gtcagctcgt cttcggtcag ctcctcgcct tcgcggccct tggcgatcgc ttcgaccgcc
     1261 accccgggat cgaagctctc ctcgctgacc cgccccgaac cgagccgggg gatgaagctc
     1321 cagtacttca gcttgatcac gtccaggacg ccctgctcca tccagtcggc gttcatgtac
     1381 tgcgggtaca cgcagagccg ctcggtcagc gcatagccga gcggctgaag ttccttgcga
     1441 acctgatggg ggctcgggaa gggatgctcc ggggagatgt ggtcgccgtt cgcggagagt
     1501 ccgccgaggt cggttgcccc ggcctcgacc aggtccgccc accagtcgga aaggttgggc
     1561 ggcacctgta ccccgacgtc gggcatgagt tcggcggtct gccggatcag gtccttgaga
     1621 tcttcgatcg aaaccggaca ggcccagtcc ggcaggggaa tggtcggccg gcccatccgg
     1681 tcgcgttccg gtccgtcgct cgcaggatcg ccctgggccg cctcgctctc gatcccggaa
     1741 tcgaggtcaa ccaccgtgcc cgactccttc cacctccggt cggaagcctc tgccgcgatg
     1801 tcggccggct cctggccgta atagcggggg tggggaacga agttctggat gatcacttcc
     1861 tggatgtggc cgtacttgcg ctgaagcgcg gcaatggcct ccagcgaagc ggtccgctcc
     1921 cccggcgtct cgccgatccc gaccaggatg ccagtcgtga aggggatttt cagccggccg
     1981 gccgcctcga tcgtcttcag ccggtgggcg ggatgtttgg tcggagaacc ggcatggacg
     2041 gtttccatca ggcgctccga cgccgactcg agcatcagtc cctgggaggc ggtcacctcg
     2101 cgcaggcgag ccaggtcctc ctccccgatc gcaccgatgt tggtgtgcgg gaggatcccc
     2161 cgttcgaggg cccgttcaca ggcccagacc acataggagg tgaaatcttc gtggccccag
     2221 gaacgcagtt gctccttcac cacgggattc acttccggtt tttcgccggt caggaacagc
     2281 agttccttgg cccgacgcct gaccgcttcg tccagcatcc gctccacctc ttcgggtgga
     2341 tgaacatggg cctgatgcgt ggcgaaagcg cagtacttgc agtagcactg gcaggtccgc
     2401 gagagcgaga tcgtgtaatt gcgcgaaaag gtgacccgac gcatccgttg tccaacctac
     2461 cgcagtgaac tttttgcgcc ggttcgccgt agtacccggt tagtgtgaaa caagcgcatt
     2521 gaatcgagac gaaggagaac cccgtgaaag cttccctcaa gttcctggtc gtgccgctcg
     2581 ccgtgggcgc actgttcttc gccggttgcg gtggcgatga ctcgtccgac gacaattcct
     2641 cgtccgacac ggcccccaca cagagccagc cggccgataa cagcatggga agcggccagg
     2701 ttctgaatct cgccgccgat ccgtccggtc agcttgccta cgacaagacc gagctttccg
     2761 ccaaggcggg caaggtcacc ctcgacttca ccaacgagtc tccgatcggc cacaacgtcg
     2821 tgatcgagga ttcgagtggc aaggaaatcg cctcgaccga cacgatcacc ggccagagca
     2881 ccaccgccga gttcaagatc aagcccggca cgtacacctt ctactgctcg attcccggtc
     2941 acgagcaggc tggcatgaag ggcactctca ccgtccagta agatcgccgc gagcgatggg
     3001 gctcgctcct tcataaccgc ggtcgttccg ctgatagctc ctgtcaaccg tcccccggga
     3061 ccgataggag ttgcatgcga atgatcttca ggcgtcaccc catccgcaaa ctcgcggcga
     3121 tctacgccgg cggcgtttgc ggcgccctgc tcagggtcag cctgggcgaa gcatttcccc
     3181 acggggccga gacctggccc tggccgacct tcgcggccaa cctgatcggg gccttcctga
     3241 tcggctactt cttcgcgtcc ttctccgatc acacgcccga tcaactccat caccccttct
     3301 tcgcgaccgg gatctgcggg agcctgacca ccttctcgac catgcagctg gagctcttca
     3361 acatggtcga tgcgcgcgaa tttctcctcg ctgcgaccta tacggcaacg acgctggcct
     3421 tcggctacgt ggcggtcaga ctcgggatcg ctgccgagag gtccggattg gaggtcggcc
     3481 cgtgaacgtc ttcggcgtcg gcatcctggg ctgggtgctg gtcggaatgc tgggcgggct
     3541 cgcagccggc gcccgttacc tgctcgacgc cgagatctcg cggctgaccg cctcgccgtt
     3601 cccgatcggg atattcgccg tgaacatgac cggggccttc ctgctcgggc tggtcgcagg
     3661 aagcacactt cacggcgagg ccctggtcat
//
LOCUS       DAOTIP010000106         3681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_29750_length_3681_cov_3.214837, whole genome shotgun sequence.
ACCESSION   DAOTIP010000106 DAOTIP010000000
VERSION     DAOTIP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29750_length_3681_cov_3.214837"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5138_02960"
     CDS             <1..388
                     /locus_tag="P5138_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59565.1"
                     /translation="FNERDFSQRVEQAAVRLGFVTPGLTFAELNDLLHLAVSGEIDEP
                     SSELGIHVTHHWEDLTGPGNQSFVHWLRRLVFRGAWLDQRVKEGELDIVFDEGRQSFG
                     YIQPERGPEMIELAKEPSWRRVAFRR"
     gene            complement(533..1894)
                     /locus_tag="P5138_02965"
     CDS             complement(533..1894)
                     /locus_tag="P5138_02965"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRV59566.1"
                     /translation="MSDLKSKSLAELHELAGKAGIEKFRLLRRDELIEKLDQGEGSDS
                     DWSGSDSQGKGSGSRRRRGDRGRRGDRERRSDRERRNGRDRDEEKEEKGGRDSEGDDS
                     EAEPELTEITGVLDVLPRGSGIVRSEDGPSAGIYVSPAQIRRCELRSGDEVSGPVRPA
                     RRGERRPSLVRVEKVNGEPPVDERSPRFESLTPIAPHRHMPLSGDTNDILARSVDLLA
                     PLAFGQRVLVEAQPRSGRTSFLRSLAKAIARGAGDGAEIIVLLIDERPEEVTAWSREV
                     PEVEIAAAPADLGAHEQVRTAERILAKVKRQAEAGNDVVIIVDSLTRLGNAYGEPGAI
                     KPFFGAGRELEEEGSGSVTVIGTALWGSPGDQDVMDAVHTTENATIRLDASLAAAGVE
                     PSLDVAACAVSGQESVLDEAEVDAVRRLRAELGAMEPVPAANRLAELIKGSSSNEELL
                     KKL"
     gene            complement(1896..2582)
                     /gene="fsa"
                     /locus_tag="P5138_02970"
     CDS             complement(1896..2582)
                     /gene="fsa"
                     /locus_tag="P5138_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRV59567.1"
                     /translation="MELFIDTGAVSEVEEIAAWGVLSGATTNPSLLAKEDGEPGDIIR
                     KIADLVNGPTSAEVVSPDAEGMIAEGQALRKLHEHVTVKVPFGPEGLKATKALTDDGA
                     MVNMTLVFSPNQALLAASAGATFVSCFMGRLDDISVDSAKVVEEICDVLRSRDLESKV
                     LAASIRHPEHMVVAASLGCDIATVPAKVFHQMLNHPLTTEGAAKFEEDWKSRPEFGEW
                     LSGLVAADKG"
     gene            complement(2687..>3681)
                     /locus_tag="P5138_02975"
     CDS             complement(2687..>3681)
                     /locus_tag="P5138_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV59568.1"
                     /translation="ASGETAVVEADNIVNATGVWADRIRPGEIEREEDVPKIAPSRGA
                     HILFSAEDVDMGKAACIVPAGEGRRIFALPWYGRTLVGTTDVDHHGGIEEVGPTGDDI
                     DYLMSAMNEFLDLSLTDSDIVGAYAGVRPLISTGDPRKSVDISRQAELYETSSGMLTI
                     TGGKLTTWRRMAKQTVDRIVERAGREARCQTAEIPLGMEIDPAELSAPEGVSKESLAQ
                     LAFRYGHAARGVVRLAEADPEMARPIVEGLPDLMAEVQIAIEFEQARSLADVLLRRTR
                     LGLTAASSLASAESVEAVATVMGRGLGWGDIEKKHQIDQWLETLRAEGLNPAGA"
BASE COUNT          581 a         1264 c         1194 g          642 t
ORIGIN      
        1 gttcaatgaa cgggacttca gccagagagt cgaacaggcc gcagtgcggc tcggcttcgt
       61 cacccccggc ctgaccttcg ccgaactcaa cgacctgctc catctcgccg tctccggcga
      121 aatcgacgag cccagttcag aactcgggat ccacgtgacc caccactggg aagacctgac
      181 cggccccggg aatcagagct tcgtccactg gctccggcgc ctcgtattcc gcggtgcctg
      241 gctcgaccag agggtcaagg aaggtgaact cgacatcgtc tttgacgagg gtcgccagag
      301 tttcggctac atccagcccg aacggggccc ggagatgatc gagctcgcca aggaaccttc
      361 ctggcgccgc gtcgccttcc gccgctaaag cacccgcaag tcgggcgggg tccgaccacc
      421 tgcaagtagg gccggtccga cccgaccttc aagttcaacc cgccccaccc ggacggtcat
      481 ccgcactgtc gtttacccac gatatttgtg ggcaaacgac agttcgaagg cttcaaagct
      541 tcttcaggag ctcctcgttg ctggacgagc ccttgatcag ctcggccagg cggttggcgg
      601 ccggcaccgg ctccatcgcg cccagctcgg cccgcaggcg acgcacggcg tccacctcgg
      661 cctcgtcgag caccgactcc tgaccggaaa ctgcacaggc ggcgacgtca agcgaaggct
      721 ccacaccggc cgcggccagg ctggcatcga gccggatggt cgcgttttcg gtcgtgtgga
      781 ccgcgtccat cacgtcctgg tccccggggg atccccagag cgcggtcccg atcacggtca
      841 ccgagccgga accttcctcc tccagctcgc ggcccgctcc gaagaacggc ttgatcgccc
      901 ccggttcgcc gtaggcgttt ccgagcctgg tcagggagtc gacgattatc acaacgtcat
      961 tgccggcctc ggcctggcgc ttgaccttcg cgaggatccg ctcggcggtc ctgacctgct
     1021 cgtgggcccc gaggtcggcg ggagctgcgg cgatctcgac ttccggcacc tcgcgcgacc
     1081 aggcggtcac ttcctccgga cgttcgtcga tcagcaggac gatgatctcg gcaccgtctc
     1141 cggcaccgcg ggcgatcgcc ttggcgaggg aacgcaggaa gctggtccgg ccggaacgcg
     1201 gctgggcctc gaccagaacc ctctgaccga aggccagcgg agcgagcaga tcgaccgaac
     1261 gggcgagaat gtcattggtg tcgccactga gcggcatgtg ccggtgaggt gcgatcgggg
     1321 tcaggctctc gaaacgcggt gaacgctcgt cgaccggcgg ctccccgttc accttctcga
     1381 cccggaccag cgaaggacgg cgctcaccgc gacgggcggg ccgtaccggt ccgcttacct
     1441 cgtctccgga gcgcagctcg caacgacgga tctgggccgg cgatacgtag atcccggccg
     1501 agggaccatc ctcggagcgg acgatgccgc tgccccgggg cagcacgtcg agtaccccgg
     1561 tgatctcggt cagttcgggc tctgcctcgg aatcgtctcc ttcggaatcc cgtccgccct
     1621 tttcttcctt ctcctcgtca cggtcacgac cgttccggcg ttcgcggtca ctgcgccgtt
     1681 cgcggtcacc acgacggcct cggtcgccgc gccgtcggcg cgaacccgag cccttgccct
     1741 gggaatcgct gccggaccag tcggaatcgg acccttcgcc ctggtcgagc ttctcgatca
     1801 gctcgtcccg acggagcagc cggaactttt caatgccagc cttgccggcg agctcgtgga
     1861 gctcggccag cgacttcgat ttgaggtctg acatgtcagc ctttgtcggc ggcgaccaga
     1921 ccgctcagcc actcgccgaa ctcggggcgg ctcttccagt cctcttcgaa cttggcggcg
     1981 ccctcggtgg tcagcgggtg attgagcatc tggtggaaga ccttggccgg aacggtcgcg
     2041 atgtcgcagc cgagcgaggc ggcgacaacc atgtgttccg gatgacggat cgaggcggca
     2101 agcaccttcg attcgaggtc acgcgaacgc agcacgtcac aaatctcttc gacgaccttg
     2161 gccgaatcga ccgagatgtc atcgaggcgt cccatgaagc aggagacgaa agtcgccccc
     2221 gccgaagcgg ccagcagggc ctggttcggc gagaagacga gagtcatgtt gaccatcgct
     2281 ccgtcatcgg tcagcgcctt ggtcgccttc aggccctcgg ggccgaaggg gaccttgacg
     2341 gtcacgtgct cgtggagttt gcgcagggcc tggccctcgg cgatcatgcc ctccgcatcg
     2401 ggagagacga cttcggcgga agtcgggccg ttgaccaggt ccgcgatctt gcggatgatg
     2461 tcaccgggtt caccgtcttc cttggcgagc agggagggat tggtggtcgc gccactgagc
     2521 acgccccagg cggcaatctc ctcgacctcg gaaacggcac cggtgtcaat gaaaagttcc
     2581 atgccacaag gatattgagt ctggtggcgg cggttccggt tgccatccgg tgaacgggtc
     2641 ccgaaccggc cattcggggc ccgggcgaac cgtcacccga gccggttcag gcgcccgcag
     2701 ggtttagacc ctcggccctg agggtctcga gccactggtc gatctgatgc ttcttttcga
     2761 tgtcgcccca accgaggccg cggcccatga ccgtggccac cgcttcgacc gattcggccg
     2821 aggcgaggct cgatgccgcg gtcagtccga ggcgggtccg gcgcagcagc acgtcggcca
     2881 gcgacctggc ctgctcgaac tcgatcgcga tctgaacctc cgccatcagg tcgggcagac
     2941 cctcgacgat cggcctggcc atttcgggat ccgcctcggc caggcggacc acgccacggg
     3001 cggcgtgacc gtacctgaac gcgagctggg cgagcgattc cttgctcacc ccttccgggg
     3061 cgctcagctc ggccgggtcg atctccatgc cgagcgggat ctccgcggtt tggcagcgcg
     3121 cctcgcggcc ggccctctcg acgatccggt cgacggtctg tttggccatc cggcgccagg
     3181 tggtgagttt gcctccggtg atcgtgagca ttccggagga ggtctcgtag agctccgcct
     3241 gacgcgagat gtcgaccgac ttgcgcgggt caccggtgct gatcagcggt cgcaccccgg
     3301 cgtaggcgcc gacgatgtcc gagtcggtca gcgacaggtc caggaactcg ttcatcgcgc
     3361 tcatcaggta gtcgatgtcg tccccggtcg gaccgacttc ctcgatgcct ccgtggtgat
     3421 cgacatcggt ggttccgacc agggtgcgcc cgtaccaggg gagcgcgaat atccggcggc
     3481 cctcgccggc cggaacgatg catgccgctt tgcccatgtc gacgtcctcg gccgagaaga
     3541 ggatgtgggc tccgcggctg ggggcgatct tcgggacgtc ctcctcccgc tcgatctcac
     3601 cggggcggat ccggtcggcc cagacgccgg tcgcgttgac gatgttgtcg gcctcgacca
     3661 cagcggtctc gcccgacgcg g
//
LOCUS       DAOTIP010000107         3661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_29966_length_3661_cov_3.171936, whole genome shotgun sequence.
ACCESSION   DAOTIP010000107 DAOTIP010000000
VERSION     DAOTIP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29966_length_3661_cov_3.171936"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="P5138_02980"
     CDS             complement(<1..512)
                     /locus_tag="P5138_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV59569.1"
                     /translation="MDASGSRPDEAARKRVAVELIRNHDRPLRWTARRFSICAEDAED
                     AYQRGIEILLSKAPTMDLRQLLPWTRTVIKHEALAIRKTRERTLARPGQSHEDEHLDE
                     DWVQLIPSAGDGPDDLTVRRERVARSKEALAQLKPAELKALTQLAEGFSYEEIGSMND
                     WTRTKVNRCLS"
     gene            829..1050
                     /locus_tag="P5138_02985"
     CDS             829..1050
                     /locus_tag="P5138_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59570.1"
                     /translation="MPSREEIQATIPRLGRGARVNLKLKDGGEVLGTLYGLFDDQVYF
                     EEEELGPIDLDRIEDLMVRIHTEQPGGPA"
     gene            complement(1167..1502)
                     /locus_tag="P5138_02990"
     CDS             complement(1167..1502)
                     /locus_tag="P5138_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59571.1"
                     /translation="MWIVTDRGFFSVVDKGEPEGCLCVRARVREDLEHLCRLESLTSY
                     ANSIEESELSDYRYRIHVKREDWVKAAADLAGRIDYDNFKNAVAARQGREREGYYSKV
                     WLTLLGMQR"
     gene            complement(1686..2201)
                     /locus_tag="P5138_02995"
     CDS             complement(1686..2201)
                     /locus_tag="P5138_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59572.1"
                     /translation="MGWFDDWEEVQTPEEEAADRQERRELLAYLLANSPREPRRFGIH
                     GFEVCQQCGEIGSYNGFWSAIGSHGGSGIRDKDHPIPPGAFIGGRCHDPSDTPPHPRR
                     RFCELCRDPVLLENADRVSYMFLCADCSSAIRKFGSGEELRTMIERFTRFRWRYHGLI
                     EEEPWDRGRYW"
     regulatory      complement(2208..2307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2357..2752)
                     /locus_tag="P5138_03000"
     CDS             complement(2357..2752)
                     /locus_tag="P5138_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRV59573.1"
                     /translation="MDSSVWIDHFNAVDSPEERALRDLIATEEPVGIVDLCLTEILRG
                     FRSEKDANLAMGFLISFPQLEVSGPGDHIQAAALYRRARKSGVTVKGSVDLLVASLCI
                     QHEARLLHKDRDFDQLATVSDLKVWKPTG"
     gene            complement(2758..2952)
                     /locus_tag="P5138_03005"
     CDS             complement(2758..2952)
                     /locus_tag="P5138_03005"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRV59574.1"
                     /translation="MRTNIVIDKELMADAQQLAGEKSMKETVDLALREFVNRRKRLGI
                     LELRGKLKWEGDLDEWRRAD"
     gene            3146..3376
                     /locus_tag="P5138_03010"
     CDS             3146..3376
                     /locus_tag="P5138_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59575.1"
                     /translation="MARTEGQIPDGLLTGMERQAAIRGVETDEMFESAVRSELAAYRN
                     REPGPLADLDELRLSFDWPASDSNGRRGRRHL"
     gene            complement(3437..>3661)
                     /locus_tag="P5138_03015"
     CDS             complement(3437..>3661)
                     /locus_tag="P5138_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59576.1"
                     /translation="DETPGGWQRFYAIRNTADEDEVITFGIFDGTLDELTASQSKDNK
                     YADRVAAVEPLVESTGTRGIFEVLVDQVID"
BASE COUNT          629 a         1149 c         1112 g          771 t
ORIGIN      
        1 gagagacacc gattcacctt ggttcgggtc cagtcgttca tcgacccgat ttcctcgtag
       61 gagaagcctt cagccagttg ggtcagagcc ttgagctcgg ccggtttgag ttgtgccagg
      121 gcttccttgc ttcgggcgac tcgctctcgc ctgaccgtca ggtcatcagg accgtcaccg
      181 gccgacggga ttagctgcac ccagtcttcg tcgaggtgct catcctcatg tgattggcca
      241 ggccgggcga gggttcgctc tctggtcttt cggatggcaa gcgcctcgtg cttgatcacc
      301 gtgcgggtcc aggggagtag ctgcctcagg tccatggtgg gtgccttgga gaggaggatc
      361 tcgatgccgc gttgatacgc atcctcggca tcctccgcgc agatcgaaaa ccgcctggcg
      421 gtccacctca gaggtcggtc gtgattcctg atcaactcga ccgcgacccg cttccgtgcc
      481 gcttcatccg gcctgctacc ggaggcgtcc atctccttgt cttgaaccgc aactcccctc
      541 atggggtccc ccacctctca gacggtctgg tctttgtcct atccgcaagc aactcgggat
      601 gccagtcgcc ggatcgggac caccgggagc catcccccac agtttcagcc cgttgaaaag
      661 tgtccatttg cagcgctaag ggaccggtcc gcgaagtctc tccccccttg cggaggtcgc
      721 ttttcccgca atttgcggag ttattggaaa tcaatgcaaa ttgcattgtt tttcttcaga
      781 aggcacttcc gaaatccgga aagtgggagg tccagctaag ttggaggcgt gccatcaagg
      841 gaagagatcc aggcgacgat cccccgcctc ggcaggggcg cccgggtcaa cctgaagctg
      901 aaggacgggg gcgaggtgct cggcaccctc tacgggctct tcgacgacca ggtctatttc
      961 gaggaagagg agctgggccc gatcgacttg gaccggatcg aggacctgat ggtcaggatc
     1021 cacaccgaac agccgggcgg accggcctag cgccgttcgc ggatcaggac gacgattgca
     1081 taaaccaggt tgatcagcag gaagggaccg aagctgtaag cgatcgcttc aaaggtggag
     1141 taggtgctgt ggctgtccac tgtcgcctac ctctgcatcc ctagaagcgt cagccagacc
     1201 tttgagtagt agccctcccg ctcgcggccc tggcgggctg cgacagcgtt cttgaagttg
     1261 tcgtagtcga tccgcccggc cagatctgcc gcggccttga cccagtcctc ccgcttcacg
     1321 tggatccggt agcggtagtc actgagctcg ctctcctcga tcgagttcgc atatgaggtc
     1381 agggactcga gccggcaaag gtgctccagg tcctcgcgaa cccgggcccg gacacacagg
     1441 caaccctcgg gctcgccctt gtccaccact gaaaagaaac cacggtcagt cactatccac
     1501 atcggcaccc tcctgggttg agttgcgagt gtcaggagag tcggtcttcc ccgggggttt
     1561 ctgacgccgg ccggtcaacc gttcgccgag ggctcggctg accgggaaga tgacgtggtg
     1621 gttcctttcg gttcctgccg agaggatcct gacgtgcttg taggtctttc ttccgcgacc
     1681 tttcatcacc aatacctccc ccggtcccag ggttcttcct cgatcaggcc gtgatagcgc
     1741 cagcggaatc ttgtgaatcg ctcgatcatt gtccgcagct cctcaccaga cccgaacttt
     1801 cgaattgcgc tcgagcaatc ggcacacagg aacatgtagg aaacccgatc agcgttctca
     1861 agcagaacag ggtctcgaca gagttcgcag aaccgccgcc tcgggtgggg cggggtatcg
     1921 ctcggatcgt ggcagcgacc gccgatgaat gcgccgggcg ggatcggatg gtccttgtct
     1981 ctgattccgc tgccgccgtg cgagccgatc gcactccaga atccgttgta gctgccgatc
     2041 tccccgcatt gctggcagac ctcgaagccg tggatcccga atcgccgggg ttcgcgcgga
     2101 gagttcgcca gcaggtaagc cagcaactcc cgtcgttctt gccgatccgc cgcctcttcc
     2161 tccggggtct ggacttcttc ccaatcgtcg aaccaaccca tgcttcacct ccatcggttc
     2221 cggcgttagc accgtttccg ggagatcact cccgttcggt tgctgcggcg tcaaagggcc
     2281 ggccccctca accgctcttg atagatgtct tgactctacc gtcggcgggt gggtcgaaat
     2341 ctgacggccg ggaagttcag ccggtcggtt tccagacctt caggtccgag acggttgcga
     2401 gctggtcgaa gtcacggtcc ttgtggagca gtcttgcctc gtgctgaatg caaagcgatg
     2461 cgaccagaag gtcgactgat ccctttacgg tcacgccgct tttccgggct cgccggtaaa
     2521 gggccgcggc ctggatgtga tctccgggtc cgctcacttc caattgggga aatgagatca
     2581 ggaaccccat tgcgagattc gcgtccttct ccgatcggaa ccctcggagg atctcagtca
     2641 ggcaaaggtc gacgatcccg actggttctt ccgttgcgat caggtctcgt agagcgcgct
     2701 cctccgggct gtcgaccgca ttgaagtggt cgatccagac actgctgtcc acgacgatca
     2761 atcggcccgg cgccattcgt ccaggtcgcc ctcccacttg agcttcccac gcagttccag
     2821 aatccccagg cgcttcctgc ggttgacgaa ttcccggagg gcgaggtcga ccgtttcctt
     2881 catgcttttc tcgccggcca gctgctgtgc gtcggccatc agttccttgt cgatgacgat
     2941 gttcgtcctc atacacatca cgatacacat tggccgtcct gtcctcaagt ggctggttca
     3001 ctcatggttg aagggaccgg aagggcaggt tcgccccctt gtcgggctgc cgtttcgggc
     3061 ggctgtttcc gcaaatgccg ggttttttgg ggtggtgagt tgacgtaact cacacagggc
     3121 agaggagcgg cgttatgttc ggctcatggc gaggacggag ggacagatcc cggacggact
     3181 gctcacagga atggagaggc aggccgcgat ccgcggggtc gagacggacg aaatgttcga
     3241 gtcggccgtg cgcagcgagc ttgccgccta ccggaaccgt gagcccggac cgctggctga
     3301 tctggatgaa cttcgcctga gcttcgactg gccggcgtcc gactcgaacg gacgccgcgg
     3361 gcggcggcat ctctgaagct ggccggcgcg cccctcgcgg ggcgcccgac ctggccgccg
     3421 gggatgccgc cgcaattcag tcgatcacct gatcgaccag cacttcgaag atgccgcggg
     3481 tcccggtcga ctcgacgagt ggttcaaccg cggccacccg atcggcgtac ttgttgtcct
     3541 ttgactggct cgcagtgagc tcgtccaggg ttccgtcgaa tattccgaag gtgatcacct
     3601 cgtcctcgtc ggccgtgttg cggatcgcgt agaaccgctg ccagccaccg ggggtctcgt
     3661 c
//
LOCUS       DAOTIP010000108         3641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30160_length_3641_cov_3.949805, whole genome shotgun sequence.
ACCESSION   DAOTIP010000108 DAOTIP010000000
VERSION     DAOTIP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30160_length_3641_cov_3.949805"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1345)
                     /gene="prfB"
                     /locus_tag="P5138_03020"
     CDS             complement(266..1345)
                     /gene="prfB"
                     /locus_tag="P5138_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157591830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRV59577.1"
                     /translation="MGTRLAAIDEHLALLTDYLDPDGLEAEVARLEDVMQQPGFWDDQ
                     DKAASVSAEHARAQRKLEGFRSLQSEAGDLSEMSEMAEADEEIAAELEEQLGSVESRL
                     EELEEARLFSGEYDHGDAIVSVHAGAGGTDSQDWAEILLRMYLRWAERRGFSVEMREA
                     SEGEEAGLKSATFLLKGENAYGLIAAERGVHRLVRISPFDSSSRRHTSFAQVDVAPVV
                     DEDVEIEIEDSDIRIDTYRASGAGGQHVNKTDSAVRITHEPTGIVVQCQNERSQTQNK
                     AVAMQMLKSKLVELEERKRAEVVNQERGEVKDVAWGSQIRSYVLQPYTMVKDHRTDFE
                     VGDAQRVLDGDIDGFIREYLNRTAG"
     gene            1418..2533
                     /locus_tag="P5138_03025"
     CDS             1418..2533
                     /locus_tag="P5138_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRV59578.1"
                     /translation="MPSTTSHIRNLTLVLGLAIVSLIGPASPAKASPDASASIINGRP
                     ASIGKWPWQVAVASSSSRKPGKSPWFRTFCGGSVVAPTVVLTASHCALEMVKGQVGDF
                     SVIAGRTNLNDAGAGQEVLISQIYFPLTNAGKIRYRTDQGWDVSLLRLAAPLAQEPVK
                     LLGPDEQDIVKPGRRLIETGWGTTITGRNFYPKSLRVGGTNIQPGSICRNQVGSRYFT
                     TTKQICLGDSRGKQANCYGDSGGPAVVATTEGYRQVGVTSFGTSDNCAGLIPNVDVLV
                     GGSPVRNWIRQGVQTLAGIDPVGSGGTAAPVHGLCHIPAVRGLTMRQARNRMRANGCS
                     RFRVKRLGRGNRISRLPALAGWLWDRKKPIRLVVGGR"
     gene            2555..3502
                     /locus_tag="P5138_03030"
     CDS             2555..3502
                     /locus_tag="P5138_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV59579.1"
                     /translation="MTATTGGVSDARRRRLIRVISVSIAVAVLTISMKFVAWLLTGSV
                     GLLSDAAESVVNLVAAGIALVVILWSTRPADEDHAYGHEKADYLSAGFEGALILLAAA
                     TIAYAAIERLIHPSELTEVGIGLAVTAAASVINLLTARLLIHTGREESSVVLVADGQH
                     LMADVVTSAGVIIGVGLVGLTGWETLDPIIALVVAANIVRTGVGLVSDSMSGLMDKAL
                     PEHELAEVSEVLDRYESRDIHFHALRTRKAGRRTFISIHVLVPGDWSVKEGHDLAEKV
                     EEDLRGRFDQATVFTHLEPIEDPVSFADTGLDRTTDPHS"
BASE COUNT          585 a         1223 c         1180 g          653 t
ORIGIN      
        1 aagcccgtcg cgcttcgcgc tcccccgaag gccttttccg ggtgtacccg cgccggacct
       61 gagagagcct ggattggccc ggcgcccggg ttgcgatggt ggcgggccgc agccattgag
      121 cgggtggatg ccaggagcct ccagggcggg gctgccgagg aataaggccg gaaatggccg
      181 cgatagtacg cgggtacgtg agctgccgtt tccggccgtt gacgaaggtc agcgccgtcc
      241 tggtggttcc tggcgccgcc cgggcctagc cggcggttcg gttgaggtac tcgcggataa
      301 atccgtcgat gtcgccgtcg aggacgcgct gggcgtcgcc gacttcgaag tcggtgcgat
      361 ggtccttgac catggtgtag ggctggagca cgtaggagcg gatctgtgat ccccaggcta
      421 cgtccttgac ctcgccccgc tcctggttca cgacttccgc ccgcttgcgt tcctcgagct
      481 ccaccagctt cgacttcagc atctgcatcg caaccgcctt gttctgggtc tgcgaacgtt
      541 cgttctggca ctgaaccacg atgccggtcg gctcatgcgt gatccggacc gccgagtcgg
      601 tcttgttgac gtgctggccg ccggcgcccg aggcgcggta ggtgtcaatc ctgatgtcgg
      661 agtcctcgat ctcgatctcg acgtcctcat cgacgaccgg cgcgacgtca acctgggcga
      721 agctggtgtg ccttcgcgaa gaggagtcaa agggcgagat ccggaccagg cgatgcacgc
      781 cacgttcggc ggcgatcagg ccataggcgt tctccccctt gagcaggaac gtggccgact
      841 tgagcccggc ttcttcgcct tcggacgctt ccctcatctc gaccgaaaag ccccggcgtt
      901 ccgcccagcg cagatacatg cggagcagga tctccgccca gtcctgcgaa tctgtccccc
      961 cggccccggc atgaaccgac acgatcgcgt caccgtggtc gtactcgccg ctgaagagcc
     1021 tggcttcctc cagctcttcc agccgtgact cgaccgatcc gagctgctct tcgagctcgg
     1081 cggcgatttc ctcgtccgct tcggccatct cggacatctc ggacaagtcc cccgcttcgg
     1141 actgaaggga gcggaaccct tcgagcttgc gctgggcgcg ggcatgttcc gccgaaacgg
     1201 aagcggcctt gtcctggtca tcccagaacc cgggctgctg catgacgtct tccagtctcg
     1261 cgacctcggc ctcgagcccg tcgggatcga ggtagtcggt gagcagggca aggtgctcgt
     1321 cgatcgctgc caggcgggtt ccgatcgact cggagttaag ggcttcagcc acaaatggag
     1381 cctacccgtc ccaatccctg cgaagattcc tcgggcgatg ccttcaacca ccagccacat
     1441 tcgcaatctg acgctggtcc ttggcctggc gatcgtttcc ctgatcggtc cggccagccc
     1501 cgccaaggcc tcgccggacg cttcggccag catcatcaac ggccgaccgg cctcgatcgg
     1561 aaaatggccc tggcaggtgg cggtcgcttc ctcatcctcc aggaagcccg gcaagagccc
     1621 ctggttccga actttctgcg gcgggtcggt ggtggcgccg accgtggtgc tgaccgccag
     1681 ccactgtgca ctggagatgg tcaagggcca ggtcggcgac ttctcggtca tcgccgggcg
     1741 cacgaacctg aacgacgccg gtgccggcca ggaagtgctg atctcgcaga tctactttcc
     1801 gttgaccaat gccggaaaga ttcgctaccg gaccgatcag ggctgggacg tctccctgct
     1861 ccggctcgcg gcacctctcg cccaggagcc ggtcaagctc ctcgggcccg acgagcagga
     1921 catcgtcaaa ccgggtcgca ggctgatcga gacgggctgg ggcaccacga tcaccggccg
     1981 gaacttctat ccgaaatcgc tgcgcgtggg tggcaccaac atccagcccg gatcgatctg
     2041 ccgcaaccag gtcggctccc ggtacttcac gaccacgaag cagatctgcc tcggtgactc
     2101 gaggggcaaa caggcgaact gttacggcga cagcggcggc ccggccgtcg tcgcaaccac
     2161 ggagggctac cgccaggtcg gtgtgaccag cttcggcacc tcggacaact gtgccgggct
     2221 gatcccgaat gttgacgtgc tggtcggggg cagtccggtt cgcaactgga tccggcaggg
     2281 cgttcagacc ctggccggaa tcgatccagt cggcagtggc ggtaccgctg cgcctgttca
     2341 tggcctgtgc cacatccccg ccgtcagggg cctgaccatg cggcaagcca gaaaccggat
     2401 gagggcgaac ggctgctccc gtttccgggt caagcggctc ggtcgcggca accggatttc
     2461 aaggctcccc gcgctcgccg gctggctctg ggaccgcaag aagccgatcc gcctcgtggt
     2521 gggtggccgc tagtccgcgg gagtggtttc gtccgtgacc gcaacaaccg gcggagtaag
     2581 cgacgctcgg cgcaggcgcc tgatcagggt catctcggtc tcgatcgccg tggcggtcct
     2641 gaccatcagc atgaagttcg tcgcctggct gctgaccgga tcggtcgggc tgctttcgga
     2701 cgcagcggag tccgtcgtga accttgtcgc ggccggcatt gctctcgtgg tgatcctctg
     2761 gtcgacccgg cccgccgacg aggaccacgc ctacggtcac gagaaagccg actacctttc
     2821 ggccggtttc gagggcgccc tgatcctcct ggcggcggcg accatcgcct acgcggcgat
     2881 cgagcgcctg atccacccat ccgagctgac cgaggtcggg atcggcctgg cggtcaccgc
     2941 ggccgcctcg gttatcaacc tgctgaccgc ccggctgctg atccacaccg gcagagagga
     3001 atcctcggtg gtgttggtcg ccgacggcca gcacctgatg gccgacgtcg tgacttccgc
     3061 cggcgtgatc atcggtgtcg gcctcgtcgg gctgaccggc tgggaaacgc tggacccgat
     3121 catcgccctg gtggtcgcgg caaacatcgt ccggaccgga gtcgggctgg tttcggattc
     3181 gatgtcgggc ctgatggaca aggccctgcc cgagcatgag ctcgcagagg taagcgaggt
     3241 cctggaccgc tacgagagcc gggacatcca ctttcacgcc ctgcggaccc gcaaggccgg
     3301 gcgtcgtacc ttcatttcga tccatgtgct ggtgccgggc gactggagtg tcaaggaggg
     3361 gcatgacctc gcggagaagg tcgaggaaga cctgcgcggc cgcttcgacc aggccaccgt
     3421 gttcacccat ctggaaccga tcgaagaccc ggtctccttc gccgacactg gcctcgacag
     3481 aaccacggac ccgcatagtt gaggcaaacc tagccacccg catttgcggc agggaccgcc
     3541 gtcggacccc attgggccgg tggtccggca cgggtacacc cgaaaaagat ccgtcgccct
     3601 gcgggctccc ccgaaggact ttttcgggtg tacccgcacc g
//
LOCUS       DAOTIP010000109         3640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30171_length_3640_cov_3.124128, whole genome shotgun sequence.
ACCESSION   DAOTIP010000109 DAOTIP010000000
VERSION     DAOTIP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30171_length_3640_cov_3.124128"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1423
                     /gene="murD"
                     /locus_tag="P5138_03035"
     CDS             56..1423
                     /gene="murD"
                     /locus_tag="P5138_03035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRV59580.1"
                     /translation="MEYVRSSKARPPLPEGPFLVVGLARSGIAAARALKARGEAVFGV
                     DSGEPEGLELLRQSGIDFETSVAGTDRLEGVATVIKSPGVPESAEVIAEALRRGVEVI
                     GELELGWRMFEAPVVAITGTNGKTTTTELTAHLFRAAGKPVSAAGNVGHPVCEVAMEA
                     VAGVEVGTVVCECSSFQLEDSTRFSPEVAVFLNLGPDHLDRHGNIESYREAKLRIFAN
                     QIEGDVAVLNADDPAVAGVRAKGATIRFSVEGAGKADLTLEDETIMIDGRPLIGISEM
                     RIIGRHNVANAMAGAASAIALGLDPDVVAAGLKSFPPVPHRYEPVAEIEGVVFINDSK
                     ATNVDATVAALESSDRQLHLILGGSTKGEPFDELTGPVAEHCRGIYLIGETADEIGKA
                     LEPAGLEIVRYCEDLEAAVSESAGAANPGEAVLLSPACASFDQFRNFEDRGDTFRRLV
                     RELDG"
     gene            1416..2786
                     /locus_tag="P5138_03040"
     CDS             1416..2786
                     /locus_tag="P5138_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRV59581.1"
                     /translation="MADRRRKKKPVRGKGSGSRGGSALRPAKVKGSGFSTEYSLLLTA
                     TMILLAFGVVMVFSASSTSQILSDGSLAGSTFYLKKTLIAVAIGLLCMWFVMRGSLSR
                     FREMTPKFMIGTLIALVLVLFMGAAINGTRGWFIFGPVQIQPAEFVKLGLVLYGANLL
                     ADRPDRLHSIRDMGPYLGMTGAALLLVLAQPDMGSAMVAVFAVFVTLFAAGARPRDLG
                     LLAGGAGMVAFVFALAAPYRRDRLLTFLNPDGDVTGNGFQIIQAKIAIGSGGFDGVGI
                     GNGVQKAFYLPEAHTDMISAVIGEEFGFLGMIALILVYGLLGYAGFQIARKAKDDYGR
                     ILAGGLTGLILIQAFINLYAVMGMAPLTGVPLPLVSYGNNSLIVSLISIGLILNVGRG
                     GKAATLSKPVPGRGPGGNARLRLIEGEGHRRQAKRTTGAERRDSGRRYGGARGSGPGR
                     SRRASR"
     gene            2746..>3640
                     /locus_tag="P5138_03045"
     CDS             2746..>3640
                     /locus_tag="P5138_03045"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRV59582.1"
                     /translation="MVPALAVADELRDRGVTVSFLGARGRLEAELVPGAGYEVDLLDL
                     SGIDRKNPVKAARAAAQAAAAIPRAGKLIRGRGADAVIGGGGFVAGPAGVAARRLKLP
                     LVLTEADRHLGLANRLLAKRANRVCLAFPIEGLEGPNVRITGRPVGRAVLTADRERAR
                     ERFGIAGDARCLLVMGGSQGARSINFAAIEALAERPGRDFDVIHVSGSRDHAEIQGRL
                     EEAPHGDRYTLLEYEPDMGDGIVASDLALARSGASVFELAALGRAAVLVPYPYATGDH
                     QTANADWMVEAGAAVVVSDADL"
BASE COUNT          597 a         1187 c         1242 g          614 t
ORIGIN      
        1 cagaagattg ccgctccacg gcagggaatt ggctttgggt ggggaagact cttgagtgga
       61 gtatgtccgc tcatcgaagg cccgcccgcc gctccccgaa gggcctttcc tcgtggtcgg
      121 gctggcacgc tccggaatcg ccgcggccag ggcactgaaa gcgcgtggtg aagcggtttt
      181 cggcgtggat tcgggcgagc ctgaaggact ggaacttctg cggcagtcgg ggattgactt
      241 cgagacctcg gtcgccggca ccgaccgtct cgaaggcgtt gccaccgtga tcaagagtcc
      301 gggggttccc gaatcggccg aggtgatcgc cgaagcgctc cggcgcggag tcgaagttat
      361 cggcgaactc gaactcggct ggcgcatgtt cgaggcgccg gtggtcgcga tcacgggcac
      421 caacggcaag accacaacga ccgaactgac cgcccatctc ttccgggccg cgggcaaacc
      481 ggtctccgct gccggcaacg tcggccaccc ggtttgcgag gtagcgatgg aggcagtcgc
      541 cggggtcgag gtcggaacgg tggtctgtga gtgctcgagc ttccagctcg aggattcgac
      601 ccggttctcc ccggaagtcg cggtctttct gaaccttggt cccgatcatc tggaccgcca
      661 cggcaacatc gagtcctacc gggaggcgaa gctgcggatc ttcgccaacc agatcgaggg
      721 tgacgtcgcg gttctcaacg cggacgaccc agccgtcgcc ggagtccggg ccaaaggcgc
      781 cacgatccgc ttcagcgtcg agggtgccgg caaggccgat ctgaccctgg aagacgagac
      841 gatcatgatc gacggccggc cgctgatcgg aatctcggaa atgcggatca tcggccgtca
      901 caacgtcgcc aacgcgatgg ccggggccgc ttcggcgatc gccctcggcc tcgatcccga
      961 cgtggtcgcg gccgggctga agagctttcc gccagttccc caccggtacg agccggtggc
     1021 agaaatcgag ggcgtcgtct tcatcaacga ctcgaaggcg accaacgtgg acgcgacggt
     1081 tgccgcgctc gagtcctccg accggcagct gcacctgatc ctgggcggca gcacaaaggg
     1141 tgagccgttc gacgagctga ccggcccggt ggccgaacac tgccggggga tctacctgat
     1201 cggtgagacc gccgacgaga tcggcaaggc ccttgagccg gccggcctcg agatcgtccg
     1261 ctattgcgag gatctcgagg cggcggtgag cgaatccgcc ggtgcggcga atccgggtga
     1321 ggcggttctg ctctccccgg cctgcgccag cttcgaccag ttcaggaatt tcgaagaccg
     1381 gggggacacc ttccggcgcc tggtcaggga actcgatggc tgaccggcgg cggaaaaaga
     1441 agccggtccg cgggaagggg tccgggtcca ggggtggcag cgccctgagg ccggccaagg
     1501 tcaaagggag cggtttctcg accgaataca gcctgctgct gaccgcgacc atgatcctgc
     1561 tggccttcgg ggtagtcatg gtgttctctg ccagctcgac cagccagatc ctcagtgacg
     1621 gaagcctggc cggcagcacc ttctatctga agaagaccct gatcgcggtg gcgatcggtc
     1681 tgctctgcat gtggttcgtg atgcggggca gcctcagccg cttccgtgag atgacgccaa
     1741 aattcatgat cgggaccctg atcgccctgg tgctggtgct cttcatgggg gcggcgatca
     1801 acggcacgag gggctggttc atcttcgggc cggtccagat ccagccggcc gaattcgtca
     1861 agctcggact cgttctctac ggagccaacc tgctggccga ccggcctgac cgtctccatt
     1921 cgatccgcga catgggcccc tacctgggca tgaccggcgc cgctctcctt ctggtcctgg
     1981 cccagcccga catgggctcc gcgatggtcg cggtctttgc ggtcttcgtc acgctcttcg
     2041 ccgcgggggc caggccgcgg gacctcggcc tgctggccgg cggggccggg atggtcgcat
     2101 tcgtctttgc cctcgccgcg ccctatcgcc gtgaccggct gctcaccttc ctcaatcccg
     2161 acggcgatgt caccggcaac ggattccaga tcatccaggc caagatcgcc atcggttccg
     2221 gcggattcga cggcgtagga atcggcaacg gcgtccagaa ggccttctat ctaccggagg
     2281 cccacacgga catgatctcc gccgtcatcg gcgaggaatt tggcttcctc gggatgatcg
     2341 ccctgatcct ggtctacggc ctgctcggat acgccggttt ccagatcgcc cgcaaggcga
     2401 aggacgacta cgggcgcatc ctggccggcg ggctgaccgg cctgattctg atccaggcct
     2461 tcatcaacct ttacgcggtg atgggcatgg ctcccctcac cggtgtgccg ctgccgctgg
     2521 tttcatacgg aaacaacagc ctgatcgtca gcctgatctc gatcggtctg atcctcaacg
     2581 tcggtcgtgg cgggaaggcc gcgaccctga gcaaaccggt tcccgggaga ggtccgggag
     2641 ggaatgcgag actgcgcctg atcgaaggtg aaggccacag aagacaagcg aaaaggacaa
     2701 ccggtgccga acgtcgtgat agcggccggc ggtacggcgg ggcacgtggt tccggccctg
     2761 gccgtagccg acgagcttcg agatagagga gtgaccgtgt ccttcctcgg cgcacgtggc
     2821 aggctcgagg cggagctcgt ccccggggcc ggatacgagg tcgacctgct cgatctctcc
     2881 ggcatcgatc gcaagaaccc cgtcaaggcc gcccgcgccg cagcccaggc cgctgccgcg
     2941 ataccccggg ccgggaagct gatccgcggg cgtggagccg atgctgtgat cggcggggga
     3001 gggtttgttg ccggtccggc cggagtggct gcccgccgcc tcaaactgcc actggtactg
     3061 accgaggcgg accgccacct cggcctcgcc aaccgtctgc tggccaaacg ggcgaatcgg
     3121 gtctgtctcg ccttccccat cgaaggcctg gagggaccga acgtgcggat caccggccgt
     3181 ccggtcggtc gcgcggtgct gacggccgac cgcgaacggg cccgcgaacg tttcgggatc
     3241 gccggggatg cccgctgcct gctggtcatg ggcggcagcc agggggcccg gtcgatcaac
     3301 ttcgcggcga tcgaagccct ggccgagcga cccggtcgcg acttcgacgt gatccacgtc
     3361 tccggctcga gggaccatgc cgagattcag ggccggctcg aggaggcgcc tcacggagac
     3421 cgctacacgc tgctcgaata cgaaccggac atgggggatg gcatcgtcgc aagcgatctg
     3481 gccctggccc gatccggggc gtcggtcttc gaactggcgg cgctcggccg ggccgcggtg
     3541 ctggttccct atccctacgc gaccggcgac caccagaccg cgaacgccga ctggatggtc
     3601 gaggccgggg ccgcggtcgt cgtgtccgac gccgatctcg
//
LOCUS       DAOTIP010000110         3640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30175_length_3640_cov_2.923013, whole genome shotgun sequence.
ACCESSION   DAOTIP010000110 DAOTIP010000000
VERSION     DAOTIP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30175_length_3640_cov_2.923013"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /gene="pgsA"
                     /locus_tag="P5138_03050"
     CDS             <1..541
                     /gene="pgsA"
                     /locus_tag="P5138_03050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRV59583.1"
                     /translation="LRILAVPVVVVALLDGTPNGDMLAAIVFALAALTDGLDGYIARS
                     RDSVTTFGKLMDPLADKLLITAALISLVSLNRLPAWVAMVIIAREFAITGLRSIAADR
                     GVVIAASWMGKVKTVLQIAAVFALIIFNPAPLWADILVYLALAATVISGFDYFFGLRK
                     RIEEQRAKEKTVAKQDPIP"
     gene            complement(560..1546)
                     /gene="mptA"
                     /locus_tag="P5138_03055"
     CDS             complement(560..1546)
                     /gene="mptA"
                     /locus_tag="P5138_03055"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HRV59584.1"
                     /translation="MNPEIALSPNAIDVQASQPATRVSLSRVGVTGVEKVVSIGEDGH
                     YHAELECYVDLAPDQAGVHMSRFEENVSEAIEEVVANESMRAEQLAARIAELVRVKQK
                     GLRAEVSINARYAQKMPAPVSGIENQEIYNLLGTAVASADVTRTVTGVQAQGMTACPC
                     AQEMVADLARGRLAEKGYTEDQVEEIIEEVPIATHNQRGIGTLWIGCTEGGPDLDARD
                     MLRLVESSMSSEIYELMKRPDEMSVVVKAHEQPRFVEDCVREMIRQVLETYPDLADDA
                     FVMAKQENLETIHRHNVVAERFGQIADLRQEVAGENPSRRHLTMKEWLELSA"
     gene            1666..2838
                     /locus_tag="P5138_03060"
     CDS             1666..2838
                     /locus_tag="P5138_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV59585.1"
                     /translation="MEFETDELKALAASGRDRGYLTFEAVAEAVSELDISRGQVEELN
                     GWLSEQGIELISREQSEVREGTGVATGSGLNGAPELEGSAGLEGAEPIEAEPIPTVTN
                     GAAASVGAGEAGSDATLDSLRLYLRSIGQVELLTAQQEVELAKRIERGDMEAKRQMVE
                     ANLRLVVSIAKGYLGRGLSFLDLIQEGSLGLIRAVEKFDYRRGYKFSTYATWWIRQAV
                     TRAVADKSRSIRIPVHMVEKLNRVHFVERQLVQDLGREPEPQEIAAELGWTVQEVRDV
                     WRVSQTPVSLEKPVGEGEETELVDLVKDENQMEPFDEASLNLRSEGVRRVLNSLPERE
                     REVLIMRYGLGGMNPRTLEECGEAFGVTRERVRQIETSTLRKIKSLPEAQGLREAG"
     gene            complement(2879..2960)
                     /locus_tag="P5138_03065"
     tRNA            complement(2879..2960)
                     /locus_tag="P5138_03065"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2924..2926),aa:Tyr,seq:gta)
     gene            3098..>3640
                     /locus_tag="P5138_03070"
     CDS             3098..>3640
                     /locus_tag="P5138_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRV59586.1"
                     /translation="MTDPVPRGFASDNCSGAHPAMLEAIAEANLGHVGSYGADPETER
                     FGELVRKEFGDRATGFPMLNGTGANVAVLRAMARPHQAVICASTAHISIDETAAPEAI
                     AGLKLLTVDTPDGKLTPELAATRLADPADIPHVAWPVVISVAQATEVGTVYTPEEITA
                     LADFAHENGMLLHVDGSRLAN"
BASE COUNT          627 a         1178 c         1199 g          636 t
ORIGIN      
        1 tctccgcatc ctcgcggtgc cggtggtcgt ggtggcgctg cttgacggca cccccaacgg
       61 cgacatgctg gcggcgatcg tcttcgccct ggccgccctg accgacgggc tggacggtta
      121 catcgcccgt tcgcgggatt cggtcacgac cttcggcaag ctgatggatc cgctggccga
      181 caagctgctg atcaccgccg cgctgatctc cctcgtttcg ctgaaccgtc tgcccgcctg
      241 ggtggcgatg gtgatcatcg cccgtgagtt cgcgatcacc ggcctccggt cgatcgccgc
      301 cgaccggggc gtcgtgatcg ccgccagctg gatggggaag gtgaagaccg tgcttcagat
      361 cgccgcggta ttcgccctga tcatcttcaa cccggccccg ctctgggccg acatcctcgt
      421 ctacctggcc ctggccgcca cggtaatcag cggcttcgac tacttcttcg gcctcagaaa
      481 gagaatcgaa gaacaaaggg ccaaagagaa aacagtggcc aaacaggacc cgatccccta
      541 gcggggtgcc ggctcccgcc taggccgaga gctcgagcca ctccttcatg gtgagatgcc
      601 gccggctcgg gttctccccg gcgacttcct gacggaggtc tgcgatctgt ccaaaccgct
      661 cggcgaccac gttgtgtcgg tggatcgttt cgaggttttc ctgcttcgcc atcacgaaag
      721 cgtcatcggc caggtcggga taggtctcga gcacctggcg gatcatctcc cgcacgcagt
      781 cctcgacgaa gcggggctgc tcatgggcct tgaccacgac cgacatctcg tcgggccgtt
      841 tcatcagttc gtagatttcg gagctcatcg agctctcgac cagacgcagc atgtcgcggg
      901 cgtcgaggtc gggtccgcct tcggtgcatc caatccagag ggttccgatt ccccgctggt
      961 tgtgggtcgc gatcggcacc tcctcgatga tctcctcgac ctggtcttcg gtgtatccct
     1021 tttcggccag acggccgcgg gcgaggtcgg cgaccatttc ctgggcgcag gggcaggcgg
     1081 tcatgccctg ggcttgaacg ccggtcacgg tgcgggtcac gtcggccgat gcgaccgcgg
     1141 tgccgagcag gttgtagatc tcctgatttt cgattccgga gaccggcgcc ggcatcttct
     1201 gggcgtagcg ggcgttgatc gagacctcgg cccggaggcc cttctgcttc acccggacca
     1261 gctcggcgat ccgggcggcg agctgctcgg cccgcatcga ctcgttggcc acaacctcct
     1321 cgatcgcctc ggagacgttc tcctcgaagc gggacatgtg aaccccggcc tggtcgggcg
     1381 ccaggtcgac gtaacactcg agttcggcgt ggtagtggcc gtcttcgccg atgctcacga
     1441 ctttctcgac tccggtcacg ccgacccggg aaagactgac cctggtggcg ggctgggagg
     1501 cctgaacatc gatggcattt ggggaaagtg cgatttcggg attcatcttg ctgtgaacag
     1561 cctagtaacc agcccgagtg gccggtgatt cacacttcgt ataattccgc aaccgcgcac
     1621 gaactcaggg tttccattag tgggaattct ttattggggg tagttatgga gttcgaaacc
     1681 gacgaattga aggctttggc cgcttcgggc agggaccgcg gctacctgac cttcgaggcg
     1741 gtcgcggagg cggtttccga gctcgatatc agtcgcggtc aggttgagga actcaacggc
     1801 tggctgagcg agcagggcat cgagctgatc agccgggagc aatcagaggt tcgggagggc
     1861 accggagtcg caaccgggtc cggcctgaac ggtgcaccgg aactcgaagg cagcgctgga
     1921 cttgaaggcg ccgaaccgat cgaggcggag ccgattccga ctgtgacgaa tggcgctgcg
     1981 gcatcggtcg gcgcgggaga ggccggctcc gacgccaccc tcgactcgct tcgcctctac
     2041 ctgcgttcga tcggccaggt cgaattgctc accgcgcagc aggaggtgga gctggccaag
     2101 cggatcgagc gcggcgacat ggaggcgaaa cgccagatgg tcgaggcgaa cctgcggctc
     2161 gtggtgtcga tcgccaaggg ctacctcggc cgcgggctca gtttcctcga cctgatccag
     2221 gaaggctcac tcggcctgat tcgcgccgtg gagaagttcg attaccggcg cggctacaag
     2281 ttttcgacct acgcgacctg gtggatccgc caggcggtga cccgcgcggt cgccgacaag
     2341 tcccgctcga tccggatccc ggtccacatg gtcgagaagc tgaaccgggt ccacttcgtc
     2401 gagcgccagc tggtccagga cctcggccgg gaaccggagc cgcaggagat cgcggccgag
     2461 ctcggctgga cggtgcagga ggtccgcgac gtgtggcggg tctcgcagac cccggtctcg
     2521 ctggagaaac cggttggcga gggcgaggag accgagctgg tcgacctggt caaggacgaa
     2581 aaccagatgg agcccttcga cgaggcctcg ctcaatctgc gcagcgaagg cgtgcgccgg
     2641 gtcctgaaca gcctgccgga acgggagcgc gaggtgctga tcatgcgcta cggcctcggc
     2701 ggcatgaacc cccgcaccct cgaggaatgc ggagaggcct tcggggtcac ccgcgaaagg
     2761 gtccgccaga tcgaaacctc gaccctccgc aagatcaagt ccctccccga ggcccagggc
     2821 ctccgcgaag ccggctgaaa cgcagaaagc cgccagcgct ttgccggcga cttccagatg
     2881 ggggagacag gattcgaacc tgtgtaggca gagccaactg gtttacagcc agtccccttt
     2941 aaccactcgg gcactccccc ggctgagcgg gacagtttat tgacccggcg aattcgacgg
     3001 gccgcgggcg gatcaaagcc ccgcgcgccg aggtgggtcg gatttccggc gagggcgccg
     3061 acaatccgac ccacctcggc agggcatact tcggggcatg accgatcctg ttccaagggg
     3121 gttcgcctcc gacaactgtt cgggggcaca tccggcgatg ctggaagcga tcgcggaagc
     3181 aaacctgggt cacgtcggct cctacggggc tgacccggaa accgaacgtt tcggggaact
     3241 ggtccggaag gaattcggag accgggcgac cggattcccg atgctcaacg gaaccggcgc
     3301 gaacgtggcc gtgctccggg cgatggcccg tccccatcag gcggtgatct gcgcttctac
     3361 cgcccacatc tcgatcgacg agacggccgc acccgaggcg atcgccggac tcaagctgct
     3421 gaccgtcgat accccggacg gcaagctcac cccggaactg gccgcaaccc ggctggccga
     3481 cccggccgac attccccatg tcgcctggcc ggtcgtgatc tcggtcgccc aggccaccga
     3541 ggtcggcacg gtctacaccc cggaggagat cacggccctg gccgacttcg cccatgagaa
     3601 cggcatgctg ctccacgtcg acgggtcgag gctggccaac
//
LOCUS       DAOTIP010000111         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30605_length_3600_cov_3.411283, whole genome shotgun sequence.
ACCESSION   DAOTIP010000111 DAOTIP010000000
VERSION     DAOTIP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30605_length_3600_cov_3.411283"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="P5138_03075"
     CDS             complement(<1..331)
                     /locus_tag="P5138_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV59587.1"
                     /translation="MSEMPEVPELSEMPEVPEFSTLKIEVEGPVGQMILNRPEKLNAL
                     SGVLLGEVILASGWFTERGDVKVVVVRGAGRAFSAGADLTAFTGAGPDPTAEERLEAA
                     DLGRRMAD"
     gene            361..801
                     /locus_tag="P5138_03080"
     CDS             361..801
                     /locus_tag="P5138_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV59588.1"
                     /translation="MIYLLRHGDAEPDQGSGDAARRLTDKGEAQALAAGLAMAKLGMN
                     VDTCLASPRVRALETANLACRSLGAEVEVVDAIGYDDYDSLDLAAGRGDTLIVGHEPD
                     MSMEVARLTGANIKMKKGGLAAIRGSQLQALLRPAELITIATQG"
     gene            complement(888..1901)
                     /locus_tag="P5138_03085"
     CDS             complement(888..1901)
                     /locus_tag="P5138_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HRV59589.1"
                     /translation="MAPTRKVFAALAGGLALALACSLMLATLAPGPASAATLEQKQAA
                     ARDRIQQTTDAIEADRADLDAAQQRADQASAREADLSGLLANGEQRSADLAEQLDAAE
                     AALARSKKRLARARRFLAEHLVAVYMSGGEPDTLDMALGAGSFGELASGTEYLTAIED
                     SNERLMERVTELRRQLNGEVGDLGDAKLAVDRHNAALAAARDQIASVRAAAESSAATL
                     ASENAARAGQIESLKGDIAGWQKQIEKQQKVSARQAEVEVAQNLGGPYSIPTYIVMCE
                     SGGNYSAVNPSSGAGGAYQIMPSTWEAYGGTGLPQEASKAEQDRIAALIWADSGPGAW
                     SCA"
     gene            complement(2005..2184)
                     /locus_tag="P5138_03090"
     CDS             complement(2005..2184)
                     /locus_tag="P5138_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59590.1"
                     /translation="MIYKLIGMAVVKLARFFLRRKAAANKTLFTAIGAAMAIALLATI
                     AGTVGYVLTRETPEA"
     gene            complement(2213..3484)
                     /locus_tag="P5138_03095"
     CDS             complement(2213..3484)
                     /locus_tag="P5138_03095"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRV59591.1"
                     /translation="MSEISLDSLSGPVASYEARPEEVNRVVLLYSGGLDTSVMLHWIQ
                     EAYDAEVVTLTVNLGQPGEDYGAILDKAKAMGAIETVVIDAREEFAEDFVLPAIKANA
                     LYGGGYPLFTSLGRPLIGKLGVEVAEKYGCDTLAHGCTGKGNDQVRLEGTVSTLNPEL
                     KMIAPVRAWQIGREEELEYCRQNGIPLKGGTSESPYSIDDNLWGRSSEGKIIEKLDTP
                     PPDDVFQLVTRPEEAPDEPEFVTIGFEAGRPVSFNGERLDLVPLLEAAADAGCRHGCG
                     IVDHIEDRIVGLKVRDIYEVPAAALVLTAHQDLEKVVSTIHQNNFKHSMDRHWAYMVY
                     AGLWYEPLRHDLDAFMDSANEFITGEVTLKLFKGSVTPVSRRSEYAVYDENLASFGAS
                     GGEFSQNAAPGFIELFSLQSRMAHQVRNRKD"
BASE COUNT          572 a         1207 c         1176 g          645 t
ORIGIN      
        1 cgtcggccat ccttcggccg agatcagccg cctccaggcg ctcctctgcg gtcggatcgg
       61 gcccggcccc cgtgaaggcg gtcaggtccg ccccggcgct gaaggcgcga ccggccccgc
      121 gcaccacgac gaccttcacg tcgccgcgtt cggtgaacca cccggaggcg aggatcactt
      181 cgcccagcaa cacgccggag agcgcgttca gtttctccgg ccggttgagg atcatttgac
      241 cgaccgggcc ttcgacttcg atcttcaggg tcgagaactc cggcacctct ggcatctcgc
      301 tcaactccgg cacctctggc atctcgctca tgacccgatc ctatggatag gctcgatccc
      361 atgatctatc tcctgcgcca cggcgatgcc gaacccgacc aggggtccgg tgacgcggcc
      421 cgccggctga ccgacaaggg cgaggcccag gctcttgctg ccggcctggc gatggcgaag
      481 cttggaatga acgtcgatac ctgcctcgcc tccccgaggg tgcgggcact cgaaaccgca
      541 aacctggcct gccgctcgct tggcgcagag gtcgaggtgg ttgatgcgat cggctacgac
      601 gactacgaca gcctcgacct cgccgccggc cggggcgaca cgctgatcgt cggccacgaa
      661 ccggacatgt cgatggaagt cgcccggctc accggcgcca acatcaagat gaaaaagggt
      721 ggcctcgccg ccatcagggg tagccaacta caggccctgc tacgaccagc ggaactcatc
      781 accattgcca cccaaggctg agcccctccg gaccatccgt tatttcgaat gggaggggcg
      841 ggttccgccg ggagggcggg tcggaccccg cccgacttgc agtcaggtca ggcgcaggac
      901 caggcgcccg ggcccgagtc ggcccagatc agggcggcga tgcgatcctg ttcggccttg
      961 gatgcctcct gcggcaggcc cgttccgcca taggcctccc aggtggaagg catgatctgg
     1021 taggcgccgc cggcgccgct ggaggggttg accgccgagt agttgccgcc cgattcgcac
     1081 atcacgatgt aggtcgggat cgaatagggg ccgccgaggt tctgtgccac ttccacctcg
     1141 gcctgccggg cgctgacctt ctgctgcttc tcgatctgct tctgccagcc ggcgatgtca
     1201 cccttgagtg attcgatctg ccccgcccgg gcggcattct cggaggcgag cgtggcggcg
     1261 ctcgattcgg ccgccgcgcg caccgaagcg atctggtccc gggcggccgc cagggccgcg
     1321 ttatgacggt cgaccgccag cttggcgtcc ccgaggtccc cgacctcgcc gttcagctgg
     1381 cgccgcaatt cggttacccg ctccatcagc cgttcgttcg agtcctcgat cgcggtcagg
     1441 tattcggttc cactggccag ttccccgaag ctgcccgcac cgagcgccat gtcaagggtg
     1501 tccggttcgc cgccgctcat gtagaccgcc accaggtgct cggccaggaa ccttcgggcc
     1561 cgggcgagtc gcttcttcga ccttgccagg gcggcctcgg ccgcgtcgag ctgctctgcc
     1621 agatcggccg agcgctgttc cccgttggcc agcaggccgg acaggtccgc ttcccgggcc
     1681 gaggcctgat cagcccgttg ctgtgccgcg tcgaggtcgg cgcggtcggc ctcgatcgcg
     1741 tcggtggtct gctggatgcg gtcgcgggca gccgcctgtt tctgctcgag ggtcgccgcc
     1801 gaagccggtc cgggggccag cgtggcgagc atcagcgagc aggcaagagc cagcgcaaga
     1861 ccgcccgcaa gggcggcgaa gactttacga gttggagcca aaatacgaag gattcaccgt
     1921 aacggtgacc cctgcgaacc ccggtgattc agctcacgga tgcacgatcg cttgcgccgg
     1981 gaccaacccg gctcttgccc ccgctcaggc ctcgggcgtc tcgcgggtga gcacgtaacc
     2041 caccgtgccc gcgatcgtgg ccagcaaggc gatcgccatg gcggcgccga tggcggtaaa
     2101 cagcgtcttg ttggctgctg cctttcggcg caggaagaac ctggcgagct tgacgacggc
     2161 cattccgatc agcttgtaga tcaccgggtt tcctccatgt cgtgaaccta ccctagtcct
     2221 tccggtttcg aacctgatga gccatccggc tctgtagcga gaagagttcg atgaaacccg
     2281 gggcagcgtt ctgggagaac tctcccccgg acgccccgaa ggaggcgagg ttctcgtcgt
     2341 agaccgcgta ctccgagcgg cgcgagacgg gggtgaccga gcccttgaac agcttcaggg
     2401 tcacctcgcc ggtgatgaat tcgttggcgg aatccatgaa ggcatcgaga tcgtgccgca
     2461 gcggctcgta ccagaggccg gcatagacca tgtaggccca gtggcggtcc atcgaatgct
     2521 tgaagttgtt ctggtgaatg gtcgaaacga ccttctccag atcctgatgg gcagtaagga
     2581 caagggctgc ggccggcacc tcgtagatgt ccctgacctt gaggccgacg atgcggtcct
     2641 cgatgtgatc gacgatgccg cagccatgtc tgcaacccgc atcggcggcc gcttccagca
     2701 gcggcaccag gtcgagccgc tcgccgttga agctgaccgg tcggccggct tcgaagccga
     2761 tagtcacgaa ctccggctca tcgggcgcct cttcgggccg ggtgaccagc tggaagacgt
     2821 catccggcgg gggcgtgtcc agcttctcga tgatcttgcc ttcggaggag cgaccccaga
     2881 ggttgtcgtc gatcgagtag ggagactcgg atgtgccgcc cttcagcggg atcccgttct
     2941 ggcggcagta ttcaagttcc tcctcgcggc cgatttgcca ggcccttacc ggggcgatca
     3001 tcttcagttc aggattcagg gtggagacgg tgccttcgag acggacctgg tcgttgccct
     3061 tcccggtgca gccgtgagcg agggtgtcgc agccgtattt ttcggccacc tcgaccccga
     3121 gcttgccgat cagcggtcgt cccagcgagg tgaacagcgg gtagccgcca ccgtagagcg
     3181 cgttcgcctt gatcgccggc agcacgaagt cctcggcaaa ttcctcgcgg gcatcgatca
     3241 cgacggtctc gatcgccccc attgccttcg ccttgtcgag gatcgctccg taatcctcgc
     3301 cgggctgacc gaggttgacg gtcagggtga ccacctcggc gtcgtaggcc tcctggatcc
     3361 agtggagcat cacgctcgtg tcgaggccgc ccgaatagag cagaacgaca cggtttactt
     3421 cctcggggcg ggcctcgtag gaggcgaccg gtcccgagag cgaatcgagt gagatttcgc
     3481 tcatccgggg ccgatcccgc cgctgctggt cccgctgccc gaatcggtgc ttgtgccgga
     3541 gtccggggtg acgccggcgt cgaccggtgg atgcgtcagg tcaccacgta cggcttctgc
//
LOCUS       DAOTIP010000112         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30672_length_3594_cov_3.604691, whole genome shotgun sequence.
ACCESSION   DAOTIP010000112 DAOTIP010000000
VERSION     DAOTIP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30672_length_3594_cov_3.604691"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5138_03100"
     CDS             <1..279
                     /locus_tag="P5138_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRV59592.1"
                     /translation="DEHDPVEKKMFGGVGFMVGGKMAIAASSRGGALVRVDPSESDHL
                     CELEGVERMEMRGRPMDGWLHVAPGRLDSDRDMQEWIDRGIAAAAAAS"
     gene            complement(276..1016)
                     /locus_tag="P5138_03105"
     CDS             complement(276..1016)
                     /locus_tag="P5138_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59593.1"
                     /translation="MKKFIAFLVAATAVIALAPQLASAKTGTLTGLYNTRYCEIFTVT
                     RPDPPNYQVDVYNTVGLNDCPADQWAAVDFDEVKAQAGSLAAVANGPRRWLIDTIAGG
                     KAGDPLTLGGLEVRHVGVLSVPSLSPDPYTELKIARTTTWVYNRGRKIHWIVSPQGRK
                     YALQAYTTNFDKTLRANNLDRMDKNPGMALPDGWKYRTIRLKKQLRLKAPGIATIMRD
                     GLGGTYQKFKWPKNFFKKPVKKKKTKRH"
     gene            complement(1088..1480)
                     /locus_tag="P5138_03110"
     CDS             complement(1088..1480)
                     /locus_tag="P5138_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV59594.1"
                     /translation="MIVVDASVLVPALAETGESGNLVRERIRGESLCGPELIDVEIAS
                     ALKRLERSGHLDSAVANGRISRLRHLPLERFPHWPLLERIWNLRHNLSTYDASYVALA
                     EMLGVPLVTADVSVGKAPGIRCEVDLIG"
     gene            complement(1477..1704)
                     /locus_tag="P5138_03115"
     CDS             complement(1477..1704)
                     /locus_tag="P5138_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59595.1"
                     /translation="MPSIQVKNVPEEVHRALQERARASGQSLQEFLQGELRRIADRAT
                     LDQIFKEAQREGGTFTFDEVIEAIHAERKGD"
     gene            1843..2496
                     /locus_tag="P5138_03120"
     CDS             1843..2496
                     /locus_tag="P5138_03120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRV59596.1"
                     /translation="MSIEPTRIDAQLAEYIRGHASGRDEALRLVEDETAAMGDVKIMQ
                     TAPEQAAFLEMLVRLGPGRRAIEVGTFTGYGAIRIARGLADDGSLLCCELDPERAAIS
                     RRNLDRAGVGEKVEIKVGPAIETIRSLPEEPAFDFAYVDADKTGYPDYYEELLRRMHP
                     GGLITFDNVFMGGRILDADPDEGTAAMQGLNDLLVDDDRVDSVMLGMADGLTLARMR"
     gene            complement(2525..3106)
                     /locus_tag="P5138_03125"
     CDS             complement(2525..3106)
                     /locus_tag="P5138_03125"
                     /inference="COORDINATES: protein motif:HMM:NF037009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRV59597.1"
                     /translation="MTQPQARTTRRDQVADAALRVLGESGSRALTHRAVDSAARVPQG
                     TTSNYFRTRSSLLEAAFARHAELDTPPEAALAGVADLELSDEEALDILMVVLDRLMDD
                     ASRGLLKARYELVLESSRDRDLLESFKSTREKFLALAEALVKARGCRTPSEHGKQLLV
                     VMDGALLDTVPGASTALDRDGIRDLLARQLKTC"
     gene            3264..>3594
                     /locus_tag="P5138_03130"
     CDS             3264..>3594
                     /locus_tag="P5138_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV59598.1"
                     /translation="MEAVRLAREKGLKLSVRSGGHSWAGNHLRDRTLLLDLSAMREFE
                     IDGAGMRASVQPGCPGNELDLELEKLDLFFPVGHCPGVALGGYLLQGGFGWNGRVHGP
                     ACQSVVAI"
BASE COUNT          615 a         1112 c         1220 g          647 t
ORIGIN      
        1 gacgaacacg atccggtcga gaagaagatg ttcggcgggg tcggcttcat ggtcggcgga
       61 aagatggcga tcgccgcttc gagccggggc ggagcactgg tccgggtgga cccttccgag
      121 tcagaccatc tatgtgagct cgagggcgta gagcggatgg agatgcgggg ccggccgatg
      181 gacggctggc tgcatgtcgc tcccggtcgg ctagattccg accgggacat gcaggagtgg
      241 atcgaccgcg gcatcgctgc cgcggccgcc gcgtcctagt ggcgcttggt ctttttcttc
      301 ttgacgggct tcttgaagaa gttcttcggc cacttgaact tctggtaggt gccgccgagt
      361 ccgtcgcgca tgatcgtggc gatgccgggt gccttcagcc gcagctgctt cttcagcctg
      421 atggtccggt atttccagcc gtcggggagg gccatgcccg ggttcttgtc catccggtcg
      481 agattattcg cccgcaaggt cttgtcgaag ttggtggtgt aggcctgcag cgcgtacttc
      541 ctgccttgcg gcgagacgat ccagtggatc ttccggcccc tgttgtagac ccaggtggtg
      601 gtccgggcta tcttcagctc cgtgtaggga tcgggggaaa ggctgggcac ggagagcaca
      661 ccgacgtgcc gaacttcgag gccgccgagg gtgagcgggt caccggcctt gccgccggcg
      721 atggtgtcga tcagccagcg acggggcccg ttcgcgacgg cggcgagcga tccggcctgg
      781 gccttgacct cgtcgaagtc gaccgcggcc cactggtcgg ccgggcagtc gttgaggccg
      841 accgtgttgt agacgtcaac ctggtagttc ggcgggtcgg gccgggtcac cgtaaagatc
      901 tcgcagtaac gcgtgttgta gagcccggtc agggtcccgg tcttggccga cgccagctgg
      961 ggcgccaacg cgatcacggc tgtggcagcc accagaaatg caatgaactt cttcaccttc
     1021 gtctccttcc ctcgacggac tccgaacata atagctgacg cacgtcaact tatgggaatc
     1081 gtcccgatca gccgatcaga tcgacttcac accggatgcc gggagccttt ccaaccgaga
     1141 catccgccgt caccagggga acgccaagca tttcggccag cgccacgtat gaagcgtcat
     1201 acgtggagag gttatgtcgc agattccaga tccgttccag aagcggccag tggggaaaac
     1261 gctcgagagg caagtgcctc aatctcgaga ttcgaccgtt cgccaccgca ctatccaggt
     1321 gccctgagcg ctcaagccgc ttgagggccg aagcaatttc cacgtcgatc agctcgggtc
     1381 cacaaaggga ctcgcccctg attcgctctc gaaccaggtt cccgctctca cccgtctcgg
     1441 ccagcgccgg tacaagtacg gaggcgtcaa cgacgatcaa tcgcccttcc tttcggcgtg
     1501 aatcgcctcg atcacctcgt cgaacgtgaa ggtgccacct tctcgctgcg cttccttgaa
     1561 tatctggtcg agtgtcgccc ggtcggcgat ccttcgaagt tcgccctgaa gaaactcctg
     1621 aagcgattgc ccggacgccc tggccctctc ctgaagagcg cggtgaactt cttccggcac
     1681 atttttgacc tggatcgacg gcatggtcgg agggtagcgc caaaatggcg ccacgccaaa
     1741 caccggtctg cacagacgaa ggattgccag gtttcataaa ggctcgaccg ccgggaactt
     1801 gccccttctg ggccgggtgt tccggcaggg taggtttggg gcatgtcaat cgaaccgacc
     1861 cgaatcgacg cacagttggc cgaatacatc cgcgggcacg cgagcgggcg cgacgaggcc
     1921 ctgcgcctgg tcgaggacga gaccgcggcg atgggtgacg tcaagatcat gcagacggct
     1981 cccgagcagg ccgcgttcct ggagatgctg gtccggctcg ggccaggccg gcgggcgatc
     2041 gaggtcggca ccttcaccgg ttacggggcg atccgcatcg cccgtggact ggccgatgac
     2101 ggctcgctgc tctgctgcga actcgacccc gagcgggccg cgatctcccg ccggaacctg
     2161 gaccgggccg gagtgggaga gaaggttgag atcaaggtcg gacccgcgat cgaaacgatc
     2221 cgctccctgc cggaggaacc ggccttcgac ttcgcctacg tcgacgccga caagaccggc
     2281 tatcccgatt actacgaaga gctgctcagg cgcatgcacc cgggcgggct gatcaccttc
     2341 gacaacgtct tcatgggcgg cagaatcctc gatgccgacc cggacgaggg cacggccgcg
     2401 atgcagggac tcaacgacct gctggtcgat gacgaccggg tcgactcggt gatgttgggc
     2461 atggccgacg gcctcaccct ggcccgcatg cgatagggcc ccggacccgc gcaggcggtc
     2521 gggatcagca ggtcttgagc tggcgggcca gcaggtcccg gatcccgtcg cgatccagtg
     2581 ccgttgacgc gccgggcacg gtgtccagca gggcgccgtc catcactacg agaagttgct
     2641 tgccgtgctc gctcggggtc ctgcagcccc gggctttgac cagggcctcg gccagagcga
     2701 ggaatttctc ccgggtggac ttgaacgatt cgagcagatc cctgtcgcga gaagactcga
     2761 gcaccagctc gtagcgggcc ttgagcaagc cgcgggaagc gtcgtccatc agtcggtcca
     2821 ggacgaccat caggatgtcc agtgcttcct cgtcgctcaa ttcgagatca gcgacgccgg
     2881 cgagggctgc ttcgggcgga gtgtccagtt cggcgtggcg ggcgaatgct gcctcgagca
     2941 gggaggagcg ggtgcgaaag tagttggagg tggttccctg gggaacacgg gcggccgaat
     3001 cgacggcccg gtgggtgagg gcacgggagc cactctcgcc gagtacgcgc aacgcggcgt
     3061 cggcgacctg gtcgcggcgg gtggtcctgg cttgaggttg agtcactaca ggtgtagtgt
     3121 accgggccat gggttttgac ggggaagttt tctcaagagg tgagtcaggt tacgagcaag
     3181 cccggcgggg agccgtctgg aacgcgcttg cccccgaccg gtttccggaa atcatcgtgt
     3241 gggcagccag cgagaccgat tgcgtggagg cggttcggct cgcccgggaa aagggcctga
     3301 agctgagcgt tcgctccggc ggacacagct gggccggcaa ccatctccgc gaccggaccc
     3361 tcctgctcga cctttccgcg atgcgcgaat tcgaaatcga cggggccggg atgcgggcct
     3421 cggtccagcc cggctgcccc ggcaacgagc tcgacctcga gctggagaag ctggacctct
     3481 tcttcccggt cggccactgc cccggcgtgg cgctgggcgg ctacctgctc cagggcggct
     3541 tcggctggaa cgggagggtc catggaccgg cctgccagag cgtcgtcgcg atcg
//
LOCUS       DAOTIP010000113         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30675_length_3594_cov_3.395592, whole genome shotgun sequence.
ACCESSION   DAOTIP010000113 DAOTIP010000000
VERSION     DAOTIP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30675_length_3594_cov_3.395592"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="P5138_03135"
     CDS             <1..1247
                     /locus_tag="P5138_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRV59599.1"
                     /translation="QVPDTLRVRPQGDIASQVIGAVSDEGEGLTGIEAGKNGVLKGVN
                     GERRLVKDALGDPIRLDNVRDAEDGKSVQLTLDATIQAEAEKILAEIGANSNPVNASA
                     IVMDARTSDVLAMANWPPVNLDDLGEADPKDLLNVGTSYTYEPGSTFKAITVASALEA
                     GTVTPTSTFTLAPSIQIYDRVIEEGHARGTVTLDVGDILAQSSNVGAVTIGLEMGGRK
                     FSQWIDNFGFGQPTGIDYPGEEQGIVLPYSDWSGSTIGNVPIGQGLAVTPIQMVQAYG
                     ALANGGILRTPRLIEKVDEQPVPEPKGRRIIKPEVADEVRDMLKGVLAPGGTASEVSV
                     PGYSLAGKTGTAQVATKNGYSDTRYVASFVGIAPADDPAIVVSIMVNEPEGAHTGGEV
                     AAPGFGELAAFTLPYLGVPTDQ"
     gene            1368..2822
                     /locus_tag="P5138_03140"
     CDS             1368..2822
                     /locus_tag="P5138_03140"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRV59600.1"
                     /translation="MRLDQLARDLASSELVGDPGTDIASLAFDNRKVAPGALFFCVRG
                     EKSDGHEFAPAAVEAGAAALVCERPLDLPVPQLIVADSRAAMAPLAATFNGEPTRDMK
                     MAGITGTNGKTTTAFLLRDILESAGVPSGLMGTVKQVIGGVEEEVVRTTPEAIDLQAA
                     FRRMLDAGDEACVMEVSSHAMVMHRADAIDFDLAVFTNLTQDHLDFHTDMEDYFQAKR
                     LLFAAGPGASVVNVDDEYGRRLASEFDCITYSATGSAADYSAVDVGFDAGGASFLVQS
                     EHGDLPVRTGLPGSFNVANALAALAGSISLGVDPDEAIAGLAEAGRVPGRFEPIDEGQ
                     DFAVLVDYAHTPDSVENVLRAARGLTEGRVISIVGAGGDRDRAKRPLMGRAGAELSDL
                     VVITSDNPRSEDPAAIIEDVLAGVDDGDAVLVEQDRDLAIGMAIREAGPGDTVVIAGK
                     GHEQGQEFENGRKIPFDDRDVARRHLRELTGKPA"
     gene            2819..>3594
                     /gene="murF"
                     /locus_tag="P5138_03145"
     CDS             2819..>3594
                     /gene="murF"
                     /locus_tag="P5138_03145"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRV59601.1"
                     /translation="MIELTTEQMAAAMGADVIARGEGGVPVRAEIDSRKLEGGELFFG
                     LTGGQSDGGAHASSAIEAGAWGVVVTPERAAALDGTDAFVFAAVDPLLSLQMLARAWR
                     RQLGATVIGITGSVGKTSVKDITRALLPGSVHASAENFNTEIGLPLTILEAPAGTETL
                     VLEMAMRGEGQIAELAVIAEPDIGVITNVGPVHVELLGSIEAVAAAKAELITNMKPEG
                     IVVVPFEAGYLDPHLKDVPGLIRFGGEGDVRVLELSVAAGA"
BASE COUNT          645 a         1149 c         1234 g          566 t
ORIGIN      
        1 gccaggttcc ggacaccctc agggtcaggc cacagggtga catcgcctcc caggtgatcg
       61 gtgcggtcag cgacgaaggg gaagggctga ccgggatcga ggcaggcaag aacggtgtgc
      121 tcaagggggt caacggcgaa aggcggctgg tcaaggacgc cctgggagac ccgatccggc
      181 ttgacaacgt ccgggacgcg gaggacggca agtccgttca gctgaccctg gacgcgacca
      241 tccaggccga agccgaaaag attctcgccg agatcggtgc caacagcaat ccggtcaatg
      301 cctcggcgat cgtgatggac gcgaggacct ccgacgttct cgccatggcc aactggccgc
      361 cggtcaatct cgacgatctc ggtgaagccg acccgaagga ccttctgaac gtcggcacct
      421 cctacaccta cgagcccgga tcgaccttca aggcgatcac cgtggcctcg gcgctggagg
      481 cgggcacggt gactcccacg agcaccttca ccctcgcccc gtcgattcag atctacgacc
      541 gtgtcatcga ggaaggccac gcgcggggga ccgtcactct tgacgtaggc gacatcctgg
      601 cccagtcatc gaacgtcgga gcggtgacca tcggactcga gatgggcggc cgcaagttca
      661 gccagtggat cgacaacttc ggcttcgggc agccgaccgg aatcgactat ccgggcgagg
      721 aacaggggat cgtccttccc tacagcgact ggtccggttc gacgatcggc aacgtgccga
      781 tcggccaggg gctcgcggtc accccgatcc agatggtcca ggcctacggc gctctggcca
      841 acgggggaat cctcagaacg cccaggctga tcgaaaaggt cgacgaacag ccggttcccg
      901 aaccgaaggg ccgccggatc atcaagccgg aagtcgccga cgaggtcagg gacatgctca
      961 agggcgtgct tgcccccggt ggtaccgcct ccgaggtttc ggtaccggga tacagcctcg
     1021 ccggaaagac gggtaccgcc caggtcgcga cgaagaacgg ctactcggac acccgttacg
     1081 tggcgtcctt cgtcggcatc gctccggccg acgatccggc catcgtcgtc tcgatcatgg
     1141 tcaacgagcc cgagggcgcc cacacgggtg gcgaggtcgc agcccccgga ttcggagagc
     1201 tggccgcctt cacgcttccc tacctcggcg ttccgaccga tcagtgagag ctggggctac
     1261 ctttgcccga gtcctttcct tctgtcggtg aatgcgagag gaagggacgg agttcgctgg
     1321 tcgggcgggt cggaccccgc cagacttcta gttctagaat catggccatg cggctggacc
     1381 aacttgcccg ggatctagcc tcgtccgaac tagtcggcga cccgggaacc gacatagcct
     1441 cgcttgcctt cgacaaccgg aaagtggctc cgggagcgct tttcttctgc gtcaggggag
     1501 agaagagcga cggccacgag ttcgcgcccg cggcggtgga ggccggggcg gcggcgctcg
     1561 tctgcgagcg cccgctcgac ctgccagtgc cgcaattgat cgtcgcggat tcccgcgcgg
     1621 caatggctcc ccttgccgcc accttcaacg gggaacccac ccgggacatg aaaatggccg
     1681 gaatcaccgg caccaacggc aagacgacca ccgccttcct cctgcgggac attctcgagt
     1741 ccgcgggcgt gccgagcggc ctgatgggaa ccgtgaagca ggtgatcggc ggggtcgagg
     1801 aggaagtggt ccggaccact ccggaagcga tcgacctgca ggccgccttc cggcggatgc
     1861 tcgacgccgg ggatgaagcc tgcgtgatgg aggtctcctc gcacgcgatg gtcatgcaca
     1921 gggcagacgc gatcgatttc gatctggcgg ttttcaccaa cctgacccag gaccacctcg
     1981 atttccacac ggacatggag gactacttcc aggccaagag gctgctcttc gcggcaggcc
     2041 ccggtgcctc cgtggtcaac gtcgacgacg aatacggacg ccggctggcg agcgagttcg
     2101 actgcatcac ctactcggct accggttcgg ccgcggacta ttcggcggtc gatgtcggct
     2161 tcgatgccgg aggagcctcc tttctcgtcc aatccgagca cggcgacctt ccggtgagga
     2221 ccgggctgcc cggaagcttc aacgtggcaa acgcgcttgc ggcgctggcg ggatctatat
     2281 ccctgggcgt tgatccggac gaagcgatcg ccggtcttgc cgaggccggt cgggtgccgg
     2341 gccggttcga accgatcgac gagggccagg acttcgcggt gctggtggac tacgcccata
     2401 cgccggactc ggtcgagaac gttctccggg cggctcgcgg gctgaccgag ggccgggtga
     2461 tctcgatcgt cggcgcgggg ggagacaggg accgtgccaa gcggccgctc atgggtcgcg
     2521 ccggcgccga actttccgat ctggtggtga tcacctccga caacccccga tcggaggatc
     2581 cggcagcgat catcgaggac gtgctcgccg gggtggacga tggtgacgcg gtgctggtcg
     2641 agcaggaccg ggacctcgcg atcggcatgg ccatacgcga ggccggtccc ggcgacaccg
     2701 tggtgatcgc cggaaagggt cacgagcagg gacaggaatt cgagaacggg aggaagattc
     2761 ccttcgacga tcgcgacgtg gctcgccggc atctgcgcga gctgaccggg aagcccgcgt
     2821 gatcgagttg acaacggaac agatggcggc cgcgatgggg gcggacgtga tcgcgcgggg
     2881 agaaggaggg gttccggtcc gggccgagat cgattcccgc aagctggagg gcggagagct
     2941 gttcttcggc ctcaccggag gccagagtga cggtggcgca cacgcttcat cggcgatcga
     3001 ggccggggcc tggggagtgg tggtgactcc cgaacgcgcg gccgccctcg atgggaccga
     3061 cgccttcgta ttcgccgcag tggatcccct cctttccctt cagatgctcg cccgggcctg
     3121 gcggcgtcag ctcggggcca cggttatcgg gatcaccgga tcggtcggaa agacctcggt
     3181 caaggacata acccgggcgc tcctgccagg ttcggtccac gcgtccgcgg agaacttcaa
     3241 cacggagatc ggcctgcccc tgacgatcct cgaggctccg gcggggaccg aaaccctggt
     3301 gctcgagatg gcgatgcgcg gggaaggcca gatcgccgaa ctggcggtca tcgccgagcc
     3361 ggacatcgga gtgatcacca acgtagggcc ggtgcacgtc gagctgctcg gctcgatcga
     3421 agccgtcgcg gccgccaagg ccgaactgat caccaacatg aagcccgaag ggatcgtcgt
     3481 ggtccccttc gaggcgggat acctcgaccc gcatctgaag gacgtgcccg gtctgatccg
     3541 cttcggcggc gaaggagacg tgcgggtcct ggaactctcg gtcgccgccg gcgc
//
LOCUS       DAOTIP010000114         3592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30705_length_3592_cov_3.115352, whole genome shotgun sequence.
ACCESSION   DAOTIP010000114 DAOTIP010000000
VERSION     DAOTIP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30705_length_3592_cov_3.115352"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5138_03150"
     CDS             complement(<1..215)
                     /locus_tag="P5138_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HRV59602.1"
                     /translation="MNWNEKDGVPWLEADLAGRARVVFSTRDGGGSEGPFRSLNLGIL
                     TDDDRGNVLENRLTLSAAVGFDPERVS"
     gene            complement(212..1330)
                     /locus_tag="P5138_03155"
     CDS             complement(212..1330)
                     /locus_tag="P5138_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59603.1"
                     /translation="MTPGIDPDFNRLAALIWTHPAIDRVRDSAEMPVYLVGGAIRDGL
                     ADFEVDDVDLVVEGDPVPLALSLDPEARVNERFGTVNLHVDGSPVDLATARTETYARP
                     GALPEVYPGTLEDDLIRRDFTINAMAMCVDRESELIDPFGGIADLRSGVLRVLHDRSF
                     EDDPTRALRAARYAARFGFDLEPKTAELMLGVNLGAVSSDRVENELRLIAGEDNSLEA
                     LRLARVWGLVDFDEERLDLAERAVELAEIEPWRGEAPREALILEAVFGALTGTVELLD
                     EPANPWEGYRSARGHSPLELLLARARGAEWLDQWQEKWRWVELEITGADLLAAGVPEG
                     PEIGTALDSALEAKLNGGLSGAEAELKVALEAAGVSRP"
     gene            1397..2779
                     /locus_tag="P5138_03160"
     CDS             1397..2779
                     /locus_tag="P5138_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRV59604.1"
                     /translation="MAAAPWYRQRNKIIALIAVVLVALGAIAFVAWSQLKRPDDVSNP
                     DVPFVVPDKKPKPKPKKKKTINWTTFRLNLQRTGYLPVNGIHPPFKRIWKYGDQPLLE
                     FPPIVVNGRLYFVDNDGTAHALRSDNGREIWHRKIASLNASTPTYSKGRLFIVNLEPG
                     HVLSMNAKNGKIIWEKPLPCRSESSPAVVHDRVYFGCENGVLYALKARNGKEVWETSV
                     SGAIKGAPAYQGGILYVGDYGGVMTAVRAKTGEIKWQSDGLGSSFGRAGSFYSTPAVA
                     YGRVYLGSNDGRVYSFDKNTGELAWTVSTGGWVYSGPTVAQVPGTPPSVYIGSFDGNV
                     YALNAKTGETRWTYSMGGRVIGSLSVIGRIVYAATFDGTTTYGLSAKNGHKVFEFHTG
                     AYMPAVSDGQKLYLVGYSSIHALKPITYKQLRAERRAKAARKAARIKARRQAAKAKAK
                     AKAQAGQNGQ"
     gene            2783..>3592
                     /locus_tag="P5138_03165"
     CDS             2783..>3592
                     /locus_tag="P5138_03165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV59605.1"
                     /translation="MDPGFEARLARRKDLVRRRQIVRRRILIGSFAGVLLCVAAFLLF
                     TRDSGNSGGPSSVPAASGNQDGNGGQNPAGSGNSENTARGGAHPDDSWETHTGPVPIL
                     MYHVIGPAAGAEDYPGLFLSTEDFRDQVNWLAQNGYTAVTLVQVQNAWYDGGTLPPKP
                     VVLSFDDGYLGQYLFAMPILEKQGWAGQLNLKSEGSDLSSKQVKKMYRAGWEIASHSI
                     THPDLTTLDPSALEHELVGSKQELQKDLGIRIINFCYPAGQYNDEVKAAVEA"
BASE COUNT          655 a         1240 c         1080 g          617 t
ORIGIN      
        1 atcgagaccc gctccgggtc gaagccgacc gccgccgaga gggtcagccg gttctcgagg
       61 acgttcccgc ggtcgtcgtc ggtgagtatc ccgagattca ggcttctgaa cggtccctcg
      121 ctcccgccgc cgtcccgggt gctgaagacg actctcgccc ggcccgccag gtccgcttcc
      181 agccacggca ctccgtcctt ttcgttccag ttcaaggtcg gctgactccg gccgcttcga
      241 gcgccacctt caattcggct tccgctccgc tgagtccccc gttgagtttg gcttccaatg
      301 ccgagtccag cgcggtgcca atctccggtc cctccggcac tcccgccgcg agaagatctg
      361 ctcccgtgat ttcgagctcg acccaccgcc acttctcctg ccattgatcg agccattcgg
      421 cgccacgggc cctggccaga agcaactcca gcgggctgtg cccacgggcc gagcggtaac
      481 cctcccaagg gtttgccggc tcgtccaaca gttcaaccgt gccggtcagg gcgccaaaca
      541 cagcctcgag aatcagggcc tccctcgggg cctcgccccg ccacggttcg atttccgcca
      601 gttccacagc ccgttcggcc aggtccaggc gctcttcatc gaaatctacg aggccccaca
      661 cccgtgccag tcgcagggcc tccagggaat tgtcttcccc ggcgatcagg cgcagttcgt
      721 tttctacccg gtcgctcgaa acggcgccca ggttcacgcc gagcattagt tcggccgtct
      781 tcggctcaag gtcgaatccg aaccttgccg cgtaccttgc cgccctcagg gcacgggtcg
      841 gatcgtcctc gaaggatctg tcgtgaagca cccgcaggac acctgaccgg agatcggcaa
      901 tgcccccgaa cgggtcgatc agctccgact ccctgtccac gcacatcgcc atcgcgttga
      961 tcgtgaagtc gcgacggatc aggtcgtctt cgagcgtccc gggatacacc tcgggcaggg
     1021 cgccgggccg ggcgtaggtt tccgtcctcg ccgtggccag gtctaccgga cttccgtcca
     1081 catgcaggtt cacggttccg aaccgttcgt tcacccgtgc ctccgggtca agcgaaagcg
     1141 cgagcgggac cgggtccccc tcgacgacga ggtcgacatc atcgacttcg aaatccgcga
     1201 gtccgtccct gattgctccc ccgaccaggt agacgggcat ttccgctgaa tcgcgcacac
     1261 ggtcgatcgc cgggtgggtc cagatcagtg ccgccagtcg gttgaagtca gggtcgattc
     1321 cgggagtcat cggtctactc ccgtaccatg atcccttctt cggatgaccg aaccaactga
     1381 caaccctcag gaaccggtgg cggctgcgcc ctggtaccgg cagcgaaaca agatcattgc
     1441 cttgatcgcc gtggtcctcg tggcactcgg ggcgatcgcc ttcgtcgcct ggagccaact
     1501 gaaacgcccg gatgacgtaa gcaatcccga cgtccccttc gtggtcccgg acaagaagcc
     1561 gaagccgaaa cccaagaaga agaagacgat caactggacc accttccggc tgaaccttca
     1621 gcgcaccggt tacctgcccg tcaacggaat tcacccgccc ttcaagcgaa tctggaagta
     1681 cggcgaccag ccgctgctgg agttcccgcc gatcgtcgta aacggccgcc tgtacttcgt
     1741 cgacaacgac ggcaccgcgc atgccttgcg cagtgacaac gggcgcgaga tctggcatcg
     1801 caagatcgcc tcgctcaacg cgtcgacccc gacctattcc aagggccggc tcttcatcgt
     1861 gaacctggaa cccgggcacg tgctctcgat gaacgcgaag aacgggaaga tcatctggga
     1921 gaagccactg ccctgccggt cggagtcctc gcccgccgtc gtccacgacc gggtctattt
     1981 cggttgcgag aacggcgtgc tctacgcgct caaggcgagg aacggaaagg aagtctggga
     2041 gacatcggtt tccggcgcga tcaagggggc gccggcctat cagggcggaa tcctttacgt
     2101 cggcgactac ggcggtgtga tgactgcggt ccgcgccaag accggcgaga tcaagtggca
     2161 gtcggacgga ctcggatcga gcttcggccg cgccggttcc ttctactcga ccccggccgt
     2221 cgcctatggc cgggtgtacc tcggcagcaa cgacgggcgg gtctacagct tcgacaagaa
     2281 caccggcgaa ctggcctgga ccgtctccac cggcggctgg gtctattccg ggcccacggt
     2341 cgcccaggtc ccgggcaccc cgcccagcgt gtacatcggt tccttcgacg gcaacgtgta
     2401 cgcgctaaac gcgaagaccg gcgagacccg ctggacctac tcgatgggcg gccgggtgat
     2461 cggctcgctc agcgtgatcg ggcggatcgt ctatgccgcg accttcgatg gcaccaccac
     2521 ctacgggctg agcgcgaaga acggtcacaa ggtcttcgag ttccacaccg gcgcctacat
     2581 gccggccgta tccgacggcc agaagctcta cctcgtcggc tattcgagca tccacgccct
     2641 gaagccgatc acctacaagc agctgcgggc cgagcgccgc gccaaggcag cccgcaaggc
     2701 agcccggatc aaggcccgca ggcaggcggc caaagccaag gcgaaagcga aggcccaggc
     2761 cggccagaac ggccagtagc cggtggatcc cggcttcgag gcccgactgg cccggcgaaa
     2821 ggacctggtc cgacgcaggc agatcgtccg ccggcgtatc ctgatcggct cgttcgcggg
     2881 agtcttgctc tgcgtcgcgg cttttcttct gttcacccgg gattccggca attcgggtgg
     2941 tccgtcaagt gtcccggcgg cgtccggcaa ccaggacggc aacggaggcc agaacccggc
     3001 gggaagcgga aattccgaaa acactgcccg cggcggagcc catcctgacg acagctggga
     3061 gacccatacc ggtccggttc cgatcctcat gtaccacgtg atcgggccag cggcgggcgc
     3121 cgaggactac cccggtctgt tcctgagcac cgaggatttc cgcgatcagg tgaactggct
     3181 ggcccagaac ggatacacgg cagtgacgct cgtccaggtt cagaacgcct ggtatgacgg
     3241 tggcaccctc ccgcccaagc cggtcgtcct ttcatttgat gacggctatc tcggccagta
     3301 cctcttcgcc atgccgattc tcgagaagca gggctgggct ggccagctca acctcaagtc
     3361 ggaaggctcc gacctctcct cgaagcaggt caagaagatg tacagagccg gatgggaaat
     3421 cgcctcccat tcgatcaccc atcccgatct caccacgctc gacccgtcgg cgctggagca
     3481 tgagctggtc ggctcgaagc aggagcttca gaaggacctg ggcatcagga tcatcaactt
     3541 ctgttacccg gccgggcagt acaacgacga agtgaaggcc gccgtcgagg cg
//
LOCUS       DAOTIP010000115         3590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30719_length_3590_cov_3.639321, whole genome shotgun sequence.
ACCESSION   DAOTIP010000115 DAOTIP010000000
VERSION     DAOTIP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30719_length_3590_cov_3.639321"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="aspS"
                     /locus_tag="P5138_03170"
     CDS             <1..1159
                     /gene="aspS"
                     /locus_tag="P5138_03170"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRV59606.1"
                     /translation="LLMMSGFERYFQIARCFRDEDLRADRQPDFTQLDIEMSFVTGED
                     VIEVNERLIRHVLAEMGVEVEIPFERMCYDEAIGRFGSDKPDFRYGYELADLTDILRG
                     TEFKAFGGVIEGGGQVKGINFGQRDLSRAQLDGLIDDAKELGAKGLVWAFREGDGWRA
                     PIAKFLSGQELRSLNEAMGAEEGDLMLVAADMPHMVAHILGGLRTRLAERWNLIPEDA
                     GNRLCWIVDWPLFEWNEDEDRWDALHHPFTAPKGDFDPENPGEARAEAYDLVWNGVEI
                     GGGSIRINKPGLQSEIFRTLGISDEEAGERFGFLLEALKYGAPPHGGIAYGVDRFAAL
                     LAGFESIRDVIAFPKTASGGDPLTGAPAPVDDIQLRDVGIELRKKKLPGQG"
     gene            1197..1484
                     /locus_tag="P5138_03175"
     CDS             1197..1484
                     /locus_tag="P5138_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59607.1"
                     /translation="MSLFDRFKEKVKSIGDGSDRIQIGDPRFHDWETVHQFEDLATGL
                     AWRDVLREHGIPAELTSDWELDRFNRGDIHLQVPPGMWSEAEELVSGLDLE"
     gene            1529..1681
                     /gene="ssrS"
                     /locus_tag="P5138_03180"
     ncRNA           1529..1681
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5138_03180"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1752..3551
                     /locus_tag="P5138_03185"
     CDS             1752..3551
                     /locus_tag="P5138_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HRV59608.1"
                     /translation="MSVPVIRMIRGGLAVLVTVAAALLLTASAQADTHRFYGEWGGSG
                     NYQASTKNGIGVDRNGNVYVATSAGLFGFSAEGVPQGLLVSNSYFVNGVEDVSVDGRG
                     WMYVVDTDQDMVFAFDSNGILQGQIGGNGAGNGLFNGPTGIAAGPDGEVYVSDTGNDL
                     IQVLDFQGAGFVRQWSTGLNGPTGIDVADDGTVYVADTDDDEIEKYQSDGSANVTLGS
                     TGLGAGQFIAPNDVSIGNDGNLYISEATDRIQVLEPNGTFVEEFGTTGSGQGEFDGNQ
                     ALAADRSGNVWVLDNGNNRVQIFCFSPRVIGGNSRDFGNVFIGNPIATQLIYMQNTNY
                     VLPMLVGPASLNTGTDFSLPAANRECENVILLPGHVCSIGVGFDPATAGLKSDTLNLD
                     FGWRQVSLSGTGVEGPTGPTGPTGDTGATGATGETGPTGPTGDTGATGETGPTGATGD
                     TGTTGDTGTTGDTGATGDTGPTGPTGPTGPTGEPGPGAPYIAKLIKGPVRFRAGVRVN
                     VARVTCQRAACSITGRRARIRVRGGKVARLRVLGPSSVAKGATARFGVRVPGRMVRRL
                     QRGRNSGVVMVYIGAKVNRGANGERNMRMGAMR"
BASE COUNT          667 a         1096 c         1223 g          604 t
ORIGIN      
        1 gctgctgatg atgtccggct tcgagcggta cttccagatc gcccgctgct tccgcgacga
       61 agacctcagg gccgaccgcc agcccgactt cacccagctc gacatcgaga tgagcttcgt
      121 caccggtgag gacgtgatcg aggtcaacga gcggctgatc aggcacgtgc tggcggaaat
      181 gggagtcgaa gtcgaaatcc ccttcgagcg catgtgttac gacgaagcga tcggacgttt
      241 cggctcggac aagccggact tccgatacgg ctacgagctc gccgacctga ccgacatcct
      301 ccggggcacg gaattcaagg ccttcggtgg tgtgatcgaa ggcggtggcc aggtcaaggg
      361 catcaacttc ggccagcggg acctttcaag ggcgcagctc gacggcctga tcgacgacgc
      421 caaggaacta ggggcgaagg gcctggtctg ggccttccgc gaaggcgacg gctggcgggc
      481 tccgatcgcc aagttcctct ccgggcagga gctcaggtct ctgaacgagg cgatgggggc
      541 cgaggagggc gacctgatgc tggtcgctgc cgatatgcct cacatggttg cccacatcct
      601 cggtggtctt cgcacccggc tggccgagcg ctggaacctg attccggagg atgccggcaa
      661 ccggctctgc tggatcgtcg actggccgct cttcgagtgg aatgaggacg aagaccgatg
      721 ggatgcgctt caccatccgt tcaccgcgcc gaagggcgac ttcgacccgg aaaatccggg
      781 cgaggccagg gcagaggcct acgacctggt ctggaacggg gtcgagatcg gtggcgggtc
      841 gatccgtatc aacaagcccg ggctccagtc cgagatcttc cggaccctcg gcatctccga
      901 cgaagaagcc ggggagcggt tcggtttcct gctcgaagcg ctgaagtacg gggccccgcc
      961 tcatggtggt attgcctacg gggtcgatcg cttcgcggcc cttctggccg gcttcgagtc
     1021 gatccgcgac gtgatcgcct tccccaagac agcctccggc ggtgacccgc ttaccggcgc
     1081 ccccgcgccg gttgacgaca tccagttgcg agacgtcggc atcgaactgc gcaagaagaa
     1141 actgcccggg cagggttgag ggaaggcccg gcgatccccg tgaacccgtt atcgccatga
     1201 gcctgttcga tcgcttcaag gagaaggtga agtcgatcgg ggacggctcg gaccggatcc
     1261 agataggcga cccgcgtttc cacgactggg aaacggttca ccagttcgag gatctggcca
     1321 ccggcctggc ctggcgggac gtcctgcgcg agcacggcat cccggccgag ctgacctcgg
     1381 actgggaact cgaccggttc aaccgtggtg acatccacct tcaggtgccg cccggcatgt
     1441 ggtcggaggc cgaggagctc gtctccggcc tcgacctcga atagccgcct ttgcggttta
     1501 ccggggcccg tagtggtagg ttggtttcga ggctcgaacc tgttcgtttg ccgtctcgta
     1561 aattgaccca acatcaacgg cacgggagct gatttcgatg tcccccgcgg gacgaagata
     1621 ggcacccacc tttttataaa gggatcaatg ccggaggcgg cgacggcagg ttggggcctc
     1681 cttttcatcc tggacttgca cgctggcgca accgccaata cggtgaagag gaactagcta
     1741 ggagggaatt catgtctgtt ccggtcatcc ggatgattcg cgggggtctc gccgttctgg
     1801 tcacggtcgc ggcggcactg ttactcacgg cgtctgcgca ggcagatacc caccgcttct
     1861 acggcgagtg gggtgggtcc ggcaactacc aggcctcgac caagaacggc atcggtgtcg
     1921 acaggaacgg caacgtgtat gtggcgacct cggccggcct gttcgggttc agcgccgaag
     1981 gcgtccccca gggacttctg gtttcgaaca gctacttcgt caacggggtc gaggacgttt
     2041 cggtcgacgg caggggatgg atgtatgtgg tcgacaccga tcaggacatg gtctttgcct
     2101 tcgactccaa cgggatcctg caggggcaga ttggtggaaa cggagccgga aacgggctct
     2161 tcaacggacc gaccggcatc gcggccgggc cggacggtga ggtctatgtg tccgacacgg
     2221 gcaacgacct gatccaggta ctcgatttcc agggagcggg cttcgtgagg cagtggtcga
     2281 ccggtctcaa cgggccaacc ggaatcgacg tcgccgacga cgggaccgtt tacgtagccg
     2341 acaccgacga cgacgagatc gagaagtacc agtccgatgg cagcgcaaac gtgaccctgg
     2401 gctcgaccgg actcggagcg ggtcagttca tcgcgccgaa cgacgtcagc atcggcaatg
     2461 acgggaacct ctacatatcc gaggcaacgg accggatcca ggtgctggag ccgaacggga
     2521 ccttcgtcga agagttcggt acgaccgggt ccggacaggg tgagttcgat ggcaaccagg
     2581 ctctcgccgc cgatcgctcg ggcaatgtct gggtgcttga caacggcaac aaccgggtcc
     2641 agatcttctg cttctcgccg agggtgattg gtggaaattc acgggacttc ggcaacgtgt
     2701 tcatcggcaa cccgattgcg acccagctga tctacatgca gaacacgaat tacgtcctgc
     2761 ccatgctcgt gggtccggcg agtctgaaca ccggaacgga tttctcactg ccggccgcga
     2821 accgcgaatg cgagaacgtg attttgctgc cagggcatgt ctgcagcatc ggggttggat
     2881 tcgacccggc caccgccggc ctcaagagcg acacgctgaa cctggacttc ggctggcgtc
     2941 aggtatcgct ctcgggaacc ggggtggagg ggccgaccgg tccgaccggg ccgaccggag
     3001 acaccggtgc gaccggcgca accggggaga cagggccgac gggcccgacc ggagacacgg
     3061 gagcgaccgg ggaaaccggt ccgaccggcg ccacaggtga cactggtacc acaggcgaca
     3121 ccggtaccac gggtgacacg ggcgcaaccg gagacaccgg tccgacgggt cccaccggtc
     3181 ccaccggccc gaccggtgag ccgggacccg gggccccata catcgcgaag ctgatcaagg
     3241 gtccggtccg gttccgggcc ggggtcaggg tgaacgtggc gcgggtgacc tgccagcggg
     3301 ccgcctgctc gataaccggg aggcgcgccc gtatccgtgt tcgtggcggc aaggtcgcgc
     3361 gactccgggt cctcggaccg tccagcgtcg ccaagggcgc aacagcccgc ttcggagtca
     3421 gggttccggg ccggatggtc cggcggctcc agcggggccg gaactcgggc gtcgtgatgg
     3481 tctacatcgg agccaaggtc aaccgcggcg ccaacggcga acggaacatg cgaatgggcg
     3541 cgatgcgctg attttcagga ggccgatgcg gccggggtga agtcgaaatc
//
LOCUS       DAOTIP010000116         3584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30789_length_3584_cov_2.830547, whole genome shotgun sequence.
ACCESSION   DAOTIP010000116 DAOTIP010000000
VERSION     DAOTIP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30789_length_3584_cov_2.830547"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="P5138_03190"
     CDS             <1..1380
                     /locus_tag="P5138_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV59609.1"
                     /translation="DLCERQHGLGDLGIGPGDGLAVMCRNHRGFVETNLAGWKLGANV
                     ILLNTMFSGPQLSDVIEREGAKAVVLDQEFEELLSGVPGGIGRVCGFEDEPGTSGLPS
                     LEDLIAGNDDGELPWPSEKGRNVILTSGTTGTPKGAQRPAPSGLSVMVGMLDRIPHFS
                     GETIVMAAPLFHSWGYLNSIIGTSVGARLVLQRRFNPARTMELVDEYDADALVAVPVM
                     LQRILELDAETSKHFKASKLGIVTLSGSALPGGLATAWMDRYGDNIYNFYGSTEVAMG
                     SIATPEDLRHDPATAGRPPYGTSVRLVDEFGDEVPHGETGRIFIRNEMTFDGYTGGGN
                     KENLDGYLSSGDVGHFDEDGRLYIDGRDDEMIISGGENVYPAEVEDLISGHDQVVESA
                     VIGVDDEKFGQALRAYVVLKTPDSLDEDGVRKYVKANLASYKAPRDVIFLPELPRNAT
                     GKILKKDLA"
     gene            1426..2586
                     /locus_tag="P5138_03195"
     CDS             1426..2586
                     /locus_tag="P5138_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59610.1"
                     /translation="MSDTTSAPSAEEAGSGNGLPPELRQVGEASAKLNSRIETLAKEL
                     SASESKAREAEIASREAELKALATARDSLTEARKAKAEAERDRAAAATADQRVEAVKA
                     QATRDVAEAREEIKTKARETLRKLSAEAREKIEASRKARLEAEEKAKELEQKDADQRE
                     AQARVEADLKRVEGTLKQERETRDEAMAKVAAEGEERLAARTRHLEEVAERLAKEARQ
                     AAEREAKNRIEAADERTRKAQERAAQAEAHANEFESLARQRIAEIEADVDRRVMEGTE
                     QVRREAAERVEELIERFGREAEENARRRADERLREEDERIQREADRRAELARQSAEDD
                     VQAAADRARQEALRAASSTAPEWERGDSGEGDGGPAPRRRRGGPGAGGYRTF"
     gene            2604..>3584
                     /gene="cysS"
                     /locus_tag="P5138_03200"
     CDS             2604..>3584
                     /gene="cysS"
                     /locus_tag="P5138_03200"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRV59611.1"
                     /translation="MREVRIRDTMSGELLTLGTDEEIGIYACGPTVYSRIHIGNARPF
                     VIFSLLARFLRSEGYGAKLAINVTDVNDKIYTAAAGRGVSSTELAAEMTAAYFEDTGR
                     LGIGRPDSEPLATETIGPIIDLIATLVEEGHAYESGGDVYFAVRSFDGYGKLSHRPTE
                     EMEQGEGDDAGSLKRDPLDFALWKAKKDGEDLSWESPWGPGRPGWHIECSAMAEAELG
                     QPFTIHGGGSDLVFPHHENEIAQSEAATGKEFVRNWTHNGLVTLGGEKMSKSTQHFFL
                     IEDLFARVDPATIRFYLLSTHYRSPIEFSEERLGEAGIAFGRLKATAQALR"
BASE COUNT          689 a         1186 c         1220 g          489 t
ORIGIN      
        1 gatctgtgcg agcgtcagca cgggttgggc gatctcggaa tcggccccgg cgacggtctg
       61 gccgtgatgt gccgaaacca ccgcggcttc gtcgagacca acctggccgg ctggaagctc
      121 ggtgccaacg tgatcctgct caacaccatg ttctcgggcc cgcaactttc cgacgtgatc
      181 gaacgcgaag gcgccaaagc cgtggtcctg gaccaggagt tcgaggagct gctctctggc
      241 gttccgggag gcatcggccg ggtctgcggg ttcgaggatg aaccgggcac ctcgggcctg
      301 cccagcctcg aggacctgat cgccgggaat gacgacggcg agctgccctg gccgagcgaa
      361 aagggtcgca acgtcatcct gacttccggc accacgggca ctcccaaagg cgcccagcgg
      421 cccgccccga gcggtctttc ggtgatggtc ggcatgctcg accggatccc gcacttctcc
      481 ggagagacga tcgtgatggc agcgcccctg tttcattcct ggggctatct caactcgatc
      541 atcggcactt cggtcggggc ccggctcgtc ctccagcggc gcttcaaccc ggcacggacg
      601 atggagctgg tggacgaata cgacgccgac gcgctggtcg cggtgccggt catgctccag
      661 cgcatcctcg aactggacgc cgagaccagc aagcacttca aggcgagcaa gctggggatc
      721 gtgacgcttt ccgggtcggc cctgccgggt ggtctggcga ccgcctggat ggatcgctac
      781 ggcgacaaca tctacaactt ctacggttcg accgaggtcg cgatgggttc gatcgccacc
      841 cccgaggatc tgcgccatga cccggccacg gcaggccggc cgccgtacgg gaccagcgtc
      901 cggctggtcg acgagttcgg cgacgaggtc cctcacgggg agaccgggcg gatcttcatc
      961 cgcaatgaaa tgaccttcga cggctacacc ggagggggca acaaggagaa ccttgacggc
     1021 tacctctcaa gcggcgacgt cggtcacttc gacgaagacg gccggctcta catcgacggc
     1081 cgcgacgacg agatgatcat ctccggcggg gagaacgtgt accccgccga ggtcgaagac
     1141 ctgatctccg gtcacgacca ggtggtcgag tcggccgtga tcggagtcga tgacgagaag
     1201 ttcggccagg cgctccgggc gtacgtggtg ctgaaaacgc ccgattcgct cgacgaagac
     1261 ggcgtccgca agtacgtgaa ggccaacctc gcctcctaca aggccccccg cgacgtgatc
     1321 ttcctgccgg aacttcccag aaacgccacg ggcaagatcc tgaaaaagga tcttgcctga
     1381 ccgcggatcg ggcgatccgt ggggttgcgt agtagttttt aggcgatgag cgataccact
     1441 tcagctccgt ctgcggaaga ggcaggctcc ggaaacgggc tgccccccga gctgcgccag
     1501 gtcggcgagg cgtcggccaa actcaactcg cggattgaaa cgctggccaa ggaactctcc
     1561 gccagcgagt caaaggcacg cgaggccgag atcgcttccc gtgaagccga actcaaggct
     1621 ctcgccaccg cccgcgactc cctgaccgaa gcccgcaagg ccaaggcgga agccgaacgg
     1681 gaccgggcgg ccgccgcgac cgccgaccaa cgggtcgaag cggtcaaggc ccaggccacc
     1741 cgtgacgtcg ccgaggcccg ggaagagatc aagaccaagg cccgcgaaac cctgcgaaag
     1801 ctgagcgccg aggcccgcga gaagatcgaa gcctcgcgca aggcacggct cgaggcggag
     1861 gagaaggcca aggagcttga gcagaaggat gccgaccagc gcgaagccca ggccagggtc
     1921 gaagccgacc tgaagcgcgt cgaaggcacg ctcaagcagg agcgcgaaac ccgcgacgag
     1981 gcgatggcaa aggttgctgc cgagggcgag gaacgcctcg cggcccgcac ccggcacctt
     2041 gaggaggtgg ccgagcggct ggccaaggaa gcccgccagg ccgccgagcg cgaagccaag
     2101 aaccggatcg aggccgccga cgagcggacc cgcaaggccc aggagagggc cgcccaggcc
     2161 gaagcgcatg cgaatgaatt cgagtcgctg gcccgccagc ggatcgccga gatcgaggcc
     2221 gacgttgacc gtcgcgtgat ggaaggcacc gagcaggttc gccgcgaggc ggccgagcgg
     2281 gtcgaggagc tgatcgagcg tttcggccgc gaagccgagg agaacgcacg gcgacgcgcc
     2341 gatgaacgcc tgcgcgaaga agacgagcgg atccagcgcg aggcggatcg ccgggccgag
     2401 ctggcccgcc agtcggccga ggatgacgtc caggccgcag ctgaccgtgc ccggcaggaa
     2461 gctctccgcg ccgcctccag caccgctccg gaatgggagc gtggcgattc cggcgaaggc
     2521 gacggcggac cggcaccaag gcgccggcgc ggcgggcccg gggccggcgg ctaccgcacc
     2581 ttctgagtag tctccccgcg gcaatgcgcg aggtcaggat cagggacacg atgagtgggg
     2641 aactcctcac tctcggcacc gatgaagaga tcggcatcta cgcctgcggc ccgaccgttt
     2701 acagccggat ccacatcggc aacgcccgtc ctttcgtgat cttctcgctg ctcgcccgtt
     2761 ttctccggag cgagggctac ggggcgaagc tggcgatcaa cgtcaccgac gtaaacgaca
     2821 agatctacac cgccgcggcc gggcgggggg tcagctcgac cgaactggcg gccgagatga
     2881 ccgccgccta tttcgaggac accggaaggc tcggcatcgg ccgccccgac tcggagcccc
     2941 tggcgaccga gacgatcggg ccgatcatcg acctgatcgc gaccctggtc gaggaaggcc
     3001 acgcctacga gtccggcggt gacgtctact tcgcggttcg gagcttcgat ggctacggca
     3061 agctctccca ccgtccgacc gaggaaatgg aacagggaga aggcgatgac gccggttcgc
     3121 tcaagcgcga cccgctcgat ttcgccctct ggaaggccaa aaaagacggc gaggacctga
     3181 gctgggaatc gccttggggc ccgggccggc cgggctggca catcgagtgc tcggcgatgg
     3241 ccgaggccga actgggccag ccgttcacga ttcacggagg cgggtccgac ctggtcttcc
     3301 cccatcacga gaacgagatc gcgcagtccg aggcggcgac cggcaaggag ttcgtccgca
     3361 actggaccca caacgggctc gtcaccttgg gcggcgagaa gatgtccaag tccacccagc
     3421 acttcttcct catcgaggac ctgttcgcgc gtgtcgaccc ggccaccatc cggttctacc
     3481 tcctctccac gcactaccgc agtccgatcg agttcagcga ggagcggctg ggagaggcgg
     3541 ggatcgcctt cggccggctc aaggcgacgg cccaggcgct ccgg
//
LOCUS       DAOTIP010000117         3574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_30931_length_3574_cov_3.285592, whole genome shotgun sequence.
ACCESSION   DAOTIP010000117 DAOTIP010000000
VERSION     DAOTIP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30931_length_3574_cov_3.285592"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..982)
                     /locus_tag="P5138_03205"
     CDS             complement(44..982)
                     /locus_tag="P5138_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59612.1"
                     /translation="MKRVPVLILAVAASLFGSISLAPVAQAGISCRYVAAGPDGPRGN
                     RLEIKLTRYEEAVALLPGEGSNIRVTDDQRMKTLRCKGGRPTMVNLDRVDFTADRGAT
                     GSSIYIAEAPTFGPGASPAGTGGKGITFIARGPALSFGIGGTTGPDFIQMGMAGKAAA
                     LDFYPDVFPGDGTGDGIDARIYAKFVNLLVRGDGGADSIAGSQIDYSLTSFTGPLTVP
                     TSIYGEGGPDALVGGRYMDYLDGGPGDDFLLGAAEADQLFGGPGIDQFRGGPGPDQID
                     AIDHNPGEDIRCGRGHDLARMDLKDVDRDCEAFRFP"
     gene            complement(1013..2560)
                     /gene="cobA"
                     /locus_tag="P5138_03210"
     CDS             complement(1013..2560)
                     /gene="cobA"
                     /locus_tag="P5138_03210"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRV59613.1"
                     /translation="MSGAGPGEVPQTADQRRAPVALVGAGPGDAGLMTRRSLELIADA
                     DVIYYDRLIPPEALEGAGPEAELVYVGKEPGKPGLGQEEINRRLVASGRAGLRVVRLK
                     GGDPFVFGRGSEEAEALQAAGLEFEVVPGVTAGVAASAAAGIPVTHRGVATAVTFSTG
                     HEDPDKPESTLELKRLAETPGTLVFYMGLKNLAENTSALIEGGRPSDQPAAVIEQGTT
                     SSQRVVAGTLATIAEQARAAGLKPPSLVVVGEVVSRREQLAWFEKRPLHGKSVVVTRA
                     RERVPALAGKLRLLGGEVVEIPVSRTEAVDPGEPHLSASLDRFGSGGFDLVCFTSPTG
                     VATFFERLAERALDSRAFAGAELAAIGPSTARALAERGLNADYLPERFVSEGMLKRLE
                     KVPMEGRRVLVIRAEEGRELLPDTLRERGAEVTVTPVYRTLPEQPLEEDLDRAAGADF
                     ITFTSGSSVHNFVRTGSVDPADFAPRVVTIGPVTSDAARGAGFTVSIEAERHDLDGLV
                     AAICSAV"
     gene            complement(2557..3450)
                     /gene="hemC"
                     /locus_tag="P5138_03215"
     CDS             complement(2557..3450)
                     /gene="hemC"
                     /locus_tag="P5138_03215"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRV59614.1"
                     /translation="MSSSGADSLTVASRPSPLALAQTALVRSALGLGEEAVLPVENES
                     GPADKERFVRGVEQALLTGRADLGVHSAKDLPGEMTAGLLIAAVPERADARDVWIGSG
                     GSVGEIPEGARVGTSSPRRRAQLMAIRPDLVMVDMAGNVDTRLARLQAGDADGLVLAM
                     AGLERLGRDSEAAFAFDLEQMVPAAGQGALVVQGRVNGTGLGQAAAINDESSFACVEA
                     ERAAVVAAGAGCDSPVGFHAVISGARMTIEGFAGRIDGSAWVRDRVEGDASRPAQLGR
                     ELAGRMLAAGAAGILADTVGT"
BASE COUNT          556 a         1290 c         1164 g          564 t
ORIGIN      
        1 gccgaagtcg gtgagaaagg tgaccggtct gggttctccg ggttcagggg aaacggaagg
       61 cctcgcaatc ccggtccacg tccttcaggt ccatgcgggc gaggtcatgg ccgcgaccgc
      121 agcggatgtc ctcgccgggg ttgtggtcga ttgcgtcgat ctggtccgga cccggtccgc
      181 cccggaattg atctatcccg ggaccgccga acaactggtc ggcttcggcg gctccgagca
      241 ggaagtcgtc gcccgggcct ccatcgagat agtccatgta acggccaccg accagtgcgt
      301 cggggccgcc ctcgccgtag atcgaagtcg ggacggtcag cgggccggtg aaactggtca
      361 acgagtagtc gatctggctg ccggcgatgg aatccgcgcc cccgtccccg cggaccagga
      421 ggttgacgaa cttcgcgtaa atcctggcgt ctattccatc tcctgtcccg tccccgggaa
      481 agacgtcagg gtagaaatcg agcgccgccg ccttgccggc catgcccatc tggatgaagt
      541 ccggcccggt tgtgcctccg atgccgaagc tcagcgcagg tcccctggcg atgaaggtga
      601 tgcccttgcc gccggtcccg gccggggatg ctcccggtcc gaaggtaggt gcctcggcaa
      661 tgtagatcga cgatcccgtg gccccgcggt cggccgtgaa gtcgacccga tcgaggttga
      721 ccatggtcgg cctgcctccc ttgcagcgga gagtcttcat ccgctggtca tcggtaaccc
      781 ggatgttcga gccctcgccg gggagcaggg cgacagcctc ctcgtaacgg gtcagcttga
      841 tctccagccg gtttccgcgc ggtccgtccg gcccggccgc gacgtaccgg cagctgatcc
      901 cggcctgggc gaccggagcc agggaaatcg agccaaacag ggatgccgcc acggcaagga
      961 tcaggacggg gacgcgcttc atgcgggaag tgtatttccc ggtaaccggg attcagacgg
     1021 cagagcagat cgccgcgacc agaccgtcga ggtcgtggcg ttcggcctcg atcgaaaccg
     1081 tgaacccggc cccgcgagcg gcatccgagg tgaccggtcc gatggtcaca accctcgggg
     1141 cgaaatcagc cgggtccacc gacccggtcc ggacgaagtt gtgaaccgag gagccggaag
     1201 tgaaggtgat gaagtcggcg ccggctgcac gatcgagatc ctcttccagc ggctgttcgg
     1261 gcagggtgcg gtagaccggc gtcaccgtga cctcggcccc gcgctcccgc agggtgtccg
     1321 gcaagagttc ccggccctcc tcggcccgga tgaccagcac ccggcggcct tccatcggga
     1381 ccttctcgag cctcttcagc atgccctccg agacgaaacg ctccggcagg taatccgcat
     1441 tcagtccgcg ctcagccagg gcccgtgcag tgctcggccc gatcgccgcc agttcggctc
     1501 cggcgaaagc gcgggaatcg agagcgcgct cggccagccg ctcgaagaag gtggccaccc
     1561 cggtcgggct ggtgaaacag accaggtcga aaccacccga accgaaccgg tcgagagacg
     1621 cggacagatg cggttcaccg gggtccacgg cctcggttct cgacaccggg atctccacca
     1681 cttcgccgcc cagcaggcgc agcttgccgg ccagggccgg gaccctctcg cgggcccggg
     1741 tcacgactac tgacttgcca tggaggggcc gtttctcgaa ccaggccagt tgctcgcgtc
     1801 tggagaccac ctcgccaacc acgaccaggg acggtggttt cagacctgct gctcttgcct
     1861 gctccgcgat cgtggccagg gtcccggcca ctacccgctg ggaggacgtg gtgccctgct
     1921 cgatcaccgc ggcaggttga tcggatggcc gtccgccctc gatcagggcg gaggtgtttt
     1981 ccgccaggtt tttcaggccc atgtagaaga cgagcgttcc aggcgtttcc gccagccgct
     2041 tcagctcgag ggtcgactcg ggcttgtccg gatcctcgtg cccggtcgag aaggtcacgg
     2101 cggtggccac gccccgatgc gtcacgggga tgcccgccgc cgcgctcgcc gcgaccccgg
     2161 cggtcacacc cggaaccact tcgaactcaa gtccggcggc ctgcagtgct tccgcttcct
     2221 cggagccccg gccgaacacg aagggatctc cgcctttcaa ccggacgacc ctcagcccgg
     2281 cccggccgct cgcgaccaga cggcggttga tctcctcctg gccgagtccg ggtttgcccg
     2341 gttcctttcc gacatagacc agctcggcct ccggaccggc tccttcgagc gcctccgggg
     2401 ggatcagccg gtcgtagtag atgacgtctg cgtcggcgat cagctccagc gacctgcggg
     2461 tcatcagccc cgcgtcaccc ggtccggcac cgacgagggc aaccggcgcc cggcgttgat
     2521 cagccgtctg ggggacctct cctggacctg ctccgctcac gtcccaaccg tatccgcgag
     2581 gatcccggcc gctccggccg cgagcatcct gccagctagc tcccgcccga gctgcgccgg
     2641 gcgcgaggcg tcaccttcga cccggtcgcg gacccaggcc gaaccgtcga tccgtccggc
     2701 aaagccctcg atcgtcatcc gcgccccgga gatcacggca tggaaaccaa ccgggctgtc
     2761 gcagcccgca ccggccgcga ccaccgcggc cctctcggct tccacgcagg cgaaggaaga
     2821 ctcgtcattg atcgcggcgg cctgcccgag cccggtcccg ttgacccggc cctggacgac
     2881 cagcgcgccc tgaccggcgg ccgggaccat ctgctccagg tcaaaggcga aggccgcctc
     2941 cgagtcgcga ccgagccgct cgagtcccgc catcgccagc acgaggccgt ccgcatcacc
     3001 tgcctggagc ctggccaggc gggtatccac attgccggcc atgtcgacca tgaccaggtc
     3061 cggcctgatc gccatgagct gggcccggcg ccgtgggctc gaggtgccga cccgggctcc
     3121 ttccgggatc tccccgaccg aaccgcccga cccgatccag acgtcccggg catcggccct
     3181 ttcgggcacc gcggcgatga gaagaccggc ggtcatttcg cccggaaggt ccttggccga
     3241 atgaaccccg aggtcggccc ggcccgtcag cagtgcctgc tcgacccccc ggacgaacct
     3301 ctccttgtcg gccgggccgc tttcgttttc gacgggcagg accgcctcct cgcccagtcc
     3361 cagtgccgac ctgaccaggg cggtctgcgc cagcgccagc ggactcggac gggaagcgac
     3421 ggtgaggctg tcggcacctg aagagctcaa ccctcttcgc gccggatcgg tgtcaccgtt
     3481 gcctcggagc cgccaccggg atccgtctct gtgttgagcc cgaacagctc ctgcagggtg
     3541 ttcaccgcct cgtaggccgc ctcggttccg gcca
//
LOCUS       DAOTIP010000118         3561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31061_length_3561_cov_3.503993, whole genome shotgun sequence.
ACCESSION   DAOTIP010000118 DAOTIP010000000
VERSION     DAOTIP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31061_length_3561_cov_3.503993"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="P5138_03220"
     CDS             <1..295
                     /locus_tag="P5138_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59615.1"
                     /translation="PAGALGTGNRGTRVQGPNLDQRTETYESALHAIQNGGFSGAIMP
                     QNIVVGEEAEKVATFVADYAGSDAGEAGADAYSQETDASVPEDAKPTTGTHGE"
     gene            311..1576
                     /gene="serS"
                     /locus_tag="P5138_03225"
     CDS             311..1576
                     /gene="serS"
                     /locus_tag="P5138_03225"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRV59616.1"
                     /translation="MLDLKLIRSEPERVKAALARRGAAEQVDRILALDERRRQLLPEI
                     EGARSQRKQESEKIGALKKAGEDATAQMEAVRQLKLDLEQMEGELESVESELGEVSAT
                     LPNLPDPTSPDGYTDEDAELVREVGEIPEFGFETRDHVELAGDSIELEAAAKVSGARF
                     AYLKGDLVMLEFALVRWALETLRGEGHEPVVPPILVREEALFGTGFLPGDRDQIYELE
                     KDELYLAGTSEVPLAGLHAGQILEADELPLRYAGFSSCFRREAGAAGRDTHGIFRVHQ
                     FDKVEMFSFVRPEDASAEHERILGIEEGILSALGLPYRVVNVAAGDLGGSAAKKYDCE
                     AWIPSQERYRELTSCSNTTDFQARRIGCRYRPEPGAPTEFVNTLNGTAIAVGRTIIAL
                     LENHQQADGSVRVPEILVAYGAPATIGPT"
     gene            1573..2229
                     /locus_tag="P5138_03230"
     CDS             1573..2229
                     /locus_tag="P5138_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV59617.1"
                     /translation="MSDHRVLICDDHWIVRQAIRQRISELPGFEVVDEVAQGKEVKPA
                     VREHRPDLVILDIEMPDVNGIQSMERILSRFPETRVLVFTAHDDPNLVRLAAREGADG
                     FLVKSAALDQIRDACETVMSGKKWFPGYSRKQLEEDDELKRFLSISEREREILALLAT
                     GMRAQGVAEEIGIRPATVYTHVRNVVGKLGVETRTQAVAIATRYSFLDPDPELRLTSR
                     "
     gene            complement(2233..2616)
                     /gene="msrB"
                     /locus_tag="P5138_03235"
     CDS             complement(2233..2616)
                     /gene="msrB"
                     /locus_tag="P5138_03235"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRV59618.1"
                     /translation="MKKTDSEWREELSPEAYEVTRKAATERPFTGPYLDHKGSGSYGC
                     VCCGKELFSSDAKFDSGTGWPSFTEPANLEHIELRQDISHGMVRTEVVCLNCDAHLGH
                     VFPDGPGPTGQRYCINGCALEFKEK"
     gene            2655..3245
                     /locus_tag="P5138_03240"
     CDS             2655..3245
                     /locus_tag="P5138_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRV59619.1"
                     /translation="MPALRVAKRSTPLACLALLLAAVLPVISAGPLGPGDAAAAGLIA
                     PAKYCSDNAANGGRAEMAKARRSMICMVNYARRRKGMRPYHRQPKLNWSSGRKARDIL
                     RCGFSHGACGRAFDYWIRRSGYLGGGGWATGENIAWGSGSLGSVRSIFVAWMKSKGHR
                     EAILDRSFTDVGVGVTRGRFSGIAGARIWVLHFGYN"
     gene            3389..3541
                     /locus_tag="P5138_03245"
     CDS             3389..3541
                     /locus_tag="P5138_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59620.1"
                     /translation="MSDQSELDVPLKTEQTFPPPEEFAAASEFSDPEIYARADADPEG
                     WWAGWA"
BASE COUNT          653 a         1157 c         1170 g          581 t
ORIGIN      
        1 cccggccgga gcgctgggca caggcaaccg cggtacccga gtccagggcc cgaacctgga
       61 ccagcgcacc gagacctacg agtcggccct ccacgcaatc cagaacggtg gtttctccgg
      121 cgcgatcatg ccgcagaaca tcgtggtcgg agaggaagcc gagaaggtgg ccacgttcgt
      181 cgccgactat gccggttccg acgccggtga agccggagcc gacgcttact cgcaggaaac
      241 ggacgcttcc gtccccgagg acgcgaagcc cacgaccggc acccacggcg agtaaccggc
      301 ggcgggggcc gtgctagacc tcaaactgat ccgctccgag cccgagcggg tcaaggctgc
      361 gctggcgcgc cgcggcgcgg cggaacaggt cgaccggatc ctcgctctcg acgagcgtcg
      421 acggcagctg ctgccggaga tcgaaggcgc ccgcagccaa cgcaagcagg agtcggagaa
      481 gatcggtgcc ctgaagaagg ccggcgagga cgcgaccgcg cagatggaag ccgtgaggca
      541 gctcaagctc gatcttgaac agatggaagg cgagctcgag tcggtcgagt ccgaactggg
      601 agaggtgtcg gcaacccttc ccaacctgcc cgacccgacc agccccgacg gatacaccga
      661 cgaggacgcg gagctcgtcc gcgaagtcgg tgagatcccc gagttcgggt tcgaaaccag
      721 ggatcacgtc gaactggccg gggattcgat cgaactcgaa gcggccgcga aggtgtctgg
      781 cgcccggttc gcctacctga agggcgacct ggtgatgctc gaattcgccc tggtgcgctg
      841 ggcgctggag actctgcggg gcgaggggca cgagccggtc gtgccgccga tcctggttcg
      901 cgaagaagcc ctcttcggca ccggcttcct gcccggtgac cgtgaccaga tctacgaact
      961 ggaaaaagac gagctctacc tggcgggcac ttcggaagtt ccccttgcgg gcctccatgc
     1021 cggccagata ctggaagcgg atgagctccc gctccgatac gcgggcttct ccagctgctt
     1081 tcgacgggag gcgggagcgg ccgggcgaga cacccacgga atcttccgtg tgcaccagtt
     1141 cgacaaggtc gagatgttct cgttcgtcag gcccgaagac gcttccgccg agcacgaacg
     1201 aatcctcggc atcgaggagg ggatcctgag cgcgctcggc cttccctacc gggtggtgaa
     1261 cgtcgccgcg ggcgatctgg gcggctcggc cgccaagaag tacgactgcg aggcttggat
     1321 accgagccag gagcggtacc gcgaactcac ttcgtgttcg aacaccaccg acttccaggc
     1381 ccggcgcatc ggatgccgct accgtcccga gcccggcgct cctacggagt tcgtcaacac
     1441 cctgaacggc accgcgatcg ccgtcggccg gacgatcatc gcattgctgg agaatcacca
     1501 gcaggccgac ggctcggtcc gggttccgga aatcctggtc gcctacgggg ccccggcgac
     1561 catcggcccc acgtgagcga ccaccgggtc ctgatctgcg acgaccactg gatcgtcagg
     1621 caggcgatcc ggcagcgcat cagtgagctt cccggcttcg aagtggtcga cgaggttgcg
     1681 cagggcaagg aagtgaagcc ggcggtgcgg gaacaccggc cggatctggt catcctggac
     1741 atcgaaatgc ccgacgtcaa cgggatccaa tccatggaac ggatcctgtc ccggttcccg
     1801 gagaccaggg ttctcgtctt caccgctcac gacgacccca atctggtccg ccttgcagcc
     1861 cgggaaggcg ccgatggctt cctggtgaag tcggccgctc tcgaccagat acgcgatgcc
     1921 tgcgagaccg tgatgtcggg aaagaagtgg ttcccgggat acagccgcaa acagctcgaa
     1981 gaggacgacg aactgaagcg gttcctttcc atttccgagc gggagcggga gatccttgcc
     2041 ctgctggcga ccggcatgag ggcccagggg gtggccgagg aaatcgggat ccggccggcc
     2101 acggtctaca cgcacgttcg caacgtggtc ggcaagctcg gagtcgagac ccggacccag
     2161 gcggtcgcga tcgcgacccg atattccttt ctggatccgg accccgaact ccggctcact
     2221 tcccgctgag gctcacttct ccttgaattc gagggcgcag ccgttgatgc agtaacgctg
     2281 cccggtgggg ccgggcccgt ccgggaacac atgtcccagg tgggcgtcgc agttcaggca
     2341 gacgacctcg gtgcggacca ttccgtggct tatgtcctgc ctcaactcga tgtgttcgag
     2401 attcgccggc tcggtgaaac tcggccagcc ggtgcctgaa tcaaattttg cgtccgagga
     2461 gaacagctcc ttgccgcaac atacgcagcc gtaggacccg ctacccttgt ggtcgaggta
     2521 gggacccgtg aaaggacgct cagtcgcggc ctttcgagtt acctcgtaag cctcgggaga
     2581 caactcctcc cgccattctg aatccgtttt cttcacgcgt gccgaattcg acattgccca
     2641 gtctcgcttt ctttttgcct gctttaaggg tagccaagcg gtcaaccccc ctcgcctgtc
     2701 ttgccctgct tctggccgcg gtgctgccgg tgatttcggc cggcccgctc ggtccgggcg
     2761 acgccgcggc tgccgggctg atcgctccgg ccaagtactg ctccgacaat gccgcaaacg
     2821 gcggccgggc cgagatggcc aaggcccgca ggtcgatgat ctgcatggtc aactacgccc
     2881 gacgcaggaa aggcatgagg ccctaccatc gtcagccgaa gctgaactgg tcgagcggac
     2941 gcaaggccag ggacatcctc cgctgcgggt tttcacacgg tgcctgcggt cgcgctttcg
     3001 actactggat ccgtcgctcc ggctacctcg gtggaggcgg ctgggcaacc ggtgagaaca
     3061 tcgcctgggg cagcggctcg ctcggcagcg tccgctcgat attcgtcgcc tggatgaagt
     3121 caaagggcca ccgggaagcg atcctcgaca gatccttcac cgatgtcggg gtcggcgtga
     3181 ccaggggccg attctccggc atagcgggcg cccggatctg ggtgctgcac ttcggctaca
     3241 actgattccg gccggatcgc gtccgaccgg ttcacaccgc aggttgcagc caggtagcga
     3301 accggcgggc aacctcccgg cccgttggtc gcgagggcta gcatgggggc caccgaggag
     3361 aaccggaaac gaccggagga gaagccagat gtcagaccag tcagagctcg atgtcccgct
     3421 caagacggag cagacctttc cgccccccga ggagtttgct gccgcgagcg aattcagcga
     3481 tcccgaaatc tacgcccggg ccgacgcaga tccggaaggc tggtgggccg gctgggcgta
     3541 gatttcggga tcgctgaatt c
//
LOCUS       DAOTIP010000119         3557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31115_length_3557_cov_3.188464, whole genome shotgun sequence.
ACCESSION   DAOTIP010000119 DAOTIP010000000
VERSION     DAOTIP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31115_length_3557_cov_3.188464"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="P5138_03250"
     CDS             <1..528
                     /locus_tag="P5138_03250"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRV59621.1"
                     /translation="HFDGRLEDLGAARAVCGLPILRKDFVVDEYQLYEAAVGGADAVL
                     LIAAVLDDDRLRDFQLKATELDLDCLVEIHDEEELDRAVESGAEVIGINNRNLDTGVV
                     DIQTTYELITDVPTGTTVVSESGISNREELIELERVGVDAALIGESLMRSDNPEEMVR
                     TLAGMSDNTTEHFLP"
     gene            878..2146
                     /locus_tag="P5138_03255"
     CDS             878..2146
                     /locus_tag="P5138_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRV59622.1"
                     /translation="MNETSAPPKRSVRSVFLAALMGGLVVAVAGYIAIAAGWIGTTET
                     TVQSMTATTTNASSNQDDQNLVNQIYNRDSDGVGFITASGITTESSSAFDPFGQQQGT
                     STATGSGFLIDTDGHMVTNNHVIEGAKKITVTLGDSDAVYDATVVGTDPSTDLALLKV
                     DAPQDEMKPLKLGDSANVKVGDPVVAIGNPFGLDRTVTTGIVSALQREIASLNQYAIS
                     NVIQTDAAINPGNSGGPLIDADGNVIGVNSQIATGSGSSTSGNVGIGFAIPSNTVKDV
                     VNQLLDDGTVEHAYLGISGASIDSQLAEALNLGTDQGVLVQEAPKDGPAAKAGIKAGD
                     TAVTVQGQRVMTGGDVITKVNGKELTSMEDLIAAVNDAKVGDKMELEVERDGDTSTVT
                     VTLGTRPDDSSSGSSSSSQGQQQLPPGFGQ"
     gene            2224..2862
                     /locus_tag="P5138_03260"
     CDS             2224..2862
                     /locus_tag="P5138_03260"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRV59623.1"
                     /translation="MKVKVCGITNRDDAEAAVEHGAWAIGLIHHKESPRFVKASVAAG
                     IGEEFRRRCEVVGVFVNPTIERVVQAAENAQLTMIQLSGNEGANFCSEVNRRTGLKVI
                     KAFHVASQADVKASAAYRFTDFHMFDTGRRGSYGGTGETFDWELLANYHSTIPFILAG
                     GLNPDNVTEAIRVVRPDAVDVASGVEKEPGIKDHGKLASFFQAARYTPVAGA"
     gene            2859..>3557
                     /locus_tag="P5138_03265"
     CDS             2859..>3557
                     /locus_tag="P5138_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV59624.1"
                     /translation="MSDSIAESTAGELPGRFGPYGGRYVPETLIPALDELTAAWAEAR
                     QDPAFTEELDVLLRDYVGRPSPLYLASRLSEEAGRKVYLKREDLNHTGAHKINNAIGQ
                     ALLAKRMGKPRIIAETGAGQHGVASATACALLDLECIVYMGSEDIRRQKPNVERMKLL
                     GAEVVPVEAGARTLKEAVSAAIRDWVTNVANTHYIIGSAVGPAPFPELVRDLQRTIGD
                     EARDQALQAEGSLPG"
BASE COUNT          707 a         1187 c         1127 g          536 t
ORIGIN      
        1 catttcgacg gtcgcctcga ggacctcggg gcggcgcggg ccgtatgtgg gttgccgatc
       61 ctccgcaagg acttcgtggt cgacgagtat cagctctatg aagcagcggt gggtggcgcc
      121 gatgcggtcc tcctgatcgc cgccgtgctc gacgatgacc gcctgcgcga tttccagctg
      181 aaggcgaccg agctcgacct cgattgcctg gtcgaaatcc atgacgagga agaactcgac
      241 cgtgcggtcg agtcgggagc cgaagtcatc gggataaaca accgcaacct cgacaccggc
      301 gtggtcgaca tccagaccac ctatgaactg atcaccgacg tcccgaccgg aaccaccgtg
      361 gtctcggaat ccggcatctc caaccgcgag gaactgatcg aactcgaaag ggtcggagtg
      421 gacgcagccc tgatcggcga atccctcatg cgctcggaca accccgagga aatggtcagg
      481 accctcgccg gaatgtccga caacaccacc gaacacttcc tgccctaggc ccggacggca
      541 ccggaccggc cgaacctccg ggtccacgtc tgaaccgggt cggatggccg tggggtcggg
      601 tcgatgtctc cggggaattg cgcagggttc ggtggtgtgc ttccgaaacc tctggatggg
      661 gagcgcgaag cgcgacggtt tcggaggtac accaccgggc gctgcaggaa cgccgaaggc
      721 aggccgcctt ttcgactggc tgcccggccc cgcggcgtaa cgaccggctc cacggccccg
      781 ccaccgggca acgggattct cagccgattc tcaggtttag aaaaccttag atttcggctt
      841 aggaatggcc aatgacggtg gtctttattt ccccgtcatg aacgagactt ctgctccccc
      901 caagcgctcg gtgcgctccg tgttcctggc cgccctgatg ggtggcctcg tcgttgcagt
      961 ggccggctac atagcgatcg ccgccggctg gatcggcacg accgagacga cggtccagtc
     1021 gatgaccgcg accaccacga atgcgtcatc gaaccaggac gaccagaacc tcgtcaacca
     1081 gatctacaac cgtgacagcg acggcgtcgg tttcatcacc gcctccggca tcaccaccga
     1141 gtcgagcagc gcattcgacc ccttcggcca gcagcagggc accagcaccg cgaccggctc
     1201 cggtttcctg atcgacaccg acggtcacat ggtcaccaac aaccacgtga tcgaaggagc
     1261 caagaagatc acggtcaccc tcggtgactc ggacgcggtc tacgacgcga ccgtcgtcgg
     1321 caccgacccc tcgaccgacc tggccctgct caaggtcgat gccccgcagg acgagatgaa
     1381 gccgctcaag ctcggcgatt ccgccaacgt caaggtcggg gacccggtgg tcgcgatcgg
     1441 caacccgttc gggctcgacc ggaccgtcac caccggaatc gtctccgccc tgcagcgcga
     1501 gatcgccagc ctcaaccagt acgcgatctc gaatgtgatc cagaccgacg ctgcgatcaa
     1561 ccccggcaac tccggcggcc cgctgatcga cgccgacggc aacgtgatcg gagtcaactc
     1621 gcagattgcg accgggtccg gatcgagcac cagcggcaac gtcggcatcg gcttcgccat
     1681 cccgagcaac accgtcaagg acgtggtcaa ccagctgctg gacgacggta cggtcgaaca
     1741 cgcctacctc ggaatcagcg gcgcgtcgat cgactcgcag ctggcggaag cactcaacct
     1801 cggcaccgac cagggcgtcc tggtccagga agccccgaag gacggaccgg cagccaaggc
     1861 cggaatcaag gccggtgaca ccgcggtcac ggttcagggc cagcgggtaa tgacgggcgg
     1921 cgacgtgatc accaaggtca acggcaagga actcacctcg atggaagacc tgatcgcggc
     1981 cgtcaacgac gccaaggtcg gcgacaagat ggaactcgag gtcgaacgtg acggcgacac
     2041 cagcaccgtg accgtcaccc tcggcacccg ccccgatgac agcagctccg gctcgagcag
     2101 ctccagccag ggtcagcagc aactgccgcc gggcttcggt cagtaacccc ccaaagcagt
     2161 ccctctcctg tcggcgggcc gtctggatgg cggcccgtcg tcgtgttgtc atacccttga
     2221 ctggtgaagg taaaggtctg cggaatcact aatagagacg atgccgaagc cgccgtggag
     2281 cacggcgcct gggcgatcgg gctgatccat cacaaggaaa gtccccgatt cgtcaaggct
     2341 tcggtcgcgg ccgggatcgg cgaggagttc cgtcgccgct gcgaggtggt gggagttttc
     2401 gtgaatccga ccatcgaaag ggtggttcag gcagccgaga acgcacagct cacgatgatc
     2461 cagctgagcg gaaacgaagg cgccaacttc tgcagcgagg tgaaccgccg gaccggcctg
     2521 aaggtgatca aggccttcca cgtggcgagc caggcagacg tgaaagccag cgccgcctac
     2581 cggttcaccg acttccacat gttcgacacc ggccgccgcg gctcttacgg cggaaccggc
     2641 gagaccttcg actgggaact gctcgcgaat taccactcga cgatcccgtt catcctcgcc
     2701 ggaggactca acccggacaa cgtcaccgag gcaatccggg tggtgcggcc cgacgcagtc
     2761 gacgtagcct ccggagtcga gaaggagccc ggaatcaagg atcacggcaa gctggccagt
     2821 ttcttccagg cggcccgcta caccccggtg gccggcgcat gagcgacagc atcgccgaat
     2881 cgaccgccgg cgagctgccg ggccgtttcg gtccatacgg cgggcgttac gtgcccgaga
     2941 cgcttatccc cgcgctggac gaactcactg ccgcctgggc cgaggcgcgc caggacccgg
     3001 ccttcaccga ggaactcgac gttcttcttc gcgactatgt gggccgaccc tcgccgcttt
     3061 accttgcgag ccgtctgagc gaggaagccg gacggaaggt gtacctgaag cgggaggacc
     3121 tgaaccacac cggcgcccac aagatcaaca atgcgatcgg ccaggcgctg ctggcgaagc
     3181 ggatgggcaa gccgcggatc atcgccgaga ccggggccgg ccagcacggg gttgccagcg
     3241 ccaccgcctg tgccctgctc gatctcgaat gcatcgtcta tatgggcagc gaggacatcc
     3301 ggcgccagaa gccgaacgtc gagcggatga agcttctggg agccgaagtg gtgccggtcg
     3361 aggcgggcgc gcggaccctc aaggaagcgg tcagcgcggc gatccgcgac tgggtgacca
     3421 atgtcgcgaa cacgcactac atcatcggtt ccgcggtcgg gccggctccg ttcccggaac
     3481 tggtccggga ccttcagagg acgatcggcg acgaggcccg cgaccaggcc cttcaggcgg
     3541 aaggctcatt gcccgga
//
LOCUS       DAOTIP010000120         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31137_length_3555_cov_3.159714, whole genome shotgun sequence.
ACCESSION   DAOTIP010000120 DAOTIP010000000
VERSION     DAOTIP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31137_length_3555_cov_3.159714"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="P5138_03270"
     CDS             <1..216
                     /locus_tag="P5138_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59625.1"
                     /translation="ASNAESLGGELGLLGGQTGGCPAVASLDQQLECGFEHGCAGRRS
                     PVLLLPAFPGHPTLSSGMPGIYNKSVN"
     gene            282..758
                     /locus_tag="P5138_03275"
     CDS             282..758
                     /locus_tag="P5138_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59626.1"
                     /translation="MNKDEKTNYAGAIYGTIVSMAVIATSAKDPSLGPVLIAFWAAIT
                     ALIFWLAHVYSDIVAEGYARFTEAGKVARVALRREWPLVQGALIPSAAMLLTVVTPMD
                     TDSATWVAMAAGLVVLFCTGLLIGARDGMTWGRRILIGSINACFGLVIVALKVWVH"
     gene            complement(772..1044)
                     /locus_tag="P5138_03280"
     CDS             complement(772..1044)
                     /locus_tag="P5138_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59627.1"
                     /translation="MGCFVVLFALISPRLALFFILIFSDWIGQAIDGWVVPMLGFFFL
                     PWTLLAYVVMWQVSPHGVEGFDWFIVGFGFVVDIASYARTSQSRQN"
     gene            complement(1054..1878)
                     /locus_tag="P5138_03285"
     CDS             complement(1054..1878)
                     /locus_tag="P5138_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRV59628.1"
                     /translation="MSFDRSAGTRQAAQLSFYVLMTFPALLLLAVWILSNVFDSPGVR
                     EDLIREIIGNLPLQEVEGRKELENLLDQLTRGAGGIGLVTAAVLFYSSSAAIGALRHA
                     VETANEKGLSGPPFPKNKGLDILIMLVTLPAALIFISLVLSRDLATVVDESNLLTWVA
                     ANLGGPAGIFAAGVIFYTWIFWVLNPGHSTWSSAAVGAVFASLVIWLVWAGLKVWFGI
                     SGGGSTVYGVLAGFLGMLLFLNLASMGVVLGAHVSAIWRVHRESGGSDPDAAAVKG"
     gene            complement(1970..2398)
                     /locus_tag="P5138_03290"
     CDS             complement(1970..2398)
                     /locus_tag="P5138_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59629.1"
                     /translation="MPETSGSQPPGGSAAWASWVAFAGIMLMLIGVFSVIAGLAALTD
                     DGYIKNASLHEVFLVSFKALGVFWILIGILKVMTGWALIQGREWARIVTVILVFLSAI
                     SHLLDLTSQPYLSVLMIALDVVILYGVTVRWQAAKIGMGD"
     gene            complement(2464..3345)
                     /locus_tag="P5138_03295"
     CDS             complement(2464..3345)
                     /locus_tag="P5138_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRV59630.1"
                     /translation="MGSERHYPEERPVGPMTVDGFWMDETTVTNRQFAEFVESTGHVT
                     TAEKAPDPAMYPGADPSLLVPGSITFAKPNGPVDLSNPMQWWAWTPGAQWRHPWGPSS
                     NLEGKEDHPVTQVTWEDAEAYARWAGKQLPTEVQWERAGRGGIEAAEFAWGDELAPGG
                     EERMNRWIGEFPWKFRPRPGGARKPGTVTVKTFAPNPFGLYEITGNVWEWTSDWYSDS
                     HPEPASRCCAPVNLRGVSVEASRDPASGIPRKVLKGGSFLCAENYCSRYRPAARIPES
                     IESSTVHISFRCVRPAG"
BASE COUNT          666 a         1164 c         1109 g          616 t
ORIGIN      
        1 gctagcaatg ccgaatcact cggcggcgag cttggccttc tcggcggcca gacgggtgga
       61 tgtcccgccg tagcctctct cgaccagcaa ctcgaatgtg gcttcgagca cggctgcgcg
      121 ggtcgccgca gtccggtcct tctgctgcct gcgttccccg gtcacccgac cctctcctcc
      181 ggcatgcccg gaatatacaa caagtcagtc aactgacttg taagtttgaa cggcttcggc
      241 atggccattt tccgggaggg gctgctctat cgttcccggc cttgaacaag gacgagaaga
      301 cgaactacgc cggtgcgatc tacggaacca tcgtctcgat ggccgtgatc gccacttcgg
      361 ccaaggaccc ttcgctgggt cctgtgctga tcgcgttctg ggccgccatc acggcgctca
      421 tcttctggct ggcccacgtc tactccgaca tcgtggccga gggctacgcc cggttcacgg
      481 aagccggcaa ggtcgcaagg gtcgccctgc gccgggaatg gccgctggtc cagggggcac
      541 tgatcccctc ggcggccatg ctgctaaccg tggtcacgcc gatggacacg gactcggcga
      601 cctgggtggc gatggcagca ggccttgtcg tgctcttctg caccgggctg ctgatcggag
      661 cccgggacgg aatgacctgg ggccggcgca tcttgatcgg cagtatcaat gcctgcttcg
      721 gactggtgat tgtcgccctg aaagtctggg tccactaggg accatggaaa ttcagttctg
      781 cctggactgg gaggtgcgtg cgtacgaggc gatgtccacc acgaagccga agccgacgat
      841 gaaccagtcg aaaccttcca ccccatgcgg gctcacctgc cacatcacca cataggcgag
      901 cagggtccag ggcagaaaga agaagcccag catcgggact acccagccgt cgatggcctg
      961 accgatccag tcggagaaga tcaggatgaa gaacagcgcc aggcgcggtg agatcagtgc
     1021 gaagagaaca acgaagcagc ccatggcctg aatctatccc ttcacggcgg cggcgtcagg
     1081 gtcgctcccc ccggattcac ggtgaacgcg ccatatcgcg gacacgtgag cacccagaac
     1141 gacccccatc gaagcgagat tcaggaagag cagcatgccc aggaagccgg ccaggacccc
     1201 gtacacagtg ctgccgcctc cggagattcc gaaccagacc ttcaggccgg cccagaccag
     1261 ccagatgacc agagaggcga agaccgcgcc aaccgcggcg ctggaccagg tggaatggcc
     1321 cgggttcagg acccagaaga tccatgtgta gaagatcact cccgccgcga agattccggc
     1381 cggaccgccg agattcgcgg caacccaggt cagcaggttg ctctcgtcaa ccaccgtggc
     1441 gaggtcccgg gagaggacca gggaaatgaa gatcagcgca gccggcagcg tgaccagcat
     1501 gatcaggatg tccagtccct tgttcttcgg gaacgggggt ccggacagcc ccttttcatt
     1561 ggcggtctcc accgcatgcc gcagggctcc gatcgcggcg ctgctcgagt agaagaggac
     1621 tgctgccgtc accaggccga tcccgcccgc acctcgggtc aactgatcga ggagattctc
     1681 gagttccttg cgcccctcga cctcctgcag gggcaggttc ccgattattt cccggatcag
     1741 gtcttcgcga acgcccggcg aatcaaagac gttgctgagg atccagacgg ccagcagcag
     1801 cagtgccgga aaggtcatca gcacgtagaa gctgagttgt gccgcctgcc gggtccccgc
     1861 cgaacgatcg aacgacagga gaacttccca gcaggcggcc ccgaaccgac gcagcggttc
     1921 gggcagcttc gatacccagg ccttcagctt tgaagcccgg gcctgcgagt cagtccccca
     1981 tgccgatctt cgcggcctgc cagcgaaccg tcacgccgta gagaatgacc acgtcaagag
     2041 cgatcatcag gacgctcagg tagggctgcg aggtcaggtc gagcagatga ctgatcgccg
     2101 agaggaagac aaggatcacg gtaacgatcc gggcccattc tcgaccctgg atcagcgccc
     2161 atccggtcat caccttgagg atcccgatca ggatccagaa cacacccagg gccttgaaac
     2221 tgacaaggaa gacctcgtga agggatgcgt tcttgatgta tccgtcgtcg gtcagcgcgg
     2281 ccaggccggc aatcacgctg aagacgccga tcagcatcag catgatgccg gcgaacgcga
     2341 cccatgacgc ccaggcggcc gaacctcccg ggggctgtga accactggtt tcaggcattg
     2401 aactcctcca tcggtttcat gtttggcagt ccgccggggg acggcccggc ggccgcatga
     2461 accctatccg gccggtctga cgcagcggaa ggagatgtga accgtggagc tctcgatgct
     2521 ttccgggatc cgcgcggcag gccggtagcg ggagcagtag ttctcggcac agaggaaaga
     2581 cccgcccttg aggaccttcc gtggaatgcc gctggctgga tcgcggctgg cttcgacgct
     2641 cactccccgg agattgaccg gagcacagca gcgactggcc ggttccgggt ggctgtccga
     2701 ataccagtcg ctcgtccact cccagacgtt gccggtgatc tcgtagaggc cgaagggatt
     2761 cggagcaaag gtcttgaccg tcaccgtgcc gggtttcctg gcaccgccgg gccggggccg
     2821 gaacttccag gggaactccc cgatccagcg gttcatccgt tcctcgcccc cgggagccag
     2881 ttcgtctccc caggcgaatt cggccgcctc gattccgccc cgcccggccc gctcccactg
     2941 gacctcggtc ggaagctgct tgcctgccca gcgggcgtag gcctccgcgt cctcccaggt
     3001 cacctgggtc accggatggt cttcctttcc ttccaggttg ctggagggcc cccaggggtg
     3061 ccgccactgc gcgccggggg tccaggccca ccactgcatc ggattggaaa ggtcaaccgg
     3121 gccattcggc ttggcgaagg tgatcgagcc gggaaccagc aacgaagggt cggcaccggg
     3181 gtacatggcc ggatccgggg ctttctctgc agtcgtgaca tggcccgtgc tttcgacgaa
     3241 ttccgcgaac tggcggttgg tcaccgtggt ctcgtccatc cagaaaccat cgaccgtcat
     3301 cggccccacg ggccgctctt cggggtagtg cctttcgctg cccatctgtt aggtccctcc
     3361 cgggatccag accattccct gggcgggcgg cggtcccggc ggttcctttc acctcaggca
     3421 ggcgggcata gaagatctgc cgcgagccga ttatcgtgac ctcttcggcc atctccttcg
     3481 tcggggccac cccttccacc acgatgcaga gctcgtggga gcggatttcc ttgatcggct
     3541 cgaggtcacc catga
//
LOCUS       DAOTIP010000121         3545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31251_length_3545_cov_3.417192, whole genome shotgun sequence.
ACCESSION   DAOTIP010000121 DAOTIP010000000
VERSION     DAOTIP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31251_length_3545_cov_3.417192"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="P5138_03300"
     CDS             complement(<1..1705)
                     /locus_tag="P5138_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59631.1"
                     /translation="MTSPKTMKVLIGFSCSLALGLACLWLLAGPASAGLVRTGGWESE
                     NTVRPFNLSSVTVDDDGNAYVADYGLTRIVRIDASGDADLNWGTGTPGLSYPPNPVDL
                     TTDDDGNVYALDMDGKKVRKYSSSGNLLTSWPVSSAYYGGRWSIAWESGKVWVLTENK
                     VSEFEPDGTPVALGQDAFDFDSIPNYVTPQIAGDGNGHLVIASAAWVTEGTSATRSIY
                     RFDTDGSLLSKWTSPATVTHVSLPAGGSYYTVDAYYSLAVDPSGDIWVSTAGSDHPFR
                     VVDASGLQPGGVGDLTWGGDLTIDNDNGFWTIRYNPDHLAESQVRRSEIDGSGLQIWG
                     AGDFPDYADQVWPASFKWINDYSVDSSGNVAILDLGFHRVKIFNADGDFLRGFKVPEG
                     VGSDPDYVARVNLKDDGTVEVFDGKDGKLRRYDSSGALTGESVLDVPGRVKQFAPTAD
                     GGHLIVSRSGSVTNFDANDLETGSWSLQPHGSDIDNVKGIVQAPAGDVYVLTPDFLDH
                     YSAGGELLGTWSLRDEECAEEWSDGFVDLDVDAAGNVYATASLGAWINWTKLYAFDPE
                     LN"
     gene            1835..2680
                     /locus_tag="P5138_03305"
     CDS             1835..2680
                     /locus_tag="P5138_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRV59632.1"
                     /translation="MIEGGGMRGAVSGGMAMALHELGLAESFDAAYGSSAGALNALWL
                     ISGRAEDGMPTWTDAELGKDLMSKRRIFGKRPIVDMKTMIEQNYEIVSPGLFEAVLAA
                     DTEFHPLATDIETGLAVDLHDQVRDRESLILAARATSNLPLIGGPPVAMNGGRYLDAG
                     LSAAIPFQAAIDDGATHLLVLRSRKEGDVASDPSGIGGRLTSRFLGRISPAVREGYLT
                     RGAREGADEHFLARHDSEIGVNPSILQIRPHPDSPVPSRLESDMDVVEAGLESGRQAV
                     LEALG"
     gene            2685..3494
                     /locus_tag="P5138_03310"
     CDS             2685..3494
                     /locus_tag="P5138_03310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRV59633.1"
                     /translation="MVKVITALLAGLLIGAGVVALFGDGSDDDSDKASATNPLGVSKV
                     IDLSHTVDENIPLWPGDPKMKFTTVANFKDDGYYLRKFQIGEHSATHMNAPNSFHADG
                     VGIDYYKPEDLIREAVVIDVKDQAAADPDYAFSIDDLKAWEKQNGEVPADSVVLLNTG
                     WTKYWRNPQKFFGLDGKGGLHFPGFAGSTTKYLLDQRDIAGVGIDTHGVDPGQDETYA
                     TNTQVLSQPRIVLENLTNLDSMPATGASIVIAPLKLKDGSGSPATVTGLIP"
BASE COUNT          677 a         1140 c         1131 g          597 t
ORIGIN      
        1 ggttcagttc cggatcgaag gcgtagagct tggtccagtt gatccacgct ccgagcgagg
       61 ctgtcgcata cacattgccg gcggcgtcaa catcgagatc gacaaagcca tctgaccatt
      121 cctcggcgca ttcctcatcc cggagcgacc aggtgccgag caactcgccg cctgccgagt
      181 agtgatcgag gaaatccggc gtcagaacgt agacgtcgcc ggcaggagcc tgaacgatgc
      241 ccttcacatt gtcgatatcg gacccgtgcg gctgcaggct ccagcttccg gtttccaggt
      301 cgttcgcatc gaagttggtg accgagccgg acctcgagac gatcaggtgc ccgccatccg
      361 cggtgggggc gaactgtttc accctgccgg gaacgtcgag tacggactcg ccggtcaggg
      421 caccggagct gtcgtagcga cgaagcttcc cgtcttttcc atcgaatacc tcgaccgtcc
      481 cgtcatcttt gaggttcacg cgagcgacgt agtccgggtc gctgccgacc ccttcgggaa
      541 ccttgaaccc cctgaggaaa tctccgtcag cattgaagat cttgacccgg tgaaacccga
      601 gatcaaggat cgcgacgttg ccggacgaat cgaccgaata gtcgttgatc cacttgaagg
      661 acgcgggcca tacctgatcg gcgtaatccg gaaaatcgcc ggcaccccag atctggaggc
      721 ccgacccgtc gatttcgctc cgccggacct ggctctcggc gagatggtcc gggttgtacc
      781 tgatcgtcca gaacccgttg tcattgtcga tggtcaggtc tccgccccag gtcaggtcac
      841 cgaccccgcc cggctgcagt cccgaagcat cgaccaccct gaagggatgg tcggagccgg
      901 cggtcgaaac ccagatatcc cccgaggggt cgaccgcgag actgtagtaa gcgtcaacgg
      961 tgtagtagct gcctcctgcg ggaaggctca catgagtcac ggtggccggg gacgtccact
     1021 tcgaaaggag cgaaccgtcc gtgtcgaacc gatagatcga cctggtcgcc gatgttccct
     1081 ccgtcaccca tgccgccgag gcgatgacca ggtggccgtt cccgtcgccg gcgatctgcg
     1141 gcgtgacgta attcggaatc gaatcgaagt cgaaagcgtc ctgaccaagc gcaactggtg
     1201 tgccatccgg ttcgaattcc gaaaccttgt tctcggtcag gacccacacc tttcctgact
     1261 cccacgcaat ggaccatcta ccgccgtagt aggcggaact gaccggccaa ctggtcagca
     1321 agttgcccga tgacgagtac ttgcggacct tcttgccatc catgtcgagg gcatagacgt
     1381 tgccgtcgtc atcggtggtg aggtcgaccg gattaggcgg gtagctcagg cccggggtac
     1441 ccgtgcccca gttgaggtcg gcatcgccgc tggcatcgat cctgacgatc cgggtcagtc
     1501 cgtaatccgc gacgtaggcg ttgccgtcat cgtcgaccgt gaccgaagaa aggttgaagg
     1561 gacgaaccgt gttctcgctc tcccagccgc cggtcctgac caggccggca gaggccggac
     1621 cggcaagaag ccagagacag gcaaggccca gggccaggct gcatgagaag ccgatgagca
     1681 ccttcatggt cttcggtgaa gtcatgttcc cccgggtcga ctggtgtcag ggtatccggc
     1741 gatgcgataa aacgggtccg tggcaggcac cgctgaaatc catcccgtac tgcgtgcaat
     1801 aagggaaccc gcgcaggggg acagggttgc gctggtgatc gaagggggcg gcatgcgcgg
     1861 tgccgtttcc ggagggatgg cgatggccct gcatgagctc ggcctggccg aaagcttcga
     1921 tgctgcctac ggctcctcgg ccggggcgct gaacgccctc tggctgatca gcggcagagc
     1981 cgaggacggc atgccgacct ggaccgacgc agaactcggc aaggacctga tgagcaagcg
     2041 gcggatcttc gggaagcgtc cgatcgtcga catgaagacg atgatcgagc agaactacga
     2101 gatcgtctcc cccggcctct tcgaggccgt ccttgctgcc gacaccgaat tccatccgct
     2161 ggccaccgac atcgagaccg gcctcgcggt cgatctccac gaccaggtca gggaccggga
     2221 gtcgctgatc ctcgccgccc gggcaacttc gaacctgccg ctgatcggcg gcccgcccgt
     2281 cgccatgaac ggcggccggt acctggacgc cgggctcagc gcggcgatcc ccttccaggc
     2341 cgcgatcgac gacggcgcca ctcacctgct ggtcctgcgg tcacgcaagg agggtgacgt
     2401 cgcaagcgat ccctcgggaa ttggcggacg gctgaccagc cggttcctcg gccggatcag
     2461 tccggcggtg cgcgagggat acctgacccg gggcgcacgt gaaggggccg atgagcactt
     2521 cctggcccgc cacgacagcg agatcggggt caatccgagc atcctgcaga tccgtcctca
     2581 tcccgactcg ccggtgccct cccggctcga atccgacatg gacgtggtcg aggccggcct
     2641 ggaaagtggt cgccaggcgg tgctggaggc gctaggttga gcctgtggtc aaagtcatca
     2701 cagccctcct cgcgggtctc ctgatcggtg ccggagtggt cgccctgttc ggtgacggat
     2761 ccgatgacga cagcgacaag gcctctgcga ccaacccgct gggggtctcg aaggtcatcg
     2821 atctgagcca caccgtcgac gagaacatcc cgctctggcc gggcgatccg aagatgaagt
     2881 tcacgacggt ggccaacttc aaggacgacg gctactacct gcggaagttc cagatcggcg
     2941 agcacagcgc cacccacatg aacgcgccca attccttcca cgctgacggg gtcgggatcg
     3001 actactacaa gcccgaggat ctgatccgcg aggcggtcgt gatcgacgtc aaggatcagg
     3061 ccgccgccga tccggactac gcgttcagca tcgacgacct caaggcgtgg gagaagcaga
     3121 acggcgaggt gccggccgac agcgtggtcc tgctcaacac aggctggacg aagtactggc
     3181 gaaacccgca gaagttcttc ggtctcgacg gcaagggcgg cctccacttc cccggctttg
     3241 cgggatcgac gacgaaatac ctgctggacc agcgcgacat cgccggcgtc gggatcgaca
     3301 cccacggggt cgacccgggc caggacgaga cctacgcgac caacacccag gttctgagcc
     3361 agccccggat cgtgctggag aacctgacca acctcgacag catgccggcg accggggcga
     3421 gcatcgtgat cgcacccctc aaactgaagg acgggtccgg ctcgccggca acggtcacgg
     3481 ggctgattcc ctgaccatga agtgggcggt ggccggggcg ctcgcgctgg catgcctggc
     3541 cggga
//
LOCUS       DAOTIP010000122         3538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31332_length_3538_cov_3.213322, whole genome shotgun sequence.
ACCESSION   DAOTIP010000122 DAOTIP010000000
VERSION     DAOTIP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31332_length_3538_cov_3.213322"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="P5138_03315"
     CDS             complement(<1..572)
                     /locus_tag="P5138_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV59634.1"
                     /translation="MNEAVRADEARYVMQTYKRAGVEFVRGEGALLFDDEGREYVDFL
                     GGISVASVGHCHPKVVEAIRSQSAELLHVSNLFYTAPMVELAKRLSETSLGGKVFFCN
                     SGTEANECAIKIARKHARSRGVEKPEIVSFEGDFHGRSYGSLSATPGLAANPDFAPML
                     PGFRSVPFDDASALREAIGPDTAAVLIEPI"
     gene            complement(569..1468)
                     /gene="argB"
                     /locus_tag="P5138_03320"
     CDS             complement(569..1468)
                     /gene="argB"
                     /locus_tag="P5138_03320"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRV59635.1"
                     /translation="MDSQDKAKQIADTIGGETRLDDENVNVLLEALPYMREFHGKTVV
                     IKYGGAAMRDESLRQAFADDVVLLKYVGLNPVIIHGGGPEITKYMNRMGIEVRFQHGL
                     RVSDAETVEVAKMVLLGKLNAELVSRLNRAGSQAVGLSGEDGGLFEVAPVANAEEVGF
                     VGDIERVDIDVLNHISEDYIPVIASVGSDREGNSYNINADEAAGKVAAALGAFKVVFL
                     TDIVGWLNDPEDESSRISVTSVAEVEDALESISGGMRPKLTACVNAIRGGAQNAYIID
                     GRRPHSLLLELFTDAGIGTMVTP"
     gene            complement(1511..2698)
                     /gene="argJ"
                     /locus_tag="P5138_03325"
     CDS             complement(1511..2698)
                     /gene="argJ"
                     /locus_tag="P5138_03325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRV59636.1"
                     /translation="MSGHPSLSVPPEGEFFRSRWVEAPEGISFNPDGTLPEGLRAGGV
                     HCGLKGGGATDLGAVICESEEVASAILLTANAAAAAPIRVCRENLWQGEIRSVIVNSG
                     NANAATGEQGITDALSMRNSLATELGLAPQTVAVAETGVIGVPMDMDAVLSGISGLAS
                     SLTTDGAVAFSEAIMTTDKWPKRVSLTVDGITLSAQAKGAGMIEPGFATMLCFVQTDA
                     VVPDPEGALRQAVGGSFERITVDGQMSTNDTVLLQASGISGRELPEGLLDSVLLQLAL
                     EIVSDGEGASRVGRVEVTGAADSAEAEMVARKIANSPLVKTALLGRDPNWGRIAQAAG
                     QALAGQDIGELGPEVIEAGDIAAGGREPELALRLDRGNASAHVFTSDLTHEYIVINAE
                     YTT"
     gene            complement(2695..>3538)
                     /gene="argC"
                     /locus_tag="P5138_03330"
     CDS             complement(2695..>3538)
                     /gene="argC"
                     /locus_tag="P5138_03330"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRV59637.1"
                     /translation="EIQEPHLAEFDEVEVGIVAYPHGAAAPAVAEMRGRGLMVVDLSA
                     DFRFFSLPAYEKAYGPHSEPQLLEGAVYGLPELNRKRVVAGELIANPGCYPTATILGL
                     APLARRGLLDDVVIDAKSGVSGAGRNKGEATGFVQITENVAAYKPSGHRHRPEISEQL
                     GLLHPEGKAPETLTFVPHLVPLDQGELVTCYVRPTREVDQDELDGMYAEDYSDEPFVK
                     VRDVPPGMRDVRETNQCHVMALAGDDRIVVMSAIDNLWKGASSQAIQNLNLMLGLDET
                     EGLR"
BASE COUNT          575 a         1198 c         1128 g          637 t
ORIGIN      
        1 tggatcggct cgatcagcac ggcggcggtg tccggaccga tcgcttcccg cagcgcggag
       61 gcgtcatcga agggcaccga ccggaaaccg ggcagcatcg gcgcgaaatc cgggttcgcg
      121 gccaggccgg gagtggccga aagcgaaccg tagctgcggc cgtggaaatc gccctcgaaa
      181 gagacgatct ccggtttttc gacaccgcga gaccgggcat gtttgcgggc gatcttgatc
      241 gcacattcgt tggcctcggt gccggagttg cagaagaaga ccttgccgcc gagcgaggtc
      301 tcggaaagtc gtttggcgag ctcgaccatc ggcgcggtgt agaagaggtt cgaaacatgc
      361 agcagttccg cggactggga cctgatcgcc tcgaccactt tcgggtggca gtggccgacc
      421 gaggcgaccg agattccgcc caggaagtcg acgtactccc ggccctcgtc gtcgaagagc
      481 agcgccccct cgccccggac gaactcgact cccgcccgct tgtaggtctg catgacgtat
      541 ctggcctcgt cggcccggac cgcttcgttc atggcgtgac catcgtgccg ataccggcgt
      601 ctgtgaacag ttcgagcagc agcgagtgcg gtcggcggcc gtcgatgatg taggcgttct
      661 gggccccgcc ccggatcgcg ttgacgcagg cggtcagctt gggacgcatg cccccggaga
      721 tcgattcgag ggcgtcttcc acctcggcca cgctggtcac cgaaatcctc gaagactcgt
      781 cctccgggtc attcagccag ccgacgatgt cggtcaggaa gaccaccttg aaggcaccca
      841 gcgcggcagc gaccttgccc gctgcctcgt cggcattgat gttgtaggag ttgccttccc
      901 ggtcggagcc gaccgaggcg ataaccggga tgtagtcctc gctgatgtgg ttcaggacat
      961 cgatgtcgac ccgttcgatg tcgccgacga atccgacctc ctcggcattg gcgaccggcg
     1021 cgacctcgaa gagaccacca tcctcacccg acaggcccac ggcctgcgag ccggcccggt
     1081 tcaggcggct gaccagttcc gcgttcagct tgccgagcag gaccattttg gccacctcga
     1141 cggtctcggc atcggagacc cgcagtccgt gctggaagcg aacctcgatt cccatccggt
     1201 tcatgtactt ggtgatctcg gggccgccgc cgtggatgat caccggattg aggccgacgt
     1261 acttgagcag caccacgtcg tcggcgaagg cctgccggag cgactcgtca cgcattgccg
     1321 cgccgccata cttgatgact accgtcttgc cgtggaactc gcgcatgtag ggcagggcct
     1381 cgagcagcac gttgacgttt tcgtcgtcga gtcgggtctc gccaccaatg gtgtcggcga
     1441 tctgctttgc cttgtcctgg gaatccattt cgctttcctt cggtcggacc ggccggggcc
     1501 ggtatgggta ctacgtcgtg tactccgcgt tgatcacgat gtattcgtgg gtcaggtcgc
     1561 tggtgaagac atgcgccgag gcgttgccgc ggtccagcct cagcgccaac tccggctcgc
     1621 ggccccctgc ggcgatgtcg ccggcctcga tcacctcggg cccgagttcc ccgatgtcct
     1681 ggccggccag cgcctggcct gcagcctgcg caatccggcc ccagttcggg tccctgccaa
     1741 gcagcgccgt cttgaccagc ggcgaattgg cgatcttccg ggcgaccatc tccgcctcgg
     1801 cggagtcggc ggctccggtc acctcgaccc ttcccacgcg gcttgcacct tcgccatcgg
     1861 agacgatctc cagggcgagc tgaagcagca ccgaatcgag cagtccctcg gggagctccc
     1921 gccccgagat gccgctggcc tggagcagca cggtgtcgtt ggtgctcatc tgaccgtcga
     1981 ctgtgatcct ttcgaaggac ccgccaaccg cctgccgcag ggcaccttcc gggtcgggga
     2041 cgactgcgtc ggtctggacg aagcagagca tcgtcgcgaa accaggttcg atcatgccgg
     2101 ctcccttcgc ctgggcagag agcgtgattc cgtcaacggt gaggctgacc cgcttgggcc
     2161 acttgtcggt ggtcatgatc gcctcggaaa acgcgacggc tccatcggtg gtcaatgacg
     2221 aagcgagccc agagataccg gagagcaccg cgtccatgtc catcggcacc ccgatcacgc
     2281 cggtctcggc gaccgccacg gtctgcgggg ccaggccaag ctcggtagcg agcgaattgc
     2341 gcatcgaaag tgcgtcggtg atgccctgct ccccggtcgc cgcatttgcg ttgcccgagt
     2401 tcacgatcac cgaccggatt tccccctgcc agaggttctc ccggcagacc cggatcggtg
     2461 cggccgcggc cgcgttcgcg gtgagcagga tcgccgaagc gacctcttcg ctctcgcaga
     2521 tcaccgcgcc caggtcggtt gcccctcccc ccttgagacc gcagtggacc ccgccggccc
     2581 gtaaaccctc cggcagcgtc ccgtccggat tgaagctgat gccttccggc gcctctaccc
     2641 accgcgagcg gaagaactcg ccttcgggcg gcacgctcag gctcgggtgg ccgctcatct
     2701 cagcccctcg gtctcgtcga ggccgagcat caggttcagg ttctggatcg cctgagagga
     2761 agcgcccttc cagaggttgt cgatcgccga catcaccacg atccggtcgt ccccggccag
     2821 cgccatgacg tggcactggt ttgtttcccg cacgtcacgc atgcccggcg gcacgtcacg
     2881 gaccttgacg aagggctcgt ccgagtagtc ctctgcatac atcccatcga gctcgtcctg
     2941 gtcgacttcc cgggtcggcc ggacgtagca ggtgaccagt tcgccctgat caagtggcac
     3001 caggtgggga acgaaggtca gggtttcggg agccttccct tcgggatgga gcagaccgag
     3061 ctgctcgctg atctcgggcc ggtgccgatg gccggagggc ttgtaggcgg ccacgttctc
     3121 cgtaatctga acgaatccgg ttgcttcacc cttgttacgg ccggccccgg aaacgccgga
     3181 tttcgcatcg atcaccacgt catcgagcag ccccctccgg gcaagcgggg caagaccgag
     3241 gatggtcgcg gtcgggtaac agcccggatt ggcgatcagc tcaccggcca caacccgttt
     3301 ccggttgagc tccggcaggc cgtagacggc tccctcgagc agctgcggct cggagtgagg
     3361 tccgtaagcc ttctcgtagg cgggcaggct gaaaaagcga aaatccgccg agaggtccac
     3421 caccatcagg cccctccccc gcatctcggc gaccgccggg gcggcagccc cgtggggata
     3481 ggccacgatg ccaacctcga cctcgtcgaa ctcggccagg tggggttcct ggatctcc
//
LOCUS       DAOTIP010000123         3512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31619_length_3512_cov_3.505351, whole genome shotgun sequence.
ACCESSION   DAOTIP010000123 DAOTIP010000000
VERSION     DAOTIP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31619_length_3512_cov_3.505351"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="P5138_03335"
     CDS             complement(<1..355)
                     /locus_tag="P5138_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV59638.1"
                     /translation="MATTSDKSPKASAAGSRSLSTAEERREELISAAIPVFAEKGYHA
                     APTTEIARKAGISQAYLFRLYPTKEDLFVAAVERSTDNMVKTLAAAAEKAEQEGIEPG
                     DAMGLAYGELVSSDRE"
     gene            complement(442..672)
                     /locus_tag="P5138_03340"
     CDS             complement(442..672)
                     /locus_tag="P5138_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59639.1"
                     /translation="MGDLLRRRWRRILIVVIIVAVLGGLIFGPVAVKVFGTLAGLFAI
                     LAIIISGNDGGGGINPHQYQSGATGGGGAGGI"
     gene            complement(740..1030)
                     /locus_tag="P5138_03345"
     CDS             complement(740..1030)
                     /locus_tag="P5138_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59640.1"
                     /translation="MEIRARRFATPALLLVLGVSTWAMVMVPAVSGIATWLFILSGIA
                     VLYIFLFKAPGDPATGGQGSGGASDSGSGFGGFTGGSDCGGGHGGGDGGGGC"
     gene            complement(1249..>3512)
                     /locus_tag="P5138_03350"
     CDS             complement(1249..>3512)
                     /locus_tag="P5138_03350"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRV59641.1"
                     /translation="SGPVSFMRGADSWAGTIKSGGKTRRAAKMVVLDVDHPDVEHFIT
                     CKADEEKKAAALRDAGFDMSIDGDGFTSIQYQNANNSVRVSDEFMQAVADDAEWNLLA
                     RTDGSVTKTLSARELMNKIAEAAWDCADPGVQYDTIINKWHTCPESGRINASNPCSEY
                     MHVDDSACNLASINLMKFRRPDGSFDVDGFCAVVDTVFLAQEIIVGPSSYPTEEIGKN
                     ARAFRQLGLGYANLGALLMSDGMPYDSEEGRNVAAAITSLMTGRAYRRSAEIAATMGT
                     YEAYELNREPHNNVMRMHRDAAYEINGEGVHEELLSAAREEWDVAVATGEEHGYRNAQ
                     ATVLAPTGTISFLMDCDTTGIEPDFSLVKFKELVGGGNMTIVNKSVPLALQTLGYSES
                     EIGQIEAYINEHGTIIGAPGLKDEHLPVFDVAVGERAISHTGHIDMMAATQPFLSGAI
                     SKTVNMPNTATVEDIADAYTRGWEGGLKALAIYRDGSKTAQALRTDAQKDKEKEKEAA
                     AEAAPAGPVRHRMPKTRDSVTHKFSVGGHEGYITAGKYADGSVGEIFLTDIGKEGSTM
                     RGMMNAYATAISLGLQYGVPLEVFVNKFSYMRFDPEGITGNPDIPFAKSMPDYIMRWL
                     ASQFIDDPDFLEEQGIMTQEVRNRKEAENAGINLANGSGNGSPAGNGGNGNGGNGGPA
                     PEAEAPVADTGGTSVPAGTALTDDVPVVPAKLHFTPGAAELGPACDQCGGMMQRTGAC
                     YTCSSCGNNTGCG"
BASE COUNT          535 a         1152 c         1168 g          657 t
ORIGIN      
        1 gctcgcggtc gctgctgacc agctcgccgt aggcgaggcc catggcgtcg ccgggttcga
       61 tcccttcctg ctcggccttc tcggccgccg cggcgagggt cttgaccatg ttgtcggtgg
      121 agcgttcgac cgccgccacg aacagatctt ccttggtcgg atagaggcga aagaggtagg
      181 cctgggagat gccggccttc ctcgcgatct ccgtggtcgg agccgcgtgg tagcccttct
      241 cggcgaagac cggaatcgcc gccgaaatca gctcttcacg ccgctcctca gcggtggaaa
      301 gagatcggga tccggctgcg gatgccttgg gactcttgtc ggaagtcgtc gccatcaggt
      361 gtgagtatgc actcactcac accatttgtc aagtgcaact agcggccggg cacgatttgc
      421 ggcgtctttc cggcctgcaa ttcagatgcc gccggcaccg ccaccaccgg tggcaccgct
      481 ctggtactgg tggggattta tgcccccgcc accgtcgttc ccggagatga tgatcgccag
      541 gatcgcgaac agcccggcca gggtgccgaa taccttgacc gccacgggac cgaagatcag
      601 cccgccgagc acggcgacga tgatcacgac gatgagaatg cggcgccacc tcctgcggag
      661 cagatctccc atgcctggat cctagccgcg ctccgacctg tcgaccgggg aagctccgcc
      721 tgaggcgatc ggcgcggcat caacagccgc caccaccgtc tccgcctcca tggcctcctc
      781 cacagtcgga gccgccggtg aatccgccga acccgctgcc cgaatcgctc gcacccccgg
      841 acccttgtcc gccggttgcg ggatcgccgg gagccttgaa caagaagatg tagagcaccg
      901 cgatgccgga caggatgaag agccacgtgg cgatgccgct caccgccggg accatgacca
      961 tcgcccaggt cgagacaccc agaaccagga gcagcgccgg cgtggcgaac cttcttgccc
     1021 ggatctccat accgaaatta ttgcccgaag tgtcggctct ttggcaaaga agagccccgg
     1081 atctctccgg ggctctcttc gaaactgagt tgccggggcc tagcgcgagc gttcagcatc
     1141 caaggtcctg agccaatctg cgcccgatct ggcggtgccc tgtgactgat cgacagtgct
     1201 aggcacggcg aatggtcaca ggtaccgcca ggcgggacgc gaatcgactc agccgcagcc
     1261 ggtgttgttg ccgcagctcg agcaggtgta acaggcgccg gtgcgctgca tcatgcctcc
     1321 gcactggtcg caggccgggc ctagctcggc cgccccgggg gtgaagtgga gcttggccgg
     1381 aaccaccggg acgtcatcgg tcagggccgt gccggccggg accgaggtgc ctccggtgtc
     1441 ggccaccggg gcctccgcct ccggggccgg gccaccgttg ccgccgtttc cgttcccgcc
     1501 gtttcctgcc gggctgccgt tcccgcttcc gttggccagg ttgatcccgg cgttctcggc
     1561 ttccttccgg ttgcggacct cctgggtcat gatcccctgc tcctcgagga agtcgggatc
     1621 atcgatgaac tgggaggcca gccagcgcat gatgtagtcc ggcatcgact tggcgaaggg
     1681 gatgtccggg ttgccggtga tcccctccgg gtcgaagcgc atgtagctga acttgttgac
     1741 gaacacctcg agcggcaccc cgtactggag gccgagggag atcgcggtcg cgtaggcatt
     1801 catcatgccc cgcatggtcg agccttcctt gccgatgtcg gtgaggaaga tctccccgac
     1861 cgaaccgtcc gcgtacttgc cggccgtgat gtagccctcg tggccgccga ccgagaactt
     1921 gtgggtcacc gaatcccggg tcttcggcat ccggtgccgg accggaccgg ccggagcagc
     1981 ctcggctgcc gcttcctttt ccttctcctt gtccttctgg gcgtcggtgc gaagggcctg
     2041 ggcggtcttt gaaccgtccc ggtagatcgc cagcgccttc aggccgcctt cccagccacg
     2101 ggtgtaggcg tcggcgatgt cctcgacggt cgccgtgttg ggcatgttga ccgtcttcga
     2161 gatcgctccg gagaggaagg gctgggtcgc ggccatcatg tcgatgtggc cggtgtggga
     2221 gatcgcccgc tccccgaccg ccacgtcgaa gaccggcagg tgctcgtcct tcagtccggg
     2281 ggcgccgatg atcgtgccgt gctcgttgat gtaggcctcg atctggccga tctccgactc
     2341 cgagtagccc agcgtctgga gggccagtgg caccgacttg ttgacgatgg tcatgttgcc
     2401 gccgccgacc agctccttga acttgaccag cgagaagtcc ggctcgatgc cggtcgtgtc
     2461 gcagtccatg aggaagctga tcgttccggt cggggccagc accgtcgcct gcgcgttgcg
     2521 gtagccgtgt tcttcgccgg tcgcgaccgc gacatcccac tcttcccggg ccgcgctgag
     2581 cagctcctcg tggacgccct cgccgttgat ctcgtaggcc gcgtcgcggt gcatgcgcat
     2641 cacgttgttg tgcggctcgc ggttcagctc ataggcctcg taggtgccca tcgtcgcggc
     2701 gatctcggcc gagcgccggt aggcgcggcc ggtcatcagc gaggtgatcg cggcggcgac
     2761 gttgcggccc tcttccgagt cgtagggcat gccatccgac atcagcaggg cgccgaggtt
     2821 ggcgtagccg aggccgagct gacggaaggc cctggcgttc ttaccgattt cctcggtcgg
     2881 gtagctggac ggcccgacga tgatctcctg ggcgagaaag accgtgtcga ccacggcgca
     2941 gaagccgtcg acgtcgaagc tgccatccgg gcggcggaac ttcatcaggt tgatcgaagc
     3001 caggttgcag gccgaatcgt cgacatgcat gtattccgag caggggttgg aggcgttgat
     3061 ccggcccgac tccgggcagg tatgccactt gttgatgatc gtgtcgtact ggacgcccgg
     3121 atcggcgcag tcccaggcgg cttcggcgat cttgttcatc agctcccggg cggagagggt
     3181 cttggtgacc gaaccgtcgg tacgggcgag caggttccac tcggcgtcgt cggcgaccgc
     3241 ctgcatgaac tcgtccgaga cccggaccga gttgttggcg ttctggtact ggatcgaggt
     3301 gaagccgtca ccgtcgatcg acatgtcgaa gccggcgtcg cgcagggcgg ccgccttctt
     3361 ctcctcgtcg gccttgcagg tgatgaagtg ctcgacgtcg ggatggtcga cgtcgaggac
     3421 caccatcttc gccgcccgcc gggtcttgcc gccggacttg atggttccgg cccaggagtc
     3481 ggcgccgcgc atgaaggaga ccgggccgga ag
//
LOCUS       DAOTIP010000124         3511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31628_length_3511_cov_3.600116, whole genome shotgun sequence.
ACCESSION   DAOTIP010000124 DAOTIP010000000
VERSION     DAOTIP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31628_length_3511_cov_3.600116"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1570)
                     /locus_tag="P5138_03355"
     CDS             complement(23..1570)
                     /locus_tag="P5138_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59642.1"
                     /translation="MKKFVLGMILSLSMLGAADADAVPKYNASCMFNGCGGVSDEGER
                     AIFTFDDMYPGPDADFDIYQRVNGETSKLIDYPEGKYRQAQLEFVSDNARRAIVETRS
                     PLTPDDTDGWGNDFFAVQDGVPELISWDPADPSTKSENIDLQLIDATDDGRTLYFYRF
                     PPGFPPCIQVWKRTEDAFTQVPTPNCWDNRIFGVSKDGGSTFMGGLESPWRFKDGETT
                     PVNDIPYTGGSHCTKYTTFGDSSPDGETWVFSTDQQLVASDTDGDFDIYMRHEDGSYE
                     MLTDENHGASEENCGDRDFETIPVGLSSDQSEVLYVTHLPVSPEDEDSELDVYLHTPQ
                     GDELISTGPEDDSPEVRNPVVTHRQARRPESQYRVDGSQDLSVIAFDSEQALVPEDTD
                     DDIDVYAWIDGQIRLISTGPNPVETSRKAKLLGVSNDGTTISFSTPEPLLPVDTDLRM
                     DIYAFSTDGLYRPGDAPASASASARKKKRRVVLVSAESIAPRMKVIGRPRAAGRRAVV
                     RLRCRQR"
     gene            complement(1663..2208)
                     /locus_tag="P5138_03360"
     CDS             complement(1663..2208)
                     /locus_tag="P5138_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59643.1"
                     /translation="MTRSAVHRLGIALVFFSALYWLSDLIEAVQGGFSDWQLVITLIA
                     EAAIPFMVIGVYLVQRPRIGLLGLVSSIGYAYAFVFFTGTVVYALVDGTPDYDTMVDQ
                     IGVAMTLHGAIMVLAGIGLGIAVTRAGVLPAWTGVMLGLGVVAVAATQGAGEVIGLVA
                     AGIRDLAFAGMGLAVLTSVDS"
     gene            complement(2229..3101)
                     /gene="gluQRS"
                     /locus_tag="P5138_03365"
     CDS             complement(2229..3101)
                     /gene="gluQRS"
                     /locus_tag="P5138_03365"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRV59644.1"
                     /translation="MGDLDLFHADGDPGKAPGRYAPSPTGRLHLGNLRTALLACRAAR
                     VREAPFLLRIEDVDTGRSREEWIEVQLGELRQIGVDWDGEPVRQSDRHDLYRGALDRL
                     EADGLLYECFCTRAEIREAASAPHGELPEGAYPGTCRELTESEREERRQAGRPPALRV
                     RAAGVKVGFENHLCGTVEKTLDDFVVRRNDGDFAYNLAVTVDDAEQGIGEVVRGQDLL
                     DTTPRQLWLYNRLGLAAPARFAHVPLMLGDDGERLAKRHGAITLEERIESGEDPSAVR
                     RGLVESVEGILGPG"
     gene            complement(3110..>3511)
                     /locus_tag="P5138_03370"
     CDS             complement(3110..>3511)
                     /locus_tag="P5138_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRV59645.1"
                     /translation="PAWLHPEARRPRRVNARALLAPFDPLIFERRRLEELFDFHYRIE
                     IYTPKAKRRHGYYVLPLLLNEEIVGRVDLKSDRDGSTLLVQSAWVEPEAPVNTAEELA
                     GELRLMSGWLGLDEVEVRPHGDLADDLKATL"
BASE COUNT          616 a         1142 c         1124 g          629 t
ORIGIN      
        1 tgctgctcct cctttttgtc tactacctct ggcggcaacg cagcctgacg accgctcggc
       61 gtccggcggc acggggcctg ccgatcacct tcatccgggg agcaatcgat tccgccgata
      121 cgagcaccac acgacgcttc ttcttccggg ccgacgccga agcggatgcc ggcgcatcac
      181 ccggccggta gaggccgtcg gtcgagaacg cgtagatatc catccggagg tcggtgtcga
      241 ccggaagcag cggctcgggt gtgctgaacg agatcgtcgt gccgtcattg gacacgccca
      301 gaagcttggc cttgcgcgag gtctcgaccg ggttcggccc ggtcgagatg aggcggatct
      361 gaccgtcgat ccaggcgtag acgtcgatgt cgtcgtcggt gtcctccggg accagggcct
      421 gttcggaatc aaaggcgatc acggagagat cctgcgatcc gtccacccgg tattgcgact
      481 ccgggcgacg cgcctgacga tgggtcacga cgggattgcg tacctccggg ctgtcgtcct
      541 ccggtccggt cgagatcagc tcgtctccct gcggggtgtg caggtagaca tccagttccg
      601 aatcttcatc ctcgggtgag accgggagat gcgttacgta gagcacctcg ctctggtcgc
      661 tggaaagacc gacggggatg gtctcgaaat cacggtcacc gcagttctcc tccgatgccc
      721 cgtggttctc gtcggtgagc atctcgtacg aaccgtcctc gtgtcgcatg tagatgtcga
      781 agtccccgtc cgtgtcggaa gcgacgagtt gctggtctgt cgagaaaacc caggtctcgc
      841 cgtcggggct cgagtctccg aaggtcgtgt acttggtgca gtgcgagcca cccgtgtaag
      901 ggatgtcgtt cacgggggtg gtttccccgt ccttgaaacg ccagggggat tcgaggcctc
      961 ccatgaaggt gctcccgccg tccttggaca caccaaatat ccggttgtcc cagcaattcg
     1021 gggtcgggac ctgcgtgaat gcgtcttcgg tccgcttcca cacctgtatg cacggcggaa
     1081 aaccgggggg gaaacggtag aagtagaggg tgcggccgtc atcggtggcg tcgatcagct
     1141 gcaaatcgat gttctccgat ttggtcgagg ggtcggccgg atcccaggag atgagctcgg
     1201 gcacgccgtc ctggaccgca aagaagtcgt tcccccagcc atcggtgtca tcgggagtga
     1261 gaggggaacg cgtctcgacg atcgcccggc gggcgttgtc ggagacgaac tcgagctgcg
     1321 cctgacggta cttcccttcg gggtagtcga tcagcttgct tgtttcgccg ttcacccgct
     1381 ggtagatgtc gaaatccgcg tcgggaccgg ggtacatgtc gtcaaaggtg aagatcgcgc
     1441 gctcgccctc gtccgagacg cctccgcatc cgttgaacat gcagctcgcg ttgtatttcg
     1501 gcacagcatc tgcgtcggct gcgccgagca tggagaggct gaggatcatc cccagaacga
     1561 acttcttcaa gatcttgctc cctgtctgga gcactccggc tcccatcgag tgactacagg
     1621 ctaggcgaaa accctgacat aggccgaaag gtgcgattcc ggtcaggaat cgaccgaggt
     1681 caagacggcg agacccatcc ctgcgaaggc cagatcccgg attccggcgg ccaccaggcc
     1741 gatgacctcg ccggcgccct gtgtcgccgc gaccgccacg actcccagac caagcatcac
     1801 accggtccag gcgggcaata ctcccgcccg ggtcacggca atgcccagac cgatcccggc
     1861 cagcaccatg atcgctccgt gaagcgtcat cgcgacaccg atctgatcga ccatcgtgtc
     1921 gtagtcgggc gtcccgtcca ccagggcgta gacgaccgtt ccggtgaaga acacgaaggc
     1981 gtacgcgtag ccgatcgacg aaaccaggcc aaggagtccg atccggggcc gctgaaccag
     2041 atagaccccg atcaccatga agggaatcgc cgcctccgcg atgagggtga tcaccaattg
     2101 ccagtccgag aagccaccct ggaccgcctc gatcaggtca ctcagccagt agagagccga
     2161 gaagaatacg agggcgatac ccagcctgtg aaccgcggat cgggtcatgt ccggattcta
     2221 ggaatctgtc aacccggacc aaggatcccc tcgacggact cgaccagacc cctccgtacg
     2281 gccgacggat cctctcccga ctcgattcgt tcttccaggg tgatcgcgcc gtggcgtttg
     2341 gcgagacgct cgccgtcatc gccgagcatc agggggacat gggcgaacct ggccggggcg
     2401 gccagtccga ggcggttata gagccagagc tggcgggggg ttgtgtcgag gaggtcctgg
     2461 ccccggacaa cctccccgat tccctgttcg gcgtcatcga ccgtgaccgc gaggttgtag
     2521 gcgaaatccc cgtcgttacg ccggaccacg aagtcatcca gcgtcttctc gaccgtgcca
     2581 cagagatggt tttcgaagcc gaccttcaca ccggcggccc ggaccctcag ggccggcggc
     2641 cttcctgcct gccgccgctc ctcccgctcg ctttcggtca gctcacggca ggttccggga
     2701 taggcccctt ccggcaactc gccgtgcggg gccgaggcgg cttcacggat ctccgcccgg
     2761 gtgcagaaac actcgtagag gagtccgtcc gcttcgagcc ggtcgagggc tccgcggtaa
     2821 aggtcatgtc gatcgctctg gcggaccggc tcgccgtccc agtcgacccc gatctgccgt
     2881 agctccccga gctgaacctc gatccactcc tcccgggagc ggccggtatc tacgtcctcg
     2941 atccggagca ggaagggggc ctcccggacg cgagccgccc ggcaggcgag cagggccgtc
     3001 ctcaggttgc cgaggtggag acggccggtc gggcttggtg cgtaacggcc gggcgccttt
     3061 ccgggatctc cgtcagcgtg gaacagatcg aggtccccca cgttcgccgt caaagcgtcg
     3121 ccttcaggtc atcggcaagg tcgccgtgcg gccggacctc gacttcgtcc aggccgagcc
     3181 agccggacat cagcctgagc tcgccagcca gctcctccgc cgtgttgacc ggcgcctccg
     3241 gttccaccca ggccgactgc acgagcaggg tcgatccgtc acggtcggat ttcaggtcga
     3301 cccggccgac gatctcttcg ttcagcagca gcggcagcac gtagtaaccg tgacggcgtt
     3361 tcgccttcgg tgtgtagatc tcgatccggt agtgaaagtc gaacagctcc tccagccggc
     3421 gccgctcgaa gatcagcgga tcgaagggcg cgagcagggc ccgggcattg actctgcgag
     3481 ggcgcctggc ctcgggatga agccaggcgg g
//
LOCUS       DAOTIP010000125         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31652_length_3509_cov_3.367690, whole genome shotgun sequence.
ACCESSION   DAOTIP010000125 DAOTIP010000000
VERSION     DAOTIP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31652_length_3509_cov_3.367690"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="P5138_03375"
     CDS             <1..1016
                     /locus_tag="P5138_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRV59646.1"
                     /translation="ERVVISGEEEPALFADCVRRTSEAAATVNTRLEDEEVTQFELAT
                     AAAFLALAESGVQVAAIEAGLGGRLDATNSIDSLATVLTSIGLDHTEYLGETEVEIAG
                     EKLAVLRPGTTLVLGPVSAEVLELARTTAEERGCELLEITGAAAAGAAGVHGIGGFQR
                     TNFAVAEAAAACFLGEVSRHKAEEAVASLVIHGRLEQIGSDPLVLADVAHNPAGARAL
                     ASSLPEVAGDLPVIGVIGMLADKDAPGMLSELAGSLDAAVFTRLPREALEAWGRPGAR
                     GWEPDELMRLASGLGLRSESAPTPLDALARAREMALERGGAVLIAGSHFLFAPGEGAD
                     VPA"
     gene            1213..2028
                     /locus_tag="P5138_03380"
     CDS             1213..2028
                     /locus_tag="P5138_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRV59647.1"
                     /translation="MPLAIFGIENTALNLAATLVILMLVAIWIALIVFTFTDARRRIA
                     DPFLVGCATAASFFPFVGTLVYTILRPPEFLEDIRERETDINESEVRLRHLKHNSCHK
                     CGSPVESDFIRCPACRTRLKEPCPTCSKPVGLNWKVCPYCEKTLIAPKRSSRSGSSGR
                     SKPGRTERSPEKDERRRRSSKAKAETSSGESRSESRSSSRESSSREGRSSSREGSSSR
                     EGSSRENRSEEPTRRTKSSSSEKEESPRTPRSARRKITVGDEETSEPINRGDN"
     gene            2096..2503
                     /gene="ndk"
                     /locus_tag="P5138_03385"
     CDS             2096..2503
                     /gene="ndk"
                     /locus_tag="P5138_03385"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRV59648.1"
                     /translation="MSQTLILVKPDAFERSLTGEVLARFERKGLRIAALKKMTATEQI
                     ANEHYAEHQEKPFFGELVDFITGGPLVAAVLEGPEAVVAARQLIGATNPVEAAPGSIR
                     GDFALEVTFNMVHGSDSDESAAREIAIWFPEGV"
     gene            complement(2553..3329)
                     /locus_tag="P5138_03390"
     CDS             complement(2553..3329)
                     /locus_tag="P5138_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRV59649.1"
                     /translation="MDELVDSLRAAGSVFAEDEARILAESADSPAELDSMLRRRMEGE
                     PIQQIVGWAEFCGMRIRVEPGVFLPRPRSEFLVGKAIGLGRPGCTILDVCCGTGALGV
                     AAATGLGSADLHATDLDPAAVRCAAVNVDEVAGRVYLGDLFGPLPRSLRGRADLLLLN
                     APYVPTAELPLMPREARLHEERITLDGGEDGLDVQRRAILEAPLWMAPGAHLLVETSD
                     IQAESTMRLMKLAGLAVTLATSNEWGATVVIGRKADSDQR"
BASE COUNT          595 a         1176 c         1162 g          576 t
ORIGIN      
        1 cggaacgggt ggtgatctcc ggggaggaag aaccggcgct tttcgccgac tgcgttcgtc
       61 ggacctccga ggcagcggcc accgtcaaca cccggctgga ggacgaggaa gtgacccagt
      121 tcgagctggc aaccgcagcc gcgtttctgg ccctggccga atccggagtc caggtcgcgg
      181 cgatcgaagc cggccttggc gggcggctcg acgcgaccaa ctcgatcgat tccctggcga
      241 ctgtcctcac ctcgatcgga ctggatcaca ccgaatacct gggcgaaacc gaggtcgaga
      301 tcgccgggga gaaactggcc gtgctccggc ccggcacgac acttgtcctc ggtccggtgt
      361 cggcagaagt cctcgagttg gcgaggacga ccgcggagga gcggggttgc gagctactgg
      421 agatcaccgg cgcggccgcg gcaggagcgg ccggtgtcca cggaatcggg ggcttccagc
      481 gaaccaactt cgcggtcgcg gaagcggcgg cggcatgttt cctcggtgag gtttcacgcc
      541 acaaggccga ggaggcggtc gcatcgctgg tcatccacgg gcgactcgag cagatcggca
      601 gcgacccgct cgtcctggcc gacgtcgccc acaacccggc cggtgccagg gccctggctt
      661 cgtccctgcc cgaggtggcc ggcgatctgc cggtgatcgg ggtgatcggg atgctggccg
      721 acaaggacgc cccggggatg ctgtccgagc tggccggatc actggacgcg gccgtcttca
      781 cccggctgcc acgggaggcg ctggaggcat ggggaaggcc cggcgcccgg ggctgggagc
      841 cggacgaact gatgcgcctc gcttccgggc tgggacttcg gtcggagtcc gctccgactc
      901 ctctcgacgc gcttgcgagg gcgcgggaaa tggctctgga aaggggcgga gcggttctga
      961 tcgccggctc ccattttctc ttcgcgcccg gggagggagc cgacgtgccg gcctgagggc
     1021 cgggcaaggt ctctcagaac ccggaccggg gggttcggca gccgccggtt atgtcacgat
     1081 tacccggtga atcgtcagac tggctcccaa ttgctgtcga tgatggcgct ggtcgctgcg
     1141 gtcgtggcga tcgtgatcct cgtcttcttc ggcatcgggt acctattcgg aaggatgttc
     1201 ctctagtcag ccatgcctct cgccatcttc ggaattgaaa acaccgcact gaacctggcc
     1261 gccacgctgg tgatcctgat gctggtcgcg atctggatcg ccctcatcgt gttcaccttc
     1321 accgatgccc gccgccgaat cgccgacccc ttcctggtcg gctgcgccac cgcggcgtcg
     1381 ttcttcccct tcgtcggcac gctcgtctac acgatcctcc gcccgccgga gttcctcgag
     1441 gacatccgcg agcgcgagac ggacatcaac gagtccgagg tccggctcag gcatctcaag
     1501 cacaactcct gccacaagtg cggctcgccg gtcgagagcg acttcatccg ttgccccgcc
     1561 tgccggaccc ggctcaaaga gccctgcccg acctgctcca agccggtcgg actcaactgg
     1621 aaggtctgcc cttactgcga gaagaccctg atcgccccga agaggtcttc caggagcggc
     1681 tcttcgggcc gttccaagcc gggacgaacc gaacgcagtc ccgagaagga cgagcgtcgc
     1741 cgtcgctcga gcaaggcgaa agccgagacc tcgtcgggcg agagccgttc ggaaagccgt
     1801 tcttcctccc gggagagttc ttcccgcgag ggacggtcgt cgtcgcgtga gggatcttca
     1861 tcgcgtgagg gatcctctcg cgagaaccgt tcggaagagc cgacccggcg taccaagagc
     1921 tcttcgtcag agaaggaaga atcgccccgg acgccgcgtt cggcccggcg caagatcacg
     1981 gttggcgacg aggagacctc cgagccgatc aaccgcggcg acaactgacg cccgtcgcgg
     2041 gtactctgac ccgccggctt ccgccacaac caaccccaca acggagtaac tagacatgtc
     2101 ccagaccctg atcctggtca agcccgatgc cttcgaacgt tcgctcaccg gcgaggtcct
     2161 cgcccgcttc gagcgcaagg gcctccgcat cgccgccctc aagaagatga ccgccaccga
     2221 gcagatcgcc aacgagcact acgcagagca ccaggaaaag cccttcttcg gcgaactggt
     2281 cgacttcatc accggcggac cgctggtggc tgccgtcctc gaagggcccg aggccgtggt
     2341 cgctgcccgc cagctgatcg gcgccaccaa cccggtcgag gccgctcccg gttcgatccg
     2401 cggcgacttc gccctcgagg tcaccttcaa catggttcac ggctccgact cggacgagtc
     2461 cgccgctcgc gaaatcgcga tctggttccc cgagggagtc tgaacatcgg ccggtgatgc
     2521 aggtttgcga ccgtccaggt agcaaacctg cgtcaccttt ggtcagagtc ggcttttctt
     2581 ccgatcacga cggtggctcc ccattcgttt gaggtggcca gagtcaccgc gagtcccgcg
     2641 agcttcatca ggcgcattgt cgattcagcc tggatgtcgc tggtttcgac cagaaggtgt
     2701 gcgccggggg ccatccagag cggggcttcc agaatcgccc ggcgctggac gtcgaggccg
     2761 tcctcgcccc cgtcgagggt gattctctct tcatggagcc gggcttccct gggcatcagc
     2821 ggcagctcag ccgtcggcac gtaaggcgcg ttgaggagca ggagatcggc ccggccccgg
     2881 agggaacggg ggagagggcc gaagaggtcg ccgaggtaga cccttcctgc gacctcatcg
     2941 acgttcacgg ccgcgcagcg cacggcggcg gggtcgaggt cggtggcgtg gaggtctgcc
     3001 gagccgaggc ccgtcgcggc cgctacgccg agagcgccgg ttccgcagca gacatcgagg
     3061 atggtgcagc cgggccggcc gagcccgatc gccttcccga ccaggaactc ggaacgcggc
     3121 cgagggagga agaccccggg ttcgacccgg atcctcattc cgcagaattc ggcccagccg
     3181 acgatctgct ggatcggctc gccctccatt cgccggcgca gcatcgaatc cagttcggcc
     3241 ggtgaatcgg cggactcggc gagaatgcgc gcctcatcct cggcgaaaac cgagcccgcg
     3301 gcccgcagtg aatcgaccag ttcatccatc gcagccaata ttgaacggtg ggtcggattt
     3361 ccggcccccg cgccggaaat ccgacccacc ggggagtggc cgaggtgcgg gcataggatt
     3421 cacatgtggg tgtttccgaa aaagtccgcc gccaggatgc gattgaggcc gcgcgaaggc
     3481 actacctcga ctgcgagctg atagacatg
//
LOCUS       DAOTIP010000126         3497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31794_length_3497_cov_3.424463, whole genome shotgun sequence.
ACCESSION   DAOTIP010000126 DAOTIP010000000
VERSION     DAOTIP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31794_length_3497_cov_3.424463"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="P5138_03395"
     CDS             complement(<1..1547)
                     /locus_tag="P5138_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRV59650.1"
                     /translation="MAERDYLSAISDHIVVFDGGMGATLEMFDLTQEDYGGLLGKCHE
                     ALVLNRPDVIEGVHASMLEAGAEVVETDTFQASRVKLDEWGLADHTEEINVKAAEIAR
                     KAAGPDRFVAGSIGPTGHLPASDDPTLGQIRFPELVTVFTEQAGGLVRGGADLIIIET
                     AQDILEVKAAIFGARVAFEEVGREVPIQISVSLLPNGGKMLLGTDIQAVLTTLEALKV
                     DVIGLNCSTGPEDMRDAIRYLGENSTVPVHCIPNAGLPLQGPDGETIFPEKPEPLAAT
                     LGEFVERYGVGVVGGCCGTTPEHIKAIADRVRDLKPGPRPGPGEIKVSSMMTSTALVQ
                     EPTPTMVGERVNSQGSRKAKELLLADDYDGLLQVAEDQVEGGAHVLDVCVALTERQDE
                     DEQMRQVVKRISLTQPSPIQIDSTEPNVIRTALEQIPGRAIVNSVNLEAGRDKLDTVA
                     PLAKAHGACLIALTIDEVGMAKTADRKVEIAKRITEMVVDEHGLDRESLIFDDLTFTL
                     TTGDEEWR"
     gene            1617..2135
                     /locus_tag="P5138_03400"
     CDS             1617..2135
                     /locus_tag="P5138_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HRV59651.1"
                     /translation="MDGATAYIALLRAVNVGGTGKLPMAELRKLAEEIGFAGVSTYIA
                     SGNLLFKSDLTEKQVKKQLEAALKRHTGMTIDVIVRTAREMRAVELANPFKGVAYNRI
                     GVLFLDHPLPRDNIRNTKGRKNEEIELGKREVYIHYPDGMGKSKLNIPAAKYGTSRNL
                     NTVSKLADLAKG"
     gene            complement(2183..2908)
                     /locus_tag="P5138_03405"
     CDS             complement(2183..2908)
                     /locus_tag="P5138_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HRV59652.1"
                     /translation="MDEPIPTQQHPDEPHSAGISQRLNWLRAGVLGANDGIVSTASLV
                     VGVAGATTDTTAILIAGVAGMVGGAVSMALGEYVSVSSQRDSERALVVKERGELTDFP
                     DAELKELEYLLRQRGISEQTARQVALELTDHDPLAAHLEFELGIREDDFANPWHAAIS
                     SALSFTAGAILPLVAILLPPPDLRVPITFVSVLLTLALTGAVSAEIGGSSKRLAATRL
                     VIGGALALLATWLIGTLLGTTVG"
     gene            complement(2961..>3497)
                     /locus_tag="P5138_03410"
     CDS             complement(2961..>3497)
                     /locus_tag="P5138_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV59653.1"
                     /translation="ESLARAIADLGGSFIVASGQLPDLSPGNVLLAAEGGLVRATAVT
                     SDRGFDGGDRPDRDGLAELADRFRPGFAGLLEPTVNWLDLQGLRPAKTLWHAAADRLA
                     QSLVWSGKAFDRPDLALELTRLTVGPESPDPRLAIPVERAEDDWGEEYHLRSTCCLAY
                     RTGGGELCQSCPLVKDRS"
BASE COUNT          586 a         1106 c         1219 g          586 t
ORIGIN      
        1 cgccactctt cgtcaccggt ggtcagggtg aaggtgaggt cgtcgaagat cagcgactcg
       61 cggtcgagtc cgtgttcgtc cacgaccatc tccgtgatcc gtttggcgat ctccaccttg
      121 cggtcggcgg tcttcgccat acctacctcg tcgatcgtca gggcgatcag gcaggcgcca
      181 tgggccttgg ccagcggggc cacggtgtcg agcttgtcgc ggcccgcttc gaggttgacc
      241 gagttgacga tcgcccggcc cgggatctgc tccagggctg tcctgattac gttcggctcg
      301 gtcgagtcga tctggatcgg ggagggctgg gtcagcgaga tccgcttaac cacctgcctc
      361 atctgctcgt cctcgtcctg gcgttcggtc agggctacgc agacgtcgag cacatgggct
      421 ccgccttcga cctgatcctc ggcgacctgg agcaggccgt cgtagtcgtc ggccagaaga
      481 agttccttcg ccttgcgcga gccctgcgag ttgacccgct cgccgaccat ggtcggggtc
      541 ggctcctgga ccagggcggt ggaggtcatc atcgaggaga ccttgatctc gcccggcccg
      601 ggacgggggc caggcttcag gtcacggacc cggtcggcga tcgccttgat gtgctcgggg
      661 gtggtgccgc agcagccacc gaccacgccg accccgtagc gctcgacgaa ctcgcccagc
      721 gtcgcggcca ggggttcggg cttctcgggg aagatcgtct cgccgtccgg accctgcagg
      781 ggcaggccgg cattggggat gcagtggacc ggaacggtcg agttctcgcc gaggtagcgg
      841 atcgcgtcgc gcatgtcctc ggggccggtc gagcagttca ggccgatcac atcgaccttg
      901 agcgcctcga gagtggtcaa cactgcctgg atgtcggtgc cgagcagcat cttgccgccg
      961 ttcggcagga gcgaaacgga aatctggatc gggacctcgc ggccgacctc ctcgaaggcg
     1021 acccgggcgc cgaagatcgc ggccttgact tcgaggatgt cctgggcggt ctcgatgatg
     1081 atcaggtctg cgccgcccct gaccagaccg ccggcctgtt cggtgaagac ggtgaccagt
     1141 tcggggaagc ggatctggcc gagggtgggg tcatccgagg cgggcaggtg cccggtcggc
     1201 ccgatcgaac cggcaacgaa acggtccggg ccggccgcct tgcgggcgat ctcggccgcc
     1261 ttgacgttga tctcttcggt gtggtcggcg aggccccatt cgtcgagctt tacccgcgag
     1321 gcctggaagg tgtcggtctc gaccacctcc gctccggctt cgagcatgga ggcgtggaca
     1381 ccctcgatca cgtcgggacg gttgagcacc agcgcctcgt gacacttgcc gagcaggccg
     1441 ccgtagtcct cctgggtcag gtcgaacatc tccagggtcg cgcccatgcc gccgtcgaag
     1501 acgacgatgt ggtcggagat tgcgctcagg tagtcacgtt cagccatcgg tcgaggatac
     1561 cgagccggta aatccgatca ggattcgggg gttggccagt gtcctagtct ttgtccatgg
     1621 acggagccac cgcttacatc gcattgctca gggcagtgaa cgtggggggt accggcaagc
     1681 tgccgatggc cgagctacgg aagctcgccg aggaaatcgg gttcgcggga gtcagcacct
     1741 acatcgccag tggaaacctc ctcttcaagt cggatctgac cgagaagcag gtgaagaagc
     1801 aactagaggc tgcgctgaaa aggcacacgg ggatgaccat cgacgtcatc gtcaggacag
     1861 cccgggagat gcgcgcggtc gagctggcga acccgttcaa gggagtggcc tacaacagga
     1921 tcggcgtgct cttcctggat cacccgttgc cgcgggacaa catcaggaac acgaaaggcc
     1981 gcaagaacga ggaaatcgaa ctcggcaagc gcgaggtcta catccactac ccggacggca
     2041 tgggcaaatc gaagttgaac atcccggccg cgaagtacgg caccagccgc aacctgaaca
     2101 ctgtctcgaa gctggccgac ctggccaaag ggtgagcggc aggcagcggc gggtcagacc
     2161 gccgtggtac ccggcggaag ggtcagccca ccgtggtgcc cagcagggtt ccgatcagcc
     2221 aggtggccag cagggccagc gcccctccga tcaccagccg ggtggcggcc agccgttttg
     2281 acgatccacc gatttcggcg gagaccgcgc cggttagcgc gagggtgagc agaacgctca
     2341 caaaggtgat tggaacccgg aggtcgggcg gggggaggag gatcgcgacc aggggaagga
     2401 tcgccccggc cgtgaaggac agcgccgagg agatggccgc gtgccagggg ttggcgaagt
     2461 cgtcctcgcg gatgcccagt tcgaattcga ggtgggctgc cagcgggtcg tgatcggtca
     2521 attcgagggc tacctggcgg gcagtctgct cggaaatgcc tctctggcgc agcaggtatt
     2581 cgagttcctt gagctccgcg tccgggaagt cggtgagctc gccccgttcc ttcacgacga
     2641 gggctctttc gctgtcgcgc tggctgctga ccgacacgta ctcgcccagc gccattgaaa
     2701 ccgcgccgcc gaccattccg gccacgccgg cgatcaggat cgctgtcgtg tccgtcgtcg
     2761 cccccgccac gccgacgacc agggaggcgg tcgaaacgat tccgtcgttg gcaccgagaa
     2821 ctccggctcg caaccagttg aggcgctgtg aaatgccggc ggagtgaggt tcgtcgggat
     2881 gctgctgggt cgggatcggc tcatccatcc cggcaaaggc tatctcccga aacgggtggc
     2941 cccgccggaa tcgcggttct tcaactccgg tccttgacca gcgggcagga ctggcagagc
     3001 tcgccgcctc cggtccggta ggcgaggcag caggtgcttc ggaggtgata ctcctcgccc
     3061 cagtcgtcct cggccctttc gaccggaatc gcgaggcggg ggtcggggct ttccgggccg
     3121 accgtgagcc gggtcagctc gagggcgaga tccggccggt cgaatgcctt ccccgaccag
     3181 accagcgact gggcgagccg gtccgctgcg gcatgccaca gcgtcttcgc cggcctgagg
     3241 ccctgaaggt caagccagtt gacggtcggt tcgagcagcc cggcgaatcc ggggcggaac
     3301 ctgtcggcca gctcggccag tccgtcccga tcgggtcggt caccgccgtc gaacccgcga
     3361 tcggaggtga ccgcggtagc ccggacgagt ccgccttcgg cagcgagcag gacgttcccg
     3421 ggcgagaggt ccggcagctg gccggacgca acgatgaacg agccgccgag gtcggcgatc
     3481 gcgcgggcca ggctttc
//
LOCUS       DAOTIP010000127         3486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_31932_length_3486_cov_2.966482, whole genome shotgun sequence.
ACCESSION   DAOTIP010000127 DAOTIP010000000
VERSION     DAOTIP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31932_length_3486_cov_2.966482"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="P5138_03415"
     CDS             complement(<1..248)
                     /locus_tag="P5138_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59654.1"
                     /translation="MWLLPDDRVRARVEKRQYRSVVKHIFVPRRSGWVLRRPDGEAVG
                     AALWYPPGTKKHSFRESIFESLPFLPAGIPHLGKAARL"
     gene            complement(322..612)
                     /locus_tag="P5138_03420"
     CDS             complement(322..612)
                     /locus_tag="P5138_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRV59655.1"
                     /translation="MKLYVCGQGTSGPAAMHPCAKAGKALDEAGYTYELEKVGGYRML
                     PWTWRTRAADRKKIKEISGTNEVPVLVLDDGEVISDSGAIARWARENPAPGS"
     gene            complement(632..1915)
                     /gene="purB"
                     /locus_tag="P5138_03425"
     CDS             complement(632..1915)
                     /gene="purB"
                     /locus_tag="P5138_03425"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRV59656.1"
                     /translation="MIERYTRPEMGAVWTQQAKFQSWLDVELAATDAWAAEGVVPSEA
                     ATACREKGAFTVEEVNAREEITNHDVAAFVDVVAASIGEEGRWIHFGLTSSDVLDTAL
                     AVQLTKAGEILVAGAKTYRDALIERAFEFRDTLAMGRTHGVHAEPTTFGLRLAGFAFE
                     ADRNWKRLQQATAQAARGKLSGAVGTYASVPPTIERRVMDTLGLQREEIATQVVPRDR
                     HAELLSAIALAGAGIERFATEVRHLQRTEVREAEEPFATGQQKGSSAMPHKRNPIRTE
                     RMTGLARLLRGYAQTGLENVALWHERDISHSGAERVILPDATICLDYMHHVITQVASG
                     LVVHVDRLQSNLEQTNGAMFSQRALTALVESGMTRDDAYRTVQAAAHEAFDGGPHFRE
                     LIGKAAPELDLDEIFDYGAYLTHLPEVFERLEEIR"
     gene            complement(1922..2395)
                     /gene="purE"
                     /locus_tag="P5138_03430"
     CDS             complement(1922..2395)
                     /gene="purE"
                     /locus_tag="P5138_03430"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRV59657.1"
                     /translation="MSEETQQPATLSSVEELFEEIEVEQPQVGIIMGSKNDKPKMQPA
                     GQALEDAGIAYEVRVMSAHRDPDLVVDYCTNAKMRGLKVIIAGAGMSAALPGVAAAHT
                     ELPVIGVPILGKSLGGLDALLSAVQMPPGVPVACVAIDGAKNAGVLATRIINLAG"
     gene            complement(2392..>3486)
                     /gene="purD"
                     /locus_tag="P5138_03435"
     CDS             complement(2392..>3486)
                     /gene="purD"
                     /locus_tag="P5138_03435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRV59658.1"
                     /translation="NLVVVGTEAPLVAGVVDALTEAGIAAFGPSGAAAQLEGSKAYAK
                     EAMEEAGVPTAGYGVVTDRDSALAKVAETGCPVVLKADGLAAGKGVLICMDEAEAREA
                     IEVFFTEQRFGQTEVVVEEFLEGEELSLLAICDGERAIALAPAQDYKRIGEGDTGPNT
                     GGMGSYSPVQTVSKDQLADLSALVHQPIVDLMARRGHPFHGVLYAGLMMTADGPKVLE
                     YNTRFGDPETQAVLPRLESDLVDLFLAASKPGGLADKEVSFADIFAVTVVLASAGYPA
                     SSSKGDVITGLEQAAGSAEVVHAGTAEENGQIVTAGGRVLNLTGVGTDAATARDRAYD
                     AADAVNFDGKQMRRDIAERAVRRERDQEAT"
BASE COUNT          500 a         1176 c         1188 g          622 t
ORIGIN      
        1 tgaagacggg cagcttttcc gaggtggggg attccggccg gcaggaaggg caggctttcg
       61 aagatcgact ccctgaagct gtgcttcttg gtgccgggcg ggtaccagag cgccgccccg
      121 accgcctcgc cgtcgggccg gcgcaggacc cagccggatc ggcggggcac gaagatgtgc
      181 tttacgacgg accggtactg gcgcttttcc accctggccc ggacccggtc gtccggcagc
      241 agccacatcc agatctcgtt gtcctgaaat ccgcgggcga ttgcatccgc cgcgtccttc
      301 gcgtcggatg gcccgatcag ttcaggaacc gggggcaggg ttctcccggg cccacctggc
      361 gatcgcgccg gagtcgctga tcacttcacc gtcgtcgagt acgagaaccg gcacttcgtt
      421 ggtgcccgag atctccttga tcttcttgcg gtctgcggcc cgggttcgcc aggtccacgg
      481 caacattcgg tagccgccca ccttctccag ttcgtaggtg tagccggctt cgtcgagcgc
      541 cttgcccgcc ttcgcgcagg gatgcatcgc cgccggaccg ctggtgcctt gaccacagac
      601 gtagagcttc atggccggag cctaacttct cctagcgaat ttcttccaac cgctcgaaga
      661 cctcggggag gtgggtcagg taggcgccgt agtcgaagat ctcgtcgagg tcgagttccg
      721 gggcggcctt gccgatcagt tcacggaagt gcggtccgcc gtcgaaagcc tcgtgggcgg
      781 cggcctggac cgtccggtag gcgtcgtccc gggtcatgcc ggattcgacc agggcggtca
      841 gggcccgctg gctgaacatc gcgccgttgg tctgctcgag gtttgactgc aggcgatcaa
      901 cgtggaccac cagtccggag gcgacctggg tgatcacgtg atgcatgtag tcgaggcaga
      961 tcgtcgcgtc gggcaggatc accctttcgg cgccggagtg gctgatgtcg cgctcgtgcc
     1021 agagggcgac gttctccagc ccggtctggg cgtagccgcg aagcagtctc gccagaccgg
     1081 tcatccgttc ggtgcggatc gggttgcgct tgtgcggcat cgccgacgag cccttctgct
     1141 gaccggttgc gaaaggctcc tcggcctcgc ggacctcggt ccgctgcagg tgccgcacct
     1201 cggtcgcgaa gcgttcgatc ccggctccgg ccagggcgat tgcggagagc agctcggcgt
     1261 gacggtcgcg gggcaccacc tgggtggcga tctcttcccg ctggagtccg agcgtgtcca
     1321 tgacccggcg ctcgatcgtc ggcggcaccg aggcgtaggt gccgaccgcg ccggaaagct
     1381 tgccccgcgc ggcctgggcg gtggcctgct ggaggcgttt ccagtttcgg tccgcctcga
     1441 aggcgaaacc ggccaaacgc aggccgaagg tcgtgggctc ggcgtggacg ccgtgggtgc
     1501 ggcccatcgc cagggtgtcg cggaactcga aggctcgttc gatcagggcg tcgcggtagg
     1561 tcttcgcccc cgcgacgagg atctcgccgg ccttggtcag ctgaaccgcg agggcggtgt
     1621 cgagcacgtc ggacgaagtc aggccgaagt ggatccagcg gccctcctcc cctatcgagg
     1681 ctgccaccac gtcgacgaag gcggcgacgt cgtggttggt gatctcctca cgagcgttga
     1741 cttcctcgac cgtgaacgcg cccttctccc ggcaggcggt tgccgcctcg gacgggacga
     1801 ctccctcggc cgcccaggcg tccgtcgctg ccagctcgac gtccagccag gactggaact
     1861 tggcctgctg ggtccagacc gcgcccatct ccggacgggt gtagcgctcg atcatgcgct
     1921 gctagccggc caggttgatg atgcgtgtgg cgagcacgcc ggcattcttg gcgccgtcga
     1981 tcgccacgca ggcaaccggc acgcccgggg gcatctgcac cgcggaaagc agggcgtcaa
     2041 ggccgccgag cgacttgccg agaatcggca cgccgatgac cggcagctcc gtgtgggcgg
     2101 cggcgacgcc gggcagggcg gcggacatgc cggctccggc gatgatcacc ttgaggccgc
     2161 gcatcttggc gttggtgcag taatcgacca cgagatcggg gtcgcggtgg gcggacatga
     2221 cccgcacctc gtaggcgatt ccggcatcct cgagagcctg tccggcaggc tgcatcttgg
     2281 gcttgtcgtt cttcgagccc atgatgatcc cgacctgagg ctgttcgact tcgatctcct
     2341 cgaagagctc ctcaaccgag gagagggttg ccggctgctg ggtttcttcg ctcatgttgc
     2401 ttcctggtct ctctcgcgac ggaccgccct ctcggcgatg tcccgtcgca tctgtttgcc
     2461 atcgaaattg actgcgtcag ccgcatcgta agcacgatcg cgcgccgttg ctgcgtcggt
     2521 tccgaccccg gtcaggttca gaacccggcc accggcggtg acgatctgcc cgttctcctc
     2581 tgccgtgccg gcgtgaacca cctctgccga cccggccgcc tgctcgaggc cggtgatcac
     2641 gtcgcccttc gaggacgagg ccggataccc cgcggaggcc agcaccacgg tcacggcgaa
     2701 gatgtcggcg aagctgacct ctttgtcggc cagccctccc ggcttcgacg cggcgaggaa
     2761 gaggtcgacg aggtccgatt cgagccgggg caggaccgcc tgggtctcgg gatcgccgaa
     2821 gcgggtgttg tattcgagca ccttggggcc gtcggcggtc atcatcagcc cggcgtagag
     2881 caccccatgg aaggggtggc cgcgacgggc catcaggtcg acgatcggct ggtggaccag
     2941 cgccgacagg tcggccagct ggtccttgct cacggtctga accggcgagt agctgcccat
     3001 gccgcctgtg ttcgggccgg tgtcaccttc accgatccgc ttgtagtcct gggccggggc
     3061 gagggctatt gcccgctcac cgtcgcagat cgcgagcagc gagagctcct cgccctcgag
     3121 gaattcctcg accacgacct cggtctggcc gaaacgctgc tcggtgaaga agacctcgat
     3181 cgcctcgcgg gcctcggctt cgtccatgca gatgagtacg cccttgccgg cggccaggcc
     3241 gtcggccttg agcaccaccg ggcagccggt ctcggcaacc ttggccaggg ccgagtcacg
     3301 gtcggtcacc accccgtagc cggcggtcgg caccccggct tcctccatcg cctccttggc
     3361 gtaggccttc gagccttcga gctgcgccgc ggcaccactc ggtccgaagg cggcgattcc
     3421 ggcctcggtc agggcgtcga ccaccccggc caccagcggg gcttccgtcc cgaccacaac
     3481 caggtt
//
LOCUS       DAOTIP010000128         3479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32003_length_3479_cov_3.184287, whole genome shotgun sequence.
ACCESSION   DAOTIP010000128 DAOTIP010000000
VERSION     DAOTIP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32003_length_3479_cov_3.184287"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="P5138_03440"
     CDS             complement(<1..587)
                     /locus_tag="P5138_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59659.1"
                     /translation="MRKGALKKKQRQLEELHLDRREALGELVLGMYVQNNWDDDLLAR
                     GSAEVAEVETELNELIAGNEPEGPSATEKDFPVTGEHRLPTGEHDYPATGEYTAEHAV
                     PVTGEHTAEHALPESAEHDYPATGEYTAEHALPESAEHTAEHAVPPPQPKLSRAEKKA
                     AKKEKKERERQEKEAARAAEEPATPVPAPAPSATAP"
     gene            complement(587..1612)
                     /locus_tag="P5138_03445"
     CDS             complement(587..1612)
                     /locus_tag="P5138_03445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV59660.1"
                     /translation="MRVLVVMNFGADPATPQRGRWVVDQVEALREGGLEVDLFSFRPG
                     KDQYLPATRKIRRILRQGDYDLVHAHYGLAGWCAQLAGADPLVVTFHGTDVRHRVVGR
                     MSRALTRKIALVAAASKALFEEEAGRPGLPLPEGRSAVLPCGADLSRFEPLNRRESRT
                     ELNLDPGEPFLFFPADPERPEKRFDRAQEVAERAGCTLRTGGGIDPDRMPLWVNAANA
                     VLVTSDYEGFGLACLEALACDVPVLSTPVGIAPHALRGLDRSLCREFDPDAWSDFASR
                     LVSEEDPRTDGRPRAEALSAQRMADRVALAYRDLLSEGPFDALSISANSGGARVSGRD
                     LGNDGVS"
     gene            1789..2037
                     /locus_tag="P5138_03450"
     CDS             1789..2037
                     /locus_tag="P5138_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59661.1"
                     /translation="MIYKAMGNAHALVVSRGDLAKRRVAERSGQGTVEYVGLILLVSL
                     LMLGMVAAMKGFSGRQGTELAELIVDKIQSAVDKIAFR"
     gene            complement(2034..2972)
                     /locus_tag="P5138_03455"
     CDS             complement(2034..2972)
                     /locus_tag="P5138_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59662.1"
                     /translation="MSMHRDEREWIWPDFGEQELIDRLLMPEPDFHNLWLNMMDQMPP
                     REYSDEVFETGTGYPWPRPDGSFILGEGEGRLLSELETAEREALLEEFTGPGSGRTPM
                     LAIGSNASPEGLWRKFGHFEDPADRTLLAVAGRIHDFDVAATAELALYGALPATIVPS
                     QGTKVSAMAVWLTDAQLTQLAWAEIPYWIGRLDTRFEFEPVVAEAGQEGFDRALVFVN
                     RFGAFGPHGHPLALGAIPAEDRTVPALSQVELLRLTAELTYGSAVTAEQLVRRAFENP
                     NETGAAVTGIMRSNSIPFESDRWTPYPGPGGRSPAG"
     gene            3007..>3479
                     /gene="rph"
                     /locus_tag="P5138_03460"
     CDS             3007..>3479
                     /gene="rph"
                     /locus_tag="P5138_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRV59663.1"
                     /translation="MSETVSRSGGRSAAELRPVKIEPGFVGSADGSALISCGGTRVIC
                     TASVQDRVPKWMEGRGRGWVTSEYAMLPASTGDRKQRDISKGKQDGRGVEIQRLIGRS
                     LRVGIDFDALGERSVYVDCDVLEADGGTRCASITGGWVALRLALGALLEAGEIDR"
BASE COUNT          559 a         1154 c         1154 g          612 t
ORIGIN      
        1 ggagcggtgg ccgaaggggc cggagccggt acgggggtgg ccggctcctc ggcggccctg
       61 gccgcctcct tctcctggcg ctcgcgttcc ttcttctcct tcttcgccgc cttcttctcg
      121 gcccggctca gcttcggctg cggcggcgga acggcatgct cggccgtgtg ttcggcggac
      181 tcgggcaggg catgctcggc cgtgtactcg ccggtcgccg ggtagtcgtg ttcggcggac
      241 tcgggcaggg catgctcggc tgtgtgctcc ccggtgaccg ggaccgcgtg ttccgccgtg
      301 tactcgccgg tggccgggta gtcgtgctcg ccggtcggca ggcggtgctc gccggtgacc
      361 gggaaatcct tctcggtggc cgaaggtccc tccggctcat tgccggcgat cagttcattc
      421 agctcggtct cgacctctgc cacctcggcc gatccgcggg ccagcaggtc gtcgtcccag
      481 ttgttctgga cgtacatccc gaggaccagc tccccgagcg cctcacggcg gtcgagatga
      541 agctcctcga gttgcctctg cttcttcttg agggcaccct tcctcatcag ctcaccccat
      601 cattacctaa gtcgcgaccg gaaacgcgcg cgcctccgga atttgcagaa atcgaaagtg
      661 cgtcaaacgg tccttcggaa agcagatccc ggtaggccag agccaccctg tcagccatcc
      721 gttgagccga gagcgcctcc gcccggggac gaccgtcggt ccgcggatct tcttccgaga
      781 ccagtcggga agcgaaatca ctccaggcat ccggatcgaa ctcccggcag aggctccggt
      841 ccagtccgcg caatgcgtgt ggcgcaatac cgaccggagt cgaaagaacc ggaacgtcgc
      901 aggcgagtgc ttcaaggcag gcgagtccga atccctcgta atccgaagtc accagcaccg
      961 cgttggcggc attgacccag agcggcatcc ggtcgggatc gatgccgccc ccggtccgca
     1021 atgtgcaacc tgcccgttcg gctacctcct gcgcgcggtc gaatctcttc tcgggcctct
     1081 ccgggtcggc cgggaagaag agaaacggtt ccccgggatc aaggttgagt tcggtccggg
     1141 actccctgcg gttcagcggc tcgaacctgg acagatcggc cccgcagggc agcaccgccg
     1201 aacgtccctc gggaaggggc agccccggcc ggccggcttc ctcctcgaag agagcctttg
     1261 acgctgccgc gaccagcgcg atcttccggg tgagtgcccg cgacatgcgg ccgacgaccc
     1321 ggtgccggac gtcggtcccg tgaaaggtca cgaccagcgg atcggccccg gccagctgtg
     1381 cgcaccatcc ggccaggcca tagtgcgcgt ggaccaggtc gtagtcgccc tgccgcagaa
     1441 tccggcggat cttccgggtg gcgggcagat actggtcctt gcccgggcgg aagctgaaca
     1501 ggtcgacttc gaggccaccc tcccggagcg cctcgacctg atcaacgacc catctcccgc
     1561 gttggggcgt ggccggatcg gccccgaagt tcataacgac cagaacccgc atccgggcag
     1621 gttatcccgc gcacccgctg ctttcaccaa cccgggcaag gatctttcgc cggacgtgcg
     1681 catcggtgaa ccccgcttcc gtcaattgtt gccttctcgt cttgttccgt cagatcttct
     1741 gccggacgat ccggaccaga acaacccact cgatggagga ggaaaacgat gatctacaag
     1801 gcgatgggaa acgcacatgc cctggtcgtg tcaagaggtg atctggccaa gcggcgggtg
     1861 gccgaaaggt cgggtcaggg cacggtcgaa tacgtcggcc tgatcctgct cgtttcgctg
     1921 ctcatgctcg gcatggtggc cgcgatgaag gggttctcgg gccgtcaggg caccgagctg
     1981 gccgagctga tcgtggacaa gatccagtcg gcggtcgaca agatcgcctt ccgctagccg
     2041 gcgggtgatc gaccacccgg tcccggataa ggggtccagc ggtcggattc gaaggggatc
     2101 gaattcgacc gcatgattcc ggtcaccgcc gcccctgtct cgttcgggtt ttcgaaggcc
     2161 cgcctcacca actgctcggc ggtcaccgcg gagccgtagg tcaactcggc ggtcaaccga
     2221 agcagctcga cctggcttag cgccggaacc gtgcgatctt ccgccggaat cgcccccagg
     2281 gcgagcggat ggccgtgcgg accgaaggcg ccaaagcggt tcacgaagac gagcgcgcga
     2341 tcgaacccct cctgtccggc ttcggccacc accggttcga actcgaagcg ggtgtcgagg
     2401 cgcccgatcc agtaggggat ctcggcccag gcgagctggg tcagctgggc gtcggtcagc
     2461 cagaccgcca tcgcactgac cttcgtgccc tggctgggaa cgatcgtggc cggaagggct
     2521 ccgtataggg ccagttccgc ggtcgccgcg acgtcgaaat cgtgaatccg gccggcgaca
     2581 gccagcaggg tccggtcggc cggatcctcg aagtggccga acttccgcca gagcccttcc
     2641 ggggatgcgt tcgaaccgat cgccagcatc ggtgtccggc cgcttccggg accggtgaat
     2701 tcttcaagca gagcttcccg ttctgccgtt tccagctcgg acaggaggcg accctcgcct
     2761 tcgccgagaa tgaaggagcc gtccggtctg ggccacgggt agccggtccc ggtttcgaag
     2821 acctcgtcgc tgtactcgcg gggaggcatc tggtccatca tgttcagcca gagattgtga
     2881 aagtccggct cgggcatgag cagccgatcg atcagctcct gttcgccgaa gtccggccag
     2941 atccactcgc gctcgtcccg gtgcatgctc accgcgacgg tagccggtct ggaagactca
     3001 gggcaaatga gcgaaaccgt ttcccgcagc ggtggccggt cggccgccga actccgtccc
     3061 gtcaagatcg aacccggctt cgtcggcagc gccgacgggt ccgcccttat ttcctgcggc
     3121 ggcacccggg tcatttgcac cgcttcggtg caggaccggg ttccgaagtg gatggaaggc
     3181 cgtgggcggg gctgggttac ctccgaatac gcgatgttgc ccgcctcgac cggtgaccgg
     3241 aagcagcgcg acatcagcaa gggcaagcag gacggccgcg gggtagagat ccagcgtctg
     3301 atcgggcgtt cgctgagggt cgggatcgac ttcgatgctc tcggtgagcg cagcgtctac
     3361 gtcgactgcg acgtgctcga agccgacggg ggaacccgtt gtgcctcgat tacgggtggc
     3421 tgggtggcgc tgcgtctggc gctcggggcc ctgctcgaag ccggcgagat cgatcggat
//
LOCUS       DAOTIP010000129         3469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32141_length_3469_cov_3.205038, whole genome shotgun sequence.
ACCESSION   DAOTIP010000129 DAOTIP010000000
VERSION     DAOTIP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32141_length_3469_cov_3.205038"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="P5138_03465"
     CDS             <1..1345
                     /locus_tag="P5138_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59664.1"
                     /translation="GEVKTYEVKATVDAWGDADPSADHLLDVQKVETQIDLNPGETKT
                     VQAVCPSGYFASDGSVRIDHVDQGTGDWTAPEVLESRASSLEAWQGTVHNSATGRAQA
                     KIFAVCIQKKTVADGTHQHDLIVSAPITVNNFTPAGKNEATLLCGPGQIAIQPGFESD
                     VPANLVYSEPEGNGWKFVLDSSQDGQATFSIRCMTRQVSVVDGHTHDLAFQHIVKEFT
                     IQPGEVNEAQLTCADGYKGIVADMDLDDGLVSLGNDPRPVTRAFKIYNPTDHALNARL
                     SLLCLGLRTAGEHVPPKVLQNTAYISTSSLEEVTTNNHSTATVVAEDTDNYEPVDPTE
                     PTKPTPNNPVAKTIVGKGVSYSASGVTFTLKCSGACGGTAKLSTMKKVKVKGKKFGKG
                     TVLAKKRYFIGKAGTKKVKLVPTRAGRTILKKGKARKAMLRISGGTRKVVKVRRR"
     gene            complement(1412..1750)
                     /locus_tag="P5138_03470"
     CDS             complement(1412..1750)
                     /locus_tag="P5138_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59665.1"
                     /translation="MSDQQNPATSSSNEKVPKGSYAAGRRVSLPEGAEQPIVVFINGV
                     AQSEGEDYVLRGEEILFTREIIKEEKSGKKKLVMLLGVVGFYNKDETIDVQFQKDGKT
                     ELAGDLPVSK"
     gene            complement(1747..2091)
                     /locus_tag="P5138_03475"
     CDS             complement(1747..2091)
                     /locus_tag="P5138_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59666.1"
                     /translation="MANWKLTIRHGSDVSRQSFDDLDDALGAARKAADEVMDEGPLDS
                     VSAIRDYEPAQIVNARIEISGKGFFSPPTAGIDIQGDLSVIPFAGGVKRQTLEGSTIP
                     QAMKSLKMFLSS"
     gene            complement(2104..>3469)
                     /locus_tag="P5138_03480"
     CDS             complement(2104..>3469)
                     /locus_tag="P5138_03480"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRV59667.1"
                     /translation="EAPAEDDGGGVATGTDTLSVTDNRTGRTYELPIEDDTIKALDLR
                     QIKVHEDDFGMMAFDPAFTNTASCRSSITYIDGEAGILEHRGIPIEQLCEHSTFLEVA
                     YLLVFGELPTRPQLEAWVHDVTHHTFVHEDIKKFLSGFRYDAHPMGMLLASTGALSTF
                     YPDSNQIQDPVERYLATIRLIAKMPTLAAFSYRHNMGLPYVYPDNDLTYPENFLSMMF
                     KMTEAKYEPDPRLAKAIDVLFILHADHEQNCSTSAVRGVGSSDVDPYSAVAAGIAALY
                     GRLHGGANEAVLQMLRRIESVDNIPSFLEAVKNREEKLMGFGHRVYKNYDPRARIIKS
                     HADEVFEATEYNPLVELATELEKQALDDEFFTSRKLYPNVDFYSGIIYEALQIPPGMF
                     TVIFAIARTAGWISQWLEMTEDPDKKIARPRQIYTGAREADYVPIGERTGEDRISGPA
                     TRPS"
BASE COUNT          635 a         1067 c         1121 g          646 t
ORIGIN      
        1 gggtgaagtc aagacctatg aggtcaaggc gaccgtcgat gcctggggag acgccgatcc
       61 ttcagccgat cacctgctgg acgtccagaa ggtggaaacc cagatcgacc tgaacccggg
      121 cgagacgaag acggtccagg ctgtctgccc gtccggctac ttcgcctccg acggttcggt
      181 gcgtattgat catgtcgatc agggaaccgg tgactggacc gcgccagagg tcctcgagtc
      241 gagggccagc tcgcttgaag cgtggcaggg caccgtccac aactccgcga ctggtcgcgc
      301 ccaggcgaag atcttcgcgg tctgcatcca gaagaagacg gtcgccgacg gtacccacca
      361 gcatgatctg atcgtgtcgg cgccgatcac ggtgaacaac ttcactccgg ccggcaagaa
      421 cgaggcgacg ctcctgtgcg gtcccggcca gatcgcgatc cagcccggct tcgagtcgga
      481 cgttcccgcg aacctcgtct acagcgagcc ggagggcaac ggctggaagt tcgtgctcga
      541 ctcgagccag gacggtcagg ccaccttctc gatccgctgc atgacccgcc aggtcagcgt
      601 cgtcgacggt cacacccacg acctcgcctt ccagcacatc gtcaaggagt tcaccatcca
      661 gccgggtgag gtcaacgagg cgcagctgac ctgcgctgac ggctacaagg gcatcgtcgc
      721 cgacatggac ctcgatgacg gcctggtctc gctgggcaac gatccgcggc cggtgacccg
      781 tgccttcaag atctacaacc cgaccgacca tgctctgaac gccaggctct cgctgctctg
      841 cctcggtctc aggaccgccg gtgagcacgt gccgccgaag gtgctgcaga acaccgccta
      901 catctcgacc agctcgctcg aagaggtgac caccaacaac cactcgaccg cgaccgtggt
      961 tgccgaggac accgacaact acgagccggt cgacccgacc gagccgacca agccgacccc
     1021 gaacaatccg gtcgcgaaga cgatcgtcgg caagggtgtc agctactcgg cttcgggggt
     1081 caccttcacc ctcaagtgct cgggtgcctg tggcggaacg gcgaagctct cgaccatgaa
     1141 gaaggtgaag gtcaagggca agaagttcgg caagggaacc gtgctcgcga agaagcggta
     1201 cttcatcggc aaggccggga cgaagaaggt caagctcgta ccgacccggg ccggacgcac
     1261 cattttgaag aagggcaagg cccgcaaggc catgctccgc atatctggag ggaccagaaa
     1321 ggtagtgaag gtcaggcgcc gctaggaaag cgaccttctg ccagggaact ggagcctcgg
     1381 tcgcgagacc ggggctcttt ctcttgcagg gttacttcga gaccgggagg tcgccggcaa
     1441 gttcggtctt gccgtccttc tggaactgga cgtcgatcgt ctcgtccttg ttgtagaagc
     1501 cgaccacacc gagcagcatg accagcttct tcttcccgga cttctcttcc ttgatgatct
     1561 ccctcgtgaa gaggatctct tcccctcgca gcacgtagtc ctcgccttcg gactgggcca
     1621 ccccgttgat gaagaccacg atcggctgct cggcaccctc cggcagcgag acacgccgcc
     1681 cggcggcata ggagcctttg gggaccttct cattcgatga actggtggct gggttctgct
     1741 ggtcgctcat gagctgagga acattttgag ggatttcatc gcctgcggga tggtcgagcc
     1801 ttcgagcgtc tgccttttca ccccgccggc gaagggaatg accgagagat cgccctggat
     1861 gtcgatcccc gcggtcggcg gagaaaagaa ccccttcccg ctgatctcga tgcgggcatt
     1921 gacgatctgg gcgggctcgt agtcacgaat cgcgctgacc gaatcgagtg gtccctcgtc
     1981 catcacctcg tcggccgcct tgcgggccgc gcccagggcg tcatcgaggt catcgaagct
     2041 ctggcggctc acatcggagc cgtggcggat ggtcagcttc cagttggcca cggcacccga
     2101 ggttcaggaa ggcctggtgg cgggaccgga gatccggtcc tcgccggtgc gctcgccgat
     2161 cggcacgtag tccgcctcgc gggcgccggt gtagatctga cggggccggg cgatcttctt
     2221 gtccgggtcc tcggtcatct ccagccactg ggatatccag ccggcggtgc gggcgatcgc
     2281 gaagatcacg gtgaacattc cgggcgggat ctgcagcgcc tcgtaaatga tccccgagta
     2341 gaagtccacg ttcgggtaga gcttgcggga ggtgaagaac tcgtcgtcga gtgcctgttt
     2401 ctcgagttcc gtcgccaact cgaccagcgg gttgtattcg gttgcctcga agacctcgtc
     2461 ggcgtggctc ttgatgatcc tggcccgcgg gtcataattc ttgtagaccc ggtggccgaa
     2521 gcccatcagc ttctcttcgc ggttcttgac cgcttcgagg aaggacggaa tgttgtcgac
     2581 cgattcgatg cggcgcagca tctggagcac cgcctcgttg gcgccgccat ggagccggcc
     2641 gtagagcgcg gcgatgccgg cggcgaccgc cgagtacgga tcgacgtcgg aggaaccgac
     2701 gcccctgacc gcgctggtcg aacagttctg ctcgtggtcg gcgtgaagga tgaagagtac
     2761 gtcgatcgcc ttggccagcc tggggtccgg ctcgtacttc gcctcggtca tcttgaacat
     2821 catcgagaga aagttctcgg gataggtcag gtcattgtcc ggatagacat agggcagacc
     2881 catgttgtgt cgatacgaga aggccgcgag ggtcggcatc ttggcaatca gcctgatggt
     2941 cgccagatac cgctcgaccg gatcctgaat ctggttcgaa tccggataga aggtggagag
     3001 cgccccggtc gaggccagca gcatccccat cgggtgggcg tcgtagcgga agccggaaag
     3061 gaacttcttg atgtcttcgt ggacgaaggt gtggtgggta acgtcatgaa cccaggcctc
     3121 gagctgcggc cgggtcggca gctcgccgaa taccagcagg taggccactt cgaggaaggt
     3181 cgagtgctcg cagagctgct cgatcgggat tccgcgatgt tccaggattc ccgcttcccc
     3241 gtcgatgtag gtgatcgacg agcggcagga cgcggtgttg gtgaaggccg ggtcgaaggc
     3301 catcatcccg aagtcgtctt cgtggacctt gatctggcgc agatcgaggg ccttgatcgt
     3361 gtcgtcctcg atcggcagct cgtaggtgcg ccccgtccgg ttgtcggtga cgctgagggt
     3421 gtcggtaccg gtggctacgc cgcctccgtc atcttcggcg ggcgcttcg
//
LOCUS       DAOTIP010000130         3459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32266_length_3459_cov_2.982961, whole genome shotgun sequence.
ACCESSION   DAOTIP010000130 DAOTIP010000000
VERSION     DAOTIP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32266_length_3459_cov_2.982961"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5138_03485"
     CDS             <1..230
                     /locus_tag="P5138_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HRV59668.1"
                     /translation="PDPERGSIVKAVVVLREGHETHDGGETESLVSELQEHCRRETAP
                     YKYPRVVEFIDELPKTTSGKIRRAELRAREN"
     gene            complement(374..1462)
                     /locus_tag="P5138_03490"
     CDS             complement(374..1462)
                     /locus_tag="P5138_03490"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRV59669.1"
                     /translation="MTAIQKDPDNPIAHLSREDIEQIGRELDAIRQEVLDTRGEEDAA
                     YIRGLIDTQRKLELGSRGVLLFSLFPPAWLAGTAGLTVAKILENMEIGHNVMHGQWDW
                     MRDPKIHSTTWEWDNASPADLWKHSHNEIHHTYTNVIGKDHDLGYGIMRVDEDQRWVP
                     FYLGQPAWNLINAFLFEYSIAAYDLQLKKNGKDRKDNPEFQAKAKQVIKKIRAQMTKD
                     YVVHPVLSGPSAPATLAANLTANLVRNLWTHSVIMCGHFPEGVATFEKTSIDGETRDD
                     WYVRQMLGAANIKGSPAMHVMTGNLSFQIEHHLFPDLPSNRYQEISPKVKEIFDRYDL
                     PYAEGSLPRQVASAWKKVIRLSLPDDTI"
     gene            complement(1470..2561)
                     /locus_tag="P5138_03495"
     CDS             complement(1470..2561)
                     /locus_tag="P5138_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HRV59670.1"
                     /translation="MPSLLSPRRSVSGIRTGVTALAERVATPLVPSDYIDLVSPLASG
                     AELRGRIVGVLPETADAATLVIKPGRAWEGHRPGQYVRVGVDIDGVRHWRAYSLTSSP
                     DRPDRNITISVKAIEDGLVSNHLVHRVQAGTVIQLDQATGDFTLGERSERKQLFITAG
                     SGITPVMGMLRGHPEKLRGSVLIHSAPTGRDVIFGRELRGLADDGRIELIEIHTDRVG
                     MLAPANLEAMVPDLAEREIRACGPTAMLDSIEEHLETLGLADRLHTERFRPVVVVTGE
                     GGVIDFTESQISAEGDGATPILDVGEGAGVLMPSGCRMGICFGCVSRLREGAVRDLRT
                     GEVTTASAGDEIQIQTCVSAAAGTCQIEL"
     gene            2668..>3459
                     /locus_tag="P5138_03500"
     CDS             2668..>3459
                     /locus_tag="P5138_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59671.1"
                     /translation="MSTESLFPPETVIASLREVLPDVATASAAAVIAEVPEYGTLRGP
                     EGDTLDNQVELALRGFLVIASESRSADAVVPFQPALDASFALGRGEFRSGRTTDVLLS
                     AYRIGARAAWREWSRVAIDAGISIELFSRFAEMIFAYIDQLSASSVAGYSHELESSQR
                     SRSRQLEELARRILAGGPVDALRDEAERVQWDPPEAVSAVIVATGRAQGIRNLIDARS
                     LIVPDDLPGLAGEDRLLVLAPTSRPDEREHLKGALEGRGVVIGPAR"
BASE COUNT          580 a         1113 c         1101 g          665 t
ORIGIN      
        1 cacccgaccc ggagagaggt tccatcgtca aggccgtggt cgtgctccgg gaaggccacg
       61 agacgcatga cggcggggaa accgaaagtc tcgtgtcgga acttcaggag cattgccgcc
      121 gcgaaaccgc tccgtacaag tacccccggg tggtcgagtt catcgacgaa ctgccgaaaa
      181 cgacctccgg gaagatccgc cgggccgagc tccgggcccg cgaaaactga ttgctcgcga
      241 gagctggagc ggcggagagc cgcagtgatg gcggaaccgg tcaggccgcg tgggcttcca
      301 accgtgccgg gcgaatctcg tgaacgtccg catccttctt tctcctgccg aacccgaaca
      361 gcgatttcgc ctttcagatc gtgtcgtcgg ggagtgaaag acgaatgacc ttcttccagg
      421 ctgacgccac ctgcctgggc aggctgccct ccgcgtaggg gaggtcgtag cggtcgaata
      481 tctccttcac ctttggcgag atctcctgat accggttgct cggcaggtcc gggaacaggt
      541 ggtgctcgat ctggaacgaa aggtttccgg tcatcacatg cattgccggc gagcccttga
      601 tgttggccgc tccgagcatc tgcctcacat accagtcgtc gcgggtctcg ccgtcaatcg
      661 acgttttctc gaaagtggca acgccttcgg ggaaatggcc gcacatgatc accgagtggg
      721 tccagaggtt ccggacgagg ttcgcggtca ggttggctgc gagagtcgcc ggcgccgacg
      781 ggcccgaaag gaccgggtga accacataat ccttggtcat ctgggcccgg atcttcttga
      841 tgacctgctt tgccttggcc tggaattccg gattgtcctt ccggtctttc ccgttcttct
      901 tcagttgaag gtcgtaggcc gcgatgctgt actcgaagag gaacgcgttg atcaggttcc
      961 aggcgggctg accgagatag aacggcaccc agcgctggtc ctcgtccacc cgcatgatcc
     1021 cgtaacccag gtcgtgatcc ttgccgatca cgttggtgta ggtgtggtgg atctcattgt
     1081 gggagtgctt ccacaggtcg gcgggggaag cgttgtccca ttcccaggtg gtcgaatgga
     1141 tcttcgggtc ccgcatccag tcccactggc catgcatgac gttgtggccg atctccatgt
     1201 tctcgagaat cttggccacg gtgagtccgg ccgtgccggc cagccaggcc ggcggaaaca
     1261 gcgaaaagag caagacgcca cggctgccca actccagttt gcgctgggtg tcgatcagcc
     1321 cgcgtatgta ggcggcatcc tcttctccgc gcgtgtcgag cacctcctgg cggatcgcgt
     1381 cgagttcgcg gccgatctgc tcgatgtcct cgcgcgagag gtgggcgatc gggttgtctg
     1441 gatctttctg gatagcggtc attgcttcct cacaattcga tttggcaggt gccggcagcc
     1501 gccgacacac aggtctggat ctgaatttcg tcaccggccg aggcggtggt cacctcgccg
     1561 gtccgcaggt cccggacggc tccctcgcgc agccgggaaa cacagccgaa gcagatgccc
     1621 atccggcaac cggagggcat cagtaccccg gccccctcgc cgacgtccag gatcggggtc
     1681 gctccgtctc cctcggcgct gatctgcgac tcggtgaagt caatgacccc gccctccccc
     1741 gtgaccacga ccacagggcg aaagcgttcg gtgtggagtc ggtcggcaag gcccagggtt
     1801 tcgaggtgct cctcgatcga gtcaagcatc gcggtgggcc cgcaggccct gatttcccgc
     1861 tctgcgaggt cggggaccat cgcttcgagg ttggccgggg cgagcatccc gacccggtcg
     1921 gtgtggatct cgatcagttc gatgcgaccg tcgtccgcga gaccacgcag ttcccgtccg
     1981 aagatcacgt cccgcccggt cggggccgag tgaatgagta cggaacccct gagcttctcg
     2041 gggtggccac ggagcatccc catcacgggg gttatgccgc tgccggcggt gatgaaaagc
     2101 tgctttcgct ccgaccgttc cccgagggtg aagtcaccgg tcgcctgatc gagctggatg
     2161 accgttcccg cctggacccg gtggaccagg tggttgctga ccaggccgtc ctcgatcgcc
     2221 ttgacgctga tcgtgatgtt ccggtcgggc cggtccggcg acgaggtcag tgaataggcc
     2281 cgccagtgcc tcactccgtc gatgtcgacc ccgacgcgaa cgtactggcc cggtcgatgg
     2341 ccttcccagg ccctgcccgg cttgatcacc agcgtcgccg cgtcggcggt ttccggcagc
     2401 acgccgacga tccgtcccct cagttccgcg cccgaagcga gcggcgaaac aaggtcgatg
     2461 tagtccgacg gaacgagcgg tgtcgccacc ctttccgcca gggcggtcac cccggtacgg
     2521 atcccggaga cggagcggcg cggtgaaaga agcgacggca tacgagaagg gtggactatc
     2581 ccagctgcat tatcttgcct ctcttggttg aactatgttg cccgttttta ttgattccga
     2641 acaagtaagg cgctatcttc ggtcaccatg agcaccgaat ctctgttccc gccggaaacg
     2701 gtcatcgcga gcctgcgcga ggttctcccc gatgtcgcga cagcctcggc cgccgcggtc
     2761 atcgccgagg tgcccgaata cggcaccctg cgcggccctg aaggagacac tctcgacaat
     2821 caggtggaac tggccctccg cggcttcctg gtaatcgcat ccgagtcgag atccgccgac
     2881 gcggtagtcc ctttccagcc cgcactcgac gcatccttcg ccctcggccg gggcgagttc
     2941 cgcagtggcc ggacgaccga cgttctcctc tccgcgtacc ggatcggcgc cagggcggcg
     3001 tggcgggaat ggtcacgggt cgcgatcgac gcgggcatct cgatcgaact cttctcccgc
     3061 ttcgccgaga tgatcttcgc ctatatcgac caactgtctg cctcgagcgt cgccggctac
     3121 tcccacgaac tcgagtcgag ccagaggtcc agatcgcgtc agctcgaaga gctggcccgc
     3181 cggatcctcg cgggcggtcc ggtcgatgcc ctccgggatg aggcggaaag agtccagtgg
     3241 gatccacccg aagccgtttc ggccgtgatc gtcgcgaccg gaagagcgca gggcattcgc
     3301 aacctgattg atgcccgttc cctgatcgtc cctgatgacc tgcctggcct ggctggtgaa
     3361 gatcgcctgc tcgttctcgc acccacttca aggcccgacg agagggagca tctgaagggt
     3421 gccctggagg ggcgcggtgt ggtcatcgga cctgcgcgc
//
LOCUS       DAOTIP010000131         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32629_length_3431_cov_3.711493, whole genome shotgun sequence.
ACCESSION   DAOTIP010000131 DAOTIP010000000
VERSION     DAOTIP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32629_length_3431_cov_3.711493"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="P5138_03505"
     CDS             complement(<1..1351)
                     /locus_tag="P5138_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRV59672.1"
                     /translation="MAPRKTEHPSGSTSRQDGRANGASGEAGKDDAGDKAVPDSVLRP
                     PGGRSRSREARGKASGESKRRIGLPKLGRRRKKEDAAAKAGTPTASSPAAAGNGNGQP
                     LSIPPAPGAGGDGQKNGNGNGNGNGNGNGLPPGVLTLPVPPPKPPKPKIKKLRILFIV
                     LGLGVLGFCSAFFGMVMAVSQDLPAIENYAQFRTAKNSVLVDSQGQYIGTLSNNENQM
                     LLDSDQISPNMKNAVVSIEDSRFYEHRGIDFQGLGRALVQDIISRSAKQGASTITQQL
                     VKQALEAQNDRSPLQKVREMSIAYHIEQQWTKDKILTEYLNTVYFGNGAYGIEAAART
                     YFGYAHPSCGTDEEPCAALLTPAEAAMLAGIIQNPYGYDPKNDPDATLLRRNTVLDKM
                     LDQGYITQEQHDEATKEALPAGDDIDPPSLDSKAPYFTSWMRQQLVDRRLRARAYAYE
                     "
     gene            1469..2668
                     /gene="tyrS"
                     /locus_tag="P5138_03510"
     CDS             1469..2668
                     /gene="tyrS"
                     /locus_tag="P5138_03510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRV59673.1"
                     /translation="MSNVTEEPREPGPEAVARAAALTKTAVEALPAGRLAGQLDLGRP
                     LRIKLGIDPTAPDIHLGHTVVLNKLREFQEAGHQVVLIIGDYTARVGDPSGRSDQRPM
                     LDPEAIDANAETFMEQAFKILDREKTEVRYNSEWLKMESDQLFSLMARPTVARLLERD
                     DFQKRMAAEEPISLLELVYPLLQGYDSVAIDADVEIGGTDQKFNLLFGRDMQSSFGKE
                     LQSVMTLPILPGIDGVKKMSKSTGNYIGVTDPPEEMFGKVMRVPDANLKEYWQLLLST
                     DPPAGAGPNEAKRELGRKLVERFHGADAVPGAEEHFNRLFVRHEAPEDVEDFKFGEDP
                     AHLPALVAGAFGVSRSEARRAIGQGGVKLDGKTLPAEPLDYPAGDLDGRVLQLGKRRF
                     RRLVNGA"
     gene            3091..>3431
                     /locus_tag="P5138_03515"
     rRNA            3091..>3431
                     /locus_tag="P5138_03515"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          601 a         1042 c         1133 g          655 t
ORIGIN      
        1 attcgtacgc gtacgcccgc gcgcgaagcc gccggtcgac cagctgctgg cgcatccagg
       61 aggtgaagta aggagccttc gaatcgaggc tgggcgggtc gatgtcatct ccggccggca
      121 gcgcttcctt ggttgcctcg tcgtgctgtt cctgggtgat gtaaccctgg tccagcatct
      181 tgtcgagcac cgtgttgcgt ctcagcaggg tggcatccgg atcgttcttc gggtcgtatc
      241 cgtaggggtt ctggatgatg ccggcaagca tcgcggcctc ggccggggtc agcagggcgg
      301 cgcagggttc ctcgtccgtg ccgcatgaag ggtgcgcgta tccgaagtag gtgcgggcgg
      361 cggcttcgat gccgtaggcg ccgttgccga aatagaccgt gttcaggtac tcggtgagga
      421 tcttgtcctt ggtccactgc tgctcgatgt ggtacgcgat cgacatctcg cggaccttct
      481 gcagcggcga gcggtcattc tgggcttcga gcgcctgttt gaccagctgc tgggtaatgg
      541 tcgaggcgcc ctgtttggcg ctgcgggaaa tgatgtcctg cacgagggcg cggccgagac
      601 cctggaagtc gatgccgcgg tgctcgtaga aacgcgagtc ctcgatcgag accacggcgt
      661 tcttcatgtt gggcgaaatc tggtccgagt cgagcagcat ctggttctcg ttgttggaga
      721 gcgtgccgat gtactgaccc tgactgtcga ccaggaccga gttctttgcg gtgcggaact
      781 gggcgtagtt ctcgatcgcc gggaggtcct gggagacggc catcaccatg ccgaaaaagg
      841 ccgagcagaa gccgagcacg ccgagcccga gcacgatgaa gaggatccgg agcttcttga
      901 tcttcggctt cggcggcttg ggcggaggca ccggcagggt gagcacaccc ggaggcaggc
      961 cgttcccgtt cccgttcccg ttgccgttcc cattgccgtt cttctggccg tcgcccccgg
     1021 ctcccggggc gggcggtatc gagagcggct ggccgttgcc gttgccggct gcggccgggc
     1081 tgctcgcggt cggggtgccc gccttcgccg cggcatcttc cttcttccgg cggcgtccga
     1141 gcttcggcag gccgatcctg cgtttggact cgccactcgc cttgccgcgg gcttcccggc
     1201 tgcgggatct gccgcccggc ggcctcagga cgctgtcggg cacggccttg tcgccggcat
     1261 cgtccttgcc ggcttcacct gacgcgccat tggcgcgccc gtcttgccgg gaggttgagc
     1321 ccgacgggtg ctctgtcttt cggggggcca tcagatggcg aaagtgtctg gcgcttcagc
     1381 gaaggttgtc ctggaatgcg ccgtggaact gcaaacaaca atgcggacac gaccaagtag
     1441 cgcttgtcag tctagcattg actctcccgt gtccaacgtc actgaagaac cccgtgagcc
     1501 ggggccggaa gcggtcgccc gggcagctgc cctgaccaaa accgccgtcg aggccctgcc
     1561 ggccgggcgc ctggccggcc agctcgacct cggccggccg ctccggatca aactgggcat
     1621 cgaccccacc gctcccgata tccatctcgg gcacacggtg gtgctgaaca agcttcggga
     1681 gttccaggag gccggacacc aggtggtcct gatcatcggt gactacacgg cccgggtcgg
     1741 tgacccgagc ggcaggtcag accagcggcc catgctcgac cccgaggcga tcgacgcaaa
     1801 cgccgagacc ttcatggagc aggccttcaa gatcctcgac cgggagaaga ccgaggtccg
     1861 ctacaacagc gaatggctga agatggagtc ggaccagctc ttctcgctga tggcgaggcc
     1921 gaccgtggcc cgactgctgg agcgcgacga cttccagaag cggatggcgg ccgaagagcc
     1981 gatctcgctg ctcgaactgg tctatccgct gctccagggc tatgactcgg tcgcgatcga
     2041 cgccgacgtc gagatcggcg gcacagacca gaagttcaac ctcctcttcg gccgcgacat
     2101 gcagtcctcc tttggcaagg aactccagtc ggtgatgacc ctgccgatcc tgcccggtat
     2161 agacggtgtg aagaagatgt ccaagtccac cggcaactac atcggagtca ccgatccgcc
     2221 ggaggagatg ttcggcaagg tgatgcgggt tccggacgca aacctcaagg agtactggca
     2281 actcctgtta agtaccgacc cgccggccgg agccggaccg aacgaggcca agcgtgaact
     2341 gggcaggaag ctggtcgaac ggttccacgg agccgatgcg gttcccgggg cggaagagca
     2401 cttcaaccgg ctcttcgtgc ggcatgaggc tcccgaggat gtcgaggact tcaagttcgg
     2461 cgaggatccg gcccacctgc cggcgctggt cgcgggagcg ttcggtgtaa gcaggagtga
     2521 agcccggcgg gcaatcgggc agggcggggt gaagctggac gggaaaaccc tcccggcaga
     2581 gcctcttgat tacccggccg gggacctgga cggccgcgtc cttcagctcg ggaaacgccg
     2641 ttttcggcgc ctcgtcaatg gcgcctgatc ccgctctggc aagggatttg gcttcccaga
     2701 gccaaaaccc aaatcattct ccgatcccca gcgaaatggc gcgacattcc gccggggaat
     2761 ctgtatactc cttcgtccgc tcagtcgagc aggttgcccg gctgaagtgc tctgtgcggc
     2821 gaaacccgcc gcccggcgca agcggtcttt gaaaactgag cagcgtgcat tactccggac
     2881 ggttcgccgt ctggggataa tgtttgagcc cgattcgttc gaagctttcc gcttcggacg
     2941 atcattaatt atcccgtcat atccgttgtc gtatttgtgt actgacaaca tcccgatatg
     3001 acacagctct acgttgagta aatagtttga ggaacccggt cttcggaccg gttgccggct
     3061 tcggccggca tttgatacct cgcaacattt ttcacggaga gtttgatcct ggctcaggac
     3121 gaacgctggc ggtgtgctta acacatgcaa gtggtgcgac gaaccaggct tcggcctggg
     3181 gcaaagccgc gaacgggtga gtaacacgtg ggtaatctgc cccgatgacc gggacaaccc
     3241 gtcgaaagac gggctaatac cggatgtggt gcccagacac aagtctggtc actaaaggta
     3301 gcttcggctt ccgcattggg aggggcccgc ggcccattag ctagttggtg gggtcaaggc
     3361 ctaccaaggc aacgatgggt agctggtctg agaggacgat cagccacact gggactgaga
     3421 cacggcccag a
//
LOCUS       DAOTIP010000132         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32634_length_3431_cov_3.155806, whole genome shotgun sequence.
ACCESSION   DAOTIP010000132 DAOTIP010000000
VERSION     DAOTIP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32634_length_3431_cov_3.155806"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /gene="arsM"
                     /locus_tag="P5138_03520"
     CDS             complement(<1..728)
                     /gene="arsM"
                     /locus_tag="P5138_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRV59674.1"
                     /translation="MAELEQVNDCCAPDTRASCCETSAKEACCGEGPGCECSAGTERT
                     ESELREIVRERYAAAAVDAGSRDASCCANDAVITEEQRAVFGSSLYEEGERSELPEAA
                     QLASLGCGNPTAVADLEEGQTVLDLGSGGGIDVLLSARRVGPSGKAFGLDMTDEMLEL
                     ARSNQKEAGVENVEFLKGEIEAIPLPDSSVDVVISNCVINLSGDKARVLREAARVLRP
                     GGRFAVTDVIADPDMAFNVTFGEI"
     gene            complement(716..1069)
                     /locus_tag="P5138_03525"
     CDS             complement(716..1069)
                     /locus_tag="P5138_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV59675.1"
                     /translation="MSPELTLTPKQKSPNGEPCCEPVVYPDVERAEAEQMARIAKALG
                     DPIRFTLVDVLRKHAGKVCVCELVPLFDVSQSTLSHHLKKLRDAGIVDSERQGLWAYY
                     YVNPEELEELSAWLS"
     gene            1155..2183
                     /locus_tag="P5138_03530"
     CDS             1155..2183
                     /locus_tag="P5138_03530"
                     /inference="COORDINATES: protein motif:HMM:NF033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HRV59676.1"
                     /translation="MTRTLRVGINGFGRMGRLAIRAGWDSPSIEFVHINEIAGGPESA
                     AHLLAFDSVQGRWREDEVESTASSILVGGSEIGYSSETEPDAVDWGELGVDLVIEATG
                     EFRKPGLLAPYFDRGVASVLVAAPVKEDALNIVFGVNDSEYEPDRDRIVTAASCTTNC
                     LAPVVKVVHEGIGISHGSITTLHDLTNTQVVVDAHHKDLRRARAAGTSLIPTSTGSAT
                     AIGMIFPDLQGRLDGLAVRVPLLNSSITDCCFEVDRPTTVDEVNGMLSVASEGDLSGI
                     LGFESRPLVSVDFKGDPRSGIVDGTSTRVINETQVKILAFYDNEWGYANRMIDIAEMI
                     GGKLLSED"
     gene            2187..>3431
                     /gene="arsJ"
                     /locus_tag="P5138_03535"
     CDS             2187..>3431
                     /gene="arsJ"
                     /locus_tag="P5138_03535"
                     /inference="COORDINATES: protein motif:HMM:NF033734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HRV59677.1"
                     /translation="MTGTARREGDLRNYILVTAAYWADTLADGASRILVLFFFYQLGY
                     SALEVATFSLFYEFFGIVTNLVGGWIASRMGLKATLFSGLGIQIVALGMLGFAPDAWL
                     VVPYVMASQALSGIAKDLTKMSSKSAVKLVVPEDSESSLFKWVAILTGSKNALKGVGF
                     FLGGLLLTVIGFQSAMLVLLGIVATALVTTSTLMEGSLGYSDMGAKFRHMFSHTREIN
                     LLAAARLFLFASRDVWFVVALPVYLRTVLDWSFWAAGGFLAIWVIGYGAVQASAPGLF
                     ARRAKQTGDTPTGRTAARLAFLLAAFPAAIAASLAANLDPTFVLVVGLILFGIVFALN
                     SAVHSYLVLAYAEDETVAKNVGFYYMANAGGRLIGTILSGAVYQWLGLEGCLWFSVGL
                     VLIAATISLWLPSDQKRVPVEAM"
BASE COUNT          584 a         1101 c         1075 g          671 t
ORIGIN      
        1 atgatctcgc cgaatgtgac attgaaggcc atgtcgggat cggcgatcac gtcggttacg
       61 gcgaatcgac cccctggtcg caggacgcga gcggcctccc tgaggaccct ggccttgtcg
      121 ccggagaggt tgatcacgca gtttgagatc accacgtcaa ccgaggagtc cgggagaggt
      181 atcgcttcga tctctccctt caggaattcg acgttctcga cacccgcctc tttctgattg
      241 ctgcgagcga gctccagcat ctcgtcggtc atgtcgagac cgaaggcctt tccactcgga
      301 ccaacccgcc tggccgagag cagaacgtcg atgccgccac cggatccgag atcaagaacg
      361 gtctgtccct cctcgagatc ggcgacagcg gtcgggttgc cgcagccgag agaagcgagc
      421 tgcgcggctt cgggcagttc gcttcgttcg ccctcttcgt agaggctgga accgaacacg
      481 gcccgctgct cttcggttat gaccgcgtca tttgcgcagc aggacgcatc ccgggagccg
      541 gcgtcgaccg cggcagccgc gtacctctcc cggacgattt ccctgagttc cgattcggtg
      601 cgttcagttc ctgctgagca ttcacagcct ggaccctcgc cgcaacaagc ttccttggcg
      661 ctggtctcgc agcatgaagc tcgcgtatcc ggggcgcaac agtcgttgac ttgttctagc
      721 tcagccatgc gctcagttcc tcgagctctt ccgggttcac gtagtaataa gcccagagac
      781 cctggcgttc ggaatccacg atgccggcgt ccctcagctt cttcagatgg tgggagagag
      841 tggactggct gacgtcgaaa agggggacga gttcacaaac gcagaccttg ccggcgtgct
      901 tgcggagaac gtcgacgagg gtgaagcgga tcggatcccc cagggccttt gcgatcctgg
      961 ccatctgctc ggcctcggcc cgttccacat ccgggtagac gaccggttcg caacagggct
     1021 cgccgttggg actcttctgt ttgggggtga gggtcagttc ggggctcatc gaaattcatc
     1081 aaatcagttc aattcacgtt tgtcaataga tacgatgccc ttcgatagag caaacgaggc
     1141 ctggaggcca ccggatgaca cgcacgctga gagtcgggat caatggcttc gggagaatgg
     1201 gccgcctggc aatccgggcc ggctgggatt caccctcgat cgaattcgtc catatcaacg
     1261 agatcgccgg cgggccggaa tcggccgccc acctgctcgc cttcgattcg gtgcagggac
     1321 gctggcggga agacgaagtc gaatcgaccg cttcctcgat cctcgtcgga ggatcggaga
     1381 tcggctacag ctcggaaacg gagcccgacg ccgtggactg gggagagctc ggcgtcgatc
     1441 tggtgatcga ggcgaccggc gagttccgca agccgggact gctcgccccc tacttcgacc
     1501 ggggtgtggc cagcgtcctg gtcgcggccc ctgtcaagga ggatgcgctc aacatcgtgt
     1561 tcggggtgaa cgattccgaa tacgaaccgg accgggaccg gatcgtgacc gccgcttcct
     1621 gcaccaccaa ctgcctggcc ccggtggtca aagtggttca cgaaggcata ggcatcagcc
     1681 acggctcgat caccacgctg catgacctga ccaacaccca ggtcgtggtc gacgcccacc
     1741 acaaggacct gcgtcgggca agggccgccg ggacctcgct cattcccacc tccaccggat
     1801 cggcgaccgc gatcggcatg atcttcccgg accttcaggg ccggctggac gggctggcag
     1861 tccgggtgcc cctgctcaac tcttcgatca ccgactgctg tttcgaggtc gaccggccga
     1921 ccacggttga cgaggtgaac ggcatgctct cggtcgcctc cgaaggggat ctgagcggga
     1981 tcctcggttt cgagtcccgg ccgctggtct cggtcgactt caagggcgac ccgaggtccg
     2041 ggatcgtgga cgggacatcg accagggtaa tcaatgaaac ccaggtgaaa atcctcgcct
     2101 tctacgacaa cgagtgggga tacgcgaacc gaatgatcga catcgcggag atgatcggcg
     2161 ggaaactgct ctcagaggac tgagcgatga ccgggaccgc gagaagagag ggtgatctca
     2221 ggaactacat cctggtcacc gccgcctact gggcggacac cctggccgac ggggccagcc
     2281 gcatcctggt cctctttttc ttctaccagc tcggctacag cgccctcgag gtcgcgacct
     2341 tctccctgtt ctacgagttc ttcgggatcg tcaccaacct ggtcggcgga tggatcgcgt
     2401 cccggatggg tctgaaggcc actctcttct ccgggctcgg catccagatc gtggctctgg
     2461 gaatgctcgg gttcgcccct gacgcgtggc tggtggtgcc ctacgtgatg gcgtcccagg
     2521 cgctttcagg catcgccaag gacctgacca agatgagctc gaagagcgcg gtcaagctgg
     2581 tcgtccccga agactcggag tcctccctct tcaagtgggt ggcgatcctc accggctcca
     2641 agaacgcgct gaagggcgtc gggttcttcc ttggaggcct gctgttgacc gtgatcggct
     2701 tccagtccgc aatgctggtg ctcctcggga tcgtggctac cgccctggtc acgacttcga
     2761 ccctgatgga gggcagcctc ggctactcgg acatgggcgc gaagttccgc cacatgttct
     2821 cccacacccg ggagatcaac ctgctcgccg ccgcccgcct tttcctcttc gcttcacggg
     2881 acgtctggtt cgtggtcgcc ctgcccgtct atctgcggac ggtgctcgac tggagcttct
     2941 gggcggccgg cggctttctc gcgatctggg tgatcgggta cggagcggtc caggcgagcg
     3001 cccccggtct ctttgcccgc agggcgaagc agacgggcga caccccgacc ggtcgaaccg
     3061 ccgcgaggct ggccttcctg ctggccgcct tcccggccgc gatcgccgct tcgctggccg
     3121 cgaacctgga cccgaccttc gtcctggtcg tgggactgat cctcttcggc atcgtcttcg
     3181 ccctcaactc ggcggtccat tcgtatctcg tgctcgccta tgcggaggac gagacggtcg
     3241 caaagaacgt cggcttctat tacatggcga acgccggagg aaggctgatc ggcaccatcc
     3301 tctccggcgc ggtctaccag tggctcggcc tcgagggctg cctctggttc tcggtcgggc
     3361 tggtgctgat cgcggcgacg atttccctgt ggctgccctc ggaccagaaa cgcgttccag
     3421 tggaagcgat g
//
LOCUS       DAOTIP010000133         3423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32724_length_3423_cov_2.870843, whole genome shotgun sequence.
ACCESSION   DAOTIP010000133 DAOTIP010000000
VERSION     DAOTIP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32724_length_3423_cov_2.870843"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..426)
                     /locus_tag="P5138_03540"
     CDS             complement(163..426)
                     /locus_tag="P5138_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59678.1"
                     /translation="MSDSPEKAAESRTRAYGRTAGFLTIGVGLTGIFTYAYFLIASHD
                     LSKDSYGEITVLWSAVFITVSTLYRPVDPDPHDERRGNEKLDD"
     gene            complement(445..1440)
                     /locus_tag="P5138_03545"
     CDS             complement(445..1440)
                     /locus_tag="P5138_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV59679.1"
                     /translation="MKVIIQIPCLNEEETLPATLADLPREIPGVDEIETLVIDDGSTD
                     RTVEVAGECGVDHIVKLPNNRGLATGFQAGLDACLKLGADIVVNTDADNQYSGSDIPK
                     LVAPILAGEAEMVVGDREVADIEHFSPAKKVLQKFGSWVVRQLSDTEVTDATSGFRAY
                     NREAALQLLVVDNFTYTLESLIQAGKGSVAVGEVSISTNPKTRESRLFGSTSTYVRRN
                     GLAILRIYTRYEPLKVFSLLGLIATVAAVAAWMPFLVDWIANGDRSGHIQSLLLGAVL
                     FIVAIQLFALGIIGDLLAGQRVMTQRVYERVRRIELELDVEPSHQIDPPRDRSTV"
     gene            1460..2590
                     /gene="ribD"
                     /locus_tag="P5138_03550"
     CDS             1460..2590
                     /gene="ribD"
                     /locus_tag="P5138_03550"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRV59680.1"
                     /translation="MTATQPTTEADYWQRAIQLAGNGLGWTSPNPAVGAVLVKDGEVI
                     GEGWHHRHGELHAEREALADAGRRGNDPAGSTAYVTLEPCAHTGSQPPCADGLIEAGV
                     VEVVIAAEDPTEKTRGVGPSRLEEAGVKVRWAEGPVSEAALGLIQDFRKRALTGKPLV
                     TLKMAMSLDGKVATRTGDSKWISGEESRALVHRWRGEMDAVAVGSGTLIADDPRLTAR
                     HTDGGPVRQPTRVVFDSKLLATPQAALFEDVSEAPVLIIAGPDPDREKAAALAAAGAE
                     VIPVKGEGSQERFGAAMEALGERNIGSLLLEGGPNLAGAAVTAGEVDRTEIFVAPLIL
                     GGGRSATEGPGPELMEGATRVPEMRVSRVGQDVLMSSTIRSW"
     gene            2590..3207
                     /locus_tag="P5138_03555"
     CDS             2590..3207
                     /locus_tag="P5138_03555"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRV59681.1"
                     /translation="MFSGLVQDLGEVESVDLASDGARIRVKTRLSGDIALGDSIAVNG
                     CCLTATTVDGLGFTADAMQQTLDLTSIGELQAGSPVNLELALRADDRLGGHIVQGHVD
                     GTAEVLAREPDGFSLRLRISLPEGLARYVIPQGSLTIEGVSLTVADCGEGWAEVALIP
                     ETQERTNLGTLQVGSTVNIECDVIAKYVERMVSPYARGSESHTDR"
     gene            3236..>3423
                     /locus_tag="P5138_03560"
     CDS             3236..>3423
                     /locus_tag="P5138_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRV59682.1"
                     /translation="MATEDGRGSVSAAEATPFATIEEALEEIRAGRMIIVTDDEDREN
                     EGDLVMAAQFATPEAVNF"
BASE COUNT          629 a         1044 c         1178 g          572 t
ORIGIN      
        1 ggtattttac gccggcgtgt gccggaatgg ctgtcggaac gcccgctttc aaacattgtt
       61 ttaaaaacgg ttccggcgca gaaaaaagac ggtggggagg gcgcgctata tgtctatctc
      121 cggcgggaga gagactattc ttcctgaatg tgaatgcgcc gttcaatcgt ccagcttttc
      181 gtttccccgt ctttcatcgt gcgggtcagg gtcgaccggc cggtataggg tcgagaccgt
      241 gatgaacacg gccgaccaga ggacggttat ctctccatag ctgtccttgc tcaggtcatg
      301 cgaggcgatc aggaaatagg cgtaggtgaa gatcccggtg agcccgacgc cgatggtcag
      361 gaagccggcc gtcctcccgt aggcacgggt gcgtgattcg gcagcttttt cgggggagtc
      421 gctcaaggga ctgggtgagg gtgatcagac ggtgcttcgg tcacgcggag ggtcgatctg
      481 atggctcggc tccacgtcaa gctcaagttc gatccggcgc accctctcat aaacgcgctg
      541 ggtcatgacc cgctgtccgg ccagcaggtc cccgatgatg ccgagggcga agagctggat
      601 cgcgacgatg aacaggaccg cccccagcag cagcgactgg atgtggccgg aacggtcacc
      661 attggcgatc cagtcgacca ggaacggcat ccaggccgcg accgccgcca ccgtggcgat
      721 cagtccgagc agggaaaaca ccttgagcgg ttcgtagcgc gtgtagatcc ggaggatcgc
      781 caggccattg cgccgcacat aggtcgaggt cgagccgaat agccgcgact cgcgggtctt
      841 ggggttggtc gagatcgaga cctccccgac cgcgacgctg cccttgcccg cctggatcag
      901 gctttccagc gtgtaggtga aattgtcgac gaccagtagc tggagggcag cttcccggtt
      961 gtaggcccgg aaaccggagg tggcgtcggt cacctcggtg tcggaaagct ggcggacgac
     1021 ccaggaaccg aacttctgga gcaccttctt ggccggtgag aaatgttcga tgtcggcgac
     1081 ctcgcgatca ccgaccacca tctccgcctc tccggcgagg atcggggcaa ccagcttcgg
     1141 gatgtcgctt cccgagtact ggttgtcggc gtcggtgttc acgacgatgt cggcgccgag
     1201 cttcaggcag gcgtcgagcc ccgcctggaa gccggtggcc agtccccggt tgttcggcag
     1261 cttgacgatg tggtcgaccc cgcactctcc ggcgacctcg accgtgcggt cggtcgagcc
     1321 gtcgtcgatc acgagggttt cgatctcgtc caccccggga atctcccgcg gcaggtccgc
     1381 gagagttgca gggagagtct cctcctcgtt cagacagggg atctggatga ttaccttcat
     1441 tagccttcgg gattctccca tgaccgccac ccaacccacc acagaggccg actactggca
     1501 aagggccatc caactggccg gcaacgggct gggctggacc agccccaatc cggcggtcgg
     1561 tgcagtgctg gtcaaggacg gtgaggtgat cggggagggc tggcatcacc gccacggcga
     1621 actgcatgcc gaacgcgagg ccctggcgga tgcgggacgc cgtggcaacg acccggccgg
     1681 ctcgaccgcg tacgtgaccc tggagccctg tgcccacacc ggcagccagc ccccttgtgc
     1741 tgacggactg atcgaggcgg gggtcgtcga ggtcgtgatc gcggccgagg acccgaccga
     1801 gaagacgagg ggggtcggtc cgtcccggct cgaggaagcg ggcgtcaagg tgcgctgggc
     1861 ggaaggtccg gtaagcgaag cggcgctcgg attgatccag gatttccgca aacgggcact
     1921 gaccggcaag ccgctggtca ccctgaagat ggcgatgagc ctggacggca aggttgccac
     1981 caggaccggt gactcgaaat ggatctccgg cgaagagagc cgcgccctgg ttcaccgctg
     2041 gaggggcgag atggatgcgg tcgcggttgg ttcgggcacg ctgattgccg acgacccgcg
     2101 gctcaccgca cgtcacaccg acggcggtcc ggtccgccag ccgacccggg tggtcttcga
     2161 ctcgaaactt ctcgccaccc cgcaggccgc actcttcgag gatgtgtccg aggcacccgt
     2221 cctgatcatc gccggcccgg acccggatcg ggaaaaggcg gcggcgctgg ccgcggccgg
     2281 ggcggaagtg atcccggtca agggcgaagg ttcccaggaa agattcggcg cggcgatgga
     2341 agcgctcggc gaacggaaca tcgggtcgct gctgcttgaa ggcggcccga acctggccgg
     2401 ggcggcggtc acggcggggg aggtcgaccg caccgagatc ttcgtcgccc cgctcatcct
     2461 cggcggtggc cgctccgcga ccgaaggtcc gggacccgag ctgatggagg gggcgaccag
     2521 ggtgccggag atgcgggtga gccgggtcgg gcaagatgtg ctcatgagct cgacgatcag
     2581 gagctggtga tgttctccgg actcgttcag gacctgggtg aggtcgaatc ggtcgatctc
     2641 gcttccgatg gcgcccggat cagggtgaag acccgtcttt cgggggacat cgcgctcggc
     2701 gactcgatcg ccgtcaacgg ctgctgcctg accgccacga ccgtggacgg tttggggttt
     2761 accgccgacg cgatgcagca aacccttgac ctcacctcga tcggcgaact tcaggcgggc
     2821 tcgccggtca atctcgaact ggccctgcgg gccgacgacc ggctcggcgg acacatcgtc
     2881 cagggccacg tcgacgggac cgccgaggtg ctggcgcggg agccggatgg cttctcgcta
     2941 cgcctcagga tctcgcttcc ggaaggactt gcccgatacg tgatccccca gggatcgctc
     3001 acgatcgaag gcgtcagcct caccgtcgcc gactgcggag aaggctgggc cgaggtggcg
     3061 ctgatcccgg aaacgcagga aagaaccaat cttgggaccc ttcaggtcgg cagcaccgta
     3121 aacatcgagt gtgacgtaat tgccaaatac gtcgagcgca tggtgtcacc atatgcacga
     3181 gggtccgaaa gccacacgga ccgttgaatc ccgaatcaga aagtctgaaa agagaatggc
     3241 taccgaagat gggcgcggca gcgtgagtgc cgccgaagcg actccgttcg cgacgatcga
     3301 agaggccctg gaggagatca gggccggccg gatgatcatc gtcaccgacg acgaggaccg
     3361 ggaaaacgaa ggcgacctcg tgatggcggc ccagtttgcc accccggagg cggtcaactt
     3421 cat
//
LOCUS       DAOTIP010000134         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_32736_length_3422_cov_3.676567, whole genome shotgun sequence.
ACCESSION   DAOTIP010000134 DAOTIP010000000
VERSION     DAOTIP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32736_length_3422_cov_3.676567"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="P5138_03565"
     CDS             complement(<1..205)
                     /locus_tag="P5138_03565"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV59683.1"
                     /translation="MPALRSKTVTHGRNMAGARALLRASGVAREDFGKPIVAVANSYT
                     QFVPGHTHLKPVGEVVSEAIKDAG"
     gene            complement(222..1247)
                     /locus_tag="P5138_03570"
     CDS             complement(222..1247)
                     /locus_tag="P5138_03570"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRV59684.1"
                     /translation="MTITETSAATTIPHDYSKRAMVFGGRSSQDLAAKIAGKLSLSVG
                     DVTLKTFSDGEIYGRYEESIRGADVFLVQSTCANVETGMTPNDALMELMIMADAAQGA
                     SAHRIIAVMPWFGYARQDKKSAPREPITSRVVARSLEAVGIDRVLTMDLHAGQIQGFF
                     TIPVDHMTAQPMLTQWFTDQYDPSELVIVSPDAGRAKAARNFARKVGTEWAIMEKERP
                     KQQVAEIGYVVGDVKDKIAIISDDMIDTAGTLCAAARTVLDEGAKSVIACATHGVFSG
                     PAFERLPYEKSGLEKIVVTDTIPLRPGAPDNIHVLSTAGTLADSIRRIFTDDSVSEIF
                     AGENQLF"
     gene            complement(1244..2647)
                     /gene="glmU"
                     /locus_tag="P5138_03575"
     CDS             complement(1244..2647)
                     /gene="glmU"
                     /locus_tag="P5138_03575"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRV59685.1"
                     /translation="MTGPPVTALIMAAGQGTRMRSALPKVLHPVCGRPMIAWPVLAAR
                     EAGADRVAVIVSPERDLSDALPDGVETVEQVESNGTGGAVRSALEIVEKSPRVLVLSG
                     DVPLISAEVITGLLASQEESGASATVMTAILDDPGTYGRIVRGGDGTIRKIVEAKSEG
                     DATREELAIREINSGTYVFDGESLASALGELSADNAQGEYYLTDVIAVMRSRGELVTA
                     HVSKDPAINLGINNRADLAVVETEARSRILRQHMLDGVTIVDPATTWIDAGVTIGQDA
                     RIEPGTMLSGATEIGEEAVIGPHSTIIDSGIGRGCHVIQSYLNLCELAEGCSVGPFAY
                     LRPGAKLEDGAKAGTFVEVKNSTIGPGSKVPHLAYVGDTDIGSNSNLGAGTITANYDG
                     REKHRTKIGSDVKIGVDTMLVAPVEVGDGAYTGAGAVIRDNIPPGALSVSENDQRNVD
                     DYAERLAARKQEGETSQ"
     gene            complement(2754..3311)
                     /locus_tag="P5138_03580"
     CDS             complement(2754..3311)
                     /locus_tag="P5138_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRV59686.1"
                     /translation="MEYNAAQEKEVKRASGMFTGIGILMILVGCFAIVAPHIVGQAFA
                     LLVGLAFIIASVFIFVWAFSAEGGAAVIGRIAWALVALIAGLIIVTHTDLSAKAFTVI
                     LIAYFLAAGVVRLAAAFAQRGVPGAGWLGLNGALSLLIGLIFVVSWPNSAEWAIGLLI
                     GIDLIFSGWTLILLSQEAKSEILDT"
     gene            3364..3420
                     /locus_tag="P5138_03585"
     tRNA            3364..3420
                     /locus_tag="P5138_03585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3389..3391,aa:Gln,seq:ttg)
BASE COUNT          600 a         1081 c         1135 g          606 t
ORIGIN      
        1 cgcccgcatc cttgatcgcc tcggagacga cctcgccgac cggcttcagg tgagtgtggc
       61 cgggcacgaa ctgggtgtag gagttggcca ccgccacgat cggcttgccg aaatcctcgc
      121 gggccacacc ggaagcgcgc agaagcgccc gggccccggc catgttgcgt ccatgggtaa
      181 cggtcttgga tctgagcgct ggcatgcgct gaggttactc cctagaagag ctggttttcg
      241 ccggcgaaga tctcggagac cgagtcgtcg gtgaagatgc gacggatcga gtcggcgagc
      301 gttccggcgg tgctgagcac gtgaatgttg tccggggcac cggggcggag cgggatcgtg
      361 tcggtgacga cgatcttctc gagacccgac ttctcgtagg gcagacgctc gaaggccggg
      421 ccggagaaaa caccgtgggt ggcgcaggcg atcactgact tggctccctc gtcgaggaca
      481 gttcgagcgg cggcgcaaag ggtgccggcg gtgtcgatca tgtcgtcgga gatgatcgcg
      541 atcttgtcct tgacgtcacc gaccacatag ccgatctccg cgacctgctg tttcggccgt
      601 tccttctcca tgatcgccca ctcggtcccg actttgcggg cgaagttgcg ggcggccttg
      661 gcgcgaccgg cgtccgggga gacgatcacc agttccgacg ggtcgtactg gtcggtgaac
      721 cactgggtca gcatcggctg ggcggtcatg tggtcgaccg ggatcgtgaa gaaaccctgg
      781 atctggccgg cgtgaagatc catggtcagg acccggtcga tgccgaccgc ttccagggac
      841 cgggccacga cacgggaggt gatcggctca cgaggtgccg acttcttgtc ctgacgggca
      901 tatccgaacc agggcatgac ggcgatgatc cggtgggccg aagcaccctg ggcggcgtcg
      961 gccatgatca tcagctccat cagggcgtcg ttcggggtca tcccggtctc gacgttggcg
     1021 caggtcgact ggaccaggaa gacgtcggcg ccgcggatcg actcctcgta acggccgtaa
     1081 atctcgccgt cggagaaggt cttcagggtg acgtcaccga ccgagagcga aagcttgccg
     1141 gcgatcttcg ccgcgagatc ctgcgaagaa cggccgccga agaccatcgc acgcttggaa
     1201 tagtcgtgcg ggatcgtggt ggctgcagaa gtctcggtaa tggtcactga ctggtttccc
     1261 cttcttgttt ccgggcggca agccgctcgg cgtaatcatc aacgtttctc tggtcgttct
     1321 cgctaaccga gagcgcaccg ggcggaatgt tgtcgcggat caccgcgccg gcgccagtgt
     1381 acgcaccatc gccgacttcc accggtgcaa caagcatcgt gtcgacaccg atctttacgt
     1441 cagagccgat cttcgtgcgg tgcttctccc ggccgtcgta gttggccgtg atcgtgccgg
     1501 ctcccagatt cgagttcgag ccgatgtccg tgtcaccgac ataagcgagg tgcggcacct
     1561 tggaacccgg cccgatggtc gagttcttga cctcgacgaa ggtccccgcc ttggccccgt
     1621 cctccagctt cgcccccggc ctcaggtagg cgaagggacc gaccgagcag ccctcggcca
     1681 gctcgcagag attcagatag gactggatca cgtgacagcc ccggccgatc ccggaatcga
     1741 tgatcgtgga atgcggcccg atcaccgctt cttccccgat ctcggtcgcg ccggagagca
     1801 tggtcccggg ctcgatccgg gcatcctggc cgatcgtcac gccggcgtcg atccaggtgg
     1861 tggccgggtc gacgatggtc accccgtcaa gcatgtgctg cctgaggatg cggctccttg
     1921 cctccgtctc gaccacggcc agatcggccc ggttgttgat cccgaggttg atcgccggat
     1981 ccttcgagac atgggcggtg accagttccc cgcgggaacg catcaccgcg atcacgtcgg
     2041 tcaggtagta ctcgccctgc gcgttgtcgg cggaaagctc accgagcgcc gaggcgagtg
     2101 actcgccgtc aaagacatag gtgccggagt tgatctcccg gatcgccagc tcctcacggg
     2161 tcgcgtcacc ttcggacttc gcttcgacga tcttgcggat cgtgccgtcc ccgccgcgga
     2221 ctatccggcc ataggtgccg ggatcgtcga ggattgcggt catcacggtg gccgaggcgc
     2281 cgctctcttc ctgggaggcg aggaggccgg tgatcacctc ggccgagatc aggggcacgt
     2341 ccccggagag gaccaggacc ctgggtgact tctcgacgat ttcgagggcg gaacggaccg
     2401 cccctccggt gccgttcgat tcgacctgct cgaccgtttc caccccatcc ggcagggcat
     2461 cggaaaggtc cctttcgggg gagacgatca ccgcgacccg gtcggcgccc gcctcgcggg
     2521 cagccagcac cggccaggcg atcatcggtc gaccacagac ggggtgaagc accttcggca
     2581 gggcggaccg catgcgggtg ccctgaccgg ccgccatgat cagcgctgtt acgggaggcc
     2641 cggtcacaga atcattctaa gtcgggcggg gtccgacccg ccctccgagt caaaccagcc
     2701 ggtccacctg gttcacaaat ggctggaccg gctccgctca ttggcgattc ggttcaggta
     2761 tcgagaatct cggacttggc ttcctgcgag agaaggatca gggtccagcc ggagaagatc
     2821 agatcgatgc cgatcaggag cccgatcgcc cattcggcgg aattgggcca ggagaccacg
     2881 aagatcaggc cgatcagaag ggaaagtgcc ccgttcagcc cgagccatcc ggcgccggga
     2941 acgccacgct gcgcgaaggc ggcggcgagc cggaccacgc cggcggcgag gaagtaggcg
     3001 atcaggatca ccgtgaaggc cttggcgctg aggtccgtgt gggtgacgat gatcaatccc
     3061 gcgatcagcg cgacgagagc ccaggcgatc cggccgatga ccgcagcgcc cccttccgcc
     3121 gagaaggccc agacgaagat gaatacggac gcgatgatga aggccagccc gaccagaagg
     3181 gcaaaagcct gcccgacgat gtggggcgcg acgatcgcga aacatccgac caggatcatc
     3241 aggattccga tcccggtgaa cattccggag gcccgcttca cttccttctc ctgtgctgcg
     3301 ttgtattcca tgaccaaagc ctatccgcgc ctgcggaggt agagtcaggc tcgattccgg
     3361 ggtggtgtaa tcggtagcac accgggtttt ggtcccggca gtggaggttc gagtcctccc
     3421 tc
//
LOCUS       DAOTIP010000135         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33031_length_3398_cov_3.766378, whole genome shotgun sequence.
ACCESSION   DAOTIP010000135 DAOTIP010000000
VERSION     DAOTIP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33031_length_3398_cov_3.766378"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="P5138_03590"
     CDS             <1..868
                     /locus_tag="P5138_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59687.1"
                     /translation="KLKRLWDRPAFSNVVALMALFVALGGASYAAIKVPKNSVGTRQI
                     KNRAVTAAKLRKNSVNSAKVRNRSLRAVDFRKGQLPRGATGPLGPVGPPGATGLAGEI
                     GPTGLQGAIGLPGATGEAGPQGVTGEAGPQGVTGDAGPQGATGETGATGATGLSTTAV
                     MTGYTSLPTGGSRVFGVSGVTSSADPNSPFPVVMRTPGVAVTAANLSVSVDVAPGTNA
                     NRYFALTKVSQTGELDPTFVACNISNSSTACSPTVTSVVIPANTLIVMYTASTFGSPA
                     ATGVHFGFTIGP"
     gene            905..1168
                     /gene="ffs"
                     /locus_tag="P5138_03595"
     ncRNA           905..1168
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5138_03595"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1206..1652
                     /locus_tag="P5138_03600"
     CDS             1206..1652
                     /locus_tag="P5138_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HRV59688.1"
                     /translation="MSDREWKVYLAGEIHSDWRGRVEQACEDLPVAFTAPVTDHPASD
                     AAGTGTLGEEEKKFWADHKGAGVNSIRTQTFIQEADVVVVRFGDKYRQWNAAFDAGFA
                     SALGKPVITQHDEELDHALKEVDAAARATARTPEQVAAVLRYVITA"
     gene            1745..>3398
                     /locus_tag="P5138_03605"
     CDS             1745..>3398
                     /locus_tag="P5138_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59689.1"
                     /translation="MKKRANRKVGAWATALGLTLLSCLALTSAASAQGIVNPAADRAY
                     NVQPVDFRGNASLNAIDFFKYSHEPYMQLYYTEQVKWNDEHGGHLQNRFAQQTRVGTG
                     EDTIDIDGDWNIPMADVTNRLQYELTPTNFPGEFRVSTSPLPNGAPLQDGKYDMVVTQ
                     FYTEWPYSLSYGPNDPIQKKWIAAFWIDTGIPDTVIDTEVPSGSTAANSRTFTFHAQD
                     VNPTTSSGTILNCRMDGGPWTECNDPLAVQYTPTRPGSYSVSNLTPGQHTFQVRANDA
                     AGNFDPTPATSTFYVDGTPPTINIVRPADGNRFVLGSDFNSDFTCTDPLVGTPPGNSG
                     IKTCAGPAKVDTSTLGSHTFTVNAEDNAGNTASKTTTYFVDPPKYTDVIHSGDPIAYY
                     RLGDPVGSTTMTDSSGNGHHGELKNGVKLEQPAAPACQKRPRPAVCSLSDEKEDFAAD
                     FPERDGYGYANGIMAPQNAYSIEAWVKPYGTGDGSIAGQGGGGQLFIKDGKLALRQTQ
                     DTVQSPGPLLAPGQWWYVAATWDGQVTRLYVNGEEVASSDSVN"
BASE COUNT          686 a         1117 c         1041 g          554 t
ORIGIN      
        1 gaagctgaaa aggctgtggg accgtcccgc cttctcgaac gtggtcgcgc tgatggcttt
       61 gttcgtggct ctcggtggtg cttcgtacgc ggcgatcaag gtcccgaaaa acagcgtcgg
      121 aaccaggcag atcaagaatc gggcggtgac ggcggccaag ttgaggaaaa actcggtcaa
      181 ctcggccaag gtcaggaacc gctcgctgcg agcggtcgat ttcaggaagg gacagctgcc
      241 gaggggggcg actggccccc tcggaccggt ggggccgcct ggcgcgaccg gactggccgg
      301 cgaaatcggc ccgaccgggc ttcagggagc gatcggactc ccgggagcta ccggagaggc
      361 aggcccccag ggcgtgaccg gcgaggcggg ccctcagggc gtgaccggtg acgcaggtcc
      421 tcagggcgcc accggtgaaa cgggagcaac cggtgcgacc ggactcagta ccaccgccgt
      481 catgaccggg tacacgagtt tgcccaccgg cgggagccgg gtcttcggtg tctccggggt
      541 cacctccagt gcagatccaa attccccgtt cccggttgtc atgcgtacac cgggggtggc
      601 ggtcaccgcc gcgaaccttt ccgtttctgt ggatgttgcc cccgggacca acgcgaacag
      661 atacttcgcg cttacgaagg taagtcagac cggggagctc gacccgacgt tcgtcgcctg
      721 caacatcagc aactcatcca cggcctgcag ccccacagtg acatccgtgg ttattccggc
      781 gaacactctg atcgtgatgt acaccgcgtc gaccttcggg tccccggctg cgaccggggt
      841 ccacttcgga ttcacgatcg gtccctgacc cggtcgccgc tcgcacgcta ggattcctgg
      901 tggaccgtgc taggcgggga gtcagcggtg ccctgcactt gcaatccgct ttagcaaggc
      961 cgaatgccgt tctgagggtc tggccatcgg ggtcagtaca ccgagggagg cgttgacggt
     1021 caggtcccgc gcgacgcaag cccgtgaacc aggtcaggtc cggaaggaag cagccttaag
     1081 cggaaactac gggtgccgcg ggtgagccta acccgagcca aactcgacgg acacgcccga
     1141 tggcttcgtt tcgaagacgg atgcacggtc cattttcaac cttccattgc gagtaccgtg
     1201 ggcatatgag cgatcgtgaa tggaaggtct acctcgcagg tgaaatccac tccgactgga
     1261 ggggccgggt cgaacaggcc tgtgaagacc tgccggtcgc tttcacggcc ccggtcaccg
     1321 accaccccgc ctcggatgcg gccggcaccg gcacgctcgg ggaagaggag aagaagttct
     1381 gggcggacca caagggggcc ggggtcaact cgatccgcac ccagaccttc atccaggagg
     1441 ccgatgtggt ggtggttcgt ttcggcgaca agtaccgcca gtggaacgcc gcctttgacg
     1501 ccggattcgc ttcagcgctg ggaaagcccg taatcaccca gcacgatgag gagctcgatc
     1561 acgccctcaa ggaagtggac gccgccgccc gcgccaccgc ccgcaccccg gagcaggtcg
     1621 cggccgttct ccggtacgtg atcaccgcct gaacataatc ttcacacggg tcttaccgac
     1681 tggtgtacgg tacaaaccga atgatgtgcg gtaagactga atcgacatgt tgggggacgg
     1741 caagatgaag aagcgggcaa accggaaggt gggcgcatgg gccactgcgc tcggtctgac
     1801 acttctgtca tgcctggctc tgacctcggc tgcttccgcc cagggaatcg tcaatccggc
     1861 cgccgaccgc gcctacaacg tccagccggt ggacttcaga ggcaatgcca gcctcaacgc
     1921 gatcgacttt ttcaagtact cccacgagcc ctacatgcag ctctattaca cagagcaggt
     1981 gaagtggaat gacgagcacg gcggccacct ccagaaccgg tttgcacagc agacgagagt
     2041 ggggacgggc gaagacacga ttgatatcga tggtgactgg aacattccca tggccgacgt
     2101 gaccaatcgc cttcaatacg aattgacccc taccaatttt cccggagagt tccgggtttc
     2161 gaccagtccc cttccgaacg gggcgccgct tcaggatggc aagtacgaca tggtcgtaac
     2221 gcagttctac accgagtggc cgtactccct tagctacggt cccaacgacc cgatccagaa
     2281 gaaatggatt gccgcattct ggatcgacac cggtatcccg gacacggtca tcgacaccga
     2341 ggtcccgtcg ggttccacag ccgccaatag ccggaccttc accttccacg cgcaggatgt
     2401 gaacccgacc acttcatcgg ggacgatcct caactgccgc atggacggtg gcccctggac
     2461 cgaatgcaat gaccctctcg cagtccagta caccccgacc cggcccggct cctactcggt
     2521 cagcaacctg accccgggcc agcacacctt ccaggttcgg gccaatgacg cggcaggcaa
     2581 cttcgacccg accccggcca cctcgacttt ctacgtcgac ggaaccccgc cgacgatcaa
     2641 catcgtccgc ccggctgacg gaaaccggtt cgtgcttggc agtgacttca actcggactt
     2701 cacctgcacc gatccgctgg tcggaacacc tcccggcaac tccggaatca agacctgcgc
     2761 cggtcccgcc aaggtcgaca cctcgaccct aggcagccac accttcacgg tgaacgccga
     2821 ggacaacgcc ggcaacaccg ccagcaagac caccacctac ttcgtggacc cgccgaagta
     2881 caccgacgtg attcactcgg gtgacccgat cgcctactac cgcctaggtg atccagtcgg
     2941 ttcgaccacg atgactgaca gctccggcaa cgggcatcac ggagaactca agaacggcgt
     3001 caagcttgaa cagcctgccg cgcccgcctg ccagaagcgg ccccgacccg cggtctgttc
     3061 gctgagcgac gagaaggagg acttcgccgc cgatttcccg gagcgtgacg ggtacggcta
     3121 cgcgaacggg atcatggccc cgcagaacgc ctactcgatc gaggcctggg tcaagccgta
     3181 cggaaccggg gatggttcga tcgccgggca gggcggagga ggccagctct tcatcaagga
     3241 cggaaaactg gcgctgcgcc agacccagga cacggtccag tcacccggtc cgctcctggc
     3301 gccgggccag tggtggtacg tcgccgccac ctgggacggc caggtgacca ggctctacgt
     3361 caacggcgaa gaggtcgcct cctctgactc cgtgaaca
//
LOCUS       DAOTIP010000136         3381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33238_length_3381_cov_3.949188, whole genome shotgun sequence.
ACCESSION   DAOTIP010000136 DAOTIP010000000
VERSION     DAOTIP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33238_length_3381_cov_3.949188"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..325)
                     /locus_tag="P5138_03610"
     CDS             complement(65..325)
                     /locus_tag="P5138_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV59690.1"
                     /translation="MNRYRIELRPAALRSLRKLDPQIRGRVQGAIALLADDPRPPSAK
                     KLKGREAFRVRVGDYRVIYTVEDDVLLVVVVTLGHRRDVYER"
     gene            complement(322..570)
                     /locus_tag="P5138_03615"
     CDS             complement(322..570)
                     /locus_tag="P5138_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV59691.1"
                     /translation="MSTFSISKARESLSDAVELSRTEAVFLERHGKKAAVMVSPERYE
                     EMMEALEEIEDVAAFDQAMAEEGPNIPWDEAKADLGWD"
     gene            complement(668..741)
                     /locus_tag="P5138_03620"
     tRNA            complement(668..741)
                     /locus_tag="P5138_03620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Arg,seq:acg)
     gene            845..1156
                     /locus_tag="P5138_03625"
     CDS             845..1156
                     /locus_tag="P5138_03625"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRV59692.1"
                     /translation="MSIVKINAITVPSERFDEFAERFASRAGKVEEAEGFESFDLLRP
                     NDEREVCLVVTRWRSEDDFRAWLNSPDFAAGHSQHREQGPVGTSSEIWSFDVLESITA
                     D"
     gene            complement(1184..2938)
                     /locus_tag="P5138_03630"
     CDS             complement(1184..2938)
                     /locus_tag="P5138_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HRV59693.1"
                     /translation="MALLILFGFIAGAATAVSPCVLPVLPVALSAGATGGRRRPAGIV
                     AGLTASFTFATVALVYLIDALGLPDDLIRNLAIAVLIGFGLVLLLPAVSSRLEGWLSR
                     FTARFTPKTEADGFWSGTLVGASLGLVYAPCAGPILAGVITVSASQSFTVDRLAIAFA
                     YALGSGLVLYLLMLGGRRLIAPLSRRVGWLQAATGIVMIVFGIGMAAQWDLKFQNWIA
                     DDLPAVLVNPTKDLESSDASISRLADLRAGHTTKSAEQIAAGAAAVQSEARAASDLPV
                     IGRAPEFQGTQQWFNTPGDRPLTMAGLRGRVVLIDFWTYTCINCIRTQPWLNAMNERY
                     GDRGLTIVGVHTPEFPFEKDAGNVEDAIKRAGIEYPVAQDNDYTVWNSYGNQYWPAEY
                     FVDADGNIRYAHFGEGDYGHKEEVIRELLAEAGENPGAGKTGATGMKAAHVLLTPESY
                     LGSARADRFENGYILPGVHDFGPLKEPRLDWLAYGGKWSIGPEKARSDGGEIAVHFRA
                     KRVYLVLSSPGRSRTAGIELDGKPVNATAAGGDVKGGEVKVTGERLYSLIDLPEPGRH
                     LLTVSPESGIRGFAFTFG"
     gene            3000..>3381
                     /locus_tag="P5138_03635"
     CDS             3000..>3381
                     /locus_tag="P5138_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59694.1"
                     /translation="MPDTRNYANLTRRYAKFSLAGVLALLALVVLVVGGSPDKAQAAQ
                     VKCPQFRVLNNDRIGKVSFPAGYYNVTLLNGNKLSCAGSTKLFQEFLQDWDGRLRSPW
                     RVYLSGNKRVFRAGNTDKGFTATPA"
BASE COUNT          628 a         1119 c         1054 g          580 t
ORIGIN      
        1 ggcaagggcc gggtcccgtg ggccaggaag cgtgatggtt gcttgtttgc ccgcggtccc
       61 gccgctaccg ctcgtagaca tctcgccggt gaccaagcgt gaccacgaca acgagaagta
      121 catcgtcttc aacggtgtag atcacccgat agtcacccac tcggacccgg aaggcttcac
      181 gtcctttcag cttcttcgcg ctcggcggcc gggggtcgtc ggctaggagg gcgatggctc
      241 cctggactct tcctctgatc tgcggatcaa gctttcgtag ggatcgaagt gcggccgggc
      301 gcagttctat ccggtaacga ttcaatccca gcccaggtcg gctttggctt catcccacgg
      361 gatgttcggg ccttcttcgg ccatcgcctg atcgaaggca gcaacgtctt cgatctcctc
      421 gagcgcctcc atcatctcct cgtagcgctc gggactgacc atgaccgcag ccttcttgcc
      481 gtgccgctcg agaaaaacgg cttcggtccg ggatagctcg accgcgtccg agagagactc
      541 ccttgccttg ctgatgctga aagtggacat gagagaattg tacagtttga tgatctattt
      601 gtacagatca ggccgaaacc agcaattgcg acatgactcc gtcgcaggaa ccaagccacg
      661 agacgaacgc gcccgacagg attcgaacct gtgacctttg gtttcgtaga ccaacgctct
      721 atccagctga gctacgggcg cgggacgctg ggggtaaacc ccggacgggc attgtatggg
      781 tcagaacctg atcgcgttct tccgccggcg tggctcgatt ctcgcgcctc cgtaggatcg
      841 agttatgagc atcgtcaaga tcaacgcaat aacagtcccc agcgagcgat tcgacgaatt
      901 cgccgagcgc tttgcctccc gcgccggcaa ggtcgaggaa gcggaaggct tcgagtcctt
      961 cgacctgctc cgccccaacg acgagcgcga agtctgcctg gtggtcaccc gctggcgatc
     1021 cgaggacgac ttcagggcgt ggctcaacag tccagatttc gccgccggcc attcccagca
     1081 ccgcgaacag ggaccagtcg gcacctccag cgaaatctgg tcctttgacg tgctcgaatc
     1141 gatcaccgcc gactgagcaa tcgccggggt aactgcgacc ggctcaaccg aaggtgaagg
     1201 cgaaaccgcg gatccccgac tcggggctca cggtcagcag atgccggccg ggttccggca
     1261 gatcgatcaa ggagtagagc cgctcgccgg ttaccttcac ctcgccaccc ttgacgtctc
     1321 caccggcggc cgtggcgttg accggcttgc cgtcgagttc gatgccggcg gtgcgggacc
     1381 tgccgggtga ggagagcacc aggtagaccc gcttggcccg gaaatgcacg gcaatctcac
     1441 ccccgtccga tctcgccttc tccgggccaa tgctccactt gccgccgtag gccagccagt
     1501 cgagtctcgg ctccttcagc gggccgaagt cgtggactcc cggcaggatg tacccgttct
     1561 cgaaccggtc ggcccgggcc gagcccaggt agctctccgg ggtcagcaga acgtgggccg
     1621 ccttcatgcc ggtcgctccg gtcttcccgg cccccgggtt ctcccccgcc tcggcgagca
     1681 actcgcggat cacctcttcc ttgtggccgt aatccccttc cccgaaatgg gcgtagcgga
     1741 tgttgccgtc ggcatcgacg aaatactcgg ctggccaata ctgattgccg taggaattcc
     1801 agaccgtgta gtcgttgtcc tgggccaccg ggtactcgat gccagcccgc ttgatcgcat
     1861 cctcgacgtt gccggcgtcc ttttcgaagg ggaactccgg ggtgtgaact ccgacgatgg
     1921 tgagcccccg gtctccgtag cgctcgttca tcgcgttcag ccacggctgg gtccggatgc
     1981 agttgatgca ggtgtaggtc cagaagtcga tcagaaccac ccggccgcgc agaccggcca
     2041 tggtcagcgg ccggtcaccg ggggtgttga accactgctg ggtgccctgg aactccggag
     2101 cccggccgat caccgggaga tctgacgccg ccctcgcctc gctctgaacc gcggcagcgc
     2161 cggccgctat ctgctcagcc gacttcgtcg tatgaccagc ccgcaggtcg gccaggcgcg
     2221 aaatcgaggc gtcgctggac tcgagatcct tcgtggggtt gaccagcact gccggcaggt
     2281 cgtcggcaat ccagttctgg aacttcaggt cccactgcgc cgccatcccg ataccgaaga
     2341 cgatcatcac gattcccgtc gccgcctgaa gccaaccaac cctgcggctc agcggggcga
     2401 tcaatcggcg gccgcccagc atcagcaggt agaggaccag ccccgaaccg agcgcgtagg
     2461 cgaaggcgat cgccagccgg tctacggtga acgactggga agcggagacc gtgatgaccc
     2521 cggccaggat cggcccggcg cagggcgcat agaccaggcc gaggctggct cccaccaggg
     2581 tgcctgacca gaaaccgtcc gcttccgtct tcggggtgaa cctggcagtg aagcgactga
     2641 gccagccttc cagcctggaa gaaacggccg gcagcagcag gacgaggccg aagccgatca
     2701 gaacggcgat cgccaggttg cggatcaggt catcgggcag gcccagcgca tcgatcaaat
     2761 agacgagggc caccgtggcg aaggtgaacg aggcggtcag cccggccacg atcccggccg
     2821 gacgacgcct gccaccggtg gcgccggcgg aaagcgccac cgggagaacc ggcagcacgc
     2881 agggcgagac cgcggtagcg gccccggcga tgaacccgaa aaggatcaac agcgccatac
     2941 cggaagattc gcagacaggc cacaccggaa cttgaccgtc cgggtagggt tttgaggaca
     3001 tgcccgatac ccggaattac gcgaatctga cccgtcgata cgccaagttc tccctcgccg
     3061 gagttctggc cctgctggcc ctcgtggtgc tggtggtcgg gggcagcccc gacaaggcac
     3121 aggcggcgca ggtcaagtgc ccgcagttcc gggtcctgaa caacgaccgc atcggcaagg
     3181 tttccttccc ggccggctat tacaacgtca ccctgctgaa cggcaacaag ctcagctgtg
     3241 caggctcgac gaagcttttc caggagtttc tccaggactg ggacggccgg ctccgcagcc
     3301 cgtggcgggt gtacctgagc ggcaacaagc gggtcttccg ggccggcaac acggacaagg
     3361 gcttcaccgc caccccggcc a
//
LOCUS       DAOTIP010000137         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33425_length_3367_cov_3.056461, whole genome shotgun sequence.
ACCESSION   DAOTIP010000137 DAOTIP010000000
VERSION     DAOTIP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33425_length_3367_cov_3.056461"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1995
                     /locus_tag="P5138_03640"
     CDS             277..1995
                     /locus_tag="P5138_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRV59695.1"
                     /translation="MSEITGGELFARCLASEGIEHVFGLPSPEIDPLFAQLEKYGIRL
                     VPVRHESAGVHMAEGLYKSTGKASCVIGNPGPGAANLLPGIFTALHEGVPVLAITSQH
                     RMGVSYPTPPSTFQGQDQLDSFQPAVKWGGPIFEWARIPEVLRLAFREMWNGRPGPVH
                     VDVPVTVMYDLGEEDEQPILEPEAYRPSQPQASAAQIEAAADLLESARRPVVVAGAGV
                     DRAGANAEAAELAEVLGCPLIPTQAGRASARADHPNYVFGFGEGGIKARLDADVILVA
                     GSRIGNLDLPYDKYWGEPGTVKLIQIDTDPSNIGVTRPLTMGIVADAADALGGIAAEL
                     KRRGKSPSEEATAALAEARETDARWHASQASTADEWEGEGIHPARAIEVIGETFGDQA
                     IYTVDGGLTAVWAYAGLPPTGPRSYHSILELGMLGTGIPSAIGSKIGQPDREVVCVTG
                     DGAAGFNIMEMQSAVRDSIPVTVIVFAEGAWTMEEPNELALFGHTFGTEQGEIRWDMV
                     AEGLGCHGEFVDQLDDLGPALGRCRDSGQPSVVCLKTDRDANRVIPPAMAARWMEVQA
                     GPIEGV"
     gene            complement(2052..2801)
                     /locus_tag="P5138_03645"
     CDS             complement(2052..2801)
                     /locus_tag="P5138_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59696.1"
                     /translation="MSFAEKVVLITGGANGFGRATGARVAGEGAHVVLADIEEEAGRE
                     AAAEIGAEFIHCDVRDPDASEAAVDFAVSRFGGLDIAFLNAGISTGCGLVESFDLELY
                     RRAMQINLDGVVFGINAAVPAMRKRGGGAIVATASLAGLTSVPFDPIYAANKHGVVGL
                     VRSAGPVYELENIRINGICPGFADTRIIDGFKEGLVSEGIPIIEVEHVVNGIVDLMVS
                     PESGVCHFVQAGMDPQEFRFRNIPGPKAAEA"
     gene            2884..>3367
                     /locus_tag="P5138_03650"
     CDS             2884..>3367
                     /locus_tag="P5138_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV59697.1"
                     /translation="MNPNLATLLTESAEQFPERTAIRLEDTLIPYAGLDQTSKLVAGL
                     LQSRGVETGDRVGVMLPNVPHFPMVYYGVLRMGARVVPMNVLLTAREIKHYLGNSGAK
                     LVVVWEDFAAAAVEAAEELGVDVISISPTNVGDLIQGIEPYEDIVEVEPDDTAVILYT
                     S"
BASE COUNT          604 a         1134 c         1096 g          533 t
ORIGIN      
        1 cgctcggacc ggttgccatc cggcttcagg ccgagatcaa gaaggtcttc gacccgaagc
       61 tgctgctcaa cccgggaaag aagatcctcc tgccctgaag tcctccggtc cggacatgcc
      121 cacggttcga acggctgcgg gaaaaccctg aactttcgct cggtgagcgg aaaacgggga
      181 tttgctccta cactcggaat tgccttcttt cggaagagaa gcaggcttcc acccacgtgc
      241 ggcaccgcac ggtgcctcag acaacaagga gcagcgatgt ccgagatcac cggcggcgaa
      301 ctcttcgcaa gatgcctggc cagcgaagga atcgagcatg tcttcggcct gccttcgccc
      361 gagatcgatc ccctctttgc ccagcttgag aagtacggga tccggctggt cccggtacgg
      421 catgaatcgg ccggcgtcca catggccgag ggcctctaca agtcgaccgg aaaggcttcc
      481 tgtgtgatcg gcaaccccgg cccgggtgcg gccaatctgc tgcccggcat cttcaccgcg
      541 ctgcacgaag gcgttcccgt gctggcgatc acttcgcagc accggatggg agtgagctat
      601 cccacgccgc cttccacctt ccagggccag gaccaactcg attccttcca gcccgctgtc
      661 aaatggggcg gaccgatctt cgaatgggcg cggatacccg aagtgctccg gctcgccttc
      721 cgggagatgt ggaacggccg gcccgggccc gttcacgtcg atgtccccgt caccgtcatg
      781 tatgacctcg gcgaggaaga cgaacagccg atcctcgagc ccgaggccta ccgtccctca
      841 cagccccagg cctctgccgc ccagatcgaa gcggcagcgg acctgctcga gtcggcccgc
      901 agaccggtcg tggtggccgg cgccggtgtt gaccgggcgg gggcgaacgc cgaagcggcc
      961 gaactcgccg aagtgctcgg ctgtccgctc atcccgaccc aggccggccg ggccagcgcc
     1021 cgggccgacc atcccaacta cgtcttcggt ttcggcgaag gcggcatcaa ggccaggctc
     1081 gacgccgacg tgatcctggt cgccggatcc cgcatcggca atctcgacct cccctacgac
     1141 aaatactggg gagagcccgg gacggtgaaa ctgatccaga tcgacaccga cccgagcaac
     1201 atcggtgtga cccgcccgct gaccatgggg atcgtcgccg acgcggccga cgcgctgggc
     1261 gggatcgcgg ccgaactgaa gcggcgtgga aagtccccct cagaagaggc gaccgccgcc
     1321 ctggccgaag cccgcgagac ggatgcccgg tggcatgcct ctcaggcgag taccgccgac
     1381 gaatgggagg gcgaggggat tcaccccgcc cgggcgatcg aagtgatcgg cgagaccttc
     1441 ggcgaccagg cgatctacac ggtggacggc ggcctgaccg cggtctgggc ctatgcgggc
     1501 ctgccgccca ccgggccacg ctcgtaccac agcattctcg aactgggaat gctcgggacc
     1561 ggcatccctt ctgcgatcgg ctcgaagatc ggccagcccg accgggaggt cgtctgcgtc
     1621 accggagacg gagccgccgg gttcaacatc atggaaatgc agtcggccgt ccgggactcg
     1681 atcccggtca ccgtgatcgt ctttgccgag ggcgcctgga ccatggagga gccgaatgag
     1741 ctagccctgt tcggccacac cttcggcacc gagcagggtg aaatccgatg ggacatggtc
     1801 gcggagggac tcggctgcca cggcgagttc gtggatcagc tcgacgacct cggtccggct
     1861 ctgggccggt gccgtgattc cggccagccc tccgtcgtct gcctgaagac agaccgggac
     1921 gcaaaccggg tcatcccccc ggcgatggcc gcccgctgga tggaggtcca ggccggcccg
     1981 atagaggggg tctgagcagg gccgacggcc tacccggagc cgagcgcgcc ccgaagcggg
     2041 ctacccgggg ctcaggcctc ggcagctttg gggccgggga tgttgcggaa gcggaactcc
     2101 tgggggtcca tgccggcctg aacgaaatgg cagacgccgc tctcgggtga gaccatcagg
     2161 tcgacgatcc cgttgaccac atgctcgacc tcgatgatcg ggatgccctc gctgaccagt
     2221 ccttccttga agccatcgat gatccgggtg tccgcgaagc cggggcagat cccgttgata
     2281 cggatgttct ccagctcata gaccgggccg gccgaacgga ccagcccgac cacgccgtgc
     2341 ttgtttgcgg cgtagatcgg gtcgaacgga accgaggtca atccggccag cgaggcggtg
     2401 gcgactatcg cgccgccgcc gcgcttccgc atcgccggca ccgcggcgtt gataccgaag
     2461 acgaccccgt cgaggttgat ctgcatcgcc cggcggtaga gctcgagatc gaaggactcg
     2521 accaggccgc agccggtgga gatgccggcg ttgaggaaag cgatgtccag gccgccgaac
     2581 cggctgaccg caaaatcgac cgctgcttcg ctggcatcgg ggtcgcgcac gtcgcagtgg
     2641 atgaattcgg caccgatctc ggccgccgcc tcccggccgg cttcctcctc gatgtcggcc
     2701 agcacgacat gtgctccttc gccggcaact cgggctccgg tcgcccggcc gaagccgttg
     2761 gcgcccccag tgatcagtac caccttctcc gcaaagctca tgccgggtag catgccatct
     2821 aagggggcag gccggaaatt gcggcttgtc ccgctgttga actaccccgg gagaggtacc
     2881 gagttgaatc cgaatctggc cacactgctg accgagtccg cagagcagtt ccccgagcgg
     2941 accgcaatcc gcctcgaaga caccctgatc ccttatgcgg ggctcgatca gaccagcaag
     3001 ctggtcgcag gactgctcca gtcgagggga gtcgaaaccg gcgaccgggt cggagtcatg
     3061 ctgccgaacg tcccgcactt cccgatggtc tactacgggg tcctgcgcat gggcgcaagg
     3121 gtggttccga tgaacgtgct gctcacagcg cgcgagatca agcattacct tggcaactcg
     3181 ggcgcgaagc tcgtggtcgt ctgggaagac ttcgctgcgg ccgccgtcga ggcagcggaa
     3241 gagctcgggg tcgacgtgat ctcgatcagc ccgaccaacg tcggtgacct gatccagggc
     3301 atcgagccct acgaagatat cgtcgaggtc gagcccgacg acaccgcggt gatcctctac
     3361 acctcgg
//
LOCUS       DAOTIP010000138         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33464_length_3364_cov_3.205198, whole genome shotgun sequence.
ACCESSION   DAOTIP010000138 DAOTIP010000000
VERSION     DAOTIP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33464_length_3364_cov_3.205198"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="P5138_03655"
     CDS             complement(<1..1068)
                     /locus_tag="P5138_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59698.1"
                     /translation="MMVKKSLVTLALGLFAFAALNAQAGAALVPDGYNPKGLPTAGCF
                     WTGPFTASRDLTNIAYPGTEIAYWGAKFRTPAGAVLKLHGKYPHARYSSLNAYESSGG
                     TPTGSISDRQIRPDRGSVNTSLPGKNRKAKKRSWTVRVLGTAAPQNPGRNTLYAESAD
                     DEYQDILYRVYVPDKGRNYAGGTGIPTPELVLQDGTVLKGKALCQAINSNHNYVSNPL
                     PLPLYNSLVNWPGKDPLTNPAPANFSFVKFFNLNLSMARYKTDAEFDATWQANPVEQG
                     TLYANNDARYMTGAVSLRYGKVLVIKGSMPTTPKTWHGNRRTKAGQLAEWDMCSIESL
                     ATTKTHRCLFDQQIPVKGKKRR"
     gene            complement(1170..2459)
                     /locus_tag="P5138_03660"
     CDS             complement(1170..2459)
                     /locus_tag="P5138_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59699.1"
                     /translation="MSGDYVDGVTEPVLSGPELGAAVGVEGVMIRRIARTGVVFLSAT
                     AVLLTCAGAAYAIPWDVGGKVPPSETPAPEFPDPLCQQSYADDLPEPGSRIHFGIGPR
                     LAGEIGTGQATPLVPENWRKRDQALRKLAGSRDFSVRLNRLFMSDGWSGIRRFQKMAR
                     HYGRLGFGVELQVRYHPKPKQDGDIKAWLQYVRNVVRAFGPIRAVKSLQITNEVNLAI
                     SPNTSDGAWKHARRALVSGVKTAQRYSNRLGYGRLKIGFNFAWRFGAQADADFWDDLT
                     RIGGKSLRRATDWIGLDLYPGTYLPPRAAISDYGDAFLEALAQMRECYMPKAGFGSRF
                     PLKIEETGWPTGPGRTEAEQKEILRQFVTTAHLYRGTYGITDFRWFGLRDNNSAGEDY
                     QSYFGLLRDDYSPKPAFGEYRRLLARYGAGKKANDNR"
     gene            2500..>3364
                     /gene="dinB"
                     /locus_tag="P5138_03665"
     CDS             2500..>3364
                     /gene="dinB"
                     /locus_tag="P5138_03665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRV59700.1"
                     /translation="MPVASILHADLDAFYASVEQRDDPSLSGRPVIVGTGVVLAASYE
                     AKARGIYTTMNGRDAHRICPEAVVVAPRMDAYSKASKAVYEIFNDTAPVVEGISIDEA
                     FLDVTGMEHIAGTPVEIAAALRQRVLAEAGLPISVGVARTKFLAKVASGASKPDGLLV
                     VEPDRERAFLHPLPIQALWGVGKVTTARLNGIGIRTVGEVARTSQVRLAAEVGESSAR
                     HLLDLANGRDPRPVEPRERRKSIGAQSAFPRGSRDPAQVESLASALVDRTARRLREAG
                     RACRTVSIGLRF"
BASE COUNT          558 a         1089 c         1095 g          622 t
ORIGIN      
        1 ccggcgtttc ttgcccttga cgggtatctg ctggtcaaac agacagcgat gggtcttcgt
       61 cgtggccaga gactcgatag agcacatgtc ccactcggcc agctggcccg cctttgtgcg
      121 gcggttgccg tgccaggtct tcggcgtcgt cggcatgctg cccttgatta ccaggacctt
      181 cccgtagcga agggagacgg caccggtcat gtaccgggcg tcattgttcg cgtaaagcgt
      241 tccctgctcg accgggttcg cctgccaggt cgcatcgaat tcggcgtcgg tcttgtagcg
      301 ggccatcgag aggttcaggt tgaagaattt gacgaagctg aaattcgccg gcgcggggtt
      361 ggtcagcgga tccttgcccg gccagttgac cagactgtta tagagaggca ggggcagcgg
      421 attcgagacg taattgtgat tcgagttgat cgcctggcag agcgccttgc ccttgagcac
      481 tgtgccgtcc tggaggacca gctcaggggt gggaatgccg gtcccgccgg cgtagttgcg
      541 ccccttgtcc gggacgtaga cccggtagag gatgtcctgg tactcgtcat cggcggattc
      601 cgcgtacagc gtgtttcggc cggggttctg cggcgccgcg gtgccgagga cacgcaccgt
      661 ccaggagcgc ttcttcgcct tgcggttctt gccgggaagg ctggtattga ccgaacccct
      721 gtcgggcctg atctgacggt ccgagatcga accggtcgga gtgccgccac ttgattcgta
      781 ggcgttcagg gacgagtagc gggcgtgggg atacttgccg tggagtttca gcaccgctcc
      841 ggccggggtt cggaacttgg cgccccagta ggcgatttca gttcccgggt aggcgatgtt
      901 ggtgaggtcc ctggacgccg tgaacggtcc ggtccagaaa cagccggccg tcggcagtcc
      961 cttcgggttg tatccgtcgg gcaccagcgc tgcgccggcc tgcgcgttca gggcggcaaa
     1021 tgcgaaaagg cccagggcca gggtcaccaa cgacttcttc accatcatca tgcttcctct
     1081 cctgcccctc ttctggccgc ggactcgcag ccggtcagct cgggatcctc gggatcagtt
     1141 tactgacgcg cgtcaagttt gtggcccatc taccgattgt cgtttgcttt cttccccgct
     1201 ccgtaccggg ccagcagcct ccggtactcc ccgaaggccg gcttcgggct gtagtcatcg
     1261 cggagcagtc cgaaatatga ctggtaatcc tccccggcgc tgttgttgtc ccgcagtccg
     1321 aaccagcgga aatcggtgat gccgtaagtc ccgcgataga gatgggccgt ggtcacgaac
     1381 tggcgcagga tctccttctg ctcggcctcc gtccggccgg gcccggtcgg ccatccggtc
     1441 tcctcaatct tcagcggaaa gcgggagccg aatccggctt tcggcatgta gcactcccgc
     1501 atctgggcga gagcttcgag gaaggcgtcg ccgtaatcgc tgatcgccgc ccgaggcggg
     1561 agatacgtgc cggggtaaag gtcgagcccg atccagtcgg tcgcccgcct caggctcttt
     1621 ccgccgatcc gggtcaggtc atcccagaaa tcggcatcgg cctgtgcgcc gaatcgccag
     1681 gcgaaattga agccgatctt caaccggccg tagccgagcc ggttcgagta cctctgcgcg
     1741 gtcttgactc cgctgaccag ggcccggcgg gcgtgtttcc acgccccgtc ggaagtattc
     1801 ggagagatgg ccagattgac ctcgttggtg atctgcaggg acttcaccgc gcggatcgga
     1861 ccgaaggcgc ggaccacgtt cctgacgtac tgaagccagg ccttgatgtc tccgtcctgc
     1921 ttcggtttcg ggtggtaccg gacctggagt tcaaccccga acccgaggcg tccgtagtgc
     1981 cgggccatct tctggaacct gcggatcccc gaccagccgt cggacatgaa gagcctgttc
     2041 agccggaccg agaagtcgcg gcttccggcc agcttcctca gcgcctggtc gcgcttcctc
     2101 cagttctccg gaaccagcgg agtggcctgc cccgtcccga tctccccggc aagccgcggg
     2161 ccgatcccga agtggatccg ggagccgggc tcgggaaggt cgtccgcgta gctctgctgg
     2221 cagagaggat ccggaaactc gggggcaggg gtctcggacg gcgggacctt tccgccgacg
     2281 tcccacggga tggcataggc ggccccggcg caggtcagca gcaccgcagt agcgctgaga
     2341 aaaaccactc ccgtcctggc aattctccgg atcatcaccc cttcaacacc gacggcggcc
     2401 ccaagctccg gccccgacag aaccggttcc gttacgccgt caacatagtc accgctcaag
     2461 cgacaaagtg acgaccagcg agcctgcgaa catgggttcg tgcctgtcgc ctcgatcctc
     2521 cacgccgacc tcgacgcgtt ctacgcctcg gtggagcaac gagatgatcc ctccctgagc
     2581 ggcaggccgg tgattgtcgg cactggcgtg gtgctggccg ccagttacga agcaaaggcc
     2641 cggggcatct acacgaccat gaacggccgc gacgcacatc ggatctgccc cgaggcggtc
     2701 gtcgttgcgc cccggatgga cgcctattcg aaggcgagca aggcggtcta cgaaatattc
     2761 aatgacacgg cacccgtggt cgagggcatc tcgatcgacg aggccttcct cgatgtgacc
     2821 gggatggagc acatcgccgg cacaccagtc gaaattgcag ccgcgctgag gcaacgagtg
     2881 ctggcggaag ccgggctgcc gatttcagtt ggcgtggccc ggaccaagtt cctcgccaag
     2941 gtggcaagcg gcgcctccaa gccggacggc ctgcttgtgg tggagcccga ccgcgagcgg
     3001 gccttcctcc accctctgcc gatccaggct ctctggggcg tcggcaaggt aaccacggcg
     3061 agactcaacg ggatcgggat ccgaacggtc ggggaggttg cgcgaaccag ccaggtcaga
     3121 ctggccgccg aagtcggcga atcgtcggcc cggcatctgc tcgacctggc caacggccgg
     3181 gacccgcgcc cggtcgaacc ccgggagcga cgtaagtcga tcggagccca gagcgcattt
     3241 ccccggggct cgcgcgatcc cgcccaggtc gaatccctgg cctccgccct ggtcgaccgc
     3301 acagccagaa ggctccgcga agccggaagg gcctgccgga ccgtttcgat cgggcttcgt
     3361 ttcg
//
LOCUS       DAOTIP010000139         3361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33494_length_3361_cov_2.966122, whole genome shotgun sequence.
ACCESSION   DAOTIP010000139 DAOTIP010000000
VERSION     DAOTIP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33494_length_3361_cov_2.966122"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="P5138_03670"
     CDS             <1..472
                     /locus_tag="P5138_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59701.1"
                     /translation="GNVRPLRFIQAMRLPNGSPPRMDLYGHNAYSPRYPKLSRRPAAF
                     GTADLSDFDSLAHWLDRNLARPGRNQRLKIFVSEYSLPTSRNEVIPFWGNRRDQARYA
                     RAALRLVRNFGRAYSLGWFQLFDQPPQANNLQADWGLLDWRNRPKPAFWAFKRG"
     gene            499..1659
                     /locus_tag="P5138_03675"
     CDS             499..1659
                     /locus_tag="P5138_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59702.1"
                     /translation="MGTKRGQIQIACLLGSLILFSGLALVLKGPAPTSTEPALAKAAP
                     DARASAGQRLGKAMWGPAEVNGVSQFPTYKDLGVGIYQMAIDWSQIAATRPATATDPA
                     DPAYQWPEHFEKTILEANRYGMQVTMMVIGAPAWANGGHSDRAWTPKNPKDYADFVTA
                     MSRKYPRVRFWMIWGEPNRSPNFKPLTPSRTGPLNAAQARAPRNYARILDAAYVALKN
                     VDSRDLVIGGNTFTAAGRDSISTYQWIRYMRLPNGGRPRMDLYGHNPFGFRIPSLANK
                     PSPRGMVDFSDSKRLTKVLDRYYPRKRFRLFLSEWGVPAGATEDTELGYKLNYATQAR
                     WIKAGLRLSRKFKRIYTIGWIHPYDRPELGIITGLLKADGRKKPGYFSFRNG"
     gene            complement(1691..2452)
                     /locus_tag="P5138_03680"
     CDS             complement(1691..2452)
                     /locus_tag="P5138_03680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59703.1"
                     /translation="MEDLYLSGYDLAGYFEAFESRKSVLNERRLDRLPRPDGGKTLLD
                     VGCADGQFAAAAALRGWSPSGIELNPPAAEKARARGVEVYQGDIGEIDLSSSRFDLVT
                     SWDVIEHVPNPRLFLEKLASFVKPGGTVVVTTLNRRSLVSRVFRGGWSMIVEDHYTYW
                     DRPSLTRAFAAAGMSVSETRSYGVGRDFVSKIDRWRGSGDNKADPEGAAPATRSDSAR
                     WDVNPVVLAAENGVNLLLDKLSLGVEIEIVSRAPR"
     gene            2793..>3361
                     /locus_tag="P5138_03685"
     CDS             2793..>3361
                     /locus_tag="P5138_03685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRV59704.1"
                     /translation="MTDGLQISAAFATSLGLTWGLVPSAIRLASRTDFFDRPGGYKEH
                     SRSTPYLGGLAVITGFLAASLIFSGADESILAILACVLVLLVVGTIDDRIGLGIVPRI
                     VVEIGVALAVWHFGTGWNLFELEFLNLLFTVAWVVGLINAFNLMDNLDGATSTVADAS
                     ALGAGGLALVEGNSVLAVVGFAMAGACCG"
BASE COUNT          656 a         1049 c         1006 g          650 t
ORIGIN      
        1 aggtaacgtc cggcccttgc ggttcattca ggcgatgcgt ctgccgaacg gcagtcctcc
       61 gcgcatggat ctgtacgggc acaatgcgta ctcgccgcgg tacccgaaac tctcgcggcg
      121 accggccgct ttcggaaccg cggacctttc cgatttcgac tcgctggctc attggctgga
      181 tcggaatctt gctcgtcccg gccggaacca gcgcctgaag attttcgtct ccgagtactc
      241 gctgccgacg agccgtaacg aggtgatccc gttctgggga aatcgccgcg accaggcccg
      301 ttacgcccgg gcggcgcttc gacttgttcg caacttcggg cgggcatact ccctgggttg
      361 gttccagttg tttgaccaac cgccgcaagc caataacctg caggccgatt ggggattgct
      421 cgactggcga aaccggccga agccggcttt ctgggccttc aagcgaggtt gaacgtgaaa
      481 cgaagggaat tggctaaaat ggggacgaag cgcgggcaaa ttcagatcgc ctgcctgttg
      541 ggttccctga tccttttttc cggtctggct ctcgtgctca agggcccggc gccaacttcg
      601 accgagcctg cattggcgaa ggccgctccc gatgcgaggg cttctgccgg tcagcgtctg
      661 ggaaaggcga tgtggggacc ggccgaagtc aatggcgtat cccagttccc cacctacaag
      721 gacctgggcg tcggcatcta ccagatggcg atcgactgga gccagatcgc ggcaacccgg
      781 ccagccacgg cgactgatcc ggccgaccct gcctaccagt ggcccgagca tttcgaaaag
      841 acgatcctcg aagccaaccg ctacgggatg caggtgacga tgatggtcat cggggcgccg
      901 gcctgggcca acggtggtca cagcgaccgg gcctggacgc cgaagaaccc gaaggattac
      961 gcggacttcg taaccgcgat gtcgcgcaag tacccaaggg tccgattctg gatgatctgg
     1021 ggagagccga accggtcgcc caacttcaaa cccttgacac cttcccggac cggaccgctg
     1081 aacgcggctc aagctcgagc gccaaggaac tacgcccgca tccttgacgc ggcctatgtc
     1141 gccctgaaga atgtcgattc aagagacctg gtgatcggag gaaacacttt caccgcggcc
     1201 ggcagggaca gcatctcaac ctatcaatgg atcaggtaca tgcgcttgcc caacggcggc
     1261 aggccccgga tggatctcta cggacacaat cccttcggct tccggatccc aagtctggcg
     1321 aacaagcctt cccccagggg catggtcgat ttctccgatt cgaagcgctt gaccaaggtg
     1381 ctggaccgct actacccgcg caagcgtttc cggctgttcc tctcggaatg gggagtcccc
     1441 gccggtgcga ccgaggatac cgagttggga tacaagctca actatgcgac tcaggcaagg
     1501 tggatcaagg ccgggctccg tctgagccgg aaattcaagc ggatctacac gatcggctgg
     1561 atccatccct atgaccggcc cgagctggga atcatcaccg gactcctgaa ggccgacggc
     1621 agaaagaagc ccggctactt ctcattccgc aacggctagg aacgaaccgg gaaagcccgg
     1681 ttcgttcctt tcatcgaggt gcccgggaga cgatctcgat ctcaacccct agcgaaagct
     1741 tgtccaggag cagattgacc ccattctcgg cggccagaac caccgggttt acgtcccacc
     1801 gcgcagaatc ggacctggtt gcgggcgcgg caccctccgg gtccgccttg ttatcccctg
     1861 acccccgcca tcgatcaatc tttgacacga agtctcggcc caccccgtag ctccttgtct
     1921 cagagacgct catgcccgca gccgcaaatg cccttgtcaa tgaaggtctg tcccaatagg
     1981 tgtagtgatc ttcaacgatc atcgaccagc ccccgcgaaa gacgcgggag acgagcgagc
     2041 gacgattgag ggtggtcacg acgactgtcc cgccaggttt cacaaacgaa gccagctttt
     2101 cgaggaacag gcgagggttt ggcacgtgct cgattacgtc ccatgaagtg accagatcga
     2161 accgggagct actcaggtcg atctccccga tgtcgccctg gtagacctca acccctcttg
     2221 cccgagcctt ttcggcggca ggagggttca actcgattcc cgaaggagac catcctcgta
     2281 acgccgcagc agctgcgaac tgtccgtctg cgcatccgac gtcaagcaac gtcttgcccc
     2341 cgtcgggccg gggcaaacga tccagccgac gttcgttcag cacgctcttt cgggactcga
     2401 acgcctcgaa ataaccggcg aggtcgtaac ccgagaggta gagatcttcc acctgttcga
     2461 tcggctgagg gtcgctatag cccaatccgc aatcacggca aagccagagt tgaagccggt
     2521 catgccgatg ccaacgcttg cgtgcagatc cgccgcacga cgggcattca ccaagaacca
     2581 tggtccggac cctattcggc gggaggcagg gatgtcgccg ggtgtcgccc ggggcggggc
     2641 gccgaaatac caatcgagtt gaggctgatt acgctccggg aggcaggtag tgcgacaagt
     2701 ggcttcaacc accgggaaca tagaatgcat gttgttacgt cgatcgttga atcgggcaca
     2761 ggacgaagtg agaaccaatc gaatggactc gtttgactga cggactccag atatccgccg
     2821 cttttgccac cagcctggga ctgacctggg gcctggtgcc gtcggcgata cgcctggcct
     2881 cgcgcaccga ctttttcgat cggccaggcg gctacaagga gcattcacgt tcgaccccat
     2941 atctcggggg gctcgccgtg atcaccgggt ttctggccgc ttcactcatt ttttccgggg
     3001 cggacgagag catcctcgcg atactggcct gtgtgctggt cttgctggtc gtgggcacga
     3061 tcgacgaccg gatcgggctc gggatcgttc cccgaatcgt ggtcgagatc ggagtcgcac
     3121 tcgctgtctg gcatttcggc accggctgga acctgttcga actcgaattc ctcaacctgc
     3181 tcttcaccgt ggcctgggta gttggcctga tcaatgcctt caacctgatg gacaacctgg
     3241 atggggcgac ctccacggtt gccgatgcca gtgctctggg tgccggtggt ctggctttgg
     3301 tcgaaggcaa ctcggtactg gcggttgtcg gcttcgccat ggctggcgcc tgctgcggct
     3361 t
//
LOCUS       DAOTIP010000140         3355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33574_length_3355_cov_3.547576, whole genome shotgun sequence.
ACCESSION   DAOTIP010000140 DAOTIP010000000
VERSION     DAOTIP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33574_length_3355_cov_3.547576"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="P5138_03690"
     CDS             complement(<1..1712)
                     /locus_tag="P5138_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRV59705.1"
                     /translation="MFEAFSLPFFQRGLAEILLLAIPCGLIGSWVVLRGLAFHSHAVG
                     TATFPGLVLADGLGFAAALGAFGAAALFTLLTVAIARSRRTGADSVTALALAACLAGG
                     VILASDVFNSGANVDTLLFGSLLAIGSGDLWLAAVVAGLAVVASFFVGQHWLARGFDE
                     GASRALRSDSALFDAALLTVVAATVVATLSAVGALLVSTLIVVPAATARLFTARLSTL
                     LTAATLLVAAEGVFGLWLSVQTNAPPGATIAVTSGAAFALALGARTISRRGMWRAAAV
                     AAALLALAGFGAGCGSQSSGGDGPKVVATTTQVGDFVAEIGGDQVDLSTILKPNTDPH
                     EYEPRPSDVQAVADADIIFRSGGHLDDWTEDLIKDSGSDAEVVDLSTHLPVRLFGEEE
                     HDHEGEAHEDEGHEAGGEEFDPHWWQDPVNVRFAAARIESTLSDANPAASAYYQGRLR
                     RFDNQIRNLTRQTKSCVRRVPAGDRKIVTDHDAFAYFTNRFGIETVGTVIPALTTEAQ
                     PSAGDLADLEATIRRENVKAIFPEESVSPALSEALAKDTGATTEYSLYGDTLGPAGSD
                     GETWL"
     gene            complement(1880..3115)
                     /locus_tag="P5138_03695"
     CDS             complement(1880..3115)
                     /locus_tag="P5138_03695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV59706.1"
                     /translation="MKTRRSKPLSLTAFAAVLACLVPAVIGSGTASGAIGTGGGLVIP
                     ETPKISDVICLTGCTKVREVAIGGSVQITGSNMDSVAYVAFRGGKTNIRVKPDLTTRT
                     RVEATVPKGATSGRVRVVSNTNSVSDPSSQVLTVGDKAFSRTGKLTLTDASTAPRKAY
                     QYGLRRPVLNFVVNGSTPRVNLRVDIVNSSGDVISSRFLNNVVTGSTQKVGWSGLVAK
                     RRTAPNGAYRFVVRSADGTAASLSTRLKRQRLKAARAAKTRRGNAADPFGFRMYGFMF
                     PVRGPHTYGDGIGAARSGHTHQGQDVMAKCGTPLRAARAGVVYYNDYQAGGAGNYIVI
                     NTTGTGGKSHAYMHMPARSPLKVGTRVKTGQLIGRVGTTGSSTACHLHFEIWSGPGWY
                     QGGTFLNPTPSLKAWDRYS"
     regulatory      complement(3111..3271)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          572 a         1034 c         1202 g          547 t
ORIGIN      
        1 agccaggtct cgccgtcgga cccggccgga ccgagggtgt cgccgtagag cgagtactcg
       61 gtggtcgccc cggtgtcctt cgccagggcc tcggagaggg cgggggagac cgactcctcg
      121 gggaagatgg ccttcacgtt ctcgcggcgg atcgtcgctt ccagatccgc gaggtcgccg
      181 gccgaaggct gtgcctcggt ggtcagggcg gggatcacgg tcccgaccgt ctcgatcccg
      241 aagcggttcg tgaaataggc gaaggcgtcg tgatcggtga cgatcttccg gtcgccagcc
      301 gggacccgac ggacgcagga cttcgtctgc cgggtcaggt tccggatctg gttgtcgaag
      361 cggcgcagac gaccctggta ataggcggaa gcggcgggat tcgcatcgga gagagtcgat
      421 tcgattctgg ccgctgcgaa gcggacgttg acggggtcct gccaccagtg ggggtcgaac
      481 tcttcgcccc cggcttcgtg gccctcgtct tcgtgagctt cgccttcgtg gtcgtgttcc
      541 tcttcgccaa agagccggac cggcaggtgg gtgctcagat cgaccacttc ggcatcggag
      601 ccgctgtcct tgatcaggtc ttcggtccag tcgtcgaggt gaccaccact gcggaagatg
      661 atgtcggcat cggccacggc ctggacatcg gagggccggg gctcgtactc atgcggatcg
      721 gtgttgggtt tgagaatggt ggagaggtcg acttggtcgc cgccgatctc ggccacgaaa
      781 tcgccgacct gggtggtggt cgcgaccacc ttcggtccgt ccccaccgga gctctggctg
      841 ccgcagcccg ctccgaagcc ggccagggcg agcaacgctg ccgcgaccgc agcggcccgc
      901 cacattcccc ttcgggaaat tgtccgggcg ccgagggcaa gggcgaatgc ggccccgctg
      961 gtcacggcga tcgtcgcgcc gggaggagcg ttggtctgga cggaaagcca gagcccgaaa
     1021 actccttcag ctgcgaccag gagggtcgcg gcggtcagga gggtggagag ccgcgccgtg
     1081 aatagccggg cggtggctgc cggcacgacg atcagggtgg aaaccaggag agcacccacc
     1141 gcactcaggg tggccaccac ggtcgctgcg accacggtca agagggcagc gtcgaacagg
     1201 gccgagtccg agcggagcgc tctcgaggcg ccttcgtcga atcccctggc gagccagtgc
     1261 tggccgacga agaacgaggc gaccaccgcc agtccggcga cgacggccgc cagccagagg
     1321 tctcccgaac cgatcgccag cagcgagccg aacagcaggg tatccacgtt ggcccccgag
     1381 ttgaagacgt cacttgcgag gatcacgccg ccggccagac atgcagccag ggccagcgca
     1441 gtgacgctgt cggcgccggt ccggcgggac cgggcaatcg ccacggtgag cagggtgaac
     1501 agggcggcgg ctccgaatgc gccgagcgcc gccgcgaaac cgagcccgtc ggcaaggacc
     1561 agtccgggga aggtcgcggt gccgaccgca tgcgaatgaa acgcgaggcc gcgcagcacg
     1621 acccacgagc cgatcaggcc acaggggatg gcgagaagga ggatttcggc caggccccgc
     1681 tggaaaaagg ggagtgagaa cgcttcgaac aattcggctc cggatcgggt aactgaagaa
     1741 tgcagagaat gagattcgtt ctcattacta ccggagcata cagaattact gagaacaagt
     1801 ctcaataagg cggaccaagc cctcggcggg gcggagccag ggccggattc cggcgccgct
     1861 cagccggcgg ggaggcggat cagctgtagc ggtcccaggc cttgagggaa ggagtgggat
     1921 tgaggaaggt gccgccctgg taccagccgg ggccggacca gatctcgaaa tggaggtgac
     1981 aggcggtgct tgaaccagtg gtgccgaccc ggccgatcaa ctggccggtc ttcaccctgg
     2041 tgccgacctt cagcggcgag cgggccggca tatgcatgta ggcgtggctc ttgccgccgg
     2101 tgccggtcgt gttgatcacg atgtagttgc cggccccacc cgcctggtag tcgttgtagt
     2161 agacgacgcc ggcccgggca gcccggagcg gggtgccgca cttggccatg acgtcctggc
     2221 cctgatgggt gtgtcccgac ctggcggcgc cgatgccgtc cccgtaggtg tgggggccgc
     2281 gcaccgggaa catgaatccg tacatgcgga atccgaaggg atcggcggca ttcccgcgac
     2341 gggtcttggc ggcccgggcg gccttcaggc gctggcgctt caaccgggtc gagagtgagg
     2401 ccgcggtgcc gtctgcgctc cggaccacga agcggtaggc gccattgggg gcggttcggc
     2461 gcttggcgac gaggccgctc cagccgacct tctgggtcga tccggtgacg acgttgttga
     2521 ggaaccggct gctgatcacg tcaccgctcg agttcacgat gtccacccgc aggttgaccc
     2581 tgggcgtgga accgttgacc acgaaattga gtaccggcct gcggaggccg tactggtaag
     2641 ccttgcgggg agcggtgctg gcgtcggtga gggtgagctt cccggtccgg gagaacgcct
     2701 tgtcgcccac ggtcaggacc tggctcgagg gatcggaaac agagttcgtg ttggagacga
     2761 cccggacccg gccggaggtc gcgcctttgg gcaccgtcgc ctcgaccctg gtccgcgtgg
     2821 tcaggtccgg cttcacccgg atgttcgtct ttccgccgcg gaaggcgacg tatgcgaccg
     2881 aatccatgtt tgacccggtg atctgtaccg agccaccgat cgcgacttcg cgcaccttcg
     2941 tgcagccggt caggcagatc acgtcggaga tcttcggggt ctcgggaatg accagtccgc
     3001 cgcctgtgcc gatcgcgccg ctggccgtgc ccgagccgat cacggccgga accaggcagg
     3061 cgaggaccgc cgcaaaggcg gtaagtgaga ggggctttga tcgtctcgtc ttcaactctt
     3121 tccagatgcc tgcggagtta gctggcgggc tcgcgctgaa agagttgcgc tacggagcaa
     3181 cgactgcctc cgattcgccc cttggggaaa ggcttccccg ttggttcctc cgccccttcc
     3241 ccgtaaggaa agggttcggc tttttgacgc gcgtcagtat aagcggatcg aaagatgttc
     3301 gtgcggccgc cggtgaagac cggatcactt gcagatgaca gcggtccgac tgcag
//
LOCUS       DAOTIP010000141         3355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33578_length_3355_cov_3.221515, whole genome shotgun sequence.
ACCESSION   DAOTIP010000141 DAOTIP010000000
VERSION     DAOTIP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33578_length_3355_cov_3.221515"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="P5138_03700"
     CDS             <1..1460
                     /locus_tag="P5138_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59707.1"
                     /translation="NLVTDISPGLASSDPGVSAELNGMIYFAASDPDHGRELWRSDGT
                     SAELVKDIYPGPEDSFPQNLVTVGNRVFFSAADSVNGRELWQVDAGGASLVYDINPGL
                     PSSSPAFLTSFTGKVFFQAYTPTHGQELWSSDGVTTGEVADLYPGGGSSQVSQLAVAG
                     GNLYLAAYLPATGIELYKSTGGVPTLVSDVEPGASSSYPDSLTAFGDKVVFRAHTMAT
                     GDEPWISNGTGATQIADISPGADASYPGNFTILDGTVYFTASTPDLGVEVYRSDGVTA
                     DLAGDPIPGKFGGAYGLKGIDGNLYFAASTPEYGSEPWILDGSGSRMIRDLLPGPEGS
                     YPTGFAAHGDGVLFGADDGTHGLEPWFMHPDREVKLALLGKRLGVSKKGFAGLKLACP
                     ANEANGPCISKITVATRKPVRVKGKRRKVTLSRKSIRVLTGNTGTARLNFSRANIRLL
                     LKQPAARRVLVTVKVRDKAGNKRTVRKRYLLGRPA"
     gene            complement(1457..2545)
                     /gene="proB"
                     /locus_tag="P5138_03705"
     CDS             complement(1457..2545)
                     /gene="proB"
                     /locus_tag="P5138_03705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRV59708.1"
                     /translation="MTLVVKLGSSIVSADDGSLREEVLDSVCSQAAALRRAGEPLVMV
                     TSGSIARGIRLLGLQARPRAIDELQAASAVGQGSLFREYETRLEAGETAAAQVLLTAS
                     DVANRTSYVNARQTLRRLLDWGVVPVINENDTTATDEITFGDNDFLAAQVAIMLEARL
                     LVLLTNTDGVYTADPARDAEASLIEEVSDPGDLESMEIGDRTSVFGTGGMRSKVAAAG
                     MASGSGIEVRICNGTVDGTLARAAAGESAGTAFPALRSDAPAFKQWLRYAKPVAGRIV
                     VDDGAARVLKQSGSSLLPVGVTAVEGSFEAGDAVEVVAGGSVIGKGIANYSAGETERI
                     KGMKSAEVLELMPHAAEELIHRDYFVLA"
     gene            complement(2568..>3355)
                     /gene="cgtA"
                     /locus_tag="P5138_03710"
     CDS             complement(2568..>3355)
                     /gene="cgtA"
                     /locus_tag="P5138_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HRV59709.1"
                     /translation="SLLARLTRADPKVADYPFTTLEPVLGTIETPDRQVVLADIPGLI
                     EGAAEGAGLGHEFLAHVERCAMLIHVVELAPSEGEPLANYRAVRAELAAHSTGLEDLP
                     ELLVLSKSDLWTKADMADQVEEWDRELGDSVLGIIPVSSATGAGLERLKEAIVRFVPD
                     HVEAPVVTSADGVEVDFEAEHLTYRPKGEGGYSVEREDDGAFRIHGHGIEVLFERFDT
                     NNEEALAYLETRLTEIGVMAELKRAGFESGDEIRVGELEFELY"
BASE COUNT          575 a         1141 c         1068 g          571 t
ORIGIN      
        1 tcaaccttgt caccgacatc tctcccgggc tcgcgtcatc cgatcccgga gtcagcgccg
       61 aactgaacgg gatgatctac ttcgccgcca gcgaccccga ccacggcagg gagctgtggc
      121 gctccgacgg aacttccgcc gagctcgtca aggacatcta ccccgggccg gaggattcct
      181 tcccgcagaa tctcgtgacg gtcggcaacc gggtgttctt ttccgctgcc gactccgtca
      241 acggacggga gctgtggcag gttgatgccg gtggagcttc cctggtctac gacataaacc
      301 ccggcctgcc ctcgtcttca cctgccttcc tgacctcctt cacgggcaag gtgttctttc
      361 aggcgtacac gccgacccat gggcaggagc tctggtccag cgacggcgtc acgaccggcg
      421 aagtagcaga cctctacccg ggcggcggca gctcacaggt ttcccagctg gccgtagcgg
      481 gaggaaacct gtatctggcg gcctatctcc cggcaaccgg aatcgagctc tacaagagca
      541 ccggcggagt tccaactctg gtcagcgacg tcgagcccgg ggcgagttcc tcctacccgg
      601 acagtctgac cgccttcggc gacaaggtgg tgttccgggc acacaccatg gccaccgggg
      661 atgagccatg gatcagcaac ggaaccggag cgacccagat tgccgacatc agtcccggag
      721 ccgatgcttc ctacccgggc aatttcacga ttctcgacgg gacggtctac ttcaccgcct
      781 ctacccccga cctcggggtt gaggtctacc ggtcggacgg cgtcacggcc gacctggcgg
      841 gcgacccaat tcccggcaag ttcggcggcg catacgggct gaaagggata gacggcaacc
      901 tgtacttcgc ggccagcacc ccggaatacg gctccgaacc atggatcctc gacgggtccg
      961 ggagccgcat gatccgggac ctgctgcccg gccccgaggg gtcctacccc accggcttcg
     1021 ctgctcacgg ggatggcgtg ctctttggcg ccgacgacgg cacccatgga ctcgagccct
     1081 ggttcatgca tcccgatcgg gaggtcaagc tcgccctgct cgggaaaaga ctcggagtct
     1141 cgaagaaggg ctttgccggg ctgaagctgg cctgtccggc caatgaggcg aatggcccgt
     1201 gcatttcgaa gatcaccgta gcgacgcgga agccggtccg ggtcaagggc aagcgccgca
     1261 aggtgacgct ctcgcgcaag tcgatccggg tgctgaccgg aaacaccggc accgcccggc
     1321 tgaacttcag ccgggcgaac atccggctgc tactgaagca acccgcggcc cgccgggtgc
     1381 ttgtcacggt caaggttcgg gacaaggccg ggaacaaacg gacggtcagg aagcgctacc
     1441 tgctgggcag gcccgcctag gccagcacga agtaatcgcg gtgaatcagc tcttcggccg
     1501 catggggcat gagttcgaga acctcggcgc tcttcatgcc cttgatccgt tctgtttcgc
     1561 ccgccgagta gttggcgatg cccttgccga taaccgagcc gcccgccacg acctcgaccg
     1621 cgtcgcccgc ctcgaatgag ccttccaccg cggtcacccc caccggcagg agactggagc
     1681 cgctctgctt cagcacccgg gcagcgccgt catcgacgac gattcgcccg gccaccggct
     1741 tggcgtatcg cagccactgc ttgaaggccg gggcatcgct gcgcagggcc gggaaggcgg
     1801 tcccggcgga ttcaccggcc gccgccctgg ccagggtgcc gtcgaccgtc ccgttgcaga
     1861 tccgcacttc gattcccgat ccgctggcca tgccggccgc ggccaccttg cttcgcatcc
     1921 cgccggtccc gaagaccgag gtgcggtcgc cgatctccat cgactccagg tcgcccgggt
     1981 cggatacttc ttcgatcagg ctcgcttccg cgtcccgtgc cggatcggcg gtgtagacgc
     2041 catcggtgtt ggtcaggagg acgagcagcc gcgcttccag catgatcgcc acctgggcgg
     2101 ccaggaagtc gttgtccccg aaggtgatct cgtcggtcgc ggtggtgtcg ttctcgttga
     2161 tgaccggaac gacgccccag tcgagcagac gacgaagagt ctggcgcgcg ttcacatagc
     2221 tggtgcggtt ggcgacgtcc gatgcggtca ggagcacctg ggcggcggca gtctcgcccg
     2281 cctccagccg ggtttcgtat tcacgaaaga gcgagccctg accgacggcg gaggccgcct
     2341 gcagctcgtc gatcgcccgc ggccgggctt gcagtccgag caggcggatg cccctggcga
     2401 tcgaacccga ggtgaccatg accaacggct ccccggcccg cctcagcgcg gccgcctggg
     2461 agcagacaga atcgagcacc tcctcgcgaa ggctcccgtc atcagccgag acgatcgaag
     2521 atccgagttt caccaccagc gtcatagcca gacaagccta ggacttatca gtacagctcg
     2581 aactcgagct caccgacacg gatttcatct cccgattcga aaccggcacg cttcagctcc
     2641 gccatgaccc cgatttcggt cagacgggtt tccaggtatg cgagcgcttc ttcgttgttg
     2701 gtgtcgaagc gttcgaagag aacttcgatg ccgtggccgt ggatgcggaa ggcgccgtcg
     2761 tcttcgcgct cgaccgagta gccgccttca cccttgggcc ggtacgtgag gtgttccgct
     2821 tcgaagtcga cctccacccc gtcggcggag gtgaccaccg gcgcttcaac gtgatccgga
     2881 acgaaacgga cgattgcttc cttgagcctt tcgagccccg cgcccgtggc cgatgacacg
     2941 ggaatgattc cgagcacgga gtcgccgagc tcccggtccc attcctcgac ctgatcggcc
     3001 atgtcggcct tggtccagag atcggacttg gaaaggacca gcagttcggg aaggtcctca
     3061 aggccggtgc tgtgcgcggc cagctcggcg cggaccgccc ggtagttggc cagcggctcg
     3121 ccctccgagg gggccagttc gaccacgtgg atcagcatcg cgcagcgttc gacatgagcc
     3181 agaaactcgt gccccagtcc ggccccttcg gccgccccct cgatcaggcc ggggatgtcg
     3241 gccagaacta cctgacggtc gggcgtctcg atggtgccga ggaccggctc cagggtggtg
     3301 aagggatagt cggcgacctt ggggtcggct cgggtgagcc gggccagcag ggaac
//
LOCUS       DAOTIP010000142         3343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33742_length_3343_cov_3.262165, whole genome shotgun sequence.
ACCESSION   DAOTIP010000142 DAOTIP010000000
VERSION     DAOTIP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33742_length_3343_cov_3.262165"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="P5138_03715"
     CDS             <1..671
                     /locus_tag="P5138_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRV59710.1"
                     /translation="VDELLSPASALACQMDLVSGGEEAVATTLTAIDAAAGAGIGTVN
                     VLTGPNLWEEGVVRSYDEAAWTTALGALERICKRGEDAGVQIGFEPCWGTLAHDAATA
                     QRVLDAVPVSVTFDPSHFVMTGDSIPDLIDRWGERIVHFHLKDAFGRPGMDGEDFIFC
                     MLGEGNVPWLETLEALDRIAYDGPLSVEFEAYRYYEQVLGNDPAAAARLARGQVAALL
                     GEDE"
     gene            668..1672
                     /locus_tag="P5138_03720"
     CDS             668..1672
                     /locus_tag="P5138_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRV59711.1"
                     /translation="MKVALCGLGEIGQIHLKAIRESGHELVAVLELNRRLAVESVGDD
                     VPIHTEVVEMVELTEPDVVDICLPHHLHVPIAVAALEAGADVLLEKPMAMDLEGCDEI
                     IEAADRHGRKVGISHNQVHFRPHRRLRELIDSSQLGEIRAIYERLWMGGKYGGWREDS
                     SQVGGGLLMDAGVHRVYMAEYLGGPVKAVTAVMDEPRSEDAFVLTLEFESGAIGVIQG
                     GYHGPDGVFDDRIDIQGSAGMAEVLGCEAFFEGDLTGDLRLRAQLGGEWSDDPVTGDW
                     DESVTDSVKACLDDLSQGNEPEVGMAEGRDAVAVVEAAYRSAETGKTISISDLDERAS
                     "
     gene            1669..2406
                     /locus_tag="P5138_03725"
     CDS             1669..2406
                     /locus_tag="P5138_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV59712.1"
                     /translation="MSINADTGSKGSLREIKAESSLTARVRESIREAIIDGSLEPGSL
                     HSVKSLADIFKVSRTPVREALIDLAGADMVEFERNRGVRILETSVHDLEEILVLRILL
                     EVPATHRAAERIDADGLSALRQELDAMVEAAENGDEPTMMQHDRRFHELINTASGNSR
                     LTEYVDSLRDLILTRGVSTVDSTRSLPEIVAEHEGILAALASGDADAAATAMKQHLVN
                     TASLLLQQEGGRSTSLSLGWEGLVVGE"
     gene            2430..>3343
                     /locus_tag="P5138_03730"
     CDS             2430..>3343
                     /locus_tag="P5138_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV59713.1"
                     /translation="MSSIRSINPFDPSDLVAETQAASPEGVESVLASGVAAREGWAAD
                     PMLRLRSLGALADEIGLRRQQFVDLMVREVGKPEPEAAGEVDRATSILRFYSQIPLDP
                     VGEVVPGSMPGARVLVERHPLGLVLAICPWNFPLAIPLWKAAPALAYGNTVVVKPASP
                     AIATAALLSECAAEVLPDGVLTVLPMGGARTAEVLDDERIAAATFTGSTGVGISVAER
                     LARRAAPAQAEMGGQNAAIVLDDADLEAAANAIVSGAMGYAGQKCTATRRVVALGSIR
                     EELEAKLAGLVEGLSVGNPADAGVTVGPL"
BASE COUNT          596 a         1025 c         1152 g          570 t
ORIGIN      
        1 acgtcgacga actgctgtcg cccgcctccg cgctcgcctg ccagatggac ctggtcagcg
       61 gcggcgagga agcggtcgcg accacgttaa cggcgattga cgccgcggcc ggggcgggca
      121 tcggcacggt caatgtgctg accggtccca acctctggga ggagggtgtg gtccgcagct
      181 atgacgaagc agcctggacg acggccctgg gggcactcga acggatctgc aagcgtggcg
      241 aggacgccgg ggtgcagatc ggtttcgagc cctgttgggg aaccctcgcc cacgacgcag
      301 ccacggccca gcgggtgctg gacgcggtcc cggtctcggt caccttcgat cccagccatt
      361 tcgtgatgac cggcgattcg attcccgatc tgatcgatcg ctggggggaa cggatcgtcc
      421 acttccacct caaggacgct ttcggacggc ccggcatgga cggggaggac ttcatcttct
      481 gcatgctggg tgagggaaac gtgccctggc tggaaaccct cgaggcgctc gaccggatcg
      541 cctacgacgg cccgctgtcg gtcgaatttg aggcctaccg gtactacgag caggtgctcg
      601 gcaatgatcc ggccgccgcg gcgaggctgg cccgcggcca ggttgccgcc ctgctcgggg
      661 aggacgaatg aaggtcgcgc tctgcgggct cggcgagatc gggcagattc acctgaaggc
      721 gatccgcgag agcggtcacg aacttgtggc tgtcctcgaa ctgaaccggc gccttgctgt
      781 cgagagcgtc ggcgacgatg taccgatcca cacggaggtt gtggagatgg tcgagctgac
      841 cgaaccggat gtggtcgaca tctgcctacc tcatcacctt catgtgccga ttgccgtcgc
      901 agcgctggaa gccggtgctg acgtgctgct tgagaaacca atggcgatgg acctcgaagg
      961 ctgcgacgag atcatcgagg cggcagaccg gcacgggcgc aaggtgggta tatcccacaa
     1021 tcaggtgcac ttccgtcccc atcgccggct tcgcgaattg atcgactcca gccagctcgg
     1081 cgagatcagg gcgatctacg aacgactctg gatgggcggc aagtacggcg gctggcggga
     1141 ggactcaagc caggtaggag gcggtctgct gatggacgcc ggagttcatc gggtgtacat
     1201 ggccgagtac ctcggcggac cggtgaaagc cgtgaccgcg gtcatggacg agccgcgatc
     1261 cgaggatgcc ttcgtcctga ctctcgagtt cgagtccggg gcgatcgggg taatccaggg
     1321 cggctaccac ggtcccgacg gtgtcttcga cgaccgcatc gacatccagg gatcggcagg
     1381 gatggccgag gtgctcggct gtgaggcgtt cttcgagggc gacctgaccg gtgacctccg
     1441 gctcagggca cagctcggcg gggaatggtc cgatgatccg gtgaccggcg actgggatga
     1501 atcggtcacg gattcggtca aggcctgtct cgacgatctg tcgcagggaa acgagcccga
     1561 ggtggggatg gcggaaggcc gtgacgcagt tgccgtggtc gaggctgcct atcgttctgc
     1621 cgagactggc aagacgatca gcatcagcga cctggacgag agggcctcat gagcatcaac
     1681 gccgacactg gaagcaaggg atcgcttcgc gagataaagg ccgaatcgag cctgacggcg
     1741 agggtgcgtg aatcaatccg ggaagcgatc atcgacggca gcctcgagcc cggttcgctc
     1801 cattcggtga aatcgctggc cgacatcttc aaggtttcgc gaacccccgt gcgcgaagcg
     1861 ctgatcgatc tcgctggcgc tgacatggtc gagttcgaaa ggaaccgcgg agtaaggatc
     1921 ctcgagacgt ccgttcacga cctcgaggag attctggtcc tgcggatcct gcttgaggtg
     1981 ccggcgacac accgcgctgc cgagcgcatc gacgccgacg gactgtcggc cctgaggcag
     2041 gaactcgacg cgatggtcga ggccgccgag aacggtgacg aaccgacgat gatgcagcac
     2101 gaccgccgct tccacgaact gatcaacacg gcttccggaa actcgaggct cacggaatat
     2161 gtggactcgc ttcgtgacct gatcctgacc cggggcgttt cgaccgtgga cagcacccgc
     2221 tccctgcccg aaatcgtcgc cgagcacgaa gggatcctcg ccgcactcgc atcgggtgat
     2281 gcggacgcgg cggcaactgc catgaaacag catctcgtca acaccgcctc gctcctcctc
     2341 cagcaggaag gtggccgcag cacttcgctc agcctcggct gggaaggcct ggtggtgggc
     2401 gaatgagcga gggaaactcg gaatcagcca tgtcttcgat caggagcatc aacccgtttg
     2461 atccctcgga ccttgttgcc gagactcagg ctgcgtcgcc ggaaggagtc gagtcggttc
     2521 tggcttccgg cgttgcggcc cgggagggct gggcagcgga cccgatgctc aggctgaggt
     2581 cactcggtgc gctggctgac gagatcggcc tgcgccggca gcagttcgtt gacctgatgg
     2641 tcagggaggt cggcaagccc gaacccgagg cggcggggga agtcgaccgg gcgacctcga
     2701 ttctgaggtt ctactcccag atcccccttg atccggtcgg agaggtcgtg ccgggctcga
     2761 tgcccggggc cagggtgctg gttgaacgac acccgcttgg gctggtcctc gcgatctgcc
     2821 cctggaactt ccccttggca atccccctgt ggaaggcagc tcccgcgctg gcttacggaa
     2881 acaccgtcgt ggtcaagccg gcatcgccgg cgatcgccac ggcggccctg ctctccgaat
     2941 gcgcggccga ggtgcttccc gacggagtcc tgaccgtcct gccgatgggc ggggcccgga
     3001 ccgccgaagt actcgatgac gagcggatcg cggcagcgac cttcaccgga tcgaccggag
     3061 tgggcatttc cgtcgccgag cgtcttgcca ggcgggccgc gcccgcacag gcggagatgg
     3121 gaggtcagaa tgccgccatc gtccttgacg acgccgatct cgaagcggcg gctaacgcaa
     3181 tcgtttcggg tgccatgggt tatgccggac agaaatgcac ggcaacccgc cgcgtcgtcg
     3241 ctcttggttc gatccgggaa gagctcgagg cgaaacttgc cggtctcgtg gaaggacttt
     3301 cggtcggtaa tccggccgat gccggagtga cggtcggccc cct
//
LOCUS       DAOTIP010000143         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_33796_length_3338_cov_3.045081, whole genome shotgun sequence.
ACCESSION   DAOTIP010000143 DAOTIP010000000
VERSION     DAOTIP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33796_length_3338_cov_3.045081"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="P5138_03735"
     CDS             complement(<1..660)
                     /locus_tag="P5138_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRV59714.1"
                     /translation="MASERVHRTKVALLGAAIVVLFVGEYNEQLAIESVEFATLGLLA
                     GMMVLVYLTSQSGIYDFIAVKAGQVSRGNPFVLVVMMAATVTVLAGFLDNLTTILLIV
                     PITFLLADTLDIDPMPLILVEVVSANIGGAATLIGDPPNIMIGNAAGLSFNDFLLNTM
                     PAAVSILAVISVALYFVFRKRIQVAEENKKYVMELDARASIRDWSELKRTLPVLLGTI
                     VF"
     gene            complement(749..1897)
                     /locus_tag="P5138_03740"
     CDS             complement(749..1897)
                     /locus_tag="P5138_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV59715.1"
                     /translation="MFGGGNSITLFRVAGIRIAVDWSWFVFLFIVIVWLSSFYGDILD
                     EPGSTQSYLLAVASAAGFFGSILLHEMGHAFAAKRSGIGIRTIRLWLFGGIAEMDQES
                     PNPGTEFKVAVAGPLVSLLIAIVLCVIGIQAAGVNDFRDALEMRITASTTGPITMVAW
                     LAAVNILVLGFNLLPAFPMDGGRIVRSIAWKVTGKRSAATRFAAGLGRIFGFLFIALG
                     IVWAVNGNVFSGLWLAMIGFLVNSSARAASQSTVTEKLEHVTVAEVMDPEPVAIPGDI
                     DCARAMEEYFLRFGWSWFPVVDASGTFIGMAVRDRVEAVPEVERMTTRVDTLTERDPA
                     FAVSRTAGLDSLLGNPQLRRFGALMVTDDQGLLAGVVTIEQLGRALQA"
     gene            1915..3186
                     /locus_tag="P5138_03745"
     CDS             1915..3186
                     /locus_tag="P5138_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRV59716.1"
                     /translation="MRHGGGAGKWICCAFMSPTAPKRLAAPHGEADQQWLRQKLEELE
                     RIERPSASEGELQAAEWLVGELAKLGAEARIETERAHGTYWWPIGIGTSLGVAGGLAA
                     LRGRRLAGAALGALGAAVIATDFPPRPRPFRKLLPERDTFNVICELGDPDADRTVVLV
                     SHHDSANAGLVFHPGIPDLVSKTGAFRHLDTSPMLMAPVIGGPVTSLLAGLTGSRVLA
                     AAGTVLSAGSTASMIDIGLRKVVPGANDNGTAVIALLELARRFLADPPDDLRLILLSA
                     GSEESFSEGMKAFGERHFDNLPVDSTFFINMDTIGSPYLTVLRGEGFLKMFEYPAEAL
                     ILADNAAEEEGIRLMPNLRIRNGTDGLEALAAGYPVVSICSCTEHKQPANYHWPNDVS
                     ENVDFDTVESGIRLAEGMTRRLAGGWVSLPD"
BASE COUNT          605 a         1079 c         1102 g          552 t
ORIGIN      
        1 gaagacgatc gtgccgagca ggaccggcag ggtgcgcttc agctcactcc agtcccggat
       61 cgacgcacgc gcatccagtt ccatcacgta tttcttgttc tcttcggcaa cctgtattcg
      121 ttttcggaag acgaagtaga gcgcgaccga gatcacggcc aggatcgaaa cggcggcggg
      181 catcgtgttc agcaggaagt cgttgaagct caggccggcc gcgttgccga tcatgatgtt
      241 cggcgggtct ccgatcaggg tcgcggcacc gccgatgttt gccgagacca cctcgaccag
      301 gatcagcggc atcgggtcga tgtccagggt gtcggcgagc aggaaggtga tcgggacgat
      361 cagcaagatc gtcgtcaggt tgtccagaaa gccggccagc acggtgaccg tggcagccat
      421 catcaccacc aggacgaagg gatttccgcg ggaaacctgg ccggccttga ccgcgatgaa
      481 gtcgtagatg ccgctctggc tggtcaggta gaccaggacc atcatcccgg ccagcaggcc
      541 caaggtggcg aactcaaccg actcgatcgc gagttgctcg ttgtactcgc cgacgaacag
      601 caccacgatc gccgccccga gcagggccac cttggtccgg tgaacccttt cgctcgcgat
      661 cagggcgagg acgatcagaa atgtcgcaac agccagtgcc agaggccgag tctatgcact
      721 cgcccggagg gaagcgttca agcccgtttc aggcctggag ggcccggccg agctgctcga
      781 tggtgacgac cccggccagc agaccctggt cgtcggtgac cataagggcg ccgaatcggc
      841 ggagttgggg attgcccagc aggctgtcga gaccggcagt ccggctgacc gcgaaggcgg
      901 gatcccgttc ggtcagggtg tcaacgcgcg tggtcatgcg ctcgacttcg ggtaccgcct
      961 cgacccggtc ccgcacggcc atgccgatga aggtgccgct ggcgtccacc actggaaacc
     1021 atgaccagcc gaaacgtagg aagtactcct ccatcgcccg ggcacagtcg atgtcgcccg
     1081 ggatggccac cggttccggg tccattacct ccgcgaccgt cacgtgttcg agcttctcgg
     1141 tgacagtgct ctggctcgcc gcccgtgcgg agctgttgac caggaagccg atcatcgcca
     1201 gccagaggcc gctgaagacg ttgccgttga cggcccagac gatgccgagg gcgatgaaga
     1261 ggaaaccgaa gattcggccg aggccggccg cgaagcgggt tgccgcgctg cgcttgccgg
     1321 tcaccttcca ggcgatcgac cggacgattc gtccgccgtc catcgggaag gcgggcagca
     1381 ggttgaagcc gagcacgagg atgttcactg ccgcaagcca ggcgaccatg gtgatcggcc
     1441 cggtcgtgga ggcggtgatc cgcatctcca gggcgtctcg gaaatcgttg acaccggcgg
     1501 cctggatccc gatcacgcag agcacgatgg cgatcagcag gctgaccagc ggtcctgcga
     1561 cggcgacctt gaactcggtc ccggggttcg gcgactcctg gtccatctcg gctattccgc
     1621 cgaatagcca gagccggatc gtgcgaatgc cgatcccgct gcgctttgcg gcaaaggcgt
     1681 ggcccatttc gtgaagcagg atggaaccga agaatcctgc cgcactggcg accgcgagca
     1741 ggtaggactg ggtgcttccg ggctcgtcga ggatgtcgcc gtagaagctg gaaagccaga
     1801 cgatcacgat gaacaggaag acgaaccagg accagtccac cgcgatcctg atcccggcca
     1861 cccggaagag cgttatcgag ttgccaccgc cgaacacgtc cacatcgtat ggacatgcgt
     1921 cacgggggcg gtgccggcaa gtggatatgt tgcgccttca tgtcgccgac cgcaccgaaa
     1981 agactggcag cgccgcacgg tgaagccgat cagcagtggc ttcgccagaa gctggaagaa
     2041 ctcgaacgga tcgagcgccc ctcggcttcc gaaggtgaac tgcaggccgc cgaatggctg
     2101 gtcggcgagt tagcgaagct cggagccgaa gcccggatcg aaacggaaag ggcccatggc
     2161 acctactggt ggccgatcgg gatcggcacc tcactcggcg tggccggcgg gctcgccgct
     2221 ctccggggca ggaggctggc cggggctgcg ctcggcgcgc tcggggcggc ggtgatcgcg
     2281 accgacttcc cgccgcgccc gcggcctttc cggaagcttc tgcccgaacg ggacaccttc
     2341 aacgtgatct gcgaactcgg cgatccggat gcggatcgaa ccgtggtact cgtctcccac
     2401 cacgattccg ccaacgccgg gctggtcttc catcccggca tcccggatct ggtgtcgaag
     2461 accggtgcct tccggcatct cgacacctcg ccgatgctga tggcaccggt gatcggcggt
     2521 ccggtcacca gcctgctggc cggcctgacc ggcagtcgcg tcctggcggc cgccggaact
     2581 gtgctctcgg ccggttcaac cgcgtcgatg atcgacatcg gcctgcgcaa ggtcgtgccc
     2641 ggagccaacg acaacggcac tgccgtgatc gccctgctcg aactcgctcg gcgcttcctg
     2701 gccgatccgc cggacgacct ccgcctgatt ctgctctcgg ccggatcgga ggagtccttc
     2761 agcgagggaa tgaaggcctt cggcgagcgt cacttcgaca atctgccggt tgattccacc
     2821 ttcttcatca acatggacac gatcggctcg ccgtacctga cggtactgcg gggcgaaggg
     2881 ttcctgaaga tgttcgagta ccccgccgag gcgctgatcc tcgccgacaa tgcggcggaa
     2941 gaggagggga tcagactgat gcccaacctc aggatcagga acgggaccga tggcctggag
     3001 gcgctggccg ccggctatcc cgtggtgtcg atctgctcat gcaccgagca caagcagccg
     3061 gccaactatc actggccgaa tgacgtttcc gaaaacgtcg acttcgacac ggtcgagtcc
     3121 ggaatcaggc tggccgaggg aatgacccgc cggctcgccg gcggctgggt cagcctgccc
     3181 gactgaccgg cccgccgttg accctgtcgg ggctgcccgc gcgttcgctc aggcctgatc
     3241 ctcggggtca ccccagcgat tgtccgggta gtcgcggtcg cgctcgtccg cgacgggctt
     3301 gccgtccttg gtcgtgatcg acgcgccgac gaggcgac
//
LOCUS       DAOTIP010000144         3315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34061_length_3315_cov_3.008896, whole genome shotgun sequence.
ACCESSION   DAOTIP010000144 DAOTIP010000000
VERSION     DAOTIP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34061_length_3315_cov_3.008896"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="P5138_03750"
     CDS             complement(<1..574)
                     /locus_tag="P5138_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59717.1"
                     /translation="MRAEFLKIRILPTPRWTLFALLLCFLAGVGFSVTSGVGHDEAVL
                     DLAVMLPSVICSIVFGSWMAGVEFGQNTLRRSLAADPRRVRLVLAKAAATVITVAAVT
                     VGLWALGMLVFPLLGSGHETTIDSGRAVGMGLAALLQNVVYALIALSLTLLTRSMGGG
                     MAMSFAFAFVISAFLSVIPKVGDYMVSSALE"
     gene            complement(564..1520)
                     /locus_tag="P5138_03755"
     CDS             complement(564..1520)
                     /locus_tag="P5138_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV59718.1"
                     /translation="MAESLIRLEGVSKRYGSVKALDGLSLEVPEGTVCGLLGPNGSGK
                     TTAIRVLLGLTAMNAGRAELLGAEPGSADFRLATGQTGSLIEGPALYQRASARQNMEI
                     QAATLGLDHAAGQIEELLRMVDLDGRADTRSGGFSLGMKQRLGLALALLGNPRLVILD
                     EPTNGLDPSGIVEIRELIKRLPERGITVLVSSHLLAEVQLMCDRATIIKDGRLVAGGT
                     MEEILSSTGVGEGYRVYVRISEAEAAAAALGRAGLEVEDRGEGSLVARGKIDDDSEIT
                     RTLAEAGIWLRGLMRERPDLERVFLTLTDTNASENGGQTDEG"
     gene            complement(1533..2738)
                     /gene="tgt"
                     /locus_tag="P5138_03760"
     CDS             complement(1533..2738)
                     /gene="tgt"
                     /locus_tag="P5138_03760"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRV59719.1"
                     /translation="MRHDPARLSFEIHARDGNARRGTLQTPHGPVETPAFIPLATKGS
                     VRGMSSREIEGMGYEMVLGNTYHLLVAPGPERIAEAGGLHRFMGWEKAIITDSGGFQV
                     FSLAHGGVAEEIKGSGRKMYGLAKTLGIEEEGVRFRSYKDGSELMLSPEESMRVQAAL
                     GSDIALVFDECTPFHADREYTARSTERTHRWLKRCLDWHEANGPSGQAVFGIIQGGTH
                     ADLRKESCEAVSASGVDGISIGGTLGRDKEEMAEVLGFTVPNLPVEAPKHLLGIGEVD
                     DLMRGVALGLDVFDCAVPTRLARHGMALAPLPDNRFRIDVRKPREIGNRDPLVPGCPC
                     TACAAYDRDYLNYISKSEELTAVRLLVDHNLTYMERLMRGAREAISDGEYSTYSEKIL
                     AGSTPWDAR"
     gene            complement(2762..>3315)
                     /locus_tag="P5138_03765"
     CDS             complement(2762..>3315)
                     /locus_tag="P5138_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRV59720.1"
                     /translation="VAETEKYPHVTYFFNGGREQEWEGEDRRLVDSPRDVPTYDFKPE
                     MSANQAADAFIAGWKEDEPRFGIINFANPDMVGHTGVIPAAVQAIEAVDACLGRVVDT
                     VLASGGACLITADHGNADNMLEPDGSPNTAHSLNPVPVIVTAEGFELAEGGILADVAP
                     TILEMLGIAQPAEMTGHSLIEPA"
BASE COUNT          588 a         1116 c         1054 g          557 t
ORIGIN      
        1 attcgagggc tgaactgacc atgtagtcac cgaccttcgg aataaccgaa aggaaggcgc
       61 tgatgacgaa ggcgaaagcg aatgacatcg ccatgccccc tcccatcgac ctggtcagca
      121 gggtgagact aagtgcgatc aacgcataga ccacgttctg aagcaacgca gcgagaccca
      181 tgccgaccgc ccgtccggaa tcgatcgttg tctcatgacc ggatccgagc agcgggaaga
      241 ccagcatgcc cagggcccag agcccgactg tgaccgcagc caccgtgatc accgttgccg
      301 ctgcctttgc cagaacaaga cggacccggc gcgggtcagc ggcaagtgac cggcgcaggg
      361 tgttctggcc gaattcgacc ccggccatcc aggaaccgaa cacgatcgaa cagatgacgc
      421 tcggcagcat cacggccagg tcgagcacgg cctcgtcgtg accgaccccc gacgtgacgc
      481 tgaagccgac cccggccagg aaacacaaca gcaatgcgaa aagggtccac cggggcgtcg
      541 ggagaatccg gatcttgagg aactcagccc tcatcggtct ggcctccgtt ttcactcgcg
      601 ttcgtgtcgg tcaaggtcag gaagacccgt tcgagatcgg ggcgctcgcg catcagaccg
      661 cgcagccaga tcccggcttc ggccagggtt cgcgtgattt ccgaatcgtc gtcgatcttg
      721 ccgcgggcaa ccaggctgcc ctcgccccgg tcctcgacct caagcccggc ccgcccaagg
      781 gcggccgccg ccgcctcagc ctccgagatt cggacatata cccggtaacc ctcgccaacg
      841 ccggtcgaag agaggatctc ctccatggtg ccaccggcga ccagccgccc gtccttgatg
      901 atcgtcgccc ggtcacacat caactgaacc tcggccagca gatgggaaga caccagaacc
      961 gttattccgc gctcggggag acgtttgatc aactccctga tttcgacaat tccggaaggg
     1021 tcgagaccgt tggtcggctc gtcgaggatc accaggcggg ggtttcccag cagggccagg
     1081 gcaagcccca ggcgctgctt catgccgagc gagaagccac cggaccgggt gtcggcgcgc
     1141 ccgtccagat caaccatcct gagcaactcc tcgatctggc ccgccgcgtg gtcaaggccg
     1201 agggtcgcag cctgaatctc catgttctgc cgggcggatg cccgctggta aagggcaggg
     1261 ccttcgatca gtgacccggt ctgacctgtg gcgagccgaa aatcggccga gccgggttcg
     1321 gccccgagca gttcggcccg gccggcgttc atcgcggtga ggccgagcag gaccctgatc
     1381 gcggtcgtct tgccggagcc gttggggccg agcagaccac agacggtccc ctcaggcacc
     1441 tcgagcgaaa gcccgtcgag tgcctttacg ctgccatagc gcttggaaac tccctccagc
     1501 cggatcaggg attcagccat ctgccgggca gcctaccggg catcccaggg ggtcgatccg
     1561 gccaggatct tctcgctgta ggtggagtac tccccatccg atattgcttc gcgggcgccg
     1621 cgcatcagcc gctccatata ggtcaggttg tggtcgacca ggagcctcac cgcagtcagc
     1681 tcctctgact tcgagatgta gttcagataa tcccggtcgt aggcggcgca ggcggtgcag
     1741 ggacagcccg ggaccagcgg atcgcgatta ccgatctccc ggggcttgcg gacgtcgatc
     1801 cggaaacggt tgtccgggag cggagccaga gccattccgt gtctggccaa ccgggtcggg
     1861 acggcgcagt cgaagacatc gagaccaagc gcgaccccgc gcatcaggtc gtcgacctct
     1921 ccgatcccga gcaggtgctt cggcgcctcg accggcaggt tcggcaccgt gaacccgagt
     1981 acctcggcca tctcttcctt gtctcgtccg agcgtgccgc cgatcgaaat cccgtccacg
     2041 ccggatgcgg aaacggcctc gcatgactcc ttgcgcagat cggcatgggt gcctccctga
     2101 atgatcccga acaccgcctg accggacggg ccgttcgctt catgccagtc gagacatcgc
     2161 ttcagccagc ggtgggtgcg ctcggtcgag cgggcggtgt attcgcggtc ggcgtgaaaa
     2221 ggggtgcatt cgtcgaagac cagggcgatg tcggaaccga gagcagcctg caccctcatc
     2281 gactcctccg gcgagagcat cagctcggac ccgtccttgt aggaacggaa ccggacccct
     2341 tcctcctcga tgcccagggt cttggcgagg ccgtacatct tgcgtcccga gcccttgatc
     2401 tcctcggcca cccctccgtg ggccagcgaa aagacctgga agccaccaga gtccgtgatg
     2461 atcgctttct cccagcccat gaagcggtgg agcccgccgg cttcggcgat ccgttcgggg
     2521 cccggcgcga ccagcaggtg gtaggtgttg ccgagcacca tctcgtatcc catcccctcg
     2581 atctcccggg aggacatgcc cctgaccgag cccttggtcg caaggggtat gaaggccggg
     2641 gtctcgaccg ggccatgcgg cgtctgcagc gtcccgcggc gggcattgcc gtcgcgggcg
     2701 tggatctcga agctcagccg agccgggtcg tggcgcatcc ggccacccta tccgtcgcgg
     2761 atcaggccgg ctcgatcagg ctgtgtccgg tcatctcggc gggctgggcg atgccgagca
     2821 tctcgaggat ggtcggggcc acgtcggcca ggatcccgcc ctcggcgagt tcgaatcctt
     2881 cggcggtgac gatcacgggg accggattca gcgagtgggc cgtgttcggc gacccgtccg
     2941 gttccagcat gttgtcggcg ttgccgtgat cggcggtgat caggcaggcg ccgccggagg
     3001 ccagcaccgt atcgacgacc ctgccgagac aggcgtcgac cgcctcgatc gcctgaaccg
     3061 cagccgggat cacgccggtg tgcccgacca tgtcggggtt ggcgaagttg atgatgccga
     3121 acctcggctc gtcctccttc cagccggcga tgaaggcgtc agcagcctgg ttggcgctca
     3181 tctccggctt gaaatcgtag gtcggcacgt cgcggggcga atcgaccagc cggcgatcct
     3241 ccccctccca ctcctgctcg cggccgccgt tgaagaagta ggtcacatgc ggatacttct
     3301 cggtttcggc gacat
//
LOCUS       DAOTIP010000145         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34079_length_3314_cov_2.868671, whole genome shotgun sequence.
ACCESSION   DAOTIP010000145 DAOTIP010000000
VERSION     DAOTIP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34079_length_3314_cov_2.868671"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /gene="ispH"
                     /locus_tag="P5138_03770"
     CDS             complement(<1..739)
                     /gene="ispH"
                     /locus_tag="P5138_03770"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRV59721.1"
                     /translation="MPETPSTGIEKVILAAPRGYCAGVDRAVQTVERALDNHGAPIYV
                     RKEIVHNKHVVEQLAARGAIFVEEETEVPEGEIVVFSAHGVSPAVHERAAARELRTID
                     ATCPLVTKVHVEARKFAAEGYTIIMVGHEGHEEVEGTMGEAPDSIVLVQTVDEVDHLE
                     IEDPDHVALITQTTLSVDETAGIIARLRERFPGIVTAKGEDICYATTNRQIAVKQLAR
                     QCDLVLVIGSTNSSNSNRLVEVAREHGS"
     gene            790..2331
                     /locus_tag="P5138_03775"
     CDS             790..2331
                     /locus_tag="P5138_03775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRV59722.1"
                     /translation="MEPASHELDRLTAIDAQFLTNEHENAHMHIGAVMIFEGDPPTYD
                     EFKAHIGSRLDFVPRYRQKLVYPPYGTGRPLWADDPDFNLNYHLRHPALPRPGGEREL
                     KILASRLFSQALDRSKPLWEMFLVEDLEGDRFAIIIKSHHAMIDGISGVDIGTVIFDV
                     TADAEPIVPKEEWKPKPLPSNLSLLERGAKELATEPVKLGEKAINALRKPSSLAGRVG
                     ESAEGLAEVARALADSAPDVPLNVPIGPHRRITWTSADLADYRRVKDVFGTTVNDVVL
                     AVAAGSIRKWLVKRGVDTDGLELRAMVPVSVRSDDERGQLGNKLATMRAPLPVYEADP
                     VARLEIVSAAMDGLKQSKQALGAEVIARLNDFAPPTLLAQAARLTFSTRLFNLLVTNV
                     PGPQIPLYVLGRKLDRPYPVAFLAKNHGLAVGVMSYAGQVNIGLIGDFDAMPDIGQVR
                     RGLNESLAELLAAAEEVENSIAPDKPGKASARKGSARKGSPGKATASNGGGPGRAAKK
                     SSPKG"
     gene            complement(2328..2645)
                     /locus_tag="P5138_03780"
     CDS             complement(2328..2645)
                     /locus_tag="P5138_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRV59723.1"
                     /translation="MAEDHATMPTEEEVFEALEEVIDPELGLDFVSLGLVYDVALEAP
                     DVYVTFTLTTPACPIGPQVSEQMKEFVGGLEGVRDVHPKMIFDPPWSPEMMTDDAKFA
                     LGF"
     gene            2790..>3314
                     /locus_tag="P5138_03785"
     CDS             2790..>3314
                     /locus_tag="P5138_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRV59724.1"
                     /translation="MFVLLLALLIIWPIAELFVMVQVADWIGFFWMLFLLFASTVAGI
                     MILKHRGRAHWRRFRGAIDERRPPAKEAFDGVMITAGALLLIIPGFITSAIGLFLLFP
                     PTRWLVRLVALTLFASKFKVAATTATWSTRGYNYYRGSRQGSYDIDGEAVDVTDRPDG
                     EPLPQLRSGSERGED"
BASE COUNT          550 a         1086 c         1073 g          605 t
ORIGIN      
        1 cggaaccatg ttcccgcgcc acctcgacca gccggttgga attggacgag ttggtcgagc
       61 cgatcaccag cacgaggtcg cattggcggg ccagctgctt gaccgcgatc tgacggttgg
      121 tggtggcgta acagatgtcc tcgcccttgg cggtgacgat tccggggaac ctttcgcgca
      181 ggcgggcgat gatcccggcg gtctcatcga ccgaaagggt ggtctgggtg atcagggcga
      241 cgtggtccgg atcctcgatt tcaaggtggt cgacctcgtc aacggtctgg accaggacga
      301 tcgagtccgg tgcctcgccc atcgtgcctt caacctcttc gtggccctcg tggccgacca
      361 tgatgatcgt gtagccctcg gccgcgaact tgcgggcctc gacgtggacc ttggtgacca
      421 gcgggcaggt cgcgtcgatc gtgcgcagct cccgcgccgc agcccgttcg tgaaccgccg
      481 gcgaaacgcc gtgggcggag aagacgacta tctcgccttc gggcacctcg gtttcctctt
      541 cgacgaagat cgctccccgc gccgctagct gctcgaccac gtgcttgttg tggacgattt
      601 ccttgcggac gtagatcggg gcaccgtggt tgtcgagcgc gcgctcgacc gtctgcacgg
      661 cccggtcgac gccggcgcag tagccacggg gcgcggccag aatgactttc tctatcccgg
      721 tggagggtgt ttcgggcatt ggacaagggt acccgagtag agtcttgtgc tagtccacga
      781 tggggagcca tggaacccgc cagccacgaa cttgaccgac tcactgcgat tgatgcgcag
      841 tttctgacca acgagcacga aaacgctcac atgcacatcg gtgcggtgat gatcttcgaa
      901 ggggatccgc cgacctatga cgagttcaag gcccacatcg gctcgcggct cgatttcgtg
      961 cccaggtacc gccagaagct ggtctatccg ccttacggca ccggccgtcc gctctgggcc
     1021 gatgatcctg atttcaacct gaactaccac ctgcgccacc ccgccctgcc gaggccgggt
     1081 ggcgaacgcg agctgaagat tctcgcctcg cggctcttct cgcaggccct ggaccgttcc
     1141 aagccgctct gggaaatgtt cctggtcgag gatctggagg gcgaccggtt cgcgatcatc
     1201 atcaagagcc accacgcgat gatcgacggc atctccgggg ttgacatcgg cacggtgatc
     1261 tttgacgtca ctgccgacgc cgagccgatc gtccccaagg aggaatggaa gccgaagccg
     1321 ctgccctcga atttgagcct gctggaacgg ggcgcgaagg aactcgcgac cgaaccggtg
     1381 aagctgggcg agaaggcgat caacgccttg cgcaagcctt cgtcgctggc cgggcgggtc
     1441 ggggaaagtg ccgagggact ggccgaggtg gcccgggctc tggccgattc cgcgcccgac
     1501 gtgccgttga acgtgccgat cggtccccat cgccggatca cctggaccag cgccgacctg
     1561 gccgactacc ggcgggtcaa ggacgttttc ggcaccaccg tcaacgacgt ggtgctggcg
     1621 gtcgccgccg gttcgattcg caagtggctg gtcaagcgcg gggttgacac cgatggcctt
     1681 gagctgcggg ccatggttcc ggtctcggtc cgctccgacg acgagcgcgg ccagctcggc
     1741 aacaagctcg ccacgatgcg ggccccgctg ccggtttacg aggccgatcc ggtcgcccgg
     1801 ctggagatcg tcagcgccgc gatggacggc ctgaagcagt ccaagcaggc gctgggggcg
     1861 gaagtgattg cccgactcaa tgacttcgcc ccgcccaccc tgctcgcgca ggcggcccgg
     1921 ctgaccttct ccacccggct tttcaatctg ctcgtgacca acgttccagg tccgcagatt
     1981 ccgctctatg tcctgggacg caaactcgac cggccctatc cggtcgcctt cctcgccaag
     2041 aaccacggcc ttgcggtcgg cgtgatgagt tacgccgggc aggtcaacat cggcctgatc
     2101 ggtgacttcg acgcgatgcc cgacatcggc caggtccgcc gcggcctgaa cgaatcgctg
     2161 gccgagctgc tcgccgccgc cgaagaggtc gagaactcga tcgcccccga caaacccggg
     2221 aaggcttcgg ccagaaaggg ttcagcccgg aagggcagcc ccgggaaggc cactgccagc
     2281 aacggcggcg gcccgggccg ggcggcgaag aagagcagcc cgaagggcta gaagccgagc
     2341 gcgaacttgg cgtcgtcggt catcatttcc ggcgaccagg gcgggtcgaa gatcatcttc
     2401 ggatgaacgt cgcgtacgcc ctcgagcccg ccgacgaatt ccttcatctg ctcggagacc
     2461 tggggtccga tcgggcaggc gggcgtggtc agggtgaatg tcacgtagac gtccggcgct
     2521 tccagcgcaa cgtcatagac gagccccagt gaaacgaagt cgagcccgag ttcggggtcg
     2581 atcacttcct cgagcgcttc gaaaacctct tcttcggtcg gcatggttgc gtggtcttca
     2641 gccatgaatg catcctagct gggtcgggat tccggccgcc agcccgtgct gcttcggccg
     2701 gtgggtcggg tttccggccg gggtgacgga aacccgaccc accgcaggct gtaacccgga
     2761 ttgcccgggt gcgtaacctg tatctgccca tgttcgtact gctcctggca ttgctgatca
     2821 tctggccgat cgcggaactt ttcgtgatgg tgcaggtggc cgactggatc ggcttcttct
     2881 ggatgctgtt cctgctcttc gcctcgaccg tggcgggcat catgatcctc aaacaccgcg
     2941 gcagagcgca ctggcgccgg ttccgcgggg cgatcgatga gcgacgaccg ccagcgaagg
     3001 aagccttcga cggggtgatg atcaccgccg gcgcgctgct tttgatcatc cccggcttca
     3061 tcacctcggc gatcggtctt ttcctgctct tcccgccgac caggtggctc gttcgcctgg
     3121 tcgcgctgac cctcttcgcc tccaagttca aggtcgcggc caccaccgcg acctggagca
     3181 ctcgtggcta caactactac cggggctccc gccagggttc ctacgacatc gacggcgaag
     3241 cggttgacgt gaccgaccgg cccgacggtg agcctctccc ccagctacgc tccgggtcag
     3301 agcgcggtga agac
//
LOCUS       DAOTIP010000146         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34165_length_3307_cov_3.018143, whole genome shotgun sequence.
ACCESSION   DAOTIP010000146 DAOTIP010000000
VERSION     DAOTIP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34165_length_3307_cov_3.018143"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="P5138_03790"
     CDS             <1..214
                     /locus_tag="P5138_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRV59725.1"
                     /translation="SRRLGSPLADQLRRQSATLRQDQRRAIEEQAARAAPKIQLVIAL
                     ILVPSVLMLIVAALAANVDSLIGVGY"
     gene            complement(370..1122)
                     /locus_tag="P5138_03795"
     CDS             complement(370..1122)
                     /locus_tag="P5138_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59726.1"
                     /translation="MSDSTQPDGKSPDSAGEGTVAVHHANQLRSAKFKSMMRKKSTWA
                     WLVGLSVVGGGILAAFAGPGIGLIGFLIIFAVILLVVFGIADRQAEDAFYDFYCESHG
                     LTRSMTPDIGELTPLLRKGDKTSVDEVFTGELAENIHGDLVLYTYTEVHRDSDGDETE
                     TDYPFTLIHVEMPEIIPHMTDLRVERASFKFLDGIEDKFLGDFERVTLESEAFSKRFE
                     VFVRKDQDAIWTRRLFSPSFIVWLTENPPDSF"
     gene            complement(1119..2009)
                     /locus_tag="P5138_03800"
     CDS             complement(1119..2009)
                     /locus_tag="P5138_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59727.1"
                     /translation="MGEITDANQLRGAKFKELLGKPKTWIILLVCSLIAAAGGMIIGP
                     AVAGGAFVLALLIGLVITLVIADRRAAKAFYEAYAEARGLTYHSGGGLGGSTPLLRKG
                     DKSRVDEQFNGMLADGIEGTLALYTYTVTSTDSDGNDTSTDYPFTVIMVNLPGTVEHM
                     TDLRVQKKFGLKMFEKLEDAFRRDFGRVTLESEALRDRYEIFVRKEQDPIWVRRLFSP
                     SFIVWLTDVPPKKFAFELERGWLCAYVPKHRDSTEGLDEMVKLGTTVAGRLKEEVDQS
                     SPAELHSDSPEIRIETKEEN"
     gene            2223..2660
                     /gene="mraZ"
                     /locus_tag="P5138_03805"
     CDS             2223..2660
                     /gene="mraZ"
                     /locus_tag="P5138_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRV59728.1"
                     /translation="MAFRGQHEHSLDSKDRLTVPRKFRAALDDGYVLAKGPDACLWLY
                     ATKDFDAMEERYVAPHSPFGADARNLRRMLNASADEGELDAAGRIRIPDDLKKLTGID
                     GACAVVGAGDYIEIWNAEAWAKLNEDLLGQFADVAENLSGGSE"
     gene            2696..>3307
                     /gene="rsmH"
                     /locus_tag="P5138_03810"
     CDS             2696..>3307
                     /gene="rsmH"
                     /locus_tag="P5138_03810"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRV59729.1"
                     /translation="MSDSRPGHGIPDRPDQGPRGHLPVLATELVELIGPQPGETVIDC
                     TFGAGGHARMVAERIGPEGTLVAIDRDPVAQARFREFEAEAPCRTRFIATEFSLALKS
                     LQNEGFRASALYMDLGMSSLQVDAAERGFSYSHDAPLDMRMDPRQSLTAEEILNTWSE
                     QQLSHMLRELGEERYAGGISRQIVARRPLETTSDLVEAIKAGMP"
BASE COUNT          568 a         1052 c         1081 g          606 t
ORIGIN      
        1 gtcacgccgg ctcggctcgc cgctggccga ccagctgaga cggcagtcgg cgaccctgcg
       61 ccaggaccag cggcgcgcga tcgaggagca ggccgccagg gcggccccga agatccagct
      121 ggtgatcgcg ctgatcctgg tgccgtcggt gctgatgctg atcgttgcgg cgctggccgc
      181 gaacgtcgac agcctgatcg gcgtcggcta ctgaccggct tccggtccgg tcctgggcga
      241 ggtctgcgcg acctcctcga gcaaccgggc agccaccttg gtgccgacgt cggtgacctt
      301 ctcgaggctc gcggtgtcat ccatgtgctt ggagacgaag gcgacgaggt gcccgttctc
      361 gagttcgaat taaaaggagt ccggcgggtt ctcggtcagc cagacgatga agctggggga
      421 gaacaggcgg cgggtccaga tcgcgtcctg gtctttccgc acgaagactt cgaagcgctt
      481 cgagaatgcc tcgctttcga gcgtcaccct ttcgaagtct ccgaggaact tgtcctcgat
      541 cccgtcgagg aacttgaagc tggccctttc gaccctgagg tcggtcatgt gcgggatgat
      601 ctcgggcatc tccacgtgaa taagggtgaa cgggtaatcg gtctcggtct cgtcaccatc
      661 ggaatcgcgg tggacctccg tataggtgta gagcaccagg tcgccgtgga tgttctccgc
      721 gagctcgccg gtgaacacct cgtccacgct cgtcttgtcg cccttccgca gcaggggagt
      781 gagctcaccg atgtccggag tcatgctccg ggtcagtccg tgtgattcac agtagaagtc
      841 gtagaaggcg tcctcggcct gccggtcggc gatgccgaag acgaccagga gaatcaccgc
      901 gaagatgatc aggaagccga tcagcccgat ccccgggccg gcgaaggcgg cgaggatgcc
      961 gccgccgacg accgagaggc cgaccagcca ggcccaggtc gacttcttcc gcatcatcga
     1021 cttgaacttg gccgagcgca gctgattggc gtggtgtacc gctacggtgc cctcaccggc
     1081 cgagtcgggg gattttccgt ccggttgcgt ggagtcgctc aattttcttc cttcgtttcg
     1141 atccggattt caggtgagtc ggaatgaagc tctgccggtg agctctggtc gacctcttcc
     1201 ttgagccgcc cggccaccgt ggttccgagt ttcaccatct cgtcgaggcc ttcggtgctg
     1261 tcacgatgct tcggtacata ggcgcaaagc cagcctctct ccagctcgaa ggcgaacttc
     1321 ttcggtggga cgtcggtcag ccagacgatg aaactcgggg agaagaggcg gcggacccag
     1381 atcggatcct gctccttgcg cacgaatatc tcgtagcggt ccctcagcgc ttcactttcc
     1441 agggtgaccc ggccgaagtc ccggcggaag gcgtcttcga gcttctcgaa catcttgagg
     1501 ccgaacttct tctggacccg caggtcggtc atgtgctcga ccgttcccgg caggttgacc
     1561 atgatcacgg tgaacggata gtcggtggag gtgtcatttc cgtccgagtc ggtgctggtg
     1621 accgtgtagg tgtagagcgc gagggttccc tcgattccgt cggcgagcat tccgttgaac
     1681 tgttcgtcaa cccggctctt gtcacccttg cgcagcagcg gggtggagcc acccaggccg
     1741 ccccccgaat ggtaggtgag gccccgggcc tcggcgtagg cctcgtagaa ggccttcgcg
     1801 gcccgtcggt cggcgatcac cagggtgatc accagtccga tcagcagcgc gaggacgaat
     1861 gcgccgcccg cgacggcggg gccgatgatc atccctccgg cggcggcgat caggctgcag
     1921 acgaggagga tgatccacgt cttcggtttg cccagcagct ccttgaactt ggcgccacgc
     1981 agctgattcg catccgtgat ttctcccatg gcggcagatt attcgatgcc cggccggtgc
     2041 aaccggcatt gcaggttttg gaagaaagtg ggtggttgtg ttgcaaagtg gggcgaagtg
     2101 ggttatgttg ccaatcgagt tgaagccaag ggccggggtg atggagctcc tcccgctctc
     2161 accaccccgg cccgactctt tcccggtcaa ccagcggcga gtcaaagaaa ggacgaggag
     2221 cggtggcgtt caggggccag cacgaacaca gcctcgattc caaggatcgc ctcacggtgc
     2281 ctcgcaaatt ccgtgccgcg ctcgacgacg gttacgttct cgccaaggga ccggatgcct
     2341 gcctctggct ctatgcgacc aaagacttcg atgcgatgga ggagcgctat gtggcgccgc
     2401 acagcccgtt cggcgccgat gcccgcaacc tccgccgcat gctcaacgcc agcgccgacg
     2461 aaggcgaact ggacgctgcc gggcgaattc gcattcccga tgacctcaag aaactgaccg
     2521 ggatcgacgg cgcctgcgcc gtggtcgggg ccggcgacta catcgagatc tggaatgccg
     2581 aagcctgggc caagctcaac gaagacctgc tcgggcagtt cgccgacgtg gccgaaaacc
     2641 tctcaggggg ctcggagtga ccgccgccgc gcggctcgcc ccgacaggag ccaaggtgag
     2701 cgactctcgt cccggccacg gaatccccga tcgtcccgac cagggtccgc gcgggcatct
     2761 gcccgtcctc gcgaccgaac tggtcgagct gatcggtccc cagccggggg agacggtgat
     2821 cgactgcacc ttcggcgccg gggggcacgc ccggatggtc gccgaacgta tcggcccgga
     2881 agggaccctg gtcgcgatcg accgcgatcc ggtcgcccag gcccggttcc gcgagttcga
     2941 ggcggaagct ccgtgccgga cccgcttcat cgcgaccgag ttcagcctgg cgctcaaatc
     3001 cctgcaaaat gaaggttttc gggcctcggc gctgtacatg gatctcggga tgtcttcact
     3061 tcaggtggac gccgccgaac gcggcttctc ctattcacat gacgcgcccc tggacatgcg
     3121 gatggacccg cgccagagcc ttaccgcgga agagatcctc aacacctggt ccgagcagca
     3181 gctcagccac atgctccgcg agctgggcga ggagcgctac gccgggggca tcagccgcca
     3241 gatcgtcgcc cgtcgcccgc tcgagacgac ttcggatctg gtcgaggcga tcaaggccgg
     3301 gatgccg
//
LOCUS       DAOTIP010000147         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34167_length_3307_cov_2.635916, whole genome shotgun sequence.
ACCESSION   DAOTIP010000147 DAOTIP010000000
VERSION     DAOTIP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34167_length_3307_cov_2.635916"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..648)
                     /locus_tag="P5138_03815"
     CDS             complement(136..648)
                     /locus_tag="P5138_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV59730.1"
                     /translation="MAMPGEQVREAYRAHSGELFGFACRSLGDRDGAEEAVQECFLKA
                     WRSRDSFDPALGSERTWLFAILRNVLVDMARSDSRRPAGTPLEQSSDRIPSAEQPFER
                     AMLAWQVEEALRRIGERHRQIVIETCIRGRPYAEVAAALGVPEGTIKSRVYYGLRAMR
                     DAMEELGYES"
     gene            complement(667..1239)
                     /locus_tag="P5138_03820"
     CDS             complement(667..1239)
                     /locus_tag="P5138_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59731.1"
                     /translation="MAVAVAGTLVLAGCGGGSDSDSSYSSNSSDSDSSSSAKTAYGDK
                     YGNPSSNSSAGSSTSGASGGSMAAGAAVVSTAKVGDLGTILVDGEGMTLYYFKKDDSG
                     KSACYGACAQAWPPLTSDGDPMTKDGAMADKLGTTKRTDGSSQVTYAGWPLYLYAGDQ
                     KPGQASGNDTHSFGGEWYALTPSGEKPEDS"
     gene            1527..>3307
                     /locus_tag="P5138_03825"
     CDS             1527..>3307
                     /locus_tag="P5138_03825"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRV59732.1"
                     /translation="MSIFETTRVETRVGDADISFETGKLAKQASGSVVVRSGDTMVLS
                     TATMGGLRDVDFLPLTVDVEERMYAAGKIPGSFFRREGRAGEKATLTARMIDRPIRPL
                     FPKGWHYETQLVTIPMSVDQEHPYDVLAMNGASAALAISEIPVAKHVGAVRIGKLDGD
                     FVINPNEEDHELLDLDLIVAGTDEAILMVEAGANGVTEADILDALDIAHGEIKKITAT
                     IEELRQKIGKEKLVIEEPQIDEGLLSDISGSHSQALVDAIATEGKLERYEAIDEVKDA
                     VVAQYAPDTGDEEADSARASEVKTAFAAIEKDTIRKQIAVDKKRPDGRAQDEIRPIEC
                     EVDVSPRVHGSALFTRGETQILGNVTLGTTRMDMRVDGLGLETTKTFWHHYNFPPFSV
                     GEAGFMRGPKRRDIGHGALAERALSPSVPDQEDFPYVIRAVSETLESNGSSSMGSVCA
                     SSMAMQAAGIPVSAPVAGVAMGLIKEGDDYIVLTDIAGVEDHLGDMDFKVAGTADGIN
                     ALQMDIKITGVTFDILTDALEQARKGRLFILGKMAEAIDGPREQLSPHAPRIESIKID
                     QEKIGAVIGKGGETIRGLCEEFEAEIDV"
BASE COUNT          610 a         1100 c         1024 g          573 t
ORIGIN      
        1 cccgactgtc tcagcgactc ggcttccgcc cggcattcgc tgcaggactc cagatgtttg
       61 ctgaccgtgg ctgcgttttc cgggtcgaga tggcccatcg cccatgctcc caggctctcg
      121 cggatctccc gatgctcagg actcatagcc cagttcctcc atcgcgtctc gcatcgctct
      181 gaggccgtag tagacccggc tcttgatcgt cccctcggga actccgagcg cggcggctac
      241 ctcggcgtag ggccggccac ggatacaggt ctcgatcacg atctgccggt gccgttcccc
      301 gatccggcga agggcttcct cgacctgcca ggcgagcatc gcccgttcga agggctgctc
      361 ggccgacgga atacggtcgg aagattgctc cagtggagtt ccggccggcc tgcggctgtc
      421 agagcgggcc atgtcaacga gcacgttgcg gagaatcgca aacagccagg tgcgctcgga
      481 gcccagcgcc ggatcgaagc tgtcccggct tcgccaggcc ttgaggaagc actcctgcac
      541 cgcttcttcg gccccgtccc ggtcgccgag cgaacggcag gcgaaaccga agagctcacc
      601 ggagtgcgcc ctgtaggcct cgcgtacctg ttcgcccggc attgccatcc gtcggttgcc
      661 tcctagctag ctatcttccg gtttctcacc gctcggggtc aaggcgtacc actcgccgcc
      721 gaaagagtgg gtgtcatttc cgctggcctg gccgggcttc tggtctccgg cgtagaggta
      781 aagcggccag cccgcgtaag tgacttgcga tgagccgtcg gtccgtttgg tggttccgag
      841 cttgtcggcc atggcaccgt ccttggtcat cggatcacca tccgaggtca acggaggcca
      901 ggcctgggca caggctccgt aacaggcgga tttaccgctg tcgtccttct tgaagtagta
      961 gagcgtcatt ccttcgccat cgacgaggat cgtgcccaga tcgccgactt tcgcggtgga
     1021 gaccactgcc gcgccggctg ccatcgatcc gccggacgcg cccgaagtcg acgaaccggc
     1081 ggatgaattc gaggacggat tgccgtactt gtcgccgtaa gcggtcttgg ccgagctcga
     1141 cgagtccgaa tcggaggagt tcgaggagta gctcgaatcc gaatcgctgc cgccaccgca
     1201 tccggccagg acgagggttc cggctacggc gaccgccaaa gtcgccaggc cccgacgata
     1261 gaaaaccggt gaatctttca taaccgggat tacgtcctgc accggaaaag gttcacctac
     1321 ccgtgctgcg gaaaaacgag atggcagaag tgggcgatta tggcccgctt ctgccttctc
     1381 gatccgccag ggacgcgatt tccggaaatt gcggggtaag gcgcgacttc gggcctcgcc
     1441 ctgtaccctt gtggggataa gggaacgaca gttctagaag acagatggcc gccacccggg
     1501 cggccggagg aaaaacgaat aacccaatga gtattttcga aacgactcgc gttgaaacgc
     1561 gggtcggcga cgccgatatc tccttcgaga ccggcaagct cgccaagcag gccagcggtt
     1621 ccgtcgtagt acgcagcggc gacaccatgg tcctctcgac cgccacgatg ggtggtctgc
     1681 gcgatgtgga cttcctgccc ctgaccgtcg acgtcgaaga gcgcatgtac gccgcgggca
     1741 agatccccgg ctccttcttc cgtcgcgaag gtcgcgccgg cgagaaggca accctgaccg
     1801 cccgcatgat cgaccggccg atccgtccgc tcttccccaa gggctggcac tacgagaccc
     1861 agctggtcac catcccgatg tcggtcgacc aggagcatcc ctacgacgtc ctcgccatga
     1921 acggtgcttc ggccgccctg gcgatctccg agattccggt cgccaagcac gttggtgcgg
     1981 ttcgcatcgg caagctcgac ggtgacttcg tgatcaaccc gaacgaggaa gaccacgagt
     2041 tgctcgacct cgacctgatc gtcgccggta ccgacgaggc catcctgatg gtcgaagcgg
     2101 gcgccaacgg cgtcaccgag gccgacatcc tcgacgccct tgacatcgcc cacggcgaga
     2161 tcaagaagat caccgcgacc atcgaggaac tgcgccagaa gatcggcaag gagaagctgg
     2221 tcatcgaaga gccgcagatc gacgagggcc tgctctccga cattagtggt tcgcacagcc
     2281 aggctctggt tgacgccatc gccaccgaag gcaagctcga gcgctacgag gcgatcgatg
     2341 aggtcaagga cgccgtcgtg gcccagtacg caccggacac cggtgacgaa gaggccgatt
     2401 cggcccgcgc ttccgaggtc aagaccgctt tcgccgcgat cgagaaggac acgatccgca
     2461 agcagatcgc cgtcgacaag aagcgccccg acggccgcgc ccaggacgag atccgtccga
     2521 tcgagtgcga ggtggacgtc tcgccccgcg ttcacggttc cgccctgttc acccgtggcg
     2581 agacccagat cctcggcaac gtcaccctcg gcaccacccg aatggacatg cgggtagacg
     2641 gtctcgggct ggaaacgacc aagaccttct ggcaccacta caacttcccg ccgttctcgg
     2701 tgggcgaggc cggcttcatg cgcggcccga agcgccgcga catcggtcac ggtgccctcg
     2761 ccgagcgcgc cctgtcgcct tcggttccgg atcaggagga cttcccctac gtgatccgcg
     2821 ccgtctccga gacgctggaa tcgaacggtt cctcgtcgat gggctcggtc tgcgcctcat
     2881 ctatggccat gcaggccgcc ggcatccccg tttccgcgcc ggttgccggt gtggcgatgg
     2941 gcctgatcaa ggaaggcgac gactacatcg tcctcaccga catcgccggt gtggaagacc
     3001 atctcggtga catggacttc aaggtcgccg gtaccgcgga cggcatcaac gccctccaga
     3061 tggacatcaa gatcaccggt gtcaccttcg acatcctgac cgacgcgctc gagcaggccc
     3121 gcaagggtcg gctgttcatc ctcggcaaga tggccgaggc gatcgacggt ccgcgcgagc
     3181 agctctcacc gcatgccccg cgcatcgagt cgatcaagat cgaccaggag aagatcggcg
     3241 ccgtcatcgg caagggtggc gagaccatcc gcggtctctg cgaggagttc gaggccgaga
     3301 tcgacgt
//
LOCUS       DAOTIP010000148         3304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34205_length_3304_cov_2.480148, whole genome shotgun sequence.
ACCESSION   DAOTIP010000148 DAOTIP010000000
VERSION     DAOTIP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34205_length_3304_cov_2.480148"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="P5138_03830"
     CDS             complement(<1..779)
                     /locus_tag="P5138_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59733.1"
                     /translation="MGSDPTGASGAIRRQPWLGLFILALCLSAALLIALRANIGFFLD
                     DWGLVIYREGGPTDWLLPHNEHIIVLPAALYKLSLAVFGMTAFPLHMIAVALFLTSVV
                     LLFFWLRPLIGDPTAVLGCAVVLFLGAAAADLIWAFQIGYFGSVAGGLGALLLLRRGN
                     SGDSVWACLLLVLALLFSSLAIPFALGAAVWLLFPNGPRPEWNGFFRRSWVFLVPAGV
                     YVIWWLGWGHLAENSMSVHNAVRDPLYVLSAIGYAASVLVGA"
     gene            830..1885
                     /locus_tag="P5138_03835"
     CDS             830..1885
                     /locus_tag="P5138_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV59734.1"
                     /translation="MKTALVTGSGGLIGSQSVIKLVESGWHVIGIENDMRASFFGEEA
                     STRPVSERLASTLDEFEPVELDIRDAEGLDRVFAANRVELVVHTAAQPSHDWAARDPQ
                     TDFGVNALGTLNLLEAARAHAPDAPFIFCSTNKVYGDTPNFLPLIETETRLELPEDHE
                     YYDGIDTSMSIDHSTHSLFGVSKASADLLVQEYGRYFDMPTVCFRGGCLTGPQHAGAE
                     LHGFLAYLMKCVVTGRPYTVFGYGGKQVRDNIHCDDLVSAFLEFAAAPRTAAVYNIGG
                     GRGSNCSMLEAIAESEQIAGRELDWTMSEENRIGDHRWWISDLGAFESDYPNWERRFG
                     ITEILEQIHEYAVQAAA"
     gene            complement(1895..>3304)
                     /locus_tag="P5138_03840"
     CDS             complement(1895..>3304)
                     /locus_tag="P5138_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59735.1"
                     /translation="MFGIGSPRPMQIASTATFVATGLLLFVWLRLRVGDWGALIGAAI
                     VLFLGAAFEDLLWAFQIGYFGSLACGIGALIALDREDRKGDIAAAILLVASISFSSLG
                     IPFALGAVTEWLMNPRERGRRWFVPAAPIVFYALWWLGWGHEAESAVSLSNVPDLPKY
                     VFDAASAAMTSMLGLATGDGSEPDQPHLIWGRLALVVLIGLAAWRLIRLGKVPRGVAV
                     TATIALAFFALAALGHNDLRPPTSSRYQLPAVILILLFCGELLRGMKIPGYAFLAATV
                     VVAVTSFSGVGLMRDQARDRWKPSAISNQVTLGAIAIAGDAARPNFVLNLSSVSVPIA
                     RFQDEVASSGSPGYSDAELARMDAAYRGLADQTLIDTEGLILSGTGPALTGSGCQTTT
                     PKTGGAVAVFPSENPVLIINRENTDLEVSLARFGDPPGSPIGSIYPGSRAWLTLPPDD
                     STRPWQISLGGRGAVRVCD"
BASE COUNT          653 a         1060 c         1110 g          481 t
ORIGIN      
        1 gccccgacca ggacgctggc cgcgtagccg atggcggaaa ggacgtagag cggatcccgg
       61 accgcgttgt ggacgctcat cgagttttcg gcaaggtgcc cccagccgag ccaccagatc
      121 acatagacgc cggcgggaac caggaagacc cacgaccgtc ggaagaaacc gttccattcc
      181 ggtcgcggcc cgttcggaaa gagaagccag acggctgcac ccagggcaaa agggatcgcg
      241 agggaggaaa acagcagggc cagaaccagc agcaggcagg cccagaccga gtcaccgcta
      301 tttccccggc gcaagaggag gagggcaccg agtccaccgg caacggagcc gaagtagccg
      361 atctgaaagg cccagatgag atctgcggcg gccgcgccga ggaagagcac caccgcgcag
      421 ccgagcaccg cggtcgggtc accgatcagg gggcgcagcc agaagaagag cagcaccaca
      481 ctggtcagga aaagggcgac ggcgatcatg tgcaggggga acgccgtcat cccgaaaacc
      541 gccagcgaga gtttgtaaag cgcggccggc aggacgatga tgtgctcgtt gtgtggaagc
      601 agccagtcgg tcgggccgcc ttcccggtag atgaccaggc cccagtcgtc gaggaagaag
      661 ccgatgttcg cccggagggc gatcagaagg gccgccgaaa ggcacagggc gagaatgaaa
      721 aggcccagcc acggctgcct gcggatggcc cctgaggcgc cggttgggtc cgatcccatg
      781 ttgttaggtt acttcgcatg cttgcgactc ccccggccag gagggcctaa tgaaaaccgc
      841 gttggtgacc ggttccggcg gactcatcgg gtcccagtcg gtgatcaagc tggtcgagtc
      901 cggctggcat gtgatcggca tcgaaaacga catgagggcc agtttcttcg gcgaggaggc
      961 atcgacccgc ccggtcagcg agcggctcgc gtcgaccctg gacgagttcg agccggttga
     1021 actggacatc cgcgacgcgg aaggcctgga tcgggtcttc gcggcgaacc gggtggaact
     1081 ggtggttcac acagcggccc agccttccca tgactgggcg gcccgcgacc cccagaccga
     1141 cttcggggtg aatgccctgg gaacgctgaa cctgctcgag gcggcgcgcg cccacgcacc
     1201 tgacgcccct ttcatcttct gctccaccaa caaggtctat ggcgacaccc cgaacttcct
     1261 gccgctgatc gagaccgaga cccggctcga gttgccggag gatcacgagt actacgacgg
     1321 gatcgacacc tcgatgtcga tcgatcactc gacccattcc ctgttcgggg tttccaaagc
     1381 cagcgccgac ctgctggtcc aggagtacgg ccgctacttc gacatgccta ccgtctgctt
     1441 ccggggtggc tgcctgaccg ggccccagca cgccggggcc gagctgcatg gttttctcgc
     1501 ctacctgatg aagtgcgtgg tgaccggccg gccctacacc gtgttcggtt acggcggcaa
     1561 gcaggtccgc gacaacatcc actgcgacga cctggtttcg gcctttctcg aattcgctgc
     1621 cgctccccgc acggcggcgg tctacaacat cggcggcggt cgcggcagca actgctcgat
     1681 gctggaggcg atcgccgaat cggagcagat cgccggtcgt gaactcgact ggaccatgag
     1741 cgaggagaac cggatcgggg accatcgatg gtggatctcg gacctgggag ccttcgagag
     1801 cgactacccg aactgggagc gccgcttcgg gatcaccgag atcctcgagc agattcacga
     1861 gtacgcggtt caggccgccg cctgaaccgg attttcaatc gcagacccga acggccccgc
     1921 ggccccccag actgatctgc cagggcctgg tggaatcgtc cggcggcaag gtgagccagg
     1981 cccgtgaacc cgggtagatc gatccgatcg ggctgcccgg agggtcaccg aaacgggcca
     2041 ggctcacctc caggtccgtg ttttcccggt tgatgatcaa caccggattc tccgaaggga
     2101 acacggcaac cgcaccgccc gtctttgggg tcgttgtctg gcagccggaa ccggtaagtg
     2161 ccggacccgt gcccgagagg atcagcccct cggtgtcgat cagggtctgg tcggccagtc
     2221 cccggtaggc agcatccatc cgggcgagtt ccgcgtccga gtagccgggg gatccgctgc
     2281 tcgccacctc atcctgaaag cgggcgattg gcactgacac ggaactgagg ttcagcacga
     2341 agttcggccg ggcggcgtcg cccgcgatcg cgatcgcacc cagcgtcacc tggtttgaga
     2401 tggcgctggg cttccaccgg tcccgggcct gatcacgcat cagtccgacc ccggaaaagg
     2461 acgtcacggc gaccacgacg gtggccgcca ggaaggcgta gccggggatc ttcatccctc
     2521 gcaacagttc cccgcagaac agcaggatca gaatcactgc cggcaactgg taccggctcg
     2581 aggtcggggg acggagatcg ttgtgaccga gggcggccag ggcgaagaag gcaagcgcga
     2641 tggtcgcggt caccgcgact ccccggggca cttttccgag ccggatcaga cgccaggcgg
     2701 cgagcccgat cagaacgacc agggcaaggc gcccccagat cagatggggc tggtccggtt
     2761 cgctgccatc gccggtggcc aggccgagca tcgatgtcat cgccgccgat gccgcgtcaa
     2821 agacgtactt cggcaggtcg gggacgttgg agaggctgac cgcgctctcg gcctcgtgac
     2881 cccagccgag ccaccagagg gcgtagaaaa cgatcggcgc agcgggtacg aaccagcgtc
     2941 gtccgcgctc cctcggattc atcagccatt cggtcacggc acccagggcg aaggggatcc
     3001 cgagcgagga gaacgagatg gaggcgacga gcaggatcgc ggctgcgatg tctcccttcc
     3061 ggtcctcgcg gtcaagcgcg atcagagcgc cgattccgca ggccagcgag ccgaagtagc
     3121 cgatctggaa tgcccagagc agatcctcga aggccgcccc tagaaagagc acgatggccg
     3181 cgccgatcag tgctccccag tcaccgaccc ggagccggag ccagacgaag aggagcaggc
     3241 cggttgccac gaaagtggcg gtcgaggcga tctgcatcgg gcggggggag ccgatcccga
     3301 acat
//
LOCUS       DAOTIP010000149         3300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34245_length_3300_cov_2.763328, whole genome shotgun sequence.
ACCESSION   DAOTIP010000149 DAOTIP010000000
VERSION     DAOTIP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34245_length_3300_cov_2.763328"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="P5138_03845"
     CDS             <1..514
                     /locus_tag="P5138_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59736.1"
                     /translation="RSKQHRLSSRASQKVLGWTAGTALAFLVLCGFGVQRYYFDHRYA
                     SPDFTIPALSEAFVWANGESGVKIGTNATRQYPLWGEDLDNEVQFIGLNEPHAGFVEA
                     ENCRDFIAAANQGDYDYLVLTLDREGQKLAQPRELTWVSKDPAVKYVVDAEGGAVLSL
                     DGPLSPRSCR"
     gene            543..2336
                     /gene="lepA"
                     /locus_tag="P5138_03850"
     CDS             543..2336
                     /gene="lepA"
                     /locus_tag="P5138_03850"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRV59737.1"
                     /translation="MDTTRNFSIIAHIDHGKSTLADRILELTKAVDPLKMRAQVLDSM
                     DLERERGITIKAQAVRVEFTASDGVTYHLHLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQTVANTYLAIENGLELIPVLNKVDLPGAEPERVAGEVSDLIGVDPDDILR
                     ISAKTGEGVIDVLEAVVDRIPPPEGEPDAPARALIFDSEFDQYRGVIAYVRMMDGRFR
                     KHGKVLAMQNGTEAELDDIGFFRPVMTPVDEIGTGEVGYVITGIKDVAKLRVGDTLTN
                     LENPAAEALAGYRDVKPMVFCGLFPIDTDRYEDLREALDKMGLNDAALSWEPETSEAL
                     GFGFRCGFLGLLHMEIVRERLEREYGLELLATTPTVRYEVHLTSGEMTEISSPTEMPD
                     PGSIERIEEPYIRATVITPSDYVGPVMELCQSRRGTHVDMDYLSPTRVQIRYDLPLAE
                     IVLDFFDVLKSSTKGYASLDYEPIGLRASDLVKVDVLLAGEKVDALSVITHKDFAYNQ
                     GKDLVSRLRKTIPRQQFDIPVQAAIGANILARETVKAVRKDVTAKLYGGDVTRKRKLL
                     EKQKAGKKRMKNVGRVEVPQEAFLAVLELDE"
     gene            2367..2813
                     /locus_tag="P5138_03855"
     CDS             2367..2813
                     /locus_tag="P5138_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRV59738.1"
                     /translation="MALKTDAVGKSWPAYEYEVGREKIREYAQVIGFDNPVYFDAEAA
                     RAAGYRDIVAPPMFVVVYAAGAMGPVLFDPDVEMNFAAMVHGGQEFEWGEPVCSGDLI
                     TTSTTCKDIYPKDGKGFYVFETESKNQNGDLVCKATWTNIVRGIEE"
     gene            2810..3205
                     /locus_tag="P5138_03860"
     CDS             2810..3205
                     /locus_tag="P5138_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRV59739.1"
                     /translation="MSAKEKGEKIAGLKVTPDKFLPHRYAGASGDFNPIHIDKEFAQM
                     VGLPTTILHGLYVMGLVAKAATEEAGGDPRALKRLSVQFRGMSMPETEIEVSGEVIEA
                     DGADVTFDLIAAQGENQVIRNASAVISYP"
BASE COUNT          648 a         1063 c         1045 g          544 t
ORIGIN      
        1 ccggtcgaag cagcaccggc tttccagtcg tgcttcgcag aaggtcctcg gctggaccgc
       61 tggcacagcc ctggcctttc tcgttctctg cggtttcggc gttcagcgct actacttcga
      121 ccaccgctac gcatcccccg atttcacgat cccggccctt tccgaggctt tcgtctgggc
      181 caacggcgag tcgggcgtga agatcggcac caacgcgacc cgccagtacc cgctctgggg
      241 ggaggatctc gacaacgagg tccagttcat cgggctgaat gaacctcacg ccggtttcgt
      301 cgaagcggag aactgccggg atttcatcgc ggcagccaac cagggtgact atgactacct
      361 ggtcctgacc ctcgaccgag aaggccagaa actggcccag ccacgcgaac tgacctgggt
      421 gtcgaaggac ccggcggtca aatacgtggt cgacgcagag ggaggagcgg ttctttcgct
      481 agacggacct ctctcgccgc gatcctgccg ataggcgcct ccggcatact gctctgtccg
      541 tgatggacac gacccgcaac ttctcgatca tcgcccacat cgaccatggc aagtcgacgc
      601 tggccgaccg catcctcgag ctgaccaagg ccgtggaccc gctcaaaatg cgggcccagg
      661 tactggactc gatggatctc gagcgcgaac gcggaatcac gatcaaggcc caggccgtga
      721 gggtcgaatt caccgcctcg gacggggtga cctaccacct ccacctgatc gacacgccgg
      781 gccacgtgga cttctcatac gaggtttcgc gctctctggc cgcctgtgaa ggcgccctgc
      841 tcgtggtgga cgccgcgcag ggcgtcgaag cgcagaccgt ggcgaacacc tatctggcga
      901 tcgaaaacgg actggaactg atccctgtcc tgaacaaggt cgacctgccc ggcgccgagc
      961 cggagcgagt ggccggtgag gtctccgacc tgatcggggt ggaccccgac gacatcctcc
     1021 ggatctcggc caagaccggg gagggagtga tcgacgtgct cgaagcggtc gtagaccgta
     1081 tcccgccgcc cgagggcgag ccggacgcac cggcccgggc gcttatcttc gactcggaat
     1141 tcgaccagta caggggcgtc atcgcctatg tccggatgat ggacgggcgc ttccgcaagc
     1201 acggaaaggt gcttgcgatg cagaacggca ccgaggccga actcgacgac atcggcttct
     1261 tcaggccggt catgaccccg gtcgacgaga tcggaaccgg cgaggtcgga tacgtgatca
     1321 ccggcatcaa ggacgtggcc aagctgcggg tcggcgacac tctgaccaac ctcgagaacc
     1381 ccgcggcaga agccctggcc ggctaccggg acgtgaagcc gatggtcttc tgcgggctct
     1441 tcccgatcga caccgaccgc tacgaggacc tgcgggaagc gctcgacaag atggggctga
     1501 acgacgctgc tctttcgtgg gagccggaaa cctcggaggc cctcgggttc ggcttccgct
     1561 gcggctttct cgggctgctc cacatggaaa tcgtgcgcga acgacttgag cgggagtacg
     1621 ggctcgagct gctcgccacc actccgaccg tgcgttacga ggtacacctg accagcggcg
     1681 agatgaccga gatctccagt ccgaccgaaa tgccggaccc cggctcgatc gaaaggatcg
     1741 aggagcccta catcagggcc accgtgatca ccccgagcga ctacgtcggc ccggtgatgg
     1801 agctctgcca gtcccgccgc gggacccacg tggacatgga ctacctgtca ccgacccggg
     1861 tccagatccg ttacgacctg ccgctggccg agatcgtgct cgactttttc gacgtgctca
     1921 agtcctcgac caagggttat gcctcgctcg actacgagcc gatcggcctc agggcatcgg
     1981 acctggtcaa ggtggacgtg ctgctggccg gcgagaaagt ggacgcgctc tcggtcatca
     2041 cccacaagga cttcgcctac aaccagggca aggacctggt ttcccgcctc cgcaagacga
     2101 tcccccggca gcagttcgac atcccggtcc aggcagcgat cggggccaac atcctcgccc
     2161 gcgagaccgt caaggcggtc cgcaaggacg tcaccgcaaa gctctacgga ggtgacgtga
     2221 cccggaagcg caagctgctc gagaagcaga aggccggcaa gaagcggatg aagaacgtgg
     2281 gccgggtcga ggtcccccag gaagccttcc tcgcggtgct ggagctcgac gagtagcccg
     2341 atcttccgcg ggtatagatt tccggcatgg cgctgaagac cgatgccgtt ggaaaaagct
     2401 ggccggccta cgaatatgaa gtgggtcggg aaaaaatccg cgaatacgcg caggtaatcg
     2461 ggttcgacaa cccggtgtac ttcgacgccg aggcagcccg ggcggccggt taccgcgaca
     2521 tcgtcgcgcc gcccatgttc gtggtcgtct acgcggccgg ggcgatgggc ccggtgcttt
     2581 tcgatccgga cgtggagatg aacttcgccg cgatggtcca cggtggccag gaattcgaat
     2641 ggggcgagcc ggtctgttcc ggtgacctga tcaccacctc gaccacctgc aaggacatct
     2701 acccgaagga cggcaagggc ttctacgtct tcgagaccga gtcgaaaaac cagaacggtg
     2761 acctcgtctg caaggccacc tggaccaaca tcgtcagggg aattgaagaa tgagcgcaaa
     2821 ggaaaagggc gagaagatcg ccgggctgaa ggtcaccccg gacaagttcc tgccgcatcg
     2881 ctacgccggt gcctccgggg atttcaaccc gatccacatc gacaaggaat tcgcccagat
     2941 ggtcgggctg cccaccacga tcctgcacgg cctctatgtc atgggcctgg tcgccaaggc
     3001 cgcgaccgaa gaggccggcg gagacccgcg ggccctgaag cggctctccg tgcagttccg
     3061 cgggatgagc atgcccgaga ccgagatcga ggtttccggc gaggtgatcg aggctgacgg
     3121 tgccgacgtg accttcgacc tgatcgccgc ccagggcgaa aaccaggtca ttcgcaacgc
     3181 ttccgccgtc atcagttacc cgtagtccag gggcgccgga gccggcgcat tcatacaatt
     3241 gaccgatgct ctccgagcgg caagaactga ttcttgggct ggttgtggac gcctaccggg
//
LOCUS       DAOTIP010000150         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34547_length_3278_cov_3.950977, whole genome shotgun sequence.
ACCESSION   DAOTIP010000150 DAOTIP010000000
VERSION     DAOTIP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34547_length_3278_cov_3.950977"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="P5138_03865"
     CDS             complement(<1..1298)
                     /locus_tag="P5138_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59740.1"
                     /translation="MKTFLTSLGALLVGLLICSSSASAQNMLFWTDLENNEVMSMPLD
                     GSQPPGVLDISPASVDADQQSADPGSLDYDPVTGRLYWPNAGNPGDVSWAAADGSGGG
                     TVNTTANPIDPPTALSLDGGNDRLYIVGGDGESVATMGLGGTQGTWALNDGDFRNGIL
                     VDARSSTLWIAGPEWVSYGNLDGTGTLDTYSPNAFTNGGFSVDRDADRLYGTWYPGAG
                     EPDVLSWMATDASTDGSLTPGAVDVFGASSTAIDHDTGEIYWANAYPFGEADTRGIFR
                     MPLSGGAGEQVSPGQIGGRSGGLVILKAPETTSDLELTGESELGSELTCGDVTWAGDR
                     PEAHYFRSPVEKKLVWVINGEQSDATDGNSVQADEPGTYQCVRAGQNAAGIGAAESNE
                     IVIPNPTPVCPTIRLSVRISKFTPPRPFGHHNAPGVRVTFR"
     gene            complement(1572..>3278)
                     /locus_tag="P5138_03870"
     CDS             complement(1572..>3278)
                     /locus_tag="P5138_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRV59741.1"
                     /translation="KKGWKVFNDLRQHTNAESPTSIDGKFPYEQLPKKSRKGSVVLDP
                     GSYTAYNAVQGMSLSAERSSNALEPRHQASNTLMITKKKSATGRPLMVGGPQIGYFAP
                     GLTYEIDMNAPGLKWRGATSAPFPGYLLIGRGQDFATTLTSASGDVIDQFVETLCGGS
                     DTKYLYKGKCRNMGTFDAGTLNGNPVSFKTTVHGPVVGYATVKGKKVAISSKRSSYGK
                     DVLDLLFNRRLSNGSVKGPNSFFEAASKTPQTFNSFYIDNKNIALYTSGKLPIRDKRV
                     DPGLPTKGTGQYEWKGFLGKKKHPHGKNPKSGMMVNWNNSVAHGFGSADDEWGRAGSV
                     QRVDLLTRMLNRNKNKKTGKLNMAQVVSAMNAGATQDVRAIFTVPLLAKLLKGSKAPN
                     SQARQMLNQMVDWNKKNGSRLDRNLDGMIDAPGAASLDAAWPKIADAFMKPVLGSQLD
                     ELGSLFSRFDLPPSGQYSGWYQYFDKDIRALLGKKVKSPFRVKYCGHGKKAACQKAVW
                     AAIAAAGTELQADQGSANPADWHADATREQIKFGPVSLITMRYTNRPSGIQQVISFNG
                     HR"
BASE COUNT          507 a         1032 c         1081 g          658 t
ORIGIN      
        1 cggaaggtga cccggactcc cggggcgttg tgatggccga agggcctcgg cggcgtgaac
       61 ttcgagatcc ggaccgaaag cctgatcgtc ggacacaccg gcgtcgggtt gggaatgacg
      121 atctcgttgc tttcggcagc accgatgccg gccgcatttt gtcctgcccg gacgcactgg
      181 taggtccccg gctcgtcggc ctgcacgctg ttgccgtcgg tcgcgtcgct ctgttcgccg
      241 ttgatcaccc agaccagctt tttctcgacc ggactgcgga agtagtgggc ctcgggccgg
      301 tcgccggccc aggtcacatc accacaggtc agttccgatc cgagctcgga ctcccctgtc
      361 agttcaagat cagacgtcgt ttccggagcc ttcaggatca ccagcccgcc gcttcggccc
      421 ccgatctggc cgggactgac ctgttccccc gcccctccgg aaagcggcat cctgaagatt
      481 ccacgcgtat cggcctcgcc gaatgggtat gcattcgccc agtagatctc accggtgtcg
      541 tggtcgatcg cagttgacga tgccccgaac acatccacgg cgccgggggt gagcgaaccg
      601 tcggtgctgg catcggtggc catccaggag agaacatccg gttcgcctgc tcccgggtac
      661 caggtgccgt agagccgatc ggcatcccga tcgaccgaaa aacccccgtt cgtgaacgcg
      721 ttcggtgaat aggtgtcgag cgtgccggtc ccgtcgaggt ttccgtagct gacccactcg
      781 ggaccggcga tccacagcgt cgaggaacgg gcgtcgacga gaattccgtt gcgaaagtca
      841 ccgtcgttca gggcccaggt cccctgtgtt ccgcccaggc ccatcgtggc cacgctctct
      901 ccgtcgccac cgactatgta gagccggtcg tttcccccgt ccagcgaaag ggccgtgggc
      961 gggtcgatcg ggtttgccgt cgtgttgacg gtgccgccgc cagaaccatc cgctgccgcc
     1021 caggacacat cgcccggatt tccggcgttc ggccaataga ggcggcccgt taccgggtcg
     1081 tagtcgagcg aaccgggatc agccgactgc tggtcggcgt cgaccgaggc cggactgatg
     1141 tcgagcacgc ctggcggctg gctgccgtcg aggggcatac tcatgacctc gttgttttcg
     1201 aggtcggtcc agaacagcat gttctgggcc gaggcgctcg acgagcaaat cagaagcccg
     1261 accagaaggg ctccaaggga agtcaagaag gttttcatcg ttcctctccc gggtgcctgt
     1321 tctcctgttc cgatactagc ccgaaccctg accctgcgaa tgcggcaggc gggaaatgtg
     1381 cgaaacgcca aggttccggt tttcagttcg aaatccgggg taatcggaga tcacggcctt
     1441 catttcaccg gggcgatccc cctgcgtccc ggtggatagg cctggcggtg agtgcctgca
     1501 ccgccatgga aagaggaaag cccgccccaa ggattggggc gggctttctc gattccggct
     1561 ttcggcggct cctagcggtg accgttgaag gagatcacct gctggatgcc ggacggccgg
     1621 ttggtgtacc tcatggtgat caggctcacc gggccgaact tgatctgctc gcgggtggcg
     1681 tcggcgtgcc agtcggccgg gttcgcagaa ccctggtcgg cctgcagctc cgtgcccgcg
     1741 gcagcgatcg cggcccagac cgccttctgg caggcggcct tcttgccgtg accgcagtac
     1801 ttgacgcgga aggggctctt caccttcttg ccgagcagag cccggatgtc cttgtcgaag
     1861 tactggtacc agcccgagta ctgaccgctc ggcggcaggt cgaaccgcga gaacagcgag
     1921 ccgagctcgt cgagctgcga tccgaggacc ggcttcatga acgcatcggc gatcttcggc
     1981 caggcggcat caagcgaagc cgcacccggg gcatcgatca tgccgtccag gttgcggtcc
     2041 agccggctgc cgttcttctt gttccagtcg accatctggt tcagcatctg cctggcctgg
     2101 ctgttcgggg ccttggagcc cttcagcagc ttggccagca gcggcacggt gaagatggca
     2161 cgcacgtcct gggtggcgcc ggcgttcatc gccgagacga cctgggccat gttcagcttg
     2221 ccggtcttct tgttcttgtt ccggttgagc atccgggtca ggaggtcgac ccgctggact
     2281 gaaccggcgc ggccccattc gtcatcggcg gagccgaagc cgtgggcgac gctgttgttc
     2341 cagttgacca tcattcccga cttcgggttc ttgccgtgcg ggtgcttctt cttgccgagg
     2401 aagcccttcc actcgtactg gccggtgccc ttggtcggca ggcccggatc gacccgcttg
     2461 tcgcggatcg gcagcttgcc gctggtgtag agggcgatgt tcttgttgtc gatgtagaac
     2521 gagttgaagg tctgaggcgt cttcgaggcg gcctcgaaga acgagttcgg acccttcacg
     2581 gagccgttcg aaaggcgacg gttgaagagc aggtcgagca cgtccttgcc gtagctggag
     2641 cgcttcgagc tgatcgccac cttcttgccc ttcacggtcg cgtaaccgac aaccggtccg
     2701 tggacggtcg tcttgaacga gaccgggttg ccgttcagcg tgccggcgtc gaaggtgccc
     2761 atgttgcggc acttgccctt gtacaggtac ttggtgtcac tgccaccaca gagggtttcg
     2821 acgaactggt cgatcacgtc gccgctggcc gaggtcagcg tcgtcgcgaa gtcctggccg
     2881 cggccgatca gcaggtaacc ggggaacgga gccgaggtgg caccgcgcca cttgaggccc
     2941 ggggcgttca tgtcgatctc gtatgtgaga ccgggggcga agtagccgat ctgcggtccg
     3001 ccgaccatca gcggacggcc ggtggccgac ttcttcttcg tgatcatcag ggtgttggag
     3061 gcctggtggc gcggctcgag cgcgttgctg ctgcgctcgg cgctcagcga catgccctgg
     3121 accgcgttgt aggcggtgta gctgcccggg tcgaggacga cgctgccctt gcggctcttc
     3181 ttcggcagct gttcgtaggg gaacttgccg tcgatcgagg tcgggctctc ggcgttggtg
     3241 tgctggcgca ggtcgttgaa gaccttccag cccttctt
//
LOCUS       DAOTIP010000151         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34556_length_3278_cov_3.076016, whole genome shotgun sequence.
ACCESSION   DAOTIP010000151 DAOTIP010000000
VERSION     DAOTIP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34556_length_3278_cov_3.076016"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="P5138_03875"
     CDS             <1..832
                     /locus_tag="P5138_03875"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRV59742.1"
                     /translation="EVTSGEGSISVDGKVIKVLAERDPADLPWGDLGVDVVLESTGFF
                     TKRDDAAKHLTAGAKKVIISAPATDPDVTVALGVNFDDYDPANHNIISNASCTTNCLA
                     PFAKAINDAVGIEQGLMTTIHAYTSDQRLQDMPHKDLRRARAAAQNLIPTTTGAAKAV
                     GLVLPDLAGKLNGFAVRAPVITGSVVDLTFTPSRATSVEEVNAAVKAAAEGPLKGILG
                     YTEDPIVSTDIVGDPHSSIFDAGCTQAIGDGTLIKAVSWYDNEWGYSNRCVELLGKVL
                     "
     gene            857..2044
                     /locus_tag="P5138_03880"
     CDS             857..2044
                     /locus_tag="P5138_03880"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRV59743.1"
                     /translation="MAFDKASVTGADVDGKRVLVRVDFNVPLEGERVTDDTRIRAALP
                     TIDFLTGAEAKVILCSHLGRPKGQVVPELSLAPAAKRLSELTGKPVEMAADVGGEDSQ
                     AKAEALAPGGILLLENTRYEPGETDNDPELAARLASLADLYVNDAFGAAHRAHASTAG
                     VATDLPAYAGLLLEKEVENLTSVASDPKRPLVVVLGGAKVSDKVGVIDRFLEIADQIL
                     IGGAMCFPFYKAQGHSIGGSLCHDDDIPLAREALAKSAAGNCELVLPTDLALGREFSA
                     DTDTEMLDGVDVPDNWMGLDIGPKSAADYAGRVASAGTVLWNGPMGAFELDPFADGTR
                     TVAEAVAAAPGFTVVGGGDSVAALVKFGLTGDVDWVSTGGGASLELLEGKELPGVAAL
                     DDK"
     gene            2065..2823
                     /gene="tpiA"
                     /locus_tag="P5138_03885"
     CDS             2065..2823
                     /gene="tpiA"
                     /locus_tag="P5138_03885"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRV59744.1"
                     /translation="MSRRPYVAANWKMNKTASEADEFLDSFLPTQSADGAEVVLCPPW
                     LALQRVAERCAGTPVKVCAQNMFYEDSGAFTGEVSAPMLLDVGVDGTILGHSERRQLF
                     GETDEALARKIPKAIEAGLEPILCVGETQNERESGDTESVLSHQVLTDLAEIHTDDLE
                     KVVIAYEPVWAIGTGLTASPDQAQDACEFIRNLLRSRDPKKAEGVRILYGGSVRPGNA
                     GELLALPDIDGALVGGAALDPDDFGAIVAAAPVE"
     gene            2842..>3278
                     /locus_tag="P5138_03890"
     CDS             2842..>3278
                     /locus_tag="P5138_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRV59745.1"
                     /translation="MSNLPVPSAALIILDGWGIAPPGPGNAISQADTPVFDRFWAQYP
                     TTQLSAQGPDVGLPEGQMGNSEVGHLNLGAGAIVKQDLARIDTAVADGSFFDNDSLVA
                     ACQRAVNGNGRLHLIGLVSDGGVHSGWEHLEACVELTTQEGVP"
BASE COUNT          575 a         1117 c         1069 g          517 t
ORIGIN      
        1 cgaagtgacg tccggcgagg gatcaatctc ggtcgacggc aaggtgatca aggtcctggc
       61 cgaacgcgat ccggccgacc tgccctgggg tgacctcggc gtcgacgtgg tgctcgaatc
      121 aaccggtttc ttcaccaagc gtgacgacgc agccaagcac ctgaccgccg gcgcgaagaa
      181 ggtcatcatc tcggccccgg ctaccgaccc ggacgtgacc gtggccctcg gcgtcaactt
      241 cgacgactac gacccggcca atcacaacat catctccaac gcctcctgca ccaccaattg
      301 cctggccccg ttcgccaagg cgatcaacga cgcggttggc attgagcagg gcctgatgac
      361 cacgatccac gcctacacct cggaccagcg cctccaggac atgccgcaca aggacctccg
      421 gcgggcccgc gccgccgcgc agaacctgat cccgaccacg accggggccg ccaaggccgt
      481 cggtctggtc ctgcccgatc tggccggcaa gctgaacggc tttgcggtcc gcgcgcccgt
      541 gatcaccggt tcggtcgtcg acctgacctt cactccttcc cgcgctacct cggtagaaga
      601 ggtcaacgcc gcggtcaagg ccgcggccga gggcccgctg aagggcatcc tcggctacac
      661 cgaggacccg atcgtttcga ccgacatcgt cggtgatccg cactcgtcaa tcttcgacgc
      721 cggctgcacc caggcgatcg gcgacggcac actgatcaag gcggtgtcct ggtatgacaa
      781 cgagtggggc tactcgaacc gctgcgtcga gctgctcggc aaggtcctct aggaagcttc
      841 cagcgaggag ctgaaaatgg ctttcgacaa ggcgtcggta acgggcgccg atgtcgacgg
      901 caagcgggtt ctggtccggg tcgacttcaa cgtccctctc gagggggaac gggtcaccga
      961 tgacacccgg atcagggccg cgctgccgac catcgacttt ctgaccgggg cggaggcgaa
     1021 ggtcatcctc tgctcccatc taggccgccc caaggggcag gtcgtgccgg aactttcgct
     1081 cgcaccggcc gcaaagcgcc tgtccgaact gaccggcaaa cccgtcgaaa tggccgccga
     1141 cgtcggcggc gaagactcgc aggcgaaggc agaagcactg gcgccagggg gaatcctcct
     1201 gctcgagaac acccgctacg agccgggcga gacggacaac gacccggaac tcgcggcgag
     1261 gctggcttcg ctggccgacc tgtacgtgaa cgatgcgttc ggagccgccc accgggctca
     1321 cgccagcacg gcgggggtcg ccaccgacct tcccgcgtat gccggtctgt tgctggagaa
     1381 ggaggtcgag aacctgacct cggtcgcatc cgaccccaaa cgaccactgg tggtggtgct
     1441 gggtggagcc aaggtttcag acaaggtcgg ggtgatcgac cggttcctcg aaatcgccga
     1501 ccagatcctg atcggcgggg cgatgtgctt ccccttctac aaggcccagg gccattcgat
     1561 cgggggctcg ctctgccacg acgatgacat tccgctcgcc cgtgaggcgc tcgccaagtc
     1621 cgccgccggc aactgcgagc tggtcctgcc gaccgatctg gcgctgggca gggagttttc
     1681 cgccgacacg gacacggaga tgctggacgg agtcgacgtt ccggacaact ggatggggct
     1741 cgacataggg ccgaaatcgg ccgccgacta cgccggacgg gtcgcctcgg ccggcacggt
     1801 gctctggaac ggaccgatgg gcgctttcga gctcgatcct ttcgctgacg gaaccaggac
     1861 cgtggccgaa gcggtcgccg cggcgccggg cttcacggtg gtcgggggcg gagactcggt
     1921 cgctgctctg gtgaagttcg gcctgaccgg tgacgtggac tgggtctcga ccgggggcgg
     1981 ggcttcgctg gaactgctcg agggcaagga acttccgggc gtcgccgccc tggacgacaa
     2041 gtaacgaatg gaaagaggca gtaattgagc cgtcgaccct atgtagccgc caactggaag
     2101 atgaacaaga ccgcctccga ggccgacgag ttccttgact ccttcctgcc aacccagtca
     2161 gccgacggtg ccgaagtcgt gctctgcccg ccgtggctcg cccttcagcg ggtggccgag
     2221 cgctgcgccg gcaccccggt caaggtctgc gcccagaaca tgttctacga ggattccggc
     2281 gccttcacgg gcgaggtatc ggctccgatg ctcctcgacg tcggcgttga cggcacgatc
     2341 ctcggccact cggagcgtcg ccagctcttc ggcgagaccg atgaggcgct ggcccggaaa
     2401 atcccgaagg cgatcgaggc cggtctcgag ccgatcctct gcgtcggaga gacccagaac
     2461 gagcgcgagt cgggtgacac cgaatcggtg ctttcccatc aggtcctgac cgatctcgcc
     2521 gagatccaca ccgacgacct ggagaaggtc gtgatcgcct acgagccggt atgggcgatc
     2581 ggcaccggcc tcaccgcatc cccggaccag gctcaggacg cctgtgagtt catccgcaac
     2641 ctgctgcgtt cccgggaccc gaagaaggcc gagggcgtcc gcatcctcta cggcggctcg
     2701 gtcagaccgg gcaacgccgg ggagctgctg gccctgcccg acatcgacgg cgccctggtc
     2761 ggcggagccg ccctcgatcc cgacgatttc ggcgcgatcg ttgccgcggc gccggtcgag
     2821 taggagactt tccggtcagc catgtcaaac ctgcctgtac cttccgccgc actgatcatc
     2881 ctcgacggct ggggaatcgc cccgccaggt cccgggaacg cgatctccca ggccgacacc
     2941 ccggtattcg accggttctg ggcgcagtac ccgacaaccc agctctccgc ccagggtccg
     3001 gacgtgggcc tgccggaagg ccagatgggc aattccgagg tcgggcacct caacctcggt
     3061 gcgggtgcca tcgtcaagca ggacctggcc cggatcgaca ccgcggtggc cgacggctcc
     3121 ttcttcgaca acgattccct ggttgccgcc tgtcagcgtg cggtcaacgg caacgggcgg
     3181 ctccacctga tcggcctggt ctcagacggg ggagtccatt cgggctggga gcatctcgag
     3241 gcctgcgtcg aactcacgac ccaggagggc gtaccgga
//
LOCUS       DAOTIP010000152         3274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34608_length_3274_cov_3.541783, whole genome shotgun sequence.
ACCESSION   DAOTIP010000152 DAOTIP010000000
VERSION     DAOTIP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34608_length_3274_cov_3.541783"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1790)
                     /locus_tag="P5138_03895"
     CDS             complement(66..1790)
                     /locus_tag="P5138_03895"
                     /inference="COORDINATES: protein motif:HMM:NF004526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HRV59746.1"
                     /translation="MDLLELRDKVVLITGGARGIGFETARLARERGASVALIDLEREV
                     VEESAVALGGDSIGIAADVTDLGQMEDAVAQTVERLGRVDVVIANAGITPPKTTVRAL
                     DPAIWEKVVEVNAVGVYNTARAAIDQVIANQGHMVLIASVYAHVNGVINTSYAASKAG
                     VEALGRTLRAELEPHGASAGVAYFGFVETDLISEIFDNELADSFRAEIAPSFLTGKMP
                     VSQVAEALVDGIGRREPRIVEPPAWRPPLYLRGQVGPMADRQLERNPVVGDYIREFER
                     RDIGESAAPVFPPVRSGDPYNLGGKVVLVTGAARGIGFEIARQAHGRGASVAVLDLDP
                     AEAEQAAARIGVRAVGFGADATSSDDLEKAFAGTREHFGRIDVVVANAGIAAKVAGTV
                     SVQDPDEWRRIYEVDMFGVFRTVRAGMEDVVKNGGQFVLISSSYAFMNGFLNSAYATA
                     KAGVEALGRGLRAELTPLGASATVAYYGWIDTELVAGAFEDPLVDRLRKDVTPGWMTR
                     RVPPAKAGKVVIDALEKRRPRAIAPAEWKGLFYGRGLLMPAFDKLLDGDAAVAEIVPA
                     AEERLRGD"
     gene            1862..>3274
                     /locus_tag="P5138_03900"
     CDS             1862..>3274
                     /locus_tag="P5138_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV59747.1"
                     /translation="MSSFRPVDPKQSFPEMEERINAVWKQNRVFERQLEQREGAEVWS
                     FYEGPPTANGRPGSHHVLSRVFKDIYPRYRAMRGYRVPRKAGWDCHGLPVELEVEKQL
                     GISSKQEIEEFGIEEFNRLCRESVFTYVEEWNRLTDRIGFWIDLDDPYVTMDSEYIES
                     VWWSLKELYESDRLYEGHRVSPYCPRCGTALSSHEVAQGYKDVTDPSVYVRFPLTDRD
                     ASLLIWTTTPWTLPGNYAVAVDPGVTYAEVEIDGETLILADKLIEKVLGEGVTAERTF
                     KGEELLGLGYEAPFPGMAENSGVFRVISGDFVTTDDGTGMVHIAPAFGEDDYRAAIAN
                     GLYDPSSRGPEGTLFNPVGLDGKFDSRVTGFEGEFVKDPEITAGLIDHLRKAGRLFRA
                     SDYEHSYPHCWRCGTPLLYYAKASWYIKTSEARDTMLAGNEKIGWHPEHIKEGRFGRW
                     LENNVDWALSRDRYWGTPLPI"
BASE COUNT          583 a         1120 c         1024 g          547 t
ORIGIN      
        1 gccgagatcg ttcccgcggc agaggaacgc ctccgcggcg accaaccacc tgtaagaacc
       61 tgcggtcagt cgccgcggag gcgttcctct gccgcgggaa cgatctcggc gacggccgca
      121 tctccgtcca ggagcttgtc gaaggccggc atgagcagac cgcggccgta gaaaaggccc
      181 ttccattcgg ccggagcgat cgcgcgcgga cggcgcttct cgagagcatc gatcacaacc
      241 ttgccggcct tggccggagg caccctgcgg gtcatccagc ccggcgtcac gtctttgcga
      301 agccggtcga ccaatggatc ttcgaaggcg ccggcgacca gctccgtgtc gatccagccg
      361 tagtaggcga cagtggcgct cgccccgagc ggtgtcagct cggcacggag accacgcccg
      421 agcgcctcga ccccggcctt ggcggtcgca taggccgagt tcaggaatcc gttcatgaag
      481 gcgtatgacg aagaaatcag cacgaactgt ccgccgttct tcaccacgtc ctccatgccg
      541 gcccggacgg tacggaagac cccgaacatg tcgacctcgt agatccgccg ccactcgtcc
      601 ggatcctgga cgctgactgt gccggcgact ttcgctgcga ttccggcgtt ggcaacgacc
      661 acgtcgatcc ggccgaagtg ctcgcgggtg cccgcgaaag ccttttccag gtcatcgctg
      721 ctggtcgcgt cggcaccgaa gccgaccgcc ctcaccccga ttcgggccgc tgcctgctcg
      781 gcttcggcgg ggtccagatc gagtaccgcc acggaagcac cacgaccgtg ggcctgacgg
      841 gcgatctcga atccgatccc cctggccgca ccggtgacca ggaccacctt gccgccgagg
      901 ttgtagggat ccccggaacg gaccggcggg aagaccggcg cggccgactc cccgatgtcc
      961 cgtctttcaa actcgcggat gtagtcgccg accaccgggt tgcgttcgag ttgccggtcg
     1021 gccatcgggc cgacctgacc gcgcaggtag agcggcggcc gccaggcggg cggctcgacg
     1081 atccgcggct cgcgccggcc gatgccgtcg accagcgctt ccgcgacctg gctcacgggc
     1141 atcttgccgg tcaggaacga gggcgcgatc tcggcccgga agctgtcggc cagctcgttg
     1201 tcgaagatct cgctgatcag gtcggtctcg acgaaaccaa aataggcgac cccggcggaa
     1261 gcaccgtggg gttcgagctc ggcccggagc gtccggccga gagcttcgac tccggccttt
     1321 gacgcggcgt aggaagtgtt gatcactccg ttgacatggg cgtagaccga ggcgatgaga
     1381 accatgtggc cctggttcgc gatcacctgg tcgatcgcag ccctcgccgt gttgtagacg
     1441 ccgaccgcgt tgacctcgac gaccttttcc cagatcgcgg ggtcgagggc cctgaccgtt
     1501 gtcttcggcg gggtgatgcc ggcgttggcg atcaccacgt caactctccc gagccgttcc
     1561 acggtctggg cgaccgcgtc ttccatctgg ccgagatcgg tcacgtcggc cgcgattccg
     1621 atcgagtcac cacccagcgc gaccgcagat tcctcaacga cttcgcgctc gagatcaatc
     1681 aacgcgaccg aggcgccccg ctcgcgggca agcctcgcgg tttcgaaccc gattcccctc
     1741 gctccaccgg tgatcagaac taccttgtcc ctcaactcaa gcaagtccat tgactcaccc
     1801 tagatggtcg cagcccgatc aaccctccgg gggcagcggc ctttcataaa ctcccctgct
     1861 catgtcgtca tttcgcccag tcgatccgaa acagtccttc cccgaaatgg aggagaggat
     1921 caacgcggtc tggaagcaga accgggtctt cgaacggcag ctcgaacagc gggagggagc
     1981 cgaagtctgg agcttctatg aagggccgcc gaccgccaac ggcaggccgg gctcgcacca
     2041 tgtgctcagc cgcgtcttca aggacatcta cccgcgctac cgggcgatgc gcggctaccg
     2101 ggtaccccgc aaggcgggct gggactgtca cggcctgccg gtcgagcttg aagtggaaaa
     2161 gcagctcggc atctcctcga agcaggagat cgaggagttc ggaatcgagg agttcaaccg
     2221 cctctgccgc gagtcggtct tcacctacgt cgaggaatgg aaccggctga ccgaccggat
     2281 cggcttctgg atcgatctcg acgacccgta cgtgacgatg gactccgaat acatcgagtc
     2341 ggtctggtgg tcgctcaagg agctctacga atccgaccgt ctctatgagg gccaccgggt
     2401 ctcgccctac tgcccgcgct gcggcaccgc actttcctcc catgaggtag cccagggata
     2461 caaggacgtg accgacccct ccgtctacgt ccgctttccc ctgaccgacc gcgacgcatc
     2521 actcttgatc tggaccacga ctccctggac gctccccggc aactacgccg tggcggttga
     2581 tcccggggtg acctacgccg aggttgaaat tgacggtgag accctgatcc tggccgacaa
     2641 gctgatcgag aaggtgctcg gcgagggagt cacggccgag cggaccttca agggcgagga
     2701 gctgctcggg ctgggctacg aagcgccctt ccccggcatg gccgagaact ccggcgtttt
     2761 cagggtgatc agcggcgatt tcgtcaccac cgatgacggc accgggatgg tccacatcgc
     2821 gccggccttc ggcgaggacg actaccgggc cgcaattgcc aacggtctct acgacccgtc
     2881 cagccgcggg ccggaaggca ccctcttcaa cccggtcgga ctcgacggga agttcgactc
     2941 cagggtgacc ggcttcgagg gtgagttcgt caaggacccg gagatcaccg ccgggctgat
     3001 cgaccatctc cggaaggcag gccgcctgtt ccgggccagc gactacgaac actcctaccc
     3061 gcattgctgg cgttgtggca ccccgctgct ctactacgcg aaggccagct ggtacatcaa
     3121 gacctccgag gcacgggaca cgatgctggc cggcaacgag aagatcggct ggcatcccga
     3181 gcacatcaag gagggtcgct tcggacgctg gctcgagaac aacgtcgact gggcactctc
     3241 ccgcgaccgt tactggggca ctcccctgcc gatc
//
LOCUS       DAOTIP010000153         3274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_34610_length_3274_cov_3.263436, whole genome shotgun sequence.
ACCESSION   DAOTIP010000153 DAOTIP010000000
VERSION     DAOTIP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34610_length_3274_cov_3.263436"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="P5138_03905"
     CDS             complement(<1..1430)
                     /locus_tag="P5138_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59748.1"
                     /translation="MLALFGAGLFAVSPAQAAGNPDLQLSASASNPLYGQTGTVSATA
                     SLANGQPKGYNLSFRIVLPAGISYAGGGQYVPTVIANQPATEQTTLLFNNISDLVPNS
                     SQSVSFDVNHDQALFDVGSTYGIQYEAFINSDPRIMPSFDASGQPVPASSTGTAAKNT
                     TSTVNAIKISKSEPSREGEILRGVHNHQTIYTLKVQNNYVNPTNGTKLNDWLPADLEF
                     LGCEGDPDNTTNAPTNPGSPDEYPGSGPIVVQPVPDCHKPSLVETVMADPDGAGPMPL
                     AVYTHVQWDTGDLAPGQEITYRYRAAVPLTENTLTWSGATPSTASGAQGANLDNNAVA
                     PPKEIQDEQKIVNYAVASGEYQSNTVPFNVSDETTLERTAEDLVVYKSNLSSPQLGEG
                     DITQWELRFRTGEYRYSDDIVVTDTLPSGLCPLGPVNYTTGNDGSDSECAPTGDNPSV
                     PYKSVNENADGTFTIVWDKTVMAKLG"
     gene            complement(1747..2652)
                     /locus_tag="P5138_03910"
     CDS             complement(1747..2652)
                     /locus_tag="P5138_03910"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRV59749.1"
                     /translation="MEASPQYTLTERGPLRAALKTARPQEWVKNVLVFAGVIFSGQLD
                     DTWAIGQALITFACFCAISSAGYFINDLNDVELDRQHPKKRFRPIAAGQLPISAAWVI
                     SAVLAVGAIAVAFFVVNWQVGCMVAGYGVIQVAYSFGLKQVVIIDVMTLATLFILRVM
                     AGAEAVEAHASEWLILCTGMLACFLGFTKRRQEAVSELHEGTSSRPVLEHYSLPFLDQ
                     MVAMVTTGTVVSYSIYTVNSPLAGSEMMLTIPPVVYGIFRYLYLIYDRSDDRSTAAIV
                     AEDRGVIIAGAVFVAVALLVLYAFD"
     gene            2855..>3274
                     /locus_tag="P5138_03915"
     CDS             2855..>3274
                     /locus_tag="P5138_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HRV59750.1"
                     /translation="MELTYQEGAITPEARDQLVEDLTTNLLKAEGAPNTEFFRSVTWV
                     LINERPAQLTYAGDRPGAGVVKLDVVTPEGALNDERRGQMIQAGTATIRDAFGIADQD
                     ALKVWVLCRDVVDGGWGAGGNVIHYKELVEIAKAQREA"
BASE COUNT          588 a          950 c         1126 g          610 t
ORIGIN      
        1 tggccgagct tggccatgac ggtcttgtcc cagacgatcg tgaaggtgcc gtcggcgttc
       61 tcgttgacgc tcttgtacgg aacgctcggg ttgtcgccgg tcggggcgca ctcggagtcg
      121 ctgccgtcgt ttccggtcgt gtagttgacc ggcccgagcg ggcaaagacc ggagggaagg
      181 gtgtccgtga cgacgatgtc atcgctgtag cggtactcgc cggtccggaa ccggagctcc
      241 cactgggtga tgtcgccctc gccgagctgg ggagaggaaa gattcgactt gtagacgacg
      301 aggtcctcgg ctgtgcgctc gagggtggtc tcatctgaca cgttgaaggg aacggtgttg
      361 ctctggtact caccggaggc gaccgcgtag ttgacgatct tctgctcgtc ctggatttcc
      421 ttcggtgggg caaccgcatt gttgtccagg ttggcaccct gggcgccgga ggcagtcgac
      481 ggggtggcgc cactccaggt cagcgtgttc tcggtcagcg gtaccgcagc gcggtagcgg
      541 taggtgatct cctgtccggg agcgaggtcg ccggtgtccc actggacgtg cgtgtaaacg
      601 gcgagcggca tcggtccggc accgtcgggg tcggccatca cggtttccac cagtgatggc
      661 ttgtggcaat cgggcacggg ctggaccacg atcgggccgg aacccgggta ttcgtccggt
      721 gagcccggat tggtcggcgc gttggtggtg ttgtccgggt cgccctcgca gccgaggaac
      781 tcgagatcag cgggcagcca gtcgttcagc ttggtgccgt tggtcgggtt tacgtagttg
      841 ttctggacct tcagcgtgta gatcgtctgg tggttgtgga caccgcgcag gatctcgcct
      901 tcccggctgg gttccgactt ggagatcttg atcgcgttga cggtcgaagt ggtgttcttc
      961 gctgcggttc cggtcgaact cgcgggtacg ggctggccgg aagcgtcgaa cgacggcatg
     1021 atccggggat ccgaattgat gaaggcctcg tactggattc cgtaggtgga gccgacatcg
     1081 aagagagcct ggtcgtgatt cacgtcgaag ctgaccgact gcgacgaatt cggaaccagg
     1141 tccgagatgt tgttgaagag gagggtcgtc tgttcggtcg ccggctggtt ggcgatgacg
     1201 gtcgggacgt actggccgcc gccggcgtag gagatgccgg ccggaagcac gatccggaag
     1261 gacaggttgt agccctttgg ctgtccgttg gcgagcgaag cggtcgcgct caccgtgccg
     1321 gtctggccgt agagcgggtt cgaggcactg gccgagagct ggaggtcggg gttaccggcc
     1381 gcctgggccg gagaaacagc gaaaagaccc gcgccaaaga gggcgagcac agcaagaatt
     1441 cgaacagcgg cgcgtacaga cggtttcaat ttttctccta gactctgggt cacctgatcg
     1501 gtacccggac aatttccaat tggcctggtg cgaacggtgg gaaagcctcg aaggcccggt
     1561 catctgccgc aagaatcgag aatttgcaga tgacagggtt tttcacggtg tccccggctt
     1621 cccgccggtt ctcgatatgg acccgaaccc gtctcccgtg ttctttggga ctactgacaa
     1681 tacaaacacg gcagcccgtg tcaagtttca cctcaccgtg caagaaccgc aaaaagcggg
     1741 gttcgctcag tcgaacgcgt agagcaccag cagggccact gccacgaaca ctgcgccggc
     1801 gatgatcacg ccgcggtcct cggccacgat cgccgctgtg gaacggtcat ccgaacggtc
     1861 gtagatcagg tagaggtagc ggaagattcc gtatacgacc gggggaatcg tcagcatcat
     1921 ctcggagccg gccaggggcg agttgaccgt gtagatcgag tagctgacca cggttccggt
     1981 ggtcaccatg gccaccatct ggtccagaaa cggcagcgag tagtgctcga ggaccggacg
     2041 gctgctggtt ccctcgtgaa gttccgagac ggcttcctgg cgtcgcttgg tgaagccgag
     2101 gaagcaggcg agcatgccgg tgcagagaat cagccattcg gaagcgtggg cttcaactgc
     2161 ttcggcaccg gccatcaccc gcaggatgaa cagggtggcc agggtcatca cgtcaatgat
     2221 cacgacctgt ttgaggccga agctgtaggc gacctggatc actccgtagc cggcgaccat
     2281 gcagccgacc tgccagttga ccacgaagaa cgcgaccgcg atcgcgccga ccgccaggac
     2341 tgccgagatc acccaggccg cgctgatcgg cagctgcccc gccgcgatcg gccggaaccg
     2401 cttcttcggg tgctgccggt cgagctcgac gtcgttcaga tcgttgatga agtagccggc
     2461 actcgagatc gcgcagaagc aggcgaaggt gatcagggcc tggccgatcg cccaggtgtc
     2521 atcgagctgg ccggaaaaga tcaccccggc gaagaccagg acgttcttga cccactcctg
     2581 gggccgggca gtcttgagcg cggcccgcag gggcccgcgc tcggtcagtg tgtattgggg
     2641 ggaagcttcc atcggtcggg ggggttccgt accccgactt cgggatacgg ccggggaagg
     2701 ctatcggcaa tggccgcggc acatgttgtc gtggccgtcg gtgaacccgg tcaatcactt
     2761 gacccggcac ctccggatcg gttatgcata cctgcatagt cggtccgatc ccggaccgag
     2821 tcttcccgga ccaaggagaa atccgatgcc tttgatggaa ctgacctacc aggagggggc
     2881 gatcaccccc gaggcccgcg atcagctggt cgaagacctg accaccaacc tgctcaaagc
     2941 cgagggggct ccgaacaccg agttcttccg tagcgtcacc tgggttctga tcaacgagcg
     3001 gccggctcag ctcacctatg ccggtgaccg cccgggggcc ggtgtggtca agctcgatgt
     3061 cgtcaccccg gagggcgccc tcaacgacga gcgccggggg cagatgatcc aggccggaac
     3121 cgcgacgatt cgtgatgcgt tcggcatcgc cgaccaagac gcgctcaagg tctgggtgct
     3181 ctgccgcgac gtggtcgacg gcggttgggg agcgggcgga aacgtcattc actacaagga
     3241 actggtcgag atcgccaagg cccagcgcga ggcg
//
LOCUS       DAOTIP010000154         3223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_35357_length_3223_cov_3.213068, whole genome shotgun sequence.
ACCESSION   DAOTIP010000154 DAOTIP010000000
VERSION     DAOTIP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35357_length_3223_cov_3.213068"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..575)
                     /locus_tag="P5138_03920"
     CDS             complement(216..575)
                     /locus_tag="P5138_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59751.1"
                     /translation="MVDRGRGLQRASDYLDCIPTKESTVTAKIGPYKLDQVRFSPDRD
                     ELDLWTGDSGLAIMDCETSDGDRWFVPANESTEFTGIEIERVKARAQGGLTVELPTGE
                     RVRVEGIDSIIGAPGSD"
     gene            complement(850..1266)
                     /locus_tag="P5138_03925"
     CDS             complement(850..1266)
                     /locus_tag="P5138_03925"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRV59752.1"
                     /translation="MVLPLLLITLLVASVTDLRSREIPNWLMAAAALAGIALAAVEGR
                     AGSAVLFGLLASLPFLAAALIRPEGMGMGDVKLVAVIGLYLGPAVWIALAAGLALAGL
                     TGVLIALGRRLPPSKTALPLAPFLTAGCVAVLGFTL"
     gene            complement(1456..2559)
                     /locus_tag="P5138_03930"
     CDS             complement(1456..2559)
                     /locus_tag="P5138_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV59753.1"
                     /translation="MLVAELQELEEVKTLVNRGQQLGVLTFGDVATAVAEVDLDESDV
                     EDLYGHIEKQGIELVEDVDPAQKTSAENERSDGRRGRRRKKEALDLKPDMTTDSLQLF
                     LKDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDL
                     IQEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNK
                     IGRAERKLVTELGREPTPEEIAEVTGIDPEEVDSIKRSAQSPVSLEKPVGDEEESEFG
                     QFIADEKAESPFDRAADLLTKEALKESLENLSYRERRVLELRYGLGGEHPRTLDEVGR
                     TFNVTRERIRQIENQSLKKLQSLNEAQKLREAT"
     gene            2841..>3223
                     /locus_tag="P5138_03935"
     CDS             2841..>3223
                     /locus_tag="P5138_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRV59754.1"
                     /translation="MAHPNEAPIPLSEQWRHLHWVQAFWTRAWRENVTGLSGMVAYNL
                     MLAIFPFAFLVLFIFSQVLQINGVESGIISDLQRLFPNAEHGTLDDVLRAIRNNSATL
                     GVAAVIGSLWIGASFWGAMDTAFCR"
BASE COUNT          591 a          977 c         1006 g          649 t
ORIGIN      
        1 tttcaccggc gaggtcccgg atcacgagga gggtccactt gccggagatc acgtctgcgg
       61 tgcgacatac agggcactga tcgttgtcga tggcgctcat ggtgtgaagt gaccggttcg
      121 attgcggccg ggcggcgcgt acggcgcccc tgaccaacag gtcacttcaa caagtatagg
      181 cacaaggctt catttactga agtgcatggg tgggttcagt cgctgccggg ggctccgatg
      241 atgctgtcga tgccctcgac tcgcactctc tcaccggtcg gcagttccac agtcaggccc
      301 ccttgggccc tggccttgac tcgttcgatc tcgataccgg tgaattccgt tgattcattg
      361 gcggggacga accaacggtc accgtcgctc gtttcacaat ccatgattgc cagaccggaa
      421 tctccggtcc acaggtcgag ttcatcccga tccgggctga aacgaacctg atcaagtttg
      481 tacggaccga ttttggccgt cacggtgctt tcctttgtgg gtatgcagtc cagataatcg
      541 ctggcccgtt gtagtccacg accacggtca accaagccga cgaacccaga ccttttcgat
      601 ggtactcgat gcgatttgga tgactttttg atcgattcca gagatcggga ccgcgcactg
      661 tcatcaggat ttcgtggacc gctggatcac ggccaggctt tgaccggatg taccgtaagt
      721 ctgaaggcca aagcaacacc ctgcgatctg cgggatcgac acatatgccg agtggcccgt
      781 cggcatccac aatcgacctt ggccgctccc atggtcgtgg tttcccctct tcgaaacctt
      841 gagactcgct cacagcgtga atccaagaac tgccacgcag ccggcagtca ggaacggggc
      901 cagcggcagg gctgtcttcg acggagggag gcgacgaccg agggcgatca ggactccggt
      961 gaggccggcg agggcgagac ctgcggcgag ggcgatccag accgccgggc cgaggtagag
     1021 gccgatcact gcgaccagtt tgacgtcgcc catgcccatc ccctccggtc tgatcagggc
     1081 cgcggccagg aacggaagcg aggccagcag cccgaatagc acggcggaac cggcccggcc
     1141 ttctacggcg gcgagggcaa tcccggccag ggccgccgcg gccatcaacc agttcgggat
     1201 ctcgcgactg cgaaggtcgg tcaccgaggc gaccaggagg gtgatcagga ggagaggcag
     1261 gaccatgccc cgaccctgcc cgaaaaaagc tcacagatgg cactccgggt gcaaaatcac
     1321 tgcaacagaa atgacctgag tccgccaggg cggggctgac gaggatttcc acagaaaagg
     1381 ccggcgatag tacccgtgag tacgtgagcc ggccttttct gtggtactga cgaagtcagc
     1441 gtcgtcctgg tggactcagg tggcttcgcg gagcttctgg gcctcattga ggctctggag
     1501 cttcttcagc gactggttct cgatctggcg gatccgctcg cgggtcacgt tgaaggtgcg
     1561 cccgacctcg tcgagggtgc ggggatgctc gccaccgagt ccgtaacgga gctcgagcac
     1621 gcggcgctcg cggtaggaga ggttctccag cgattccttc agcgcttcct tggtcagcag
     1681 gtcggcggcc cggtcaaagg gcgactcggc cttctcgtcg gcgatgaact ggccgaactc
     1741 ggattcctcc tcgtcaccga ccggcttctc gagcgagacc ggcgactggg cggaacgctt
     1801 gatcgagtcg acttcctcgg ggtcgatgcc ggtgacttcg gcgatctcct ccggggtggg
     1861 ctcgcggccc agctcggtga ccagcttgcg ctcggcgcgg ccgatcttgt tcagcttctc
     1921 gacgacgtgc accgggatgc ggatggtccg ggccttgtcg gccagggcgc gggcgatcgc
     1981 ctggcggatc caccaggtcg cgtaggtcga gaacttgaac cccttgcggt agtcgaactt
     2041 ctcggccgca cggaccaggc cgagggtgcc ttcctgaatc aggtcgagga agggcaggcc
     2101 ctggttgcgg tagttcttgg cgatcgagac gaccagccgg aggttcgact cgaccatctt
     2161 ctgcttggcg tcgaggtcac cgcgctcgat ccgcttggcg agatcgactt cttcctgggc
     2221 agtcagcagg cgaaccttgc cgatgtcctt gaggaagagc tgcagcgagt cggtggtcat
     2281 gtccggcttg aggtcgagtg cctccttctt gcgccggcgg ccacgacgac cgtcggagcg
     2341 ctcgttctcg gcggaagtct tctgggcggg atccacgtcc tcgaccagct cgatcccctg
     2401 cttctcgatg tggccgtaga gatcttcgac atcggattcg tcgagatcga cctcggccac
     2461 ggcggtggcg acatcaccga aggtgagcac gccgagctgc tgcccacggt tgacgagggt
     2521 ctttacttct tctagttcct ggagttcggc tacaagcatc ttgtccctat ctggtattcc
     2581 ctgaggcggc acctaaacct gtcaacccga aaatcgaggg ttcggatgac agtgaaattg
     2641 cgaatcactc gtcaggaccc gcatcgctcc tttttttctg tcggagcagg aaaaaccgcc
     2701 gccgtctgta aatggtcaaa cgagtgatcg gataatagtc gcttcactta gtaaagtcaa
     2761 ggggctgtaa tccttgaaca cccggtactc aaggaactcc atccggcccc atgagttgca
     2821 ctgactgtga cgaaaatcaa atggcgcacc cgaacgaagc gccgattccg ctgtccgaac
     2881 agtggcggca tctccattgg gttcaggcct tctggaccag ggcctggcgc gaaaacgtaa
     2941 ccggactctc gggcatggtc gcctacaacc tgatgctggc gattttcccc ttcgcctttc
     3001 tggtcctgtt cattttctcg caggtgctcc agatcaacgg ggtcgagagc ggcatcatca
     3061 gcgaccttca acggctcttc cccaacgccg agcacggcac gctcgacgac gtcctgaggg
     3121 cgattcgcaa caactccgcc accctgggtg tcgcggcggt aatcggctcg ctctggatcg
     3181 gtgcctcgtt ctggggggcg atggacacgg ctttctgccg gat
//
LOCUS       DAOTIP010000155         3218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_35462_length_3218_cov_3.102118, whole genome shotgun sequence.
ACCESSION   DAOTIP010000155 DAOTIP010000000
VERSION     DAOTIP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35462_length_3218_cov_3.102118"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..227
                     /gene="rrf"
                     /locus_tag="P5138_03940"
     rRNA            109..227
                     /gene="rrf"
                     /locus_tag="P5138_03940"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            279..1463
                     /locus_tag="P5138_03945"
     CDS             279..1463
                     /locus_tag="P5138_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRV59755.1"
                     /translation="MPPEGEHQDSEATSNQIPVDTGLIEALSSGIGPRPPTSNSEHVA
                     AEVIASELSGMGLEARVESFCSQRSFGPTYLTLFALALAAGPLARKSRLAGALCGVAA
                     ASLGVAESSFASRSPLNFLRRGHSQNVWARIEPAEKSEQTICLVSHMDSSRSGLMFHP
                     SVTPHLGKAVQISGVAVAVNALAPVLRLAAPGRWLVRLSRILIGASAALVLERELRGE
                     NVAGANDNASGVAACLNLAAHFSRSPLRRSRLAVLVTGSEESGVIGMREFLRSHDTSG
                     WLFVNFDGVSADAPLRVLSREAGPAGSKPDPGLMEAARKVGERDPELAAEPLLDGSGL
                     PYDATAVMAGGGKAITIANQEGAILNYHWPTDTAEHIAPEAFARAVRFAAALVEELDS
                     VE"
     gene            complement(1528..1998)
                     /locus_tag="P5138_03950"
     CDS             complement(1528..1998)
                     /locus_tag="P5138_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59756.1"
                     /translation="MQKEKKSSRVAARAADAIDLMIEFATLGEYGLEYPEPVRSTSRR
                     KSTPHTPMNQAEGNDGPHHHGGGESASTTGHRHGAGPQRHRHGRRPCRPGRTRVDLPG
                     WSERPGHEVPGVQPGRRHKAEVRSITEPRKHQRNRPTSALPEALRPPRIRLEAA"
     gene            complement(2268..>3218)
                     /locus_tag="P5138_03955"
     CDS             complement(2268..>3218)
                     /locus_tag="P5138_03955"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRV59757.1"
                     /translation="YGPEYLASLLNEQPMGFYPPDSLVQEARRTGIEVLPPDVNRSRV
                     QCVTEWPQEAEEDPEPGEAAGCDGQPAVRIGLGYVKSINESEMEKLVAERDRAGAFGN
                     LGELSARLPLARTDLEQLAWAGALRSLPRGDRVAGLWNVGLAPGRRPMTGGRQLALPF
                     DPESTPALDEPRDWSRVQAEYSSIGMTLEGHPMELARRQVPETVLPTTDAAARPDRSW
                     VEVAGLQVARQRPETANGVIFILIEDEHGTLNLILPPPVAKRHRQVIRTATLIRAGGR
                     IETSQGVVNLLVRGLSEIVPPDPRIKAIAPVGFNFGRRGR"
BASE COUNT          511 a          985 c         1085 g          637 t
ORIGIN      
        1 cacctgtagc tgaccggtac taatgggtcg agggcttgac ggatatttat ttcaatcggt
       61 caatttgcgg ttttcaagct ttatcgaggc acatagtctc gacatatttc cggtggcgta
      121 atagcgtggt ggaaacacct cttcccattc cgaacagagc cgttaagcac cacagcgccg
      181 atggtacttg gggggcaacc ccctgggaga gtaggtcgcc gccggttctt cattagccgc
      241 ccttcggggc ggcttttctt ttgcagtact gttgcggcgt gccgcccgaa ggagaacatc
      301 aggattccga agcgacgagc aatcagatcc cggttgacac cggtctgatc gaggctctca
      361 gttccgggat cgggcccagg ccaccgacca gcaacagtga gcatgtggcc gccgaggtga
      421 ttgcctccga gctctcaggc atgggtctgg aagctcgcgt cgagtcgttt tgttcccaaa
      481 ggagcttcgg gcctacctac ctgacactct tcgcccttgc cctggccgcc gggccacttg
      541 cgcggaagtc gcgtctcgcc ggagccctgt gtggtgtggc cgccgcatcc ctgggggtgg
      601 cggagtcaag ctttgcgtcc aggagcccgc tcaacttcct gcgacgcggc cacagccaga
      661 acgtctgggc ccgtatcgag ccggctgaaa agtctgaaca gacaatctgc ctggtctcac
      721 acatggattc atcacgatcc ggcctcatgt tccacccctc cgtcactcct cacctgggta
      781 aggcggttca gatttcgggc gtcgctgtag cggtcaacgc cctggcgccg gtcttgaggc
      841 tggccgcccc gggcagatgg ctggtcaggc tgtcgaggat tctgatcggt gcttcagcag
      901 cactggtact ggaaagggag ttgaggggag agaacgtcgc cggggcgaac gacaatgcct
      961 cgggtgttgc cgcctgtcta aacctggctg cccacttctc gcggagtccg ctccgccgct
     1021 cccgcctggc agtgctggtg acgggcagcg aggaatcagg ggtgatcggg atgcgggaat
     1081 tcctccggag ccacgacaca agcgggtggc tcttcgtgaa tttcgacggg gtgagtgccg
     1141 acgcccccct tcgcgtccta tcgcgcgagg caggtccggc cgggtcgaag ccggatccgg
     1201 gattgatgga agctgccagg aaagtggggg agagggatcc tgaactggca gcggaaccgc
     1261 tgctggatgg atccgggttg ccctatgacg cgaccgccgt gatggccggg ggcggaaaag
     1321 cgatcacgat cgcaaatcag gaaggggcaa ttctcaacta ccactggccg accgacaccg
     1381 ccgagcacat cgcgccggag gccttcgccc gggcggtgag attcgcggcc gccctggtcg
     1441 aggaactgga ctcggtcgag tagtcgggct cggtcaggta gcccaatcgg gatgggcagg
     1501 tggactccgg atccggctga ccgcggctca ggctgcttcg agccggatcc ggggagggcg
     1561 tagcgcctcg ggcagggcag aagtggggcg gttgcgctgg tgcttccgtg gctccgtgat
     1621 cgaacggacc tcggccttgt gacgccggcc gggctgaacg ccgggtacct cgtgaccggg
     1681 acgctcactc cagcccggca ggtcgacacg ggtgcgaccg ggccggcagg gtcgacggcc
     1741 gtggcgatgt ctctgcggtc ctgcgccgtg acggtgacct gtcgtggatg cggattctcc
     1801 gccgccgtga tgatgaggac cgtcgttgcc ttctgcctgg ttcatcgggg tgtgtggggt
     1861 ggacttcctg cgactggtcg atcggaccgg ttcggggtac tcgaggccat actcgccgag
     1921 tgtcgcgaac tcgatcatca ggtcgatcgc atcagccgcc cgggctgcga cccggctgct
     1981 cttcttttct ttctgcacga acataggttc gtaatgtagg cacccggtcg gacacgaaca
     2041 catgtttgca aggttccgtg cagtttggtt cgcaaatccg cctgtagagc caagtgcaaa
     2101 ttgacattcg accgcctctc gcgctttctg cacgaacagg cgtttgtctt ctgttcgcat
     2161 tttgcaggat cttcccgggg cggtggggaa tctgttccag gggcatctgt ctcgtctgtt
     2221 caagtgattt tccgcgcctg tccgggtgat ctttcgcgcc ggccgggtca tctgccgcgt
     2281 ctgccgaagt tgaatccgac cggtgctatg gccttgattc gcgggtcggg gggaacgatc
     2341 tcgctcaagc cgcggaccag caggttgacc accccctggc tggtctcgat gcgaccgccg
     2401 gcccggatca gggtggcggt gcggatcacc tgccggtggc gtttcgctac cggcgggggc
     2461 aggatcaggt tgagggtgcc gtgctcgtcc tcgatcagta tgaagatcac gccgttggcg
     2521 gtctccggcc gctggcgagc gacctggagg cccgccactt ccacccagct gcgatccggc
     2581 cgggctgccg catcggtggt tggtagcacg gtctcgggga cctgacggcg cgcgagttcc
     2641 atcgggtggc cctcgagagt cattccgatc gagctgtatt cggcctggac acgggaccag
     2701 tcacggggtt catccagggc cggagtggat tcggggtcga agggaagggc gagctgccgg
     2761 cctccggtca tcggtcggcg acccggagcc agcccgacgt tccacaatcc cgcgacccgg
     2821 tcgccgcgcg gcagggaccg gagcgcgccg gcccaggcca gttgctcgag gtcggtccgg
     2881 gcgagtggca ggcgggcaga aagctcaccg agattcccga aggcaccggc gcgatcgcgt
     2941 tcggcaacca gtttctccat ttcactctcg ttgatggatt tgacgtagcc caggccgatt
     3001 cgtaccgccg gctgtccgtc acacccggcc gcttcgcctg gctctggatc ctcttcggcc
     3061 tcctgtggcc actcggtgac gcactggacc cggctgcggt tcacatccgg gggcagcact
     3121 tctatgccgg ttcgccgggc ctcctgcacc agcgaatcgg ggggatagaa gcccatcggc
     3181 tgctcgttca ggagcgaagc caggtactcc ggaccgta
//
LOCUS       DAOTIP010000156         3193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_35808_length_3193_cov_2.449968, whole genome shotgun sequence.
ACCESSION   DAOTIP010000156 DAOTIP010000000
VERSION     DAOTIP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35808_length_3193_cov_2.449968"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="P5138_03960"
     CDS             complement(<1..976)
                     /locus_tag="P5138_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRV59758.1"
                     /translation="MSAMEKVSIGITGETPEDMAAMARLAEAAGFDCGWAIELFRNAF
                     TQTTFLGAKTESMGIGTAIAWGFVRSPMTMAMSAMDIDEITGGRFRLGLGPGVKRLNE
                     TWHNAEYGRPAPHLRETIESVRAIIHAAAASEPVAYKGEYYDLDIKGWFRSTPPVRAD
                     IPIYTAAVQKGMSRMAGDVADGLIGHIICSARWIEQEIVPAFELGLSRSGRDRSSFEF
                     LPSICVAIDDDYQRGLEAARKTLAFYATVKTYLPLFELHDFGQNAADAGAAFRMGDLA
                     GVAEAISDEMVETYCAVGTPDKVKEKVAEVAQFGDAILPLLAIFHAAHD"
     gene            1098..2867
                     /locus_tag="P5138_03965"
     CDS             1098..2867
                     /locus_tag="P5138_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59759.1"
                     /translation="MKLAGLAVMAVAFFGSTAAASADPVVFNFDESIINLGGGSGPQG
                     LKLIDPDADPADAPATLNGEVDTATDAFTAAASGFTFPQKTTQVSGQDVVVTITANAA
                     ITGTFDTDTGTADITVPVTVSAAVLGNTCTTSFPLELKTTGTLVEPGGNHDAAPFDPD
                     TGEGSVYSSFTVPNSTGGALCGVVDGQIGGAGDIWLSGTGGIDEGIVTVYPGDDGTSL
                     STTDGGWVHSTSYGPLCALIPGVLCPAMNGTFESANGSSGAADGFLRDTATGLTVTPT
                     GLGQTSTGVWTSPEFTYDGAEGEPAETLSFALARKGTLSDLLSQSSANWQVDLVKSSD
                     SSIIPLIPNGPATNADSWVNIPGSALAADGLTIGDAYRIVITTNVITETLGLAPSGTF
                     DYDEVALTARRATPTGPTGPTGPTEPTGPTGPTEPTGPTGPTEPTGPTGPTSPTGPTG
                     PTGPTGPNKPVTLTKGNAAFNGKRLFIRLYCPREFKPKCNTVSVPVTRKVANKAPKKI
                     KRLSKPMARPIRVTVKSGKWKKVSYLIKPAYRNKVRKMAGKNKRLLAIRDYIKAKKFA
                     GRKFKGKTRTVFHSHKVVIGKAR"
     gene            complement(2955..>3193)
                     /locus_tag="P5138_03970"
     CDS             complement(2955..>3193)
                     /locus_tag="P5138_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HRV59760.1"
                     /translation="INEAFASVVKAWEIELQPDMDKVNVNGGAIALGHPLGSTGARLM
                     TTLLHELERQDKKTGLVTMCCGGGIGTATLIERI"
BASE COUNT          608 a         1087 c          963 g          535 t
ORIGIN      
        1 ggtcgtgggc ggcatgaaaa atcgccagga gcggcaggat cgcgtcgccg aactgggcga
       61 cctcggccac cttttccttc actttgtccg gggtgccaac cgcgcaatag gtctcgacca
      121 tctcgtccga aatcgcttcg gccactccgg cgaggtcgcc catgcggaag gccgcaccgg
      181 catcggcggc gttctgaccg aagtcgtgga gttcgaagag cggcagatag gtcttgactg
      241 tcgcgtagaa agccagggtc ttgcgggcag cttcgagacc gcgttgatag tcgtcgtcga
      301 ttgccacgca gatgctcggc aggaactcga aggaagaacg atccctcccc gagcgggaaa
      361 gaccgagttc gaaggccggg acgatctcct gctcgatcca tcgggccgag cagatgatgt
      421 ggccgatcag accgtcggcg acgtcaccgg ccatgcgcga catgcccttc tgtaccgccg
      481 ccgtgtagat cgggatgtcg gcgcggacgg gcggggtcga tcggaaccag cccttgatgt
      541 cgaggtcgta gtactcgccc ttgtaggcaa ccggctcact ggccgctgcg gcgtggatga
      601 tcgcccggac tgattcgatc gtttcccgta gatgcggggc aggccggccg tactcggcgt
      661 tgtgccaggt ctcgttcagc cgtttgacgc ccgggccgag ccccagccgg aagcgcccgc
      721 cggtgatctc atcgatgtcc atcgcactca tcgccatggt catcgggctg cgcacgaacc
      781 cccaggcgat cgcggtcccg atgcccatcg attcggtttt cgcaccgagg aatgtcgtct
      841 gggtgaatgc attgcggaat agttcgatcg cccagccgca gtcgaaaccg gccgcttcag
      901 ccagccgggc catggccgcc atgtcctccg gcgtctcccc cgtgatcccg atcgaaacct
      961 tctccatagc gctcatgctc ctgagcgtat ctgtttgaaa tcccgaccag gtggtagtgg
     1021 gtcattgcgt acccgtccgt acgcaagtgg agagaaaccc aggcaccaag ggaaaggcaa
     1081 gcatgaacag gatgttgatg aaactggcag gactggccgt catggcggtc gcttttttcg
     1141 gctcgaccgc tgccgcgtcg gcagatccgg tcgttttcaa tttcgatgaa tcgatcatca
     1201 atctcggagg cggctcgggc ccgcaaggcc tcaagctgat cgaccccgat gcggaccccg
     1261 ccgatgcccc ggccaccctg aatggcgagg tggacacggc aactgacgct ttcaccgccg
     1321 cagcatccgg cttcaccttt ccccagaaga ccacccaggt ttcaggtcag gacgtcgtcg
     1381 tgacgatcac cgccaacgcg gcgatcaccg gaacgtttga caccgacacc ggcaccgcgg
     1441 acataacggt cccggtcacc gtcagcgctg ccgtactcgg caatacctgc acgacctcct
     1501 tcccgctcga actcaagacc acgggcactc tggtcgaacc cggcggtaac catgacgcgg
     1561 ccccctttga ccccgacacc ggtgagggga gcgtctacag ctcgttcacg gtgccgaact
     1621 cgaccggtgg cgccctctgc ggcgtggtcg acggccagat cggcggcgcc ggtgacatct
     1681 ggctctccgg cactggcgga atcgatgaag gcatcgtcac cgtctatccc ggcgatgacg
     1741 gcaccagcct ctccaccacc gacggcggct gggtccacag cacctcgtac ggcccgctct
     1801 gcgcactcat cccgggagtt ctctgccccg cgatgaacgg gaccttcgaa tcagccaacg
     1861 gcagctccgg ggccgccgac ggcttcctgc gcgacacggc caccggcctg acagtcaccc
     1921 cgaccggcct cggccagacc tcgaccggag tctggaccag ccccgagttc acctatgacg
     1981 gggccgaagg cgaaccggcc gagactctga gttttgccct ggccaggaaa ggcaccctct
     2041 ccgacctgct cagtcagtcc agcgccaact ggcaggtcga cctggtcaag tccagcgaca
     2101 gttcgatcat cccgctgatt ccgaacggtc ccgcaaccaa tgccgattcc tgggtgaaca
     2161 tcccgggcag cgcgctggcg gctgacggcc tgacgattgg cgacgcgtat cgcatcgtga
     2221 tcaccaccaa cgtgatcacc gagacgctcg gcctggcccc ctctggaacc ttcgactacg
     2281 acgaggtggc gctcacggcc cgtcgcgcaa ccccgaccgg tccgacgggc cccactggcc
     2341 cgaccgaacc cacaggtcct actggcccga cggaaccgac cggtcctacc ggccccaccg
     2401 agccaaccgg cccgaccggt ccgacttccc cgaccggccc caccggcccc acgggaccga
     2461 ccggaccgaa caagccggtc accctgacca agggcaacgc cgccttcaac ggcaagcgac
     2521 tgttcattcg cctgtattgc ccacgcgagt tcaagccgaa gtgcaacacg gtttcggtgc
     2581 cggtgaccag gaaggtcgca aataaggccc cgaagaagat caagcgcctg tcgaagccga
     2641 tggccaggcc gatccgggtg acggtcaagt cgggcaagtg gaagaaggtc tcctacctga
     2701 tcaagcctgc ctaccggaac aaggtcagga agatggccgg caagaacaaa cggctgctgg
     2761 cgatccgcga ctacatcaag gcgaagaagt tcgccggccg gaagttcaag ggcaagaccc
     2821 gaacggtctt ccacagccac aaggtcgtga tcggcaaggc cagataacga acctccctcc
     2881 caaatgaagt gccctccaaa tgaaagggcc cgggtctctg gacaagaccc gggccctttc
     2941 gccttctgac ggactcagat tcgttcgatg agcgtggcgg ttcctatccc gccgccgcag
     3001 cacatggtga ccagaccggt cttcttgtcc tgacgctcga gctcatggag cagggtggtc
     3061 atcaggcggg cgccggtcga gccgagcggg tggccgaggg cgatcgcgcc gccgttgaca
     3121 tttaccttgt ccatgtcggg ctgtagctcg atctcccagg ccttgaccac cgaggcgaag
     3181 gcctcattga tct
//
LOCUS       DAOTIP010000157         3191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_35837_length_3191_cov_2.538903, whole genome shotgun sequence.
ACCESSION   DAOTIP010000157 DAOTIP010000000
VERSION     DAOTIP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35837_length_3191_cov_2.538903"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="P5138_03975"
     CDS             complement(<1..1260)
                     /locus_tag="P5138_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRV59761.1"
                     /translation="MTRSERILITGAREHNLKGIDLDLPRDALIVVTGLSGSGKSSLA
                     FDTVYAEGQRRYVESLSAYARQFLGLMEKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARIGHPHCPNCGKPITGQTQEQITDQLMTMEDGTRFMVMAPVVRG
                     RKGEYGKLFEQFRADGYSRVEVDGEMRMLDEEITLDKKYKHDISVVVDRLVMKTDLRR
                     RLSESVEAAASMAEGLVEIQEIDGERQTFSENFACLECGTSMPELEPRIFSFNAPHGA
                     CPACTGLGFKKVIDPELVVPDPTLSVAEGALQPWRSGYSGYWRRLNKSVAETYGIDID
                     TPWQELTQKERDVFLYGTGSERHTIRTRSRAGRQRSYQVKFEGLVNNLERRYQESDSE
                     AVREKIEGYMAEQPCPSCHGARLRPESL"
     gene            complement(1361..1831)
                     /locus_tag="P5138_03980"
     CDS             complement(1361..1831)
                     /locus_tag="P5138_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59762.1"
                     /translation="MEREHVVRDDFPRAHGSEGFDVDSVGAHLAAVAAHVAALEARIN
                     ALQVECETLRGQLADAREHEEGPSGLPEPGPDPLANLGGEEDKSPAETLPADPEPDDA
                     AGVAPGEDEAAARMLASKLAMGGTSRDTIVMQVAAEHEVANPGALVDDVLARLA"
     gene            complement(1856..>3191)
                     /locus_tag="P5138_03985"
     CDS             complement(1856..>3191)
                     /locus_tag="P5138_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV59763.1"
                     /translation="IHHFDPLTGEILDEVQHVSIWPATHYVTEEDSVERTLEAIRTEL
                     DQRLEELESEGKELEAHRLRQRTLYDLEMIKETGFTSGIENYSRLFEGRAEGTPPHTL
                     IDYFPDDFITFVDESHQTIPQIGGMYMGDRSRKQTLVDYGFRLPSAIDNRPLKFDEFM
                     ERMNQLVLVSATPGEFERTESNRVVEQIVRPTGIVDPEIEVRPTANQIDDLMNEIRAT
                     TDQGERTLVTTLTKKMAEDLTNYLIEHGVKVRYLHSDIDTLERIEIIRDLRLGEYDVL
                     VGVNLLREGLDMPEVSLVAILDADKEGFLRGETSLIQTIGRAARNTAGRVLMYGDKET
                     EAMRSAISETDRRRAIQVAYNERHQITPTTIVKGVAEEGNLLAIEDKAPSRRRRRQED
                     EFDGPDELEKTIVQMEEEMHEAAEDLRFEQAARLRDEVRELRRDLEAMKAGA"
BASE COUNT          495 a         1048 c         1005 g          643 t
ORIGIN      
        1 cagactctcc ggcctcaggc gagccccgtg gcagctgggg cagggctgct cggccatgta
       61 gccctcgatc ttctcgcgta ccgcctcgga gtcggactcc tgataccggc gctcgaggtt
      121 gttgaccagg ccttcgaact tgacctgata gctgcgctgg cggccggccc ggctcctggt
      181 gcggattgtg tgccgctcgc taccggtgcc gtacagaaag acatcccttt ccttctgcgt
      241 cagctcctgc cagggagtgt cgatgtcgat cccgtaggtc tcggccaccg acttgttcag
      301 ccgccgccag tagcccgaat agcccgagcg ccagggctga agggcaccct ctgctacgga
      361 gagggtcggg tccggaacga caagttccgg atcgatcacc ttcttgaaac cgaggcccgt
      421 gcaggccggg caggcaccgt gaggcgcgtt gaaggagaag atcctgggtt cgagttccgg
      481 catcgaggtg ccgcattcca ggcaggcgaa gttctccgag aaggtctggc gttccccgtc
      541 gatctcctgg atctcgacca ggccctcggc cattgaggct gccgcctcaa ccgattccga
      601 gagccgcctt ctgaggtcgg tcttcatgac caggcggtcg accacgaccg atatgtcgtg
      661 cttgtacttc ttgtcgaggg tgatttcctc gtcgagcatc cgcatctcgc cgtcgacttc
      721 gaccctcgaa tatccgtccg cccggaactg ctcgaagagc tttccgtact cgcccttgcg
      781 cccgcgaact accggcgcca tgaccataaa gcgggtgccg tcttccatgg tcatcaactg
      841 gtcggtgatc tgttcctggg tctggccggt gatcggcttg ccgcagttcg ggcaatgcgg
      901 atgtccgatc cgggcccaga gcagacgcag gtagtcgtag atctcggtca ccgttccgac
      961 cgtcgaacgg gggttgcgcg aagtggtctt ctggtcgatt gaaatcgccg gggaaagccc
     1021 ctcgatcgaa tccacgtccg gcttctccat cagcccgagg aactggcggg cataggccga
     1081 aagtgactcg acatatcgcc gctggccttc ggcgtagacg gtgtcaaagg ccaggctcga
     1141 cttgccggaa ccggacagtc cggtgacgac gatcagcgca tcgcggggaa ggtcaaggtc
     1201 aatgcccttg aggttgtgct cgcgggcccc ggttatcagg atgcgttcgg aacgggtcac
     1261 agacacccat catacgaaac cgaacacacg ttcgttcgcg ccggtcggtg caaatcaccc
     1321 ccgatatacg ggggcgactc gaaccgaggg ggcctgaaag tcaggccaga cgcgcaagga
     1381 cgtcgtcgac cagggcgccg gggttggcga cctcatgttc ggccgccacc tgcatcacga
     1441 tcgtgtcccg gctggtaccg cccatcgcca gctttgatgc gagcatgcgg gcagcagcct
     1501 cgtcctctcc gggagcgacc cccgccgcat cgtccggctc cgggtccgcg ggcagggtct
     1561 cggccggact cttgtcctct tcaccgccca ggttcgcgag cggatccggg ccgggctcgg
     1621 gcagtccgct cggcccttcc tcgtgttcgc gtgcgtcggc caactggccg cgaagcgttt
     1681 cgcattcgac ctgtagcgcg ttgatccggg cctccagcgc cgccacatgc gcggccaccg
     1741 ccgccagatg ggcgccgacc gagtcgacgt cgaaaccttc cgatccgtgg gcccgcggaa
     1801 agtcgtcccg aaccacatgc tctcgttcca ttggtccacc ctaccccccg gatgcctagg
     1861 ccccggcctt catcgcctcg agatccctgc ggagttcgcg tacttcgtcg cgcagccggg
     1921 ccgcctgttc gaaacggagg tcctcggccg cctcatgcat ctcctcttcc atctggacga
     1981 tcgtcttctc gagctcgtcc ggcccatcga actcgtcttc ctgccggcgc cttcggcggg
     2041 acggcgcctt gtcctcgatc gccagcaggt tgccttcctc ggctacgccc ttgacgatcg
     2101 tggtcggggt gatctggtga cgctcgttgt aggccacctg gatggcccgg cgacggtcgg
     2161 tctcggagat cgcggagcgc atcgcttcgg tctccttgtc cccgtacatg aggacgcgcc
     2221 ccgcagtgtt ccgggcggca cggccgatcg tctggatcag gctggtctcc ccgcgcagga
     2281 agccctcctt gtcggcgtcg aggatcgcga caagggaaac ctcgggcatg tccaggccct
     2341 cccgcaggag gttgaccccg accaggacgt cgtactcccc cagccggagg tcgcggatga
     2401 tctcgatccg ctccagcgtg tcgatgtccg agtgcaggta acggaccttt accccgtgct
     2461 cgatcagata gttggtcagg tcctcggcca ttttcttggt cagggttgtg accagcgtgc
     2521 gttcaccctg gtcggtggtg gcccgaatct cgttcatcag gtcgtcgatc tgattggcgg
     2581 tgggcctgac ctcgatctcg ggatcgacga tgccggtcgg acggacgatc tgctcgacca
     2641 cccggttcga ttccgtcctc tcgaactcgc ccggcgtggc cgaaacgagg acgagctggt
     2701 tcatccgctc catgaactcg tcgaacttga gcggccggtt gtcgatcgcg gagggcagcc
     2761 ggaagccgta gtcgaccagg gtctgcttgc gggagcggtc gcccatgtac atgccgccga
     2821 tctgcgggat cgtctggtgc gactcgtcga cgaaggtgat gaagtcgtcc gggaagtaat
     2881 cgatcagggt atgtggagga gttccctcgg cccggccttc gaacaacctc gagtagttct
     2941 cgatcccgga ggtgaaaccg gtctccttga tcatttccag gtcataaagc gtgcgttgcc
     3001 tcagccggtg ggcttccagc tccttgccct cggattccag ttcttcgagc cgctggtcga
     3061 gttccgtccg gatcgcctcc agggtccgtt cgaccgagtc ctcctcggtg acgtagtggg
     3121 tggccggcca gatcgacacg tgctgcacct cgtcgaggat ttcgccggtc aacgggtcga
     3181 agtggtggat c
//
LOCUS       DAOTIP010000158         3187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_35890_length_3187_cov_3.718072, whole genome shotgun sequence.
ACCESSION   DAOTIP010000158 DAOTIP010000000
VERSION     DAOTIP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35890_length_3187_cov_3.718072"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="P5138_03990"
     CDS             complement(<1..790)
                     /locus_tag="P5138_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRV59764.1"
                     /translation="MGNPGITVCGRLTVTWDGEQLEADLPGRQGRLIFAYLVLNRARP
                     VRRDELVEALWADEGLPSGGEALLAPPLSRLRKALGQGRLEGRAELSLNLGEEADIDY
                     ETAQRKLDFAHEVAGDRSVTGDRLAAGWEDAQEAARIFEGGLLPGLEARWIEEQRVYF
                     EELRLKSLETVARIGARLGPAEQAKAEKAARNAVEASPFRESARAALIELLEAEGNIA
                     EALRAYEELRVLLREELGTFPAPELGAIHERLLRAHESSGEKAEP"
     gene            complement(832..>3187)
                     /locus_tag="P5138_03995"
     CDS             complement(832..>3187)
                     /locus_tag="P5138_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59765.1"
                     /translation="SPRRNWISPAPGPAGDLESERRALFGAVMRMIGRLTARQPMLVV
                     LDDLHWADRSTLMLLASLAGDNPPGGVLALGIYRDTELPEDSLLPETLTNLQRRLPTL
                     KLEVEALDTEQVGQLVGNRLDATLATSLRDQTGGNPFLVEQLVRHLEETGIEDPEAAP
                     AEVRQIVSQRVNHLPDGGPDLLRRASLIGRDFDLPVLMATTSWDEDAVIDLLDAAVSA
                     GLLDESPTVPGRYSFVHALLRSTLEDELGLTRRAMIHRDIGEAIERQTASRPERRVGE
                     KAWHFIQAGPAETDRAVHWATQAAEQAEARLAYDEAVDFYEGAIAAARADEPVDQGLL
                     ARLLLSQANARWKNGSLQAAGDTFLEAAKAARESGLPELAAEAAIGSRWGGWDAFDAD
                     MAEQLNLMREALDGLPETDSPLRAELTAMLGNMLYYGSGKAAEARELADQAVEMIERL
                     DAPEAEFGVIQATAYRRWEPGRLDERLPASERMLELAEQLDDPELTGQGLVSRQLSLI
                     NLGRGAEAEADRSRHYAMSETLPQIRVISKAMRASAWFMTGDWQDGEELVRELETEGM
                     PRGAALLIEDALRFMVRAQQGALAEHLGRLELFEAGAKGWEMWPTWRLGLMLAKAQAG
                     QLDEVREMVAELGYDSLDETRDFNNTLLPFCAVGNLLAGELDDQEGAAKLIELLTPYS
                     GEWIIIARIGSTLGPVDMHLGELQLLAGNHREAATALERSLITCEVMEAVPYLARTRL
                     ALASAFEAMGDPDGAERRLRLRHEGEEVARRLDMQAILKRHLPA"
BASE COUNT          460 a         1102 c         1061 g          564 t
ORIGIN      
        1 ccggttcggc cttctcgcca gagctttcgt gtgccctgag aagccgttcg tgtatcgccc
       61 ccagttccgg agcggggaaa gttccgagtt cctcccgaag cagaacccga agctcctcat
      121 aggcgcggag cgcttcggcg atattgccct ccgcttcgag cagttcgatc agcgccgcgc
      181 gggccgactc gcggaagggc gaggcttcga cggcatttcg ggctgccttt tcggccttgg
      241 cctgttcggc cgggccgagc ctggctccga ttctggcgac cgtctcgagg gatttcaggc
      301 gaagttcctc gaaatagacc ctttgttcct cgatccagcg ggcctccagg ccggggagca
      361 acccgccctc gaagatacgg gccgcctcct gggcgtcttc ccagccggcc gcaagccggt
      421 cgccggtgac cgaacggtcg cccgccacct catgggcgaa gtcgagtttc cgctgcgcgg
      481 tctcatagtc gatgtcggct tcctcgccga ggttgagcga cagctcggcg cggccttcca
      541 gccggccctg cccgagggcc tttctcagcc tcgacagggg aggagcgagc agcgcttccc
      601 cgccgctggg caggccctcg tccgcccaga gggcctcgac cagttcgtcc cgtctcaccg
      661 gacgggcccg gttcagcacc aggtaggcga agatcagtct tccctggcgg cccggaaggt
      721 cagcctccag ctgctcgccg tcccacgtga cggtgagcct gccacagacc gttatcccgg
      781 gatttcccac cgcggcaact tatccgaacg gaccgtccga cgcgaacggg atcaggcggg
      841 aagatgccgc ttgagaatgg cctgcatgtc gagccttctc gcgacttcct cgccttcatg
      901 ccggagccgc aatctgcgtt cggcgccgtc ggggtcaccc atcgcttcga aggccgaggc
      961 gagggcgagc cgggtgcggg ccaggtaggg gaccgcttcc attacttcgc aggtgatcag
     1021 ggaacgctcg agtgcggtcg cggcctcgcg atggttgcct gccagcagct gaagttcccc
     1081 gaggtgcatg tcgaccggcc cgagggtcga gccgatccgg gcgatgatga tccactcgcc
     1141 gctgtacggg gtgagcagct cgatcagttt cgcggcgcct tcctggtcgt cgagctctcc
     1201 ggcaagcagg tttccgaccg cacagaacgg cagcagggtg ttgttgaagt ccctggtctc
     1261 atcgaggctg tcgtagccca gctcggcgac catttcccgc acctcgtcca gctggcccgc
     1321 ctgggccttg gcgagcatca ggcccaggcg ccaggtcggc cacatctccc agccctttgc
     1381 cccggcctcg aacagttcga gccgacccag gtgttccgcg agggcaccct gctgggcccg
     1441 caccatgaag cgcagggcgt cctcaatcag cagggcggcc ccgcgtggca tgccctcggt
     1501 ctccagttcc cggaccagct cctcgccgtc ctgccagtcg ccggtcatga accaggcgga
     1561 agcacgcatc gccttcgaaa tcaccctgat ctgggggagg gtctcgctca tcgcatagtg
     1621 gcggctgcgg tccgcctcgg cctcggcgcc gcgacccagg ttgatcagcg aaagctggcg
     1681 cgagacgagt ccctgaccgg tcagctccgg gtcatcgagt tgttccgcca gttccagcat
     1741 ccgctccgag gcggggagcc gttcatccag cctgcccggc tcccagcgcc ggtaggcggt
     1801 cgcctggatc actccgaatt cggcttcggg ggcgtcgaga cgctcgatca tctctaccgc
     1861 ctgatcggcc agttcgcgcg cttcggcggc cttgccactg ccgtaataga gcatgttccc
     1921 gagcatcgcc gtcaactccg cgcgaagggg agagtcggtc tcgggcaggc cgtcaagcgc
     1981 ttcgcgcatc agattgagct gttcggccat atcggcgtcg aaggcgtccc agccgcccca
     2041 ccggctgccg atcgccgctt ctgcggccag ctccggcagg ccggattcgc gtgccgcctt
     2101 ggctgcctcg aggaaggtgt cgcccgccgc ctgaagcgag ccgttcttcc accgcgcgtt
     2161 ggcctgactc agcagcagcc tcgccagcag gccctggtcg acgggttcgt cggccctggc
     2221 ggcggcgatg gcgccttcat agaagtcgac cgcctcgtcg taggcaagcc tcgcttcggc
     2281 ctgttcggcg gcctgggtgg cccagtgaac ggctcggtcg gtctccgccg ggcccgcctg
     2341 gatgaaatgc caggccttct cgccgacccg gcgctcggga cggctcgcgg tctgcctttc
     2401 gatcgcttcg ccgatgtccc ggtggatcat cgccctgcgg gtcagcccga gctcgtcttc
     2461 aagggtgctg cggagcaggg cgtgaacgaa cgaataccgc cccggaacgg tcggtgattc
     2521 gtccagcagg ccggcgctca cggcggcgtc gagcaggtcg atgactgcgt cctcgtccca
     2581 gctggtggtg gccatcagga ccggaaggtc aaagtcccgg ccgatcaggg atgcgcgcct
     2641 gagcaggtcg gggccaccgt cgggcaggtg attgacccgc tgggagacga tctggcggac
     2701 ctcggccggc gcggcctccg ggtcttcgat tccggtctct tcaaggtgac ggacgagctg
     2761 ttcgacaagg aaagggttgc cgccggtctg gtcacgcagc gaggtggcga gcgtcgcatc
     2821 gaggcggttt cccacaagct gcccgacctg ttccgtgtcg agagcctcga cctccagttt
     2881 cagggtgggc agacggcgct ggaggttggt cagcgtttcc ggcagcaggc tgtcctccgg
     2941 caactccgtg tcccggtaga tgccgagggc caggactccg cctggcgggt tgtccccggc
     3001 caggctggcc agcagcatca gggtcgaacg gtccgcccag tggaggtcgt ccagcacgac
     3061 aagcatcggc tgcctggcgg tcaggcgccc gatcatccgc attaccgcac cgaacagggc
     3121 ccggcgttcc gattcgaggt caccggccgg tcccggcgcg ggcgaaatcc agttgcgccg
     3181 tgggctc
//
LOCUS       DAOTIP010000159         3178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_36022_length_3178_cov_2.858149, whole genome shotgun sequence.
ACCESSION   DAOTIP010000159 DAOTIP010000000
VERSION     DAOTIP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36022_length_3178_cov_2.858149"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="P5138_04000"
     CDS             complement(<1..652)
                     /locus_tag="P5138_04000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRV59766.1"
                     /translation="MTPYYDLLNKPCGGTFDDIIDSIGHTPLVELKTLSPKEDVRIFA
                     KLESANPTGSVKDRVARAMMQSAEESGAIAPGQTILEPTSGNTGISLAMICRRRGYPL
                     KVVMPDNVTEERSQLLAMYGAEIVYSEGAKGSNGAVELALEMAEDPQYFMPYQYGNEA
                     NPNAHYDGTAVEILEELDEVTAFVAGLGTGGTLMGNGRRLKEANPETKLVAAEPMQG"
     gene            787..1644
                     /locus_tag="P5138_04005"
     CDS             787..1644
                     /locus_tag="P5138_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV59767.1"
                     /translation="MKKSRLIALLSALMILAAMVVVVGCGSSDDSSSGSGDFQSELIT
                     EGTLTVGSDIPYAPFEMGQAPDYTGFDIELVDDIANRLDLDVKIEDTAFDTIFTDVAQ
                     GKFDMVASAATITPEREQQVNFSDPYFESEQALLVPPGSDITSVEDLSGKTVAAQDGT
                     TGEAYANDETDAGQVRGFPTGAAAIAAVVNGQADAAIIDAPVAEDAASKGQAGFEIAT
                     HIPTGEYYGFAFAKDTPELLKAVNGALSDLIDDGTYEKLFKKYFNATPPASITDGTAA
                     ADTGTTDQG"
     gene            1739..2479
                     /locus_tag="P5138_04010"
     CDS             1739..2479
                     /locus_tag="P5138_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRV59768.1"
                     /translation="MDVFFDTFFNFQFMADHFQYVLDGFWVTIKLSLVGGICALVWGL
                     ILAVLRQLPGRALAPVRAVVIGYIDVFRGIPLLLVILLLSSGLATMATQGAIPREIGI
                     PEWFGESDPFWYGVMALTLTYGAYMAEVYRAGIEAVPQGQTEAARSLGMSHGQAMRFV
                     IIPQAVRKVIPPLLNDYIALMKDTALVSVIGLVEAVQAGRDLYSEFYVPSGYTLGAIF
                     FLIVTIPLARVVDWQIKKQQEKFQRGMP"
     gene            2476..>3178
                     /locus_tag="P5138_04015"
     CDS             2476..>3178
                     /locus_tag="P5138_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRV59769.1"
                     /translation="MSDEVTTLKREVARGETIMTVSNLKKSYGSLEVLRDIDLDIKKG
                     EVICVLGPSGSGKSTMLRCLNLLELPTGGTIALKGETVFDGGKPKNLDDLRRETGMVF
                     QSFNLFPNMSAKQNVSIAQVRVRGRKKDEADKRSVDQLCKVGLDEKVDEYPDRLSGGQ
                     QQRVAIARALAMDPEIMLFDEVTSALDPELVKGVLDVMRELADSGMTMICVTHEIGFA
                     REVADRVIFMDGGYIV"
BASE COUNT          567 a          990 c         1004 g          617 t
ORIGIN      
        1 cgccctgcat cggctcggcc gcgaccagct tggtctccgg gttggcttcc ttgagccggc
       61 ggccgttgcc catcagggtg ccaccggtgc cgaggccggc cacgaaggcg gtaacttcgt
      121 cgagttcctc gaggatctcg accgcggtgc cgtcgtaatg ggcgttcggg ttggcttcgt
      181 tgccgtactg gtagggcatg aagtactgcg ggtcctcggc catttccagg gccagctcca
      241 ctgcgccgtt ggatcccttg gcaccttcgg aatagacgat ctcggcgccg tacatggcga
      301 gcagttggct ccgctcctcg gtcacgttgt cgggcatcac caccttgagc ggatagccgc
      361 ggcggcggca gatcatcgcc agggagatac cggtgttgcc cgaagtgggc tcgaggatgg
      421 tctggcccgg tgcgatcgcg ccggactcct cggccgactg catcatcgcg cgggcgaccc
      481 ggtccttgac cgacccggtc ggattggccg actcgagctt ggcgaagatc cgcacatcct
      541 ctttcgggga aagcgtcttc agctcgacca gcggggtatg accgatcgaa tcgatgatgt
      601 catcgaatgt ccccccgcag ggcttgttca gaaggtcgta gtaaggagtc attttcgtgg
      661 ggctgctgga ccgcggtact tcattaagtg tagtcgcttg gacattcgtc ggaagatcgt
      721 ggccggaatc gtgaaatact ggcggggttg cgtccagcat tcaatccctt cctggaggaa
      781 acacgcatga agaaatcccg tttgatcgcg ctgctttcgg cgctgatgat tctcgcggcg
      841 atggtggttg tcgtcggctg tggcagcagt gatgacagca gcagcggcag cggcgacttc
      901 cagtccgagc tgatcaccga aggaaccctg acggtcggct ccgacatccc gtacgcaccg
      961 ttcgagatgg gtcaggcccc cgactacacc ggtttcgaca tcgaactggt cgatgacatt
     1021 gccaatcgtc tcgacctcga cgtcaagatc gaggacaccg ccttcgacac catcttcacc
     1081 gatgttgccc agggcaagtt cgacatggtc gcttcggctg ccacgatcac gcctgaacgc
     1141 gagcagcagg tgaacttctc ggatccgtac ttcgagtccg agcaggccct tctggtgccc
     1201 ccgggttcgg acatcacctc ggttgaggac ctgtccggca agaccgttgc cgcccaggac
     1261 ggaaccaccg gtgaggctta cgccaacgac gagaccgacg ctggccaggt ccggggcttc
     1321 ccgaccggcg ccgccgcgat cgccgctgtc gtgaacggcc aggccgacgc cgccatcatc
     1381 gatgctcctg ttgccgagga cgctgcgtcc aagggtcagg ccggcttcga gatcgcgacc
     1441 cacatcccga ccggcgagta ctacggcttc gcattcgcga aggacacgcc ggaacttctg
     1501 aaggcagtga atggcgccct ctccgatctg atcgacgatg gcacctacga gaagctgttc
     1561 aagaagtact tcaacgcgac gccgccagcc agcatcactg acggcacagc ggccgctgac
     1621 accggaacga ctgaccaggg ttagtttccg gatcgcttac agagagggcg ccccgcgggg
     1681 cgccctctct gtttcgcttt cccggcgtga cccgcgccgg ggctgactga aagaagccat
     1741 ggacgttttt ttcgacactt ttttcaactt ccagttcatg gcggaccact tccagtacgt
     1801 actggacggg ttctgggtaa ccatcaagct ctcccttgta ggcggcatct gcgcgctggt
     1861 ctggggactg atcctggccg tactgcggca gctgccaggc agagcgctgg ctccggttcg
     1921 tgctgtggtg atcggttaca tcgatgtctt ccgcggtatc ccgctgctgc tggtcatcct
     1981 gctcctttcg agcggactcg ccaccatggc gacgcagggg gccattcccc gcgagatcgg
     2041 catccccgaa tggttcggcg agtccgatcc gttctggtac ggcgtgatgg ccctgaccct
     2101 gacctacggc gcctacatgg ccgaggtcta ccgggccggg atcgaggccg tgccccaggg
     2161 gcaaaccgag gcggcccgct ccctgggcat gtcccacggc caggccatgc gcttcgtgat
     2221 catcccgcag gcggtgcgca aggtgattcc gccgctgctc aatgactaca tcgccttgat
     2281 gaaggacacc gccctggtca gcgtcatcgg tctggtcgaa gccgtccagg cgggccggga
     2341 cctctactcg gagttctacg ttccctccgg ctacacgctt ggggcgatct tcttcctgat
     2401 cgtcaccatc ccgctcgccc gcgtggtcga ctggcagatc aagaaacagc aggagaaatt
     2461 ccagagggga atgccgtgag cgacgaggta acaactctca agagggaggt cgcccggggc
     2521 gagaccatca tgacggtctc gaacctgaag aagagctacg gaagcctcga agtcctgcgt
     2581 gacatcgacc tcgacatcaa gaagggtgag gtcatctgcg tactcggacc gagcggctcc
     2641 ggcaagagca ccatgttgcg gtgcctgaac ctgctcgagc tgccgaccgg tggcaccatc
     2701 gcgctcaagg gcgagactgt cttcgacggc gggaagccca agaaccttga tgatctccgg
     2761 cgcgaaaccg gcatggtgtt ccagtccttc aatctcttcc cgaacatgtc ggcgaagcag
     2821 aacgtctcga tcgcccaggt cagggtgcgg ggccgcaaga aggatgaagc cgacaagcgt
     2881 tcggttgacc agttgtgcaa ggtcggcctc gacgagaagg tcgacgagta tccggatcgg
     2941 ctctcgggcg gccagcagca gcgggtggcg atcgcccggg ctctggccat ggatccggag
     3001 atcatgctct tcgacgaggt gaccagtgcg ctcgacccgg aactcgtgaa gggcgtgctc
     3061 gacgtgatgc gtgaactggc cgattccggc atgaccatga tctgtgtgac ccatgagatc
     3121 ggcttcgccc gcgaggtcgc cgaccgggtg atcttcatgg atggtggcta catcgtcg
//
LOCUS       DAOTIP010000160         3170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_36126_length_3170_cov_4.928090, whole genome shotgun sequence.
ACCESSION   DAOTIP010000160 DAOTIP010000000
VERSION     DAOTIP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36126_length_3170_cov_4.928090"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="P5138_04020"
     CDS             complement(<1..475)
                     /locus_tag="P5138_04020"
                     /inference="COORDINATES: protein motif:HMM:NF022181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HRV59770.1"
                     /translation="MNRFGQKPGFPGRRAIVAALLIVALLAAVAGCGSSSTEGSGDNG
                     AGSQQGNSSLNSAAKDRAARIAARKARAKARKRAIRARAKARREARKARAAARLAARK
                     ARAANRRIRLRARALARSGGDVNCGDFASQARAQLYLLPGDPFGLDGDGDGVACAD"
     gene            complement(518..2149)
                     /locus_tag="P5138_04025"
     CDS             complement(518..2149)
                     /locus_tag="P5138_04025"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HRV59771.1"
                     /translation="MAEESSLASGLTRRDALRAGAGAAGGLVLTGGLLGRAIDAMGAP
                     AVIGEGPYGSLGSPDANGLRLPAGFSSRVIARSGEDIGPRPYEFHILPDGMGAFRTKD
                     GGFILTSNSEAPYLPGLAEIGAGAIRFDKNFNVVDAYPILKNTWVNCAGGVTPWHTWL
                     SCEEIDTGKVFECDPWGRKPWNGSNWIERPALGVFKHEACCVDPKGKRLYLTEDLGDG
                     CLYRFTPDKYPDLSSGRLEVAIVQESGKVKWAEVPDPQFTGGTPTRKQVDGATTFRRG
                     EGMWFDGGTVYFATTQDDRVYAYHPESTYLEVLYDGVALGDAAPLHDTDNVTASPISG
                     DLYVCEDPGGNALDGLQVCIISTEGEVAAFCEATPEAHKDSELTGPVFDPTGSRLYFS
                     SQRAKFPGESASAGIIYEISGPFRKTRTEPRDSKKPAVKVRTLGKPTLRRLIRQGQPF
                     MVEVADESLPVEVEMELVAPLRRARGRGSRQATIGRKRVRFGKSGRKQVQVKANPNYR
                     KRLREGRFGRRGRLIVVAKDAAGNRTRVVKPVSFR"
     gene            complement(2237..2692)
                     /locus_tag="P5138_04030"
     CDS             complement(2237..2692)
                     /locus_tag="P5138_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV59772.1"
                     /translation="MRVSAKADYAVRAAAELAASPDGPVKGERLADSQDIPLQFLEHI
                     LLDLKHSGIVRARRGAKGGYWLAKPAEEVSIAEVIRAVEGPIAHVQSAPPESITYTGS
                     AKELQNVWIAVRASLREVLESTSLADLVSGELPEHVRELADDKDAWQPR"
     gene            complement(2815..>3170)
                     /locus_tag="P5138_04035"
     CDS             complement(2815..>3170)
                     /locus_tag="P5138_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRV59773.1"
                     /translation="AIVLWAAGIAHFGHGNVDLGLVGNILLGSVPGVIVGAALMNRVN
                     QDSIRIALGVVLILSGFFTVQKGDPVVWPIAAFVAGLGFALILMAPRWYQHFRPAEAI
                     AAEKEAARTAPNQSD"
BASE COUNT          498 a         1074 c         1057 g          541 t
ORIGIN      
        1 gatcggcaca ggcgacgccg tcaccgtcgc cgtcgagccc gaaggggtct cccggcagca
       61 ggtagagctg ggcccgggcc tggctggcga agtccccgca gttgacgtca ccgccgctac
      121 gggccagggc gcgggcccgg agccggatgc gacgattggc tgcccgggcc ttcctggccg
      181 ccagcctcgc ggccgccctc gccttccgtg cctcgcgtct ggccttcgcc cgggccctga
      241 tcgcccgctt gcgcgccttc gccctcgcct tgcgggcggc aatccgtgcg gcccggtcct
      301 tcgcagccga attgagggag ctgttcccct gctgtgatcc ggcgccattg tctccggacc
      361 cttccgtcga ggaagaaccg cagcccgcca cggccgcgag cagggcgacg atgagcaacg
      421 ccgcgacgat ggcgcgcctg ccgggaaacc cgggtttctg gccgaacctg ttcatcgtcc
      481 cggcatttca ccggtcgggt cggactggaa acccggccta acggaagctg accggcttga
      541 ccacccgggt ccggttgccg gccgcatcct tcgcgaccac gatcagacgg cccctgcggc
      601 cgaacctgcc ctcccggagc cgcttccggt agttggggtt cgccttgacc tgaacctgct
      661 tccggcccga cttcccgaag cggaccctct tgcggccaat cgtcgcctgt cttgagccac
      721 ggccgcgggc cctgcgcagc ggcgccacga gttccatttc aacctcgacc gggaggctct
      781 cgtcggccac ctcgaccatg aaaggctgac cctgcctgat cagcctgcgc agggtcggct
      841 tgcccagggt cctgaccttg acggccggct tcttgctgtc cctgggctcg gtgcgggtct
      901 tgcggaaggg gccgcttatc tcgtagatga tcccggcgga ggccgactca ccggggaact
      961 tcgcccgctg ggagctgaag tagagccggc tgccggtcgg gtcgaagacc ggaccggtca
     1021 attccgagtc cttgtgggcc tcgggagtgg cctcgcagaa ggcggccact tccccctcgg
     1081 tcgagatgat gcagacctgc agaccgtcga gggcgttgcc gcccgggtcc tcgcagacgt
     1141 agagatcacc ggagatcggc gacgcggtca cgttgtcggt gtcgtggagg ggcgcagcat
     1201 cacccagagc caccccgtca taaagaactt ccaggtaggt cgattcgggg tggtacgcgt
     1261 agacccggtc gtcctgggtg gtcgcgaaat agacggtccc gccgtcgaac cacattccct
     1321 cgccgcgccg gaaggtcgtc gcgccgtcga cctgcttgcg ggtcggcgtg ccgccggtga
     1381 actgcggatc cggcacctcg gcccacttca ccttgccgct ttcctggacg atcgcgacct
     1441 cgagccggcc ggaggagaga tccgggtact tgtccggcgt gaaccggtag aggcagccgt
     1501 ccccgaggtc ttcggtcaga tagagccgct tgcctttcgg gtcgacgcaa caggcttcgt
     1561 gtttgaacac gcccagggcc gggcgttcga tccagttgct tccgttccag ggcttccggc
     1621 cccacggatc gcattcgaag accttgccgg tgtcgatctc ctcacaggaa agccaggtat
     1681 gccagggagt gacgccgccg gcgcagttga cccaggtgtt cttcaggatg ggataggcgt
     1741 cgaccacgtt gaagttcttg tcgaagcgga tcgctccggc cccgatttcc gccagcccgg
     1801 gcagataggg cgcctcgctg ttcgaggtga ggatgaagcc gccgtccttc gtcctgaagg
     1861 cgcccatacc gtcaggcagg atgtggaatt cgtagggtcg ggggccgatg tcctcacccg
     1921 atcgggcgat cacccgggac gagaatccgg ccggcaggcg gaggccgttg gcgtccgggc
     1981 ttccgagcga tccgtagggc ccttcgccga tcaccgccgg cgctcccatc gcgtcaatcg
     2041 cgcgccccag caaacctccc gtcaggacga gccctccagc cgcgccggct cccgccctca
     2101 gagcgtctcg cctggtcaac ccgcttgcca aagatgattc ctctgccact tgatctccct
     2161 cgctttgttc cgaatccccg ggagagaaga tacggccttg gcaggtggtc gggtcaccag
     2221 gtcgtgaacc ggaagctcag cggggctgcc aggcgtcctt gtcgtcggcg agttcccgca
     2281 cgtgttcggg cagctcaccg gagacgaggt cggcgaggct ggtcgactcg aggacctcgc
     2341 ggaggctggc acggaccgcg atccagacgt tctggagttc cttggcactg cccgtgtagg
     2401 tgatcgattc gggtggcgcc gactggacgt gtgcgatcgg gccctcaacc gcccggatca
     2461 cctcggcgat cgagacttcc tcagcgggtt tggccagcca gtagccgccc ttggccccgc
     2521 gccgggcccg aacgatgccg gaatgcttga ggtcgagcag gatgtgctcg agaaactgaa
     2581 gggggatgtc ctgcgaatcc gcgaggcgct cgcccttgac cggcccgtcc ggggacgcgg
     2641 cgagttcggc agccgcgcga acggcgtaat cggccttggc tgagacgcgc attgatctag
     2701 taaatcagcc cacaccggag cgagccatcc cgctgcccaa gccggttacg gtatttcgcg
     2761 atcaaatcgg tcagcagaat caaaaaatcc ctatgaccga cggagcaggg gtcgtcagtc
     2821 cgactggttc ggcgccgtcc gggccgcctc tttctcggcc gcgatcgctt ccgcgggacg
     2881 gaaatgctgg taccagcggg gggccatgag gatcagggcg aatccgaggc cggccacgaa
     2941 ggccgcgatg ggccagacga cgggatcgcc cttctggacc gtgaagaagc cggaaaggat
     3001 gagcactact ccgagcgcga tccggatcga gtcctggttg acgcggttca tcagcgcagc
     3061 accgacgatc acccccggga ccgatccgag caggatgttg ccgaccagac cgaggtcgac
     3121 gttgccgtgg ccgaagtggg cgataccagc cgcccagagc acgatcgcgg
//
LOCUS       DAOTIP010000161         3155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_36372_length_3155_cov_2.542903, whole genome shotgun sequence.
ACCESSION   DAOTIP010000161 DAOTIP010000000
VERSION     DAOTIP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36372_length_3155_cov_2.542903"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="P5138_04040"
     CDS             complement(<1..295)
                     /locus_tag="P5138_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59774.1"
                     /translation="MILASIFPAQITALRATGLAIAILIIAFALIRRRHLRNADVILL
                     LLAGFGLAIVSGTELTDRLLSALSFNRGNGGRILGLAVIAIFILFLMALRALSV"
     gene            complement(292..1797)
                     /locus_tag="P5138_04045"
     CDS             complement(292..1797)
                     /locus_tag="P5138_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59775.1"
                     /translation="MQVLKSYFTRYGRGATIALAVIVCAGFLVRAWVVVNPLADPGDD
                     ALAYRALAEALYVDGTFGYQAGSSSGPEFKSPSDWSPGAPLIYAGAYYLTGGVRDGVA
                     RGVEALFGAASILLAFLLTARLLGPPGAASGPSGRSRRQGSDGRTEKNLLDESTVSWR
                     QALPPLVAAGLVAFYPPFIHSTGALMSEPPAFFMLPASVLAFLWADRRMNTPGMSGWS
                     RAWPWLLPGLGFGLTALIRPEYLSVCAAFTLFLLIRAWMKSNAANAIASAALFVLAVL
                     IPIVPWTIHNYQELNKVVPLSTGSGKALFTGTYYPGDGEYQQVKAELLYQQTGRRLDP
                     GSAELEAVDPVPLFDRVANDYKKEHDLPDLDRDDALGRLGKENLKKYFGDHPFGYIGM
                     TFRKVGRMWSSGVGDAMSSPVGKVVQILLVLAGLAGLALLAWRRRWEVIAVGLPIATV
                     TAVAAITLAPARRNEILMMLVLPLAAVALDALLNRAPGRAAPDADAATETP"
     gene            complement(1807..2805)
                     /locus_tag="P5138_04050"
     CDS             complement(1807..2805)
                     /locus_tag="P5138_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59776.1"
                     /translation="MSEAPGIRTVGDLRESGHRDKHLREEIRDNLLAMLRARDRLGVA
                     GRPLALRALDWLLPGVLLGMTAMVRPEYLPISIVLILVLAFITRDHGLLPVIGASTVM
                     LLAIVLVIAPWTTRNLDHSGRLVPLSTGGGQTLFTGSYLASAGDPLEVMPDVLARYPG
                     IAARIDAQNRASGEGADSITPERVLALLAARRMPGVPTDLALARIGRENYLRMLRDDP
                     AGLFGYFVRKSARIWWRGRTDLTGTPLGRAVHMTLVAVALMGLLLLGFRRRPEFWLIL
                     ALALAATVIGAVFVASPRRTLAFWPVIACLSGLGFSMALRLARDAVASRLKPVPIA"
BASE COUNT          581 a         1031 c         1113 g          430 t
ORIGIN      
        1 cgaccgagag ggcgcgcaag gccatcagga agaggatgaa gatcgcgatg accgcgaggc
       61 cgagaatccg gccaccgttg ccgcggttga aggaaagcgc cgatagcagc cggtcggtca
      121 actcagtgcc ggaaacgatc gccaggccga agccggcgag caggaggagg atcacgtcgg
      181 cgttccgcag gtgccgtcgc ctgatcaagg cgaaggcgat gatcaggatc gcgatcgcga
      241 ggccggtggc ccggagggcg gtgatctggg cagggaatat cgaggcgagg atcatggggt
      301 ttcggttgca gcgtcagcat ctggcgcggc acggcccgga gcccggttca gcagggcgtc
      361 gagcgcgacc gccgccagcg gcaggacgag catcatcagg atttcatttc ggcgggcggg
      421 agcgagggtg atcgcggcga ctgcagtaac cgtggcaatc ggaagcccga ccgcgatcac
      481 ctcccagcgc cgtcgccagg caagcagggc caggcccgcc agtccggcca ggaccagcag
      541 gatctgaacc accttgccga ccggtgacga catcgcgtcg ccgaccccgg aactccacat
      601 ccggccaacc ttgcggaagg tcatgccgat gtacccgaac gggtgatcgc cgaaatactt
      661 cttcaggttc tccttcccga gccggcccag cgcatcgtca cgatcgaggt ccggaaggtc
      721 gtgttccttc ttgtagtcgt tggcgacccg gtcgaagagc gggaccggat caacggcctc
      781 cagctcggcc gaacccggat cgagtcgccg cccggtctgc tggtagagca gttcggcctt
      841 gacctgctgg tattcgccgt cccccgggta gtaggtgccg gtgaacaggg ccttgccgct
      901 gccggtcgag agcggcacca ccttgttcag ctcctggtag ttgtggattg tccagggaac
      961 tatcgggatc agcaccgcca ggacgaaaag ggcggcggag gcgatcgcgt tggccgcgtt
     1021 tgacttcatc caggcccgga tcaggagaaa gagcgtgaag gcggcgcaga ccgagaggta
     1081 ttcggggcgg atcagcgcgg tcagtccgaa gccgagaccg ggcagcagcc acggccaggc
     1141 ccggctccag ccgctcatcc cgggcgtgtt catgcggcgg tccgcccaga ggaaggcgag
     1201 cacggaggcc ggcagcatga agaaggcagg cggctcgctc atcagggcgc cggtcgaatg
     1261 gatgaacggg ggatagaagg cgacgagtcc agccgcgacc aggggcggca gtgcctgcct
     1321 ccaactcacg gtcgattcat cgagaaggtt cttttcagtc ctgccgtctg acccctgtct
     1381 tctcgaccgc cctgatggcc ctgaagcagc tcccgggggg ccgagcagac gggcggtgag
     1441 caggaaggca agcaggatcg aagcggcgcc gaacagtgcc tcgacaccgc gggctacgcc
     1501 atcgcgcacc ccgccggtca ggtagtaggc gccggcgtag atcagcgggg cgcccggcga
     1561 ccagtcgctc gggctcttga actcgggtcc gctggaggac ccggcctggt acccgaaggt
     1621 tccgtcgacg tagagggcct cggccagggc tcggtaggcg agggcatcgt cgcccgggtc
     1681 ggccaggggg ttgaccacca cccaggcccg gaccaggaaa ccggcgcaga cgatcacggc
     1741 aagggcgatc gtcgccccgc gtccgtaacg ggtgaaataa ctcttaagga cctgcatccg
     1801 ggccggtcag gctatgggaa ccggcttcaa tcggctcgcc acggcatcgc gcgcaagacg
     1861 gagggccatc gagaagccga ggccggacag gcaggcgatc actggccaga aggccagggt
     1921 gcgacgcggc gaggcaacga agaccgcacc gataacggtc gcggccaggg cgagcgcgag
     1981 gatcagccag aactcgggac ggcgacggaa accgagtagc agcagtccca tgagcgccac
     2041 ggcgaccagc gtcatgtgga ccgcccggcc gagcggggtt ccggtcaggt cggtgcggcc
     2101 tcgccaccag atccgggccg atttcctgac gaagtatccg aacagaccgg cgggatcatc
     2161 acgcagcatc cgcaggtaat tctcccggcc gatcctggcc agggcgagat cggtcggtac
     2221 gccgggcatc ctccgcgcgg cgagcagggc caggaccctt tccggcgtga tcgaatccgc
     2281 tccctccccg ctggcgcggt tctgggcatc aattctcgcg gcaattcccg gatagcgggc
     2341 gagtacatcc ggcatcacct cgagcgggtc cccggccgag gccaggtagc tcccggtgaa
     2401 gagggtctgg ccaccgcccg tggagagcgg aaccaggcgg cccgagtgat cgagattgcg
     2461 ggtggtccag ggggcgatga caagcacgat cgccagcagc atcacggtgc ttgcgccgat
     2521 aaccgggagg aggccgtggt ccctcgtgat gaaggccagg acgagaatca ggacgatcga
     2581 gatcggcaga tattcgggcc tgaccatcgc cgtcatgccg agcaggactc cgggaagcag
     2641 ccagtccagg gcccgaagcg ccagcgggcg tcccgccact cccagccggt cccgggcccg
     2701 gagcatcgcc agcaggttgt cgcgaatctc ctcgcgcagg tgcttgtcgc ggtgaccgct
     2761 ctcgcgcagg tcgccgaccg tccgaatccc gggtgcctcg ctcacctcag ccacgctaat
     2821 tggttgccgg ctccaccgcg acaggcgggg tggcgcaacg ggactcggcc gggggcacgg
     2881 gaggagtccc gatcgagcgg gccacaggaa cgccccgatc tcacagcccc cacccatgtc
     2941 cggctgggcg acgcgacagg ggctctgccg accgtaggga taagccgccc gctccgggcg
     3001 ggcggcgaac gttccgatct cgaaggagaa actcatgtcc accagcaagc gcgctgtctc
     3061 gacggcggtc accgtggtgg ccggcctggc gatcctcggc ggcgccgccg tcgccaacgc
     3121 ggcctcgaac accccggccc ccaccccctc cgctg
//
LOCUS       DAOTIP010000162         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_36480_length_3148_cov_2.784352, whole genome shotgun sequence.
ACCESSION   DAOTIP010000162 DAOTIP010000000
VERSION     DAOTIP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36480_length_3148_cov_2.784352"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="P5138_04055"
     CDS             complement(<1..305)
                     /locus_tag="P5138_04055"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRV59777.1"
                     /translation="MNEDRAAEVRVALIQSSWHKDLVDRIATGFIAEAGELGHPESSI
                     EIVNVTGAFGEGYYYYDKEVIGIRDHHHCTLDTLDAYLENKEKYLWEQALPAKYADFA
                     "
     gene            461..862
                     /locus_tag="P5138_04060"
     CDS             461..862
                     /locus_tag="P5138_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HRV59778.1"
                     /translation="MLFGKEHVDRYRATDGEEGHDWQGTHTLLLTTTGRKSGQQRTTP
                     LIYDPVGDAYAIVASKGGADEPPAWYLNLSAEPSVEVQVKADRFTANARTASAEEKAE
                     IWPQMVEQWPAYNDYQAATEREIPIVLLERA"
     gene            complement(909..2195)
                     /locus_tag="P5138_04065"
     CDS             complement(909..2195)
                     /locus_tag="P5138_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59779.1"
                     /translation="MTSSSEKVQRVYLTLMLGNTLAASFIWGINTIFLLDAGLSNLEA
                     FAANAFFTAGMVIFEIPTGVVADTIGRRVSYMAGTLTLMATTLLYVMLWDISGPFWAW
                     ALVSLLLGLGFTFFSGAVEAWLVDALDATGFTGDLERVFGRGQIVTGIGMLTGSVAGG
                     YVAQITSLGVPFLMRAAVLVVMFFFASFAMKDIGFEPRRAESTVGEMREIARNSVKYG
                     WKVPSVKWIMLAAPFTAGVSFYAFYALQPYLLELVGDPKAYGIAGITAALVAGSQIVG
                     GFLAPRIRHLFRLRTSALLLAVGLGALTLTLIGVIGQFWVAVGLIAIWAMLFSASTPI
                     RQSYINGMIPSRQRATILSFDSMLGSSGGVVVQPALGQVADTSGYATSYLYGAMITVA
                     ALPFILLSRRQRPPADAATGTPQTEASVESETPGPA"
     gene            2236..2679
                     /locus_tag="P5138_04070"
     CDS             2236..2679
                     /locus_tag="P5138_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRV59780.1"
                     /translation="MTKVRRLLALFLVLALGAGIAACGSDSDSGDSDPNGIEGVTWQV
                     MNLATQGAATSLPQGIKPPTLEISDGRAQVFAGCNSGSGDAEVGDTTITFGAIAMTKK
                     SCGQIQNQVEFLVNQVLQGKTTYSVNADGNLIIEKGNDSLVYTKG"
     gene            2833..>3148
                     /locus_tag="P5138_04075"
     CDS             2833..>3148
                     /locus_tag="P5138_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV59781.1"
                     /translation="MTVITETGTTTMKNYVGGQWVDAEGAELHDVINPATGEAIARVP
                     FSSAAHLDDAVRSAREALPAWRGVSTIARARMLFTLREKLEARKHEIARLVAPQPLHG
                     ADH"
BASE COUNT          607 a         1015 c         1020 g          506 t
ORIGIN      
        1 gcaaagtctg catacttggc gggcagcgcc tgttcccaga gatacttttc tttgttttcc
       61 agatacgcgt ccagcgtgtc cagcgtgcag tggtgatggt cccgtatgcc gatgacctct
      121 ttgtcgtagt aataataccc ttctccgaag gcccccgtca cgttgactat ttcaatgctg
      181 gactcgggat gacccagctc gccggcctcg gcgatgaagc cggtcgcgat tcgatcaacc
      241 agatccttgt gccacgacga ctggatgagg gctacccgga cctcagctgc tcggtcttcg
      301 ttcatgtccg tcatcctagc cgcgttgccc gctccttccc cgaagtccct ccacccgggc
      361 cggggccgat ccggaggacg ggatcctccg gccacgatcg aatttcccgg gcggcacccg
      421 aatgtccccg ggcgatgccg atcggggcta ggatcgaacc atgttgttcg gaaaagagca
      481 cgtcgatcgt tatcgggcca ccgatgggga agagggacat gactggcagg gcacccacac
      541 cctgctgctc accaccacgg ggcgaaaatc cggccagcag cggaccacgc cgttgatcta
      601 tgacccggtc ggcgatgcct acgcgatcgt cgcctcgaag ggcggtgcgg acgagcctcc
      661 cgcctggtac ctgaacctct cggcagagcc atcggtcgag gtgcaggtca aggccgacag
      721 gttcacggcg aatgcccgca cggcgagtgc cgaggagaag gcggaaatct ggccgcagat
      781 ggtcgagcag tggcccgcct acaacgacta ccaggcggcg accgagcgcg agatccctat
      841 cgtcctgctc gaacgggcct gattcaacgg gtctgaccgc agcgggccgg caggccttcc
      901 ccggccgatc aggccgggcc cggggtttcc gactcgaccg aagcctccgt ctgcggggtg
      961 ccggttgcgg cgtcggcggg tggccgctgg cggcgggaaa gcaggatgaa agggagcgcg
     1021 gcgaccgtga tcatggcccc atacaggtac gaggtcgcat agccggacgt gtcggccacc
     1081 tggcccaggg ccggctggac cacaactccg ccggacgatc cgagcatcga atcgaaggag
     1141 aggatcgtcg ctcgctgccg ggatgggatc attccgttga tgtacgactg gcggatcggg
     1201 gttgacgccg agaagagcat ggcccagatc gcgatcagcc ccacggcgac ccagaactgc
     1261 ccgatcaccc cgatcagggt cagggtcagg gccccgaggc cgaccgccag aaggagggcg
     1321 gaggtgcgga gccggaaaag gtgacggatc cgcggcgcga gaaagccgcc gacgatctgg
     1381 gagcccgcga ccagcgcggc ggtgatcccg gcgattccat acgccttcgg gtctccgacc
     1441 agttccagca ggtagggctg gagcgcgtag aacgcgtaga agctcactcc ggcggtaaac
     1501 ggagcggcaa gcatgatcca cttgaccgac ggcaccttcc acccgtattt cacggagttt
     1561 cgtgcgatct cccgcatctc gccgaccgtg ctctcggccc ggcgaggctc gaagccgatg
     1621 tccttcatcg cgaacgaagc gaagaagaac atgaccacca gcaccgccgc ccgcatcagg
     1681 aacggaactc cgaggctggt gatctgggcc acatagccgc cggcgaccga gccggtcagc
     1741 atcccgatcc cggtcacgat ctgtccccgt ccgaagaccc gctccagatc cccggtgaag
     1801 cccgttgcat cgagggcgtc gaccagccag gcctcgaccg caccggagaa gaaggtgaac
     1861 ccgagaccga gcagcaggga aacgagcgcc caggcccaga acgggccgct tatgtcccag
     1921 agcatcacgt agagcagggt cgtcgccatc agggtcaggg ttccggccat ataggagacc
     1981 cgccgcccga tcgtgtcggc gactacgccg gtcgggatct cgaagatcac catgccggcg
     2041 gtgaagaatg cgttcgcggc gaaggcctcg aggttggaga gccccgcatc gagcaggaag
     2101 atcgtgttga tcccccagat gaaagaggcg gccagggtgt tgcccagcat cagggtcaga
     2161 tagacccgct ggaccttctc cgacgaggag gtcacaggtg gacggtaccc gttgtcggtc
     2221 ggctaacctg cgggcatgac caaggtgagg agacttctgg cgttgtttct ggtgctcgcg
     2281 ctcggggccg gtattgcggc ctgcgggtcg gattccgatt ccggtgattc ggacccgaac
     2341 gggatcgaag gagtgacctg gcaagtgatg aacctcgcga cccagggtgc ggcgacgagc
     2401 ctcccgcagg ggatcaagcc tccgactctc gagatcagcg acggcagggc ccaggtcttt
     2461 gccggctgta acagtggctc cggcgatgcg gaagtcggcg acacgacgat caccttcggg
     2521 gcaatcgcca tgacaaagaa gtcatgcggc cagatccaga accaggttga attcctggtc
     2581 aaccaggtgc ttcagggcaa gacgacctac agcgtgaacg cggacggcaa cctgatcatc
     2641 gaaaagggca acgacagcct cgtttacacc aagggctgat ctccggccgg ccggctagat
     2701 cccggttgcc tcgcgccccc gtctcaccaa gcgtcaaatc tgaggctgag acttcacgga
     2761 tcgtccactt tcgctcgctt tggcgggcgt tagcctgagg tagtccagca tttcccccga
     2821 ggaggctttc gcatgaccgt catcaccgaa accggaacga ccacgatgaa gaactacgtt
     2881 ggcggccagt gggtcgacgc cgaaggggcc gaactccacg acgtgatcaa ccctgccacc
     2941 ggcgaggcca tcgcccgggt ccccttttcc tcggctgccc atcttgacga tgccgttcgc
     3001 agcgcccggg aggcccttcc ggcctggcgc ggggtcagca cgatcgcccg ggccaggatg
     3061 ctcttcactc tccgcgagaa gcttgaggcc cgcaagcacg agatcgcccg gctggttgcg
     3121 ccgcagccgc tgcacggcgc ggaccacg
//
LOCUS       DAOTIP010000163         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_36578_length_3141_cov_3.452041, whole genome shotgun sequence.
ACCESSION   DAOTIP010000163 DAOTIP010000000
VERSION     DAOTIP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36578_length_3141_cov_3.452041"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1109
                     /locus_tag="P5138_04080"
     CDS             153..1109
                     /locus_tag="P5138_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRV59782.1"
                     /translation="MTQVESIGADLGGTKLAVGVVDGMPETLWEEEVPSKGYSQERVV
                     NLLAQQINKAHEARPDASSVGVGIPATLDHESGHAISTVNLDLEDLPIAEMLTEQVKL
                     PVAIDNDGNLAMLAEALYGAARGTRYALLLTIGTGIGGGIWSGGEIYRGATGAGAELG
                     HLVVDIDGHPCQGNCPNRGCVEAMASGTAIGRHGREAAGRAPESLLGKRLAAGDEIDA
                     KVVHEAARDGDDAAISAVNLAGHYLGQALVSYSNIFQPEVIVLGGGAIAFGELLIAPA
                     RREVERYALKPMNRTPVVAAELGPAAGMIGAAAYGRMEAEAA"
     gene            1109..1936
                     /gene="rsmI"
                     /locus_tag="P5138_04085"
     CDS             1109..1936
                     /gene="rsmI"
                     /locus_tag="P5138_04085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRV59783.1"
                     /translation="MPGRLTVCPTPIGNMDDVSKRVREALVSADFVACEDTRRTGRLL
                     ERLEIRPKPRLVSNHEGNEASRAVELVKRMERGDKIALVSDAGMPAISDPGYRLIRAC
                     IERDIDVEVLPGPSVIPVALVASGLPCDRWRFEGFLPKRAGEMERVLNSGETVIAFES
                     PRRISNSLRALAQLAPDRQAAVCREMTKIHEEVSRGTLAELAMRFQGEVKGEIVLVLA
                     PAAAAGHGQDIAFAVEALKRLVKSGARPRAAATVVASLTGTRANDLYKALTGREPRR"
     gene            1933..2847
                     /locus_tag="P5138_04090"
     CDS             1933..2847
                     /locus_tag="P5138_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59784.1"
                     /translation="MSPAAGSKVAYVTCEPRMEQWEDDLLSAGLLTGRGVEVEFVAWD
                     DPVADWESFDLAVVRSTWDYTDRLEEFLEWADSVGPQRLRNVPAMLRWNTDKRYLAEL
                     DGAGLPVPPTALVAPGGTAPPFGGKVVIKPVSGAGARDTGVFDEGNEAEGLALLEKLG
                     AQDEIAMIQPYIPWVDERGETAVLFFGGRFAYALKKRAFLPESGVAPVRPGTTVAEGM
                     FEEDLMSLAEATEAEVELGAKTVAWLARRFGSVPLYARIDMVSSPEGEPVLMEVEATE
                     PSLYLELAAGLEVSGAELFATAVEAALV"
     gene            2870..>3141
                     /locus_tag="P5138_04095"
     CDS             2870..>3141
                     /locus_tag="P5138_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV59785.1"
                     /translation="MSFYVTTPIFYGNGDPHLGHAYSTMAADILARHMRQRGEDVFFL
                     TGTDEHGEPIAQAAEKQGMTPQELVDRSADRFRDMVARVDATNDFF"
BASE COUNT          537 a         1020 c         1094 g          490 t
ORIGIN      
        1 ccgcctgctt ctctttcccg ctcagggagc cgagatcagg cttccggaac cgcgagaaga
       61 ggccccggcg ggagcgatag acgttgtagt cgggacggtc cgggtcgggc ttgttacctg
      121 aggaaggcat gaagagttaa gaatggtggc tgatgacgca ggttgaatcg atcggggctg
      181 atctgggcgg gacaaagctt gccgtcggcg tggtcgatgg gatgcctgaa accctttggg
      241 aagaagaggt cccttcaaaa ggttattcgc aggagagggt cgtgaacctg ctcgcgcaac
      301 agatcaacaa ggcccatgaa gcccgtcccg acgcttcgtc ggtcggtgtc gggatcccgg
      361 ccacgctcga ccacgagtcc gggcacgcga tcagcacggt caatctcgac ctcgaagacc
      421 tgccgatcgc agagatgctc accgagcagg tgaagctgcc cgtcgcgatt gacaacgacg
      481 gcaacctggc gatgctcgcc gaagcgctct acggcgccgc ccgcggaacc cggtacgccc
      541 tgctgctcac catcggaact ggcatcggcg ggggcatctg gtcgggcggg gagatctacc
      601 gaggcgcaac cggcgccggg gcggaactgg gccatctcgt ggtcgacatc gacggccacc
      661 catgccaggg caactgcccg aaccgcggct gcgtcgaggc aatggcatcc ggcacggcaa
      721 tcggccggca cggacgcgaa gcggccgggc gcgcgcctga atcgctgctc ggcaagcggc
      781 tggccgccgg ggacgagatc gacgccaaag tcgtgcacga agccgcgagg gacggcgatg
      841 acgcagcgat cagcgccgta aacctggccg gccactatct cggccaggcc ctggtctcgt
      901 actccaacat cttccagccc gaggtgatcg tgctgggtgg cggggcgatc gctttcggcg
      961 agttgctgat cgccccggcc cgccgggagg tcgaacggta cgcgctgaaa ccgatgaacc
     1021 ggaccccggt cgtcgcggcg gagctcggcc ccgctgccgg gatgatcggc gccgccgcct
     1081 acggccgcat ggaagcggag gccgcctgat gcccggccgt ctgaccgtct gcccgacccc
     1141 gatcggcaac atggatgatg tgagcaagcg ggtccgcgag gcgttggtct cggccgactt
     1201 cgttgcctgc gaggacaccc gccgcacggg ccggctgctc gagcggctgg agatcaggcc
     1261 gaagccgcgg ctggtttcca accacgaagg caacgaggcc tcaagggctg tcgagctggt
     1321 caagcgcatg gaacgcggag acaagatcgc gctggtctcc gatgccggaa tgccggcgat
     1381 ctccgacccc ggatatcgcc tgatccgggc atgcatcgaa cgtgacatcg acgtcgaggt
     1441 gctgcccggg ccctcggtga tcccggtggc cctggttgcc tcggggctgc cctgtgaccg
     1501 ctggcgtttc gagggtttcc tgcccaagcg ggccggtgag atggaacggg tcctgaattc
     1561 gggcgagacc gtgatcgcct tcgagtcacc ccggcggatc tcgaactcgc tgcgggccct
     1621 ggcccagctt gccccggacc gccaggccgc cgtttgccgg gagatgacca agatccacga
     1681 agaagtctcc cgcggcacac tggccgagct ggcgatgcgc ttccagggcg aggtgaaggg
     1741 cgagatcgtt ctcgttctgg cgcctgccgc ggcggccggg cacggtcagg acatcgcctt
     1801 cgcggtcgaa gcgctgaagc gcctggtcaa gtccggcgcc cggccgaggg ccgccgccac
     1861 ggtggtcgcc tcgctgaccg gcaccagggc caatgacctg tacaaggcgt tgaccggccg
     1921 ggaaccccgg cgttgagccc cgccgccgga tcgaaggtcg catatgtcac ctgcgagccc
     1981 cggatggagc agtgggagga cgacctgctc tccgccgggt tgctgaccgg ccggggcgtc
     2041 gaggtcgaat tcgtcgcctg ggatgacccg gtggccgact gggaatcgtt cgacctggca
     2101 gtggtcaggt cgacctggga ttacacggac cggctcgaag aatttctcga atgggccgat
     2161 tcggtcgggc cgcagcggct gaggaacgtg ccggcgatgc tccggtggaa cacagacaag
     2221 cgctacctcg ccgagctcga cggagccggg ctgccggtgc ctccgaccgc cctggtcgct
     2281 ccgggcggga ccgcaccacc cttcggcggc aaggttgtga tcaagcctgt ctcgggagcc
     2341 ggagcccggg acaccggggt cttcgacgaa ggaaacgagg cggaagggct ggctctgctc
     2401 gagaagctgg gcgcacagga cgagatcgcg atgatccagc cctacatccc gtgggtcgac
     2461 gagcgcggcg agacggcggt gctcttcttc gggggccgtt tcgcctacgc cctcaagaag
     2521 agggctttcc tgcccgaatc gggggtggcg ccggtccggc ccggcaccac ggtggcagaa
     2581 ggaatgttcg aagaggacct gatgagcctg gccgaggcga ccgaagccga agtggaactg
     2641 ggagcgaaga ctgtcgcctg gctggctcgc agattcggct ccgtgccgct ctacgcccgg
     2701 atcgacatgg tctcctctcc cgaaggcgag ccggtgctga tggaggtcga ggccaccgag
     2761 ccgagcctct acctggaact cgccgccggc ctggaggttt ccggagccga gctctttgcc
     2821 accgcggtcg aagcggcttt ggtctgaccc gccctatgct ggactgacct tgagcttcta
     2881 cgtcaccaca ccgatcttct acggcaacgg cgacccgcac ctcggccacg cctactcgac
     2941 catggccgcg gacatcctcg cccggcacat gcgccagcgc ggtgaagacg tcttctttct
     3001 caccggaacc gacgagcatg gcgagccgat cgcccaggcg gcggaaaagc agggcatgac
     3061 tccgcaggag ctggtcgacc gcagcgccga ccgtttccgc gacatggtcg ccagggttga
     3121 cgcgaccaat gatttcttca t
//
LOCUS       DAOTIP010000164         3130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_36745_length_3130_cov_2.898211, whole genome shotgun sequence.
ACCESSION   DAOTIP010000164 DAOTIP010000000
VERSION     DAOTIP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36745_length_3130_cov_2.898211"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="P5138_04100"
     CDS             <1..675
                     /locus_tag="P5138_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59786.1"
                     /translation="QGSVTNVARYTSDIEGMTRSSYYQDSANPPSNSSMLCSGDSHSY
                     GAAGPMLTTPQNNTDPTLVGNYPDLPLSHFESVRYSWFDGPEADAGLAELRSEQVDNP
                     LEVETQGIVDPVPETRPARLKLTEKPHLIKARPGQKRRIRLTVRNVGQEPALGVKVCL
                     KAPRRVSHSRCRRPGRIGPSGSRKLILTVRPKRGLRRTRAKVRVKLSSISGGRVTRTV
                     RLLIRR"
     gene            749..2032
                     /locus_tag="P5138_04105"
     CDS             749..2032
                     /locus_tag="P5138_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV59787.1"
                     /translation="MKVAIVGTGIAGLSAAHRLGPLYRAGRLDLKVFEAQGRTGGHAN
                     TVEVESEQGAERIDTGFIVFNERNYPEFTRMLGELGVESKASSMSFSVSDGEFEFTGR
                     SLRGLFARRRHLADPGFIRMLLEVPRLQRRLRELAGSGEEGPSLAEFISGEGFSDELN
                     EKVVVPLVASVWSADPGRMEEFPAGFLARFLDNHGLLSLRDRPRWRTVCGGSRTYVEA
                     LTEPMAGAIRTESRVERIIRRGDGVMVTVAGQPPEQFDRVIVATHSDQALRMIGDPTP
                     EEDHLLRAVPYQANEAVLHTDESLMPVRRSAWACWNYHLGKEARGRSALTYDMNRLQG
                     LGCRRRFFVSLNVTEQIDPGKILARFRYEHPVFTPEGIRAQSRWAEISGSDRIHFAGA
                     WLRNGFHEDGAVTGRRAAEQALLPARAELQLALAA"
     gene            2029..2886
                     /locus_tag="P5138_04110"
     CDS             2029..2886
                     /locus_tag="P5138_04110"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRV59788.1"
                     /translation="MNPGRESAIYEGRIRHRRFEPVEREFDYGIFMLYLDLDELPEVM
                     SIHPLWSTRPRSPAGFRRSDYLGPPDLPLKGAVLDEVERRCGVRPRGPVRMLSHLRYF
                     GVCFNPVTFYYCFDPSGERLETVLLEVTNTPWGERHCYLVDQRRRPRVLAADVAKSLH
                     VSPLMAMDHRYELACGEPDRTLSVHMASRRKGVLHFDATLRMERTEITRARLGRLLST
                     RLPMPLKVLGGIHLEAARTWLAGARYHPHPEKIREEDLSKLPKFGKLCSVKRVSGSGS
                     AGERPLGGG"
     gene            2889..>3130
                     /locus_tag="P5138_04115"
     CDS             2889..>3130
                     /locus_tag="P5138_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59789.1"
                     /translation="MWRRLVLAVIGRLKTGRIEFVENGKVTAIGEPGGTPSAKVTVNS
                     PRAWRRMLRGSTGMAEGYINREWDTDDLVAVGLVAGL"
BASE COUNT          563 a          994 c         1021 g          552 t
ORIGIN      
        1 cagggttcgg tcaccaacgt ggcccgctac acctcggaca tcgagggcat gacccgcagc
       61 tcctactacc aggattcggc gaacccgccc tccaacagtt cgatgctctg ttcaggagac
      121 agccactcct atggagcggc cggtccgatg ctgaccacgc cgcagaacaa caccgacccg
      181 accctggtcg gtaactaccc ggaccttccc ttgagtcatt tcgagtcggt caggtactcc
      241 tggttcgacg gaccggaagc cgacgccggt ctggccgagc ttcgctccga acaggtcgac
      301 aatccgctcg aagtggagac tcagggaatc gtcgaccccg taccggagac caggccggcc
      361 cgtctgaagc tcaccgagaa gccgcatctg atcaaggccc gtcccggcca gaagcggcgg
      421 atcaggctga ccgtgagaaa cgtgggccag gagccggctt tgggggtgaa ggtctgcctc
      481 aaggcacccc gtcgggtaag ccactcccgc tgccgcaggc cgggcaggat aggaccctct
      541 ggcagccgca agctgatcct gacggtcagg cccaaacgag gcctccgtcg cacacgagca
      601 aaggtcaggg tgaaactgag ttcgatttct ggcggccgcg tgaccaggac ggtcaggctg
      661 cttatccgcc gctgaccagg caagagtcgc cagagggggc gccggcacag gactgatccg
      721 gtccgcctcc ggactcgtat ccggaggtat gaaagtcgcg atagtcggga ccgggatcgc
      781 cggtctctcc gccgcccatc gtctcggtcc gctctaccgg gcgggtcggc tggatttgaa
      841 ggttttcgaa gcgcaaggtc ggaccggagg ccatgcgaac acggtcgaag tcgagtcgga
      901 gcagggcgcc gaaaggatcg acaccgggtt catcgtcttc aacgaacgca actacccgga
      961 gttcacccgg atgctgggcg aactcggagt tgaaagcaaa gcctcctcga tgagtttctc
     1021 ggtcagtgac ggcgaattcg aattcaccgg tcgcagtctg cgcgggctct tcgcccggcg
     1081 ccgccatctc gccgatccgg gcttcattcg catgctcctc gaggttcccc gtctgcagcg
     1141 tcgactgagg gaacttgccg ggagtggcga ggaaggtccg tctctggctg agttcatctc
     1201 cggcgaaggt ttctccgacg agttgaacga gaaggtggtc gtgccgctgg tcgcatcggt
     1261 ctggtccgcc gaccccggaa ggatggaaga gttccccgcc gggttcctcg cccgattcct
     1321 tgacaaccac ggtctgcttt cgcttcgcga ccggccccgc tggcgcaccg tttgtggtgg
     1381 ttcgcgaacc tatgtcgaag cgctgaccga gccaatggcg ggggcgatcc ggacggaatc
     1441 cagggttgag cggatcatcc gccgtggcga cggggtgatg gtgacggttg ccgggcagcc
     1501 tcccgagcag tttgaccggg tcattgtcgc gacccactct gaccaggccc ttcgcatgat
     1561 cggcgatccg accccggagg aagaccacct gcttcgggcc gtcccgtatc aggcaaacga
     1621 agcggtgctt cataccgatg agtccttgat gccggtccgg cgttcggcct gggcatgctg
     1681 gaactaccac ctcggtaagg aggcccgggg acgatccgcg ttgacctatg acatgaaccg
     1741 cctgcaaggc ctcggatgtc gccggcgctt cttcgtttca ctcaacgtca cggaacagat
     1801 cgacccggga aagatcctcg cccggtttcg ttacgagcat cccgtgttca caccggaagg
     1861 gattcgggca cagagccgct gggcggagat cagcgggtcg gaccggatcc actttgccgg
     1921 tgcctggctc aggaacgggt ttcacgagga cggggccgtg accgggcgca gggctgccga
     1981 acaggccctg cttccggccc gagcggagct gcaactggcg ctggccgcat gaacccgggc
     2041 agggagagcg ccatatatga agggcggatc aggcaccgcc gcttcgaacc cgtcgagcgt
     2101 gaattcgact acgggatctt catgctctac ctggacctcg acgagttgcc ggaggtcatg
     2161 tcgatccatc cgctctggtc gacccgaccg aggtcacccg ccggtttccg tcgctccgac
     2221 tacctcggcc cgccggacct gcccctgaaa ggggcggtcc tggacgaggt tgaacggcgt
     2281 tgcggagtcc ggccccgggg gcctgtccgg atgctgagcc acctgcggta cttcggagtc
     2341 tgcttcaacc ccgtcacctt ctactactgc ttcgaccctt caggcgagcg actcgagacg
     2401 gtcctgctcg aagtcaccaa taccccgtgg ggagaacgcc actgctacct ggtggatcag
     2461 cggcgacggc cccgcgtgct tgctgcggac gtcgccaaga gccttcatgt gtctccgctg
     2521 atggccatgg atcaccgtta cgaactcgcc tgcggggaac ctgaccggac cctctccgtg
     2581 cacatggcga gtcgtcgcaa aggtgtcctt cactttgacg caaccctgcg aatggagcga
     2641 accgaaatca cgagggcgcg acttggacgc ctgctttcca cgaggctgcc gatgccgctc
     2701 aaggtcctcg gcggcattca tctcgaggcg gcaagaacct ggctggcggg tgcccgctac
     2761 cacccgcatc cggaaaagat tcgggaagag gacttgtcga aactcccgaa attcggcaag
     2821 ctctgttccg tgaaacgggt ttccggatcg ggatcggcgg gcgagcggcc actgggtggt
     2881 ggctgagcat gtggcggcgg ctggttctgg cggtaatcgg ccggctcaag accggccgaa
     2941 tcgagttcgt cgagaacgga aaggtcaccg cgatcgggga gcccggtggc actccgtcag
     3001 cgaaggtcac cgtgaattcg ccccgcgcct ggcgacggat gcttcggggc agcaccggga
     3061 tggcggaggg ttacatcaac cgcgagtggg acaccgacga tctggtcgcg gtcggactgg
     3121 tcgcgggact
//
LOCUS       DAOTIP010000165         3112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37009_length_3112_cov_3.528623, whole genome shotgun sequence.
ACCESSION   DAOTIP010000165 DAOTIP010000000
VERSION     DAOTIP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37009_length_3112_cov_3.528623"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="P5138_04120"
     CDS             <1..921
                     /locus_tag="P5138_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRV59790.1"
                     /translation="LGLLMAPRLPVIGSQVNGAYLGISLGPISFQPAELAKIGIVIFL
                     ASYLREHRELLVVGARRFLGITFPPLKHLGPLLVVWGAAMFMLIFIRDLGSSLMFFAA
                     FLALIYVATGRLSFVIIGMAMFLAGAWVIYHTVPHVTDRVDIWLDPYQDPSGAGYQIL
                     QSMFAMADGGLFGEGFAQAMVVIPGTDAPLLPAAQTDLIYTLITSELGLFGACAIIAT
                     YLLVAARGFKVALLANDGFSKLLATGLTTVFAFQAFVIIGGVTRVIPLTGVTLPLVSY
                     GGSSILANFILLALLLLISDRARREAREGI"
     gene            937..2379
                     /locus_tag="P5138_04125"
     CDS             937..2379
                     /locus_tag="P5138_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV59791.1"
                     /translation="MNARIIKLFAFIGVTFAVLVGFTSYWTVFDANNLKNERVNKRPL
                     FEAQKIKRGRILASDGTVIARSVAKGSGPSLRYVRRYPEGGTYGHPIGYSFVEYGNSE
                     FEAYHNSELIGDENEFSSIFDELRGKDPEGDDVFTSIDPNAQATAFSLLGDQPGAVVA
                     IEPSTGAIKAMVSVPPFDPNSVPTDFSALNQDPGAPMVNRVTQGQYPPGSTFKLVTAT
                     AALDSGAITPDDTINAPGSIQIQGHPLANDFDQDFGDINVTTALTNSVNTYFAQLGEK
                     IGTGTLEEYMDRFGFNQKPQLDLPDGQMSLSGVFDNGTLLTGDDAIDVGRLAIGQERL
                     LATPIQMAEVAAAIANDGDLMRPQIWSEVRDPDGRTVKKMDPEKQSDVMSEQTAEELT
                     SAMKDVVNEGTGTAAALSGVDVAGKTGTAEIPDVERCNGLPNQAWFIGFAPADDPQIA
                     VAATVECTEGQGGTVAAPIAAGVMQTLLGE"
     gene            2443..>3112
                     /locus_tag="P5138_04130"
     CDS             2443..>3112
                     /locus_tag="P5138_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRV59792.1"
                     /translation="MRLSEGMVVDNRYRIDYLIGSGGMADVWLAEDLELPRRVALKVL
                     HDRYARDPEFIARFRREAESAASLQHVNIVPIFDRGQVGDTYYIAMAYLEGRTLRDLI
                     SLGLTPPESVAIVRQVLEAAGFAHRRGIVHRDFKPLNVIVDETGLATVTDFGIARAGI
                     SEITEEGSIMGTVHYLSPEQAQGYEVSPQTDLYSIGVILYECLTGRVPFDGETAVAVT
                     LQQLK"
BASE COUNT          538 a         1074 c          965 g          535 t
ORIGIN      
        1 ctcggcctcc tgatggcgcc gcgcctgccg gtgatcggct cccaggtgaa cggggcctat
       61 ctcgggatca gcctcgggcc gatctccttc cagccggccg aactggccaa gatcgggatt
      121 gtcatcttcc tcgccagcta cctgcgggag caccgggagt tgctggtcgt gggtgcgcga
      181 cgctttcttg gcatcacctt cccgccgctg aagcatctcg gcccgcttct ggtggtctgg
      241 ggcgcggcga tgttcatgct gatcttcatc cgcgacctcg gcagttcgct gatgttcttc
      301 gccgccttcc tcgccctgat ctacgtggcg accggaaggc tctcattcgt gatcatcggc
      361 atggcgatgt tccttgccgg ggcctgggtg atctaccaca cggtgcccca cgtgaccgac
      421 cgggtcgaca tctggctcga cccctaccag gacccatccg gggccgggta ccagatcctg
      481 cagtcgatgt tcgcgatggc cgacggaggg ctcttcggcg agggcttcgc ccaggcgatg
      541 gtggtcatcc cggggaccga tgcaccactc ttgccggctg cgcagaccga cctgatctac
      601 accctgatca cctcggagct cggactgttc ggcgcctgcg cgatcatcgc cacctacctg
      661 ctggtggccg cccgcgggtt caaggtcgcg ctgctggcca acgacggctt ctcaaagctg
      721 ctcgccaccg gcctgaccac ggtcttcgcc ttccaggcct tcgtgatcat cggcggcgtg
      781 accagggtca tccccctgac cggcgtcacc ctgccgctgg tctcctacgg cggttcctcg
      841 atcctcgcca acttcatcct gctggcactg ctgctcctga tctccgaccg ggcccgccgc
      901 gaagcccggg aaggcatctg agaaaggcag cggtccttga acgcccggat catcaagctc
      961 ttcgccttca tcggcgtgac gttcgccgtg ctggtcggct tcacttccta ctggacggtc
     1021 ttcgacgcga acaacctcaa gaacgagcgg gtcaacaagc gaccgctctt cgaggcccag
     1081 aagatcaagc ggggccggat cctcgcctcc gacggcaccg tgatcgcccg ctccgtcgcc
     1141 aagggaagcg gcccgtcgct caggtacgtg aggcggtatc cggagggcgg cacctacggc
     1201 catccgatcg gatacagctt cgtcgagtac ggcaactcgg agttcgaggc gtaccacaac
     1261 tccgaactga tcggagacga aaacgagttc tcgtcgatct tcgacgaact gcgcggcaag
     1321 gacccggaag gcgacgacgt cttcacctcg atcgacccga acgcccaggc gaccgccttc
     1381 tcgctgctcg gggaccagcc gggcgcggtc gtcgcgatcg agccttcgac cggcgctatc
     1441 aaggcgatgg tcagcgtgcc gcccttcgac ccgaactcgg tgccgaccga tttcagcgcc
     1501 ctgaaccagg atccgggcgc gccgatggtc aaccgggtca cccagggcca gtatccgccc
     1561 gggtcgacct tcaagctggt aaccgccacc gctgccctcg attcgggcgc gatcaccccg
     1621 gatgacacga tcaatgcgcc cggttcgatc cagatccagg gccatccgct ggccaacgat
     1681 ttcgaccagg acttcggcga catcaacgtc accacggccc tgaccaattc ggtcaacacc
     1741 tacttcgccc agctcggcga gaagatcggc accggcacgc tcgaggagta catggaccgc
     1801 ttcggcttca accagaaacc gcaactcgac cttcccgacg gccagatgtc gctttcgggt
     1861 gtcttcgaca acggaaccct gctcaccggg gacgacgcga tcgacgtcgg tcgcctggcg
     1921 atcggtcagg agaggctgct cgccacgccg atccagatgg ccgaagtggc ggcggcgatc
     1981 gccaacgacg gcgacctgat gcggccccag atctggtcgg aggttcgcga ccccgacggc
     2041 cgcaccgtca agaagatgga cccggagaaa cagtcggacg tgatgtcgga gcagaccgcc
     2101 gaggagctga ccagtgcgat gaaggacgtt gtcaacgaag gcaccggtac cgccgctgcc
     2161 ctgtccgggg tcgatgtcgc cggcaagacg ggtaccgcgg agatccccga tgtcgaacgc
     2221 tgcaacggcc tgccgaacca ggcctggttc atcggcttcg ccccggcgga cgacccccag
     2281 atcgcggtcg cggcaacggt ggaatgcacc gaagggcagg gaggcacggt cgccgccccg
     2341 atcgccgccg gggtaatgca gaccttgctg ggcgagtaac cgcccattta caggaaaaat
     2401 gcggacaatc gcgaaggttc gtgggatgcg gaagggcttt gaatgagact cagtgaaggc
     2461 atggtggtcg acaaccgcta ccggatcgac tacctgatcg gttccggcgg aatggccgac
     2521 gtctggctgg ccgaggacct cgagctgccc cgccgggtcg ccctcaaggt gctccacgac
     2581 cgctacgccc gcgaccccga gttcatcgcc cggttccgcc gggaagcgga gtccgccgcc
     2641 tcgcttcagc acgtgaacat cgtcccgatc ttcgatcgcg gccaggtcgg cgacacctac
     2701 tacatcgcca tggcctatct cgaaggccgc accctgcgcg acctgatcag cctcggtctg
     2761 accccgccgg aatcggtggc gatcgtgagg caggttctcg aggcggccgg cttcgcccac
     2821 cgccgcggca ttgtgcaccg tgacttcaag ccgctcaatg tgatcgtcga cgagaccggc
     2881 ctcgccacgg tgaccgattt cggcattgcc agggccggca tctcggagat caccgaggaa
     2941 ggctcgatca tgggcacggt ccactatctc tcgcccgagc aggcgcaggg atacgaggtc
     3001 agtccccaga ccgacctcta ctcgatcggg gtcatcctct acgagtgcct gaccggcagg
     3061 gttcccttcg acggcgagac cgcggtggcg gtcacgctgc agcagctgaa gg
//
LOCUS       DAOTIP010000166         3108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37088_length_3108_cov_3.588274, whole genome shotgun sequence.
ACCESSION   DAOTIP010000166 DAOTIP010000000
VERSION     DAOTIP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37088_length_3108_cov_3.588274"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="P5138_04135"
     CDS             <1..1967
                     /locus_tag="P5138_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59793.1"
                     /translation="SAASKSCTATRTVLVDNQASNPPTNLRSDQGTDWQAENGFTLKW
                     GIPAGQVAPVIGAVFVLQDLDSGAQVDAGYFGGQDVESAGPIDVPDVGAYKAIVYLVD
                     GATNLGKSAETILRFDDRPPGNVSPVEPEGWISRDELPIQQEIERAEPGGPSGIGGYA
                     MRVSSGGPAEPCSSGVCLAPEITLSGGADLRTGSIGGLSEGSHWITAAAVSGARKSSL
                     EPGSTVVQVDRTPPSSSITGIPNDWVNRPVTVTVHAVDQLSGMLPEAGDDGEPKTVIA
                     ADNYAPYEAPGAIATFAVATEGVNRIRYWAEDLAGNANDGLPGVDGDTHQNPGQAIVR
                     IDTTPPELRFDPNRDPEDPELVSMVADDTDSGVASASIGIRRAGSGEAFTPLTTTGSG
                     GRYLARVPSDDLPQGSYELNATVRDRAGNQSTGNETSIGAPMILNLPVKQPSSLIASL
                     ARGRQVVRGKYGARQFVDGRLTSGGVALANQTVEVVETFMAGSRRGTVTRQITTDGDG
                     RYRTRLTGGPSRGVEVHFGGNRRLSPVSSNMLRLKVKGRVRFRIKPRKLYNGGVVRMR
                     GSVGFSGALPPARGKLVAIQYFDPSRRKWRPVEVLRTDRRGRFRYGYRFRTIASAQRI
                     VFRASALPEAGWPYLPSTSKPRSVIVYPKD"
     gene            complement(2561..>3108)
                     /locus_tag="P5138_04140"
     CDS             complement(2561..>3108)
                     /locus_tag="P5138_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59794.1"
                     /translation="RTAAQLRAEAEKKAVHAAKLDAENAKSRLEEVQDTAVEYARKGV
                     DVPVGAALNLADRVSEVIEDWSDQASAEKKVKGYRADLTKTVKRAERRGTTTRRKATT
                     QAKKRRNSLEKTVRKQRKSFEKTVKTTNKDVNKQIEARVSEVSKRANQVQADVSKVAE
                     EQGKKAQDLVNKVTEQLQSLV"
BASE COUNT          572 a          913 c         1032 g          591 t
ORIGIN      
        1 agtcggccgc atcaaagtca tgcacggcta cccggacggt cctggtcgac aaccaggcat
       61 ccaacccgcc gaccaacctg cgatccgatc aggggaccga ttggcaagcg gagaacggat
      121 tcactttgaa gtggggcatt ccggcgggtc aggttgcccc ggtaattggt gcggtcttcg
      181 tccttcagga cctggactcc ggtgcgcagg tcgacgccgg ctacttcggc gggcaggatg
      241 tcgagagcgc cgggccgatc gacgttcctg atgtgggggc gtacaaggcg atcgtctatc
      301 tggtcgatgg ggctaccaac ctgggcaagt cggcggagac gatcctccgg tttgacgatc
      361 gcccaccggg aaacgtcagt ccggttgaac ccgagggctg gatatcccgg gacgaactcc
      421 cgatccagca ggaaatcgaa agggctgagc cgggcggtcc ctccggaatt ggtggctatg
      481 cgatgagggt ctccagcgga ggacccgccg agccatgcag ctcaggggtc tgtctcgccc
      541 ccgaaataac tctgtcagga ggggcagacc ttcggaccgg atcgattggc ggcctgagcg
      601 aaggaagcca ctggatcacc gcagcggcgg tttcgggagc acgcaagtct tcgctcgagc
      661 caggcagcac agtcgttcag gtcgaccgca cacctcccag ttcctcgatc accgggatcc
      721 cgaatgactg ggtcaaccgc ccggtgacgg taaccgtcca tgcggttgac caactctccg
      781 gaatgttgcc cgaggcaggc gacgacggcg agccgaaaac agtcattgcc gccgacaatt
      841 atgcgcccta tgaggctccc ggagcgattg cgacattcgc cgtcgcaacc gaaggagtga
      901 accggatcag gtactgggcc gaagatctgg ccggcaacgc aaacgacggc ctgccggggg
      961 ttgacggtga tacccaccag aacccgggac aggccatcgt caggatcgat acgacgcctc
     1021 ccgaactccg atttgatccg aaccgggatc cggaggaccc tgaactggtc agcatggttg
     1081 ccgatgacac ggactccggt gtggcttccg cttcgatcgg aattcgcaga gctgggtccg
     1141 gcgaggcatt cactccgctc acgaccaccg ggagcggagg tcggtacctg gcccgcgtgc
     1201 cttcagatga tctgccccag ggctcatacg aactcaatgc caccgtgcgg gatcgcgcgg
     1261 ggaaccagtc gaccggtaac gaaacctcga tcggtgctcc gatgatcttg aacctgccgg
     1321 tcaagcagcc ttcgagtctg atcgcctcgc ttgcccgggg caggcaggtc gttcgaggga
     1381 aatatggagc gaggcagttt gtcgatggca ggctcacgag tggaggtgtg gccctggcaa
     1441 atcagacggt cgaggtggtc gagacgttca tggcgggatc ccgccgcggc accgtaaccc
     1501 ggcaaatcac cactgacggc gacggcagat atcggacgag gctgaccggc gggccatcta
     1561 gaggagtgga ggttcatttc ggtggcaacc ggagactttc cccggtttcg agcaacatgc
     1621 tcaggctgaa agtgaaagga agggtccgtt tccggatcaa gccgcgaaag ctctacaacg
     1681 gcggcgtggt acgaatgagg ggttcggtcg gattcagcgg ggcgctaccc ccggcaaggg
     1741 gcaagctcgt ggcgattcag tacttcgacc cgtccaggag gaaatggcgc ccggtcgagg
     1801 tgcttcgtac cgatcgtcgt gggaggttcc ggtacggcta ccggttccgc accatcgcgt
     1861 ccgcacagcg catcgtcttc cgggcttccg ccctgccaga ggccggatgg ccatatctgc
     1921 cctcgacatc gaagccaaga tcggtcattg tgtaccccaa ggactgatcc ggcagggaag
     1981 gaggcaggat gaggggaata ccacgaccgt cgccggcgat ggttgtcgca ctgatcgcat
     2041 tgatcgtcgc gatcggtgga actgccgttg ccctaccggg gaagttcacg gtaggcaggg
     2101 atgacttgaa gaagtcaagc gtcggagcga gagctttggg gggaatgtac gtgggacgct
     2161 ccttggtact caagtccgaa gatctcgtcg cgggagatgg gcaattcacc gagtctgtag
     2221 ggagcatctt gtgcccctcg aaagctccta ccgctatcga tcctttcgtt ggggggttga
     2281 gcgaaacggc cttcgaagtc aaccgaagcg ccgtgtcaaa caaatggaag gctccccagg
     2341 ggtatcggtt cacggttaga agcgacgacg gtcccgacgt gggctacacg atgaaggtca
     2401 actgcctctt cgctcgttga gctggtccct taaggaccaa cggccccgaa gaggggccgt
     2461 tggcgaggga ggagaggtac tacatcgact gacgaggtta tgaggtcagc cgatcttctc
     2521 cgggttgccg ctctccgtcg gcagccgggc taagtgtggt ctagaccagc gactgaagct
     2581 gctccgtgac cttgttgacg aggtcctggg ccttcttgcc ctgctcctcg gcgaccttgc
     2641 tgacgtcggc ctggacctgg ttggcgcgct tgctgacttc gctcacgcgg gcttcgatct
     2701 gcttgttgac gtccttgttg gtcgtcttga cggtcttctc gaagctcttc cgctgcttgc
     2761 ggacggtctt ctcgagcgag ttgcggcgct tcttggcctg agtggtggcc ttgcgacggg
     2821 tggtggtgcc acgacgctcg gcacgcttca cggtcttggt gaggtcagcg cggtaaccct
     2881 tgaccttctt ctcggcggaa gcctggtcgc tccagtcctc gatcacctcg gacacgcggt
     2941 cggcgaggtt gagggcggcg cctaccggca cgtcgacacc cttgcgggcg tactcgacag
     3001 cggtgtcctg aacctcttcg aggcggctct tggcgttctc ggcgtcgagc ttggcggcgt
     3061 gaacggcctt cttctcggcc tcggcgcgaa gctgggcggc agtgcgcg
//
LOCUS       DAOTIP010000167         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37252_length_3098_cov_3.607953, whole genome shotgun sequence.
ACCESSION   DAOTIP010000167 DAOTIP010000000
VERSION     DAOTIP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37252_length_3098_cov_3.607953"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="P5138_04145"
     CDS             complement(<1..683)
                     /locus_tag="P5138_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV59795.1"
                     /translation="MAQVPDRDTCLELLGRMCLIRRFEEEAGRQYQRAKAGGFLHLAI
                     GEEATIVGTTSVMREDDYLIGTYRTHGHALARGTPADAIMAELFGRETGTSGGRGGSM
                     HIFDAERRFMGGYGIVGGNLPLAAGLAFASKYRDEDTVTVCMFGDGASNTGNFGETMN
                     LAALWELPVVFLVENNLYGMGTSIERHSAVTNLSHKAEGYGIEGERIDGMDVLEVRDR
                     VSAHLEEVRT"
     gene            863..2380
                     /locus_tag="P5138_04150"
     CDS             863..2380
                     /locus_tag="P5138_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59796.1"
                     /translation="MKKILIIALALTGFLLATSSAQALVVDGGIKVTTYGMGKVTGDG
                     IDCGSDCVETYSWNDEQSAPSVLLSAAGTAEGWVATSYSGCSSLPQNGKCLMNLEAGK
                     VKNVTIYFFDVQPPTVRISGYGNPAGESIHLDIESSDNVAVKKIEVLLDGEVVLTKTS
                     DFSHAEFDTTQVPEGDHVVQVRATDGNRNTGESLSYTITVDHTAPDVHFVDPVDATNG
                     ETASFAFASTEPDYISPRCGIQMQGQEEELTSCEIDEIYEEEVPTEGTWEFVVEVEDL
                     VGNIRRISHPFVVDRTAPEAEFTSGPEDGAEVAPGEVGYAWSAADDLPVTQLCSWDEE
                     DAVSCDGSDAFVLSPGSHSFKVVITDAAGNETTLTRSVTVSQPAPDPDPDPDPDSSDR
                     TAPVVKMVAPKQTLRSARKALRLRVRCNEACSGKVVVKGAGGIRFSGRMVLTGAGVAK
                     LKLKPTAKVRRKLNLISIRNRSHVVPRRVKLIATASLSDAAGNTGRASLRFRLGA"
     gene            2465..>3098
                     /locus_tag="P5138_04155"
     CDS             2465..>3098
                     /locus_tag="P5138_04155"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRV59797.1"
                     /translation="MNPRNLLSTVATLIALVAVGITAGAAQAEPQRELVTGEITVEVH
                     AGGTVTGTGINCGIDCYDSRSWYDDEVPPTNRLTATPGTGWAFGGWQGCTSVNGQSNK
                     CDASYSEFGSEPIIAAFYDVMAPSVYLHYPASDQVVGSSMYAGAETGDNDRVARVEYL
                     IDGQVVATSTTGPFDLNIDTSGVSEGAHQFQARAFDPTGNNGITAVRNFVV"
BASE COUNT          581 a         1047 c          964 g          506 t
ORIGIN      
        1 gtcctgacct cctcgaggtg ggcggagacg cggtcgcgga cctcgagcac gtccatgccg
       61 tcgattcgct ccccttcaat cccgtaaccc tcggccttgt gcgagaggtt ggtgacggcc
      121 gagtggcgtt cgatcgaggt gcccatcccg tagaggttgt tctcgaccag gaacaccacc
      181 ggcagctccc agagggccgc gaggttcatg gtctcgccaa agttgccggt gttcgaggcg
      241 ccgtccccga acatgcagac cgtgaccgtg tcctcgtccc ggtacttcga ggcgaacgca
      301 agaccggccg cgagggggag gttgccgccg acgatgccgt agccgcccat gaagcgccgc
      361 tccgcgtcga agatatgcat cgacccgccg cggccgccgc tggtacccgt ctcccggccg
      421 aaaagctcgg ccatgatcgc gtctgcgggg gtgccccggg cgagggcgtg gccgtgggtg
      481 cggtaggtac cgatcaggta gtcgtcctca cgcatcaccg aagtggtgcc gacgatggtg
      541 gcctcctcgc cgatcgccag gtggaggaac cctcccgcct tggcccgttg gtactggcgg
      601 cccgcttcct cctcgaagcg ccggatcagg cacatccggc cgagcagttc aagacaggta
      661 tcacgatcgg gaacctgggc catgcataga ccctagcgat ttgccggggt cgggtttttg
      721 gcttgcctac ttgaatttgt tgttccagcc gacggacctt gcgaaccccg caaagtttcc
      781 gcaagaagac cgaaagctgc tcccggaaag ctgccgccac attcgattcg cccttccccg
      841 aactttcccg aggagtcccg aaatgaaaaa gatcctgatc atcgccctgg ccctcaccgg
      901 cttcctgctg gcaaccagca gcgcccaggc gcttgtcgtc gacggtggca tcaaggtcac
      961 cacctacggc atgggcaagg tcaccggcga cggcatcgac tgcggaagcg attgtgtcga
     1021 gacctacagc tggaatgacg agcagagcgc tccatcagtc cttcttagcg cggccggcac
     1081 ggctgaaggc tgggtcgcga cgagctactc cggatgctcc tcgctgcccc agaacggcaa
     1141 gtgcctgatg aacctggagg cgggcaaggt caagaacgtc accatctact tctttgacgt
     1201 ccagcccccg acggtcagga tctccggtta cggcaacccg gccggtgaga gcatccacct
     1261 ggatatcgag tcttccgaca atgtcgccgt aaagaagatc gaggtcctgc tcgacgggga
     1321 ggtggtgctc accaagacca gcgacttctc tcacgcggaa ttcgacacca cccaggtgcc
     1381 cgaaggagac cacgtcgttc aggttcgcgc cactgatggc aaccgcaaca cgggcgagag
     1441 cctcagttac acgatcacgg tcgaccacac cgcccctgat gtgcatttcg tcgatccggt
     1501 cgacgcgacc aacggggaga cagcctcctt cgccttcgct tcgaccgagc cggactacat
     1561 ctcgccgcga tgcggcatcc agatgcaggg tcaggaagaa gagctgactt cctgtgaaat
     1621 cgacgagatc tacgaggaag aggtcccgac cgaaggaacg tgggagttcg tcgtggaggt
     1681 ggaagacctc gtcggcaaca ttcgccggat cagccatcct ttcgtcgtcg atcgcaccgc
     1741 ccccgaggcg gagttcacct ccggccccga agacggtgcc gaggtcgccc cgggagaggt
     1801 cggctacgcc tggagcgcag ccgacgatct gccggtcacc cagctctgct cctgggacga
     1861 ggaggatgcg gtcagctgcg acggcagcga cgccttcgtg ctctccccgg gcagccacag
     1921 cttcaaggtc gtgatcactg acgcggccgg caacgaaacc accctgaccc ggtcggtcac
     1981 ggtcagccag ccggcaccgg acccggatcc cgacccggat ccggacagtt ccgatcgcac
     2041 cgccccggtg gtgaagatgg tcgccccgaa gcagaccctg aggtcggctc gtaaggcgct
     2101 gcgcctgagg gtccgctgca acgaggcctg ctccggcaag gtggtcgtca agggtgcggg
     2161 cgggatccgg ttcagcggcc ggatggtcct gaccggcgcc ggggtggcga agctgaagct
     2221 gaagccgacc gcgaaggtcc gccgcaagct gaatctgatc tcgattcgca accgcagtca
     2281 cgtcgtgcca cggcgagtga agctgatcgc caccgcaagt ctgagtgacg ccgccggaaa
     2341 caccggcagg gcctcgctcc gattcaggct cggtgcctga gcaaggcacc acctccgtct
     2401 cctccaacag gtgccgtccc gctccccctc cagcggggcg gcgccactcc ctgaaaggaa
     2461 aaccatgaac ccgagaaacc tcctgtcaac cgttgcgacc ctcatcgccc tggttgccgt
     2521 cggcatcacc gccggggcgg cccaggccga gccccagcgt gaactcgtca ccggcgagat
     2581 caccgtcgag gtccacgctg gcggcaccgt caccggaacc ggcatcaact gcggcatcga
     2641 ctgctacgac tccaggtcct ggtacgacga tgaggtgccg ccgacgaacc ggctcactgc
     2701 cacccccgga acgggctggg cattcggcgg ctggcagggc tgcacttcgg tcaacggtca
     2761 gtcgaacaag tgcgacgcca gttactccga attcggcagc gagccgatca tcgccgcctt
     2821 ctatgacgtg atggccccga gcgtctatct ccattacccg gccagcgacc aggttgtcgg
     2881 cagcagcatg tatgccggcg cggaaaccgg tgacaacgac cgggtggcca gggtcgaata
     2941 cctgatcgac ggccaggtgg ttgcgacttc gaccacgggc cccttcgacc tgaacatcga
     3001 caccagtgga gtcagcgaag gtgcacacca gttccaggcc cgggccttcg acccgaccgg
     3061 caacaacggc atcaccgccg tccgcaactt cgtcgtgg
//
LOCUS       DAOTIP010000168         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37697_length_3070_cov_3.801327, whole genome shotgun sequence.
ACCESSION   DAOTIP010000168 DAOTIP010000000
VERSION     DAOTIP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37697_length_3070_cov_3.801327"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="P5138_04160"
     CDS             <1..869
                     /locus_tag="P5138_04160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRV59798.1"
                     /translation="FGSSLRRVLLSSLGGAAITSVRIEGVSHEFSNIEGVKEDVTDIV
                     LNLKDLVVRMHTDADAVEAPIVATGPGNVTAADIDLPAGVEILNPDAPVATLEKKTRL
                     EMYVTIGRGRGYRPAEENKDSDQPIGVIPIDSIFSPIRRASYTVEAARVGQRTDFDKL
                     TLELETDGSIEPNTALREASEILIKSLAIFSDPDRVEELTAREPVVVEGIVADNGAPV
                     ATGGENDILIDELELSVRSYNCLKRAGIETIGQLLAKSESELAAIPNFGQKSIEEVIE
                     NLQGRGHQLRQD"
     gene            900..1253
                     /gene="rplQ"
                     /locus_tag="P5138_04165"
     CDS             900..1253
                     /gene="rplQ"
                     /locus_tag="P5138_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRV59799.1"
                     /translation="MRHAKKRNKLGRDAAHRKALLSNLSKEIIEHERIKTSQAKAKAA
                     KPEIEKLITLGKRGDLHARRQALSVLRQDKFAVHKLFEEIAPRYAERDGGYTRIVKIG
                     PRRSDSTEMVFLELV"
     gene            1510..2916
                     /locus_tag="P5138_04170"
     CDS             1510..2916
                     /locus_tag="P5138_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59800.1"
                     /translation="MAIAATLLLLAAANPARATWGTPILGNAPTGVATDSSGNLYVAE
                     SYGNRFVKYSSTGTQLMAVGSSGNGQAQFNSPNGIAVESGGAIFVADTGNNRIQRFYP
                     NGSYQTEWGGFGTGLGQFKQPSAVAVDGGGNVYIADTDNNRVVKCNAWGWCGSAFDYG
                     SIGGIAGPLGVATDSNGNVYVSDSGNDRIVKSDSSGNVISVWSTYGSGQGQVNDPAGL
                     AIGASNNLFVADKGNSRVQRLSTGGAFLSEWIVPSPYVTSQPTSVAIGPGTTINVANP
                     WNNRVERWDPPTGPTGPTGPTGPTQATGPTGPTSPTGPTGPTGSTTGPVINPDGNNNG
                     NNNSNNNSGNNNSSGNTKKNKKSKVKQAVIKPVKVWMEGGGSVVRPGSAVNMKVSIKN
                     SGDLTARNVYTYMGKKALAGRPSVVVKRGFNTRIITPGWTVTGTVTIFFGKRAHGKVA
                     ITAQAWGNWGKKILYIRR"
BASE COUNT          614 a         1032 c          943 g          481 t
ORIGIN      
        1 ccttcggttc gagcctgcgt cgcgtgctgc tctcttcgct gggcggtgcc gcgatcacca
       61 gcgtccggat cgagggcgtc tcgcatgagt tttcgaacat cgagggcgtg aaggaagacg
      121 tcaccgacat cgtcctgaac ctcaaggacc tggtcgtgcg gatgcacacc gacgcggacg
      181 ccgtcgaggc tccgatcgtc gcgaccggcc ccggcaacgt caccgctgcc gacatcgacc
      241 tcccggccgg cgtcgagatc ctcaacccgg acgctccggt cgccacgctc gagaagaaga
      301 cccgtctcga gatgtacgtg accatcggcc gtggccgcgg gtaccgtccg gccgaggaga
      361 acaaggattc cgaccagccg atcggcgtga tcccgatcga ctcgatcttc tcgccgatcc
      421 gccgtgcctc ctacaccgta gaggcagccc gtgtcggtca gcgcaccgac ttcgacaagc
      481 tcaccctcga gctcgagacc gacggctcga tcgagccgaa taccgctctc cgggaagctt
      541 ccgagatcct gatcaagagc ctcgcgatct tcagcgaccc ggaccgggtc gaggaattga
      601 ccgcccgcga gccggtcgtc gtcgagggca tcgtcgccga caacggcgct cccgtcgcca
      661 ccggtggcga gaacgacatc ctgatcgacg agctcgagct cagcgtccgt tcctacaact
      721 gcctcaagcg tgccggcatc gagacgatcg gccagctgct ggccaagtcc gagtccgagc
      781 tcgctgcgat cccgaacttc ggccagaagt cgatcgaaga ggtcatcgag aaccttcagg
      841 gccgcggcca tcagctgcgc caggactagt cttaccgagt aacccaagga gagaaagaga
      901 tgcgccacgc aaagaagagg aacaagctgg gccgggacgc ggctcaccgg aaggcgctgc
      961 tcagcaatct ctccaaggag atcatcgagc acgagcggat caagaccagc caggccaagg
     1021 ccaaggctgc caagccggag atcgagaagc tgatcaccct cggcaagcgc ggtgaccttc
     1081 acgcccggcg ccaggctctt tcggtcctgc gccaggacaa gttcgccgtc cacaagctgt
     1141 tcgaggagat cgctccccgc tacgccgagc gtgacggcgg gtacacccgg atcgtcaaga
     1201 tcggcccccg ccggtccgat tcgaccgaga tggtcttcct ggagctcgtc tgatcttcgt
     1261 tcagaccggg ttctgagcaa gaaagttgcc gcaaagggga gccaatggct ccccttttgc
     1321 ctgctccgac ctcgccggaa tgctttcccg gctcaagtcg gggcggaagc gctccttttt
     1381 cggtcatttg gtgtttctga ggctgggcct atactgggct attactgaat tccgaattga
     1441 tcgggccagg cattgatttc cttcaggggg cagcaccaaa tccgggcgag aatcatcgcc
     1501 acagcgctag tggcaatcgc cgccactctc ctgctgcttg ctgccgcgaa tccggcccgg
     1561 gcaacctggg gcacaccgat cctgggcaat gccccgaccg gtgtcgccac tgacagctcc
     1621 ggcaacctct atgtcgccga gtcctacggg aaccgcttcg tcaagtacag ctcgaccggc
     1681 acccaactga tggcggtcgg ctcttcgggt aacggtcagg cccagttcaa ttccccgaac
     1741 gggatcgcgg tcgagtccgg tggagcgatc ttcgtcgccg acaccggcaa caacaggatc
     1801 cagcgcttct acccgaacgg cagctaccag accgaatggg gcggtttcgg aaccgggctc
     1861 ggtcagttca aacagccttc ggccgtggcg gtcgatggcg gcggcaacgt gtacatcgcc
     1921 gataccgaca acaaccgggt ggtcaagtgc aacgcctggg gttggtgcgg ctcggccttc
     1981 gattacggca gcatcggcgg tatcgccggc ccgctcggag tggccaccga ctcgaacggc
     2041 aatgtctacg tgtccgactc cggcaacgac cggatcgtga agtccgattc gagcggcaac
     2101 gtgatttccg tctggagcac ctacggttca ggccagggcc aggtcaatga cccggccggc
     2161 ctcgcgatcg gggccagcaa caacctcttc gtcgccgaca agggcaactc gcgggtgcag
     2221 agactctcga ccggtggtgc cttcctcagt gaatggatcg tgccttcgcc gtatgtcacc
     2281 tcgcagccga cctcggttgc gatcggcccg ggcacgacga tcaacgtcgc caatccctgg
     2341 aacaaccggg tcgagcgctg ggacccgccg accggcccga caggcccgac cggacccacc
     2401 ggcccgaccc aggcgaccgg cccgaccgga cccacgtcgc cgaccggacc caccggcccg
     2461 accggatcga ctaccgggcc cgtgatcaac ccggacggga acaacaacgg caacaacaac
     2521 agcaacaaca acagcggcaa taacaacagc agcggcaaca cgaagaagaa caagaagagc
     2581 aaggtcaagc aggcggtcat caagccggtc aaggtctgga tggaaggcgg cggcagcgtc
     2641 gtccggcccg gcagcgcggt caacatgaag gtttcgatca agaacagcgg cgacctcacc
     2701 gctcgcaacg tctacacata catgggcaag aaggccctcg ccggccgtcc gagcgtcgtg
     2761 gtcaagcgcg gtttcaacac caggatcatc accccgggat ggacggtcac cggaactgtc
     2821 acgatcttct tcggcaagcg agcccatggc aaggttgcga tcaccgccca ggcctggggc
     2881 aactggggca agaagattct ctacatccgc cgctgaactg gcgactcgac atcgagtacg
     2941 acggctcggt cttccgcggc tgggcccggc aggacggcgg ggtggacacg gtgcaggccc
     3001 ggctcgagcg tgcgctccag atcgccctgc ggaccgaccg gtcgatcgag ctgacggtcg
     3061 ccggccgaac
//
LOCUS       DAOTIP010000169         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37701_length_3070_cov_3.414594, whole genome shotgun sequence.
ACCESSION   DAOTIP010000169 DAOTIP010000000
VERSION     DAOTIP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37701_length_3070_cov_3.414594"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="P5138_04175"
     CDS             <1..235
                     /locus_tag="P5138_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HRV59801.1"
                     /translation="AMRRDLEAIADEAEREARVRELTAMAEQNARAMNAAQLFEIDDV
                     IDPAETRSVIAATLAAAPQRIADRPPHRFVDTW"
     gene            388..2265
                     /locus_tag="P5138_04180"
     CDS             388..2265
                     /locus_tag="P5138_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59802.1"
                     /translation="MALATVVLLAAVTALMGLASGARAEIYSTESLPSGVQRTTYRLG
                     PVDVTPGQNRIVFRPITGASRPGVNGWITRIKPDLVYGDGSIPKSSKVMFHHGVWINA
                     STGENFYATGEEKTILDLPDGFGYRYKTNEGWMLNDMIHNLTPEAMQLYFEYTIDFIP
                     DSAPEAANIVRARPIWMDVQKGIYPVFDVWRGSGGADGKFTYPQDDPNAYPPGQQLNV
                     KTIPADGVLIGTTGHVHTGGLSTNLYLRRDGASYQGPSCPERISYADQLKPLRKKKVS
                     LAAKIKKIKKRNAKMTRTQKKIRKSAARRGHSAGSKSQIGKLKRKKKANAKMIAKVKR
                     LKARNQKKLNKVEAKDKAERARDAACRATQPQVDDGNRVHLFNSKAKYFDPRGPISWD
                     MAMYSTDNDWRVRVKAGDKLDLQTTYETKRASWPESMGINIVYWARDSAIQAVPGNSD
                     PASAPDPYVTRVDTEGVLNHPHLKENEDYGGESGKNGSKLVGPDPTKLPDGSETGGPF
                     TISDFVYNGADFRLPGALGRPPVVKQGQSFTFKLSDSDITNEVWHSITSCATPCNKST
                     GISYPIPNGKYQFESGQMGPGQEPTVGYTEWSTPANLPKGTYTFFCRIHPLMRGAFRV
                     E"
     gene            complement(2308..2808)
                     /locus_tag="P5138_04185"
     CDS             complement(2308..2808)
                     /locus_tag="P5138_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59803.1"
                     /translation="MPVSVAEIGEWSDYFVATAGASAALAGLVFVAISINVERIIGLK
                     GVAELGLVTLLLLIGVLIVSMFGLIPGQSEHALGAELLVQSLIWSAAIAHFVNRSLAG
                     VGEGEGRLASRLVLPLLGTVPYVVGSILLLAGSDMAMYWVFAGMVGAIIAAVLNAWVL
                     LVEILR"
     gene            complement(2813..>3070)
                     /locus_tag="P5138_04190"
     CDS             complement(2813..>3070)
                     /locus_tag="P5138_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV59804.1"
                     /translation="WVFSEGAAKVVARELIAEARGEEPPAPYDGKGSCYIEFGEDRIA
                     RVDIDFLSGPERTGVFNAPSLELRDQKNQFGSSRESRWFGL"
BASE COUNT          645 a         1041 c          945 g          439 t
ORIGIN      
        1 cgcgatgcgg agggacctcg aggcgatcgc cgacgaagcc gagcgggaag cacgcgttcg
       61 cgaactgacc gcgatggcgg agcagaacgc ccgggcaatg aacgccgccc agctcttcga
      121 gatcgacgac gtgatcgatc cggccgagac ccgatcagtc atcgccgcca ccctggcggc
      181 ggcgccgcag cggatcgccg accggcctcc gcatcgcttc gtcgacacct ggtgagtccc
      241 ggcccgcacc ggccaatctc tctcccggaa cgcaacccga ctgcccgctg ccggttattg
      301 gggtaacgtg aaggtctcgt tgtccgaacg aaccgggaga ggttgatggg aatcggtagg
      361 accgaaacat caaacgcggg cggtcgcatg gccctcgcaa ccgtagtgct gcttgccgcc
      421 gttacggccc tgatggggct cgcgagcggt gcccgcgccg agatctattc aacggaatcc
      481 ctgcccagcg gagtccagcg caccacttac cgccttggcc cggtggacgt gacccccgga
      541 cagaaccgca tcgtcttccg gccgattacg ggtgcctcac gccccggcgt caacggctgg
      601 atcaccagga tcaagccgga cctcgtctac ggagacggat cgattcccaa gtcgagcaag
      661 gtgatgttcc accacggagt ctggatcaac gcctcgaccg gcgagaactt ctacgcgacc
      721 ggcgaagaaa agaccatcct cgacctcccg gacggcttcg gctaccggta caagaccaac
      781 gaggggtgga tgctcaacga catgatccac aacctcacgc cggaagcgat gcagctctac
      841 ttcgagtaca cgatcgactt cattccggac tcggccccgg aagcggcgaa catcgtcagg
      901 gcacgtccga tctggatgga cgtccagaag ggcatctacc cggtcttcga cgtctggcgc
      961 ggcagcggcg gcgccgatgg caagttcacc tatccgcagg acgatccgaa cgcttaccct
     1021 cccggacagc agctgaacgt caagacgatt cccgccgacg gcgttctgat cgggacgacc
     1081 ggtcacgtac acacgggcgg cctctcgacc aacctctacc tccgccggga tggcgcctcc
     1141 taccagggcc cgagttgccc cgagaggatc tcctacgccg accagctcaa gccgctgcgc
     1201 aagaagaagg tgagcctcgc cgcgaagatc aagaagatca agaagcgcaa cgcgaagatg
     1261 acccggaccc agaagaagat ccggaagtcg gccgccaggc gcggacacag cgccggcagc
     1321 aagagccaga tcggcaagct caagcgcaag aagaaggcga acgcgaagat gatcgccaag
     1381 gtcaagcgcc tcaaggcccg gaaccagaag aagctgaaca aggtcgaggc gaaggacaag
     1441 gccgagcgcg cccgcgacgc ggcctgccgg gccacccagc cgcaggtcga tgacggcaac
     1501 cgggtccacc tcttcaactc gaaggcgaag tacttcgacc cccggggacc gatttcctgg
     1561 gacatggcga tgtacagcac cgacaatgac tggcgggtac gggtcaaggc cggagacaag
     1621 ctggatctcc agaccaccta cgagaccaag cgcgcttcct ggcccgagtc gatgggaatc
     1681 aacatcgtct actgggctcg cgactccgct atccaggcgg tccccggcaa ttcggatccc
     1741 gccagcgctc ccgacccgta tgtgaccagg gtcgacaccg aaggggtcct gaaccatccg
     1801 cacctcaagg agaacgagga ttacggcggc gaatcgggca agaacggcag caagctggtc
     1861 ggccccgatc cgaccaagtt gccggacggc agcgagaccg gtggcccgtt cacgatcagc
     1921 gacttcgtct acaacggtgc cgacttccgc ctgccaggtg cgctgggccg tccgccggtg
     1981 gtcaagcagg gccagtcgtt cacgttcaag ctctccgact cggacatcac gaacgaggtc
     2041 tggcactcga tcacttcctg cgcgacgccc tgcaacaagt cgaccggcat ttcctacccg
     2101 atcccgaacg gcaagtacca gttcgaatcg gggcagatgg ggccgggaca ggaaccgacc
     2161 gtcggctaca cggaatggtc gaccccggcc aacctgccga agggcaccta caccttcttc
     2221 tgccggatcc acccgctgat gcggggcgcc ttcagggttg agtgattacc gcatgagtga
     2281 tcgcctccgg aaagccgggg gcgatcctca gcggaggatc tcgaccagca gcacccaggc
     2341 gttgaggacg gcggcgatga tcgccccgac catgccggcg aagacccagt acatcgccat
     2401 gtcactgccg gcaaggagca ggatcgaacc gaccacatag ggcacggttc cgagcagcgg
     2461 caggaccagt ctcgaggcca gcctcccttc tccctcgccc accccggcga gactgcggtt
     2521 gacgaagtgg gcgatcgccg ccgaccagat cagcgactgc accaacagtt cggcgcccag
     2581 ggcgtgttcg gactgcccgg ggatcagtcc gaacatcgag acgatcagca ccccgatcaa
     2641 caggagcagg gtgaccaggc ccagctcggc cacccccttc agcccgatga tccgttcgac
     2701 gttgatcgag atcgcgacga agaccagacc ggccagggcg gcagatgccc ccgcggtcgc
     2761 cacgaagtag tcgctccatt caccgatctc cgctacagaa accggcatcg gctcagagtc
     2821 cgaaccagcg ggactcgcga ctggacccga actggttctt ctggtcacgg agttccagcg
     2881 acggggcgtt gaagactccg gtcctctcgg gaccggagag gaagtcgatg tcgaccctgg
     2941 cgatcctgtc ctcgccgaac tcgatgtagc aggagccctt gccgtcatag ggggccgggg
     3001 gctcctctcc cctcgcttcg gcgatcagct cgcgggcgac caccttggcg gcgccttcgg
     3061 agaagaccca
//
LOCUS       DAOTIP010000170         3066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37771_length_3066_cov_2.586184, whole genome shotgun sequence.
ACCESSION   DAOTIP010000170 DAOTIP010000000
VERSION     DAOTIP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37771_length_3066_cov_2.586184"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="mdlC"
                     /locus_tag="P5138_04195"
     CDS             complement(<1..1334)
                     /gene="mdlC"
                     /locus_tag="P5138_04195"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HRV59805.1"
                     /translation="MPTVREATFDLFRKLGMTTIFGNPGSTELPMLADFPSDFEYVLG
                     LQEAAVVGMADGHAQVTGKPTLVNLHTAPGLGNAVGALFNAQANHSPVVVTVGQQVRA
                     QVTMQANLTNRDAIRVPDPFVKYSYEPARAEDVPLALAHAIHLASLPPMGPVMVSIPM
                     DDWDVEMDATEAQHVIDRAVVGRSGADPAAVKDLAARLKEAKNPVLVAGPDIDASGAW
                     DDAIALAESQNLPVWASPATGGGRLGFPESHPHWRGVLPPGIMAIGEALKDHDLILVV
                     GSSVFPYYPYMPGPFLGENSKLVQITSDPDEAARAPMGEAIVADVKLTLAALNGELDG
                     TDRPAVEPWPGPTRMEETDPLNGSVVMNTLGDVMPEDTVIVLEAPTSTMALRNQLRIS
                     KPNSYFFGAGGGLGFGLSAAVGIQYAEDRPVVCVLGEGSVQYAVTAFWTAAA"
     gene            1418..1945
                     /locus_tag="P5138_04200"
     CDS             1418..1945
                     /locus_tag="P5138_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59806.1"
                     /translation="MRKTLAFALILPALALPFAGCSDGRKIATPPACLSDSSTWVTAL
                     AAAPDQVRISEATPISECLVKDQVAAQQQEVGKTAVEAATRLAAFYKGAGKTGKQTDT
                     GTKVSGGSASSEQAALMAGYLVGALQKGAGETDGIHATLVNRVEAAASNGLDGADQQV
                     QGAYQKGHQAGLDSG"
     gene            2017..>3066
                     /locus_tag="P5138_04205"
     CDS             2017..>3066
                     /locus_tag="P5138_04205"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRV59807.1"
                     /translation="MSEQLWGGETTKAIENFPVSGETIPPSLVKWLGLIKANAARANA
                     ELGLLDADIADRIARAGDAVGSGEHDDQFPIDIFQTGSGTSSNMNTNEVIATLAGEGV
                     HPNDHVNMGQSSNDVFPSAVHLAALDQVTHDLLPAMEELAVALETKAEEFKDVVKAGR
                     THLMDAVPVTLGQEFGGYASQVRHGIDRVRGTLDRVGQIPLGGTATGTGLNTHPEFSA
                     RVRALLEAETGLAISPPHDRFEAQANRDSLVELSGALKVYAVSLNKIANDLALMGSGP
                     RTGLAELHLPELQKGSSIMPGKVNPVIPEVVIQVAAQVVGNDMAITMAGSQGQFELNV
                     RVPLIARNLLGSIRIL"
BASE COUNT          534 a         1032 c         1016 g          484 t
ORIGIN      
        1 tatgcggccg cggtccagaa agcggtgacc gcgtactgga ccgagccttc gccaagcacg
       61 cagacgaccg gccggtcttc ggcgtactgg atcccgaccg cggccgaaag gccgaagccg
      121 aggccgccgc cggctccgaa gaagtagctg ttcggcttcg agatccggag ctggttgcgc
      181 agggccatgg tcgaggtcgg cgcctcgagc acgatcaccg tgtcctccgg catcacgtcc
      241 ccgagggtat tcatcactac cgagccgttg agcgggtcgg tctcttccat ccgggtcggc
      301 ccgggccagg gttcaaccgc cgggcggtcg gtgccatcca gctcgccgtt cagggcggcc
      361 agggtcagct tcacgtcggc gacgatcgcc tcgcccatcg gtgcgcgggc ggcttcgtcc
      421 gggtcggagg tgatctggac gagcttcgag ttctcgccga ggaacgggcc gggcatgtac
      481 gggtaatagg ggaagacgga agagccgacc accaggatca ggtcatggtc cttcagcgct
      541 tcgccgatcg ccatgatccc gggcggcaat acgccgcgcc agtgcgggtg gctttccggg
      601 aacccgagcc ggccgccgcc ggtggcgggc gaagcccaga ccggcaggtt ctgactttct
      661 gccagggcaa tcgcgtcatc ccatgccccg gaagcgtcga tgtccggacc ggcaaccagc
      721 accgggttct tcgcttcctt caggcgggcg gcgagatcct tcaccgcggc cggatcggcc
      781 ccggagcgac cgaccacggc gcggtcgatc acatgctgtg cctcggtggc atccatttcg
      841 acgtcccagt cgtccatcgg gatcgagacc atcaccgggc ccatcggcgg cagcgaggcg
      901 agatggatcg cgtgagcgag ggcgagcggc acatcctcgg cccgggccgg ttcgtacgag
      961 tacttgacga acgggtccgg cacccggatc gcgtcgcgat tggtcaggtt ggcctgcatc
     1021 gtcacctggg cgcggacctg ctgcccgacc gtcaccacga ccggggagtg gtttgcctgg
     1081 gcgttgaaga gggcgccgac cgcgttgccg agcccgggtg cggtgtgaag attgaccagg
     1141 gtcggcttgc ccgtcacctg ggcgtgaccg tccgccattc cgaccaccgc tgcctcctgc
     1201 agtccgagca cgtactcgaa gtcggagggg aaatcggcca gcatcggcag ctcggtcgac
     1261 cccgggttcc cgaagatagt ggtcatcccc agcttgcgga acaggtcgaa ggtcgcttcg
     1321 cgaacggtag gcatggccgc agcctatatg tgaacggctg agtacgggat cgatgggcgg
     1381 aggcagggcg gatgcccggg cctgtcagga ttcacccgtg cgcaaaactc tcgctttcgc
     1441 cctgatcctt cctgccctcg cccttccctt cgccggctgc agcgatggtc gcaagatcgc
     1501 gactcctccc gcctgtcttt ccgattcgtc gacctgggtc acggcgctcg cggcggcgcc
     1561 cgaccaggtc cgaatcagcg aagcgacacc gataagcgaa tgtctggtca aggatcaggt
     1621 ggccgcccag cagcaggagg tcggcaagac cgcggtcgag gcggcaaccc ggctcgccgc
     1681 gttctacaag ggcgctggca agaccggcaa gcaaactgac accggcacca aggtgtccgg
     1741 gggatcggcc agctccgagc aggcggccct catggccggc tatctggtcg gggccctgca
     1801 gaagggagcc ggggaaactg acggcattca cgcgacgttg gtcaaccggg tcgaagcggc
     1861 cgcctccaac ggactcgacg gcgccgacca gcaggtccag ggcgcctacc agaagggtca
     1921 tcaggccggc ctggattccg gctgacccga ccaacccttt ccgtcgggga ctgaaagact
     1981 gtccccgtaa acgagacggt gcggaaaggc agtgaagtga gcgagcaact ctggggtggc
     2041 gaaaccacca aggcgattga gaacttcccg gtttccggcg aaaccattcc gccttcgctg
     2101 gtcaagtggc tgggcctgat caaggccaac gcggcccggg ccaacgccga actcggcctg
     2161 ctcgacgccg acatcgccga tcgcatcgcc agggccgggg acgcggttgg gtccggcgag
     2221 cacgacgacc agttcccgat cgacatcttc cagactggtt ccggcacctc ctcgaacatg
     2281 aacaccaacg aggtgatcgc aacccttgcc ggcgagggag ttcacccgaa cgaccacgtc
     2341 aacatgggcc agtcctcgaa cgacgtgttt ccctccgccg ttcacctcgc cgcccttgat
     2401 caggtcaccc atgacctgct gccggccatg gaggaactgg cggtcgccct cgagacgaag
     2461 gccgaagagt tcaaggacgt ggtcaaggcc ggccgcaccc acctgatgga cgcggtgccg
     2521 gtcacgctcg gccaggaatt cggcggttac gcctcccagg tccgtcacgg catcgaccgg
     2581 gtccgtggca ccctcgaccg ggtcggccag attccactcg ggggcacggc aaccggaacc
     2641 ggtctcaaca cccacccgga gttctcggcc agggtgaggg cactgctcga agcggagacc
     2701 gggctggcga tctcgccgcc ccatgaccgg ttcgaggccc aggccaaccg cgactcgctg
     2761 gtcgagctct cgggagcttt gaaggtctac gcggtctcgc tgaacaagat cgccaacgac
     2821 ctggccctga tgggctccgg tccgcggacc ggcctcgccg aacttcatct gccggaactg
     2881 cagaagggct cctcgatcat gcccggcaag gtcaacccgg taattcccga agtcgtgatc
     2941 caggttgccg cccaggtcgt cggaaacgac atggcgataa ccatggccgg ctcccagggg
     3001 cagttcgaac tgaacgtgcg ggtgccgctg atcgcccgca acctgctcgg ctcgatccgc
     3061 atcctc
//
LOCUS       DAOTIP010000171         3064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37804_length_3064_cov_3.084746, whole genome shotgun sequence.
ACCESSION   DAOTIP010000171 DAOTIP010000000
VERSION     DAOTIP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37804_length_3064_cov_3.084746"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="ettA"
                     /locus_tag="P5138_04210"
     CDS             complement(<1..1419)
                     /gene="ettA"
                     /locus_tag="P5138_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRV59808.1"
                     /translation="MADYIFTMYQVSRVHPPDKEVLKDISLNFLPGAKIGILGPNGSG
                     KSSLLKIMAGTDTEFKGEARLREGATVGILEQEPHLDESRDVRGNVEDGVREIKDLLD
                     RFNELAANYSDETADEFAKLQAKIDAVDAWNLDTMLDTAMDALRLPPGDADVSKLSGG
                     EKRRVALCRLLLSKPDLLLLDEPTNHLDAESVAWLERHLEEYPGTVVAVTHDRYFLDN
                     VAGWILELDRGRGIPFEGNYSSWLEQKQARLAQEEKQESARAKTIASELEWVRENPKG
                     RRKKSKARLARYEELLAEEKNVRLDQVQIHIPAGDRLGNVVIDAEHLTKAFGDKLLIE
                     DLSFKLPPAGIVGIIGPNGAGKTTLFRMIVGDEEPDSGTIRLGDTVQLAYVDQSRDAL
                     DPEKTVWEEISDGDENITVGSNKMNSRAYVGGFNFKGSDQQRKVGVLSGGERNRVHLA
                     KLLKTGGNLLLLDEPTNDLDVDT"
     gene            1482..2108
                     /locus_tag="P5138_04215"
     CDS             1482..2108
                     /locus_tag="P5138_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRV59809.1"
                     /translation="MRVDLNFYLQSIVAVVVITDPLTRGIFFRQLTANEPERRVEFVR
                     RITLVVAITLFGSALIGRELLDVMGINLGAFGVAGGIVVTLMGFEMLFGGEPSRAQGG
                     EKSREVPESFDGSVVVPYAIPFIAGPGAITTVISIAAATDNGEGTVVALVAVAVAVIL
                     LPIGHLLVSKYVNLSEQAEGIVTKIGGLLITTIGIQLVLSGIQRFFEG"
     gene            2200..2535
                     /locus_tag="P5138_04220"
     CDS             2200..2535
                     /locus_tag="P5138_04220"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV59810.1"
                     /translation="MSQPLHEAKAELFRNLGHPVRVRALELLLEGPRPVHELLAEIEV
                     EPSNLSQQLAVLRRAGLVNSRREGSTVLYTLSSPEVAEMMDAARAVLTNVISGQQSLL
                     AELASEAGS"
     gene            2552..>3064
                     /locus_tag="P5138_04225"
     CDS             2552..>3064
                     /locus_tag="P5138_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRV59811.1"
                     /translation="MADSVKTRLRALLPQRSDLDALRRDPRRDLLAGLTVAVVALPLA
                     LAFGATSGLGAEAGIATAVVAGAVAAIFGGSNLQVSGPTGAMTVVLVPVVHQFGATGV
                     LFVGFLAGLLLIALGWAGMGRYVRFLPAPVVEGFTAGIAVVIALQQVPAALGVKAGDQ
                     ERVWAVAFQAV"
BASE COUNT          474 a          972 c         1012 g          606 t
ORIGIN      
        1 cgtgtcgacg tcgaggtcgt tggtcggctc gtcgagcagc agcaggttgc cgccggtctt
       61 cagcagcttg gccaggtgga cccggttgcg ctcgccaccg gagagcaccc cgaccttgcg
      121 ctgctggtcg gagcccttga agttgaagcc gccgacatag gcccgcgaat tcatcttgtt
      181 cgagccgacg gtgatgttct cgtcgccgtc ggagatctcc tcccagacgg tcttctccgg
      241 atcgagggca tcgcgggact gatccacata ggcgagctgg accgtgtcgc cgagccggat
      301 agtcccgctg tccggctcct cgtcgccgac gatcatccgg aacagggtgg tcttgccggc
      361 gccgttcggc ccgatgatgc cgacgatgcc ggcgggtggc agcttgaagg aaaggtcctc
      421 gatcagcagc ttgtcgccga aggccttggt caggtgttcg gcgtcgatca ccacgttgcc
      481 gagacggtcg ccggccggga tgtggatctg gacctggtcg agcctgacgt tcttctcctc
      541 ggccagcagt tcttcgtagc gggcgagacg ggccttggac ttcttgcgcc tgcccttcgg
      601 gttctcgcgg acccattcga gttcggaggc gatcgtcttc gcccgggccg actcctgctt
      661 ttcctcctgg gcgaggcggg cctgcttctg ctcgagccag gagctgtagt tcccctcgaa
      721 ggggatgccg cggccgcggt cgagctcgag gatccagccg gccacgttgt cgaggaaata
      781 gcgatcgtga gtcacagcga cgacggtgcc cggatattcc tccaggtgcc gttccagcca
      841 ggccaccgac tcggcgtcga ggtggttggt cggctcgtcg agcaacaaca ggtccggctt
      901 ggaaagcagc aggcggcaga gcgcgacccg gcgcttctcg ccgccggaga gcttcgagac
      961 gtcggcatcg cccggcggca ggcgcaacgc gtccatcgcc gtgtcgagca tcgtgtcgag
     1021 gttccaggcg tcgaccgcgt cgatcttcgc ctgcagcttc gcgaactcgt cggcggtctc
     1081 gtccgagtag ttggcggcca gttcgttgaa gcggtcgagc aggtccttga tctctcggac
     1141 cccgtcctcg acgttcccgc gcacgtccct gctctcgtcg agatgcggct cctgctcgag
     1201 aatgccgacc gtggccccct cgcgcagcct ggcttcgccc ttgaactcgg tgtctgtccc
     1261 ggccatgatc ttcagcagcg aggacttgcc ggatccgttc ggtccgagga tgccgatctt
     1321 ggctccaggc aggaagttca gcgagatgtc tttgaggacc tccttgtcgg gcggatgaac
     1381 ccgggagacc tggtacatgg tgaagatgta gtcagccatc gccgaagaga ctatccagcc
     1441 ggggttgccg accggggcgc tctctacgct gcgcggcagg gatgagggtc gacctcaact
     1501 tctacctgca gtcgatcgtc gcggtagtgg taatcaccga tccgcttacc cggggcatct
     1561 tcttccggca gctcactgcc aacgaacccg aacggcgggt cgaattcgtc cggcggataa
     1621 ccctcgtcgt ggccatcact ctcttcggct cggccctgat cggtcgcgag ctgctcgacg
     1681 tgatgggaat caaccttggg gcattcggcg tggccggcgg gatcgtggtc acgctgatgg
     1741 ggttcgagat gcttttcggc ggtgagccga gccgggctca gggcggcgag aaatcccggg
     1801 aggtaccgga aagctttgac ggcagcgtcg tcgtgccgta cgcgatccct ttcatcgccg
     1861 gtcccggtgc gatcaccacg gtgatctcga tcgccgccgc caccgacaac ggcgagggga
     1921 ccgtcgtcgc cctggttgcg gtcgccgtag cagtgatcct gctgccgatc ggtcacctgc
     1981 tggtctcgaa gtacgtgaat ctctccgagc aggcagaagg gatcgtcaca aagatcggcg
     2041 ggctgctgat caccacgatc ggcatccagt tggtgctcag cggcatccag cggttcttcg
     2101 agggctgagc ctcccgggcg tggtccggac ccggtcaagt gttcaaatct cacagatttc
     2161 gcaattgaag aattacgcaa cttgtgaaac catgtcctca tgagtcagcc gctccatgaa
     2221 gccaaagccg aactgtttcg caatctcggc catccggtcc gggtgcgggc cctggaactg
     2281 ctgctggaag gtccccggcc ggtacacgaa ctcctggccg agattgaagt cgaaccttct
     2341 aacctctcgc aacagctcgc agtgctgcgc cgggccggtc tcgtcaactc gaggcgggaa
     2401 ggttcgacgg tgctctacac gctgagcagc cccgaggtcg cggagatgat ggacgcggcc
     2461 cgggccgttc tcacgaatgt gatttccggc cagcagtcgc tgcttgcgga gctggcctcc
     2521 gaagcaggct cctgagcgtc tggaagtcaa ggtggctgat tcggtcaaga cacgcctgcg
     2581 tgcgctcctg cctcaaaggt ccgacctcga cgcattgagg cgagaccccc ggcgcgacct
     2641 gctcgccgga ttgacagttg cggtcgtcgc cttgccactt gccctggcct ttggcgccac
     2701 atcggggctg ggcgccgaag cagggatagc caccgccgtc gtcgccggcg cagtggcggc
     2761 gatcttcggc ggctccaatc tccaggtctc agggccgacc ggcgcgatga cggtggtcct
     2821 ggttccagtg gtgcatcagt tcggagcgac cggagtcctc ttcgtcggtt tcctagccgg
     2881 gcttcttctc atcgcactcg gctgggccgg tatggggcga tacgtgcgct tcctcccggc
     2941 accggtggtc gagggattca ccgccggcat cgccgtggtg atcgctctcc agcaggtgcc
     3001 ggcggcactc ggagtgaaag cgggagacca ggaacgcgta tgggctgtgg cattccaggc
     3061 cgtc
//
LOCUS       DAOTIP010000172         3060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37862_length_3060_cov_2.842596, whole genome shotgun sequence.
ACCESSION   DAOTIP010000172 DAOTIP010000000
VERSION     DAOTIP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37862_length_3060_cov_2.842596"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="P5138_04230"
     CDS             <1..1129
                     /locus_tag="P5138_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59812.1"
                     /translation="YTVALPFSVAVFISALLQRRWNRIWVGLVPIALYLAWRALGNVG
                     ATDTAAGGGDLLNILLLPAWSFGGISGVLGSWSGLSFDFVRSGAVQPGTGIGPVLAAA
                     ALVALGWRIATRGADFRLWTVIAVALALFAAQTLAVGGIERYPEMPRYLYPGLIVVML
                     VVVEALRGFAWSRATFVALWLITAISLLTSFSLLRQQSEGREWKAEQTRIEVTAVSLL
                     ADTPSPPPVSRQPRRMVTDDFDPKTAGDYGDLGYDPATLSDRPVEIGNGVDRFLYESL
                     KPGLQATTVPFVKADCEPLKAINGRYRISLPRNGAVITSTREAKLTIGRFGSGRSLKI
                     GTVTPNSPQLLKLRYDGEPTPWFIKSKTPGVSACPFDSGDL"
     gene            1194..2267
                     /locus_tag="P5138_04235"
     CDS             1194..2267
                     /locus_tag="P5138_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV59813.1"
                     /translation="MPETISFARGAPSPDLIPVDAVRVATAKALEDDWQKALSYGVGI
                     GHPGLCAWIADRHGIPTEQVMTTNGSLEAGAMLFRHLLSPGDRVIVEQPSYDRTLLLL
                     EQLQVEFVPVALEADGIDVDAVERAVKHAPVKLAHIIPNFHNPAGCTLSAEKRERLVA
                     LAAEHDFTIFEDDPYRELPFGEDPPPTMLSIDQADKVIHASSFSKTVSPGVRVGYLAG
                     PEHEIARLTKIANENYISPNMLSGSLVLELCQSGVLDDNIEKCKVALQERRDALVDAL
                     GKHIPEASFVLPGGGYFLWADLDEGRNTVELAEKAKEAGAPFIPGTEFTLDGGWNSLR
                     FSFASVPPEHMDEGVARIASCLD"
     gene            complement(2264..2968)
                     /locus_tag="P5138_04240"
     CDS             complement(2264..2968)
                     /locus_tag="P5138_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRV59814.1"
                     /translation="MADATVAEEEYLQTIFWLMEAGLPITGANIAKAMQLSPPTVHEM
                     IKRLERDGYVTRSADKILHLTDEGRAEAEQITRRHRMIERFLTDVLGIPWDEVHEEAE
                     RLEHAMSPVLEERMRAAIGEANTCPHGHPIDGVREQGAPLADVDLGAKVVVLRFENEA
                     ESLLHYLMDIGLRPGLEGTVVAKDDDTVLIESADGKHEVTRTVAETVTVRADPAPPPR
                     AALPDQLVISDDRYGR"
BASE COUNT          486 a         1067 c          969 g          538 t
ORIGIN      
        1 ctacacggtc gcgctgccgt tctcggtggc cgtcttcatc tccgctctgc ttcaacgcag
       61 atggaaccgc atctgggtcg gcctggttcc gatcgcgctc tatctggcct ggcgggcact
      121 gggcaacgtc ggtgcgaccg acaccgcggc cggcggcggt gacctgctga acatcctgct
      181 gctcccggcc tggagcttcg gagggatctc cggcgtgctc gggtcctggt ccgggctcag
      241 cttcgacttc gtccgttccg gcgccgtcca gccgggcacg ggcataggtc cggtgctcgc
      301 ggcggccgcg ctggtggccc tcggttggcg aatcgctacc cggggggccg acttccggct
      361 ctggacggtg atcgctgtcg cgctcgccct gtttgccgcc cagaccctcg ctgtgggcgg
      421 gatcgagcgt tacccggaga tgccccgcta cctgtatccg gggctgatcg tggtcatgtt
      481 ggtggtggtc gaggccctgc gcggattcgc ctggtcccgg gccaccttcg tagcgctctg
      541 gctgatcacg gcgatcagcc tgctgacctc cttttccctg ctcaggcagc agtcggaggg
      601 ccgggaatgg aaggcggaac agacccggat cgaagtcacc gcggtttcgc tcctcgccga
      661 caccccttct cccccgcctg tgtcccgtca gccgcgccgg atggtgaccg acgattttga
      721 tccgaaaacg gccggcgact acggcgacct cggctatgac ccggcgaccc tgtccgaccg
      781 cccggtggag atcggcaacg gtgtcgaccg cttcctctac gaatcgctga agccgggcct
      841 tcaggccacg accgtgccct tcgtcaaggc cgactgcgag ccgctcaagg caatcaacgg
      901 tcgctacagg atctcactgc cccgcaacgg cgcggtcatc accagcaccc gcgaagcgaa
      961 gctcacgatc ggacgcttcg ggtcgggacg ctcgctaaaa atcggcaccg tgaccccgaa
     1021 ctcgccccag ctgctcaagc tccgctacga cggggaaccg acaccctggt tcatcaagag
     1081 caagactccc ggcgtttccg cctgcccgtt cgactccggc gacctctgag cgaacggccg
     1141 cccggccatc ggtaacatcg cctctcccat tccacgaccg ctcggagagc gagatgccag
     1201 agacgatcag ctttgccaga ggtgccccca gcccggacct gattccggtc gacgcggtcc
     1261 gcgtcgctac cgcgaaggcg ctggaggacg attggcagaa ggcgctctcc tacggtgtcg
     1321 ggatcggaca cccgggactc tgtgcctgga tcgccgaccg ccacgggatc ccgacggaac
     1381 aggtgatgac caccaacggc tcgctggaag ccggggcgat gctcttccgc cacctgctct
     1441 cccccggaga ccgggtgatt gtggagcaac cgagctacga ccgcaccttg ctgctgctcg
     1501 agcaactcca ggtcgagttc gtgccggtcg cgctcgaagc agacggcatt gacgtcgacg
     1561 cggtcgagag ggccgtgaaa cacgctccgg tcaagctcgc gcacataatc cccaacttcc
     1621 acaatccggc cggctgcacc ctttcggccg aaaagcgcga gcggctcgtc gccctggccg
     1681 cggagcatga cttcacgatc ttcgaggacg acccgtaccg ggagctgccc ttcggtgagg
     1741 accccccgcc gaccatgctc tcgatcgatc aggccgacaa ggtgatccac gcctcctcct
     1801 tctcgaagac ggtgagcccg ggcgtgaggg tcggatacct ggccggtccc gaacatgaga
     1861 tcgccaggct gacgaagatc gcaaacgaga actacatctc cccgaacatg ctctccgggt
     1921 cgctggtgct ggagctctgc cagtccggcg tcctggacga caacatcgag aagtgcaagg
     1981 tcgccctcca ggagaggcgc gacgccctgg tcgacgcgct cggaaagcac atccccgaag
     2041 cgagtttcgt cctgcccggc ggcggctact tcctctgggc cgacctcgac gaaggacgca
     2101 acacggtcga actcgcagag aaagccaagg aagccggcgc cccgttcatc cccggaaccg
     2161 agttcacgct cgacggcggc tggaactcgc tccgcttctc cttcgcctcg gtccccccgg
     2221 agcacatgga cgaaggcgtc gcccgcatcg cctcctgcct ggattagcgg ccgtagcggt
     2281 cgtccgagat gaccagctgg tccgggaggg ctgccctcgg gggcggggcc gggtcggctc
     2341 tgacggtgac cgtttcggcg accgtgcggg tcacctcatg cttgccgtcg gctgactcga
     2401 tcaggacggt gtcgtcgtcc ttggccacga ccgttccctc gaggcccgga cgaaggccga
     2461 tgtccatcag gtagtggagc aggctctcgg cctcgttctc gaaccggagc accacgacct
     2521 tggccccgag gtcgacgtcg gccagcggag cgccctgctc gcggaccccg tcgatcggat
     2581 ggccatgcgg acaggtgttg gcctccccga tcgcggcccg catccgttcc tcgagcaccg
     2641 gtgacatcgc atgctcgagc cgttcggcct cctcgtgaac ctcgtcccat gggatgccga
     2701 gcacgtcggt gaggaaacgc tcgatcatcc ggtgacggcg ggtgatctgt tccgcctcgg
     2761 cccggccttc atcggtcagg tggaggatct tgtcggcgct acgggtcacg tagccgtccc
     2821 gttcgagccg cttgatcatc tcgtggacgg tcggcggcga gagctgcatc gccttggcga
     2881 tgttggcgcc ggtgatcggc aatccggctt ccatcagcca gaagatcgtc tgtagatatt
     2941 cctcttcggc aaccgtcgcg tcagccatgg actgatccta gatgttcggg accagttgcc
     3001 gttcgagcgc cgctcaggcg cccatcagcg aggtgaccac ggcttcatag aagccggcgc
//
LOCUS       DAOTIP010000173         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_37981_length_3052_cov_2.817150, whole genome shotgun sequence.
ACCESSION   DAOTIP010000173 DAOTIP010000000
VERSION     DAOTIP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37981_length_3052_cov_2.817150"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /gene="hisI"
                     /locus_tag="P5138_04245"
     CDS             complement(<1..355)
                     /gene="hisI"
                     /locus_tag="P5138_04245"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRV59815.1"
                     /translation="MTAIEVKFDRDGLVPCIAQDADTGEVLTLAYMNEESLQMTRETG
                     LVTYFSRSRQEIWQKGEESGNVQKVRQLRVDCDGDALVALVEPAGPACHTGERTCFFR
                     DLETGNTPEPVTGEAL"
     gene            complement(357..803)
                     /locus_tag="P5138_04250"
     CDS             complement(357..803)
                     /locus_tag="P5138_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59816.1"
                     /translation="MLRKAAICALMATTLTCTALVAAGCGGSDDSTSEEDNQAITDLV
                     SKLNQATRDKDASEFCLIMQPSAVDETFHDIDRCVSETKPILQAAGKQPVLKVESIEV
                     NGDIATVTFAGSSGNEAKFVREDGQWYVPLSQSEITTNPDSAGNGG"
     gene            complement(1297..2250)
                     /gene="era"
                     /locus_tag="P5138_04255"
     CDS             complement(1297..2250)
                     /gene="era"
                     /locus_tag="P5138_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRV59817.1"
                     /translation="MSELPDPPEPESGALSGTGTRSGFVGLAGRPNVGKSTLVNAIVG
                     TKVAISSVRPQTTRRAIRGVATDPEAGTQLVLVDLPGVQRPRDELTSRMQKTVEGELA
                     GADVALLVINGEEGVGPGDRFIAETLLKARQGEGSLPIICAVNKADRLGRNIVPVLVA
                     ASELAGVDEVFPVSALTGEGVPELAAHLASIVPAGPYLYPPEDRSDQPRELLLAELIR
                     GQSLIRTRDEIPHSVEVRVTRIDQREDGLWLVDAEIWVESDSQKKILIGKKGFKVGEI
                     GTGARKDIENELGGKVHLDLQVKVKRKWRRDQDMLDRLGID"
     gene            complement(2243..2641)
                     /gene="ybeY"
                     /locus_tag="P5138_04260"
     CDS             complement(2243..2641)
                     /gene="ybeY"
                     /locus_tag="P5138_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRV59818.1"
                     /translation="MNFDLDDVPDRFQPAVIATLEAAGVGEGHLAVEIVDESRIRELN
                     RKHRDKEGPTDVLSFPVDEEAGPGPVELGDVILCPEYCVNDVEAVVHGVLHLCGYDHE
                     TDEGEMLDLQDRITTGLGIEPKMTVEADGE"
     gene            complement(2638..>3052)
                     /locus_tag="P5138_04265"
     CDS             complement(2638..>3052)
                     /locus_tag="P5138_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRV59819.1"
                     /translation="PYLRPLFDALYDMLDPDRVNGYFDRGVIEVAPLAFMRGRTLNDA
                     FVILDEAQNTTPEQMKMFLTRLGFNSKMVVTGDVTQIDLPRDSKSGLVVVREILEGIE
                     DVKFVRFGGKDVVRHRLVQRIVAAYGEHAERESDS"
BASE COUNT          499 a         1011 c          969 g          573 t
ORIGIN      
        1 caagggcctc gccggtcacc ggttccggtg tattccccgt ctcgaggtca cgaaagaagc
       61 aggtgcgctc gccggtgtgg caggccgggc cggcgggctc gaccagggcc acgagtgcgt
      121 ctccgtcgca gtcgaccctt agttgccgga ccttctggac gttgcccgac tcctcgccct
      181 tctgccagat ctcctgccgc gaccggctga aatacgtgac cagaccggtc tcccgggtca
      241 tctgaaggga ctcctcgttc atgtaggcaa gggtcagcac ctcgcccgta tccgcatcct
      301 gggcgatgca cggaacgagt ccgtcccgat cgaacttcac ctcgattgcc gtcacttcag
      361 cccccgttcc cggccgaatc cgggttggtg gtgatttcgc tctggctgag aggcacgtac
      421 cactggccgt cctcgcgcac gaactttgcc tcgttgccgc tgctgccggc gaaggtgacg
      481 gtggcgatgt cgccgttcac ttcgatgctc tcgaccttca ggaccggctg tttgccggcc
      541 gcctggagga tcggcttcgt ctcactcacg cagcgatcta tgtcgtgaaa ggtctcgtcg
      601 accgcgctgg gctgcatgat caggcagaac tcggaggcat ccttgtcccg ggtcgcctgg
      661 ttcagcttgg agaccagatc ggtgatcgcc tggttgtctt cttccgaagt cgaatcatcc
      721 gagccgccgc agcccgccgc aacgagcgcg gtgcaagtca gggtcgtcgc catcagggcg
      781 catattgcgg cttttcgcag catgcgccca atcctacgga agcaggggga aactccgaca
      841 agacgccaca aggtctcccc gagccaacct cccgagctct tgcacagggt ccggtggtgt
      901 acctccgaaa ccgtcgcgct tcgcgctccc catccagagg ttccggaatc acaccaccga
      961 accctgcttg acgtctcggg gaactgatct acgagacttg aagcccgatt cgggggtacg
     1021 gtttcgggac ctctggatgg ggagcgcagc gacggtcccg gggccgtacc cccggagcgg
     1081 gcgccagcgt gacggatcag tcgatcgcgg ctacccgaag gtcggcgaga tgcgccagaa
     1141 ccgagcttcg gcgggtggtt cgtctcggag accctaggcg cggctggcaa ggagacaggc
     1201 cggaaatggc ggcgatagta cgcgggtacg tgagctgtcg tttccggccg ccgacgaagc
     1261 caggcgtgca taggggcccg agacgggctg cccgggctag tcgatgccga ggcggtcgag
     1321 catgtcttgg tcgcggcgcc atttgcgctt caccttgacc tgaaggtcga ggtggacctt
     1381 tcctcccaat tcgttttcga tgtccttgcg ggcgccggtt ccgatctcgc cgaccttgaa
     1441 gcccttcttg ccgatcagga tcttcttctg cgagtcggat tcgacccaga tttcggcgtc
     1501 gaccagccac agcccatcct cgcgctggtc gatccgagtg acccggacct cgaccgaatg
     1561 ggggatttcg tcgcgggtac ggatcaggga ctgaccgcgg atcagttcgg ccagcagcag
     1621 ttctcgcggc tggtcggaac ggtcctccgg cggatacagg taagggcccg ctggaacgat
     1681 cgaagccagg tgcgccgcaa gctccggcac cccctctccg gtcagggcac ttaccgggaa
     1741 gacctcgtcc acgccggcga gttcggaggc cgcgaccagc accggcacga tgttccgtcc
     1801 gagacggtcg gctttgttga ccgcgcagat gatcggcagg gatccctcac cctgcctggc
     1861 cttgagcagc gtctcggcga tgaaccggtc cccggggccg acaccttcct cgccgtttat
     1921 gaccagcaga gccacatcgg cgccggccag ttccccttcc acggtcttct gcatcctgct
     1981 ggtcagctcg tcacggggcc tctggactcc gggcaggtcg acgaggacca gctgggtgcc
     2041 ggcttccggg tcggtggcga cgccgcgaat cgccctgcgc gtggtctgtg gcctgaccga
     2101 cgagatcgcc accttggtgc caacgatcgc attgaccagg gtcgacttgc cgacgttcgg
     2161 acgaccggcc aggccgacaa aaccggaacg ggtgccggtg ccggacaatg ccccggattc
     2221 cggctcggga ggatcgggaa gttcactcac catctgcctc cacggtcatc ttcggctcga
     2281 tcccgaggcc ggtcgtgatc cggtcctgca ggtcgagcat ctccccttcg tcggtctcgt
     2341 ggtcgtagcc acagaggtgg agaaccccgt ggaccaccgc ttcgacatcg tttacgcagt
     2401 attcggggca gaggatcacg tcaccgagtt cgaccgggcc gggcccggcc tcctcgtcaa
     2461 ccggaaaaga gagcacgtcg gtcggcccct ccttgtcgcg gtgcttgcgg ttcagttccc
     2521 ggatccggga ctcgtccacg atttcgacgg cgagatgccc ctcacccact ccggccgctt
     2581 ccagggtcgc gatcaccgcc ggctggaacc ggtcgggcac atcgtcgagg tcgaagttca
     2641 actgtcgctt tctcgctcgg cgtgctcgcc gtatgcggcg acgatccgct ggaccagccg
     2701 gtgccggacc acgtccttgc cgccgaaccg cacgaacttc acgtcctcga tcccttcgag
     2761 gatctcccga accacgacca gacccgactt gctgtcccgg ggaaggtcga tctgggtgac
     2821 gtcaccggtg accaccatct tcgagttgaa cccgagccgg gtgagaaaca tcttcatctg
     2881 ctcgggcgtc gtgttctggg cctcgtcgag gatcacgaaa gcgtcgttca gggtgcggcc
     2941 acgcatgaag gcgagcggag caacttcgat cactccgcgg tcgaagtagc cgttgacccg
     3001 atccggatcg agcatgtcgt agagcgcgtc gaagagcggg cgaaggtacg ga
//
LOCUS       DAOTIP010000174         3047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38074_length_3047_cov_3.301805, whole genome shotgun sequence.
ACCESSION   DAOTIP010000174 DAOTIP010000000
VERSION     DAOTIP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38074_length_3047_cov_3.301805"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="P5138_04270"
     CDS             complement(<1..730)
                     /locus_tag="P5138_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRV59820.1"
                     /translation="MSSNSFGSKDTIESGGESYEIFRLDALQEKFDVARLPYSIKVLL
                     ENVLRLEDGVSVSAADVEAIASWDAKAEPSVEIPFQPARVLMQDFTGVPAVVDLAAMR
                     DAMADIGGDVSAINPLVDVDLVIDHSVQVDAFGNGMAFEINAEKEFERNLERYEFLKW
                     GQGSFNNFRVVPPATGICHQVNLEYIAQVVYSRENDGVLQAYPDTLVGTDSHTTMVNG
                     LGVLGWGVGGIEAEAAMLGQPISML"
     gene            complement(762..1562)
                     /locus_tag="P5138_04275"
     CDS             complement(762..1562)
                     /locus_tag="P5138_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59821.1"
                     /translation="MEIAGKKVLLTGATGGIGRTIAGTLAAAGANLILSARSAEALEE
                     LAGSLPGSGHSVIVSDLGTPGAAGKLAASAGEVDVLVANAALPSTGRVTDLTDEQVAR
                     MLRINLESPILLAQALLPGMLERGRGKLVMIGSLAGKAGSPRSSIYNATKFGLRGFAF
                     GLAADLNDTPVSLTLVAPGFVREAGMFADSGADAPPGLGTTTPGKVADAVLRAVDSDR
                     IEITVAPLIQRTMAHVGLMSPHISHRVQSGPAGQNAADSLASGQTEKR"
     regulatory      1723..1868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2113..2472
                     /locus_tag="P5138_04280"
     CDS             2113..2472
                     /locus_tag="P5138_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59822.1"
                     /translation="MVKKAIAAANRIRLKPYIYGGGHSMSRKLQKGYDCSGSVSYALR
                     AAGLIGYPMPSGSFVRWKQPGAGKWITTYANGGHMYMVIAGLSFDTSNMSSTGGNRWS
                     TAIRSSRGFSARHPGNF"
     gene            2613..>3047
                     /locus_tag="P5138_04285"
     CDS             2613..>3047
                     /locus_tag="P5138_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV59823.1"
                     /translation="MEIRKLGSEGLEVSAIGLGCMGMSEFYGEGDEAESIATIHRALE
                     VGVNFLDTADMYGPWTNEELVGRAIADRREKVVLATKFGIMRGDDPRDRHVNGRPEYV
                     RESIDGSLKRLGVDHVDLYYQHRVDQSVPIEETVGAMAELVEA"
BASE COUNT          549 a          993 c          990 g          515 t
ORIGIN      
        1 tcagcatcga gatcggctgg ccgagcatcg ccgcctcggc ctcgatgccg cccacgcccc
       61 agccgaggac cccgagcccg ttgaccatgg tcgtgtggga gtcggtgccg accagggtgt
      121 ccgggtaggc ctggagcacg ccgtcgttct cgcgggagta gacgacctgg gcgatgtatt
      181 cgaggttgac ctggtgacag atgccggtag ccgggggtac cacccggaag ttattgaagg
      241 agccctgccc ccacttgagg aactcgtaac gttcgaggtt ccgctcgaat tccttctcgg
      301 cgttgatctc gaaggccatc ccgttgccga aggcatcgac ctgaaccgaa tggtcgatca
      361 cgaggtcgac gtcgaccagc gggttgatcg ccgatacgtc accaccgatg tcggccatcg
      421 cgtcacgcat cgcggccagg tcgaccacgg ccggaacacc ggtgaagtcc tgcatcagga
      481 cccgggccgg ctggaagggg atctcgaccg aaggctcggc cttggcatcc caggaagcaa
      541 tcgcctccac atcggcagcg ctgaccgaga ccccgtcctc gagacgcagc acgttctcga
      601 gcaggacctt tatcgagtag ggcagcctgg cgacatcgaa tttctcctgc agggcgtcga
      661 ggcggaagat ctcgtagctc tccccgccgg actcgatggt gtctttggaa ccgaatgaat
      721 tgctgctcat gttctttctt ctctcgatcg aggggtgagg gtcaacgttt ttcggtctgg
      781 ccgctggcga gggagtcggc ggcattctga ccggccgggc ccgactggac ccgatgggaa
      841 atatggggac tcatcaggcc gacatgggcc atggtccgct gtatcagggg agcgaccgtg
      901 atctcgatcc ggtcggaatc gaccgccctg agaaccgcgt ccgcgacctt tcccggggta
      961 gttgtgccga ggccaggagg ggcatcggcg ccggagtcgg cgaacatgcc cgcctcgcgg
     1021 acgaatcctg gagcgaccag ggtaaggctc accggggtgt cgttcaggtc ggcggccagc
     1081 ccgaaggcga acccccgcag gccgaacttg gtcgcgttat agatggagga acggggactg
     1141 cccgccttgc cggccagcga gccgatcatc accagcttgc cgcgaccccg ctccagcatg
     1201 cccggcagta gcgcctgggc caggaggatc ggggactcga ggttgatgcg cagcatgcgg
     1261 gcgacctgct cgtcggtcag gtcggtcacc cggccggtgc tcggcagagc tgcgttggcg
     1321 accagcacat cgacctcccc tgcgctcgcc gccagtttcc cggcggctcc cggggttccc
     1381 aggtcactga cgatcacgga gtggccgctc ccgggcagag agccggccag ttcttccagg
     1441 gcctcggccg aacgggccga gaggatgagg ttggcgccgg cggcggcgag tgtgccggcg
     1501 atcgtgcggc caatacctcc ggtcgcgccc gtcagaagca ccttcttgcc agcgatttcc
     1561 accagtcaaa cctagcaccg cgattgccct tagcctcccc aaaggccgct ttgggggcgg
     1621 ttttcggagg acaccgtgtt tccggaattt gcagggatta acaaagggat tcgggggttt
     1681 gttagcgaat gacatgcaga tcgtctatga tcccgcaagt tcataaccga gacgccgaat
     1741 cccgaatcac tctgtgaccg ggagcggggg aaccgagatt ctggggcgaa cccaccaaac
     1801 aaggtggggg cgcggctcct aaagcgccag cccgacagct aaccccgtag gccgaccaag
     1861 gagttcggta tcaacccgtc gcacaaaatc gagtcgcact ttgctttcaa tgctttccgg
     1921 gctttcgcag cagccgccgc gctggtcgcg ctgagcctgg tcgccctctt ctccctcgcc
     1981 agcccggccc aggccgccgg ttgcaaggac gcgtcgagtg gcggactcac cctcgccccc
     2041 accacctgca agccgatcaa gaaggcccgg ctgatccgcg gcaaggccta cgccccggcc
     2101 accgccccgg ccgtggtcaa gaaggcgatc gccgcagcca accggatccg cctcaagccg
     2161 tacatctacg gtggcggcca ctcgatgagc cgcaagctcc agaagggcta cgactgctcc
     2221 ggctcggtca gctacgcgct ccgcgccgcc ggcctgatcg gctacccgat gccttccggc
     2281 agcttcgtgc gctggaagca gcccggagcc ggaaagtgga tcacgaccta cgccaatggt
     2341 ggccacatgt acatggtcat cgccggcctc tccttcgaca cttcgaacat gagctccacc
     2401 ggcggcaacc gctggagcac cgcgatccgg tcttcccggg gcttcagcgc caggcacccc
     2461 ggcaacttct aggacctgcc gacacagcag attggaaaac gccccggttc cggggcgttt
     2521 ttctttgcag caggcccgaa ccatggcgtt ggcgccgaaa gccggttcaa accttcccgc
     2581 gggccggtcc gccccggttc tatgatcggc tgatggagat tcgaaagctg ggcagcgagg
     2641 ggctcgaggt atccgcgatc gggctgggct gcatgggtat gtccgagttc tacggtgagg
     2701 gcgacgaagc ggaatcgatc gcaacgatcc accgcgccct ggaagtcgga gtcaacttcc
     2761 tcgacaccgc cgacatgtac gggccgtgga ccaacgaaga gctggtcggc cgagcgatcg
     2821 ccgaccgccg ggagaaggtg gtgctggcga caaagttcgg gatcatgcgt ggcgacgatc
     2881 cccgcgaccg tcacgtcaat ggccggcctg aatacgtgcg ggagtcgatc gacggttcgc
     2941 tgaagcggct cggagtcgac cacgtcgacc tctattacca gcaccgggtg gatcagagcg
     3001 tgccaatcga ggaaaccgtc ggcgccatgg ccgagctggt cgaggcc
//
LOCUS       DAOTIP010000175         3041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38166_length_3041_cov_3.608841, whole genome shotgun sequence.
ACCESSION   DAOTIP010000175 DAOTIP010000000
VERSION     DAOTIP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38166_length_3041_cov_3.608841"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="P5138_04290"
     CDS             complement(<1..380)
                     /locus_tag="P5138_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV59824.1"
                     /translation="MKATDLLQELIRFNTVNPPGNEGPALEHIRDFLEPAGWHCKFLS
                     KVPERPNLVARLRGAEPGPNLALISHVDTVPANREEWSRDPWSGDLVDGYIWGRGALD
                     MKDQVAAEVAACVNLAESGWRPARG"
     gene            437..1510
                     /locus_tag="P5138_04295"
     CDS             437..1510
                     /locus_tag="P5138_04295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV59825.1"
                     /translation="MSSLGKILITGGAGFVGSSLAISLARSHPDSHVIALDNLYRRGS
                     ELNLDRLADAGVEFVKGDVREPADLAPFSDIDALIECSAEPSVMSGTDGDTSYIYETN
                     LTGAYNCLELARKSGCQFIFLSTSRVYPLAGLRRADLIETGTRFELAEMQTAPGWTAA
                     GVAETMPLEGARTFYGTTKLSAEFLAAEFAAQFGVPVVINRCGVIAGPWQMGKVDQGV
                     FTHWMLAHEYEKPLSYIGFGGTGKQVRDLLHVDDLVELIEMQLADPAHWSGQTFNVGG
                     GREVSLSLLEATGICAGLTGHELEIAEVSEDRDGDVPLYISDCSRLFEHTSWRPGRDA
                     KCVLADIHEWIESDRDRVGEVLG"
     gene            1507..2238
                     /locus_tag="P5138_04300"
     CDS             1507..2238
                     /locus_tag="P5138_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV59826.1"
                     /translation="MKLSVVIPARDEEGSIMETVNGAVEVMERESIDYEILVIDDGSK
                     DSTRRLVEEAGESNPKIRCRPSPYSGGFGLAVRAGLEEFQGDGVAIMMADASDDPEDL
                     VMYHHILDEGWDCAFGSRFMPGAVVTDYPRLKLIVNRMANAFVNVLFRLGYNDTTNAF
                     KAYRREVIETIRPLLSKHFNLTVEMPLKAVTRGHSFKVVPTSWANRTSGEAKLAMREM
                     GSRYLFIVLYIWLEYHLSRGDYRKD"
     gene            complement(2223..>3041)
                     /locus_tag="P5138_04305"
     CDS             complement(2223..>3041)
                     /locus_tag="P5138_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59827.1"
                     /translation="RGRVPPEFLVGAAIGVSFWVLSGLNFIPGREFVSSRYQYPSVVM
                     LLMILGGAFAGYRPAPRTVRVIAAVAIFAIVLNAATLVFAFHDRYKKYEQKNLISFSA
                     FDLARRTVSPDFEVGAGVDDSARVDAASYFKAIDRYGSPALSEAQAEEASDENRDRLD
                     QLLVLGLPVQPVPATRVIPIRDRCRELAAHSEASGKIRIDPGLSWISAEKDVLIRLNR
                     FGTGRGAAAWSASAGKPIGYRIPRDNSDLPWHIGFQGAGRVTVCPARADSQSLR"
BASE COUNT          557 a          974 c          976 g          534 t
ORIGIN      
        1 cccctggcgg gccgccagcc ggactcggcc aggttcacgc aggcggcgac ctcggccgcc
       61 acctgatcct tcatgtcgag ggcgccgcgg ccccagatgt agccgtcaac caggtcgccg
      121 gaccaggggt cgcgggacca ctcctcccga ttcgcgggca ccgtgtcgac gtggctgatc
      181 agggccaggt tcggaccggg ctcggcaccc ctcagtcggg ccaccaagtt cggtcgctcc
      241 ggcacctttg acaggaactt gcagtgccag cctgccggtt ccagaaaatc ccggatgtgc
      301 tcaagcgccg gtccttcgtt tccgggtgga ttgacggtgt tgaaccggat cagttcctga
      361 agcaggtcgg tcgccttcat tcgcggagta tcccaacccg ccctggccgg gaatccaaat
      421 aggctccttc gatcgaatga gcagcctggg gaagatcctg attaccggcg gagcggggtt
      481 cgttggctcc agcctggcca tctcgctcgc ccgatcgcac ccggattccc acgtgatcgc
      541 gctggacaac ctgtaccggc gcgggtcgga actgaacctg gaccgactgg ccgatgccgg
      601 agttgaattc gtgaagggag acgtcaggga gccagccgat ctcgctccct tttccgacat
      661 cgacgccctg atcgaatgct cggccgaacc atccgtaatg tcaggcaccg acggtgacac
      721 ctcgtacatc tacgagacca acctgaccgg cgcctacaac tgcctcgaac tggcgaggaa
      781 gagcggctgc cagttcattt tcctttcgac cagtcgggtc tacccgctgg ccggtctgag
      841 acgggccgac ctgatcgaga ccgggacacg attcgaactg gccgagatgc agacggcccc
      901 gggctggacc gcagccgggg ttgccgagac gatgccgctg gagggtgccc gcaccttcta
      961 cggaaccacc aaactctcgg ccgagttcct cgccgctgaa tttgcggccc agttcggtgt
     1021 gccggtcgtg atcaaccgct gtggcgtgat cgccgggccc tggcagatgg gcaaggtcga
     1081 ccagggcgtt ttcacccact ggatgctcgc ccacgaatac gagaagccgc tctcctacat
     1141 cggcttcggc gggaccggca agcaggtgcg cgacctgctc catgtcgatg acctggtcga
     1201 gctgatcgag atgcaattgg ccgaccccgc gcactggtcc ggacagacct tcaacgtcgg
     1261 cggtggccgg gaagtcagcc tctcgcttct ggaagcaacc ggtatctgcg ccgggctgac
     1321 cggtcacgag cttgagattg ctgaggtctc cgaggatcgg gacggggacg tgccgctcta
     1381 catctcggac tgctcccgcc tgttcgaaca cacttcctgg cgccccggcc gtgatgcgaa
     1441 atgcgttctc gccgacattc acgaatggat cgaatccgac cgggatcggg taggggaggt
     1501 gctgggatga agctttccgt tgtgatcccg gcccgggacg aagagggctc gatcatggag
     1561 accgtcaacg gggcggtcga ggtgatggag cgcgagtcga tcgactacga gattctcgtg
     1621 atcgacgatg gcagcaagga ttccacccga cggctggtcg aggaagcagg cgagagcaac
     1681 ccgaagatcc gttgccggcc gtcgccgtat tccggaggat ttggcctggc ggtgagggcc
     1741 gggctcgaag agttccaggg tgacggagtc gcgatcatga tggccgacgc atccgatgac
     1801 ccggaggacc tcgtgatgta ccaccacatc ctcgacgagg gatgggactg cgccttcggg
     1861 tcgagattca tgccgggtgc cgtcgtcacc gactacccgc ggctgaagct gatcgtcaac
     1921 cgcatggcca atgcattcgt caacgtcctt ttccggctcg gttacaacga cacgaccaat
     1981 gccttcaagg cctatcggcg cgaggtgatc gaaacgatca ggccgcttct ttccaagcac
     2041 ttcaacctga ccgtggagat gccgctcaag gcagtgaccc gcggccacag cttcaaggtc
     2101 gtgcccacct cctgggccaa tcggacctcg ggcgaggcca agctggcgat gagggagatg
     2161 ggtagccggt acctgttcat cgtgctctac atctggctcg aataccacct cagtcgcggc
     2221 gattaccgca aggactgaga gtccgcccgg gccgggcata cggtcactct gccggcaccc
     2281 tgaaagccga tatgccaagg cagatcggaa ttgtcccggg ggatcctgta accgatcggt
     2341 ttgcctgcgg aggccgacca ggcggcggcg ccgcggccgg tcccgaagcg gttgagccgg
     2401 atcagcacgt ccttctcggc ggagatccag gagaggcccg ggtcgatccg gatcttgccg
     2461 ctcgcctcac tgtgcgcggc gagctcccgg cagcgatcac ggatggggat caccctcgtc
     2521 gccggaaccg gctgaaccgg aagcccgagg accagcagct gatccagacg atcccggttc
     2581 tcatccgacg cttcctctgc ctgtgcttcc gagagagccg gtgaaccgta gcggtcgatt
     2641 gccttgaaat acgaggccgc gtcgaccctg gcgctgtcgt cgacaccggc acctacctcg
     2701 aagtcgggac tcaccgtacg tctcgccagg tcgaaggcgg agaagctgat caggttcttc
     2761 tgctcgtatt tcttgtaacg gtcgtggaac gcgaagacca gggtggcggc gttcaagacg
     2821 atcgcgaaga tggcgaccgc agcgatcacc ctgacggtcc gcggtgcggg ccggtacccg
     2881 gcgaaggctc cgcccaggat catcagcagc atgaccacgc tcgggtactg gtatctgctt
     2941 gagacgaact cgcggccggg gatgaaattg agccccgaca ggacccagaa gctgaccccg
     3001 atcgccgccc cgaccaggaa ctcgggcggg accctgccgc g
//
LOCUS       DAOTIP010000176         3029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38367_length_3029_cov_3.427034, whole genome shotgun sequence.
ACCESSION   DAOTIP010000176 DAOTIP010000000
VERSION     DAOTIP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38367_length_3029_cov_3.427034"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="P5138_04310"
     CDS             <1..1375
                     /locus_tag="P5138_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HRV59828.1"
                     /translation="NGEKLTSPPPRSPRRGLKSRPDLKAPRIRVTRTSEQVAPGSYFL
                     GFRRGGLAILNNRGRVTWYRPTSFGFNNFEPQILNGRPVLTYFRTPTPKREGAYVILN
                     RRYRKIAEVTPGNGYKADLHEFRLTGRGTALVLAYRSVRWNLSSVGGPVNGRVVDNVV
                     QEVDIKTGAVLFEWHALGNINLRDSTEPRPGPGRPWDAFHLNSIDADGNSLLVSSRWA
                     NSVFRVGRAGGRIKWVLRGDGGPSDFKVGGRSHFSGQHDVRRLADGNISIFDNAGAPG
                     RRAASVLILRLKGKKNGKRRAIRVARYRNPQGRPSLATGGGEVLPDGNVLAGWGNSNS
                     LTEFSPNGEVLFNARYRSTAYRARKAVWDGKPQGRPAIASVDGAEGTTVYVSWNGAQD
                     IDRWEILSGPDAEDLQVVGDSRWKNLETTIPVSGADSLVQAVALDEDGQELGRSDLVE
                     VGRRSGP"
     gene            1385..2674
                     /locus_tag="P5138_04315"
     CDS             1385..2674
                     /locus_tag="P5138_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59829.1"
                     /translation="MKGSEERSKSAWFGTLGAAMEWYDFTLYVYLAPVISQLFFPSDN
                     SLDSLLATFGVFAAGYLMRPLGAAILGDIGDTRGRKFALGLSISVMALGMLLTGLLPT
                     EASIGALAPILLVLIRMAQGFSLGGEFSGSIVLLAESAKPRHRGFTTNLVQISTGIGF
                     LLSSATVAILHALLDNHQMDTWGWRLPFFIGAAIGVVALVLLITKVPIEDPEEKDKPD
                     DVPLKTLFENERAPLIINFLLNGHQALIYYVVATFIPTFVTSILGGPPQDGLFASALA
                     AIVFMALCPPAGSLSDRYGRRPILAIAAVLMAIAAVPLFALISKVELPAVIGGQLGLM
                     VLVVLFTSPALVIATELFSRETRYSGVAVGYNLGATLFGGTAPLVCTALISITGWNLS
                     PGLLLAVGSILVLIVIHYAPETAPVRTGEGSAPSPGS"
     gene            complement(2658..>3029)
                     /locus_tag="P5138_04320"
     CDS             complement(2658..>3029)
                     /locus_tag="P5138_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59830.1"
                     /translation="VAVVSLVLTGLFGCGDSSGSADEDQIRQVAADFSKAIADGDYQG
                     ACDLFAPESIKFFNTQKAVPDGCAGVMRLSYGQKSDEDIKALGEVESLDVNGDKATAD
                     LGGGESAQYEKIDGNWVMTLD"
BASE COUNT          506 a         1008 c          974 g          541 t
ORIGIN      
        1 caacggcgag aagttgactt caccaccgcc ccgctcaccc cgccggggac tgaaatctcg
       61 gcccgacctg aaggcaccga ggatcagggt cactcggacc tcggagcaag tcgctcccgg
      121 cagctatttc ctcgggttcc ggaggggcgg cctcgcgatt ctcaacaatc gtggccgggt
      181 cacctggtac cggccgacct ccttcgggtt caacaatttc gaaccacaga tcctgaatgg
      241 ccggccggta ctcacctatt tccggacccc gactccgaaa cgtgaaggcg cttacgtgat
      301 cctcaaccgg cggtacagga agatcgccga agtgacgccg ggtaacggct acaaggcgga
      361 cctgcacgaa ttccgcctga ccggacgagg cacggccctg gttctcgcct accggtcagt
      421 ccggtggaac ctctccagcg tcggcggtcc ggtgaacgga cgagttgtcg acaacgtggt
      481 gcaggaagtc gacatcaaga cgggggcggt tctcttcgaa tggcatgcgc tcggcaacat
      541 caatctgcgt gacagcacgg agccgaggcc ggggcccggc cgcccctggg atgccttcca
      601 cctgaactcg atcgacgccg acggcaattc gctgctggtc tcttcacggt gggccaattc
      661 ggtcttccgg gtcgggcggg ccggaggccg gatcaagtgg gtgctacgcg gagacggcgg
      721 accatcggac ttcaaggttg gtggccgcag ccacttcagc ggacaacatg acgtgcgccg
      781 actcgccgac gggaacattt cgatattcga caacgccggc gcgcccgggc ggagggcggc
      841 gtcggtgctg atcctgagac tgaagggcaa gaagaacggc aaacggcggg cgatcagggt
      901 ggcccgttac cgcaacccgc agggccggcc cagcctggcc accggaggcg gcgaggtcct
      961 gcccgacggg aacgtgctgg ccggctgggg gaactcgaat tccctgacag agttcagccc
     1021 gaacggggaa gtgctcttca acgcccgcta ccggtccacc gcctaccggg cccgcaaggc
     1081 ggtctgggat gggaagccac agggacggcc ggcgattgcc agcgtggatg gggccgaggg
     1141 cacgaccgtt tacgtgagct ggaacggtgc gcaggacatt gaccgttggg aaatcctgag
     1201 cggtcccgac gccgaggatc tccaggtggt cggcgactcg aggtggaaga acctggaaac
     1261 gacgatcccc gtgtcggggg ccgactcgct ggtacaggcg gtagccctgg acgaagacgg
     1321 acaggagctg ggaaggtcag acctggtcga ggtcggccgc cgatccggcc cataggctgg
     1381 cggggtgaag ggaagtgaag agcggagcaa gtccgcctgg ttcggcacgc tcggggccgc
     1441 gatggagtgg tacgacttca cgctttacgt ctacctcgcg ccggtcatct cgcagctgtt
     1501 cttcccctcg gacaacagcc tcgactcgct gctggccacc ttcggtgtct tcgccgccgg
     1561 ctacctgatg cgcccgctcg gggcggcgat cctcggcgac atcggcgaca cccgtggccg
     1621 caagttcgcc ctcgggctct cgatcagcgt gatggccctg ggcatgctgc tgaccggttt
     1681 gttgccaacc gaggcaagca tcggagcgct cgccccgata ctcctggtcc tgatccgaat
     1741 ggcgcagggc ttctcgctgg gcggcgagtt cagcggatcg atcgtgctgc tggccgagag
     1801 cgccaaaccc cgccaccggg gcttcaccac caacctggtc cagatctcga ccgggatcgg
     1861 attcctgctc agctcggcca ccgtcgcgat cctccatgcg ctgctcgaca accatcagat
     1921 ggatacctgg gggtggcggc tgcccttctt catcggggcc gcgatcggcg tggtcgcgct
     1981 ggtcctgctg atcaccaagg ttccgatcga agacccggag gagaaggaca agcccgacga
     2041 cgttccgctc aagacgctgt tcgagaacga gcgggccccc ctgatcatca acttccttct
     2101 gaacgggcat caggcgctga tctactacgt cgtcgcgacc ttcattccca ctttcgtcac
     2161 ctcgatcctc ggcggcccac cgcaggacgg gctcttcgcc tcggcactgg cggcgatcgt
     2221 cttcatggcc ctctgcccgc ccgccggttc gctcagtgac cgatacggcc ggcgtccgat
     2281 cctcgccatc gcggccgtcc tgatggcgat cgcagccgtt ccactctttg ccctgatcag
     2341 caaggtcgag ttgccggcgg tgatcggcgg tcagctgggt ctgatggtgc tggtggtgct
     2401 gttcacctcg cccgcgctgg tgatagccac cgaacttttc tcccgggaga ccaggtacag
     2461 cggcgtcgcg gtcggctaca acctcggggc gaccctcttc ggcggtaccg ccccgctcgt
     2521 ctgcaccgcc ctgatctcga tcaccggctg gaacctctcc cccggcctgc ttctggccgt
     2581 cggttcaatt ctggtcctga tcgtcatcca ctacgccccg gaaacggctc cggtccggac
     2641 cggcgagggt tctgccccta gtccagggtc atgacccagt tgccgtcgat cttttcgtat
     2701 tgggccgatt cgcctccgcc gagatcggcg gtggccttgt cgccatttac gtccagtgac
     2761 tcgacctcgc ccagggcctt gatgtcttcg tcgctcttct ggccgtagct cagcctcatc
     2821 acgccggcgc agccgtccgg aaccgccttc tgcgtgttga agaacttgat cgattcgggg
     2881 gcgaacaggt cgcaggcgcc ttggtagtcg ccgtcggcga tcgccttcga aaagtctgcc
     2941 gcgacctgcc ggatctgatc ctcatcggcg ctgccggagg agtcgccaca gccgaacagg
     3001 ccggtcagga caagggaaac aaccgccac
//
LOCUS       DAOTIP010000177         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38399_length_3027_cov_3.921602, whole genome shotgun sequence.
ACCESSION   DAOTIP010000177 DAOTIP010000000
VERSION     DAOTIP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38399_length_3027_cov_3.921602"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="P5138_04325"
     CDS             complement(<1..1612)
                     /locus_tag="P5138_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59831.1"
                     /translation="MLWLAFFGLLVFAVYSSGFAPPARAAEDCTIIPGGTIGDPEPEP
                     IPDRAGSPTDKGPGAHGPNPTVNGLPTRVDLHSGKASQNRNYYFAVKSGRLFVKPNYE
                     RTGKRGNWQHVPAPACLDGDIQSIDADDDELTAINSRNQIFGMDQALSIPSRFNWTSR
                     WGFPVWLGDGYTIPKDTVAWAWTVISPRESRYWVDPAGNRHTVGEGKVSHVLSLRGDH
                     RRITMNDPWLPQDRSYEVCGPRRGTFRISNLAASGSVLFVIGSHGDMYTRLWDFDIAG
                     LDNAFFDYSYADQRGVENPAIQLPGAHWVQQPKIHGKITNRISIFTVGRGTLHRTLRV
                     EGRNTKGVHGFWQKDLYTLNRGSAGWKFVPTPKAKLTGRAIPNPPGDTSSVGAGKAEG
                     YRYISNGDGYRAEIPDFNVSCGRALLKVSFDDGKSLDLNLFLDDMVRVYPRARGLDGT
                     PRYVLGTIEVTPRARATGGDGARNFIRDVLGDRKFTTAQIIATTSGLEIPDLGLQFSA
                     ADSQSSGRVPDQGFDRLARRPIGLLLDRE"
     gene            complement(1652..2647)
                     /locus_tag="P5138_04330"
     CDS             complement(1652..2647)
                     /locus_tag="P5138_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59832.1"
                     /translation="MTPGRLPAVSGLVDRAMDFTVLPGFSRIGYGLRRRMFEWQPPEL
                     SGRSVMVTGASSGLGEAAAIDLARCGAKVHMVCRNEERGERARARVNAEAATAGTDRE
                     PVLHLCDLSLVSSIREFASGFLASETPLDVVINNAGVMPSEREQTEEGFELTFATNVI
                     GPYLLTELLLPRLREGKDPRVIVMSSGGMYSSGFSLGDPQLDRRDYRPTGFYAHTKRA
                     EVMMADAWQRREPPGGVTFCSMHPGWAVTPGMSAALPGFHRVTGPILRDAREGADTAV
                     WLAGATADEAPGGLFYEDRRPRAKERIPGTAGSREDGDRLLELLAGITSSVDVEG"
     gene            complement(2644..>3027)
                     /locus_tag="P5138_04335"
     CDS             complement(2644..>3027)
                     /locus_tag="P5138_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59833.1"
                     /translation="EQGEEDSREHLTLALGEGIEWEMELLEPLEGGEESPVDTDLYRA
                     CEEFVEERLPGAVLMPMVSAGFTDSHWVRQARGTVAYGFAPVFDTPIEVYLESMHGAD
                     ERILIGDLKTMAEFHLYAIRRLLVS"
BASE COUNT          499 a         1008 c          978 g          542 t
ORIGIN      
        1 cttcgcgatc gagcaacagc ccgattggtc gccgggcgag tcggtcgaac ccctggtcgg
       61 gcacccgtcc ggacgactga ctgtcagctg cactgaactg caggcccaga tcgggaatct
      121 cgagccccga agtcgtcgcg atgatctgcg cggtggtgaa ctttcgatcc ccgagtacgt
      181 cacggatgaa gttacgggcc ccgtcgcccc cggttgctcg cgccctggga gtgacctcga
      241 tggttcccag cacgtaccgc ggggtgccgt ccagtccgcg tgccctcgga tacacgcgga
      301 ccatgtcgtc caggaagagg ttcaggtcga ggctcttgcc gtcatcgaag ctgaccttga
      361 gcagcgcccg gccgcaggaa acgttgaaat cggggatctc ggcccggtag ccgtctccgt
      421 tggagatgta ccggtatccc tcggccttgc ccgccccgac tgagctcgtg tcaccgggcg
      481 gattgggaat cgcccttccg gtgagtttcg ctttcggggt cggcacgaac ttccagccgg
      541 ccgacccccg gttgagggtg tagaggtctt tctgccagaa gccgtgtacg cccttggtgt
      601 tccggccttc gactcgcaga gtccggtgga gtgtgccgcg gccgaccgtg aagatgctga
      661 tccggttggt gatcttcccg tgaatcttcg gctgctgtac ccagtgagcg cccggcagct
      721 ggatcgcggg gttctccacc ccacgctggt ctgcgtagga gtagtcgaag aaggcattgt
      781 cgagaccggc gatatcgaag tcccagagcc gggtgtacat gtcaccgtgg ctgccaatga
      841 caaacaggac cgagccggaa gcggccaggt tgctgatccg gaaggttccc cgtcggggcc
      901 cgcagacctc gtagctgcgg tcctgcggca gccaggggtc gttcatggtg atccggcgat
      961 gatcgccgcg caggctgagc acatgcgaga ccttgccctc cccgaccgtg tgacggttgc
     1021 cggccggatc aacccagtac cgcgattcgc gcggcgaaat caccgtccag gcccaggcca
     1081 cggtgtcttt ggggatcgtg tagccgtcgc ccagccagac cgggaaaccc cagcgtgagg
     1141 tccagttgaa tcgggaagga atcgagagcg cctggtccat cccgaagatc tggttccgcg
     1201 aattgatcgc ggtcaactcg tcatcgtcgg cgtcgatcga ctggatgtcg ccatccagac
     1261 aggcgggagc cggaacgtgc tgccagttgc cccgcttgcc ggtgcgttcg tagttcggtt
     1321 tgacgaagag ccgtccgctc ttgaccgcga aatagtagtt gcggttctga ctcgccttcc
     1381 cggaatggag gtcgacccgg gtcgggagtc cgttcacggt cgggttggga ccatgggctc
     1441 cgggcccctt gtcggtgggc gagccggccc ggtccgggat cggttcgggc tcgggatcgc
     1501 cgatcgtgcc gccggggatg atcgtgcagt cttcggctgc cctggccggc ggggcgaaac
     1561 cggacgagta cacggcgaat acgagcagcc cgaaaaacgc aagccagagc aagccacgcg
     1621 ttgcggccgt caacccgccc tgtttcaagc ctcacccctc cacatcgacg ctactggtta
     1681 taccagccag caactcaagc agccggtcgc cgtcctcccg ggagccggcg gtgccgggaa
     1741 tccgctcctt tgcccgcggc cggcggtctt cgtagaagag tccgccgggc gcttcatcgg
     1801 ctgtggcgcc ggccagccaa acggcggtat cggctccttc acgggcgtcc ctgaggatcg
     1861 gtccggtcac ccggtgaaag ccgggcaggg cggcgctcat gcccggggtg accgcccagc
     1921 caggatgcat cgagcagaag gtgacgcctc ccggaggctc ccggcgctgc cacgcgtcgg
     1981 ccatcatcac ctcggcccgt ttggtgtggg cgtagaagcc ggtcggcctg taatcgcgcc
     2041 tgtccagctg ggggtcgccc aggctgaacc cgctcgagta catgccgccc gagctcatca
     2101 caatcacccg tgggtccttt ccctcgcgaa ggcgaggcag gagcaactcg gtcagaaggt
     2161 acgggccgat cacgttggtc gcaaaggtca gctcgaagcc ctcctctgtc tgctctcgtt
     2221 cggaaggcat gaccccggcg ttgttgatca cgacgtcgag cggggtttcg gaagccagga
     2281 acccggatgc gaattcacgg atcgaggaaa cgagggaaag gtcgcagagg tgaagcaccg
     2341 gttcgcggtc ggtgccggcc gtcgccgctt cggcgttgac ccgggccctg gccctttctc
     2401 ccctctcctc gttgcgacag accatgtgga ccttcgcgcc gcaacgggcg agatcgatgg
     2461 ccgcagcctc ccccagcccc gagctggccc cggtgaccat gacgctacgg ccagacagtt
     2521 ccggcggctg ccactcgaac atgcgtcgcc tcagtccgta accgatccgg ctgaatcccg
     2581 gcaggaccgt gaagtccatc gcccggtcaa ccagcccgga aactgccggc aggcgcccgg
     2641 gcgtcaactg accagcaggc ggcggattgc gtacaggtgg aattcggcca tcgttttcag
     2701 atctccgatc aggattcgct cgtcagcgcc atgcattgac tcaaggtaca cctcgatcgg
     2761 cgtatcgaag accggtgcga atccgtaggc gaccgttccc ctggcctgcc tcacccagtg
     2821 ggagtcggtg aagccggccg aaaccatcgg catcagcacg gcacccggca gccgctcctc
     2881 gacgaactcc tcacaggccc ggtacaggtc cgtatcgacc ggggactcct ccccgccttc
     2941 gagcggctcc aggagctcca tctcccattc gattccctcc cccagggcca gggtcaggtg
     3001 ctcccggctg tcttcctctc cctgctc
//
LOCUS       DAOTIP010000178         3025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38433_length_3025_cov_3.381481, whole genome shotgun sequence.
ACCESSION   DAOTIP010000178 DAOTIP010000000
VERSION     DAOTIP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38433_length_3025_cov_3.381481"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="P5138_04340"
     CDS             complement(<1..664)
                     /locus_tag="P5138_04340"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRV59834.1"
                     /translation="MTPAAPVRLSSGAARRIALAAQGFGRERPFPRVTMRQVQATIDR
                     IGLLQIDSVNVLARAHLLPLYSRLGPYDVGLLEKASSKRPRRLVEYWAHEASFVPPET
                     HRLLRWRMDRADREAWRTVRAAADQPELLEDVVAEVERSGPVTAGQVAAAVDPDHIPD
                     KSNWGWNWPPVKSALEYLFWSGRITSAGRTGQFERLYDLPERVIPGHVHEAAAPSQDE
                     AIE"
     gene            694..2121
                     /locus_tag="P5138_04345"
     CDS             694..2121
                     /locus_tag="P5138_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HRV59835.1"
                     /translation="MSVHPHIITVNATSEGEDLMSGDPGAHAYRNVIITAATAGLAGL
                     LFGYDTGVIAGALLQITPDFGLTSLGSGMVVGAVPIGAVLGAWIASYRSDKLGRRRLI
                     LSSGIIFIIGALISALSPGSILLVISRVVIGVAIGVASAVAPVYISEIAPPDIRGRLV
                     TFFQLAVTVGILVAYLVGLAFANTDEGWRWMLGLGAVPALALVIGIVRLPASPRWLLM
                     KGREEDARSALQRVRTEGPEAIELEIKEIEASMQVSEGSWRDLLAPAVKAALMVGIGL
                     AILQQITGINTVIYYAPTIIQETGIDSDFSSILASLGVGIINVLMTVVALRLLDSRGR
                     RELLFVGVTGISVSLFLLGLAFLVGADKTWATVMAIGSLMLFVSSFAISLGPIFWLLN
                     AEIYPLSVRSKAASAGTMTNWFFNFLVSLTFLPLIDLLSQTGAFWFYGAIGLVTLWFC
                     WKFVPETKGRSLEQINDIFERRAGP"
     gene            2240..2782
                     /locus_tag="P5138_04350"
     CDS             2240..2782
                     /locus_tag="P5138_04350"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRV59836.1"
                     /translation="MSQTPPCSIRDFRIEDAPAVHRWFNNKEATRTLMEQRESFELEN
                     AEGWTRAATKQEGEDRKYAIEVPGIEEPVGFTALYGLFRQTAPELGCLIGDEVRGKGV
                     GRWAERLTVTRAFEDFGAHRIYGRIPAFNEAAKKVVAAQGWTYEGNMPGHIRRPDGTM
                     LDCEIWGATPEQFFAAVEKW"
     gene            2832..>3025
                     /locus_tag="P5138_04355"
     CDS             2832..>3025
                     /locus_tag="P5138_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59837.1"
                     /translation="MKFTAELVEDSGRAASGPDFFRSAEFLEAEETTHSLLVFEEGGD
                     GSGQTELVASAPLLVRGIPGS"
BASE COUNT          479 a         1041 c          984 g          521 t
ORIGIN      
        1 cctcgatcgc ttcgtcctga cttggcgccg ccgcctcatg cacatggccc gggatcaccc
       61 tctccggcag gtcatagagt cgctcgaact gtccggtgcg tccggccgaa gtgatccgac
      121 cggaccagaa caggtactcg agggcacttt tcaccggtgg ccagttccag ccccagttgc
      181 tcttgtccgg gatgtggtcg ggatccaccg ccgccgcaac ctgccctgcg gtcaccggcc
      241 ccgagcgttc cacctcggcg accacgtcct cgaggagttc cggctggtcg gcggcagcac
      301 ggactgtgcg ccaggcctcg cggtcggcgc ggtccatccg ccagcgcagc agccgatggg
      361 tctcgggcgg gacgaaggag gcctcatggg cccagtactc gaccagcctc cggggccgct
      421 tcgagctggc cttctccagc aggccgacgt catacggccc gaggcgggag tagagcggca
      481 gcagatgtgc ccgggcgagc acgttcaccg aatcgatctg gagcagtccg atgcggtcga
      541 ttgtggcctg gacctgacgc atagtgaccc gcggaaacgg tcgctcccgg ccgaatccct
      601 gggccgccag ggcgatccgg cgggccgccc cggagctcag cctgaccggc gccgcagggg
      661 tcacccgacc ggtccccgaa agctggctgc atcatgtccg tccacccgca cataattacc
      721 gtcaatgcca cttcggaggg ggaggacctc atgagcggag acccgggcgc gcacgcctac
      781 cggaacgtga tcatcaccgc ggcgacggct ggccttgccg gattgctctt cggttacgac
      841 acgggcgtca tcgccggagc gctcctgcag atcacccccg acttcggcct gaccagcctc
      901 ggatcgggga tggttgttgg cgctgtcccg atcggggcgg tactcggtgc ctggattgcc
      961 tcctaccggt ccgacaagct cggccggcgg cggctgatcc tctcctccgg gatcatcttc
     1021 atcatcgggg ccctgatctc ggcactttcg ccgggatcca tcctcctggt catctcgcgc
     1081 gtggtcatcg gcgtggcgat cggcgtcgca tccgcggtgg ccccggtgta catcagcgaa
     1141 atagctccgc ccgacatccg gggccgcctg gtgaccttct tccagttggc ggtcacggtc
     1201 ggcatcctgg tcgcctatct ggtcggactc gccttcgcca acaccgacga aggctggcgc
     1261 tggatgctcg gactcggcgc cgtacccgcc ctggccctgg tgatcggcat cgtgcggctg
     1321 ccggccagtc cccgctggct gctgatgaag ggccgggagg aagacgcccg gtcggcgctt
     1381 cagcgggtcc gcaccgaagg ccccgaagcg atcgaactgg agatcaagga gatcgaagca
     1441 agcatgcagg tgagcgaggg atcctggcga gacctgctgg caccggccgt caaagccgcc
     1501 ctcatggtcg ggatcggcct ggcgatcctg cagcagatca ccggcatcaa cacggtgatc
     1561 tattacgcgc cgacaatcat tcaggagact ggcatcgact ccgacttctc ctcgatcctc
     1621 gcctcgctcg gggtcggcat catcaacgtg ctgatgacgg tggtcgcgct ccgcctgctc
     1681 gactcgcgcg gccggcgcga gctgctcttc gtcggcgtga ccggcatctc ggtttcactc
     1741 ttcctgctcg gcctcgcctt cctggtcggg gccgacaaga cctgggcgac agtgatggcg
     1801 atcggcagcc tgatgctctt cgtttcttcc ttcgccatct ccctcggacc gatcttctgg
     1861 ctgctgaatg ccgagatcta tccgctctcg gtccgcagca aggctgcctc ggccggaacc
     1921 atgaccaact ggttcttcaa cttcctggtt tcgttgacct tcctgccact gatcgacctg
     1981 ctctcgcaga ccggcgcctt ctggttctac ggggcgatcg gcctggtcac cctctggttc
     2041 tgctggaagt tcgtgcccga gaccaagggc cgcagtctcg aacagatcaa cgacatcttc
     2101 gagcggcggg ccggaccata aggagtcggg cggggtcgga cccgccctcc gcaggacacc
     2161 cgcccctccc accggggaca acgaatggtt gggaggagct ctggctggag gtgcttccaa
     2221 gtcttaatag actccagcca tgtcgcagac tcccccctgt tcgatccggg acttccggat
     2281 cgaggatgcc ccggcggtcc accgctggtt caacaacaag gaagcaaccc gaaccctgat
     2341 ggagcagcgg gagagcttcg agctggaaaa tgccgagggc tggacccgcg cggcaacgaa
     2401 gcaggagggc gaggaccgga agtacgcgat cgaggtgccg gggatcgagg agccggtcgg
     2461 cttcacggct ctctacggac tcttccgcca gaccgcgccg gagctgggct gcctgatcgg
     2521 cgacgaggtc cggggcaaag gagtcggtcg ctgggccgag cggctcaccg tgaccagggc
     2581 cttcgaggac ttcggcgccc accggatcta cggacgcatc cccgccttca acgaggccgc
     2641 caagaaggtg gttgccgccc agggctggac ctacgagggc aacatgcccg ggcacatccg
     2701 gcgacccgac gggaccatgc tcgactgcga gatctggggg gccaccccgg aacagttctt
     2761 cgccgcggtc gagaagtggt gaacgggcgc ccacccgtcc ccggcctccc gggggtcggg
     2821 agaccgtcgg cgtgaagttc accgccgaac ttgtcgagga cagcggccgg gccgcaagcg
     2881 ggcccgactt cttccgttcg gccgagttcc tcgaagccga ggaaaccacc cactccctgc
     2941 tcgtcttcga ggagggcggc gacggtagcg ggcagaccga gctggtcgcc tccgcccccc
     3001 tgctggtcag gggtatcccc ggttc
//
LOCUS       DAOTIP010000179         3025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38437_length_3025_cov_3.103704, whole genome shotgun sequence.
ACCESSION   DAOTIP010000179 DAOTIP010000000
VERSION     DAOTIP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38437_length_3025_cov_3.103704"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="P5138_04360"
     CDS             <1..497
                     /locus_tag="P5138_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HRV59838.1"
                     /translation="FLAGIVVRWEEAAMSAEEHGLRVATIRSGHVLDPRGGLLKELLL
                     PFKMGVGGPIAGGKQYMSWIHRHDEVGILLWALENDAASGPVNATAPNPVTNKEFSKT
                     LGKELGRPSILPLPGFAIDIVKGGGVGHAAREGQRVFPRKATRNGYEFKQPELAGALA
                     NLLS"
     gene            complement(498..1526)
                     /locus_tag="P5138_04365"
     CDS             complement(498..1526)
                     /locus_tag="P5138_04365"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRV59839.1"
                     /translation="MKIGLLTGGGDCPGLNAVIRAVVMRGSREWGHEFVGYLDGWKGP
                     LEDQKIDLGPEDVRGILAQGGTILGSSRTNPSAIDGGEQKVIENVRAAGIDGLVAIGG
                     DDTLGVARKLTEDGLGVVGVPKTIDNDLNATDYTFGFDTAVNIVMESLDRLRTTGASH
                     HRALVVEVMGRHAGWIALHGGMAGGADLVLVPEKEFDLAEICSHVEACFEAGVAPLIV
                     ASEGAVPAEGTEVTSAKGTDAFGHVRLGGIAGWLADRIEEKTGRETRSVVLGHLQRGG
                     TPTAFDRVLATRLGYKAIEAASQGDWGNMTTLRGTSIQLAPLADAVDESKIVPADRLT
                     EAGYGYPA"
     gene            complement(1531..1710)
                     /locus_tag="P5138_04370"
     CDS             complement(1531..1710)
                     /locus_tag="P5138_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59840.1"
                     /translation="MLIPVTFFVAIALAVFSYSQGQGGPVAGLVFFGVLFLGAFIHVT
                     QPLVEKAKALKPSKD"
     gene            complement(1722..2540)
                     /gene="trpA"
                     /locus_tag="P5138_04375"
     CDS             complement(1722..2540)
                     /gene="trpA"
                     /locus_tag="P5138_04375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRV59841.1"
                     /translation="MNGPDRIAEAFANAKEEGRAALMPYMMGGFPDRETSAAIARSYA
                     DNGADLIELGVPFSDPLADGPVIHEAAVKSLEAETRLEDVLEICRTVADRLPVVLMVY
                     TNSILGGQGPAAFAAKAVEAGACGVIVPDLPLGEDETIRSAMAGAGLAVVPLVAPTTL
                     GERRAEICQVATGFVYVVSSVGTTGERSEVPAHLKELVEDVEAKSEVPVAVGFGIGSP
                     EHAATVAQVADGVIIGSKLVRIAAEAAPGQAAEAVGEFMSETAAAVASARSGVR"
     gene            complement(2537..>3025)
                     /locus_tag="P5138_04380"
     CDS             complement(2537..>3025)
                     /locus_tag="P5138_04380"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934329.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRV59842.1"
                     /translation="GGSNAIGTFTAFVPDEKVELIGVEAAGHGLDTDKHGASLTAGNT
                     SVLHGSLSAVLTDEEGQILEAHSVSAGLDYPGTGPEHAHLRDIGRARYVAVSDGDALA
                     AFRRLSRLEGIIPALESSHAIAWLLGEGKVGDGDDNYDVVCLSGRGDKDLAEVLGLEG
                     SG"
BASE COUNT          480 a         1067 c          973 g          505 t
ORIGIN      
        1 atttcctcgc cgggatcgtg gtccgatggg aggaagcagc gatgtccgcc gaggagcatg
       61 gactccgcgt cgcgacgatt cgctccgggc atgtgctcga ccccaggggc ggcctgttga
      121 aggagctgct tctccccttc aagatgggcg tcggaggtcc catcgccggt ggcaagcaat
      181 acatgtcatg gattcaccgg cacgacgagg tcggcatcct gctctgggcc ctcgagaacg
      241 atgcggcgag cgggccggtc aatgcgaccg ccccgaaccc ggtcaccaac aaggaattct
      301 ccaagacgct gggcaaggag ctcgggcgcc cgtcgattct tcccctgccc ggcttcgcga
      361 tcgacatcgt caagggcggc ggggttggtc acgcggcccg cgagggtcag cgggtcttcc
      421 cccgcaaggc gacccggaac ggctacgagt tcaagcagcc ggaactcgcc ggcgctctgg
      481 cgaacctgct ctcctgatca ggccgggtag ccgtatccgg cttcggtgag cctgtctgcc
      541 ggaactatct tggattcgtc gacggcgtcg gcaagcggcg ccagctggat cgaagttccc
      601 ctgagggtcg tcatgttgcc ccagtcgccc tgactggcag cttcgatggc cttgtagccg
      661 agcctggtcg cgaggacccg gtcgaaggcg gtcggcgttc cgccgcgctg caaatggccg
      721 agaaccacag aacgggtttc gcggccggtc ttttcctcga tccggtcggc gagccagccg
      781 gcgattccgc ccaggcgcac gtgcccgaag gcgtcggtgc ctttggccga ggtgacctcg
      841 gttccctccg cgggcacggc cccttccgag gccacgatca gcggtgcgac ccccgcctcg
      901 aagcatgcct ccacgtggga gcagatctcg gccaggtcga attccttctc cggcaccaga
      961 accaggtcgg caccacccgc catgcctccg tgcagggcga tccatcccgc gtggcggccc
     1021 atcacctcga ccaccagggc ccggtggtga ctggcccccg tggtccggag ccggtccagc
     1081 gactccatga cgatgttcac cgcggtgtcg aagccgaagg tgtaatcggt cgcattcagg
     1141 tcgttgtcga tcgtcttcgg caccccgacc acgccgaggc cgtcctcggt cagcttccgg
     1201 gccacaccga gggtgtcgtc cccgccgatc gcgaccagac cgtcgattcc ggcggcccgc
     1261 acgttctcga tcaccttctg ctcgccaccg tcaatcgccg aagggttggt gcgtgatgac
     1321 ccgaggatcg tgccaccctg ggcgaggatg ccccgcacgt cctccggccc gaggtcgatc
     1381 ttctggtcct cgagggggcc cttccagcca tcgaggtaac cgacgaactc atggccccat
     1441 tcccggctgc cccgcatgac tacggcacgg atcacggcgt tgaggccggg acagtcgccc
     1501 ccgccagtca acagtccgat cttcaaggga tcagtccttg ctcggcttga gtgccttggc
     1561 cttctcgacc agcggctggg tgacgtgaat gaacgcgcca aggaacagca caccgaagaa
     1621 gaccaggccg gcgaccgggc cgccctggcc ctgcgagtac gagaagaccg ccagggcgat
     1681 tgcgacgaag aatgtgaccg ggatcagcat cggcgcgaat cctacctgac cccggagcgg
     1741 gccgacgcca cggctgccgc cgtttcggac atgaattccc cgactgcctc cgcggcctgt
     1801 cccggcgcgg cttcggccgc tatccggacc agtttcgagc cgatgatcac cccgtcggca
     1861 acctgcgcca ccgtggccgc atgctcgggg gatccgatcc cgaatcccac cgcgaccggc
     1921 acctcggact tcgcctcgac atcttcgacg agttccttca gatgggcggg gacctcgctt
     1981 cgctccccgg tcgtgccgac gctcgagacc acgtatacga agccggttgc cacctggcag
     2041 atttccgccc gccgttcacc gagggtggtc ggcgcgacca gcggaaccac ggccagtccg
     2101 gccccggcca tcgccgagcg gatggtttcg tcctcaccga gcggaaggtc gggcacgatc
     2161 accccgcagg caccggcctc gaccgccttg gccgcgaagg ccgccggtcc ctgaccgccg
     2221 aggatcgagt ttgtgtagac catcagcacg acgggcaacc ggtcggcgac ggtccggcag
     2281 atctccagca cgtcttccag cctggtttcc gcttccagag acttcaccgc agcttcgtga
     2341 atgaccggcc cgtcggcgag cgggtcggag aaggggaccc cgagttcgat caggtcggct
     2401 ccgttatcgg cataggaacg ggcgatggcc gcggaggttt cccggtcggg gaagccgccc
     2461 atcatgtacg gcatcagtgc cgccctcccc tcctctttcg cgttggcgaa cgcctcggcg
     2521 atccggtcgg gaccgttcac ccggacccct ccagcccgag tacctcggcc aggtccttgt
     2581 cgccgcggcc ggaaagacag accacgtcat agttgtcgtc gccgtctccg accttgcctt
     2641 caccgagcag ccaggcaatc gcatgcgatg actcgagcgc ggggatgatg ccttccagcc
     2701 tgctcagccg gcggaaggcc gcgagcgcgt cgccgtcgct caccgcgacg tagcgggcgc
     2761 ggccgatgtc ccgaaggtgg gcgtgctccg gcccggtgcc gggatagtcg aggccggcag
     2821 acaccgaatg ggcttcgaga atctgccctt cttcgtcggt gagcactgcc gagagcgaac
     2881 cgtggagcac cgaagtgttg cccgcggtca gcgaggcccc gtgcttgtcg gtgtccagcc
     2941 cgtggccggc cgcctcgacc ccgatcagtt ccaccttctc gtccgggacg aaagcggtaa
     3001 aggttccgat cgcgttggag ccgcc
//
LOCUS       DAOTIP010000180         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38613_length_3015_cov_3.745608, whole genome shotgun sequence.
ACCESSION   DAOTIP010000180 DAOTIP010000000
VERSION     DAOTIP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38613_length_3015_cov_3.745608"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="P5138_04385"
     CDS             complement(<1..655)
                     /locus_tag="P5138_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HRV59843.1"
                     /translation="MTLKEMQLLSPARLELYNRQRRAYASAMPPIRQLYAKPQELVEA
                     AIERNEFKPPGARTGVIIDGKSANGKTTMMMDIGRKYEKQQRLIHPAKETEAGGHFTP
                     VIYLNVDSIPTMKSLNRSFLEFYGMDPKRATTNQMTSLLLDLIETCGTTLILIDEVHM
                     LALGRESDRDVNNHLKILANSARATFVYAGVELAKNDFLVEGFAPTEVSRAQISRRFK
                     "
     gene            complement(738..2693)
                     /locus_tag="P5138_04390"
     CDS             complement(738..2693)
                     /locus_tag="P5138_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59844.1"
                     /translation="MVGKVWFDETVAEIADASTDGIVVRLPNGVEHRFIPDGPLPTVD
                     QDPAGTWLDSGTLSKNQTGLASWKLAHIQEAVTGFASGDPGAPREGEPRPQYDPTVHS
                     LSERIRAKAEEIPYDERTLYNWKKKIPEGAGAFIDGRSMKSKTGVDMPEEVKQAVAEV
                     AEEYAESSDLTNDQFWFLVVKHMGDTELPDKSLATLNRYLAQVGVRDGLDRNAKSRRE
                     FVKSPRDDLGHLHSDRPGEVIVIDTTRVDLHALDPVSLEWAQLDLTVAMDLYTRSIVS
                     FRFTPTTKGVDLALLLSDIVSPAPVDERWPTDIPYPYCGVPETVILREFDLPTGTPLK
                     PRPTVKPETILVDRGLNYQSFAFTTGCAKVGAHIQSARPKRPTDKGWVERLFETANQQ
                     LFMGLPGYTGPNVLARGEKPEDKAVFFIWELEQIFGRWIAEIYQERPHDGLRPVECPR
                     YPLTPNQAYEEGIARSGFVKVPSDPNFNLHLLPVIFKHIRNDGISHRSLDFDDPILNQ
                     FRYMPSPHPENKWPFRCDPRDLRKVWFEHPNTGEWHEIYWRFGREIQRPLSEESLSFV
                     KRELRSGKRPPTQKRLRQRLLDFQLDLEDEILVREDRLLRREVMRATSTERQVRGPLP
                     APVLPDLPEFVESSWTTEIAELGLRND"
     gene            complement(2671..>3015)
                     /locus_tag="P5138_04395"
     CDS             complement(2671..>3015)
                     /locus_tag="P5138_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59845.1"
                     /translation="TADFCRQEGIEYRVMSEADPQLLENLRWLCGYRDVPLDHSIDFT
                     RMWTMLEEGPRSFAELIESGEVECLARPVLFGGLWSRHFSTDLLQPFTRETEIRRSDV
                     SSTGRNGGQGLV"
BASE COUNT          552 a          835 c          907 g          721 t
ORIGIN      
        1 gcttgaagcg acggctgatc tgtgctcggc tcacttcggt tggtgcgaac ccttcaacca
       61 ggaagtcgtt cttagccagt tccacgccgg catagacaaa tgttgctctt gccgagttgg
      121 cgagaatctt caggtggttg tttacgtcgc ggtccgattc ccggcccagt gcgagcatgt
      181 gaacttcgtc gatcaggatc agtgttgtgc cgcaggtctc aatcaggtca agcagaagcg
      241 acgtcatctg attcgtggtg gctcgcttgg gatccatccc gtagaactcg aggaagcttc
      301 gattgagact cttcatcgtg gggatcgagt cgacattgag gtagatcacc ggggtgaaat
      361 gaccgcccgc ttccgtctcc tttgctgggt ggatgaggcg ctgttgtttc tcgtactttc
      421 gaccgatgtc catcatcatt gtggtcttgc cgttggcaga ttttccgtcg atgattaccc
      481 cggtccgggc gcccggtggc ttgaattcgt ttcgctcgat cgctgcctcg accagctctt
      541 gtggtttggc gtagagctgg cgaatcggtg gcatcgctga tgcgtacgcc cgtcgttgac
      601 ggttgtagag ctcaagccgg gcaggactca gcagctgcat ctccttgagg gtcaagggct
      661 cgggcggttt gggattcggc cttgtcacga agtcccacca gccctccttg agagtttccc
      721 tcttgcggcc aggtcgctca atcattgcgg agccccagtt ctgcgatctc ggttgtccag
      781 ctggactcga cgaactcagg aaggtcgggg agcacagggg caggcaaagg tcccctgacc
      841 tgacgctcgg tgcttgtcgc tctcatcact tccctcctca acaagcggtc ttcgcgaacc
      901 aggatctcgt cttcaagatc aagctggaaa tcaagcagtc gctgtcgaag ccgcttttgg
      961 gttggaggcc tcttgccgga tcgcagttca cgtttgacga acgaaagtga ctcctcactt
     1021 agaggccgtt ggatctcccg gccgaaacgc cagtagatct catgccattc gcccgtgttg
     1081 ggatgctcaa accacacttt ccgaagatcg cgggggtcgc atctgaacgg ccatttgttc
     1141 tcagggtgag gtgatggcat gtaccggaac tggttgagga tcggatcgtc gaagtcgagg
     1201 ctcctatgcg agatcccgtc gtttcgaatg tgtttgaaga tgacgggaag aaggtggaga
     1261 ttgaagttcg gatcgctcgg gactttcacg aacccggacc tagctatccc ctcttcgtag
     1321 gcctggttgg gtgtcagggg gtatcgggga cactcaactg gtctgaggcc gtcgtgcggc
     1381 cgctcttggt agatctcggc gatccatcta ccgaagatct gttcgagctc ccagataaag
     1441 aagacggctt tgtcctcggg cttttcgcca cgcgccagaa cgttcgggcc ggtgtatccg
     1501 ggcaagccca tgaagagttg ctggttggcc gtctcaaaga gtcgctcaac ccagcctttg
     1561 tccgtgggtc gcttcggtcg tgccgattgg atgtgcgccc cgactttcgc gcatccagtt
     1621 gtgaacgcga aggactggta gttgagtccg cggtccacca aaatggtttc tggtttcacg
     1681 gtgggacgag gctttagcgg cgtacccgtc ggaaggtcga actctctcag gatgaccgtc
     1741 tcgggaactc cacagtacgg gtagggaatg tctgtcggcc atcgctcatc gaccggggcc
     1801 ggcgagacaa tgtccgagag aagcagcgcg aggtcgacgc ctttggtggt cggtgtgaac
     1861 ctgaagctga cgatggatcg ggtgtagaga tccatcgcta ccgtcagatc gagttgcgcc
     1921 cattcaagtg acacgggatc tagcgcgtga agatcaaccc gggtggtgtc gatcacgatc
     1981 acttcgcccg gccgatctga atggagatgc ccgaggtcat cgcgcggcga cttcacgaac
     2041 tcccgtctcg actttgcgtt tcgatctagg ccgtcccgaa caccaacctg tgcgaggtag
     2101 cggttcaaag tcgcaaggga tttgtccggg agttccgtat ctcccatgtg cttgaccacg
     2161 agaaaccaga actggtcgtt tgtcagatca ctgctctctg cgtattcctc ggcgacctcg
     2221 gcgaccgctt gtttgacttc ttccggcatg tcgacaccgg tcttggactt catcgagcga
     2281 ccgtcaataa aggcgccggc gccttcggga atcttcttct tccagttgta gagggttcgt
     2341 tcgtcgtagg ggatttcttc ggctttcgcg cgaattcgtt cggacagtga atggactgtc
     2401 gggtcgtact gcggccgtgg ctcgccttcc ctgggtgctc cagggtcacc cgacgcaaat
     2461 ccggtgacgg cctcttggat gtgggcgagt ttccaggacg cgagacctgt ctggttcttc
     2521 gagagggttc cgctatcgag ccacgtcccg gccgggtcct gatcaaccgt tggtagcggc
     2581 ccgtctggaa tgaatctgtg ctcgactcca ttggggagcc ggacgacgat tccgtcagta
     2641 gaagcgtctg ctatttccgc aactgtttcg tcaaaccaga ccttgcccac cattccgacc
     2701 cgtcgaactg acatcacttc tcctgatttc ggtttcgcgt gtgaacggct gaagaagatc
     2761 ggttgagaag tggcggctcc ataagccacc gaacaggacg ggacgggcga ggcattccac
     2821 ctctccactt tcgatgagct ccgcgaagga gcggggtccc tcctcgagca tggtccacat
     2881 ccgggtgaag tcaatcgagt ggtcaagtgg aacgtcgcga tatccacaga gccaacggag
     2941 gttctccaag agctggggat ccgcttcgct catcaccctg tattcgattc cttcttgtcg
     3001 gcagaagtca gcggt
//
LOCUS       DAOTIP010000181         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_38769_length_3006_cov_3.261606, whole genome shotgun sequence.
ACCESSION   DAOTIP010000181 DAOTIP010000000
VERSION     DAOTIP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38769_length_3006_cov_3.261606"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="P5138_04400"
     CDS             complement(<1..710)
                     /locus_tag="P5138_04400"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRV59846.1"
                     /translation="MSDRLPLDGIRVLDLTRLLPGGFCSQMLVDFGAEVIKVEDTGAG
                     DYIRYAPPLHEVTDPEAGADEGATRSGLYLSLNRGKRSIRLDLKSEEGRKAFYRLVPT
                     ADVVLEGFRPGIAEKLGVDYETLSHIDPGLVYCSISGYGQDGPARDRAGHDINYLGAT
                     GMLAMTGQAGGPPIQPAGQIGDLGGGAMNAAFGILAALIEKGKSGKGQMVDISMTDGA
                     FAWLAMAARCSRKSASGA"
     gene            complement(707..1126)
                     /locus_tag="P5138_04405"
     CDS             complement(707..1126)
                     /locus_tag="P5138_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRV59847.1"
                     /translation="MNDEPHTYTTRLVLEGGEDIRAHRVEMGAQSLAASSAPEFGGDP
                     EKADPEEMTIAAISSCHMLWFIAYARAKRHPVSSYEDQAEIVLDGDRFTSAVLRPKVE
                     FEGERPDDDFIDGLHERAHKACFVANSVNFPVDVEPQ"
     gene            complement(1128..2012)
                     /locus_tag="P5138_04410"
     CDS             complement(1128..2012)
                     /locus_tag="P5138_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRV59848.1"
                     /translation="MSKFESPVIISNSISGVIANRDQCPAIPYTPEEYGAEARRAVDE
                     GASQIHIHARKPDGTPSYEIEDFQAITEAIKAECPDVIINYSTGAIGIPIEKRIAYLE
                     ACRPDVAALNMSSMNYAKYSKRRKDFVFKAVFENSFDTIVEFITAMNELGIKPEHECF
                     DAGHVANLDPLYDMELIKPPSQISLVMGVTGGIRPTPRNVGVMADQVPGGPEGPNNWQ
                     VIGISRDQWKLLAASITLGGNVRAGVEDNLYLPDGTMCRSNGDLIGKARQMVEDSGRK
                     VATIAEAREMLGLKPAGA"
     gene            complement(2009..2329)
                     /locus_tag="P5138_04415"
     CDS             complement(2009..2329)
                     /locus_tag="P5138_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59849.1"
                     /translation="MSEVRLNSSGSSAHSGVIRFDGEVFEMFGFATDGSRRIHIATIQ
                     QLELSFKSGMLATPTISMSGRNGGMSGIIQAIKPDEAEAVDLTALVAEVNEAAARYPN
                     GENQ"
     gene            complement(2351..>3006)
                     /locus_tag="P5138_04420"
     CDS             complement(2351..>3006)
                     /locus_tag="P5138_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRV59850.1"
                     /translation="FDINPMFARSLITCFARMGGMSVGIVANQPKQLGGVLDVDSADK
                     AARFINLCDAFNIPLLFLQDVPGFMVGSKVEQQGIIRHGAKMLYAVSRATVPKITVLV
                     RKAYGAGYYVMCGRAYEPDLIVAWPGAEISVMGAEGAVNIIGRSTIEAAEDPEATREA
                     MLNAVRQQIDPYIAAGNAMIDDIIDPRETRQTVIRGLKMAKDKHVERPWKKHGVMPV"
BASE COUNT          477 a          982 c          972 g          575 t
ORIGIN      
        1 caggcgccgc tggccgactt ccgcgaacag cgcgccgcca tggccagcca ggcgaaggca
       61 ccgtcggtca tcgagatgtc gaccatctgg cccttgccgc tcttgccctt ctcgatcagc
      121 gcggcgagga tgccgaaggc ggcgttcatc gcgccgcctc cgaggtcgcc gatctggccg
      181 gccggctgga tcggcgggcc gccggcctgg ccggtcatgg ccagcatccc ggtcgctccc
      241 aggtagttga tgtcatgccc ggcgcggtcg cgggccggac cgtcctggcc gtagccgctg
      301 atcgagcaat agaccagtcc gggatcgata tggctcaggg tttcgtagtc gaccccgagc
      361 ttctccgcta tccccggccg gaaaccctcg agcacgacgt cggcggtcgg gaccagtcgg
      421 taaaaagcct tgcgaccctc ctcgctcttc aggtcgagcc ggatcgagcg cttgccacgg
      481 ttgagcgaga ggtaaagccc ggaccgggtc gcaccttcat ccgccccagc ctcggggtca
      541 gtgacttcat ggagcggcgg ggcgtagcgg atgtaatcgc cggcgcccgt gtcttcgacc
      601 ttgatcacct cggccccgaa atcgaccagc atctgcgaac agaagccgcc cggcagcagc
      661 cgggtaaggt cgaggacgcg gattccgtcg agtggcaggc ggtcgctcat tgcggctcga
      721 cgtcgaccgg gaagttgacc gagttggcga cgaaacaggc cttgtgtgcc ctttcgtgaa
      781 gtccgtcgat gaaatcgtcg tcgggacgtt ctccctcgaa ttccaccttg gggcggagta
      841 ccgccgaggt gaagcggtca ccgtcgagga cgatctcggc ctggtcctca taggaggaga
      901 ccgggtgccg cttggccctc gcgtaagcga tgaaccagag catgtggcag gaggaaatgg
      961 cggcgatcgt catctcctcc gggtcggcct tctccggatc accgccgaac tcgggagcgg
     1021 aactggcggc cagcgactgg gcccccatct cgacccggtg ggcccggatg tcctctccgc
     1081 cttcgaggac gagtcgcgtg gtgtatgtgt gcggctcgtc gttcatctca ggcacccgcc
     1141 ggcttcagtc cgagcatttc gcgggcctcg gcgatcgtgg caaccttgcg gcccgagtcc
     1201 tcgaccatct gccgggcctt gccgatcaga tcgccgttcg accggcacat ggtcccgtcg
     1261 ggcaggtaca ggttgtcttc gactccggcc cggacgttgc cgcccagggt gatcgaagcg
     1321 gcgagcagct tccactggtc gcgggagatg ccgatcacct gccagttgtt cggtccctcc
     1381 ggaccacccg ggacctggtc agccatcacc ccgacgttgc ggggggtcgg gcggatgccc
     1441 ccggtgaccc ccatcaccag cgagatctgc gagggcggct tgatcagctc catgtcatag
     1501 agcggatcca ggttggcgac gtgaccggcg tcgaagcatt cgtgttccgg cttgatcccc
     1561 agctcgttca tcgcggtgat gaactcgacg atcgtgtcga aggaattctc gaagaccgcc
     1621 ttgaagacga agtccttgcg ccgcttcgag tacttggcgt agttcatcga actcatgttt
     1681 agcgcggcca catcgggccg gcaggcctcg agataggcga tccgcttctc gatcgggatg
     1741 ccgatcgcac cggtcgagta gttgatgatc acgtccgggc actcggcctt gatcgcctcc
     1801 gtgatcgcct ggaaatcctc gatctcatac gacggggtgc cgtccggctt gcgggcgtgg
     1861 atgtggatct ggctggcgcc ctcgtccacg gcgcgccggg cctcggcccc gtactcctcg
     1921 ggtgtgtagg gaatggccgg gcactggtcc cggttcgcga tcaccccgga gatcgaattg
     1981 ctgatgatca ccgggctttc gaacttgctc actggttttc tccgttcggg tatctggctg
     2041 cggcttcatt cacctcggcc accaaagcgg tcaggtcaac tgcttcggcc tcgtcgggct
     2101 tgatcgcctg gatgattccg gacatgccac cgttcctccc cgacatgctg atcgtggggg
     2161 tggcgagcat tccgctcttg aaactcaatt cgagttgctg gatcgtcgct atgtggatcc
     2221 gtcgggaacc gtcggtggcg aacccgaaca tctcgaaaac ctcgccgtcg aaacgaataa
     2281 cccccgaatg ggcggaggag ccgctcgagt tcagacgcac ctcactcatc gctgccccct
     2341 gccggtcggc tcataccggc atcaccccgt gcttcttcca gggccgctcg acgtgcttgt
     2401 ccttggccat cttcaggccc cggatcaccg tctggcgggt ttcccggggg tcgattatgt
     2461 cgtcgatcat cgcgttgcct gccgcgatgt acgggtcgat ctgctggcgg accgcgttca
     2521 gcatcgcctc gcgagtcgcc tccggatcct cggccgcctc gatcgtcgaa cgaccgatga
     2581 tgttgaccgc cccttcggcg cccatcaccg agatctcggc tccgggccag gcgacgatga
     2641 ggtcgggctc gtaggcccgg ccgcacatca cgtagtagcc ggcgccatag gccttgcgga
     2701 ccaggacggt gatcttcggc accgtggccc ggctcaccgc gtagagcatc ttggcgccgt
     2761 gacgaatgat cccctgctgc tcgaccttgg agccgaccat gaagcccggc acgtcctgca
     2821 ggaagagaag cgggatgttg aaggcgtcgc agaggttgat gaagcgggcc gccttgtcgg
     2881 ccgagtcgac gtcgagcacg ccaccgagct gcttcggctg gttggcgacg atgccgaccg
     2941 acatcccgcc catacgggcg aagcaggtga tcagcgagcg ggcgaacatc ggattgatgt
     3001 cgaagt
//
LOCUS       DAOTIP010000182         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39094_length_2989_cov_3.653033, whole genome shotgun sequence.
ACCESSION   DAOTIP010000182 DAOTIP010000000
VERSION     DAOTIP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39094_length_2989_cov_3.653033"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="P5138_04425"
     CDS             complement(<1..999)
                     /locus_tag="P5138_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRV59851.1"
                     /translation="MKKTIVVSADRGETRVAVLEAKTVKSKREVAEVYIERRGGRSLV
                     GNIYLGKVDNVLPGMEAAFVDIGLERNGFLHVDEILLPDGQQAPRRGRGQGRRIDELI
                     KPKQEILVQVIKDPIKSKGARLSMNVTIAGRYLVYAPNGTGVGVSRRLADKERDRLRR
                     MVSRTYDGPGGLIVRTAAHGAKKADFTREIAYLHRLDAVLEKRRKDARAPSLVFQEQD
                     LPVRVLRDVFLNEFDKAIIDDQKQFDRVTSFFQRTAPELVEGVEHYQGKKALFEKWEI
                     EKAINSTLGRRIDLPSGGYLIIDYTEALTVIDVNSGSFTGRGKGGLEETITKVNTEA"
     gene            complement(1042..2226)
                     /locus_tag="P5138_04430"
     CDS             complement(1042..2226)
                     /locus_tag="P5138_04430"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRV59852.1"
                     /translation="MESSRLPRQTHEPRLNLLERAGIAGMDGWLVFSALGLLVCSLFT
                     LYEASGSQIVGAPQEYVQRQALYGLVGIIGMIAVSRIDYSRFRELRVGIYTFLCASIF
                     AVFTFGVAARGSTNQLELPFFTFQPAELGKLLLILALAGFVIDGARKGSDIQRTIRIL
                     ALGLLPTGLVLLQDLGTSLVFAAITFAIMVAAGVRWSHLAVIGVLLAAFVTVVLVALP
                     AAGMPVLKEYQQERLTSFLSPSSDIGDAGYQQNQAKVGIGAGGTTGRGDQATQSRYGF
                     VPERHTDFIFAVVAERYGFVGAALVLSLFALLFWRALRLMTLSKNFYGTLVAAGIAAA
                     IIFQVFVNVGMNLGIMPITGIPLPLMSYGGSAIISTFLMIGVLQSIHIESHAAQRSSW
                     ID"
     gene            complement(2226..>2989)
                     /locus_tag="P5138_04435"
     CDS             complement(2226..>2989)
                     /locus_tag="P5138_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV59853.1"
                     /translation="NAGKQAHGPVDMRRALEVSSDVYFYRLGAEMNGTRQLQNWAGDF
                     GFGKATGIDIPGESEGLVPTPKWLNKAHQDQPQYYDPWVIGQNIQLAIGQGYLQASPL
                     QLAVAYATLGNGGTVYKPQLMLQTEDAAGRVKSEAEPQVAGQVSIDPADRQLIMEGLH
                     DAAQAPEGTSYKIFGGFPIKVAGKTGTAERANEADQAWYAVLAPYPNPKIAVVATVEQ
                     GGFGADTAAPVALQVLQAYFDKQAHAVSGGAGAVE"
BASE COUNT          522 a          936 c          966 g          565 t
ORIGIN      
        1 cgcctcggtg ttgaccttgg tgatggtctc ctcgaggccg cccttgcccc ggcccgtgaa
       61 ggaaccggag ttgacgtcga tcacggtcag tgcctcggtg tagtcgatga tcaggtagcc
      121 gccggagggc aggtcgatcc gccggcccag ggtcgagttg atcgccttct cgatctccca
      181 cttctcgaag agcgccttct tgccctgata gtgctcgacc ccctcgacca gttccggcgc
      241 ggtgcgctgg aagaagctgg tcacccggtc gaactgcttc tggtcgtcga tgatcgcctt
      301 gtcgaactcg ttgaggaaga cgtcacgaag cacccggacc ggcaggtcct gctcctggaa
      361 taccagcgac ggagccctgg cgtccttgcg ccgtttctcc agaaccgcgt cgagacgatg
      421 caggtaggcg atttcacggg tgaagtcggc cttctttgcc ccgtgggcag cggtgcggac
      481 gatcagaccg cccggtccgt cataggtgcg gctgaccatg cggcggagac gatcgcgctc
      541 cttgtcggcg agccgtcggc tcacccccac cccggtgccg ttcggcgcgt agaccaggta
      601 acggccggcg atggtcacgt tcatcgagag gcgggcgccc ttcgacttga tcgggtcctt
      661 gatcacctgg acgaggatct cctgcttcgg cttgatcagc tcgtcgatgc gacggccctg
      721 gccgcggccc cggcgggggg cctgctggcc gtcgggcagc aggatctcgt cgacgtggag
      781 gaagccgttc cgttcgaggc cgatgtcgac gaaggccgct tccattccgg gcaggacatt
      841 gtcgaccttg cccaggtaga tgtttccgac cagggagcgg ccgccccggc gttcgatgta
      901 gacctcggcg acctcacgct ttgacttgac ggtcttggcc tcgagtacgg ccacgcgggt
      961 ctcgccgcgg tcggccgata caacaatcgt ttttttcatg ttctaagtcg ttttccgttc
     1021 agattggtgc ccaatggatt ctcagtcgat ccacgagctc ctctgtgcgg cgtgcgactc
     1081 gatgtgaatg ctctgcagga ccccgatcat caggaaagtc gaaattattg ccgaccctcc
     1141 atagctcatc agcggcaagg gtatgccggt gatcggcatg atgccgaggt tcattcccac
     1201 gtttacgaag acctggaaga tgatcgcggc ggcaattccg gccgcgacca gggttccgta
     1261 gaaattcttc gagagcgtca tcagacgcag cgcccgccag aagagcaggg cgaaaaggga
     1321 taagaccagc gccgcgccga cgaatccgta cctctcggct accacggcga aaatgaagtc
     1381 cgtgtgccgt tcgggaacga atccgtaacg ggactgggtg gcctggtctc ctcgtccggt
     1441 ggttccaccg gcgccgattc cgaccttggc ctgattttgc tggtagcccg cgtcaccgat
     1501 atcggacgac gggctgagga acgaagtgag tcgttcctgc tgatactcct tgagcaccgg
     1561 cattccggcc gccggaaggg cgaccagcac gacggtgaca aaagctgcga ggaggacccc
     1621 gatcacggcc agatgcgacc agcgaacgcc ggccgcgacc atgatcgcga atgtgatcgc
     1681 cgcgaacacc agcgacgtgc ccaggtcctg aagcaggacc aggccggtcg gcaggagtcc
     1741 gagcgcgagt atccggatcg tcctctgtat gtcggagccc ttgcgggccc cgtcgattac
     1801 gaatcctgca agcgcgagga tgagcagaag tttgcccagc tcggccggct ggaaggtgaa
     1861 gaagggaagt tcaagttggt tcgtcgaccc gcgtgcggcc acgccgaatg tgaacaccgc
     1921 gaaaatcgag gcgcacagga acgtgtagat accgacacgt agttcccgga agcgggaata
     1981 gtcaatgcgg gagacggcaa tcattccgat gattccgacc aaaccataaa gagcctgccg
     2041 ctgaacgtat tcctggggtg ccccgacgat ctgtgacccg gaggcctcgt agagggtgaa
     2101 cagcgagcag acgagcaggc ccagagccga aaagaccagc caaccgtcca tcccggcgat
     2161 gcccgccctc tcgagcaggt tgagccgtgg ctcatgggtc tggcgaggaa gccgggagga
     2221 ctccatcact ccaccgctcc cgccccgccg gatacggcgt gggcctgctt gtcgaaatag
     2281 gcctgcagga cctggagggc caccggtgcg gcggtatcgg cgccgaagcc gccctgttcc
     2341 actgtcgcga ctacggcgat cttcgggttc gggtaggggg ccagcaccgc gtaccaggcc
     2401 tggtcggcct cattggctct ttcagcggtg ccggtcttgc cggcgacctt gatcgggaag
     2461 cctccgaaga tcttgtacga ggttccctcc ggagcctggg cggcgtcgtg aaggccttcc
     2521 atgatgagct gccggtccgc cgggtcgatc gatacctgtc cggccacctg cggttctgcc
     2581 tccgacttga cccgaccggc cgcgtcctcg gtctggagca tcagctgcgg cttgtacacg
     2641 gtgccgccgt tgccgagagt ggcgtaggcg accgccagtt gcagcgggct cgcctggaga
     2701 tagccctgac cgatggccag ctggatgttc tgcccgatta cccacgggtc gtagtactgg
     2761 ggctgatcct ggtgggcctt gttgagccat ttcggggtcg ggaccagtcc ttccgactca
     2821 ccgggaatgt cgatgccggt cgccttgccg aaaccaaagt ctccggccca gttctgcagc
     2881 tggcgggtgc cgttcatctc cgcgcccagc cggtagaagt agacgtcgga tgaaacttcc
     2941 agcgcccgcc gcatgtcgac cggtccgtgc gcctgcttgc cggcgttgt
//
LOCUS       DAOTIP010000183         2987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39124_length_2987_cov_3.379945, whole genome shotgun sequence.
ACCESSION   DAOTIP010000183 DAOTIP010000000
VERSION     DAOTIP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39124_length_2987_cov_3.379945"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5138_04440"
     CDS             <1..382
                     /locus_tag="P5138_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HRV59854.1"
                     /translation="NPAGANEVIRTLSLEPAPVDLWIALNDRIADGRDVSWIWDADFE
                     MLADRVGKVTCTGTRASEMALRLKYAGWPLARIEVIDDPEMALDHALKAAGSDLFALP
                     TYTALLDLRNLLAARGDTEKYWEG"
     gene            379..1134
                     /locus_tag="P5138_04445"
     CDS             379..1134
                     /locus_tag="P5138_04445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59855.1"
                     /translation="MILSDENSPEVAIWQLVESCGYSADLPVWTELAEGSRSVLDLGC
                     GIGRVTRHLAAEGREVLGVDIDPLLVDELNRISGPEPVSAITGSVTGLSGLNLGRDRF
                     ETIIAPQQLLHILGGRPARLDLLTGVKDRLEPEGIAAFAISEWVPDVSRPVDVLPDVR
                     EIEDRVYASRPVAVEEQPESLTVVRLRQIVGPDGSFEESEDRITLYRMDRHSLAAELE
                     EAGLVATRSIEVPETDRHIGSVIVVARHDAGVP"
     gene            1151..1885
                     /locus_tag="P5138_04450"
     CDS             1151..1885
                     /locus_tag="P5138_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRV59856.1"
                     /translation="MKLRLLSLYPDQMNIYADRGNILFLQHRCEWRGIDFEVTRCGPG
                     DRFDPEAFDLLYMGGGQDRDQKAVAGDLIETKQQSMRIAKESGAAILAVCGGYQLLGE
                     YYELEGEAIAGLGLVDLRTVREPGPRLIGPVAIKVDLDGRDRVLAGFENHGGRTRLGA
                     ATPLGKVMHGFGNNGEDGFEGVREDNLIGTYLHGPLLPKNAWLADHLIATGLEHHHGE
                     RPDLEPLEDSLEQAAHEAALAAARAG"
     gene            complement(1896..2327)
                     /gene="dut"
                     /locus_tag="P5138_04455"
     CDS             complement(1896..2327)
                     /gene="dut"
                     /locus_tag="P5138_04455"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRV59857.1"
                     /translation="MELKVQLLRDSATIPTRSHDGDAGLDLYAAEAAHIGPGERWQVA
                     TGIAVEIPEGHAGLVLPRSGLAKRHGISLVNAPGLIDAGYRGEVGVLLLNNDPAEVFR
                     IKPGDRIAQLLVIPFAAIEPVETETLSETARGEGGFGSTGA"
     gene            complement(2339..>2987)
                     /locus_tag="P5138_04460"
     CDS             complement(2339..>2987)
                     /locus_tag="P5138_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRV59858.1"
                     /translation="GYTNAGKSTLLNALTGAGIGVADRLFHTLDPTTRAFEYEGRRYL
                     ITDTVGFIKKLPHQVVEAFKATLEETVLADLILHVVDASAGEERMLEEMAAADAVLDE
                     IGAGDKPRLLVLNKIDLLDEEERAEARINHPDAVQVSAMTGEGLEHLRKEMERAFEET
                     MAPVELLVPYTEGSRLHELHEVAGKVDREDGPEGVLVRTRLPLGEMHRFADLVTN"
BASE COUNT          493 a         1008 c          977 g          509 t
ORIGIN      
        1 gaatccggcc ggcgccaacg aagtgatccg gaccctctcg ctggaaccgg cgccggtcga
       61 tctctggatt gccctgaatg accggattgc cgatggccgc gacgtctcct ggatctggga
      121 tgccgatttc gagatgctgg ccgaccgggt cgggaaagtg acctgcacgg gcacgagggc
      181 gtctgaaatg gcgctgcgac tcaagtacgc cgggtggccg cttgcccgca tcgaggtcat
      241 cgatgatccc gagatggccc tcgaccatgc acttaaggcg gccgggagcg atctgttcgc
      301 gctgccgacc tacaccgcgc tgctcgacct tcgaaacctg ctggcggccc gcggggacac
      361 ggagaagtac tgggaaggtt gatcctcagc gacgagaact cgcccgaggt cgcgatctgg
      421 cagctggtcg agtcctgcgg atactcggcc gacctcccgg tctggaccga actggcggaa
      481 ggttcccggt cggtgctgga cctcggctgc gggatcgggc gggtgaccag gcacctcgcg
      541 gcggaagggc gtgaggtact cggggttgac atcgacccgc tgctggtcga tgaactcaac
      601 cggatctccg gaccggagcc cgtctcggcc atcaccggca gcgtcaccgg cctgtccggg
      661 ctgaatcttg gacgggaccg cttcgagacc atcatcgctc cccagcagct gcttcacatc
      721 cttggtggca ggccggcccg cctggacctg ctgacgggtg tcaaggaccg gctcgaaccg
      781 gaagggatcg ccgctttcgc aatctccgaa tgggtccccg acgtttctcg tccggtcgac
      841 gtcctgcccg atgtcaggga gatcgaggac cgggtctacg cgagccggcc ggttgcggtc
      901 gaagagcaac ccgaatcgct gaccgttgtc cggctgcggc agatcgtggg gccggacggt
      961 tcgttcgagg aatccgagga ccgcatcacc ctttaccgga tggaccgcca ctcccttgcc
     1021 gccgagctcg aagaagccgg gctggtagcg acccggtcga tcgaggttcc ggaaaccgac
     1081 cgccacatcg gctcggtgat cgtcgtcgcc cggcatgatg ccggggtccc ctgaaccgta
     1141 gggttcccgt atgaagctcc gccttctcag cctctacccg gaccagatga acatctatgc
     1201 ggaccggggc aacatcctct ttctccagca ccgctgcgag tggcgaggga tcgatttcga
     1261 ggtaacccgc tgcggccccg gcgaccgctt cgatccggaa gccttcgacc tgctctacat
     1321 gggcggtggc caggatcgcg accagaaggc ggtggccggc gacctgatcg agaccaagca
     1381 gcagtccatg cgtatcgcca aggagagcgg ggcggcgatc ctcgcggtct gcggcggcta
     1441 ccagctcctc ggcgagtact acgagctcga aggcgaagcg atcgccggcc tcgggctggt
     1501 tgacctgaga accgtgcgcg agcccggacc ccggctgatt ggccccgtcg ccatcaaggt
     1561 cgatctcgat ggccgggacc gggtactggc ggggttcgag aatcacggcg gccgcacccg
     1621 gctcggcgcc gcgacgcccc tcggcaaggt gatgcacggc ttcggcaaca acggtgagga
     1681 cggcttcgag ggagtccgcg aggacaacct gatcggaacc tacctgcatg ggccgctgct
     1741 gcccaagaac gcctggctgg ccgatcacct catcgcgacc ggccttgagc atcaccacgg
     1801 agagcggccc gacctggaac cgctcgaaga ctcactggaa caggccgctc acgaggccgc
     1861 gctcgccgcg gccagggccg gctgaacagc gccagtcagg cgccggtgga gccgaagccg
     1921 ccctcgcctc gggcggtctc gctcagggtc tccgtctcga ccggctcgat cgcggcgaac
     1981 ggaatgacca gcagctgggc aatccggtcc cccggcttga tccggaaaac ctcggccgga
     2041 tcgttgttca gcagcagcac ccccacttcg ccccgatagc cggcatcgat gaggccgggc
     2101 gcgttgacca gggaaatgcc gtgtcgcttg gccagcccgg aacgcggcag gaccagtccc
     2161 gcatggcctt cggggatttc gaccgcgatg ccggtcgcca cctgccagcg ctccccgggg
     2221 ccgatgtggg ccgcctcggc ggcgtaaagg tcaagccctg cgtccccgtc atgcgaacgg
     2281 gtagggatcg ttgccgagtc tcggagcagc tggaccttga gttccatcgc cgggaactct
     2341 agttggtcac gaggtcagca aaccgatgca tctcgcccag cggcagccgg gtgcgaacca
     2401 gcactccctc gggcccgtcc tcgcgatcga ccttgccggc aacctcatgc agctcgtgca
     2461 gcctcgagcc ttccgtgtag ggcaccagca gctccaccgg tgccatggtc tcttcgaagg
     2521 ctctttccat ctcctttcgc agatgctcaa gcccttcgcc ggtcatggcc gacacctgta
     2581 cggcgtcggg atggttgatc ctggcctcgg cccgttcttc ctcgtcgagc aggtcgatct
     2641 tgttcagcac gagcagcctc ggcttgtccc cggcaccgat ctcgtcgagg acggcgtcgg
     2701 cggcagccat ttcctccagc atcctttcct cgccggccga agcatccacg acatgcagga
     2761 tcaggtcggc gagcacggtc tcctcgagcg tcgccttgaa cgcctcgaca acctgatggg
     2821 gaagcttctt gatgaacccg accgtgtcgg tgatcagata cctgcgcccc tcgtactcga
     2881 aggcccgggt ggtcgggtcc agggtgtgga agagccggtc tgccacgcct atcccggctc
     2941 cggtcaatgc gttcagcagg gtcgacttgc cggcgttggt gtagccc
//
LOCUS       DAOTIP010000184         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39445_length_2967_cov_3.135302, whole genome shotgun sequence.
ACCESSION   DAOTIP010000184 DAOTIP010000000
VERSION     DAOTIP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39445_length_2967_cov_3.135302"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="P5138_04465"
     CDS             complement(<1..706)
                     /locus_tag="P5138_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRV59859.1"
                     /translation="MKKLSPIAYVPRRETLQSASLAPVALYLGAYLLVCFFSSDPLVL
                     VAATAGAALAGYGCGAGRAVGFSLRLGGFLALTMIIVNALVTNRGATVLARLGDWPIL
                     GQVEITAEAIAAGAVIGLRVIGTMVVIGVWSACVDPDRILQAVHGVARRSALTATLIS
                     RLVPLAATDYARIGEAAELRGPAAAPVGRGAMAHAPIQVMEMEHDAHGQNLRRMRELA
                     HDFAPPPDACGTWKALY"
     gene            complement(717..1682)
                     /locus_tag="P5138_04470"
     CDS             complement(717..1682)
                     /locus_tag="P5138_04470"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HRV59860.1"
                     /translation="MNHAAKRRRLGGDFVPAVGVAALLAVALALAGCGLGPGEDTGDA
                     SLIVTRDYGSEVLIDKPDIPVNESSNAMRLLDESAELETRYGGEFVQSVNGISGGRDS
                     GRSSDWFFAVNGIVAERGSAQFTITGGDKLWWDYRDWTDAMEIGAVVGSYPAPFSTGY
                     DDRGWGVEVDCMGDATACVMVRKQLEGDGANLEEKGENLRLMVGTWEQISGTPEGQKL
                     EKGPGVSGVFAKFRPGPTAATYPGSSSWHLVGLDARGETTRSFDPAAGLVAAMRKADE
                     PPVWLITGGTEKGVQLAAAALNPEDLERRFAAVVSDGQVSSLPLP"
     gene            complement(1693..2439)
                     /locus_tag="P5138_04475"
     CDS             complement(1693..2439)
                     /locus_tag="P5138_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59861.1"
                     /translation="MTKRFTSPAVGLLLGLALLLGFAAQPAAAAKKIPVNLRVVTYSG
                     KIMFDRTVNTGTATIKPTSACPTLGGRTGPPRTVTGATGLGLLYQASLKYKALRPLKI
                     SDSDFGFGVCGIGGSMAKGEQWWSVYQNYKSTSTGAEGVKLKRRDSILFFLSKTWAEP
                     NPNLLFLKAPTKVRKGATVKVRVFEYDTAGKRSPVEGAKVKGAGALTDARGYTTLKIK
                     GKTKLVARQAGLVPSNRVYVQVKKNGKHRK"
     regulatory      complement(2487..2670)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2723..>2967
                     /locus_tag="P5138_04480"
     CDS             2723..>2967
                     /locus_tag="P5138_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59862.1"
                     /translation="MDFFSHFIERLPLAVGPSWLDPDSLIRTFGMLGVLAIVFMESGL
                     MVGFFLPGDSLLFTAGLLSANGVLPDIWVLLVTIPIAA"
BASE COUNT          444 a         1007 c          957 g          559 t
ORIGIN      
        1 ggtacaaggc cttccaggtg ccgcaggcgt ccggcggcgg cgcgaagtcg tgggcgagct
       61 cgcgcatgcg ccgcaggttc tggccgtgcg cgtcgtgctc catctccatc acctggatgg
      121 gggcgtgggc catcgcgccg cggccgaccg gagcggctgc cgggccccgt agctcggctg
      181 cctcgccgat ccgcgcgtag tcggtcgcgg caagcggcac cagccgggag atcagggtcg
      241 cggtcagggc cgagcgccgg gccaccccgt ggaccgcctg gagtatccgg tcgggatcga
      301 cgcaggctga ccacactccg atcaccacca tcgtcccgat cacccgcaga ccgatcaccg
      361 caccggcggc gatcgcctcg gcggtgattt cgacctgtcc gaggatcggc cagtcgccga
      421 gccgggcgag gaccgtcgcg ccccggttgg tgaccagggc gttgacgatg atcatcgtca
      481 acgccagaaa gccgccgagc cggagggaaa acccgaccgc cctgccggcc ccgcagccgt
      541 agccggccag ggccgccccc gccgtcgccg cgaccagcac gagggggtcc gatgagaaga
      601 aacagaccag aaggtaggcg cccagataca gggcgaccgg agccagcgag gccgactgga
      661 gggtttcccg gcggggaacg taggcgatcg gggatagctt cttcatgttt ggccgttcag
      721 ggcaggggca gcgagctgac ctgaccgtcg ctcaccaccg ccgcaaatct ccgttcgagg
      781 tcttccgggt tgagtgccgc ggccgcgagc tgcacgcctt tctctgtgcc accggtgatc
      841 agccagacag ggggctcatc ggcctttctc atcgcggcga cgagaccggc ggccgggtcg
      901 aatgaacggg tggtctctcc tcgtgcgtcg agacctacca ggtgccagga gctgctcccc
      961 ggataggtag ccgcggtcgg accgggccgg aacttcgcga ataccccgct cactcccggt
     1021 cctttttcga gtttctgccc ttcgggtgtg ccggagatct gttcccaggt gccgaccata
     1081 agcctcaggt tctcaccctt ttcttcaagg tttgcgccat cgccctccag ctgcttcctg
     1141 accatcacgc aggcggtcgc atcccccatg cagtcgacct caacgcccca tcctcgatcg
     1201 tcatatccgg tcgaaaaagg cgccgggtag gagccgacca cggccccgat ttccatcgca
     1261 tctgtccagt cgcggtagtc ccaccagagc ttgtctcccc cggtaatcgt gaactgggcg
     1321 gagccgcgct cggcgactat cccgttcacg gcgaagaacc agtcggagct acgcccggag
     1381 tcccggccgc cggagatgcc gttcaccgac tggacgaatt cccctccgta gcgggtttcc
     1441 agctcggcgc tctcgtcaag gagccgcatc gcgttgctcg attcgttgac cggaatgtcc
     1501 ggcttgtcga tcaggacttc ggacccgtag tccctggtca cgatcaggct cgcatcgccg
     1561 gtgtcctccc cggggccgag cccgcagccg gccagcgcaa gggcgacggc aaggagtgca
     1621 gccacgccga cggccgggac gaaatcgccc ccgagccgcc ggcgtttggc tgcgtgattc
     1681 atcgggtcgc gtctacttcc ggtgcttccc gttcttcttg acctggacgt acacgcggtt
     1741 cgacggaacg agccccgcct ggcgggcaac cagcttcgtc ttgcccttga tcttcagcgt
     1801 ggtgtagccc ctggcgtcgg tgagcgcgcc tgcgcccttc acctttgcgc cctctaccgg
     1861 gctgcgcttg cccgcggtgt cgtattcgaa cacccgtacc ttcaccgtcg ctcccttgcg
     1921 caccttggtg ggtgccttga ggaagagaag gttcgggttc ggctcggccc aggtcttcga
     1981 gaggaagaag agaatcgagt cgcggcgctt cagcttgacg ccttcggcgc cggtgctcgt
     2041 ggacttgtag ttctggtaga ccgaccacca ctgttcgccc ttggccatcg acccgccgat
     2101 gccgcagact ccgaaaccga agtcgctgtc ggaaatcttc agcggacgca gcgccttgta
     2161 cttgagcgat gcctggtaca gcagtccgag tccggtcgct ccggtcacgg ttcggggcgg
     2221 gccggtgcga ccccccaggg tcgggcaggc cgaggtcggc ttgatagtcg ccgtgcccgt
     2281 gttcacggtc cgatcgaaca tgatctttcc gctgtaggtg accacccgca ggtttaccgg
     2341 gatcttcttc gcggcggcag cgggctgcgc ggcgaagccg agcaacagtg ccagcccgag
     2401 cagcagcccg acggcgggtg aggtgaatcg tttggtcatg cgttcctttt gtccggcggc
     2461 cttacgcgag accgcttttt cggtgcgcca catggcgcaa agtcggcagg tcttcggact
     2521 cggacgtttt cgaggaggca gccttcccag accggtggcc cagtggctat gtgccttcaa
     2581 ggacgtccct accgctgcgc gtcagttccg gatttcgacc ggattccctg accccgcttt
     2641 gggggtgcga ccgacttcgc agacgatgat agagcgtcct gttcggccgt gacctgtcaa
     2701 gatgccggat tccccctccc ccatggactt cttctcgcac ttcatagaga ggctgccgct
     2761 ggcggtcggc ccgagctggc tggacccgga ttccctgatc cggaccttcg gcatgctcgg
     2821 cgtgctggcg atcgtcttca tggagtccgg tctgatggtc ggtttcttcc tgccgggaga
     2881 ctctttgctc ttcacggccg ggctgctttc tgccaacgga gtgctcccgg atatctgggt
     2941 cctgctggtg acgatcccga tcgccgc
//
LOCUS       DAOTIP010000185         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39636_length_2956_cov_3.384006, whole genome shotgun sequence.
ACCESSION   DAOTIP010000185 DAOTIP010000000
VERSION     DAOTIP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39636_length_2956_cov_3.384006"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="P5138_04485"
     CDS             complement(<1..509)
                     /locus_tag="P5138_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59863.1"
                     /translation="MTEIREIGKERYGLALTLILASMVFIMAAPKGRWADVIALTLQS
                     FALFASLRAAQPSINLRVICGVLISLVMVAAWTQASFGASVDSGFVHIATVALVLIAA
                     PVITWGIVRQVRERGSITVHTMMGVLCLYLLMSLAFASGFAAVSQVDGKPFFEQGGGW
                     DSINNYLYF"
     gene            complement(506..1180)
                     /gene="pgl"
                     /locus_tag="P5138_04490"
     CDS             complement(506..1180)
                     /gene="pgl"
                     /locus_tag="P5138_04490"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRV59864.1"
                     /translation="MVSRVVADAEAAAVEAARIVEMVAGAAIADRGRFNFAVSGGKTP
                     WRMLELLADGNLDWTRTSLFQVDERVAPTGSPERNLTHLVLTLPLVCQAAIRPMPVGA
                     DDLATAASGYEYSLPDRFDLIHLGLGPDGHTASLVPDDPILEVADRQVAVTTGEYQGT
                     RRMSLTYKAIDRSRQILFLVTGEGKEDALSRLMAHDGSIPAGRIVNPNITVITDIDLP
                     GADHPS"
     gene            complement(1181..2212)
                     /gene="gnd"
                     /locus_tag="P5138_04495"
     CDS             complement(1181..2212)
                     /gene="gnd"
                     /locus_tag="P5138_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRV59865.1"
                     /translation="MEIGIVGLGRMGANIARRLMRDGHRCVVYDVNPEAAKELSGEGA
                     EPAADLAELASKLTAPRAVWIMVPAGDITRETIRNVAAELESGDAIIDGGNTYYHDDI
                     HFAEELGKKGIDHLDCGTSGGVFGLERGYCLMIGGPEAAYKRLEPIFATLAPGVDEAE
                     RTPGLTGDPSPAENGYLYCGPAGAGHFVKMVHNGIEYGMMAAIAEGLNVIKSANAGLL
                     TRDGDAETAPMENPEYYQYEIDVSQVAEVWRRGSVIGSWLLDLTAASLAESPDLKEFS
                     GRVSDSGEGRWTSIAAIDEGVPTPVLTAALHERFYSRGLGDFGDKVLSAMRKQFGGHD
                     EKPAEDGGK"
     gene            2267..>2956
                     /locus_tag="P5138_04500"
     CDS             2267..>2956
                     /locus_tag="P5138_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRV59866.1"
                     /translation="MSKAKDSEATDALVIFGITGDLAKKMTFRALYRLEMRGKLDCPI
                     IGVGRNQDWHHETMRKRAEEAIRGSVDEFDLATFRRLAARMRFVNGNFDEEALYQSLN
                     GELADYERPLFYLEIPPPLFSEVVHGLANAGLTTNARVILEKPFGHDYDSAQELQADL
                     LSVLREDQILRIDHYLGKEPVMDITYLRFANSTLEPVWNSNFVSYVQMTMSENFGVED
                     RGRFYEGVGAIR"
BASE COUNT          527 a          970 c          941 g          518 t
ORIGIN      
        1 ctgaaataga ggtagttgtt gatcgagtcc caacccccac cctgttcgaa gaagggtttg
       61 ccgtcgacct ggctgaccgc cgcgaatccg ctggcgaagg ccaggctcat cagcaggtac
      121 aggcagagca ctcccatcat cgtgtggacc gtaatcgagc cccgctcacg cacctgcctg
      181 acgatgcccc aggtgatgac cggggctgca atcagaacca gggccacggt cgcgatgtgg
      241 acgaagccgg aatcgacgct ggcaccgaag gaagcctggg tccaggctgc gaccatgacc
      301 aggctgatca ggacgccgca gatgacccgg agattgatgg atggctgggc cgcacgcaat
      361 gatgcgaaga gcgcgaaaga ctggagggtg agcgcgatca cgtctgccca gcggcctttc
      421 ggcgcggcca ttatgaagac catcgacgcg aggatcaggg tcagcgcaag cccgtagcgc
      481 tccttcccga tttcccggat ctcggtcaag aggggtggtc ggctccgggc agatcgatgt
      541 cggtgatgac cgtaatgttc ggattgacga tccggccggc agggatgctc ccgtcatgcg
      601 ccatcaggcg ggaaagagcg tcctccttgc cttctcctgt caccaggaaa aggatctggc
      661 gggaccggtc aatcgccttg taggtcagtg acatgcggcg ggtgccctgg tattcgccgg
      721 tcgtgaccgc cacctggcgg tcggcgacct cgaggatcgg gtcgtccggt accagcgaag
      781 ccgtgtgacc gtccgggccg agaccgaggt ggatcaggtc gaaccggtcc ggcagcgaat
      841 actcgtatcc gctcgccgcg gtggccaggt catccgcccc gaccggcatt gggcgtatcg
      901 cggcctggca gaccaggggc agggtgagca ccaggtgggt caggttgcgc tccggactgc
      961 cggtcggggc gacccgctcg tcgacctgga agagggaggt cctggtccag tcgaggttgc
     1021 cgtcggccag cagctccagc atccgccagg gggtcttgcc gccgctaacc gcaaagttga
     1081 accttccccg gtcggcgatc gccgcgccag caaccatctc gacgattcgc gcagcctcga
     1141 ctgcggcggc ctccgcgtct gcgacgaccc gggaaaccaa ttactttccc ccgtcctccg
     1201 ccggcttctc gtcatggccc ccgaactgct tgcgcatcgc cgacagcacc ttgtctccga
     1261 agtcgcccag accgcgcgag tagaagcgtt catgcagcgc ggcggtcagc accggggtcg
     1321 gcactccctc gtcgatcgcg gcgatcgagg tccagcgtcc ctcgccggaa tcggagaccc
     1381 ggcccgagaa ctccttcaga tcaggtgact cggccagcga ggcggcggtc aggtccagaa
     1441 gccaggaacc gatcacgctg ccccggcgcc acacttcggc gacctgcgac acgtcaatct
     1501 cgtactggta gtactcgggg ttctccatcg gcgccgtctc ggcatccccg tctcgggtga
     1561 gcagacccgc gttggcgctc ttgatcacgt tcagcccttc ggcgatcgcg gccatcattc
     1621 cgtactcgat gccgttgtgg accatcttca cgaaatgccc ggccccggcc ggcccgcaat
     1681 acaggtagcc gttctcggct ggcgaggggt cgccggtcag gcccggtgtc ctctccgcct
     1741 cgtctacgcc cggcgccagg gtcgcgaaga tcggctcgag ccgcttgtag gcggcctccg
     1801 gaccgccgat catcaggcag tagccccgct cgaggccgaa gacacccccg gaggtgccgc
     1861 agtcgaggtg atcgatcccc ttcttgccca gttcctcggc gaaatggatg tcgtcgtggt
     1921 agtaggtgtt gccgccgtcg atgatcgcgt cgcctgattc gagttcggcg gcgacgttcc
     1981 tgatcgtctc gcgggtgatg tcgccggccg gaaccatgat ccagaccgcc cggggcgcgg
     2041 tcagcttcga agcaagctcg gccaggtcgg ctgccggctc ggccccttcg ccggacagct
     2101 ccttcgccgc ctcggggttc acgtcataga ccacgcaacg atgtccatca cgcatcaatc
     2161 tccgggcgat gttcgccccc atacgaccaa gcccgacgat cccgatttcc atgccgccat
     2221 attagaaccc gggtcgggtt catacgggcc gggtaccctg cgtgccatga gcaaagcgaa
     2281 ggattcggaa gcaacggatg ccctggtgat cttcggaatc accggtgacc tggccaagaa
     2341 aatgaccttt cgggcgctct atcgcctcga aatgcgaggc aagctggact gcccgatcat
     2401 cggcgtcggg cgcaaccagg actggcatca cgagacgatg aggaagcggg ccgaggaagc
     2461 gatccgcggt tcggtcgacg aattcgacct ggccaccttc aggcgtctgg ccgcccggat
     2521 gcgcttcgtg aacggaaact tcgacgagga agccctctac cagtcgctga acggcgaact
     2581 ggccgactac gagcgaccgc ttttctatct cgagatcccc ccgcctctct tctccgaggt
     2641 ggttcacggg ctggccaacg ccggattgac caccaatgcg agggtgatcc tcgaaaagcc
     2701 cttcggccac gactacgact cggcccagga actgcaggcg gacctgctgt cggtgcttcg
     2761 cgaggaccag atcctgcgga tcgaccatta cctcggcaag gagccggtga tggacatcac
     2821 ctacctgcgg tttgccaact cgacgctgga accggtctgg aacagcaact tcgtctccta
     2881 tgtccagatg accatgagcg agaacttcgg ggtcgaggac cgtggccgct tctatgaggg
     2941 ggtcggggcg attcgg
//
LOCUS       DAOTIP010000186         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39797_length_2948_cov_3.379191, whole genome shotgun sequence.
ACCESSION   DAOTIP010000186 DAOTIP010000000
VERSION     DAOTIP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39797_length_2948_cov_3.379191"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="P5138_04505"
     CDS             complement(<1..270)
                     /locus_tag="P5138_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59867.1"
                     /translation="MSDDFGNRTPEKLMDAESRSMPTLNREGDVYLLNLGDGENRFNP
                     DRNSIIEELLGEVEGAPAPRALVTVATGKVWSNGLDLEWFQANPER"
     gene            312..935
                     /locus_tag="P5138_04510"
     CDS             312..935
                     /locus_tag="P5138_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV59868.1"
                     /translation="MARPRTHDLDSLLDAAEHLLAERGSSAVTIRALAEATGASSGTL
                     YHAFGSRSDLLGRMWLRAAERFLSEQESAIEGLPGEATDPERAEQATIAVASVPATIF
                     ATSPDTATVLLRYRRDEILGRELSEGLRRELEGLDGRLLAVLTSLAEGLWGRRDRAAI
                     ETIAICVVDLPTAVLITRRERVIDPLAALAAAIRGVLAEIPPDPVRA"
     gene            977..1657
                     /locus_tag="P5138_04515"
     CDS             977..1657
                     /locus_tag="P5138_04515"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HRV59869.1"
                     /translation="MGEAIGQTLPLAVGVALSPLAVIAVILLLSTPRARANAVAFLLG
                     WTAGLMVAGGLLLVIGDSSNMSEAGSPAEWTGYLKIGLGLLLLVVAFRQKNKRLAPGE
                     DPALPAWMTRLDSFGPVGSAGAGALLSGINPKNLVLTAAAAVAISQTGAAGADQVVAL
                     VVYALLASVTVAVPLGIYFFMSDRADEILGRLKNRMGRDNAMVMAVICVVIAAKLIGD
                     GITTLSLF"
     gene            complement(1667..2320)
                     /locus_tag="P5138_04520"
     CDS             complement(1667..2320)
                     /locus_tag="P5138_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRV59870.1"
                     /translation="MYESDFDPRDRGRFEDSHDHSGFGPDAGPRGRRHRHGPGRGGPR
                     GHGGGPRGRGPGRGRRARRGDIRQAILLLLEDEPRNGYGLMQEVEDRSGGSWRPSPGS
                     VYPALSQLEDEGLVEMNESGGRKEFSLTDAGKNWVEEHRDEIGSPWDTEGRGMPKELG
                     SLRKSTQALAMAARQLAHTGTTAQLEEARKIVDEARRKLYGILAQSEEEQDQGQGQG"
     gene            complement(2418..>2948)
                     /locus_tag="P5138_04525"
     CDS             complement(2418..>2948)
                     /locus_tag="P5138_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59871.1"
                     /translation="LVHQRPATVKTSLDGTWERFWRVVIARLLSLLGVTLMFITVIGI
                     PFGIRFLVSWTFVQQEVIFTDRSLRDSFRASSDLVRGHWWHTVRTMVPLLLLLTILGP
                     LLGLFLIFTPLPLLLVNVIGSVVYAVVIPFTTAGTTLLYFDLQARQREGKTVSRRSWA
                     FWRPASFGRRIGQPAT"
BASE COUNT          504 a          977 c          931 g          536 t
ORIGIN      
        1 cctttccgga ttcgcctgga accactccag gtcgaggccg ttcgaccaga ccttgccggt
       61 ggcgaccgtg acaagcgccc gcggagcggg agccccttcg acctcgccca gcaactcctc
      121 gatgatcgag ttccggtccg ggttgaaccg gttctcgcca tccccgaggt tgagcaggta
      181 gacgtcacct tcgcgattca gggtcggcat tgatcgtgat tcagcgtcca tgagtttctc
      241 cggtgtccgg ttgccaaaat cgtcggacat gttttagcac acttgttcta aaacttgtat
      301 acccttcttt catggcccgc cccagaactc atgatctcga ctcgctcctg gatgccgccg
      361 agcacctgct ggcggaaaga ggaagctcgg ccgtgacgat ccgggccctg gccgaggcga
      421 ccggggcttc aagcggcacc ctctaccacg cgttcggctc acgctcggat cttctcgggc
      481 gaatgtggtt gcgtgccgcc gaacgattcc tttccgagca ggagtcggcg atcgaaggac
      541 tccccggcga agccactgat cctgaacggg cagaacaggc gaccatcgcc gtggcgtctg
      601 tacccgcgac gatctttgcc acctcgccgg acacggcaac cgtcctgctg cgataccggc
      661 gtgacgagat tctcggacgc gagctttccg aagggcttcg ccgtgaactg gagggcctcg
      721 acggacgtct gctggccgtg ctgacaagcc tcgccgaggg actttgggga cgccgggacc
      781 gggccgcgat cgaaaccatc gcgatctgcg tggtcgacct gccgacggcc gtcctgatca
      841 cccggcggga gcgggtcatc gacccgctgg ccgcacttgc cgctgcgatc aggggcgttc
      901 tggccgaaat cccgcccgat cctgtccgcg cctgaaatcg gcacggggac cctggccttc
      961 ccgataagct gaccccgtgg gagaggcgat cggacagacc ctgccgcttg ccgtgggcgt
     1021 ggccctcagc ccgctggcgg tcatcgcggt catcctgctt ctctcgaccc ctcgtgcgag
     1081 ggccaacgcg gtcgcctttc ttcttggctg gacagcaggg ctgatggtgg ccggagggtt
     1141 gctgctcgta attggcgaca gcagcaacat gagcgaggca ggcagcccgg cagaatggac
     1201 cggctacctg aagatcggac tcgggctgct gctgttggtg gtcgccttcc ggcaaaagaa
     1261 caagcggcta gcacccggtg aagacccggc attgcctgcc tggatgacca gactcgattc
     1321 attcgggccg gtgggttcag ctggtgcggg agccctgctc tcggggatca accccaagaa
     1381 ccttgtcctg accgcggcgg ccgcagtagc gatatcgcag accggagccg ccggcgctga
     1441 ccaggtcgtc gcattggtcg tctacgcctt gctggccagc gtcacggtcg cagttccgct
     1501 cggcatctat ttcttcatga gcgaccgggc ggacgagatc ctcggccggc tgaagaaccg
     1561 gatgggccgg gacaacgcga tggtcatggc agtgatctgc gtggtgatcg ccgccaagct
     1621 gatcggtgac gggatcacca cgctgtcact gttctagcaa gggccctcag ccctggccct
     1681 ggccctggtc ctgctcttcc tcgctctggg cgagaattcc gtacagcttc cgccgggcct
     1741 cgtcgacgat tttcctggct tcctctagct gcgccgtggt gcccgtgtgg gcgagctgcc
     1801 tggcggccat cgccagggcc tgggtggact tacgcaggga accgagttcc ttcggcatgc
     1861 cccggccttc cgtgtcccag ggggatccga tctcatcccg gtgctcttca acccagttct
     1921 tcccggcgtc ggtcagggaa aactccttgc ggcctccgct ctcgttcatt tcgaccagcc
     1981 cctcgtcctc gagctgcgag agcgccggat ataccgaccc ggggctggga cgccagcttc
     2041 ccccactcct gtcttcgact tcctgcatca ggccgtaacc gttgcggggc tcgtcctcga
     2101 gcagcaggag aatcgcctgg cggatgtctc cccgcctggc ccgccttccc ctgccgggcc
     2161 cgcgtccgcg ggggcctccc ccgtgtccgc ggggaccgcc gcgtccggga ccgtgccggt
     2221 gacgccggcc acggggaccc gcgtcgggac cgaagccgga atggtcgtgg ctgtcttcga
     2281 atcgcccgcg atcacgcggg tcgaaatctg attcgtacat ctggaaaacc ttccttcttg
     2341 aatatctcac gatagataac gatatatcgc aatacctcgg atgtcaagcc cccggcctga
     2401 tccgcggttc cggaacgtca ggtcgcgggc tgcccgatgc gccggccgaa gctggccggc
     2461 cgccagaacg cccaggaccg gcgggacacc gtctttccct cccgttgccg ggcctggagg
     2521 tcgaaataga gcagggtcgt gccggctgtg gtgaagggga tcacgaccgc gtagacaacc
     2581 gatccgatca cgttgaccag aagcaacggc agcggagtga agatcaggaa gagccccagc
     2641 agcggcccga ggatggtcag cagcagaagc aacggcacca tggtccggac cgtatgccac
     2701 cagtgcccgc ggaccaggtc gctactggcc cggaaggaat cgcgcaacga ccggtcggta
     2761 aagatcacct cctgctggac gaaggtccag ctgaccagaa agcggatgcc aaagggaata
     2821 ccgataaccg tgatgaacat gagggtcacg ccaagcagtg aaagcagcct cgcaatcacg
     2881 acccgccaga aacgctccca ggtaccgtcg agggatgtct tcactgtggc cgggcgctga
     2941 tggaccag
//
LOCUS       DAOTIP010000187         2947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39815_length_2947_cov_3.417012, whole genome shotgun sequence.
ACCESSION   DAOTIP010000187 DAOTIP010000000
VERSION     DAOTIP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39815_length_2947_cov_3.417012"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="P5138_04530"
     CDS             <1..509
                     /locus_tag="P5138_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRV59872.1"
                     /translation="YWTERAAAQTTELKEKKHRVEDALQAARAALEEGIVPGGGVALL
                     NAQAALDVDSFEDADERTGAQIIHRSLEEPVRQIAENSGLEGSVVVNKVRELKEGEGL
                     NAATGEYGDMVKAGVLDPTVVTRSALQNAASIAKNILVTEAIIAEPPEEGGAAPAMPD
                     MGGMGGMM"
     gene            718..2862
                     /locus_tag="P5138_04535"
     CDS             718..2862
                     /locus_tag="P5138_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRV59873.1"
                     /translation="MERDSRIELAMAIVAVALFIVILWFAPSLARAAEGPVDSSAGSR
                     IPHEVLVGFRSGTDSETRQQVLDSAGAEDVNAIPGLPRARRVELEDGQTIDEAIDELE
                     DEDDVAWVQPNIIGHISEIPDDPDFNQLWAYRNTGQTVDGTSGTADADIDADQAWDVS
                     TGGGARIAVVDTGVDATSPDLENEVDTSLSRNFAGSIETGNVDSSDWADQNGHGTHVA
                     GTIAAEGDNGVGVAGTSWDTDLVAVRACDFDGYCDSATVASALAYAGSIGSRAVNVSL
                     GFSGSASDASVIRSAINQYPDTLYIVAAGNDDESVEQTPTWPCSLNLDNVICVAATDQ
                     NDKLADFSNWGSTSVDLGAPGVNILSTVPNITAKTDDELTNGLSGWTQGGSGSWNLGS
                     LTNGGSYIKLNATGSTTSATLTTGSLDFSGGQSCSSTYYLSAKLRTGQSLTEQYSTDG
                     GASWQSPGPYGAINSQTGVDDQQFYEMSSWFGAADGEGQVLVRFRYTSTGASSQAPVI
                     DIAYPLVSCIDQQPTAGTYDIYSGTSMATPQVTGAAALLAGYEPDLDAGEVRQALLSS
                     VDDLGSLTGKTVTGGRLNLASTLGAGSDQSSSNSSNSNGSDDGSDGTAGSAAATMRIL
                     SVRRMANGKARVKVRVSKAGKVWIKGTRSVAGRTRRVTGATTVTLTVVSRGRIKRRLQ
                     KHLRSQVYVTVGYRPNGGTSRLRSRWVTLNRR"
BASE COUNT          561 a          993 c          946 g          447 t
ORIGIN      
        1 agtactggac ggagagggcg gcagcgcaga cgacagaact gaaggaaaag aagcatcgcg
       61 tcgaggacgc tctccaggct gcccgtgcgg cgctggaaga gggcatcgtc ccgggcggcg
      121 gtgtcgctct cctcaacgcc caggccgcgc tcgacgtcga ctccttcgaa gatgccgacg
      181 agcgcaccgg tgcgcagatc atccaccgct cgctcgaaga gccggtccgg cagatcgccg
      241 agaactccgg tctcgagggt tcggtcgtgg tcaacaaggt ccgtgagctg aaggaaggcg
      301 aaggcctgaa cgcagccacc ggcgagtacg gcgacatggt caaggccggc gtgctcgacc
      361 cgaccgtcgt cacccgttcg gcacttcaga acgccgcttc gatcgccaag aacatcctgg
      421 tgaccgaggc gatcatcgcc gagccgccgg aggaaggcgg cgcagccccc gccatgcccg
      481 acatgggcgg aatgggcggc atgatgtagg gaggcttctg cctccggcat tcaatgccga
      541 gttatcgagg cttcggcctc acgaaagggc cccgggaaac cggggccctt tccatttgca
      601 gagagtcatc tgcgtgttcg ccggagtaaa ccggcaaccg gggtgcctcc cggctcgcca
      661 gttgattccc ggctcgttct tggggaaatc tcaggtttgc tttctaggtt ctccatcgtg
      721 gagcgcgact cgagaatcga attggccatg gcgattgtgg ctgtggccct gttcatcgtg
      781 attctctggt tcgccccatc cctggcccgg gccgcggaag gcccggttga ctcatcggcc
      841 ggcagccgaa tcccccatga ggtgctggtc ggcttccgtt ccggcacgga ttcagagacc
      901 cggcaacagg tcctggattc ggccggagcg gaagatgtca acgcgatccc cggcctgccc
      961 cgtgcccgca gggtggaact ggaagacggc cagaccatcg acgaagcgat cgatgaactg
     1021 gaagacgaag acgacgtggc ctgggtccag ccgaacatca tcggtcacat ctccgaaatc
     1081 cccgacgacc ccgacttcaa tcagttgtgg gcctaccgca acaccggaca aacggtcgac
     1141 ggcacctccg gcaccgccga cgcggatatc gacgccgacc aagcctggga tgtcagcacc
     1201 gggggcggcg cgcggatcgc cgtcgtcgac acgggcgtcg acgcgaccag ccccgacctc
     1261 gaaaacgaag tcgacaccag cctgagcagg aacttcgccg gctcgatcga gaccggcaac
     1321 gtcgattcat ccgactgggc cgaccagaac gggcacggca cccatgtcgc cggcaccatc
     1381 gccgccgaag gtgacaacgg cgtcggtgtc gcgggaacca gctgggacac cgatctggtc
     1441 gcggtccggg cctgcgactt cgacggatat tgcgactccg ccacggtggc ttccgcgctc
     1501 gcctacgcgg gcagcatcgg ctcgagggcg gtcaacgtca gcctcggctt ttccggctcg
     1561 gcctcagatg ccagtgtgat ccgcagcgcg atcaaccagt acccggacac cctgtacatc
     1621 gtcgccgccg gcaacgacga cgaaagcgtc gagcagaccc cgacctggcc ctgcagcctc
     1681 aacctcgaca acgtgatctg cgttgcggca accgaccaga acgacaagct cgccgacttc
     1741 tcgaactggg ggtcgacgtc ggttgacctc ggggcgccgg gcgtgaacat cctcagcacc
     1801 gtccccaaca tcacggccaa gaccgacgat gaactcacca acgggctctc gggctggacc
     1861 cagggcggct ccggcagctg gaatctcggc tcgctgacca acggcggttc ctacatcaag
     1921 ctcaacgcga ccgggagcac gaccagcgcc accctgacca ccggctcgct cgacttctcc
     1981 ggcggccaga gctgctcttc cacctactac ctgagcgcga agctccgcac cgggcagtct
     2041 ctgaccgagc agtactcgac cgacggcggg gcgagttggc aaagcccggg tccctatggc
     2101 gcgatcaact cacagaccgg tgtcgacgac cagcagttct acgagatgag cagctggttc
     2161 ggggcggccg acggcgaagg ccaggtgctg gtccgcttca ggtacacgag caccggcgcc
     2221 tccagccagg ctccggtgat cgacatcgcc tatccgctgg tcagctgcat tgaccagcag
     2281 ccgacggccg gcacctacga catctacagc ggaacctcga tggccacccc gcaggtgacc
     2341 ggtgccgcgg cgcttctggc cggctacgag ccggatctcg atgccggcga ggtccgacag
     2401 gctctgctgt cttcagttga cgatctggga tcactgaccg gaaagaccgt gaccggcggc
     2461 cggctcaacc tggccagcac tctgggcgcg gggagtgacc agagttccag taactcgagc
     2521 aactcgaacg gctcagacga cggaagcgac ggaactgccg gttccgctgc ggcaaccatg
     2581 cggattctct cggtcaggcg gatggcgaat ggaaaggccc gggtgaaggt ccgggtttca
     2641 aaggccggga aggtctggat caagggcacc cggtcggtcg ccggaaggac ccgcagggtg
     2701 accggggcca cgaccgtgac cctgaccgtc gtctcgcgcg gccgcatcaa gcgacggttg
     2761 cagaagcacc tgcgcagcca ggtctacgtg acggtcggct atcgcccgaa cggcggaacg
     2821 agccggctcc gttcacgctg ggtcaccctg aaccgccgct agtactcgcc gagcacctcg
     2881 gccatcggtc tgggctcgac cccgagcgac tcgaggcggc gtcgcagcac atccgcgttc
     2941 aatccga
//
LOCUS       DAOTIP010000188         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_39937_length_2940_cov_3.442634, whole genome shotgun sequence.
ACCESSION   DAOTIP010000188 DAOTIP010000000
VERSION     DAOTIP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39937_length_2940_cov_3.442634"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="P5138_04540"
     CDS             complement(<1..741)
                     /locus_tag="P5138_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59874.1"
                     /translation="MSFYAAPTPSGAADCSTPSDACSIIDAVTAANGASVSDQVDLLL
                     DKGVYQLATANPTALVVTFAGPGLTIEGRNGTPTLNGTNTQRIMSIDAASTVAIDGIE
                     FEHGQSTGPGGAIQNGGTLSIENSNFASNTAANGGAIASNAGSTLMVSDTSFLQNATT
                     GIGGGAVISYSTTTIERSAIFNSTAPINGGGLNVQPGGSLTLANSTVANNVSGSLGGG
                     ISNLGDLKVYGSTVINNRGSDGSAIATGS"
     gene            944..1621
                     /locus_tag="P5138_04545"
     CDS             944..1621
                     /locus_tag="P5138_04545"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRV59875.1"
                     /translation="MPAPPLREVIDPGWAEALEPVAGQISDMGDFLRGEAAAGRGYLP
                     AGENILRAFTLPLDRVRVLIVGQDPYPTPGHAVGLCFSVAPDVRPLPPSLVNIFREYS
                     DDLGYPSPSNGDLSPWFDRGVLLLNRSLSVQPGRPNAHQGKGWEAVTEQAITALAARG
                     GPMVAILWGRNARNLAPLLGQVPRIESAHPSPMAAHNGFFGSRPFSRANQLLVEQGAE
                     PIDWRLP"
     gene            1638..2327
                     /gene="nucS"
                     /locus_tag="P5138_04550"
     CDS             1638..2327
                     /gene="nucS"
                     /locus_tag="P5138_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HRV59876.1"
                     /translation="MRLIVARCEARYTGRLETFLPEALRLVMIKSDGSVMIHADTGGY
                     KPQNWMTPPTTIEELAAEGEEPARIVVTKHAGATEDRVEIKIAEVLSDVTHDMGEAAA
                     LERDGVEAHLQELLAEQPHWCGEGLRLVKREWFTDIGPVDLMCKDPDERWVAVEIKRV
                     GTIEAVEQITRYLERIHNEPTMADCRGVLAAQSIKPQARTLAEGRDIACVEVDLAVLR
                     GEREPDLTLFG"
     gene            complement(2345..>2940)
                     /locus_tag="P5138_04555"
     CDS             complement(2345..>2940)
                     /locus_tag="P5138_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV59877.1"
                     /translation="TRTKNRTIALAPWLIARHSSGLRPPYILPSGGSSPLGSLGYVET
                     AFEIAAQVEAGDLPAPGIVVVPTGSGGTAAGLALGFGLAGLPTRVIGVIVTEHLRLDD
                     RAIARLAGKSAALLRKRGASFREPELNLEMTIDWMGETYGAPTPESERAIELTRAEGL
                     ELDPVYTAKAMAALLDKSRAGDFDARPVLFLNTNGPR"
BASE COUNT          497 a          964 c          974 g          505 t
ORIGIN      
        1 gcttccggtc gcaatcgccg atccgtcaga acccctgttg ttgatgaccg tcgagccgta
       61 gaccttgagg tccccgaggt tggagatgcc accgccgaga ctgcccgaga cgttgttcgc
      121 caccgtcgag ttggcgaggg tcagactgcc tccgggctga acattcagtc ctccaccgtt
      181 gattggcgcg gtgctgttga agattgctga acgctcgatt gtcgtcgtgc tgtaggaaat
      241 gaccgcccca ccgccgatgc cggtcgtggc gttctgcaag aaactcgtat cggaaaccat
      301 gagcgtcgac cccgcgttgc tcgcaatggc gcccccgttc gcagcggtat tggaagcgaa
      361 attcgagttt tcgatcgaga gcgttccgcc attctggatc gcccctcccg ggccggtgct
      421 ctggccatgt tcgaactcga tcccgtcgat ggcgacggtc gatgccgcat cgatcgacat
      481 gatccgctgc gtgttggtgc cgttcagggt gggggtgccg ttgcggcctt cgatagtgag
      541 gcccgggccg gcgaaggtaa ccaccagagc cgtagggttg gcggtcgcaa gctggtagac
      601 gcccttgtcg agcagaaggt ccacctgatc ggagaccgac gccccgttgg cggccgtgac
      661 ggcgtcaatg atcgaacagg catccgaggg tgtggagcag tcggccgccc ccgaaggggt
      721 cggtgcggca tagaagctca cagtcgctgc tcttgctcct gacgcccaca ggagaaaggc
      781 aaacccgaga gccgccgcta ccccgatcaa ccgcctggtc cgaccttcac ttcgcgccaa
      841 aaccatgcct gacatcgaat cccccgatcc cggttcacct ttgttccaac ctagcagcgt
      901 caccgcggac aattcaaccg cctctccgcc tatgctcggt cggatgccgg cgcctcccct
      961 gagagaagtg atcgaccccg gttgggccga ggcccttgag ccggtggccg ggcagatttc
     1021 ggatatgggg gactttctgc gcggtgaagc tgccgccggg cgcgggtacc tgccggccgg
     1081 ggaaaacatt ctgcgggcct tcaccttgcc gctcgatcgg gtgcgggtat tgatcgtcgg
     1141 ccaggacccc tacccgaccc ccggccacgc ggtgggcctg tgtttctcag tcgcgccgga
     1201 cgtgagacct ctgccgccga gcctggtcaa catcttccgg gaatacagcg acgacctcgg
     1261 ctacccgtcg ccttccaacg gtgatctctc gccctggttc gaccgcggcg tgctgctcct
     1321 gaaccgttcg ctctcggtcc agcccggtcg ccccaacgcc catcagggca agggctggga
     1381 ggcggtcacc gaacaggcga tcacggcatt ggcagcacgt ggcgggccga tggtcgcgat
     1441 cctctggggc cgtaacgccc ggaacctcgc gcccctgctc ggtcaggtgc cgcgaatcga
     1501 atctgcccat ccgagcccga tggccgccca caacggcttc ttcggctcgc gtcccttcag
     1561 ccgcgccaac cagctgctgg tcgagcaggg tgccgagccg atcgactggc gcctgccctg
     1621 aggctaggct tctcgcgatg cgtctgatcg ttgcccgctg cgaggcccgt tacaccgggc
     1681 ggctggagac tttcctgccg gaggcactcc gtctggtcat gatcaagtcg gacggctcgg
     1741 tgatgatcca cgccgacacc ggcggctaca agccacagaa ctggatgacc ccgccgacca
     1801 cgatcgagga gctcgcagcc gaaggtgagg aaccggccag gatcgtcgtg accaagcacg
     1861 cgggggcgac cgaggaccgg gtcgagatca agatcgccga agtgctctcc gacgtcaccc
     1921 atgacatggg cgaagcggca gcgctggaac gggatggggt ggaggcgcac cttcaggagt
     1981 tgctggccga acagccgcat tggtgcggcg aaggcctcag gctggtcaag cgggagtggt
     2041 tcacggacat cggcccggtg gacctgatgt gcaaggaccc ggatgagcga tgggtggcgg
     2101 tcgagatcaa gcgggtcggg acgatcgagg cggtcgagca gatcacccgc tacctggagc
     2161 ggatccacaa cgagccgacc atggccgact gccgcggcgt tctcgccgcc cagtcgatca
     2221 aaccgcaggc cagaaccctg gccgaaggcc gcgacatagc ctgcgtggaa gtagatctcg
     2281 ccgtcctccg gggagaacga gaaccggacc tcaccctctt cgggtaaggg ccgggcgggt
     2341 cccgctagcg aggcccgttg gtattcagga agagaaccgg gcgagcgtcg aagtcgccgg
     2401 ctcttgattt gtcgagaagg gcggccatcg ccttcgccgt gtagaccggg tcgagttcga
     2461 ggccctcggc tcgggtcagt tcgatcgccc gctccgactc gggagtaggg gcgccgtatg
     2521 tttcgcccat ccagtcgatc gtcatctcca gattgagctc gggctccctg aacgacgccc
     2581 cgcgttttct cagcagcgcc gcactcttgc cggccagcct ggcgatcgcc cggtcatcga
     2641 ggcgcaggtg ctcggtgacg atgaccccga tcacccgggt cggaaggccg gccagaccga
     2701 atccgagtgc gaggccggcg gccgtgcctc cggaaccggt cgggaccacc acgatgccgg
     2761 gcgccggcag gtctcccgcc tcgacctggg cggcgatctc gaatgccgtc tccacgtagc
     2821 caagcgagcc gagcggcgac gatccgccgg aaggaaggat gtacggtggc ctgagcccgc
     2881 tggaatgccg tgcgatcagc cagggagcca gcgcgatcgt gcggtttttg gtccgcgtga
//
LOCUS       DAOTIP010000189         2935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40038_length_2935_cov_3.307639, whole genome shotgun sequence.
ACCESSION   DAOTIP010000189 DAOTIP010000000
VERSION     DAOTIP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40038_length_2935_cov_3.307639"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2189
                     /gene="lon"
                     /locus_tag="P5138_04560"
     CDS             <1..2189
                     /gene="lon"
                     /locus_tag="P5138_04560"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRV59878.1"
                     /translation="RDPEEDTPSPEQLHDVGVVGVVTRMLKVPDGTIRILVQGSQRVR
                     LGDYVTETPYLVARITEMPDIVEETPELEALTRNVQKTFSDIIEQIPYLPEELQLAVT
                     NVEEPSALSHLIAGALRISTEEKQLLLEEVDVAKRLRMLSEILARELEVIQLGSQIQS
                     EVQSSIDKGQREYFLREQLKAIQTELGEEDEQTAEINELRERIEAAKLPEHAHQAAER
                     ELSRLEKLPPAAAEHGVIRTYLEWLVDLPWSKESEDNLDIDHAREVLDADHYDMEKIK
                     DRILEYLAVRKLNPDSPGPILCFVGPPGVGKTSLGQSIARALGREFERISVGGVRDEA
                     EIRGHRRTYIGAMPGTIVRALRDAGSRNPVFMIDEIDKMGADFRGDPSSAMLEVLDPA
                     QNDAFRDHYLDMPFDLSEVMFIATANVLDTIPGPLRDRMEVIQLAGYTVDEKRHIARR
                     YLVPRQIRANGLKPSQISFTDPALTAIIEEYTREAGVRNLEREIGTVCRKVARKVAEG
                     SGKTKKIKVSGKKVREMLGRRRVFSETRRRTKVPGVSTGLAWTPTGGDVLFIEATAMP
                     GNGKLVITGQLGEVMRESAQAALSYVRANARDLAPGLEEKWFATHDLHIHVPAGAVPK
                     DGPSAGVAITVALVSLITGRPVRNDVAMTGEVTLTGQVLPIGGVKEKSLAAQRAGIKI
                     VILPGRNEGDVEEIPEYERADVEFFYADKVGDALAVALEGDLKGRN"
     gene            2262..2720
                     /locus_tag="P5138_04565"
     CDS             2262..2720
                     /locus_tag="P5138_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59879.1"
                     /translation="MGTENISGKAAKAYSSARENQHVRRLIEDEELRASLIAAAIAGR
                     KAFQRVQTNRSSAVESVTQDKRVKKELQSAAESLRDAADRLKSPPKKKRHPIRKLLLV
                     GVVTGGLVLVFSESARKSLLDMIFGAEEEFVYTSTTSTETAQTNGTPVAD"
BASE COUNT          569 a          960 c          945 g          461 t
ORIGIN      
        1 cccgtgatcc ggaagaagac acgccgagcc ccgaacagct ccatgacgtc ggagtggtcg
       61 gagtggtcac ccggatgctc aaggttccgg acgggaccat ccgcatcctc gttcagggga
      121 gccagcgcgt ccggctgggc gactacgtca cggagacgcc ttacctggtc gcccggatca
      181 ccgagatgcc ggacatcgtc gaggagaccc ccgagctcga agcgctgacc cggaacgtac
      241 agaagacctt ctccgacatc atcgaacaga ttccgtacct gccggaggaa ctccagcttg
      301 cggtgaccaa cgtcgaggaa ccttcggcac tttcccatct gatcgccggg gcgctccgca
      361 tctccaccga ggagaagcag cttctgctcg aagaggtcga tgtcgcgaag cgtctccgca
      421 tgctttccga gatccttgcc cgtgagctcg aggtgatcca gctcggctcc cagatccagt
      481 cggaagtcca gtcctcgatc gacaagggcc agcgcgagta cttcctgcgg gaacagctca
      541 aggcgatcca gaccgagctc ggcgaggaag acgagcagac cgcggagatc aacgaactgc
      601 gcgagcggat cgaagcggcg aagctgcccg agcatgccca tcaggcagcc gagcgggaac
      661 tgtcccgctt ggagaagctg ccgccggctg cggccgagca cggggtgatc cgcacctatc
      721 tcgaatggct ggtcgacctg ccctggtcga aggagagtga ggacaacctc gacatcgacc
      781 atgcccgcga ggttcttgac gccgaccact acgacatgga gaagatcaag gaccggatcc
      841 tcgagtacct ggcggtgagg aagctgaacc ccgactcgcc cggtccgatc ctctgcttcg
      901 tcgggcctcc cggcgtgggc aagaccagcc tcggacagtc gatcgcccgg gcgctcggcc
      961 gggagttcga acggatctcg gtcggcgggg tgagggacga agccgagatc cgcggccacc
     1021 gccgcaccta catcggggcg atgcccggca ccatcgtccg ggcgctcagg gacgccggct
     1081 cccggaatcc cgtcttcatg atcgacgaga tcgacaagat gggagcggac ttccggggcg
     1141 acccctcgtc ggcaatgctc gaagtgctcg atccggccca gaacgacgcc ttccgcgatc
     1201 actacctcga catgcccttc gacttgtccg aggtcatgtt catcgccacc gcgaacgtgc
     1261 tcgacacgat tcccggcccg ctccgcgacc ggatggaagt gattcaactg gccggctaca
     1321 cggtcgacga gaagcgacac atcgcccgtc gctacctggt gccccgccag atccgggcca
     1381 atggcctgaa gccgtcgcag atttccttca ccgatccggc gctgaccgcg atcatcgagg
     1441 agtacacccg cgaggccggg gtgcggaacc tcgaacggga gatcggcacc gtttgccgca
     1501 aggtcgcccg gaaggttgcc gagggaagcg gcaagaccaa gaagatcaag gtttcaggca
     1561 agaaggtgcg ggagatgctc ggccgccgcc gcgtgttctc ggagacccgg cggaggacca
     1621 aggtgccggg ggtttcgacc ggtctcgcct ggacaccgac cggcggtgac gtccttttca
     1681 tcgaagcgac cgcgatgccc ggcaacggca agctcgtgat caccggccag ctcggcgagg
     1741 tgatgcgtga gtccgcccag gctgcgctct cctatgtccg ggccaacgcc agggacctgg
     1801 caccgggact cgaggagaaa tggttcgcca cccacgacct tcacatccat gtccccgccg
     1861 gcgccgtgcc caaggacggg ccctcggccg gcgttgcgat caccgtcgcc ctcgtctccc
     1921 tgatcaccgg ccgccccgtc cgcaatgacg tggcgatgac cggtgaggtg accctgaccg
     1981 ggcaggtgct gccgatcggc ggggtgaagg agaaatcgct cgccgcccag cgggccggga
     2041 tcaagatcgt gattctgccc ggacgcaacg aaggagacgt cgaggagatc cccgaatacg
     2101 agcgggccga cgtcgagttc ttctacgccg acaaggtcgg tgacgccctt gcggtggccc
     2161 tcgaaggcga cctgaagggc cggaattgag ccggttcgtt tttctttgaa ccggggtatc
     2221 ggtattctcg aaagaccact atccgatcaa gcgaggaaca catgggaaca gagaacatct
     2281 cgggcaaggc cgccaaggcg tactcgtcgg cgcgtgagaa ccagcatgtc cgtcgcctga
     2341 tcgaggacga ggaactccgg gcgagcctga ttgcagccgc gatcgcgggg cgcaaggcct
     2401 tccagcgtgt ccagacgaac cggtcttcgg ccgtcgaatc ggtgacccag gacaagcggg
     2461 tcaagaagga gcttcagagc gccgccgaat cgcttcgtga cgctgcggac cgcctcaagt
     2521 cgccgccgaa gaagaagcgc catccgatcc gcaagctgct tctggtcgga gttgtcaccg
     2581 gtggcctcgt gctcgttttc agcgagtcgg cccgcaagag cctgctcgac atgatcttcg
     2641 gcgccgaaga ggagttcgtc tacacctcga cgacctcgac cgaaaccgca cagaccaacg
     2701 gaaccccggt agcggactag ctccgggcgg caccagaggc ccgggcaacg cgcccggggc
     2761 gcacctgcgg gcgccgtggc ttcgtcaacg ccgggaaacg gcagctcagg tacctgagta
     2821 cgatcgcggc catttaccgg cttatttctc gccagccacc cgcccaaggt cgggtgcggt
     2881 agcgcacggt gtaacaagtt ttgtgcgttg tctgcgggaa atgtgtttcg cctcc
//
LOCUS       DAOTIP010000190         2934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40053_length_2934_cov_3.697812, whole genome shotgun sequence.
ACCESSION   DAOTIP010000190 DAOTIP010000000
VERSION     DAOTIP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40053_length_2934_cov_3.697812"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..328)
                     /locus_tag="P5138_04570"
     CDS             complement(59..328)
                     /locus_tag="P5138_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59880.1"
                     /translation="MEDTTVSLDEARLRRLIEVGRSLVVESDPEAVITDALEAARELT
                     GARYAALGVLDTRRVELEQFLTRGVEEKLSEKIGHPPHGEACSAC"
     gene            459..1076
                     /locus_tag="P5138_04575"
     CDS             459..1076
                     /locus_tag="P5138_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV59881.1"
                     /translation="MLVDDHAVVRSALKVLLESEPDLEVVAEAGTADDAIRYVGAHKP
                     DVLVLDLNMPGRPSLEAVPDIQAASPDTKIVILTMRNEPAFARQALRAGVTGYVLKEA
                     ADAELVLAVRRAADGDTYLQPSLYQHLADKDKAEGRTGRTLSDREVEVMRLIALGHTN
                     AEIADQLFLSVRTVESHRASIQKKLDISSRAELVRYALDQGLISA"
     gene            1125..1889
                     /locus_tag="P5138_04580"
     CDS             1125..1889
                     /locus_tag="P5138_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRV59882.1"
                     /translation="MLILLPPSEGKASPSGKTRLDLGSLAFADQLGGKRAELIAALEK
                     LGKAPQKKAIEVLGISKGQAGEIARDAELATAPAAPASELYTGVLYDRLDFGTLTAAA
                     GRRAKGNLLIASALWGFLAPADRIPYYRFSMKAKLPKIGGLPAYWRDALTAAMESAGH
                     DEEGGLVLDMRSGAYSAAWKPKRARLVTVRGFTETGGKRKAISHMAKAVRGEVARTAL
                     SARSMPKDLETLAGLLEKEGKTIELTDKTLDVIEKA"
     gene            1951..>2934
                     /locus_tag="P5138_04585"
     CDS             1951..>2934
                     /locus_tag="P5138_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRV59883.1"
                     /translation="MASILRKSPAGTAVTVVLLLAGLAGLVLLSAAGDNSGDSNATAG
                     SAAVGPFVKSETASSPFPPLTRNLSAGLGINQLAGQRIVAGFEGKSVPPVIRRRIRAG
                     LIGGVILFADNLGSRSQIQRLTRSLQRIGRPAGLRRYPLMIMVDQEGGLVKRLSGAPN
                     ASAEQMGARGAAYSRRQGLLTGRNLKNAGFNIDLAPVLDVARPGGVIADTDRGFGSTV
                     KKVNATAIPFAQALQRTGVAATAKHFPGLGAARENTDFAVQRIRLSKAALRKLDMAPY
                     KPYIASRGKLVMVGTAIYPALGPKPAAFEPKVVRGELRLRLLEARQHAGQRS"
BASE COUNT          500 a         1021 c          947 g          466 t
ORIGIN      
        1 ccctgcgagc ggggatgaag gctgacgtcg tcgaggatca gcggctccgg ttcctcgatc
       61 agcaggccga gcacgcctcc ccgtggggcg gatgcccgat cttctcgctc agtttctctt
      121 cgactccccg ggtcaggaac tgctcgagtt ccacccgccg ggtgtccagc acaccgagcg
      181 ccgcgtaccg ggctccggtc agctcgcggg ccgcttcgag ggcgtcggtg atgaccgctt
      241 cgggatcgga ttcgacgacg aggctgcggc cgacttcgat cagccttcgc agccgggctt
      301 cgtcgagact gaccgtcgtg tcttccacgg ccgggattct atggaccgct gccggtccgt
      361 ccaagtctgc ggaaaggtac ggaattggcg ccatttccgg cgggtagcct ctttctgtgg
      421 aatcaggtcc gcatagccct gccgaaaaac tgaccctggt gctggttgac gatcacgccg
      481 tggtgcgctc ggcgctcaag gtcctgctcg aatcggagcc cgatcttgag gtcgtcgccg
      541 aagccggcac cgccgatgac gcgatccgct atgtcggggc ccacaagccg gatgtgctgg
      601 tgctcgacct gaacatgccg ggccgaccca gcctcgaagc ggtccccgat attcaggctg
      661 cctcgcccga caccaagatc gtgatcctga ccatgcgaaa cgagccggcc ttcgcccgcc
      721 aggccctgcg ggccggggtg accggatacg tgctgaagga agccgccgat gccgagctgg
      781 tgctcgccgt ccgccgggcc gccgacggcg acacctacct gcagccttcg ctttaccaac
      841 acctggccga caaggacaaa gcggaagggc gcaccggccg caccctcagc gaccgcgagg
      901 tcgaggtaat gcgcctgatc gcgctcggcc acaccaacgc ggagatcgcc gaccagctct
      961 tcctcagcgt ccggaccgtg gagagccacc gggccagcat ccagaagaag ctggacatca
     1021 gcagccgcgc cgagctggtt cgttatgccc tcgaccaggg gctgatttcg gcttgagttt
     1081 cagggcccgg tcaacgggct cctgtcacga atagattccg cccgatgctc atcctgctgc
     1141 ccccatctga aggaaaggcc tcgccctccg gcaagacccg actcgatctt ggctccctcg
     1201 ctttcgccga ccagctcggc gggaagcggg ccgaactgat cgcggcgctc gagaagctcg
     1261 gaaaggctcc gcagaagaag gcgatcgagg tcctcggcat ctcgaaaggt caggcgggcg
     1321 agattgcccg cgatgcggaa ctggcaaccg cacccgcggc gccggcctcg gagctctaca
     1381 ccggggtgct gtacgaccgg ctcgacttcg ggaccctcac cgcggccgcc ggccgccggg
     1441 cgaagggaaa cctgctgatc gcctcagccc tctggggttt tctcgccccg gccgaccgaa
     1501 tcccctacta ccgcttttcg atgaaggcaa agctcccgaa gatcggcggc ctgccggcct
     1561 actggcgaga tgccctgacc gctgcgatgg aaagtgccgg ccacgacgag gagggaggcc
     1621 tggtgctcga catgcgctcc ggtgcctact cggccgcctg gaagccgaag cgggcccggc
     1681 tggtcaccgt gcgcggattc accgagaccg gcggcaagcg caaggcgatc tcccacatgg
     1741 ccaaggcggt ccgcggcgag gtcgcccgga ccgcgctctc ggcccgctcg atgccaaagg
     1801 acctggaaac cctggccggc ctgctcgaga aggaaggcaa gacgatcgaa ctgaccgaca
     1861 agaccctcga cgtgatcgaa aaggcctgag cccaggcaat tgagcccggc ccggaggggt
     1921 ccggagaggg ccggtgcgta tcatcgaatt gtggcttcca tcctgagaaa atcgcctgcg
     1981 ggtaccgcag taacggtcgt cctgctgctg gcgggactgg ccggtctcgt gctgctttcg
     2041 gcggctggcg acaactcggg agactcgaac gccactgccg gctcggcggc ggtcggaccc
     2101 ttcgtcaagt cggagaccgc cagctccccg tttccgccgc tgacccggaa cctttcagcc
     2161 gggctgggca taaatcaact cgccggccag cggatcgttg ccggtttcga gggcaagtcc
     2221 gttccgccgg tgatccggcg ccggatcagg gccggcctga tcggcggggt catcctcttc
     2281 gccgacaacc tcggttcacg cagtcagatc cagcggctga cccgctcgct tcagcgcatc
     2341 gggcgccccg caggcctgcg acgctatccg ctcatgatca tggtcgacca ggaaggcgga
     2401 ctggtcaaga ggctctccgg cgctccgaac gcgtcggccg agcagatggg tgccaggggc
     2461 gctgcctatt cgcgccgcca gggactgctg accggacgca acctgaagaa tgccggtttc
     2521 aacatcgatc tcgccccggt cctggacgtc gcccggcccg gcggggtgat cgccgacacc
     2581 gaccgcggat tcggctcgac cgtgaaaaag gtgaacgcca cggcgattcc cttcgcccag
     2641 gctcttcaga gaaccggcgt tgccgccacc gccaagcatt tccccggcct cggcgccgcc
     2701 cgggagaaca cggacttcgc ggtccagcgg attcgcctct cgaaggcggc actccgaaag
     2761 ttggacatgg ccccttacaa gccgtacatc gccagccggg gcaagctggt catggtcggc
     2821 actgcgatct acccggccct cgggccaaaa ccggccgcct tcgaaccgaa ggtagtccgg
     2881 ggcgagctcc gcctgcgcct cctcgaagcc cgacagcacg ctggtcagcg cagc
//
LOCUS       DAOTIP010000191         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40102_length_2932_cov_2.660410, whole genome shotgun sequence.
ACCESSION   DAOTIP010000191 DAOTIP010000000
VERSION     DAOTIP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40102_length_2932_cov_2.660410"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="P5138_04590"
     CDS             complement(<1..239)
                     /locus_tag="P5138_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59884.1"
                     /translation="MKAGDEPDEQAIADAYVYLLARMLVIRQEGIDASEEGFAYNKVK
                     YNPLGSAEFVNPNLDVAYLECWIAVDDDSVVLLTVP"
     gene            complement(256..1620)
                     /locus_tag="P5138_04595"
     CDS             complement(256..1620)
                     /locus_tag="P5138_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRV59885.1"
                     /translation="MNNPTKTSMPPGPGTPGRLNQALFLLARNRLFRRWHDQYGDIYF
                     LDIAGFGPSVVIRGAELHRKVFTAKDNVLHAGDNALGRLLGDGSIFSMDEEQHLAERR
                     KLLPPFHGERMRSYEKLVEDEANREMDTWPLDEPFPTQKSFNMITLRIILRAVFGAEG
                     RELSELEEIIPPMTRYGQMKVASDLLMRDYGRYSPGHRWESLTARFHEIIRVLIEKHL
                     ADPDLDERIDILALMLNSDLEQGHEIDFRATGEELMTILLAGHETTASSLAWTIERLT
                     RHPEVLDRLTAEARTDENDYRLAVINEVLRIRPVIGGTGRSVFREDFELDGWTLPAGT
                     RIICNIRDTNLDPEVYPEADRFNPERYLGKKPDTYSWIPFGGGMRRCIGASFAQFEMD
                     IVLRTLLRRFDLATTSRPPEREKFKGLAYAPAEGAVAMVRKAQPKVEELRPADLVGTE
                     SGQA"
     gene            complement(1617..2513)
                     /locus_tag="P5138_04600"
     CDS             complement(1617..2513)
                     /locus_tag="P5138_04600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV59886.1"
                     /translation="MSRIRGKVCVVTGAGSGIGRALAVELAKRGAKAVAISDIKADSV
                     ERTAELIGRGTEIHTATLDVSDREAFAAYAEEIAGHFGVVHQVCNNAGVADDDPVDVF
                     GYERYERILDVNLWGVIHGTKEFLPHLIASGDGHITNISSLNGIMAQQGLSAYCASKF
                     AVRGFTESVAFDLEEEGYPVRTTVVHPGGIKTGIADSALEWAKDTGVPVTAEKEARTR
                     LYNEKLLKMDPARAAEIIADGVESNNPRVLVGNDAKLLDALVRLMPRRWPKVFGLLQR
                     QIEKELETPAVETAPESELVHR"
     gene            complement(2510..>2932)
                     /locus_tag="P5138_04605"
     CDS             complement(2510..>2932)
                     /locus_tag="P5138_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing monooxygenase EthA"
                     /protein_id="HRV59887.1"
                     /translation="IDGETLDAPSTVAYKGMMLSDVPNLVFAIGYTNSSWTLKVDLVC
                     QYFCRLLGYMDEGHFDSAVARVSDPDMDRVPLLDFPAGYVLRALDELPRQGTKEPWYL
                     AQSYYKDSRFLRHGDVADEYMDFRRIEHKSEAREEVPA"
BASE COUNT          478 a          934 c          929 g          591 t
ORIGIN      
        1 ggaacggtca gcagcacgac cgagtcatcg tcgacggcga tccagcactc gagataggcg
       61 acatcgaggt tcgggttcac gaattcggcc gaacccagcg ggttgtactt caccttgttg
      121 taggcgaagc cctcctcgga cgcgtcgatc ccctcctgcc ggatcaccag catacgggcg
      181 agcaggtaga cgtatgcgtc ggcgatcgcc tgctcgtcgg gttcgtcccc tgccttcact
      241 tgtcaggtga gcaggtcagg cctgaccgga ttcggtcccg accaggtccg ccgggcgcag
      301 ttcctccacc ttcggttgcg ccttcctgac cattgccacg gccccttctg ccggtgcgta
      361 ggcgagtccc ttgaactttt cccgctccgg cggcctgctc gtggtggcga ggtcgaagcg
      421 gcgaagcagc gtgcgcagga cgatgtccat ctcgaactgg gcgaaggacg caccgatgca
      481 gcggcgcata ccgccgccga agggaatcca ggagtaggtg tcgggcttct tgccgagata
      541 gcgctcgggg ttgaaacggt cggcctcggg gtacacctcg ggatccaggt tcgtgtcgcg
      601 gatgttgcag atgatccggg tgccggccgg aagcgtccag ccatcaagtt cgaagtcctc
      661 cctgaaaacg gagcggccgg ttccgccgat caccggccgg atcctcagga cctcgttgat
      721 cacggccagc ctgtagtcgt tctcatcggt ccgggcctcg gcggtgagac gatccagcac
      781 ctccgggtga cgggtcagcc gctcgatggt ccaggccagg gaggacgcgg tggtttcgtg
      841 gccggcgagc aggatcgtca tgagctcttc cccggttgcc cggaagtcaa tttcatgtcc
      901 ctgctcgagg tcgctgttca gcatcagggc gagaatgtca atccgctcat cgaggtccgg
      961 atcggccagg tgcttctcga tcaggacccg gatgatttcg tggaaccttg cggtcaggct
     1021 ttcccagcgg tggccagggc tgtacctgcc gtagtcgcgc atcagcagat cggaagccac
     1081 cttcatctgg ccgtagcggg tcatcggggg gatgatctct tcaagttccg acaactccct
     1141 gccttcggcg ccgaagaccg cgcggagaat gatccgcagg gtgatcatgt tgaaggactt
     1201 ctgggtcggg aagggctcat cgagcggcca cgtgtccatc tcgcggttgg cttcgtcctc
     1261 gaccagcttc tcgtacgagc gcatccgctc tccgtggaac ggtggcagca gcttgcggcg
     1321 ctcggccagg tgctgctcct cgtccatcga gaagatcgag ccgtccccca ggagcctgcc
     1381 gagagcgttg tcaccggcgt ggagcacatt gtccttggcg gtgaacacct ttcggtggag
     1441 ttcggcgccc cggatcacga ccgacggtcc gaagccggct atatcgagga agtagatgtc
     1501 cccgtactgg tcgtgccagc ggcggaagag gcggttccgg gcaagcagga acagggcctg
     1561 attcagcctc cccggagtcc cggggccggg cggcatggat gtcttggtcg ggttgttcat
     1621 cggtgcacca gttctgattc aggggccgtc tcgacggccg gtgtttccag ttctttctcg
     1681 atctgcctct ggagcagccc gaaaaccttc ggccatcgcc gaggcatcag tcgcaccagg
     1741 gcatccagca atttggcgtc gttgccgaca agcacgcgcg ggttgttgct ttccaccccg
     1801 tcggcgatga tctccgctgc ccgggccgga tccatcttca gcagcttctc gttgtagagc
     1861 ctggtccgtg cttccttctc cgcggtgacc ggtactccgg tgtccttcgc ccattcgagt
     1921 gctgaatccg cgattcccgt cttgatcccg cccggatgga ccaccgtggt cctgaccggg
     1981 tagccctcct cctccaggtc gaaggcgacc gattcggtga agccgcgcac cgcgaacttg
     2041 ctggcgcaat aggcactgag tccctgctgg gccatgatgc cgttgagact ggaaatgttc
     2101 gtgatgtgcc cgtcaccgga ggcgatcagg tgcggaagga actccttggt tccgtggatc
     2161 actccccaca ggttcacgtc gaggatccgt tcgtagcgct cgtagccgaa gacgtcgacc
     2221 ggatcatcgt cggcgacgcc ggcgttgttg cagacctggt gaacgacccc gaaatgaccg
     2281 gcgatctcct ccgcgtaggc ggcgaaggct tcccggtcgc tcacgtcgag agtcgccgtg
     2341 tggatttcgg tgccgcggcc gatcagttca gcggtccgtt cgaccgaatc tgccttgatg
     2401 tcgctgatcg cgaccgcctt cgctccccgc ttcgccagtt cgacggccag ggcccggccg
     2461 atgccggacc ccgcgccggt gacaacgcag acctttcctc tgatcctgct caagccggga
     2521 cctcctccct ggcttccgac ttgtgctcga tgcgtcggaa atccatgtac tcgtcggcga
     2581 catcgccatg ccgcaggaag cgtgaatcct tgtagtaact ctgggccagg taccagggct
     2641 ctttcgtgcc ctgccgtggc agttcgtcaa gagcccggag aacgtatcca gccgggaagt
     2701 caagcagcgg gacccggtcc atgtccgggt cgctgactct cgccacagcc gagtcgaaat
     2761 ggccctcgtc catgtacccg agcagccggc agaagtactg gcagaccaga tccaccttca
     2821 gggtccacga ggagttggtg taaccgatcg cgaagaccag gttcggcacg tcgctcagca
     2881 tcatgccctt gtaggccacg gtgctcgggg catcgagggt ctctccgtcg at
//
LOCUS       DAOTIP010000192         2922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40276_length_2922_cov_3.633764, whole genome shotgun sequence.
ACCESSION   DAOTIP010000192 DAOTIP010000000
VERSION     DAOTIP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40276_length_2922_cov_3.633764"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="P5138_04610"
     CDS             <1..415
                     /locus_tag="P5138_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRV59888.1"
                     /translation="SRKDQTYNTPALATLYLLAEQLDWMLAGGGLDFCVERTRASSSH
                     LYGWAEASDFATPFVTDPAQRSLVVGTIDFDESIDAAAVAATLRANGIVDVEPYRKLG
                     RNQLRIAMFPAVDPSDIEALTACVDWVIANAGVTA"
     gene            468..2060
                     /gene="serA"
                     /locus_tag="P5138_04615"
     CDS             468..2060
                     /gene="serA"
                     /locus_tag="P5138_04615"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRV59889.1"
                     /translation="MSDRPKVLVKEKIADSGVEQLKENFDVEIGVDMDDTELAERIGE
                     FDAILIRSATKMTPELIGKADNMKVIGRAGTGVDNVDIPAATKRGIIVANAPESNSVA
                     AAEHTMALALALFRNVPQAHSALMAGRWDRAKYKGAELYGKTLGVVGFGRIGQLVAKM
                     AQGFDMEVLAFDKFVSHERFRDLGVEGVDSPEEMYRRADLITIHLPKTAETVDWIDAE
                     AVSKFKPGMRIVNCARGELVNLDALIAGLDSGQLAGAALDVFPEEPFTEHPVFSREDV
                     VVTPHLGASTAEAQDRAGTDTAEQVTAALTGGVVTNAVNIPAVSPAVMEALAPYVPLC
                     QTLGRLAVGLTPGSIDRVEVEFRGGIADNDTRLLGIAVQAGLLSGHTEEAVNLVNAPH
                     MAEERGIEVIEIKDSTADEFTDLVRVTIQSGSDVVTVGGTTVGPKNVPYLVSIWGQSF
                     YLPFAEHMAVFRYADQPGMIGKVGSLFGEQGVNIGSAAVGAESGSHNAVMVVTSDAPV
                     SAETIETIKAVDGFSAGYAIEL"
     gene            2092..>2922
                     /locus_tag="P5138_04620"
     CDS             2092..>2922
                     /locus_tag="P5138_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRV59890.1"
                     /translation="MRAVVLTGHDGPDDLKVEQRPDPEAGPGEVRVAVEAAGINFADV
                     MATLGLYPDAPGTPCIIGYEFAGEVEAIGPGVTGISEGDRVFGPTMFGGYAEKVTVPA
                     GDIYPIPEGFSAEEAAALPVNYCTALAALVVMGGLREGQRVLIHAAAGGVGIAATQIA
                     REAGAEIFGTASAPKHEAIRAQGVDHAVDYRNRDFAEEVRRLTGGEGVDLIIDATGPT
                     NFRKDYRLLRPGGKLVMYGASELAEGKGVFDRRTLSALVRMPLAAIPWWKSFAVMNEN
                     K"
BASE COUNT          518 a          939 c          977 g          488 t
ORIGIN      
        1 ctcccgcaag gaccagacct acaacacgcc cgcgctcgcg accctgtatc tgcttgccga
       61 gcagctcgac tggatgctcg ccggcggtgg actcgatttc tgcgtcgagc ggacccgcgc
      121 ttcgtcgtcg cacctgtacg ggtgggccga ggccagtgat ttcgcaaccc ccttcgtcac
      181 cgatcctgcc cagcgctcac tcgtggtcgg aacgattgac ttcgacgagt cgatcgacgc
      241 cgccgccgtc gcggcgacgc tgcgcgcgaa cggcatcgtc gatgtcgagc cctaccgcaa
      301 gctcggccgc aaccagctgc ggatcgcaat gttcccggcg gtcgacccct ccgacatcga
      361 ggcgctcacg gcctgtgtcg actgggtgat cgcaaacgcc ggggtcaccg cgtgaaagtt
      421 ttcacttacg ccggttcaac cgagccggac aaccaggagg ttccattttg agcgacaggc
      481 ccaaggtact cgtcaaggag aagatcgccg acagcggcgt cgagcagctg aaggagaact
      541 tcgacgttga aatcggcgtc gacatggacg acaccgaact ggccgaaagg atcggcgagt
      601 tcgacgcgat cctgatccgc tcggcgacga agatgacgcc ggagctgatc gggaaggccg
      661 acaacatgaa ggtgatcggc cgggccggca ccggcgtcga caacgtcgac attccggccg
      721 ccaccaagcg gggcatcatc gtcgccaacg ctcccgaatc gaactccgtt gccgccgccg
      781 agcacaccat ggccctcgct ctcgctcttt tccgcaacgt tccccaggcc cattcggccc
      841 tgatggccgg acgctgggac cgggccaagt acaagggcgc cgagctttac ggaaagaccc
      901 tcggtgttgt cggctttggc cggatcggcc agcttgtcgc gaagatggcg cagggcttcg
      961 acatggaggt tcttgccttc gacaagttcg tatcccacga gcgtttccgt gatctcggcg
     1021 ttgaaggggt cgattcaccc gaggagatgt atcgtcgggc ggacctgatc acgatccacc
     1081 tgcccaagac ggcagagacg gtcgactgga tcgacgccga ggcggtctcg aagttcaagc
     1141 ccggcatgcg catcgtcaat tgcgcccgtg gcgagctggt caacctcgac gccctcatcg
     1201 ccgggctcga ctccggtcag ttggccggcg ctgctcttga cgtgttcccg gaggagccgt
     1261 tcaccgaaca tcccgtcttc tcccgcgagg acgtcgtcgt gaccccgcac ctcggggcct
     1321 cgactgcgga ggcccaggac cgggccggta ccgacacggc cgagcaggtg accgcagcgc
     1381 tgaccggcgg cgtggtgacc aatgcggtga acatccccgc ggtgagcccc gcagtgatgg
     1441 aggccctcgc accttatgtg ccgctctgcc agacgctcgg ccggctcgcg gtcggactca
     1501 cgccgggctc gatcgaccgg gtcgaggtcg agttccgcgg aggcatcgcc gacaacgaca
     1561 cccgtctgct cggcatcgcg gtccaggccg gcctgctctc gggtcatacc gaggaagcgg
     1621 tcaacctcgt caacgctccc cacatggccg aggagcgggg catcgaggtg atcgaaatca
     1681 aggactcgac cgcggacgaa ttcaccgacc tggttcgtgt cacgatccag tccggctccg
     1741 acgtggtcac ggtgggcggc acgacagtag gtccgaagaa cgtgccctac ctcgtttcga
     1801 tctggggaca gagcttctat ctcccgttcg ccgagcacat ggccgttttc cggtacgcag
     1861 accagccggg catgatcggc aaggtcggtt cgctgttcgg cgagcaggga gtcaacatcg
     1921 gttcggctgc ggtcggtgcc gagtccggca gccacaacgc cgtcatggtg gtcacctccg
     1981 acgctccggt gtcggcggag acgatcgaga cgatcaaggc agtcgatggc ttctcggccg
     2041 gttacgcgat cgaactctag gcatccggac gcgactggtt aggctggggc catgagagcc
     2101 gtggtcctga ccggtcacga cgggccggac gacctgaagg tcgagcagag gccggatccc
     2161 gaggcagggc ccggtgaagt cagggtggcg gtcgaggcgg cggggatcaa cttcgccgac
     2221 gttatggcca ccctggggct ctaccctgac gcacccggaa cgccctgcat catcgggtac
     2281 gagttcgcgg gggaggtcga ggccatcggt cccggggtca caggcatatc tgaaggtgac
     2341 cgggttttcg ggccgacgat gttcggcgga tacgccgaga aggtgacggt tccggccggt
     2401 gacatctacc cgatcccgga agggttctcg gccgaggagg cggcggctct tccggtcaac
     2461 tactgcacgg ccctggcggc actggtcgtc atgggcggcc tgcgggaggg ccagcgggtc
     2521 ttgattcacg ccgccgccgg aggagtcggg atcgcggcca cccagatcgc ccgagaagca
     2581 ggggccgaga tatttggcac ggcttcggca cccaagcatg aggccatccg cgcgcagggg
     2641 gtagatcatg cggtcgatta ccggaatcgc gatttcgcgg aggaagtccg gcgactgacc
     2701 ggaggggagg gggtcgacct gatcatcgat gcgaccggcc cgaccaactt ccgcaaggac
     2761 tatcggctgc tcaggccggg cggcaagctg gtgatgtacg gagcctccga actggctgaa
     2821 ggaaagggcg tcttcgaccg tcgcacgctc tcggcactgg tgcggatgcc ccttgccgcc
     2881 atcccctggt ggaagagctt cgcggtgatg aacgagaaca ag
//
LOCUS       DAOTIP010000193         2920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40307_length_2920_cov_2.956719, whole genome shotgun sequence.
ACCESSION   DAOTIP010000193 DAOTIP010000000
VERSION     DAOTIP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40307_length_2920_cov_2.956719"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="P5138_04625"
     CDS             complement(<1..563)
                     /locus_tag="P5138_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRV59891.1"
                     /translation="MSESKKIVVLAGDGIGPEIVGATRRVLDAVGDFEYTDHLIGGAS
                     IDEHGVALTDEVIEACKGSDAVLLGAVGGPKWDEAVAKDPTAPRPEQGLLGIRKALGL
                     YANLRPVKPSPALIDASPLKKELIDGTDLLVVRELTGGIYFGDSGRREDGQVAYDECA
                     YSVAEIERITRAAFDAARQRSDSPRVLS"
     gene            complement(556..1341)
                     /locus_tag="P5138_04630"
     CDS             complement(556..1341)
                     /locus_tag="P5138_04630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59892.1"
                     /translation="MSDQESRPADWDADKYSRVATPQREWSGAVLDRLVLNGDETVMD
                     AGCGSGNVTEDLLAKLPNGRVIGVDGSPSMVEEATERLGEDPRTTFICQDLARLRLDE
                     QVDHAFSNATFHWIGDHDSLFRSLARATKPGGSLVAQCGGRGNVAEVIAALGVVNRQE
                     PFAELFGDYEDPWNFAGPEETEIRLVDAGYEDVECWLEERVAHPEDPRGFFEASFLAP
                     AQQTLDPDLFTEYTDRLLGEMGGPTSFNYVRLNMTAKRKNENE"
     gene            complement(1355..1918)
                     /gene="leuD"
                     /locus_tag="P5138_04635"
     CDS             complement(1355..1918)
                     /gene="leuD"
                     /locus_tag="P5138_04635"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRV59893.1"
                     /translation="MEAISTIEGEVAFLDRSDVDTDQIIPKQFLKSIERTGYGEFLFY
                     DWMRDGEIELEAHPILVSGRNFGSGSSREHAVWALHQFGFQAVIAPSFSDIFYSNSTK
                     NGFLPVVLPEEACREIATSGEAKIDLESQTVSWDGGEASFEIDAEIKRRLLGGLDDIA
                     LTLQSEDEIEAFEAGSGGTYGPVTTSL"
     gene            complement(1921..>2920)
                     /locus_tag="P5138_04640"
     CDS             complement(1921..>2920)
                     /locus_tag="P5138_04640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRV59894.1"
                     /translation="THGAFGALAFGIGTSEVEHVMATQTLVQNKPKTMRINYSGTLGE
                     GVTSKDLILATIGKLGTSGMTGYVVEYAGEAIEALTMEQRMTICNMTIEGGGKAGMIA
                     PDETTFDYMRDKPGVPEDFDAAVERWRLLPTDDGASFDTEVDIDAGSISPMVTWGTTP
                     GMVIQVTDSVPDPEMMDSPADKEAAERALQYMGLEAGTPMEEVRPERVFIGSCTNSRI
                     SDLREAAGAIKGRKVAGSVRAMVVPGSALVKAQAEEEGLDEIFREAGFEWRGAGCSMC
                     LGMNPDILIEGERCASTSNRNFEGRQGKGGRTHLVSPTMATAAAIEGHFVDIRNWS"
BASE COUNT          480 a          978 c          900 g          562 t
ORIGIN      
        1 gaaagaaccc gcgggctgtc tgagcgctga cgggcagcgt cgaaagccgc tctggtgatc
       61 cgctcgatct cggcgaccga gtaggcgcat tcgtcgtagg cgacctgtcc gtcttcccga
      121 cggcctgaat ccccgaaata gatgccgccg gtcagctccc gaacgaccag aaggtcggtc
      181 ccgtcaatca gctccttctt caaggggctg gcgtcgatca gagccggcga gggcttgacc
      241 ggacgcaggt tcgcgtagag ccccagcgcc ttgcggatgc cgagcagacc ctgctcaggt
      301 cgcggagcgg tcgggtcctt ggcgaccgcc tcatcccact tcggaccgcc gaccgcgccg
      361 agcaggacag cgtcggagcc cttgcatgcc tcgatcacct cgtcggtcag ggcgacaccg
      421 tgctcatcga tggaggcacc gccgatcagg tgatcggtgt actcgaaatc tccgaccgcg
      481 tcgagaaccc tccgggtcgc gccgacgatc tccgggccaa tgccatctcc ggccaggacg
      541 acgatcttct tgctctcact cattctcgtt cttccttttc gcggtcatgt tcaggcgaac
      601 atagttgaag ctggtcggac cgcccatctc gcccagcaga cggtcggtgt attcggtgaa
      661 taggtccggg tccagagtct gctgtgcggg cgccaggaag gacgcctcga agaagccccg
      721 cggatcctcc ggatgggcga ccctctcctc aagccagcac tcgacgtctt cgtagccggc
      781 gtcgaccagc ctgatctcgg tctcctccgg acccgcaaaa ttccacggat cctcgtaatc
      841 gccgaacagc tccgcaaagg gctcctgccg gttgacgacg ccgagcgcgg cgatcacctc
      901 ggccacgttc ccgcggccgc cgcattgcgc gacaaggctg ccgccgggct tggttgcccg
      961 tgccagcgaa cggaacagcg aatcgtggtc gccgatccag tgaaaggtcg cgttggagaa
     1021 ggcatggtcc acctgctcgt ccagccggag acgggccagg tcctggcaga tgaatgtggt
     1081 ccgggggtct tcaccaagcc tttcggtcgc ttcctcgacc atgcttgggg atccatcgac
     1141 gccgatgacc cgcccgttcg gcagcttcgc cagcaggtct tcggtcacgt tgcccgatcc
     1201 gcagccggcg tccatcacgg tctcgtcacc gttcaggacc agacggtcga gaaccgcgcc
     1261 ggaccattcg cgctgcggag ttgccacgcg ggaatacttg tccgcatccc agtccgccgg
     1321 acgggattcc tgatcgctca tgtcagatcg tctcctagag ggacgtagtc acgggaccgt
     1381 aggtcccgcc ggagccagcc tcgaaggcct cgatctcatc ctcagactgg agagtgagcg
     1441 caatgtcgtc gagcccgccg agcagacggc gcttgatttc agcgtcgatc tcgaaggatg
     1501 cctctccccc gtcccagctc acggtctggc tttcgaggtc gatcttcgct tcgccggagg
     1561 tcgctatctc gcggcaggcc tcttcgggca gcacaaccgg caggaagccg ttcttggtgc
     1621 tgttggaata gaagatgtcc gagaaggacg gagcgatcac ggcctggaag ccgaactggt
     1681 ggagcgccca gacggcatgc tcacgggaag acccggaccc gaagttccgg ccggagacca
     1741 ggatcggatg ggcttcgagt tcgatttccc cgtcccgcat ccagtcgtag aagagaaact
     1801 cgccgtatcc ggtccgctcg atcgacttca ggaactgctt ggggatgatc tggtcggtgt
     1861 cgacgtcgct gcggtccagg aaggcgactt cgccttcgat tgtgctgatc gcttccattt
     1921 ctagctccag ttcctgatgt cgacgaaatg accctcgatg gccgctgccg tggccatggt
     1981 cgggctgacg agatgggtac ggccgccctt gccctgacga ccttcgaagt tccggttcga
     2041 ggtcgaggcg cagcgctcgc cttcgatcag gatgtcgggg ttcatgccga ggcacatcga
     2101 gcagccggcg ccccgccact cgaatccggc ctcacgaaag atctcgtcga ggccttcttc
     2161 ctcggcctgc gccttgacca gcgccgatcc gggcacgacc atcgcccgca cgctcccggc
     2221 gaccttgcgg cccttgatcg ctccggctgc ttcccgcagg tcggagatcc tcgagttggt
     2281 gcaggagccg atgaagaccc gttcgggacg gacctcctcc atcggggttc cggcctccag
     2341 ccccatgtac tgcagggcgc gttcggccgc ctccttgtcc gccggcgagt ccatcatttc
     2401 cggatccggc actgaatcgg ttacctggat gaccatgccg ggggtggtac cccaggtcac
     2461 catcggcgag atgctcccgg catcgatgtc gacttcagtg tcgaagctcg ccccgtcatc
     2521 ggtcggcagc agcctccagc gttcgaccgc ggcatcgaaa tcctccggca caccgggctt
     2581 gtcccgcatg tagtcaaagg tggtctcgtc cggagcgatc attcctgcct tgccgccgcc
     2641 ctcgatggtc atgttgcaga tggtcatccg ctgctccatg gtcagggcct cgatcgcctc
     2701 tcccgcgtac tcgaccacgt agccggtcat gccggaggtt cccagcttgc cgatcgtggc
     2761 gaggatcagg tctttggagg tgacgccctc accgagcgtt cccgagtagt tgatccgcat
     2821 ggtcttcggc ttgttctgga ccagggtctg ggtcgccatc acatgctcga cctccgaggt
     2881 tccgatgccg aaggcgagcg cgccaaaggc gccgtgggtc
//
LOCUS       DAOTIP010000194         2917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40357_length_2917_cov_3.296296, whole genome shotgun sequence.
ACCESSION   DAOTIP010000194 DAOTIP010000000
VERSION     DAOTIP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40357_length_2917_cov_3.296296"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="P5138_04645"
     CDS             complement(<1..799)
                     /locus_tag="P5138_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59895.1"
                     /translation="MGICSTGSLIALVFGLLTAATPITAAAAPAHGSSAAMKLAEKYV
                     PVMMIRKQGKDLCDTEVEQYQVMKVDALFGNPDVRLMRNNGSDKSTLIKRAPTIRDVT
                     NRGEEAYIDLPGDPLGDTCVYSKDFAAMKADGKAPVAVYAHIARQEGRSGLALQYWFY
                     WYFNQFNDLHESDWEGMQLTFDADTPAAALKEEPSQMILFQHAGGERANWHDGKVEKE
                     GNHPVVYPAAGSHATFYQSAVFPQNGSHGSGVGCDNTSEPLRELRPVP"
     gene            844..1710
                     /locus_tag="P5138_04650"
     CDS             844..1710
                     /locus_tag="P5138_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV59896.1"
                     /translation="MSGNFAAMRSDVPETVDLFHFDQPGAIATHLFDGVIVDPGAEKT
                     VDRLLEALGDDVPEAILLTHIHFDHAGATGRLVEKFPDVEVWVHEAGAPHMIDPERLV
                     SSARKVFGDHFDMMWGEVVPVPEGNIRVLRGGESDGPWKVEYTPGHAKHHVCYLHAPT
                     GTAFTGDVTGVRIEGGPAFPPTPPPDIDPPLWHESLETVRAWQPERVVFQHFGQATDV
                     DEQIDMMHESLDLFAGKARETDAEGMSAWIRQWLGEQVGEAQLNTYWRAGPFEGMWSG
                     LDRYWQKQQSGS"
     gene            complement(1717..2631)
                     /locus_tag="P5138_04655"
     CDS             complement(1717..2631)
                     /locus_tag="P5138_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV59897.1"
                     /translation="MSAADLRAWRERGRMVAATPLGHRAFLIDEGNPDASPDSTLVLL
                     HGFPESSYSYAGAFPLVAGVFDRVVSFDFIGFGFSDKPADGFGYSLMEQADVALRLIR
                     ESGIEGCHFIAHDMGDSVMTELLARINQNQPSWFGTGAVSVSFTNGGMVLGDAKLRVG
                     QRALVSPAGRMLSRIMDSPTVFEKQVNSAQGNDRLTEEQILDMYSASSSGAPRGLMAE
                     LIGYLRERRRFEKSRWLPAVRDATMPIQICWGADDAVAPVAIARRLQSEVKPEARLTV
                     IDGLGHFAQIQDPDAWFGGLAPFWEEIA"
     gene            complement(2718..>2917)
                     /locus_tag="P5138_04660"
     CDS             complement(2718..>2917)
                     /locus_tag="P5138_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRV59898.1"
                     /translation="RATRKGDTMSEHRTEDAKGRAKEAAGAVTGDKDLKKEGKADQAS
                     ASAKGKLESAVDKVKDKITGN"
BASE COUNT          504 a          981 c          913 g          519 t
ORIGIN      
        1 ccggcaccgg ccgcagttcc cggaggggct ccgacgtgtt gtcgcatccc accccggaac
       61 cgtgggaacc gttctggggg aagacggccg actggtagaa ggtcgcgtga gaaccggctg
      121 ccgggtaaac gaccgggtga ttcccctcct tctccacctt gccgtcgtgc cagttggcgc
      181 gctccccgcc ggcgtgctgg aagaggatca tctgggaggg ttcttccttg agcgcggcgg
      241 ccggcgtgtc cgcgtcaaaa gtgagctgca tcccctccca gtcgctctcg tgaaggtcgt
      301 tgaactggtt gaagtaccag tagaaccagt actggagcgc cagacccgat cgtccctcct
      361 gcctggcgat gtgggcatag accgcgaccg gcgccttgcc gtcggccttc atcgcggcga
      421 agtccttcga gtacacgcag gtgtccccga gcgggtcacc tggcaggtcg atgtacgcct
      481 cctcgccccg gttggtcacg tccctgatcg tcggtgcccg tttgatcagg gtcgacttgt
      541 cactgccgtt gttgcgcatc agcctgacgt cgggattgcc gaaaagggca tcgaccttca
      601 tcacctggta ctgctcgacc tcggtgtcgc agaggtcctt gccctgcttg cggatcatca
      661 tcaccggcac gtatttctcg gcaagcttca tcgccgcaga agagccgtgt gccggagcag
      721 ccgcggctgt tatcggcgtg gctgccgtca gcaacccgaa gaccagtgcg atcagcgagc
      781 cggtcgagca aatccccaaa cgagattttc ctccggacgg cacgaacaca ggctattggt
      841 tcgatgtccg gtaatttcgc ggcaatgcga agcgacgtcc cggaaaccgt tgacctcttc
      901 cacttcgacc agccgggtgc catcgcgacc catctcttcg acggagtgat cgtcgacccg
      961 ggggccgaga agacggtgga ccggctgctc gaggcgctcg gagacgacgt tcccgaagcc
     1021 atcctgctca cccacatcca cttcgatcac gccggtgcga cgggccgcct ggtcgagaag
     1081 ttccccgacg tcgaggtctg ggtgcatgag gcaggggcac cgcacatgat cgacccggaa
     1141 cggctggtca gcagcgcccg gaaggtcttc ggcgaccact tcgacatgat gtggggcgaa
     1201 gtcgtcccgg taccggaagg caacattcgc gtgctcagag gcggcgaatc ggacggaccc
     1261 tggaaggtcg agtacacgcc cggccacgcc aagcatcacg tctgttacct gcatgcgccg
     1321 accggcaccg ccttcaccgg cgacgtcacc ggggtacgga tcgaaggcgg accggccttc
     1381 cctcccactc cgcctcccga catcgacccg ccactgtggc atgagtcact ggagacagtc
     1441 cgcgcctggc aaccggagcg ggtcgtattc cagcatttcg gacaggcgac cgacgtcgat
     1501 gagcagatcg acatgatgca cgaatcgctg gacctctttg ccgggaaggc ccgcgaaacc
     1561 gacgccgagg gcatgtccgc ctggatccgt cagtggctcg gcgaacaggt cggcgaggcg
     1621 cagctcaaca cctactggcg agccgggccc ttcgagggga tgtggtccgg gctcgaccgc
     1681 tactggcaga agcagcagtc cggttcctga ccggactcag gcgatctctt cccagaaggg
     1741 cgcgaggccg ccgaaccagg cgtcgggatc ctggatctgc gcgaaatgcc caaggccgtc
     1801 gatcaccgtg agcctggctt ccggcttcac ctcgctctga agccttctgg cgatcgcgac
     1861 cggggcgacc gcgtcgtccg ccccccagca gatctggatc ggcatcgtgg catcccggac
     1921 cgccgggagc cagcgggact tctcgaagcg gcggcgttcc cgcaagtaac cgatcaattc
     1981 ggccatcaag ccgcgtggcg cgcccgacga agaagccgaa tacatgtcaa gaatctgctc
     2041 ttcggtaagc cggtcgttgc cctgggcgct gttcacctgc ttctcgaaga cggtcgggct
     2101 gtccatgatc cgggagagca tccggccggc cggtgagacc agggccctct gccccaccct
     2161 cagcttcgcg tcgccaagca ccatgccgcc gttggtgaaa ctgacgctca cggcaccggt
     2221 gccgaaccag gacggctggt tctggttgat ccgcgcgagc agttcggtca tcaccgagtc
     2281 acccatgtcg tgggctatga agtggcaacc ctcgatcccg gattcgcgga tcagccgcag
     2341 cgccacatcg gcctgctcca tcagcgaata cccgaagccg tcggccggtt tgtcggagaa
     2401 gccgaagccg atgaagtcga aggaaacgac ccggtcaaag accccggcca cgagcgggaa
     2461 cgccccggcg taggaatagg acgattccgg aaacccgtga agcagaacca gcgtcgaatc
     2521 cggcgacgcg tcggggttgc cctcgtcaat caggaaagcc cgatggccca gcggcgttgc
     2581 ggcaaccatg cggccgcgtt cgcgccaggc cctgagatcg gcggcactca cgacgcgccc
     2641 ggaaactcga cccgaatcat gatccggatc ctagcccgtg ggccgggcgg ccccctagag
     2701 gcaggtccgg acgggaatca gttcccggtg atcttgtcct tcaccttgtc gacggcgctt
     2761 tccagcttcc ccttcgccga cgcggaggcc tggtctgcct tgccctcctt cttgaggtcc
     2821 ttgtcgccgg tcacggcgcc ggccgcttcc ttggcccggc ccttggcatc ttctgtcctg
     2881 tgttcgctca tcgtgtctcc ttttctggtt gccctcg
//
LOCUS       DAOTIP010000195         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40501_length_2909_cov_3.412404, whole genome shotgun sequence.
ACCESSION   DAOTIP010000195 DAOTIP010000000
VERSION     DAOTIP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40501_length_2909_cov_3.412404"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="P5138_04665"
     CDS             <1..1168
                     /locus_tag="P5138_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRV59899.1"
                     /translation="AFQAVKDYVADPALAPPLVALAVCFLMLAGARRRPVFPFSLLGV
                     VLATVASAVFGLDLAPIGDLPAGLPAPSLGFIDFGSVTQLLPAVVAVTALAALESLLS
                     ATVADGMSVGEQHDPDRELFGQGLANLAAPVFGGIPATAAIARTAVNVRAGAGSKLAA
                     LTHALILLAIILVAAPLVSKIPLAALAGVLLATTVRMVEVGSLRAMARATRGDAMVLV
                     LTFAITVIFDLVAAVVVGLGVAIVIALRSVAQSASLEEVPLDHADHSREERELLSQHI
                     VAYRLDGPLFFAAAHQFLLELAEVADVRVVILRMARVSTIDATGAQVLGDAIGKLEQR
                     GIVVLISGISEDHSDVLDRLGAASHLRDSGRVFGDTPSAIRYARNLALADPIPA"
     gene            complement(1237..2427)
                     /locus_tag="P5138_04670"
     CDS             complement(1237..2427)
                     /locus_tag="P5138_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HRV59900.1"
                     /translation="MNRTFVIGVGMTKFDKPGTKEGDYPDWAVEAGQKALDDAGISYD
                     EVESAYVGYCYGEPTSGERAVYGLGLSGIPIVNVNNNCSTGSSALYLARQSVKGGLAD
                     CALALGFEKMEKGSLGAKYTDRTQPLDKHFIELTQLRQWEDAPPAPQIFGAAGREHME
                     KYGSKPEHFAWIGFKNHKHSVNNPYAQFQDEYTLQEIQEAPEIYSPLTKLQCSPTSDG
                     SGAAIVASEKFVDEHGLWDKAIEITGQALVTDTKETFDDDSAISLVGFEMSRLAAEKA
                     LNEAGTGIDEVDVVELHDCFSANELITYEALGLAERGHGHELVDAEATTYGGNTVVNP
                     SGGLISKGHPLGATGLAQCSELTWQLRGDADARQVEGADKALQHNIGLGGAAVVTVYE
                     KPTG"
     gene            complement(2448..>2909)
                     /locus_tag="P5138_04675"
     CDS             complement(2448..>2909)
                     /locus_tag="P5138_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59901.1"
                     /translation="TPSSNSLTNSVPVDRAGMASGTADLQRDLGGALMQSILGSVLTS
                     AYASSFASQIAGNPQASQQVSESTQTQLERSFGSAVTVAEQHPQYAPQIVHAAQTAFL
                     NGSDSAYVVGIAAIILGALVVFFAFPRKDHEVELLAQFHAEDAAATAPATN"
BASE COUNT          412 a          964 c          974 g          559 t
ORIGIN      
        1 ggcattccag gccgtcaagg actacgtcgc ggacccggcg ctggcgcccc cgctggtcgc
       61 gttggcggtc tgtttcctga tgctggccgg ggcaaggcgc cggcccgtct ttcccttttc
      121 gcttctgggc gtggtccttg ccaccgtggc ttcggcggtc ttcggactgg accttgcacc
      181 gatcggcgat ctgcctgccg gactgccggc gccttcgctg ggattcattg acttcggcag
      241 cgttacccag ctgcttccgg ccgtggttgc ggtcaccgcc ctggcggcgc tcgaaagcct
      301 gctttccgcc accgtggccg acggaatgag cgtgggagag cagcatgacc cggaccggga
      361 actcttcggg caggggctgg cgaacctcgc agcaccggtc ttcggcggca ttcccgccac
      421 cgcggcaatc gcccgcactg ccgtcaacgt ccgggccggg gccggatcaa agctggccgc
      481 cctgacccac gcgctgatcc tgctcgccat catcctcgtc gctgctcccc tggtctcgaa
      541 gatcccgctc gccgccctgg ccggagtgct actcgcgacc acggtgcgga tggttgaagt
      601 cggttcgctc cgggcgatgg cccgcgccac gcgaggcgac gcgatggtgc tcgtcctgac
      661 cttcgcgatc accgtgatct tcgatctggt cgcggcggtc gtggtcggac taggagtggc
      721 gatcgtgatc gcgctgcgct cggtcgcgca gagcgccagc ctcgaagaag tcccgctcga
      781 tcacgccgac cactcgaggg aggagcggga gctgctcagc cagcacatcg tcgcctaccg
      841 gctggacggc ccgctcttct tcgcggccgc ccaccagttt ctcctcgagc tggccgaggt
      901 cgccgatgtg agggtggtca tcctccgcat ggcccgggtc tcaaccatcg acgcaaccgg
      961 tgcccaggtg ctcggcgacg cgatcggcaa gctggaacag cgcggcatcg tggtgttgat
     1021 ctcgggcatc tccgaagatc acagcgacgt ccttgaccgt ctcggcgcag cgtctcacct
     1081 cagggatagc ggccgggtat tcggcgacac gccgtcggcc atccgctacg cccgcaacct
     1141 tgcgctggcc gatccgatcc cggcctgaaa cccggtcaaa atggaggaag ccagtcgata
     1201 tgaccggctt cctccatctc ggcgcgcagc tgtgaatcag ccggtcggct tctcgtagac
     1261 ggtgaccacc gcggcgccgc cgaggccgat gttgtgctgg agcgccttgt cggcaccttc
     1321 gacctgccgg gcatcggcgt cgccgcgcag ctgccaggtc agttccgagc actgggcgag
     1381 accggtggcg ccgagcgggt ggcccttcga gatcaggccg ccggaggggt tgaccaccgt
     1441 gttgccgccg taggtcgtgg cttcggcatc gaccagttca tggccatggc cgcgctcggc
     1501 gagaccaagt gcctcgtagg tgatcagttc gttggccgag aagcagtcat gcagctccac
     1561 cacgtccacc tcgtcgatcc cggtgcctgc ctcgttcagc gccttctcgg ccgccagccg
     1621 gctcatctcg aagccgacca ggctgatcgc gctgtcgtcg tcgaaggtct ccttggtgtc
     1681 cgtgaccagg gcctggccgg tgatctcgat cgccttgtcc cagagaccgt gctcgtcgac
     1741 gaacttctcc gaggcgacga tcgcggctcc ggacccgtcc gaagtgggag agcactggag
     1801 cttggtcagc ggcgagtaga tctccggtgc ttcctggatc tcctgaagcg tgtactcgtc
     1861 ctggaactgg gcgtaggggt tgttcaccga gtgcttgtgg ttcttgaatc cgatccaggc
     1921 aaagtgctcc ggctttgagc cgtacttctc catgtgctcg cggccggccg caccgaagat
     1981 ctgcggcgcc ggcggagcgt cctcccactg gcgcagctgg gtcagctcga tgaagtgctt
     2041 gtcgaggggc tgggtgcggt ccgtgtactt cgctcccagc gagccctttt ccattttctc
     2101 gaagcccagc gcgagggcgc agtccgcgag cccgcccttg accgactgcc gggcgaggta
     2161 gagggcggag gagccggtcg agcagttgtt gttcacgttg acgatcggta tgccgctgag
     2221 cccgaggccg tagaccgccc tttcgccgga ggtgggttcg ccgtagcagt acccgacgta
     2281 ggcgctttcg acctcgtcgt aggagatgcc ggcgtcatcg agcgccttct gtccggcctc
     2341 gaccgcccag tccgggtagt ccccttcctt ggtgccgggc ttgtcgaact tggtcatgcc
     2401 gacgccgatc acgaatgtgc ggttcatctc ttcctgctct cctcaactca gttggttgcc
     2461 ggtgctgtcg cggccgcgtc ctcggcatgg aattgtgcca agagttcgac ctcgtggtcc
     2521 ttcctgggga atgcgaagaa gaccacgagc gccccgagga tgatcgcggc gataccgacg
     2581 acataggccg aatcactgcc gttcaggaac gcggtctgcg cggcgtggac gatctgcggt
     2641 gcgtactgcg ggtgctgctc ggcgaccgtg accgccgagc cgaaggatcg ttcgagctgg
     2701 gtctgggtcg attcgctcac ctgctggctc gcctgcgggt tgccggcgat ctgggacgcg
     2761 aatgaactgg cgtaggcgga agtcaggacc gaccccagga tcgactgcat cagggctcca
     2821 ccgaggtccc gctgaaggtc ggcggtgccg gaggccatgc cggcgcggtc gaccggcacg
     2881 gagttggtca gcgaattcga cgagggggt
//
LOCUS       DAOTIP010000196         2895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40748_length_2895_cov_2.943662, whole genome shotgun sequence.
ACCESSION   DAOTIP010000196 DAOTIP010000000
VERSION     DAOTIP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40748_length_2895_cov_2.943662"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="P5138_04680"
     CDS             <1..219
                     /locus_tag="P5138_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59902.1"
                     /translation="HTARRAARGLQEVLGEIRDCDLSVPPAREMTGELEQEDVSTIVD
                     RARGNRDLDPVLVQAAPNRNAYADSSWP"
     gene            338..2428
                     /gene="ppk1"
                     /locus_tag="P5138_04685"
     CDS             338..2428
                     /gene="ppk1"
                     /locus_tag="P5138_04685"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRV59903.1"
                     /translation="METHPDPSWFFNRELSWLDFNQRVLELAEDESVPLLERVKFCAI
                     YTSNLDEFFMVRVAGLWDQVDAGIDARGPDGLSPSEQIQAIRSRSLELDRRLTGTFEE
                     KLKPELAGNGIKIATLDDLNGTERVELDRIFDQQVFATLTPLVIGLGRPFPYISNLSL
                     SIGVLLRDIETSTRVLARIKVPKELLGRFIELSSDDERVLVPLEEVIAANLDQLFPGV
                     EVLDHSYFRVTRDADFTVSDEADDLLEAVQEEVRRRRFGEVVRLEVAEGMNPELKDLL
                     VEMLRLQQNEVFEIGGLLDLTDLWNVVKLPGYPELRDQPWTPVTQPRLQTEDEDPADI
                     FAAVRQRDILVHHPYDSFSSSVERFIAQAVNDPDVLAIKQTVYRTSEDSPLVSALIKA
                     SERGKQVVCMLELKARFDEEANIEWAKRLEEVGVHVVYGIPGLKTHIKALLVVRREGD
                     HVRNYVHIGTGNYHSTTARLYTDLGLFTADPEIGADVAEMFNLLTGYSRPADYRRVLV
                     SPHSMRSSILAEIEATIAAHKAGREAKIQMKMNSLVDRTCIEALYRASQAGVEVDINV
                     RGISCLRPGVPGLSENIRVVSVVGRFLEHSRIYAFQRGEDWNVFIGSADLMPRNLDSR
                     VELVTPVEDEVARDQILDVMERCLADNTNSWVLDSSGNWSRLDPEGGERRNVQEELME
                     RHQTLASTAIPQVT"
     gene            2484..>2895
                     /locus_tag="P5138_04690"
     CDS             2484..>2895
                     /locus_tag="P5138_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRV59904.1"
                     /translation="MRFSLLPRDSTFFDLFNEAGQNTLRAAEKLNEMMAVWPDDNGLG
                     REILIIEQEGDRITHDIINRLNSTFVTPIDREDIYGLATKLDDVVDYIEEAADFLALY
                     QIEAPMDQAQDLTRILVDCCKQLSLGLENLPSFRD"
BASE COUNT          568 a          930 c          893 g          504 t
ORIGIN      
        1 catacggccc gccgtgcggc acgcggactg caggaagtgc tcggcgagat acgcgactgc
       61 gacctgtcgg tccccccggc acgggaaatg accggggaac tcgagcagga ggacgtgtcg
      121 acgatcgtcg accgggcgcg gggcaaccgt gatctggacc cggtcctggt ccaggcggcc
      181 cccaaccgca acgcttacgc ggactcgagc tggccgtgat ccatctgacc gctcggcggc
      241 agatgctcta tgaacgcttc aagcgcctct ggctggagca gtccaggcag ggcgtctggg
      301 tagcactgga aacctctctg aaagtagaat cctgaccttg gaaacacatc ccgatccatc
      361 ctggttcttc aatcgcgagc tctcgtggct cgacttcaac cagcgggtac tcgagctcgc
      421 cgaagacgaa tcggtgccac tcctggaaag ggtgaagttc tgcgcgatct acacctccaa
      481 cctggacgag ttcttcatgg tcagggtcgc gggactctgg gaccaggtcg atgccggaat
      541 cgacgctcgc gggcccgacg gactttcacc gtccgagcag atccaggcga tccgcagtcg
      601 ctcgcttgag ctggaccgtc gcctgaccgg gaccttcgaa gagaagctga agccggaact
      661 ggccgggaac gggatcaaga tcgccacgct cgacgacctc aacgggaccg aacgggtcga
      721 gctggaccgg atcttcgacc aacaggtctt cgccaccctg actcccctcg tgatcgggct
      781 gggccggccc ttcccctata tctcgaacct ctccctgagc atcggcgtgc tgctccgcga
      841 catcgagacc tcgaccaggg tcctggcccg gatcaaggtc ccgaaggagc tgctcggccg
      901 cttcatagag ctcagcagcg acgacgaaag ggtcctggtt cccctggaag aggtgatcgc
      961 tgcaaatctc gatcagctct tccccggggt cgaggtgctc gaccacagct atttccgggt
     1021 aacgcgggac gccgacttca ccgtttccga cgaggcggat gacctgctcg aggcggtgca
     1081 ggaggaagtc cggcgacgtc gtttcgggga ggtggtacgt ctcgaggtgg ccgaggggat
     1141 gaacccggaa ttgaaggacc tgctggtgga aatgctgcgc ctgcagcaga acgaggtctt
     1201 cgaaatcggc gggttgctcg acctgaccga tctctggaac gtggtgaagc tgcccggata
     1261 tccggaactt cgcgaccagc cctggactcc ggtaacccag ccccggcttc agaccgagga
     1321 tgaagacccg gcagacatat ttgccgcggt ccgacagcgc gacatcctcg tccaccaccc
     1381 gtatgactcg ttcagctcct cggtcgagcg tttcatcgcc caggcggtca acgacccgga
     1441 tgtgctggct atcaagcaga ccgtgtaccg gaccagcgaa gactcgcccc ttgtctcggc
     1501 cctgatcaag gcatccgaac gcggcaagca ggtggtctgc atgctggaac tgaaagcccg
     1561 gttcgacgag gaagcgaaca tcgaatgggc caaacgcctg gaggaagtcg gcgtccacgt
     1621 cgtttacgga atccccggac tcaagaccca catcaaggcc ctgctggtgg tccgccgcga
     1681 gggtgatcac gtccgcaatt acgtccacat cggcaccggc aactaccact cgaccacggc
     1741 ccggctctac accgacctgg ggctcttcac cgcggacccc gagatcgggg ccgacgtggc
     1801 cgagatgttc aacctgctga ccggctattc ccgtccggcc gactaccggc gggtgctcgt
     1861 ctccccgcat tcgatgcgct cgtcgatcct ggccgagatc gaggccacca ttgccgccca
     1921 caaggcgggc agggaggcga agatccagat gaagatgaac tccctggtcg accggacctg
     1981 catcgaggct ctctacaggg catcgcaggc cggggtcgag gtggatatca acgtgcgcgg
     2041 catcagctgc ctgaggcctg gcgtgccggg actctccgag aacatcaggg tcgtctcggt
     2101 tgtcggtcgc ttcctcgagc attcccgcat ctatgcgttc cagcgcgggg aggactggaa
     2161 cgtcttcatc ggatcggccg acctgatgcc gcggaacctg gactcccggg tcgagctcgt
     2221 caccccggtc gaagacgagg tcgcccgaga tcagatcctc gatgtgatgg agaggtgcct
     2281 ggccgataac accaactcct gggtgctgga cagttccggc aactggtcgc ggctggatcc
     2341 cgaaggaggc gagcgccgca acgtccagga ggagctgatg gaacggcatc agacactggc
     2401 ctccacggcc atcccccagg tgacctgatg aaaaggccat gtaaagagtt tgtgtactcc
     2461 tctgctaggt tggccacgct ctgatgcgct tctcgctctt gccacgcgac agtacgttct
     2521 tcgacctctt caacgaggcc ggccagaaca cgctgcgtgc agccgagaag ctgaacgaga
     2581 tgatggccgt ctggcccgat gacaacggtc tcggccgcga gatcctgatc atcgaacagg
     2641 agggtgaccg gatcacccac gacatcatca accggctcaa ctcgacgttc gtcacgccga
     2701 tcgaccgcga ggacatctac ggactcgcga ccaagctcga cgacgtggtg gactacatcg
     2761 aggaggccgc ggatttcctg gcgctctacc agatcgaagc accgatggat caggcccagg
     2821 atctgaccag gatcctggtc gactgctgca agcagctgtc cctcggcctc gagaacctcc
     2881 cctcgttcag ggatc
//
LOCUS       DAOTIP010000197         2893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40782_length_2893_cov_3.433051, whole genome shotgun sequence.
ACCESSION   DAOTIP010000197 DAOTIP010000000
VERSION     DAOTIP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40782_length_2893_cov_3.433051"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /gene="purH"
                     /locus_tag="P5138_04695"
     CDS             <1..560
                     /gene="purH"
                     /locus_tag="P5138_04695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578697.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRV59905.1"
                     /translation="EALEILQQKENVRIFTDEAPPFPDPKEKDIKRVRGGILIQDRDG
                     EVEQREMMEVVTRAQPSEQAWTDMLFAWTVCRHVKSNAIVFAKDGATLGIGAGQMSRV
                     DSSRLAIEKCRDAHGEEADDLLAGCAMASDAFFPFADGPMVGIEAGATSVIQPGGSKR
                     DGEVIEACDETETAMVFTRHRHFRH"
     gene            complement(581..2842)
                     /locus_tag="P5138_04700"
     CDS             complement(581..2842)
                     /locus_tag="P5138_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV59906.1"
                     /translation="MSKQKAGLDNNENGAGRAVVIGAGVAGLLAARVLSERFDRVLLI
                     ERDRLPAEAEPRKAVPQGEFPHTLLPAGLTAMETLLPDLREELGAAGAPSYSALGDVR
                     FNLLGRDLPRAALGISSVTASRPLVETTIRQLVLNRGNVDVVEGVEVSELLFDPAGKR
                     VTGVGILDRDTRLESRLGADLVAVATGRAGKLGAWLSANGYESAPEERIEVNVGYARR
                     LLRLPADSLHDKLVIVAPHADRPRGMFLFAQEDGAHTLAICGYGADRPGKDPEAYDEY
                     LASVAAPDVLAAVRAAEPLSEIQTFGYKASVRRHYEKLRRFPAGLVPIGDTLCSFNPL
                     FGQGMSVAALEAVALREVIDEHGTDPGKRWLRRAAKVLAPAWEVSSGADLALPQVEVA
                     APAPARLIGLYMQRLQRVAADDPEVAAAFMSVGALREPSSSVLRPTTLRRVLRPRDAG
                     GMALWPGEALASDVRRRRVELGGIPTKLRSAGPEHGERATVFLHGNPGSSADFEPLVA
                     AAGRLGPALAWDAPGFGESEPPANFEQSPESHAALLDASIGQLGVERVDLVMHDFGGI
                     WGLLWAAANPGRVASITLINTGVLPGYRWHRLARLWRTPVAGELFMRATSRRGFHRLI
                     QKANPKPLPGPFVDRMYADYDAVTRRAVLDLYRSVDDPAEFGREPVSALAQERIPALV
                     VWGEGDPYLPSSLAPEQRRALPGAAVHVLRGCGHWPFIDAPRKVEELLISFLAGEARA
                     PERVAMSPAPLQN"
BASE COUNT          480 a         1022 c          978 g          413 t
ORIGIN      
        1 ccgaggccct ggagatcctc cagcagaagg agaacgtccg catcttcacc gacgaggccc
       61 cgcccttccc cgaccccaag gagaaggaca tcaagcgggt ccgcggcggc atcctgatcc
      121 aggaccgcga cggcgaagtc gagcagcggg agatgatgga ggtcgtgacc agggcccagc
      181 cctcggagca ggcctggacc gacatgctct ttgcctggac cgtctgccgc cacgtcaagt
      241 cgaacgcgat cgtcttcgcc aaggacgggg ccactctcgg catcggcgcc ggccagatga
      301 gccgggtcga ctcgagccgc ctggcgatcg agaagtgccg ggacgcccat ggcgaggagg
      361 ccgacgatct cctcgccggc tgcgcgatgg cctccgacgc cttcttcccc ttcgccgacg
      421 gcccgatggt cgggatcgaa gcgggggcca cctcggtgat ccagcccggc ggctcgaagc
      481 gggacggcga agtgatcgaa gcctgcgacg agaccgaaac ggcgatggtc ttcacccgcc
      541 accgccactt ccggcactag cgtccgcggt cgggcgcgac tcagttctga agtggcgccg
      601 gtgacatggc cacgcgttcc ggcgcccgcg cctctccggc gagaaagctg atcagcagtt
      661 cctcgacctt tcgcggggcg tcgatgaacg gccagtggcc gcagccccgg agtacgtgga
      721 ccgccgcccc gggaagagcg cgccgctgtt ccggagcgag gctcgatggc aggtagggat
      781 cgccctcccc ccagaccacc agcgccggga tgcgctcctg ggccagcgcg gaaaccggct
      841 cccggccgaa ctccgcaggg tcgtcgacgc tccggtacag atcgaggacg gcccgccggg
      901 tcacggcgtc gtagtccgcg tacatacggt cgacgaacgg gccgggcagc ggcttcgggt
      961 tggccttctg gatcagccgg tggaaccccc tcctgcttgt cgcccgcatg aacaattcgc
     1021 ccgcaacggg agtccgccac agccgggcca ggcggtgcca gcggtaaccg ggaaggacgc
     1081 ccgtgttgat cagggtgatc gaagcgaccc ttccgggatt ggcggctgcc cagagcaatc
     1141 cccatatccc gccgaagtca tgcatcacga gatcgacccg ctcgaccccg agttgcccga
     1201 tgctcgcatc cagcaaggcg gcatggcttt ccggcgactg ttcgaagttg gccgggggct
     1261 cgctctcgcc gaagccggga gcgtcccagg caagcgccgg ccccaaccgt ccggccgctg
     1321 ccaccagcgg ctcgaagtct gcgctcgagc ccggattccc atgcagaaat acggttgccc
     1381 gctcgccatg ctcgggcccg gcgctccgga gcttcgtcgg aatgccaccg agctcgaccc
     1441 gcctccgccg gacgtcgctc gccagcgctt cacccggcca gagcgccatc cccccggcgt
     1501 cacggggacg gagcacccga cggagggtgg tcggccgcag gaccgacgac gacggctcgc
     1561 gcagggcacc caccgacatg aacgcggcgg caacctccgg atcgtcggca gcgacgcgct
     1621 ggagccgttg catgtacagg ccgatcaggc gggccggcgc gggcgccgcc acctctacct
     1681 gcgggagcgc gagatcggct ccggaagaaa cctcccacgc cggcgccagg accttcgccg
     1741 cgcggcgcag ccagcgcttc cccgggtcag tgccgtgttc gtcgatcacc tcgcggagcg
     1801 cgaccgcctc gagggcggcg acgctcatcc cctgcccgaa gagcggattg aagctgcaga
     1861 gggtgtcacc gatcggcacg agtccggcgg gaaatcgccg cagcttctcg tagtggcgtc
     1921 gcacgctcgc cttgtaaccg aacgtctgga tctcactgag cggctccgcc gcacgcaccg
     1981 ccgccagcac gtccggggcc gcgaccgacg cgagatattc atcgtaggcc tcgggatcct
     2041 tgccgggccg atcggcccca tagccgcaga tggcgagggt gtgcgcgcca tcctcctggg
     2101 cgaacaggaa catcccccgc gggcgatcgg catgcggggc gacaatcacg agcttgtcgt
     2161 gaagagaatc cgcgggcagt cgcaggagcc ggcgcgcgta gcccacgttc acctcgatcc
     2221 gttcctcggg cgcggactcg tagccgttgg cggaaagcca tgccccgagt ttgccggcac
     2281 gaccagtggc aacggcgacg agatcggcgc cgagcctcga ctccaggcgg gtgtcacggt
     2341 caaggatgcc gacgccggtc acccgcttgc cggcgggatc gaagagcagt tcgctgacct
     2401 caacaccctc gaccacatcg acgttcccgc gattcaggac gagctggcgg atggtcgtct
     2461 ccacaagtgg ccggctggcg gtcaccgagc taatgccgag ggccgcccgg ggaagatcac
     2521 gacccagcag gttgaagcgg acatctccga gcgccgaata gctcggagcg cccgccgcac
     2581 cgagttcctc cctgaggtcg ggaaggagtg tctccatggc ggtgagcccg gcgggaagca
     2641 gggtatgagg gaactcgccc tgcggcacgg ccttgcgggg ttcggcttcg gcgggaaggc
     2701 ggtcgcgttc gatgagcagg acgcggtcga accgttcgct cagcacccgc gcagccagca
     2761 atccggccac tccggcgccg atcacgacgg cacggcctgc gccattctcg ttgttgtcca
     2821 gtccagcttt ctgcttgctc atcgtggcct cctggcgacc ttcgtttggc tgatgagtgc
     2881 gaagctaggc gca
//
LOCUS       DAOTIP010000198         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_40791_length_2892_cov_4.379979, whole genome shotgun sequence.
ACCESSION   DAOTIP010000198 DAOTIP010000000
VERSION     DAOTIP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40791_length_2892_cov_4.379979"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="P5138_04705"
     CDS             complement(<1..432)
                     /locus_tag="P5138_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59907.1"
                     /translation="MSHNDEKDFGLDREFADLALALREARPTPEPGFTERLDQAVADH
                     FPPEWASDAALGKKNLGGIGSLAERFRRRLGGRRVLLPAMAGFAGLLLVATVVVSAVD
                     SGRLGFGGDKSETATTTSSDNPVPLSLDQSSAPESSAGGLGM"
     gene            complement(429..1079)
                     /locus_tag="P5138_04710"
     CDS             complement(429..1079)
                     /locus_tag="P5138_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV59908.1"
                     /translation="MLEAVPQPDSSPPGTGSRPKSGPRPLFEDLYRGSRDDLYAYVAG
                     LLRDRVLAEDVTALAFERAFRKWDRYDPKRGSARGWLFGIARNAALDELRKRKREVPA
                     IEPGGLEAPGSVAGANRSGDPAPVESRIVQLEQRAELLGAMGTLAPADREIIALKFFA
                     GLNNTEIAEATGYSPSNVGTRVGRAMKRLRSALTDGSAEGGSEATDDPAPDEEVAR"
     gene            complement(1303..1482)
                     /locus_tag="P5138_04715"
     CDS             complement(1303..1482)
                     /locus_tag="P5138_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRV59909.1"
                     /translation="MSTATSQLWICESCGWIYDPAEGDPDGGIPAGTAFEDIPEDWFC
                     PVCGARKTDFEPYEG"
     gene            1586..2230
                     /locus_tag="P5138_04720"
     CDS             1586..2230
                     /locus_tag="P5138_04720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRV59910.1"
                     /translation="MNSFPEAGPGEAINLGRPAEPRPAATIVLLRDRQEGGPLEVLML
                     KRSAESHFMPNVWVFPGGSLDPEDGEGLDGLRTCAARELEEEAGIRIDLSTELIPLAR
                     WVTPEVVKTRFDTWFFLARAPEGVVVKPDNFEMTEALWVTPTDALAASEAGDMAIVFP
                     TLKQLEALVPAAHTEEALRHAAADPNASKVVLPKVIGDEKNHRVVLPGDDDYPD"
     gene            complement(2237..>2892)
                     /locus_tag="P5138_04725"
     CDS             complement(2237..>2892)
                     /locus_tag="P5138_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59911.1"
                     /translation="WQQIVSSYQGAGEVKWESVGGINPVDGPVALCVGGSNRLTGQLA
                     PDFWGASCDADEEEVGGFMRRSVVPTAILAKAHMPDLAKVVSAFNVESIEANAGEFVK
                     QKVSRRKVEFESLEFNQRFLATVPEDHDPVALRELFSPGFLSWTTTIDREVDFGINDR
                     QFWFLWRLRERSAEELKLALANAGELFRRLHSEMEEEGIHIYPAGPWNAGLAPFPAD"
BASE COUNT          487 a          963 c          960 g          482 t
ORIGIN      
        1 catgccaagt ccgcctgccg aggactcggg ggcagaagac tggtcgagcg aaagcgggac
       61 cggattatcg gaggacgtgg tcgtagccgt ttccgatttg tcgccgccga atcccagacg
      121 tccggaatcg accgcgctga ccaccactgt ggcgaccagc agcaggcccg cgaagccggc
      181 cattgccggg agcaggacgc ggcggccgcc gagacggcgg cggaagcgct cggccaggct
      241 gccaatgcca cccaggttct tcttgcccag agccgcgtca cttgcccact ccggtgggaa
      301 gtggtcggcc accgcctggt cgagtcgttc ggtgaagccg ggttccgggg tcggccgggc
      361 ctcgcggagg gcgagggcca ggtccgcgaa ctcgcggtcg agtccgaagt ctttttcgtc
      421 gttgtggctc atcgggccac ctcctcgtct ggcgctgggt catctgttgc ttcggatccc
      481 ccctcagcgg agccgtctgt gagtgcgctc cgaagtcgtt tcattgcgcg ccccacgcgg
      541 gtgcccacgt tgctgggcga gtagccggtc gcctcggcga tctcggtgtt gttgaggccg
      601 gcgaagaact tgagggcgat gatctcccgg tcggccggag cgagggttcc catcgccccg
      661 agcagttcgg ccctttgctc gagctggact atgcggctct cgaccggagc cggatcgccg
      721 gatctgtttg cgccggccac cgagcccggg gcctccagcc ctccgggctc gatcgcgggc
      781 acttcccgct tgcgctttcg aagctcgtcc agggcagcgt tccgggcgat gccgaaaagc
      841 cagccgcggg cactgcctcg cttcgggtcg taacggtccc atttgcgaaa ggcccgctca
      901 aaggcgagcg cggtcacgtc ttcagccagg acgcggtcgc gcagcagacc ggccacatag
      961 gcgtaaaggt cgtcccggct gccgcgatag agatcctcga aaagaggccg cggcccactc
     1021 ttcggacggg atccggtacc gggcggggac gagtcaggtt gagggaccgc ttcaagcatg
     1081 ccgttcatac cgacactacg gcccgattgg caatccgtca cacccgactt ccagcgggtg
     1141 gaacaaaggc ttcagcgggt cgtgcacagg ggcctccagc cgtgcgctgc cgaggaggca
     1201 agcgaaggcg gtgaaggggg cgtactggaa gtacgttccc gaaccgtcga gcgaccgacg
     1261 aaggtcagcg tgcggctggt ggttcctggg cgcgttgccc gatcagcctt cgtagggctc
     1321 gaaatctgtc ttgcgcgccc cgcaaaccgg gcagaaccag tcttcgggaa tgtcttcgaa
     1381 tgcggtgccg gcgggaatgc cgccgtccgg gtcgccctcg gccggatcgt agatccatcc
     1441 acaggattcg cagatccaga gctgggaagt tgcggtactc atggcggcca gattacccga
     1501 ggcgagccct gcgaaacctc ccggtcagtc cggatccgga ctggcgcctg ggcggaaacc
     1561 acggcttggc gatacggttc caaccgtgaa ctcgtttccg gaagccggac ccggggaggc
     1621 gatcaatctc ggcaggcccg ccgagccgcg tcccgccgcc acgatcgtcc tgctgcgcga
     1681 ccggcaggaa ggcggcccgc tcgaggtgct gatgctgaag cgctcggccg agtcgcattt
     1741 catgccgaac gtctgggtct tccccggtgg ctcccttgat cccgaagacg gcgaaggcct
     1801 cgacggactg cgcacctgcg ccgcccgaga gctcgaggag gaggccggga tcaggatcga
     1861 cctttcgacc gagctgatcc cgctggcccg ctgggtcacc ccggaagtcg tgaagacgcg
     1921 gtttgacacc tggttcttcc tcgcccgggc cccggaaggc gtggttgtca aacccgacaa
     1981 cttcgagatg accgaggcac tctgggtgac cccgaccgac gccctggccg catccgaggc
     2041 aggggacatg gcgatcgtct tccccaccct gaagcaactc gaggcactgg ttccagcggc
     2101 ccacaccgag gaggccctac gccacgccgc ggccgacccg aacgcctcga aggtcgtcct
     2161 gccgaaggtg atcggagacg aaaagaacca cagagtggtc ctccccggcg acgacgacta
     2221 tccggactga gcccggtcag tcggccggga agggagcaag ccccgcgttc cagggcccgg
     2281 ccggatagat gtggatccct tcctcttcca tctcggagtg gagccggcgg aacagttcac
     2341 cggcgttggc cagggccagt ttcagttcct cagccgacct ttccctcagg cgccagagaa
     2401 accagaactg ccggtcgttg atcccgaaat ccacctcgcg gtcgatcgtc gtcgtccacg
     2461 agagaaaacc cggtgagaac agttcccgca gggcgaccgg gtcgtggtcc tcgggcacgg
     2521 tggcaaggaa acgctgattg aactcaagcg attcaaactc gaccttccgg cgagagacct
     2581 tctgcttgac gaactcgccc gcgttggctt cgatcgactc gacgttgaag gcggacacga
     2641 ccttggccag gtccggcatg tgggccttgg cgaggatcgc ggtcgggacg acgctcctcc
     2701 gcatgaatcc gccgacctcc tcttcgtcgg cgtcgcagct ggctccccag aagtcggggg
     2761 cgagctggcc ggtcagccgg ttgctgccgc ccacgcacag tgcgaccggg ccgtcaaccg
     2821 ggtttatccc cccgactgac tcccatttga cctctcccgc accctggtag gaggagacga
     2881 tctgctgcca ct
//
LOCUS       DAOTIP010000199         2876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41118_length_2876_cov_3.825594, whole genome shotgun sequence.
ACCESSION   DAOTIP010000199 DAOTIP010000000
VERSION     DAOTIP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41118_length_2876_cov_3.825594"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="P5138_04730"
     CDS             <1..274
                     /locus_tag="P5138_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59912.1"
                     /translation="LTADQRSEFAHQLTGGVPHLEGVSAAKADRLVAAGHDAFIYGLT
                     HAMTLSVGVAVVGVLVAFLLISGKAESTDEQTQEAAEIAEAASPVA"
     gene            329..1447
                     /locus_tag="P5138_04735"
     CDS             329..1447
                     /locus_tag="P5138_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRV59913.1"
                     /translation="MHVRTLTNFADRPRFFALALACAAVALFSLAGSAHAANGGATPS
                     GPAKATVKIKLKGIHARQATVMSKVSILGSLKPFRNLQTVKVYLYRDGKRISTRKVKV
                     HRAKGNYGTFRTSIYMRGGTRYGVHAKYWGKAGKRAVSKDATEHKVWRVRYVGLAHDQ
                     CGRVVAGFRKALNRLALVPAQGRCFNGKMERAVLAYRKLNDMTRNSSASRTIVKRVFN
                     GNGGYHVRRPELGDHMEAPLSKQVIVFSKGKRPYAIFPIASGKASTPTILGTYSFYWK
                     EPGYNSHGMYYSSYFIRGYATHGYASVPNYPASHGCLRTFISDQPRIYNITEIGMPVY
                     TFGNAFRGRVAPYRVKRSGGDLGPDLGATGGLDPTSKY"
     gene            1491..2174
                     /locus_tag="P5138_04740"
     CDS             1491..2174
                     /locus_tag="P5138_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRV59914.1"
                     /translation="MTVAAAGVLMAGGQAGGSQSGSAQPEARAKAGKVPAWIHQRFIP
                     FGAKRKHQMARYSLRHYGVRAWRLRHPRQIVEHVAVAGTIAQVIHSFVPDRPDPEFHE
                     LPNVCAHFVVSGRGRVVQLVPVGIRCRHVVGLNHVAIGIEHTGYRDGDVLGNRRQMRA
                     SIRLTRWLRCRYRIGIRDVIGHRESLSSRFYRELVPAMKGQTHGDFRHTSMRRYRKAL
                     FNAGRCPRT"
     gene            2280..>2876
                     /locus_tag="P5138_04745"
     CDS             2280..>2876
                     /locus_tag="P5138_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRV59915.1"
                     /translation="MSNIAMTSLELARWQFGITTVYHFIFVPLTIGLAFFVAWYQTRW
                     YRTKEEKWLRATRFWGKLMLISFALGVATGIVQEFQFGMNWSQYSRFVGDIFGAPLAM
                     EGLAAFFVESTFLGLWIFGWGRLSEKVHLICMWLVSLSTMLSAFFILAANSWMQHPVG
                     YELNKATGKVELTSIWDVLTNSTALYAMTHTMLAAALTG"
BASE COUNT          504 a          975 c          881 g          516 t
ORIGIN      
        1 cctgaccgcc gaccagcgct ccgaattcgc ccatcagctg accggcggtg taccccacct
       61 cgaaggtgtg tccgccgcca aggccgatcg cctggtcgcg gccggacatg acgccttcat
      121 ctatgggctg acgcatgcca tgaccctctc ggtaggggtc gccgttgtcg gtgtcctggt
      181 cgctttcctg ctgatcagcg gcaaggccga gtcgacggac gagcagacgc aggaggcagc
      241 agagattgcc gaagccgcct caccagtggc ctgatccgtc tgtatctcta caatttgccg
      301 acttgtcgcc caatcgcagg agcttcgctt gcacgtccga acgctgacca atttcgctga
      361 ccgaccccgc ttcttcgcgc tggcccttgc ctgcgcggcg gtggcactct tctcgctggc
      421 cggcagcgct catgcggcca acggcggcgc cactccgagc ggaccggcca aggcgacggt
      481 gaagatcaag ctcaagggca tccatgcccg ccaggcgacg gtgatgagca aggtcagcat
      541 cctcggctcg ctgaagcctt ttcgcaacct gcagacggtc aaggtctacc tgtacaggga
      601 cggcaagcgc atctccaccc gcaaggtcaa ggtccacagg gccaagggca attacggcac
      661 cttccgcacc agcatttaca tgcgcggcgg aacccggtac ggcgtccacg ccaagtactg
      721 gggcaaggct ggcaagcggg cggtcagcaa ggacgccacc gaacacaagg tctggcgggt
      781 ccgttacgtg ggcctcgcgc atgaccaatg cggccgtgtg gtcgccggct tccgcaaggc
      841 cctcaatcga ctcgcgctgg ttcccgccca gggtcgctgc ttcaacggaa agatggaacg
      901 agccgtcctc gcctaccgca agctgaacga catgacccgc aactcgagcg cctcgagaac
      961 catcgtcaag cgggtcttca acggcaacgg cggttaccac gtgcggcgcc ccgagctcgg
     1021 cgaccacatg gaagcaccgc tttccaagca ggtgatcgtc ttctcgaagg gcaagcgccc
     1081 ctacgcgatc ttcccgatcg ccagcggcaa ggcttcaacc ccgaccatcc tcggcactta
     1141 cagcttctac tggaaggagc cgggctacaa ctcgcacggg atgtactact cgtcgtactt
     1201 catccgggga tacgccacgc acggctacgc ctcggtgccg aactacccgg ccagccacgg
     1261 ctgcctcagg accttcatct cggaccagcc ccggatctac aacatcaccg agatcgggat
     1321 gccggtctac accttcggca acgccttccg cggacgggtc gccccctaca gggtgaagcg
     1381 ctcgggcggc gacctcggac cggatctcgg ggcgaccggc ggcctggatc cgaccagcaa
     1441 gtactgagcc gatctcacgg cggggtctca acagattcgc cgcggccgtc ttgacggtcg
     1501 cggcagccgg tgtcctgatg gccggtggtc aggcgggcgg atcgcagtcg ggatccgctc
     1561 agccggaggc ccgggcgaag gccgggaagg tgccggcctg gattcaccag cgcttcatcc
     1621 cgttcggggc gaagcgcaag caccagatgg cccgttactc gctccgccac tacggagtgc
     1681 gggcctggcg gctcaggcat ccccgacaga tcgtggaaca cgtggcggtg gccggaacga
     1741 tcgcccaggt cattcattcc ttcgtgcccg accgtcccga ccctgagttc cacgaactgc
     1801 ccaacgtctg tgcgcatttc gtggtttccg gccggggcag ggtggtccag ctcgtcccgg
     1861 tggggatccg ctgccgtcac gtggtcggcc tgaaccacgt tgcgatcggg atcgagcaca
     1921 cgggctaccg ggacggcgac gtgctcggca accgtcggca gatgcgagcc tcgatccggc
     1981 tgaccaggtg gctgcgctgc cgctaccgga tcggcatccg tgacgtgatc ggccaccggg
     2041 aaagcctgag ctcccgcttc taccgggaac ttgtcccggc gatgaagggc cagacccacg
     2101 gtgatttccg tcacacatcg atgcggcgtt accgcaaggc cctgttcaac gcaggccgtt
     2161 gcccgcggac ttagctcagg ctaagtcggg gcgtaggtat ttcaccgcaa ccgttgcggt
     2221 gattttccgc aggatttcgt cccgccacat ggatactctg cccgcatgaa ggtggaacca
     2281 tgagcaacat tgcgatgacc agtctcgagt tggcccgctg gcaattcggg atcaccacgg
     2341 tctaccactt catcttcgtg ccgctcacta tcgggctggc attcttcgtc gcctggtacc
     2401 agacccgctg gtaccggacc aaggaagaga agtggctgag ggcaacccgc ttctggggca
     2461 agctgatgct catctccttc gccctcggtg tggcgacggg catcgtgcag gagttccagt
     2521 tcggaatgaa ctggtcccag tactcccgct tcgtcggcga catcttcggc gccccgctgg
     2581 cgatggaggg cctcgccgcg tttttcgtcg aatcgacctt cctcggcctc tggatattcg
     2641 gctggggccg gctttcggag aaggtccacc tgatctgcat gtggctggtt tccctttcga
     2701 ccatgctctc ggccttcttc atcctggccg ccaactcctg gatgcagcac ccggtcggct
     2761 acgaactgaa caaggccacc ggcaaggtcg agctcacctc gatctgggac gtgctgacca
     2821 acagcaccgc gctgtacgcg atgacccaca cgatgctggc cgcggccttg accggc
//
LOCUS       DAOTIP010000200         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41167_length_2874_cov_2.369280, whole genome shotgun sequence.
ACCESSION   DAOTIP010000200 DAOTIP010000000
VERSION     DAOTIP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41167_length_2874_cov_2.369280"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="P5138_04750"
     CDS             complement(<1..387)
                     /locus_tag="P5138_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV59916.1"
                     /translation="MSERDGNMTDGRVTLQDVASLAGVSQATAGRALGGYGSISEDSR
                     QRVEQAATKLGYRTNAVARALASGTTNGAPQASQWRKHVTPDARIVGAAKSFLHRDHL
                     ASVRITTNASGAQTRRVTYKAFGEQAA"
     gene            complement(384..1892)
                     /locus_tag="P5138_04755"
     CDS             complement(384..1892)
                     /locus_tag="P5138_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV59917.1"
                     /translation="MTGQPEAMRFDIDWHRRAEQIELEGRAFIDGSHADAISGGQMET
                     VKPRDGSVLARIAACDAADVDAAVSAARRSFESGQWSEAHPRVRKRVLHELVDRIQAD
                     REEIGLLISLEMGKPITDAIGEVDSSCRELAFFAEAVDKVYGNTVPTDAKSLGMSVRE
                     PKGVIGAVTPWNFPLMMPIYKVAPALAAGNSIVLKPAEQSSLAAIRLAELASEAGLPD
                     GVLNVVPGTGEKAGRSLGLHMDVDAITFTGSTSIGKAFLAYSSESNMKGVWLECGGKS
                     PNVVLADAPDLDRAAEFAAETIFHGAGQVCNAGSRLIVERPIYEDFVSRVARESLKWA
                     PADPLDASAKMGPLVDRPALDRALGYVSAAKSDGATVVSGGGETLEATGGYFMEPTIL
                     GSVDNSMPVAQEEIFGPVLAAIELEERGEATRLANGSRYGLAAAIWTSDFGRAHRVAR
                     ALKAGTVYINCYDRGDNSLPFGGFKESGIGVDRSLAAMEKYSNLKTIWADIS"
     gene            complement(1893..>2481)
                     /locus_tag="P5138_04760"
     CDS             complement(1893..>2481)
                     /locus_tag="P5138_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRV59918.1"
                     /translation="RSKWPEDGFLGEESGSSDGTSARVWCVDPLDGTANFAAGYPRWA
                     VAIALLIRGEAVLAVTFDPLREEIFVAREDVGLTSGPGLSNMRAVARIEDALVADNRP
                     YDHREPAQPLEMEFRRSRGRRESGSMALDFAWLAAGRLDVVSYERSERLWDYIGGELL
                     VAQAGGEIRIADPARDIAVAASPELVEYLCPTKDE"
     assembly_gap    2482..2491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2656..>2874)
                     /locus_tag="P5138_04765"
     CDS             complement(2656..>2874)
                     /locus_tag="P5138_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59919.1"
                     /translation="KESIPVYTPDRWLVGQYELAIRFAGAEIARRITGVGKANQIDAL
                     PEARMLDASRKLLGEARQLISSGRPAWI"
BASE COUNT          473 a          979 c          894 g          518 t
ORIGIN      
        1 cgccgcctgc tcgccgaagg ctttgtacgt gacgcgccgc gtctgcgcgc cgctcgcatt
       61 cgtcgtgatc cgcaccgagg cgaggtggtc gcgatgcagg aagcttttcg ccgcgccgac
      121 gatccgcgcg tcgggcgtca catgcttgcg ccattgcgaa gcctgcggcg cgccattggt
      181 cgtgcccgag gcgagcgccc gggcgacggc gttggttcgg taccccagct tggtcgccgc
      241 ctgttcgacg cgctggcggg aatcctcgct gatcgacccg tagccgccca gtgcccggcc
      301 ggccgtagcc tgggaaacac cggcgagcga ggccacatcc tgaagagtca cccgaccgtc
      361 ggtcatgttt ccgtctcttt cgctcatgaa atgtcggccc agatcgtctt caggttcgag
      421 tatttctcca tcgcggcgag cgaccggtca acgccgatcc ccgactcctt gaaacccccg
      481 aagggaagtg aattatcgcc gcggtcgtag cagttgatgt aaacggttcc cgccttgagg
      541 gcgcgcgcga cgcggtgggc ccggccaaag tccgaggtcc agatcgcggc ggcgagtccg
      601 tatctcgagc cattggcgag tcgcgtggcc tcgccccgtt cttccagctc gatcgcggcg
      661 agcaccgggc cgaagatctc ctcttgcgca accggcatcg agttgtcgac cgagccgaga
      721 atcgtcggtt ccatgaagta cccccctgtg gcctcgaggg tctcgcctcc gccgctgacc
      781 acggtcgccc cgtcggactt tgcggcggac acgtaaccga gagcccggtc gagggccggc
      841 cggtcgacca gtgggcccat cttcgccgag gcgtcgagcg ggtctgccgg cgcccacttg
      901 aggctttccc gggcaactcg acttacgaag tcttcataga tcggtcgctc gactatgagt
      961 cgggatcccg cgttgcagac ctgaccggca ccgtggaaga tcgtctccgc ggcaaactct
     1021 gcggcccggt cgaggtcggg cgcatcggcc agcaccacgt tgggagactt cccgccgcat
     1081 tccagccaga cgcctttcat gttcgactcg gatgaatagg cgaggaaggc cttgccgatc
     1141 gaagtcgaac cggtgaacgt gatcgcgtcg acgtccatgt ggaggcccag cgaccgcccg
     1201 gccttctcgc cggttcccgg cacgacgttg aggacgccgt ccggaagacc ggcttcggag
     1261 gcaagctcgg ccagcctgat cgccgccagc gaagattgct cggccggttt cagcacgatc
     1321 gagtttccag ccgcgagcgc gggagcgacc ttgtagatcg gcatcatcag ggggaagttc
     1381 cagggcgtga cggccccgat cactcccttc ggctcgcgca ccgacatgcc gagtgatttc
     1441 gcatcggtgg gaaccgtatt tccgtaaacc ttgtccaccg cttcggcgaa gaaggcgagc
     1501 tcgcgacagg aggaatcaac ttcaccgatc gcgtcggtga tcggcttgcc catttccagc
     1561 gagatcaaca gaccgatctc ttcccggtcg gcctggatcc gatcgaccag ttcgtggagc
     1621 acgcgcttgc gtacccgggg atgagcttcg gaccactggc ccgactcgaa cgacctgcgc
     1681 gccgcgctga ccgccgcatc cacgtcggcc gcgtcgcatg cggcaatcct ggcgagcaca
     1741 gacccgtctc tcggtttgac ggtctccatc tgcccgcccg aaatcgcatc ggcgtgcgag
     1801 ccgtcgatga aggcgcgccc ctcgagctcg atctgctccg ccctcctgtg ccaatcgatg
     1861 tcgaatctca ttgcttcagg ctgtccagtc atctactcat ccttcgtcgg gcacaggtat
     1921 tcgacaagtt cggggctggc agcgacggcg atgtcacgag ccgggtcggc gattcgtatt
     1981 tcgcctccgg cctgggctac gaggagctcg ccgccgatgt agtcccagag ccgctccgag
     2041 cgctcgtaac tgaccacgtc aagccggcct gccgcgagcc aggcaaagtc gagagccatc
     2101 gaaccggact cgcgccggcc ccgcgaccga cggaactcca tctccagggg ctgggccggc
     2161 tcgcggtgat cgtacggccg gttgtccgcg accagggcat cctcgattcg ggcgaccgca
     2221 cgcatattcg aaagacccgg gcctgaagtc agaccgacat cttcccgggc gacgaagatc
     2281 tcctctcgca gaggatcgaa cgtgacggcc agcactgcct cgcctcgaat caagagcgcg
     2341 atcgcaacgg cccaacgggg atagccggca gcgaagttgg cggttccgtc gagtggatcg
     2401 acgcaccaca cccgggccga tgtgccgtct gagcttccgc tctcttcgcc caggaagcca
     2461 tcctctggcc acttgctcct cnnnnnnnnn ncctcaggtg gccgatgatc gcctgttcgg
     2521 cgtcgaggtc cgcctgggtt accacgtcct gacgcactgc tttgctccgg gtttccacga
     2581 ccccgttctc gaaacaccgg cgaagcatcg gaccgaccat gacggccgct tcggtcgccg
     2641 cggcagcttc cagttctaga tccatgcggg cctcccggac gagatcaact ggcgcgcttc
     2701 gccgagcagc ttgcggctgg catcgagcat ccgggcctca ggcagggcat cgatctgatt
     2761 cgccttgccg accccggtga tccttcgggc gatctcggcc ccggcgaagc gaatggccaa
     2821 ttcgtactgg ccgacgagcc agcgatcggg ggtgtaaacc ggaatagact cttt
//
LOCUS       DAOTIP010000201         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41180_length_2873_cov_3.359475, whole genome shotgun sequence.
ACCESSION   DAOTIP010000201 DAOTIP010000000
VERSION     DAOTIP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41180_length_2873_cov_3.359475"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="P5138_04770"
     CDS             complement(<1..602)
                     /locus_tag="P5138_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV59920.1"
                     /translation="MEPTAATESISVMNPATGEQVGSVPVDSAGDVAAAVARIRGNQP
                     AWEALGFKGRRKWLNELRDWLLDNSDRIADTLKAETGKVRAEATIETPYLADQINFYG
                     SKAEKFLGEQKVRPNTMLAATRKLRLQYRPYPVVGLISPWNFPLVLSLGDAIPALMAG
                     AAVVLKPSEITPLGLNEIVDAWRNEIGAPDVITVVNGGAET"
     gene            complement(671..1888)
                     /locus_tag="P5138_04775"
     CDS             complement(671..1888)
                     /locus_tag="P5138_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRV59921.1"
                     /translation="MSGARRRRWAPAVACMAGLAILFAAACPSAGAAPKLGYVKVASA
                     RQPTSLGSPPGYPRMLFVAERAGKIRILRRGRWLKNPLLSLNRKVSSRNIERGLLGLA
                     FPPDFRKTGRFYVDYTARNGNSVVAEYRTGRGDPTRLRPGSGRVILEIPRVNGNGNHN
                     GGHLAFRGNLLYIAVGDGFDPGDAPDNAQNLESLRGKILRIDPRPDPGAGTAYRIPPG
                     NPFVGRPGRDEIFAYGLRNPFTFSFFDSGGQAMMSIADVGQLRYEELNVLPFTEARGA
                     NFGWKGFEGFEPFNCGELCPNGADPLVTTGLEWPQLVYSHEDGCAIIGGLPINDPKLG
                     AFRGRVIYGDFCTGRVRTAAPRLPTIVDDRPAGFSLPSRHGVALLNGFGTDGLKRLYA
                     FSHYGGIYRIIRR"
     gene            complement(1885..>2873)
                     /locus_tag="P5138_04780"
     CDS             complement(1885..>2873)
                     /locus_tag="P5138_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRV59922.1"
                     /translation="FPNLMFVVEQPGRIMVLRRGRKLARPFLDIRSRVDFDGSERGLL
                     SVAFPPDYRKTKRFYVYFVDNAGNIRVVEFRRKSAVRARQNSARLVIRIAHPVNANHN
                     GGRLGFLGNNLFFGTGDGGGSGDQPGNAQNLNSLLGKLIRIDPRKSGRRAYTVPASNP
                     FVGRPGRDEIFAYGLRNPFRWSFDRTSSKQKVRIAIGDVGQDEWEEINYLNLSRARGG
                     NFGWNRWEGFTPFDGGGAGTIKPSLVLPHPPNCSVIGGLVVRDRSLPALRGRYVFTDF
                     CNGRILSFKPHLGRAAGRPTGLNIDNVTSFGESLKGTLFITSLDGSVYRIRQ"
BASE COUNT          455 a          907 c         1010 g          501 t
ORIGIN      
        1 gtctcggctc cgccgttcac caccgtgatc acgtccgggg cgccgatctc gtttcgccag
       61 gcgtcgacga tctcgttcag tccgaggggg gtgatctcgg aaggcttcag gaccaccgcg
      121 gcgccggcca tcagggccgg gatcgcgtct ccgagagaga ggacgagggg gaagttccag
      181 gggctgatca ggccgaccac cgggtagggg cggtactgca gccgcagctt ccttgtcgcg
      241 gcgagcatgg tgttggggcg taccttctgt tcgccgagga acttctcggc cttcgacccg
      301 tagaagttga tctggtcggc caggtaggga gtctcgatcg tggcttcggc ccggaccttt
      361 ccggtctcgg ccttgagcgt gtcggcgatc cggtccgaat tgtcgagcag ccagtcgcga
      421 agctcgttca gccacttccg ccggcccttg aagccgagcg cctcccaggc gggctggttg
      481 ccgcggatcc tggctaccgc agcggcgacg tcaccggcgg agtcgaccgg gaccgacccg
      541 acctgctctc cggtggcggg gttcatcacg gaaattgact cggttgctgc ggtcggttcc
      601 acttactgct ccagtctctc tcgtcgctct tcacctgagc gaaagcccac ccaggcgttc
      661 aatacaaagg ttagcgccga atgatccggt agatgccgcc gtagtggctg aatgcgtaaa
      721 ggcgcttcag gccatcggtc ccgaaaccgt tgagcagggc gacgccgtgc ctggacggca
      781 gggagaaacc ggcgggccgg tcgtcgacga tcgtgggtag gcggggggcg gcggtccgta
      841 cccggccggt gcagaaatcg ccgtagatga ccctgccccg gaaggccccc agcttcgggt
      901 cattgatcgg gaggcccccg atgatcgcgc agccgtcctc atgcgagtag accagctgcg
      961 gccactccag gccggtggtg acgagcgggt cggcgccgtt ggggcagagc tcgccgcagt
     1021 tgaacggttc gaatccctcg aaacccttcc atccgaaatt ggcgccccgg gcttcggtga
     1081 aggggagaac gttgagctcc tcgtatcgga gctggccgac gtcggcgatc gacatcatcg
     1141 cctggccgcc agagtcgaag aagctgaagg tgaacgggtt gcgcagcccg taggcgaaaa
     1201 tctcgtcccg gccgggccgg cccacgaagg gattgccggg aggtatccgg taggccgttc
     1261 cggccccggg gtccggcctt gggtcgatcc gcaggatctt tccgcgcagg ctctccaggt
     1321 tctgggcgtt gtcgggagcg tcgccgggat cgaagccgtc gccgaccgct atgtagagga
     1381 ggtttccccg gaacgcgagg tgcccgccgt tgtggttgcc gttcccgttg accctcggga
     1441 tctccaggat cacccggccg ctgcccggtc tcagccgggt cggatcaccg cgcccggtcc
     1501 ggtattcggc gaccactgag ttgccgttcc tggcggtgta atcgacatag aaccgaccgg
     1561 ttttccggaa gtcggggggg aaggcgaggc cgagcagccc gcgttcgatg ttgcgggaac
     1621 tcaccttgcg gttcaggctg agcaggggat tcttcagcca cctgcctcgt cggaggatcc
     1681 ggatcttgcc ggccctttcg gccacgaaga gcattcgcgg gtaaccggga ggtgagccca
     1741 ggctggtcgg ctgccgggcg ctcgccactt tcacatagcc cagcttcggg gcggcgccgg
     1801 cggaagggca ggctgccgcg aaaaggattg ccaggccggc catgcaggcg acggccgggg
     1861 cccaccggcg gcggcgggcc ccgctcattg ccggatccgg tagacgctgc cgtcgagtga
     1921 ggtgatgaac agggtgccct tcaggctttc gccgaaggag gtgacgttgt ctatgttcag
     1981 gccggtcgga cgtcccgcgg cgcgtcccag atgcggcttg aagctcagga tcctgccgtt
     2041 gcagaagtcc gtgaacacat aacggccgcg cagggccggc aggctgcggt ccctgacgac
     2101 gaggccgccg atcaccgagc agttcggagg atgcgggagg acgagcgacg gtttgatcgt
     2161 tcccgccccg ccaccgtcaa agggcgtaaa cccttcccag cggttccagc cgaagttgcc
     2221 cccgcgggcg cggctcaggt tcaggtagtt gatctcctcc cattcgtcct gcccgacgtc
     2281 gccgatcgcg atccgcacct tctgcttcga actcgtccgg tcgaaggacc agcggaaagg
     2341 gttgcgcaag ccgtaggcga agatctcgtc ccggccgggc cggcccacga agggattcga
     2401 ggccggcacg gtgtaggcgc gacggccact cttgcgcggg tcgatccgga tcagcttgcc
     2461 gagcagcgag ttcaggttct gggcgttgcc cggctggtcg ccggatccgc ccccgtcgcc
     2521 ggtgccgaag aagaggttgt tgccgaggaa acccagccgg ccgccgttgt gattggcgtt
     2581 gaccggatga gcgatccgga tcaccagccg ggccgagttc tgccgggccc ggacggcgct
     2641 cttgcggcgg aactcgacca cccggatgtt tccggcgttg tcgacgaagt acacgtagaa
     2701 gcgtttggtc ttccggtagt cgggcgggaa ggcgaccgag agcaggccgc gttcggagcc
     2761 gtcgaaatcc acacgcgagc ggatgtcgag gaagggccgg gccagtttcc ggccccggcg
     2821 gagaaccatg atccgtcctg gctgttcgac cacgaacatc aggttcggga agc
//
LOCUS       DAOTIP010000202         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41186_length_2873_cov_3.038680, whole genome shotgun sequence.
ACCESSION   DAOTIP010000202 DAOTIP010000000
VERSION     DAOTIP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41186_length_2873_cov_3.038680"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="P5138_04785"
     CDS             complement(<1..744)
                     /locus_tag="P5138_04785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV59923.1"
                     /translation="MRIALVTPYSWTYQGGVNRHVQSLAEEYISRGHYVRVIAPFDPP
                     DRLSKVLHRAEAEDREIPDYLIPVGRTFGINANGAVSNIPLFPSGVVETRRAIEEGDF
                     DVVHLHEPTTPANGWDTCLSSDVPVVGTFHAYSTKPLPNYIANAVGARRTLNRLSARI
                     AVSQAAAWTGRRWFGGNYTIVPNGVDIDAAPTGPKPETDHLRALFVGRPDERKGLPVL
                     LRAFSALVEHAPARLTVVGADPDDVHRILA"
     gene            complement(787..2637)
                     /locus_tag="P5138_04790"
     CDS             complement(787..2637)
                     /locus_tag="P5138_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59924.1"
                     /translation="MSDAATGHEGQVTIVVLPQGTSPADLAGLEHAAIGLMNPGLGDV
                     SAQQTWLDVSQGARAFDSTYDTPLDRVYPGGEFAQGWEKVVRRAKSNDTTLIPGLMAS
                     VLAGHDLNAVAADSAGAAALAVARRDGGIARAPQACGGPPGMLDSGSPGADAAIPGCP
                     DPVTVLAMPLDSAVEAADNRRAGDLQIFIEQPPADSGDQLSIAIAGPGMKGELESPST
                     RTDGYVLSTDIAPTILRHFGIKTPKAMTGQPIESGGKLDVGRLTDLENRYEQVGKRRG
                     AALAIPLLLWIALAGLVSFGSRGLYARPALRLLCLSTVLLPVVLLLTAALEPSLFVER
                     TIATLLPVLVAALLMRIAPGWLSLAIACGLTVIPYGIDMLAGSALTPRAVIGPNPGLG
                     ARFYGIGNELESTLMILTSIGTGASLTWFGTTGRRAAIWFLAVGTVATVVFAAGRFGA
                     DVGAAIVFPVAAVVAAAFAVGRPRLAWAGLAAALAALVLVALADVVTGSETHFVRSIV
                     GGTGGSFFEVIGHRLDATLESFTRVSRIPVTLVAVAVIALGIWQRSTVLSWIDELPMM
                     KAAAAAAAAGSLVGALTNDSGALFIQVGTLYLALIAGFAWTASKYHPESG"
BASE COUNT          517 a          915 c         1016 g          425 t
ORIGIN      
        1 ggccaggatc cggtggacgt catccgggtc ggccccgacc actgtgagcc gggcgggggc
       61 gtgttcgacc aaggcggaaa aagcgcgaag cagcaccggc aggcccttgc gttcgtcggg
      121 gcgaccgacg aagagcgcac gcagatggtc ggtctcgggc ttggggccgg tcggcgcggc
      181 atcgatgtcg actccgttgg ggacgatcgt gtaattgccg ccaaaccagc gtcgcccggt
      241 ccaggcggcc gcctgggata cggcgatgcg ggcggagagc cggttcaggg tgcgccgggc
      301 gccgaccgcg ttggcgatgt aattcggcaa cggcttggtc gaataggcgt ggaaggtgcc
      361 gaccaccgga acgtcgctcg acaggcaggt gtcccagccg ttggccggcg tggtcggctc
      421 gtggaggtgg acgacgtcga agtcgccttc ctcgatcgcg cgacgggtct ccaccacgcc
      481 cgaggggaac agcggaatgt tcgaaaccgc tccgttcgcg ttgatgccga aggtgcgccc
      541 gaccgggatc aggtagtccg ggatctcgcg gtcttccgcc tcggcacggt gaagcacctt
      601 cgagaggcgg tcgggtggat caaagggcgc gatgacacgg acgtaatggc ctcgggagat
      661 gtactcctcg gcgagcgact ggacgtgccg gtttacgccg ccctggtaag tccacgaata
      721 cggtgtgaca agggcgattc gcacgataga taaggatagt ggaggtggcg gtcctgggag
      781 gggcggttaa cccgattcag ggtggtattt cgacgccgtc cacgcaaatc ccgctatcag
      841 cgccagataa agcgtcccca cctgaatgaa aagtgccccg gaatcgtttg tcagggcgcc
      901 caccagggag cctgccgcgg cggccgcggc ggccgccttc atcatcggca attcgtcgat
      961 ccaggagaga acagtgctgc gttgccagat ccccagggcg atcacagcga ccgcgaccag
     1021 ggtgaccggt atccgggaaa cccgcgtgaa gctctcgagg gtggcgtcga gccggtgtcc
     1081 gatcacctcg aagaaggagc ccccggtgcc gccgacgatc gaacggacga aatgggtctc
     1141 gctgccggtg accacatcgg ccagggcgac caggaccagg gcggcgagag cggcggcgag
     1201 gccggcccag gccagtcgcg gccgaccgac cgcgaaggcc gcggctacga ctgcggcgac
     1261 gggaaagaca atggccgctc cgacgtcggc gccgaaccgg ccggcggcga atacgaccgt
     1321 ggccacggtt ccgaccgcga ggaaccagat cgcggcacgg cggcccgttg tcccgaacca
     1381 ggtcagggat gcgccggtgc cgatcgaagt gagaatcatc aaggttgact cgagttcgtt
     1441 gccgatcccg tagaagcggg ccccgagccc cgggttggga ccgatcaccg cacgcggcgt
     1501 gagcgccgac ccggccagca tgtcgatgcc gtaagggatc acggtgagac cgcaggcgat
     1561 tgcgagcgag agccatcccg gagcgatacg catcaacagg gcggcgacga ggacgggcag
     1621 cagggtggcg atcgttctct ccacgaaaag cgacggttcc agggccgcgg tcaggagcag
     1681 gaccaccgga agcagcacgg tcgacaggca gagcagacgc agggccggtc tcgcgtagag
     1741 gccgcggctg ccgaaggaga cgagcccggc cagcgcgatc cacagcagga gggggatcgc
     1801 cagggccgcg ccacggcgct tgccgacctg ttcgtagcgg ttttcgaggt cggtcagccg
     1861 accgacgtcg agcttgcccc cggattcgat cggctgcccg gtcattgcct tcggggtctt
     1921 gatcccgaag tggcggagga tcgtcggagc gatatcggtg gagagcacgt agccgtcggt
     1981 gcgggtgctt ggcgactcga gttcgccttt catcccgggt ccggcgatcg cgatcgaaag
     2041 ctggtcgccg gaatcggccg ggggctgttc gatgaagatc tgcaggtcac cggcccgccg
     2101 gttgtcggcc gcctccacgg cggagtccag gggcatcgcg agcacggtca ccgggtcagg
     2161 gcagccggga atcgcggcat ctgccccggg ggagcctgaa tcgagcattc ccggggggcc
     2221 gccgcaggcc tgcggagccc ttgcgatccc cccgtcccgg cgggccaccg ccagcgccgc
     2281 cgcacctgcc gagtccgctg ccaccgcatt gaggtcgtga ccggccagca cgctggccat
     2341 cagacccgga atcagggtgg tgtcgttgct cttcgccctc ctgaccacct tttcccatcc
     2401 ctgtgcgaat tccccgcccg gatacacccg gtctaacgga gtgtcatacg ttgagtcaaa
     2461 cgcccttgct ccctgcgaca cgtcgagcca ggtctgctgg gccgaaacgt cccccagacc
     2521 ggggttcatg agtccgatcg cggcatgttc caggccggcc agatcggccg gtgaggttcc
     2581 ctgaggcagg acgacgatcg tcacctgccc ttcgtggccg gttgccgcgt cactcaccgc
     2641 caggtcgtcg gccgacgccg gaacagcgag gaggaacagg gctacaaggc acaaaagcat
     2701 ggtcccgggc atcgaaatgg ccctggatcc ggcgaaaaac gccggcccat ggctcctccc
     2761 cggtatcgtc aggtccggca tgacaacgga aacgataggt cagaacgctc tcgcaatccc
     2821 cgaagtcgaa ttcgcccgag tcggcgaggt ggaaatcgca ttcgacgtcc acg
//
LOCUS       DAOTIP010000203         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41255_length_2870_cov_3.117584, whole genome shotgun sequence.
ACCESSION   DAOTIP010000203 DAOTIP010000000
VERSION     DAOTIP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41255_length_2870_cov_3.117584"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="P5138_04795"
     CDS             <1..677
                     /locus_tag="P5138_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRV59925.1"
                     /translation="LVAGALIAVAWAQLGIHAESVADFLGLGMTGTTAHRIDLATGIQ
                     FPFWAFSFALGMAGAKLYVEVRKRWEEGDCRRRAAWAQLAGLAGLVLFTILIYSSPEN
                     SRGIAVVAEGVRHSPLIMLGYSASLATLMVATALGPDWTRRPFDNRPMKWLAEVSYGA
                     YLSHALFLFIGTYSIGATFQGNVQTLLAWSAAILPATILYGYLSARYLEQPIRRWARK
                     FGRNVT"
     gene            complement(674..1552)
                     /locus_tag="P5138_04800"
     CDS             complement(674..1552)
                     /locus_tag="P5138_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV59926.1"
                     /translation="MTDGSPTAAAPASGGPDPGLQEVTGPSALGGGWRRSRDLLYLIA
                     VNDFKKTYFDTVLGYVWSLFRPLILFAVLLFVFTQIFRIGDDVPHYPVMLLFNIVVFT
                     FFQEATLAAVTSVVAQEGVVRKTQFPRLVIPLAVVLTALFNFGVNLVVVLVFLLASGV
                     PVEWTWLFFPFIVAILTVITVAVSLLVSALFVRFRDVGIIWGVLATALFYATPVLYPI
                     EVVPERFHEIVLINPLTPIFIELRHLVIDNSAPTALDAAGGWLGLLPAFLITVALCLI
                     AAKVFSREAPRIAEEL"
     gene            complement(1545..2840)
                     /locus_tag="P5138_04805"
     CDS             complement(1545..2840)
                     /locus_tag="P5138_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV59927.1"
                     /translation="MITDPLVETQTRDLSANPVVISVEGLKKSFNIPLHRVESFKERT
                     VHPLRKTEYRELKALDGIDFEVRKGEFFGIVGRNGSGKSTLLKILASIYRADAGSVKM
                     AGRLAPFIELGVGFNHDLTAHDNVIMNGVMMGLSRQEAAARLDRVVEFAELDDFKELK
                     LKNYSSGMLVRLAFSLMIQADADIMLIDEVLAVGDASFQQKCADVFNRMRDAGKTIVL
                     VTHDMAAVESFCDRAMLIEGGHIVEAGEPRAVARAYLKLNFDAETPRESTLGFHEEGE
                     IEDDFIPGVDIQARLIDSWLENEEGKRVTNVEIGDPLRLNVQIQALRDLNRPLFTFQI
                     NDAKHTQVTGFNQTLEVDGPGDVIREGERVTISGEVVNRLTPGRYLLTCWVNRNRESS
                     ERVIQGIKLFEFVVYGMKRTPGLVEAEADLDVKVSLEDD"
BASE COUNT          523 a          912 c          936 g          499 t
ORIGIN      
        1 gcctcgtggc cggcgccctg atcgccgtag cctgggcaca gctcggaatc cacgcggaaa
       61 gcgtcgccga cttcctcggg ctcggtatga ccgggacgac cgcgcaccgg atcgacctgg
      121 cgaccggcat ccagtttccg ttctgggcct tctccttcgc cctcggcatg gccggggcga
      181 agctctatgt cgaagtgaga aagcgctggg aggagggcga ttgccgccgt cgcgccgcct
      241 gggcccaatt ggcggggctg gccggtctgg tcctcttcac gatcctcatc tacagctcgc
      301 ccgagaacag ccggggcatc gccgtggtcg ccgaaggcgt gcggcattcg cccctgatca
      361 tgctcggcta ctccgccagt ctcgccaccc tgatggtggc gaccgcgctc ggacccgact
      421 ggacgcgccg gccgttcgac aaccggccga tgaaatggct ggccgaggtc agctacggcg
      481 cctatctctc ccatgcgctc ttcctgttca tcggcaccta cagcatcggc gccaccttcc
      541 agggcaacgt ccagaccctg ctcgcctgga gcgcagcaat cctccccgcc acgatcctct
      601 acggctacct gtcggccaga tacctggaac agccgatccg ccgctgggcc cggaagttcg
      661 gccggaacgt tacctagagc tcttccgcga tccggggagc ttcgcggctg aagaccttgg
      721 ccgcgatcag acagagggcg acggtgatca ggaaggcagg cagcaggccg agccagccgc
      781 cggcggcgtc gagggcggtc ggtgccgagt tgtcgatcac caggtgccgc agctcgatga
      841 agatcggggt gagcgggttg atcagcacga tttcgtggaa gcgctcggga acgacctcga
      901 tcgggtagag gaccggggtc gcgtagaaca gcgcggtggc gagaacgccc cagatgatgc
      961 cgacgtcccg gaaccgcacg aagagggcgg agaccaggag gctgacggcg acggtgatca
     1021 cggtcaggat cgccacgatg aaggggaaaa acagccaggt ccattcgacc ggcacccccg
     1081 aggcgagcag gaagaccagc acgacgacga ggttcacgcc gaagttgaac agggcggtga
     1141 ggaccacggc caacgggatc accagccggg ggaactgggt ctttcggacc accccctcct
     1201 gggccaccac cgaagtcacg gcggcgaggg tcgcctcctg gaagaaggtg aagacgacaa
     1261 tgttgaacag cagcatcacc gggtaatgcg gcacgtcgtc tccgatccgg aagatctggg
     1321 tgaagacgaa gagcagcact gcgaacagga tcagcggccg gaacagcgac cagacatagc
     1381 cgaggaccgt gtcgaagtag gtcttcttga agtcgttgac cgcgatcagg tagagcaggt
     1441 ccctcgaccg gcgccatccg ccgccgaggg cggaggggcc ggttacctcc tgcaggcccg
     1501 ggtcgggccc gcccgaagcc ggggcggctg cggtcgggga gccgtcagtc atcttccagg
     1561 ctcaccttca cgtcgaggtc ggcctcggct tcaaccaggc cgggggtgcg tttcatcccg
     1621 tagacaacga actcgaagag ctttattccc tggatcaccc gctcggagga ttcccggttg
     1681 cggttcaccc agcaggtcaa caggtagcgg cccggggtga ggcggttcac cacctcgccg
     1741 ctgatcgtga cccgctctcc ctcacggatc acgtcaccgg gaccgtccac ttccagggtc
     1801 tggttgaagc cggtcacctg ggtgtgcttc gcgtcattga tctggaaggt gaagagggga
     1861 cggttcagat cccgcagagc ctgaatctgc acgttgagcc gcaacgggtc accgatctcc
     1921 acgttcgtga cccgtttgcc ttcctcgttt tcgagccagg agtcgatcag tcgggcctgg
     1981 atgtcgaccc cgggaatgaa gtcgtcctcg atctcaccct cttcgtggaa gcccagcgtc
     2041 gactcgcgcg gggtttcggc gtcgaagttc agtttgaggt atgcgcgggc gacggcgcgg
     2101 ggctcaccgg cctcgactat gtggccgccc tcgatcagca tcgcccggtc gcagaaggat
     2161 tcgaccgcgg ccatgtcatg ggtgaccagg acgatcgtct tgccggcatc gcgcatccgg
     2221 ttgaagacgt cggcgcattt ctgctggaag gaagcgtcgc cgaccgccag tacctcgtcg
     2281 atcagcatga tgtcggcgtc ggcctggatc atcagcgaga acgcgagccg gaccagcatt
     2341 cccgaggaat agttcttgag cttgagttcc ttgaagtcgt cgagctcggc gaactcgacc
     2401 acccggtcga gacgggccgc tgcctcctga cggctcagac ccatcatcac gccgttcatg
     2461 atcacgttgt catgggccgt gagatcgtgg ttgaaaccga ccccgagctc gatgaacggg
     2521 gcgagacgac cggccatctt caccgatccg gcatcggccc ggtagatcga ggcgaggatc
     2581 ttcaggaggg ttgacttgcc ggaaccgttg cgcccgacga tgccgaagaa ctcgcccttt
     2641 ctcacctcga agtcgatccc gtcgagcgcc ttcagttcgc ggtactcggt ctttcgcagc
     2701 gggtggaccg tcctctcctt gaacgactcg acccggtgca gagggatgtt gaagctcttc
     2761 ttgagcccct ctaccgagat caccaccgga ttggcggaga ggtcccgtgt ttgtgtttcg
     2821 acgagcggat ccgtgatcat ggaattcggg ggctaaccga gaaagccgct
//
LOCUS       DAOTIP010000204         2865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41347_length_2865_cov_3.195730, whole genome shotgun sequence.
ACCESSION   DAOTIP010000204 DAOTIP010000000
VERSION     DAOTIP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41347_length_2865_cov_3.195730"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="P5138_04810"
     CDS             complement(<1..1353)
                     /locus_tag="P5138_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV59928.1"
                     /translation="MKSLLLLPAGPRARWITVAVWFIAMFVVFGTNIPGKFADAENNE
                     SISYLPGDAEATRALEAQKKLVGSEEAPIVIVYRREGGLTAADRERIRENLAELNSQV
                     EGVSKPFVLTQKSKDGTAVIVTSSITSTGEGEDIREPVEDARDIVSGNFDGLEVAITG
                     PAGYASDSIKVFESINGTLFAAAFGLVFVLLILIYRSPFLLWLPLISVGFAEIGARAV
                     GYGLTEMGVTVNGQSSSILSVLVLGAGTDYALLLISRYREELRHRESRTESMQVALGS
                     AGPAIVYSGLTVIAALLCLSLAELNSTASMGPIGAMGILMAMISMLTLLPAMLVIAPR
                     FVFWPKVPRVGTGGADAEHGPWRRLGDRIAKRPRPVWITAVLVLLVMCAGLFSYNDGL
                     TSANGYVDEVEAVRGEQTIAKSFPGGSNVPTEIVVGDPSRVSAVAKAVGDVPGVAGVR
                     K"
     gene            1378..1914
                     /locus_tag="P5138_04815"
     CDS             1378..1914
                     /locus_tag="P5138_04815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRV59929.1"
                     /translation="MERKKLGVLIGLFVLVAALVAVILIGRGSGDDSGGSASGEVPSA
                     VIADVSEDLDTRPKLAGSTETPPTTLVKKDIVVGDGTEAKAGDTVKVQYVGALFNNGN
                     EFDASWDRGNQPFSFTLGSGQVIKGWDEGVEGMKVGGRRVLVIPPDLGYGAQGSPPTI
                     PANSTLVFVVDLEDVQSQ"
     gene            1923..2423
                     /locus_tag="P5138_04820"
     CDS             1923..2423
                     /locus_tag="P5138_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRV59930.1"
                     /translation="MGVIDDLVENNRAFSARVPDQHLDVKPSSKLTIVTCMDSRLDVF
                     QALGLEDGQAHILRNAGGVITDDVIRSLSISQRKLGTESIMLIHHTDCGMQKITDDGF
                     RSELQEATGTAPAFAIESFTDVERDVAQSIRRVRRSDFIPHRSNVRGFVYNVDDHALT
                     EVVVED"
     gene            2450..2785
                     /locus_tag="P5138_04825"
     CDS             2450..2785
                     /locus_tag="P5138_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRV59931.1"
                     /translation="MAITVYEKPTCTTCRKLYRLLEAEGVDFDKVNYHVTGLTEDEIR
                     DLLGKADITAAEALRMREDGARDLASADEDEIIAAMAERPELLQRPIVVNGDRAVLAR
                     PIERALEVL"
BASE COUNT          527 a          964 c          904 g          470 t
ORIGIN      
        1 tttacggacc cccgccaccc ccggcacgtc tccgaccgcc ttcgcgactg ccgaaacccg
       61 ggaggggtca ccgaccacga tctcggtcgg cacgttcgag ccgccgggga aggacttggc
      121 gatcgtctgc tctccccgga ccgcctccac ttcgtccacg tacccgttcg ccgaggtcag
      181 gccgtcgttg tagctgaaga gcccggcgca catgaccaga aggaccagca ccgcggttat
      241 ccagaccggc cggggacgtt tggcgatccg gtcgccgagg cgacgccagg ggccgtgctc
      301 cgcgtcggcc ccgccggtcc cgacccgcgg caccttgggc cagaagacga accggggagc
      361 gatcaccagc atcgccggca gcagggtcag catcgagatc atcgccatca ggattcccat
      421 tgcgccgatc gggcccatcg aggcggtcga gttcagctcg gcgagcgaga ggcagagcag
      481 cgcggcgatc acggtcaggc cggaatagac gatcgccggc ccggcgctgc ccagggcgac
      541 ctgcatcgat tcggttctgc tttcgcgatg tctgagttcc tcccgatatc gactgatcag
      601 cagcagcgcg tagtcggtcc cggcccctaa cacgaggacc gagaggatcg acgaagactg
      661 accgttcacg gtcacaccca tttcggtcag gccgtagccg accgcccggg ccccgatctc
      721 ggcgaagccg accgagatca gcggcagcca cagcaggaac ggcgagcggt agatcaagat
      781 cagcagaacg aagaccagcc cgaaggccgc ggcgaaaagg gtcccgttga tcgactcgaa
      841 gaccttgatc gagtcggagg cgtagccggc aggtccggtg atcgccactt ccaacccgtc
      901 gaagttgccg ctgacgatgt cgcgggcatc ctcgaccggc tcgcggatgt cctctccctc
      961 cccggtcgag gtgatcgagg aggtgacgat aaccgccgtt ccgtccttcg atttctgggt
     1021 cagcacgaag ggcttgctga ccccttcgac ctggctgttc agttcagcca ggttctccct
     1081 gatccgctcc cggtcggcgg cggtcaggcc gccctcacgc cggtagacga tcacgatcgg
     1141 ggcctcttcg gatccgacca gcttcttctg cgcttccagc gcccgggtgg cttcggcgtc
     1201 acccggcagg tacgagatcg actcgttgtt ttcggcgtcg gcgaacttgc cggggatgtt
     1261 cgtcccgaaa acgacgaaca ttgcgatgaa ccagacagcg actgttatcc agcgggcccg
     1321 gggcccggcg ggcaggagga ggagagactt catgggcgag acactagtat gcccgccatg
     1381 gaacgcaaga agctcggggt tctcattggc ctgttcgtat tggttgccgc tctggttgcg
     1441 gtcatcctga tcggccgcgg cagcggcgac gattcgggtg gctcggcatc gggcgaggtc
     1501 ccgagcgcgg ttatcgccga cgtctcggag gatctcgaca cgcggccgaa actcgccggc
     1561 tccacggaaa ccccgcccac gaccctggtc aagaaggaca tcgtggtcgg tgacggcacc
     1621 gaggccaagg caggagacac ggtcaaggtc cagtacgtcg gtgcgctctt caacaacggc
     1681 aatgaattcg acgcctcctg ggaccgcggc aatcagccct tcagcttcac gctcggcagc
     1741 ggccaggtca tcaagggctg ggatgagggc gtcgaaggca tgaaggtcgg tggccgccgg
     1801 gtgctggtca ttccgcccga cctcggttac ggagcgcagg gcagcccgcc cacgattccg
     1861 gccaactcca ccctggtctt cgtggtcgac ctcgaagacg tccagagcca gtagaagcgt
     1921 cgatgggagt aatcgacgac ctggtcgaga acaaccgggc cttttccgcc cgggttcccg
     1981 accagcatct cgatgtcaag cccagcagca agctgaccat cgtcacctgc atggattccc
     2041 ggctcgacgt cttccaggcc ctcggtcttg aggatggcca ggcccacatc ctgcgcaacg
     2101 ccggcggggt gatcaccgat gacgtgatcc gctcgctctc gatctcccag cgcaagctcg
     2161 gcaccgagtc gatcatgctt atccaccaca ccgactgcgg catgcagaag atcaccgacg
     2221 acggtttccg ctccgagctc caggaggcaa ccggcaccgc gccggccttt gcgatcgagt
     2281 cgttcaccga cgtcgagagg gacgtcgccc agtcgatccg ccgggtccgg cgttccgact
     2341 tcatcccgca ccggtcgaat gtgcgcggct tcgtttacaa cgttgacgac cacgccctga
     2401 ccgaggtcgt ggtcgaggac tgaagagagg tccggaccgg gaagtcgcga tggcgatcac
     2461 cgtctacgaa aaaccgacct gcaccacctg ccgcaagctc tacaggctgc tcgaggccga
     2521 aggggtcgat ttcgacaagg tcaactacca cgtcaccggc ctgaccgaag acgagatccg
     2581 cgacctgctc ggcaaggcgg acatcacggc tgccgaggcc cttcgcatgc gtgaggacgg
     2641 cgcccgggat ctggcgtccg cggacgagga cgagatcatc gccgccatgg ccgaacggcc
     2701 agaactgctg caacgcccga tcgtcgtcaa cggcgatagg gccgttctcg caaggccgat
     2761 cgaacgcgct ctggaagtcc tctagtccgc tcccttcttg ccgacgtgcc cggccgggta
     2821 ccctcggttc gtgagcattc cgagcatcga cctcaacgac ggaca
//
LOCUS       DAOTIP010000205         2854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41541_length_2854_cov_3.779921, whole genome shotgun sequence.
ACCESSION   DAOTIP010000205 DAOTIP010000000
VERSION     DAOTIP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41541_length_2854_cov_3.779921"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..676
                     /locus_tag="P5138_04830"
     CDS             110..676
                     /locus_tag="P5138_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRV59932.1"
                     /translation="MSGALIALIIVVVIALISVLWYISTRNGIIGSRNRVEESWSGID
                     VQLKRRHDLVPNLVETVKGYATHEQKVFEEVTKARADAMAAQSVGDTAQAEAGLNRSL
                     ADLRAVAEQYPDLKATQNFQQLSRNLSELEDEIQASRRIYNSNVQSYNTKIQQFPASI
                     VANQGGFTAREFFEIEDAAERATPNVSF"
     gene            complement(673..1353)
                     /locus_tag="P5138_04835"
     CDS             complement(673..1353)
                     /locus_tag="P5138_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV59933.1"
                     /translation="MPTSLERRHNRRRRHERTRQELIEAALNQSAAGSFKDLTVEGVT
                     REAGVARSAFYVYFGDKEELLLGALEDLIASHQNRLGRCWQQGDDPRRDIERGIYGMA
                     RIYSESSALLALAGEAATYDEEVRELWSTVLETITEGTREEIERLQRSGAVSDSLDPE
                     ALAEGLVLMTERSFQVHLAQGEGDPNEVASSLTRVWWAALFGPPEQEKTPSPPAGEEG
                     AETAVAEG"
     gene            complement(1394..2224)
                     /locus_tag="P5138_04840"
     CDS             complement(1394..2224)
                     /locus_tag="P5138_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRV59934.1"
                     /translation="MRFPGRKFRAALLVLAVASAPALIAAPARATSTWVSISDFQWSN
                     KNPHIDLGESVTWTWAGPDTAHSVTGKAPTDSAFDPILTDDASQWDSDAGNNSPNHWP
                     GDEFKVTFDHPGTYLFACKLHPVVRGEVTVSNVPGDPNSDPGPKPNINFDFESPVVTD
                     YFFTSDGTIPIPAVAGPKGKGIGFRFSINEKATVSADYYQLIRRGKGKKRRTIRVFRG
                     YSQWDAHIGFNKVGFAKRSSDFAARPGRYLAYFRAEDRVQNATKDIPVRFTIKGRKRR
                     "
     gene            complement(2227..>2854)
                     /locus_tag="P5138_04845"
     CDS             complement(2227..>2854)
                     /locus_tag="P5138_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRV59935.1"
                     /translation="GQPPTTPDFGTGLTNDASQWDSDPNTNYPSHVPGSEYDVTFDHP
                     GTYLFVCKVHQSVRGTVTVSDTAGNPDSDFGPAPAINFDIDPPHIDRYYFTTDGTSIA
                     APNIGPKGKGIGFAYSTNESGTASVDYYRIVKKGKGKKRKKLKFFQGYNEWPAHIGFN
                     VARFAARSSTFKPKAGKYVAYFRVEDLESNSTKDITLRFTIFGKKKKR"
BASE COUNT          482 a          872 c          954 g          546 t
ORIGIN      
        1 tgccactggt ccgattcctt gcccatcggc gaccacccta cccgtagtaa gaaggtgact
       61 ctgcggatag gatggccgcg atgctcattt caaccgagga gatttgccta tgagtggcgc
      121 actgatcgcg ctgattattg tcgtcgtaat tgccctgatt tcggtgctct ggtacatctc
      181 gacgcgcaac gggatcatcg gttcgcgcaa ccgggtcgag gagtcctgga gtggcatcga
      241 cgtccagctg aagcgccgac atgacctggt cccgaacctg gtcgagacgg tcaagggata
      301 cgcgactcac gagcagaagg tcttcgaaga ggtgaccaag gcccgtgccg acgcgatggc
      361 cgcccagagc gtcggggaca ccgcgcaggc ggaagccggc ctgaaccgtt cgctggccga
      421 cctgcgggcg gtcgccgagc agtacccgga cctgaaggca acccagaact tccagcagct
      481 gagccggaat ctctccgagc tcgaggacga gatccaggct tcccgccgga tctacaactc
      541 gaacgtccag tcctacaaca caaagatcca gcagttcccc gcttcgatcg tcgccaacca
      601 gggcggcttt accgcgaggg agttcttcga gatcgaggac gccgccgagc gcgcgacccc
      661 gaacgtcagc ttctagcctt cggcaacagc tgtttcagcg ccctcttcgc cggccggcgg
      721 ggagggcgtt ttttcttgtt ccgggggacc gaagagggct gcccaccaga cccgggtgag
      781 gcttgatgcg acctcgttgg ggtcgccttc gccctgggcg aggtggacct ggaagcttcg
      841 ctcggtcatc aggaccagac cttcggccag cgcctccggg tcgagcgagt cactgaccgc
      901 gccggaccgc tggagccgct cgatctcctc gcgggtgcct tcggtgatcg tctccagcac
      961 ggtggaccag agctcgcgca cctcttcgtc gtaggtggcg gcctcgccgg ccagggcgag
     1021 cagggccgaa ctctccgaat agatgcgggc catcccgtag atcccgcgct cgatgtcgcg
     1081 tcggggatcg tcaccctgct gccagcagcg gccgagccgg ttctggtgcg aggcgatcag
     1141 gtcctcgagc gccccgagca acagctcctc cttgtccccg aaatagacgt agaaggccga
     1201 gcgggcgacg cccgcctcgc gggtcacgcc ctcgaccgtc aggtccttga acgagccggc
     1261 ggcggactgg ttcagcgcag cctcgatcag ctcctgtcgg gtccgctcgt gacggcggcg
     1321 gcggttatgt cggcgctcaa gcgaggtcgg cacccgccaa gcataggtgc cgaccccgac
     1381 gggtcggtca ccgttatcgg cgcttcctgc ccttgatcgt gaagcggacc ggaatgtcct
     1441 tcgttgcgtt ctggacccgg tcctcggcgc ggaaataggc gaggtagcgg ccgggacggg
     1501 cggcgaagtc actggaacgc ttcgcgaatc cgaccttgtt gaacccgatg tgtgcatccc
     1561 actggctgta gccccggaag accctgatcg tgcgcctctt cttgcccttg ccgcgcctga
     1621 tcagctggta gtagtcggcc gagacggtcg ctttctcgtt tatcgagaat ctgaagccga
     1681 tgccctttcc cttcgggccg gcaacggccg ggatcgggat ggtgccgtcc gaggtgaaga
     1741 agtaatcagt gaccaccggt gattcgaagt cgaagttgat attcggcttc gggcccggat
     1801 ccgagttggg gtcacccggg acgttcgaga ccgtcacctc tcccctcacc accgggtgga
     1861 gcttgcaggc gaagagatag gtgccagggt ggtcgaaggt gaccttgaac tcgtctcccg
     1921 gccagtgatt tggcgagttg ttgccggcat ctgaatccca ctggctggca tcgtcggtca
     1981 ggatcgggtc aaaagccgag tcggtcggcg ccttgccggt gaccgaatgg gcggtatccg
     2041 gaccggccca ggtccaggtc acggactctc cgaggtcgat gtgggggttc ttgttcgacc
     2101 actggaagtc ggagatcgac acccaggtgc tagtcgcgcg ggccggcgct gcgatcaggg
     2161 cgggcgccga ggcaaccgca agtaccagga gggcagcgcg gaacttcctg ccggggaacc
     2221 tcacgctcag cgctttttct tcttgccgaa gatcgtgaag cgcagggtga tgtccttggt
     2281 cgagttcgac tcgagatcct cgacccggaa gtaggcgacg tacttgccgg ccttcggctt
     2341 gaaggtgctc gaccgggcgg cgaaccgggc aacgttgaag ccgatgtggg cgggccactc
     2401 gttgtagccc tggaagaact tgagcttctt ccttttcttg cccttgccct tcttgacgat
     2461 ccggtaatag tcgaccgagg cggttccgct ctcgttggtg gagtaggcga aaccgatccc
     2521 cttgcccttc ggaccgatgt tcggggcggc gatggaagtg ccgtcggtgg tgaagtagta
     2581 ccggtcgatg tgcggaggat cgatgtcaaa gttgatcgcc ggagcaggcc cgaagtcgga
     2641 atccggatta cccgcggtat ccgagaccgt gaccgtgccg cgcaccgact ggtggacctt
     2701 gcatacgaac aggtaggtgc ccggatggtc gaaggtcacg tcgtattcgc tgccggggac
     2761 gtggctcgga tagttggtgt tcgggtccga gtcccactgg ctggcatcgt tggtgaggcc
     2821 ggtgccgaaa tcgggggtgg tcggaggctg gccg
//
LOCUS       DAOTIP010000206         2850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41614_length_2850_cov_3.002147, whole genome shotgun sequence.
ACCESSION   DAOTIP010000206 DAOTIP010000000
VERSION     DAOTIP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41614_length_2850_cov_3.002147"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="P5138_04850"
     CDS             complement(<1..773)
                     /locus_tag="P5138_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRV59936.1"
                     /translation="MATAIRFSVDGEILEPGEAFLPLPDDGLLRGDGVFEVARIYGGR
                     PFALDLHFDRMERSAAAIGLDLERDLLEGDADRLITSNGDADCLIRIVQTRSGRRILS
                     LEELPDHSPTISLASVSYSPTVVLNGVKSLSYAANMHATRLAKEAGAEEALFVSPEGI
                     VLEPPTSSIFWATADGLLRTPTLEAGVLDSITRRKIVERLEVEEGEFPLEDLLEASEA
                     FLASTTREAHPVSRIDGHEMPVVDGEWTGAARNAFAEALT"
     gene            complement(811..1380)
                     /locus_tag="P5138_04855"
     CDS             complement(811..1380)
                     /locus_tag="P5138_04855"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRV59937.1"
                     /translation="MDFKAIDLPGLLLDTESAVAMRAHGKTEAAVLMPISNWPHAPRL
                     TFTERRADLRKHAGEISFPGGTTEPQDRDLEETALRESEEEIALHRVHVDVIGAVPPV
                     GTFATSFRIQPIVGLVSEEATMVANPEEVATILTFGFDELLDSYAMRRLVRRGVPIRT
                     PTFEIGRHLIWGATARILTDFLHRIGAMN"
     gene            1495..2019
                     /locus_tag="P5138_04860"
     CDS             1495..2019
                     /locus_tag="P5138_04860"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRV59938.1"
                     /translation="MDHASLIRSIPDFPEPGILFRDITPLLLDAGACRAVTRDLAEWA
                     RPLEAEYLVAAESRGFIFGGALAQELDIGFIPARKPGKLPAETVSVEYELEYGLDALE
                     VHADALSDGARVLVHDDLIATGGTAQAKIEAVEKLGAEVVGCAFLIELEGLGGRDRIA
                     PHETHALLKLPAGG"
     gene            complement(2067..>2850)
                     /locus_tag="P5138_04865"
     CDS             complement(2067..>2850)
                     /locus_tag="P5138_04865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV59939.1"
                     /translation="GRVCVVTGASGGIGLEVTRQLCDEGAKVLMVSRDPEQLAAMAEV
                     VRAEGGEVEVVVKDVTSDCAGQHIIAAGKQAFGRVDALVNNAGAAEWKPLEDASEEDF
                     RFQFELSVIAPLNLMRAAIPEMTDRGWGRVVNVSSTAGKRPSSMMPDYSVGKAAMLSL
                     SRQFAERHAAEGVMVNAICPGPTASDLWMAEGGLLDQSKEAAGLESREEALKAAGEKR
                     PIGRLATPEEIASAIVFLCSERASYVSGAAWGIDGGTVPVII"
BASE COUNT          465 a          956 c          967 g          462 t
ORIGIN      
        1 gtcagggcct cggcgaaggc gttccgggct gccccggtcc actcgccgtc aaccaccggc
       61 atctcatggc cgtctattcg cgagaccgga tgggcttcac gggtggtcga ggccaggaag
      121 gcctcggagg cctcaagcag gtcttcgagc ggaaactccc cctcctcgac ctcgagccgc
      181 tcgacgatct tgcgccgggt gatcgagtcg agcacacccg cttccagggt gggggtacgc
      241 agaaggccgt cggcggtcgc ccagaagatc gacgaggtcg gaggctcgag aacgatgccc
      301 tccggactga cgaagagcgc ttcctcggcg ccggcctctt tggccagcct ggtggcgtgc
      361 atgttggcgg cgtaggaaag cgacttgacc ccgttgagca ccacggtcgg cgagtagctg
      421 accgaggcca gggagatcgt gggactgtga tccggcaact cctccagcga gaggatgcgt
      481 cggcccgacc tggtctgaac gatccggatc aggcagtccg catccccgtt ggaggtgatc
      541 agccggtcgg cgtccccttc gagcagatcc cgctcgagat ccaagccgat cgcggcggcc
      601 gaacgttcca tccggtcaaa gtggagatcg agggcgaagg gacggccccc gtagatcctt
      661 gccacctcga aaaccccgtc gccacgcagc agtccgtcgt ccggcagggg aagaaaggcc
      721 tctcccggtt cgagaatttc cccgtcgacg ctgaacctga ttgccgtcgc catctgaacc
      781 aagcatagaa ccgcgcccgg tcctcccgcc tcagttcatg gctccgatgc ggtgcaggaa
      841 gtcggtcagg atgcgggccg tggcccccca gatcaggtga cggccgatct cgaaggtggg
      901 ggtgcggatc gggactcccc gccggaccag tcgtcgcatc gcgtaggagt cgagcagctc
      961 gtcaaacccg aaggtgagga tggtcgcgac ctcttccgga ttggcgacca tggtcgcctc
     1021 ttccgagacg agcccgacga tgggctggat ccggaacgag gtggcaaagg tgccgaccgg
     1081 tggcaccgcg ccgatcacgt cgacgtggac gcggtgcagg gcgatctcct cctccgactc
     1141 gcgcagggcg gtctcttcca ggtcacggtc ctgcggctcg gtggtcccgc ccgggaagga
     1201 gatctcgccg gcatgcttgc gcaggtcggc cctgcgttcg gtgaaggtca ggcggggagc
     1261 atggggccag ttcgagatcg gcatcagcac cgcggcctcg gtcttgccat gggctctcat
     1321 cgccaccgcc gactcggtgt cgagcagcag cccgggcagg tcgatcgcct tgaagtccac
     1381 gccctgatca tgccgaaccg gtgggtccgg cgacggtagg ctggagcgga gcttcgctcc
     1441 gtcccggagc ggagcctccc gtgacatggc gaaagagagg agggtgggca gaaagtggat
     1501 cacgcatccc tgatccgttc gatcccggac ttccccgagc cggggatcct tttccgcgac
     1561 ataacgccgt tgctgctgga cgccggggcc tgccgcgcgg tcactcggga cctggctgaa
     1621 tgggcccgcc ccctggaggc cgagtacctg gttgccgccg aatcccgcgg tttcatcttc
     1681 ggcggagcac tggcccagga gctggacatc ggcttcatcc ccgcccgcaa gccgggcaag
     1741 ctgccggccg aaacggtctc ggtcgaatac gaactcgaat acggcctcga cgcgctcgag
     1801 gttcacgctg atgccctttc ggatggtgcc cgcgtgctgg tccacgatga cctgatcgcc
     1861 accggcggca ccgcccaggc gaagatcgaa gcggtcgaga agctgggggc ggaggtggtc
     1921 ggctgcgcct tcctgatcga actcgaaggc ctcggcggcc gcgatcgcat cgccccccat
     1981 gaaacccacg cgctgctgaa gctcccggcc ggcggctaga tgatcacggg gacggtgccg
     2041 ccggaagatc agggggacga cggcggctag atgatcacgg gaacggtgcc gccgtctatg
     2101 ccccaggcgg cgccagacac gtaggaggcc cgctcggagc agaggaagac gatcgccgag
     2161 gcgatttcct ccggggtggc cagccggccg atcggccgct tctcacctgc cgccttcagg
     2221 gcttcctcgc ggctttcgag tccggccgct tccttcgact gatcgagcag gccaccctcg
     2281 gccatccaga ggtcggaagc cgtgggcccg gggcagatcg cgttcaccat cacgccttcg
     2341 gcggcgtggc gttcggcgaa ctgacgggag agcgagagca tcgccgcctt gcccaccgag
     2401 tagtcgggca tcatcgaaga tggtcgcttg ccggcggtcg aggagacgtt gaccacgcga
     2461 ccccagccgc ggtcggtcat ctcggggatc gccgcccgca tcaggttcag gggagcgatc
     2521 acgctgagct cgaactggaa gcggaagtcc tcttcgctgg catcctcgag cggtttccac
     2581 tcggcggcgc ccgcgttgtt gaccagcgcg tcgacccgcc cgaaggcctg tttcccggcc
     2641 gcaatgatgt gttggcccgc gcagtcggag gtgacgtcct tgaccacgac ctcgacttca
     2701 ccgccttcgg cccggaccac ctcggccatg gctgccagct gctccgggtc gcgagagacc
     2761 atgaggacct tcgctccttc gtcgcagagc tgccgggtga cttcgaggcc aatgccgccg
     2821 ctggccccgg tgaccacgca gacccggccc
//
LOCUS       DAOTIP010000207         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41766_length_2842_cov_2.229279, whole genome shotgun sequence.
ACCESSION   DAOTIP010000207 DAOTIP010000000
VERSION     DAOTIP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41766_length_2842_cov_2.229279"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="P5138_04870"
     CDS             <1..359
                     /locus_tag="P5138_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59940.1"
                     /translation="QVKEAGKKIVTEDLRVIHHHSLELISEMEGWVETHMKLSRKWQH
                     LLPDNGGGEPDWEDRARRAEAELSATRLMAGAGEIIWEARTQDLERELEGVKNSTSWK
                     ITRPLRRLRGSREKNR"
     gene            complement(361..1113)
                     /locus_tag="P5138_04875"
     CDS             complement(361..1113)
                     /locus_tag="P5138_04875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV59941.1"
                     /translation="MRQEGKPPFPPLDLANRVSSLPDGPDAMNHYEWLGLMASQSINA
                     ALPDDWTWEGKRLLDFGCGAGRTLRHFLDVSGKAEVWGCDIDAPSIEWMQENLCPPLN
                     AVTCREEPPLEFESGSFDLIWAVSVFTHLAETSAEWMLEMHRLLKPEGILIASFMGRL
                     THEPLTGQPWDESGTGIKVIGLDNPWDLGGPSVFMSDWWVREHWGRAFEVEIIDAGVE
                     TLNQSWAVMRRKPVDLTAGELFEEKPRRWWRR"
     gene            complement(1136..2095)
                     /locus_tag="P5138_04880"
     CDS             complement(1136..2095)
                     /locus_tag="P5138_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59942.1"
                     /translation="MAPGKRVTDPLQWGYSLSNVFELLSGCLEAIEAKTVLEIGSYKG
                     ELTVELLEWANERGVKVAGVDPLPPDELEALAKEHPELDLLRQTGADVLVERDFDDAI
                     IIDGDHNYYTLSEELKIIGEQAPGAEMPLLMFHDVCWPHARRDTYYAPERIPEEYRQP
                     LVHDEGIAPGIKGTAEWGLPFVWAAAEEGGPKNGTMTAIEDFLTDKPGIRFAVVPAFF
                     GFGVMWHQDASWATAVERVVTPYDRHPVLERMEGNRVEHLVAGQARARQLIKLEEKVA
                     KQEDLLRRLLGSSAFTAAEKLSGLKQRGKPLFTREEVQSLLDE"
     gene            2126..2725
                     /locus_tag="P5138_04885"
     CDS             2126..2725
                     /locus_tag="P5138_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59943.1"
                     /translation="MDDLGEHNDAASLMADLRSRHPAFTAAVLADTVVTAQHRGERYE
                     FRSKADALLAALRLAWVSDAFLAQVFYRAKAACQRREVPVLPRLFHRLSMMLAQVSIG
                     DPVVMHPGVYIVHGQVVLDGIVEIHPGVVISPWVTIGLKAGNVVGPTVREGVNIGTGA
                     KLIGPIDVGTGAMIGANAVVVSDVPEGTTAVGVPAAKSE"
BASE COUNT          489 a          924 c          933 g          496 t
ORIGIN      
        1 tgcaagtgaa ggaagccggc aagaagatcg tgaccgaaga cctccgggtg atccaccatc
       61 actcgctcga gctgatcagc gagatggagg gctgggttga aacccacatg aagctctccc
      121 gcaagtggca gcacctgctt ccggacaacg gtggcggtga gcccgactgg gaggatcgcg
      181 cccggcgggc cgaagcggag ctttccgcca cccggttgat ggctggcgcg ggcgagatca
      241 tctgggaagc ccggacccag gacctcgagc gggagctgga gggcgtgaag aacagcacct
      301 cctggaagat cacccgcccc ctgcggcgcc tgcgcggcag ccgcgaaaag aaccgttagc
      361 tcagcggcgc caccagcgcc ggggcttctc ctcgaacaat tctccggcgg tcaggtcgac
      421 cggtttcctc cgcatcacgg cccagctctg gttcagggtc tcgaccccgg catcgatgat
      481 ctcgacctcg aaggcgcgcc cccagtgttc gcggacccac cagtcggaca tgaagacgga
      541 ggggccgccc aggtcccagg ggttgtcgag accgatcacc ttgatcccgg tgccgctttc
      601 atcccagggt tggccggtca gcggctcgtg ggtcagccgg cccatgaagc tggcgatcag
      661 gatgccttcg ggcttcagaa gccggtgcat ttcaagcatc cattcggccg aggtctcggc
      721 caggtgggtg aagaccgaaa ccgcccagat cagatcaaag ctgccgctct cgaattccag
      781 gggcggctct tcccggcagg tgacggcatt caggggagga cagaggttct cctgcatcca
      841 ttcgatcgaa ggggcgtcga tgtcacagcc ccagacctcg gccttccccg agacgtcgag
      901 gaaatggcgc agggtcctgc cggcgccaca tccgaagtcc agcaggcgct ttccttccca
      961 ggtccagtca tcagggaggg cggcgttgat cgactggctg gccatcagtc ccagccactc
     1021 gtagtggttc atggcatccg ggccgtcggg cagcgaactg acccggttgg ccaggtccag
     1081 tggcggaaac ggtggtttgc cttcctgcct caagtggcgt cccggacctg gcggtctact
     1141 cgtcgagaag ggactggact tcctcgcggg tgaagagcgg cttgcctcgc tgcttcagcc
     1201 ccgagagctt ctcggccgcg gtgaaggcgc tggatccgag cagcctccgc agcaggtcct
     1261 cctgcttcgc gaccttctcc tccagcttga tcagctgccg ggcgcgcgcc tggccggcga
     1321 ccaggtgctc gacccggttg ccttccatgc gctcgaggac aggatgccgg tcgtaggggg
     1381 tgacgacccg ttccaccgcg gtggcccatg aagcgtcctg atgccacatc accccgaagc
     1441 cgaagaaggc ggggaccacc gcgaaccgga tcccgggctt gtcggtcagg aagtcctcga
     1501 tcgcggtcat agtgccgttc ttcgggccac cctcttcggc agcggcccag acgaagggaa
     1561 gcccccattc ggccgtaccc ttgattccgg gggcgatccc ctcgtcgtgg accaggggct
     1621 ggcgatattc ctcggggatc cgctccgggg cgtaataggt gtcgcggcgg gcgtggggcc
     1681 agcagacgtc gtggaacatc agcagcggca tctccgcgcc cggcgcctgc tcgccgatga
     1741 tcttcagctc ttcgctcagc gtgtagtagt tgtggtcgcc gtcgatgatg atcgcgtcat
     1801 caaagtccct ctcgacgagt acatcggcgc cggtctggcg cagcagatcg agctccggat
     1861 gctccttcgc cagtgcctcc agctcgtccg gcggcagcgg gtcgaccccg gccaccttca
     1921 cgccgcgctc gttcgcccat tcgagcagct cgaccgtcag ctcacccttg taggagccga
     1981 tttcgagcac agtcttcgct tcgattgcct ccaggcaacc cgagagcagc tcgaagacat
     2041 tgctcagcga gtagccccac tggagtggat cggtaacgcg ttttccggga gccatcggcg
     2101 gcaagggtat agattcgccc ggcccatgga cgacctcggt gagcacaacg acgcagccag
     2161 cctgatggcg gatctgcgat ccagacaccc tgcattcact gcggcggtgc tggccgacac
     2221 cgtagtcacg gcccagcatc gcggcgagcg atacgagttc cgcagcaagg ccgacgccct
     2281 gctcgcggcc ctgcgcctgg cctgggtgag cgacgccttc ctggcccagg tcttttaccg
     2341 cgccaaggcc gcctgtcagc ggcgcgaggt gccggtcctg ccccgcctct tccaccggct
     2401 ttcgatgatg ctggcccagg tgtcgatcgg agatccggtg gtcatgcatc ccggtgtcta
     2461 catcgtccac gggcaggtcg ttctcgacgg gatcgtcgaa attcatcccg gggtcgtgat
     2521 ttctccctgg gtgacgatcg gcctgaaggc gggcaacgtg gtgggaccga cggtgaggga
     2581 gggagtgaac atcggcaccg gcgccaagct gatcggaccg atcgatgtcg ggaccggggc
     2641 aatgatcggg gcaaacgcgg tggtggtctc ggacgtgccc gaggggacca cggcggtcgg
     2701 ggtcccggcc gccaagtccg aatgaatccg gccatgcggg taagtactga acttcaacgg
     2761 aaatccgcgt gacactgcta gcgtccaccg ggttccagtt ggcagaaccg gagtcaacca
     2821 cctaaaggca ggtaattgat gg
//
LOCUS       DAOTIP010000208         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41817_length_2839_cov_3.015086, whole genome shotgun sequence.
ACCESSION   DAOTIP010000208 DAOTIP010000000
VERSION     DAOTIP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41817_length_2839_cov_3.015086"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="P5138_04890"
     CDS             <1..1474
                     /locus_tag="P5138_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRV59944.1"
                     /translation="GVVITWFVATVSIALLLDVPHPIAILIGATVIVSGPTVVIPILD
                     FIGPTQKVRRVLKWEGILIDPIGAIIAVVVFGSFDNSHGGRFDIAELFLSLGVGGVVG
                     VIGAALMMPILGTKKLAGRDKVAATLMMVVACFAAADAVLSDSGLVATIVMGVALANQ
                     HRVRIEQVAEFKETLVPILVGILFVLLAANVDIDQVADLGWKGLLVVAILIVVARPLA
                     ALTLVGLPFTWKEKVFFGSMAPRGIVAASTASAFGLALTDRDVPGAQYIIPVTFLIIA
                     GTVFYYSLITPWLARVLGLSGEHRPTLLMIGPPPWALTVGEGLTRAGAEVIVWTESPD
                     EAKAAADHGLVTFGGPLDPESKEADSPLEDVAAFAVISDDDSLNQLLSFQLAELYEAN
                     QVYRRRSPSDRAPIVNSEALELFHDGDVETDVQRRLAAGEPVSVVGPLAEVPDGAVVI
                     AAVRPKSGTMPAKIHLSTEEKPVVPDSNSTLLVLDPPVGV"
     gene            complement(1471..>2839)
                     /gene="zwf"
                     /locus_tag="P5138_04895"
     CDS             complement(1471..>2839)
                     /gene="zwf"
                     /locus_tag="P5138_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRV59945.1"
                     /translation="RRKLLPGLFHLDRAGHLSDCRVVGTSLEEMSNADFRRLAREACE
                     TFGRGRVNEEDWDRFERRLSWVDGGAGAIGLADAVAKAEADIGEERRRLHYLSVPPAA
                     AAAVIKTLGDAGLVERSRIIMEKPFGTDLATSQKLNAMVHEVFAEEQVFRIDHFLGKE
                     AALNILALRFANGLFEPIWSRNFIDHVQIDVPETLTVEGRGGFYDPTGAFRDMVVTHL
                     FQVLAFVAMEPPVELAPAAITVEKNKVFRSMKGIRPEDVVRGRYEGYRDEHGVAADSD
                     TETFIALRCRIDNWRWSGVPFFLRTGKAMAEGARIISIAFREPPKSMFPEGSGVGKKG
                     PDHLTFDLDESSRISLSYYGKRPGPGMLVDKLSMQFSLNEIESQGDTLEAYERLIRDA
                     MGGDHTLFTTAEGIEELWNTAAPMLEDPPEAMIYERGSWGPRQVHDLIAPRRWRLPFQ
                     RRWRG"
BASE COUNT          431 a          950 c          932 g          526 t
ORIGIN      
        1 cggggtggtc atcacctggt tcgtggcgac ggtttccatc gcccttctgc tggacgttcc
       61 gcatccgatc gcgatcctga tcggcgccac ggtgatcgtt tccggcccga ccgtggtcat
      121 cccgatcctc gacttcattg gcccgaccca gaaggtccgc cgggtcctga agtgggaggg
      181 aatcctgatt gacccgatcg gggcgattat tgcggtcgtc gttttcgggt ccttcgacaa
      241 ttcccacggc ggcaggttcg acatcgccga gctgttcctc tcgctcgggg tcggcggcgt
      301 ggtcggggtg atcggtgcgg ccctgatgat gcccatcctc ggcaccaaga aactggccgg
      361 ccgcgacaag gtcgcggcca ccctgatgat ggtcgtcgcc tgcttcgcgg ctgccgatgc
      421 ggttctttcc gactcggggc tggtcgcgac gatcgttatg ggggtcgccc tggccaatca
      481 gcacagggtc aggatcgagc aggtcgccga gttcaaggag actctggtgc cgatcctggt
      541 cggcattctc ttcgtcctgc tggctgcgaa cgtggacatc gaccaggtgg ccgacctggg
      601 ctggaagggg ctgctggtgg tcgcgatact gatcgtcgtc gcccgccccc tggcggcgct
      661 gacgcttgtc gggctgccct tcacctggaa ggaaaaggtg ttcttcggct cgatggcgcc
      721 ccggggcatt gtcgccgcgt cgactgcctc cgccttcggc ctcgccctga ccgatcgcga
      781 cgtcccgggc gcccagtaca tcatcccggt caccttcctg atcatcgccg gcacggtctt
      841 ctactactcg ctgatcacgc cctggctggc ccgggtgctg ggactctcgg gggagcaccg
      901 gccgaccctg ctgatgatcg ggccgccccc ctgggccctc acggtcggcg aaggcctgac
      961 ccgggccggg gctgaagtga tcgtctggac cgagtcaccc gatgaggcga aggcagcagc
     1021 cgatcacggg ctggtcacct tcggggggcc cctggacccc gagagcaagg aagccgattc
     1081 cccgcttgag gacgtcgccg cgttcgccgt gatcagtgac gacgacagcc tcaaccagct
     1141 gttgtccttc cagctcgccg agctctacga ggccaaccag gtctaccgcc ggcgctctcc
     1201 ttcggaccgg gcgccgatcg tgaattcgga agcactcgag ctcttccacg acggggacgt
     1261 cgagaccgat gtccagaggc ggctcgcggc cggggagccc gtctccgtgg tcgggccgct
     1321 tgccgaggtg cccgacgggg cggtggttat cgccgcggta cgccccaagt cgggaacgat
     1381 gccggcgaag attcatctct cgaccgagga aaagccagtg gtgcccgaca gcaactccac
     1441 cctgctggtc ctggatcccc ccgtcggggt ctagcctctc cagcgcctct ggaatggcag
     1501 gcgccagcgg cgcggggcga tcaggtcatg gacctgtctg ggcccccagg agccgcgctc
     1561 gtagatcatc gcctcgggcg gatcctcgag catgggggcg gccgtgttcc agagctcctc
     1621 gatgccttcg gcagtggtga agagcgtgtg gtcgcctccc atcgcgtccc ggatcagcct
     1681 ctcgtaggcc tccagcgtgt cgccctggct ctcgatctcg ttgagcgaga actgcatgct
     1741 cagcttgtcg accagcatgc ccggtcccgg tcgcttgccg tagtaggaaa gcgagatccg
     1801 ggaagactca tcgaggtcga aggtgagatg gtccggcccc ttcttgccga ctccggaacc
     1861 ctcggggaac atgctcttgg gcggctcccg gaaggcgatc gagatgattc tggctccctc
     1921 ggccattgcc ttgccggtcc tgaggaagaa aggcacgccg gaccagcgcc agttgtcgat
     1981 ccggcacctc agcgcgatga aggtctcggt gtcggaatcg gccgcgactc catgctcgtc
     2041 ccggtagccc tcgtatcggc cccggaccac gtcctccggc cggattcctt tcatcgatcg
     2101 gaacaccttg ttcttctcga ccgtgatcgc cgccggggcg agctcgaccg gcggctccat
     2161 cgcaacgaac gccagcacct ggaagagatg ggtgaccacc atgtcacgga aggccccggt
     2221 cgggtcgtag aagccgccgc ggccttcgac ggtcagggtc tccggcacgt cgatctgcac
     2281 atgatcgatg aaattgcgac tccagatcgg ctcgaacagg ccgttcgcga atcgaagggc
     2341 gaggatgttc agggcggctt ccttgccgag gaagtggtcg atccggaaca cctgttcctc
     2401 ggcgaagact tcatggacca tcgcattgag cttctgggag gtggcgaggt cggtcccgaa
     2461 cggcttctcc atgatgatcc gcgaacgttc gaccagtccc gcgtcaccga gggtcttgat
     2521 caccgcggcc gcggcggccg gcggcacgct gagatagtgg agccggcggc gctcctcgcc
     2581 gatgtccgcc tccgctttcg ccacggcgtc ggcgaggccg atcgcgccgg cacctccgtc
     2641 cacccaggaa agccggcgtt cgaaacggtc ccagtcctct tcgttcaccc ggccccggcc
     2701 gaaggtctcg caggcttccc gcgccagtcg gcggaaatcg gcgttcgaca tttcttcgag
     2761 cgaggtgccc acgacccggc agtcggaaag gtgtccggcc cggtccagat gaaaaagccc
     2821 gggcaggagc ttccggcgg
//
LOCUS       DAOTIP010000209         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41853_length_2837_cov_2.990295, whole genome shotgun sequence.
ACCESSION   DAOTIP010000209 DAOTIP010000000
VERSION     DAOTIP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41853_length_2837_cov_2.990295"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="P5138_04900"
     CDS             complement(<1..423)
                     /locus_tag="P5138_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRV59946.1"
                     /translation="MNGEEVTDEDLDLTPYVCDFEEGDKVVYPHHGAGVVLKKADAEL
                     MGETREYLTIKILHNDLTVKVPTAKAGEAGLRRVIDEDEIKKVISVLSDEVSDMPKNW
                     NRRFKYNKEKIKTGDVFELAEVVRNLALREMEKGLSTGE"
     gene            complement(483..1595)
                     /gene="disA"
                     /locus_tag="P5138_04905"
     CDS             complement(483..1595)
                     /gene="disA"
                     /locus_tag="P5138_04905"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HRV59947.1"
                     /translation="MSPAVESDLAHLSERQDPRLLRAIDAVAPGTELREGIDNILHAR
                     TGGLIVVGETEDFAFMLSGGIKLDIDYTPAMLYQVAKMDGAILVNADGSKITWANVQM
                     MPDPTIHSSETGTRHRTAERISKQVDALVIAISERREVVSLYLEGVKYILQDIPAVLA
                     KSNQALATLTKYRTRLDELSSRLTRAEFVGGAVLYDALMVLQRSELVSRMATEVERYV
                     IELGTEGRLIEMQLEETMVGVASERLALMRDYAVEDTEENVQFMLEELAALPHQDVLD
                     FGRLAELLGYDRKVNTLDLATEPRGYRMLGHVPRLPRLVVQKIVMNFGSVEAALAASD
                     EELAAVDGVGPARAKEIREGVRRLQESVMADRFLNT"
     gene            complement(1719..>2837)
                     /gene="radA"
                     /locus_tag="P5138_04910"
     CDS             complement(1719..>2837)
                     /gene="radA"
                     /locus_tag="P5138_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRV59948.1"
                     /translation="VLLGGSPGIGKSTLTGMAMANLQAGGIETLYVSGEESAAQVRMR
                     AERLGPAALEVKVLAESSLEVVLGTIESERPAVCVIDSIQTMHSSELSGAPGSVGQVR
                     EAANALAEQAKRLEVAVILVGHVTKEGSLAGPRVLEHLVDCVLRFEGERERTWRTLRA
                     VKNRFGATSEVGVFEMRSGGLTEVLDPSARFVEEATGAQGSVVLASMEGTRPVLVEVQ
                     ALVSPSELEIPRRVVNGIDRNRLSMILAVLARHARLGLGTADVFVNVVGGVRIDEPGA
                     DLAVALAVASAQRGETLSGPDGRPLACFGELGLTGDLRYVAHADRRVAEAAKFGLEPV
                     IGPRGGERLSGLAPCATVKQALKAAFSGSSADSRAAAA"
BASE COUNT          468 a          976 c          851 g          542 t
ORIGIN      
        1 ctcaccggtc gagaggccct tctccatctc gcgaagcgcg aggttgcgta ccacctcggc
       61 cagctcgaag acgtcacccg tcttgatctt ttccttgttg tacttgaacc ggcggttcca
      121 gttcttcggc atgtcggaaa cctcgtccga cagcaccgaa atgaccttct tgatttcgtc
      181 ctcgtcgatc acccggcgca gcccggcttc gccggccttc gcggtcggga ccttgacggt
      241 caggtcgttg tgcaggatct tgatcgtcag gtactcgcgg gtctcgccca tgagttcggc
      301 atcggccttc ttcagaacaa cgcctgctcc gtggtgcgga tagacgacct tgtcgccttc
      361 ctcgaaatcg cacacgtacg gagtcagatc gagatcttca tcggtcactt cttcgccgtt
      421 cactgcctct gaatcggaaa tcagatgctc ctttgtctct tagctgacga ctgaatgcgt
      481 cgtcatgtgt tcaaaaaccg gtctgccatc acggactcct gaagcctccg cactccttcg
      541 cggatctcct tggcccgggc cgggccaacg ccgtcgacgg cggcaagttc ctcgtcgctg
      601 gccgcgagcg cggcttcgac gctgccaaag ttcatgacga tcttctggac cacgagccgc
      661 ggcaggcgcg gaacgtggcc gagcatcctg tagccgcgcg gttcggtggc caggtcaagc
      721 gtgttgacct tgcggtcata gccgagcagt tcggccaggc ggccgaagtc gagcacgtcc
      781 tgatgcggca gggcggccag ttcctcgagc atgaactgga cgttctcctc ggtgtcctca
      841 accgcgtaat cgcgcatcag tgcgagccgc tccgaagcaa ccccgaccat ggtctcctcg
      901 agctgcattt cgatcagcct gccttcggtg ccgagctcga tcacgtatcg ctcgacctcg
      961 gtcgccatcc gggaaaccag ctccgagcgc tgcagcacca tcagtgcgtc gtagaggacg
     1021 gcgccgccga cgaattcggc ccgggtcagg cggctcgaga gctcgtcgag ccgggtccgg
     1081 tacttggtca gcgtggcgag ggcctgattg gacttggcca gcaccgcggg aatgtcctga
     1141 aggatgtact tgaccccttc gagatagagc gagaccactt cccgccgttc cgagatcgcg
     1201 atgaccaggg catcgacctg cttcgagatc cgttcggcgg tgcgatggcg ggtgccggtc
     1261 tccgaggagt ggatggtcgg gtcgggcatc atctggacgt tggcccaggt gatcttgctg
     1321 ccgtcggcgt tgaccaggat cgccccgtcc atcttcgcca cctggtagag catcgccggc
     1381 gtgtagtcga tgtccagctt gatgccaccg gaaagcatga aggcgaagtc ctcggtttcc
     1441 ccgaccacga tcagaccgcc ggtacgggcg tggaggatgt tgtcgatccc ttccctcagc
     1501 tcggtgccgg gggcgacggc gtcaatcgcg cgcagcagtc tgggatcctg acgttcagac
     1561 agatgtgcca gatcactctc taccgctggt gacattcaga gaattatgcc agattcggct
     1621 ttctaatgcg gtctggcgtg gttccgggcg ctcttttgcg ggggttgtga ccggccctca
     1681 atccgctccg gaaagccgaa tttagcctcg cctgaagatc aggctgcggc ggcccgggaa
     1741 tcggctgaac ttccggaaaa tgcggctttc agggcctgct tcacggtggc gcagggagcg
     1801 agccccgata gccgctctcc accccttggg ccgatcaccg gttcgaggcc gaatttcgct
     1861 gcctcggcga ctctgcggtc ggcatgggcg acgtacctca ggtccccggt caggcccagt
     1921 tcaccgaaac aggcgagcgg acggccatca ggcccggaaa gggtctcgcc gcgctgggcc
     1981 gaagcgaccg cgagcgccac ggcgagatcg gccccgggct catcgatcct caccccgccg
     2041 accacgttca caaagacgtc ggccgtaccg agacccagcc tcgcgtgccg ggccagcacc
     2101 gcaaggatca tgctgaggcg gttgcggtca atgccgttga cgacccttcg cggaatctcg
     2161 agttcactcg gggagaccag agcctgcacc tcgaccagca ccggtctggt cccttccatc
     2221 gaggccagaa ccaccgaccc ctgggccccg gtcgcttcct cgacgaagcg ggccgacgga
     2281 tccagcactt cggtcaggcc tcccgagcgc atctcgaaca ccccgacctc cgaggtcgcc
     2341 ccgaacctgt tcttaaccgc ccgcagggtg cgccatgtac gttcccgttc gccctcgaag
     2401 cgaagcacgc agtcgaccag gtgttccagc accctcggcc ccgccaggct gccttccttg
     2461 gtcacgtggc cgaccaggat caccgccact tcgagccgct tggcctgctc ggcgagggcg
     2521 ttggccgcct ccctgacctg cccgaccgaa ccgggagcgc cgctcaattc cgaactgtgc
     2581 atggtctgga tcgaatcgat cacgcagacc gccggccgct ccgactcgat cgttcccagc
     2641 accacctcca gcgagctctc ggccagcacc ttgacttcca gggccgccgg cccgagccgc
     2701 tcggcccgca tccgcacctg cgccgccgat tcctcaccgg agacatagag cgtttcgatt
     2761 cccccggcct ggaggttcgc catcgccatc ccggtcaggg tgctcttgcc gattcccggc
     2821 gagcccccga gcaacac
//
LOCUS       DAOTIP010000210         2834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41923_length_2834_cov_3.261965, whole genome shotgun sequence.
ACCESSION   DAOTIP010000210 DAOTIP010000000
VERSION     DAOTIP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41923_length_2834_cov_3.261965"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="speB"
                     /locus_tag="P5138_04915"
     CDS             <1..942
                     /gene="speB"
                     /locus_tag="P5138_04915"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRV59949.1"
                     /translation="TMWSSLLHAGLSNSFLRLPHVPADAEALREHGAKAAIYGIPFDA
                     TNISRTGANYGPRGIRDVSCQFLTFNATLGFDLLEALNPVDAGDTDIVLANAEKTFAR
                     AQSDLGEIIASGALPVTLGGDHSITIPAARAIVDAHDDPGLVLIDMHLDTAPDVGGEL
                     LNHCCPITRAVDAGFDPQKIALVGISGWMNPKTELQYCLDHGITVIWLEEIWEKGTRW
                     AADKAREVAGAGDGIYLSFDIDSLDAAHAPGTCCPSPGGLTSREGIELVRGVSSGGLL
                     GVDVVEVAPSLDSTPATALIGGRIALEAMAFHAGATL"
     gene            946..1737
                     /locus_tag="P5138_04920"
     CDS             946..1737
                     /locus_tag="P5138_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRV59950.1"
                     /translation="MRVALAQSDPVGHAKRDCEAACSVIEALTDADLVLFPELFVGGY
                     NTDDPGSVAIEVEDPALATVFEACRRSGCAAVIGFTELLEDGGFANSAVCVQSDGIPA
                     GVYRKTHLFGPAERSAFVAGDQLRVFTLAGRAIGPEICFDVEFPEPARLMATAGAEIL
                     VTISANMEPYAADHRLAARARALDNRLHHLYVNRTGRESGHTFTGESCVINPSGTVLA
                     ELGRGEGVLECVISPSQPDPDTSYLDQIRADLKVIVQHQVNGGDS"
     gene            1734..>2834
                     /locus_tag="P5138_04925"
     CDS             1734..>2834
                     /locus_tag="P5138_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59951.1"
                     /translation="MSTQSEGSPALFTRKATGLVREAKTRDALFYNLLWSSVALTFAF
                     YWLFFAFYPGANPYVGILIAAGLGLPGSFLYAMLTQIMPRTGGDYVFNSRALHPSIGF
                     AGNFSYCFWLAIVIGVYTTYFAAYGMGAWGRMMAGFTGSSGWMSFGDWFSTEWGLFIT
                     GTVALLLSAALFIRGGTRLFFKVQAVGFFIYLAGAFLVPILVGLFQSESGFVSAFNDY
                     AANLGVNDAVGALTKSASDAGYASSSFDFEMTVKSVSIFWFIFGFIYSSNYFAGEIRV
                     TRGTHLKSMPGAVLIAVTALLLLLPAFLNVAGYDFNGMLGYADPASYGFVAGAPAYPE
                     LMAIASNSPVLGTIIILGFALGLLLWLPQTLLL"
BASE COUNT          484 a          941 c          845 g          564 t
ORIGIN      
        1 accatgtgga gcagcctgct tcatgccggt ctcagcaatt cattcctccg gcttcctcat
       61 gtaccagccg atgccgaagc gctcagggaa cacggagcca aggccgcgat ctacggaata
      121 ccgttcgacg caacgaacat cagccggacc ggggccaact acgggccccg gggcatcagg
      181 gatgtctcct gccagtttct tacgttcaac gcaacgctcg gctttgacct gctcgaggcg
      241 ctgaatccgg tcgacgccgg ggacacggac atcgtgcttg ccaacgccga gaagactttc
      301 gctcgggccc aatccgacct gggcgagatc attgcctcag gggcactgcc agtcacactc
      361 ggtggcgatc actcaataac cattccggcc gcacgcgcaa tcgtcgacgc ccacgatgat
      421 ccgggactgg tcctgatcga catgcatctc gacacggcgc ccgacgtggg cggcgagctg
      481 ctcaaccact gttgcccgat cacccgagcg gttgatgccg gtttcgaccc tcagaagatc
      541 gccctggtcg gaatctccgg ctggatgaat ccgaagacgg aactccagta ctgccttgac
      601 cacggaataa ccgtcatctg gcttgaggaa atctgggaga agggcacccg ctgggcggcc
      661 gacaaggcgc gcgaagtggc gggagccgga gatggcatat atctctcctt cgacatcgac
      721 tcgcttgacg cggcacatgc cccgggaacc tgttgcccca gcccgggagg actgaccagc
      781 cgcgagggga tcgaactggt ccgcggcgtc tcttccggcg gcctcctcgg agtcgacgta
      841 gtcgaggttg cgccaagcct ggactccacc ccggcgaccg cgttgatcgg cggacgcatt
      901 gccctggaag cgatggcgtt tcatgccggg gcgacgctct aggcgatgag agtcgctctc
      961 gcccagtcgg acccggtggg tcatgcgaag cgagactgcg aggccgcctg ctcggtcatc
     1021 gaagccctga ccgatgctga cctcgtgctg tttcccgaat tgttcgtcgg cgggtacaac
     1081 acggacgatc cgggatcggt cgctatcgag gtggaagatc ccgctctggc cacggtcttt
     1141 gaggcctgcc gccgatccgg ctgcgccgcc gtcatcggct tcaccgaact gctcgaggac
     1201 ggtggcttcg caaactcggc cgtctgtgtt cagtctgacg ggatacctgc cggggtctat
     1261 cgcaagaccc acctgttcgg cccggcggag cgttcggcgt ttgtcgccgg agaccagctt
     1321 cgggtcttca ctcttgccgg acgggcaata ggtccggaga tctgttttga cgtcgagttc
     1381 cccgagccgg cccggctcat ggccacggcc ggggcggaaa tcctggtgac gatctcggcc
     1441 aacatggagc cgtacgccgc cgatcaccga ctggcggcca gggcccgtgc cctcgacaac
     1501 cggcttcacc acctctacgt aaacagaacg ggcagggaat ccggccacac cttcaccggg
     1561 gagtcctgcg taatcaaccc gtccggaacc gtcctggctg aactgggccg gggcgaaggc
     1621 gtcctcgaat gcgtcatttc accgtcgcaa ccggacccgg atacgtccta tctcgaccag
     1681 atccgagccg acctgaaggt aatcgtccaa caccaagtga acggaggaga ttcatgagta
     1741 cccaaagcga agggtcaccg gcccttttca cccgcaaggc aacgggcctg gtccgggaag
     1801 caaagaccag ggacgccctc ttctacaacc tgctctggtc gagcgtcgcg ctcaccttcg
     1861 cgttctactg gctcttcttt gccttctatc cgggagcgaa cccctacgtg gggatcctta
     1921 tcgcggccgg tctgggtctg cccgggtcgt tcctctacgc gatgctgacg cagatcatgc
     1981 cgcggaccgg tggcgattac gtcttcaaca gccgggcact tcaccccagc atcggattcg
     2041 ccggaaactt cagctactgc ttctggctgg ccatcgtgat cggcgtttac accacgtact
     2101 tcgctgccta cgggatgggc gcctggggcc gcatgatggc cggtttcacc ggatcgagcg
     2161 gctggatgag cttcggcgac tggttctcga ccgagtgggg cctcttcatc acgggaacgg
     2221 tcgcgcttct gctttcggcg gccctgttca tccggggcgg cacccgcctc ttcttcaagg
     2281 tccaggcggt cgggttcttc atctaccttg ccggcgcctt cctggtgccg attctcgtcg
     2341 gtctctttca gagtgaatcc ggctttgtct cggccttcaa cgattacgcc gccaacctcg
     2401 gcgtgaacga cgcggtcggg gccctgacca agtccgcaag tgacgccggc tacgcctctt
     2461 ccagctttga tttcgagatg accgtcaagt ccgtttcgat cttctggttc atcttcggct
     2521 tcatctattc gagcaactac ttcgccggag agatcagggt cacccggggc acccatctga
     2581 agtccatgcc cggcgcggtc ctgatcgccg taaccgccct gctgctcctg ctgcccgctt
     2641 tcctgaacgt cgccggttac gacttcaacg ggatgctcgg ctacgcggac ccggcttcat
     2701 acggcttcgt cgcgggagca ccggcttacc ccgaactgat ggcgatcgct tcgaacagcc
     2761 cggtgctcgg aacgatcatc atcctgggct tcgctctcgg cctcctgctg tggctccccc
     2821 agactctgct gctg
//
LOCUS       DAOTIP010000211         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41946_length_2833_cov_3.239741, whole genome shotgun sequence.
ACCESSION   DAOTIP010000211 DAOTIP010000000
VERSION     DAOTIP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41946_length_2833_cov_3.239741"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1419
                     /gene="tig"
                     /locus_tag="P5138_04930"
     CDS             19..1419
                     /gene="tig"
                     /locus_tag="P5138_04930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRV59952.1"
                     /translation="MKTTVTELEDSRARVEVEVPADEVSVQVQRAARALAREMRMPGF
                     RKGKAPPSLAIQRLGFDTVFQEAIQEALPEWYQRSVYASGIVPIGSPDVEITKAPSND
                     GETVEFKYEVGVRPTAELGEYKGLEVEKAGIEVPDEEIDREVDRMREGMASLDVVDRA
                     AEEGDHVLVDFVGSLDGVEFEGGSATDHTIEVGSGQLIDDFEEQVIGAKAGDEVAVNV
                     NFPEDYGAAELAGQNADFAVTVKEVRVKQMPEADDDFASDASEFDTIEELRADIAERL
                     GESVGERIEQDFRVAAVDAAVANAKVGIPEDILRARGEERWDRVERQMAQQGMNPDTY
                     LQMQGKTRDEIIDESLDDADQEIRREAVLVAIADAEGIEVSDEEMAEELEHAASHERT
                     TGAKLLERLKRDGRDKMVAADIEVRKAMDVIADSANAVEMSAKDAAKKLWGLEDEEEE
                     DSSDSEAGPEEISPDE"
     gene            1459..2043
                     /gene="clpP"
                     /locus_tag="P5138_04935"
     CDS             1459..2043
                     /gene="clpP"
                     /locus_tag="P5138_04935"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRV59953.1"
                     /translation="MSPLVPMVVEQTSRGERSFDIYSRLLNERIIFLGTGVNEDIANL
                     VVAQLLHLDAEDPEKDISLYINSPGGSVYAGLAIYDTMQFIKPDVQTICVGIAMSMGA
                     LLLAGGAEGKRMSLPNSKILIHQVSSGFQGQATDIEIHANEIIDTRSRLDKILAKHTG
                     QDLEKITHDTERDYFMSADEAATYGLIDRVIEHR"
     gene            2171..>2833
                     /locus_tag="P5138_04940"
     CDS             2171..>2833
                     /locus_tag="P5138_04940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV59954.1"
                     /translation="MRARAVTPKEAMPFSVDPIRFHFRFSANRRVRVAIRINRFVAGG
                     ERFVRRIVTGKLAPKRWHHIYWDGRTRGGRLAPAGRYRVMAGPVGGPFAVLGRIRLHS
                     HEFPVDGPHGTRGYIGEFGAPRTGGRTHEGFDITAACGTPLVAVRAGTILKKAFDPVL
                     YGNYVVLKGEGERRTYKYAHLIRPAPVYKGQKVRAGRRLGSVGQTGNAASTPCHLHIE
                     IRT"
BASE COUNT          549 a          928 c          914 g          442 t
ORIGIN      
        1 cgctaggctc attcgacctt gaagaccacc gttactgaac tagaagattc gagggcacga
       61 gtcgaggtcg aggttccggc cgacgaagtg tccgttcagg tccagcgtgc cgcccgggcg
      121 ctggcccgtg aaatgcgcat gcctggcttc cgcaagggca aggcgccacc ctcgctagca
      181 atccagcggc tcggcttcga caccgtcttc caggaggcga tccaggaggc cctgcccgag
      241 tggtaccaga ggtcggtcta tgcatccggg attgttccga tcgggtcgcc cgacgtcgag
      301 atcaccaagg cgccgtccaa tgacggcgag acggttgagt tcaagtatga ggtcggcgtt
      361 cgcccgaccg ccgagctcgg cgagtacaag ggtctcgagg tcgagaaggc aggcattgaa
      421 gttcccgacg aagagatcga ccgcgaggtc gaccgcatgc gcgagggcat ggccagtctc
      481 gatgtggtcg accgcgccgc cgaggaaggc gaccacgtgc tggtcgactt cgtcggttcg
      541 ctcgacgggg tcgagttcga aggcggctcc gccaccgatc acacgatcga ggtcggctcc
      601 ggtcagctga tcgacgactt cgaggagcag gtcatcggcg ccaaggccgg cgacgaggtc
      661 gcagtgaacg tgaacttccc ggaggactac ggagccgccg agctggccgg tcagaacgcc
      721 gatttcgcgg tcaccgtcaa ggaagtccgg gtcaagcaga tgccggaagc cgacgacgac
      781 ttcgcttccg acgcttccga gttcgacacg atcgaggagc tccgcgccga catcgccgag
      841 cggctcggcg agtccgtcgg ggaacgaatc gagcaggact tccgggtagc cgccgtcgat
      901 gcggccgtgg ccaacgccaa ggtcggcatc cccgaggaca tcctcagggc ccggggcgag
      961 gagcgctggg accgggttga gcggcagatg gcccagcagg gcatgaatcc cgacacctac
     1021 cttcagatgc agggcaagac ccgggacgag atcatcgacg agtcgctcga tgatgctgac
     1081 caggagattc gccgcgaggc cgtgctggtc gccatcgccg acgccgaggg catcgaggtc
     1141 agcgacgagg agatggccga ggaactcgag cacgccgctt cgcatgagcg caccaccggc
     1201 gccaagctgc tcgaacgcct caagcgcgat ggccgcgaca aaatggtcgc cgccgacatc
     1261 gaggtccgca aggcaatgga cgtgatcgcc gactctgcca acgcggtcga gatgtcagcg
     1321 aaggacgcgg caaagaagct ctggggcctc gaagacgagg aggaagaaga ttcctccgac
     1381 agcgaagccg gccccgaaga gatatccccg gacgaatagt ccgtcggccc aaccacaagc
     1441 ctctatagga ttgcccgcat gagtccactt gtccccatgg tcgtcgagca gacctctcgc
     1501 ggagagcgtt cgttcgacat ctattcccgg ctcctcaacg agcgcatcat cttcctcggc
     1561 accggcgtga acgaggacat cgccaacctg gtggtggccc agctgcttca cctcgatgcg
     1621 gaggatcccg agaaggacat cagcctctac atcaactcgc ctggcggctc cgtttacgcg
     1681 ggcctcgcca tctacgacac gatgcagttc atcaagcccg acgtccagac catctgtgtc
     1741 ggcatcgcga tgagcatggg cgccctgctg ctggcaggcg gcgccgaagg caagcggatg
     1801 tccctgccga actcgaagat cctgatccat caggtctcct ccggattcca gggtcaggcg
     1861 accgacatcg agatccacgc caacgagatc atcgacaccc gcagccgcct cgacaagatc
     1921 ctcgccaagc acaccggcca ggacctcgag aagatcaccc acgacaccga gcgcgactac
     1981 ttcatgagcg ctgacgaggc cgccacctac gggttgatcg accgggtcat cgagcatcga
     2041 taggcggacc atcaccagtt tcaccggagc cgtcgccatc ctggcggcgg ctcttgtgtt
     2101 tgtcggttcg gcaccgggcg cgaagccgaa aacgccgacc ggtccttctc ccgcgataac
     2161 ggcatttcag atgcgggccc gcgcagttac cccgaaggaa gcgatgccgt tttcggtcga
     2221 cccgatccgc ttccacttcc gcttttcggc gaaccggcgt gtccgtgtcg cgatccggat
     2281 caaccgcttt gtggccggcg gggaacggtt cgtgcgccgg atagtcaccg gaaagctggc
     2341 gccaaagcgt tggcatcaca tttactggga cggccggacc cggggcggcc ggctcgcgcc
     2401 ggcgggcagg taccgggtga tggcggggcc ggtcggcggt cccttcgccg tccttggccg
     2461 aatccggctc cactcccacg agttcccggt cgacgggcca cacggcaccc ggggctacat
     2521 cggcgagttc ggggcgccac ggacaggtgg ccgcactcac gagggctttg acatcactgc
     2581 cgcctgcgga actccgctgg tcgcggtccg ggccggaacc atcctcaaga aggcctttga
     2641 cccagtgctc tatggcaact acgtagtgct caagggcgaa ggggagaggc gtacctacaa
     2701 gtacgcacat ctgatccgtc cggccccggt ctacaagggg cagaaggtcc gggcgggccg
     2761 gcgcctcggt tccgtgggcc agaccggcaa cgcggcctca accccctgcc acctccacat
     2821 cgagatacgg acc
//
LOCUS       DAOTIP010000212         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_41955_length_2833_cov_2.383729, whole genome shotgun sequence.
ACCESSION   DAOTIP010000212 DAOTIP010000000
VERSION     DAOTIP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41955_length_2833_cov_2.383729"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5138_04945"
     CDS             complement(<1..667)
                     /locus_tag="P5138_04945"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HRV59955.1"
                     /translation="MGRKTQVAIVAIVVLLLGGAVAAYAYDGAQKDTIADGVTVAGVD
                     LSGMNSEEATQALTNQVLAPQRKPVTVTFRKQKFRLPAKQLKIRANVQQAVDQALEES
                     RSGSLPTRVFREITGGEVQATVPVKVSYSQKAVNQFVKKVADGIVQEPVDASVAAGPD
                     SLSVVKSKNGYKLRDNLLTEQLNKLLDSGQGTRTLVAKVNVTKPSVTTAEVAEKYPTY
                     LTLD"
     gene            complement(776..1903)
                     /locus_tag="P5138_04950"
     CDS             complement(776..1903)
                     /locus_tag="P5138_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV59956.1"
                     /translation="MADDPKGARRVLVTGLSTYWGGRLAAALESNPGIEAIIGVDSRE
                     PTRELARTEYVKVGAEHRLIEKIVRAARIDTVVDTRLMVDSVRGPDGRRPEQIHENNV
                     IGTLNILAACSASGSPVRKLVFKSSAHWYGCAQDDPAYFTEEMSRRYEPETRVERDVV
                     EAEAAVAEFRDQRPNVTVSVLRFANVLGAEVDTSHVQLFGMPAVPMIAGFDPRYQFVD
                     EIDVVHALEHVVVRDLPGIYNVAGDGVLTLSEVISLLGKRPLPVIPPWGTSIAMAGFR
                     RIGLTLPSEMVKQLRFGRGIDNRSFKATGFHFRYTSRETVLHLRDRLRLDPVAERRET
                     EYSYEPAVEDFLRHSPHVDRSRKDMGREDAALFDPSPPVQD"
     gene            1957..>2833
                     /locus_tag="P5138_04955"
     CDS             1957..>2833
                     /locus_tag="P5138_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV59957.1"
                     /translation="MTTEAPDNDGLLTGTPPPAPVHDDYGVSDGRWLKIDWSERIRQV
                     TVHSALGETPISYVDQGEGPPILLVHGLGGSWRNWLENIPFLSDSHRVVALDLPGFGL
                     SPMPHEPISMPAYGDLVLRFADAVGLGPETFLIGHSMGGFISTEAVTCDPDRFARLSL
                     VSAAGISFATISGPRKSVTNVILKAMLPVLANRAERNMSRKRLRTASFAGVIAHPSMI
                     SPKILWELGTYGALHAPALLPAAWALGGYDNRPSLSHIEIPTQIVWGQQDRLVPVAAA
                     YSYSRRIEHAELSLVD"
BASE COUNT          483 a          929 c          901 g          520 t
ORIGIN      
        1 gatcaagggt gagataggtc ggatacttct cggcgacctc ggccgtggtg accgacggct
       61 tggtcacgtt gaccttggcg acgagcgtcc tggtgccctg gccgctgtcg aggagcttgt
      121 tcagctgctc ggtcagcagg ttgtcccgca gcttgtagcc gttcttggac ttgacgacac
      181 tcagcgaatc gggtccggcg gcaaccgagg cgtcaaccgg ttcctggacg atgccgtcgg
      241 caaccttctt cacgaactgg ttcacggctt tctgcgagta gctgaccttc accggaacgg
      301 ttgcctggac ctcgccaccg gtgatctccc ggaagacgcg ggtcggcagc gagcccgagc
      361 gactctcttc gagagcctga tcaacagcct gctggacgtt ggcgcgaatc ttcagctgtt
      421 tggccggcag ccggaacttc tgcttcctga aggtcacggt gaccggcttg cgctggggag
      481 cgagcacctg gttggtcagc gcctgggtcg cttcctcact gttcatgccg ctcagatcga
      541 ccccggcgac ggtcaccccg tcggcgatgg tgtccttctg ggccccgtca tacgcgtagg
      601 cggcaaccgc ccctcccaga aggaggacga cgatcgcaac tatggctacc tgagtcttgc
      661 ggcccaatgg attcctgctc tctggaattc gtcgaaagtc ctctacgaat tgtagatacc
      721 acgacgggcg ggcatttccc tccagatgag agaaatcgac tttgcaggcg gggaatcagt
      781 cctggaccgg aggcgaaggg tcaaacaggg ccgcatcctc ccgtcccatg tccttgcgcg
      841 agcggtccac gtgagggctg tgacgaagga aatcctcgac cgccggctcg tagctgtact
      901 cggtttccct cctctccgcc accgggtcaa gccggagacg gtcccgcaga tgaaggaccg
      961 tttccctcga ggtgtaccgg aagtgaaaac cagtggcctt gaaggaccgg ttgtcgattc
     1021 cccgtccgaa acgcaactgc ttgaccatct cggacggcag ggtgagcccg atccggcgga
     1081 agccggccat cgctatcgaa gtcccccagg gcgggatcac cggcagcgga cgtttcccca
     1141 gcagcgagat cacttccgac agggtcagga ccccgtcacc ggccacgttg tagatgcccg
     1201 gaagatcacg aaccaccaca tgctcgagcg catggaccac gtcgatttcg tcgacgaact
     1261 ggtagcgggg atcgaagccg gcgatcatcg gcaccgccgg catgccgaag agctggacat
     1321 gcgaagtgtc gacctcggca ccgaggacat tggcgaaccg gagcaccgag acagtcacat
     1381 tggggcgctg gtcccggaac tcggccaccg ccgcctcggc ctcgaccacg tcgcgttcca
     1441 cccttgtctc gggttcgtag cgccgggaca tctcttcggt gaaataggcc gggtcgtcct
     1501 gggcgcagcc ataccagtgg gccgagctct tgaagaccag ctttcgcacg ggcgagcccg
     1561 aagccgagca ggcagccagg atgttgagcg tgccgattac gttgttctcg tggatctgct
     1621 cggggcgccg gccgtcgggg ccccggaccg agtcgaccat gaggcgggtg tccaccacgg
     1681 tgtcgatccg ggccgcccgg acgatcttct cgatcagccg gtgctcggct cccactttca
     1741 cgtattcggt gcgggcgagc tcgcgggtcg gttcgcggga atcgaccccg atgatcgcct
     1801 cgatccccgg attgctctcc agggcagcgg cgagcctccc accccagtag gtggagagcc
     1861 ccgtgaccag cacccggcgt gcacctttcg ggtcgtccgc catcgacctc attgtttcag
     1921 gcgggacggg gttcgaggat gtatagagtc gttcatgtga ccacggaagc gcccgataat
     1981 gacggtctcc tgaccgggac tcctcccccc gccccggtcc acgacgacta cggggtttcc
     2041 gacggacgct ggctgaagat cgactggtcc gagaggatcc ggcaggtcac ggttcattcg
     2101 gcgctcggag aaaccccgat cagctacgtg gatcagggag aaggcccgcc gatcctgctc
     2161 gttcacgggc tgggcggaag ctggcgcaac tggctggaga acatcccgtt cctgtcggac
     2221 agccatcgcg tggtggcgct cgacctgccc ggcttcggcc tcagcccgat gccccatgag
     2281 ccgatctcga tgccggccta tggtgacctc gtgctccggt tcgccgatgc ggtcggcctc
     2341 gggccggaga ccttcctgat cggccattcg atgggcgggt tcatttcgac cgaggcggtc
     2401 acctgcgacc ccgaccgctt tgcgcggctg agtctcgtct cggccgcggg gatcagtttc
     2461 gcgacgattt ccggaccgcg caagtccgtg accaacgtga tcctgaaggc gatgctcccg
     2521 gtgctggcaa accgggccga acgcaacatg agccgcaagc ggctgcggac ggcttcgttc
     2581 gccggtgtga tcgcccatcc gagcatgatc agccccaaga tcctctggga actcgggaca
     2641 tatggggccc ttcacgcgcc ggccctcctc cccgcggcat gggcacttgg tggctacgac
     2701 aaccgcccca gcctcagcca catcgagatc ccgacccaga tcgtctgggg ccagcaggac
     2761 cgcctggtcc cggtagcggc ggcctattca tactcgcgac ggatcgagca cgccgaactg
     2821 tcgctggtcg acg
//
LOCUS       DAOTIP010000213         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42074_length_2828_cov_3.152182, whole genome shotgun sequence.
ACCESSION   DAOTIP010000213 DAOTIP010000000
VERSION     DAOTIP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42074_length_2828_cov_3.152182"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="P5138_04960"
     CDS             <1..1556
                     /locus_tag="P5138_04960"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRV59958.1"
                     /translation="GDSRTGLTEGQNQILIDTSEMAPGFHVVRVRVGDNGALGAADDI
                     RRTNIATGQFMVNTPPVADDLSVSTDEGESVTTPLVASDTNADGSHDPLSWSLTTAPS
                     HGFISGEWPNRVYHPEEGFHGNDSFIYSVSDGYGGSDTGTVSIAVNQVDNPVDPPPPP
                     DVGPEKQALKADFEEGRINLNEGAGPATSGIRVVDSTVPDPPVVLDTKHWDKASGEIL
                     APPEDLAFPAKTVSLIITDPLPLELDVTIEFGATGNIGGSFDPDTGAMVLDFDAHALI
                     TVNAPAPLGEVLKCDVTPIPLKLSTSGADLVDPGDGGSRPAATWSASAFDTSGEGAVT
                     SLWNTLPASTPLAEPMAPLATCGDTLDGLIGGKGGFWLGGKVTFDGSANPDPDGGNKG
                     IPKGVALFDGKRLHIRLYCPKIFKPSCKSTSVPVTKKVSRKAGKKAKSKSKPMAKSVR
                     SKIRSGNWKKVSYLIKPKYRAKVRKMVGKPKRLLVIRQTVKAKKMGRKKMKKGKSRTF
                     FHKHKVRAG"
     gene            1588..2520
                     /locus_tag="P5138_04965"
     CDS             1588..2520
                     /locus_tag="P5138_04965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRV59959.1"
                     /translation="MTRGDIGDQGKDATGSAFAGLPVVFGIWLVSVTFVFLPLGLLLT
                     DWIADPAGKSSGGLAGDPVFLAVFVGMVGIWTLIAPLCLTGWADRNIWKKRLGCRDLK
                     PAEHARLDPLWQEVFARAGIHPDAYRLQMSDRPVQNAFAVGDGVITLDQGILSMDDAD
                     LRGIIGHELGHHVRRHTQINMLALWFGAPARILYGILLFLIFGLIRLAGALSGCSWAL
                     IVLFFALALAVPAIALFLLLGIAQLLIRLLDRKSEFEADLYSAEIGFRQEMIGALAEL
                     REIYGDPEPNKPWSPARLNSTHPPFTDRIAALGR"
     gene            complement(2536..>2828)
                     /locus_tag="P5138_04970"
     CDS             complement(2536..>2828)
                     /locus_tag="P5138_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59960.1"
                     /translation="SEDPGPDLTRQEAFIQARDQTMKQVSRKMARRGFEAGRRSGHGH
                     GVIAGGMAAESKVTILVRQQRASSAQSQAAEAQSELAGMTAAPSPPTFTPDQ"
BASE COUNT          496 a          962 c          898 g          472 t
ORIGIN      
        1 taggtgacag ccggacgggg ctgaccgagg gccagaacca gatcctgatc gacacctcgg
       61 aaatggcgcc gggcttccac gtggtccggg tccgggtcgg ggacaacgga gccctgggtg
      121 cagccgacga cattcgccgg accaacatcg cgaccggtca gttcatggtc aataccccgc
      181 cggttgcgga tgacctctcg gtcagcacgg atgaaggcga gtcggtcacg actcccctgg
      241 tcgcttccga caccaacgcc gacggttccc acgacccgct cagttggtcg ctcacgaccg
      301 caccgtccca cggtttcatc tcgggcgagt ggccaaaccg cgtttatcac cccgaggagg
      361 gatttcacgg caatgactca ttcatctact cggtgagtga cggctacggt ggctccgaca
      421 cggggaccgt ctcgatcgcg gtcaaccagg tcgacaatcc ggtcgatccg cctcccccgc
      481 cggacgtcgg cccggagaaa caggcgctga aggcagactt cgaagaaggc cggatcaacc
      541 tgaatgaagg cgcgggaccc gccacctccg gcatcagggt ggtcgactcg accgtgcccg
      601 acccgccggt cgtcttagac acgaagcact gggacaaggc gtccggcgag atcctcgccc
      661 cgcctgagga cctggccttc cccgccaaga cggtcagcct catcatcacc gacccgcttc
      721 cgctcgaact cgacgtgacc atcgagttcg gggcgaccgg caacatcggc ggcagcttcg
      781 acccggacac cggggcgatg gttctcgact tcgatgccca cgccctgatc accgtgaacg
      841 ccccggcccc gctcggcgag gtcctgaaat gtgacgtcac tccgatcccg ctgaagctgt
      901 cgacctccgg agccgacctg gtcgatcccg gcgacggcgg cagccgccct gcggccacgt
      961 ggtcggcgag cgccttcgac acatccggtg aaggagccgt caccagcctc tggaacaccc
     1021 ttcccgcgtc aacgcctctc gccgaaccga tggcgccgct ggccacctgc ggagacaccc
     1081 tcgacggcct gatcggaggc aagggcggct tctggctcgg cggcaaggtc accttcgatg
     1141 gcagcgcgaa cccggacccg gacgggggaa acaagggcat acccaagggc gtggccctct
     1201 ttgacggcaa gcgcctccac atccggctct actgcccgaa gatcttcaag ccttcatgca
     1261 agagcacctc ggtgccggtc acgaagaagg tgagcaggaa ggccgggaag aaagcaaaga
     1321 gtaagtcgaa gccgatggcg aagtcggtca ggtcgaagat caggtccggc aattggaaga
     1381 aggtcagtta cctgatcaag ccgaagtacc gggcgaaggt ccgcaagatg gtcggcaagc
     1441 cgaaacggct gctggtgatc cggcagaccg tcaaggcgaa gaagatgggc cgcaagaaga
     1501 tgaagaaggg caagagccgg accttcttcc acaaacacaa ggtgagagcc ggctgaccgg
     1561 ttcctccccg cgacctatga tcgccgtgtg acgaggggag acatcgggga ccagggcaag
     1621 gacgcgacag gctcggcctt tgccggattg ccggtcgtgt tcgggatctg gctggtctcg
     1681 gtcaccttcg tcttcctgcc gcttggcctg ctgctgacgg actggatcgc cgacccggcc
     1741 ggcaaatcgt ctggcgggct ggccggtgat ccggtctttc tggcggtctt cgtcgggatg
     1801 gtcggcatct ggaccctgat cgccccgctc tgccttaccg gctgggccga ccgcaacatc
     1861 tggaagaaac ggctcggctg ccgcgacctc aaacccgcag aacatgcccg gctagatccg
     1921 ctctggcagg aggtcttcgc acgggcgggc attcacccgg atgcctaccg cctgcagatg
     1981 agcgatcgtc ccgtccagaa cgctttcgcg gtcggtgacg gtgtcatcac cctggatcag
     2041 gggatcctga gcatggacga cgcggacctg cgagggatca tcggccacga actaggccat
     2101 cacgtccgcc ggcacaccca gatcaacatg ctcgccctct ggttcggcgc cccggcccgg
     2161 attctctacg gcatcctgct cttccttatc ttcggtctga tccggctggc gggggccctc
     2221 tcgggctgta gctgggccct catcgttctg ttctttgcgc tcgccctggc cgtaccggcg
     2281 atcgccctgt tcctcctgct cgggatcgcc cagctcctga tccgtctgct cgatcgcaag
     2341 tccgagttcg aggccgacct ctattcggcc gaaatcggct tccgccagga gatgatcggc
     2401 gccctggccg aactccgcga gatctacggt gacccggagc cgaacaagcc ctggtcaccg
     2461 gcccggctga attccaccca tccgcctttc accgaccgga tcgccgccct gggccggtaa
     2521 cccggccgcc gccggctact ggtcgggggt gaaggtcggc ggcgacgggg cagcggtcat
     2581 gccggcgagc tccgactggg cttcggcggc ctgcgactgg gcggaggagg cccgctgctg
     2641 gcgaaccagg atcgtgacct tcgattccgc tgccatgccg ccggcgatca ctccgtggcc
     2701 gtgacccgag cgccgaccgg cttcgaatcc tcgccgggcc atcttgcggg acacctgctt
     2761 catggtctgg tcgcgggcct ggatgaaagc ctcctgcctg gtcaggtcgg ggcccgggtc
     2821 ttccgatc
//
LOCUS       DAOTIP010000214         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42117_length_2826_cov_3.310357, whole genome shotgun sequence.
ACCESSION   DAOTIP010000214 DAOTIP010000000
VERSION     DAOTIP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42117_length_2826_cov_3.310357"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="P5138_04975"
     CDS             complement(<1..1089)
                     /locus_tag="P5138_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV59961.1"
                     /translation="MSVAEIRNPEGTELLGEVELTDPGDVAGVVERAVAARPAWSALP
                     VAERAAMLDRAADLLEPKQEALGRLLSKESGKPIGQAEFEVGGGIHFLRGNAIEGRRL
                     VGRVLPTEGNFGTEWDLAYTRREPLGVVAAILPFNFPVELFVEKCAAALVGGNAVVVK
                     PPVEDPLVVEQFREALIEAGVPSDAIGAVYGDAEVGAALAGAPGVDAISLTGSTAAGI
                     AVATATAARLPFLHLELGGNNACLVLDDADLDLVAGEILRGRVLMNGQACSASKRVIV
                     DRSIHDELAARMSGIVAAQKVGPAPESETTIGPLIHQGAAEKVAAQVANARNQGAEVA
                     AGSGEAEGAYFAPTLLAGQRLDAAKAFWK"
     gene            1274..2047
                     /locus_tag="P5138_04980"
     CDS             1274..2047
                     /locus_tag="P5138_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59962.1"
                     /translation="MRFKSIWTKAVIVLSASIVSLALVGIPASAQAGGVSMGLKARYI
                     FPSGQVKIAYKTKGFKRGSKLTLQRTYGTKQRFRKVLNLKQTKGRFKAVTVAGPATMG
                     RYWYRIVITRNGRFRGKSPARGLLVLDKVPLERMVRYPVSQTVQVGSSLFRYVWFDSS
                     LYRKGDTVLRMDSTSCRALSVQGIGKDTEDADVWFEMVQETGDPSTTFFRTNQLVSTG
                     MGLTGGAFVLKMGSTGYGVDGYVNGFGLCFTKDGRLARR"
     gene            complement(2111..>2826)
                     /locus_tag="P5138_04985"
     CDS             complement(2111..>2826)
                     /locus_tag="P5138_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRV59963.1"
                     /translation="TGGIMVARGDLGIEVPLSKVPVLQKSLIGAAGRASKTVITATQM
                     LASMVSAKRPTRAEVTDVATAIYDGTDAIMLSEETAVGDYPVEAVKVMDQIARGTETD
                     LPYWDWVLNRVRQDEMDVSDSVTQSAVIAAYRLNLQAIVVPTASGRTAKVVAAHRPQV
                     PVLAVTHSVRTQRQLKLIFGVRPVVNDQTTEIRNLLYECADDAVTYGAAASGDLIAIV
                     AGLSDMQLGTNLFEVHRVP"
BASE COUNT          484 a          953 c          896 g          493 t
ORIGIN      
        1 cttccaaaag gccttggccg cgtccaaccg ctgcccggcc agcagggtcg gggcaaagta
       61 ggcgccctcc gcctcgccgg acccggccgc aacctctgcc ccctggtttc tcgcattcgc
      121 gacctgggcg gccaccttct cggccgcgcc ctggtggatc agcgggccga tcgtggtctc
      181 tgattccggc gccggaccaa ctttctgggc cgccacgatc ccggacatcc gcgccgccag
      241 ctcgtcatgg atcgagcgat cgacgatcac ccgcttgctg gccgaacagg cctggccgtt
      301 catcagaacc cggccccgga ggatctcccc cgcgaccagg tcgaggtcgg cgtcgtcgag
      361 taccaggcag gcgttgttgc cgccgagttc gaggtggagg aaggggagcc tggcggcggt
      421 cgccgtcgcc accgcgatgc cggcggcggt tgacccggtg agcgagatcg cgtcaacacc
      481 cggcgccccg gccagggcgg ccccgacctc ggcgtcgcca tagaccgcgc cgatcgcgtc
      541 cgagggcacc ccggcctcga tcagagcctc gcggaactgc tcgaccacga gcgggtcttc
      601 gaccggaggc ttgacgacga cggcattgcc tccgaccagg gcggcagcgc atttctcgac
      661 gaaaagttcg accgggaagt tgaagggcag gatcgccgcg accacgccga gtggctcgcg
      721 gcgcgtgtag gcgaggtccc attcggtgcc gaagttgccc tcggtcggca gcacccttcc
      781 aaccagccgc cggccttcga tcgcgttgcc ccggaggaag tggattccac cgccgacctc
      841 gaactcggcc tggccgatcg gcttgccgga ttccttcgac agcagcctgc caagtgcttc
      901 ctgtttcggt tcgagcaggt cagccgcccg gtcgagcatc gccgcccgct cggccaccgg
      961 aagggccgac caggccggcc tcgcggcgac ggccctttcc accaccccgg caacgtcgcc
     1021 ggggtcggtg agttcgacct cgcccagcag ctcggttcct tccggattcc tgatctcagc
     1081 aacgctcaaa ttcacacctc gaggtacgca tgtacccagc tagacccagt tcgtggtgtt
     1141 cagcctacag aaccgggttc aagatcacga atccttactt cgagagccga tctcgttcaa
     1201 agtaccggcg atccggtccc agtagttttc agttctctga caactgctgg attgcagacc
     1261 gggagggagc gcaatgaggt tcaagtcaat ctggacgaag gccgtaatcg tactttcagc
     1321 gtcgattgtg tcgctggcct tggtagggat tccggccagt gcccaggccg gcggtgtatc
     1381 gatggggctc aaagcccgtt acatattccc ttccgggcaa gtcaagatcg cctacaagac
     1441 gaagggtttc aagcgcggaa gcaaactcac cctccagcgt acttatggca ccaaacagcg
     1501 cttccgcaag gttctgaact tgaagcagac gaagggtcgc ttcaaagccg tcacggtcgc
     1561 cggacccgcg accatggggc ggtactggta ccgcatcgtg atcaccagga acggacgttt
     1621 ccgaggcaag tcgccggccc gtggcctgct cgtgctcgac aaggtgccgc tggagcggat
     1681 ggtgagatac ccggtatcgc agaccgttca ggtcggcagc agcctcttcc gctatgtctg
     1741 gttcgactct tccctttatc gcaagggcga cacggtgctg cgaatggaca gcacctcatg
     1801 ccgtgccctc tctgttcaag gcatcggaaa ggacaccgag gacgcggatg tctggttcga
     1861 gatggtccag gaaaccggcg acccttccac gacatttttc cggaccaacc aactggtcag
     1921 cacggggatg ggcctgaccg ggggtgcctt cgtcctcaag atgggctcga cgggttacgg
     1981 agtcgacggg tacgtgaacg gcttcgggct ctgcttcacg aaggacggtc gtctggccag
     2041 aaggtaggcg ccacgctcgc cgcccaaacc gcgacacaag ttcaccggac cagcgggacc
     2101 ctgaggtgac tcagggaacc cggtggactt cgaagaggtt ggttcccagc tgcatgtccg
     2161 aaaggcccgc gactattgcg atcaggtcgc ccgaggccgc cgccccgtag gtcaccgcgt
     2221 catcggcgca ctcgtagagc aggttgcgga tctccgtggt ctggtcgttt accaccggcc
     2281 gcaccccgaa gatcagcttc agctgccgct gggtcctgac gctgtgggtc acggccagca
     2341 ccggcacctg gggccggtgg gcggccacga ccttcgcggt ccggcccgaa gcggtcggaa
     2401 ccacaatcgc ctgcaggttc agtcggtagg cggcgatcac cgccgattga gtgaccgagt
     2461 cggagacgtc catctcgtcc tgccgcaccc ggttcagcac ccagtcccag tagggaaggt
     2521 ctgtctccgt gccccgggcg atctgatcca tcaccttgac cgcctcgacc gggtagtccc
     2581 cgaccgccgt ctcctcggag agcatgatcg cgtcggtgcc gtcgtagatc gcggtcgcga
     2641 cgtcggtcac ctcggcccgg gtcgggcgct tggccgagac catcgaggcc agcatctggg
     2701 tcgcggtgat caccgtcttg gaagcccggc cggctgcccc gatcaggctc ttctgcagca
     2761 cgggaacctt ggaaagcggc acctcgatgc cgaggtcgcc gcgggcaacc atgatcccgc
     2821 cggtgg
//
LOCUS       DAOTIP010000215         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42545_length_2804_cov_3.384867, whole genome shotgun sequence.
ACCESSION   DAOTIP010000215 DAOTIP010000000
VERSION     DAOTIP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42545_length_2804_cov_3.384867"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..2232
                     /locus_tag="P5138_04990"
     CDS             289..2232
                     /locus_tag="P5138_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59964.1"
                     /translation="MKSKLIAAGAVLVAVLLTGVLGAGAASASSAKPPFYPIDKKTIK
                     VPNDVQPWLPTYSPDGKHIVFQNQLDGSTWATSKDGKGTSCITCEFSDRPDISGGFTY
                     VFPDNKRLFVSHELGGLTGVDSGPNTDAWILECSPSIYDCQSHQYLPVDMSDDKVGVP
                     LIVQRRTWHLAPDGVHLGWMDIRLDGTVMIVGRLTRLDDRYKVVDQKAVNPYRPTSLT
                     DTDAANWAGDNQLWELKSFADGGASILALGGAGYNIDPVKINLKTGKVTRLTANPDWD
                     EDGAISPDGKMLALYSWRTRHRLDAFGWIPQIDSFVEMPAGAALAPFYVSTWPGFQCD
                     LSPWLLSSSGDMGGKLIGQPLNVYPENNLTPGNNLSGNQFWSPNSQDVLLQERTRTIP
                     ATTLSEHVQQKGLTPNRIAIAHIERPAGKKLKVVSSEVGNWAVAPTQYFPSVGSNTSV
                     TVNGKAGGTADLVYTGNLQTGSWSSEYHDYTKDGKTFVNGTYFVSRTIEGVWTLKADV
                     TVTGQHTGRLNMDMTIQGGDPLPIMTGGITAVYDGKKAPALPKLGACYKKLPQKSRLA
                     AQVKSKRAGKATKVTVTVTANVYGDRRPVQNAKVKLAGRIVRTDRSGKAKLKVRGRGT
                     RKVLVTAGDTFIKTSKVAHLKQR"
     gene            2334..>2804
                     /locus_tag="P5138_04995"
     CDS             2334..>2804
                     /locus_tag="P5138_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59965.1"
                     /translation="MPVGYLITLFLLGTCVLLLLAPVRKRPLGLVSFWGGLLVNEQAH
                     WFAVLLAGSTVLAFVEGDISGVARAIAGLAVLAEAGLIVLFLRSFRSRGTLREALAAF
                     DSDSPAALAGRRSYLAALAAPPAMGRSRVRLKSDIPYGPVPGRANRLDQYLPRHG"
BASE COUNT          508 a          945 c          882 g          469 t
ORIGIN      
        1 ctgagccagc ttttccatgt ccagccgggg gccgcggacg aatgcccgca gggcggcgcg
       61 gaaggcgtcg gccggggtcg cgcggggtgc gccgaggccg gcagcttcgt cctggtgggc
      121 ggcgaatgcc ttcgaggttg acgtggtcgc tgcttccatc gaatcgaact ctacagatcg
      181 gtgttacgga aaggtggagt tgcacgaaag tgtacgaata tgatatttgt acacaatcga
      241 atcggaaaca cgacgcgtct acttgcgccg gaaatggaga gagttgggat gaaaagcaag
      301 ctgatcgcgg caggggccgt gctcgtcgca gtacttctga ccggggttct gggggcggga
      361 gcggcgtcgg ccagctccgc caagccgccc ttctacccga tcgacaagaa gacgatcaag
      421 gtgccgaacg acgtccagcc ctggttgccg acctacagcc cggacggcaa gcacatcgtc
      481 ttccagaatc agcttgacgg cagtacctgg gccacctcga aggacggaaa gggcaccagc
      541 tgcatcacct gcgagttctc ggaccggccc gacatcagcg gcggcttcac ttatgtcttc
      601 ccggacaaca agcgtctctt cgtgagccat gaactcggcg ggctcaccgg ggttgacagc
      661 ggccccaata ccgacgcctg gattctcgaa tgctcgccga gcatctacga ctgccagagc
      721 caccagtacc tgcccgtcga catgtcggac gacaaggttg gcgtgccgct gatcgttcag
      781 cgacggacct ggcacctcgc gccggacggc gtccacctcg gctggatgga catccggctc
      841 gacggcaccg tgatgatcgt cggtcgtctg acccggctgg acgatcgcta caaggtggtt
      901 gaccagaagg cggtcaatcc gtaccggccg acctcgctga ccgataccga cgccgccaac
      961 tgggccggtg acaaccagct ctgggagctc aagagcttcg ccgacggcgg cgcctcgatc
     1021 ctggccctgg gcggtgccgg ctacaacatc gacccggtca agatcaacct gaagaccggc
     1081 aaggtcaccc gcctgaccgc caaccccgac tgggacgagg acggcgccat ctcgccggac
     1141 ggcaagatgc tggctctcta ctcatggcgc acccgccatc gcctggacgc cttcggctgg
     1201 atcccgcaga tcgattcgtt tgtcgagatg ccggccgggg ccgcactcgc tcccttctac
     1261 gtctcgacct ggcccggctt ccagtgtgac ctctcgccct ggttgctctc ctcgtccggc
     1321 gacatgggcg gcaagctgat cggccagccg ctgaacgtct acccggaaaa caacctgacc
     1381 ccgggcaaca acctttcggg caaccagttc tggagcccga acagccagga cgtcctgctg
     1441 caggagcgca cccggacaat cccggcgacc acgctctcgg agcatgttca gcagaagggc
     1501 ctgactccga accggatcgc gatcgcccac atcgagcgtc cggccggcaa gaagctgaag
     1561 gtcgtttcct cggaggtcgg caactgggcg gtcgctccca cccagtactt cccctcggtc
     1621 gggtcgaaca cctcggtcac cgtgaacggc aaggccggcg gcaccgccga cctggtctac
     1681 accgggaatc tccagaccgg aagctggtcc tccgagtacc acgactacac caaggacggc
     1741 aagaccttcg tcaacgggac ctacttcgtc agccgcacca tcgaaggggt ctggaccctc
     1801 aaggccgatg tcacggtgac cggccagcac acgggccggc tcaacatgga catgacgatc
     1861 cagggcggcg acccgctgcc gatcatgacc ggcggcatca ctgctgtcta tgacggcaag
     1921 aaggctccgg ccctgcccaa gctgggcgcc tgctacaaga aactcccgca gaagagcagg
     1981 ctcgccgccc aagtcaagtc gaagcgcgcc ggcaaggcga ccaaggtgac cgtaaccgtc
     2041 accgccaacg tctacggcga caggcgaccg gtccagaacg cgaaggtgaa gctcgccggc
     2101 cggatcgtcc gcaccgaccg ctcgggcaag gcgaagctca aggtccgcgg ccgcggcacg
     2161 cggaaagtgt tggtcaccgc cggggacacc ttcatcaaga cctcgaaggt cgcgcacctg
     2221 aagcagcgct gaccaggcaa tcctcgtccc ttccgggtcc tgtcgggcaa cgggccggcc
     2281 gattatggcc ggcccgtttt tcgtgggagg aagccggcaa ctagcttgtg ggaatgcccg
     2341 tcggctacct gatcacgctt tttctgctcg gaacctgcgt gctccttctg ctggctccgg
     2401 tgcggaagcg accactcggc ctggtctcct tctggggcgg gctcctggtc aacgagcagg
     2461 cgcactggtt cgccgtgctg cttgccggtt cgaccgtgct ggccttcgtc gagggcgaca
     2521 tttccggtgt cgcccgggcg atcgcggggc ttgccgtact ggccgaggcc ggactgatcg
     2581 tcctctttct caggtcgttt cggtcccgcg ggaccttgcg ggaagcgctg gccgctttcg
     2641 attcagattc acccgccgca cttgcaggcc ggcgttcata cctggcggcg cttgccgctc
     2701 cgccggcgat gggccgaagt cgggttcggc tgaaatccga cattccctac ggaccggtcc
     2761 cgggccgggc caatcggctc gatcagtacc tgccccggca cggc
//
LOCUS       DAOTIP010000216         2803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42570_length_2803_cov_2.685953, whole genome shotgun sequence.
ACCESSION   DAOTIP010000216 DAOTIP010000000
VERSION     DAOTIP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42570_length_2803_cov_2.685953"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..562)
                     /locus_tag="P5138_05000"
     CDS             complement(80..562)
                     /locus_tag="P5138_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59966.1"
                     /translation="MTSKIKKILASLLALAALALGGAAIASATNGGSDDDHGAQEKAE
                     VNDHDSDANENESTSEGADDNEAEDGQEAADPGEAVSSTVAAKAEAAAVARTGGTVQE
                     VTAETNDAPEKGDDQDPGEKAAPAGTAYEVDLTKGSQELKVSLDRQFNVLQVQTGQDD
                     "
     gene            722..1387
                     /locus_tag="P5138_05005"
     CDS             722..1387
                     /locus_tag="P5138_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV59967.1"
                     /translation="MNLNVLVVEDNPELRRLLNQGLDEEGFEVDLARNGREALMLADP
                     QKHDLLVIDIGLPDSDGRDVCQALKSKGIEAPVLFLTAKDSGPDRVSGFSAGGDDYLT
                     KPFVFAELVARLRVLGRRLTRGDRSTSGDLRLDPVRHVLTCGDRETELTPTEFRLTGA
                     MIARSGEVIRRREMIAAAWPPGAIVHDNTVDVYVRRLRRKLDELESESRIHTVHGVGY
                     RLE"
     gene            1384..2730
                     /locus_tag="P5138_05010"
     CDS             1384..2730
                     /locus_tag="P5138_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRV59968.1"
                     /translation="MKGTIRQRLTFSVVCVTAVMLALLVVGFNLSLRASLDGDVDRLL
                     HARAQATLDNIDLENGKLEVSEGSDDGAEDALVWIYDDRKPLESPQVASQLDRVAASL
                     AAEGSGERDDSDSDTRLFARPVVQNGRVVGTVVTGVSLEPYERTADRAAFASVVLALI
                     MLVLITITTRLVVGRALKPVSEMTEEAAKWSEHDLDRRFNEGEPTDELTRLAATFDTM
                     LDRMAYMVRHERNFSAELSHELRTPLSAISAEAEIALRKDRDAAEYRESLERIAERST
                     ELTKILETLLDVARSEGSGGSDEAADLLDSVDSAVAAAGSLTDRYGVVVELDRPEGRI
                     RAQVAGDTFQRMISPIIENAITYARATVSIAIRSRGRTAEVMIRDDGPGFSPRDLEAA
                     FEPGMRGIAARNEAAPPGTGLGLALARRLARANGGDVTIPVSGDGGTVILTVPIAS"
BASE COUNT          480 a          868 c          938 g          517 t
ORIGIN      
        1 cgtcgaccgg cacgggtaaa cgcggcaaga gaagcccggg ccggagtcga cccgggttcc
       61 tcctccgccc ttgacacgtt cagtcatcct gcccggtctg aacctggagc acgttgaact
      121 gcctgtcgag cgagaccttc aactcctgag agcccttggt caggtctact tcataggcgg
      181 taccggccgg agcggctttc tcgcccgggt cctgatcgtc gcccttctcg ggcgcgtcat
      241 tggtctcggc ggtcacttcc tggaccgttc cgccggtcct ggcaaccgcg gcggcttcag
      301 ccttcgcggc gaccgtgctt gaaacggctt caccggggtc ggctgcttcc tgaccgtctt
      361 cggcttcgtt gtcgtcagcg ccttcagagg tggactcgtt ctcgttggcg tcagagtcgt
      421 gatcgttgac ttcagccttc tcctgcgcgc cgtgatcgtc gtcggagccg ccgttggttg
      481 cagaagcgat cgcggcaccg ccgagggcca gagccgcaag cgccaggagt gacgcgagta
      541 ttttcttgat cttggatgtc atgtgttctt ccttctcatc ggggccgagg ggcatcggcg
      601 cttttccaat gaggcgagta tgtgcgcgga accctgaatt tctcctgacc gggagcggat
      661 tttcaggagt cgttcaggtt tgccaccgat cattggaccg tatgaccgaa gcggcgatca
      721 catgaacctg aacgtcctgg ttgtcgagga caaccccgag ctccggcggc tgctcaacca
      781 ggggctcgac gaggagggat tcgaggtcga tctggcgcgc aacggtcgcg aagccctgat
      841 gcttgccgat ccgcaaaagc acgacctgct ggtgattgac atcgggttgc cggattccga
      901 tggccgcgat gtctgccagg cgctcaagtc gaaggggatc gaggcacccg tgctgttcct
      961 gaccgcaaag gattccggac cggaccgggt ctcgggcttc agcgccggtg gagacgacta
     1021 cctgaccaag ccgttcgtgt tcgccgaact cgtggcccgg ctgcgggtgc tcggcaggcg
     1081 tttgactcgg ggcgatcggt ccacttccgg ggatctcagg ctggatccgg tccggcacgt
     1141 actgacctgc ggggaccgtg agaccgaact gactccgacc gagtttcgcc tgaccggggc
     1201 catgatcgcc cgatcgggag aagtgatccg acgccgcgag atgatcgcgg ccgcctggcc
     1261 accgggggcg atcgtgcacg acaacacggt cgatgtctac gtgcggaggc ttcgcaggaa
     1321 actcgacgaa ctcgagagcg agtcgagaat ccacaccgtt cacggagttg gataccggct
     1381 cgaatgaaag ggacgatccg tcagcgactg actttctcgg tggtctgcgt gaccgcggtg
     1441 atgctggccc tgctcgtggt gggcttcaac ctctcgctgc gtgccagtct cgatggcgac
     1501 gtggaccggc tccttcacgc cagggcccag gcgacgctcg acaacatcga tctggagaac
     1561 gggaaactcg aagtcagcga gggatcggac gacggcgccg aagacgcgct ggtctggatc
     1621 tacgacgacc gaaagccgct tgagtctccg caggttgcca gccagctgga tcgggtggcg
     1681 gcctcactcg ccgcggaggg aagcggggag cgggatgact cggacagcga cacaaggctc
     1741 ttcgcgcggc cggtggtgca gaacggccgg gtagtgggca cggtggttac cggggtctcg
     1801 ctcgagcctt acgagcggac cgcggatcgg gccgccttcg cttcggtggt gctcgccttg
     1861 atcatgctcg tgctgatcac gatcacgacc aggctcgtgg tcggcagggc actcaagccc
     1921 gtctcggaga tgaccgagga agccgcgaaa tggagcgagc acgatctgga ccgaaggttc
     1981 aacgagggcg agcccaccga cgagctgacc aggctggcgg ccactttcga cacgatgctg
     2041 gaccggatgg cctacatggt ccggcacgag aggaactttt cggccgaact ctcgcatgaa
     2101 ctcaggactc ctctttccgc gatatcggcc gaggcggaga tcgcgctcag aaaggatcgg
     2161 gacgcggctg aataccggga atcactggaa cggatcgccg agcgatcgac cgagctcacg
     2221 aagattctcg agaccttgct cgacgtcgcg agatcggaag gcagcggcgg atccgatgaa
     2281 gcggccgatc tccttgactc ggtcgattcc gcggtggccg ctgcgggttc gctgaccgat
     2341 cgatacggcg tcgtggtcga gctggaccgg cccgaaggcc ggatccgggc tcaggtggcc
     2401 ggcgacacgt tccagcggat gatctcgccg atcatcgaga acgcgatcac ctacgccagg
     2461 gccaccgtgt cgatcgcaat ccggtcgagg ggaagaacgg ccgaagtcat gatccgggat
     2521 gacggacccg ggttttcgcc ccgggatctt gaggcggcgt tcgagcccgg gatgcgggga
     2581 attgcggcac gaaacgaagc ggcgccgccc ggtaccggtc tgggcctcgc ccttgcccgt
     2641 cgcctggccc gggccaacgg tggagacgtc acgatccccg tgtcgggcga cggaggaacg
     2701 gtcatcctga ccgttccgat cgcgtcctga cttggcgcct gactaaacgc tgaccgcctc
     2761 cgtctcctcc gtctcctcca tttcggcctc gacgacctcc ggt
//
LOCUS       DAOTIP010000217         2803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42571_length_2803_cov_2.647744, whole genome shotgun sequence.
ACCESSION   DAOTIP010000217 DAOTIP010000000
VERSION     DAOTIP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42571_length_2803_cov_2.647744"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="P5138_05015"
     CDS             complement(<1..390)
                     /locus_tag="P5138_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRV59969.1"
                     /translation="MTDVVALDATAQAELVANGEVSPQELVAGAIQRAEKVNPGINAI
                     CSPLFEQAAEEAAGDLPDGPFRGVPFLFKDLGAGLAGQPMHMGNRALKEIGMTMPYDT
                     HLGSRFRDAGLVTLGRTATPETRTEMRV"
     gene            complement(383..1060)
                     /locus_tag="P5138_05020"
     CDS             complement(383..1060)
                     /locus_tag="P5138_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRV59970.1"
                     /translation="MIRVGVAGAAGRMGATVCGAVEEAEGMELAGRADPALGTDLAEI
                     LPEVDVVVEFTTPDTSLANSIACLEAGVHVVVGATGFDLDKLKDAADNSGTGANCFVA
                     PNFAIGAVLLMELAQKAARHMPECEIIELHHDRKLDAPSGTAKRTEELITAAGGNVHQ
                     PIHSVRLPGLVAHQEVLFGGTGQTLSIRHDSIDRSSFMPGVVLAVRKVGQLEDRFTVG
                     LEKLLDD"
     gene            complement(1057..2334)
                     /locus_tag="P5138_05025"
     CDS             complement(1057..2334)
                     /locus_tag="P5138_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRV59971.1"
                     /translation="MSTDEPRLSETAAGVRVITEEVPSVRSVALGLWVRTGSRDETLA
                     QAGVSHFLEHLLFKGTPNYSAIEISERFDGLGASFNAATGKETTHLHARFLDEHTKDV
                     FDLLGEMLLESTFPDIDSERDVVLEEIAMYEDEPSDRVHDYLADAIFGDTPLGRRILG
                     RSDVISGIPVPDIAAYHQARYSANNIVVAAAGNVDHDEILAMAESLKPGGGSGEETLV
                     PQKPSDTGRLEFAVKDTEQYHVCIGAPGINRADERRFVLAVLDSILGGSSSSRLFTEV
                     REKRGLAYSVGTYSEQFTDTGVVATYVGTREDNVEEACEVIGAELKRIQREPVTAEEL
                     DRAKEHVKGRLVLSGESTAARMARIARAILHDLPLLSLDEMLARVDAVSVEDVSEMAS
                     GLYGEERLSAAAVGRDEGRFRSAIAPVSEALAA"
     gene            complement(2359..>2803)
                     /locus_tag="P5138_05030"
     CDS             complement(2359..>2803)
                     /locus_tag="P5138_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRV59972.1"
                     /translation="IRIYAPTGTQVEDAVARIQSMTKEAEVGDRYQGAKVVKTTTFGA
                     FVELTKGTDGLLHISNVKPGGRVETVEEVLEQGQEIDVTVAEVDRERGRIGLRMTDDP
                     AIAGKSPEEIAALAANSGGGGGGGRRGGDREGGRRRRDRGDRDRR"
BASE COUNT          439 a          937 c          935 g          492 t
ORIGIN      
        1 gacacgcatc tcggttctcg tttccggggt cgcggtccgg cccagggtga ccagaccggc
       61 gtcccggaaa cgagaaccga gatgcgtgtc atacggcatg gtcatcccga tttccttgag
      121 cgcccggtta cccatgtgca tcggctggcc ggccaggccg gcaccgaggt ccttgaagag
      181 gaaggggacg cccctgaacg ggccgtcggg caggtctccg gcggcctcct cggccgcctg
      241 ctcgaagagg ggcgaacaga tcgcgttgat cccgggattg accttctcgg cccgctgaat
      301 cgcgccggca accagttcct gcggggagac ctcgccgttt gcgaccagtt cagcctgggc
      361 agttgcgtcg agagctacga catcagtcat cgaggagttt ctccaatccg accgtgaacc
      421 ggtcttcgag ttgacctacc ttgcgtaccg cgagaacaac accgggcata aacgacgatc
      481 tgtcgatgct gtcgtgacgg atcgaaagcg tctggccggt gccgccgaag agcacttcct
      541 ggtgggcgac cagtcccggc agccggaccg aatggatcgg ctggtggacg ttgccaccgg
      601 ctgccgtgat cagctcctcg gtccgcttcg cggttccgga gggggcgtcc agcttccggt
      661 catggtgaag ttcgatgatc tcgcattcgg gcatgtgccg ggcggccttc tgggccagtt
      721 ccatcagcag cacggcgccg atcgcgaaat tcggcgcgac gaagcagttg gcgccggtcc
      781 cggagttatc ggccgcgtcc ttcagtttgt ccaggtcgaa acccgttgcc ccgaccacca
      841 catgcacccc ggcttcgagg caggcgatcg aattcgccag cgaggtgtcg ggggtcgtga
      901 actcgaccac cacatcgacc tccggcagga tttccgcgag gtcggtgccg agcgccgggt
      961 cggcccggcc ggccagttcc atcccctcgg cctcctcgac ggctccgcat acggttgccc
     1021 ccatgcgacc ggcggcgccg gcgaccccga ctctgatcat gcggcgagag cctcgctgac
     1081 cggggcgatc gccgaacgga agcggccctc gtcccggccc accgcagcgg ccgacaggcg
     1141 ctcctcgccg tagagcccgc tggccatctc cgagacgtcc tccacgctca cagcatcaac
     1201 cctggccagc atctcgtcaa ggctgagcag cggcaggtcg tgaaggatgg cccgggcgat
     1261 gcgggccatg cgggcggcag tcgactcgcc cgagagcacc aggcgaccct tgacgtgttc
     1321 cttggcccgg tcgagctctt cggcggtgac cggctcgcgc tggatgcgct tgagctcggc
     1381 accgatcacc tcgcaggcct cttccacgtt gtcttcacgg gtgccgacgt aggtcgcgac
     1441 cacgccggtg tcggtgaact gttcggagta ggtgccgacc gagtaggcga ggcccctctt
     1501 ctcccgcacc tctgtgaaga ggcgggacga gctggacccg ccgagaatcg agtcgagcac
     1561 ggcaagaacg aaacgccgtt catcggcccg gttgataccg ggcgcaccga tgcagacgtg
     1621 gtactgctcg gtgtccttga ccgcgaactc gagccggccc gtgtcggatg gcttctgcgg
     1681 gacgagggtc tcctccccgc tcccgccgcc cggcttgagc gattcggcca tggcgaggat
     1741 ctcgtcgtgg tcgacattgc ccgcggcagc gaccacgatg ttgttcgccg agtagcgggc
     1801 ctggtggtag gcggctatgt ccgggaccgg aatgccggaa atgacatcgg agcggccgag
     1861 gatccggcgg ccaaggggcg tgtccccgaa gatcgcgtcg gccaggtaat catgaacccg
     1921 gtccgacggc tcgtcttcgt acatcgcgat ttcttccagc acgacatcgc gttcggagtc
     1981 gatgtcgggg aaggtcgatt cgagcagcat ctcgccgagc aggtcgaaga cgtccttcgt
     2041 gtgctcatcg aggaaacggg cgtggagatg ggtggtctcc ttgccggtcg ccgcgttgaa
     2101 ggaggctccg agcccgtcga agcgctcgga aatctcgatc gccgaatagt tcggcgtgcc
     2161 cttgaacaga aggtgttcca gaaagtgaga gacccccgcc tgagcgaggg tctcgtcacg
     2221 tgaacctgtt ctgacccaga ggccgagggc gaccgagcgc accgagggca cttcctcggt
     2281 gatcacccgg acgccggctg cggtctctga aagacgcggt tcgtcagtgc tcatcgggag
     2341 cggggcttcc ggcgccgact atcggcggtc ccggtcgcca cggtcgcgcc ggcggcgtcc
     2401 gccctcgcgg tcaccgccgc gacggccacc gccgcctccg ccgccactgt tggcggccag
     2461 agcggcgatt tcctcggggc tcttgcccgc gatcgccggg tcgtcggtca tgcgcaggcc
     2521 gatccggccg cgctcgcggt cgacctcggc cacggtcacg tcgatctcct gaccctgctc
     2581 gagcacctct tcaacggtct cgacccggcc accgggcttg acgttggaga tgtgcagcag
     2641 gccgtcggtg cccttggtca gctcgacgaa ggcaccgaag gtggtcgtct tcacgacctt
     2701 ggcgccctgg taacgatcgc caacttcggc ttccttggtc atcgactgga tacgggcgac
     2761 cgcatcctcg acctgggtgc cggtcggggc gtagatccgg atc
//
LOCUS       DAOTIP010000218         2800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42627_length_2800_cov_3.350091, whole genome shotgun sequence.
ACCESSION   DAOTIP010000218 DAOTIP010000000
VERSION     DAOTIP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42627_length_2800_cov_3.350091"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="P5138_05035"
     CDS             complement(<1..766)
                     /locus_tag="P5138_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59973.1"
                     /translation="MSRYQHLITDENRKWWTLGAMCLALFMVMLDNTVVNVALPSIQR
                     DLDTSISGLEWIVNGYTLSFAVLLAVGGRIGDIFGRRKAFVFGVIVFTIASTTAGFAP
                     NNFALVASRVLQGIGAAFMMPGTLSIITDAFPPAERGKAIGTWAGVSALALAIGPVLG
                     GFLTENVSWRAIFFINLPVGILAVMAATFVVRESRDTTVGRKVDYTGVAIATVSLTAL
                     VLALIEGNSWGWNSTAIISLIAVSIISLVAFIFTENR"
     gene            complement(763..1332)
                     /locus_tag="P5138_05040"
     CDS             complement(763..1332)
                     /locus_tag="P5138_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRV59974.1"
                     /translation="MTNTGAIETGETNPADAAAAGQELIDNASPEAVKVALQLGTLFR
                     RMLSYGAATTALLEADAHGLSFLEFKTLMGLGVFESQANFCLADISGSTGASMPAVSR
                     AVDSLVRKGLVSRVEDPDDRRRRLVSLTDKGHEVTNAALMGRAVGAVKLATSFTGEEL
                     QEIDSLLSRLIEREDLASIHQQLEGAVHQ"
     gene            complement(1486..>2800)
                     /locus_tag="P5138_05045"
     CDS             complement(1486..>2800)
                     /locus_tag="P5138_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV59975.1"
                     /translation="IGSLTVLPAILAWLGDRVDKGRIPLVTRLRRRKINHGPDSQAGF
                     GARFVRVVLKRPGVSTAVAGGFLVLLAIPAFSLHTVVPGADALPQDLPIVKAYNEVNE
                     AFPGGPAPASVVVQANDVRSPEVQQGVAALKDAVKGQEEFGSPVTTTVSPNGKVEVVN
                     VPLAGAGTDQTSSEALAKLRDEVIPQTVGEVPGAEASVGGMTASSVDFNSLMKNRAPL
                     VFAVVLLTAFILLMVAFRSIVIPVTSILLNLLSVGAAYGLLVWIFQQGHLEGLLGFES
                     IGGITAWLPMFLFVVLFGLSMDYHVFILSRIREAHDKGMSTSEAIEYGLRRTAGPVTS
                     AAIVMVAVFSIFATLSMLEFKQMGVGLAAAVLIDATIVRGVLVPASMKLLGRWNWYLP
                     SWLEWLPKINLEGGPAPTQQAKGNGGSDSPQQRRKSDPLPAGHQV"
BASE COUNT          520 a          888 c          933 g          459 t
ORIGIN      
        1 cccggttctc ggtgaagatg aaagcgacca ggctgatgat cgagaccgcg atcaggctga
       61 tgattgccgt cgagttccag ccccaggagt tgccctcgat cagggcaagg accagggccg
      121 tgaggctgac tgttgcgatg gcaacgccgg tgtagtcgac cttgcgcccg accgtggtgt
      181 cacgcgattc gcggaccacg aaggtcgcag ccatcacggc gaggatcccg accgggagat
      241 tgatgaagaa gatggcccgc caggagacgt tctcggtcag gaagccgccg aggaccggcc
      301 cgattgccag ggcaagagcg gaaactcccg cccaggtccc gatcgccttg ccacgctcgg
      361 ccggcgggaa ggcgtcggtg atgatcgaaa gggtgccggg catcatgaag gcggcaccga
      421 tgccctgcag cacacggctg gcgaccaggg cgaagttgtt cggagcgaat ccggcggtgg
      481 ttgaggcgat cgtgaagacg atcacgccga agacgaaggc cttgcgcctt ccgaagatgt
      541 caccgatccg gccgccgacg gccagcaaca ccgcgaagga gagggtgtag ccgttgacga
      601 tccattcgag accggagatc gaggtgtcga ggtcgcgctg gatcgagggc agggcgacgt
      661 tcacgacggt gttgtcgagc atcaccatga acagcgccag gcacatcgcg cccagcgtcc
      721 accacttgcg gttctcgtcg gtgatcaggt gctggtagcg gctcactggt gtactgctcc
      781 ttccaactgc tggtgaattg aggcgaggtc ttcccgctcg atcaggcgcg agagcagcga
      841 gtcgatctct tgaagttctt cgcctgtgaa gctggtcgcg agcttcacgg cgccgaccgc
      901 ccggcccatc agggcggcat tggtgacctc gtggcccttg tcggtcaggc tgaccagtcg
      961 gcggcggcgg tcgtcagggt cctcgacccg ggagacgagt cccttgcgga ccaggctgtc
     1021 caccgcccgg ctcacggccg gcatcgaagc accggtcgag ccgctgatgt cagccaggca
     1081 gaagttcgcc tgggactcga acacgccgag tcccatcagg gtcttgaatt cgaggaagga
     1141 gagcccgtgg gcatctgcct cgagcagggc cgtcgtcgcg gcgccgtagg agagcatccg
     1201 gcggaagaga gtgccgagct gaagcgcgac cttgacggct tcgggcgagg cgttgtcgat
     1261 caactcctgc ccggccgcgg ccgcatcagc agggttcgtc tcgccggttt cgattgcccc
     1321 cgtattggtc atgacgaaaa tacttgcgct ttcgcaacta tttgtcaacc gcaatagttg
     1381 ccaatgtgat gcacatcaca ggtcgaacga ggcggtgggt cggatcttcc gcgcctctgc
     1441 gggaaatccg acccaccgcc tcgggggaac ggttcgtttg gggattcaga cctggtggcc
     1501 ggcgggcagc gggtcagatt tccgtcgctg ctgcggagaa tccgacccac cgttgccctt
     1561 ggcctgctgg gttggcgccg gaccaccttc gaggttgatc ttcggcagcc attccagcca
     1621 gctgggcagg taccagttcc acctgccgag cagtttcatc gacgcgggca ccaggacccc
     1681 gcggacgatc gtcgcgtcga tcagcaccgc ggcggccagg ccgacgccca tctgcttgaa
     1741 ctcgagcatc gagagggtcg cgaagatcga gaacacggcg accatcacga tcgcggccga
     1801 ggtgaccgga ccggcggtgc gccggagccc gtactcgatc gcctccgagg tgctcatgcc
     1861 cttgtcatgg gcttcgcgga tccggctgag gatgaacacg tggtagtcca tcgacagacc
     1921 gaacaggacc acgaagagga acatcggcag ccaggcggtg atcccgccga tcgactcgaa
     1981 cccgagcagg ccctcgagat gcccctgctg gaagatccag acgagcaacc cgtaggcggc
     2041 acccaccgag agcaggttca gcaggatcga ggtgaccggg atgacgatcg aacggaaggc
     2101 gaccatcagc aggatgaagg ccgtcaacag caccaccgcg aagaccagag gcgcccggtt
     2161 cttcatcagc gagttgaagt cgaccgagct ggcggtcatg cctccgacgc tggcttcggc
     2221 tcccggtacc tccccgaccg tctgcgggat gacttcgtcc ctcagcttcg cgagcgcttc
     2281 gctcgaggtc tggtcggtcc ccgcgccggc gagcggcacg ttgacgacct cgaccttgcc
     2341 gttggggctg accgttgtgg tcacaggcga gccgaactct tcctgtccct tcaccgcatc
     2401 cttcagtgcc gcaacgccct gctggacctc cggtgaacgg acgtcattag cctggacgac
     2461 cacgctggcc ggcgcaggac cgcccgggaa ggcctcgttt acttcgttgt aggccttcac
     2521 gatcggcagg tcctggggca gcgcatcggc gcccggaacc accgtatgaa gcgagaacgc
     2581 cggaatcgcc agcaggacca ggaaacctcc cgccactgcg gtggagacac cgggacgttt
     2641 caggacgacc cgaacgaacc gggccccaaa gccggcctgg ctgtcagggc cgtgattgat
     2701 cttccgccgg cgcaggcggg tcaccagcgg aatgcgtccc ttgtcgaccc ggtcaccgag
     2761 ccaggccagg atggccggga gcacggtgag cgaaccgatc
//
LOCUS       DAOTIP010000219         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42873_length_2787_cov_2.956442, whole genome shotgun sequence.
ACCESSION   DAOTIP010000219 DAOTIP010000000
VERSION     DAOTIP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42873_length_2787_cov_2.956442"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="P5138_05050"
     CDS             <1..206
                     /locus_tag="P5138_05050"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRV59976.1"
                     /translation="AWAYDYKAARRVRKVPVIDPMPMLCPGGWCRAVDGNYLIYRNQG
                     HLTATFTREKYGWIGLKLGDPWR"
     gene            complement(266..592)
                     /locus_tag="P5138_05055"
     CDS             complement(266..592)
                     /locus_tag="P5138_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59977.1"
                     /translation="MKRVFGVAAAFAVLTVGGCGGVANLDPGEVDTVNQAKSAVTNSK
                     VKGALQSGAGHVLNEFMILCREKPLSEVDGDTLREIMVVLAPELKGVNQTYFKKLNRL
                     ATNGCD"
     gene            complement(628..716)
                     /locus_tag="P5138_05060"
     tRNA            complement(628..716)
                     /locus_tag="P5138_05060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Ser,seq:tga)
     gene            complement(799..978)
                     /locus_tag="P5138_05065"
     CDS             complement(799..978)
                     /locus_tag="P5138_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59978.1"
                     /translation="MSKLGDVESEEEIREEMEELGVSNDTTGSPDVLMSGPVIQVTVI
                     ILVVLLVAGALLLVF"
     gene            1074..2255
                     /locus_tag="P5138_05070"
     CDS             1074..2255
                     /locus_tag="P5138_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV59979.1"
                     /translation="MRRLLLLACAVVFLDVTFFSVLTPLLPSYQSDHHLSDGGAGLLA
                     GSFAAGALFFAIPAGWLVASAGARKTVIVGLVAIGICSPLFGFAEQIVVLDLLRFLQG
                     GAGALMWSGAIAWVVVASPTAQRGQMIGIVIAAAVTGELLGAPLGAIAHAVGTEIVFS
                     TVFFAAAVLIVLAMAIPGPADVESQPLAEAWRRIRRSDVPSGLLMLAAPSVAFGLAVV
                     IGPLHMDKLGASPFLIAAAFASGSVIEAVIGPVIGRVSDRVGRTLPYLVGVAAMAVAV
                     IGLGAFGVLPLMFVTVMVMAFGAGLAFTPASTLVTDHATAAGVNQGYASGTSNMAWGG
                     GQMLGAIGGGALAAVSGYLLPCLIAALLLVAVGIVARSLGIGPPVISGQQPEPAPGEQ
                     R"
     gene            2266..>2787
                     /locus_tag="P5138_05075"
     CDS             2266..>2787
                     /locus_tag="P5138_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV59980.1"
                     /translation="MVGENYDARKLASSGRTWKNWSGHQSCRPRDFIRVSGVRELSEV
                     VAAAGSAGRQITTVGSGHSFTPTALSNDLLLDISRTAGVISVDRDSGLVEVGAGTILA
                     DLNRELDHLGLAMPNLGDIDRQTIAGAVSTGTHGTGISLPNISAQIESLKLMGADGEI
                     RELKAGTDELLAAR"
BASE COUNT          431 a          962 c          884 g          510 t
ORIGIN      
        1 aggcctgggc ctatgactac aaggcagccc gccgggtgcg aaaggttccc gtgatcgacc
       61 cgatgccgat gctctgcccc gggggctggt gccgggcggt tgacggcaat tacctgatct
      121 accgcaacca gggccacctc accgccacct tcacccgcga gaagtacggc tggatcggcc
      181 tcaagctcgg cgatccctgg cgctgaggcc tgccactgcg aacgggacgg ccgcgcttgc
      241 ggccagattt tcgaaccgaa tcagttcagt cgcagccgtt cgtggccagc cggttcagct
      301 tcttgaagta ggtctggttg acgcccttca gctcaggcgc gagcacaacc atgatctccc
      361 tcaacgtatc gccgtcgact tccgacaggg gcttctcccg gcaaagaatc atgaattcgt
      421 taagcacatg ccccgcgccc gattggagcg cccctttcac ctttgagttc gtgactgccg
      481 acttggcctg attaaccgtg tcgacctcac cgggatcgag gttcgccact ccaccacagc
      541 cgccaacggt cagcaccgca aacgcggcag caactccaaa gactctcttc aaaggtttct
      601 cctggacggg acgaggttca atatcaacgg agggggaggg attcgaaccc tcgatacgcc
      661 gtaagacgca tgctggtttt caagaccagt gcattcaacc gctctgccac ccctccgtgg
      721 cctggtcggg cccgccgatt ctgacaggcc ggcccgggac ggcgtgccga cacgaacgcc
      781 ggactcctgc tttcagaact agaagacgag cagcagcgcg ccggcgacca gcaggacgac
      841 caggatgatc accgtcacct ggatgaccgg gcctgacatc agcacgtccg gcgagccggt
      901 tgtgtcattg ctgaccccga gctcctccat ctcctcgcgg atctcttctt cggattcgac
      961 atcgcccagt ttgctcatgc cgggaactat cccacgacat cccgtcggag cgctcatccc
     1021 gatcggacga ttcgtccatg ttgactggcg atcgtccctg tcaggatggt gccgtgcgtc
     1081 gtctgctcct tcttgcctgc gcggtcgtct tccttgacgt cacgttcttc tctgtcctga
     1141 cgccgctgct tcccagctac cagtcggacc atcacctctc ggacggtggc gccggcctgc
     1201 tggccgggag cttcgcggcc ggggcgctgt tctttgcgat cccggccgga tggctggtcg
     1261 ccagtgccgg ggcccgcaag accgtgattg tcgggctggt cgcgatcggc atctgcagtc
     1321 ccctgttcgg cttcgccgag cagatcgtcg tcctcgacct gctccgcttc ctgcagggcg
     1381 gcgcgggcgc cctgatgtgg tccggagcga ttgcctgggt cgtcgtcgcc tccccgaccg
     1441 cgcagagggg ccagatgatc gggatcgtga tcgccgccgc ggtgaccggc gagctcctcg
     1501 gcgcgccgct cggtgcgatc gcccacgcag tcggcactga aatcgtattc agcaccgtgt
     1561 tcttcgctgc ggccgtgctg atcgtgctcg ccatggccat ccccgggccc gccgacgtcg
     1621 aatcgcagcc cctggccgag gcctggcgga ggatcaggcg ctcggatgtg cccagcggcc
     1681 tgctgatgct ggccgcgccg tcggtcgcct tcggcctggc cgtggtgatc gggccgctcc
     1741 acatggacaa gctcggtgcc tcgccgttcc tgatcgcggc ggccttcgcc tcgggctcgg
     1801 tgatcgaagc cgtgatcggt ccggtgatcg gccgggtcag cgaccgggtc ggccgcaccc
     1861 tgccctacct ggtcggggtc gccgccatgg ctgtggcggt gatcggcctt ggagctttcg
     1921 gtgtgctgcc gctgatgttc gtaactgtga tggtcatggc cttcggggcg ggattggcgt
     1981 tcacgccggc cagcaccctg gtcaccgacc acgcgaccgc ggcgggggtc aaccagggat
     2041 atgcctccgg cacctccaac atggcctggg gcggagggca gatgctcggc gcgatcggtg
     2101 gcggcgcgct ggcggcggtc tccgggtatc tccttccctg cctcatcgca gccctgctgc
     2161 tggtggcggt cggcatcgtt gccaggtcgc ttggcatcgg gccgccggtg atatccggcc
     2221 aacagcccga acccgcaccg ggcgagcaga gataggctgc cccggatggt cggcgaaaac
     2281 tacgacgccc gcaagctcgc cagctccgga aggacctgga aaaactggtc cgggcaccag
     2341 agttgccgtc cccgcgactt catccgggtc agcggggtcc gggaactttc ggaagtagtc
     2401 gccgcggccg gatccgcggg acggcagatc actaccgtcg gctccggaca ctccttcacc
     2461 ccgaccgcgc tctccaacga ccttctgctc gacatctccc ggaccgccgg ggtgatctcg
     2521 gtcgaccggg attcggggct ggtcgaagtc ggggcgggaa ccatcctcgc cgacctgaac
     2581 cgcgaacttg accaccttgg cctggcgatg ccgaacctcg gtgacatcga ccgccagacg
     2641 atcgccgggg cagtatcgac cggcacccac ggcaccggta tctccctgcc gaacatctcc
     2701 gcccagatcg agagcctgaa gctgatgggc gccgacgggg aaatccggga actgaaggcc
     2761 ggaaccgacg aactgctcgc cgccagg
//
LOCUS       DAOTIP010000220         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42937_length_2783_cov_2.789589, whole genome shotgun sequence.
ACCESSION   DAOTIP010000220 DAOTIP010000000
VERSION     DAOTIP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42937_length_2783_cov_2.789589"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5138_05080"
     CDS             complement(<1..430)
                     /locus_tag="P5138_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA lyase"
                     /protein_id="HRV59981.1"
                     /translation="MAGWQPKVAKADWKRPSRARVRAIRDRLREMYGRPMNLPHRQPI
                     DELVRTILSQATSDANRDRAFAGLKEQFEDWESVRDAPEAEVEAAIRPGGLSRQKAPR
                     IQAVLREIEDLDLGWLEHADRLEAMEFLTGLPGVGRKTAAC"
     gene            complement(434..1369)
                     /locus_tag="P5138_05085"
     CDS             complement(434..1369)
                     /locus_tag="P5138_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRV59982.1"
                     /translation="MDPASQAQVQLGDSRPIACLGEAIVDLICERNLGPGETPEAFVP
                     WPGGALANVAVAIARGGVPSALVGGVGGDGWGRWLAGGLEEAGVSTGWLAFLDGADTP
                     VALVEFGADNEPSFQVYGEHIGPTMAATSGFLDQAVAGSQALIVGSNTMVGETEREVT
                     RRAVAMAAAAGMPILLDPNHRPNRWASDDPAREFCRELTEAATVVKLNRGEAELITGL
                     ADPMAAARELVSLGPELVLVTDGEGTVVTAGAVETSHQPESAPTVSPLGAGDAFMGGL
                     AAGLARLGWDLARAGEVLPGAAAAAAAVCGSWGAQ"
     gene            1439..2221
                     /locus_tag="P5138_05090"
     CDS             1439..2221
                     /locus_tag="P5138_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRV59983.1"
                     /translation="MPALIVRIGDWLPRGWPDLFRQLLLYVLADTVYEVSRGLADGRA
                     NIAFANGERVIDTERTLGLFFEPGLQGLLHNFDWLIDFANTIYLNSQFTVALGFLIWM
                     YLFRNDYYYFFRNMLFVSMGLALVGYIGFPTAPPRMYPEYGFIDTVNAQSSVNHDSAV
                     AKLFINPFAAVPSMHCAVALMIGSTGFMVCKNWFARAFWAGWPLLVAWVTIVTANHYW
                     VDAVLGWGVAGLSFLIASRVLAEIKPETWAWSREAEQQRAEA"
     gene            2236..>2783
                     /locus_tag="P5138_05095"
     CDS             2236..>2783
                     /locus_tag="P5138_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59984.1"
                     /translation="MRLRSLTSPATWGFMAAPLLVVVGLAIVYLVMAPHSADHAAQTF
                     RTELFELSGPTVWNNYWFGGHYLPSYSLLSPPLGAWLGFRVMGSLAVVGTVILFTLIV
                     RREWGERAQAGAIWFAFAATISLFSGRVTFALGALLAMAAVFAAQRDQRIPALILAGS
                     VGLASPVAALFLACLGFSHYLA"
BASE COUNT          394 a         1006 c          887 g          496 t
ORIGIN      
        1 cgcaggcggc cgtcttgcga cctacccccg gcaacccggt caggaattcc atcgcctcga
       61 gacggtcagc gtgctccagc cagccgaggt cgagatcctc gatctcccgg aggaccgcct
      121 ggatccgcgg tgccttctgg cgacttagcc cgccgggacg gattgccgcc tcgacctcgg
      181 cctcaggtgc gtctcgtacg gattcccaat cttcgaactg ttccttgagg ccggcgaatg
      241 cgcggtcgcg gttggcgtcg ctggtcgcct gggagaggat ggtccggacc agctcgtcga
      301 tcggctggcg gtggggcagg ttcatcggcc gtccgtacat ctcgcgaagc cggtcgcgga
      361 tcgcgcggac acgcgcccgg gacggacgtt tccagtcggc cttcgccacc ttcggctgcc
      421 agccagccaa ggcctactgg gctccccagc ttccgcagac cgccgccgcc gcggcagccg
      481 ccccgggcag aacctcgccg gcccgggcca gatcccagcc aagtcgggcc agacccgcgg
      541 ccaggccgcc catgaaggca tcgccggcgc cgagcgggct gaccgtcggc gcggactccg
      601 gctggtggct cgtctcgacc gctcccgcgg taaccaccgt gccttcgccg tcggtgacca
      661 ggaccagttc gggaccgagc gaaaccagtt cccgggccgc cgccatcggg tcggcgagtc
      721 cggtgatcag ctcggcctct ccacgattca gcttgaccac ggtcgctgcc tcggtcagct
      781 cccggcagaa ctcccgggcc gggtcgtcgc tcgcccagcg gttcggccgg tgattcggat
      841 cgagcagtat cggcatcccg gccgcggcgg ccatcgcgac cgcccgccgg gtgacctccc
      901 gttccgtctc cccgaccatc gtgttcgaac cgacgatcag cgcctgggag ccggccaccg
      961 cctggtcaag gaagcccgag gttgcggcca tggtcgggcc gatgtgctcg ccgtagacct
     1021 ggaaggacgg ttcgttgtcg gcaccgaatt cgaccagcgc caccggggtg tcggcaccgt
     1081 cgagaaaggc gagccagccg gtcgaaacac cggcttcctc caggccgccg gccagccacc
     1141 tgccccagcc atcgccaccc acccctccga ccagggccga tggcaccccg ccccgggcga
     1201 tcgccaccgc cacgttcgcg agggctcctc ccggccacgg aacgaaagct tcgggggtct
     1261 cgcccgggcc caggttccgc tcgcagatca ggtccacgat cgcctcgccc agacaggcga
     1321 tcgggcggga gtcgccgagc tgtacttggg cttggcttgc gggatccatc tgcgggaaac
     1381 cgcagcctaa tacagccatc ctgtgagaaa tctgtggacg gcactagact ggtccgtcat
     1441 gcccgcgctg atcgtccgga tcggggactg gctcccccgc ggctggccgg atctcttccg
     1501 tcagctgttg ctctacgtgc tcgccgacac cgtctatgag gtgtcgcgcg gactggcgga
     1561 cggacgcgcc aacatcgcct tcgcgaacgg cgagcgggtg atcgacaccg agcgcaccct
     1621 ggggctcttc ttcgagccgg gcctgcaggg cctgctccac aacttcgact ggctgatcga
     1681 cttcgccaac acgatctacc tgaacagtca gttcacggtc gcgctgggct tcctgatctg
     1741 gatgtacctg ttccgcaacg actactacta cttcttccgc aacatgcttt tcgtctcgat
     1801 gggactggcc ctggtcgggt acatcggctt cccgaccgcg cctccgcgca tgtacccgga
     1861 atacgggttc atcgacacgg tcaacgcgca gtccagcgtc aaccacgatt cggccgtggc
     1921 caagctcttc atcaatccct tcgccgcggt gccgtccatg cactgtgcgg tcgccctgat
     1981 gatcggcagc accggcttca tggtctgcaa gaactggttt gcccgcgcct tctgggcggg
     2041 ctggccgctg ctggtcgcct gggtcacgat cgttactgcc aaccactatt gggtcgacgc
     2101 tgtgctcggc tggggcgtcg ccgggctctc cttcctgatc gcgtccaggg tcctggccga
     2161 aatcaaaccc gagacctggg cctggagtcg cgaggcggag cagcaacggg ccgaggcctg
     2221 acggaggaac cagcggtgcg actccgttcc ctgaccagcc cggcaacctg gggattcatg
     2281 gccgcaccac tgctggtggt ggtcggtctg gcgatcgtct acctggtcat ggcgccccac
     2341 agcgccgacc acgcggccca gaccttccga accgagcttt tcgagctttc gggaccgacc
     2401 gtctggaaca actactggtt cggcgggcac taccttccct cgtactcgct gctgtcaccg
     2461 ccgctcggcg cctggctggg cttccgggtc atgggatcgc tggcggtggt cgggacggtc
     2521 atcctcttca ccctgatagt ccgtcgcgaa tggggcgagc gggcacaggc gggcgcgatc
     2581 tggttcgcct tcgcggcgac gatcagcctg ttctccggca gggtcacctt cgcgcttggc
     2641 gccctgctcg cgatggccgc ggtctttgcc gcgcagcgtg accaacggat tcccgccctg
     2701 atcctggccg ggtcggtcgg gctggccagc ccggttgccg cccttttcct tgcctgcctc
     2761 ggtttctcgc actaccttgc cga
//
LOCUS       DAOTIP010000221         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_42975_length_2781_cov_3.167645, whole genome shotgun sequence.
ACCESSION   DAOTIP010000221 DAOTIP010000000
VERSION     DAOTIP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42975_length_2781_cov_3.167645"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="P5138_05100"
     CDS             <1..188
                     /locus_tag="P5138_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRV59985.1"
                     /translation="WVLTLPAAALIGAATFGVTQLFGNGAVGPVIISAIAIVLIVIAF
                     IRRSRQTEVVDVQAEGV"
     gene            192..437
                     /locus_tag="P5138_05105"
     CDS             192..437
                     /locus_tag="P5138_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59986.1"
                     /translation="MELLTTVVNTKELLDTIAAAFVASLTVAFVSSLGIWGGTKYVDF
                     SQEGRGVSAALALGVGIFGLMATLAIIVLGIYLMVAK"
     gene            447..797
                     /locus_tag="P5138_05110"
     CDS             447..797
                     /locus_tag="P5138_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59987.1"
                     /translation="MAVARNILIIALLAAGVAFLPSGGNVADAVLVTITMAFLAGIAW
                     TVYRLTYEFRTGLLALADSRRIVLYSCFGLVVLLIAGADKMFSTGLGTMAWLLLIASA
                     LVGIWLVVSEARSY"
     gene            801..1658
                     /locus_tag="P5138_05115"
     CDS             801..1658
                     /locus_tag="P5138_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRV59988.1"
                     /translation="MNFRVLSWNLFHGRDFPPLPGGDEPGVPPGGLAWRFLGKPVEGR
                     KHVRLNRDLFHEFAAFLSAAKWDIALLQEVPPRWIPRLADATGSEARPALTSRNWLRP
                     VMSPVARLRPHLAGSWEGGSNLILVRGATPGLERSRPGSEIVDFRRSTLTWLPERRVM
                     SMVELHCGLCVANFHASTGEKAAGDVMRAARLADGRADDRPLVFGGDFNARPGSSDVF
                     ERLEGEFGLAGITSAGSIDHLLTRGVEVLEAAEAWPLVRRDVPDPGTGLMARLSDHAP
                     VVMRISVEK"
     gene            1793..2083
                     /locus_tag="P5138_05120"
     CDS             1793..2083
                     /locus_tag="P5138_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59989.1"
                     /translation="MIGSLPIILLSVLVAAQIAIAGAALWSAGVAARAGARAVLTGKP
                     PAGAAERALPEVLRHGLKVSGRDGVRVGVRVPGLIPGFPETHVYGSSSLGGG"
     gene            2083..2412
                     /locus_tag="P5138_05125"
     CDS             2083..2412
                     /locus_tag="P5138_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59990.1"
                     /translation="MSCRSGQATVEAVAGIAALMLAGLICFQLLATGYTATIADGAAE
                     AGAVALIRGRPVEPAVREALPGWARDRISVSRIGPMVRVRVRPPSLFSSIAESLEVSA
                     EAGGRPG"
     gene            2409..>2781
                     /locus_tag="P5138_05130"
     CDS             2409..>2781
                     /locus_tag="P5138_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59991.1"
                     /translation="MSDQILVGTVGNVRGSDRFALDIASEAAGACIRQDQHDGNRDGI
                     GLLVEVGRERPRPASLLAPAAARELESSLNGSTPGLDARARGNLVCVSAKEADGLPEA
                     VCRLREGNSANPLVVLSTPASY"
BASE COUNT          398 a          884 c          988 g          511 t
ORIGIN      
        1 cgtgggtcct gaccctgcct gcggccgccc tgatcggggc cgccaccttc ggagtcaccc
       61 aactcttcgg caacggggcg gtcggcccgg tgatcatcag cgccatcgcg atcgtcctga
      121 tcgtgatcgc gttcatccgc cgatcccgcc agaccgaagt ggtagacgtc caggcggaag
      181 gggtctgatt catggaactg ctcaccaccg tggtgaacac caaggagttg ctggatacga
      241 tcgccgccgc attcgtggcc tcgctgaccg tggccttcgt ttcctcgctg ggaatctggg
      301 gtggcaccaa gtacgtcgac ttcagtcagg agggcagggg ggtgtcggca gccctcgccc
      361 tcggggtcgg gatcttcggc ttgatggcga ccctggcgat catcgttctc ggcatctatc
      421 tgatggtcgc caagtagggt tgtcccgtgg cggtcgcccg caacattctg atcatcgccc
      481 tgctcgcggc gggggtcgcg ttcctgccca gcggaggcaa tgtcgccgat gcagtgctcg
      541 tcaccatcac gatggctttt ctggccggca tcgcgtggac cgtctatcgc ctgacctacg
      601 agttccggac cggcctgctc gcccttgcgg attcgcggcg gatcgtcctc tactcctgct
      661 tcggactggt ggtcctcctg atcgccggtg cggacaagat gttctcgacg ggcctgggca
      721 ccatggcatg gctgcttctg atcgcttcgg ctctagtcgg catctggctg gtcgtgtcgg
      781 aggcccgcag ctactgatcc gtgaacttcc gggttctcag ctggaatctg tttcacggac
      841 gtgactttcc gcctctgccc ggcggtgatg aaccgggggt gcctccaggg ggcctcgcat
      901 ggcgattcct ggggaaaccg gtcgagggtc gcaagcatgt ccggctgaac cgcgacctct
      961 tccacgaatt cgccgccttc ctctcggccg cgaagtggga catcgccctg ctccaggaag
     1021 tgccgccacg gtggatcccc cggcttgccg atgccaccgg atcggaagca aggccggcgc
     1081 tcacttcccg caactggctg cgtccggtga tgtccccggt cgccaggctg aggccgcatc
     1141 tggccggcag ctgggaaggc ggcagcaacc tgatcctggt ccggggtgcg actcccggcc
     1201 tcgaacgctc ccgtccgggc tcggagatag tcgactttcg ccggtccacc ctgacctggc
     1261 tgccggaacg ccgggtgatg tcgatggtcg aactgcattg cgggctctgt gtggcgaact
     1321 tccacgcgag caccggcgag aaggctgccg gggatgtgat gcgggcggcc cgactggcag
     1381 acggcagggc cgacgacagg ccgctcgtct ttggtggcga cttcaacgcc cggccggggt
     1441 cgagcgacgt gttcgagcgc ctggagggtg agttcggtct ggccgggatc acctcagccg
     1501 gatcgatcga ccatctgctg accagggggg ttgaggtgct cgaagccgcc gaggcgtggc
     1561 cactggtccg gcgggacgtt ccggaccccg ggaccgggct gatggcaaga ctctccgacc
     1621 acgcgccggt cgtcatgcgg atcagcgtcg aaaaatgaac cggcgttgca gatcgttgca
     1681 cctgtagagc cacgtgtgag ggctttcccg tcagcgtcgg ggcatggacg tggaacgggc
     1741 aaggaggtgg gtcgggagac cgggatctga gcgagggtcg gtctcgcttg aattgatcgg
     1801 ctctctgccg atcattctgc tctcggtcct ggtcgccgcc cagatcgcga tagccggcgc
     1861 ggccctctgg tcggccggag tggcagcccg ggccggagcc cgcgcggtgc tgaccgggaa
     1921 accgccggcc ggcgcggcag agcgggcctt gccggaagtt ctgcggcatg gactcaaggt
     1981 ttccgggcgc gacggagtca gggtcggcgt gagggtgccc ggattgatcc ccgggttccc
     2041 cgagacccat gtctacggtt cgagctcgct cggaggcggc tgatgtcctg ccgcagcggg
     2101 caggcgacgg ttgaggcggt tgccgggatc gcagccctga tgctggccgg cctgatctgt
     2161 ttccagttgc tggcgaccgg ttacaccgcg accatcgccg atggcgcggc ggaggccggt
     2221 gcggtggcac ttatccgggg caggccggtc gagcctgcgg tgcgggaagc ccttccgggg
     2281 tgggccagag accgaatctc cgtttcgagg atcgggccga tggtccgggt ccgggtccgg
     2341 ccgccttccc ttttctcctc tatcgcggag agccttgagg tttccgccga ggcgggagga
     2401 cggccaggat gagcgatcag atccttgtcg ggacggtggg caatgtgcgg gggagcgatc
     2461 gattcgctct cgacatcgcg tcggaggcgg ccggcgcctg catcaggcag gaccagcacg
     2521 acgggaatcg ggacgggatc ggcctgctgg tcgaagtcgg ccgggaacgc ccgcgcccgg
     2581 ccagcctgct ggcgccggcc gcggccaggg aactcgaatc gagcctgaac ggttcaaccc
     2641 cgggacttga tgccagggcc cggggcaacc ttgtctgcgt ctcggcgaag gaggcggacg
     2701 gcctgcccga agccgtatgc cggctgcgcg agggcaattc ggcaaatccg ctggtcgtcc
     2761 tgtcaacacc ggccagctac c
//
LOCUS       DAOTIP010000222         2776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43082_length_2776_cov_2.772878, whole genome shotgun sequence.
ACCESSION   DAOTIP010000222 DAOTIP010000000
VERSION     DAOTIP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43082_length_2776_cov_2.772878"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="pyrH"
                     /locus_tag="P5138_05135"
     CDS             <1..361
                     /gene="pyrH"
                     /locus_tag="P5138_05135"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRV59992.1"
                     /translation="GRIVIFAAGIGNPFFTTDTAAALRALEIHAETILMAKNGVEGVY
                     DSDPATNPDAAFIPEITHREAIERGLKVMDSTALSLCMDNDLPIYVFNMADEANMDRI
                     VSGENVGTLVSSRVAGN"
     gene            381..938
                     /gene="frr"
                     /locus_tag="P5138_05140"
     CDS             381..938
                     /gene="frr"
                     /locus_tag="P5138_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRV59993.1"
                     /translation="MIDDLLSDAKDRMAKTVESTRGEFATVRTGRATPHLLDRITVDY
                     YGANTPLNQLANVAASEARLLTVTPYDKGALGLIEKAIQESDIGLTPSNDGNVIRLQI
                     PDLTEERRREMVKVVHGVAEEGRVAVRNVRRDVMADLRELKKEGEVGEDDERRGEDAL
                     QKETDTAIGEIDSMLKGKEEEILEV"
     gene            935..1609
                     /gene="uppS"
                     /locus_tag="P5138_05145"
     CDS             935..1609
                     /gene="uppS"
                     /locus_tag="P5138_05145"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRV59994.1"
                     /translation="MKTPRYVAIITDGNGRWAQGRGLPVKEGHKAGADTVKARLRDAV
                     EFGVRELTVFSFSTENWSRSPEEVSALMEMFSQRIVNETPELHEEGVRMRFIGRREGV
                     SEKLQRQMEEAEAFTGANDTITFFIAFNYGGRAEIVDAARKFSGTTEEEFRQCLYAPE
                     MHDPELLIRTSGEQRISNYLLWQCAYSEFVFRDELWPDFSREAFKASLDEYGNRQRRF
                     GNREPA"
     gene            1609..2403
                     /locus_tag="P5138_05150"
     CDS             1609..2403
                     /locus_tag="P5138_05150"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRV59995.1"
                     /translation="MGETAKRILVAIPWIIVVIAIIIAGGPWFAAALAVFGCLGLREF
                     FQMAEDYRPFQIPAYLSLVGIVASAWLGTQFNVLMFLALPFLLVFFGAARRKNREGVT
                     GSIAVTVLGIYWIGLALAHAVLLRELPDHGAALVVDVLVGTFACDTGAYAVGRMFGSH
                     RFAPNLSPNKTIEGLIGGFLIGMLGVWCAGLYQDWLSGVDALILGAAVAAVAPIGDLF
                     ESMIKRDLDTKDTGNLFGPHGGLLDRLDAVFFTIVVGYYVSVALVY"
     gene            2403..>2776
                     /locus_tag="P5138_05155"
     CDS             2403..>2776
                     /locus_tag="P5138_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRV59996.1"
                     /translation="MARRVLILGSTGSIGTQALEIIAGRDDLEAVGLAVGRDWQTAVA
                     QAKGFSVGTIAVADEASAAEAASSFDGTVLSGEASAREFIGATSPDIVLNGIVGAAGL
                     GPTIAALSEGIEVALANKESLVV"
BASE COUNT          506 a          894 c          892 g          484 t
ORIGIN      
        1 gggccggatc gtgatcttcg cggccggcat cggcaacccg ttcttcacca ccgacacggc
       61 agccgccctt cgcgcgctgg agatccacgc tgagaccatc ctgatggcaa agaacggggt
      121 ggagggggtc tacgacagcg atcccgccac caatccggat gccgcgttca tcccggaaat
      181 cacccatcgc gaagccatcg agcgtggctt gaaggtgatg gattcgaccg ccctcagcct
      241 ctgcatggac aacgacctgc cgatctatgt cttcaacatg gccgatgagg ccaatatgga
      301 taggatcgtt tccggcgaga acgtgggcac gctggtcagc tccagggtcg ccggtaactg
      361 atcgccccta aggaggattg atgatcgacg acctgctgag cgacgccaag gacaggatgg
      421 ccaagaccgt cgagtcaacc cgcggcgagt tcgccaccgt tcgcaccggc cgcgccacgc
      481 ctcacctgct cgaccggatc accgtcgact actacggcgc caacacgccg ctcaaccagc
      541 tcgccaacgt ggccgccagc gaggcccggc tgctcaccgt cactccttat gacaagggtg
      601 ccctcggact gatcgaaaag gccatccagg agtccgacat cggacttact ccttccaacg
      661 acggcaacgt gatccgtctc cagattcccg atctgaccga ggagcggcgc cgggagatgg
      721 tcaaggtcgt tcatggcgtc gccgaggagg gccgggttgc ggtacgcaat gtgcgccggg
      781 acgtcatggc tgacctgcgc gagctgaaga aggaaggtga ggtcggcgag gacgacgagc
      841 ggcgcggcga agatgcgctg cagaaggaga ccgacacagc catcggggag atcgattcca
      901 tgctcaaggg caaggaagaa gagattctcg aggtgtgaaa acaccccgat acgtagccat
      961 catcaccgac ggcaacggtc gctgggccca gggccgcggg ttgccggtca aggaaggaca
     1021 caaggccggc gccgacaccg tcaaggcgcg cctgcgcgac gcggttgaat tcggggtcag
     1081 ggaactgacg gtgttctcgt tctcgaccga gaactggtcc cgttctcccg aggaagtatc
     1141 ggccctgatg gagatgttct cgcagcggat cgtcaacgaa accccggaac tgcatgaaga
     1201 aggtgtgcgt atgcgcttca tcggtcgccg ggaaggggtt tcggaaaagc ttcagcggca
     1261 gatggaagag gccgaggcct tcaccggcgc aaacgacacg atcaccttct tcatcgcctt
     1321 caactacggc ggccgggccg agatcgtcga tgcagcccgg aagttcagcg gcaccaccga
     1381 agaggaattc cggcagtgcc tctatgcgcc ggagatgcac gatccggaac tgctgatccg
     1441 gaccagcggc gagcagcgca tctccaacta cctgctgtgg caatgcgcct actcggagtt
     1501 cgtcttcagg gacgagctgt ggcccgactt ttcgcgagag gcgttcaagg cgtcgctcga
     1561 tgaatacggc aaccgtcagc gccggttcgg caaccgggag ccggcctaat gggtgaaacc
     1621 gcgaagagga ttctggtcgc gatcccctgg atcatcgtgg tgatcgcgat catcatcgcc
     1681 ggagggccct ggttcgccgc ggccctggcg gtctttggct gcctgggcct gcgcgagttc
     1741 ttccagatgg cagaggatta ccggcccttt cagattcccg cctacctgtc actggtcgga
     1801 atcgtcgcct cggcctggct cggcacccag ttcaacgtcc tgatgttcct cgcactgccg
     1861 ttcctgctcg tgttcttcgg tgccgcccgc cggaagaacc gggaaggggt gaccggctcg
     1921 atcgcggtga ccgttctggg catctactgg atcggcctcg ccctggctca cgccgtgctg
     1981 ctcagggaac ttcccgacca cggcgccgcc ctggtcgtcg acgtgctggt cggcaccttc
     2041 gcttgtgaca ccggcgccta cgcggtcggc cgcatgttcg gcagccaccg cttcgccccg
     2101 aacctctcgc cgaacaagac catcgaggga ctgatcggcg gcttcctgat cggcatgctc
     2161 ggagtctggt gcgccggcct ctaccaggac tggctctcgg gagttgacgc gctgatcctg
     2221 ggcgcggcag tagctgccgt ggccccgatc ggggatctct tcgagtcgat gatcaagcgg
     2281 gatctcgaca ccaaggacac cggcaacctc ttcggaccgc acggcggcct gctcgaccgt
     2341 ctcgacgccg tcttcttcac gatcgtggtc ggttactacg tctcagtggc gctggtgtac
     2401 tgatggcgag gagggtcctg atcctgggtt cgaccggttc gatcggcacc caggcgctcg
     2461 agatcatcgc cggccgtgac gatctcgagg cggtcggact cgcagtcggc cgcgactggc
     2521 agaccgccgt cgcccaggcc aagggcttct cggtcgggac catcgcggtg gcggacgaag
     2581 cctccgcggc ggaggccgcc tccagctttg acggaaccgt tctgtcgggc gaagcatcag
     2641 cccgcgaatt catcggggcc acttcgccgg acatcgtcct caacggcatc gtcggggcgg
     2701 ccggactcgg ccctaccatc gccgcgctca gcgaggggat cgaggtcgcc ctcgccaaca
     2761 aggagagcct cgtcgt
//
LOCUS       DAOTIP010000223         2773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43141_length_2773_cov_4.037896, whole genome shotgun sequence.
ACCESSION   DAOTIP010000223 DAOTIP010000000
VERSION     DAOTIP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43141_length_2773_cov_4.037896"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="P5138_05160"
     CDS             complement(<1..892)
                     /locus_tag="P5138_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV59997.1"
                     /translation="MKIFVAGASGAIGLPLVRQLVAAGHQVTGSTRGGGGAGRIREAG
                     GVAVACDVFNRGQLTEAVKQAGPDIVVNQLTSLPPKFEPKKKGFYDANNRIRSEGGDN
                     VIAATAAAGAGRLVTQSISFLYELEGPMIKTEDEAVDRSGTHDAVLGHEQKALEDGRF
                     ETVVLRYGLLYGPGTWYAADGHISEEVRKRRLPVVGDGGGYSSFLHVEDAASAGVVAL
                     DRGEGIYNVTDDEPAPLAEWLPVLAEALEAKPPRRVPAWLASLVAGRAIVRQATDGRG
                     ASNAKFKAETGWQPSIPSWRQ"
     gene            932..1420
                     /locus_tag="P5138_05165"
     CDS             932..1420
                     /locus_tag="P5138_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59998.1"
                     /translation="MPFFICPNCGNRETSGDRTAGFSPRPKGCSKCGFGFVFELLDDY
                     YPAPNAAFFVCDQEGRVIDAGRGSFELTGLTDEDVIGRPVRDVLGLEWIDAGDGGPEA
                     DTNGDGETDPIETSLEWGVRSLGKRVEVRSEKESAAQAIADVFPAYDDDGGLMIVLTP
                     EG"
     gene            1433..>2773
                     /locus_tag="P5138_05170"
     CDS             1433..>2773
                     /locus_tag="P5138_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV59999.1"
                     /translation="MGGRRRNIFVLLFVLGLVIVSAVVCLKKPTQLGLDLQGGIELTL
                     QGRPTPQVKEVTSDAMDRSVDIIRSGCDKLGVSEIEVSRLGADQISVGIPGATNVGTA
                     TECATKPARLYFYNWQDNLIGPARDLSLVGTGADQEQVLKDVRKAWIKAGRPPTEDFN
                     RTWINQGAEPTVWDAVNLASKQPRNDNCENCTSGDKYYLFNEKTHELISGPVTAKEDL
                     YFNDQGRPIPHKGIVKKVPQGTIVVDELPTDADGRTIDDQSQAGYFVVKDDAALSGSE
                     IKDPQQSLDEFNQPNVTFEFTGEGRKAFQDITRTIAQEGQARAIGGVTSSDQAAPLSG
                     NFAIILDGQVVSKPIINFFDNPDGIDGRQGAQISGGFTIDQAQSLAEYLQRGALPIDL
                     KLTSQSQVSATLGQEALDQGVLALLVGLACVIIFLISFYRFLGGVASLALLVYAV"
BASE COUNT          478 a          938 c          876 g          481 t
ORIGIN      
        1 cctggcgcca gctcggtatc gagggctgcc agccggtctc tgccttgaac ttcgcgttcg
       61 aggcgccgcg cccgtccgtg gcctgtcgga cgatagcccg gcccgcaacc agtgacgcga
      121 gccaggccgg gacccgccgc ggcggtttgg cttcgagcgc ttccgcgagc accgggagcc
      181 actcggccag cggagctggc tcgtcatcgg tcacgttgta gatgccttct ccccggtcga
      241 gggccacgac cccagcggag gcggcgtcct ctacgtgaag gaacgaggag tacccgccac
      301 cgtcgcccac aaccggaagg cgccgcttgc ggacttcctc gcttatgtgg ccgtccgccg
      361 cgtaccaggt ccccggcccg tagagcaatc cgtagcgcag cacaaccgtt tcgaagcgac
      421 cgtcttcgag cgctttctgt tcatgcccga gtacggcgtc gtgggtgccg cttctgtcga
      481 ccgcctcatc ttcggtcttg atcatcggac cctcgagttc gtagaggaag gagatgctct
      541 gggtgaccag ccgtccggct ccggccgccg cggtggccgc gatcacgttg tcgccgccct
      601 cggagcggat ccggttgttg gcgtcgtaga accctttctt cttcggctcg aacttcggcg
      661 gcaggctggt cagctggttg acgacgatgt ccggccccgc ctgtttcacg gcctcggtca
      721 actggccgcg gttgaagacg tcacaggcca cggcgacccc gccagcctct ctgatcctgc
      781 cggcgccgcc gccgccccgg gtcgacccgg tcacctgatg gccggcagcg acgagctggc
      841 gaaccagggg gagcccgatg gctccgcttg ctccggcgac gaagatcttc atgaccgcag
      901 cctagggcac cgattcaata cccttcccgg gatgccgttc ttcatctgcc ccaactgtgg
      961 aaaccgggag accagcggtg accggaccgc cgggttcagc ccgcgtccga agggctgctc
     1021 gaagtgcggt ttcggtttcg tgttcgaatt gctggacgac tattacccgg cccccaacgc
     1081 ggcgttcttc gtctgtgacc aggaaggccg ggtcatcgat gcgggccgcg gcagcttcga
     1141 actcaccggc ctcaccgacg aggacgtgat cggccgtccg gttcgcgacg tgctcggcct
     1201 cgaatggatc gatgccggag acggcggccc cgaagccgac accaacggtg acggcgagac
     1261 tgacccgatc gagacctcgc tcgaatgggg cgtccgctcg ctcggaaagc gcgtagaagt
     1321 gcgctccgag aaggaatccg ccgcccaggc gatcgccgat gtcttccccg cctatgacga
     1381 cgacgggggc ctgatgatcg tcttgacgcc agaagggtaa gcttccgctc gaatgggagg
     1441 caggcgccgc aacatattcg tgctgctgtt tgtgctcggt ctggtgatcg tttcggcggt
     1501 cgtctgcctg aagaagccga cgcagttggg actcgacctc cagggaggca tcgagctgac
     1561 cctgcagggc cgtccgaccc cgcaggtcaa ggaagtcaca tccgatgcga tggaccgctc
     1621 ggtggacatc atccgctcgg gctgcgacaa gctcggcgtc tccgagatcg aggtctcccg
     1681 tctcggcgcc gaccagattt cggtcggcat tccgggagcc accaacgtcg gtaccgcaac
     1741 cgaatgtgcg accaaaccgg ctcgcctcta cttttacaac tggcaggaca acctgatcgg
     1801 accggcccgc gacctcagcc tggtcgggac cggagcagat caggaacagg ttctgaagga
     1861 cgtacgcaag gcctggatca aggccggccg tccgccgacc gaggatttca accggacctg
     1921 gatcaaccag ggcgccgagc cgacggtgtg ggacgcggtc aatctcgctt cgaagcagcc
     1981 gcggaacgac aattgcgaga actgcacctc cggtgacaag tactacctgt tcaacgagaa
     2041 gacccatgag ttgatctcgg ggcctgtcac cgccaaggaa gacctctact tcaacgacca
     2101 gggccgtccg atcccgcaca aggggatcgt caagaaggtc ccgcagggca cgatcgtcgt
     2161 cgacgaactg cccaccgacg cggacggccg gacgatcgac gaccagtcgc aggccggcta
     2221 tttcgtggtc aaggacgatg ccgccctctc cgggtcggag atcaaggatc cgcagcagag
     2281 cctggacgag ttcaaccagc ccaacgtgac cttcgagttc acgggcgagg ggcgcaaggc
     2341 gttccaggac atcacccgga ccatcgccca ggaaggacag gcgcgcgcga ttggcggcgt
     2401 cacttcatcc gaccaggcag ccccgctttc cggcaacttc gcgatcatcc tcgacggcca
     2461 ggtcgtctcg aagccgatca tcaacttctt cgacaacccg gacgggatcg acgggcgtca
     2521 gggtgcgcag atctccggcg ggttcacgat cgatcaggcc cagagcctcg cggagtacct
     2581 gcagcgtggc gcccttccga tcgacctcaa actgacctcg cagagccagg tctccgcgac
     2641 cctcggtcag gaagcgctcg atcagggcgt cctggccctg ctggtcggcc tcgcctgcgt
     2701 gatcatcttc ctgatcagct tctaccgctt cctcggtggt gtcgcctcgc tggccctgct
     2761 ggtctacgcg gtt
//
LOCUS       DAOTIP010000224         2773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43155_length_2773_cov_2.983076, whole genome shotgun sequence.
ACCESSION   DAOTIP010000224 DAOTIP010000000
VERSION     DAOTIP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43155_length_2773_cov_2.983076"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="P5138_05175"
     CDS             complement(<1..917)
                     /locus_tag="P5138_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRV60000.1"
                     /translation="MQTVGWWTRPTGFLDRCRRRYGKRFTVNLAAQDPFVVISDPAEI
                     KQVFTAPPDVLHPGEGTRILEPIVGPNSILLLDEGAHMTQRKLVLPAFHGERMRGLES
                     VIAEVTQEQIASWPHGESSPIHPRMQRLTLEVILAAVFGVRSGERLDPLRESLTEMLD
                     FGMNPLSLVPPARRAPFGRGPWARFLKVRARTDELIYGEIQDRRDSGDEGGSEIINSL
                     IAAHHDDGSPMSDVEIHDELLTLLVAGHETTATQLAWTIERLTREPRVLERLVEAVDV
                     GDGEPYLTAVIRESLRLRPVLLNAAPRTV"
     gene            1085..>2773
                     /locus_tag="P5138_05180"
     CDS             1085..>2773
                     /locus_tag="P5138_05180"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HRV60001.1"
                     /translation="MPSLIGRFFGLSLSALFLLSLANAPTASAAYKYPVTVFPSPGTP
                     VASESTTFSFRGVKPRYMGPIKVYGAQSGRHFGRRLAHSDGRGVSFIAYKNFTPGELV
                     RVYTRKKIWGARNGDFAVRIGRFYGNDDKLGHPGAPNKFPALHSRPDLKPPKINILKH
                     TDQAYPGRVFFAPKQTGLTILDSHGRINWFQMTGYGGNGEQVQDFHPQYLNGQQVLTY
                     WKGASTSRGFSQLGYFSVLNRKYNVIARFRVGNGYKPDAHEMTLSPRGTAFTISYRGV
                     LWDTSRFAGGTKTSRVMDNVIQEIDIKTGAVIFEWHSIGNINLEASVNKATNDGAPWD
                     FFHANAISNDGDSILVSARHTCSIYRIDRRTGWLRWRLRGDGRKPKTNDFRLGKGTRF
                     GYQHDIRRLKSGNISMFDNGSAGDSMPAVQKQSSGLILKLTNKGKGRRTATLVHRYKH
                     KPKGVTSGSQGSMTLLPNGNALLGWGSNAQISEITPKGDIAFDATFNTAPTASYRARK
                     AQWMGIPKFRPAIASQGTAKGGTVWASWNGATNIARWRVLSGPDAKHLAVIGDSP"
BASE COUNT          515 a          922 c          858 g          478 t
ORIGIN      
        1 ttgaccgtcc tcggggcggc gttcaggaga acggggcgca gcctgaggct ttcgcggatc
       61 accgcggtca ggtacggctc gccgtcgcct acatcgactg cctcgaccag ccgttcaagc
      121 acccggggtt ccctggtcag ccgctcgatc gtccaggcga gctgggtcgc ggtcgtctcg
      181 tggcctgcca ccagcagggt gagcagctcg tcgtggatct ccacgtccga catcggtgag
      241 ccgtcatcgt gatgggcggc gatcagcgag ttgatgatct cgctgccacc ctcgtccccg
      301 ctgtcgcgcc ggtcctggat ctctccgtag atcaactcgt cggtgcgggc cctgactttc
      361 aggaacctcg cccaggggcc acgaccgaac ggcgccctgc gggccggagg gacgaggctc
      421 agcggattca tcccgaaatc gagcatttcg gtgagcgatt cgcgcagggg gtcgagccgc
      481 tcaccggaac gcaccccgaa cacggcggcc aggatcacct ccagtgtgag gcgctgcatt
      541 cgcgggtgga tcggcgagga ctcgccatgc ggccaggagg cgatctgttc ctgcgtcacc
      601 tcggcgatca ccgattccag cccgcgcatg cgctcgccgt ggaatgcggg caggaccagc
      661 ttgcgctggg tcatgtgggc gccctcgtcc agcagcagga tcgagttcgg cccgacgatc
      721 ggctcgagga tcctggttcc ctcgcccggg tggaggacat cgggcggggc ggtgaagacc
      781 tgcttgatct cggcggggtc ggaaatcacc acgaagggat cctgggcggc caggttgacc
      841 gtgaaccgct tgccgtagcg ccggcggcaa cgatcgagga aaccggttgg tcgcgtccac
      901 cagccaacag tctgcatcgc actgggcatg cgcggtccgg ggggcaaacc ctttgtggct
      961 ctcgtcagca tcatccgtaa cgctagcgcg gatcgccctt gcgattcggt tttgttaccg
     1021 caactatttg cctgacggca gttgcaaccg atagcgtcag tagtccaatt ccaagaaagg
     1081 atcgatgcct tccctgattg gccggttttt cggcctgagt ctctccgccc tgtttctgtt
     1141 gagccttgcc aacgcgccaa ccgcgtcggc cgcctacaag tatccggtca cggtatttcc
     1201 gtctcccgga actccggttg cttctgaaag caccactttc agtttccgcg gggtcaagcc
     1261 ccgctacatg ggaccgatca aggtctatgg cgcccagagt ggccgccact tcggccggcg
     1321 actcgcccat tccgacggtc gcggtgtcag cttcatcgcc tacaagaatt tcactcccgg
     1381 cgaactggtc cgcgtgtaca cccgcaagaa gatatgggga gcccgcaacg gtgacttcgc
     1441 cgtgcggatc ggccgcttct acggcaatga cgacaagctc ggccatcccg gggcaccgaa
     1501 caagttcccc gcgctccatt cgcgcccgga cctgaagccg ccgaagatca acatcctcaa
     1561 gcacaccgac caggcctatc ccggccgggt cttcttcgcg cccaagcaga cggggctcac
     1621 gatcctcgac agccacggcc ggatcaactg gttccagatg accggttacg gaggaaacgg
     1681 cgagcaggtg caggacttcc atccgcagta cctgaacgga cagcaggtcc tcacctactg
     1741 gaagggcgcc tcgacgtccc gcggattcag ccagctcggt tacttctcgg tcctgaaccg
     1801 caagtacaac gtgatcgcga ggttccgggt cggtaacggt tacaagcccg acgcacacga
     1861 aatgaccctt tctccgcggg ggaccgcttt caccatctcc taccgcggcg ttctctggga
     1921 caccagccgg ttcgccgggg gtacgaagac cagccgggtg atggacaacg tcatccagga
     1981 gatcgacatc aagaccggag ccgtgatatt tgaatggcac agcatcggaa acatcaacct
     2041 ggaagcgtcg gtcaacaagg cgaccaacga cggtgcaccc tgggacttct tccacgcgaa
     2101 cgcgatctcg aatgacgggg acagcatcct cgtgtcggcc cgtcacacct gctccatcta
     2161 ccggatcgac cgccgcaccg gctggcttcg ctggcggctc cgcggtgacg gccggaaacc
     2221 gaagacgaat gacttccgcc tcggcaaggg aacccgcttc ggataccagc acgacatccg
     2281 tcgcctgaag agcgggaata tctcgatgtt cgacaacggc tcggccggag attcgatgcc
     2341 ggcggtgcag aagcagtcca gcgggctgat cctgaagctg acgaacaagg gcaagggccg
     2401 ccggacggcg actctggtcc accgctacaa gcacaagcca aagggggtca cctccgggtc
     2461 tcagggcagc atgaccctgc tgccgaacgg aaatgccctg ctcggctggg gttcgaacgc
     2521 gcagatctcc gagatcaccc ccaagggcga tatcgccttt gacgccacgt tcaacacggc
     2581 accgaccgcc tcctaccgtg cccgcaaggc gcagtggatg ggtatcccca agttccggcc
     2641 ggcgatcgcc agccagggga ccgcgaaggg cggcaccgta tgggccagct ggaatggtgc
     2701 gacaaacatc gcccgctggc gggtgctctc cgggcccgac gcgaagcacc tcgccgtgat
     2761 cggcgactcg ccc
//
LOCUS       DAOTIP010000225         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43229_length_2770_cov_2.586372, whole genome shotgun sequence.
ACCESSION   DAOTIP010000225 DAOTIP010000000
VERSION     DAOTIP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43229_length_2770_cov_2.586372"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="P5138_05185"
     CDS             <1..678
                     /locus_tag="P5138_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRV60002.1"
                     /translation="VDFPAVSNYEGDRAGCPNIFTRGLVSPAFLDEELRGLSAWAFDD
                     FLRANDDPALNRLADVDGELIFPDDPGTLPSREGDLAFGMEVYVEMAKAGLTRWDELP
                     ELEAGMRGLEQTRRIDLEEWMDDLGLDAVVFPAVADVGPADADVNPESADRAWANGVW
                     VANGNLVPRHLGIPTVTVPMGLMEDIGMPVGLTFAGRAWDDNALLGLASAFEAIAPRR
                     VPPPRTT"
     gene            771..1814
                     /locus_tag="P5138_05190"
     CDS             771..1814
                     /locus_tag="P5138_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60003.1"
                     /translation="MSLIASLVLGLSAAEASRDSRGSTDSFWATCEYVKAAPAGPAGN
                     FVLVRAGGESIILARSGRDLKVRAYDSYIGDDDLWMTDAGNLDCGIQPTVFNIDRIEI
                     RTPRDWNMGLVLDQRRGAFEPGATGEGKRSAIEIRMTGNPGGRKNASNLYYLGSPGPD
                     VVRAVGDDGRLGLDMNGNGNGGPRDLEFAPTMRVRSLLVKMGRGDDYFSAAGIGIPRN
                     RRPVKAIIYGNAGNDTLVGGPGQDHFDGGYGRDRIRGMGGGDGGGGLDMNVSGGPGHD
                     LIYGGPGNDVIGNSSGDPEPRGGDVIFGGPGRDSILKRNGIRDRINCGPDFELGVQYD
                     AGLDFVKSCAGRY"
     gene            complement(1824..>2770)
                     /locus_tag="P5138_05195"
     CDS             complement(1824..>2770)
                     /locus_tag="P5138_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60004.1"
                     /translation="IIELCWGDVGLLLSMPRQGLGNSAIASVATEEQLEQYGGKWAAM
                     AITEPEAGSDSAAIRTTAVLDGDEYVLNGEKIFVTSGDRADLIVVWASLDRSVGRAAI
                     KSFIVERDNPGLKLDRLEHKLGIRASDTANFTLQDCRVPKNALLGSPEVDVKKGFAGV
                     MKTFDNTRPLVAGMALGVTRAALEETRRRLEVAGVEIDYDSPALSQPAAAARYLEMEA
                     DYEAALMLTLQAAWMADNRKPNSLEASMAKAKAGRTGVDVTLGCVELCGSIGLGEYEL
                     LEKWSRDAKILDIFEGTQQIQLLIVARQLLGKTSAELK"
BASE COUNT          470 a          880 c          939 g          481 t
ORIGIN      
        1 gtcgactttc cggccgtctc caactacgaa ggggaccggg ccggctgccc gaacatcttc
       61 accaggggac tggtcagccc ggcgttcctg gacgaggagc tgcgcgggct ctccgcctgg
      121 gccttcgacg atttcctgcg ggcaaacgac gatcccgccc tgaaccgtct cgccgatgtc
      181 gacggcgagc tgatcttccc cgacgacccg ggcacgttgc ccagccggga gggcgatctc
      241 gccttcggca tggaggtcta tgtggagatg gcgaaagccg gcctgacgcg ctgggacgag
      301 ctgccggaac tggaggcggg catgcgtggc ctggagcaga cccggcgtat cgaccttgag
      361 gaatggatgg acgatctcgg gcttgacgcc gtggtctttc ccgccgtggc cgatgtgggc
      421 ccggccgacg ccgacgtgaa cccggaatcg gcggaccgtg cctgggcgaa cggggtctgg
      481 gtcgcaaacg gaaacctcgt accccggcat ctgggaatac ccacggtcac cgtgccgatg
      541 ggcctgatgg aagacatcgg catgccggtc ggactgacct tcgccggtcg tgcctgggac
      601 gacaacgccc tgctagggct tgcttcggcc ttcgaagcga tcgcgccccg ccgggtaccg
      661 cctccgcgca caacctgagc cggaacttcc gggcgcggga gaggttaagg tggacaatcg
      721 cggtccagaa aagggagatg aatgtgggtc gacctgtcct gatcttcagc atgagcctga
      781 ttgcctcgct cgtacttggc ctatcggcgg ccgaggcgtc tcgtgattcc cgtggcagca
      841 ccgacagctt ctgggcaacc tgtgaatacg tgaaggcggc cccggctggt ccggccggta
      901 atttcgtcct ggtcagggca ggaggagaga gcatcatcct ggctcgttcc ggacgcgatc
      961 tgaaggttcg cgcctacgac tcatacatag gcgacgatga tctctggatg accgatgccg
     1021 gaaacctgga ttgcggcatc cagccgactg ttttcaacat tgaccggata gagatcagaa
     1081 cgccccggga ctggaacatg ggtcttgtgc tcgatcagcg tcgaggggcc ttcgagccgg
     1141 gcgccaccgg cgaagggaag cgttcggcca tcgagatccg catgaccggt aacccgggtg
     1201 ggcgaaagaa cgcttcgaat ctctattacc tggggtcgcc cggacccgac gtggtcaggg
     1261 cggtgggtga tgacggccgg ctgggactgg acatgaacgg caacggaaat ggcggtccgc
     1321 gcgacctcga gttcgccccg acgatgcggg ttcgcagcct gctggtgaag atggggaggg
     1381 gtgacgacta cttctcggcg gccggaatcg gcattccccg caaccggcgc ccggtcaagg
     1441 cgattattta cggcaacgcc ggcaacgaca cgctggtcgg gggacccggg caggatcatt
     1501 tcgacggtgg atacggacgg gaccggatcc gcggcatggg aggcggcgac ggtggcggcg
     1561 gcctcgacat gaacgtttcc gggggccccg gtcacgacct gatctacgga gggcccggaa
     1621 acgacgtgat cggtaacagc agcggcgatc cggaaccccg cggcggagat gtgatcttcg
     1681 gcggcccggg ccgcgactcg atactcaagc gaaacggcat ccgcgaccgg atcaactgtg
     1741 gaccggactt cgaactggga gtgcaatacg acgccggact cgacttcgtg aagagctgcg
     1801 ccggtcgcta ctgaccggcg ccgctatttc agctcggccg aggtcttgcc cagtagctga
     1861 cgggcgacga tcagcagttg gatctgctgg gtgccctcga agatgtcgag gatcttcgcg
     1921 tctcgtgacc acttctccag caactcgtac tcgccgaggc cgatgctgcc gcagagctcg
     1981 acgcagccga gggtcacgtc gaccccggtc cgacccgcct tggccttggc catcgaagcc
     2041 tccagggaat tgggcttcct gttgtcggcc atccacgccg cctgaagggt cagcatcagc
     2101 gccgcttcgt agtccgcttc catctccagg taccgggccg cagccgccgg ctggctcagg
     2161 gccgggctgt cgtagtcgat ctcgaccccg gccacctcga gcctgcgcct ggtttcttcc
     2221 agcgcagcgc gggtgacgcc cagcgccatg ccggcaacca ggggcctggt gttgtcgaag
     2281 gtcttcatca ctcccgcgaa gcccttcttc acgtccactt ccggtgagcc gagcagcgcg
     2341 tttttcggaa cccggcaatc ctggagggtg aagttggcgg tgtccgaggc gcggatgccc
     2401 agcttgtgct cgagccggtc cagcttcagg ccggggttgt cgcgctcgac gatgaaggac
     2461 ttgatcgcgg cgcggccgac cgaacggtcc aggctggccc agacaacgat caggtcggcc
     2521 cggtcaccgg aggtgacgaa gatcttctcg ccgttcagga cgtactcgtc tccgtcgagc
     2581 actgcggtgg tccggatcgc ggctgaatcc gagccggctt ccggctcggt gatcgccatc
     2641 gccgcccact tgccaccgta ttgctcgagc tgctcctcgg tcgcgaccga ggcgatcgcc
     2701 gagttgccga gcccctggcg cggcatcgag agcagcaggc cgacgtcgcc ccagcagagc
     2761 tcgatgatcc
//
LOCUS       DAOTIP010000226         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43260_length_2768_cov_4.421673, whole genome shotgun sequence.
ACCESSION   DAOTIP010000226 DAOTIP010000000
VERSION     DAOTIP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43260_length_2768_cov_4.421673"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="P5138_05200"
     CDS             complement(<1..1024)
                     /locus_tag="P5138_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRV60005.1"
                     /translation="MTENGLNGNGASRGGDDKVRVAIVGVGNCANSFIQGVQYYKDAD
                     PSDQVPGLMHVDLGGYHVNDIEFVAAFDIDQEKVGKDLSEAMWSGQNDTIKFADVPNL
                     GVTVERGMTLDGLGKYLKQVITKAPGPTADIVQILKDTKTDVLVCYLPVGSEDAVKWY
                     VEQAIAAGVGFVNCLPVFIASQDYWDNRFKEAGLPIIGDDIKSQVGATIVHRQLARLF
                     HERGVEMQHTSQLNVGGNMDFFNMLERERLDSKKISKTNAVTSIMGTELEDRDVHVGP
                     SDYVPWLTDRKWAHIRLEGKSFGDVPLNVELKLEVWDSPNSAGVVIDAVRCAKIGMDN
                     GMKGALI"
     gene            complement(1200..1853)
                     /locus_tag="P5138_05205"
     CDS             complement(1200..1853)
                     /locus_tag="P5138_05205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRV60006.1"
                     /translation="MESSSGPGGGQNGPRRPAQEFREQARNALIQSRLTPNAISMMGL
                     VGNLIAAGLVLHGDFFLAGIAFIIGSVMDTLDGRYSRMSGKGTLFGAFLDSTLDRIEE
                     GIVLAAVAWQFADSGDSVAAAMCVVVVLGSLMVSYTRARAEALGVECKVGIATRPVRV
                     VILSAGLVLGGEEVISGFDLLPIAIYVMAGLTAITTLQRVWHVRTELKKLDAAQASE"
     gene            complement(1872..>2768)
                     /locus_tag="P5138_05210"
     CDS             complement(1872..>2768)
                     /locus_tag="P5138_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60007.1"
                     /translation="HRLVRVGMLVYAAALVASYGLSTPMSGNVNRLGALLLGPVLLCA
                     LTAEPLTPKLWRRGVLVLLLPLIVWWQTGPVIRDLELVDDEPAVTKAFYQPLAEALEP
                     RLAREPARVEVVPVASHWESARTAPEFPLARGWERQTDRNLNPLFYADHLGAARYRQW
                     LDRLAVGYVALPNTELDYAGVKEAALIRSGLPYLKRIPVGGDWQLFKVIDARPMVTRP
                     ARLTGLDTDGFTVAAPISGSYEVRIRSTPYWRVESGFGCVEPGPGDWTLVTLDRPGQL
                     KVAADFSPGARFGSDRDCRPDR"
BASE COUNT          487 a          882 c          933 g          466 t
ORIGIN      
        1 cgatgagggc gcccttcatg ccgttgtcca taccgatctt ggcgcagcgc accgcgtcga
       61 tgacgacgcc ggccgagttc ggcgagtccc agacctcgag cttcagctcg acgttcagcg
      121 gcacgtcacc gaaggacttg ccttcgaggc ggatgtgggc ccacttgcga tcggtcagcc
      181 acggcacgta gtccgagggg ccgacgtgga cgtcgcggtc ttcgagctcg gtgcccatga
      241 tcgaggtgac ggcgttggtc ttcgagatct tcttcgagtc gagacgctcg cgctcgagca
      301 tgttgaagaa gtccatgttg ccgccgacat tgagctggga ggtgtgctgc atctcgacgc
      361 cacgctcgtg gaagaggcgg gcgagctggc ggtggacgat ggtcgcgccg acctgggact
      421 tgatgtcgtc accgatgatc gggagtccgg cttccttgaa gcggttgtcc cagtagtcct
      481 ggctggcgat gaagaccggc aggcagttca cgaaaccgac gccggcagcg atcgcctgct
      541 cgacgtacca cttgacggca tcttcggagc cgaccggcag gtagcagacc aggacgtccg
      601 tcttggtgtc cttgaggatc tggacgatgt cggcggtcgg accgggagcc ttggtgatga
      661 cctgcttgag gtacttgccc aggccgtcga gggtcatgcc ccgttcgacc gtgacgccga
      721 ggttcgggac gtcggcgaac ttgatcgtgt cgttctggcc ggaccacatc gcttcggaga
      781 ggtccttgcc gaccttctcc tgatcgatgt cgaaggccgc gacgaactcg atgtcgttga
      841 cgtggtatcc gccgaggtcg acgtgcatga ggccgggaac ctggtcggac ggatcggcgt
      901 ccttgtagta ctggactccc tggatgaagg agttggcgca gttgccgacg ccgacgatgg
      961 ctactcgtac cttgtcgtcg ccgccgcggc tcgcgccgtt tccgttaagt ccgttctcag
     1021 tcatgggtaa gcgaaagtct cctgtggtgg gatggcatct attgccggtt gggctccgga
     1081 ccgcacgctg aaaaggggct gaaaagccac gcctcatccg ggcgcagggc cgggtcgacc
     1141 cctaatgaca ccacaaatcg gcctcggtta caggcatccg gccggaaaag tgacggcctt
     1201 cactcggagg cttgtgccgc gtcgagcttc ttcagctcgg tgcgcacgtg ccagacccgc
     1261 tgcagagtgg tgatcgcggt cagccctgcc atcacatata tggcgatcgg caggaggtcg
     1321 aaaccggaga ttacttcctc cccgccaagc accagcccgg ccgaaaggat cacgacccgg
     1381 accggccggg tggcgatgcc gaccttgcat tcgacaccga gcgcctcggc gcgggcccgg
     1441 gtgtaactga ccatcagcga tccgagtacg acgaccacgc acatggcggc ggccacgctg
     1501 tcgccgctgt cagcgaattg ccaggcgacg gcggccagca cgatgccctc ttcgatccgg
     1561 tcgagagtcg agtcgagaaa ggcgccgaac agggtgccct tgcccgacat cctcgagtag
     1621 cggccgtcca gcgtgtccat caccgaaccg atgatgaaag cgatgccggc aaggaagaaa
     1681 tcaccgtgaa gaaccaggcc ggcggcaatc aggttgccga ccagacccat catcgaaatc
     1741 gcgttcggcg tcagacggga ctggatcagt gcgttgcggg cctgctccct gaattcctgg
     1801 gctggcctgc gcggaccgtt ctggccgcct cccgggccgg acgaagactc catgccggga
     1861 actctagtcc gtcaccggtc gggacggcag tcgcgatcgg agccgaaccg ggcgccgggg
     1921 ctgaaatctg ccgcgacctt caactggccg ggccggtcga gcgtcaccag ggtccagtca
     1981 cccgggcccg gctccacgca gccgaagccg ctttcgacgc gccagtaagg ggtggaacga
     2041 atccgcacct cgtaggaccc ggaaattggc gccgcgaccg tgaagccgtc ggtgtccagc
     2101 ccggtcagcc gggccggccg cgtgaccatc ggcctggcgt cgatcacctt gaagagctgc
     2161 cagtcaccgc cgaccggaat ccgcttcagg tagggcaggc cgctcctgat cagggccgct
     2221 tccttgacgc ccgcatagtc gagttcggtg ttgggcaggg cgacgtagcc gactgcaagt
     2281 cgatcgagcc actgccggta gcgagccgcg ccgaggtggt ccgcgtaaaa gagcgggttc
     2341 aggttgcggt cggtctgtcg ctcccagccg cgggccagcg gaaactccgg ggccgtccgg
     2401 gccgactccc agtgagaggc gaccggaacc acctcgacgc gggcaggctc cctggccaga
     2461 cggggctcaa gggcctccgc cagcggctgg tagaaggcct ttgtgacggc cggctcgtca
     2521 tcgaccagct ccagatcccg gatcaccggg ccggtttgcc accagacgat cagcggcagg
     2581 agcaggacga gaaccccgcg gcgccagagc ttcggcgtaa gcggctcggc tgtcagggcg
     2641 cagagcagaa ccggtccgag cagcagcgct ccgagccggt tgacgttgcc gctcatcggg
     2701 gtacttagac cataggaagc gaccagcgcc gccgcgtaga cgagcatgcc gacccggacc
     2761 agccgatg
//
LOCUS       DAOTIP010000227         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43325_length_2765_cov_3.027675, whole genome shotgun sequence.
ACCESSION   DAOTIP010000227 DAOTIP010000000
VERSION     DAOTIP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43325_length_2765_cov_3.027675"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="P5138_05215"
     CDS             complement(<1..277)
                     /locus_tag="P5138_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60008.1"
                     /translation="MDDDPTRREGEEEMDNGRPDPDDLDPNRMFLRPISEDHVGDLRW
                     IHARPEVVRWWDEPSVDFPWDEPESTRLTILVDGQIGGMIQYWEEDDP"
     gene            367..714
                     /locus_tag="P5138_05220"
     CDS             367..714
                     /locus_tag="P5138_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRV60009.1"
                     /translation="MGQQFLKPLFDRVVIKELEPDRMRNSGLVVPPGTSEPPPQHGIV
                     LAVGQGIDWWEAAGVRMPVRPGDHVVFPAQAGNWVEVDEERLLVCRVTELLGVLENAR
                     DPETPELGGEVRD"
     gene            complement(913..2055)
                     /locus_tag="P5138_05225"
     CDS             complement(913..2055)
                     /locus_tag="P5138_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRV60010.1"
                     /translation="MRSTGKRLLPCLLAAACLLLPGTALADEPAPTISVSPDHFAFGP
                     INVGGELGEPGEFTVTNTGSGTAHLDSAGITGTDHLSFALLANASSCYFLEPAGELAP
                     GASCTLVTIFDPVDEGPHGASLELISDAASSPDVMPLTGTGFPRLLPGATLDPQAADF
                     GSQQSGTASSSRTFTLTSSGSGDLDIGIVTLSGADTDQFRLTSNNCSNRVMASGDSCQ
                     IQVVFLPEGTGSRSASFTVTTDADGDDTTVDLTGTATTTETIRRSRVLTEGRLPRVGR
                     SGKQVVPVRCQTSNMDRCRGRLTLRVRGRALGLRHKRLVTIGSRPYSLSDGNHELGVR
                     LGSRARQSLHRHGNLRVRVISTSRQGDGSARVSDVVRYLRRSRPAA"
     gene            2123..>2765
                     /locus_tag="P5138_05230"
     CDS             2123..>2765
                     /locus_tag="P5138_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRV60011.1"
                     /translation="MALSIGIVGLPNAGKSTLFNALTRNDVLAANYPFATIEPNVGVV
                     GVPDPRLEKLAGIYGSEKILPATVQFVDIAGIVKGASEGEGMGNQFLSNIRESDAICQ
                     VTRVFQDDDITHVEGEVEPKNDIETIRTELILADLQTIENALPRLTKEAKRDKDLEVQ
                     VKAAEAAKEILEGGQGIFEAGFDQEAIRELHLLTAKPLLYVLNCSEDELADDSL"
BASE COUNT          482 a          891 c          917 g          475 t
ORIGIN      
        1 tggggtcgtc ttcttcccag tactggatca tgcccccgat ctgcccgtcg acgaggatgg
       61 tcaggcgggt tgactccggt tcgtcccagg gaaagtccac ggacggctcg tcccaccagc
      121 ggacgacctc gggccgtgcg tgaatccagc gaagatcgcc tacgtggtcc tcgctgatcg
      181 gtctgaggaa cattcggttc gggtcgaggt catcggggtc cggacggcca ttgtccattt
      241 cttcctcccc ctcccggcgg gtcggatcgt catccatgat cggtcaaggc tagccagccg
      301 gctattccga ccccatggca gccgctcttc gcaaccggaa tttccgacac cgcatagtct
      361 tgacccatgg gccagcaatt cctcaaaccg ctgttcgacc gggtcgtgat caaggaactc
      421 gaaccggatc ggatgcgcaa ttccgggctg gtggtcccgc ccggaacctc cgagccgccc
      481 ccgcagcacg gcatcgtgct ggcggtgggt cagggcatcg actggtggga ggcggccggg
      541 gtccggatgc cggtccggcc cggagaccac gtcgtcttcc ccgcacaggc gggcaactgg
      601 gtcgaggttg acgaggaacg gctgctggtc tgccgggtca ccgagttgct cggcgttctc
      661 gagaacgcaa gggatccgga gacccccgaa cttggcggcg aagtaaggga ctgaggctcg
      721 cggggagcgc ctgggtccga tccccccgat cggacccggc gcctccctgc ttgttggttc
      781 cctcccgttt ggaacccgat tgcctgacca aaggtcggac aaccgccctt ataaccggat
      841 aacggcagac gagtttcggt ccggcaaaca aagaagttcg cgaacggttt cccgcttgac
      901 taagccgtga gatcaggctg ccggccgcga ccggcgcagg tatcggacga catcggaaac
      961 cctggcgctt ccgtcgccct gccgcgaggt gctgatcacc cggacacgga ggttgccgtg
     1021 acgatgcagg ctctgcctag cccgggaacc gagcctgact ccaagctcat ggtttccgtc
     1081 gctcaggctg tagggccggc tgccgatcgt cacgagccgt ttgtgccgca ggccgagcgc
     1141 ccggccgcgg actctgagag tcaaccggcc acggcaacgg tccatgttcg acgtctggca
     1201 gcggaccggc accacctgct tgccggaccg gccaacccgt ggcagcctgc cttcggtcag
     1261 gacccggctc cgcctgatcg tttccgtcgt cgtagcggtg ccggtcaggt cgaccgtggt
     1321 gtcgtcaccg tcggcgtcgg tcgtcaccgt gaaactggcg gaccggctgc cggtcccttc
     1381 cggcaggaag acgacctgga tctggcagct gtcccccgat gccatgaccc ggttcgagca
     1441 gttgttggag gtcagcctga actggtcggt gtcggccccg gaaagagtca cgatcccgat
     1501 gtcgagatcc ccgctcccgc tgctggtcag cgtgaatgtc cgggatgagg aagcggtgcc
     1561 ggactgctgg cttccgaagt ccgctgcctg cggatccagc gtggcccccg gcaagagccg
     1621 gggaaagccg gttccggtca gcggcatcac gtcgggagag cttgcagcgt cggagataag
     1681 ctcgagactc gccccatgcg gtccctcatc gaccggatcg aagatcgtga ccagggtgca
     1741 tgaggcgccg ggggcaagtt cgccggcggg ctcgaggaaa tagcagctgc tcgcgttggc
     1801 cagaagcgcg aaggagagat gatctgtgcc ggtgatgccg gccgagtcca ggtgggcggt
     1861 tccggagccg gtgttcgtga ccgtgaactc gccgggctca ccgagttcgc ctcccacgtt
     1921 gatcgggccg aaggcgaagt ggtcagggct gaccgagatc gtcggtgccg gctcatccgc
     1981 cagtgcggtt cccggcagaa gcaggcaggc cgcggccagc aggcacggga gcagtcgttt
     2041 cccggtcgat ctcatggtca tatcttcgaa cacgcgcatt gcgaagacgt tgccgtgccc
     2101 gggtccgtag tcttcccggg cgatggccct ctcgatcgga atcgtcgggc ttcccaacgc
     2161 ggggaagtcg accctcttca atgccctgac caggaacgac gtgctcgccg cgaattatcc
     2221 gttcgcgacg atcgagccca acgtgggcgt ggtcggcgta cccgatcccc ggctcgaaaa
     2281 gctggccggg atctacggct cggaaaagat ccttcccgcc acggtccagt tcgtggacat
     2341 cgccggcatc gtcaagggcg cctcggaggg agaggggatg ggcaaccagt tcctgtcgaa
     2401 catccgcgag tccgacgcga tctgccaggt gacccgggtc ttccaggacg acgacatcac
     2461 ccacgtcgag ggcgaagtcg aaccgaagaa cgacatcgag acgatccgca ccgagctgat
     2521 cctcgccgac cttcagacga tcgagaacgc cctgccccgg ctgaccaagg aagcaaagcg
     2581 ggacaaggac ctcgaggtgc aggtgaaggc ggccgaagcg gcgaaggaga ttctcgaggg
     2641 cggccagggc atctttgaag ccgggttcga tcaggaagcg atccgcgagc tgcacctgct
     2701 gaccgccaag cccctgctct acgtcctcaa ctgctcggag gatgagctgg ccgacgactc
     2761 gctga
//
LOCUS       DAOTIP010000228         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43421_length_2759_cov_4.343195, whole genome shotgun sequence.
ACCESSION   DAOTIP010000228 DAOTIP010000000
VERSION     DAOTIP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43421_length_2759_cov_4.343195"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="P5138_05235"
     CDS             complement(<1..1742)
                     /locus_tag="P5138_05235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRV60012.1"
                     /translation="MPPSVLTSADFETEAFGLIAELEGEADSGVRNSGLDDRLEQLRE
                     VWRKLDPDDQAESRTLTGRIAALVKNRPGERPGEDPPRPLTPAEAAGQTVLIADEQEE
                     AALALQGLEGIELTEPVQTRIFEGEANPDRLLAHMGYDEFRPGQRDAVSAALEGRDSL
                     IVMPTGGGKSLCYQLPGLASSSLTLVVSPLIALMADQIKRLRSEGHPAVMFASGLPEE
                     EAIASWQSVQDGSARIAYCSPERFGSAAFLDLIGSREIDLLAIDEAHCLSEWGHDFRP
                     DYLRLPRVAERLGRPPVMACTATATPQVVAEIAERMGMTDPVEIHSGFDRPNLSFDTI
                     PLEGKGSKARKQALLRIGLTDPENRPAIVYCGTRKSTEEVAEDLRSEGISAVAYHAGM
                     APDERASAQHRFMEGDAEVVVATNAFGMGIDKANVRSVWHWAIPSSVEAYYQEAGRAG
                     RDGKPARAVLLAMRADLGRLVRFNEMRRIDPSDVLSYVDDIRRAAGHDVDGNPGSVTI
                     EAPRSDGERTRLAVGERSGALLVEPAPGGQLLVTPGPETGHGPVRAACRVAEDRGWVA
                     YRAVAAFSFSTTCRR"
     gene            complement(1905..2264)
                     /locus_tag="P5138_05240"
     CDS             complement(1905..2264)
                     /locus_tag="P5138_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60013.1"
                     /translation="MTMTARPIEISVHNALVLATAPLLMVVPYLLTFSPGVGFLTLFL
                     GAALMGVSLAGASPKRPLSLAAQSGFDWAIGIAIFSIGILAGIAGQDPMTTIFLVGFG
                     AAHLALTVSTRYSARSA"
     gene            2364..>2759
                     /locus_tag="P5138_05245"
     CDS             2364..>2759
                     /locus_tag="P5138_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRV60014.1"
                     /translation="MTAVSSNHAAAGTVQPVMARRSRLDILLAERGLFSSRTAAARAI
                     RAGQVKVGRDGPIALRPSQEVSLDQEIEILESQKYVSRGGTKLENGLDQLGLEVEGRL
                     CLDVGASTGGFTDCLLQRGAKHVIALDVAY"
BASE COUNT          463 a          887 c          952 g          457 t
ORIGIN      
        1 cggcggcagg tggtcgaaaa gctgaatgcc gcgaccgccc ggtaggcgac ccagccgcgg
       61 tcctcggcga cccggcaggc ggcgcgcacc ggaccgtggc cggtctcggg tcccggcgtg
      121 acgagcagct gccccccggg agccggttcg accagcagcg ctccggaacg ctcgccgact
      181 gcgagccggg tccgttcacc gtccgaacgg ggggcctcga tcgtgaccga gcccggattg
      241 ccgtcaacgt cgtggccggc ggcccggcgg atgtcatcga catacgaaag gacgtcggat
      301 gggtcgatcc ggcgcatttc gttgaagcgg acgagccttc ccaggtctgc gcgcatcgcc
      361 agcagcaccg cccgtgcggg cttgccgtcc cgtccggccc ttcccgcttc ctggtagtag
      421 gcctcgacac tcgagggaat cgcccagtgc cagaccgacc gcacattcgc cttgtctatt
      481 cccattccga aggcgttggt tgcgacgacc acctcggcgt caccttccat gaaccggtgc
      541 tgggcggagg cgcgctcatc cggcgccatc ccggcgtggt aggcgaccgc ggaaatgcct
      601 tcggaccgga gatcctccgc cacctcctcg gtgctcttcc gtgtgccgca gtagacgatc
      661 gccggacggt tctccgggtc ggtgaggccg atccggagca gtgcctgctt gcgagccttg
      721 gatcccttgc cctcgagcgg gatcgtgtcg aagctgaggt tcgggcgatc gaacccggaa
      781 tggatctcga ccggatcggt cattcccatt cgttcggcga tctccgccac cacctgggga
      841 gtggcggtgg ccgtacaggc cattaccggc ggccggccga ggcgctcggc gacccggggg
      901 agccgcaggt agtcgggccg gaagtcgtgt ccccactcgg aaaggcagtg ggcctcgtcg
      961 atcgccagca gatcgatctc ccgcgagccg atcaggtcga gaaaggcagc ggaaccgaac
     1021 cgttccgggg agcagtaggc gatccgggcg gacccgtcct ggaccgactg ccaggaggca
     1081 atcgcctcct cctccggaag ccccgaggcg aacatcacgg ccggatggcc ctcggagcga
     1141 agtcgcttga tctggtcggc catcagggcg atcagcgggc tcacgaccaa agtcagggat
     1201 gaagaggcca gccccggcag ctggtagcag aggctcttgc cgccgccggt cggcatcacg
     1261 atcaggctgt cgcggccctc gagcgccgcc gaaactgcgt ctcgctggcc ggggcgaaac
     1321 tcgtcgtacc ccatgtgggc cagcagacgg tctggattgg cctcgccctc gaagatccgg
     1381 gtctggaccg gttcggtcag ttcgatacct tcgagcccct gcagggcaag tgcggcctcc
     1441 tcctgctcgt ccgcgatcag gacggtctgg ccggccgcct cggccggagt aagggggcgg
     1501 ggcgggtcct cgccaggccg ctctccggga cggtttttga ccagggcggc gatccggccg
     1561 gtcagggtcc gcgattcggc ctgatcgtcc gggtcgagct ttcgccagac ctcccggagc
     1621 tgctccagac ggtcatcgag cccgctgttc cggaccccgg aatccgcctc gccttcgagt
     1681 tcggcgatca ggccgaaagc ctcggtctcg aaatcggcgg atgtgagaac ggatgggggc
     1741 accggtccag actaaagaca gcgtctgacg ccgaaaattc accccccgaa cccgtgcaag
     1801 gaaagagccc cgctgggagg gcggggctct tggtgttgct tagggaggag agagattcaa
     1861 tgatctctta tgtgtttttg atcgatggga ggtaggtggg ctctttatgc gctgcgggcg
     1921 ctataccgcg ttgaaacggt aagtgccaag tgagcggcgc cgaacccgac caggaagatt
     1981 gtcgtcatcg gatcctgtcc cgcgatgccg gccaggatgc caatggaaaa gatcgcaatg
     2041 ccgatggccc agtcgaaacc ggactgcgcg gccagcgaaa gcggacgctt ggggctggct
     2101 ccggcgaggg agacgcccat cagggccgca ccgaggaaca aggtcaggaa accgactccc
     2161 gggctgaagg tcaggaggta agggacgacc atgagcagcg gcgcggtggc cagcacgagt
     2221 gcgttgtgaa cgctgatttc tatgggtctc gcggtcatgg tcatgtttct cctgctcaca
     2281 aggtaggggt ccacttactt tttgtgtgtg agtcgaatca cagttgacat ggccgcttgt
     2341 ccgtccgcga gaggcggagc cggatgacgg ccgtatcttc gaaccacgcc gcggccggta
     2401 ccgttcagcc ggtcatggcc aggcgatccc gactcgacat cctcctcgcc gagcggggac
     2461 tcttcagctc ccggaccgcg gcggcccggg cgatcagggc cggccaggtg aaggtcggac
     2521 gcgacgggcc gatcgccctt cgccccagcc aggaagtgag cctcgaccag gagatcgaga
     2581 tcctcgaatc gcagaagtat gtgtcgaggg gcggcacgaa gcttgagaac gggctcgacc
     2641 agctcggcct ggaggtggaa gggaggctct gcctcgacgt cggcgcctcg accgggggct
     2701 tcaccgactg tctgcttcag cggggcgcga agcatgtgat cgccctcgac gtggcctac
//
LOCUS       DAOTIP010000229         2755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43517_length_2755_cov_3.192963, whole genome shotgun sequence.
ACCESSION   DAOTIP010000229 DAOTIP010000000
VERSION     DAOTIP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43517_length_2755_cov_3.192963"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="P5138_05250"
     CDS             complement(<1..453)
                     /locus_tag="P5138_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV60015.1"
                     /translation="MEWRSAGDYSDILYEKAEGIAKITINRPEVRNAFRPQCLAELRD
                     AFEKARDDTEVGAIIFTGAGKEAFCSGGDQRIRGDDGYIGDDSVAKQGVGRLDVGDLH
                     VQIRRTPKPVIAMVAGYAIGGGHILHLVCDMTIAGDNARFGQTGPRVGS"
     gene            586..>1890
                     /gene="menE"
                     /locus_tag="P5138_05255"
     CDS             586..>1890
                     /gene="menE"
                     /locus_tag="P5138_05255"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HRV60016.1"
                     /translation="MKLDNWLAQRAQSCPDRTALIADGESMTYAELEEEAVHAARRLA
                     AWGVRRGATVAMNMGPGRDKVVLVHALMKTGAVLLPISPKLSPEERARAIESCGVSVD
                     LDDPGKLTQTEADMPLLGEHDMADPHCRILTSGSSGTPHTVTLSYGNHLFSAMGSAFN
                     IGVDPSDRWLCALPMSHISGLSIVMRSVIYGTAMVLQDRFEPAAVADAIERDGANVIS
                     LVSTMLLRLLEEGVDLTAPRAILVGGGPVPQSALSEALDRGARVIQTYGLTETCSQVT
                     TLEVAEARRKVGSVGRPLLTSHLRIQGGEILVQGPTVSAGALDADGWLHTGDVGRIDE
                     DGFLYVQGRTDEMIITGGENVIPAEVEDVLVQHPEIVEAAVVGRPDPEWQQAVTAVVV
                     ARDGVEVELDEVRSHCEGKLAPYKIPKAVEVVTELPRTPSGKL"
     assembly_gap    1891..1900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1934..>2755
                     /gene="ilvC"
                     /locus_tag="P5138_05260"
     CDS             1934..>2755
                     /gene="ilvC"
                     /locus_tag="P5138_05260"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRV60017.1"
                     /translation="MTTQDIFYDKDGDLSKLEGKTVAILGFGSQGHAHALNLKDSGVN
                     VVVGLREGSASREQAAAAGLEVLSVADAASKGDIVMILLPDEKQAEIWENEISDGIAE
                     GNLLMFAHGFAIRFDQIVPPAGVDVGMVAPKGPGHMVRRQFEEGSGVPCLMAVEQDAT
                     GNAHDLVLAYAIGIGGGRAGIIETTFKDECETDLFGEQAVLCGGATELVRAGFDTLVE
                     AGYDPRLAYFECLHELKLIVDLMYEKGIQGMRYSISNTAEYGDLTRGKRVIGEESR"
BASE COUNT          483 a          897 c          904 g          461 t
ORIGIN      
        1 gctgccgacc cgggggccgg tctggccgaa gcgggcattg tccccggcga tcgtcatgtc
       61 gcagaccaga tgaaggatgt gaccgccgcc gattgcgtag ccggcaacca tggcgatcac
      121 cggcttcggg gtgcgtctga tctgcacgtg gaggtccccg acgtccagtc ggcccacccc
      181 ctgcttcgcg acgctgtcat caccgatgta tccgtcgtca ccccggatcc gctggtcgcc
      241 acccgagcag aaagcctctt tcccggcgcc ggtgaagatg atggccccga cctcggtgtc
      301 gtcccgggcc ttttcgaagg catcacgcag ttccgccaga cactgggggc ggaaggcatt
      361 gcgcacttcc gggcggttga tcgtgatctt cgcgatgccc tcggccttct cgtagaggat
      421 gtcggagtaa tctccggccg accgccactc gatcggctcg taaagcgtct cttccggggg
      481 cgcgtttcgg tgtctttcgg gcaagtccgg tactccgttc gtcaggctgc cgccgtggag
      541 gcggcggatc gcaagaattg agggacttac gataaacgag gtcaattgaa actcgacaac
      601 tggctggccc agagagcgca gagctgcccc gaccgcaccg ccctgatcgc ggacggcgag
      661 tcgatgacct acgcggaact cgaggaggaa gccgtccacg ccgcacgccg cctcgctgcc
      721 tggggagtgc gccggggagc cacggtcgcg atgaacatgg gaccgggccg cgacaaggtg
      781 gtcctcgttc acgccctgat gaagaccggt gcggtgctgc tgccgatcag cccgaagctg
      841 tcaccggagg aaagggccag ggcgatcgag tcctgcggcg tctcggtcga cctcgacgat
      901 cccggcaagc tcacccagac cgaggcggat atgcccctgc tcggcgagca cgacatggcc
      961 gacccgcact gccggatcct caccagcggc tccagcggca ccccgcatac ggtgacgctg
     1021 tcgtatggca accatctctt cagcgcgatg gggtcggcct tcaacatcgg ggtggacccc
     1081 tccgatcgat ggctctgtgc gctcccgatg agccatatct ccggtctctc gatcgtaatg
     1141 cgctcggtga tctacggaac ggcgatggtg ctccaggatc gtttcgagcc agccgcggtc
     1201 gccgacgcga tcgagcggga cggagccaac gtgatctcgc tggtttccac catgctgctg
     1261 cggctgctcg aggaaggggt cgacctcacc gctccccggg caatcctggt cggtggagga
     1321 ccggtgccgc agagcgcgct gtccgaggct ctcgaccggg gcgcccgggt gatccagacc
     1381 tacgggctga ccgaaacctg ctcccaggtg acgaccctcg aagtggccga agcgcgtcgc
     1441 aaggtcggat cggtggggag gcccctgctg acctcgcatc tgcgtatcca gggcggcgag
     1501 atcctcgtcc agggtccgac cgtttcggcc ggcgcactcg atgccgacgg ctggctgcac
     1561 accggtgacg tcggccggat cgacgaggac ggttttctct acgtccaggg acgcacggac
     1621 gaaatgatca tcaccggcgg cgagaacgtg atcccggccg aggtcgagga cgtgctggtc
     1681 cagcacccgg agatcgtcga agcggcggtg gtcggccgcc ccgatccgga atggcagcag
     1741 gccgtcacgg ccgtcgtcgt ggcgcgagac ggagtcgagg tcgaactcga tgaagtgcga
     1801 agccattgcg agggcaaact cgccccgtac aagatcccca aggcggtcga ggtggtaacc
     1861 gagctgccca ggaccccgtc gggcaagctc nnnnnnnnnn caccgtgcgg ccctgctcaa
     1921 atagcatcac cgtatgacca cccaagacat cttctacgac aaggacggcg atctctcgaa
     1981 gctcgagggc aagaccgtcg ccatcctcgg tttcggttcc cagggccacg cacatgccct
     2041 gaatctcaag gactcgggtg tcaatgtggt ggtcggcctg cgtgagggct cggccagtcg
     2101 tgaacaggct gccgccgcgg gcctcgaggt tctgtccgtc gccgatgcag ccagcaaggg
     2161 cgacatcgtg atgatccttc tgccggacga aaagcaggcg gagatctggg agaacgagat
     2221 cagtgacggg atcgccgaag gcaacctgct catgttcgcc cacggtttcg ccattcgctt
     2281 cgaccagatc gtgcccccgg ccggcgtcga cgtcggcatg gtcgccccca agggccccgg
     2341 ccacatggtc cgtcgccagt tcgaggaagg ctcgggcgtg ccctgcctga tggccgtcga
     2401 gcaggacgcg accggcaacg ctcacgacct cgtcctcgcc tacgcgatcg ggatcggtgg
     2461 cggccgcgcc gggatcatcg aaacgacctt caaggacgaa tgtgaaaccg acctcttcgg
     2521 tgagcaggcg gtcctctgtg gcggtgccac cgaactggtc cgggccggat tcgacaccct
     2581 ggtcgaagcg ggttacgacc cccgtctcgc ttacttcgag tgcctccatg aactcaagct
     2641 gatcgtcgac ctcatgtacg agaagggcat tcagggcatg cggtactcga tctcgaacac
     2701 cgccgagtac ggcgacctga cccggggcaa gcgggtgatc ggtgaagaat caagg
//
LOCUS       DAOTIP010000230         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43600_length_2751_cov_3.715875, whole genome shotgun sequence.
ACCESSION   DAOTIP010000230 DAOTIP010000000
VERSION     DAOTIP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43600_length_2751_cov_3.715875"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2751
                     /locus_tag="P5138_05265"
     CDS             <1..>2751
                     /locus_tag="P5138_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRV60018.1"
                     /translation="PEDIKRLGRLQPGKLFLVDLEKNRIVEDEEVKREISTQQPYGEW
                     FENASVHFDDLPTAYVTMTGIQPTPRRQKAFGYTQEDLRVLITPMAATGAEPIGSMGN
                     DVALPVLSDNNPPLYSYFKQLFAQVTNPPIDPIREEIVMSLAAGVGAELNLLSESEQH
                     AHQLRLDQPILLNQELETIRHVNHDIFEADTIDITWDSSDGAAGLETRLAAICMEAER
                     AIERGFNILILSDRRTGPRRVPIPALLAVGAVHHHLVREGTRLKAGMILESGEPREVH
                     HMATLIGYGCSAINPYLMLDTVDDLVVQGLVPDMESPEKAQRNVVKAIGKGLLKTISK
                     MGISTTQSYCGAQIFEAVGLESDLIDRYFTGTASRIGGIGLDVLAQETMDRHASAFPG
                     GSDDLLPVGGVYAWRRDGEFHQWNPETVSLIQHAVKGEGGSAQEKYDEFSQQVNEIAL
                     KRATLRGLFKLRTADGGPIPIEEVEPAKEIVKRFATGAMSLGSISRESHENLAIAMNR
                     LGGKSNTGEGGEDPVRFTPDDNGDLRRSAIKQVASGRFGVTFHYLANADQLQIKMAQG
                     AKPGEGGQLPGHKVDRYIGSVRHTTPGVSLISPPPHHDIYSIEDLKQLIYDLRCSNPK
                     ASVSVKLVAEVGVGTVAAGVAKANADHVLIAGHDGGTGASPLSSVQSAGIPWEIGLAE
                     TQQTLIRNDLRSRITVQTDGQLKTGRDVVVAAILGADEMGFSTAPLIASGCIMMRACH
                     LNTCPVGIATQDPELRKRFKGQPEHVVNYFFFVAEEARKIMAGIGVRSVDELIGRIEL
                     LETDDAVDHWKAKGIDLSGLLAWPEGIDPSVDRHRTRPQEPVLDDNLDWKLIENARSA
                     IDGDGPVEFDMDVKNVDRCVGGIMSNAIANTQGAKGLPEDTIKVNFRGAAGQSFGGWL
                     APG"
BASE COUNT          516 a          950 c          864 g          421 t
ORIGIN      
        1 gccggaagac atcaagcgcc tcggacgcct gcagcccggc aagcttttcc tggtcgacct
       61 tgagaagaac cggatcgtcg aggacgagga agtcaagcgc gagatctcga cccagcagcc
      121 ctacggcgag tggttcgaga acgcctcggt ccacttcgac gacctgccca ccgcctacgt
      181 gacgatgacc ggcatccagc cgaccccccg ccggcagaag gccttcggat acacccagga
      241 agacctgcgg gtcctgatca ccccgatggc ggcgaccgga gccgagccga tcggctcgat
      301 gggcaacgac gtcgctctgc cggtgctctc ggacaacaac ccgccgcttt acagctactt
      361 caaacagctc tttgcccagg tcaccaatcc gccgatcgac ccgatccggg aagagatcgt
      421 gatgagcctc gccgccgggg tcggcgccga gctcaacctg ctcagcgaat cggagcaaca
      481 cgcccatcag ctgcggctcg accagccgat cctgctcaac caggagctcg agacgatccg
      541 ccacgtcaat cacgacatct tcgaggcgga cacgatcgac atcacctggg actcctccga
      601 cggagccgcc ggccttgaaa cgcgcctcgc cgcgatctgc atggaggccg agcgggcgat
      661 cgagcgcgga ttcaacatcc tgatcctctc cgaccggcga accggcccgc gccgggttcc
      721 gatccctgcc ctgctggcgg tcggcgcggt ccatcaccac ctggtcaggg aaggcacccg
      781 cctcaaggcc gggatgatcc tcgagtcggg cgagccccgc gaggttcacc acatggccac
      841 cctgatcggc tacggatgct cggcgatcaa cccttacctg atgctcgaca cggtcgacga
      901 cctggtcgtg cagggcctcg tcccggacat ggagtcgccc gagaaggccc agcgaaacgt
      961 ggtcaaggcg atcggcaagg gcctcctgaa gacgatttcg aagatgggta tttcaaccac
     1021 ccagtcctac tgtggcgccc agatcttcga agcggtcggc ctcgagagcg acctgatcga
     1081 ccgctacttc accggaactg cctcccggat cggcggcatc gggctcgatg ttctcgccca
     1141 ggagacgatg gaccgccacg cgtccgcatt ccccggtggc agcgacgacc tgctcccggt
     1201 cggcggcgtg tacgcctggc gccgcgacgg cgagttccac cagtggaacc cggagaccgt
     1261 ttccctgatc cagcacgcgg tcaagggtga gggcggatcc gcccaggaga agtacgacga
     1321 gttttcgcag caggtcaacg agatcgcgct caagcgggcc acgctccgcg gtctgttcaa
     1381 actgaggacc gccgacggcg gcccgatccc gatcgaagag gtcgagccgg cgaaggagat
     1441 cgtcaaacgc ttcgccaccg gcgcgatgtc gctcggctcc atctcacgcg agagtcacga
     1501 aaaccttgcg atcgcgatga accgcctcgg cggcaagtcg aacaccggcg agggcgggga
     1561 ggacccggtc cgcttcacgc ccgatgacaa cggggacctg cgccgctcgg cgatcaagca
     1621 ggtcgcttcc ggccgcttcg gcgtgacctt ccactacctg gccaatgccg accagctcca
     1681 gatcaagatg gcgcagggcg cgaagcccgg agagggcggg cagctgcccg gacacaaggt
     1741 cgaccgctac atcggctcgg ttcgccacac cacgcccggg gtgagcctga tctcgccgcc
     1801 gccgcatcac gacatctact cgatcgagga cctcaagcag ctgatctatg accttcgctg
     1861 ctcgaacccg aaggcctcgg tctccgtgaa gctggtcgcc gaagtcgggg tcggcacggt
     1921 cgccgccggt gtcgccaagg caaacgccga ccacgttctc atcgccggcc atgacggggg
     1981 caccggcgct tcgccgcttt cctcggtcca gtcggcaggc atcccctggg agatcggcct
     2041 cgccgagacc cagcagacgc tgatccgcaa cgacctgcgt tcgcgcatca cggtccagac
     2101 cgacggccag ctgaagaccg gccgggatgt cgttgtcgcc gccatcctcg gggctgacga
     2161 gatgggcttc tcgactgccc cgctgatcgc ttccggctgc atcatgatgc gggcctgcca
     2221 cctgaacacc tgcccggtcg gaatcgccac ccaggatccc gagctcagga aacgcttcaa
     2281 gggccagccg gagcatgtcg tgaactactt cttcttcgtc gccgaagagg cccggaagat
     2341 catggccggg atcggcgtgc gcagcgtcga tgagctgatc gggcggatcg aactgctcga
     2401 gaccgatgac gcggtggatc actggaaggc gaagggaatc gacctttcgg gtctgctcgc
     2461 ctggccggag ggcatcgacc cgtcggtcga tcgccaccgg acccgccccc aggagccggt
     2521 gctcgatgac aatctcgact ggaagctgat tgagaacgcc cggtcggcga ttgacggtga
     2581 cggcccggtc gagttcgaca tggacgtcaa gaatgtggat cgctgcgtcg gcggcatcat
     2641 gtcgaacgcg atcgccaaca cccagggagc gaaggggctc cccgaggaca cgatcaaggt
     2701 caatttccgc ggcgcagccg gtcagtcttt cggcggctgg ctcgcccccg g
//
LOCUS       DAOTIP010000231         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43808_length_2741_cov_2.897990, whole genome shotgun sequence.
ACCESSION   DAOTIP010000231 DAOTIP010000000
VERSION     DAOTIP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43808_length_2741_cov_2.897990"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2000
                     /gene="infB"
                     /locus_tag="P5138_05270"
     CDS             <1..2000
                     /gene="infB"
                     /locus_tag="P5138_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRV60019.1"
                     /translation="NAPAGNPPAPAASSGSSAPASGGSGAAGKKRRRVVIDSQASRRE
                     QGGAPPPQRPPRRRGGRRRRPLLEEPEPVQADPAVPRATKVNSGVTVRDLAELLDLSS
                     ADLIKKLMTMGEMATLTQTLTDESVKAIADEYDRKIEIVSAAEEEVEAPEFEDSADQL
                     VDRPPVVTIMGHIDHGKTSLLDAIRETEVAEGEAGGITQHIGAYQVHHDTHTITFLDT
                     PGHEAFTAMRARGAQVTDVAVIVVAADDGVMPQTREAIDHARAADVPMLIVVNKIDKE
                     GAQPDRIRNDLAAEGLTPEAWGGDTVYCDVSAKTHQGLDNLLDMILLVTELEELSANP
                     NAPASGSVIESQLDPGRGPVVTVLVQRGTLRVGDSVVAGPVWGRVRAMHDYHGEKVDE
                     AVPGMPVEVLGFDGVCDAGEFVQAVENDRRARQLAQERLNRLKAESIARRQARNVTLD
                     EIFARAQTGDLKELNIVLKSDVAGSLEALQDEIAKVPQEQVAINIIRAQTGGITESDV
                     MLASASNAIIIGFNVRPLADARRAAQSEGVDVRTYSVIYKITEDLRAAMEGMLDTVEV
                     EDSLGEAEVREVFKASKVGRIAGCVVTEGRIQRDASVRLVREGTVIWTGQLDSLRRFK
                     DAVQTVEEGQDCGIVLDGYADVKVGDVLEFFSTRQVEQTLS"
     gene            2027..2374
                     /gene="rbfA"
                     /locus_tag="P5138_05275"
     CDS             2027..2374
                     /gene="rbfA"
                     /locus_tag="P5138_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRV60020.1"
                     /translation="MRRVNEVLRQVISDAITNDLSDPRLEMATVTSVDTTPDLRHAKV
                     YFTVLGDDQAKADALNGLKRAHSLLQGKVAAETRMKHTPSLVFEYDDSIESGMRIDAL
                     LAEGESAGPEDGE"
     gene            2371..>2741
                     /locus_tag="P5138_05280"
     CDS             2371..>2741
                     /locus_tag="P5138_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRV60021.1"
                     /translation="MNPAAASQLVVKGSLEEIAALIREEQKFLLTTHEGPDGDALGSL
                     LGTHYLLRSLGKDSVMFLAAKEFPLPIEYRHLPLEDVFHEPPADVRDRIVVFLDCGNI
                     DRMPVDFLREDGKLMVNIDHH"
BASE COUNT          517 a          918 c          881 g          425 t
ORIGIN      
        1 ggaacgcgcc cgccgggaat cccccggcgc cggccgcatc ttccgggtca tccgcccccg
       61 ccagtggcgg gtcgggcgcg gccggcaaga aacgccgccg tgtcgtgatc gattcccagg
      121 cctcgcgtcg cgagcagggt ggtgcgccac ccccgcagcg accgccccgc cgtcgtggtg
      181 gccgccgccg tcgtccgctg ctcgaagagc cggagcccgt tcaggccgat ccggccgttc
      241 cccgggcgac caaggtcaat tccggcgtga cggtcaggga cctcgccgaa ctgctcgacc
      301 tgagctcggc cgacctgatc aagaagctga tgaccatggg cgagatggcg acgctcaccc
      361 agaccctcac cgacgaatcg gtgaaggcga tcgccgacga gtacgaccgc aagatcgaga
      421 tcgtctccgc ggcagaagag gaagtcgaag cgcccgaatt cgaggattcc gccgatcagc
      481 tcgtcgaccg cccgccggtt gtcacgatca tgggtcacat cgaccacggc aagacttcgc
      541 tgctcgacgc gattcgcgag accgaggtgg ccgaaggtga agccggcggc atcacccagc
      601 acatcggcgc ctaccaggtc caccacgaca ctcacacgat caccttcctc gacaccccgg
      661 gccatgaggc gttcaccgcc atgcgtgccc gcggggccca ggtcaccgat gtggcggtga
      721 tcgtggtcgc tgccgatgac ggcgtcatgc cgcagacccg cgaggcgata gaccatgccc
      781 gcgccgctga cgtgccgatg ctgatcgttg tcaacaagat cgacaaggaa ggcgcacagc
      841 ccgaccggat ccgcaacgac ctcgcggccg aaggactcac tcctgaagca tggggcggcg
      901 acaccgtcta ttgcgacgtt tccgcgaaga cccaccaggg gctcgacaac ctgctcgaca
      961 tgatcctgct ggtcaccgaa ctcgaggagc tctcggcgaa cccgaatgcg ccggcctccg
     1021 gttcggtgat cgagtcccag ctcgatccgg gccgcggtcc cgtggtcacc gtgctggtcc
     1081 agcgcggaac cctgcgggtc ggagactcgg tcgtggccgg gcccgtctgg ggccgggtca
     1141 gggcgatgca cgactaccac ggtgaaaagg tcgacgaggc agtgccaggc atgccggtcg
     1201 aggtactcgg attcgacggc gtctgtgacg ccggcgagtt cgtccaggcg gtcgagaacg
     1261 accgccgggc ccgccagctc gcgcaggaga ggctcaaccg cctcaaggcc gagtcaatcg
     1321 cccgccgtca ggcccgcaac gtcaccctcg acgagatctt cgcccgggcc cagaccggtg
     1381 acctcaagga actcaacatc gtcctcaaga gcgatgtcgc cggctccctg gaagcgcttc
     1441 aggacgagat cgccaaggtg ccgcaggaac aggtcgcgat caacatcatc cgcgcccaga
     1501 ccggcggcat caccgaatcc gacgtcatgc tggcctcggc ctcgaacgcg atcatcatcg
     1561 gcttcaacgt ccggcccctg gccgatgccc gccgggccgc ccagtccgag ggcgtggacg
     1621 tccgcactta ttccgtcatc tacaagatca ccgaagacct ccgggccgcc atggagggca
     1681 tgctcgacac cgtcgaagtg gaggattcgc ttggcgaggc cgaagtcagg gaagtcttca
     1741 aggcttccaa ggtcggccgg atcgccggat gtgtcgtcac cgaagggaga atccagcgcg
     1801 atgcttcggt gcggctcgtc agggagggga ccgtcatctg gacggggcag ctcgattcgc
     1861 tgcgccggtt caaggacgcg gtccagaccg tcgaggaagg acaggactgc ggcatcgtcc
     1921 tggacgggta cgccgacgtg aaggtcggtg acgtactcga gttcttctcg acccgtcagg
     1981 tcgaacagac cctcagctag gcggtcagaa ggtgtcgaag ggtcggatgc gcagggtgaa
     2041 tgaggtcctt cgtcaggtga tctccgatgc gatcaccaac gatctgagcg atcctcgtct
     2101 ggagatggca acagtcactt cggtcgatac cacccccgat ctgcgtcacg ccaaggtgta
     2161 cttcaccgtg ctcggtgacg atcaggcaaa ggccgacgca ctgaacggcc tgaagcgggc
     2221 ccacagcctg cttcagggaa aggtggcggc ggagacccgg atgaaacaca cgccctctct
     2281 ggtttttgaa tacgacgact cgatcgaatc cggcatgagg atcgatgccc tgctggccga
     2341 aggcgaatca gcgggtccgg aggacggcga gtgaacccgg ctgcagccag ccagctcgtg
     2401 gtgaagggca gcctcgagga gattgccgcg ctgatccgcg aggagcagaa gttcctgctc
     2461 accacccatg aagggcctga cggtgatgcg ctcggctccc tgctcggcac gcactatctg
     2521 ctcaggagcc tcggcaagga ctccgtgatg ttcctggccg ccaaggagtt cccgctgccg
     2581 atcgaatacc gccacctgcc cctcgaggac gtctttcacg agcccccggc cgacgtacgg
     2641 gaccggatcg tcgtcttcct cgattgcggc aacatcgatc ggatgccggt cgacttcctc
     2701 cgcgaggacg gcaagctgat ggtgaacatc gatcaccatc a
//
LOCUS       DAOTIP010000232         2734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_43978_length_2734_cov_3.558044, whole genome shotgun sequence.
ACCESSION   DAOTIP010000232 DAOTIP010000000
VERSION     DAOTIP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43978_length_2734_cov_3.558044"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="P5138_05285"
     CDS             complement(<1..876)
                     /locus_tag="P5138_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60022.1"
                     /translation="MRIEPVAPKPGPRRNLSSRRFVVIGAVGLVLLGILFFRLWSLQI
                     VTGDKYLAEANQNRTREVRVPAPRGRILDREGDVLVDNRTSMALQLDPIEIPTQVPVR
                     RKLYARIGKVLGHDADWVRRKVRESKKNDPPGSPITLDPDVDDDIVYYLQEHQAEFPE
                     VQVNRIFVRKYPQGILAAHILGSIGEVTAEDLENNPDKDLAAGDTLGKTGIEQTYDDE
                     LRGKPGLTRLQVDSTGRIISQLRSTLPVPGESVRLSLDEKVQQAGESSLQSIGLPGAF
                     VALDVNNGQVLGLASN"
     gene            complement(873..1466)
                     /gene="mreD"
                     /locus_tag="P5138_05290"
     CDS             complement(873..1466)
                     /gene="mreD"
                     /locus_tag="P5138_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRV60023.1"
                     /translation="MIITTRTAARIGGLILLLAVLQVSFFSQVELLGTSMWILPACAA
                     IFGLLGGSLIGATVGFTFGFLGDAMTDGPLGSACLVFLAVGYLAGLYRERGDFVDRPT
                     VLVTCGLATLGSNVVLGLYTVVVGFDSSLSASLVPDLILQAFYGVLLAIPLYALIHRV
                     LRPALIHEPATRRRPADLDRWTDELDPPAGNGPEAFQ"
     gene            complement(1463..2311)
                     /gene="mreC"
                     /locus_tag="P5138_05295"
     CDS             complement(1463..2311)
                     /gene="mreC"
                     /locus_tag="P5138_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRV60024.1"
                     /translation="MYRKQVRRRRAVLIGLIVLGFVLLTFTFGSGSGGLGGAVGTVFD
                     PITNGASRALKPARDLVNWFDETMNARGERDRLQRDLEEARGQAVAGQVAMEENRQLR
                     QLVGLKKAGKIPDAYSPVTVNVTGRSPTVWYTTVNVNAGSSDGVAVNDPVISGDGLVG
                     RISSVSRSGSVVSLISDSNSAVSAMILPGGAQGVIRAKVGDPRTLVLEFLDETKNVTS
                     GQTVVTSGWRGDGLSSLYPRGLPVGEITRAPIDEREAIQSVELRPFADLRNLDTLQIL
                     TGGNRG"
     gene            complement(2479..>2734)
                     /locus_tag="P5138_05300"
     CDS             complement(2479..>2734)
                     /locus_tag="P5138_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRV60025.1"
                     /translation="GLAIVGPEDERVRSGVAESTVRFRELGSAEIGRYLASGEWRDRA
                     GGYAIQGRGSALVAGVEGDIANVIGLPVGLLLNLAPELDL"
BASE COUNT          482 a          889 c          901 g          462 t
ORIGIN      
        1 gttcgaagcc aggccgagaa cctgtccgtt gttgacgtcc agtgcgacga aagcgccggg
       61 caggccgatg ctctgaagcg aggactcccc tgcctgctga accttttcgt caagcgaaag
      121 tctcacgctt tcgcctggca ccggcagggt cgacctgagc tggctgatga tccggccggt
      181 cgaatcgacc tgcaggcggg tcaggccggg cttgcccctc agttcgtcgt cgtaggtctg
      241 ttcgatgccg gtcttgccga gcgtgtcccc ggcagcgaga tccttgtccg ggttgttttc
      301 gaggtcctcg gcggtgacct ccccgatgct gccgagaatg tgggccgcga ggatgccctg
      361 cgggtatttc cggacgaaga tccggttcac ctggacctcc gggaactctg cctgatgctc
      421 ctgcaagtag tagacgatgt cgtcatccac gtcgggatca agcgtgatcg ggctcccggg
      481 cgggtcgttc ttcttcgatt cccggacctt gcggcgaacc cagtcggcgt catggccgag
      541 caccttgccg atcctggcgt agagcttcct gcgaaccggg acctgggtcg ggatctcaat
      601 cgggtcgagc tggagggcca tgctggtccg gttgtcgacc aggacatcgc cctcgcgatc
      661 caggatcctc ccacggggag cgggcacgcg gacctcgcgg gtccggttct ggttggcctc
      721 ggccaggtac ttgtcgccgg tcacgatctg cagcgaccag aggcggaaga agagaatccc
      781 cagcaggacg agtccgaccg cgccgatcac gacgaaacgg cgcgaggaaa ggtttcgcct
      841 ggggccgggt ttgggggcta cgggttcgat tctcattgaa atgcctcggg gccgtttccg
      901 gccggcggat ccagctcatc ggtccagcga tccaggtctg cgggccgccg gcgggtggcc
      961 ggctcgtgaa tcagcgctgg tctgagaaca cgatggatca gcgcgtagag cggaatcgcc
     1021 agaagaacac catagaaggc ctgaaggatg aggtcgggca ccagcgacgc gctcagcgac
     1081 gagtcgaagc cgactaccac cgtgtagagg cccagaacca cgttcgagcc gagcgtcgcc
     1141 aggccacagg tgacaagcac ggtgggtcgg tcgacgaagt cgcccctttc ccggtagagg
     1201 ccggccaggt agcccacggc gaggaagacc agacacgcgc tgccaagcgg tccgtcggtc
     1261 atcgcgtccc cgaggaagcc gaatgtgaaa ccgaccgtgg cgccgatcag gctgccgccc
     1321 agcaatccga aaatcgcggc gcaggcgggc agtatccaca tcgaggtccc gagcagctcg
     1381 acctgggaga agaacgacac ctggaggacg gccaaaagga gaatcaggcc gccgatccgg
     1441 gccgccgttc gagtcgtgat gatcatccgc gattgccccc ggtcaggatc tgcagggtgt
     1501 cgaggttgcg caggtcagcg aaaggccgga gctcgaccga ctggatcgcc tcccgctcgt
     1561 cgatcggtgc ccgggttatt tcgccgaccg gcaggccgcg gggataaagc gacgagagtc
     1621 cgtctccgcg ccagcccgag gtaacgacgg tctgtccgct ggtcacgttc ttggtctcgt
     1681 cgaggaattc gagcacgagc gtccgggggt ctcccacctt ggcgcggatc acgccctggg
     1741 caccgcccgg gaggatcatc gcgctcaccg cgctgttgct gtcggaaatc aggctgacga
     1801 ccgagcccga gcggctgacc gagctgatcc ggccgaccag gccgtcgccg ctgatcaccg
     1861 ggtcgttcac cgcaacgcca tccgaggacc cggcgttcac gttcaccgtg gtgtaccaga
     1921 cggtcggaga acgaccggtc acgttgaccg tgaccgggct gtaggcgtcg ggaatcttgc
     1981 ctgccttctt caggccgacg agctgacgca gttgccggtt ttcctccatc gccacctgac
     2041 cggccactgc ctggccccgg gcttcctcga gatcccgctg cagccggtcc cgctcgccac
     2101 gggcattcat ggtctcgtcg aaccagttga ccagatcccg ggccggcttg agcgcccggg
     2161 acgccccgtt cgtgatcggg tcgaaaacgg tcccgaccgc tccgccgagc ccgccagaac
     2221 cgctgccgaa agtaaaagtg agcagcacga aaccgagcac gatcaggccg atcagtacgg
     2281 ctcgtcgacg tcggacttgc ttgcggtaca cgttggtctc aggctcggtt cgaagaaagg
     2341 ggcaggctca ggatgcccgg agcggattcg ccccggacag gcgactttct cacctccgcg
     2401 agaaggccgt atatacggac aggtggctgc gggaaggatt gcagcgttcg ggaaccctag
     2461 cgggactggc tcacgggttc ataaatccag ctctggagcc aaattcagca ataaacccac
     2521 ggggagaccg atcacgttgg cgatgtcgcc ctcgaccccg gctacgaggg ccgatcccct
     2581 gccctgaatc gcgtacccgc cggccctgtc ccgccattcg ccgctcgcga ggtatcggcc
     2641 gatctcggcg gagccgagtt cccggaaccg gaccgttgac tcggccactc cgctgcgtac
     2701 ccgctcgtcc tccgggccga cgatcgcgag accg
//
LOCUS       DAOTIP010000233         2730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44059_length_2730_cov_2.962991, whole genome shotgun sequence.
ACCESSION   DAOTIP010000233 DAOTIP010000000
VERSION     DAOTIP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44059_length_2730_cov_2.962991"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="P5138_05305"
     CDS             complement(<1..1630)
                     /locus_tag="P5138_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV60026.1"
                     /translation="MSKFFGGVAAATARRPWPVLIIAVILSVVSIWAASGMGTQNVTD
                     AFFDRDSTAYRQTAEADREFGTDPVVIMAKGPLVETLSTYNLNRLATLETCLAGRIKQ
                     GRGRLFRICRDIADLHPAKVIAGPATFLGRAVAGITEVYNRQIKRLASLPDTPAGRAE
                     RNRLLAMAAQVVSRYGLTQAPSLEDPTFVRRVVFGEGGVRAGPKPKLNYLFPSSDAAQ
                     IVMRLRSDLTEKQRSRAIDLIKQAVNDPSIKLRESDLVVSGSPVVFAGLNDELPVRVL
                     VLAAVAVLLMSLALFFAFGSIWRLLPLGLALGGLALAAGVLRLSGGEFSLASLGAAPI
                     LIGLTVDYAVQLQARFDETEKLPAPQAAGEAAMLGVPMIATACVATAAGFGALAFSSL
                     PLVSQFGLLLGGGVLICFLFTFLTCFALLAMRGPRRPDQRHGRVITFARTVVKPVLAT
                     AITAPGRVLLLSILLGAIGWAVSTQSEVRSEISQLLPSRAGVVQDLLDVEDTTGASGE
                     IDLIVRAPDVTSPAVVAWTDQVRNRILEQSGYGGE"
     gene            complement(1632..2621)
                     /locus_tag="P5138_05310"
     CDS             complement(1632..2621)
                     /locus_tag="P5138_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60027.1"
                     /translation="MEAADEAVASDDSIAEGEQQLEDAESAQLAVTDEPEPSNGKGFV
                     ATDGAVLGKDFVIPGVGPGDNDPYKWHTGPKQVTTNNAIEIVDVVKQFGRTRILNGLN
                     LGLPDDQISMVLGPSGTGKSVLIKHIVGLLYPDHGDVIVKGDSVPDLSDDDLFEMRKK
                     FGLLFQDGALFGSMNIFDNVAFPLRQHTDKGEDEIEHICERRLREVGLGEAHFKMPNE
                     LSGGMRKRAGFARALVLDPEIVMFDEPDSGLDPVRTALLCELIKEVHAESGGCYMVIS
                     HDLGTARRIADFIAVLWKGRIVESGPKDELFESDNEFVHQFLQADVVGPLAMD"
BASE COUNT          467 a          889 c          920 g          454 t
ORIGIN      
        1 cctctccccc gtatccggac tgttcgagga tccggttgcg gacctgatcg gtccaggcaa
       61 cgaccgccgg gctggtcacg tccggagccc tgacgatcag gtcgatttcg cccgatgccc
      121 cggtcgtgtc ctcgacgtcg agcaggtcct gcaccacccc ggcgcgggac ggcaggagct
      181 ggctgatctc gctacggact tccgactggg tgctaaccgc ccagccgatc gcaccaagca
      241 ggatcgagag cagcagcacc cgtccgggcg cggtgatcgc ggtggccagg acgggcttga
      301 ccaccgtccg cgcaaaggtg atgacgcggc cgtgtcgctg atccggccgc cgcggcccgc
      361 gcatcgccag cagggcaaag caggtcagga aggtgaaaag gaaacagatc agaaccccgc
      421 caccgagcag cagcccgaat tgcgagacca ggggcaggga agagaacgcg agggccccga
      481 agccggccgc ggtcgccacg caggcggtag cgatcatcgg cacgccgagc atcgccgcct
      541 caccggcagc ctgaggcgcc ggcaacttct ccgtctcgtc gaaacgggcc tgcagctgga
      601 ccgcgtagtc gacggtcagg ccgatcagga tcggggcagc accaagcgac gccagcgaga
      661 actctccccc cgacaggcgc agcacgccgg ccgcgagtgc gaggccgccg agggccaggc
      721 cgagcggcaa gaggcgccag atcgaaccga acgcgaagaa gagggcgagg ctcatcagca
      781 gcaccgccac cgcggcgagg accagcaccc gcaccggcag ttcgtcgttc agtccggcaa
      841 agacgaccgg cgatccggac acgaccagat ccgactcccg gagcttgatc gaggggtcgt
      901 tcaccgcctg tttgatgaga tcgatcgcgc gcgatcgctg cttttcggtc aggtcgctgc
      961 gcaggcgcat cacgatctgg gcggcgtcgg acgaggggaa caggtagttc agcttcggtt
     1021 tcgggccggc ccggaccccg ccctcgccaa agaccaccct gcgcacgaag gtggggtcct
     1081 cgaggctggg cgcctgggtc agcccgtacc tggaaacgac ctgggcggcc atcgccagca
     1141 gccggttcct ttccgccctt cctgcagggg tatcgggcag cgatgccaga cgtttgatct
     1201 ggcggttgta gacctcggta atgccggcaa ctgcacggcc gaggaaggtc gccggcccgg
     1261 cgatcacctt ggccgggtga aggtcggcta tatcgcggca gatccggaac aggcggcccc
     1321 tgccctgctt gatccttccg gcgaggcagg tctccagggt cgcgaggcgg ttcaggttgt
     1381 aagtgctgag ggtctcgacc agcggaccct tggccatgat cacgaccggg tcggtcccaa
     1441 actcgcggtc agcctcggcg gtctgacggt aggcggtcga gtcccggtcg aagaaggcat
     1501 cggtcacgtt ctgggtaccc atgccgctcg cggcccagat cgagaccacg ctgaggatca
     1561 ccgcgatgat cagcaccggc cagggccggc gggcggtggc cgcggcgaca ccgccgaaga
     1621 acttcgacat ctcagtccat cgcgagcggg ccgacgacgt cggcctgcag gaactggtgg
     1681 acgaactcgt tgtccgattc gaacagctcg tccttcggtc cgctttcgac gatccggccc
     1741 ttccagagca ctgcgatgaa gtcggcgatc cggcgggcgg tgccgaggtc gtggctgatc
     1801 accatgtagc agccgccgct ctcggcatgg acttccttga tcagttcgca gagcagagcg
     1861 gtgcggaccg gatcaaggcc ggagtccggc tcgtcgaaca tgacgatctc cgggtcgagc
     1921 accagcgccc gggcgaaccc ggcgcgcttg cgcatgccgc cggagagctc gttcggcatc
     1981 ttgaagtggg cttcgccgag accgacctcg cgcaggcggc gctcgcagat gtgctcgatt
     2041 tcgtcctcac ccttgtctgt gtgctgccgg agcgggaagg cgacattgtc gaagatgttc
     2101 atcgagccga agagggcgcc gtcctggaag agcagcccga acttcttgcg catctcgaag
     2161 aggtcgtcgt cgctgaggtc cggaaccgag tcacccttca cgatcacgtc tccgtgatcg
     2221 gggtagagca gaccgacgat gtgcttgatc agcacgctct tgccggtgcc cgaggggccg
     2281 agaaccatcg aaatctgatc gtcggggagt cccaggttga gaccgttgag aatgcgggtg
     2341 cggccgaact gcttgaccac gtcgacgatc tcgatcgcat tgttggtggt cacctgtttg
     2401 ggtccggtat gccacttgta cggatcgttg tcaccggggc ccactccggg gatgacgaag
     2461 tccttgccca gcacggcgcc gtcggtggcg acgaatccct tgccgttgga aggttccggc
     2521 tcatcggtta ccgccagctg ggccgactcg gcgtcctcga gttgctgctc gccttcggcg
     2581 atcgaatcgt cggaagcgac ggcctcgtcc gcggcctcca tctcaccggc gatttccggc
     2641 tcttcggtct tttcgatcgc ggcctcgacc tcgggagagt tgatcggagt ctcgtccgat
     2701 gcctgcggat ccggtgtttc ttctgtgtcg
//
LOCUS       DAOTIP010000234         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44339_length_2717_cov_2.352367, whole genome shotgun sequence.
ACCESSION   DAOTIP010000234 DAOTIP010000000
VERSION     DAOTIP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44339_length_2717_cov_2.352367"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5138_05315"
     CDS             <1..464
                     /locus_tag="P5138_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRV60028.1"
                     /translation="DLRDNGGGLLEEAVFTSSVFLDEGDVVVETRSRSQGNTVYKAVK
                     GKVDSPPIVVLVNRNTASAAEILAAALQTDMDTPVVGTRTFGKGLFQQVLPLDNGGAL
                     DLSVGEFLTADGVSLAGKGLKPDVYAPLPKNATRDVQLNRAFQVLAGETDG"
     gene            457..>1001
                     /locus_tag="P5138_05320"
     CDS             457..>1001
                     /locus_tag="P5138_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRV60029.1"
                     /translation="MADRRQDPGRGTRRVALLERKGRFTSARPLFERGPAVALERKAR
                     TREGQIAWVEFGPGRARLIRDLGRIDNASEVCEALTLDRLGRRGFSRAVEEAAEGAAA
                     SPEEASRQDLTGLPTFTVDPATARDFDDAVSARREGDGVRLWIHIADVSAFVRPGSLL
                     EKEAFLRANSTYVPTSVEPMLP"
     assembly_gap    1002..1011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1012..2203
                     /locus_tag="P5138_05325"
     CDS             <1012..2203
                     /locus_tag="P5138_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRV60030.1"
                     /translation="HSLSSGVCSLSPGEDRLAVTTEMVLSGSGEVTRVSFYRSRIRSD
                     ARLDYEQLDRIFLDREQAPEPVAGPLALAREAAAVLADRRRGSSLEVNKAEPSFEFDG
                     EGHVVRAVAETATEAHRLIEQLMVLTNEQVAELTSSRGIPSVYRVHEMPDPDRVERML
                     AQLASLDLPAPPVPAGMGPTAAGEIAVEASRSVSAEAARRGHGGEAYSSLILRSLKPA
                     RYLEKNLGHAGLGSDAYCHFTSPIRRFPDLFVHRALLSAVGGGEEGPSTGEAAEAALH
                     ASEREREAMILERDADDVCAAFLLERELFEEGYEKRFEGEVSGVIRSGAFVRFGGRLG
                     DVYEGFVPARRIRGERFELNEEETSLVGSKTGRKVSLGDEIAVNVEKIDAPAGRVDLD
                     PSDG"
     gene            2216..2701
                     /gene="smpB"
                     /locus_tag="P5138_05330"
     CDS             2216..2701
                     /gene="smpB"
                     /locus_tag="P5138_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRV60031.1"
                     /translation="MAKKGKKRAPASGDVATNRRARQKFEIVEKFEAGIALLGTEVKA
                     LREGNAQMGDAYAVVEDGEVWLRNLHIPHYAPATINNHDPERPRKLLLHRAEIERLIG
                     TTQQKGLTLVPTRIYFKGPRAKVELGLARNKEGRDRRREIADRDVKRDIEREFKGRYR
                     S"
BASE COUNT          475 a          850 c          946 g          436 t
ORIGIN      
        1 tcgatctccg tgacaacggc ggtggcctgc tcgaggaggc ggtgttcaca tcgagcgttt
       61 tcctggacga gggtgacgtg gtggtggaga caaggtcccg cagccagggc aacaccgtct
      121 acaaggcggt caagggcaag gtcgacagcc ccccgatcgt cgtcctcgtc aaccgcaaca
      181 cggcctcggc cgcggagatc ctcgccgcgg cgctgcagac ggacatggat accccggttg
      241 tcggcaccag gacttttggc aagggtctgt tccagcaggt acttccactc gacaacggcg
      301 gcgccctgga cctgagtgtc ggggagttcc tcaccgccga cggtgtatcg ctcgccggga
      361 agggcctgaa gcccgacgtg tatgcgcccc tgccgaagaa cgcgacccgg gacgtccagc
      421 tcaacagggc attccaggtg cttgcgggcg aaacggatgg ctgaccgccg ccaggacccc
      481 ggtcgcggca cccggagagt cgccctgctt gaacgaaaag gtcgcttcac ctcggcccgg
      541 ccgctctttg aacgcggtcc cgcggtcgct ctcgagagga aggcccgcac acgggaaggg
      601 cagatcgcct gggtcgagtt cgggccgggg cgggcccgtc tgatccgcga cctgggccga
      661 atcgacaacg catcggaggt ctgtgaggcc ctgacgcttg atcggctcgg tcgccgcggt
      721 ttctcccgtg cggtcgagga ggcggccgaa ggggccgccg cttctccgga ggaagcgtcc
      781 cgccaggatc tgaccggact cccgaccttc accgtggacc cggccaccgc ccgcgacttt
      841 gacgacgccg tttctgcccg ccgcgagggg gacggggtgc ggctctggat tcacatcgcc
      901 gacgtatcgg ctttcgtccg cccgggctcg ttgctggaga aggaggcgtt cctccgcgcc
      961 aacagcacct atgtcccgac ctcggtcgaa ccgatgcttc cnnnnnnnnn nccacagtct
     1021 cagctccggg gtctgttccc tgtcccccgg ggaagaccgg ctcgccgtta caacggagat
     1081 ggtcctctcc gggtcggggg aggtgaccag ggtttcgttt taccgcagcc gcatccgttc
     1141 cgacgccaga ctcgactatg agcagctcga ccgcatcttc ctcgaccggg agcaggcgcc
     1201 ggagccggtg gccgggccgc tcgcgcttgc ccgggaggcc gctgctgtcc tggccgaccg
     1261 gcgccgtggt tcatcgctgg aggtgaacaa ggccgagccc agcttcgagt tcgacgggga
     1321 gggtcatgtg gtgagggcgg tggccgagac ggccacggaa gcccaccggc tgatcgagca
     1381 gctgatggtg ctgaccaacg agcaggtagc ggaactgacc agcagccggg gcatcccttc
     1441 cgtgtaccgg gtgcacgaga tgcccgaccc ggaccgggtc gagcggatgc ttgcccagct
     1501 ggcctcactc gatctgccgg cgcctccggt tcccgccggc atgggaccca cggcggccgg
     1561 tgagatcgcg gtcgaggcat cacggtcggt ctccgcggaa gcggcccggc gggggcatgg
     1621 cggcgaggcg tacagctcgc tgatcctgcg gtcgctgaaa ccggccagat acctggagaa
     1681 gaacctcggc cacgccgggc tgggcagcga tgcctactgt catttcacct cgccgatccg
     1741 tcgcttcccg gacctgttcg ttcaccgggc gctgctctcg gcggtcgggg gcggggagga
     1801 ggggccatcg accggcgagg cggcagaggc agcccttcac gcgtcggagc gggagcgcga
     1861 agcgatgatt ctggaacggg atgccgatga tgtctgcgcc gcgttcctgc tcgaacggga
     1921 actcttcgag gaggggtacg agaagcgctt cgaaggggag gtctccgggg tgatccgctc
     1981 cggtgccttt gtcaggttcg gcggacggtt gggagacgtg tacgagggct tcgtgccggc
     2041 gcgccggatc cggggcgaga ggttcgagct caacgaggaa gaaacgtccc tggtcggttc
     2101 aaagaccgga cgcaaggtca gcctcggcga cgagatcgcg gtgaacgtcg aaaagatcga
     2161 cgcaccggcc ggccgggtcg atctggatcc ctcagacgga taaattccct ctttcatggc
     2221 gaagaaggga aagaagcggg ccccggcctc cggtgacgtt gcgaccaacc ggagggccag
     2281 gcagaaattc gagatcgtcg agaagttcga ggccggcatt gccctgctcg gaaccgaggt
     2341 caaggcgctg agagaaggga atgcccagat gggcgatgcc tacgcggtgg tcgaagacgg
     2401 cgaggtctgg ctccgcaacc ttcacatccc ccactacgcc ccggcgacga tcaacaatca
     2461 cgatccggag cgcccgcgga aacttctgtt gcatcgggcg gagatcgaga ggttgatcgg
     2521 cactacccag cagaagggcc tcacgctggt tccgacccgc atctatttca aggggccaag
     2581 ggcaaaagtc gaacttggcc tcgcccgaaa caaggagggc cgggaccggc ggcgtgaaat
     2641 tgccgatcgc gatgtgaaac gagatataga aagggaattc aaggggcggt atcgttcctg
     2701 acctgatgat gaagctt
//
LOCUS       DAOTIP010000235         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44360_length_2716_cov_3.033822, whole genome shotgun sequence.
ACCESSION   DAOTIP010000235 DAOTIP010000000
VERSION     DAOTIP010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44360_length_2716_cov_3.033822"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1613
                     /locus_tag="P5138_05335"
     CDS             426..1613
                     /locus_tag="P5138_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRV60032.1"
                     /translation="MNTTLVNHGRKALTVKVALAALLAACVALVFGANNAKAMNAGDP
                     FNAEFQYAGLNVDVTLAQLDEMVLDGTGDSLLISGTYSNSNGDLTLPKNTGLTFPQIL
                     LPLTEGVDLNANIELASDGTGNYNPSTGVMSLELPVALTIGVSDVSALPIPGLGSGPL
                     TCKFSPINISLSTENGWPHDGYAFADPATVENGALAGAWRSKPPVTAVEGAQATCDLI
                     GGVLKDVGGLWLANSTTPLSSMPAATSSKPSVETCEEHGQVGTFPDCETPVTECDPGF
                     EGTPPNCTKILSDGKVGSLTITKKATVKAGKSVKIKVTVKNTGEKTLKTKLTLSSSNK
                     KVAASPKSVSLSIAGGKSITKTITVKASKKAKGKATITAKAGGKSAKSAVTVKAAKKK
                     KKK"
     gene            1753..1825
                     /locus_tag="P5138_05340"
     tRNA            1753..1825
                     /locus_tag="P5138_05340"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            complement(1927..>2716)
                     /locus_tag="P5138_05345"
     CDS             complement(1927..>2716)
                     /locus_tag="P5138_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRV60033.1"
                     /translation="IEGSRGKIVVHKWAPVGEPTYIVLLAHGYAEHALRHAGLAEKFE
                     ADGAVVYAPDHRGHGRSEGEPGLVDEAQPLIDDFEKVYDLAREEHPDLPILLVGHSMG
                     GLLATLFIQEAGRSVDAMVLSSPLLGGNPALVALLDLEEIPEVPIDPTVISRDENEQQ
                     AYLDDPMIYHGPFRRGTLEGLVAGLDRARAGGGFGELPVLWIHGEGDMLVPYDLTAET
                     MEVLSGPGTEAKSYPEARHELFHETNREEVYSDVFDFVGREIES"
BASE COUNT          523 a          855 c          868 g          470 t
ORIGIN      
        1 cgaggacatg aaggaatgga ccggttgcat cgccggagcg cttacccgcc atgagttcga
       61 atccggtctt gcaggggccg gcttcgtgga cattcagatc gaggagacgc acagagtcca
      121 cgaccaggcg tcatcggcaa tagttcgcgc gacgatgcca gagtgagctt tcaccggggc
      181 ctctgaacgg caccgggagg cagccgggaa tccagaaacc gattgtttgc gaaatgttgc
      241 cggtttctgt atgctgccgc cattactggt cctgtgtgat cgctgtcact attgcagcga
      301 ccgggcaggg cttgacaggg aggacggcca ttcggtcgtc gggaaatcgt caagagcagg
      361 gaccccgctg catctaggga gacaacgaac cggccggacc tgagggagaa ggccacggag
      421 gaaagatgaa caccactctc gtaaaccatg gccgcaaggc cctcaccgtg aaggtggctc
      481 tggccgcgct tctggccgcg tgtgtggcgc tggtcttcgg tgcgaacaac gccaaggcca
      541 tgaacgcggg tgacccgttc aacgccgagt tccagtacgc cggcctgaac gtcgacgtga
      601 ctctggccca gctcgatgaa atggtgctcg acggcaccgg cgattcgctg ctgatctcgg
      661 gtacctacag caactccaac ggagatctga ccctgccgaa gaacacgggt ctgaccttcc
      721 cgcagattct gctgccgctg accgagggcg ttgacctcaa tgccaacatt gagctcgctt
      781 cggacggtac cggcaactac aacccgtcca ccggcgtcat gagcctggag cttccggttg
      841 cgctcacgat cggtgtcagc gacgtatcgg ccctgccgat tccgggtctc ggttccggcc
      901 cgctgacctg caagttctcg ccgatcaaca tcagcctctc gaccgagaat ggctggccgc
      961 atgacggtta cgcgttcgcc gatccggcga ctgtcgagaa tggtgcgctc gcgggtgcct
     1021 ggcgttcgaa gcctccggtg accgcggtcg aaggcgccca ggcgacctgt gacctgatcg
     1081 gtggcgtgct caaggacgtc ggcggcctct ggctcgccaa cagcaccacg ccgctcagct
     1141 cgatgcccgc tgcgacgtcg agcaagccca gtgtggagac ctgcgaggag cacggccagg
     1201 tcggtacgtt ccccgactgc gaaaccccgg tcaccgagtg tgacccgggc ttcgagggaa
     1261 ctccgcccaa ctgcaccaag atcctgtccg acggcaaggt cggcagcctg acgatcacca
     1321 agaaggcgac ggtcaaggcc ggcaagtcgg tcaagatcaa ggtcacggtc aagaacaccg
     1381 gtgagaagac cctgaagacc aagctgacgc tgtcttcgag caacaagaag gttgcggctt
     1441 caccgaagtc ggtctcgctc agcattgccg gcggcaagtc gatcaccaag acgatcaccg
     1501 tcaaggccag caagaaggcc aagggcaagg cgaccatcac ggccaaggcc ggtggcaagt
     1561 cagccaagtc cgcggtcacg gtcaaggccg ccaagaagaa gaaaaagaag tagcactgag
     1621 gcgacttctt tcggctcagc cgaagcattg agacaaaggg ccccggaaaa atccggggcc
     1681 cttttctttg gcttgcacgg ccgcggtttc aaagcgtcgc aaggcccgtt cccgatagcc
     1741 tgtcgagtcc ttggcgaggt agctcagttg gtagagcaca cgactgaaaa tcgtggtgtc
     1801 gccggttcga tcccggccct cgccatatga ccgcacgtca tccggtcttc gccggatgac
     1861 cacagaaacc cgccccaccc actcgcacta actgatggtt gggcggggct ctgctggatg
     1921 gtgggactag ctttcgatct cccggcctac gaagtcgaag acgtcggagt agacctcttc
     1981 gcggttggtt tcgtggaaga gctcgtggcg ggcttccgga taggacttcg cctcggttcc
     2041 cggacccgac aggacttcca tcgtttcggc ggtcaggtcg taggggacca gcatgtcgcc
     2101 ctccccgtga atccacagca ccgggagctc gccgaatccc ccgcccgcgc gggcccgatc
     2161 cagtccggcg acaagtccct cgagggtgcc tcggcggaac gggccgtggt agatcatcgg
     2221 gtcgtcgagg taggcctgct gctcgttctc gtcccggctg atcaccgtcg ggtcgatcgg
     2281 gacctccggg atctcctcga gatccagcag cgcgaccagt gccgggttgc cgccgagcag
     2341 cgggctggag aggaccatcg cgtcgaccga ccggccggcc tcctggatga acagggtggc
     2401 gagcagtccg cccatcgaat ggccgaccag caggatcggc aggtcgggat gttcttcccg
     2461 ggcgaggtca tagaccttct cgaaatcatc gatcaggggc tgggcttcgt cgaccagacc
     2521 gggctcgccc tccgaccgcc cgtgaccgcg gtggtccggg gcatagacga cggcgccgtc
     2581 ggcttcgaac ttctcggcga gtccggcgtg acgcagggca tgttcggcgt agccgtgggc
     2641 cagcagcacg atgtaggtgg gttcgccgac cggcgcccac ttgtgaacga cgatcttgcc
     2701 cctgctgccc tcaatg
//
LOCUS       DAOTIP010000236         2709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44522_length_2709_cov_2.840995, whole genome shotgun sequence.
ACCESSION   DAOTIP010000236 DAOTIP010000000
VERSION     DAOTIP010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44522_length_2709_cov_2.840995"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="P5138_05350"
     CDS             complement(<1..191)
                     /locus_tag="P5138_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60034.1"
                     /translation="MTSPNPRITLCGRLSVSWDGEEIGESLPGRQGRLLFAFLALNRR
                     RPVRRDELVEALWADEGLP"
     gene            254..1552
                     /locus_tag="P5138_05355"
     CDS             254..1552
                     /locus_tag="P5138_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60035.1"
                     /translation="MSTTSLINLLPAAARIAPETPEDVAFAFAEARRTGRKLVVFSTG
                     HGIPPIGDTSDRILLDMGAFDQVEIDPSARVARIGGAVQWSDLVSAANRHGLTAPFGS
                     AASVGVTGYMMGGGIGFMSRHLGLGSSAIRSAELVAPDGRILHINAKSDPDLFWALRG
                     GGGGFGVITSLEVELTPVTAMSGGVLGWGIEDAERVLRTWSTWTERLPDDVTSAVRFM
                     HPPEGPSLTVVVVTAPRDEADLSEVLSPLTDLQPVLNTVRNTDPEAFINEYTDPEIDP
                     EEPPHAFEHVLFDRLPGDACQMVAEFARPADGCGAMMIEIRHLGGALATPAASGGATD
                     MVDGEYCFFVVGQLEAAEGMAHAVDVLSDYGRGRTYFNFMPRRTGRQTAYRQEAIHRL
                     ADLYEAMDPEGLLDHPHPISRTASVVDEAAEGPALAKLPG"
     gene            1659..>2709
                     /locus_tag="P5138_05360"
     CDS             1659..>2709
                     /locus_tag="P5138_05360"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60036.1"
                     /translation="MSLRPTIDLLLEDEQISRLSAEVRDAVAAREAGSGSGVVGGSVR
                     AEVSASLRPALIAALLEDDLGLEDRPALIVTPDDRSAREMSAAMGAFLGDRVVRSYPS
                     RGTGYGSQVTPPPHLTGLRIDALDSFGREEEKPPIVVASAIALAESVPDSSLRPAGFR
                     LAVGEEHEPSQIAADLVAAGYERVDQVQERGQFAVRGGILDVFSATEDRATRIDFFGD
                     EIESMRWFSTFTQRSLGQAESIELAPAAELDADHRELAELAIAEARENETDMDLTEAL
                     PLDSFRAPLDLIPAGAAVILTADEEIEPALKDHWEDVTTAMHADDAKHLYEDVAGPLN
                     ERAALILARPGEGEES"
BASE COUNT          432 a          947 c          875 g          455 t
ORIGIN      
        1 ctcggcaggc cctcgtccgc ccagagtgcc tcgaccagtt cgtctcttct caccggccgg
       61 cgccggttca gcgccagaaa tgcgaacagg agccttccct ggcgtccggg cagggattcc
      121 ccgatttcct ccccgtccca ggagacggaa agcctgccgc agagtgtgat tcgggggtta
      181 ggggaagtca tcgcaaggtg cctgtcattt cgccgcaatc cggacgcaac ctgtgccggg
      241 gattctgcca gccatgtcaa cgacttctct catcaacctc ctgcccgcgg cggcccgtat
      301 tgcgccggaa acccccgagg atgtggcgtt cgcattcgca gaagcccgcc gtaccggcag
      361 gaagctggtg gtcttctcga ccggtcacgg aattccgccg atcggtgaca cctcggatcg
      421 catcctgctg gacatgggtg ccttcgacca ggtcgagatc gatccgtcgg cccgggttgc
      481 ccggatcggc ggcgccgtcc agtggagcga cctcgtttcg gcggccaacc gccacggact
      541 caccgccccg ttcggctcgg ccgcttcggt cggcgtgacc ggatacatga tgggtggcgg
      601 catcggcttc atgtcccgcc atctcggtct gggcagttcc gcgatcaggt cggccgaact
      661 ggtcgcgccg gacggccgca tcctccacat caacgccaag tccgatcccg accttttctg
      721 ggccctgcgc ggcggtggcg gcggcttcgg tgtgatcacc agcctggaag tcgaactcac
      781 cccggttaca gccatgtcgg gcggcgtgct cggctggggc atcgaggatg ccgaacgtgt
      841 gctgcgcacc tggtcgacct ggaccgaaag gctgccggac gacgtgacct cggccgtccg
      901 cttcatgcat ccgccggagg gtccttccct gaccgtcgtc gtggtcacgg ctccccgtga
      961 cgaggccgac ctgagcgagg ttctgagccc tctgaccgat cttcaaccgg tcctgaacac
     1021 ggtccgcaac accgaccccg aggccttcat caacgagtac acggacccgg agatcgaccc
     1081 ggaagagccg ccccatgcct tcgagcacgt gctgttcgac cggctgcctg gtgacgcctg
     1141 ccagatggtc gcggagttcg cccgaccggc cgacggttgc ggcgcgatga tgatcgagat
     1201 ccgccatctc ggtggtgccc tggccactcc ggccgcttcg ggcggggcga ccgacatggt
     1261 tgacggcgag tactgcttct tcgtcgtcgg gcagctcgaa gccgcggaag ggatggccca
     1321 tgccgttgac gtcctttccg actatggccg gggccggacc tacttcaact tcatgccgag
     1381 aaggaccggt cgccagaccg cctaccggca ggaggcgatc caccgtctcg ccgatctcta
     1441 cgaagcgatg gatcccgaag gcctgctcga ccatccccac ccgatttccc gcaccgccag
     1501 cgtcgtcgac gaagctgccg agggcccggc cctcgcaaaa ctccccggct gacagcagcc
     1561 cggcggggca accacaacgc attacacccg gtgcccggtg gtctctccct gaccaccggg
     1621 cgctttcgct ttgtcgcttc atgctttgat cgtcagccgt gtcactgcgc ccgacaatcg
     1681 acctcctgct cgaggacgag cagatctccc gcctttcggc agaggtccgc gacgccgtgg
     1741 ccgccaggga ggcgggatcc ggaagcgggg tggtcggggg gagcgtgcgg gcagaggtct
     1801 cggcgtccct gcgcccggcc ctgattgcgg ccctgctgga ggacgacctg ggactcgagg
     1861 atcgcccggc cctgatcgtc accccggatg accgttcggc ccgagagatg tccgcggcga
     1921 tgggcgcctt cctcggcgac cgcgtggttc gctcctatcc ctcgcgaggc accggatacg
     1981 gctcccaggt caccccgccg ccacatctca ccggcctgcg catcgacgca ctcgattcct
     2041 tcggccgtga ggaggaaaag ccgccgatcg tggtagccag cgcaatcgcc ctggccgagt
     2101 cggttccgga ctcttcgctc aggccggccg gcttccggct cgcggtcggc gaggaacacg
     2161 agccctcgca gattgccgcc gacctggtcg cggcgggcta cgagcgggtc gaccaggttc
     2221 aggagagagg ccagtttgcg gtccgcggcg gcatcctcga cgtattctcc gcaaccgaag
     2281 accgcgccac ccggatcgat ttcttcggtg acgagatcga atcgatgcgc tggttctcca
     2341 ctttcaccca gcgctcgctc ggtcaggccg agtcgatcga actggccccg gctgccgaac
     2401 tggacgccga ccaccgggaa ctggcagagc tggcgatcgc ggaggcccgg gagaacgaaa
     2461 ccgacatgga cctgaccgag gccctgccgc tcgacagctt ccgggcgcca ctcgacctga
     2521 tcccggcggg ggcagcggtg atcctcaccg ccgatgagga gatcgaaccg gctctgaagg
     2581 atcactggga ggacgtcacc acggcgatgc atgccgatga cgccaaacac ctttacgagg
     2641 acgtggcggg accgctgaac gaacgtgccg ccctgatcct ggccaggccg ggggaagggg
     2701 aggaatcgc
//
LOCUS       DAOTIP010000237         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44541_length_2708_cov_3.476442, whole genome shotgun sequence.
ACCESSION   DAOTIP010000237 DAOTIP010000000
VERSION     DAOTIP010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44541_length_2708_cov_3.476442"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2376)
                     /locus_tag="P5138_05365"
     CDS             complement(<1..2376)
                     /locus_tag="P5138_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV60037.1"
                     /translation="MSNPDERADRNIPDSEERRLYRGRIAEAAGRAVLENGPDPDGIW
                     AEAAGPGSYRWLWTRWIQMAPQVLGWASLLFSVAAVLGWILDVPFLTGEFLNAPAFPV
                     ASAIMIGLLATVLLLCRPGRSEAAGRMGVAIGYLTMAMALLLLFSDVVGIDPVDWGDS
                     SLMVPAFAALINVPVLALALVTIDLDAPRYRWQAILVPALGIAFLVSLLTNGYKAVLV
                     SGDTLSFSYTGALALVLVYVGYVLMRPERGLAAFLSATGPGPSMARLLVPTALLIPLT
                     MVLAEIAAEGLDAEGAAFVKGLDKILMLVLLLWMITYASRRLQRFYESWKEASSELAS
                     QANVLTRMSEGVVVMRISDTRIVLTNPQFDAMHGYRRGELIGRPIDTIAPPDLSEAEL
                     KLRSEVLHELADRGETSYESRSVRKDGSDIWSRVNGVVTTVPGHGPVLILVKYDVTEE
                     HRAKHAGRDAEMRFRQVFEQSPIGLCLVTPEGRFEKVNQSFELITGYSSGELAGMTFS
                     DITHPADLEEDLALTGELFAGERDGFSMEKRYIRKNGEVVWVYLTVAMLRDPDGHPYQ
                     ALSMVEDITERQRMSQQLQYLADHDPLTGLFNRRRFEEELNSAVTSGAGRGIAILVID
                     LDNFKFVNDSYGHTVGDRLIVRTAELLRSRLRADDTLARQGGDEFVVVLRDINPEDAL
                     ATAEELVRLVARDVRVEGAEHSAKVTASIGVAVSSQGKPVPEETLMMQADIAMYEAKD
                     AGRNGARVFHPGEDSHVTLGIDWAGRLRGALENDELLVYAQPIYRLGETGPP"
     gene            complement(2482..>2708)
                     /locus_tag="P5138_05370"
     CDS             complement(2482..>2708)
                     /locus_tag="P5138_05370"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60038.1"
                     /translation="RRRVENLEKKIEAAEAELAELEDELADPAAWSTPEKSERATRRH
                     DDARARITSLYAEWEEADQLARETAEAASV"
BASE COUNT          465 a          914 c          861 g          468 t
ORIGIN      
        1 cggcggaccg gtctcgccga gacggtagat cggctgggcg tagaccagca gttcgtcgtt
       61 ctcgagcgcg ccccgcaggc gccccgccca gtcgattccg agggtcacat gggagtcctc
      121 ccccggatgg aagaccctcg ccccgttccg gcccgcgtcc ttggcctcgt acatcgcgat
      181 gtcggcctgc atcatcaggg tctcctccgg gaccggcttg ccctgggacg agaccgcgac
      241 cccgatgctc gcggtgacct tggccgaatg ctcggcccct tccaccctca cgtcgcgggc
      301 gaccagccgg accagttcct cggccgtcgc aagggcgtct tcaggattga tgtcacggag
      361 aaccactacg aattcgtcac cgccctgccg ggccagggtg tcatcggccc taagcctgct
      421 tcgaagcaat tccgcggtac ggacgatcag ccggtccccg accgtgtggc cgtagctgtc
      481 gttcacgaac ttgaagttgt ccaggtcgat caccaggatc gcgatgccgc ggccggcccc
      541 ggaggtgacc gccgaattga gttcctcctc gaaacggcgc cggttgaaaa ggccggtcag
      601 cggatcatgg tcggccaggt actggagctg ctggctcatc cgctggcgtt cggtgatgtc
      661 ctcaaccatc gaaagcgcct ggtacggatg tccgtcgggg tcgcgcaaca tggctacggt
      721 caggtagacc cagacgacct ccccgttctt gcggatgtac cgcttctcca tcgagaagcc
      781 gtcgcgttcg cccgcgaaga gctcgccggt cagggccagg tcttcctcga ggtcggccgg
      841 gtgggtgatg tcggagaagg tcatgccggc gagttccccg gacgaatacc cggtgatcag
      901 ctcgaagctc tggttgacct tctcgaatcg cccttcgggg gtgacgaggc agagaccgat
      961 cgggctctgc tcgaacacct ggcgaaaccg catctcggcg tcgcgcccgg cgtgtttggc
     1021 cctgtgttcc tcggttacgt cgtacttgac caggatcagc acaggaccgt gaccggggac
     1081 cgttgttacg acgccgttga cgcggctcca gatatcggag ccgtccttgc ggacagacct
     1141 gctctcgtaa ctggtctccc cccggtcggc gagttcgtgg agcacttccg atcgcaactt
     1201 gagttctgcc tcggagaggt cgggaggggc gatggtgtca attggccgac cgatcagctc
     1261 gcctcgccgg taaccatgca tcgcgtcgaa ctgagggttg gtcagcacga tcctggtgtc
     1321 ggagatacgc atcacgacca ccccctcgga catcctcgtc agcacgttgg cctgggaggc
     1381 cagttccgag ctggcctcct tccagctctc gtagaagcgc tggagccggc gcgacgcata
     1441 ggtgatcatc cagagcagga ggaccagcat caggatcttg tccagaccct tgacgaaggc
     1501 ggctccctcg gcatcgagcc cctccgcggc gatttccgcc aggaccatgg tcaacgggat
     1561 gagcagggcg gtcggaacca gtagccgggc catcgagggt ccgggcccgg tcgcggaaag
     1621 gaatgccgcc agacccctct cgggcctcat cagcacgtag ccgacatata ccagcacgag
     1681 ggccagtgcc cccgtgtagc tgaacgagag cgtgtctccg gagaccagga cggccttgta
     1741 gccgttggtc agaagggaga cgaggaaggc gatcccgagg gccggaacca ggatcgcctg
     1801 ccagcggtac cggggggcgt cgagatcgat cgtgaccagg gccagggcca ggacggggac
     1861 attgatcaac gccgcgaacg ccggcaccat cagggagctg tcaccccagt cgaccggatc
     1921 gatgccgacc acgtccgaga agagcagcag gagcgccatc gccatggtca ggtaaccgat
     1981 cgccacgccc atcctgccgg cagcctccga acggcccggc ctgcacagca gcaggactgt
     2041 ggccagcagt ccgatcatga tcgcggaggc gaccggaaag gcgggcgcat tgaggaattc
     2101 acctgtcagg aagggcacgt cgagaatcca gccaagtaca gccgcaacgc tgaacagcag
     2161 cgaggcccag ccgagcacct gcggagccat ctggatccac ctggtccaga gccagcggta
     2221 gctgcccggc cccgccgctt ccgcccagat cccgtcggga tcggggccgt tctcgagtac
     2281 tgcccggccg gccgcctcgg ctatccgccc gcggtagagc ctccgctcct ccgagtcggg
     2341 aatgttccga tcggcccgct catctgggtt cgacaagtgc ttctccaagg acgactcctc
     2401 gcaagggcaa ctcctcgcct ccgaaatcta cacttgcctt tccccgtacc gggcgtcacg
     2461 aaaccgcggg acgggggaga ttcagaccga agcggcctcc gccgtctccc ttgcgagctg
     2521 gtccgcttcc tcccattcgg cgtacagcga agtgatcctc gccctggcgt cgtcgtgccg
     2581 ccgggtcgcc ctttcgctct tctcgggggt tgaccacgcc gcgggatcgg ccagttcatc
     2641 ctcgagttcg gcaagttccg cctcggcagc ctcgatcttc ttctccaggt tctcgacccg
     2701 acggcggg
//
LOCUS       DAOTIP010000238         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44569_length_2707_cov_2.559201, whole genome shotgun sequence.
ACCESSION   DAOTIP010000238 DAOTIP010000000
VERSION     DAOTIP010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44569_length_2707_cov_2.559201"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="P5138_05375"
     CDS             <1..454
                     /locus_tag="P5138_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60039.1"
                     /translation="DPDLPFLGAHLTRTIDGSLLIGPTAMIAGARDAYRVTKLRRRDL
                     GQTLRWPGTWGLMRQHWRASFNEAANSLWPSRLAGEAARLVPALARTDVEAGPAGVRA
                     QALDRDGNLIEDFLIERTGQAVHIRNAPSPAATSSLALAELIADEALA"
     gene            complement(524..1705)
                     /locus_tag="P5138_05380"
     CDS             complement(524..1705)
                     /locus_tag="P5138_05380"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRV60040.1"
                     /translation="MPEAVIVDTVRTPIGRAFKGSLAQLRPDETGAFVIDQLLERNPG
                     VDPKEVEEVFCGVGMPQGEQAYNLARVLVMLSDKLGQETTGVTVSRYCASSLDAIRHA
                     SNAVKNGEGDIYIAAGVEFVSKFNARTEIAGSIAAGEHDQNENLIGNNGQPNVYIDMG
                     VTAVNVANEYGVSRDTMDDYALRSQHLAVKSQEDGFFDREIVPVTLPDGTVINKDDGP
                     RADGGDMREKLGSLPEAFGGGGVTAGNSCPLNDGAAAVLIMSDTKAAELGLTPRAKIV
                     TSATAGNEPEKMGVAPIWAVKKLLDRAGMKIEDIDTVELNEAFAAQVIPISEQVGIPL
                     DKLNTHGGAIALGHPFGMTGARIMGTLINVMETDDHQFGIETMCVAGGQGEAMLVERL
                     N"
     gene            1830..2606
                     /locus_tag="P5138_05385"
     CDS             1830..2606
                     /locus_tag="P5138_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60041.1"
                     /translation="MTNPPRDGGPSEVPINAPQGRQQPTPGSQPTPAPAQSGRPQPSP
                     PVGQPTSPPASSSGATGNPAQASPAGQPASPGQTPASSPQGQPQAPASSQQQVRYPAQ
                     QDSRPATGQQVPPRGVPPRAVTPRTTGSGRLPAAQQRQPIDLATRLDGQRGWLAQLDK
                     SLKRRTIVALVLTCLAVGVGAAALYISLTKNSDSDRIDALQTRVEALETAAGIPSTDT
                     GSTDTGTVPETGVTLPDTGVTLPDGTTDSSGVVIPPTGGE"
BASE COUNT          410 a          959 c          903 g          435 t
ORIGIN      
        1 cgaccccgac ctccccttcc tcggtgccca cctgaccagg acaatcgacg gctccctgct
       61 gatcgggccg accgcgatga tcgccggcgc ccgcgacgcc taccgggtca cgaagctccg
      121 acgccgcgac ctcggtcaga cgctccgctg gcccggcacc tgggggctga tgcggcagca
      181 ttggcgcgcg agcttcaacg aggccgccaa ctcgctgtgg ccctcccgtc tggccggtga
      241 agccgcgagg ctcgtgccag ccttggctcg gaccgacgtg gaggcgggac cggccggcgt
      301 gcgagcccag gccctggacc gggacgggaa cctgatcgag gacttcctga tcgaaagaac
      361 cgggcaggcg gtccacatca gaaacgcccc ctcccccgcc gcaacctcat ccctggccct
      421 ggcagagctg atcgcagacg aggccctggc ctgacccggg ccacaagaaa gggcggcccc
      481 ggtcgggacc gccctttcga tgtaacagtc tggagtcggg tgactagttg aggcgctcga
      541 cgagcattgc ctcgccctga ccgccggcga cgcacatggt ctcgatgccg aactgatggt
      601 catcggtctc catgacgttg atcagcgtgc ccatgatgcg ggcgccggtc atgccgaagg
      661 gatggccgag ggcgatcgcg ccaccgtggg tgttgagctt gtcgagcggg atgccgacct
      721 gctccgagat cgggatcacc tgggcggcga aggcctcgtt gagctcgacg gtgtcgatgt
      781 cctcgatctt catgccggcc cggtcgagca gcttcttgac ggcccagatc ggggcgacgc
      841 ccatcttctc cggctcgttg ccggcggtgg cgctggtgac gatcttggcg cgcggggtca
      901 ggccgagctc ggccgccttg gtgtcgctca tgatcagaac cgccgcggcg ccgtcgttca
      961 gcgggcagga gttgccggcg gtgacgccgc ctccgccgaa ggcctccggc aggctgccga
     1021 gcttctcgcg catgtcgcca ccgtcggcgc gggggccgtc gtccttgttg atcacggtgc
     1081 cgtccggcag ggtcaccggc acgatctcgc ggtcgaagaa gccgtcctcc tgcgacttca
     1141 cggcgaggtg ctgcgagcgc agggcgtagt cgtccatcgt gtcgcggctg acgccgtact
     1201 cgttggcgac gttgaccgcg gtcacgccca tgtcgatgta gacattgggc tggccgttgt
     1261 tgccgatcag gttctcgttc tgatcgtgct cgccggccgc gatcgacccg gcgatctcgg
     1321 tgcgggcgtt gaacttgctg acgaactcga cgccggcggc gatgtagatg tcaccctcgc
     1381 cgttcttgac cgcgttcgag gcgtgacgga tcgcgtcgag gctcgaggcg cagtaacgcg
     1441 agacggttac accggtggtc tcctgaccga gcttgtccga gagcatcacg aggacgcggg
     1501 ccaggttgta ggcctgctcg ccctgcggca taccgacgcc acagaagacc tcttcgacct
     1561 ccttggggtc cacgccgggg ttccgttcga ggagctggtc gatcacgaag gcgccggtct
     1621 cgtcggggcg gagctgggca agcgagccct tgaaggcgcg tccgatcggg gtgcggactg
     1681 tgtccacgat cactgcttca ggcatgcgaa gtctttctcc ttgggtttat ggggccggga
     1741 cgtgagggca cccggcgctg catgacggat aaatgccaca ggacagcgta ttgaactgtg
     1801 gaaatgcggg acgggtagat tgccctgcga tgaccaatcc gcctcgagac ggcggccctt
     1861 ccgaggtgcc gatcaacgct ccgcagggcc gccagcaacc cacgccgggc agccagccga
     1921 cgcctgcccc ggcccagtcc ggtcgtcccc agccctcacc cccggtcggg cagccgacct
     1981 ccccgccggc ctcttcttcc ggcgccaccg gcaaccccgc ccaggcctcc ccggccgggc
     2041 aaccggcttc cccgggccag acaccggcct cctctcccca ggggcaacca caggcaccgg
     2101 cctcttcaca gcagcaggtc cggtatcccg cccagcagga ctcccggcct gcaaccggtc
     2161 agcaggtgcc gccccgcggc gtcccgcccc gggccgtgac tccccggacc accggctcgg
     2221 gccggttgcc ggcagcccag cagcgccagc cgatcgatct tgcaacccgg ctcgacggcc
     2281 agcgtggctg gctcgcgcaa ctggacaagt cgctgaagcg gcgcaccatc gtggccctgg
     2341 tcctgacctg cctcgcggtc ggagtcggag ccgccgcgct ctacatctcg ctgaccaaga
     2401 actcggacag cgatcgcata gatgccctgc agaccagggt cgaagcgctg gagaccgcag
     2461 ccgggatccc gtcgaccgac accgggtcaa cggacaccgg caccgttccg gaaaccggcg
     2521 tcaccctgcc cgatacgggg gtcacgctgc ccgacggcac aactgactcc tcgggggtcg
     2581 tcattccccc gactggcggc gagtaggtcc gggctgacgg agggtggctt gaccggcaat
     2641 gagtccgaat ccgttcttta tggcgagccc tcgccgggcg tccgactgat cacgatcaac
     2701 cgtccgg
//
LOCUS       DAOTIP010000239         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44678_length_2701_cov_3.925548, whole genome shotgun sequence.
ACCESSION   DAOTIP010000239 DAOTIP010000000
VERSION     DAOTIP010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44678_length_2701_cov_3.925548"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="P5138_05390"
     CDS             complement(<1..350)
                     /locus_tag="P5138_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRV60042.1"
                     /translation="MSAVDQIRVDAQAALKDGDRKRASALRMIQDVLQQETKLGKGDE
                     IGALQRERKKRMEAAEAFADAGRYEQAEDERFEAELITAYLPAQLSDDELAEMVREAI
                     AETGATEPKQMGQV"
     gene            complement(408..704)
                     /locus_tag="P5138_05395"
     CDS             complement(408..704)
                     /locus_tag="P5138_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60043.1"
                     /translation="MGILFMIGCIAPISFFDWIPAALLAGFLIWRGVRSPRYFFPFVA
                     GIGITLFGLGIVNGTYRIMLLYGTVVIAGGIVFYSAFGPRTSHADPPSGGGDEE"
     gene            complement(767..1582)
                     /gene="murI"
                     /locus_tag="P5138_05400"
     CDS             complement(767..1582)
                     /gene="murI"
                     /locus_tag="P5138_05400"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRV60044.1"
                     /translation="MTRSSLPIGVFDSGVGGLTVLHECLISLPAEDFIYLGDSGHFPY
                     GTKTAEELRDRASRIAAEMLGRGVKMLVIACNSATAAAGDEIRSLGSDAGVPVVTVVG
                     PEAEIAASISANGRVGVLATPATVESGAYHHALEAIGKPLTVTEVAAPDLAPIIQEGP
                     TFDEEVLDTVRGYCEPLRQADIDTLILGCTHYPLVAPMLQRMLGRDVSLVTAGHAIAA
                     AIQRELVGRGELSEDRVEGDYRFLVTGDVDSFVEVGTRFLQLPLAGRAERIDV"
     gene            complement(1579..2379)
                     /locus_tag="P5138_05405"
     CDS             complement(1579..2379)
                     /locus_tag="P5138_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRV60045.1"
                     /translation="MLAELTVLAPGGVEEIQGDGYVEYAIYGAEGELPDLGEEEAVIA
                     GGTVEVEATEVADDWADRWRTFHKPLLVGDPESGPAVWIRPPWEPERSDLAEVVVDPG
                     QAFGTGAHPTTRLCVEALIRIAADGGPFGALTDLGCGSGVLAIAAGKLGWGPLEGCDN
                     EVAAVEAARENAEVNAVQASFERINLKAGLPPLAPTTVANLTAPLLEAVAAGLTPGNR
                     PSTLICSGLLTTEYERVLAAFGPHGLVETGRGGMGDWGSLVFECEGAA"
     gene            complement(2421..>2701)
                     /locus_tag="P5138_05410"
     CDS             complement(2421..>2701)
                     /locus_tag="P5138_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRV60046.1"
                     /translation="TIGVTTLDGEDEIELAAGTQPGAEARLKGKGLPRLGSSRRRGDH
                     RFIFNVVVPANLSDEQKKLATDLDETITDENREPKESGIFSRVRRAFG"
BASE COUNT          437 a          918 c          879 g          467 t
ORIGIN      
        1 atgacctggc ccatctgctt cggttcggtg gcgccggtct cggcgatcgc ctccctgacc
       61 atttcggcca gttcgtcgtc gctcagctgg gccggaaggt aggccgtgat cagttcggcc
      121 tcgaaacgtt cgtcttcggc ctgctcgtat cggccggcgt cggcgaatgc ctcggcggct
      181 tccattcgct tcttgcgctc gcgctgcaga gccccgatct cgtcgccctt tccgagcttc
      241 gtttcctgct ggaggacgtc ctggatcatg cgcaatgccg aagcacgctt gcggtcgccg
      301 tccttgagag cggcctgcgc gtctacacgt atctggtcga ctgctgacat ggcactaggg
      361 tacctgcccc gcccgccgaa cccgctcgcc accggtgaag atcgcggtca ctcttcgtcc
      421 ccgccgccgg acggcgggtc ggcgtgtgaa gtccgggggc cgaaggcgct gtagaagacg
      481 atcccgccgg cgatcaccac ggttccgtac aggagcatga tccggtaggt cccgttgacg
      541 atgccgaggc cgaagagggt gatcccgatg cctgccacga aggggaagaa ataccggggc
      601 gagcggaccc cccgccagat caggaagccg gccagcaggg ccgccggtat ccagtcgaag
      661 aagctgatcg gggcaataca gccgatcatg aaaagaattc ccacggccat ccatccgacg
      721 aacttcagag tggcgatatt gcctgtgttg tgctggtcgt ccggcatcac acatcaatcc
      781 tttccgcccg tccggcaagc ggaagctgca ggaaacgggt cccgacctcg acgaaggaat
      841 cgacgtcacc ggtcaccagg aaacggtagt ccccctcgac ccggtcctcg gagagttcgc
      901 cccggccgac cagttcccgc tggatcgcgg cggcgatcgc gtggcccgcg gtcaccaggc
      961 tcacgtcgcg ccccagcatg cgctggagca tcggcgcgac cagcgggtaa tgggtgcagc
     1021 cgaggatcaa cgtgtcgata tcggcctggc gcaggggttc gcagtagccg cgaaccgtat
     1081 cgagcacctc ctcgtcaaag gtgggccctt cctggatgat cggcgcaaga tcgggcgcgg
     1141 ccacctcggt cacggtcagt ggcttgccga tcgcttcgag ggcatggtgg taggcaccgc
     1201 tctcaaccgt ggcgggagtg gccagaaccc cgaccctgcc gttggcactg atcgaggcgg
     1261 cgatctcggc ctccgggccg acgacggtca caacgggtac cccggcatcg gatccgagcg
     1321 agcggatctc atcgcccgcg gcagccgtgg ccgaattgca ggcaatcacc agcatcttca
     1381 cgccccgtcc gagcatttcg gccgcgatcc gggaggccct gtcgcgaagc tcttcggcgg
     1441 tcttggtgcc gtatgggaag tggccgctat cgccgaggta gatgaagtcc tcggccggca
     1501 atgagatcag gcattcgtgc aacacggtga ggccgccgac gccggagtcg aatacaccga
     1561 tcggaagcga ggaacgggtc atgccgcccc ttcgcattcg aaaacgaggg aaccccagtc
     1621 gcccatccca ccacggcccg tctcgaccag cccgtgcgga ccgaatgccg ccaggacccg
     1681 ttcgtattcg gtggtcagga gaccggaaca gatcagggtg ctgggccggt tcccgggcgt
     1741 gaggccggcg gcaaccgcct ccaggagtgg cgcggtcaga ttggcaacgg tggtgggagc
     1801 aagcggaggc aatcccgctt tcaggttgat ccgctcgaag ctcgcctgaa cagcgttcac
     1861 ctcggcgttt tcgcgggccg cctcgacagc cgccacttcg ttgtcgcagc cttccagcgg
     1921 gccccagccg agcttcccgg cagcgatcgc gagaacgccg gacccgcagc caaggtccgt
     1981 cagcgccccg aacggcccgc cgtccgcggc gatccggatc agtgcctcga cgcagagccg
     2041 ggtggtggga tgggcgccgg ttccgaacgc ctgtccgggg tcgaccacga cttcggccag
     2101 gtccgaccgt tcgggctccc agggcggccg gatccagacc gccgggccgg attccgggtc
     2161 accgaccagg agcggcttgt gaaaggtgcg ccagcggtcg gcccagtcgt cggcaacctc
     2221 ggtcgcttcg acttcgaccg tgcctccggc gatcaccgcc tcttcctcgc cgaggtccgg
     2281 caattctcct tcggccccgt agatcgcgta ttcgacatat ccgtcgccct ggatttcctc
     2341 gacacctccg ggggcgagga cggtgagttc ggccaacacc agttcggcct gttccggagc
     2401 gcaccggacc gccaggcgga tcaaccgaat gccctgcgta cccgagagaa gatccccgac
     2461 tcctttggct cgcggttctc gtcggtgatg gtctcgtcga ggtccgtggc cagcttcttc
     2521 tgctcgtcac tgaggtttgc cggaaccacc acgttgaaga tgaagcgatg gtcgccccgc
     2581 cgccgggagg acccgaggcg cggcagtccc ttgcccttca gcctggcctc ggccccgggc
     2641 tgggtgccgg ccgccagttc tatttcgtct tcgccgtcca gggtggtgac tccgatcgtg
     2701 t
//
LOCUS       DAOTIP010000240         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44858_length_2693_cov_3.093252, whole genome shotgun sequence.
ACCESSION   DAOTIP010000240 DAOTIP010000000
VERSION     DAOTIP010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44858_length_2693_cov_3.093252"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="P5138_05415"
     CDS             <1..414
                     /locus_tag="P5138_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRV60047.1"
                     /translation="QGLVEDEDTDGHVDNICALIEPGRVLLQTAPEGDPNVPNARENA
                     GRLADAGIEVVELDLLPRIESQGEQVVIPYTNFYIANGALIVPVSGFDPDMDAQALEL
                     LGRLYPDREAVGIDGRNLALGGGGIHCITQQIPAA"
     gene            complement(425..871)
                     /locus_tag="P5138_05420"
     CDS             complement(425..871)
                     /locus_tag="P5138_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRV60048.1"
                     /translation="MGILEASRTVEIDAPMSECYAIIADLPSTPEWQESMISLDVIET
                     NSEGLASLVEIRSDGKVKEVTSRLAFEYEVEVGMTWEQKKGDMKWLIGSWSLEDLGEG
                     KTKATYWLKADTGRMLGLLIKGPVQDKLKEWLTKDAAEGLKARAES"
     gene            956..1948
                     /locus_tag="P5138_05425"
     CDS             956..1948
                     /locus_tag="P5138_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV60049.1"
                     /translation="MTDLSLNGVHHISCITGDAPGNVEFYAGLLGMRLVKKTVNQDDP
                     SVYHLFYGDEKGTPGFDLTFFEYPGTRDGRAGAGMIHRILWRVGSSESLDYWAERLEA
                     GGVETRRSSDEEAGFFGVGPTGEGDTLLFQDPEGLTHEILIYEGPDEPLVPAHVEIPP
                     EHAIQGFHGARAFSGNREITEQVLGLVLGFTDEGEGKWHLAGEAGEDFEARNGFYFLD
                     EAPEERAVPGAGTVHHIAWASPIDQHPDWREKVAATGLYITPVIDRFYFKALYFREPG
                     GVLFELATIGPGFATDEDQEHLGERLSLPPDFESVREQVEPNLRPIPDITQWRP"
     gene            1992..>2693
                     /locus_tag="P5138_05430"
     CDS             1992..>2693
                     /locus_tag="P5138_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV60050.1"
                     /translation="MSRYEPREIEGKWQRLWADEGTWEVPNPGQEGFDPEKPKSYVLE
                     MLPYPSGEPHVGHLKCYSLGDAIAHFRRRNGFEVIHPMGFDSFGLPAENNAIKTGEPP
                     LVATERSIESFRRQFADWGVSIDWSRELATHTPEYYRWTQWIFLQLFERGLAYRTQAP
                     VQWCPRDATVLANEQVVDGRCERCGTEVETRNLRQWFFKTTEYAQQLLDDFDELESWP
                     PHVITMQRNWIGRSEG"
BASE COUNT          504 a          891 c          838 g          460 t
ORIGIN      
        1 caggggctgg tcgaggacga ggacaccgac ggacacgtcg acaacatctg tgccctcatc
       61 gagccgggcc gggtactgct gcagactgcc cctgaaggtg atccgaacgt tccaaatgcc
      121 cgggagaacg cggggcgcct ggcggatgcc ggcatcgagg tggtcgaact cgacctgctg
      181 ccgcgcatcg aaagccaggg ggaacaggtc gtcattccct acaccaactt ctacatcgcc
      241 aacggggccc tgatcgtgcc cgtcagcggc ttcgatcccg acatggacgc ccaggcgctt
      301 gaactgctgg ggcggctcta cccggaccgg gaagcggtcg gcatcgacgg gcgtaacctc
      361 gccctgggcg gcggcggcat ccactgcatt acccagcaga tccccgccgc ctgaccggca
      421 gggactagct ctcggcgcga gctttcaggc cctcggccgc gtccttggtc agccattcct
      481 tcagcttgtc ctggacgggc cccttgatca gcagcccgag cattcttccg gtatcggcct
      541 tcagccagta ggtggccttc gtcttcccct cgccgaggtc ttcgagcgac cagctgccga
      601 tcaaccactt catgtcgccc ttcttctgct cccaggtcat gcccacctcg acctcgtact
      661 cgaaagcgag ccgggaggtg acttccttga ccttgccatc ggacctgatt tcgaccaggg
      721 acgcgagccc ttcggaattc gtctcgatca cgtcgagcga aatcatcgac tcctgccatt
      781 ccggcgtgga agggaggtcg gcgatgatcg cgtaacactc ggacatgggt gcgtctatct
      841 ctactgtgcg tgaggcttca agaattccca tggccagatg ctatcccggt ttggtggaca
      901 ccaaacgcag ggcgggccct gggtgtcgga ttgcccgccc gggtatcttg gctccatgac
      961 cgatctcagt ctcaacggcg tccatcacat cagctgcata accggcgacg ctcccggaaa
     1021 cgtcgagttc tacgccggcc tgctcggcat gaggctggtc aagaaaaccg tcaaccagga
     1081 cgacccctcc gtctaccacc tgttctacgg agacgaaaag ggaaccccgg gattcgacct
     1141 gaccttcttc gagtacccgg gcacccgcga cggccgggca ggtgccggga tgatccaccg
     1201 catcctctgg cgggtcgggt catcggagtc gctggactac tgggccgaac gactcgaggc
     1261 cgggggcgtc gagacgagga gaagctccga cgaggaagcc ggcttcttcg gggtcgggcc
     1321 gaccggcgaa ggtgacacgc tcctgttcca ggatccggag ggcctgactc acgagatcct
     1381 catctacgaa ggacccgacg agccgctcgt ccccgcccac gtcgagattc cgcccgagca
     1441 cgcgatccag ggcttccacg gtgcccgggc cttctcggga aaccgggaga taaccgagca
     1501 ggtgctcggc ctggtcctcg gattcaccga tgaaggtgaa ggcaagtggc atctggccgg
     1561 ggaggccgga gaagatttcg aggcgagaaa cggcttctat tttctcgacg aggcccccga
     1621 ggagcgggcg gtgcccggcg ccggaaccgt ccatcacatc gcctgggcat caccgatcga
     1681 ccagcatccg gactggcggg aaaaggtggc ggcaaccggg ctctacatca ctccggtgat
     1741 cgaccgcttc tacttcaagg cgctctactt ccgcgagccg ggcggagtcc tcttcgaact
     1801 cgccacgatc ggccccggct tcgccaccga cgaggatcag gaacacctcg gcgaaaggct
     1861 ctcgctaccg ccggatttcg aatcggtccg ggagcaggtc gaaccgaatc tgaggccgat
     1921 ccccgacatc acccagtggc ggccctgagc ggcaggaccg ccccgttgcc ggcgaaatag
     1981 actccgcgcc gatgagtcgc tacgaacccc gggaaatcga gggcaaatgg cagcgcctat
     2041 gggccgacga gggcacctgg gaggtgccga accctggtca ggagggcttc gaccccgaaa
     2101 agcccaagag ctacgtgctc gagatgctgc cctacccttc cggagagcct catgtaggac
     2161 acctgaagtg ctactcgctg ggtgatgcga tcgcccattt ccggcggcgc aacggcttcg
     2221 aggtgatcca cccgatgggt ttcgattcgt tcgggctgcc ggccgagaac aatgcgatca
     2281 agaccggcga gccacccctg gttgcgaccg aacgatcgat cgagtccttc cgtcgccagt
     2341 tcgccgactg gggtgtttcg atcgactggt cgcgggaact cgcgacccac actcccgagt
     2401 actaccgctg gacccagtgg attttcctgc agctcttcga gcgcgggctc gcataccgca
     2461 cccaggcacc cgtccagtgg tgccccaggg atgcaacggt gctggccaac gagcaggtgg
     2521 tcgacggacg ctgcgagcgc tgcggcacag aagtcgaaac ccggaacctg aggcagtggt
     2581 tcttcaagac caccgagtac gcgcagcaac tgctcgacga cttcgacgag ctggagtcct
     2641 ggccgcccca tgtgatcacc atgcagcgga actggatcgg ccgctcggaa ggc
//
LOCUS       DAOTIP010000241         2691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44895_length_2691_cov_2.487102, whole genome shotgun sequence.
ACCESSION   DAOTIP010000241 DAOTIP010000000
VERSION     DAOTIP010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44895_length_2691_cov_2.487102"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="P5138_05435"
     CDS             complement(<1..274)
                     /locus_tag="P5138_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV60051.1"
                     /translation="MSHEIEKPRTYRKRRRADKEAETRLRITEAAVKLHGTIGPAKTT
                     VSGIAREAGVQRATVYRHFPDEAAIYQACTSHYNAQHPPPDLEEWSG"
     gene            356..904
                     /locus_tag="P5138_05440"
     CDS             356..904
                     /locus_tag="P5138_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV60052.1"
                     /translation="MAHAVDTKRIRFDASQGPRLIGPGDGKSVDLGSVGVRFMAWTEE
                     TGGGFSLVEHPIPPKTLVAPVHIHEREDEYSYVLEGRMGAKLGDDVVYAEAGDFVFKP
                     REQWHTFWNPDDAPCRILEIIAPGGFEHFFDELATAMAGAFDPEKLAELGARYGHRFK
                     PETVSELCAEHGLWHPMLEEQG"
     gene            complement(993..1202)
                     /locus_tag="P5138_05445"
     CDS             complement(993..1202)
                     /locus_tag="P5138_05445"
                     /inference="COORDINATES: protein motif:HMM:NF039788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HRV60053.1"
                     /translation="MTFGFSLFLVAVGAILAFAVTATVSGVDINIVGWILMGVGAFGL
                     IFSIFAYGRRGRLPGDPPRDLPPRY"
     gene            1343..2008
                     /locus_tag="P5138_05450"
     CDS             1343..2008
                     /locus_tag="P5138_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HRV60054.1"
                     /translation="MAWISLGVAFLCAIIILWDIFVAGNRQPMGVMEAVWPITALYLG
                     PLGLWAYWHWARVGRPEEQDQGEPRPKPFPVKVAIGVSHCGAGCTLGDIIATGIVFAL
                     GLTIAGLALPYEYVFDFTFAFLLGIVFQFFAIRPMRPEMPLGQVLKKALKADTLSLTA
                     FEVGLFGWMAIMQLVLFKNPHLSPEHASYWFLMQIGMAIGFMTSYPVNIWLIRRGIKE
                     TMA"
     gene            2251..>2691
                     /locus_tag="P5138_05455"
     CDS             2251..>2691
                     /locus_tag="P5138_05455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60055.1"
                     /translation="MVIGAGIHGISSALALASRGRRSLVLEAGTGPFFGASARNEGKI
                     HLGFVYALDETGATTKAMVEGSLSFAPLIEHWCGPLPWEQIRSDGFGYVVMDEGLAGP
                     DQVEAHYRSVIEEAERAAPAFGSNYVGVNLGGAEIVRHEGTFPGM"
BASE COUNT          485 a          815 c          879 g          512 t
ORIGIN      
        1 cgccgctcca ctcttcgagg tcggggggtg gatgctgggc gttgtagtgg gaggtgcagg
       61 cctggtagat cgccgcttcg tcgggaaagt gccgatagac ggttgcccgt tgcacaccgg
      121 cttcacgggc gatgccactg accgtcgtct ttgccgggcc gatcgtgccg tgaagcttca
      181 ccgcggcctc ggtgatccga aggcgcgtct ccgcctcctt gtcggctcga cgacgcttcc
      241 ggtaggtgcg gggcttttcg atttcgtgag acatacctgt tcatgcaata ttgacagatg
      301 ggcaggacgt gtgttacttt cgcaaaacag tcctgttcaa tgaaaagtag aaggtatggc
      361 acacgcggta gacaccaaga gaattcgctt cgacgcaagc cagggtccga ggctgatcgg
      421 gccgggtgac ggaaagtcgg ttgacctcgg ttcagtcggg gtgcgcttca tggcctggac
      481 cgaagaaacc ggcggaggct tctcgctggt cgagcatccg atccccccga agaccctggt
      541 tgctccggtt cacatccacg agcgcgagga cgaatacagc tacgtgctgg aaggccgcat
      601 gggcgcgaaa ctcggcgacg acgtcgtcta tgccgaggcc ggggatttcg tcttcaagcc
      661 gcgcgagcag tggcacacct tctggaatcc cgacgacgcg ccctgccgca tcctcgagat
      721 catcgctccg gggggcttcg agcacttctt cgacgagctg gccacggcaa tggccggcgc
      781 cttcgacccg gagaagctgg ctgaactcgg cgccaggtac gggcaccgct tcaagccgga
      841 gaccgtgtcc gagctctgcg cggagcacgg cctgtggcat ccgatgctgg aagagcaggg
      901 ctagccgacc ggatgacgag agtgccaaat gcggccgggc cgcctgacca ggacggcagg
      961 tgccgagacg attacgcctc tgtctctgag agctagtacc tcggaggcag atctcgaggt
     1021 ggatcgccag gcaggcggcc ccgtcgacca taggcaaaga tcgagaagat caggccgaac
     1081 gcaccgacgc ccatcaggat ccagccgacg atgttgatgt cgacgccact gacggtcgcg
     1141 gtgaccgcga aggcgaggat tgcccccacc gcgacaagaa agagtgaaaa accgaaagtc
     1201 atggtgagcc ggtacccggc ggcatttccg ggcaaacagg ccaagcgccg cttcgccgtc
     1261 gttgacggtc gccgattctg tttgcccgga gggtggtggg atactggatc cagtaccgat
     1321 gcctccgcaa tggcttgaga ccttggcttg gatatcgctc ggcgtggcgt ttctttgcgc
     1381 catcatcatc ctctgggaca tcttcgtcgc cggaaaccgt cagccgatgg gggtcatgga
     1441 ggcggtgtgg ccgatcacgg ccctctacct ggggccgctc ggcttgtggg cctactggca
     1501 ttgggcccgg gtcgggcggc ccgaggaaca ggatcagggt gagccgcgcc cgaaaccgtt
     1561 cccggtcaag gtggcgatcg gggtcagtca ctgcggcgcc ggatgcaccc tcggcgacat
     1621 catcgccacc ggcatcgtct tcgccctcgg cctcacgatt gccggccttg ctctccccta
     1681 cgaatacgtc ttcgatttca ccttcgcttt cctgctcggg atcgtcttcc agttcttcgc
     1741 gatccggccg atgaggccgg aaatgcctct gggtcaggtg ctgaagaagg ccctcaaggc
     1801 cgacaccctc tcgctgaccg ccttcgaggt cgggcttttc ggctggatgg cgatcatgca
     1861 gctggtcctc ttcaaaaacc cgcacctttc gcctgagcat gcctcgtact ggttcctgat
     1921 gcagatcggg atggcgatcg gcttcatgac cagctacccg gtcaacatct ggctgatccg
     1981 tcgcggaatc aaggagacga tggcctgaat caggtccttc ggggccttgc tgatacggtg
     2041 aaatctccga tgaccggggg cccgggttga gaatcggatg caggatcaac gccgcgatag
     2101 cggcagttat cgccccttcg ggagacgggg cgaaggagtc tcaggggccc gacgcaagcg
     2161 gctgatttgc gtttcggcca gtcccatctt ctcgcttcct cgaatggaat tctggatgtc
     2221 gcgacaatga aaagtgaacg ggaggagacg gtggtcatcg gtgccggcat tcacggcatc
     2281 tcgtcagccc ttgcgctggc ttcccgcggc aggcggtccc tggtgctcga ggccggcacc
     2341 ggccccttct tcggagccag cgcccgcaac gaagggaaga ttcatctggg tttcgtctac
     2401 gccctcgacg agaccggtgc gacgacaaag gcgatggtcg aaggatcgct ctccttcgca
     2461 ccgctgatcg aacactggtg cggtccgttg ccctgggagc agatccgctc cgacggcttc
     2521 ggctacgtgg tgatggacga aggactggcc gggcccgacc aggtggaagc ccactacagg
     2581 tcagtcatcg aggaggcaga aagggcggct cccgccttcg gttcgaacta tgtcggggtg
     2641 aacctcggcg gcgccgaaat cgtccggcat gaagggacct tccccggcat g
//
LOCUS       DAOTIP010000242         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_44999_length_2686_cov_3.878373, whole genome shotgun sequence.
ACCESSION   DAOTIP010000242 DAOTIP010000000
VERSION     DAOTIP010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44999_length_2686_cov_3.878373"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..978)
                     /locus_tag="P5138_05460"
     CDS             complement(73..978)
                     /locus_tag="P5138_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRV60056.1"
                     /translation="MSLTVVGSIAFDAVTTPFGERERMLGGSAVHFSLAASFFTDVRV
                     VGPVGDDFTQTELDVLENRGVVTADIERVSGETFFWKGHYEYDLNTAHTDDTRLNVFG
                     DFEPKLSETSASSDMLFLANIQPDLQRQVRAQCEGAGFVGLDTMNLWIDTAKPSLEKA
                     ISEVDCVLINDAEIRQFTGESNLARAATAVMAMGPRAVIAKQGEYGAALFTPSSFFAL
                     PGFPLEDVRDPTGAGDSFAGGFLGYLDGEAGEIDQQALRTAMGYGTVLASYNVEEFGT
                     ERVGRLTRDEVESRLEALKSMTAFA"
     gene            1017..1964
                     /locus_tag="P5138_05465"
     CDS             1017..1964
                     /locus_tag="P5138_05465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRV60057.1"
                     /translation="MKAIQIEEFGGPEVMNLVELPDPVPAEGEVLIEISRTGVNFSDT
                     HITNDQYVAKQQLPLVPGIEFVGRTGDGKRVAAVVPNGAYAERIVVPESVLVPIPDEI
                     DDDTAAGILIQGVTADGVLTLSGRLREGETVVVGAAAGGTGSLAVQLARSMGAGTVIG
                     LASGEEKQKLVRDLGADVVIDSRSESLGEEVRVAAEGPVDLVLEMAGGSSFEQLLPTI
                     GPFGRMVVVGIATREQNEVRTGKLLKNSLTVSGFWLMHLLARPELAGESIDRVFRATA
                     RGDLKPVVSGTYGLTDVRQVHEDIASRKTVGKLLLDPTR"
     gene            2012..>2686
                     /locus_tag="P5138_05470"
     CDS             2012..>2686
                     /locus_tag="P5138_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV60058.1"
                     /translation="MPEIQQAIDEIGFEKPSPIQAEAIPELLAGHDVIGQAQTGTGKT
                     AAFGLPMLQYLDPANDEVQAIVLTPTRELCIQVTQALRSYAEHLDIEIVAVFGGAPIR
                     SQQAQLRSGAHVVVATVGRMKDLISRRSLVLTAARFVVLDEADEMLDLGFIEDVERIL
                     RMCPSGRQTALFSATMPPPIKRLADTFMYDPVTISITPKTLTVDAIEQAYVEVPGRAK
                     VDKLIEV"
BASE COUNT          505 a          834 c          885 g          462 t
ORIGIN      
        1 cggtctgctg ttcggtcgac atgcttctcc tcgatgctgg tctctgatct ttgtcaggga
       61 ccagtatttc agttaagcga aagcggtcat cgatttcagc gcttcgagcc tggactcgac
      121 ctcgtcccgg gtcagccggc cgacccgctc ggtgccgaac tcctcgacgt tgtaggaggc
      181 cagcacggtt ccgtagccca ttgcggtcct cagggcctgc tggtcgatct ccccggcctc
      241 gccgtcgagg tatccgagga agccaccggc gaaggagtcg ccggctccgg tcggatcccg
      301 tacgtcctcg agcgggaagc cgggcagggc gaagaaggaa cttggcgtga agagcgcggc
      361 accgtactcg ccctgtttgg cgatcaccgc gcggggtccc atcgccatca cggccgtggc
      421 ggcgcgggca aggttggatt ccccggtgaa ctgcctgatc tcggcgtcgt tgatgagcac
      481 gcaatcgacc tctgaaatcg ccttctcgag cgacggcttc gccgtgtcta tccagaggtt
      541 catggtgtcc aagccgacga aaccggcccc ctcgcactgg gcccggacct ggcgctggag
      601 gtcgggctgg atgttcgcga ggaacagcat gtcggaactg gccgaggttt ccgagagctt
      661 gggctcgaaa tcaccgaaca cgttcagtcg ggtgtcatcg gtatgggcgg tattgaggtc
      721 gtactcgtag tggcccttcc agaagaaggt ctcaccgctc acccgctcga tgtcggcggt
      781 gacgacgcca cggttctcca gcacgtcgag ctcggtctgg gtgaagtcgt cgccgaccgg
      841 tccgaccaca cggacgtcgg tgaagaagct ggcagccagc gagaagtgga ctgcggagcc
      901 gccgagcatg cgctcgcgct cgccgaaagg ggtggtgacc gcgtcaaatg cgatcgaacc
      961 gacaacggtc agagacatgg ccgggatcgt actcaccacc gtgacaagat ggtttggtga
     1021 aagcgatcca gatcgaggag ttcggcggtc ccgaggtgat gaacctcgtc gaactgcccg
     1081 acccggtgcc tgccgagggt gaggtcctga tcgagatcag ccgcaccggg gtcaacttct
     1141 ctgacactca catcaccaac gaccagtacg tggcgaagca gcaactgccc ctcgtgcccg
     1201 gaatcgagtt cgtcggcagg acgggtgacg ggaaacgggt tgccgcggtg gtgccgaatg
     1261 gcgcctacgc cgaacggatc gtcgtgccgg agtcggttct ggtgcccatc ccggatgaga
     1321 tcgacgatga cacggcggcg gggatcctga tccagggtgt gacggcggac ggcgtcctga
     1381 ccttgagcgg ccggctccgg gaaggagaaa ccgtggtcgt cggtgcggcg gccggcggca
     1441 ccggctccct ggcggtccag ctggcgcggt cgatgggggc cggcacggtc atcgggttgg
     1501 catcggggga agagaagcag aagctcgtca gggacctcgg tgccgacgtc gtgatcgact
     1561 cgaggagcga atcgctcggc gaggaagtcc gcgtcgcagc ggagggtccg gtcgacctcg
     1621 tcctggaaat ggcgggaggc agcagcttcg aacagctgct cccgacaatc ggtccgtttg
     1681 gccggatggt cgtggtcgga atcgccaccc gggagcagaa tgaggtcagg actggcaaac
     1741 tgctcaagaa cagcctcaca gtttccggct tctggctcat gcatctgctg gccagaccgg
     1801 agctcgccgg cgaatcgatc gaccgggtct tcagggccac cgccaggggc gatctcaaac
     1861 cggtggtaag tggaacctac ggcctcaccg atgtgaggca ggtccatgag gacatcgcct
     1921 cgcgaaagac agtcggcaag ttgctgctcg acccaaccag atgacagaac agacagaaca
     1981 agaagaaaga cttttcaaag acctcgggct gatgcctgag atccagcagg caatcgacga
     2041 gatcggattc gagaaaccga gcccgatcca ggcggaagcc attcccgagt tgctcgccgg
     2101 ccacgacgtg atcggccagg cgcagaccgg tacaggcaag acggccgcct tcggcctgcc
     2161 gatgctgcag tacctcgatc cggcgaacga cgaggtgcag gcgatagtcc tcaccccgac
     2221 ccgcgagcta tgcatccagg tcacccaggc gctcaggtca tacgccgagc atctcgacat
     2281 cgagatcgtc gccgtcttcg gtggtgcccc gatccgcagc cagcaggccc agctccggtc
     2341 cggggcccac gtcgtagtcg cgacggtggg ccgcatgaag gacctcattt ccaggcgatc
     2401 tctggttctg accgcggctc gcttcgtggt actcgacgaa gccgacgaga tgcttgacct
     2461 cggcttcatc gaggacgtcg agcggatcct ccgcatgtgt cccagcggcc ggcagaccgc
     2521 gcttttctcg gccaccatgc cgccgccgat caaacggctt gccgacacct tcatgtacga
     2581 cccggtgacc atttccatca cccccaagac cctcaccgtg gatgcgatcg agcaggctta
     2641 cgtcgaggtg cccggccggg ccaaggtcga caagctgatc gaagtg
//
LOCUS       DAOTIP010000243         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45184_length_2679_cov_3.647104, whole genome shotgun sequence.
ACCESSION   DAOTIP010000243 DAOTIP010000000
VERSION     DAOTIP010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45184_length_2679_cov_3.647104"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5138_05475"
     CDS             complement(<1..225)
                     /locus_tag="P5138_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRV60059.1"
                     /translation="MIYLETPKKFRPLVTQANHVAREIFRPISRKYDEAEHEYPKELD
                     VLASLIEGMNEGSGMAAGAAGVRGEEDGSRE"
     gene            complement(252..1595)
                     /locus_tag="P5138_05480"
     CDS             complement(252..1595)
                     /locus_tag="P5138_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60060.1"
                     /translation="MSSNNGQGLADKTMGSSLRALNWIAGSTILDRLGIRSQFERTVF
                     QGTKHGMRAATSAGRTFRKASTLGKPARQNSGSASGLFDLTPDDEQQMFIEAGRAFAA
                     DQLRPAAAEADGQKATPQEVLDQASEIGVNTLGVPEEFDGVLTEQSATTWTLMAEALA
                     HGDMGIAYAALAPGAVATAIGRWGDPDQQATYLPAFTGEEPPAAALALLEPRALFDPF
                     TLDTTAIPAADGSWILSGVKSLIARPGDCELFVVAAATGDGQPALFIVESGAEGLGTE
                     PEPAMGLRAASTGRLVLEKVRVPEGAVIGDETDRSRIYADCVAGSRVAWAAMSTGTSQ
                     AVLDYVIPYVNERKAFGEPVSNRQSVAFSVSDIAIETGGMRLANWRAASRSDRGDSFH
                     REAALARRLAAAKGMDIGSAGVQLLGGHGYVTEHPVERWYRDLRAAGVMEGALLA"
     gene            complement(1597..2505)
                     /locus_tag="P5138_05485"
     CDS             complement(1597..2505)
                     /locus_tag="P5138_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRV60061.1"
                     /translation="MSTLDSSPSILPLYVKAALPLLPGASSLPFVPGGGGEIPSGLEL
                     ELKGVRPERDRVADYAKVCGFRLREELPPTFPHILAFPLHMAVMSDGRFPFGAVGLVH
                     IENTITRHRPVTFEDAQDIRVGVSRLKAHPKGQSFSLLTEVGVEGETVWTETSTMLRR
                     GKTFGEEQAEPAPFPTLGAETPASATWRLSGDLGRRYGAVSGDRNPIHMHALTARPLG
                     FDSAIAHGMWTLARSLAQLENRLPDSFTVAARFRRPIHLPGRVGFASGDEGERTLFSV
                     RDAGDGTPHLDGTIDPLPTDTAKASV"
BASE COUNT          401 a          871 c          946 g          461 t
ORIGIN      
        1 ttcgcgggac ccgtcttcct cgccgcggac accggcggca ccagccgcca ttccggagcc
       61 ctcgttcatc ccttcgatca gggaggcaag gacatccagt tccttcgggt actcgtgttc
      121 ggcctcgtcg tacttgcgcg agatcggacg gaatatctcc cgggcgacgt ggtttgcctg
      181 ggtgaccagg ggtcggaact tcttcggtgt ttcgagatag atcatctgtt cctttcgctt
      241 ggcctgagag ctcaggcgag cagggcgccc tccatcacgc ccgctgcgcg gaggtcgcga
      301 taccagcgct cgaccgggtg ctccgtgacg tatccgtggc cgccgagcag ctggactccg
      361 gccgaaccga tgtccatgcc cttcgctgcg gccagcctgc gggccagcgc ggcctcgcgg
      421 tggaaggaat cgcctcggtc ggaccggctc gcggcccgcc agtttgcgag tcgcatcccg
      481 ccggtctcga tcgcaatgtc ggaaacgctg aaggcgacgc tctggcggtt tgagaccggt
      541 tccccgaagg cctttctctc gttcacgtag gggatcacgt agtcgagcac ggcttgtgac
      601 gttccggtcg acatcgcagc ccaggcgacg cgggatccgg cgacgcaatc cgcatagatg
      661 cgggagcggt cggtttcatc gccgatcacc gcgccctcgg ggaccctcac cttttcgagg
      721 accagacggc cggtggaggc tgcccgcaaa cccatcgccg gctccggctc ggtgccgagt
      781 ccctcggcac ccgactcgac aatgaacagg gcaggctggc cgtcgccggt ggctgcagcg
      841 acgacgaaga gttcgcagtc gcccggcctg gcgatgagcg atttgactcc gctcagaatc
      901 cagcttccgt ccgcagcggg gattgcggtc gtgtcgaggg tgaacgggtc aaagagtgcc
      961 ctgggctcca gcagggccag ggccgcggcg ggcggctcct cgccggtgaa ggccggcagg
     1021 taggtcgcct gctggtcggg gtcaccccag cgtccgatcg cggtcgccac cgctcccggg
     1081 gccagggcag cgtaggcgat gcccatgtcc ccgtgggcga gcgcttcggc catcagggtc
     1141 caggtcgttg ccgactgctc ggtgagcacg ccgtcgaatt cctccgggac tccgagcgtg
     1201 ttgacgccga tctcgctggc ctggtcgagc acctcctgcg gcgtcgcctt ctgcccgtcg
     1261 gcttcagcgg cggccggccg gagctggtcg gcggcgaatg cgcgacccgc ctcgatgaac
     1321 atctgctgct catcgtccgg ggtcaggtcg aagagaccgg atgccgaacc gctgttctgc
     1381 cgggcgggct tgccgagagt ggaggccttc ctgaacgtgc ggccagcgct ggtggcggca
     1441 cgcatgccgt gcttggtgcc ctggaagacg gttcgctcga actggctgcg gattcccaga
     1501 cggtcgagga tcgtcgatcc ggcaatccag ttcagggccc ggaggctgct gcccatggtc
     1561 ttgtcggcca gtccctggcc gttgttgctg ctcatttcaa acgctcgctt tcgcggtgtc
     1621 ggtcgggagg gggtcgatgg ttccgtcgag gtgaggggtt ccgtcgcccg cgtcgcggac
     1681 ggagaagagg gttcgctcgc cttcgtcacc cgatgcgaac cccacccggc cgggcaggtg
     1741 gatcggccgg cggaagcgtg ccgcgacggt gaaggagtcc ggaagacggt tttccagctg
     1801 ggcaagggaa cgggcgagcg tccacatgcc gtgcgcgatg gccgaatcga aaccgagggg
     1861 gcgggcggtc agcgcatgca tgtggatcgg gttgcggtcg ccggagaccg ctccataccg
     1921 gcgtccgaga tcgcccgaga gcctccaggt ggcactggcc ggggtttccg cccccagcgt
     1981 gggaaagggt gcgggttcgg cctgttcctc gccgaatgtc tttccccggc ggagcatggt
     2041 gctggtttcg gtccagacgg tctcgccctc gacaccgacc tcggtgagca gggagaagct
     2101 ctgtcccttc ggatgggcct tcagccggct caccccgacc cggatgtcct gggcatcttc
     2161 gaaggtgacc ggccgatgcc gggtgatcgt gttctcgatg tgaaccaggc cgactgcccc
     2221 gaaggggaac ctgccgtcac tcatgactgc catgtgaagc gggaaggcca ggatgtgggg
     2281 gaaggtgggc ggcagctctt cccggagacg gaaaccgcag accttcgcat agtcggcgac
     2341 ccggtcccgc tccggccgga cacccttgag ttcgagttcg agacccgacg ggatttcgcc
     2401 gcccccgcca ggcacgaaag gcagcgacga cgcacccggc aggagcggca gggcagcctt
     2461 cacgtaaagg ggaaggatcg agggggagga gtcgagtgtg ctcatcaggc accaagcagg
     2521 ttctgtccgc agacccggac cacgtttccg ttgacgccgg tcgaggccgg gctggcgaac
     2581 caggcgatcg tctcggcgac gtccaccggc ctgccaccct gggacaggct gttgaggcga
     2641 cggccgacct cgcgcgggcc gaaaggcatc gcgcgggcc
//
LOCUS       DAOTIP010000244         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45207_length_2678_cov_3.368281, whole genome shotgun sequence.
ACCESSION   DAOTIP010000244 DAOTIP010000000
VERSION     DAOTIP010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45207_length_2678_cov_3.368281"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="P5138_05490"
     CDS             <1..271
                     /locus_tag="P5138_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV60062.1"
                     /translation="MDTPDGKGRVELVEFHSPEAIEGNAGASANALGLRHLAIEVDDL
                     KETLERLKPHGAELIGQIENYQDTYLLCYVRGPEGAIFELIESIG"
     gene            complement(278..1483)
                     /locus_tag="P5138_05495"
     CDS             complement(278..1483)
                     /locus_tag="P5138_05495"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV60063.1"
                     /translation="MSWPKPSERIAELIRLCVNRLLEDPDELFQSINEASIPPEVAGS
                     GSDPVLLRTYRRVNQATLLQWAEANLREPGCEVPPYSGSEITALTRDLVRRGLDTNAL
                     EPYRAGQNITWQKWMEAAFEETDDLDELRELLEISARSIFAYVDATMMVSAERIQQQR
                     EEFNQTGAERLEMVVLVLEENPIDLEYAASRLNYGFDGTHLAAVIWSERNDDPEGLEA
                     AAERLKRAAGAGNALTVHATSAALWAWVKPTRELAEIEAELEDGGEFRFALGTPLQGV
                     EGFRQSHADASEVQRLVLRLGTDLEIARYDAMKVVALLTHDERRAARFVDETLGELAG
                     APMNLKDTVRTYLRNQSNATRTAEALFAHRNTILARIAKADEFLPLPLAESSFEVSAA
                     LEILHWQED"
     gene            1664..>2678
                     /locus_tag="P5138_05500"
     CDS             1664..>2678
                     /locus_tag="P5138_05500"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV60064.1"
                     /translation="MDLEASRHDELDVLIIGAGVSGIGAAYHLQKEQPGKSFAILEAR
                     NDLGGTWDLFRYPGIRSDSDLYTFGYEFKPWTDDKAIADGPAIKAYVKETAEENGIDR
                     KIRYGHKVQRAEWSSEDARWTLTVELADTGETFEMKAKWIFAATGYYRYDEGYMPHFE
                     GIADYEGQIIHPQHWPEDLDVSGKRVVIIGSGATAVTLLPELAKEAEHVTMLQRSPTY
                     ILPVPEKDRVALLLRRVLGETRAYRIIRRKHILRQKAFYQFCQRFPKTARAFIRRVNT
                     KELEGSDCDVDVHFNPTYDPWDQRLCAVPDGDLYEALKNGSSSVVTDRIETFTPNGLK
                     LESG"
BASE COUNT          522 a          848 c          829 g          479 t
ORIGIN      
        1 gatggacacg ccggacggca aggggagggt ggagctggtc gaattccact cacccgaagc
       61 gattgaaggc aacgcaggcg cctcggccaa cgccctcggt ctccggcatc tggcgataga
      121 agtcgacgat ttgaaggaga ccctcgaacg gctgaaaccc cacggcgccg agctgatcgg
      181 ccagatcgag aactaccagg acacctacct gctctgttat gtgcgcggac ccgagggagc
      241 gatcttcgag ctgatcgagt cgatcggctg acccgtttca gtcttcctgc caatgaagga
      301 tttcgagggc tgccgaaact tcgaatgatg actctgcgag aggcaggggc aagaactcgt
      361 cggccttggc gatccgggcc aggatcgtgt tgcggtgggc gaacagcgct tcggccgtcc
      421 tggtcgcgtt cgactggttc ctgagatagg tgcgaaccgt atccttcagg ttcatcgggg
      481 caccagcgag ttcgccgagg gtttcgtcga cgaagcgcgc cgcccgccgt tcatcgtggg
      541 tgagcagggc cacgaccttc atcgcgtcgt agcgggcgat ttcgagatcg gtgccgagcc
      601 ggaggaccag acgctgaacc tccgaggcgt cggcgtggct ttgcctgaaa ccctcaacgc
      661 cctgcagggg cgttccgagc gcgaatctga attcgccgcc atcctcgagt tccgcctcga
      721 tttcggcgag ttcgcgggtc ggcttgaccc aagcccagag cgcggcgctg gtcgcatgga
      781 cggtcagggc attacccgct ccggcggcac gcttcagcct ttccgccgct gcctccagcc
      841 cctcagggtc atcgtttcgc tcgctccaga tcacggcggc gagatgggtc ccgtcgaagc
      901 cgtagttgag gcgggatgcc gcgtactcga ggtctatcgg attctcctcg agaaccagca
      961 ccaccatttc gagccgctcg gcgccggtct ggttgaactc ctcccgctgc tgctggatcc
     1021 tctccgcgct caccatcatg gtcgcatcga cgtaggcgaa gatcgaccgg gccgagattt
     1081 cgagcagttc gcgcagctcg tccaggtcat ccgtctcttc gaaggctgct tccatccact
     1141 tctgccaggt gatgttctgg ccggcccgat agggctccag agcgttggtg tcgagtcccc
     1201 gacgaaccag atcccgggtt agcgccgtga tctcggaacc ggagtagggg ggaacctcgc
     1261 atccgggttc ccgcaggttc gcctcggccc attggagtag cgtcgcctga ttcacccgcc
     1321 ggtaggtacg caggagaacc gggtcactgc cagacccggc tacctcgggg ggaatgctcg
     1381 cctcgttgat cgactggaag agttcgtcgg ggtcttcgag caggcggttc acgcaaaggc
     1441 ggatcagttc ggcgatgcgt tcggagggct tcggccagga catgcgtgaa ggctacccat
     1501 gagtgggcga aaaacacgaa agacgaacgg tcaggtgcgc aatttgccac agcgatgtgc
     1561 atttcgcaca aacagaggcg aaagtctgga acttccaacc ttgagttagt gcaggcgcac
     1621 gaggaacatt tccgtatccg gcgatctacc cgaaggaccc gaaatggatc tcgaagccag
     1681 caggcatgat gaactcgacg ttctgatcat cggtgccggc gtatccggca tcggcgccgc
     1741 ctaccacctt cagaaggaac agcccggcaa gagcttcgcc attctcgagg cccgcaatga
     1801 cctcggtggc acctgggatc tcttccgcta ccccgggatc cgttcggact ccgaccttta
     1861 caccttcggc tacgagttca agccctggac cgatgacaag gcgatcgcgg acggaccggc
     1921 gatcaaggcc tatgtcaagg aaaccgccga ggagaacggg atcgaccgga agatccgata
     1981 tggccacaag gttcagcgag ccgagtggtc atccgaggat gcccgctgga ccctgacggt
     2041 cgaactcgcg gacacggggg agaccttcga gatgaaggcg aagtggatct tcgccgcaac
     2101 cggctactac cgatacgacg agggttacat gccgcatttc gaggggatcg ccgactacga
     2161 agggcagatc atccatcccc agcactggcc ggaggacctc gacgtgtccg gcaagcgggt
     2221 cgtgatcatc ggcagcggag caaccgcggt caccctgctg ccggaactcg ccaaagaggc
     2281 cgagcacgtg accatgctgc aacgctcgcc cacctacatc ctgccggtcc ccgagaaaga
     2341 ccgggtcgcc ctgttgctcc gccgcgttct tggcgagacc cgggcctacc ggatcatccg
     2401 ccgcaaacac atcctccggc agaaggcctt ctaccagttc tgccagcgct tcccgaagac
     2461 cgccagggcc ttcatccggc gggtcaacac gaaggagctc gagggctcgg actgcgatgt
     2521 cgatgtccac ttcaatccca cctacgaccc ctgggaccag cgcctttgcg cggtaccgga
     2581 cggcgacctc tacgaggcgc tcaagaacgg ctcttcctcg gtggtcaccg accggatcga
     2641 aaccttcact ccgaacggac tgaagctcga atcaggca
//
LOCUS       DAOTIP010000245         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45305_length_2674_cov_3.281787, whole genome shotgun sequence.
ACCESSION   DAOTIP010000245 DAOTIP010000000
VERSION     DAOTIP010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45305_length_2674_cov_3.281787"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="P5138_05505"
     CDS             <1..747
                     /locus_tag="P5138_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRV60065.1"
                     /translation="IDKYDPDIFCMQETKAEQGQAEIDLPHYEEYWNSAAKKGYSGTA
                     IFTRQKPIAVLNDFAAEISDEYSLHNDSYGDPRAEGRVITAEYDDFYLVTTYVPNAKR
                     DLSRLKLREKQWDPALLHHMKMLDAKKPVLVCGDMNVAHTEDDLANPKANVGEHGFTD
                     EERQGFQNFLDAGFVDTFRLFTEGNGNYSWWSYMSKARERNAGWRIDYFLASERLEKA
                     ITGAEIHTDVMGSDHCPVSVELDEKKLAKI"
     gene            complement(835..1950)
                     /locus_tag="P5138_05510"
     CDS             complement(835..1950)
                     /locus_tag="P5138_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60066.1"
                     /translation="MNPLKKYIALVIAMVVPFALVANANAADETVTATTDLSPKSGTF
                     YKEAQVASELKLRAEVTTPNTSPKVNPMKNVKVTFPAGMTFKPNNSKTPVCPDSKLSP
                     SSNLSDPSGVVSACSKSVVGTGTAAIYLAKINQPTALIGDPILVVFNAGTNNSNQPKL
                     KIYSYSKTTNVGILMTGTLKGSVLDIAVPVLSNDSAVKYFELDLPGDQLDRPDIGVNT
                     HGLDPNYVQAKCASSPLKTNAVFELGERAYPSGTETGPTTTVTSPQTTQDCVGNAGKV
                     KLGGMKVKGPSAVKNGKKGTYKVTVKNTGTRTAKNVVVTNNRGGKAKDGNIAPGKTKT
                     VKVKAKIKGKKGKKVAEKFTAKSGNVKAKATKKVRVK"
     gene            complement(2143..>2674)
                     /locus_tag="P5138_05515"
     CDS             complement(2143..>2674)
                     /locus_tag="P5138_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60067.1"
                     /translation="DIPGSGIQVETDNGTETVKGLDPNYARSKCSSGKWTTDGTFTLG
                     ERAFPGGTDTGPETIVKATPFTMNCKGLKGNPRLKKARAYGPKRLRRGSRKVFKVKVK
                     NTGTAVARGVKVKAYGSARGSSRMANIWPGQTRWTKVKARVTGRKGSKAKIVFRINPK
                     NGRATKTVAWGRILRR"
BASE COUNT          440 a          803 c          862 g          569 t
ORIGIN      
        1 attgacaagt acgaccctga catcttctgc atgcaggaga ccaaggcgga gcagggccag
       61 gccgagatcg acctccccca ttacgaggag tactggaact cggccgccaa gaagggctac
      121 tcgggcaccg cgatcttcac ccgccagaaa ccgatcgccg tgctcaacga tttcgccgcc
      181 gagatctccg acgagtacag ccttcacaac gattcctacg gtgatccgcg tgccgaaggc
      241 cgggtcatca ccgccgagta cgacgacttc tacctggtca cgacctacgt tcccaacgca
      301 aagcgcgacc tctcgcgcct gaagctgcgg gagaaacagt gggaccccgc cctccttcac
      361 cacatgaaga tgctggacgc gaagaagccg gttctggtct gcggggacat gaacgtcgcc
      421 cacaccgagg atgacctggc caacccgaag gcaaacgtgg gtgagcacgg cttcaccgac
      481 gaggaacgcc agggcttcca gaacttcctc gatgcgggct tcgttgacac cttccgcctc
      541 ttcaccgaag gaaacggcaa ctactcgtgg tggtcgtaca tgtcaaaggc ccgggagcgg
      601 aatgcgggct ggcggatcga ctatttcctc gcatccgaac gcctggagaa ggcgatcacc
      661 ggtgccgaga tccacaccga cgtgatgggc tccgaccact gtccggtcag cgtcgagctg
      721 gacgagaaga agctggcgaa gatctgagcg gttccggctg cgcccggaac cctgttgatg
      781 cacgaaaaag ggcggcccgg aggccgccct tcttcgctgt tgattgaggc agtcttactt
      841 gacgcggacc ttcttggtgg ccttcgcctt gacgttgccg gacttggcgg tgaacttctc
      901 ggcgaccttc ttgcccttct tgcccttgat cttggccttg accttgacgg tcttggtctt
      961 gccgggggcg atgttgccgt ccttggcctt gccgccgcgg ttgttggtca caacaacgtt
     1021 cttggcggtg cgggtgccgg tgttcttgac ggtgaccttg taggtgccct tcttgccgtt
     1081 cttgacggcg ctggggccct tgaccttcat gccaccgagc ttgaccttgc cggcgttgcc
     1141 gacgcagtcc tgggtggtct ggggcgaagt gaccgtggtg gtcgggccgg tctcggtgcc
     1201 gctcgggtag gcgcgctcac cgagctcgaa gacggcgttg gtcttcagcg ggctgctggc
     1261 gcacttggcc tggacgtagt tcgggtcgag gccatgggtg ttgacgccga tgtcggggcg
     1321 gtccagctgg tcacccggca ggtcgagctc gaagtacttg acagccgagt cgttggacag
     1381 gaccggaacg gcgatgtcga ggacgctgcc cttgagggtg ccggtcatca ggatgccgac
     1441 gttggtggtc ttcgagtagc tgtagatctt cagcttcggc tggttgctgt tgttggtgcc
     1501 ggcgttgaag acgaccagga tcggatcacc gatcagcgcg gtcggctggt tgatcttggc
     1561 caggtagatg gccgcggtac cggtaccgac aaccgacttc gagcacgcgg aaaccacgcc
     1621 cgaggggtcg gacaggttcg agctcgggct gagcttgctg tccgggcaga ccggggtctt
     1681 cgagttgttc ggcttgaagg tcatgccggc cgggaaggtc accttgacgt tcttcatcgg
     1741 gttgaccttc ggcgaggtgt tcggcgtggt gacctcggcg cggagcttca attcggaagc
     1801 aacctgggct tccttgtaga aggtgccgct cttgggcgag agatcggtgg tggcggtcac
     1861 ggtttcatcc gctgcgttcg cgttggcgac cagcgcgaag gggacgacca tcgcgatcac
     1921 gagggctatg tatttcttca ggggattcat ttctttgcct caatcatgtg gagtgttgga
     1981 gagaacggac gatccgcgcc tgtgtgcggg ccgtcaccta taaatacccg ccgaaaacgg
     2041 ctttgtttcg ccatttacga aacatttagg gcacccaaag caaatcggcc ggagggctcc
     2101 tgaaagccct ccggccgact ttggtacgcg gaatctaccg ggtcagcgac gaaggatgcg
     2161 accccaggcc accgtcttcg tggcgcggcc gttcttcggg ttgatgcgga agacgatctt
     2221 cgccttgctg cccttgcggc cagttacccg cgcctttacc ttggtccagc gagtctggcc
     2281 gggccagatg ttggccatcc gggagctgcc gcgggcgctt ccgtacgcct tcaccttgac
     2341 gcccctggcg accgcggtac cggtgttctt gacctttacc ttgaatacct tgcgcgaccc
     2401 cctccggagg cgcttgggcc cgtaggctct cgccttcttc aaccgcgggt tgcccttgag
     2461 ccccttgcag ttcatcgtga acggcgtcgc cttcacgatg gtttcgggac cggtgtctgt
     2521 gccgccgggg aaggcccgtt cacccagggt gaaagttccg tcggtggtcc acttgccgct
     2581 cgagcatttt gaccgggcgt agttcgggtc gagacccttc acggtctccg taccgttgtc
     2641 ggtctccacc tggatgcccg aaccggggat gtcc
//
LOCUS       DAOTIP010000246         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45362_length_2672_cov_3.507069, whole genome shotgun sequence.
ACCESSION   DAOTIP010000246 DAOTIP010000000
VERSION     DAOTIP010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45362_length_2672_cov_3.507069"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5138_05520"
     CDS             <1..830
                     /locus_tag="P5138_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRV60068.1"
                     /translation="NGPTWLDGDTVASPHSYEVALRSAGASLVAVDAVTGPEKSADSL
                     FAIIRPPGHHATADRAMGFCLFSNAAVAARYAREQLGIDRVAVLDWDVHHGNGTQDIL
                     YDDPSVLFVSFHQWPLYQGRCWNDEVGVGEGRGFTVNLPMPPGSGDLEHIEGFNAIAG
                     PILDQFNPGLLIVSAGQDGHVADELASQNLTAAGYHGLAQASAEFAASRGIGLVALHE
                     GGYNPNTLPLLDHAIYAGIGGFAPDLAGDAPAPETEEIGWHERLVQIRRIQSAYWAV"
     gene            complement(845..1093)
                     /locus_tag="P5138_05525"
     CDS             complement(845..1093)
                     /locus_tag="P5138_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60069.1"
                     /translation="MNRVRNSVVAILTALFVLAMPAFAAAADGVGTAGRVDDRYITFF
                     CFGVIAFFAILVTVLSLIQGRLDAKKDQRRHDLDRFNS"
     gene            1466..2260
                     /locus_tag="P5138_05530"
     CDS             1466..2260
                     /locus_tag="P5138_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV60070.1"
                     /translation="MSAPRTPLRVAVIDADSGFIQVLSNRMDSVGWQYRILASGLPPE
                     ELVAMKINAVLLDPEVVGEGGWDYLEKICGLLPDLGVIVCANGGTVSQRVRGLRIGAD
                     DWINKPCHPEEVIARIEAVVRRRRRHSNEQESGPLVIGEIEIRSDQFQAFISGQSLDL
                     TRREFELLQLLGQNEGKVLDREAIYQRVWGYAMAHGDRSVDVFIRKLRTKLEKRSPEY
                     TYIHTHFGVGYRLAAESSMSVADAAVEVGSDRATEDTGAEAPGVPV"
     gene            2443..>2672
                     /locus_tag="P5138_05535"
     CDS             2443..>2672
                     /locus_tag="P5138_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRV60071.1"
                     /translation="MTDKFRIQYQEELDHLERQALGAFDLVSRALDRTLEAIEHQDVE
                     LAEIVATDDDRIDGRYLEIHQGLITLLATQSPV"
BASE COUNT          495 a          853 c          847 g          477 t
ORIGIN      
        1 agaacggacc gacctggctg gacggcgaca cggttgcctc gccgcatagt tacgaggttg
       61 cccttcgctc cgccggggca tccctcgtcg ccgtggacgc ggtaaccgga ccggagaaaa
      121 gcgctgacag cctgttcgcg atcattcggc ctcccggcca tcacgccacc gccgaccggg
      181 caatgggatt ctgtctcttc agtaacgccg cggtcgcggc ccgttatgcc cgcgagcagc
      241 tggggatcga ccgggttgcc gtactcgact gggacgtgca ccatggcaac ggcacccagg
      301 acatcctcta cgacgacccg tcggttctct tcgtctcctt ccaccagtgg ccgctctatc
      361 agggccgctg ctggaatgac gaggtcgggg tgggggaggg ccgcgggttc acggtcaacc
      421 tgccgatgcc gcccgggtca ggtgacctcg agcacatcga aggcttcaat gcgatcgcgg
      481 gcccgatcct cgaccagttc aatccgggac tgctgatcgt ctcggccggt caggacggtc
      541 atgtcgcgga tgagctcgcc agccagaacc tgactgcggc cggctatcac ggtctcgccc
      601 aggcctcggc ggaattcgcg gccagccggg gtatcggcct ggtcgccctc catgagggcg
      661 gctacaaccc gaacaccctg ccgcttctcg accatgccat ctacgcgggc atcggcggct
      721 tcgccccgga cctggcgggt gacgccccgg cccccgagac ggaggaaatc ggctggcacg
      781 aaagactggt tcagatcagg cggatccagt ccgcctactg ggccgtctga agccggttcg
      841 gtttctagga attgaaccgg tcgagatcgt gccggcgctg atccttcttc gcgtccagcc
      901 gtccctggat cagggagagg acggtgacga ggatcgcgaa gaaggcgatc acgccgaagc
      961 agaagaaggt gatgtaacgg tcgtcgaccc ggccggcggt gccgacacca tcggcggccg
     1021 ccgcaaaggc gggcatggcg agcacgaaaa gggcggtcaa gatggcaacg acgctgttgc
     1081 ggacacgatt cacgccccga aacctaccgc aatctgccgg acgttggtcg aagggccccg
     1141 ggccttagaa cccgtcgacc gccaaccgtt gtcgtgggtg gcgcaaggtt gtgaaggtcc
     1201 ggtgtgggtg tgtgcgcaag aaacctcgga atggggagcg aagcgacggt ttcttgggta
     1261 tacacccaca ccggacccat cgaccaatac cggccccacg gcttcacacc tcgatccggg
     1321 aattgtgagc catttcacac ctccgtttcg aggcccagag ccgaatctcc gggggcttgt
     1381 tattgacttg tgaatttcgg cctgttttcg ggccttggcg tccgaagact ccctagactt
     1441 tgcagtagat gatcggaagg tctaaatgag tgcccccagg actcccctcc gcgtcgcggt
     1501 gattgacgcc gattccggat tcatccaggt cctttcgaac cgcatggact cggtcggctg
     1561 gcagtaccgc atccttgcct cgggcctgcc gccggaggaa ctggtggcga tgaagatcaa
     1621 cgccgtgctg ctcgacccgg aagtggtcgg cgagggcggc tgggactacc tcgagaagat
     1681 ctgcggcctt ctgcccgacc tcggcgtgat tgtctgcgcc aacggaggca cggtctccca
     1741 gcgggttcgc ggtctcagga tcggcgccga cgactggatc aacaagccct gccatccgga
     1801 ggaagtgatc gccaggatcg aggcggttgt ccggcggcgc cggcgccact ccaacgaaca
     1861 ggaatccggg ccgctcgtga tcggggagat cgaaatccgt tccgaccagt ttcaggcttt
     1921 catcagcggc cagagcctcg atctcacccg ccgcgagttc gagttgcttc agttgctcgg
     1981 gcagaacgaa gggaaggtcc tcgaccgtga ggcgatctac cagcgggtct ggggctatgc
     2041 gatggcccac ggcgaccgct cggtcgatgt cttcatccgc aagctccgta ccaagctgga
     2101 gaagcgttcc cccgaataca cctacatcca cacccatttc ggcgtcggct acagacttgc
     2161 ggccgaatcg tcgatgtcgg tcgctgacgc ggccgttgag gtcggctccg accgggccac
     2221 cgaagatacg ggcgccgagg cgcccggcgt gccggtctag cgctgcaccg ctctcactca
     2281 agaaacgggc cgcccccacc aagggcgatc ccgcttcacc cgagttcaca actcttttac
     2341 agcaggtcta cccggaagta acaaacttgt gttgaagtgg tggaacgctt cgaaccgtca
     2401 gaccgcccgt gaaagtggcg gggaatatcc cggagggtgt gagtgaccga caagttccgt
     2461 atccagtatc aggaagaact cgatcatctc gagcgtcagg cgctgggcgc attcgatctc
     2521 gtatcgcgtg ccctggaccg cacccttgag gcgatcgagc atcaggacgt agagttggca
     2581 gagatcgtcg caacggatga cgaccggatc gacggccgtt acctggagat ccatcaggga
     2641 ctgatcaccc ttctcgccac ccagtcgccc gt
//
LOCUS       DAOTIP010000247         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45458_length_2668_cov_3.181018, whole genome shotgun sequence.
ACCESSION   DAOTIP010000247 DAOTIP010000000
VERSION     DAOTIP010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45458_length_2668_cov_3.181018"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="P5138_05540"
     CDS             complement(<1..577)
                     /locus_tag="P5138_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60072.1"
                     /translation="MNEESNGIEVEGLVRQFKNGPRAVDGISLTVEPGEIYGFLGPNG
                     AGKSTTVHMLTTLLPPTAGSARVAGLDVATRGAEVRKVIGAALQEAALDPNLTGREHM
                     KLQTALHGLPKEERVRRGDELIERVGLSDASDRRVGGYSGGMKRRLDLALALVHRPRV
                     LFLDEPTTGLDPQSRASLWEEVTRLSTRDGVT"
     gene            633..1886
                     /locus_tag="P5138_05545"
     CDS             633..1886
                     /locus_tag="P5138_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV60073.1"
                     /translation="MTESTTAPIDLFEKVKNHERREQLEAANEGGLNPYFRLLTSQAG
                     PVVEMEGRETIMLGSNNYLGLTGDDRVKNAAREALDQYGTGVTGSRLLNGTTPIHVEL
                     EEQLAGWMNTEDAIVFSTGYQANVGCLQAMLGPADTVICDSGDHASLLDGCKLSGAKL
                     RPFRHNQMDKLEKMLQRAAGDGGGVLVVVDGVYSMEGDLPDLPRVVELSKHYGARIMV
                     DEAHGVGVLGERGAGATELFGLEDEVDLRMGTFSKSLASCGGFIAGDHDVVDYMRITS
                     RSFIFSASGVPAAVGAALEATKICRSEGAPLFARLLENARYLRHGLEDLGLKVVQPGT
                     MPDGSVADTPVVPVVVGEDWTAVLLWKALFDAGVYCNVAIHPAVPPSGALLRTSLMAT
                     HEKEQLDKALGIISEVIKDFPTLTE"
     gene            complement(2080..>2668)
                     /locus_tag="P5138_05550"
     CDS             complement(2080..>2668)
                     /locus_tag="P5138_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV60074.1"
                     /translation="EWDPEAKNYTALMNKVKKSNPDAILLAGLTEQNGGQLIKDKVSV
                     LGPNDGEVGLMAPDGFAQQSTIDEAGAASAGMIASVPGRTPELLPGTGKQFVNQLKSK
                     TGNQPVELYAPYAGQAAQVLLDAIDSAGNERGAVASALYGMKITDGITGSFTITDTGD
                     LDVSPITISKAGTEFEPVEVVEPKAKLINAARGQS"
BASE COUNT          421 a          923 c          866 g          458 t
ORIGIN      
        1 cggtcacccc gtcccgggtc gagagcctcg tcacttcctc ccagagcgaa gcgcggctct
       61 gcgggtcgag gccggtggtc ggctcgtcga ggaagaggac tctcggccgg tgaaccaggg
      121 ccagtgcgag atcgaggcgg cgcttcatgc cgcccgaata tccgccgacc cggcggtcgg
      181 aggcgtcgga aaggccaacc cgctcgatca gctcgtcgcc gcgacgtacc cgttcctcct
      241 tgggcagccc gtggagggcg gtctgcagct tcatgtgttc gcgcccggtc aggttcggat
      301 cgagcgccgc ctcctgcagg gcggcaccga tcaccttccg cacctcggcc ccgcgggtcg
      361 cgacgtcaag cccggcgacc cgggcggatc ccgcggtcgg gggcagcagg gtggtcagca
      421 tgtgcacggt ggtcgacttg cctgcgccgt tcggcccgag gaagccgtat atctcccccg
      481 gctcgacggt caggctgatc ccgtcaaccg ccctcggccc gttcttgaac tggcggacga
      541 gcccttcaac ttcgattccg ttgctctctt cgttcatcga tggctatgta tacgcgatcg
      601 gcctcacggg gccggcgaat tacactttcg gcatgaccga gagcacgaca gcaccgatcg
      661 acctcttcga gaaggtcaag aaccatgagc gccgcgagca gctcgaagcc gccaacgaag
      721 gcgggctgaa cccctacttc cgcctgctca cctcacaggc cggtcccgtg gtcgagatgg
      781 aaggccggga gacgatcatg ctcggctcga acaactacct cgggctgacc ggtgacgacc
      841 gggtcaagaa tgcggcccgg gaggcgctgg accagtacgg caccggggtc accggttcgc
      901 gcctcctgaa cggcaccacc ccgatccacg tcgagctcga ggagcagttg gccggctgga
      961 tgaacaccga ggacgcgatc gtcttctcga ccggctacca ggccaacgtc ggctgcctgc
     1021 aggcgatgct cgggccggcc gacaccgtga tctgcgactc cggggaccac gcctccctgc
     1081 tcgacggctg caaactttcc ggggcgaagc tccggccctt ccgtcacaac cagatggaca
     1141 agctggagaa gatgctccag cgcgcggccg gcgacggcgg cggcgtcctg gtggtcgtcg
     1201 acggcgtcta ttcgatggag ggtgacctgc ccgacctgcc ccgggtggtc gagctctcga
     1261 agcactacgg cgcccggatc atggttgacg aagctcacgg cgtgggcgtg ctcggcgagc
     1321 gtggcgccgg tgccaccgag ctgttcggac tggaggacga ggtcgacctc aggatgggca
     1381 ccttctccaa gagcctcgcc tcctgcggcg gcttcatcgc cggcgatcac gacgtggtcg
     1441 actacatgcg gatcacctca cgctcgttca tcttcagcgc ttccggtgtg cccgccgcgg
     1501 tcggagccgc actcgaagcg acgaagatct gccggagcga aggcgccccg ctgttcgccc
     1561 gtctgctcga gaacgcccgc tacctccgcc acggcctcga ggacctgggc ctcaaggtcg
     1621 tccagccggg caccatgcct gacggctccg tcgcggacac tccggtcgtg ccggtcgtgg
     1681 tcggtgagga ctggaccgca gtgctgctct ggaaggcact cttcgacgcc ggggtctact
     1741 gcaacgtggc gatccacccg gcggtgccgc cgtcgggagc cctgctcagg accagcctca
     1801 tggccacaca cgaaaaggaa cagctcgaca aggccctggg catcatctct gaggtcatca
     1861 aggacttccc caccctcacc gaataggccc gggcgctccc ggaggccctc aggacgacgc
     1921 tgccttcgtc aatcgccccg gaagaacgga tcccggagcg tggcgtcaaa gcttgggccg
     1981 gatggattcg gaggtggcgc aaggttgaac ggatcccggc gtgccggccg acgacgtttc
     2041 gggtcgagga gaccggtacg ccgggatccg ttccctcgcc tagctctgac ctctggcggc
     2101 gttgatcagc ttcgccttcg gctcgaccac ttcgaccggc tcgaactccg tgccggcctt
     2161 gctgatcgtg atcgggctga cgtccagatc accggtgtcg gtgatcgtga agctgccggt
     2221 gatcccgtcc gtgatcttca tcccgtagag ggctgacgcc acggcgccac gctcattgcc
     2281 cgccgaatcg atggcatcga gcaggacctg tgccgcctgg cccgcgtagg gcgcgtagag
     2341 ctccacgggc tggttgccgg tcttcgactt gagctggttg acgaactgct tgccggtgcc
     2401 cggcagcagc tccggcgtac gacccggcac cgaggcgatc atcccggccg aagccgcgcc
     2461 ggcctcgtca atcgtcgact gctgcgcgaa gccgtccggc gccatcagtc cgacttcacc
     2521 gtcattcgga cccaggaccg aaaccttgtc cttgatcagc tggccgccgt tctgctcggt
     2581 aaggccggcc agaaggatcg cgtccggatt gctcttcttc accttgttca tcagggccgt
     2641 gtagttcttc gcctccgggt cccactcg
//
LOCUS       DAOTIP010000248         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45464_length_2668_cov_2.868733, whole genome shotgun sequence.
ACCESSION   DAOTIP010000248 DAOTIP010000000
VERSION     DAOTIP010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45464_length_2668_cov_2.868733"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5138_05555"
     CDS             <1..659
                     /locus_tag="P5138_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60075.1"
                     /translation="AEETLITSGLTAGEGSVGTIGSTAEWDAIPEPEEDPLGEPVAEE
                     GADIHIFKPRVVGGTTVDSAEDPLFGIVPEFQPEPIPEAPLQPMPEIERALGIEPSSE
                     PSGEPEFVVSSRSNGNGKGLFDQDGSGEETGLTRLLEPVPQEGEISVTASTEIGSTLR
                     EDWLSQSQGSGSTMEWPSHAMDGLDEARHNPHPKPNPNFFPEPETTEWSVSEMRFKHR
                     "
     assembly_gap    723..732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(940..2091)
                     /locus_tag="P5138_05560"
     CDS             complement(940..2091)
                     /locus_tag="P5138_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRV60076.1"
                     /translation="MASVESIIEGREIVICAGSGGVGKTTTSAAIALGMAARGLKVCV
                     LTIDPAKRLADSLGLKELGNEASKVNPKLLREQGVNPEKGGELWAMMLDAKETFDALV
                     ARHAEDAEARDRVLNNRIYQQISGALAGSQEYMAMEKLFELHSEGRFDLLVLDTPPSR
                     NALDFLDAPQRLTQFIEGKSLQIFIRPTGFAARVASRGTGVALAVLKRIVGFDLLADL
                     SEFFTAFSGMIDGFQDRAHRVSSLLADERTCFLVICGPQGQPIDEAVFFHSKLVENRM
                     NFGGAVVNRVRYRLGTQKPKVKDLARRIDSELDDEDLSERLARTLAEYDVLAVRDQKN
                     IQVLTDTLSGEPVIRIPYLDTDVHDIAGLCQINEYLFIDEEQRVEKAIS"
     gene            complement(2091..>2668)
                     /locus_tag="P5138_05565"
     CDS             complement(2091..>2668)
                     /locus_tag="P5138_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRV60077.1"
                     /translation="LVTIGKIWELAQLDRKVKHGRKYDLVIVDAPATGHGISFLQTPR
                     TFASIARVGPIHSQALQLEELITDHEQTGTVIVSLPEEMPVNETARLEAELSGDIGIS
                     IDRVIMNALYPENFSKGEVKKLSKLRKSDDDEVRAAGRAGTSQATRAADQREQLARLE
                     EMVETEVTTLPFIFKPELDLKSARKLAEEIT"
BASE COUNT          439 a          873 c          866 g          480 t
ORIGIN      
        1 ccgccgaaga aaccctgatc accagcggtc tcaccgccgg tgaaggcagc gtcggcacga
       61 tcggctcgac cgcggaatgg gacgcgatcc ccgagccgga agaggatccc cttggcgagc
      121 cggtcgctga agaaggcgcc gacatccaca tcttcaagcc ccgcgtggtc ggcggcacca
      181 ccgtcgattc ggccgaggac ccgctcttcg gcatcgttcc cgagttccag ccggaaccga
      241 ttccggaggc gccgcttcag ccgatgccgg agatcgagcg tgccctcggc atcgaacctt
      301 catccgagcc gtcaggcgag cccgaatttg tggtctcgtc ccggtcgaat ggcaacggga
      361 aagggctctt cgaccaggac ggcagcggcg aggaaaccgg attgaccagg ctgctcgagc
      421 cggtgccgca ggaaggcgag atctcggtca ccgcatcgac ggagatcggt tcgaccctgc
      481 gcgaggactg gctctcccag tcacagggca gcggctcgac catggaatgg ccttcgcacg
      541 cgatggacgg actcgacgaa gcccgccaca atccccaccc gaagccgaat ccgaacttct
      601 tccccgagcc ggagaccacc gagtggtcgg tctccgagat gcgcttcaag caccgctgac
      661 ccgggcggcg ccaggatcca ccggttcgct ggtggtgacg ccgcggggcc ggatggccag
      721 gcnnnnnnnn nnggccgtgg gcccgggcga gcatcgccgg gaagcggcaa acctcgggcg
      781 gcattcagcc gccctccgcc tgagtatccc ggcgacgctc acccggaccc acggcaaccg
      841 gaaccattgg cccggaacga cggtgccgca ggtttgcttc ctctgcggtc gtgaacctgc
      901 ggcccccgcg gcgtcaccac ccggcggatt cgccgggggt caggagatgg ccttttcgac
      961 ccgctgttct tcgtcgatga acaggtactc gttgatctgg cagaggcccg cgatgtcgtg
     1021 gacgtccgtg tccaggtagg ggatacggat caccggttcg ccgctcaggg tgtcggtgag
     1081 cacctggatg ttcttctggt cccggaccgc gaggacgtcg tattcggcca gggtcctggc
     1141 cagccgttct gacaggtctt cgtcatcgag ctccgaatcg atcctccttg ccaggtcctt
     1201 gaccttcggc ttctgggtgc cgagccggta gcggacccgg ttgaccacgg cgccaccgaa
     1261 gttcatccgg ttctcgacca gcttcgaatg gaagaagacc gcttcgtcga tcggctggcc
     1321 ctgcggtccg cagatcacca ggaagcaggt gcgttcatcg gccagcagcg aactgacccg
     1381 gtgggcccgg tcctgaaaac cgtcgatcat gccgctgaag gcggtgaaga actcggacag
     1441 gtcggcgagc aggtcgaagc cgacgatgcg tttcaggacg gcaagggcga ccccggtgcc
     1501 acggctggcg acccgggcgg cgaagccggt cggccggatg aagatctgga gtgacttgcc
     1561 ctcgatgaac tgggtgagcc tctgcggggc gtcaaggaaa tcgagagcgt ttcggctcgg
     1621 cggcgtgtcc aggaccagca ggtcgaagcg accttcggaa tggagttcga agagcttctc
     1681 catcgccatg tattcctgcg agccggccag cgcgccggag atctgctggt agatccggtt
     1741 gttcaggacc cggtccctgg cctcggcgtc ttcggcatgc ctggcgacca gggcatcgaa
     1801 agtctccttc gcgtccagca tcatcgccca gagttcgccg cccttctcgg ggttcacccc
     1861 ctgctcccgg agcagtttcg gattgacctt gcttgcctcg ttgccgagtt ccttcaggcc
     1921 gagcgaatcg gccagacgct tggccgggtc gatggtcaac acacagacct tcaggccccg
     1981 cgcggccatt ccgagcgcga tcgcggccga ggtggtcgtc ttgccgaccc cgccggatcc
     2041 agcacagatc acgatttcgc ggccttcgat aatcgattcg accgaggcca tcaggtgatc
     2101 tcctcggcca gcttgcgggc cgatttcagg tcgagttccg gtttgaagat gaaggggagg
     2161 gtggtcacct cggtctcgac catctcctcg agtctcgcca actgctcccg ctggtcggcg
     2221 gcccgggttg cctgggatgt cccggccctc ccggccgccc ggacttcatc gtcgtcgctc
     2281 ttcctgagct tcgagagctt cttcacctca cccttcgaaa agttctcggg atagagcgca
     2341 ttcatgatga cccggtcgat cgagatcccg atgtccccgg agagctccgc ctcgagtctc
     2401 gccgtctcgt tgaccggcat ctcctcgggc agcgagacga tcaccgtccc ggtctgctcg
     2461 tggtcggtga tcaattcctc gagttgcagg gcctggctgt ggatcgggcc gacccgggcg
     2521 atcgaggcga aagtgcgcgg ggtctgaaga aagctgatgc cgtggccggt cgccggggca
     2581 tcgacgatga ccaggtcgta cttgcggccg tgcttgacct tgcggtcgag ctgggcgagc
     2641 tcccagatct tgccgatcgt gaccagtt
//
LOCUS       DAOTIP010000249         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45502_length_2666_cov_3.581769, whole genome shotgun sequence.
ACCESSION   DAOTIP010000249 DAOTIP010000000
VERSION     DAOTIP010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45502_length_2666_cov_3.581769"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="P5138_05570"
     CDS             <1..260
                     /locus_tag="P5138_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60078.1"
                     /translation="GGGGGNGGSSGGSGGSGGGGGGGSTGGSTGQNPTTQPTDPVEPT
                     NPSTPQPTDPVPDPNPDPNPNPNPIPGGPGSITTPGGPTPG"
     gene            complement(271..963)
                     /locus_tag="P5138_05575"
     CDS             complement(271..963)
                     /locus_tag="P5138_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRV60079.1"
                     /translation="MSPAATRKSEALPTAAVEDYAKAIYSLTGWGEETASTNDLAERL
                     GVKAGSVSAMIKKLDEAGLVERVPYHGVRLTADGTRVAMAVLRRHRLLELFLAEVLDV
                     PWERVHDEAEVLEHALSEDLTDLISAKLGEPDFDPHGDPIPDRDLEIVETETVALAGL
                     APGDVARFVRVSDSNPEMLAYLSECGIAVGDRLELVERQPFDGPITVKIGDASHVLGL
                     TLAQAMRVDHTD"
     gene            1039..>2666
                     /gene="glsA"
                     /locus_tag="P5138_05580"
     CDS             1039..>2666
                     /gene="glsA"
                     /locus_tag="P5138_05580"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HRV60080.1"
                     /translation="MNQPANSRTSSPVQALLEQLHERHAGDMSGEVASYIPELASADP
                     AWFGISMVTADGAVYEVGDTRREFTIQSISKPLTYALAQEAIGEEEVRRHVDVEPSGE
                     AFNSITLQPGTGLPLNPMVNAGAIATTSLIEPIGFEKPIDRILHEYSEFAGRHLKVDE
                     EVFNSENETGHRNRAIAYLLKNAGVVDREVEEVVETYFRQCSTLVTCRDLATIAATLA
                     STGVNPVTGERVLREETVRNVLSVMASCGMYDAAGDWLYTVGLPAKSGVAGGIIAVLP
                     GQLGFAVYSPPLDAHGNSVRGIKVCEDLSEVLHLHVMSPSRRPAPPVRAEGSCLDSQS
                     KRFRGPAEQNILAEHGRRARVLQLQGEISFAAGELITGSALERAEHHDFTVLDFRAVR
                     SIDPIDIPIFSHLIESCEEAGGEIAFSDVDRHPLFLEDLGRHREQEGLHPVRTFPDLD
                     LALEWCEQGLIDRYGDLETDPELSLAQHGATLGMSIDEIELLSSHLEHVTFGAGELIY
                     GDEMVEPEEMLLVTAGKISLLARADDGGFQRLATL"
BASE COUNT          468 a          887 c          876 g          435 t
ORIGIN      
        1 gcggtggcgg tggcggcaac ggggggtcct ccggcgggtc cggcgggtcc ggtggcggcg
       61 gaggcggtgg aagtacaggc ggttcgaccg gccagaaccc gaccactcag ccgaccgatc
      121 cggttgagcc gaccaatccg agcacccctc agccgaccga ccccgtgccc gacccgaacc
      181 cggatccgaa tcccaacccg aatccgatcc ccggcggacc ggggagcatc accactcctg
      241 gcgggccgac gcccggctag cgtcacgcct tcagtcggtg tgatcgaccc gcatcgcctg
      301 ggcgagggtc agcccgagca catgcgatgc gtccccgatc ttcacggtga tcgggccgtc
      361 aaacggttgc cgttcgacca gctcgagccg gtcgccgacc gcgatcccgc actcgctcag
      421 gtaggcgagc atctccgggt tggagtcgga gacccggacg aaacgggcga cgtcgcccgg
      481 cgcgagcccg gctagggcga ccgtttcggt ctcgacgatc tccagatccc gatccgggat
      541 cgggtcgccg tgcgggtcga agtccggctc acccagcttg gccgagatca ggtcggtgag
      601 gtcttcggag agcgcgtgtt cgagtacctc cgcctcgtcg tggacccgtt cccagggaac
      661 gtcgagaacc tcggcgagga agagttccag caaccgatgc cggcgcagta cggccatcgc
      721 caccctggtc ccgtcggcgg tgagcctgac cccgtggtag ggaacccgct cgaccagtcc
      781 cgcctcgtcg agtttcttga tcatcgccga gaccgatccg gccttgaccc cgaggcgttc
      841 ggcgagatcg ttggtggaag cggtttcctc gccccagccg gtcagcgagt agatcgcctt
      901 cgcgtaatcc tcgaccgccg cggtcggcag cgcctcggac tttctggtgg cagccggact
      961 cacaagttga gcctagccaa aattcgttcg gacctcgtga ctggaaggtg agcgggatgg
     1021 ggtatagaag cagcaaccgt gaaccaacca gctaattccc gcacctcctc gcccgttcag
     1081 gcgttgctcg agcagcttca tgagcggcat gcgggggata tgtccgggga ggttgcgagc
     1141 tatatccccg agttggcttc ggccgatccg gcctggttcg ggatctcgat ggtcaccgct
     1201 gacggcgccg tctacgaggt gggggacacg cgacgcgagt tcacgatcca gtcgatttcg
     1261 aagccgctga cctacgcgct cgcccaggag gcgatcggcg aagaagaggt ccgacggcac
     1321 gtggacgtcg agccgagcgg cgaggcgttc aactcgatca ccctgcagcc gggcaccgga
     1381 ctgccgctaa acccgatggt gaacgccgga gcgatcgcga ccacttcgct catcgagccg
     1441 atcggattcg agaagccgat cgaccgcatc ctccatgagt actcggagtt cgcgggcagg
     1501 cacctcaagg tggacgagga agtcttcaac tccgagaacg agactggcca tcgcaaccgg
     1561 gcgattgcct acctgctcaa gaacgccggg gtggtcgacc gcgaagtcga ggaggtggtc
     1621 gaaacctact ttcgccagtg ctcgaccctg gtcacctgcc gggacctggc cacgatcgcc
     1681 gcgaccctcg cctcgaccgg cgtgaatccg gtgaccggcg aacgcgtatt gcgggaggag
     1741 acggtgcgta acgtgctcag cgtgatggcc agctgcggca tgtacgacgc agccggggac
     1801 tggctttaca cggtgggcct gcccgcaaag tcgggtgtcg cgggagggat cattgcggtc
     1861 cttcccggcc agctcgggtt cgccgtctac tcgccgccgc tcgatgccca cggcaacagc
     1921 gtccgcggga tcaaggtctg cgaagacctt tccgaggtgc ttcacctcca tgtgatgagc
     1981 ccgtcgaggc ggccggctcc gccggtgcgg gccgaaggct cctgcctgga ctcgcagtcg
     2041 aaacgcttcc ggggccccgc tgaacagaac atcctggccg agcacggacg ccgggccagg
     2101 gtcctccagc tgcagggaga gatctcgttc gccgcaggag agctgatcac cggttcggcg
     2161 ctcgagcggg ccgaacacca cgacttcacg gtcctcgatt tccgggcggt ccgctcgatc
     2221 gacccgatcg acataccgat cttcagccac ctgatcgaga gttgcgagga ggccggtggc
     2281 gagatcgcct tctccgacgt cgaccgccat ccgctcttcc tcgaggacct gggccgccac
     2341 cgggagcagg aaggactaca tccggttcgc acctttcccg atctcgatct ggcgctcgaa
     2401 tggtgcgaac agggacttat cgacaggtac ggtgacctcg agaccgaccc cgaactcagt
     2461 ctcgcccagc acggtgccac cctcggcatg agcatcgacg agatcgaact gctgagctcg
     2521 catctcgaac atgtgacttt cggggcgggg gaactcatct atggggacga aatggtcgag
     2581 cccgaggaaa tgctcctggt caccgccggc aagatcagcc tgctggccag ggccgacgac
     2641 gggggattcc agcgcctcgc caccct
//
LOCUS       DAOTIP010000250         2661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45618_length_2661_cov_4.447813, whole genome shotgun sequence.
ACCESSION   DAOTIP010000250 DAOTIP010000000
VERSION     DAOTIP010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45618_length_2661_cov_4.447813"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1195
                     /locus_tag="P5138_05585"
     CDS             29..1195
                     /locus_tag="P5138_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60081.1"
                     /translation="MDGPVRVASKIQGSLALGFAIVALILKGPLENGLLSGNSTLYWV
                     YFSSVLFYAASYFARGYLAGHQQFGLFTTLILSESVFRTMFAALVAVGILSGQSMVAL
                     GIVAAPLLSLMVVPPAITRRSRREAEERAAYAESHGLAAEDVHAEEVEAREEFSLSHG
                     AGFVGSVFLIMFSEQVFLNAGPLVVRAVEGVAAAGYIFNVLMIARAPLQLFQAVSTSI
                     LPHLTALHHSKDPDSERQFHHSVRMVLLGIFAFTAVSFAVMAVAGPKLMQIAFSDKFT
                     YDRLGLLLVVVGMGLYLSAVTVNQACLAQGQARRSSIRWISCAVFFIVWTILPFISDG
                     NLRVEIGFALTALLLFALLTFIYLRPKGIEEDVPLEGSSEAIEAKLAGLEDTSP"
     gene            complement(1212..1925)
                     /gene="rpe"
                     /locus_tag="P5138_05590"
     CDS             complement(1212..1925)
                     /gene="rpe"
                     /locus_tag="P5138_05590"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRV60082.1"
                     /translation="MTKQPRTAETPVPESFQGIRIAPSILSADFGKLGSQVGEVMEAG
                     AKVIHFDVMDGQFVPPITIGPLVLAGIADQVHDAGGVVDVHLMIENPDRQFEDFAKAG
                     ADSIIFHEEATPHAHRCCHAIRELGCLAGVAINPGTPVEAVSELKEMADEILVMTVNP
                     GWGGQPFIETSPDKVKRMRSLVGDDVLIEVDGGIGTGTAGTMAEAGATLMVAGSAIFG
                     TEDPAQAYKDIAAAAEAGA"
     gene            complement(1943..2182)
                     /locus_tag="P5138_05595"
     CDS             complement(1943..2182)
                     /locus_tag="P5138_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60083.1"
                     /translation="MATESEKTTVDRRPPCPGCGEPWLRPTQLPGRFRCVYCLARYEL
                     VSQCPNCGEHQTIARMSTTEDMTCQSCGASMLQPI"
     gene            complement(2263..>2661)
                     /locus_tag="P5138_05600"
     CDS             complement(2263..>2661)
                     /locus_tag="P5138_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60084.1"
                     /translation="DARESEILSGFDRVLVDAPCSDLGALASRPDARWRKSPALIERV
                     ADLQREILDRATGFIREGGSLVYSTCTISKRENSDQAIELAERQRLEIVDLGARSPGL
                     ADPHDRRFLQLMPDRDHTTGFFIARFTSPG"
BASE COUNT          393 a          897 c          857 g          514 t
ORIGIN      
        1 cgctcggtga gttcttccgt ctcgggggat ggacggcccg gtccgcgtcg cgtccaagat
       61 ccagggctcc ctggcgctcg gcttcgcgat cgtcgccctg atcctcaagg gcccgcttga
      121 gaacggcctg ctctccggga attcgaccct gtactgggtc tatttctcgt cggtcctctt
      181 ctacgccgcc agttacttcg cccgcggcta tctggccggc catcagcaat tcggcctctt
      241 cacgacgctg atcctctccg agtcggtgtt ccggaccatg tttgcggcgc tggtcgcggt
      301 tggcatcctt tccggccagt cgatggtggc gctcggcatc gtcgccgcgc ccctgctcag
      361 cctgatggtg gtgccgccgg cgatcacccg ccgcagccgg cgggaagccg aagagcgggc
      421 agcctacgcc gaaagccacg gtctcgctgc ggaagacgtt cacgcggagg aggtggaagc
      481 ccgcgaggag ttctcgcttt cccacggggc cggcttcgtc ggctcggtct tcctgatcat
      541 gttctccgag caggtcttcc tcaacgccgg accgctggtc gtccgcgcgg tcgaaggggt
      601 ggctgcggcc ggatacatct tcaacgtgct gatgatcgcc cgggcgccgc tgcagctctt
      661 ccaggcggtc tcgacatcga tcctccccca cctcaccgcg ctccaccaca gcaaggaccc
      721 cgattcggag cgtcagttcc accactcggt ccgcatggtt ctgctcggca tcttcgcctt
      781 caccgcagtc agcttcgcgg tgatggccgt ggccggcccg aagctgatgc agatcgcctt
      841 ctcggacaag ttcacctatg accggctcgg actgctgctg gtcgtggtcg gcatggggct
      901 ctatctgtcg gcagtgaccg tgaaccaggc ctgtcttgcc cagggccagg cccggcgctc
      961 ctcgatccgg tggatctcct gcgccgtgtt cttcatcgtc tggacgatcc tgcccttcat
     1021 cagtgacgga aacctgcggg tcgagatcgg cttcgccctg acggcgctgc tgctcttcgc
     1081 cctgctcacc ttcatctacc tcaggccgaa gggcatcgag gaggatgtcc cgctcgaagg
     1141 ttcatccgag gcgatcgagg cgaagctggc cggcctcgag gacacttcgc cctaggtcgg
     1201 ttccaagggt ctcaggcgcc ggcttcggcg gcagcggcga tgtccttgta cgcctgggcc
     1261 gggtcctcgg tgccgaagat cgcggatccg gcgaccatca gggtcgcgcc tgcctcggcc
     1321 atcgtgccgg cagtgccggt tccgatcccg ccgtcaacct cgatcaggac gtcgtcgccg
     1381 accagcgagc gcatcctctt caccttgtcg ggagaagtct cgatgaacgg ctggccgccc
     1441 cagcccgggt tgaccgtcat cacgaggatc tcgtcggcca tctccttcag ttcggatacc
     1501 gcctcgaccg gtgttcccgg gttgatcgcg accccggcca ggcaaccgag ctcacggatc
     1561 gcgtgacagc agcggtgggc atggggggtc gcctcctcgt ggaagatgat cgaatccgcc
     1621 ccggccttcg cgaagtcttc gaactgccgg tccgggttct cgatcatcag gtggacgtcc
     1681 accaccccgc cggcgtcgtg gacctgatcg gcgatgccgg cgagaaccag cggaccgatg
     1741 gtgatcggag gcacgaactg cccgtccatc acgtcgaagt ggatgacctt cgctccagcc
     1801 tccatgactt caccgacttg agacccgagc ttgccgaaat cggcggaaag gatcgaaggt
     1861 gcgatgcgga tgccctggaa ggattcgggc acgggagttt cggcggttcg gggctgtttg
     1921 gtcatcggtg tctcggtcgg ggtcagatcg gttgaagcat cgaggcgccg caactctggc
     1981 aggtcatgtc ctcggtggtg ctcattctgg cgatcgtctg gtgttccccg cagttagggc
     2041 actgggagac cagttcatag cgcgccaggc agtacacgca gcggaaccgg cccggaagct
     2101 gggtcggcct cagccacggc tcaccgcaac ccgggcatgg gggtcgccgg tcgactgtgg
     2161 tcttctctga ctcggtcgcc atggggcgat tatcgcggac cgtccggctg ctccgcgcca
     2221 aaggcctttc ccggcgcctt caaccaccgg atgtgccggc ggtcaccccg gggaagtgaa
     2281 cctggcgatg aagaaacccg tggtgtgatc ccggtccggc atcagctgga ggaagcgccg
     2341 gtcatgcggg tcggccaggc cgggagaccg cgcaccgaga tcgacgatct cgagcctctg
     2401 ccgctcggcc aattcgatcg cctggtcgct gttctcgcgt ttcgagatcg tgcaggttga
     2461 atagaccagg ctgccaccct cccggatgaa tccggtcgcc cggtcgagga tctcccgctg
     2521 gaggtccgca acccgctcga tcagggccgg cgacttgcgc cagcgggcat cgggacgtga
     2581 ggccagtgct cccagatccg aacacggcgc gtcgaccagg acccgatcga agccgctgag
     2641 gatctcgctt tcccgcgcgt c
//
LOCUS       DAOTIP010000251         2661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45633_length_2661_cov_2.797774, whole genome shotgun sequence.
ACCESSION   DAOTIP010000251 DAOTIP010000000
VERSION     DAOTIP010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45633_length_2661_cov_2.797774"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="P5138_05605"
     CDS             <1..293
                     /locus_tag="P5138_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRV60085.1"
                     /translation="PPGGREMTFRPTVLKVETGRELRWRGRLLVPGIFDGEHCFRLTP
                     AGPGTRLEQGESFTGLLPRFMSVTLGQTERGFNELNQALKERAEARDPVADR"
     gene            290..769
                     /locus_tag="P5138_05610"
     CDS             290..769
                     /locus_tag="P5138_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HRV60086.1"
                     /translation="MSRREEITVAARALLEEEGEEALTMRSLAERLGIKAPSLYKHVS
                     GRQEIETLLAAEALTEMGETLANESGLEAMGRAYRTWALDNPALYRVATTRPLDRENL
                     TPGLEDAAAAPLLEALDGDRNRARAFWALAHGLVLLELDGRFPEDADLEAAWAAGLG"
     gene            complement(766..1158)
                     /locus_tag="P5138_05615"
     CDS             complement(766..1158)
                     /locus_tag="P5138_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV60087.1"
                     /translation="MSSLIHTCYRVFDLDESIGFYEKLGLKEHHRLPIGDEAVNVYMG
                     HEGDGPRLELTFNYDQEEPYEIGTGYGHIAFVVDDLDTTLDDLAVQGISPEKPPYTVS
                     EGGSRLCFVRDPDGYRIELIEGDPGRAD"
     gene            complement(1694..2131)
                     /locus_tag="P5138_05620"
     CDS             complement(1694..2131)
                     /locus_tag="P5138_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60088.1"
                     /translation="MSNYIQVPVPEQYVPRVYQLLAELDVQGDSNTTESSANESVVLD
                     EELVRRMYRESHENHRDLLKFMASRPDEWTPTGELLKALGYTSRKSASGLFGAFGKRA
                     NFRYGGAKPWIFAWEPGDVEARYMMTGEVSKWILDEASKEGNE"
     gene            2276..>2661
                     /locus_tag="P5138_05625"
     CDS             2276..>2661
                     /locus_tag="P5138_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60089.1"
                     /translation="MSTYERVAELPVEIDSWSMEGLSIKPAPDFERLTTVIRIEGGGH
                     AGLGEDITYEALDQIALQDAGPVHDLTGPKTIGEFCELIGSLDLFPSPPERETSRLYR
                     RWAFESAALDLALWQAGTSLAEFLARP"
BASE COUNT          494 a          860 c          788 g          519 t
ORIGIN      
        1 ggccgccggg cggccgcgag atgaccttca ggccgaccgt gctgaaggtc gaaaccggca
       61 gggaacttcg ctggcgaggc aggttgctgg tccccggcat cttcgatggc gagcactgct
      121 tccggctcac gccggccggg cctgggaccc gcctcgagca gggtgagtcc ttcaccggac
      181 tcctgccccg cttcatgagc gtgacgctgg gacagaccga gcggggcttc aacgaactga
      241 accaggcgct gaaggaacgg gccgaagctc gtgacccggt ggccgaccgt tgagccgccg
      301 cgaggagatc actgtggcgg cccgggcgct gctcgaagaa gagggtgaag aagctctgac
      361 tatgcgaagc ctggccgaac gcctcggcat caaggctccc tcgctctaca agcacgtgag
      421 tggccggcag gagatcgaaa cccttctcgc cgccgaagcc ctgaccgaga tgggcgagac
      481 actcgcgaac gagagcgggc ttgaggcaat gggccgcgcc tatcggacct gggccctgga
      541 caaccccgcc ctctatcggg tcgccaccac ccggcctctg gaccgggaga acctgacccc
      601 cggactcgag gacgccgccg cggcccctct gctggaagcc cttgacggcg accgcaacag
      661 ggcccgggcc ttctgggctc tggctcacgg cctggtcctg ctcgaactcg acggccggtt
      721 cccggaggat gccgacctcg aagcggcctg ggcggccggt ctgggctagt cggcgcggcc
      781 cgggtctccc tcgatcaact cgatccggta accgtcgggg tcacggacga aacagagcct
      841 cgacccgcct tcgctcaccg tgtacggggg cttctcgggc gagatgccct gcactgccag
      901 atcatcgagc gtcgtgtcga ggtcgtcgac cacgaaggcg atgtggccgt aaccggtgcc
      961 gatctcgtaa ggctcttcct ggtcgtagtt gaaggtgagc tcaagcctgg ggccgtcgcc
     1021 ctcgtgtccc atgtagacgt tgaccgcctc gtctccgatc ggaaggcgat gatgctcctt
     1081 gaggcccagc ttctcgtaga agccgatcga ttcgtccagg tcgaacacgc ggtaacaggt
     1141 gtggatcagg gaagacatgc ctcaaccgta cccgcttccc gggggaacga agcgaaacgc
     1201 atttcagacc gaacgaaccg gcgacctcgc gtcttgttgg tctatggacg tgtatctagg
     1261 cttatggccg cacaccgcag atttcacctg ttcaagatcg agtcgcctag ggcgttaaac
     1321 ccggcgtgaa attcggaatc ctcgaggaga tacgcccttt tccctttgaa ccttgaggca
     1381 tccgaaccaa tccgagctag tccaggacta agtggaacct ccgacgtaag cctgtagagc
     1441 tggacgggca ggtttcgttc ccgatcgact tcgccggtcg ccaagccggc agcgagcaga
     1501 cgcttttcgc cggccgcgca atagaggatc cgttcaccct tcttgatcgc gggtatgtct
     1561 tcaccagcga ccggccatct cgagaggagc tccgggcgcg aaccaaccca ctttcgatct
     1621 actctcgcct tttcttggcc catgcctacc gccaacagcc aattctgtga tctcttagcg
     1681 cctgaaccaa ccatcattca ttgccttcct tggaggcttc gtcgaggatc cactttgaaa
     1741 cctcgccagt catcatgtac ctcgcctcta cgtcgcctgg ttcccaagcg aaaatccaag
     1801 gcttcgctcc gccatatcga aagttggcac gcttcccgaa cgccccgaag agtccggaag
     1861 cgctctttcg gcttgtgtac ccgagcgcct tcagcagttc cccggtcggc gtccactcat
     1921 ccggacggga tgccatgaat ttgagcaggt ctcggtgatt ttcgtgactc tcacgataca
     1981 tgcggcgcac caattcttca tcaagaacga cagactcatt cgccgagctt tcggttgtat
     2041 tggaatcccc ttgaacgtcg agttcagcca acagttgata aaccctgggg acgtactgtt
     2101 cgggaactgg aacttgaata tagttgctca tttgctacct ccttagcttg tggtttttac
     2161 atatcctagc acgaaacact gtttcttgca gatttgccat tttttggcac tttggctgaa
     2221 ccggactcgg tttcctagcg tcggcgcaac caatcggggt cgttaggctg aatccatgag
     2281 cacctacgag cgcgtggcgg aacttccggt cgagatcgat tcgtggtcga tggaaggact
     2341 gtccatcaag cccgccccgg acttcgaacg cctgaccacg gtgatcagga tcgaaggcgg
     2401 cggtcacgcc gggctcggcg aggacatcac ctacgaggcg ctcgaccaga tcgcgctcca
     2461 ggacgccggc cccgttcacg acctgaccgg gccgaagacg atcggcgagt tctgcgaact
     2521 gatcggcagc ctcgacctgt ttccctcccc gccggagcgg gagacctcaa ggctctaccg
     2581 ccgctgggcc ttcgaatcgg cggccctcga cctggccctc tggcaggcgg ggacctcgct
     2641 ggccgaattc cttgcccggc c
//
LOCUS       DAOTIP010000252         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45650_length_2660_cov_4.430710, whole genome shotgun sequence.
ACCESSION   DAOTIP010000252 DAOTIP010000000
VERSION     DAOTIP010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45650_length_2660_cov_4.430710"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="P5138_05630"
     CDS             complement(<1..820)
                     /locus_tag="P5138_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRV60090.1"
                     /translation="MDLALELERATGLKRPVATVLTRRGHGKPADAQAFLAAAESHDP
                     SSFAGMDEAVTLVGEALAAGERITVYGDFDCDGVCATAILVAAIRELGGQCDWFIPDR
                     IADGYGLNQESIRQISERGTKLVITVDCGVTSVEEVALARDLGMKIVVTDHHKVEPDL
                     PDCPILHPQVSGYPFPFLCGAAVAAKFASALRKQAGVAPERDEEDLDLVALATVADVM
                     PLTGENRHLVREGVKVARRARRLGLAALIADARLEPSRLNSEDFGFKLGPRINAA"
     gene            complement(852..1145)
                     /locus_tag="P5138_05635"
     CDS             complement(852..1145)
                     /locus_tag="P5138_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60091.1"
                     /translation="MSIVIWFTVALALWHFTIFVPDRFWQGIIGAFIGCVVGGMISGA
                     IVELILGNGLSDTDLITFLAAVPGTALGAWVIYRIGVSQGTEPIEDIGPTEKV"
     gene            complement(1145..>2660)
                     /gene="secF"
                     /locus_tag="P5138_05640"
     CDS             complement(1145..>2660)
                     /gene="secF"
                     /locus_tag="P5138_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRV60092.1"
                     /translation="LVYAVLFLALIKLIPITMTLPGIAGLVLTIGVAADSNIVIFERV
                     KEEMRAGRSVQSAITLGYRRGISTIIDANVVTLLTAFILFVLATSGIKGFAFTLGVGT
                     LVSLLTAVVFTQALLGSMSNTKLLRSGKGFSGMEGKERWHFDFMGNAPKFFAISGVIL
                     LIGAGALTTKGLNFGIDFESGTRIEVSLDKSASTSEVRDVLSPLGLGNADIQTISNNP
                     ALGDNAFQIESDTLDPSQVSDVDDALDSNFGVSADGFNSTSVGPTFGASVARSAALAI
                     FFSLILIMGYVAIRFGGKYTVPVLIAIAHDFLIALGIYALLGFEVSSATVAAFLTILG
                     YSQYDTIIVFDRIRENEPKMPRATYSQVVNKSMSEVMTRSLVTSFSTVIGVVALLIFG
                     GEVLRSFAIAILVGVASGTYSSIFIASPVLALWKEHEPGFKRRRRQQIESQGYVPAFA
                     SDLEVARKDDANGEAVAGDGEDGSPSDTGEKLSPRERRRRERQAARPDTAGEDT"
BASE COUNT          488 a          829 c          884 g          459 t
ORIGIN      
        1 cggccgcgtt gatcctgggt ccgagcttga atccgaagtc ctcggagttg agccgcgacg
       61 gctccagcct ggcgtcggcg atcagcgccg caagtccgag tcgccgggca cgacgggcga
      121 ccttcacccc ttcccgaacc agatggcggt tctccccggt caggggcatc acatccgcga
      181 ccgtagccag ggcgacgaga tcgaggtctt cctcgtcccg ttccggtgcg actccggcct
      241 gttttctcag cgcagaggcg aacttggccg cgaccgctgc cccgcagagg aatggaaagg
      301 ggtagccgct gacctgcggg tggaggatcg ggcagtccgg caggtccggc tcgaccttgt
      361 ggtggtcggt caccacaatc ttcatgccga gatcccgggc cagggcgacc tcctcgacgg
      421 aagtgacccc gcagtccacg gtgatcacca gttttgtgcc gcgctcgctg atctggcgga
      481 tcgactcctg gttcagcccg taaccgtccg cgatccggtc gggaatgaac cagtcgcact
      541 ggccgcccag ttcgcggatc gcggcgacca ggatcgccgt cgcacagacg ccgtcgcagt
      601 cgaagtcccc gtagaccgtg atcctttccc ctgccgcgag tgcctccccg accagggtga
      661 ccgcttcgtc cattccggca aaagacgacg ggtcgtggct ctcggccgcc gcgagaaagg
      721 cctgggcgtc ggcgggcttg ccgtgtccgc gcctggtcag aaccgttgct acaggccgtt
      781 tcagcccggt ggcgcgctcc agttcgagcg ccaggtccac cgggtagggc ttcgcccgga
      841 aacgaatcgc gctagacctt ttcggtcggt ccgatgtctt cgatcggctc ggtgccctgg
      901 ctgacgccga tccggtagat gacccaggcc ccgagcgccg tgccgggcac agccgcgagg
      961 aaggtgatca gatcagtgtc ggagagcccg ttgccgagta tcagttcgac gatcgcgccg
     1021 gagatcatgc ctccgacgac gcagccgatg aaggccccga tgatgccctg ccagaaacgg
     1081 tcgggcacga agatcgtgaa gtgccagagg gcgagggcga cggtgaacca gataacgatc
     1141 gacatcaggt gtcctctcct gccgtgtccg ggcgggccgc ctggcgttcc cggcggcggc
     1201 gttcccgggg cgaaagcttt tccccggtgt cagaggggct tccgtcctcc ccgtcaccgg
     1261 ccactgcttc gccgttcgcg tcgtccttgc gagcaacttc aagatccgag gcgaaggccg
     1321 ggacataacc ctgagactcg atctgctggc gacgacgccg cttgaatccg ggctcgtgtt
     1381 ccttccacag ggcgagaacc ggcgaggcga tgaagatcga ggagtaggtg ccggaggcga
     1441 caccgaccag gatcgcgatg gcgaacgagc ggagcacctc gccaccgaaa atcagcagtg
     1501 cgacgacacc gatcacggtc gagaagctgg taaccagcga tctcgtcatg acctcgctca
     1561 tcgacttgtt gaccacctgc gagtaggtag cccgcggcat cttcggttcg ttctcgcgga
     1621 tgcggtcgaa cacgatgatc gtgtcgtact gggagtaacc cagaatcgtc aggaaggcgg
     1681 ccaccgtggc gctcgagacc tcgaacccga gcaaggcata gatcccgagt gcgatcagga
     1741 agtcgtgggc gatggcgatc aggaccggga ccgtgtactt gccgccgaag cggattgcca
     1801 cgtaccccat gatcaggatc agcgagaaga agatcgcgag tgcggcgctg cgggcaacag
     1861 aggcgccgaa ggtcgggccg accgatgtgc tgttgaagcc gtcggcgctg accccgaagt
     1921 tactgtccag ggcgtcgtcc acgtccgaga cctgggacgg gtcgagggtg tcgctctcga
     1981 tctggaaagc gttgtcaccg agggccgggt tgttcgagat cgtctggatg tcggcgttgc
     2041 ccaggccgag cggactgagg acgtcacgga cctccgaggt ggaagccgac ttgtcgaggc
     2101 tcacctcgat ccgggtgccg gactcgaagt cgatgccgaa attgagtccc ttggtggtca
     2161 acgcgccggc gccgatcagc aggatcacac ccgatatggc aaagaacttg ggcgcgttgc
     2221 ccatgaagtc gaagtgccat ctctccttgc cttccatccc cgagaatccc ttgccggatc
     2281 tgagcagctt cgtgttcgac atcgaaccga gcagggcctg ggtgaagacg acggcggtga
     2341 gcagcgagac gagggtgccg acaccgagcg tgaaagcgaa gcccttgatg cccgaagtgg
     2401 cgagcacgaa gaggatgaag gcggtgagca gggtgacgac gttggcgtcg atgatcgtgc
     2461 tgattccgcg ccggtaaccg agcgtgatcg ccgattgcac ggatcttcct gcacgcatct
     2521 cttccttgac gcgttcgaat atgacgatgt tcgagtctgc cgcgaccccg atcgtcagca
     2581 ccagacccgc gattcccggc agggtcatgg tgatcgggat cagcttgatc agggcgagga
     2641 agagaaccgc gtagaccagc
//
LOCUS       DAOTIP010000253         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45662_length_2660_cov_2.912476, whole genome shotgun sequence.
ACCESSION   DAOTIP010000253 DAOTIP010000000
VERSION     DAOTIP010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45662_length_2660_cov_2.912476"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="P5138_05645"
     CDS             complement(<1..772)
                     /locus_tag="P5138_05645"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRV60093.1"
                     /translation="MILLTGATGSIGSRLLPRLIEDGHQVRCLVREPRRLGSHRVDVQ
                     ITLGDLRELRDPYKLRQAMRGVDTVIHLAASIRDQPPYRVEELNGLATVRLLEAAERA
                     DVKRFVFFSALNASAVQRTRFFRAKWVAEQAVARSHLDSTIFRPSIVFDHSDPWIALL
                     RRFSFLPAIPISGNGKSRYQPVWADDVARAVEHSLKEDGPGDHLYELAGPATMTYAEM
                     SELVGLLAGRPRPVIRIPLQLVHLGLTAIHRLVGENAFA"
     gene            873..1136
                     /locus_tag="P5138_05650"
     CDS             873..1136
                     /locus_tag="P5138_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRV60094.1"
                     /translation="MSYVIAEPCIGQKDNSCVEVCPVDCIHPTPDEPGYEEAEMLYID
                     PDECIDCDACVEACPVDACFAEDQLPAEWAHFAEKNAQYFQQS"
     gene            complement(1133..2290)
                     /locus_tag="P5138_05655"
     CDS             complement(1133..2290)
                     /locus_tag="P5138_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRV60095.1"
                     /translation="MSLPTEEQVREKLTAVIDPELRRNIVELGMVRSIEIREDGQINV
                     TVSLTTPGCPIRSHFQDAVAQQVSSLEGVTGVGVGFDVLSDDEKAGLRETLGRGKLPE
                     GALAQVKNVICVASGKGGVGKSTMTANLAAALQAEGMKAGALDCDVYGYSIPRMLGVD
                     RKPEVNAERKILPPVADSGVKTMSIGYFVEQNSAVVWRGPMLHKAIQQFLEDVAWEEL
                     DYLLLDLPPGTGDIAMTLAQLLPQAKIAIVTTPQPAAQSVARRSAEMAAKVDLEVMGI
                     IENMSGFTTPSGETFSIFGEGGGKLLADELEVPLLGTIPLSEELREHADVGNPLSVDQ
                     PESPAGKAIVEIARKIIASSPRELPMVQTMQASAPELKPKLGKNARELPLV"
     gene            2366..>2660
                     /locus_tag="P5138_05660"
     CDS             2366..>2660
                     /locus_tag="P5138_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60096.1"
                     /translation="MTPTEEPTMAFRNRFAALAVILLAITFFAISAVAATDGAAAARY
                     VVAQCGWHAGQDASWFDSSADKFGKSNYCQTPESADPFEGVHLIPVNRYRQVAT"
BASE COUNT          445 a          868 c          896 g          451 t
ORIGIN      
        1 ttgcgaaggc gttctccccg accagcctgt ggatcgcggt caggccaaga tggaccagct
       61 gcagcggaat ccggatcacc gggcggggcc tgccggccag aagtccgacc agttcggaca
      121 tctccgcgta ggtcatggtg gccggacccg ccagttcgta aaggtggtcg cccgggccgt
      181 cctccttcag ggaatgctcc accgcccggg ccacgtcatc cgcccagacc ggctggtagc
      241 gggacttgcc gttgcccgag atcgggatcg ccgggaggaa tgagaaacgc cttagcaggg
      301 cgatccacgg gtccgagtgg tcgaagacga tcgaaggacg gaagatggtc gaatcgagat
      361 gggagcgggc gaccgcctgc tccgccaccc acttggcccg gaagaaacgg gtccgctgga
      421 ccgccgaagc gttcagggca ctgaagaaaa cgaagcgctt cacgtcggcc cgctccgctg
      481 cctcgagcag gcggaccgtg gccaggccgt tcagctcctc gacccggtag ggcggctggt
      541 cccggatcga agcggccagg tggatcaccg tgtccacccc ccgcatcgcc tggcgcagct
      601 tgtacggatc ccgcaactca cgcaggtctc ccagcgtgat ctggacgtcg acccgatggg
      661 agccgaggcg gcggggctca cggaccaggc agcggacctg gtggccgtct tcgatcaggc
      721 ggggcagaag acgggagccg atcgagcctg tggcaccggt taggaggatc acctggcgga
      781 gtctatgggg cgttcaccgg cggccgccgg acaggcaaac ccgaccccgg cgaggggaag
      841 gtgccgcacg ctcaacgtct aatatggagt ccatgagtta cgtcatcgca gagccatgca
      901 tcggtcaaaa ggacaactcc tgcgtcgagg tgtgtccggt ggactgcatc cacccgaccc
      961 ccgatgagcc tggttacgaa gaggcggaaa tgctctacat cgatccggac gagtgcatcg
     1021 actgcgacgc ctgcgtcgag gcctgcccgg ttgacgcctg cttcgccgag gaccagctcc
     1081 cggccgaatg ggcgcacttc gccgagaaga acgcccagta cttccagcag tcctagacca
     1141 gcggtagctc tctggcgttc ttgccgagct tcggcttgag ctcgggcgcc gaagcctgca
     1201 tcgtctggac cattggcagt tcgcggggcg aggaagcgat gatcttgcgg gcgatctcga
     1261 cgattgcctt gccggccggg gattcgggct gatcgaccga tagcgggttg ccgacgtcgg
     1321 catgctcgcg cagctcctcc gagagcggaa tggtgccgag cagcggcact tcgagttcgt
     1381 cggccagcag cttgccgccg ccctcgccga agatcgagaa ggtctcgccg gaaggcgtgg
     1441 tgaagccgga catgttctcg atgattccca tcacctcgag atcgactttg gccgccatct
     1501 cggccgagcg cctggctacc gactgggcgg ccggctgcgg cgtcgtgacg atcgcgatct
     1561 tcgcctgggg cagaagctgg gcgagggtca tggcgatgtc gccagtaccg ggcggcaggt
     1621 cgagcagcag gtagtcgagc tcctcccagg ccacgtcctc gaggaactgc tggatcgcct
     1681 tgtggagcat cgggccgcgc cagaccactg cggagttctg ctcgacgaag tagccgatcg
     1741 acatcgtctt gacgcccgag tcggcgaccg gcggcaggat ctttcgctcg gcgttgactt
     1801 ccggcttgcg gtcgactccc agcatgcgcg gaatcgagta gccgtatacg tcgcagtcga
     1861 gggcgccggc cttcattccc tcggcctgaa gggcggcggc aaggttggcg gtcatggtcg
     1921 acttgccgac cccgcctttg ccggaggcga cgcagatcac gttcttgacc tgggccaggg
     1981 ctccctcggg cagcttgccc cggccgagcg tttcgcgcag tcccgccttc tcgtcatcgg
     2041 agaggacgtc gaagccgact ccgactccgg tgaccccctc cagcgaggag acctgctggg
     2101 cgaccgcatc ctggaagtgg gaacgaatcg gacagccggg tgtggtcagc gagacggtca
     2161 cgttgatctg gccgtcttcc ctgatttcga tgctgcggac catgccgagc tcgacgatgt
     2221 tgcggcgaag ctccgggtcg atgaccgcgg tcagcttctc tcttacctgc tcttcggtcg
     2281 ggagggacat ggcctgattc tcgcagagca cggcctctgg ccgccggggg gcgagtttcg
     2341 ggcgcgggcc acttagtccg ggcagatgac cccgaccgag gagcccacca tggcattccg
     2401 gaaccgtttc gccgccctcg cggtgatcct gctcgcaatc accttcttcg cgatctcggc
     2461 ggtggccgcc acagacggcg ccgccgcagc ccgttatgtg gtcgcccagt gtggttggca
     2521 tgccggccag gacgccagct ggttcgactc cagcgccgac aagttcggca agagcaacta
     2581 ctgccagacc cctgagtcgg ccgacccctt cgagggcgtc cacctgatcc cggtcaatcg
     2641 ctaccgccag gtcgccacgc
//
LOCUS       DAOTIP010000254         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45873_length_2650_cov_3.400000, whole genome shotgun sequence.
ACCESSION   DAOTIP010000254 DAOTIP010000000
VERSION     DAOTIP010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45873_length_2650_cov_3.400000"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1772)
                     /gene="ilvD"
                     /locus_tag="P5138_05665"
     CDS             complement(84..1772)
                     /gene="ilvD"
                     /locus_tag="P5138_05665"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV60097.1"
                     /translation="MSVNDASLNLPRPRSAAVFDGPDRAAARAYMKGIGYSEEDLKKP
                     TIGIANTWTDAMPCNYGLRDLAAYVKEGVKAAGGTPMEFNTVAVSDGITMGTQGMKSS
                     LVSREMVADSIELMARGYQFDAIIGLAACDKTIPGAVMGVARIDIPSIVLYGGSVKPG
                     RYKGKDVSILEVFEAVGAHIAGRISDEELTAIEDVASPGYGACGGQFTANTMACAFEA
                     LGISPAFSAMVPAEDEQKTVVAEKIGELILKVLAEDLRPSRIITRRSLENAIAAVCAS
                     GGSTNGVLHLLAIAHEAGVDLDIDVFEEVSRRTPLLADLKPGGRYVATDLYRAGGVPL
                     ILRNLKQGGLLHEDEITVTGRTIGEEVEGAEEEPGQDVVLPVDRPLKAQGGLAILRGN
                     ICPDGAVVKVAGTERRRHVGPARVFEREEDCFAAVKRGEIEKGDVIVIRNEGPSGGPG
                     MREMLQVTAALVGEGMGEHVALLTDGRFSGATRGLMIGHVAPEAVKGGPIGAIHDGDE
                     ITVDIDERRLEVNLGPEQIDERLKGYSAPAPLYDRGVMAKYAATVSSASRGAVT"
     gene            complement(1840..2640)
                     /gene="thiD"
                     /locus_tag="P5138_05670"
     CDS             complement(1840..2640)
                     /gene="thiD"
                     /locus_tag="P5138_05670"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRV60098.1"
                     /translation="MTADRPIPTVLSIAGSDSGGGAGIQADLKAFAACGVHGTTAITA
                     ITAQNTVGVDLIEAVSPEMIVAQIRAVVEDIGVDAVKIGMLGDRPTIKAVAKGLDLLE
                     DAPVVLDPVMVSESGADLLAVEARESLVEMILPRAAVATPNIPEARVITGLTESGAEG
                     PGQAELARAVVGLGGRAVVVTGGHSEQMVDLFFDGEREVEISGEVHPGRSAHGSGCTH
                     SSALAAHLALGLDPLEAAREARKLAARAVADSLEEVGAGPGPVNALGL"
BASE COUNT          402 a          956 c          831 g          461 t
ORIGIN      
        1 gcgagacctg cgcccaatgc cctgctccgg ttcctcaagt gactgcccct ctcctcgctc
       61 cggccggcct ggtggcctgc ctcttacgtg accgcccccc tcgaagcgga actcaccgtc
      121 gcggcgtact tcgccatgac gccacgatca tagagcggag cgggagcgga ataccccttc
      181 agccgttcgt cgatctgttc cgggccgagg ttgacctcga ggcgacgttc gtcgatgtcg
      241 accgtgatct cgtcaccatc atggatcgca ccgatcggcc cgcccttgac cgcttccggg
      301 gcaacgtggc cgatcatcag gccccgggtc gcccctgaga aacgcccgtc ggtgagcagt
      361 gcgacatgct cgcccatgcc ctcgccgacc agggcggcgg tcacctggag catctcgcgc
      421 ataccgggcc ctccggaggg cccttcgttc ctgatcacga tcacgtcgcc cttttcgatc
      481 tcgccccgct tgaccgcggc gaagcagtcc tcctcgcgtt cgaagacgcg ggcaggaccg
      541 acatgcctgc ggcgttcggt tccggcaacc ttgaccactg cgccgtccgg gcagatgttg
      601 ccccgcagaa tggcgagccc cccctgggcc ttcagcggcc ggtcgaccgg caggaccacg
      661 tcctgaccgg gctcctcctc cgcgccttcc acttcctcgc cgatcgtccg gccggtgacc
      721 gtgatctcgt cctcgtggag caggccgccc tgtttcaggt tcctgaggat cagcggtacc
      781 cctccggccc ggtagaggtc ggtcgccaca tagcggccgc cgggcttgag gtcagccagc
      841 agcggggtcc gccgcgagac ttcctcgaag acgtcgatgt caaggtcaac cccggcctcg
      901 tgggcgatcg ccagcaggtg gagcacgccg ttggtcgaac cgcccgaagc gcagaccgcg
      961 gcgatcgcat tctccaggga ccggcgggta atgatccggc tgggccgcag gtcctcggcc
     1021 agaaccttga ggatcagctc gccgatcttc tcggcgacca ccgtcttctg ctcgtcctcg
     1081 gccggaacca tcgccgagaa ggccggggaa atccccagag cctcgaaggc acaggccatc
     1141 gtgttcgcag tgaactgccc gccgcaggcg ccgtacccgg ggctggctac atcctcgatt
     1201 gcggtcaact cttcgtcgct gatcctcccg gcgatgtgag cgccgacggc ttcgaagacc
     1261 tcgaggatgc tgacgtcctt gcccttgtat ctgccgggct tgaccgagcc gccgtaaagc
     1321 acgatcgagg gaatgtcgat tcgggccacg cccatcactg cgccgggaat ggtcttgtcg
     1381 caggcggcca gcccgatgat cgcatcgaac tggtagccgc gggccatcag ttcaatcgag
     1441 tccgccacca tctcgcggct gaccagcgag gacttcatcc cctgcgtccc catggtgatc
     1501 ccgtccgata cggcaacggt gttgaactcc atgggagttc cgccggccgc tttcacccct
     1561 tccttcacgt atgcagcgag gtcgcgcagg ccgtagttgc atggcatcgc gtcggtccag
     1621 gtgttggcga tcccgatggt cggcttcttg aggtcctcct cgctgtagcc gatgcccttc
     1681 atgtaggccc gggcagcggc ccggtcgggt ccgtcgaaga ccgcggccga tcggggacga
     1741 ggcaggttca gggaagcgtc gttcacggac atgcccccat cctaagccga ccgggtcagg
     1801 cgcccgagcc catcgggtca gatgggggcg gcgaccgggt cagaggccca gtgcgttgac
     1861 cgggccgggg ccggctccga cctcttcgag gctgtccgcg accgcccgcg cggcaagctt
     1921 ccgggcctcc ctggccgctt ccagcggatc gagacccaaa gcaaggtgtg ctgccaatgc
     1981 cgaagaatgc gtgcagccgg agccgtgtgc ggatcggccg ggatggactt caccactgat
     2041 ctccacctcc cgctcgccgt cgaagaagag gtcgaccatc tgctcgctgt ggccaccggt
     2101 gaccactacc gccctccctc caagtcccac gaccgcccgg gccagttcag cctgccccgg
     2161 accttctgcc ccggactccg tcaggccggt tatcacccgc gcctcgggga tgttcggtgt
     2221 tgccaccgcc gcccggggaa ggatcatctc gacaagtgat tcgcgggcct cgaccgcgag
     2281 caggtccgcc ccgctttcgg aaaccatcac cggatccagc accaccgggg cgtcttccag
     2341 cagatccagg cccttggcga ccgccttgat cgtggggcgg tcaccaagca tgccgatctt
     2401 caccgcgtcc actccgatgt cctcgaccac cgcccggatc tgggcgacga tcatctccgg
     2461 gctgaccgcc tcgatcaggt cgaccccgac cgtgttctgg gccgtgatcg cggtgatcgc
     2521 cgtggtgccg tggacaccgc aggcggcaaa tgccttcagg tccgcctgga tgccggcgcc
     2581 gccgccggag tcggatccgg cgatcgagag cacggtcggt atgggccggt cagccgtcac
     2641 gggtcgaagg
//
LOCUS       DAOTIP010000255         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45907_length_2649_cov_2.360062, whole genome shotgun sequence.
ACCESSION   DAOTIP010000255 DAOTIP010000000
VERSION     DAOTIP010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45907_length_2649_cov_2.360062"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="P5138_05675"
     CDS             <1..930
                     /locus_tag="P5138_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRV60099.1"
                     /translation="HRALRALFGIAWLLLARWTPPPLHRWRRLVLALFGARLDPTARV
                     YGSVRVWYPPNLTMGRHACLGPGVRCYSMDKVTLGDYATVSQYAHLCAGTHLVDDPDY
                     TLVTRPILIHAILETAQGVTNVEDIAAASPRMQGMSFGPADLAASRRMKTTRVGGGHP
                     GYRSMEDPVADDPEAPRVTAQQDPWHYSIARMVDACTANDILPFYGPFGDIKDLDGCE
                     TQFRSAFLLGCVGAWSLHPAQIAVAKKVFSPPVDEVIFAKKVIEAIPDGRGVHMIDGK
                     MQDDATWKQCKVMVELAEMLAAKDPELKEQYGF"
     gene            complement(931..2229)
                     /locus_tag="P5138_05680"
     CDS             complement(931..2229)
                     /locus_tag="P5138_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60100.1"
                     /translation="MACLTSLRTGLALALVLAGLGSTASSQATAAEPARTVPDVNYNL
                     DELAPPESWQNSLDLYLPAIPDGAGSELRPVVIYVHGGGLMIGDKSNRMPDKVSLFNG
                     LGYIFASVNYRLSPDITDGDLSDAFAPGRVRAPDHLADVAEAVAWISRNIRRYGGDPD
                     RLILIGHSSGGQMVNLVGTNPAWVRGRWMSPAQILGVVSLGSDTFDVRSEADPATSTA
                     SFSRRTSFWQVFGTPAEEAADPRWDSQSPLLSADPSDPPFLFITQSARPARIASSSEM
                     ASKLGQDPDTSVVPVPYDHDGINTALGSAGDSSQETARVSQFMEQLVNSAGSAGVRIT
                     RRPAGRVVVKVKRRATRKATKKAMRNVRRKVAFRFEGKGRARGLQCRIDGAKFSRCRS
                     PKSYRLKPGKHTFRVRALYPSGRPGDERKLTFRIVARVRR"
     gene            complement(2267..>2649)
                     /locus_tag="P5138_05685"
     CDS             complement(2267..>2649)
                     /locus_tag="P5138_05685"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRV60101.1"
                     /translation="PLFRKGMYKTLLMLRKTGAKVTLMRDLPMSKTFLPSVCVSENVN
                     DPGRCSFPAVRPLSEAYDFAAAKRLKAVQIIDPLPKVCPGSRKCRAVHGNVLKYRDRG
                     HISATFCRLLTNWLDRRLQDPFRG"
BASE COUNT          432 a          891 c          888 g          438 t
ORIGIN      
        1 caccgggcgc tcagggcgct gttcggcatc gcctggttgc ttctcgcgcg ctggaccccg
       61 ccgccgctgc accgctggcg ccggctggtt ctggcactgt tcggcgcccg gctcgatccc
      121 acggcccgcg tctatggcag tgtccgggtc tggtatccgc caaacctcac catgggccgt
      181 cacgcttgcc tcggccccgg cgtgcgctgc tattcgatgg ataaggtgac gctgggcgac
      241 tacgcgacgg tgtcgcaata cgcccatctc tgcgccggaa cccatctggt cgacgatcct
      301 gactatacgc tggtcacccg gccgatcctg atccacgcga tcctcgagac cgcccagggc
      361 gtgaccaacg tcgaggacat cgccgcggcc agcccccgca tgcagggcat gagcttcggc
      421 ccggccgacc tggccgcctc gcgccgcatg aagaccaccc gcgtcggcgg cggccacccc
      481 ggctaccgct cgatggagga cccggtcgcc gatgacccgg aggccccgcg tgtcaccgcc
      541 cagcaggacc cgtggcatta ctcgatcgcc cgcatggtcg atgcctgcac cgccaacgac
      601 atcctgccct tctacggccc cttcggcgac atcaaggacc tcgacggctg cgagacccag
      661 ttccgctccg ctttcctgct cggctgcgtc ggcgcctggt cgctccaccc cgcccagatc
      721 gcggttgcca agaaggtctt ctcgccgccg gtcgacgagg tcatcttcgc caagaaggtg
      781 atcgaggcga tcccggatgg ccgtggcgtc cacatgatcg acggcaagat gcaggacgac
      841 gccacctgga agcagtgcaa ggtgatggtc gaactggccg agatgctcgc ggccaaggac
      901 cccgagctca aggagcagta cggcttctag ctagcgacgg acacgggcga cgatgcggaa
      961 ggtgagcttc cgttcgtcgc ccggtcgtcc gctcgggtaa agcgcccgga cccggaacgt
     1021 gtgtttgccg ggcttcaacc ggtagctctt cgggctccgg cagcggctga acttcgcccc
     1081 gtcgatccgg cactgaaggc cgcgggcccg tcccttgccc tcgaacctga aggcgacctt
     1141 gcgacgcacg ttccgcatcg ccttcttcgt cgccttccgg gtcgcccggc gcttcacctt
     1201 cacgaccacc cggccggcgg gccggcgggt gatccttact cctgccgagc cggccgaatt
     1261 gaccagctgt tccatgaact ggctgacccg cgcggtctcc tgcgaggagt ccccggcgga
     1321 accgagggca gtgttgatcc cgtcgtggtc gtagggaacc gggaccaccg aggtgtccgg
     1381 gtcctggccg agtttcgacg ccatttcgct gctggaggcg atccgggccg gccgggccga
     1441 ctgggtgatg aaaaggaacg gcggatccga cgggtcggca gacaggagcg gagactggga
     1501 atcccagcgc ggatcggccg cttcctcggc cggcgtaccg aagacctgcc agaacgaagt
     1561 cctccgtgag aagctggccg tggaggtggc ggggtcggcc tccgagcgga cgtcgaaggt
     1621 gtcggagcca agtgagacga cacccaggat ctgggcgggt gacatccagc gacccctgac
     1681 ccaggccgga ttggtgccga ccaggttgac catctggccg ccggaggagt ggccgatcag
     1741 gatcagacgg tcagggtccc ctccgtaacg gcggatgttc cgcgatatcc aggcgaccgc
     1801 ctcggcgaca tcggccaggt ggtcgggcgc ccgcacccgg cccggcgcga aagcgtcact
     1861 caggtcgccg tcggtgatgt ccggcgatag ccggtaattg acgctggcga agatgtagcc
     1921 gagaccgttg aagaggctga ccttgtccgg cattcgattc gacttgtcgc cgatcatcag
     1981 gccgccgcca tgaacgtaaa tgacgaccgg cctgagttcg ctgcctgccc cgtccgggat
     2041 ggcgggaagg tagagatcga ggctgttctg ccacgactcg gggggcgcca gctcgtccag
     2101 gttgtagttc acgtcaggga ccgtgcgggc gggctccgcg gcggtcgcct gcgagctagc
     2161 tgttgacccg aggccggcaa gaaccagcgc gagggcgagg ccggtgcgaa gcgaagtcag
     2221 gcacgccatg tctctggaac cccctttccg gcccagggtc gcggaatcag ccccggaacg
     2281 gatcctggag ccgacggtcg agccagttgg tcaggaggcg gcagaaggtg gcgctgatgt
     2341 gaccacggtc gcggtacttg agcacgttgc cgtgaactgc acggcatttc ctgctgccgg
     2401 gacatacctt cggcagcggg tcgattatct gcactgcctt cagccgcttg gcggcggcga
     2461 agtcataagc ctccgatagc ggccgcacag ccgggaagga gcagcggccc ggatcgttga
     2521 cgttctcgga aacacagacc gaaggcagga aggtcttcga catcggcagg tccctcatca
     2581 gggtgacctt tgccccggtc ttgcgcagca tcagcagggt cttgtacatg cccttccgga
     2641 acagcggct
//
LOCUS       DAOTIP010000256         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45915_length_2648_cov_4.198226, whole genome shotgun sequence.
ACCESSION   DAOTIP010000256 DAOTIP010000000
VERSION     DAOTIP010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45915_length_2648_cov_4.198226"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /gene="nusG"
                     /locus_tag="P5138_05690"
     CDS             <1..475
                     /gene="nusG"
                     /locus_tag="P5138_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRV60102.1"
                     /translation="VKQKLEHRIESMGQGHRVRKVVVPTEQVSEVKDGQKIQVEKRTM
                     PGYVLINMEMTDNAWSLVSNTQGVTGFVGPRDTPVPLTKAEIDRLLHRETAERPRTQA
                     QFEIGETVKIISGPLSDFSGEIAEINDDQQKLKVLVSIFGRETPSEVGYEQVKKL"
     gene            503..925
                     /gene="rplK"
                     /locus_tag="P5138_05695"
     CDS             503..925
                     /gene="rplK"
                     /locus_tag="P5138_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRV60103.1"
                     /translation="MAKKVMTLIKLQIPAGQANPAPPVGPALGQHGVNIMDFCKAFNA
                     QTQQEGGTIIPVEITVYEDRSFDFITKTPPAAVLIKEAIGLQKGSGEPHINKVGTITE
                     AQVRQIAERKMPDLNANDLDAASKIIAGTARSMGVEVK"
     gene            928..1704
                     /gene="rplA"
                     /locus_tag="P5138_05700"
     CDS             928..1704
                     /gene="rplA"
                     /locus_tag="P5138_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRV60104.1"
                     /translation="MAHGKRFRQQFQKIQREHVYPPVEAINLIKETASANFDETVELH
                     VRLGVNVRHAEEQLRGTLALPNGLGKDVKVAVFAQGDKARDAEAAGADIVGADDLAAK
                     IEEGFDDFDVVIATPDMMPTVGRLGRVLGPSGKMPNPKVGTVTEDVEKAVGESKAGKV
                     EYRTDRQAIVHLAIGKASFDAEKLLENYAAVLDEITKAKPASAKGRYILSVSVSTTMG
                     PGISVDPGKATEQDIFPEQGGTAEGETGGEADAEAEAVPA"
     gene            1704..2183
                     /locus_tag="P5138_05705"
     CDS             1704..2183
                     /locus_tag="P5138_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60105.1"
                     /translation="MRFGASAFRGLTLTALCAASIGLAACGSSDDSDQDASSQAADQK
                     AVMAVADQLREAYQQKDPASICDLVDPQGLKEQFVNMKGCEHQIGQAINQAGSSITAA
                     DFNVDTVTVEDDRAVGTQTEGNGNRVFFKKIGGQWYIDVNPTGESESPSAGGAASTQ"
     gene            complement(2193..>2648)
                     /locus_tag="P5138_05710"
     CDS             complement(2193..>2648)
                     /locus_tag="P5138_05710"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60106.1"
                     /translation="IEPPTTSTPFGLHGTLFGISGKGWSILLAHPERNPVFQEDIDLL
                     VSLVDGGIKTQVTAGAFTGQYGRTAARTAARMMEMGLVHTVASDAHHATLRPPEMAGP
                     LTEAGYGHMVDYLCSEMPAWILDGGPEPRPPENTKAPKKGLLGRFRNRG"
BASE COUNT          539 a          838 c          835 g          436 t
ORIGIN      
        1 ggtcaagcag aagcttgagc atcgcatcga gtcgatgggg cagggtcacc gtgtccgcaa
       61 ggtggtagtc ccgaccgaac aggtgtccga agtcaaggac ggccagaaga tccaggtcga
      121 gaaacgcacc atgcccggct atgtcctgat caacatggaa atgaccgaca acgcgtggag
      181 cctggtctcc aacacccagg gcgtcactgg tttcgtcggc ccccgtgaca ccccggtgcc
      241 gctgaccaag gcggaaatcg accgtctgct ccaccgggag accgcggagc gtccccgcac
      301 ccaggcccag ttcgagattg gcgagaccgt caagatcatc agcggtccgc tttccgactt
      361 ctccggcgag atcgccgaga tcaacgacga ccagcagaag ctcaaggtgc tcgtctccat
      421 tttcggtcgt gagactccgt ccgaagtcgg ttacgaacag gtcaagaagc tctaacccac
      481 aactcttaag gcatcaagtc ccatggctaa gaaagtaatg accctcatca agctccagat
      541 tccagcaggc caagccaacc cggcgccgcc ggtcggccct gcgctcggcc agcacggggt
      601 caacatcatg gacttctgca aggcgttcaa cgcacagacg cagcaagagg gtggcacgat
      661 cattccggtc gagatcacgg tctacgaaga ccgttccttc gacttcatca ccaagacgcc
      721 cccggcggcc gtgctgatca aggaagcgat cggacttcag aagggctcgg gagagcctca
      781 catcaacaag gtcggcacca tcactgaggc ccaggtgcgg cagatcgcgg agcgcaagat
      841 gcccgatctc aacgcaaatg acctggacgc cgcctcgaag atcatcgccg gcaccgcccg
      901 ttctatgggc gtggaggtca agtaatcatg gctcacggca agcgcttccg ccagcagttc
      961 cagaagatcc agcgggaaca tgtgtacccg cccgtcgagg cgatcaacct gatcaaggag
     1021 acggcttcgg ccaatttcga cgagaccgta gaactccacg tgcgtctcgg ggtcaacgtc
     1081 cgtcacgccg aagagcagct ccgtggcacc ctggcactgc ccaacgggct cggcaaggat
     1141 gtcaaggttg cggtcttcgc tcagggtgac aaggcccgtg acgccgaggc cgccggagcc
     1201 gacatcgtcg gtgccgatga cctcgccgcc aagatcgaag aaggcttcga cgatttcgac
     1261 gtggtcattg ccaccccgga catgatgccg accgtcggcc ggctcggacg ggtcctcggg
     1321 ccctcgggca agatgccgaa ccccaaggtc ggcacggtca ccgaggatgt cgaaaaggca
     1381 gtcggcgagt ccaaggccgg caaggtcgag taccgcactg accggcaggc gatcgttcac
     1441 ctggcgatcg gcaaggcctc gtttgacgcc gaaaagctgc ttgagaatta tgccgccgtg
     1501 ctggacgaga tcaccaaagc caagcccgcc tcggccaagg gccgttacat cctttcggtt
     1561 tcggtctcga ccaccatggg tccgggcatc tccgtagatc cgggcaaggc gaccgaacag
     1621 gacatcttcc ccgagcaggg tggcacggct gaaggcgaaa ccggcggcga agccgacgcg
     1681 gaagctgagg ccgtaccggc ctgatgcgct tcggcgcgtc cgcgtttcga ggtctgacgc
     1741 tgacggcgct ttgtgccgcc tcgatcgggc tggctgcctg cggcagctcg gacgattccg
     1801 atcaggatgc gtccagccag gccgccgatc agaaggccgt gatggcggtt gccgatcagc
     1861 tgcgggaggc ttaccagcag aaggatccgg cctcgatctg cgacctggtt gatccgcagg
     1921 gcctgaagga gcagttcgta aacatgaagg gctgcgagca ccagatcggc caggcgataa
     1981 accaggccgg cagctcgatc actgccgcgg atttcaacgt ggacacggtc acggtcgaag
     2041 atgaccgggc ggtgggaacc cagaccgaag gtaacggcaa ccgcgtcttc ttcaagaaga
     2101 tcggcggcca gtggtacatc gacgtaaatc ccaccggcga atccgaatcg ccgtctgccg
     2161 gtggcgccgc cagcacgcag taacgggtcc gctcagcccc ggttccggaa ccgccccaga
     2221 agccccttct tcggggcctt ggtgttttcc ggcggtctcg gttccgggcc gccgtcgagg
     2281 atccaggccg gcatttccga gcagagataa tcgaccatgt gtccgtaacc ggcttcggtc
     2341 aggggcccgg ccatctccgg tggccgcagg gttgcgtggt gcgcgtcgga agcgacggtg
     2401 tggaccaggc ccatctccat catcctggcg gcggtacggg cggccgtccg accgtactgc
     2461 ccggtgaaag ctccggcggt cacctgggtt ttgatcccac cgtcaaccag tgagacgagc
     2521 aggtcgatgt cctcctggaa aaccgggttc ctttccgggt gggcgagcag gatgctccag
     2581 cccttgccgc ttattccgaa gagcgtgccg tgcaatccga agggggtcga ggtggtcggc
     2641 ggctcgat
//
LOCUS       DAOTIP010000257         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_45964_length_2646_cov_3.336550, whole genome shotgun sequence.
ACCESSION   DAOTIP010000257 DAOTIP010000000
VERSION     DAOTIP010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45964_length_2646_cov_3.336550"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="P5138_05715"
     CDS             <1..677
                     /locus_tag="P5138_05715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRV60107.1"
                     /translation="RCFDPNRLCNRSHHRIRLFWAMHVVHHSSEHYNFSTALRQDWSP
                     FTSIFFWMPGALLFEPWMIYLAFSWSLLYQFLLHTEAVRKLPRPIEFIFNTPSHHRVH
                     HGSQEQYLDKNYGGILIIWDRMFGSFEPEEERVRYGLTRNIETFHPVKVAYGEYVNIW
                     RDVRAADNWRDRLGFTFKGPGWKPRESAGGVSPEAADEVGVDQADRLHQGVHVRRADE
                     LESTRA"
     gene            complement(561..1136)
                     /locus_tag="P5138_05720"
     CDS             complement(561..1136)
                     /locus_tag="P5138_05720"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRV60108.1"
                     /translation="MSELEDDGRIRCGWANGSEMMRDYHDEEWGIPVAGEQAMFERLS
                     LEAFQAGLSWATILRKRPAFREAFRDFDLEYCAGLSDRDVENLMGNEGIIRARAKIEA
                     TRSNAAAAIDLRGDGGLEGLISGFAPARSPEPASHSEVPTSSAESEALAKELRSRGFK
                     FVGPTNMYALMEAVGLVNTHLVGCFRRNAAG"
     gene            1167..1919
                     /locus_tag="P5138_05725"
     CDS             1167..1919
                     /locus_tag="P5138_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRV60109.1"
                     /translation="MRRIRKAALLTSFVALAFASGLAPASPAAPAGLKKKVIGRSVEN
                     RPIISWRQGDFAAGFRVLVVGSIHGDERQGMRVVANLRQRLAGRNGPEGIAFWTIRTA
                     NPDGTARNTRRNARLVDLNRNFPYRWDGSLNGGYNSGPRPASEPETRTIMRFSRRVGF
                     DLAIWFHQPWGRTLVPCNRDRRWAILYSRLSGLGTGGGCDRGSYPGSAISWQHHEYGT
                     TAFVVELGPRVQKRAVIARHAGAVIRLARRMS"
     gene            2037..2384
                     /locus_tag="P5138_05730"
     CDS             2037..2384
                     /locus_tag="P5138_05730"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV60110.1"
                     /translation="MVEDSEKVDRVFHALSNGTRRGMVRQLSRGECTVGDLARPADMS
                     FAAASKHVKVLERAGMVNRRVKGRQHICSLRPDPLRDASEWLGFYEKFWSDNLERLAA
                     AVETDYAREQEGR"
     gene            2381..>2646
                     /locus_tag="P5138_05735"
     CDS             2381..>2646
                     /locus_tag="P5138_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRV60111.1"
                     /translation="MTTSGDSVQIVKQVAAPVDRVYQAFIEPEQLVSWMHPDGFTGHS
                     ATNDPREGGRGELKHNLPDGGIGGFNWVYLEVTENERLVLDWQFG"
BASE COUNT          459 a          874 c          849 g          464 t
ORIGIN      
        1 gacgatgttt cgatcccaac cgcctgtgca atcgcagcca ccaccgcatc cgcctcttct
       61 gggcgatgca tgtggttcac cactcgtccg agcactacaa cttctcgacc gcgctccggc
      121 aggactggtc gcccttcacc tcgatcttct tctggatgcc cggcgcgctc ctgttcgagc
      181 cctggatgat ctacctcgcc ttctcgtgga gcctgctcta ccagttcctg ctccacaccg
      241 aggcggtccg gaagctgccg cggccgatcg agttcatctt caacacgccc agccaccacc
      301 gggtccatca cggctcccag gagcagtacc tggacaagaa ctacggcggc atcctgatca
      361 tctgggatcg catgttcggg tccttcgaac cggaggaaga gcgggttcgc tacggcctga
      421 ccaggaacat cgagaccttc catccggtca aggtcgccta cggcgaatac gtgaacatct
      481 ggcgggacgt acgggcggcc gacaactggc gggaccgcct cggcttcacc ttcaaggggc
      541 ccggctggaa gccccgcgaa tcagccggcg gcgtttctcc ggaagcagcc gacgaggtgg
      601 gtgttgacca ggccgaccgc ctccatcagg gcgtacatgt tcgtcgggcc gacgaacttg
      661 aatccacgcg agcgtagttc cttcgccagg gcctccgact cggcggagct ggtcggcacc
      721 tcggagtgac ttgccggctc gggcgagcgg gctggcgcga agccggagat gagtccttcg
      781 aggccgccat ccccgcgcag atcgatcgcc gccgcagcgt tgcttcgggt cgcctcgatc
      841 ttggcgcggg cccggatgat cccctcgttg cccatcagat tctcgacatc ccggtcactc
      901 aggccggcgc agtattcgag gtcgaaatcc cggaacgctt cccggaaggc gggccgcttg
      961 cggaggatgg tcgcccagct gaggccggcc tggaaggctt ccagggagag gcgttcgaac
     1021 atcgcctgct cgccggccac cggaattccc cattcctcgt cgtgatagtc ccgcatcatc
     1081 tcgctgccat tggcccagcc gcagcggatc cggccgtcat cttcgagttc actcaccggc
     1141 tccatcttgc cagctaggtt gaacgggtga gaaggatccg gaaagccgct ctgctgacat
     1201 ctttcgtcgc cctggcattt gcctcgggtc tggcgccggc gtcgccggcc gccccggccg
     1261 ggctgaagaa gaaggtgatc gggcgctccg tcgagaaccg tccgatcatc agctggcggc
     1321 agggtgattt cgcggccggt ttccgggtgc tggtggtcgg ctcgatccac ggggacgagc
     1381 gacagggcat gagggtcgtg gccaacctgc gccagcggct ggcagggcgg aatggaccgg
     1441 aggggatcgc cttctggacg atcaggaccg ccaacccgga cggcaccgca aggaataccc
     1501 gccgcaacgc ccggctggtc gatctgaacc ggaacttccc ctaccgctgg gacggcagcc
     1561 tgaacggtgg ctacaactcg ggtccgcggc ccgcctcgga accggagacc aggaccatca
     1621 tgcgcttctc acgaagggtc ggtttcgacc tggcaatctg gtttcaccag ccctggggac
     1681 gcacccttgt cccctgtaac cgggaccgcc gttgggcgat tctctattcc cggctctccg
     1741 gcctcggcac cggcggcgga tgcgaccgcg gcagctaccc gggcagcgcg atctcgtggc
     1801 agcaccatga atacggcacc acggcgttcg tggtcgaact cggcccgcgg gtccagaaga
     1861 gggccgtgat tgcgcggcat gccggcgcgg tgatccgcct ggcccgccga atgagctgag
     1921 tgtcaggaat ccagtcctgc ggcgagaccg cggaagatcc cgttggctca gcttcgtgtc
     1981 cggatcattc aaccaattgg ttgaaaatct ggcgggaatg ttctatcttc aaccacatgg
     2041 ttgaagattc ggagaaggtc gatcgcgtct ttcatgccct ttcgaacggc acccgccggg
     2101 gcatggtccg gcagctctcg cgcggtgaat gcacggtcgg cgacctggcc cgacctgccg
     2161 atatgtcctt cgccgccgcc tccaagcacg tcaaagtgct ggagcgggcg ggcatggtca
     2221 accgccgggt gaaggggcgg cagcacatct gttcgctgag gcccgatcca ctcagggatg
     2281 cgagcgagtg gctcggcttc tacgaaaagt tctggagcga caacctcgag agactcgcag
     2341 ccgcggttga aaccgactac gcccgggaac aggaggggcg atgaccacct ccggcgactc
     2401 ggtacagatc gtcaagcagg tcgcggcccc ggtcgatcgg gtctaccagg ccttcatcga
     2461 accggagcag ctggtcagct ggatgcaccc ggacggcttc accggacact cggcgaccaa
     2521 cgaccctcgt gagggcggca ggggcgagtt gaagcacaac ctccccgacg gaggcatcgg
     2581 gggcttcaac tgggtgtacc tcgaagtcac cgagaacgaa aggctggtcc tggactggca
     2641 gttcgg
//
LOCUS       DAOTIP010000258         2643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46048_length_2643_cov_2.724498, whole genome shotgun sequence.
ACCESSION   DAOTIP010000258 DAOTIP010000000
VERSION     DAOTIP010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46048_length_2643_cov_2.724498"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="P5138_05740"
     CDS             <1..297
                     /locus_tag="P5138_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HRV60112.1"
                     /translation="FYVETLGLRPDPNSEYEFWVGDTCFGIWEPVQQGMEFSAEKCSH
                     LALHVEDMEAARAELESRGVTFFGDTFDTGVCLMALFADPDGNSLMLHHRYKPR"
     gene            666..1448
                     /locus_tag="P5138_05745"
     CDS             666..1448
                     /locus_tag="P5138_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HRV60113.1"
                     /translation="MESKVQHRVDSTSNIIGLADEADLWERRDSDPHAREELVNRYLA
                     FAKSVAVRYRSQAESLDDLVQVASLGLVNAINRFDPARGVPFIAFASPTITGELKRHF
                     RDRTSAMRLPRSLYDRIGQIDIVVSDLTSRLNREPSIAEIAAEMSCPESEVTEAFEAG
                     HNRHPVSLDQSPGSGDEDQSSPAEWLGREDSHYETAERRIMLRDAMDDLSGEERMVIR
                     LRFRDEMTQSEIAEKIGHSQMHVSRMLRRILDRMGDRLPAAA"
     gene            complement(1554..>2643)
                     /locus_tag="P5138_05750"
     CDS             complement(1554..>2643)
                     /locus_tag="P5138_05750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV60114.1"
                     /translation="VAVIGAGIVGLATARALERRGFTCLLFEQGAPGGGQSAGESRIF
                     RHAHNDPRQFELAVRARRDWKEWEAEFGVQLISPDGAMAIGAESVSRLEKYADDPAVR
                     VREMTPEQVTRALPILAGYDGPALIDDAAGAILTRLTIAHLIESVGPNLVPERVESVV
                     PRPEGGVEVTSSFGSRDFDAVVVAAGTGTPALAASADINIPSEPRAHVRLTFPLASGV
                     REPRLACIQDSSGAFGEAAAYGSPVRGNRSYAVGLALSAEVDSESPEDPLSGLAERTR
                     AYVREAMPGLDPDGAVPFQRWITELPWAADGLAIWQEGDAYFVAGHNLFKLAPALGRT
                     LADSVVSGMVEPGFRPEARLGAPALEAR"
BASE COUNT          482 a          906 c          804 g          451 t
ORIGIN      
        1 ttctacgtgg aaaccctcgg cctcaggccc gacccgaact cggaatacga gttctgggtc
       61 ggcgatacct gcttcggcat ctgggagcca gtccagcagg gaatggagtt ctcggccgag
      121 aagtgttccc atctggccct ccatgtggag gacatggagg cggccagggc cgagctcgag
      181 tccaggggcg tcaccttctt cggcgacacc ttcgacaccg gggtatgcct catggcgctc
      241 tttgcagatc ccgacgggaa ttcgctgatg cttcaccatc gctacaaacc gcgctgacgc
      301 ggcccgtcga atcaccttcg cgcgcaggaa cgcccttacg ggaccggccg aaaggcaccc
      361 gggacggttt tttcgttcca aaccggggcc tgacgcaaca cctgcgtact gtgttgtctt
      421 ttttgtgaaa ctgcttgttt cccttctgtt gcgccaaata ctggtgtcaa gaagccagta
      481 ttgaagccat tttctgaacg gcattcggaa acttaaggct ttctttaggt tttggatact
      541 ggacagttca ccgatccttg cttacggttt ccccgaacga tcaggcacca ctgcacggct
      601 ctcggatcga gagcaccagg gcagcaactc aggaggggca ctcctccgga aagagaggct
      661 gaacgatgga aagcaaggtt caacacaggg tggattcgac aagcaacatc atcggactgg
      721 ccgacgaggc cgatctctgg gagcgccgcg acagcgaccc ccatgcccgc gaggagctgg
      781 tcaaccgcta cctcgctttc gccaagagcg tcgccgtccg ctaccgcagc caggcggaat
      841 ccctcgacga cctggtccag gttgcgagcc tcggactggt caacgcgatc aatcgtttcg
      901 accccgcacg tggcgtcccg ttcatcgcct tcgcttcccc caccatcacc ggcgaactga
      961 agcggcactt ccgcgaccgc acctcggcga tgcgactgcc ccgaagcctc tatgaccgga
     1021 tcgggcagat cgacatcgtc gtctcggacc tgaccagccg tctcaaccgc gaaccgtcga
     1081 tcgccgagat cgccgccgag atgagctgcc cggagtccga ggtcaccgaa gccttcgaag
     1141 ccggccacaa ccgtcacccg gtctccctcg accagtcacc cgggtccggt gacgaagacc
     1201 agagctctcc tgccgaatgg ctcggacgtg aggattcaca ctacgagacc gccgagcgcc
     1261 ggatcatgct tcgcgacgca atggatgatc tgagcggaga ggaacgcatg gtgatccgcc
     1321 tccggttccg cgacgagatg acccagtcag agatcgccga gaagatcggc cactcccaga
     1381 tgcatgtgtc acgcatgctg cggcgcatcc tcgaccgcat gggcgatcgc ctccctgccg
     1441 ccgcctgaac ttccctccct ccccaatgac acgaaccgac cggcccgccc cctggccggt
     1501 cggttcgttt aaccctccgg gtcaacgcgg gtcggttcgt ttaaccctcc gggtcaacgc
     1561 gcttcgagag ccggcgctcc aaggcgagct tccggacgga agcccggttc caccatgccg
     1621 gagaccaccg agtcggccag ggtccggccg agtgcgggcg caagtttgaa gaggttgtga
     1681 ccggcgacga agtaggcatc gccttcctgc cagatcgcga ggccgtcggc cgcccacggc
     1741 aactcggtga tccagcgctg gaacgggacg gcgccgtccg ggtccagtcc cggcatggcc
     1801 tcgcgcacat aggcgcgggt ccgctctgcc agcccggaaa ggggatcttc cggcgattcc
     1861 gagtcgacct cggcgctcag ggcgagtccg accgcgtatg aacggttgcc gcggaccggc
     1921 gatccgtagg cggcggcctc cccgaaggct ccggatgaat cctgaatgca ggcgagccgc
     1981 ggctccctga ccccggaagc cagcggaaag gtcagcctga catgggcccg gggttcggac
     2041 ggaatattga tatccgcgct ggcagccagg gccggtgtgc cggtgcccgc ggcgaccaca
     2101 accgcgtcga agtcccggga cccgaaactg ctggtcacct ccacccctcc ttccggacgg
     2161 ggaacgacgc tctcgacccg ctccggcacg agattgggcc cgacagactc gatcaggtgg
     2221 gcaatcgtca gccttgtgag gattgcccct gccgcgtcat cgatcagggc cgggccgtcg
     2281 tagccggcca gtatcggtaa tgcccgggtg acctgttccg gagtcatctc ccgaacccgg
     2341 acggccggat cgtcggcgta cttctcgagt ctcgaaacgg actccgcgcc gatcgccatc
     2401 gcgccgtcgg gggaaatcag ctgtaccccg aactccgcct cccattcctt ccagtcccgc
     2461 cgggcacgga cggcgagctc gaactgcctg gggtcgttgt gggcatgccg gaaaatccgg
     2521 gactctcccg ccgactggcc cccgccggga gctccctgct cgaaaagcag gcaggtaaag
     2581 ccgcggcgct ccagcgcccg ggctgttgcg aggccgacta tccccgcgcc gattaccgcg
     2641 acc
//
LOCUS       DAOTIP010000259         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46157_length_2638_cov_2.782811, whole genome shotgun sequence.
ACCESSION   DAOTIP010000259 DAOTIP010000000
VERSION     DAOTIP010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46157_length_2638_cov_2.782811"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="P5138_05755"
     CDS             complement(<1..281)
                     /locus_tag="P5138_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60115.1"
                     /translation="MPDSPEKKPAGKSGRIPTLAIATVVVGLLASLAIYGLSFVPGTG
                     SLEIGWDTTEPIASPPAKDVGTGSFGVTRTSLSALAPNDDGLLLYRVAGV"
     gene            359..1852
                     /locus_tag="P5138_05760"
     CDS             359..1852
                     /locus_tag="P5138_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRV60116.1"
                     /translation="MSKTARMAHSPRMGLPDRFLRDQALASRISGGDRSAFDEMFRRY
                     HQQIYRFCSAMVGPEEGKDMLQNVMTKAMTSMPHDEDFQMKPWLYRVARNECVDHIRS
                     ARRTDSGLEADGQVDDSSRADPHQSAVDREQLNQLVNDLNGLPEKQRATLVMRELSGL
                     SYQEIADSLGSSEAGAKQIVYEARLSLEQAELGRKLACADVRELISARDRRKLRGRQI
                     RSHMRHCQGCTDFEKAISSRETSFQALSPVLPLAAAAGILGAVRDGGTAVGAVGGGAA
                     AAASGGVVSGGVATGLVTKGVVALVVAGGIGVGTAEVARHHRDASTGQAVTRESSQTL
                     GLGTSAGFGPKSPEATTGDRAFGEHGREGGKREGHGDEGRSGSGRERGGSEKNGQESS
                     GHEAGNDSSGERVTPGQGQGNGPAELPEASSKGQARADEASSAVPGKTGSPPPSQKPA
                     TPPGTSSGGSSGNSQGNSGSAATESSGGNSESAPAVGKPESPGKSGN"
     gene            1930..2289
                     /locus_tag="P5138_05765"
     CDS             1930..2289
                     /locus_tag="P5138_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60117.1"
                     /translation="MKKFALTIVATAALLAPVASATAGTHPEHPDKAKGPKAGKVSKA
                     KAYGKYCKGQSKKHVKGQKGTDFSRCVNAMAKANREDSMSARKACKALSKKHVKGEKG
                     TPFSRCVVGVAQMRKDA"
     gene            complement(2351..2424)
                     /locus_tag="P5138_05770"
     tRNA            complement(2351..2424)
                     /locus_tag="P5138_05770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Arg,seq:ccg)
BASE COUNT          531 a          802 c          900 g          405 t
ORIGIN      
        1 actcccgcga cccggtagag aagcagcccg tcgtcattgg gtgccagggc cgaaaggctg
       61 gtgcgggtga cgccgaaaga cccggtgccc acgtccttcg ccggcggcga ggcgatcggt
      121 tcggtcgtgt cccagccgat ctcgagcgac ccggtgccgg gaacgaagga aagaccgtag
      181 atggcaaggg aagcgagcag gcccacgaca accgtggcga tcgccagggt cggaatcctg
      241 ccgctcttgc cggcgggttt cttctcgggt gaatcgggca ttggaatggc agattattgg
      301 aaaggccctt actttccctg taccggatcg tttcatctgg aaaagggttc tgaagacgat
      361 gtccaagaca gcccgaatgg ctcattcgcc ccgaatgggc ctgcctgacc gcttcttgcg
      421 tgatcaggct ctggcaagcc ggatctccgg aggagaccgt tcggccttcg acgagatgtt
      481 ccggcgctac caccagcaga tctaccggtt ctgctccgcc atggttgggc ccgaagaggg
      541 caaggacatg cttcagaacg tgatgaccaa ggcgatgacg tccatgcccc acgatgaaga
      601 tttccagatg aaaccgtggc tctaccgtgt agccaggaac gaatgcgtcg accacatccg
      661 ctcggcccgg cgtaccgatt ccgggctgga ggccgacggt caggtcgatg attctagccg
      721 ggccgacccc catcagagtg cggttgaccg cgagcagctg aaccagctgg tgaacgacct
      781 caacggactc cccgagaagc agcgggcaac cctggtcatg cgggaacttt ccggcctcag
      841 ctaccaggag atcgcagatt cgctcggatc ctccgaagcc ggtgcgaagc agatcgtcta
      901 cgaggcccgc ctctccctcg agcaggcgga actcggccgg aagctggcct gcgccgacgt
      961 gagagaactg atctcggcac gggatcgccg gaagctccgg ggacgtcaga tcaggtccca
     1021 catgcggcat tgtcagggct gcacagattt tgagaaggcg atctcctcga gagaaaccag
     1081 ttttcaggca ctgtccccgg tgcttccgct cgccgccgcc gcagggatcc tcggggcggt
     1141 acgtgacggt ggaaccgcag tcggagcggt cggagggggc gcggcagcag ctgcctccgg
     1201 cggggtcgtg tccggaggcg tcgccacggg actcgtcacc aagggcgtcg tggcgctggt
     1261 ggtcgccggc ggaatcgggg tgggcacggc cgaggttgct cgccaccatc gggatgcttc
     1321 gaccggacag gcagtcaccc gggaatcaag ccagacgttg ggcctcggga catcggcggg
     1381 cttcgggccg aagagtccgg aagcgaccac cggcgaccgt gcattcgggg agcacggccg
     1441 agaaggcggg aagcgagaag gccacggtga cgaaggccgg tcaggttcag gccgggagcg
     1501 gggcggctcg gaaaagaacg gccaggaatc gtccgggcat gaggccggaa atgactcttc
     1561 gggtgagcgg gtgactcccg ggcagggaca gggcaacgga cctgcggaat tgccagaagc
     1621 ttcgtcaaag ggtcaggcga gggccgacga agcctcaagc gcggtccccg gaaagacggg
     1681 gagtccgccc ccgtcgcaga agccggcgac tccgcccggg acgtcatccg gcggaagttc
     1741 cggcaactcc cagggcaatt ccggatccgc ggcaactgag agttcaggcg gcaattccga
     1801 gtccgcacca gctgtcggga agccggaatc accgggcaag tccggaaact agttccttac
     1861 tttctgccca ccggatcgtt ttctgattga acgggccgca accgcggtct tctgaaagga
     1921 gaatcccaag tgaagaagtt cgcactgaca atcgtcgcca ctgccgccct gctggctccg
     1981 gtggcctccg caaccgcagg tacccacccc gagcatcccg acaaggccaa gggtccgaag
     2041 gccggcaagg tgagcaaggc caaggcctac gggaagtact gcaagggtca gagcaagaag
     2101 cacgtcaagg gccagaaggg cactgacttc tcccgctgcg tcaacgcgat ggccaaggcc
     2161 aaccgggaag attcgatgtc tgcccgcaag gcatgcaagg cgctgagcaa gaagcacgtg
     2221 aagggtgaga agggaacccc gttcagccgc tgcgtcgtcg gcgtcgcgca gatgcgcaag
     2281 gacgcctgac ggtccacaag aacgcttgat gttccagcgc aacccccgcc caggcggggg
     2341 ttgcgtttag cgcgcccggc aggattcgaa cctgcgacct catgctccgg aggcatgcgc
     2401 tctatccagc tgagctacgg gcgctcgttc gggggagaag agcaaagagg gcgcgtgacg
     2461 aaagggcaag caccggcccg agaaaagggt tgcgggacgg gcgagcaatc ggcggatagg
     2521 ctccccgtat ggttgtcccc acgacggaga tcacggtttc ctgcgctcgt gcctcgcggc
     2581 atctgctcga ggtcgaagtg cgaacgccag gtctgctcgc cggcgcgcac gatttcgc
//
LOCUS       DAOTIP010000260         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46364_length_2629_cov_3.625097, whole genome shotgun sequence.
ACCESSION   DAOTIP010000260 DAOTIP010000000
VERSION     DAOTIP010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46364_length_2629_cov_3.625097"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..607
                     /locus_tag="P5138_05775"
     CDS             203..607
                     /locus_tag="P5138_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRV60118.1"
                     /translation="MAIGADITRPARRFRRLSRQTVHGFEVLVAGDPVSRLLGLALLD
                     RHRAPEGLLIPRCRSVHTYGMRFPLDLLFLGPDFQPLLAVREVGPGHRVRVPEAEAVL
                     ELPSDWDVEKWESATGAERSRSGPCGSGEEGS"
     gene            607..1317
                     /locus_tag="P5138_05780"
     CDS             607..1317
                     /locus_tag="P5138_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV60119.1"
                     /translation="MLPTREETARIVICEDDQPTLELLSDHLSADRFGVLAAPTAADA
                     LRLCHYDDPDLLLLDLGLPDAAGMDLLREIRNPDRSANPADPGIGVIIVSGRGTEQDR
                     VRGLNEGADDYIQKPFSYGELLARINSVLRRRRRSHPLLRVAEIEINDVEHTVKVGGK
                     HIRLSMKEYALLKILASEPTRVYSKEELLQEVWGYRSTGHTRTLEAHCSRLRRKLDPK
                     RGRYVVNCWGVGYRLVGP"
     gene            1314..2072
                     /locus_tag="P5138_05785"
     CDS             1314..2072
                     /locus_tag="P5138_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV60120.1"
                     /translation="MRGLAGVGCELNSVLPLLVAVLVIAFREVWLAHRRSLVNRALHE
                     ARRPLQVIALSLPVGSGRMIPALPVWQAIRALGEVDRQINGGAPDRIRPEPVACRLMT
                     EACARRWQSRARMVGGGIEVRWTGPEALVTGDPAMLSAAIENLILNAIEHGGPRIVVR
                     ALVVGRRLRVEVTDNGRSHRAEGRSGSPAEVVARLRGRERHGHGLAVVRDVARRHDGR
                     FDLQVDERGSSATLTLPCESRPAAPATGLVGTEG"
     gene            2078..>2629
                     /locus_tag="P5138_05790"
     CDS             2078..>2629
                     /locus_tag="P5138_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60121.1"
                     /translation="MGRRRRAIILLLAAGGAGILAVTLIGGYSSSVAESYGRLQPVVI
                     LTRGLAPGKAISPRVAAASFEIRKVPERFVPAAALSDPAPAVGMEVIGVLPAGSYLTG
                     PVLRVPESDRPEAPRLGQGRHAVEISVSGAGALAGSGKVDVLVTTDDDRGGGRTLVAA
                     RRVPLISVGQDGRSEVGPGLTEVT"
BASE COUNT          392 a          826 c          984 g          427 t
ORIGIN      
        1 ctgcgggtct ttttactttc cacggttgaa gccttgagcg ccgttggcat ggtccgcggg
       61 cgcccgggca tcatgccttg agggtgcctt tagggacttc gggacagacc acctgaacgg
      121 gcggttgcac cccgaaagac tcgttgcaga gccgatcggc ttcagcgggg gagagattcc
      181 tgtgcccgtt gcttaggagg gcatggcgat cggcgctgac atcactcgtc ccgcgaggcg
      241 gttccggcga ctctcccggc agaccgttca cggattcgaa gtccttgtcg ccggcgatcc
      301 ggtctcgcgg ctcctggggc tggctctgct ggaccggcac cgggcgccgg aaggtctgct
      361 gatcccccgt tgccgctcgg ttcacaccta cggcatgcgg ttcccgctgg atttgctgtt
      421 cctcggtccg gacttccagc cgctgctcgc ggtccgggaa gtcggaccgg gccatcgggt
      481 gcgggtcccg gaagcggagg cggtgctcga gctgccttcg gactgggacg tcgagaagtg
      541 ggagagcgcg acgggagcgg agaggtcgcg aagcggccct tgcggatccg gggaggaagg
      601 ttcctgatgt tgccgaccag ggaagagacg gccaggatcg tgatctgcga agacgaccag
      661 cccacactgg agctgctctc cgaccacctg agcgccgacc ggttcggcgt cctcgcggcg
      721 ccgaccgcgg ccgatgcgct gagactctgc cactacgacg acccggatct gctgctgctt
      781 gacctcgggc ttccggatgc ggcgggaatg gatctgcttc gggagatccg caacccggac
      841 cggtccgcca acccggccga tcccggaatc ggcgtgatca tcgtcagcgg ccgcggcacc
      901 gagcaggacc gggtccgggg gctgaacgag ggcgccgacg actacataca aaaacccttc
      961 agctacggcg aattgctggc ccggatcaat tcggtcctga ggaggaggcg gcggtctcat
     1021 cccctgcttc gggtcgcgga aatcgaaatc aatgacgtcg agcacacggt caaggtcggc
     1081 gggaagcaca tccggctctc aatgaaggag tacgcgctgc tgaagatcct cgcgagcgag
     1141 ccgacccggg tctattccaa ggaggagctg ctgcaggagg tctgggggta ccggtccacc
     1201 ggccacacca ggacccttga agcccactgc agccggctgc gccgcaaact cgatcccaag
     1261 cggggcaggt acgtggtcaa ctgctggggt gtcggctacc ggctggtcgg accgtgaggg
     1321 ggctcgccgg tgtgggttgt gagctgaaca gcgtcctgcc gctgctggtc gcggtgctgg
     1381 tgatcgcctt ccgggaggtc tggctggccc atcgtcgcag cctggtgaac cgggccctcc
     1441 acgaagccag gcgccccctg caggtgatcg cgctttcgct gccggtcggg tcgggacgga
     1501 tgatcccggc gctgccggtc tggcaggcaa tccgggcgct gggtgaggtc gaccgtcaga
     1561 tcaacggagg ggccccggac cggatcaggc cggaaccggt cgcctgccgc ctgatgaccg
     1621 aggcctgcgc gcggcgctgg caatcccggg ctcgaatggt cggcggggga atcgaggtcc
     1681 gctggaccgg gcccgaagcc ctggtcaccg gtgacccggc catgctttcc gcggcgatcg
     1741 agaacctgat cctcaacgcg atcgagcatg ggggtcccag gatcgtggtc agggcactgg
     1801 tggtcggtcg aaggttgaga gtcgaggtga cggacaacgg caggagccat cgggccgagg
     1861 gacggtcggg cagtccggct gaggttgtgg cccggctgcg cgggagggag cgccacgggc
     1921 acggtcttgc ggttgtcagg gacgtggccc gcagacacga cggtcgcttc gatctgcagg
     1981 tggacgaacg cggatcctcg gccacgctga cgcttccctg cgaatcccgg ccggccgcgc
     2041 cggctaccgg actggtcggg accgagggct agaggggatg ggccggcgaa ggcgggcaat
     2101 catcctgctg ctcgcggccg gcggggccgg gatcctcgcg gtcaccctga tcggcggcta
     2161 ttcctcgtcg gtagccgaga gctacggcag gctccagccg gtcgtgatcc tgacccgtgg
     2221 cctcgccccc ggcaaggcga tctcaccccg ggtggctgcg gccagcttcg agatccggaa
     2281 ggtgccggaa aggttcgttc cggcggctgc cctcagcgat cctgcgccgg cggtggggat
     2341 ggaagtgatc ggggtgctgc cggccggcag ctatctgacc ggtccggtac tccgggtgcc
     2401 cgaaagtgat cggccggagg ctccccggct ggggcagggt cggcatgcgg tcgagatctc
     2461 ggtctccggg gccggggcgc tggccggttc gggcaaggtc gacgtgctgg tgaccaccga
     2521 cgatgaccgg ggcggtgggc ggaccctggt cgccgcccgg cgggtcccgc tgatctcggt
     2581 cgggcaggac gggcgaagcg aggtcgggcc gggcctgacc gaggtgacc
//
LOCUS       DAOTIP010000261         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46368_length_2629_cov_3.350427, whole genome shotgun sequence.
ACCESSION   DAOTIP010000261 DAOTIP010000000
VERSION     DAOTIP010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46368_length_2629_cov_3.350427"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..510)
                     /locus_tag="P5138_05795"
     CDS             complement(145..510)
                     /locus_tag="P5138_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60122.1"
                     /translation="MPGSLKKAPLLLSGIALFLVGLTGFAAGFWVVGSEIINPDPEAD
                     GCGGLMVAVLLYLLTGCAFAGLGAVLMRSSGAGFMTRIPLIGRASPTGLGITAGVAGF
                     TLLAIALFLDYIVSAGSGS"
     gene            complement(522..902)
                     /locus_tag="P5138_05800"
     CDS             complement(522..902)
                     /locus_tag="P5138_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60123.1"
                     /translation="MSDNAFAKGVVHPALFVGGCIFSGLFLLVVAFLALSLVGTLFGQ
                     STESDLFGDPAANLAAYTLCLFSTGGLGLAMLRGSGVKWVSQVPVLRDVPTWALVAVG
                     VAASLPILLFVALIISIIAGGGLD"
     gene            complement(899..1405)
                     /locus_tag="P5138_05805"
     CDS             complement(899..1405)
                     /locus_tag="P5138_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60124.1"
                     /translation="MRDIQVATEDSPGKRRAAWLAIGAVTMSIGLGMVAFPLAAVESL
                     DGLAASMILVVPGLLWLATGIKVILRVTGRKADVLIGSTGVVAFTISAMVGVLGTFVM
                     LAFWALSHSVWVNEGGTPETAPNDGLIYAGACFLYATICLLLAFASSRIEDPPVSGLR
                     REQGSRGA"
     gene            complement(1402..1794)
                     /locus_tag="P5138_05810"
     CDS             complement(1402..1794)
                     /locus_tag="P5138_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60125.1"
                     /translation="MTERLQSQGIPCRGSRILAGTGLGILLIGLLIAVTGLANVILAL
                     TDGGPGVKGAVWGVANGVFIIALGGGFALLGLSLFRRNAPRTRGRIPGTARSNARSGF
                     LLALTALLGTGLMIVLLIWLANGIAQTT"
     gene            complement(2020..2463)
                     /locus_tag="P5138_05815"
     CDS             complement(2020..2463)
                     /locus_tag="P5138_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60126.1"
                     /translation="MIPFRDNMDLRGPVWGTLAFLLIYFVLALVGDIPHMNAWQVLVG
                     LFGLWLFAPYVERRTGTPLFVVGFLFVAGVTGFLVGAVDEATGPYAISFFLPVLATAG
                     VHIALAPRSKILCLIPVPFAMTFVEVPTIAMTIIWLALEMLLTAA"
BASE COUNT          546 a          825 c          864 g          394 t
ORIGIN      
        1 tgatcgcctg gttcctccgg tacctgacca gccattcgat gtcgctcttc gtctggtacc
       61 gcctgatcgt cggcgccgga atcctcaccc tcaccgccgc cggcctgatc tcggtctgaa
      121 ccccggtcgg cctgcccgga ccttctagga cccggagccg gcggagacta tgtagtcgag
      181 aaagagagct atcgcgagga gcgtgaatcc cgcgacgccc gccgttatcc ccaacccggt
      241 tggggaggcc cttcctatga ggggaatcct ggtcatgaaa cccgctccgc tggaacgcat
      301 gagaactgct ccgagtccag cgaaagcaca gccggtcaac aggtacaaga gcacggcgac
      361 catgagcccg ccgcacccgt cggcttcggg atccgggttg atgatctcgg acccgacgac
      421 ccagaaaccg gcggcgaagc cggtcagccc cacgaggaag agagcaattc ccgacaggag
      481 gagtggcgct ttcttcaggc tgccgggcat ttcatccggg gctagtcgag accgccaccg
      541 gcaatgatcg aaatgatcag ggcgacgaac aggaggatcg gcaggctcgc ggccacgccg
      601 acggctacaa gagcccaagt gggcacatcc cgcagcacgg gaacttgact tacccacttg
      661 acaccgctgc cccggagcat tgccaggccg agaccgcctg ttgaaaacag acagagcgtg
      721 taagcagcaa ggttggctgc cggatcaccg aacaggtcgg actcggtgct ctggccaaac
      781 agcgtgccaa ccagggacag ggcgaggaag gcaacgacaa ggaggaacag gccagagaaa
      841 atgcagcctc caacgaacaa tgcagggtgg accaccccct tggcgaaggc gttgtcgctc
      901 atgcgccccg actcccttgc tcccgcctca acccggaaac gggagggtct tcgatccggc
      961 tggatgcgaa agcgaggagc agacagatgg ttgcgtacag gaagcaggcg cccgcgtaga
     1021 tcagaccgtc gttcggggcg gtctccgggg tgcccccttc attcacccag accgaatgcg
     1081 aaagggccca gaaagcaagc atcacgaagg tgcccagcac accgaccatg gcgctgatcg
     1141 tgaaagcgac gaccccggtg ctgccaatca gcacatccgc ttttctcccg gtaacgcgca
     1201 ggatcacttt gatgccggtg gcgagccaaa gaaggccggg gaccacgagg atcatcgaag
     1261 ccgccaaccc gtccaggctt tccaccgcgg cgagggggaa agcgaccatc cccagaccga
     1321 tcgacatcgt gaccgcgccg atggccagcc acgcggcccg gcgcttcccg ggggaatcct
     1381 ctgtcgcgac ctggatgtcc ctcatgtcgt ttgagctatc ccgttcgcga gccagatcag
     1441 aagaacgatc atcaggccgg tcccgaggag cgctgtcagg gcgagcagga atccgcttcg
     1501 tgcgttcgac cgggcggttc cgggaatccg gcccctcgtc cggggagcgt tccgccgaaa
     1561 caggctcagg ccgagcagcg cgaaaccacc gcccagggcg atgatgaata cgccgttggc
     1621 cacaccccag accgcgcctt tcacgcctgg gccgccgtcg gtcagcgcca agatgacgtt
     1681 ggcgaggccg gtgactgcga tcagcaggcc gatcaggagt atccccagac cggtcccggc
     1741 gaggatcctg ctgccacgac aaggaattcc ctggctctgg agtcgttccg tcacggtcct
     1801 gagtaccccg gatgtttcga gcggttaccg aagctcggtt caattcaatg gggctatatc
     1861 cgagcagatt gaacccaagc tggttctggt cccggtcggc cacccgatgc cagccactcg
     1921 gttcgaatta cccccgatat ggagcggtga tccacaccga gccggctctc gttcccagac
     1981 tagggcggtg aagcgatccc gggccaggtc aggtgggggt caggcggcgg tgaggagcat
     2041 ctcgagggcc agccagatga tcgtcatggc gatcgtcggt acctcgacga aggtcatcgc
     2101 gaaaggcacc gggatcaggc agaggatctt cgagcgggga gcaagggcga tgtggacgcc
     2161 ggccgtggcg agcacgggga ggaagaagct gatcgcgtat gggccggtcg cttcgtcaac
     2221 cgcgccgacc aggaagccgg tgacgccggc cacaaagagg aaaccgacca cgaacagcgg
     2281 ggtgccggtc ctgcgttcca catagggggc gaacagccag agtccgaaca gtccaaccag
     2341 cacctgccag gcgttcatgt gcgggatgtc gccgaccagg gccaggacga aatagatcag
     2401 aaggaaggcg agcgtgcccc agacgggacc gcgcaggtcc atgttgtcgc gaaacgggat
     2461 catcggatag ggctttcgtt gggcaagatc aacgggccgc gagggccagg gtgagcctgc
     2521 gagggtatcg ctgaaaggga ccgggccgct ttgcgggatc ggcggggtgg gcagccccgg
     2581 gatcaggcag cttcggcggc cttgcgggcg ctttcgtccg cgtggtagg
//
LOCUS       DAOTIP010000262         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46593_length_2620_cov_3.164522, whole genome shotgun sequence.
ACCESSION   DAOTIP010000262 DAOTIP010000000
VERSION     DAOTIP010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46593_length_2620_cov_3.164522"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="P5138_05820"
     CDS             complement(<1..1737)
                     /locus_tag="P5138_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRV60127.1"
                     /translation="MSNRLADETSPYLLQHQDNPVDWQPWGPEALSEARERQVPILLS
                     VGYAACHWCHVMERESFEDEATAAYMNEHFVPVKVDREERPDIDAIYMEAVQSITGQG
                     GWPLTAFLDPAGVPFHGGTYFPPDDSRGMPSFMMVMEAVIKAWDERRDEILAQSGDVR
                     TRLGAIALIGAPEGDLDPELAARRAGNLAEQADMDLGGFGPAPKFPPASTLDFLLGQG
                     ETAAVEVTLDRMAAGGIFDQLAGGFARYSVDDRWLVPHFEKMLYDNALLARTYLRAWQ
                     VTGHDRYRWVAQKTLDWAMSEMLDASGGFYASLDADSEGVEGLFYTWTPAEIGEALGG
                     RAPAVIEAFGVTEEGHLDGRSVLHLPAGVPPDEIEPGFEELSDELLAARTKRVRPALD
                     DKVISSWNSLMVSALAEAGAVLERPDYLEAAHRTADFIWNGMRGTQGNLLRTWRHGEA
                     RLNAYLEDHAFLLEALLDLYQADFNPAVFDRARAVAETMIDRFADPARGGFFTTSHDH
                     EELIARRKDVGDHPIPSGSSSAALGLLKLSALTGEATYRRKAEEVLKLLAPAIARQPS
                     AFGHLLQALALYG"
     gene            1793..2596
                     /locus_tag="P5138_05825"
     CDS             1793..2596
                     /locus_tag="P5138_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60128.1"
                     /translation="MAIEATIFGDFWRNQIESNHQEAIFLVLIGFLLSFGFIRMSTRL
                     MRSPKVPWWPGSIVSDGGLHVHHHVFGIVMMMISGSVAFALDGNSLGFNICALAFGIG
                     IGLTIDEFALWLHLEDVYWSEEGRRSIDATVLTALVLAMIMLGARPFEIGSDSVGILI
                     ASLITVLFIFIVVGICFLKERVMHAFIGFFIWPVALYGACRLGKPTSPWAKRRYGEKN
                     PSKQKRAEARFRPGRRTDRFKERFRDLVGGGLIHSADDEKETPPSSDVS"
BASE COUNT          435 a          838 c          871 g          476 t
ORIGIN      
        1 gccgtagagg gcgagagcct ggagcaggtg gccgaaggcg gaaggctggc gcgcaatcgc
       61 cggcgccaac agcttgagca cttcctctgc cttgcggcgg taggtcgctt cgccagtcag
      121 ggccgagagc ttcagaagtc ccagagcggc cgacgaggaa ccagagggga tggggtggtc
      181 accgacatcc ttgcgccggg cgatcagttc ctcatggtcg tgcgaggtgg tgaagaagcc
      241 gccgcgggcg gggtcggcga agcggtcgat catcgtctcg gccacggccc tggcgcggtc
      301 aaacacggcc gggttgaaat ccgcctggta caggtcgagc agcgcctcga gcagaaaggc
      361 gtggtcctcc agatacgcgt tgagtcgggc ttcgccatgt ctccaggtgc gcagcaggtt
      421 gccctgggtg ccgcgcatcc cgttccaaat gaagtcggcc gtgcgatggg ccgcctcgag
      481 gtagtccggc cgctcgagca ccgcgcccgc ctcggcgagg gccgagacca tcagcgagtt
      541 ccatgagctg atcaccttgt cgtcgagtgc cggccgtacc cgtttcgtcc tcgccgccag
      601 aagttcgtcg gaaagctcct cgaaccccgg ttcgatttcg tccggtggaa cgccagccgg
      661 aaggtggagc acgctgcgtc cgtccaggtg gccttcctcc gtcacgccga aggcctcgat
      721 cacggccgga gcccggcctc cgagcgcctc cccgatctcg gccggggtcc aggtgtagaa
      781 gaggccttcg acaccctccg agtccgcgtc cagcgaagcg tagaaacctc ccgaggcgtc
      841 gagcatctcc gacatagccc agtccagcgt tttctgggcc acccagcggt accggtcgtg
      901 gccggtcacc tgccacgccc gcaggtaggt gcgggccagc agggcattgt catagagcat
      961 cttttcgaag tgggggacca gccacctgtc gtcgaccgag tagcgggcga agccgccggc
     1021 cagctggtcg aaaattccgc cggccgccat ccggtcaagg gtcacttcga cggcggccgt
     1081 ctcgccctgg ccgagcagga agtcgagggt cgaagccgga gggaacttcg gggcggggcc
     1141 aaaaccgccc aggtccatgt ccgcctgctc tgcgaggttg cctgcccgtc gcgccgccag
     1201 ttccgggtcg aggtctcctt cgggcgcacc gatcagggcg atcgcgccaa gcctggtccg
     1261 cacgtcaccg gactgggcga gaatctcgtc gcgccgctcg tcccatgcct tgatcaccgc
     1321 ttccatcacc atcatgaagc tcggcatgcc gcgggaatcg tcgggcggaa agtaggtgcc
     1381 gccgtggaaa ggaaccccgg ccgggtcgag gaaagcggtc agcggccagc ctccctggcc
     1441 ggtgatcgac tggaccgcct ccatgtagat cgcatcgatg tcgggcctct cctcgcggtc
     1501 caccttgacc gggacgaagt gttcgttcat gtaggcggcc gttgcctcgt cctcgaagga
     1561 ttcccgctcc atcacatggc accagtggca ggccgcgtag ccgacagaga gcaggattgg
     1621 cacctgcctt tcccgggcct cggacagggc ttcgggcccc cagggctgcc agtcgacggg
     1681 gttgtcctga tgctggagga ggtaggggct ggtctcgtct gccagtcggt tgctcatggg
     1741 cacatcatcg aatatcgggc attccggcgt ttcgcatggc aggattcagg tgatggcaat
     1801 cgaggcaacg atcttcggcg acttctggcg caaccagatt gagagcaacc accaggaggc
     1861 gatcttcctg gtcctgatcg ggttcctgct ctcgttcggc ttcatccgga tgagcacccg
     1921 gctgatgcgg agcccgaaag tcccgtggtg gccgggcagc atcgtcagcg acggtggcct
     1981 ccacgtccat caccacgtgt tcgggatcgt gatgatgatg atctccggtt ccgtggcctt
     2041 cgcccttgac ggcaacagcc tcgggttcaa catctgcgcg cttgccttcg gtatcgggat
     2101 cggtctgacc atcgacgagt tcgcgctgtg gctccacctc gaggacgtct actggtcgga
     2161 ggaaggcagg cgttcgatcg atgcgacagt gctcacagca ctggttctcg caatgatcat
     2221 gctcggggcg aggcccttcg agatcgggtc ggactcggtc gggatcctga tcgccagcct
     2281 gatcactgtc ctcttcatct tcatcgtggt cgggatctgc ttcctcaagg aacgggtcat
     2341 gcatgccttc atcggcttct tcatctggcc ggtcgcgctc tacggggcct gccgtctggg
     2401 caagccgacg tcaccctggg ccaagcggcg ctacggggaa aagaatccgt ccaagcagaa
     2461 gcgggccgag gcgagattcc gacccggacg caggacggat cgtttcaagg agaggttccg
     2521 ggacctcgtc ggcggtggat tgatccactc cgccgacgat gagaaggaga cgccgccctc
     2581 gagcgacgtc tcctgaatca tcaggcggtt acgtcctggg
//
LOCUS       DAOTIP010000263         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46711_length_2615_cov_3.507031, whole genome shotgun sequence.
ACCESSION   DAOTIP010000263 DAOTIP010000000
VERSION     DAOTIP010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46711_length_2615_cov_3.507031"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5138_05830"
     CDS             <1..518
                     /locus_tag="P5138_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60129.1"
                     /translation="AGLLENGFHGILNLIWSDNMDQGWGATVKVAPDGSFLTAGDGIN
                     RIGHVGPDDPGLVTTCRRKPGTFKLLGVKFGPKRTWARLRVSVPEAGRLSVKGRKVIA
                     RTKSGKAAGTYRIAVLAKPRYLKPSRRPRIVKVPVRISFSGASTEVSKRITIRLKSRK
                     RQATRSSREGT"
     gene            563..>2615
                     /locus_tag="P5138_05835"
     CDS             563..>2615
                     /locus_tag="P5138_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60130.1"
                     /translation="MAVLFACLTIQAQPANAGLEKTAEWGGSSFAAFLSLQSVALDSN
                     GNAYVSEYGQNRIIRVGIEDVADLNWQVSSMPDPARLFDIRIDLAIDDSDHIYATDNL
                     TGTIRKFSTNGDLLASWTPAVPDGTVLQSIAWESGSVWVLTNTEVLRFAPDGTPQGSW
                     GGFESSCCAANPIIRGDGNGHLVIAALKVEPGVTSATKSVYKFDPDGTLISKWSLPAV
                     IIQQWPGSYPIGYSGLAVDGSGDIWVSSQGMDPAIQKIGPGGLEGPGVDTPYSASPNA
                     MAIDTGGNFWTVLLGGLKRIDASGDVTGTWGKKDFPRYAFDTTGQVGSVAALAAGSDG
                     TVGVFDYELERVHTFTAAGDPLTRVEDIIGSPPAKEAVNLYLADDGTIEIFNRVTEKL
                     TRFDSDGNLLGETDFPLEGAIRDFNRDPSGGYVAVGGNPTRIVRFDSAGTETASWPSV
                     SSFYDHGGLPWYGGKVVADSSGRIYATDGSRIDAYSSTGDFRESLKILEDECVGYGAA
                     IMDLAVDSEDNLYALVQPQDDDGQVAVFKFNPDLQEAWSEETPGYQTHLAVGPDGSIY
                     LGGRGKVSRYTASEPLGSLEPPVCEIEPVPPVGDFKVRKVVFGPGRHWAKVRVYAPEK
                     GRITLSGRKAITRRVTAVRSGVYPVSITVRPRYLMTARKVRRFKVSVRIASTRRSFRR
                     DP"
BASE COUNT          500 a          801 c          867 g          447 t
ORIGIN      
        1 tcgcgggcct tcttgagaac gggttccatg gcatactgaa cctgatctgg tccgacaaca
       61 tggaccaggg atggggcgcc accgtgaagg tggccccgga cggctcgttc ctgaccgccg
      121 gcgacgggat caaccgcatc gggcacgtcg gaccggatga tcccgggctc gtcacgacct
      181 gccgcaggaa gccaggcact ttcaaactgc tcggagtgaa gtttggcccg aagcggacct
      241 gggcccgact gcgcgtctcg gtgcccgagg caggcagact cagcgtgaag ggtcgcaagg
      301 tcattgcccg cacgaagtcg ggcaaggccg caggcaccta ccggatcgcg gttctcgcga
      361 agccccggta cctgaagccg tccaggcgcc cgcgaatcgt gaaggtgccc gtgagaatca
      421 gcttctccgg agcatcaacc gaagtctcga agcggatcac gatcaggctg aaaagccgga
      481 aacgccaggc gacccggtct tcccgggaag ggacatgact ccagtccgcg gtcaagagtt
      541 tgcgagagga ttcctcggcc tcgtggcggt cctcttcgcc tgcctgacga tccaggccca
      601 gccggcgaac gccggcctcg aaaagacagc cgagtggggt gggagcagct ttgcggcgtt
      661 cctgtcgctg cagtcggtcg cgcttgacag caacggcaat gcgtacgttt cggagtacgg
      721 acagaaccgg atcatccggg tcggaatcga agatgtcgcc gacctgaact ggcaggtgag
      781 ttcgatgccg gatccagcca ggctgttcga tatccggata gatctcgcaa tcgatgacag
      841 cgaccacatt tacgcgaccg acaatctgac cggaacgatc aggaagttct cgaccaatgg
      901 agacctgctg gccagctgga cgcccgcggt cccggacgga acggtcctac agtcgatcgc
      961 ctgggaatcc ggatcggtct gggtgttgac caacacggaa gtcctgaggt tcgcccccga
     1021 cggaacaccc cagggaagct ggggtgggtt cgaatcgagc tgctgcgcgg cgaatccgat
     1081 catccggggt gacgggaacg gtcacctggt catcgctgcc ctgaaggtcg agcccggcgt
     1141 cacttcggcc accaagtctg tctacaagtt cgaccccgac gggaccctga tctcgaagtg
     1201 gtcgctgccc gccgtgatca tccagcaatg gccggggtcc taccccatcg gctactcggg
     1261 cctcgcggtc gatgggtcgg gcgatatctg ggtgtcgtcg caggggatgg acccggctat
     1321 ccagaagatc ggccccggcg gactcgaagg gcctggagtc gacactccgt acagcgcctc
     1381 gccgaatgcc atggcgattg acaccggggg gaatttctgg acggtgctcc tcgggggcct
     1441 gaaacggatc gatgcctccg gggatgtcac gggaacctgg gggaagaagg acttcccgag
     1501 gtacgccttc gacacgaccg gtcaggtcgg ttcggttgcg gccctcgcgg cggggtccga
     1561 cggaaccgtc ggggtcttcg actatgaact ggaaagggtc cacaccttca ctgcagccgg
     1621 cgatcccctc accagggttg aagacatcat cggttccccg cccgcgaagg aagcagtgaa
     1681 cctctacctg gccgatgacg gcacgatcga gattttcaat cgagtcacgg agaagctcac
     1741 ccgcttcgac agtgacggaa accttctggg agagaccgac ttcccgctcg agggagcgat
     1801 ccgggatttc aaccgcgacc cgtccggcgg atacgtcgcg gtggggggga acccgacccg
     1861 gatcgtccgc ttcgactccg cgggaactga aacggccagt tggccttcgg tctcgagttt
     1921 ctacgaccat gggggtctgc cctggtacgg ggggaaggtc gtggccgatt cgagtggccg
     1981 catctatgcg acggatggct cccggatcga cgcctactcg tcgacgggtg atttcaggga
     2041 aagcctgaag atcctcgaag acgaatgcgt cggttatggc gccgccatca tggacctggc
     2101 ggttgactcc gaggacaacc tctatgccct ggttcagcca caggacgatg acggccaggt
     2161 cgccgttttc aagttcaacc cggaccttca ggaagcatgg agcgaggaga ctccgggcta
     2221 ccagacccat ctcgcggtcg gacccgatgg ttccatctac ctcggcggcc gcggaaaggt
     2281 aagccggtac acggcctccg agccgctggg gtcactggaa ccaccggttt gcgagatcga
     2341 accggttccg ccggtcggcg atttcaaggt gaggaaagtc gttttcggcc ccggccggca
     2401 ctgggcgaag gtcagggtct atgcgccgga gaagggccgg atcacactca gcggcaggaa
     2461 ggcgatcacc cgccgggtga cggcggtccg gagtggcgtc tacccggtgt cgatcactgt
     2521 caggccccgt tacctgatga ccgcccggaa ggttcgacgg ttcaaggtct cggtgcgcat
     2581 cgcgagcacc aggcgatcgt tccggaggga ccccg
//
LOCUS       DAOTIP010000264         2608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46873_length_2608_cov_3.566001, whole genome shotgun sequence.
ACCESSION   DAOTIP010000264 DAOTIP010000000
VERSION     DAOTIP010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46873_length_2608_cov_3.566001"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..489
                     /locus_tag="P5138_05840"
     CDS             76..489
                     /locus_tag="P5138_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRV60131.1"
                     /translation="MHESPEIEGLEQLRDVHVAALNDGDADAWAECFAADAVQMPPNM
                     PANVGIDVIREWSRGFLGAFNAEFSLDPTEAVEAGPDWVIESGAYEIALTPNGGEGEP
                     LRDHGKYITVYQREGERWKMARDIWNSDVPLPAQT"
     gene            complement(607..1068)
                     /locus_tag="P5138_05845"
     CDS             complement(607..1068)
                     /locus_tag="P5138_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60132.1"
                     /translation="MDEEINYRSAIKDFLGRPIPPEGELRIWLDDDPVDREAPEGWIH
                     VRSVREACFALLTGRVVELSLDNDLDNPEGSETTFGTGYQVIDFLEEQEGVAGNPLWP
                     RDGIVLHTANANGRERMALSFEPLKRNPELTVREDKTPGGKPRFSVGRKTD"
     gene            complement(1061..1471)
                     /locus_tag="P5138_05850"
     CDS             complement(1061..1471)
                     /locus_tag="P5138_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60133.1"
                     /translation="MSESGGGSWKDRPIDVRAEARREVTGQRKREIVGRSVSHPPETA
                     SARQAGSRSGRFYVRDPEDYPTYPKNGRFRYSGLAKGYMYRWDLFLTGKTDEVPPTFR
                     EYSKNPGEAMKDTRRVEAAFRLGGQRSNKEQEHG"
     gene            complement(1468..1683)
                     /locus_tag="P5138_05855"
     CDS             complement(1468..1683)
                     /locus_tag="P5138_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60134.1"
                     /translation="MSASRVNCRDARSWIRDPADYPNFPLAKARRGARSNPVALAHLD
                     GWFEFLAGFRDKPPASHTTGSLEGAAR"
     regulatory      complement(1692..1782)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1887..2414
                     /locus_tag="P5138_05860"
     CDS             1887..2414
                     /locus_tag="P5138_05860"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HRV60135.1"
                     /translation="MADRAQRANVTTPAGWRPDPDDPRRLRWWDGMRWHDLTVPAPDE
                     QKVPPPPDGLIVQIKAASPSFWAALGFGVYFVVALAIADATADEAGSYGSALFVALIG
                     LALLLIFCVAPIVIIGGYIVWVIYQSVTESMPTREERRERRRADRAARRAGLPTQRQA
                     RRQRKREERLKRARR"
     gene            complement(2425..>2608)
                     /locus_tag="P5138_05865"
     CDS             complement(2425..>2608)
                     /locus_tag="P5138_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60136.1"
                     /translation="VIMTGDGSDSPHQIGGSVWKNLETAVKVSGAETRVMAVAYDNKG
                     ERLGQSALAPIGKQVR"
BASE COUNT          440 a          852 c          809 g          507 t
ORIGIN      
        1 gtcgcggccg agatcctcgg gccgcccggg atcccggact gaaattacgg acctcagata
       61 cgagaaagga ctgacatgca tgaatcaccg gaaatcgagg gactggagca gctgcgcgac
      121 gttcacgtcg cggcgctgaa cgacggggac gctgacgcct gggcggaatg ctttgccgcc
      181 gacgcggtac agatgccgcc gaacatgccg gccaacgtag ggatcgacgt tatccgcgaa
      241 tggtcgcgcg gattcctcgg cgcattcaac gccgagttct ccctggaccc gaccgaggcg
      301 gtagaggcag gaccggactg ggtgatcgaa agcggcgcct acgagatcgc gctgacgccg
      361 aacggcggcg agggggaacc gctgcgggac cacggcaagt acatcaccgt gtatcaacgc
      421 gagggggagc ggtggaagat ggcccgggat atctggaaca gcgacgtccc ccttccagcg
      481 cagacctgat cgccgggagg ccgctggccc tcggtgacgt ctcgacacag gaagccccgg
      541 aactctgact gaatcgaatg accgggatgg gcgccatcac atcggatgcc catcccggtc
      601 ttctggttaa tcggttttcc ggccgacgga gaaacggggt ttcccaccgg gtgtcttgtc
      661 ctccctgacg gtcagctcgg ggttcctctt gagtggctcg aagctcaggg ccattcgttc
      721 gcgaccgttg gcgttcgcgg tgtgaaggac gatgccgtcg cgcggccaga gtggattgcc
      781 ggcaactccc tcttgttcct cgaggaagtc gatcacctgg tagccagtgc cgaacgtggt
      841 ctcgctgcct tccggattgt ccaggtcgtt gtcaagcgag agttccacca cccttccggt
      901 caagagggca aagcaggcct cgcgaaccga gcggacgtgg atccatccct cgggggcctc
      961 acgatccacg gggtcatcgt ccagccagat tcggagctcg ccttcgggcg ggatcgggcg
     1021 accaaggaag tccttgatgg ctgacctgta attgatctct tcatccatgt tcttgttcct
     1081 tgtttgagcg ttgaccaccc aatctgaacg ccgcttcgac tcttcgtgtg tctttcatgg
     1141 cttccccggg gttcttgctg tactccctga aggttggcgg cacttcatca gtcttcccgg
     1201 tcaggaagag gtcccaccga tacatgtatc ccttcgccag gccgctgtag cgaaaccggc
     1261 cgttcttcgg gtaggtcgga taatcctccg gatcccgcac atagaacctg ccggacctgc
     1321 tgccggcctg tctggccgac gcggtctcgg ggggatggga tacggaccga ccaactattt
     1381 cccgtttccg ctggccggtc acttcccttc gggcttcagc ccgaacatcg atcggccggt
     1441 ccttccagga tccacccccg ctttcgctca tcgggccgct ccttccagag aaccggtggt
     1501 gtggctggcc gggggcttgt ccctgaaccc ggccaggaac tcgaaccatc cgtccagatg
     1561 ggcgagcgcg accggattgg accttgcgcc tcgccgcgcc ttcgccagcg gaaagttcgg
     1621 atagtcggcc ggatcgcgga tccagcttct cgcatcacgg caattcactc tgcttgcgct
     1681 cacttacttc ctttccatcg tctcgggctt tcgcaccttt cccttccggg gaggttgcgg
     1741 cggtgtcaaa gggcctcgtc cctcaaccgc tctctatgga caccgatgcc tgagtcgcac
     1801 cggaacattc aattctgaca gcgacgtttc ctagcgacaa gggcgagcgt tgtcaggtcc
     1861 atcccctggc cggcgacctc cccgtaatgg ccgatcgggc acagcgagcg aatgtgacca
     1921 ccccggcggg ctggcgaccc gacccggatg acccccggcg gctgcgctgg tgggacggaa
     1981 tgcgctggca tgacctcacg gtgcccgccc ctgacgaaca gaaggtgccg ccgcccccgg
     2041 acggcctgat tgtccagatc aaggccgcct cgccgtcgtt ctgggcggct ctgggcttcg
     2101 gggtctactt cgtggtcgcc ctggcgatcg ccgacgcgac tgccgatgaa gccgggagct
     2161 acggttcggc actttttgtc gccctgatcg gcttggccct gctgctcatc ttctgcgtgg
     2221 ccccgatcgt gattatcggc ggctacatcg tctgggtcat ctatcagtcc gtcaccgaat
     2281 cgatgccgac tcgcgaagaa cgaagggagc gccgccgtgc ggaccgggca gcgcgccggg
     2341 ccggcctgcc gacccagcgt caggcaagac gccagcgaaa gcgcgaagaa agactcaaac
     2401 gcgcccgccg gtgaacgatt ttgatcagcg aacctgcttg ccgatcggag ccagggctga
     2461 ctggcccagc ctttctccct tgttgtcgta ggccacggcc atgaccctcg tttcggcccc
     2521 gctcaccttc acggccgtct cgaggttctt ccagacggag ccgccgatct ggtggggcga
     2581 atcggacccg tcaccggtca tgatcacc
//
LOCUS       DAOTIP010000265         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46963_length_2605_cov_3.829020, whole genome shotgun sequence.
ACCESSION   DAOTIP010000265 DAOTIP010000000
VERSION     DAOTIP010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46963_length_2605_cov_3.829020"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1988)
                     /locus_tag="P5138_05870"
     CDS             complement(192..1988)
                     /locus_tag="P5138_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HRV60137.1"
                     /translation="MSDTYKPTNRSRTLADLLPNSAAVHGSDGAFLFKNDAGSWEESS
                     YDQVLADVQVLTLGLKSLGIQRGDKVAIIGNTRPEWTLFDFAAMSTGATVVPIYQSNS
                     PAECKYVLEHSEARAVVVENEEQLVKVREIKDSLPALENVVIMIGEADDAIGMDEIRK
                     MGESAAPGDFKASYEAVEPDDLCKIVYTSGTTGPPKGCMISHGNYRAMLDMADSVSII
                     EEGETSYLFLPLAHVFALLIQYGMIDIGGRIAYWERDQLKIVPNLAEVKPESFPSVPR
                     IFEKVHDTAVASAESEGGLKAAIFRWAFRVGKRYRKLEAEGKKPGFLLTKQFNFADKA
                     VFSKIRGIFGGNLRVAISGAAPIDPEILRFYQAAGVPLFEAWGMTETSTGGTTNMPGA
                     NRVGSVGRPVDGVQLKVAEDGELLLKGANVFQGYYKNPEATAETITDGWLHTGDVASI
                     DEDGYVSIIGRKKEIIITAGGKNITPVNIEQEVKRHPLVSQCVVIGDRRPYLIALITL
                     DQEALTRFAAEQGMLDEPAAMINSPLVREALEQHIEEVNKNFARVEQIKRFHILPHDL
                     SQEGGELTPTLKIKRPVIATKYEKEIDELYAS"
     gene            complement(2050..>2605)
                     /locus_tag="P5138_05875"
     CDS             complement(2050..>2605)
                     /locus_tag="P5138_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRV60138.1"
                     /translation="SRALTDAMTDRLRVAFAGNLNLAGYHLPLDAHPEIGNNALILRQ
                     LGFRPAPPGSGRIGEVRGRYIGAVGHREEPIRADEFFSLVATELDREPLVFDAGPEEI
                     STIAVVTGAAASDIHGAIALGLDAYLTGEPAEHVMADAREGGIHFIAAGHYATETCGI
                     RKLGDLVAERFGVEHEFIDVPNPI"
BASE COUNT          421 a          792 c          864 g          528 t
ORIGIN      
        1 agcgcacggt gtaacaagtt ttgtgcgttg tctgcgggaa atgtgtttcg cctccgctgg
       61 cctggtgcca ggagccacct tcccggaccc ggcccaagag ctcggcgctg caggaacgcc
      121 gggaccttac tcccggagcg ggctccaacc tgaggatgtc actcggttcc gcggcctaag
      181 ggcagcccgg gtcaggaggc gtagagctcg tctatttcct tctcgtactt ggtcgcgatg
      241 accggccgct tgatcttgag ggtcggggtg agctcgccgc cttcctggct caggtcatgc
      301 gggaggatgt ggaaacgctt gatctgctcg acccgggcga agttcttgtt gacctcttcg
      361 atgtgctgct caagcgcctc gcggaccagc gggctgttga tcatcgcggc cggttcgtcg
      421 agcatcccct gctcggctgc gaaccgggtg agggcctcct ggtcaagggt gatcagggcg
      481 atcaggtagg gccgacggtc accgatcacc acgcattggg aaaccagcgg gtgccgcttg
      541 acttcctgtt cgatgttgac cggggtgatg ttcttgccgc cggcggtgat gatgatctcc
      601 ttcttgcggc cgatgatcga gacgtatccg tcttcgtcga tcgaggcaac gtcaccggtg
      661 tgaagccagc cgtcggtgat cgtttcggcg gttgcttcgg ggttcttgta gtagccctgg
      721 aagacgttgg cgcccttcag cagcagttcg ccgtcctcgg cgaccttcag ctggaccccg
      781 tcgacgggcc ggccgaccga gccgacccgg ttggcgcccg gcatgttggt ggtgccaccg
      841 gtcgaggtct cggtcatgcc ccaggcctcg aaaaggggca cgccggccgc ctggtagaag
      901 cggaggatct ccgggtcgat cggggctgcc ccgctgatcg caacccgcag gttgccgccg
      961 aagatgccac ggatcttcga aaagacggcc ttgtcggcga agttgaactg cttggtcagg
     1021 aggaagccgg gctttttccc ttccgcttcg agcttgcggt aacgcttgcc gacgcggaag
     1081 gcccagcgga agattgccgc cttcaggccg ccctccgatt cagctgaggc aaccgcggtg
     1141 tcgtggacct tttcgaagat ccgcggaaca gaggggaaag actccggctt gacttcggcg
     1201 agattcggga cgatcttcag ctggtcgcgt tcccagtagg cgatcctgcc gccgatgtcg
     1261 atcatcccgt actggatcag cagggcgaaa acgtgggcga ggggcaggaa gaggtaggag
     1321 gtctcgcctt cttcgatgat gctcaccgag tcggccatgt cgagcatcgc ccggtagttg
     1381 ccgtgggaga tcatgcagcc cttcgggggg ccggtggtgc ccgaggtgta gacgatcttg
     1441 cataggtcat ccggctcgac cgcttcgtaa ctcgccttga agtcgccggg ggctgcggac
     1501 tcgcccatct tgcgaatctc gtccatgcca atcgcatcat cggcttcgcc gatcatgatc
     1561 acgacgttct cgagggccgg cagggaatcc ttgatctcgc ggaccttgac gagctgctcc
     1621 tcgttttcaa ccacgaccgc gcgcgcttcg gagtgctcga gcacgtactt gcattcggcc
     1681 ggcgagttgc tctggtagat cggcacgacg gttgcgccgg tgctcatcgc cgcgaagtcg
     1741 aacagcgtcc actcgggccg ggtgttgccg atgatcgcga ccttgtcgcc acgctggatg
     1801 ccgagcgact tgagaccgag ggtcagtacc tgtacgtcag ccagcacctg gtcgtaggag
     1861 gactcttccc agctgccggc gtcgttcttg aagagaaagg caccgtccga gccgtggacc
     1921 gctgccgaat tcggcagcag atcggcaagg gtccgggaac ggttcgtcgg cttgtaggtg
     1981 tcggacaact ctctctcctg gttggctatg tggcgtcgct aggcaattcg atcgattgat
     2041 ccttccacat cagatcggat tgggtacatc aatgaattca tgttcgaccc cgaatcgctc
     2101 cgcgaccagg tcgccgagct ttcggatccc gcaggtctct gtcgcgtagt ggcccgccgc
     2161 gatgaagtga attccgcctt cgcgggcgtc cgccatcaca tgttcggccg gctcgccggt
     2221 caggtaggcg tccagcccta acgcaatcgc cccgtggatg tcggaggcgg cggccccggt
     2281 cacgactgcg atcgtcgaga tttcctcggg gccggcatcg aacaccagcg gctcacggtc
     2341 gagttcagtg gcgaccagtg aaaagaactc atcggcccgg atcggctctt cccggtggcc
     2401 gacagcgccg atgtagcggc ccctgacctc accgatccgg ccggatccgg gcggggccgg
     2461 acggaatccg agctggcgca ggatcagtgc gttgttgccg atttcgggat gggcgtcgag
     2521 cggcaggtgg tacccggcca gattgaggtt cccggcgaag gcaacccgaa gccggtctgt
     2581 catggcgtcg gtcagggccc gcgaa
//
LOCUS       DAOTIP010000266         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46972_length_2605_cov_2.907059, whole genome shotgun sequence.
ACCESSION   DAOTIP010000266 DAOTIP010000000
VERSION     DAOTIP010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46972_length_2605_cov_2.907059"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="P5138_05880"
     CDS             complement(<1..279)
                     /locus_tag="P5138_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV60139.1"
                     /translation="MSLFNRIVVGTDGSDSAREAIRQAAALAQMSGASLDVVSAYEPV
                     PDRRSKEETSGAPSDVSHEFGPREDVNFALDGAASIAADFDVEIGRHPM"
     gene            complement(332..481)
                     /locus_tag="P5138_05885"
     CDS             complement(332..481)
                     /locus_tag="P5138_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60140.1"
                     /translation="MPTNWRILMIVICVFLVASVTIGTIQLLSTPTEIFGPDFQGLPK
                     QEFPR"
     gene            532..1275
                     /locus_tag="P5138_05890"
     CDS             532..1275
                     /locus_tag="P5138_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV60141.1"
                     /translation="MNAKRAVPKGLEEVADGVWLLTGDIKKAMNIYFIEGPDGEIVQF
                     DAGTRPMTKRNRRAIEEFGGIDRVVLGHAHADHRGTAPGLGVPVWCHPDEVADATSSA
                     SIAPYMNLRELEFAPVRWIYPFLLRRWDGGPVEIAGTVEEGDEVAGFKVIHFPGHAPG
                     LIGLWRESDRVAVVSDVVYFVDSARLKPLPEGEASVPHPAWAWDHQKAKESVRKLAAL
                     NPRVVAAGHEHPLRGDDLSDVLLRAADKY"
     gene            1330..>2605
                     /locus_tag="P5138_05895"
     CDS             1330..>2605
                     /locus_tag="P5138_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60142.1"
                     /translation="MAGSLVIGRAITRACGQEKWWGIEPAVGFAALMAVEGLLARVPG
                     TKAALIAGLLALTAASVWVLRKPGRDDLPGSVFFWVAGLVAALILTIPFAVTGHWGLL
                     GMGYNNDLGLHLAWAEWLRSGFGTEPSDGYPLGPHGLVAALSTIPDFALGRVFIAQVA
                     AIAVLTVMTAWAAVEPLGRWRRLLAAVLVGLPYLMVSYYAQAAFKELATAMFLLAFVI
                     ALPRLTPLPPERRFRAVFPLLVLLLGIIFTYSFPGLAWPAAALLAWLLADPDFRTRLH
                     PRAVWAWLKRPAVWISLVVGLGLVAALAFLGPFGFGDAFSEVAASNAFGPVSVLEGLG
                     IWLTSDYRLAGNLDRWPPDLPEGASWGVGSVVRDSLPIDARLHAVDFSDNYRVLVGRD
                     VAEKGRLRELLAEGMAWSAAAAVFFALMGAAVL"
BASE COUNT          392 a          889 c          864 g          460 t
ORIGIN      
        1 catcgggtgc cggccgatct cgacatcgaa atcggcggca atcgacgctg cgccgtcgag
       61 cgcaaaattg acgtcctcgc gcggcccgaa ttcatgggag acatccgacg gggcgccgct
      121 cgtctcctcc ttggagcgac ggtcgggcac cggctcgtag gcgctcacca cgtcaagcga
      181 ggcaccagac atctgggcaa gcgccgccgc ctgacggatc gcctcccggg ccgaatccga
      241 accatccgta ccgacaacta tgcgattgaa cagggacatt ggcctccgat cgtcgtgggc
      301 ggtcaccggc atcctacccc cggcatcacc cttatctggg aaactcctgc tttggcagac
      361 cctgaaagtc gggcccgaag atttcggttg gcgtggaaag cagctgaatc gtgccgatgg
      421 tcacggaagc gaccaggaag acgcagatca cgatcatcag gattctccag ttggtcggca
      481 tgagtcgcag ggtacgagaa tgagactgaa gatgacatcg gaagccagca catgaacgcg
      541 aaacgggccg tgccaaaggg actcgaagaa gtggccgacg gagtctggct gctgaccggc
      601 gacatcaaga aggcgatgaa catctacttc atcgaaggac cggacgggga gatcgtccag
      661 ttcgatgcgg gaaccaggcc aatgacaaag aggaaccgcc gggcgatcga agaattcggg
      721 ggaatcgacc gggtggtgct cggccacgca cacgccgacc accggggcac ggcccccggc
      781 ctcggcgttc ccgtctggtg ccatcccgac gaggtggccg atgcgacctc gagcgcctcg
      841 atcgccccct acatgaatct cagggaactg gagttcgctc cggtccgctg gatctatccc
      901 ttcctgcttc gccgctggga cggcgggccg gtcgagatcg ccggcaccgt cgaggagggc
      961 gatgaagtcg ccggattcaa ggtgatccac ttccccggcc atgccccggg cctgatcggc
     1021 ctctggcgcg aatcggaccg ggtcgccgtg gtcagcgatg tcgtttactt cgttgattcg
     1081 gcccggctga agccgctacc ggagggcgag gcctcggtac cgcatccggc ctgggcctgg
     1141 gaccatcaga aggccaagga atccgtgcgc aagctggccg cactgaatcc cagggtggta
     1201 gccgccggcc acgagcatcc gctccgtggt gacgacctga gcgacgtgct gctgagagca
     1261 gccgacaagt actgatcagg ggagtcccgg ccgcggatgt tctacagctg gcttcccatg
     1321 ctcctgatcg tggcggggtc cctggtgatc ggccgggcga taacccgcgc ctgcggccag
     1381 gagaaatggt ggggaatcga gccggccgtc gggttcgccg cgctgatggc tgtcgaaggg
     1441 ctgctggccc gggtgccggg caccaaggcg gccctgatcg ccggccttct cgccctgacg
     1501 gcggccagcg tctgggtcct caggaaaccg ggccgggacg acctgcccgg ttcggtcttc
     1561 ttctgggtcg ccggcctggt tgcggcgctg atcctgacca ttcccttcgc ggtgaccggc
     1621 cactgggggc tcctgggcat ggggtacaac aacgatctcg gcctgcatct ggcctgggcc
     1681 gaatggctcc ggtccggctt cggcaccgag ccgagcgacg gctacccgct cggcccccac
     1741 gggctcgtcg cggcactatc cacgatcccc gacttcgccc tcggccgtgt gttcatcgcc
     1801 caggtcgccg cgatcgccgt gctcaccgtg atgaccgcct gggccgcggt cgaaccgctg
     1861 ggacgctggc gccgcctcct tgcggccgtc ctggtcggct tgccgtacct gatggtctcc
     1921 tactacgccc aggcggcctt caaggaactg gccacggcga tgttcctgct tgcgttcgtg
     1981 atcgccctgc cccggctcac ccccttaccg ccagaacgaa ggttccgggc ggtcttcccc
     2041 ctgctggttc tcctgctggg gatcatcttc acctacagct tccccgggct cgcctggccg
     2101 gctgccgccc tgctggcctg gctcctggct gatccggatt tccgtactcg cctccacccc
     2161 cgggccgtct gggcctggct gaagcggccc gcggtctgga tctcgctcgt cgtcggcctc
     2221 gggctggtcg ctgcgctggc cttcctcgga cctttcgggt tcggtgacgc cttctccgag
     2281 gtggccgcca gcaacgcctt cgggcccgtg tcggttctcg agggactcgg catctggctc
     2341 acctccgatt accgcctcgc cggcaatctc gaccgctggc cgcccgacct gcccgagggc
     2401 gcgtcctggg gcgtcggcag cgtggtccgc gacagcctgc cgatcgacgc gcggctgcat
     2461 gcggtggatt tctccgacaa ctaccgtgtg ctggtcggcc gcgacgtggc cgagaagggc
     2521 cgcctgcgcg agcttctcgc cgagggcatg gcgtggtcgg cggccgccgc ggtcttcttc
     2581 gccctgatgg gtgccgcggt gctgc
//
LOCUS       DAOTIP010000267         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_46974_length_2605_cov_2.730196, whole genome shotgun sequence.
ACCESSION   DAOTIP010000267 DAOTIP010000000
VERSION     DAOTIP010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46974_length_2605_cov_2.730196"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="P5138_05900"
     CDS             complement(<1..785)
                     /locus_tag="P5138_05900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRV60143.1"
                     /translation="MTDIPEKTFERFVAIGDSQTEGVGDPTGAGGIERGWADRFAERL
                     AQTQRTYGGGELLYANLAVRGKLLAQIDEDQMEPALAMEPDLVSLVGGLNDVIRPGCD
                     IDLILARMDTMQDRLTSTGATVLTITYPDPALMMPLGKLLSDTMAEFNRGLRRIAERH
                     GTLVLDVAESAEVTDPEHWCDDRLHLNSVGHQVMADGMFSLIEPLPEGQSWLGEITPD
                     RIQGLPARVMAEARWAGAFLAPWIYRRIAGKSSGDGREAKRPEL"
     gene            complement(879..2525)
                     /locus_tag="P5138_05905"
     CDS             complement(879..2525)
                     /locus_tag="P5138_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV60144.1"
                     /translation="MAKKEKKSETVAKPPNLLLIITDQQRYPMHWPEDQEWLDQLMPA
                     DAELARTGVTFEQTCVASCMCSPSRASLFTGRWPAEHGVDLTLTRGGAEVDRSHGRHA
                     IRKGVESARAGEVTPLEMITNVIKGTFRRPNGGTDERELDPHMENLPSVLKRAGYKTV
                     LKGKWHLTQPVGEDWSQADAERLADEYGFEGWVPPDAGENIEPSHFGGGHGSGKSKEG
                     YDEDFTRQAEEFLADPPDEPWALIFSLVNPHDVLGYPSSYEEGGYSSSEWSDLDQVGL
                     PPSVDETLKNKPRAHALLKMGQLGFLGPIEGEQRLEYCRFYAHLHRLADSKISRVLDA
                     LGDPEDPESLRSKTVIFRTSDHGELGMSHGGLRQKMFNAYEETVNVPLIVSNPQMFPE
                     PRRTTAPASLCDVLPTMADFAGVDTTGLELRGHSLVPVLEGKEESVQEGTLFTYDDHQ
                     SGTAYRDVNPSANRIRAWRTPEATYAIYLHPHGGQPEFELYDRTRDPDQVDNLVDRDS
                     GRALNPADRELVEKMREGLKAEMRRCRIPALPAKVERMPV"
BASE COUNT          387 a          853 c          880 g          485 t
ORIGIN      
        1 agttcgggcc tcttggcctc ccggccgtcc cccgaggact tgccggcaat ccggcggtaa
       61 atccaggggg cgaggaaggc gccggcccag cgggcctcgg ccatcacccg ggccggcagg
      121 ccctggatcc ggtcaggggt gatctccccc agccatgact gcccctccgg cagcggttcg
      181 atcaggctga acatgccgtc ggccatcacc tgatgtccga cggagttcag gtggagtcgg
      241 tcgtcgcacc aatgctcggg gtcggtcacc tcggccgact cggcgacgtc gagcaccagg
      301 gtcccgtggc gctcggcgat gcgccggagc ccgcggttga actcggccat ggtgtcggac
      361 aggagcttgc cgaggggcat catcagcgcg ggatccggat aggtgatggt caggacggtg
      421 gctccagttg aggtcagccg gtcctgcatc gtgtccatcc gggcgaggat gaggtcgatg
      481 tcgcagcccg gccggatcac gtcgttgagg ccgccgacca ggctgaccag atccggctcc
      541 atcgccaggg ccggttccat ctggtcctcg tcgatctggg ccagcagctt cccgcgaacg
      601 gccaggttcg cgtagagcag ctcgcctccg ccgtacgttc gctgggtctg ggccaggcgt
      661 tccgcgaacc ggtcggccca gccccgctcg atgccaccgg ccccggtcgg gtcaccgacc
      721 ccctcggtct ggctgtcgcc gatcgcaacg aaacgttcga aggtcttctc cgggatatct
      781 gtcacgccgg aaaccctatc ccccctcgcc cgtcggccgg gaacgaagct gggttcgatt
      841 tacccccgca tatcaggggc aaattgagcc caggctggtc agaccggcat gcgctctacc
      901 ttggcgggca gcgcgggaat gcggcaacgg cgcatctccg ccttcagccc ctcacgcatc
      961 ttctcgacca gttcccggtc ggccggattc agcgccctgc cgctgtcacg gtcgaccagg
     1021 ttgtcgacct gatcgggatc gcgggtccgg tcgtaaagct cgaactcggg ttggccgccg
     1081 tgaggatgga gatagatcgc gtaggtcgct tccggagtgc gccaggcccg gatccggttg
     1141 gcgctcgggt tgacatcccg gtaggcggtt ccggactgat ggtcgtcgta ggtgaagagg
     1201 gtgccttcct gcaccgactc ctctttgcct tccagaacgg gaaccaggct gtggccgcga
     1261 agctccaggc cggtggtatc gactccggcg aaatccgcca tggtcggcag gacgtcacag
     1321 agtgaagccg gggccgtggt ccggcggggc tcgggaaaca tctgcgggtt ggagacgatc
     1381 agcggcacgt tgacggtctc ctcgtaggcg ttgaacattt tctgcctgag gccaccgtgg
     1441 gacatgccta gctcaccgtg atcggaggtg cggaagatca ccgtcttcga gcgcagggat
     1501 tccgggtcct ccgggtcgcc cagggcatcc aggacccgtg agatcttgct gtcggcgagc
     1561 cggtggaggt gtgcgtagaa gcggcagtac tcgaggcgct gctcgccctc gatcggaccg
     1621 aggaagccga gctgccccat cttcagcagc gcgtgagccc gcggcttgtt cttcagggtc
     1681 tcatcgacgc tgggcggaag gccgacctgg tcgagatcgg accactcgct gcttgagtat
     1741 ccgccctcct cgtaggagga cgggtagccg agaacgtcgt gggggttgac cagagagaag
     1801 atcagggccc atggctcgtc gggcgggtcg gcgaggaact cttcggcctg tctggtgaag
     1861 tcctcgtcgt agccttcctt gctcttgccg gaaccgtgac cgccaccgaa atggctcggc
     1921 tcgatgttct cgccggcgtc cggcggcacc cagccttcga acccgtactc gtcggccaga
     1981 cgctcggcat cggcctgcga ccagtcttca ccgaccggct gggtcagatg ccacttgccc
     2041 ttgaggacgg tcttgtatcc ggcccgtttg aggacgctgg gcaggttctc catgtgagga
     2101 tcgagttcac gctcgtcggt gccgccgttc ggccggcgga acgtcccctt gatgacgttg
     2161 gtgatcatct ccagcggtgt cacctcgccc gccctggccg actccacgcc cttgcggatc
     2221 gcgtgccggc cgtggctccg gtcgacctcg gccccgccgc gggtcagggt caggtccact
     2281 ccgtgctcgg ccggccagcg cccggtgaac agcgaggccc ggctcggcga gcacatacag
     2341 gaggcgacgc aggtctgctc gaaggtcacc ccggtgcggg ccagttcggc gtcggccggc
     2401 atcaactggt cgagccactc ctggtcttcg ggccaatgca tcgggtagcg ctgctggtcg
     2461 gtgatgatca gcagcaggtt cggcggcttg gccacggttt cgctcttctt ctccttcttc
     2521 gccatgatca gagacccgct ttcggacaac ctagtttctg gaatgccgac ttgctggtgt
     2581 acgagccgcg ggggtaatag cgacc
//
LOCUS       DAOTIP010000268         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47067_length_2601_cov_3.299293, whole genome shotgun sequence.
ACCESSION   DAOTIP010000268 DAOTIP010000000
VERSION     DAOTIP010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47067_length_2601_cov_3.299293"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..581
                     /gene="arfB"
                     /locus_tag="P5138_05910"
     CDS             168..581
                     /gene="arfB"
                     /locus_tag="P5138_05910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRV60145.1"
                     /translation="MKPGIRPRGGPFIPVSEITLRASRSSGPGGQHANVTASRIEAVF
                     EVRDSLALNDSQKARITRKLGSVVTAVSQDARSQTRNRDLALERLESKLAGALTVPRR
                     RRPTKPTRASKQKRLESKRQRSETKRNRRPPRDDE"
     gene            574..1734
                     /locus_tag="P5138_05915"
     CDS             574..1734
                     /locus_tag="P5138_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRV60146.1"
                     /translation="MSEPVIRILHTADWHLGREFHGRDLTSLHQHFFDWLAAEIESRE
                     IDVVIMAGDIYDRALPPVDAVKMHNRQLPRLAGLADLILVTGNHDSVNRMGFGPMLRE
                     GLHLRSGIEGLGEPVLIERDFQLAIYPIPYLDPMTMANELDLDESTHTAVIEEAVRRC
                     RDDLASRGGARSIAVGHAFITGSETSESERSIQVGGSESVSASVFDGFDYVALGHLHR
                     PQAVGGHIRYAGSPIPLSYSEVGAGAPKSVTVLELSADGSIEQEQVEIPQMVGMARVS
                     ATLEELLNKAEFKDIEGDWLEVTLTDSARPDQPMDRLRSRFENVVSLRFDAPISSASE
                     REARELAEIAEADPARLVSTFLEEVRGSKPTEAEAALVREALDWKSGEETVS"
     gene            1731..>2601
                     /locus_tag="P5138_05920"
     CDS             1731..>2601
                     /locus_tag="P5138_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV60147.1"
                     /translation="MKVHALEITGIGPYAETVEIDFDSLDEVGIYLLTGPTGSGKTTV
                     LDAISYALFGSIPKQAKGHEVVSDHREISTTPRVKLEATIGGERYRITRSPAHEAPKV
                     KGEGTKNEGQSLTVERRDSESWSQLTGAWTEGNDLLEKRVGMNSNQFNQVVLLPQGEF
                     ATFLQAKVGERKDLLMQLFPGADLEWLEKWMKQRAQEDREARDAKLEQIHDCFQAVKT
                     TAESLVDEGEEALPRIGEATPALNWIEDGEGTLAKRHEERRAIRDAAAKEREQADLAV
                     TRLNQKVERIAARK"
BASE COUNT          536 a          832 c          821 g          412 t
ORIGIN      
        1 catcggcttc gcgatgctcg cgctctacat ggcctaccgg ctgctcaagc gcgtcttcgg
       61 aaatgacccg catgaccccg ccgagcccgg aacggaaccg ctcgccgatc cgggtcccga
      121 ttccccggcg ggctccggcg cgtcgacatg aagccgggca tccgaccatg aagccgggca
      181 tccgcccccg tggcgggccg ttcatcccgg tcagcgaaat caccctccgc gcatccagat
      241 cttcaggtcc cgggggccag cacgccaacg tcacagcctc ccggatcgag gccgtcttcg
      301 aggtcaggga ctcgctcgcc ctcaacgact cccagaaggc ccgcatcacc cgcaagcttg
      361 gttccgtggt gaccgcggtc agccaggacg cacgttccca gacccgcaac cgcgacctcg
      421 cccttgagcg cctggaatcg aaactggccg gagccctgac cgtaccccgc cggcgccggc
      481 cgaccaaacc aacccgggcc tcaaagcaga aacgcctcga atcaaaacgc caacggtcag
      541 aaaccaagcg caaccgccga cctccaaggg acgatgagtg aaccggtgat cagaatcctc
      601 cacaccgccg actggcacct cggccgcgaa ttccacggcc gcgacctgac cagccttcac
      661 cagcatttct tcgactggct cgcggccgaa atcgaatccc gcgaaatcga cgtcgtgatc
      721 atggccggcg acatctacga ccgggccctt cccccggtcg acgcggtgaa gatgcacaac
      781 cggcagcttc cgcggctggc cggtctggct gacctgattc tggttactgg caaccacgac
      841 tcggtcaacc gcatgggttt cggcccgatg ctgagggagg ggcttcacct gcgctccgga
      901 atcgaggggc tcggcgaacc ggttctcatc gaacgggatt tccagcttgc gatctatccg
      961 atcccctatc tcgacccgat gaccatggcc aacgaactgg acctggacga atcgactcat
     1021 acggccgtta tcgaggaagc ggtgcgccgt tgccgggacg acctggcgag tcgcggaggg
     1081 gcccgttcga tcgcagtcgg tcatgccttc atcacgggat ccgaaaccag tgagtccgag
     1141 cgcagcattc aggtcggtgg ttcggagagc gtttcggcct cggtcttcga tggatttgat
     1201 tacgtggctc tgggccatct tcaccgtccg caggcggtgg gtgggcacat ccgttacgcc
     1261 ggttcgccga tcccgctctc gtactccgag gtgggcgcgg gcgctccgaa gtcggtgacc
     1321 gtccttgagc tctccgctga tggatcgatc gaacaggaac aggtcgagat cccgcagatg
     1381 gtcgggatgg cccgggtcag cgcaaccctc gaggaactgc tcaacaaggc ggaattcaag
     1441 gacatcgagg gtgactggct cgaggtgaca cttaccgact cggcccgccc cgatcagccc
     1501 atggaccgtc tgcgcagtcg gttcgagaac gtcgtcagtc tgcgctttga cgctccgatt
     1561 tcgagcgctt ccgagaggga ggccagggaa ctcgccgaaa tagccgaagc cgacccggct
     1621 cgtcttgttt ccaccttcct cgaggaagta cgaggaagca agccgaccga ggcggaggcg
     1681 gccctggtcc gggaagccct cgattggaag tccggcgagg agacggtctc ttgaaggttc
     1741 acgccctgga gatcactgga atcggccctt acgccgaaac ggtcgagatc gacttcgaca
     1801 gccttgacga agtcggcatc tatttgctga caggaccgac cggctcgggg aagacgactg
     1861 tgctggacgc gatctcgtat gcgctgttcg gctcgatccc aaagcaagcg aagggccatg
     1921 aagtggtcag cgaccacagg gagatttcga cgacgccaag ggtcaaactt gaagcgacga
     1981 tcggcggcga gcgctatcgg atcacgcgga gtcccgcgca tgaagccccg aaggttaagg
     2041 gcgagggaac gaagaacgaa ggtcagtccc tcaccgttga gcgtcgagac tccgaaagct
     2101 ggtcgcagct gacgggcgcc tggacggaag gcaacgacct gcttgagaaa cgggtcggca
     2161 tgaactccaa tcagttcaac caggtggtgc tgctgccgca gggagagttc gccactttcc
     2221 ttcaagcgaa ggtcggtgag cgcaaggacc tgcttatgca gcttttcccg ggagctgacc
     2281 tggagtggct cgaaaagtgg atgaagcagc gggcgcagga ggaccgggaa gcccgcgacg
     2341 ccaagctcga gcagatccac gactgcttcc aggccgtgaa gacgacagcg gaatcgctcg
     2401 tcgacgaagg ggaagaggca ctcccccgga tcggggaagc cacccccgcc ctgaactgga
     2461 tcgaagacgg cgaagggacc ctggcgaaga ggcacgagga acgaagagcc atccgggacg
     2521 ctgcggcgaa ggagcgggaa caggccgact tggcggtcac ccggctgaat caaaaagtgg
     2581 agcgcatcgc agcacgcaag g
//
LOCUS       DAOTIP010000269         2587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47378_length_2587_cov_3.806872, whole genome shotgun sequence.
ACCESSION   DAOTIP010000269 DAOTIP010000000
VERSION     DAOTIP010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47378_length_2587_cov_3.806872"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..576)
                     /locus_tag="P5138_05925"
     CDS             complement(151..576)
                     /locus_tag="P5138_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60148.1"
                     /translation="MLGGGWLVFGRGGEDSDSSKSATSTTEPAKDTDDWNTKPAKVQG
                     PKLPPSHCPAESSNCVVASGRVIYVEAVDPDGDGDAHFVTVSEQSVTMSGITIFDVRA
                     DLRPDPLPGIGDLVGGAGPVYPGHAGQKQIQVDEFHVAR"
     gene            complement(618..2249)
                     /locus_tag="P5138_05930"
     CDS             complement(618..2249)
                     /locus_tag="P5138_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV60149.1"
                     /translation="MATDTASSAATSSNGSSPASTPPLESFNPATGAVIGSVETITPD
                     QVQGIVDEVAEIQPAWAELRPSDRATYLKRAATVLHDRLDEIASLLSQEQGKPISECY
                     TMELIPSIDALRWCAKEGPGILKDEKISYPQPFFKTKRSKFRYQPIGVVGVIAPWNYP
                     WTIPMQEIAIALMCGNGVVLKPASLTPLLGEAIQSVFEDAGLPKGLVRNVHGGGRIGD
                     ALTKSTAGKIFFTGSVPVGYKVGEECAKLMKGCVLELGGKDPMIVLEDANVDFAISGA
                     VWGGFANAGQTCAGIERAYVRREVSEKFIEGVVEGAKALKVGDPSEVDTEIGPMVSKD
                     QYDLVCELVDDAVASGATLRCGGPVDVEGFPEGNFIAPVVLTDVTHEMRIMKEEIFGP
                     VLPIITVQSDQEALDLANDSEFGLGASVWTKDRQRGERIADRIESGMVWINDHMFTHG
                     ACQCTWGGVKDSGLGHSHSKFGFYECVEIKLVTYEPGLTRNFWWHPYDETLATAMKSS
                     ASLLYGKGGQRVEALKSGAGPLLEVGRRITKRRSR"
BASE COUNT          470 a          834 c          807 g          476 t
ORIGIN      
        1 gtttccggcc ggttcctttc ccggaacaag gcaaagatct atgtgaaggc gagcgagggc
       61 acctgcaagg cccacctgtc gggcaagaga ctcaggtttc agtgatcccg gggcggggct
      121 gaacgccagg cccgacttct ccgtaaacgc tcagcgggcg acgtggaatt cgtcgacctg
      181 gatctgcttc tgcccggcat gcccgggata taccgggccg gcgccgccga ccagatctcc
      241 gatgcccggg agcggatcgg gcctgaggtc ggcccggacg tcgaagattg ttattcccga
      301 catggtcacg ctttgttccg agacggtcac gaaatgggcg tcgccgtcac cgtcgggatc
      361 taccgcttcg acgtagatca cccggccgct ggccacgacg cagttcgacg attcagccgg
      421 acaatgcgaa ggcggcagtt tcggtccctg gaccttcgcc ggctttgtgt tccagtcgtc
      481 ggtgtctttc gccggttcgg tcgtggaggt cgcggacttc gaggaatcgg agtcctcacc
      541 accccggcca aacaccagcc agccgccgcc gagcacgacg acgaccagcc cgatgaaggc
      601 gaggaacttc agcagcccta ccgggagcgg cgtttggtga tccggcgccc gacctcgagc
      661 agcgggccgg ccccggactt gagcgcctcg acccgctggc cgcccttgcc gtagagcagg
      721 ctggccgagg acttcatcgc cgtggcgagg gtctcgtcat aggggtgcca ccagaagttg
      781 cgggtcaggc cgggttcgta ggtgaccagc ttgatctcca cgcattcgta gaagccgaac
      841 ttggaatggg agtggccgag accggaatcc ttgaccccgc cccaggtgca ctgacaggcg
      901 ccgtgggtga acatgtggtc gttgatccag accatgccgg actcgatcct gtcggcgatc
      961 cgctcaccgc gctgacggtc cttggtccag accgaggcgc cgaggccgaa ctccgaatcg
     1021 ttggcgaggt caagcgcctc ctgatcggac tgaacggtga tgatcggcag caccggaccg
     1081 aagatctctt ccttcatgat cctcatctcg tgagtgacgt cggtgaggac caccggggca
     1141 atgaagttgc cctccgggaa gccctcgaca tccaccggac cgccgcagcg caaagtcgcg
     1201 ccgctggcca ccgcgtcgtc gaccagttcg cagaccaggt cgtactggtc cttgctgacc
     1261 atcgggccga tctccgtgtc cacctcggag gggtcgccga ccttgagggc tttcgctccc
     1321 tcaaccacgc cctcgatgaa cttctcgctg acctcacgac gcacataggc gcgctcgatc
     1381 ccggcacagg tctggccggc gttggcaaag ccaccccaga ccgccccgga aatcgcgaaa
     1441 tcgacattcg cgtcctcgag cacgatcatc gggtccttgc cgcccagctc cagtacgcag
     1501 cccttcatca gcttggcgca ttcctcgccg accttgtaac cgaccgggac cgatccggtg
     1561 aagaagatct tgcccgccgt cgatttggtc agggcatcac cgatgcgacc gccgccgtgg
     1621 acgttgcgga ccaggccctt ggggaggccg gcatcctcga acacgctctg gatcgcctcg
     1681 ccgagcagcg gggtgaggct ggccggcttg agcacgacgc cattgccgca catcagggcg
     1741 atcgcgatct cctgcatcgg gatcgtccac gggtagttcc acggagcaat cacgccgacc
     1801 acgccgatcg gctggtacct gaacttcgat cgcttggtct tgaagaacgg ctgcgggtag
     1861 gagatcttct cgtccttgag gatccccggg ccttccttcg cgcaccagcg cagagcgtcg
     1921 atcgaaggga tcaactccat cgtgtagcac tcggagatcg gcttgccctg ctcctgcgag
     1981 agcagcgagg cgatctcgtc gagacggtcg tgaaggaccg tggcagcacg cttcaggtag
     2041 gtcgcacggt ccgacggccg gagttcggcc caggccggct gaatctcggc gacctcgtcg
     2101 acgatcccct ggacctggtc cggagtaatc gtctcgacgc tgccaatcac cgcaccggtg
     2161 gcgggattga acgactccag cggaggagtc gaagcgggcg aggatccgtt tgaactggtc
     2221 gctgcggaag aggcggtgtc ggtagccatg aaccgaagtg tacgccggag aaacaactcg
     2281 gccggagacc cccagtccga gtctccggcc gacacagcac cccacctggc gtcttgctca
     2341 agttgggtct gatcaatgaa accctgaaag cctgaaaaag ttgtgggatt tggctaaagg
     2401 ttccctaagc gttttcggct tgggataacg gatccaaagg cattgccaag ctggcaaaag
     2461 tcagtcgtta tggctggcct tcgccatccc gattcttctg gccccggatc tttgccgcgg
     2521 cttcacggcg gcgggcaagt tccgggacga gatcggtctc atatatctca ccccagttgt
     2581 tatcatt
//
LOCUS       DAOTIP010000270         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47403_length_2586_cov_3.708416, whole genome shotgun sequence.
ACCESSION   DAOTIP010000270 DAOTIP010000000
VERSION     DAOTIP010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47403_length_2586_cov_3.708416"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="P5138_05935"
     CDS             <1..1306
                     /locus_tag="P5138_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60150.1"
                     /translation="IPAAEELRYYNRTRAQYPDLPFTLFFGSIGHPRGQNQANVNAAL
                     KTKEDQWTDYYLGGQGTRPPSEVVSYTQTCPLGGDAGGPYTADDWASLSPGEIRLTDS
                     SAKTIGATGGDSAIAGAWNGVTAGNDPCGTQSGAPEPGSANWDFAAAPAGGYTMQGSP
                     TVIANIDLGDSPNSEIAARLVDLSPGGTVKTLIARTIYRPWPTGVQVFQLHPNAWKVE
                     EGHSLRLELLPKDAKSPAGTFLQNSFRPADGQGDITVSDMDLRIPVVELPGSLGGMVQ
                     SPAKKVLPDRAGVKLAPGYSAIGALTIDSSLKAGSKGTVKGKSLKIMLRCPASYTNQC
                     PNRKVVIKGAPKKGKGKGVKIAAGTNIKVTGGKSRQVTLKLTAKARKLFRDRKKRKVV
                     RRHGRKKVRIVKVKGLKKLRVAVLIDGQRVGYANLRRTGQVR"
     gene            1418..>2586
                     /locus_tag="P5138_05940"
     CDS             1418..>2586
                     /locus_tag="P5138_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRV60151.1"
                     /translation="MRKLAVLVALLGSLFVVSTAQAVPPPASIFDGDVQCGQVTADGN
                     VAGSTGQIWCGTLRNWSGVSGTIDDNGDGSITSTVNPALPSDARATVESFDGVPIDVN
                     VAFPSTGSAPYPVVMMFHGYGGQRFNFKEIQHYLQKGYAVYTQTNRGFHQSCGKPSAI
                     AADADCATKGYIHLDDTRFEVRDAQTIIGELVDEGWVKPDVAAQGGSYGGGMSMALAA
                     LKDRIMLPDGTYDAWESPNGTPMSIAVAAPTIPWTDLAYALNPNGSDIDYIKDAKYQG
                     PFGVMKQSYVNGLYLSGSSNGTYAPEGQDPDADITGWKALILGSLSEMDLCREVAEKA
                     GASARSLAGTTDLSGTAALLGMCRLTLANDSGGMHLSAAVGTPVVAIYGLTDPGKT"
BASE COUNT          495 a          874 c          821 g          396 t
ORIGIN      
        1 gatcccggcc gccgaggaac tccgctacta caaccggacc cgggcccagt acccggatct
       61 tccgttcacc ctcttcttcg gctcaatcgg acatccgcgc ggccagaatc aggccaatgt
      121 caacgctgcc ctgaagacca aagaggatca gtggaccgac tactacctcg gtggccaggg
      181 aacccggccc ccgtccgaag tcgtctcgta cacccagacc tgccccctcg gcggcgacgc
      241 cgggggtcca tatacggccg atgactgggc ctcgctctct cccggggaga tccggctgac
      301 ggactcgtcg gccaagacga tcggggcaac cggcggtgac tccgccatcg cgggcgcctg
      361 gaacggcgtg accgcgggca atgacccatg cggcacccaa tccggcgccc ctgagcccgg
      421 atcggccaac tgggacttcg ccgccgcccc ggcaggcggc tacacgatgc agggctcccc
      481 gacggtgatc gccaacatcg acctcggtga ttccccgaac tcggagattg cggcccgcct
      541 ggtcgacctc agcccgggcg gaaccgtcaa gaccctgatc gcaaggacga tttaccggcc
      601 atggccgacc ggggtccagg tcttccagct ccaccccaat gcctggaagg tcgaggaagg
      661 ccactcgctg cggctcgaac tgctgccgaa ggacgccaag tcaccggccg gcaccttcct
      721 ccagaactcg ttccgacccg ccgacggcca gggcgacatc acggtcagtg acatggacct
      781 gcgcatcccg gtcgtcgaat tgccgggttc gctcgggggt atggtccagt ccccggcgaa
      841 gaaggttctg cccgaccgcg ccggcgtcaa gttggctccc ggctattcgg cgatcggtgc
      901 cctgaccatc gactcctcgc tcaaggccgg gtcgaagggg accgtgaagg gcaagagcct
      961 gaagatcatg ctgcgctgcc cggccagtta caccaaccag tgcccgaacc ggaaggtcgt
     1021 catcaagggc gccccgaaga agggcaaggg caagggagtg aagatcgcgg ccgggacgaa
     1081 catcaaggtc accggcggca agtcgcgcca ggtcaccctg aagctcaccg cgaaggcccg
     1141 caagctgttc cgcgacagga agaagcggaa ggtggttcgc aggcacggcc gcaagaaggt
     1201 caggatcgtc aaggtcaagg gcttgaagaa actcagggtc gcggtgctga tcgatggcca
     1261 gcgggtcggc tacgcgaacc ttcgccgtac cggccaggtt cggtgaacgg aggcagtacc
     1321 cggttctcgc aacttgttgc gtacaggcgc gttctataaa gtgacttcgc cccgggatcg
     1381 gcccgcggca gggaaaacag gagagaaccg gagaatcatg cgcaagcttg cagtgctcgt
     1441 tgcccttctg ggcagccttt tcgtcgtttc gaccgcccag gcagtaccgc ccccggcgtc
     1501 gattttcgat ggcgacgtcc aatgcggcca ggtcaccgcc gacggcaacg tcgccggatc
     1561 gaccggccag atctggtgcg gaaccctccg caactggtcc ggcgtatcag gcacgatcga
     1621 cgacaacggg gacggctcga tcacctcgac tgtcaatccg gcgctcccct ccgacgcacg
     1681 cgccacggtc gaaagcttcg acggcgtccc gattgacgtc aacgtcgcct tcccgtcgac
     1741 cggttcggct ccttacccgg tggtcatgat gttccacggc tacggcggac agcggttcaa
     1801 cttcaaggag atccagcact acctccagaa gggctacgcg gtctacacgc agaccaaccg
     1861 cggtttccat cagtcctgtg gcaagccttc cgcgatcgcc gcggatgcgg actgcgccac
     1921 caagggctac atccacctcg acgacacccg cttcgaggtg cgtgacgcgc agaccatcat
     1981 cggcgaactg gtcgacgaag gctgggtcaa gccggacgtc gctgcccagg gcggcagcta
     2041 tggcggcggc atgtcgatgg cactcgccgc cctgaaggac cggatcatgc tgcccgacgg
     2101 cacctacgac gcctgggaga gcccgaacgg cactccgatg agcatcgccg tggctgcgcc
     2161 gaccatcccg tggaccgacc tggcctacgc gctgaacccg aacggttcgg acatcgacta
     2221 catcaaggat gcgaagtacc agggtccgtt cggagtcatg aagcagtcct atgtcaacgg
     2281 cctgtacctc agcggatcct cgaacggcac ctacgcgccg gagggccagg accccgacgc
     2341 ggacatcacc ggctggaagg ccctgatact gggctcgctc tcggaaatgg atttgtgccg
     2401 ggaagtggcc gagaaggccg gcgcatcggc ccgatcgctg gcgggaacca ccgatctttc
     2461 cggaacggcg gccctgctgg gaatgtgcag gttgaccctg gccaatgaca gcggcggcat
     2521 gcatctgtcg gccgcggtgg ggacgccggt ggtggcgatc tatggactga ccgacccggg
     2581 caagac
//
LOCUS       DAOTIP010000271         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47481_length_2583_cov_3.483782, whole genome shotgun sequence.
ACCESSION   DAOTIP010000271 DAOTIP010000000
VERSION     DAOTIP010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47481_length_2583_cov_3.483782"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1624)
                     /locus_tag="P5138_05945"
     CDS             complement(140..1624)
                     /locus_tag="P5138_05945"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRV60152.1"
                     /translation="MSPEPLWTPTAEAIENSQLTAWARWLKEKGIGPGSEEYPDLWQW
                     STDQPEEFWRSLWDYFEVLHDGEVTEVLSSREMPGAKWFEGTKLNYAEHIFRDRDPEA
                     VAIIGRTESGGWNRDLTWGELEEAVVNARSWLNWNGIEAGDRVAAYMPNIYETIVFFF
                     ATASLGAVWSSCSPDFGAGAVRDRFAQIEPKILIGITDYDYGGKNFDRRATVIELANS
                     MPSVEKVLFLGDGDADCNLPEGVEWDEGRFSTGADLYDAVGDRPDPTGSKLEFTRVPF
                     DSPLWILYSSGTTGLPKPIVQGQGGILLEHLKKMHLHLDAKEGDRIFWFTTTGWMMWN
                     FLTSCLLTDAAIVLYDGNPGFPDMSALWKLASDAEVTCFGTSASYISACMKDGVEPGA
                     DGGDLSRLRSVGSTGSPLAPEGFDWIYEHIGEDTWLFSTSGGTDLCTAFVGGVPTLPV
                     YRGELQARALGAAVESWDEQGHPHIGEVGELVITKPMPSMPLYL"
     gene            complement(1621..>2583)
                     /locus_tag="P5138_05950"
     CDS             complement(1621..>2583)
                     /locus_tag="P5138_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60153.1"
                     /translation="RAARLQEVRIPASESATLALHSAIVIAASLLGLKWPAIGATICL
                     IATFSFYSERGLGLRLIGRAIPGRRTSNVLSPPPGPAWEEVEVILATGYDVPDSYPVG
                     EWLSRRFSGRITTDRFLFYGGMIGTFAALMMRAVGIEDTSLDIFQTLTTAIPMAVIAA
                     QIDRHLAGTPIATQEDLAPARDVMAAAQEADNESEGDSGVAVCLFGAEYAAAGGANTF
                     FSDSRLKLKDGCALVNLVRGARASAPEVTGSEGDLMPTRMSPDLAASSPLKPKTVALR
                     NETAATIARRRGLRATSVVGRGEAGVDLLLDASEGALPDDEAET"
BASE COUNT          435 a          813 c          894 g          441 t
ORIGIN      
        1 cgcgattgat ggtcgagtcg gagcggccgt agatgatcgc ggttccccgc tcggtgatct
       61 cgatccagtc gccgtggcgc cagatgccgg ggtagacgtc gaagtagctg tcgtggtagc
      121 gggagccatc ctcgtcgccc tagaggtaga ggggcatgga aggcatcggt ttggtgatca
      181 ccagttcacc gacctcgccg atatggggat ggccctgctc gtcccatgac tcgaccgcag
      241 cgccgagcgc ccgggcctgg agttcgcccc ggtagaccgg cagggtcggg accccgccga
      301 cgaacgcggt gcagagatcg gtgccgccgg aagtcgaaaa caaccaggtg tcctcgccga
      361 tatgttcgta gatccagtcg aaaccctcgg gggcgagcgg ggagccggtc gatccgaccg
      421 atcggagcct ggagaggtcg cccccgtcgg cgcccggttc gaccccgtcc ttcatgcatg
      481 ccgagatgta tgaggccgag gtgccgaagc aggtcacctc ggcgtcggag gccagcttcc
      541 agagggctga catgtccggg aagccggggt tgccgtcgta gagcacgatc gcggcatcgg
      601 tcagaaggca ggaggtgagg aagttccaca tcatccagcc ggttgtcgtg aaccagaaga
      661 tccggtcgcc ctccttggcg tccaggtgga gatgcatctt cttcaagtgc tcgagcagga
      721 tcccgccctg cccctggacg atcggcttcg gcaggccggt agttccggac gagtaaagga
      781 tccagagcgg agaatcgaag gggacgcggg tgaactcgag cttcgatccc gtggggtcgg
      841 gacggtcgcc gacggcgtcg tagaggtcgg ccccggtcga gaacctgccc tcgtcccact
      901 cgaccccttc gggaaggttg cagtcagcgt cgccgtcacc gaggaacaga accttctcga
      961 ccgatggcat cgagttggcc agttcgatca cggtcgcgcg gcggtcgaag ttcttgccgc
     1021 cgtagtcgta atcagtgatt ccgatcagga tcttcggctc gatctgggcg aagcggtcac
     1081 ggaccgcgcc ggctccgaag tcgggcgagc aggaagacca gaccgcgccg aggctcgcgg
     1141 tggcgaagaa gaagacgatc gtctcgtaga tgttgggcat gtaggcggca acacgatcac
     1201 cggcctcgat gccgttccaa ttcagccagg atcgggcgtt cacgacggcc tcttccagtt
     1261 cgccccaggt caggtctcgg ttccagccgc cgctttcggt gcgtccgatg atcgccacgg
     1321 cctccgggtc ccggtcgcgg aagatgtgct cggcgtagtt gagcttcgtt ccctcgaacc
     1381 acttcgcacc cggcatttcc cggctggaga gcacctcggt cacctcgccg tcgtggagca
     1441 cctcgaagta atcccagagc gagcgccaga actcctccgg ctggtcggtc gaccattgcc
     1501 agaggtccgg gtactcctcg ctgcccggtc cgattccctt ctccttcagc cagcgcgccc
     1561 acgcggtcag ttgcgaattt tcgatcgctt ccgctgtggg ggtccagagc ggctcgggac
     1621 tcaagtctcc gcctcgtcgt cgggcagggc gccttccgag gcgtccagca gcaggtcaac
     1681 gccggcttcg ccccggccga ccacgctggt ggcacggagg ccgcgccgtc tggcgattgt
     1741 ggcagcggtc tcgttccgga gcgcgaccgt cttcggcttc agcgggctgg aggcggccag
     1801 atccgggctc attctggtcg gcatcaggtc accctcgctg ccggtcacct cgggggccga
     1861 ggcccgggcg ccacggacca ggttgaccag ggcacaaccg tccttcagct tcaggcggga
     1921 atcggagaag aaggtgttgg ccccgccggc cgcggcgtac tcggcgccga aaaggcagac
     1981 tgcgactccg gaatcacctt cggattcgtt gtcggcctcc tgcgcggcgg ccatcacgtc
     2041 acgggccgga gcgagatctt cctgtgtggc aatcggagtc ccggccagat gccggtcgat
     2101 ctgggcggca atgaccgcca tcggaatcgc cgtcgtcagg gtctggaaga tgtccagaga
     2161 ggtgtcctcg atgccgaccg ctctcatcat cagggcggcg aaggtgccga tcatgccgcc
     2221 gtagaagagg aaccggtcgg tcgtgatccg tcccgaaaag cggcgggaga gccattcgcc
     2281 gaccgggtac gagtcgggga cgtcgtaacc cgtagcgagg atcacctcaa cttcttccca
     2341 ggccgggccc gggggagggg agagcacgtt gctggttcgg cgtccgggga tggcccggcc
     2401 gatcagccgc agaccgaggc cgcgctcgga atagaacgag aaggtggcga tcaggcagat
     2461 cgtggccccg atcgccggcc atttcagccc gagcagactg gcagcgatga cgatcgccga
     2521 atgcagggcg agggtggctg attcggaggc ggggatgcgg acttcctgca gccgggcggc
     2581 gcg
//
LOCUS       DAOTIP010000272         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47700_length_2573_cov_3.267673, whole genome shotgun sequence.
ACCESSION   DAOTIP010000272 DAOTIP010000000
VERSION     DAOTIP010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47700_length_2573_cov_3.267673"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..864)
                     /locus_tag="P5138_05955"
     CDS             complement(172..864)
                     /locus_tag="P5138_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60154.1"
                     /translation="MPRFILTLGLLLGALSAALVVSGCGGDDDGQFANEATGEFPVQI
                     IDANFKKLQTVAQTYDLTIAVRNSGDETIPAINTVIDLPGKGSTLAFAYGDKQPGLAM
                     NQRPVWVVEEGWPKLADTVARGGATTPDRRTFEFGKLKPGDTANMVWRVVAVRPGNYL
                     LKYQISAGLGPDTSAVDANGNVPTGVLPVHITDFARLTKVNAKGQVVPVSRAEQAHVE
                     AQQESANTGLNP"
     gene            1001..1807
                     /locus_tag="P5138_05960"
     CDS             1001..1807
                     /locus_tag="P5138_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60155.1"
                     /translation="MVKDTAQKAIEPAKEFFTETGEMMILTGRTILSAVKPPYPYGNE
                     FIGQFLFALQLCWFPMLISTVAFGFGAPGLQAANFLSLFGALDRLGGFFVLASIREFA
                     PFVTAVVVAGVAGTAITADLGARKIREELDALQVLGVDPIKNLVVPRFLALMLITGML
                     DIYALLFGIAGGIGAELLYSQPLGGFFASLFSNASTTELAASIIKCTLFGAIIAIVCC
                     YKGMTATGGAAGVGRAVNEAVVTALLGVFIFNYLFTQTMLATNPDIQTIK"
     gene            1807..>2573
                     /locus_tag="P5138_05965"
     CDS             1807..>2573
                     /locus_tag="P5138_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60156.1"
                     /translation="MAGSWLQVPREWLESIGEIARFCGAVMGLVFSGRVFKYFGEVLN
                     QAGILILGSTTVIWGLVFVLGLQCGIEGAYFNRSVGAPAYAGVFAAWCDLREVIPYAF
                     GYMMSAKVGTGIVAELGAMRISDEIDALEVMGVPPVTFLAATRLLGAWITLPFMYIAA
                     IGVGYLASYIAVVEQIGDVSSGGYQLIFWMFQNPPDVLFSLIKAMTMSTAIVLVGCYY
                     GYTASGGPVGVGTATAKSMVLNIVLVHIIGMLGTLVF"
BASE COUNT          399 a          851 c          812 g          511 t
ORIGIN      
        1 ggactggtca ctgccgccgg cgcaggcgag cgacactgac gagagggcga aggttgcgag
       61 aaccgcggcg gtggcgcagg aaagacggcc catggggatc actgataccc gaaaccggcc
      121 ccggccttca agttgcgcat tggcaggcgg gcccggaggc cgtcggccgc gttacgggtt
      181 caggccggta ttggcggatt cctgctgggc ctcgacgtgg gcctgttcgg cccggctcac
      241 cgggacgacc tggcccttgg cgttgacctt ggtcaggcgg gcgaaatcgg tgatgtgaac
      301 cggcagcact ccggtcggga cgtttccgtt ggcgtcgacc gcgctcgtat ccggcccgag
      361 accggccgag atctggtact tcagcaggta gttgccgggc cgaacggcga caacccgcca
      421 gaccatgttg gcggtgtcgc ccggcttcag ctttccgaac tcgaaggtcc tccggtcggg
      481 cgtcgttgcc ccgccgcggg cgaccgtgtc agccagtttg ggccagcctt cctcgaccac
      541 ccacaccggg cgctggttca tggccagtcc cggctgcttg tcgccgtagg cgaaggccag
      601 ggtcgaaccc ttgccgggca gatcgatcac tgtgttgatc gccggaatcg tttcgtcgcc
      661 cgagttcctg acggcgatcg tcaggtcata ggtctgggcc accgtctgca gcttcttgaa
      721 attcgcatcg atgatctgga ccgggaattc accggtcgcc tcgtttgcga actggccgtc
      781 gtcatcaccg ccgcagccgg agacgacaag ggcggcggag agcgcgccca gaagcagtcc
      841 gagggtgaga atgaatctcg gcatggatct caggctggtc cccctcaagg tcgggcgcaa
      901 cataccacgc gtgtggcgac ggtcacacac gccggggtat cgcatagact ccgcgccacg
      961 gcgggtcacg ggaagtccac ggggccggcg cccgggagag atggtcaaag acaccgcaca
     1021 aaaggcgatc gagcccgcga aggaattctt caccgaaacc ggtgaaatga tgatcctgac
     1081 cggacgcacg atcctgtccg cggtcaagcc tccctatccc tacggaaacg agttcatcgg
     1141 gcagttcctc ttcgcccttc aactctgctg gttcccgatg ctgatctcaa ccgtcgcctt
     1201 cggcttcggc gccccgggac tccaggccgc caacttcctt tcgctcttcg gagccctcga
     1261 ccgcctcggc ggttttttcg ttctcgcttc gatccgtgag ttcgcgccgt tcgtgaccgc
     1321 ggtcgtggtc gccggcgtgg ccggcaccgc gatcaccgcc gatctcggcg cccgcaagat
     1381 ccgcgaggag ctggacgcgc tccaggtcct cggcgtcgat ccgatcaaga acctggtcgt
     1441 gccgcgcttc ctggctttga tgctgatcac cggcatgctc gacatctacg ccctgctctt
     1501 cggcatcgcc ggcggcatcg gtgccgagct gctctactcg cagcccctgg gcggcttctt
     1561 cgcttcgctc ttctcgaacg cgtcgaccac cgagctcgcc gcctcgatca tcaagtgcac
     1621 cctgttcggg gcgatcatcg cgatcgtctg ctgttacaag ggcatgaccg ccaccggtgg
     1681 tgcggccgga gtcggccggg ccgtcaacga ggcggtcgtc accgccctgc tcggcgtctt
     1741 catcttcaac tacctgttca cgcagaccat gctggcgacc aaccccgaca tccagacgat
     1801 caagtgatgg ccggcagctg gctacaggtc cctcgcgagt ggctcgaatc aatcggcgag
     1861 atcgcccgct tctgtggcgc ggtcatgggt ctggtcttct ccggacgggt tttcaagtac
     1921 ttcggcgagg tcctcaacca ggccggcatc ctgatcctcg gttcgaccac ggtgatctgg
     1981 ggcctggtct tcgtgctcgg cctgcagtgc ggcatcgaag gggcgtactt caaccgctcg
     2041 gtcggcgcac ctgcctacgc aggcgtcttc gccgcctggt gcgacctccg cgaggtcatc
     2101 ccctacgcat tcggctacat gatgtcggcc aaggttggca ccggcatcgt cgccgagctc
     2161 ggcgcgatgc ggatctcgga tgagatcgac gcgctcgagg tgatgggcgt gccaccggtc
     2221 accttcctcg ctgccacccg gctgctcggg gcctggatca ccctgccttt catgtacatc
     2281 gcggcgatcg gtgtcggcta cctcgcctcc tacatcgccg tggtcgagca gatcggcgac
     2341 gtttcttccg gtggctatca gctgatcttc tggatgttcc agaacccccc ggatgtctta
     2401 ttcagcctga tcaaggcaat gacgatgtca accgccatcg tcctggtcgg ctgttactac
     2461 ggctacacgg cctcgggcgg accggtcggc gtgggcaccg cgacggcgaa atcgatggtt
     2521 ctgaacattg tgctggtgca catcatcggt atgttgggga ctctggtttt ctg
//
LOCUS       DAOTIP010000273         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47927_length_2563_cov_3.516746, whole genome shotgun sequence.
ACCESSION   DAOTIP010000273 DAOTIP010000000
VERSION     DAOTIP010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47927_length_2563_cov_3.516746"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="P5138_05970"
     CDS             complement(<1..817)
                     /locus_tag="P5138_05970"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRV60157.1"
                     /translation="MTLPAPTRHLTAFGLALAALLLMASSLASRSEAATYQEGTVEVN
                     GYNWKVDPVSVDLNDIAPTDDGATAYTVLQILEKADNSQYDIDTVPGVTINLPGKLTG
                     PGCTGDQVRAESNQCPTFTSTADATTMRYRKGSQVKTVKYTSYNPQIYIDRASDMKVE
                     LSPPSKKIKSGDSVKFTTEVTGAIGQVTYTWDFGDGSQKTTSKGSISHTFTGSNRTYT
                     VVVDVSSTGNPRGDDDISTITVGKVKKTTKKTDKNQNQNDSGGGTGGTGGTSSG"
     gene            complement(814..1836)
                     /locus_tag="P5138_05975"
     CDS             complement(814..1836)
                     /locus_tag="P5138_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HRV60158.1"
                     /translation="MKSVKYGKPARINLKGGVRLAHGGRSVAVRNLRVVSAPGKAALL
                     KGKLGKRTVTFLRSKGGKRKYNARRGTLVQTGGARLGRGAANLINRRLRLKGPQKLRT
                     GTYWGGFNLYSLYKVTPVEDPTGEVPEIPPDKTEPQDAKTVASAATIKWYVRESFIDY
                     IASGEGTRVEDGATADPASGSNNLVYSFNFPFDTGWTVPESGSGPENTLIKGTGLVGF
                     RFCQNTINFTAANPEIEIDGDDNSRLIFEVNGTDGTAFPDQRAVMFKLIPSRAQSHTV
                     TDNGNGYSTSTYVKIPGYVPAEGTGIFAGLYPAYSPDNDSLPQDQRPDRFGYFSVTYT
                     FPNVDH"
     gene            complement(2149..>2563)
                     /locus_tag="P5138_05980"
     CDS             complement(2149..>2563)
                     /locus_tag="P5138_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HRV60159.1"
                     /translation="NFPVGSGEYAANEGGITSDDQAIINGSGTGLICNNEHHFWVSIS
                     DPTVVIDGTDSRIVATISQNMNGGGIMGPVGPWQTPQRVDLADLDLTGVTPTYGTGTV
                     TWSDIPVTLSSDAAPFATYAAGQSLDPITVSLETE"
BASE COUNT          366 a          775 c          891 g          531 t
ORIGIN      
        1 taccggacga ggtgccgccc gtcccgcccg tgccgccgcc ggaatcgttc tggttctggt
       61 tcttgtccgt cttcttggtc gtcttcttca ccttgcccac ggtgatcgtc gagatgtcgt
      121 cgtcgccgcg ggggttgccc gtagaggaga catcaaccac aacggtgtag gtgcgattgc
      181 tgccggtgaa ggtgtgggag atcgatcctt tggaggtcgt tttctggctg ccgtctccga
      241 agtcccatgt gtaggtgacc tggccgatcg cgccggtcac ctcggtcgtg aacttgaccg
      301 agtcgccgct cttgatcttc ttgctgggcg gcgagagttc gaccttcatg tcgctggccc
      361 ggtcaatgta gatctgcggg ttgtagctgg tgtacttcac cgtcttgacc tgggagccct
      421 tgcggtagcg catggtggtc gcgtcagcgg tcgaggtgaa ggtcgggcac tggttcgact
      481 cggcccggac ctggtcaccg gtgcagccgg ggccggtcag cttgcccggc aggttgatgg
      541 tgactccggg aacggtgtcg atgtcgtact ggctgttgtc ggccttttcg aggatttgca
      601 ggaccgtgta cgcagtcgcg ccatcgtcgg tcggggctat gtcattcagg tcgaccgaga
      661 ccgggtccac cttccagttg tatccgttga cctcgactgt cccctcctgg tacgtcgctg
      721 cttccgaccg cgaagcgaga gaagacgcca tcagcagcaa tgcggccagg gccaggccga
      781 aagccgtgag gtgtcgtgtc ggtgcgggaa gggtcaatgg tcgacgttgg ggaaggtgta
      841 ggtgaccgag aagtatccga agcggtcggg cctctggtcc tggggcaggc tgtcgttgtc
      901 ggggctgtag gccgggtaga ggccggcgaa aataccggtg ccctcggccg ggacgtagcc
      961 gggaatcttc acataggtgc tggtgctgta gccgttgccg ttgtcggtca cggtgtggga
     1021 ctgggcccgg ctcgggatca gcttgaacat caccgcccgc tggtcgggga aggcggtgcc
     1081 gtcggttccg ttgacctcga agatcaggcg cgagttgtcg tcaccgtcga tctcgatctc
     1141 cgggttggcg gcggtgaagt tgatcgtgtt ctggcagaac cggaagccga ccaggccggt
     1201 gcctttgatc agcgtgttct ccgggccgct cccggattcg ggcacggtcc agccggtgtc
     1261 gaaggggaag ttgaacgagt agacgaggtt gttggatccg ctcgccgggt cggcggtcgc
     1321 gccgtcctcg acccgggttc cttcgccgct ggcgatgtaa tcgatgaagc tttcacgcac
     1381 gtaccacttg atcgttgccg ccgacgcaac agtcttcgcg tcctgcggtt cggtcttgtc
     1441 cggagggatt tccggcacct caccggtcgg atcttcgacc ggtgtcacct tgtagagcga
     1501 gtagaggttg aagccgcccc agtaagtccc ggtgcgcagc ttctgcgggc ccttgaggcg
     1561 gagcctgcgg ttgatcaggt ttgccgcgcc ccgaccgagc cgcgcgccac cggtctggac
     1621 cagggtgccc cgcctggcgt tgtacttccg cttgccgccc ttgctccgaa ggaaggtgac
     1681 ggtccgcttg ccgagcttgc ccttgagcag ggcggctttg cccggggcgg agacaaccct
     1741 caggttgcgg accgcgacgg agcgtcctcc gtgtgcgagc ctgactcctc ccttgaggtt
     1801 gattctggcc ggcttgccgt acttcactga cttcaccggg aaggtcggcc ggccggtaaa
     1861 cccgctcgcc ggtttgatca gcttctgacg gaccccgctg cgcttccacg ccttccgggt
     1921 cgcccggccg gggctcaggg tgagctcgcc gtcgaggtcg atgatcggcc cggtcggctg
     1981 ggttgactgg cagggctcga gggttgccgc cccggccggg gccggtccgg ccccaagccc
     2041 gacgaaaacg gcgagcagaa ccgcgacgag ggcgaacccg ggaagggtcg ccctcgtcgt
     2101 tgccagatga ctggcggaat atgaggcccg gtgcctcagc cgggtcccct actcggtctc
     2161 cagcgagacg gtgatcgggt cgagtgactg acccgccgcg taggtcgcaa acggagcggc
     2221 atcgctgctg agcgtgaccg gaatgtcgct ccatgtgacc gtgccggtcc cgtaggtggg
     2281 ggtgacaccg gtcaggtcca ggtcggccag gtcaacacgc tggggggtct gccagggccc
     2341 gaccggaccc atgatcccgc caccgttcat gttctggctg atcgtggcga cgatccggga
     2401 atcggtcccg tcgatcacga ccgtcggatc gctgatcgac acccagaaat ggtgctcgtt
     2461 gttgcagatg aggccggtgc ccgaaccgtt gatgatcgcc tggtcgtccg aggtgatgcc
     2521 accttcgttc gccgcatact caccgctgcc caccgggaag ttg
//
LOCUS       DAOTIP010000274         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_47981_length_2561_cov_3.879489, whole genome shotgun sequence.
ACCESSION   DAOTIP010000274 DAOTIP010000000
VERSION     DAOTIP010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47981_length_2561_cov_3.879489"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1153
                     /locus_tag="P5138_05985"
     CDS             20..1153
                     /locus_tag="P5138_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60160.1"
                     /translation="MFDTEQDDYRESFRQFLDKEVVPKYPEWERNHIVPKSLFTSCAE
                     YGFLAMEVPEEYGGPGVEDWRFNVVLAEESIWAGVGPAMGGPLLHTDIVVPYITSTAT
                     EEQKKRWLPGVASGEKVLAVAMTEPGTGSDLAAISTRGKRDGDGWVVNGAKTFITNGI
                     NADMVVVAVRTSDDPHGGLSLFVIEEGMEGFDRGKQIEKLGQHASDTAELFFNDCYVP
                     QENMLGEEGSGFLQLVSRLVPERLSLSVSSMAGCEAALAMTIDYAKERKAFGRPIANF
                     QHSRFTLAELRTEVEIGRCFIDRCIERYVEGTCTVEEAAMAKWWTTDLLSKVTDAGVQ
                     LHGGYGYTTEYQIGKAWVDARVGRIYAGTNEIMKELIGKTMGL"
     gene            complement(1193..1792)
                     /locus_tag="P5138_05990"
     CDS             complement(1193..1792)
                     /locus_tag="P5138_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRV60161.1"
                     /translation="MPTTRRQPKTQAQRRAETKAALLDATVESLVTYGYAGTTTGRIA
                     ELAGVSRGAQTPYFRSRADLISAAVTHLSELRIAAVRDRFSGKNVSIEEGLDALWEEH
                     QGDAFDALLELWIASRTDDEMREHLITIERSVGVAIVRESAAALGPIADRPDFNDDMI
                     YILATMRGLSIMRISHGVSDQALARRWEEIRHRLAKILS"
     gene            complement(1830..>2561)
                     /locus_tag="P5138_05995"
     CDS             complement(1830..>2561)
                     /locus_tag="P5138_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRV60162.1"
                     /translation="RQTVELGRASRAQAKAKVRQPLARAVVVATDAEREAISSQAELI
                     KSELNVKELEFVTDQSDLVSYLVKPNFRTLGPRFGKAMPQVSAAVAGLDPAHVAETVE
                     AGGEIGISIEGKDHSLTPEDLLLSMEPLEGYQVEAESGHAVALALELDEDLIREGLAR
                     EIVHAVQNARKDAGLEITDRISLSLAGDQELLDAAATHEGYVTGEVLARSIELGGDTS
                     AAAAPGTPEVTIDGRTLAISVARVD"
BASE COUNT          442 a          824 c          849 g          446 t
ORIGIN      
        1 ggtagtcggc aagcgaacca ttttcgacac cgagcaggac gactatcggg agagcttccg
       61 ccagttcctc gataaggaag tcgtgccgaa gtatccggag tgggaacgga accacatcgt
      121 ccccaagagc cttttcacca gttgcgccga gtacggcttc ctggccatgg aggttcccga
      181 ggagtacggc ggcccggggg ttgaggactg gcgcttcaac gtcgtgctgg ccgaggaatc
      241 gatctgggcg ggggttggcc cggcgatggg tgggcccctg ctgcacaccg acatcgtcgt
      301 gccctacatc acctcgaccg ccaccgagga acagaagaag cggtggctgc ccggagtcgc
      361 atccggtgag aaggtcctgg ccgtcgcgat gaccgaaccc ggaaccgggt ccgacctggc
      421 ggcgatctcg acccgtggca agcgtgacgg cgacggctgg gttgtcaacg gcgccaagac
      481 cttcatcacc aacggcatca acgccgacat ggtcgtggtc gcagttcgca cctccgatga
      541 cccccacggc ggcctctcgc tgttcgtgat cgaggagggc atggaagggt tcgaccgggg
      601 caagcagatc gaaaagctcg gtcagcatgc ctccgacacg gccgagctct tcttcaacga
      661 ctgctacgtg ccgcaggaga acatgctcgg cgaggaaggc tccggcttcc ttcaactggt
      721 ttcgcgcctg gttccggaac ggctcagcct ttcggtctcg tcgatggccg gttgcgaagc
      781 ggcgctggcg atgaccatcg actacgccaa ggaacgcaag gcattcggtc ggccgatcgc
      841 gaacttccag cacagccgct tcaccctggc cgaacttcgc accgaggtcg agatcggccg
      901 ttgcttcatc gaccgctgca tcgagcggta cgtcgaaggg acctgcacgg tcgaggaggc
      961 ggcgatggcg aagtggtgga ccaccgacct gctcagcaag gtcaccgacg cgggcgtgca
     1021 gctgcacggc gggtacggat acacgaccga gtaccagatc ggcaaggcct gggtggacgc
     1081 cagggtaggc cggatctacg cgggcaccaa cgaaatcatg aaggaactga tcggaaagac
     1141 gatggggctc tgagagttcg ccggcagccg ccggtccccg accccggggg tctcaggaaa
     1201 ggatcttcgc cagccggtgc cggatctctt cccagcgtct ggccagagcc tggtcgctga
     1261 ccccgtgact gatccgcatt atcgacaacc cgcgcatcgt ggcgaggatg taaatcatgt
     1321 cgtcgttgaa atcggggcgg tcggctatcg ggcccagggc tgccgcagat tccctgacga
     1381 tcgccacccc gacgctcctt tcgatcgtga tcaggtgctc gcgcatttcg tcgtcggtcc
     1441 gcgacgcgat ccacaattcg agcagcgcgt caaaggcatc gccctgatgt tcctcccata
     1501 gcgcgtcaag gccctcttca atcgacacgt tcttgccgct gaaccggtcc ctcaccgcag
     1561 cgatccgcag ttcggacaga tgggttactg cagcgctgat cagatctgcc ctgcttcgga
     1621 agtagggggt ctgggcacca cgcgaaacgc cggcaagttc ggcgatccgg cccgtagtcg
     1681 tgcctgcata gccgtaggtg accagcgatt caaccgtggc atcgagcagg gcagccttgg
     1741 tctcggcgcg gcgctgagcc tgggtctttg gctggcgacg ggtggttggc atgggctgaa
     1801 gatatctgca gggccgctgc tgccctgtct cagtcgaccc gggcgaccga gatggcgagc
     1861 gtccggccgt cgatagtgac ctcgggggtg ccgggcgccg cggccgcgga agtgtcaccg
     1921 ccgagctcga tcgagcgggc cagcacctcg ccggtcacgt agccctcgtg ggtcgccgcg
     1981 gcatccagga gctcctggtc accggccagc gaaaggctga tccggtccgt gatctcgagc
     2041 cctgcatcct tgcgggcgtt ctggaccgcg tggacgatct cgcgggcgag gccctcgcgg
     2101 atcaggtcct cgtccagctc cagggcgagc gcgacggcat gtcccgattc ggcctccacc
     2161 tgataaccct cgagcggttc catgctgagc agcaggtcct cgggggtcag gctgtggtcc
     2221 ttcccctcga tcgagatgcc gatttcaccg ccggcctcga ccgtctcggc cacatgagcc
     2281 gggtcgagcc ccgcaactgc ggcggagacc tggggcatcg ccttgccgaa gcgggggccg
     2341 agggtgcgga agttcggctt gaccagataa ctgaccaggt ccgactggtc ggtcacgaat
     2401 tcaagttcct tgacgttcag ctccgacttg atcagctcgg cctgtgagga gatcgcctcc
     2461 cgctcggcgt cggtcgcgac caccaccgcc cgggccagtg gctggcggac cttggccttg
     2521 gcctgggccc gggaagcgcg tcccagctca accgtctggc g
//
LOCUS       DAOTIP010000275         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48066_length_2558_cov_2.689573, whole genome shotgun sequence.
ACCESSION   DAOTIP010000275 DAOTIP010000000
VERSION     DAOTIP010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48066_length_2558_cov_2.689573"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="P5138_06000"
     CDS             complement(<1..894)
                     /locus_tag="P5138_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60163.1"
                     /translation="MKRLVAATLLMLVASPSLASASSPPVSDWVQTDRVEFHGTDGFF
                     RSQGVASDGSSLFFSWNLGLIRTPVDEPSNVLVDKSTSAIPDDLDESGHNHIGDIDVA
                     GGVLYAPIEDGPAYAEPWVVLYDTDTLEPTGARYLLPVSVQRDGVPWIAVDQPRGVAY
                     SMEWNDTGKLFVYRLSDFELIDTVNLSEPVSRIQGAKIFRGNLYASRDNGSEKSIVSI
                     DPETGQVTRLFDRDLGDDYEAEGIAFIRRRTGTEMVATGIREGSSGYTEMRTYRIGGD
                     VTAPVLRGVHFKSKRTRRGSKL"
     gene            complement(904..2112)
                     /locus_tag="P5138_06005"
     CDS             complement(904..2112)
                     /locus_tag="P5138_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60164.1"
                     /translation="MLVRAGVVMAALAGLGAGNAVAEASPAPVTNWVQTGNVEFSGST
                     ALFRSQGLTSDGSSIYFSWNRGMSSTPVDDTDNVLVDNSTHAIPTDLLDSGHDHIGDI
                     DYANGKLYAPIEDKTYAEPYVVVYDAATLQPTGERHLLPDGSQHHIAWIAIDEPRGVA
                     YSMEWTDTGKLFVYSLDDFSLVRTVTLSSPESRIQGAEVYKGNLYASQDNGGTQSVLA
                     IDPVTGQVTSLFDRNLGDEYESEGIGMVERRSGTMMLATDVLDGSGGFTEMRIYRING
                     DETPPALKSVSFKPKKVRRPKKLTLRATVSEPVSATLRWMKCKGSRKKPCAKKNAVGK
                     TRKVNLSAGANRIKLPPSYPRSGKKPVRLKAGTWQLMVTPTDQADIVGKSKGATVTFL
                     ELRRNGSPKP"
     gene            2168..>2558
                     /locus_tag="P5138_06010"
     CDS             2168..>2558
                     /locus_tag="P5138_06010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV60165.1"
                     /translation="MAVPAAFFDLDKTLMAGSSGMQFAKAARERGLISRAQVLRWGRD
                     HIRYRIKGATDDETGEILKVARETLSGVSYNEISRMWPEVLAGILPRIYPEVLSEVHR
                     HQDEGRLTYIVSAAGSDMVESLAQVLGM"
BASE COUNT          415 a          801 c          841 g          501 t
ORIGIN      
        1 cagcttcgag cccctgcggg tccgcttcga cttgaagtgg accccgcgca ggaccggggc
       61 ggtcacgtcg ccgccgatcc ggtaggtccg catttctgtg tagccgctgc tgccctccct
      121 gatcccggtg gcgaccatct cggtgccggt cctcctgcgg atgaaggcga tgccctccgc
      181 ttcgtagtcg tcgccgaggt cccggtcgaa gagccgggtg acctgtcccg tctcggggtc
      241 gatcgaaacg atcgacttct cgctgccgtt gtcccttgag gcgtagagat ttccacggaa
      301 gatcttcgcc ccctggatgc gggacaccgg ctcgctgagg ttcacggtgt cgatcagttc
      361 gaagtcgctc agccggtaga cgaagagctt gccggtgtcg ttccattcca tcgagtaggc
      421 gacgccgcgg ggttggtcca ccgcgatcca cgggacgccg tcacgctgga ccgagacggg
      481 aaggaggtag cgggcaccgg tcggctcgag cgtgtcggtg tcgtagagca ccacccaggg
      541 ctcggcatag gcgggaccat cctcgatcgg cgcgtagagg acaccgccgg cgacgtcgat
      601 gtcaccgatg tggttgtgcc cgctctcgtc caggtcatcc gggatcgcgg aggtggactt
      661 gtcgacaagc acgttcgacg gctcgtcgac cggggtccgg atcagtccga gattccagga
      721 aaagaagagc gaactcccgt cgctggccac tccctgggag cggaagaacc cgtcggtgcc
      781 gtgaaactcg acccggtcgg tctgaaccca gtcactgacc ggcggcgagg atgcggacgc
      841 cagggacggg ctggcgacca gcatcaacaa cgtggctgct acgagtctct tcatccggga
      901 cggctagggc tttggcgagc cgttacggcg aagttcgaga aaggtcactg tggccccctt
      961 cgacttgccg acgatgtcgg cctgatcggt cggtgtgacc atcagctgcc aggtacccgc
     1021 cttcaaccgg acgggcttct tgccggaccg cgggtagctg ggaggcaatt tgatccggtt
     1081 cgcaccggcc gagaggttca ccttccgggt cttgccgacg gcgttcttct tcgcacaggg
     1141 cttcttcctg gatcctttgc acttcatcca gcggagagtc gccgagaccg gttccgagac
     1201 cgtcgcccgg agggtcagct tcttcgggcg ccggaccttt ttcggcttga aagacaccga
     1261 cttgagggcc gggggagttt cgtccccgtt gatccgatag atccgcattt ccgtgaagcc
     1321 gccgctgcca tcgagtacgt cggtcgcgag catcatcgtc ccggagcggc gctcgaccat
     1381 gccgatgccc tcggactcgt actcgtcgcc gagattgcgg tcgaagaggc tggtcacctg
     1441 gccggtaacc gggtcgatcg cgagaaccga ctgggtgccg ccgttgtcct gcgaggcgta
     1501 gagattgccc ttgtacacct cggcaccctg gatgcgtgat tcgggggagc tcagggtgac
     1561 ggtcctgacc aggctgaaat cgtcgaggct gtagacgaag agcttgccgg tatcagtcca
     1621 ttccatcgag tacgcgacgc cgcgcggttc gtcgatcgcg atccaggcaa tgtggtgctg
     1681 tgacccgtcc ggaagcaggt gccgctcgcc ggtgggctgg agagtcgcgg cgtcatagac
     1741 gacgacatac ggttcggcgt aggtcttgtc ctcgatcggt gcgtagagct tgccgttcgc
     1801 gtagtcgatg tcaccgatgt ggtcgtgccc gctgtccagg aggtcggtag ggatcgcgtg
     1861 ggtgctgttg tccaccagca cgttgtccgt gtcatcgacc ggggtgctgc tcatgccgcg
     1921 attccaggag aagtagatcg aagagccgtc gctggtcagc ccctgggaac ggaacagggc
     1981 agttgagccg ctgaactcga cgttcccggt ctgaacccaa tttgtgaccg gagccggact
     2041 ggcttcggcg actgcgtttc cggctccaag tcccgccagg gctgccatca cgacgccggc
     2101 gcgcacgagc atccgtctca tcattcatcc cctctcctga gatccctcgc cggtaactta
     2161 ccgtcttgtg gcagttcccg cggcattttt cgacctcgac aagaccctga tggcgggttc
     2221 ttccggcatg cagttcgcca aggcggcccg ggaacggggg ctgatctccc gggcccaggt
     2281 cctgcgctgg ggccgcgatc acattcgtta ccggatcaag ggcgcgacag acgacgagac
     2341 cggcgagatt ctcaaggtcg ccagggaaac cctttccggg gtttcctaca acgaaatctc
     2401 ccggatgtgg ccggaagtcc tggccggcat cctgccccgc atctacccgg aggtcctttc
     2461 cgaggtccac cgtcatcagg acgaaggcag gctcacctac atcgtcagcg ccgcgggctc
     2521 ggacatggtc gagtcgctgg cccaggttct gggcatgg
//
LOCUS       DAOTIP010000276         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48081_length_2557_cov_3.790568, whole genome shotgun sequence.
ACCESSION   DAOTIP010000276 DAOTIP010000000
VERSION     DAOTIP010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48081_length_2557_cov_3.790568"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="P5138_06015"
     CDS             <1..730
                     /locus_tag="P5138_06015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV60166.1"
                     /translation="IGLGPEKPATPPSFKRSDGSTGHVERAGVVVQETPLEDGVPLVV
                     QVSRWDKLKDHEGLLTLFARDMKNREVHLALVGPDSSGVDDDPEGAAVYQGILEQFEN
                     LAPDVRSRVHLVSLPMDDLEENAFMVNAIQRRADLIVQKSLAEGFGLTVSEGMWKARP
                     MVASRIGGIQDQIRDGESGILIDDPRDYPAFAAAIDSLLADPERSRAMGQAAQEQVKS
                     GLLSVQRLTLHYRLVDQLISVLRS"
     gene            778..1668
                     /locus_tag="P5138_06020"
     CDS             778..1668
                     /locus_tag="P5138_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV60167.1"
                     /translation="MPAIELHNLKKSFGPINAVDGLDLVVEEGVCLGLLGPNGAGKST
                     TMKMLTGQAIPDAGEIRVLGYKLPREAKQARAEMGVVPQLDNLDIDVTVEENLAVFAR
                     LYRVDDVPAAVERALEIARLTDRRKEAVDRLSGGMRRRLLLARGLVHEPRLLLLDEPT
                     VGLDPQIRTELWTLIDGLRSRGTTILMSTHYIEEAERLADEVAVVADGRVIARATPKE
                     LIAEHAGEKTAEVYGPPDRLHEVRSAAEDAGLRVRSAGPAIAIVGAEMAGPGLVPHDA
                     IHRAGSLEDVFVMLTGEEAE"
     gene            1665..2459
                     /locus_tag="P5138_06025"
     CDS             1665..2459
                     /locus_tag="P5138_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60168.1"
                     /translation="MKSAEGARTVRRLEPAVILAVMGRELANFRTFWKSTAFTSTFEP
                     IVYLLAFGLGLGSTLVADVDGIDYVQFVGTGLVATAVVFSSALPAMFGTFVKERFQRT
                     YDAILAAPVDVEELVTAEMLWIGLRSAIFGCFPLIVTMFFGLEPEPTMLLVPIFAFVT
                     ALAFSAFGITMAASVSKIDQFNYVTTLVITPLFLVAGTFFPIDQLPQGFQIAAQFNPL
                     YQLVQLVRDACFGLEPVDALRFLGLLILAFVLWRIAIRRMTRRLII"
BASE COUNT          437 a          828 c          825 g          467 t
ORIGIN      
        1 gatcggcctc ggcccggaga agccggcaac accgccatcc ttcaagcgat ccgacggcag
       61 caccggccat gtcgagagag caggcgtcgt cgtgcaggag actccgctcg aagatggcgt
      121 tccgctggtg gtgcaggtgt cacgctggga caagctcaag gaccatgagg ggctgctcac
      181 cctgtttgcc cgcgacatga agaaccggga agtccacctt gccctggtcg gccccgacag
      241 ctccggggtt gatgacgatc ccgagggagc ggccgtatat caggggattc tcgaacagtt
      301 cgagaatctg gcccccgacg tccgctcacg ggtgcatctg gtgagcctgc cgatggatga
      361 cctggaggag aacgcgttca tggtcaacgc gattcagcgc cgcgccgacc tgatcgtcca
      421 gaagagcctt gccgaaggat tcggcctcac ggtgtccgag gggatgtgga aggcccgtcc
      481 gatggtcgcc agccgtatcg gcggcatcca ggaccagatc cgtgacggcg agtccggcat
      541 cttgatcgac gacccccgcg attaccccgc gttcgccgcc gcgatcgact ctctgctggc
      601 agatcccgag cgatctaggg caatgggcca ggccgctcag gagcaggtca agtcggggct
      661 gctgtcggtc cagcgcctga ctcttcatta ccggctggtc gaccagctga tttctgtcct
      721 gcgctcttga ggcccggacc gttccggccg ccgcgccggg tctgattgac tgcccggatg
      781 cccgcgatcg aactccacaa cctgaagaag agcttcggac cgatcaacgc ggttgatggc
      841 ctcgatcttg tggtcgagga gggcgtctgc cttggccttc tcggacccaa cggggccggc
      901 aaatcgacca cgatgaagat gctcacaggc caggccattc ctgacgccgg ggaaatccgg
      961 gtcctcggct acaagctgcc gcgggaagcg aagcaggcgc gggcggaaat gggtgtcgtt
     1021 ccccagctcg acaacctcga catcgacgtc accgtagaag agaacctggc cgtctttgcc
     1081 cggctctacc gggtggacga cgtgcccgca gcggtcgagc gggcgctgga aatcgcccgg
     1141 ctgaccgacc gccgcaagga agccgtcgat cgactctcgg gaggcatgcg caggcgcctc
     1201 ctgctcgctc gcggactggt tcacgaaccc agactcctgc tgctcgacga accgacggtg
     1261 ggactcgatc cccagatcag gaccgaactc tggaccctga tcgacggcct caggtcccgc
     1321 ggcaccacga tcctgatgtc gacccactac atagaagagg ccgagcggct ggccgacgag
     1381 gtggcggtgg tggcggacgg cagggtgatc gccagggcca ctccgaagga gctgatcgcc
     1441 gagcatgccg gggaaaagac ggccgaggtg tacggacctc ccgaccgcct gcacgaggtc
     1501 cgctcggcgg ccgaggacgc cgggctcagg gtgcgctcgg ccggaccggc catcgcgata
     1561 gtgggggccg aaatggccgg gccgggactg gtcccccatg acgcgataca ccgggccgga
     1621 tcgctcgagg acgtattcgt gatgctgacc ggcgaggaag ccgaatgaag tccgccgaag
     1681 gcgcgaggac agtccggcga ctggagccgg ccgtgatcct ggcagtgatg ggtcgggagc
     1741 tggcaaattt ccggaccttc tggaagtcaa ccgcgttcac cagcactttc gagccgatcg
     1801 tctatctgct ggccttcggt ctcggactcg gttcgaccct ggtcgccgac gtggacggaa
     1861 tcgactacgt gcagttcgtc ggcaccgggc tggttgccac tgccgtggtc ttctcgtcgg
     1921 ccctgccggc gatgttcggt accttcgtga aagaacggtt ccagcggaca tatgacgcca
     1981 tcctcgccgc cccggtcgac gtcgaggaac tggtcaccgc ggagatgctc tggatcgggc
     2041 tgagatccgc gatcttcggt tgtttcccgt tgattgtcac catgttcttc gggctggagc
     2101 cggagccgac catgctgctg gtgccgatct tcgccttcgt caccgccctt gccttctctg
     2161 ccttcgggat caccatggcg gcttccgtgt cgaagatcga ccagttcaac tacgtgacca
     2221 ccctggtcat cactcccctg ttcctggtcg cgggaacgtt cttcccgatc gaccagcttc
     2281 cgcagggatt ccagatcgcc gcccagttca acccgctcta ccagctagtg caactggtcc
     2341 gcgatgcctg cttcggactc gaaccggtcg atgccctgag gttcctcgga cttctgatcc
     2401 tggccttcgt gctctggcgg atcgcgatcc ggcggatgac gcgccggctg atcatctgag
     2461 ccgtctggtc aggcttcgat gatctcgggc gcgtaaccct ggaatagccc ggactcgagc
     2521 acgccgacga tcttctttat ctcgccttcg aggccgt
//
LOCUS       DAOTIP010000277         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48224_length_2551_cov_3.110176, whole genome shotgun sequence.
ACCESSION   DAOTIP010000277 DAOTIP010000000
VERSION     DAOTIP010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48224_length_2551_cov_3.110176"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="P5138_06030"
     CDS             <1..1459
                     /locus_tag="P5138_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRV60169.1"
                     /translation="GSDDVTTLLGTGSGSFGAATSYPAGTDPRALALFHFDNGASLDL
                     AVANGTSPGNVSAMSGDLSGAFGAPVNNTVGAYSRSIEAADFNNDGILDLVTANGGGN
                     NVSVLLGTGVGTFGAATDFATGTAPNDVAVGDLNGDGRPDLVTANAGASVVSVLLATG
                     PGTFGPKTDYAVASSSLSVAIGDLDNDGALDIAVGTSSASGGTLLPGVGNGTFGTGTS
                     LDLATTYAVAVADLNQDGAKDIVGAAGSANVAEVLINAPDPSAGPGPLVFGNPTPIPA
                     GLVSPPELLPIENNGVAPMSVHGFSFGGPQPQDFFIGGDNCRGVLFEGDQCVARVFFS
                     PKVSGVRAASLTVLTNGESNPVVALVGTGGSLPQGPTGASGPQGPTGPAGKYASINKV
                     TVSGPSSSRKGKSATYTIKIANNGNSKATGVGVKATGKGVSASKGLGAVASGSTGTIK
                     LKIKFKKSGKPKVSFKVTSDNAGGKTVKKTVNVKK"
     gene            1515..2528
                     /locus_tag="P5138_06035"
     CDS             1515..2528
                     /locus_tag="P5138_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRV60170.1"
                     /translation="MEEGGGQGAARERSRRRWVSAICWTLAGFFMLWAILRIGGLERG
                     YPLVQMVAYTPYVLMLSLLALLVVVLCRRWLAAGFLAIAVIALALAVLPRAIGGPESM
                     PGARPVRILTFNLAKSKAETRAVVEEAARREVDLIFLQELTRKKARELNRAGLGSSYP
                     HQVMSFSEKYGNGIWSRWPLIRRESLPVKEQPRADVRVPDSIPIEVVSAHPTAPIHRS
                     DMGLWERDYGVFPPAFSAGIPIVLAGDFNATLDHANLRNVIDTGYRDAAEVIGDGLAP
                     TWPSTIQFPPPVTIDHVLAEKGIAFSQYDVVKIPGSDHKAIFTEILLPPVAGENGSAA
                     GET"
BASE COUNT          472 a          791 c          853 g          435 t
ORIGIN      
        1 gggctcggat gacgtcacca ccctcctcgg gaccggttcc ggatccttcg gggccgcgac
       61 cagctacccg gcagggacgg acccgagggc cctggccctg tttcacttcg acaacggtgc
      121 cagcctcgac ctcgcagtag cgaacgggac gagtccgggg aacgtttcgg ctatgtctgg
      181 cgatctatcc ggcgccttcg gagccccggt caacaacacg gttggtgcgt actccaggtc
      241 gatcgaggcc gcggacttca ataacgatgg aattcttgac ctggtcaccg cgaacggagg
      301 tggcaacaat gtctcggtgc tgctcggaac cggggtcggg accttcggtg cggcaacgga
      361 tttcgccacc ggcaccgcgc ccaacgacgt ggcggtcggg gatctgaacg gtgatggaag
      421 gccggacctc gtgaccgcaa acgccggcgc ttcagttgta tccgtcttgc ttgctacggg
      481 gcccgggacc ttcgggccca agaccgacta cgcggtggcg tcgagctctc tctcggttgc
      541 gatcggcgac cttgacaacg atggcgcgct ggacatcgca gtgggaacct ccagcgcatc
      601 cggaggtact ctcctgccgg gtgtggggaa cgggactttc gggacgggta ccagcctgga
      661 tctggccact acctacgccg tggccgtggc cgacctcaat caggatggag cgaaggacat
      721 agtcggagcg gccggttccg caaacgtggc cgaggtgctg atcaacgcgc ccgatccttc
      781 cgctggaccg ggaccgctgg ttttcggcaa tcccaccccg ataccggcgg gcctggtgag
      841 ccccccggaa ctgcttccaa tagaaaacaa cggtgttgcc ccgatgtcgg tccatggatt
      901 cagtttcgga ggcccccagc cgcaggactt cttcatcgga ggcgataact gccgaggagt
      961 tctcttcgag ggagatcaat gcgtcgcccg ggtcttcttc agcccaaagg tatccggagt
     1021 cagagcggcg agcctcacgg ttctcacgaa cggtgaatcg aatccggtcg tggctctggt
     1081 cggaaccgga ggctccctgc cgcagggccc gaccggggct tcgggtccac agggcccgac
     1141 tggccccgct ggcaaatacg catcgatcaa caaggtgacg gtcagcggac cctccagctc
     1201 gagaaagggc aagagcgcca cctacacaat caagatcgcc aacaacggca actcgaaggc
     1261 gaccggggtc ggtgtcaagg cgactggcaa gggagtcagc gccagcaagg gtctgggagc
     1321 ggtcgcctcc ggttcaacgg ggacgatcaa gctgaagatc aagttcaaga agtccggaaa
     1381 gcccaaggtc agcttcaagg tgacctcgga taacgccgga ggcaagaccg tcaagaagac
     1441 ggtcaacgtc aagaaataga ccaggacccg agcggttcac atgagttccc aggcggggat
     1501 ggataatccc cggcttggaa gaaggcggcg gacagggagc agcgcgggag aggtcccggc
     1561 ggagatgggt atccgcgatc tgctggacgc tggccggatt cttcatgctc tgggcaatcc
     1621 tgcggatcgg gggcctcgaa cggggttacc cgctggtgca gatggtcgcc tacacgccgt
     1681 atgtgctgat gctctccctg ctggcgctcc tggtcgtggt gctgtgccgg cgctggctcg
     1741 cggccggctt cctcgccatt gcggtcatcg cactggcgct ggcggtgctg ccccgcgcga
     1801 tcggtggccc ggaatcgatg cccggcgcgc ggccggtccg catcctgacc ttcaacctgg
     1861 ccaagtcgaa agccgaaacg cgggcggtgg tcgaggaagc ggccaggcgg gaagtcgacc
     1921 tgatcttcct gcaggagctg accaggaaga aggcccggga gctgaaccgg gccggactcg
     1981 gttcctcgta tccgcatcag gtgatgagct tcagcgagaa gtacggaaac ggaatctggt
     2041 cccgctggcc gctgattcgt cgcgaatctc tcccggtaaa ggaacagccg agagccgatg
     2101 tgcgggtgcc ggactcgatc ccgatcgaag tggtttcggc ccacccgacc gccccgatcc
     2161 accgttcgga catgggactg tgggagcgtg actacggcgt cttcccgccg gccttcagcg
     2221 ccgggattcc gatcgtcttg gccggcgatt tcaatgcgac cctcgatcac gcgaacctcc
     2281 ggaacgtgat cgacacgggc taccgtgatg cggcggaagt gatcggcgac ggccttgcgc
     2341 cgacctggcc ttcgacgatc cagtttccgc cgccggtcac gatcgaccac gtgctcgccg
     2401 agaagggaat cgccttttcg cagtacgacg tggtgaagat ccccgggtcg gaccacaagg
     2461 cgatcttcac cgagatcctt ctgccgcctg tggcgggaga gaacggatcc gcggcgggag
     2521 agacctgatt caggcgcggc gcggttccgg g
//
LOCUS       DAOTIP010000278         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48369_length_2545_cov_3.971486, whole genome shotgun sequence.
ACCESSION   DAOTIP010000278 DAOTIP010000000
VERSION     DAOTIP010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48369_length_2545_cov_3.971486"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="P5138_06040"
     CDS             complement(<1..178)
                     /locus_tag="P5138_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV60171.1"
                     /translation="MRADARRNREKVLVAARELFARDGEGVQIEEIANHAGVGIGTVY
                     RHFPTKGALLTEMVR"
     gene            244..1767
                     /locus_tag="P5138_06045"
     CDS             244..1767
                     /locus_tag="P5138_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60172.1"
                     /translation="MEQEFRFRFKPTSNLQELLLSGPDLSTHRAKNLALMLLALTQFV
                     VVIDASITNVALPSIGKALDISQDSLSWVVNSYTLVFGGFLLLGGRLADFLGRRRMFM
                     IGMSLFGLASLAGGLAQSEAWLIIARACQGLGAAIASPAALSIVTTTFAEGSERNRAL
                     GIWGAVAGAGGAAGVLLGGVLTEWAGWEWVLFVNVPIAAFVVWQAPQRLLESREEDES
                     ERTLDIPGAVSITAGLALLVYGLVDADKSGWTSTATLLRIAGSLALIAAFVVIELRTR
                     RPLVPFSIFRMRTLRGANIVGILTGMSLFSMFFLITLYLQQVLNMSALDAGLAYLPLA
                     IAIILAAGMAGQLVTRVGFKPVMTAGLLFVAVALGWFTQISPDGSYVADVLGPSILAG
                     IGLGLTFVPVTIAAMSGSKREEAGLASGLINTSQQVGGALGLAILASIADSTTSSSIA
                     DGSNQAVALTDGFSAAFLVGACFALLGAILAATLISSRDSREHAEAAQAGEVEMPAA"
     gene            1839..>2545
                     /locus_tag="P5138_06050"
     CDS             1839..>2545
                     /locus_tag="P5138_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60173.1"
                     /translation="MNLRLLMVLAAAQFLMVLDQAVMNVSISQLVADFDTTVTTIQAI
                     IAFYALVMAGLMLIGGKLGDIYGRKRIFKIGLVVYASGSALTAASWNVASLAFGWSLL
                     EGIGAAMVLPAMVALVAGNFDGKNRAVAYGVLGGMAGVGIAVGPILGGWMTTDFTWRL
                     VFVGEVILAGAIFIGTRWMLEPAPEGRRPKLDVIGGILSALGLAVMVLGVLQASNWGW
                     LRPSNSPVEIFGFSLTP"
BASE COUNT          350 a          897 c          804 g          494 t
ORIGIN      
        1 tcctgaccat ctcggtcagc agggccccct tggtggggaa gtggcggtaa acggtcccga
       61 tcccgacccc ggcgtggttt gcgatctcct cgatctgcac accctctccg tcacgggcga
      121 agagttcgcg ggcggcgacc agcacctttt cccggttccg gcgggcgtct gccctcattg
      181 ttcgttcggc tggagtcatc tggttgacaa acggaatcgt gtttccgtat agtaccgaaa
      241 taagtggaac aggaattccg cttccgattc aaaccgacca gcaatcttca ggagctcctc
      301 ttgtccggac ccgacctcag cacgcatcgc gccaagaacc tggcgctgat gttgctggcc
      361 ctcacccagt tcgtcgtcgt gatcgacgcc tccatcacca acgtggccct gccctcgatc
      421 ggcaaggcgc tcgacatctc ccaggacagc ctttcctggg tggtcaacag ctacaccctc
      481 gttttcgggg gcttcctgct gctcggcgga cgcctcgccg acttcctcgg ccggcggcga
      541 atgttcatga tcggcatgtc gctcttcggc ctcgcctccc tggctggcgg cctggcccag
      601 tccgaggcat ggctgatcat cgcccgggcc tgccagggtc ttggcgcggc tatcgcctcg
      661 cccgcagcgc tctcgatcgt caccaccacc ttcgccgaag gatccgagcg caaccgggcg
      721 ctcggaatct ggggcgcggt cgccggggct ggcggtgcgg ccggagtgct gctcggcggc
      781 gtgctgaccg agtgggccgg ctgggaatgg gtgctcttcg tcaacgtgcc gatcgccgcc
      841 ttcgtggtct ggcaggcccc gcagcggctg ctcgaatccc gggaggagga cgagtccgaa
      901 cgcaccctgg acatccccgg cgcggtctcg atcacggccg gtctcgccct gctggtctac
      961 gggctggtcg atgccgacaa gtccggctgg acctccaccg ccaccctcct gcggatcgcg
     1021 ggatcgctgg ccctgatcgc cgccttcgtg gtgatcgaac tgcggacccg tcgcccgctg
     1081 gtgccgttct cgatcttccg gatgcggacc ctgagagggg caaacatcgt cgggatcctg
     1141 accggcatgt cgctcttctc gatgttcttc ctgatcaccc tctacctgca gcaggtgctg
     1201 aacatgtcgg cgctcgacgc cggccttgcc tacctgcccc tggcgatagc gatcatcctc
     1261 gccgccggca tggccgggca actggtcacc cgggtcggct tcaagccggt catgaccgcc
     1321 ggccttctgt tcgtcgccgt cgctctcggc tggttcaccc agatcagccc ggacggctct
     1381 tacgttgccg acgtgctcgg tccctcgatt ctggccggca tcggcctcgg gctgaccttc
     1441 gtgccggtga cgatcgccgc gatgtccggt tcgaagcgcg aggaggccgg tctggcttcc
     1501 ggcctgatca acacctccca gcaggtcggc ggcgcgctgg gcctggcgat cctcgcctcg
     1561 atcgccgact cgaccaccag cagctcgatt gcggatggct ccaatcaggc cgtcgccctg
     1621 accgacggct tctccgccgc cttcctggtc ggcgcctgct tcgccctgct gggcgcgatc
     1681 ctcgctgcca ccctgatctc gagcagggac agccgcgagc acgccgaggc cgcccaggcc
     1741 ggtgaagtgg agatgcctgc agcctgaatc gccctgacgc cccgggtcca ccagcatcga
     1801 gtggacccgg ggccgtccgg ccacggataa tcaccggcat gaacctgcga ctgctcatgg
     1861 tccttgccgc cgcccagttc ctgatggtgc tggaccaggc ggtgatgaac gtctcgatct
     1921 cgcaactggt cgcggatttc gacaccaccg tcaccaccat tcaggcgatc atcgccttct
     1981 acgcgctggt gatggccgga ctgatgctga tcggcggcaa gctcggggac atctacggcc
     2041 gcaagcggat attcaagatc ggcctcgtcg tctacgccag cggttcagcc ctgaccgccg
     2101 cttcctggaa cgtcgcctcc ctcgccttcg gctggtcgct cctcgagggg atcggggcgg
     2161 cgatggtgct gccggcaatg gtggcgctgg tcgccggaaa cttcgacggc aagaaccggg
     2221 cggtcgccta cggagtgctc ggcgggatgg ccggcgtcgg aatcgcggtc ggcccgatcc
     2281 tcgggggctg gatgaccacc gacttcacct ggcgcctggt cttcgtcggt gaagtcatcc
     2341 tggcgggggc gatcttcatc ggcacccgct ggatgctcga gccggccccc gagggcagac
     2401 gcccgaagct ggacgtgatc gggggcatcc tttcggcgct cggcctcgcc gtcatggttc
     2461 tcggcgtcct tcaggccagc aactggggct ggctcaggcc gtcgaactcc ccggtcgaga
     2521 tcttcggctt ctcgctcacg ccttt
//
LOCUS       DAOTIP010000279         2540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48497_length_2540_cov_3.459960, whole genome shotgun sequence.
ACCESSION   DAOTIP010000279 DAOTIP010000000
VERSION     DAOTIP010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48497_length_2540_cov_3.459960"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="P5138_06055"
     CDS             <1..773
                     /locus_tag="P5138_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HRV60174.1"
                     /translation="KRTLSESEVGFEPPAEDEMGARLAAVLGVIYLIFNEGYSATAGE
                     EWMRTDLSAEALRLGRILAGLAPGEPEVHGLVALMEIQASRFPARIGPDGAPVLLLEQ
                     DRSLWDRLLIRRGLERLELARGLDGKGPYVLQASIAACHARAATPGETNWEEIVALYD
                     ELATIRDSPVAELNRAVAISMAGDPAAALEAIDRLVDDRRMAGYHLLPSARGDALERL
                     GRHEEAAAEFERASTLTANLQEKRVLDERAANSRKSSA"
     gene            855..1280
                     /locus_tag="P5138_06060"
     CDS             855..1280
                     /locus_tag="P5138_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRV60175.1"
                     /translation="MRCMVFVKATEDSEAGVMPSEKDLAEMGAYNEELVNAGVMLDAA
                     GLHASKKGVRVTYPEGTPTVTDGPFAETKELIAGYWIWEVKSLDEAIEWSRRIPFNHG
                     ESIEIRPFMEPEDFGEALTPELQERGEALEARMEEQKDS"
     gene            complement(1351..>2540)
                     /locus_tag="P5138_06065"
     CDS             complement(1351..>2540)
                     /locus_tag="P5138_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV60176.1"
                     /translation="AGAGASTKKTKQQKKKKKKKAKKGPKLGPAGLKFYKPPKTMPKS
                     GHGKLIWQRKAGGAVPLKSAASTKLVLYTSKSISGKTVAVSGSVSIPKGKAPKGGWPV
                     VTYGHGTTGIADKCAPSRNTKGGIADAYISYTDPQMNQWLRAGYAVVRSDYQGLGTPG
                     DHPFLVGIPEGRSVLDIVTASRQLGMHISNKYLIAGHSQGGHAALWAAGLASTYSKGN
                     KLKGTVAYAPASNFKFMAQALGAYTSPNSFSALATLLVRGMSVAYPDIDTSQLLSDEF
                     LPLYPKTRTDCLSQLAQPDSLGGLAPADLIRVGYDRTRMYEILDAQNPAVKTAAPILL
                     AQGTADATVPKLLTDMLEPKLVEQGDKVDYKLYEGVDHGSVVAAAQSDVMSFFEKRLP
                     GGK"
BASE COUNT          463 a          766 c          850 g          461 t
ORIGIN      
        1 ccaagcgaac gctgtcggaa tcagaggtgg gcttcgagcc ccctgccgaa gacgagatgg
       61 gggcaaggct ggccgcggtg cttggtgtga tctacctgat cttcaacgag ggatattcgg
      121 caaccgccgg cgaggaatgg atgcgaaccg acctttccgc ggaagctttg aggctgggtc
      181 ggatcctggc cggcctggcc cccggggaac ccgaagttca cggccttgtt gccctgatgg
      241 agatccaggc ttcgcgattc ccggcccgca tcggacccga cggcgcgccc gtacttctgc
      301 tcgaacagga ccgctccctg tgggaccggc tacttatccg ccggggactg gagcggctcg
      361 agctcgcaag gggtctggac ggcaagggtc catatgtgct ccaggcttcg atcgcagcct
      421 gtcatgcccg tgcggcaacc ccgggggaaa cgaactggga ggagatcgtt gccctgtacg
      481 acgaactggc gacgatcaga gactctccgg tcgccgaact caaccgggcg gtcgcgatct
      541 cgatggccgg cgaccctgcc gctgcactgg aggcgattga ccggcttgtc gacgacaggc
      601 ggatggccgg gtatcacctg ctgcccagcg caaggggaga cgcactcgaa aggctcggcc
      661 gccacgagga agcagcggct gaattcgaac gcgcctccac gctcaccgca aacctccagg
      721 aaaagcgggt attggacgaa agggcggcga attcaagaaa atcttcggcc tgatgtcgat
      781 ttggccgctc cccgttcgtc ttgtatgcaa atggccggtg acaagggcca caaccgcaac
      841 caaaggagat tgagatgcgc tgcatggtgt tcgtcaaggc aaccgaggat tccgaagccg
      901 gggtgatgcc ttccgaaaag gacctcgccg aaatgggggc atacaacgag gagctcgtca
      961 acgccggggt gatgctggac gccgccggtc tccacgcgag caagaaaggg gtgcgggtga
     1021 cctacccgga gggcaccccg accgtcaccg acgggccctt cgccgaaacc aaggaactga
     1081 ttgccggcta ctggatctgg gaggtgaagt cactcgacga ggcgatcgag tggtcacgac
     1141 gcatcccgtt caatcacggc gagtcaattg agatcaggcc gttcatggag ccggaggact
     1201 tcggcgaggc gctcactccg gagctgcagg agcggggcga ggccctcgaa gcccggatgg
     1261 aggaacagaa ggactcctga accggaatcg ggtaccgaaa cgaagaaggg gacccgcggg
     1321 tccccttctt tcgttccgtt ctggagacgc ctacttgccg ccgggcagac gtttctcgaa
     1381 gaaggacatg acatcgctct gcgcggccgc cacgacgctg ccgtggtcga cgccctcgta
     1441 gagcttgtag tccaccttgt cgccctgctc gaccagcttc ggctcaagca tgtcggtcag
     1501 gagcttcggc acggtcgcat cggccgtacc ctgggccagc agaatcggtg cggcggtctt
     1561 gaccgcgggg ttctgggcgt ccaggatctc gtacatacga gtccggtcgt agccgacccg
     1621 gatcaggtcg gccggcgcga gtccacccag actgtccggc tgcgcgagct gcgaaaggca
     1681 gtcggtcctg gtcttggggt acaggggcag gaactcgtcc gagagcaact gactcgtgtc
     1741 gatatcgggg taggcgaccg acatgccgcg aaccagcagc gttgccaggg cgctgaacga
     1801 attcggcgag gtgtaagcac ccagggcctg agccatgaac ttgaagttcg aggccggggc
     1861 ataggcgacg gttcccttca gcttgttgcc cttgctgtag gtcgaggcga gaccagccgc
     1921 ccagagcgcc gcgtggccac cctgtgaatg accggcaatc agatacttgt tgctgatgtg
     1981 catgccgagc tgcctcgacg cagtgacgat gtcgaggacg ctccgtccct cggggatgcc
     2041 gacgaggaag ggatgatcac ccggggtgcc gagtccctgg tagtcggacc tgacgaccgc
     2101 gtatccggcg cgcagccact gattcatctg cgggtcggtg tacgagatgt aggcatcggc
     2161 gatgccgccc ttggtgttgc gtgaaggggc acacttgtcc gcgatacccg tggtgccgtg
     2221 gccgtaggtg acgaccggcc agccgccctt gggggccttg cccttcggga tgctcaccga
     2281 gccggaaaca gcgacggtct tgccggagat gctcttcgag gtgtagagga ccagcttggt
     2341 agatgcggcg ctcttcagcg gtaccgcgcc acccgccttg cgctgccaga tcagcttgcc
     2401 gtgacctgac tttggcatgg tcttcggcgg cttgtagaac ttgagtccgg ccggtccgag
     2461 cttcggaccc ttcttggcct tcttcttctt tttcttcttc tgctgcttgg tcttcttggt
     2521 ggaagctccg gctccggccg
//
LOCUS       DAOTIP010000280         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48586_length_2537_cov_3.369460, whole genome shotgun sequence.
ACCESSION   DAOTIP010000280 DAOTIP010000000
VERSION     DAOTIP010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48586_length_2537_cov_3.369460"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="P5138_06070"
     CDS             <1..211
                     /locus_tag="P5138_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60177.1"
                     /translation="FDPDRVDCPVRFIWGTSDQLLRWPETAVRFQEKWFPDAVYVELD
                     GVGHCPQLEVPAETAEMIAGTARLA"
     gene            complement(232..921)
                     /locus_tag="P5138_06075"
     CDS             complement(232..921)
                     /locus_tag="P5138_06075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60178.1"
                     /translation="MAKATSVFEKQAATYRGPREKLVPQMDRFYGMVPEAVRLASAAA
                     GSGEGPLRVLDLGAGTGMLSAVVSESFPSASFTLFDFSPRMLEQARELLGEGSVVVSG
                     DMYEGIPAGPWDAVISALAIHHMTDEGKRSVYESAFRELGPGGVFVNAEHVLGETPAL
                     QEHYARWHRWQAYEKGLTDAEWADTVERMSHDHLTPLSTQLGWLREIGFTDVDCLWKD
                     HGFAVFFGRRS"
     gene            complement(966..1589)
                     /locus_tag="P5138_06080"
     CDS             complement(966..1589)
                     /locus_tag="P5138_06080"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRV60179.1"
                     /translation="MGANTDREELIAALSAEVTDRRVLDAIASVPRELFVPEDQRPYA
                     WENRPLPIGQGQTISQPLVVATMCEMLAPGPDDLVLDIGGGSGYHAAVLARLARRVIS
                     IERHPELVGQARQSLEDAGVENVEMICGDGSHGYPAEAPFDVINVAAAARGEVPAALK
                     EQLAEGGRMIVPVGRWRQWLELWRRKDGELSRERVVAVAFVPLVEDG"
     gene            1653..2114
                     /locus_tag="P5138_06085"
     CDS             1653..2114
                     /locus_tag="P5138_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRV60180.1"
                     /translation="MESSAQDQPLEPASHMTGECMCGKVTFEVDAPLIGAALCYCGRC
                     QHRTGTAFSATALTKPGSFRITSGEEHIGSYEPGDGGFNKFFCRECGGQLYTSHPQNP
                     DILAIRMGALNEDPGIRPAAHQFTDYAAAWYPVPDDGLPRFPERIDWTQLL"
     gene            complement(2118..>2537)
                     /locus_tag="P5138_06090"
     CDS             complement(2118..>2537)
                     /locus_tag="P5138_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRV60181.1"
                     /translation="DAPPEVIWDCFMDPELLKQWLGPDRLEMRVDEFDARPGGKWSYV
                     HIDSDGTEYRFFGEFVELDRPVRAVQTFNFVMEPQPPPSVDELNLIPLENDQTRLVTK
                     TTFESREQRDGMIQAGMEKGMNEGYEKLDSILAGRER"
BASE COUNT          424 a          910 c          772 g          431 t
ORIGIN      
        1 gttcgatccc gaccgcgtcg actgcccggt gcggttcatc tggggcacct cggaccagtt
       61 gctgcgctgg ccggagaccg ctgtccggtt tcaggagaaa tggtttcccg acgccgtata
      121 tgtggaactc gacggagtcg ggcactgtcc ccagctcgaa gtcccggccg aaaccgccga
      181 gatgatcgcc ggcaccgccc ggcttgcctg aacaccacgg acccggtccg gtcaggacct
      241 gcggccgaag aagaccgcga aaccgtggtc tttccagagg cagtcgacat cagtgaagcc
      301 gatctccctc aaccagccga gctgggtcga gagcggggtc aggtgatcgt ggctcatccg
      361 ctcgaccgtg tcggcccact cggcgtcggt cagccccttc tcgtaggcct gccagcggtg
      421 ccaacgggcg tagtgctcct ggagggccgg agtctccccc agcacatgct ctgcgttgac
      481 gaagaccccg cccggcccca actctcgaaa ggccgattca tagaccgacc gcttgccttc
      541 gtcggtcatg tgatggatgg ccagggcgga gatcacggca tcccacggtc cggccgggat
      601 cccctcgtac atgtcgccgg aaacgaccac gctcccctcc cccagcagct cgcgggcctg
      661 ttcgagcatc cggggcgaga agtcgaagag cgtgaagctc gccgagggaa acgactcgga
      721 gacgaccgca gagagcatcc cggtgccggc cccgagatcg aggacgcgaa gtggcccttc
      781 gccggatccg gccgcggcgg aagccaggcg gacagcttcg ggcaccatcc cgtagaagcg
      841 atccatctgc gggaccagct tctcgcgcgg gccccggtag gtcgcggcct gcttttcgaa
      901 tactgaggtg gcctttgcca tggtctgatc ctagaaccgc cgcgtgcgtg cgggtggaat
      961 cgggctcagc cgtcttcgac cagggggacg aaggcgaccg cgaccacccg ctcgcgggaa
     1021 agttccccgt ccttccggcg ccagagttcc agccactgcc gccaccggcc caccggcacg
     1081 atcattcgac ccccttcggc cagttgctcc ttcagggcgg ccggcacctc cccgcgagcc
     1141 gcggccgcta cgttgatcac gtcgaatggc gcctctgccg gatacccgtg cgacccgtca
     1201 ccgcagatca tttccacgtt ttcgaccccg gcatcctcca gcgactgtct ggcctgcccg
     1261 acaagttcgg ggtgccgttc gatcgagatc acccgacggg ccagccgggc cagcaccgcc
     1321 gcgtggtagc cggagccacc gccgatgtcc aggaccagat cgtcgggtcc gggcgccagc
     1381 atttcgcaca tggtcgccac caccaggggc tgggagatcg tctggccctg cccgatcggc
     1441 aacgggcgat tctcccaggc gtacggccgc tggtcctccg gcacgaagag ttccctcgga
     1501 accgaggcga tcgcatccag gacccgccgg tcggtgactt cggccgacag cgccgcgatc
     1561 agttcctctc gatcagtgtt cgcgcccatg ttccatcgat cgacttgtcc tgccccagcc
     1621 taaccccgat cctctccggc taggctggcg tcatggaaag ctcagcgcag gatcagccac
     1681 tggagccggc cagccacatg accggcgagt gcatgtgcgg caaggtcacc ttcgaggtcg
     1741 acgctcccct gatcggggcg gccctctgct actgcggccg ctgccagcac cggaccggta
     1801 ccgccttctc ggcgacggcc ctgaccaaac ccggctcttt caggatcact tcgggagagg
     1861 aacacatcgg ctcctacgag ccgggcgacg gcggcttcaa caagttcttc tgccgcgaat
     1921 gcggaggcca gctctacacc tcgcacccgc agaacccgga cattctcgcg atcaggatgg
     1981 gagccctgaa cgaggacccg gggatccgcc ccgccgccca ccagttcacc gactacgccg
     2041 ccgcctggta cccggtgccc gacgacggcc tgcccagatt cccggagcgg atcgactgga
     2101 cccagctcct ctgaaattca gcgctcgcgt ccggccagga ttgaatccag tttctcgtag
     2161 ccctcgttca ttcccttctc catgccggcc tggatcatcc cgtcgcgctg ctccctgctc
     2221 tcgaaggtcg tcttggtcac cagacgggtc tggtcgttct cgagcggaat caggttgagc
     2281 tcgtcaaccg acggcggcgg ctgaggctcc atcacgaagt tgaaggtctg gaccgcacgg
     2341 accggtctgt caagttcaac gaactcgccg aagaacctgt actcggtgcc gtcgctgtcg
     2401 atgtggacgt agctccactt cccgccgggc ctggcgtcga actcgtcgac ccgcatctcc
     2461 aggcgatccg gtcccagcca ctgcttcagc agttccggat ccatgaagca gtcccagatc
     2521 acctccggtg gcgcgtc
//
LOCUS       DAOTIP010000281         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48636_length_2535_cov_3.047581, whole genome shotgun sequence.
ACCESSION   DAOTIP010000281 DAOTIP010000000
VERSION     DAOTIP010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48636_length_2535_cov_3.047581"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="P5138_06095"
     CDS             complement(<1..1611)
                     /locus_tag="P5138_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRV60182.1"
                     /translation="MRFKGQLAVRGDAANMRARSRTALDRVGLMLAEIEATAVRPLVS
                     GLSQAEVTDLASEARASSGAGTVNMANWYRVTVPPEGAERALGILRRSPLVAVAEKAP
                     VALPPPTTPDFSNLQLHLDAAPTGTGLEPLAADSRTRGAGIRIVDLEYYWTGTHEDLQ
                     LPPSTDMGEGQYVQYTAFGDEHGTAVFGILVAKDNGFGVTGGVPDATMKGISPTESPG
                     FGYNPAGALTFLASRLARGDVVLIEQQADGPGPGSSDYVPMEWNQASFDAIRQLGDLG
                     VIVVETGANGGYDLDSPDMLGKFDRTIRDSDAILVGAGDSVTHAPLWFTSHGSRLDLQ
                     GYGNNIVTTGSVGDLQGAGPNERDIRYTNSFDGTSGAGPIVTSAVASVLSYLKAIGQP
                     PITGDQMVSLLRGTGTPQPSPGSGQIGPLPDVQSAIGLIDDSLPSVSIGGPADQSSFD
                     FDSDQSLDLDCRGGGPELESCLVVDHGPSGDASLVQGDQLPTDQPGTHTLTATAVNAL
                     GLTATSTVSYEVGPGCHSSGTRLASVESR"
     gene            1828..2529
                     /locus_tag="P5138_06100"
     CDS             1828..2529
                     /locus_tag="P5138_06100"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRV60183.1"
                     /translation="MKSSAPQATAIRHVLFEDLGLIGPILAGRGFEVTYLDAGVDELG
                     TSPADAELLVVLGGPIGVGDADRYPFLGQEIDLISRRAEAGLPTLGVCLGAQLMAAAL
                     GAGVGPTGRTEIGFSELTLTQDGRESVLAPLEGVPVFHWHGDEFEIPAGADRLASTGG
                     FPNQAFSLGSQNLALQFHIEADLRFIERWLIGHSGELATAGIDPQALRDGAARHGCQL
                     ADAAREVIESWLDGI"
BASE COUNT          407 a          825 c          848 g          455 t
ORIGIN      
        1 ccgcgattcg accgaggcca gtctggtccc ggatgaatgg catcccggac cgacctcgta
       61 ggagaccgtc gatgttgcgg tcagtccgag cgcatttacc gcggttgcgg tcagcgtgtg
      121 ggttccgggc tggtcggtcg ggagctgatc cccctggacc agggacgcat ccccggacgg
      181 accgtgatct accaccaggc aggactcgag ttcggggcca cctcccctgc agtccagatc
      241 gaggctctgg tccgaatcga aatcgaagga cgactgatct gccggaccgc cgatggacac
      301 cgaggggagg ctgtcgtcga tcagcccgat ggcggactgg acatcgggca gaggaccgat
      361 ctgccccgag ccggggctcg gctgcggggt gccggtgccg cggagcagcg aaaccatctg
      421 gtcgccggtg atcggaggtt gtccgatcgc cttcaggtaa gagaggacgg aggcgaccgc
      481 gctggtgacg atcggcccgg ctcccgaggt gccgtcgaag ctgttcgtgt aacggatatc
      541 ccgctcgttc ggtccggccc cctgcaggtc accgacgctt cccgtggtca cgatgttgtt
      601 gccgtagccc tgaaggtcaa gccgggaacc gtgtgaagtg aaccagagcg gagcgtgggt
      661 aaccgaatcc cccgctccga ccaggatcgc gtcggaatcc cggatcgtcc ggtcgaactt
      721 tccgagcatg tccgggctgt ccaggtcata tccgccgttg gcgccggtct cgaccacgat
      781 caccccgaga tcacccagtt gcctgatcgc gtcgaagctg gcctggttcc actccattgg
      841 cacatagtcc gaactgcccg gtcccggtcc gtcagcctgc tgctcgatca gcaccacatc
      901 acccctggca agtcggctgg ccaggaaggt gagcgctccc gcggggttgt agccgaagcc
      961 cggagattcg gtcggggaga ttcctttcat ggtggcgtcc ggcacaccac cggtcactcc
     1021 gaagccgttg tccttggcga ccaggattcc gaacaccgct gtcccgtgtt catcgccgaa
     1081 ggccgtgtac tggacgtact ggccctcacc catgtcggtt gacggcggca gttgcaggtc
     1141 ctcgtgagtt cctgtccagt agtactcgag gtcgacgatc ctgattccgg caccccgggt
     1201 gcggctgtcc gctgcgagcg gttcgagtcc tgtgcctgtg ggtgccgcat cgaggtgaag
     1261 ttgaaggttg ctgaagtcgg gggtggtcgg gggagggagg gccactggtg ccttttcggc
     1321 aacggccaca aggggggatc tgcgcagaat cccgagtgcc cgttcggcgc cctccggggg
     1381 cacggtcacc cggtaccagt tggccatgtt caccgtccct gccccggacg aggcccgggc
     1441 ctcgcttgcc aggtccgtga cctcggcctg gctgagtccc gacacgagcg gtcttaccgc
     1501 ggtcgcctca atctccgcga gcatcaggcc gacccggtcc agggccgttc gggatcgggc
     1561 ccgcatattc gctgcgtccc cgcggaccgc aagctgtccc ttgaagcgca actcgagaac
     1621 cctcccgccg gatggggctt cggcttgggc gagaccctgc caggccgtga agaaagccag
     1681 gagtaaacaa accagcagac cagccggccg tgccccaaca ggggccttgg tggacctccc
     1741 ccgaaccgcc ttgcctgaca tcgaattcat gctcgcacat gctgtcggcc atgccaagcg
     1801 tcccgggtca cctgagagga tgccccggtg aagtcttcgg caccacaggc aaccgccata
     1861 cggcatgtcc ttttcgagga cctggggttg atcgggccga tcctcgccgg gcgggggttc
     1921 gaggtgactt acctggacgc gggtgtggac gaactcggga cctcacccgc ggatgcggaa
     1981 ctgctggtcg tgctgggcgg cccgatcgga gtcggtgacg cggatcgcta ccccttcctc
     2041 gggcaggaaa tcgacctgat ctcccggcgt gccgaagcag ggctcccgac gctgggggtc
     2101 tgcctcgggg cccagttgat ggcggcggcg ctcggtgccg gagtcggccc gaccggcagg
     2161 accgagatcg ggttcagcga gctcacactt acgcaggacg gccgcgaatc ggttctggcg
     2221 ccgcttgaag gagtacccgt cttccactgg catggcgatg agttcgagat cccggcggga
     2281 gccgatcgcc tcgcctcgac cgggggattc cctaaccagg cgttttcact cggttcgcag
     2341 aacctcgccc ttcagttcca cattgaagcc gacctccggt tcatcgaaag gtggctgatc
     2401 ggccattccg gggagctggc caccgccggg atcgatccgc aggcgctgcg tgatggcgcc
     2461 gccagacacg gttgccagct ggctgacgct gcccgtgagg tcatcgaatc ctggctcgac
     2521 gggatctgag tccgt
//
LOCUS       DAOTIP010000282         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48903_length_2525_cov_3.297166, whole genome shotgun sequence.
ACCESSION   DAOTIP010000282 DAOTIP010000000
VERSION     DAOTIP010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48903_length_2525_cov_3.297166"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="P5138_06105"
     CDS             complement(<1..553)
                     /locus_tag="P5138_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRV60184.1"
                     /translation="MPIPENIQNFIDRQADQAPDRYDDLRAVIFNGTLKRSPEPSQTE
                     ALLQIPLGIFSRVGVKVELVRTVDHVFPPGVWPDMTEHGYEVDDFPAIYREKVVPADI
                     IILAGPIWLGDQASPTRLVIERLYAYSGDVNEAGQWSYYGKVGGAVTTGNEDGGKHVS
                     AQVLYALQHIGLTIPPQTDTYWNG"
     gene            675..>2525
                     /locus_tag="P5138_06110"
     CDS             675..>2525
                     /locus_tag="P5138_06110"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HRV60185.1"
                     /translation="MRFQSRVKRPLAAAMVVAGCLLAFDAAGAVAASPLRIEVLSSQA
                     NRVTGGDALIGVTAANSSTKRKMVLRLNGRVVTSSLSGDPANNKRKTGLVSGMKQGRN
                     WLTAWAPGQKAGSRLAVYNSPLQGPLLSGPHQEPFYCTTESLGLGPARNSNCFAPTKV
                     SWYYRASDNSFKPLANPTDRPSDMVQTTTRDGRIVDYVVRLEQGVVNRAVYNFATLAA
                     GGQIGNGWNGRFFYIYGGGCSAGHQQGQGASGGLDNDLLRRGFVVLGSSLNVFNTSCN
                     DVLSAETTSMVKEHAIEEMGRPDAWTAGMGGSGGSVQIQMIAQNYPGLLDGITPGASF
                     PDNSSPDYPDCRLLQNYFTNTTTGQNLSNAQRRAITGMTATDGGCGPLSNGADVVRAQ
                     EGCVEAVVPSSVIFDPLTNPAGVRCTLWDNMVNIYGKDPATGYARRTLDNTGIQYGLQ
                     AYLNGNINLKRFMDLNEYIGGYDDNGNLQAARSVADPEALKIAYDTGRLNTGAGNWTS
                     VPVVDRRAYQDDVSSGNVHQYVNTYRTRARLDRYNGNHDNQVMFRADGNMNVNAMDSA
                     AIDLLSTWLDAIKADDSGRSLPQKVVADKPVDAVDACWIGGNRIDGEAAIG"
BASE COUNT          488 a          842 c          765 g          430 t
ORIGIN      
        1 ccccgttcca gtaggtgtcc gtctggggag ggatggtcag cccgatgtgc tgcagcgcgt
       61 agagcacctg ggcgctcaca tgttttccgc cgtcctcgtt tccggtggtg acggcgccgc
      121 cgaccttgcc gtaataggac cactgacccg cttcattcac atcacccgaa tacgcgtaga
      181 gacgctcgat caccaggcgg gtcggcgagg cctggtcccc cagccagatc ggcccggcga
      241 gaatgatgat gtccgccggc accacctttt cgcgatagat ggcggggaaa tcatccacct
      301 cgtagccatg ttcggtcatg tccggccaga cccctggcgg gaagacatgg tcgacggtac
      361 ggacgagttc gaccttgacc ccgacccggc tgaatatccc caacggaatc tgcagcagtg
      421 cctctgtctg gctcggttcc ggcgagcgtt tgagcgtccc gttgaagatg accgcccgca
      481 ggtcgtcgta tcggtccggt gcctggtcgg cctgcctgtc gatgaagttc tggatgttct
      541 ctggaatcgg catgaagacg atcctctccg ggcgagaagg gcgcgtcaag ttgaatctca
      601 accgcaactc ggtggccgac cgggctatct actgacagac gtcagtaatc gcccaatccg
      661 cccgaggaga gtctttgcgt ttccagtcca gagtcaagcg cccgctcgcc gccgcgatgg
      721 tcgtggccgg ctgccttctc gcatttgacg cggccggagc cgttgcggcc tcaccgctca
      781 ggatcgaagt gctttccagc caggccaacc gggtgaccgg tggcgacgcg ctgatcgggg
      841 ttaccgcagc gaattcctcg acgaagcgga agatggtgct gcgcctgaac ggacgggtgg
      901 tcacctcctc cctttcgggc gatccggcca ataacaagcg caagaccggg cttgtctccg
      961 ggatgaaaca gggccggaac tggctgaccg catgggcgcc gggacagaag gccggaagcc
     1021 gcctcgccgt ctacaacagc ccgctgcagg gcccgctgct ttccggaccc catcaggaac
     1081 cgttctactg caccaccgag agcctcggac tgggcccggc ccggaattcg aactgcttcg
     1141 ctccgaccaa agtgtcctgg tactaccgtg ccagtgacaa cagtttcaag ccgctcgcca
     1201 atccgaccga ccggccttcg gacatggtcc agacgaccac ccgcgacggc cggatcgtcg
     1261 attacgtggt ccggctcgag cagggagtgg tcaaccgggc tgtctacaac ttcgccactc
     1321 tcgccgccgg aggccagatc ggaaacggct ggaacggacg cttcttctac atctacggcg
     1381 gcggctgctc ggcaggccat cagcagggcc agggcgcctc gggtggcctc gacaacgatc
     1441 ttctgagacg cggcttcgtc gtcctcggat cgagtctcaa cgtcttcaac acttcctgca
     1501 acgacgtgct ctcggccgag acgacctcga tggtcaagga acatgcgatc gaggagatgg
     1561 gcagacctga cgcctggacc gccggaatgg gcggatcggg cggatcggtc cagatccaga
     1621 tgatcgccca gaactatccg ggactgctcg acggcatcac ccccggcgcc agcttccccg
     1681 acaactcgtc accggactac cccgactgcc gtctgctgca gaactacttc accaacacga
     1741 ccacgggcca gaacctctcc aatgcccagc ggcgggcgat taccgggatg accgccaccg
     1801 acggcggctg cggacccctt tccaacggcg ccgacgtggt ccgggcccag gagggatgcg
     1861 tcgaagcggt cgtcccgtca tcggtcatct tcgatcccct caccaatccc gccggcgttc
     1921 gctgcaccct ctgggacaac atggtgaaca tctacgggaa ggatcccgcg accggctacg
     1981 cccgccggac cctggacaac acgggcatcc agtacggact ccaggcgtat ctgaacggga
     2041 acatcaacct gaaacgtttc atggatctga acgagtacat cggcggatac gacgacaacg
     2101 gcaacctcca ggccgcccgc tccgtggccg acccggaggc cttgaagatt gcctatgaca
     2161 caggtcgact caatacggga gccggaaact ggacatccgt gccggtcgtc gatcgccgcg
     2221 cctaccagga tgacgtctcg agcggaaatg tccaccagta cgtgaacacg taccggaccc
     2281 gggcccgcct ggacaggtac aacggcaacc acgacaacca ggtcatgttc cgagccgacg
     2341 ggaacatgaa cgtgaacgcg atggactcgg ccgcgatcga ccttctttcg acctggcttg
     2401 acgcgatcaa ggccgatgac tccggccggt cgctgccgca gaaggtcgtt gccgacaaac
     2461 ccgtcgacgc cgtcgatgcc tgctggatcg gtggcaaccg gatcgacggc gaagcggcaa
     2521 tcgga
//
LOCUS       DAOTIP010000283         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_48941_length_2524_cov_2.883759, whole genome shotgun sequence.
ACCESSION   DAOTIP010000283 DAOTIP010000000
VERSION     DAOTIP010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48941_length_2524_cov_2.883759"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="P5138_06115"
     CDS             complement(<1..466)
                     /locus_tag="P5138_06115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRV60186.1"
                     /translation="MSPRGVVAAGHPLSAEAGARILREGGNAVDAAVAAVLASFSLES
                     ALTGLGAGGFMIVGGPGVEPALIDFFVAAPGKGGHDLTEELIPVPVHFDETTTQVFNA
                     GAASCGVPGTAAGLALALERHGSVPMAELTAPAARLAREGAPLNEQQAYVLRI"
     gene            complement(470..1987)
                     /gene="ftsZ"
                     /locus_tag="P5138_06120"
     CDS             complement(470..1987)
                     /gene="ftsZ"
                     /locus_tag="P5138_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRV60187.1"
                     /translation="MARRKRASMREGPLADLFRSTSDEPSQEPPEFEPRTSDDTGGPQ
                     GDPIEPQAAETTPAPEPEVHSEPDPEAAGRFGRQDPLEARAEQQEPVEPAPRAEEPGP
                     LSAPDPAADTSSVPDAQDRLKRIFSEPPADRDDTPRYGRQEPSSAPGAGVPHTPVIRV
                     IGVGGGGVNAINRMIEAQIPGVEFMAVNTDLQSLQLCMADLTVHIGGEETRGLGAGAD
                     PSLGFRAAFDEQDRIKRLLKGSDMVFIAAGAGGGTGTGAAPVIARLARDIGALTVGII
                     TKPFTFEGSRRQKQAEQGIEALAGEVDTLIVIPNDRLLEVLDQSTPMVDAFQVADDVL
                     RQGVQGISDLVTLPSVINLDFADVRSIMSDAGRALLGIGMGSGPDRALIAAEKATSSP
                     LLETPMDGARSILLSVTGGPDLSLIEVSEAARAVGEAAHPDANIIFGANIDDDLDDQV
                     WITVVATRFDGKGAPPRRVERSEGSRRERSERQTRPDRGESLPAVGGGDIPEFLS"
     gene            complement(1974..>2524)
                     /locus_tag="P5138_06125"
     CDS             complement(1974..>2524)
                     /locus_tag="P5138_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRV60188.1"
                     /translation="SEVIAHREIDIAVASIDLAGAEIAMSTKIGRERSLERALKEVHS
                     DYDYICIDTPPSLGLLTINALTASSKVIVPVQCEYLSMRGLVQLQSTLKMIQENLNPN
                     VHIEGILPTMFDSRTLHAREAVSILEENFGDLVFKSRIRKAVKFAEAPVRGASVLKYD
                     PDSRAASYYRNLAKEVLSDGSS"
BASE COUNT          399 a          826 c          880 g          419 t
ORIGIN      
        1 ggatccgcag gacataggcc tgctgctcgt tcagcggcgc tccttctcgg gccagcctgg
       61 cggctggcgc cgtcagttcg gccatcggaa ccgacccgtg ccgctccagc gcgagtgcca
      121 gaccggccgc ggtgccggga accccgcatg aggcggcgcc ggcgttgaac acctgggtgg
      181 tcgtctcgtc gaagtggacc ggcaccggga tcaactcttc ggtcagatca tggccgcctt
      241 tgcccggcgc cgccacgaag aaatcgatca gggcgggttc gaccccggga ccgccgacga
      301 tcatgaaacc tccggcgccg agaccggtca gggcgctctc cagcgaaaac gaagcaagca
      361 cggctgcgac cgccgcgtcc acggcgtttc ctccctcgcg cagtatccgg gcgccggcct
      421 ccgccgaaag cggatgcccg gcggcaacga caccgcgcgg actcacgggt caggaaagga
      481 actcggggat gtcgccaccc ccgaccgcgg gcagagactc gccgcggtcc ggacgggtct
      541 ggcgctctga cctttcccgc cggctgcctt cgctgcgctc gacccgcctg ggcggggcac
      601 ccttgccgtc gaaccgggtc gccacgacag tgatccagac ctggtcgtcg agatcgtcgt
      661 cgatgttggc gccgaagatg atgttcgcgt cgggatgggc cgcctccccc acggcacggg
      721 ccgcctcgga gacctcgatc agcgagagat ccgggccgcc ggtgaccgag agcaggatcg
      781 aacgtgcccc gtccatcggg gtttcgagca gcggcgagga agtcgccttc tcggcagcga
      841 tcagggcccg gtcggggccg gaacccatgc cgatgccgag cagcgcccgg ccggcgtcgg
      901 acatgatcga tcgaacgtcg gcgaagtcga gattgatcac cgagggcagg gtgacgagat
      961 cggagatgcc ctggacgccc tggcgcagca cgtcgtcggc gacctggaag gcgtcgacca
     1021 tcggggtcga ctggtcgagc acctccagca gacggtcgtt cgggatcacg atcagggtgt
     1081 cgacttcgcc ggccagggcc tcgatgccct gttctgcctg cttctggcga cggctgccct
     1141 cgaaggtgaa cggcttggtg atgatgccga ccgtgagcgc accgatgtcg cgggcaaggc
     1201 gggcgatgac cggcgctgcg ccggtacctg tgccaccgcc ggcgccggcg gcgatgaaga
     1261 ccatgtccga gcccttgagc agtcgcttga tccggtcctg ctcgtcgaag gccgcacgga
     1321 acccgagcga gggatcggcg ccagccccga ggccacgggt ttcttcgccg ccgatgtgga
     1381 cggtgaggtc ggccatgcag agctggagcg actggaggtc ggtgttcacc gccatgaact
     1441 cgacgcccgg gatctgggcc tcgatcatgc ggttgatcgc gttcacgccg ccaccaccga
     1501 caccgatgac gcggatgacc ggggtatgcg gcactcccgc acccggagct gaagacggct
     1561 cctggcggcc gtagcggggc gtgtcgtccc ggtcggccgg gggctcggag aagatcctct
     1621 tcagacgatc ctgggcatcc ggcaccgaac tcgtgtcagc ggccgggtcc ggcgccgaaa
     1681 gcgggccggg ctcttcggcc ctgggggccg gttccaccgg ttcctgctgt tcggctctgg
     1741 cttcgagcgg atcctgtcgg ccgaaacggc cggcagcttc gggatccggc tcggagtgga
     1801 cctccggctc gggcgccggg gtggtttccg ctgcctgcgg ctcgatcggg tcgccctgcg
     1861 gtcctccggt gtcgtcggag gtccggggtt cgaactcggg gggttcctgc gaaggttcgt
     1921 cggaggtaga gcggaacagg tccgccagcg gaccttcacg catactcgct cgcttacgac
     1981 gagccatcac tcaagacctc cttcgccaaa ttccggtagt acgacgccgc acgactgtcg
     2041 ggatcgtact tcaggacact agcgcctcgt accggcgcct cggcgaactt cacggccttg
     2101 cggatgcggg acttgaagac gagatccccg aaattctctt cgaggatgga gacggcctcc
     2161 cgcgcgtgaa gggtgcggga gtcaaacatg gtcggcagga tgccctcgat gtggacgttc
     2221 ggattgaggt tctcctgaat catcttcagg gtgctctgga gctggaccag accacgcatc
     2281 gagaggtatt cgcactggac cggaacgatc accttgctcg aagcggtgag cgcgttgatc
     2341 gtcagcaggc ccaggctcgg cggggtgtca atgcagatgt agtcgtaatc ggaatggact
     2401 tccttcagcg cgcgctccag tgagcgttcg cggccgatct tcgtgctcat cgcgatctcg
     2461 gcgccggcca gatcgatcga ggcgacggcg atgtcgattt cccggtgggc gatcacttcg
     2521 gaga
//
LOCUS       DAOTIP010000284         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_49021_length_2521_cov_3.038118, whole genome shotgun sequence.
ACCESSION   DAOTIP010000284 DAOTIP010000000
VERSION     DAOTIP010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49021_length_2521_cov_3.038118"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="P5138_06130"
     CDS             <1..628
                     /locus_tag="P5138_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HRV60189.1"
                     /translation="GEVVREALVAINEFAGAVEIDPTPQEVSWTVPLDQDDEHRIYDP
                     AQVKEYFEMATAAGMALARFRAPYRGRSTPLNAWWGSFDLAVSLFSGRPADPPSQDFI
                     MRNAMDSQEVAVGWWPGDDRYPKPAFYAYAHPAEDGYSGRDLSPVPGGWNDELGEYVL
                     DHEAAAVTGNPEQAVIDFCGEIFSHACNVCDWNPALAASAAGDPPPIR"
     gene            complement(645..2348)
                     /gene="polX"
                     /locus_tag="P5138_06135"
     CDS             complement(645..2348)
                     /gene="polX"
                     /locus_tag="P5138_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRV60190.1"
                     /translation="MTNAQIAEALEELAVLYQLDGADQYRVLAYTNSARTIKNEGRSV
                     EEMARNGTITELPGVGKTLEEKILALVETGEIPAAAKLKEKIPVGLLEISRIPGLGPK
                     TVRRLHDELDVTGPEDLRAAAEAQKVRELKGLGPKVEEKILAGLDKLEAEPEGPTRLR
                     LDEILPTAQDLVEALRSEPSCDKAEIAGSVRRLTETCHDIDLIATSTDPKALATEIAG
                     HELVGEHGKPSDKGVKLTTNSGIDVDVRIVEPDAFGNLLQHFSGSGAHNAELRERAVA
                     KGLHVSENGIKHEKTGEVELFTTEEEVYERLGYQYIPPELREDRGELDAAEKGELPDL
                     VERSQIKGDLHCHTTLSDGVASMEEMAAAAEALSYEYLAITDHSLSHGFGNNVEPDRL
                     WQRIEEIAEFNNEDHAIRLLSGSEVNIHPNGSVDYDDDLLEALDWVIASVHTAFDEDE
                     DKMTGRMITAIENPLVDCIGHPTGRLINQRDPYPLDMERVIAAAAENDTLIEINGNPR
                     RRDLNEIHARMAAEAGVGIVINTDAHRPDTLDFMDYGIATARRAWLTAEQVKNTGSWW
                     K"
BASE COUNT          370 a          833 c          829 g          489 t
ORIGIN      
        1 cggcgaggtg gtgagggagg ccctggtcgc gatcaatgag ttcgccggtg cggtcgagat
       61 cgatccgacg ccccaggaag tgagctggac ggttccgctc gaccaggacg acgagcaccg
      121 gatttacgac ccggcccagg tcaaggaata cttcgagatg gcgaccgccg ccggaatggc
      181 cctggcccgc ttccgggctc cttaccgggg ccgttcgact ccgctcaacg cgtggtgggg
      241 ttccttcgac ctggcggtca gcctgttctc cgggcgcccc gcggatccgc cgtcgcagga
      301 cttcatcatg cgcaacgcaa tggactccca ggaagtggcg gtgggctggt ggccaggcga
      361 cgaccgctat ccgaagcccg ccttctacgc ctacgcccat cccgccgagg acggttactc
      421 aggccgggat ctctcaccgg tcccgggcgg ctggaacgac gaactcggcg aatacgtgct
      481 cgaccacgaa gcggccgcgg taaccggcaa ccccgagcag gcggtgatcg acttctgcgg
      541 cgagatcttc agccacgcct gcaatgtctg cgactggaac ccggccctgg ccgcaagcgc
      601 cgctggcgac cctcccccga tccgctgagc cgggccggac cgggctactt ccaccaggag
      661 ccggtgttct tcacctgctc cgcggtcagc caggcgcggc gggcggtggc tatcccgtag
      721 tccatgaaat cgagggtgtc cggccggtgg gcgtcggtgt tgatcacgat gccgacccct
      781 gcctcggcgg ccatccgggc gtgaatctcg ttcaggtccc ggcggcgggg gttgccgttg
      841 atctcgatca gggtgtcgtt ctccgcggcg gcggcgatca ccctttccat gtcgaggggg
      901 taggggtccc gctggttgat cagccggccg gtcggatggc cgatgcagtc gaccagcggg
      961 ttctcgatcg cggtgatcat ccggccggtc atcttgtcct cgtcctcgtc gaaggcggtg
     1021 tggaccgagg cgatcaccca gtcgagggct tccagcaggt cgtcgtcgta atcgaccgac
     1081 ccgttcggat gtatgttcac ctcggaaccg gagagcaggc ggatggcgtg atcctcgttg
     1141 ttgaactcgg cgatctcctc gatccgctgc cagagccggt ccggctcgac gttgttgccg
     1201 aagccgtgtg acagggagtg atcggtaatc gccagatact cgtagctgag agcctcggcc
     1261 gcggcagcca tctcctccat cgacgcgacg ccgtcggaaa gggtggtgtg acagtggaga
     1321 tcacccttga tctggctgcg ctctaccagg tcaggaagtt cgcctttttc ggcggcgtcc
     1381 agttcacccc ggtcctccct gagctccggc gggatgtact gatagccgag ccgctcgtag
     1441 acctcctcct ccgtggtgaa tagctcgacc tcgcctgtct tctcgtgctt gatcccgttc
     1501 tcggacacgt gaagtccttt cgcgacagcc cgttcgcgca gctccgcgtt gtgggcgccg
     1561 gagccggaga aatgctgaag caggttgccg aaggcatcgg gttccacgat ccgcacgtcg
     1621 acgtcgatgc ccgaattggt ggtcagcttc actcccttgt cgctgggctt gccgtgttca
     1681 ccgaccagtt cgtgcccggc gatctcggtc gccagggcct tcgggtcggt cgaggtggcg
     1741 atcaggtcga tgtcatggca ggtctcggtc agcctgcgga ccgagccggc gatctcggcc
     1801 ttgtcgcaac tgggctccga gcggagcgcc tcgaccaggt cctgtgcggt gggcaggatc
     1861 tcgtccagcc tgagcctggt tggcccttcc ggttccgcct ccagtttgtc caggccggcg
     1921 aggattttct cctcgacctt cgggccgagg cccttcagtt cacggacctt ctgcgcctcg
     1981 gcggcggctc gcaggtcttc cggtccggtc acgtccagct catcgtggag acggcgcacc
     2041 gtcttcggtc cgagtccggg gatgcggctg atctcgagca ggccgaccgg gatcttctcc
     2101 ttcagctttg cggccgccgg gatctcgccg gtctcgacca gggcgaggat cttctcctcg
     2161 agggtcttgc ccactcccgg caactcggtg atcgtcccgt tccgcgccat ctcctcgact
     2221 gaccggccct cgttcttgat cgtccgggcg gagttcgtgt aggccagaac cctgtactgg
     2281 tcggccccgt cgagctggta gagaacggcc agctcctcga gcgcttcggc gatctgggcg
     2341 ttggtcatga ccagaaccta cccaccggcg tccgggggat acgatccgcc ggtgccggat
     2401 gaagtcagga cagagcatcg aggcgcggcc tgggtaattc tgccgaccta caacgaagcc
     2461 ggaaacatcg gcccgatggt cgatgccctc ggcccgaagc tcactcacga tgacgaccgg
     2521 a
//
LOCUS       DAOTIP010000285         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_49346_length_2509_cov_2.957213, whole genome shotgun sequence.
ACCESSION   DAOTIP010000285 DAOTIP010000000
VERSION     DAOTIP010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49346_length_2509_cov_2.957213"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="P5138_06140"
     CDS             complement(<1..559)
                     /locus_tag="P5138_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRV60191.1"
                     /translation="MTSIETGTEKVRLDFPAESVARLTISNPPKRNALDHEILDAITA
                     TVPRLDSGIEIRCLLITGEDPVFSAGYDIGDIPAESFEQDAEALVAHPFTAALDAIEA
                     FPWPTVAALNGHCLGGALEMAVTCDLRVSAPKAKVGMPPAKLGLIYGHTGLIRFIRTI
                     GLPRTRELFFTGDMLTAARADEIGLV"
     gene            complement(620..832)
                     /locus_tag="P5138_06145"
     CDS             complement(620..832)
                     /locus_tag="P5138_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRV60192.1"
                     /translation="MPNIEAHITGNVWKIQVKVGDEVSEDDEVIILESMKMEIPVESE
                     DDGVVKEILCEEGQSVTEGDVLIVLE"
     gene            900..1658
                     /locus_tag="P5138_06150"
     CDS             900..1658
                     /locus_tag="P5138_06150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV60193.1"
                     /translation="MESSNANSLAVLIAARNEADVIADTIRSLREAFPGAALWVADDA
                     SDDGTSEAAMAAGAKVVRRGKSHGKGGNVTACAEAMLSDPDLPELVLLCDGDLAGSAG
                     DLAPLVDAVRIGDCDLAVAMFARRVGGGFGFALGFSAWAIRNRCGAEPQAPISGQRAM
                     RLEVLRAVLPFAPSYGMETGMTIDAVRAGYRLGEYEIDLAHKATGKSFRGFVHRFRQL
                     IDFARVWWSRRGGRGPDGKKPARSPDRRKQETIS"
     gene            1655..>2509
                     /locus_tag="P5138_06155"
     CDS             1655..>2509
                     /locus_tag="P5138_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRV60194.1"
                     /translation="MILAIDQGTTGSTALVFDEMGKIRGRGYAEFKQHFPQPGWVEHD
                     AEEIWETTWGVAARALAGAGISGPDLDAIGITNQRETVVAWDPETGKPLHRALVWQDR
                     RGAARCNELKAEGLEPLVRERTGLVLDPYFSATKIEWLRQNVEGADKAVFGTIDSWLV
                     QRMCGEHVTDLTNASRTMLFDTGKLEWDPELCAAFGVEPESLPEVRPSAGIFGTTSGF
                     GGEVPVAGIGGDQQSALFGQMCHEPGMAKNTYGTGNFVLLNAGREKPDPGEGLLGTIA
                     WGIGDAGSA"
BASE COUNT          419 a          787 c          891 g          412 t
ORIGIN      
        1 tgaccaggcc gatctcgtcg gcccgggcgg cggtcagcat gtcgccagtg aagaagagct
       61 cgcgggtgcg gggcaggccg atcgttcgga tgaagcggat caggccggtg tgtccgtaga
      121 tcaggccgag cttggccggg ggcatgccca ccttcgcctt cggcgccgaa acgcggagat
      181 cacaggtgac ggccatctcc agcgcgccgc cgaggcaatg gccgttcagg gcagccaccg
      241 ttggccaggg aaaggcttcg attgcgtcga gggccgcggt aaacggatgg gcgaccaggg
      301 cctcggcgtc ctgctcgaag ctctcggccg gaatgtcacc gatgtcgtag ccggccgaga
      361 agaccgggtc ctcgccggta atcagcaggc agcggatctc gatgccggaa tcaagtcgcg
      421 gaacggtcgc cgtgatcgca tcgaggatct cgtgatcgag ggcgttccgc ttcggcggat
      481 tcgaaatcgt gaggcgggcg accgattcgg ccgggaagtc gagccggacc ttctccgtgc
      541 cggtttcgat tgaagtcacg tcggccattt ctctcgctcc ggctttgctt cgcggttgcc
      601 gggagactcc gggtcctgac tactccagga cgatcaggac gtcgccttcg gtcacggact
      661 ggccctcttc gcagaggatc tccttgacca cgccgtcgtc ctcggactcg accgggattt
      721 ccatcttcat cgactcgagg atgatcacct cgtcgtcctc ggagacctcg tccccgacct
      781 tgacctggat cttccagacg ttcccggtga tgtgagcttc gatgttcggc atgggctgga
      841 ctctagtcga aggacaaagg gtccggctgc cgcgccgggc gcagataaag tccgctgcgg
      901 tggagagttc gaacgccaat tccctcgcgg tacttatcgc cgcgcgaaac gaggccgacg
      961 tgatcgccga cacgattcgg tcgttgcggg aagcctttcc cggggcagcc ctgtgggtgg
     1021 cggacgacgc ctccgatgac ggcacttccg aggcggcgat ggcggccggg gcgaaggtgg
     1081 tgcggcgggg aaagtcccac ggcaagggag gcaatgtgac cgcctgtgcc gaggcgatgc
     1141 tgagcgaccc ggacctgccc gaactggtgc tgctctgcga tggagacctg gccggatcgg
     1201 ccggcgatct cgccccgctg gtcgatgcgg tgagaatcgg tgactgcgac ctcgcggtcg
     1261 cgatgttcgc caggcgtgtc gggggcggat tcggtttcgc gctcggtttc tccgcctggg
     1321 cgatcaggaa ccgctgcggg gccgaaccgc aggcgccaat ctcgggacag cgggcgatga
     1381 ggctggaagt gcttcgcgcg gtgctgccct tcgcaccctc ctacggaatg gagaccggga
     1441 tgacgatcga cgcggttcgc gcgggctacc ggctgggcga gtacgagata gacctcgccc
     1501 acaaggcgac cggcaagagc ttccggggat tcgtccaccg tttccgccag ctgatcgact
     1561 ttgcccgcgt ctggtggtcg cggcggggcg gccgcggtcc ggacggaaag aagccggccc
     1621 gcagcccgga ccggcgaaag caggagacga tctcgtgatc ctggcgatcg atcagggcac
     1681 caccgggtct accgcgctcg tctttgacga gatggggaag atccggggcc gcggttacgc
     1741 cgagttcaag cagcatttcc cgcagcccgg ctgggtcgag cacgacgccg aagagatctg
     1801 ggagacaacc tggggggttg ccgcccgggc gcttgcgggg gccggcatca gcggtcccga
     1861 cctcgacgcg atcggcatca ccaaccagcg cgagacggtc gtcgcctggg acccggaaac
     1921 cgggaagccg ctccaccggg ccctggtctg gcaggaccgt cgcggagcag cccgatgcaa
     1981 cgagctcaag gccgagggcc tcgaaccgct ggtccgtgaa cgaaccggac tggtgctcga
     2041 cccgtacttc tcggcgacca agatcgaatg gctcaggcag aacgtggaag gagccgacaa
     2101 ggccgtcttc gggacgatcg actcctggct ggtgcagcgg atgtgcgggg agcacgtgac
     2161 cgacctgacc aacgcctctc ggaccatgct gttcgacacc ggaaagctcg aatgggaccc
     2221 ggagctttgc gcggccttcg gggtggaacc ggagtcgctc ccggaagttc gaccttccgc
     2281 ggggatcttc ggcaccacct ccggtttcgg cggcgaggtt ccggtggcgg gcatcggcgg
     2341 cgaccagcag tcggccctct tcggtcagat gtgccacgag ccggggatgg cgaagaacac
     2401 ctacggcacc ggcaacttcg tcttgctgaa cgccggccgg gagaagccgg acccggggga
     2461 agggctgctc ggcaccatcg cctggggaat cggagacgcc ggatccgcc
//
LOCUS       DAOTIP010000286         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_49517_length_2501_cov_5.360589, whole genome shotgun sequence.
ACCESSION   DAOTIP010000286 DAOTIP010000000
VERSION     DAOTIP010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49517_length_2501_cov_5.360589"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="P5138_06160"
     CDS             <1..1027
                     /locus_tag="P5138_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV60195.1"
                     /translation="TVALSSVIDAGATLALGRTFSASKFWAEVIAADSTAFVYIGELC
                     RYLLNQPPKPTDRAHRVRVIVGNGLRPEIWERFTTRFGIKRVVEFYAASEGNVAFINV
                     FNIPGSTGISPMPLAYVDYDLETGEPARGPDGRVRRVRPGRPGLLLSPVTRLSPFDGY
                     TDKAASEKKLVRNAFKDGDVWFNTGDVMSPQGMGHAAFTDRLGDTFRWKGENVATTDV
                     EAALAADPTVEECTVFGVEVPDTGGRAGMAAVALRAGAQFDGRALARHLYGHLPGYAV
                     PLFIRIVESLEATSTFKSRKVDLRSQGYGPEVSDPLYVLNGREVGYVPFYDAYPVDVA
                     AGKRPRA"
     gene            1338..2276
                     /locus_tag="P5138_06165"
     CDS             1338..2276
                     /locus_tag="P5138_06165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60196.1"
                     /translation="MLDEYTAKCLRVFAGLGREFSEPEQAHLRSVLERQLGEAFSRSQ
                     RSVVTVSYRSPVSASLSYEVTISAPTIEEVYREWVANRKPPLFGVEPDARVWALSAEA
                     PDPAQCPVLEIGAGTGRNALALAKRGHPVDVVEITTAFADIIGQAAQRDSLPVRVINR
                     DVFQADGDLRRDYGLIVLSEVVSDFRSPGQLRGLLELASRCLSPAGRLVFNIFLTRGD
                     YVPDDAARQFGQQVYTSFFTRDELTEACDGLPLRLESDDCVHDYEKQNLPDGAWPPTG
                     WYAAWVSGRDAFDVDFEQRPIDMRWLVYRRTVTASR"
BASE COUNT          383 a          798 c          883 g          437 t
ORIGIN      
        1 gaccgtcgcc ctgtcctcgg tgatcgacgc gggcgcgaca ttggctttgg gcaggacctt
       61 ttcggcttcc aagttctggg ccgaggtcat cgcggcggat tcgacggcgt tcgtctacat
      121 cggggagctg tgtcggtatt tgctcaacca gcccccgaaa ccgaccgacc gcgcccaccg
      181 ggtccgggtg atcgtcggca acggactgcg gcccgagatc tgggaacggt tcacgacccg
      241 gttcgggatc aaacgggtgg ttgagttcta cgccgccagc gagggcaacg tcgcgttcat
      301 caacgtcttc aacattcccg ggtcgaccgg gatcagtccc atgccgctgg cctacgtcga
      361 ctacgacctg gagaccgggg agccggcgcg cgggccggac ggccgggtgc gtcgagtccg
      421 cccgggccgg ccggggctgc tgctgagccc ggtgactcgg ctctcgccct tcgacgggta
      481 caccgacaaa gcggccagcg agaagaagct ggtgcgtaac gccttcaagg acggcgacgt
      541 ctggttcaac accggcgatg tcatgagccc gcagggtatg ggccacgcgg ccttcaccga
      601 tcggctcggt gacactttcc gctggaaggg cgagaacgtt gccaccaccg atgtggaggc
      661 ggccctggcc gcggacccga ccgtggagga atgcacggtg ttcggcgtgg aggttcccga
      721 taccggcggc cgggccggga tggcggcggt cgctctgcgt gccggtgcgc agttcgacgg
      781 ccgggcattg gcccgccatc tttacggcca cctgcccggt tatgcggtgc cgttgttcat
      841 ccggatcgtg gaatcgctgg aggccacctc gacgttcaag agccgcaagg tcgatctgcg
      901 cagtcagggt tacggtcctg aggtgtccga cccgctgtat gtcctgaacg ggcgcgaggt
      961 gggttatgtg ccgttctacg acgcctaccc ggtcgacgtg gcggccggga agcgtccgag
     1021 ggcctgaggg acgaaggccc gaggagcgct gacctgttgg gtgctgaagc gtccgagggc
     1081 ctgagggacg aaggcccgag gagcgctgac ctgttgggtg ctgaagcgtc cgagggcctg
     1141 agggacgaag gcccgaggag cgctgaatcc cgttctgcta ctttcgcgtc cagccgagta
     1201 ccgacggggg tggatcgaga atggatgtgg agtccccaca gtcaatcggt gcggaccagc
     1261 tcgcgcaggg gatggcacgc cggcgcgact cccgcatcgc agccgagggg gcggtttcct
     1321 tcccggccgt gccggccatg ctcgacgagt acaccgccaa gtgccttcgc gtgttcgccg
     1381 gcctgggtcg ggaattcagc gaacccgaac aggcccacct caggtcagtt cttgagcgcc
     1441 agctcggcga ggctttttcg cgttcccaac gctccgtcgt caccgtgagc taccggtctc
     1501 cggtgtcggc ctcgctgagc tacgaggtca ccatctcggc cccgacgatc gaggaggttt
     1561 accgggagtg ggtggccaac cgcaagccgc cgctgttcgg ggtcgagccg gacgcccggg
     1621 tgtgggcgct gtccgcggag gcgccggatc cggcgcaatg tccggttctg gagatcgggg
     1681 cgggtaccgg ccgcaatgca ctcgcgctgg caaagcgcgg gcaccccgtc gacgtcgtgg
     1741 agatcaccac cgccttcgcc gacatcatcg ggcaagcggc ccaacgcgat tcgttgccgg
     1801 tcagggtgat caaccgcgac gtcttccagg ccgacggtga tctcagacgc gactacgggc
     1861 tgatcgtcct gtccgaggtg gtgtcggatt tccgatcccc ggggcagttg cgcggtctgc
     1921 tcgaactggc gtcgcgctgc ctgtccccgg cggggcggct ggtcttcaac atctttctca
     1981 cgcgtggcga ctacgttccc gacgatgcgg cacgccagtt cggtcagcag gtctacacct
     2041 cgttcttcac ccgcgatgag ctgaccgagg cctgcgacgg cctgcctttg cggctggaat
     2101 ccgacgactg cgtccacgac tacgagaaac agaacctgcc ggacggagcc tggccgccga
     2161 ccggctggta cgcggcgtgg gtgagtggcc gggacgcctt cgacgtcgat ttcgagcagc
     2221 gtccgatcga catgcgctgg ctggtctatc gaaggacagt gacagcgtca cggtgagccg
     2281 cccgggcccg gtggatgtgc gcggcggccc acccggcagg caccatgtgg ggatgaccgg
     2341 gacattctgt ggcaggtccg tggcggctga ccgggcgctg atcatggcga tcgtcaatcg
     2401 cacacccgat tccttctatg accggggtgc gaccttctcc gacgacgcgg cgatggcagc
     2461 cgctcaccgt gtcatcgccg agggtgccga tgtcgtcgac g
//
LOCUS       DAOTIP010000287         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50031_length_2482_cov_3.213432, whole genome shotgun sequence.
ACCESSION   DAOTIP010000287 DAOTIP010000000
VERSION     DAOTIP010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50031_length_2482_cov_3.213432"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="P5138_06170"
     CDS             <1..1385
                     /locus_tag="P5138_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRV60197.1"
                     /translation="FHYVPFPGTGIQGIQQVEPGTMVTRSADGSTEVRRWTPLFFDRA
                     PDGQDVGLAELENALLESVSRQLVADVEVGIFLSGGVDSSLLAALAVESGARPRAFTV
                     SFQGHGDYDESDRAARLAQSLGIPHQIETMDTGFLEAVDGIAGAYDVPFADSSAIPML
                     ALSRLARSEVTVAMSGTGGDDLFAGYYRHRAHKLRSAVGFLPDQLLRRLSEIKVDQGG
                     ERRSGLNLARSYVSRIARAGAGDDHEQYLALVAGSTSDAGHEAFRGIGESRAASEGVA
                     SRLGLDNPSSGSILREIQRFEMRSYLPGDLLTKEDRASMSVGLEARVPLLDEAVAALA
                     DRMPDRQKLSLRGGKLPLRQIAAQKVPAVGGGGRKRGFAVPLHDLFAGRWRSEAGTWF
                     REGSTSLIDGTAAADLLNSGTSHATDLWALAALTAWERRLDKCRSAALELRKGLAGDP
                     TDEPTGGRFT"
     gene            1385..2398
                     /locus_tag="P5138_06175"
     CDS             1385..2398
                     /locus_tag="P5138_06175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV60198.1"
                     /translation="MLSSGSTVLVTGGGGFIGSHLAERLRNDGHKVRVLDNFATGSRE
                     NVRHLLDDIELIEGDMQSYERAHNAVRGCDVVFHQAALPSVPRSIQDPLTSNASNVIG
                     TLNLLLAARDEGVDRFVFASSSSVYGANPQLPKVETMATLPISPYAVAKQAAEGYCRA
                     FYEVYGLETVALRYFNVFGPRQDPLSQYAAVIPKFITSFLEGRQPVVFGDGEQSRDFT
                     YIDNTVDANILAANAEGVAGRAFNIACGERISLNDLLSKLRELTGSSLSAQYLDPRPG
                     DIPHSLADVTEARQSLGFSPQVDLLDGLARTVEYHREISPGPGDLAGTDSSEEIGISN
                     SGR"
BASE COUNT          427 a          752 c          818 g          485 t
ORIGIN      
        1 ccttccatta cgtgccgttc cccgggaccg ggattcaggg catccaacag gtcgagccgg
       61 gaacgatggt cacgcgcagc gctgacggaa gcaccgaggt caggcgctgg accccgctgt
      121 ttttcgatcg tgcacccgac ggtcaggatg tgggtctcgc tgaacttgaa aatgccttgc
      181 tggagtcggt ttcgcgtcag ctggtcgcgg acgtggaagt cggcattttc ctctctggcg
      241 gagtggattc ctccttgctg gccgcgttgg ccgtggaatc gggtgcccgc ccgagggctt
      301 tcacagtctc tttccagggg cacggggact acgacgagtc cgatcgggcc gcccggctcg
      361 cgcagtcgct gggaattccc catcagatcg aaacgatgga caccggattc ctggaagcgg
      421 tcgacggcat tgccggtgcg tatgacgttc ctttcgctga ctcgtccgca attccgatgc
      481 tggcgctctc gcgtctcgcc cggtccgaag tcacggtggc gatgagcggc accggcgggg
      541 acgatctctt tgccggctac tacaggcacc gtgcccacaa actgcggtcc gccgtcggtt
      601 tcctgccgga tcagctgctc aggcgcctga gtgagatcaa ggtcgatcag ggcggagagc
      661 ggcgtagcgg gttgaatctg gcccgctcct acgtttcgcg catcgccagg gcgggggcag
      721 gcgacgacca cgagcaatac ctggcgctgg tcgccgggtc gacctcggac gcggggcacg
      781 aagcttttcg cgggatcggg gagagcaggg cagcttcgga gggcgtcgcg tcccgtctcg
      841 ggctggacaa cccttcctcg ggatcgatac ttcgcgagat acagcgattt gagatgaggt
      901 cgtatctgcc cggtgacctg ctcacgaagg aggatcgggc aagcatgtcg gtcggactcg
      961 aggcaagggt cccgttgctg gacgaggcgg ttgcggcttt ggctgaccgg atgccggacc
     1021 gccagaagct gtcacttcgc ggcggcaagc ttccattgcg acagatcgcc gcacagaaag
     1081 tcccggcggt tggcggcggc gggcggaagc gtggattcgc cgttccgctt cacgatctgt
     1141 tcgccggccg ttggcgaagc gaggccggga cctggttcag ggagggctcg acatctttga
     1201 tcgacggaac cgccgcggca gacctgctca actccggtac ttcccatgcc acggatctct
     1261 gggcgctggc ggcgctgacc gcctgggagc gacggctcga caaatgtcgc agtgctgccc
     1321 tggagttgcg gaagggactg gcaggtgacc cgactgacga acccaccggt ggtaggttca
     1381 cgtgatgctt tcaagcggca gtactgtgct cgtcacgggt ggcggaggtt tcatcggatc
     1441 ccacctggcc gagcgacttc gaaacgacgg acacaaggtc cgggtacttg acaatttcgc
     1501 gaccggcagt cgcgaaaacg tccggcacct tctcgatgac atcgagttga tcgaaggcga
     1561 catgcagagc tacgaacggg cccacaacgc ggtacggggc tgcgacgtgg tctttcacca
     1621 ggcagcgctg ccgtcggtgc cgcgatcgat ccaggatcct ttgaccagca acgcctccaa
     1681 cgtcatcggc acgctgaatc ttcttctggc ggcccgggac gagggcgttg acaggtttgt
     1741 tttcgcgtcg tcttcttctg tttacggcgc caatccgcaa ctccccaagg tggagacgat
     1801 ggcgactttg ccgatttcgc cctatgcggt tgcgaagcag gctgcggagg gatattgccg
     1861 ggccttctat gaggtctacg gtctcgaaac cgtggcgctc aggtacttca acgtgttcgg
     1921 acccaggcag gatccgcttt cgcagtacgc cgcggtgatt ccgaagttca tcacttcgtt
     1981 cctggaaggg cgtcagccgg tcgttttcgg agatggagaa cagtctcgcg acttcaccta
     2041 catcgataac acggttgacg cgaacatcct cgcggccaac gcggaagggg tcgccgggcg
     2101 ggccttcaac atcgcctgcg gcgagagaat cagcctgaac gacctgctgt ccaagttgag
     2161 ggaactgacc ggcagcagtc tttcggctca gtacctcgac cctcgtcccg gggacatccc
     2221 ccattctctg gccgatgtga cggaggcccg ccagtcgctc ggtttcagtc cgcaggtcga
     2281 tcttctcgac ggtctggctc gcaccgtcga gtatcaccgg gagatcagtc ccggaccggg
     2341 tgacctggcc gggacggatt caagcgaaga gatcggcatc tcgaactcag ggcggtaggt
     2401 cggtcactcg aattgctgcc ggtcctcgcc gaaaccgcct atccagactc agccccggga
     2461 gctcgggtca ggcttggcga ct
//
LOCUS       DAOTIP010000288         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50516_length_2464_cov_3.527190, whole genome shotgun sequence.
ACCESSION   DAOTIP010000288 DAOTIP010000000
VERSION     DAOTIP010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50516_length_2464_cov_3.527190"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="P5138_06180"
     CDS             complement(<1..187)
                     /locus_tag="P5138_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRV60199.1"
                     /translation="MSDLKRIIPCLDVDNGRVVKGTNFVDIRDAGDPVELAERYDAEG
                     ADELVFLDITASHEGRDT"
     gene            complement(184..909)
                     /gene="hisA"
                     /locus_tag="P5138_06185"
     CDS             complement(184..909)
                     /gene="hisA"
                     /locus_tag="P5138_06185"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRV60200.1"
                     /translation="MILYPAIDILDGKAVRLLKGEYDQETRYFDDPADAARQWADGGA
                     EYIHVVDLDGAKTGEPVNLGRIESIAAAVDCPIEVGGGIRDLETAERILDAGAARVVV
                     GTAAMRDREFLDALIAERGAEKVVVGIDARGGQVSLAGWTESSGVDVADAAAELTRRG
                     VVRFLFTPIEVDGTMEGPNIPELIRVAGATDASVIASGGVGNLGHLEDLARRAPSNVE
                     GVIVGKALFEGKFTVEEGIEALR"
     gene            complement(906..1526)
                     /gene="hisH"
                     /locus_tag="P5138_06190"
     CDS             complement(906..1526)
                     /gene="hisH"
                     /locus_tag="P5138_06190"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRV60201.1"
                     /translation="MGADPKIAILDYGMGNLRSVEKALEKVGARAIVTADEVEIDSAD
                     GLVLPGVGAFPKAMEKIRELELDRMLRDAVAEDKPVLGICLGLQLLFESSVEQGGAEG
                     LGLLEGDVVQVPAGGRKIPHIGWAEVTWAKPDPLTEGLRPGEPFYFVHSFVVQPTDSE
                     LIGTASYGGRFACMAGRGKVWGAQFHPEKSSAAGLRMLRNFREICS"
     gene            complement(1548..2138)
                     /gene="hisB"
                     /locus_tag="P5138_06195"
     CDS             complement(1548..2138)
                     /gene="hisB"
                     /locus_tag="P5138_06195"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRV60202.1"
                     /translation="MSRQARIERKTGETSIELALDLDGGEYSAETGVGFFDHMLDLLA
                     RHANIGLDVKATGDLETGSHHTVEDVGIVIGQAIDQALGDRAGIRRYGSAVVPMDEAC
                     AECALDFSGRALSVFRGDIPVTSIANYETELTEEFFRAVAGGAKMTLHIDIRYGSNVH
                     HMIEAAFKAFARALRVAVSIDPDEKGVPSTKGTLNG"
     gene            complement(2135..>2464)
                     /locus_tag="P5138_06200"
     CDS             complement(2135..>2464)
                     /locus_tag="P5138_06200"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRV60203.1"
                     /translation="HLELMSEDSAALAGAVRTAGCVFTGQEAGTAFGDYVAGSNHVLP
                     TDGTGRIFGPLSPATFRRGTARVSLDADSARRLAGPLEALARAEGLPVHGLSATARAQ
                     KHDQEKS"
BASE COUNT          395 a          865 c          760 g          444 t
ORIGIN      
        1 tcgtgtccct gccctcgtgc gaggccgtga tgtcgagaaa gaccagttcg tcagcacctt
       61 cggcgtcata cctttcggcc agttctaccg ggtcgccggc gtcccggatg tcgacgaagt
      121 tggtgccctt cacgacgcgc ccgttgtcga catcgagaca ggggatgatc cgtttgagat
      181 cgctcatcgc agggcctcaa tcccttcttc gacggtgaac ttcccttcga acagggcctt
      241 gccgacgatc accccctcca cgttggatgg cgccctccgg gccaggtctt caaggtgtcc
      301 caggttcccg accccgccgg aagcgatcac cgaggcgtcg gtcgcgcccg cgacccggat
      361 cagctccggg atgttcgggc cttccatggt gccgtccacc tcgatcgggg tgaaaaggaa
      421 acggaccacg ccgcgacggg tcagttcggc cgctgcgtcg gcgacgtcga ccccgctcga
      481 ttcggtccag ccggcgagcg acacctggcc gccgcgggcg tcgatgccga caaccacctt
      541 ttcggcgccg cgctcggcga tcagcgcgtc gaggaattcc cggtcacgca ttgcggcggt
      601 tccgaccaca acccgggccg cccccgcgtc gaggatccgt tcggccgtct cgagatcccg
      661 tattccgcca ccgacctcga tcgggcagtc gaccgcagcg gcgatacttt cgatccggcc
      721 gaggttcacc ggctcaccgg tcttcgcccc gtcgagatcg accacgtgga tgtactctgc
      781 cccgccatcg gcccactgcc gggccgcgtc ggcaggatca tcgaagtacc gggtctcctg
      841 gtcgtattcg cccttcagca gccgcaccgc cttgccgtcg aggatgtcga tcgccgggta
      901 gaggatcacg agcagatctc cctgaaattg cgcagcatcc ggagccccgc ggcgctggat
      961 ttctcgggat ggaactgggc gccccagacc ttgccccggc cggccataca ggcgaaacgg
     1021 ccgccatagg aggcagtgcc gatcagctcg gaatcggtcg gctgaaccac gaaggaatga
     1081 acgaagtaga agggctcccc gggccggagt ccctcggtca gcggatccgg cttggcccag
     1141 gtgacctccg cccagccaat gtgcgggatc ttccgaccac cggccgggac ctggacgacg
     1201 tcgccctcga gcaggccaag gccttccgcc ccgccctgct cgaccgaaga ttcgaagagc
     1261 agctgaaggc caaggcagat ccccagcacc ggcttgtcct ccgccaccgc atcgcgcagc
     1321 atgcggtcga gttcgagttc gcggatcttt tccatcgcct tcggaaacgc gccaactcct
     1381 ggcagcacga gtccgtcggc ggagtcgatc tccacctcat cggcggtcac gattgccctt
     1441 gcccccacct tctcgagggc cttctcgacc gagcgaaggt tgcccatccc gtaatcgaga
     1501 atggcgattt tcgggtcagc gcccacggcc aggccccctg tcgccggtca accgttcagc
     1561 gtgcccttgg tgctgggcac gcctttctcg tccgggtcga tcgagaccgc gacccgtagt
     1621 gcccgggcga atgccttgaa ggccgcctcg atcatgtggt gcacattcga tccgtaccgg
     1681 atgtcgatgt ggagggtcat cttcgctccg cccgcgacgg cgcggaaaaa ctcttcggtc
     1741 aactcggtct cgtagttggc gatcgaggtg accgggatat cgccacggaa gaccgaaagc
     1801 gcacggccag aaaagtcgag ggcgcattcg gcgcaggcct cgtccatcgg caccacagca
     1861 ctgccgtagc gcctgattcc tgcccggtca ccgagggcct ggtcgatcgc ctgcccaatc
     1921 acgatgccga cgtcttcgac cgtgtggtgc gacccggtct cgaggtcacc ggtcgccttc
     1981 acgtcaaggc cgatgttggc gtgacgcgca agcagatcca gcatgtggtc gaagaatccg
     2041 acacccgttt cggcgctgta ttcgccaccg tcgaggtcga gcgcgagctc gatgctcgtc
     2101 tctccggtct ttcgctcgat tcttgcctgt ctgctcatga cttttcctgg tcgtgtttct
     2161 gggcccttgc tgtcgccgac agcccgtgga ccggcaaccc ttcggccctg gcgagggctt
     2221 ccagcgggcc ggccagcctg cgggccgaat cggcgtccaa gcttacccgc gccgttcccc
     2281 ggcggaacgt ggcgggagaa agcgggccaa aaatcctgcc ggtgccatcg gtgggcagca
     2341 cgtggttgga accggcgacg taatcgccga aggcggtccc tgcctcctgc ccggtgaaga
     2401 cgcagcccgc ggtccgcacc gcgccggcca gtgccgccga atcttcgctc atcagttcca
     2461 ggtg
//
LOCUS       DAOTIP010000289         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50519_length_2464_cov_3.079701, whole genome shotgun sequence.
ACCESSION   DAOTIP010000289 DAOTIP010000000
VERSION     DAOTIP010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50519_length_2464_cov_3.079701"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5138_06205"
     CDS             <1..363
                     /locus_tag="P5138_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRV60204.1"
                     /translation="AVSEIFATLEEFPDLTVVLHCFSAPAWVERAAERDWYCSFAGNV
                     TYPANEDLREAAARVPGDRILVETDAPYLTPQSRRRDRNQPAFVVETAEKVAEVRGVS
                     YGELENTVTANAARLFGW"
     gene            360..1127
                     /gene="rsmA"
                     /locus_tag="P5138_06210"
     CDS             360..1127
                     /gene="rsmA"
                     /locus_tag="P5138_06210"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRV60205.1"
                     /translation="MKLGQNFLADPNLLDAIVRDAGLAPDDIVLEVGAGEGVLTERLA
                     DVVSRVHVIEIDRGLEPGLAAVMSRPNVDLIWADAVRYDLAELDPAPTRMVANLPYAV
                     ATPVILRSAFELPSIESWTVMVQKEIGDRLRAAPGSKSYGAPSVLIQLTGEVRQLRKV
                     SRTVFKPQPRVDSAILAIRRTGPGPDEATRKLVRAGFAHRRKAMARSVDMVIPGSLAM
                     IREGLESAGIDAGIRAEALSPGQFAKLSGMIDLPSDD"
     gene            1120..1986
                     /gene="ispE"
                     /locus_tag="P5138_06215"
     CDS             1120..1986
                     /gene="ispE"
                     /locus_tag="P5138_06215"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRV60206.1"
                     /translation="MTDLGLPLELPAPAKLNLCLYLGPRRKDGLHELTSIFEPLTLGD
                     TITVAEGAPDALVDQVICPGIPGENLVTKAMRMMRSEGWSPPLLKVEIEKRIPVQAGL
                     GGGSADAAAILRLGVGEVDDLAGLALLLGADVPSQLSPKPLLVEGVGQKLTTLPEPAE
                     HWVVLLPFETGLSTRAVFDEADRMKLGRSQEEIDAISGRLWAVATNAVSPLTYPALLV
                     NDLEPAAISLKPEIGLARERLESAGAGFTGMTGTGPTVFGLFPDRESAGSAAAEVGPE
                     AIVCRGGVTEDP"
     gene            1989..>2464
                     /locus_tag="P5138_06220"
     CDS             1989..>2464
                     /locus_tag="P5138_06220"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRV60207.1"
                     /translation="MISVPETRKGRRLALAGIVVAALVVYFLYRKFFPEIDLQQLLDD
                     FANFLGAWTYLVVGLLAFLETGAFVGLLVPGETAMLVGGAVAGLGVINVFLLIAIAWV
                     MAFLGDSTSFWLGHRLGREFILKHGPKFGISVERYEQVEEYFEKHGGKTVLIGRFIG"
BASE COUNT          394 a          826 c          828 g          416 t
ORIGIN      
        1 gcggtctcgg agatcttcgc gaccctggag gagtttcccg accttaccgt ggtcctccat
       61 tgcttttcgg ctccggcatg ggtggagcgg gcggccgaaa gggactggta ctgctcattc
      121 gccgggaacg tcacctatcc cgccaacgag gacctgaggg aggccgcggc cagggttccg
      181 ggggaccgca tcctggtcga gaccgacgct ccctacctga ccccccagtc ccgtcggcgc
      241 gaccggaacc agcccgcctt cgtggtcgag actgcggaga aggtggccga ggttaggggt
      301 gtgagctacg gcgagctgga aaacaccgta accgccaacg ctgcccgcct gttcggatgg
      361 tgaagctcgg ccagaacttc ctggccgacc cgaacctgct ggacgcgatc gtccgtgacg
      421 ccggtctcgc tcccgatgac atcgtgctcg aggtcggcgc gggtgaaggc gtgctgaccg
      481 agaggctcgc ggacgtcgtc tcaagggtcc atgtgatcga aatcgatcgc ggcctcgagc
      541 cgggactggc ggcggtgatg tccaggccga acgtggacct gatctgggcc gacgcggtca
      601 ggtatgacct ggcggagctc gacccggccc cgacccggat ggtcgcgaac cttccctatg
      661 cggtggcgac cccggtcatc cttcgcagcg ccttcgagtt gccctcaatc gagagctgga
      721 cggtgatggt ccagaaggag atcggagacc ggctccgggc cgcgccggga agcaagtcct
      781 acggcgcccc ttcggtcctg atccagctga ccggggaggt gcgccagctg cgaaaggtga
      841 gccgcacggt cttcaagccc cagcctcgcg tcgattcggc gatccttgcg atccggcgca
      901 ccggcccggg accggacgag gcaacccgga agctggtccg ggcagggttc gcccaccgcc
      961 gcaaggccat ggcccgttcg gtggacatgg tcatcccggg cagcctcgcc atgatccggg
     1021 aaggactgga atcggccggg atcgatgccg ggatccgggc ggaggcgctt tcccccggcc
     1081 agttcgcaaa gctttcaggg atgatcgacc tcccctccga tgactgacct cggccttccg
     1141 ctcgagctcc cggcaccggc gaagctgaac ctctgccttt atctcggtcc gcgccgcaag
     1201 gacgggctgc atgagctgac ctcgatcttc gagccgctga ccctcggcga cacgatcacc
     1261 gtcgcggaag gcgccccgga cgccctcgtc gaccaggtga tctgcccggg aataccgggc
     1321 gagaacctcg tgaccaaggc gatgcggatg atgcgctcgg agggctggtc gccgccgctg
     1381 ctcaaggtgg agatcgaaaa gaggatcccg gtccaggccg gtctgggcgg cggcagtgcc
     1441 gatgccgcgg caattctccg gctgggagtc ggggaggttg acgacctggc cggcctggcc
     1501 ctgctgctcg gcgccgacgt gccatcgcag ctcagcccga agccactgct ggtcgagggc
     1561 gtcgggcaaa agctgaccac cctccccgag ccggccgaac actgggtcgt tctgctgccc
     1621 ttcgagaccg gcctttcgac ccgggcggta ttcgacgagg ccgaccgcat gaagctgggc
     1681 cgcagccagg aggagatcga cgcgatctcc ggtcgcctct gggcggtggc caccaacgct
     1741 gtttcgccgc tcacctatcc ggccctgctg gtcaacgacc tggaacccgc cgcgatctcg
     1801 ctgaaaccgg agatcggact ggccagggaa cggctcgagt cggccggcgc cggattcacc
     1861 ggcatgaccg gtaccggacc cacggtcttc ggcctcttcc ccgaccggga gtcggccggg
     1921 tcggcggcgg cagaggtggg ccctgaagca atcgtctgcc ggggcggcgt caccgaagac
     1981 ccctgaggat gatcagcgtt cccgaaacca ggaagggcag acgcctggct ctggcgggca
     2041 tcgtggtcgc cgccctggtc gtctacttcc tctaccggaa gttcttcccg gagatcgacc
     2101 tgcagcagct gctcgacgat ttcgcgaact ttctcggcgc ctggacctac ctcgtggtcg
     2161 gactgctcgc cttcctcgag accggcgcct tcgtcggcct gctggtgccc ggagagaccg
     2221 ccatgctggt cggcggcgca gtcgccggcc tcggcgtcat caacgtcttc ctgctgatcg
     2281 cgattgcctg ggtgatggca ttcttgggcg actcgaccag tttctggctc ggccaccggc
     2341 tcgggcgcga attcatcctc aaacatggcc cgaaattcgg catttcggtc gagcgttacg
     2401 aacaggtcga ggaatacttc gaaaagcacg gcggcaagac cgtcctgatc ggccgtttca
     2461 tcgg
//
LOCUS       DAOTIP010000290         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50529_length_2463_cov_3.578904, whole genome shotgun sequence.
ACCESSION   DAOTIP010000290 DAOTIP010000000
VERSION     DAOTIP010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50529_length_2463_cov_3.578904"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="P5138_06225"
     CDS             complement(<1..692)
                     /locus_tag="P5138_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRV60208.1"
                     /translation="MRPLAGAVMLKRIITIALLIVAIVAVVLLIRSCNDDSDAYEIRA
                     VFDNGAFVVPGIDVRVAGANVGSVSSVDVSRPGEETGTDVPGKAIVVMNITDKGFQDW
                     KDDASCIIRPQSLIGEKFLDCQVTEPRPAGSEPPPSLEKITDGQPGAGEYLLPIENNA
                     QSVDLDLINNIYRLPYAQRFRIILNELGAGLATRGEDLRETVERANPALLQVNKVLAI
                     LASENKRLAKLAV"
     gene            complement(668..1987)
                     /locus_tag="P5138_06230"
     CDS             complement(668..1987)
                     /locus_tag="P5138_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRV60209.1"
                     /translation="MLRVVTLGALVVAIVFVASLIFKGDSGYKYSFDFQSASGMVTGN
                     FVMVGGSPIGKVTSVELTENNRVKMDVEIDRELHEGTSAVIRKSSLSSLHNHYISVQP
                     GPDNEPVLPEGHDFGEGVTQTAVELDQFFDTFDETTRKGWSNWIQGIAAIYAGKGAEG
                     ANKTFKYSGAAFSSTQRLMAELADQDTHLNQFVGNTSKFVTNLAEVAPQLTELVSNTN
                     TALGAIARNNESLALALNELPPTLRQGNTTFANLRAALNDVQPMVAASGRAADAGLAQ
                     YLKNDLRPVLNRARPVFNNLATVAYRPGPNNDTSDLESQLIPLHGKAQPAVNALVRAL
                     NANQEEVSEARAYSPDIFGSWAKLAGILGAYDANGPYARVTPTGTGVFQLNGTAIQPA
                     DTAVYSGLDVITGVNRCPGGGVQALAGSNPFLDNGNLTGKCDPSQVP"
     gene            complement(2133..>2463)
                     /locus_tag="P5138_06235"
     CDS             complement(2133..>2463)
                     /locus_tag="P5138_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60210.1"
                     /translation="VTPFVAEGRWPGVDGGWGIDPITSFPYDGSAIAYWDSGPLRPGP
                     GPDGMIGTDPPPITNTAPAAGEDPHEFPRVSGTAVEMIDRFLRDGGAVTNPCAPGPCL
                     AGGWTGS"
BASE COUNT          412 a          767 c          817 g          467 t
ORIGIN      
        1 acagcgagct tcgccagacg cttgttctcg gaggcgagga tggccagcac cttgttgacc
       61 tggagcagag ccgggttggc ccgctcgacc gtctcgcgca ggtcctcgcc cctggtcgcc
      121 aaaccggcgc cgagttcgtt gaggatgatc cggaaccgct gcgcgtaggg cagacggtag
      181 atgttgttga tcaggtcgag atcaaccgac tgggcgttgt tctcaatcgg caacaggtac
      241 tcgccggcac cgggctggcc gtcggtgatc ttctccagcg aaggcggcgg ctcggacccg
      301 gccgggcgcg gctcggtgac ctgacagtca aggaacttct cgccaataag tgattgcgga
      361 cggatgatgc aggatgcgtc gtccttccag tcctggaaac ctttgtcggt gatgttcatc
      421 accacgatcg ccttgccggg aacgtcggtg cccgtttcct ctcccggcct ggaaacgtca
      481 accgagctga cgctgcccac attggccccg gctacgcgga cgtcgatgcc gggcaccacg
      541 aacgccccgt tgtcgaagac cgcccggatc tcataggcgt cgctgtcgtc gttgcagctc
      601 ctgatcagca ggaccaccgc gacgatcgcg acgatcagta gggcgattgt gatgatccgc
      661 ttcagcatca cggcacctgc gaggggtcgc atttgccggt caggttgccg ttgtccagga
      721 atgggttcga gcctgccagg gcctggacgc cgccaccggg acagcggttg acgccggtga
      781 tcacgtctag cccgctgtag accgccgtgt cagccggctg gatcgccgtg ccgttcagct
      841 ggaatacgcc ggtgccggtc ggagtcacac gcgcgtacgg gccgttggcg tcataggcgc
      901 cgaggatccc ggccagcttg gcccaggaac cgaagatgtc cggcgaatag gccctggctt
      961 cggaaacctc ttcctgattg gcgttgaggg ctcgaaccag ggcgttcacg gccggctgcg
     1021 ccttgccgtg aagcgggatc agttgggact cgaggtcgga agtgtcgttg ttgggtccgg
     1081 gacggtaggc aacggtcgcc agattgttga aaacgggcct tgcccggttc aacaccggcc
     1141 gcaggtcgtt cttcaggtac tgcgcgagac cggcatcggc cgcccgcccg gaggcagcga
     1201 ccatcggctg gacgtcgttc agcgcggccc gcagattggc gaaggtcgtg ttgccctggc
     1261 gcagggtcgg tggcagctcg ttcagggcaa gggccaggga ctcgttgttg cgcgctatcg
     1321 cccccagtgc cgtgttcgtg ttgctgacca gttcggtcaa ctgcggagca acctccgcga
     1381 ggttggtcac gaacttcgag gtgttgccga cgaactggtt gaggtgagtg tcctgatcgg
     1441 ccagttcggc catcagccgc tgggtgctgg aaaaggccgc cccgctgtat ttgaaggtct
     1501 tgttggctcc ctcggcgccc ttgcccgcgt agatcgcggc gatgccctgg atccagttcg
     1561 accatccctt gcgggtcgtc tcatcgaagg tgtcgaagaa ctggtcgagc tcgaccgcag
     1621 tctgggtcac gccttcgccg aagtcatgcc cctccggaag gaccggctcg ttgtccggac
     1681 cgggctggac cgagatgtag tgattgtgaa gcgaggagag cgagctcttg cggatcaccg
     1741 ccgaggtgcc ttcgtgaagc tcgcggtcga tctcgacgtc catcttcacg cggttgttct
     1801 cggtcagctc gaccgaggtg accttgccga tcggcgagcc cccgaccatg acgaaattgc
     1861 cggtaaccat gccgcttgcg ctctggaagt cgaacgaata cttgtagccc gaatctccct
     1921 tgaagatcag cgatgcgacg aacacgatcg caacaacaag cgcgcccaga gtcacaactc
     1981 tgagcacggt tgagctacgt ccgcgattga cgcgacttgg ttttgcttga ttggcagtcg
     2041 tcaatctcag ctacccggtg attctcccca cctaagaagc ctccttcctg tgcggggcat
     2101 gctacctgag cgcccggcgt aagcgcctca cgtcaggagc cggtccagcc cccggcgagg
     2161 caggggccgg gagcacaggg atttgttacc gctccgccat cgcgcaggaa gcggtcgatc
     2221 atctccaccg cggtgccgga aactcgcggg aactcatgcg gatcctcgcc cgcggcagga
     2281 gccgtgttgg tgatcggggg cgggtcggtt ccgatcatcc cgtccgggcc gggacccgga
     2341 cgcaacgggc cgctgtccca gtaggcgatc gcggaaccgt cgtaagggaa gctggtgatc
     2401 gggtcgatgc cccagccacc gtccaccccg ggccagcggc cctcggcgac gaagggagtg
     2461 acg
//
LOCUS       DAOTIP010000291         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50687_length_2458_cov_3.714107, whole genome shotgun sequence.
ACCESSION   DAOTIP010000291 DAOTIP010000000
VERSION     DAOTIP010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50687_length_2458_cov_3.714107"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="P5138_06240"
     CDS             <1..540
                     /locus_tag="P5138_06240"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRV60211.1"
                     /translation="NPIGPRGVYDEAKRYAEALTMAYHRQQGVDTSIVRIFNTYGSRM
                     RAHDGRAIPTFLRQALQNQPLTVFGDGSQTRSFCFVDDLVRGLILLAESGEHLPVNIG
                     NPDEYTLLQLANAVIEASGAESSVTFEPLPTDDPQVRQPNIDRAKELLGWEPTVPLSE
                     GLRKTIDQSGRDRLIGKTG"
     gene            550..1437
                     /locus_tag="P5138_06245"
     CDS             550..1437
                     /locus_tag="P5138_06245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV60212.1"
                     /translation="MTGAGTVLVLGATGFLGGRIVDEFEAAGVEVAAASRPEGDLLDP
                     AGLRELIARTAPDTVVNAAGMTSPAIARERPADCFAVNTGGMLNLLEAVRLEAPGARV
                     LALSSAAVYAGEPPFTENSPTAATTPYAASKIAMEILCGQYSRGHGMTVTVLRCFNLT
                     GPGEPASQATSEFARAAIEAGAGGRAEVRVGEPATARDFTDVRDAARAIRLLAEGEHD
                     GTFDLCSGSATSLQELAGMISELSGAELSLRGSGTGQPASGLLSVRGDASKLALATGW
                     KPEIGLRESLADLLDSLGA"
     gene            1449..2069
                     /locus_tag="P5138_06250"
     CDS             1449..2069
                     /locus_tag="P5138_06250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60213.1"
                     /translation="MEASGSTEAVDTTNYEKFQTGNPVVQRLIGGFYRKVEDIVRPLK
                     PATVLDAGCGEGETLDRLAGVLPPFPVGIDLNQESVDFAAERLPDATIRRADLLELPF
                     EDDSFDLVFCLEVLEHLPDPGAGLEQLARVSRQDIVVSVPHEPWFRLGSLARGKYLKT
                     WGNHPEHVNHWNPKSFRSFLSTRTEVVDVQTAMPWIIARCRPLPAG"
     gene            complement(2035..>2458)
                     /locus_tag="P5138_06255"
     CDS             complement(2035..>2458)
                     /locus_tag="P5138_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60214.1"
                     /translation="IPVNHRPRLHGSSRFGLERYRRGALDLLTVAFLGRYRNRPLHLF
                     GLLGIGLGFLGFVICLYLSIIWIGGEPIGSRPLLFLGVLMIVVGVQLFSLGLIGQMLV
                     AGRRAGVSEERALIEKVVSPAIDPGLDLSRLEEAGTGR"
BASE COUNT          428 a          836 c          838 g          356 t
ORIGIN      
        1 aatccgattg gcccgcgcgg cgtctacgac gaggcgaagc gttacgccga ggcgttgacc
       61 atggcctacc accgccagca gggcgtggac acctcgatcg tccggatctt caatacctac
      121 ggttcgagga tgcgcgccca cgacggccgg gcgatcccga ccttcctgcg ccaggcgctc
      181 cagaaccagc cgctgaccgt tttcggcgac ggttcgcaga cccgttcttt ctgcttcgtc
      241 gacgacctgg tccgggggct gatcctgctg gccgagtcgg gcgagcacct tcccgtcaac
      301 atcggcaacc cggacgagta caccctgctg cagctcgcca acgcggtgat cgaagcctcg
      361 ggggccgagt ccagcgtgac cttcgagccg ctgccgaccg atgacccgca ggtccgccag
      421 cccaacatcg accgggcgaa ggagctgctc ggctgggagc cgaccgtgcc gctttcggag
      481 ggcctccgga agacgatcga ccagtccggt cgcgaccgcc tgatcggcaa gaccggctga
      541 acgcggcaga tgaccggggc cgggacagtc ctcgtcctcg gggccaccgg cttcctcggc
      601 ggccggatcg tcgatgaatt cgaggccgcc ggagtcgaag tggcggcggc ctcccggccg
      661 gagggcgatc tgctcgatcc agccggcctg cgggagctga tcgcccggac cgccccggac
      721 acggtcgtca atgcggcagg catgacctcg ccggccatcg ccagggaacg accggccgac
      781 tgcttcgccg tcaacacggg cggcatgctc aacctgctgg aggcggtccg gctggaggca
      841 cccggggcgc gggtcctggc tctttcatcc gccgccgtct atgcgggtga gccgccgttt
      901 accgaaaact ccccgaccgc tgccacgacg ccctacgccg cctcgaagat agcgatggag
      961 atcctctgcg gtcagtactc ccggggccat ggcatgacgg tcacggtgct gcgctgcttc
     1021 aacctgaccg gtcccggcga gcctgccagc caggcgacat cggaattcgc gcgggccgcg
     1081 atcgaggcag gagcgggagg cagggcggag gtcagggtcg gcgaaccggc gacggcccgg
     1141 gacttcaccg acgtgaggga cgccgcccgg gcgatacgcc tcctcgccga aggggaacac
     1201 gacggaacct tcgacctctg ctccggcagc gcgaccagcc tgcaggaact cgcgggaatg
     1261 atcagcgaac tgtccggggc tgaactgagc ctgcgcggct cgggcacagg ccagccggca
     1321 tcgggccttc tcagtgtccg cggggacgcc tcgaaactgg cattggcgac aggatggaag
     1381 ccggagattg gactccgtga gagcctcgcc gacctgctcg actcgctcgg cgcataggat
     1441 cccgcgccat ggaagcaagc ggttcaaccg aggcggtgga caccaccaac tacgagaagt
     1501 tccagaccgg caacccggtg gtgcagcggc tgatcggcgg cttctaccgg aaggtcgaag
     1561 acatcgtaag gccgctaaaa ccggccaccg tgctcgatgc cggctgcggt gaaggcgaga
     1621 ccctcgaccg tctggccgga gtcctcccgc cctttccggt cggcatcgac ctcaatcagg
     1681 aatcggtcga tttcgccgca gagaggctgc cggacgcgac gatccgccgg gccgatctgc
     1741 tggagctgcc cttcgaggac gacagcttcg acctggtctt ctgcctcgag gtgctcgagc
     1801 acctgcccga tcccggggcg ggcctggagc agctggccag ggtctcccga caggacatcg
     1861 tcgtttcggt tccgcatgag ccctggttcc ggctcggcag cctggcccgg ggcaagtatc
     1921 tgaagacctg gggcaaccat cccgagcacg tcaatcactg gaacccgaag agcttccggt
     1981 ccttcctttc cacccggacc gaggtggtcg acgttcagac cgcgatgccg tggatcatcg
     2041 cccggtgccg gcctcttcca gccggctgag gtcgaggccc ggatcgatgg ccgggctgac
     2101 caccttctcg atcagggccc gctcctccga aaccccggcc cggcgaccgg cgaccagcat
     2161 ctggccgatc aggccgagcg agaagagctg gacgccgacc acgatcatca gcacgccgag
     2221 gaagagcagc ggccggctgc cgatcggttc gccgccgatc cagatgatcg acaggtaaag
     2281 gcagatcacg aagccgagga agccgaggcc gatgccgagc agaccgaaga gatggagtgg
     2341 ccggttccgg taccggccga gaaaggcgac ggtgagcagg tcgagggcac cgcggcggta
     2401 acgctccagg ccgaagcggg atgagccgtg gaggcggggg cgatggttga ccgggatc
//
LOCUS       DAOTIP010000292         2455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50774_length_2455_cov_2.765000, whole genome shotgun sequence.
ACCESSION   DAOTIP010000292 DAOTIP010000000
VERSION     DAOTIP010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50774_length_2455_cov_2.765000"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1405
                     /locus_tag="P5138_06260"
     CDS             65..1405
                     /locus_tag="P5138_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRV60215.1"
                     /translation="MTSFDFEGLAGHGQSELRGKALGIAAAGLTAADGADAVRRTVRE
                     VAEGVEIGGNLYRLDPGSRLIVLGGGKATMPVALALEEILGDRIDAGAVVLQRGRKAG
                     LDRLEVMYADHPLPSPDSVLAAERMVELAGEARQGDLFITCFTGGRSALLCLPPSEVG
                     FEDKRRLNELLLASGMPITAINTVRKHVSRIKGGRLAARIAPARIVNLTVSDVAGDPL
                     DVLADLTTPDSSTPGDAINVLGNYGLLGKVPASVAMHLESEQAASPLLDGQPIETVML
                     VNGESVCEAMAGAALNRGLDPVIVGTCLEGEAVEVGEQLARLAVESPPGTALIGCGGE
                     STVTLGESAFGAGGPNQEVALAAARSLGGHAAAGVFLDTDGSDGGSTSAGATCDGETA
                     ERAHEADLDLDRALAAHASSEPLSALGDLVETGPTGTNVNDLFVIVTGEGSTGK"
     gene            1416..1742
                     /locus_tag="P5138_06265"
     CDS             1416..1742
                     /locus_tag="P5138_06265"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV60216.1"
                     /translation="MKAHSVIDQLEVDPLDPDQVVSGEPETSELTLLETDTDGTSGLW
                     QLTPGVVTDTEVEESFLVLSGRGEVTFEDGSRILLEPGVTHRFSGGEQTTWTVEETLL
                     KAYWIA"
     gene            complement(1769..2077)
                     /locus_tag="P5138_06270"
     CDS             complement(1769..2077)
                     /locus_tag="P5138_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60217.1"
                     /translation="MSAPDPIEDGERDSVEPEPVEESPVPARDEKFGLNLGNPLESEG
                     AAFSWLIVVLIAAVSIGAAAKIISPLAAVIWTIILLGVVSVPIFRGLKHQLGSPEDDE
                     "
     gene            complement(2080..>2455)
                     /locus_tag="P5138_06275"
     CDS             complement(2080..>2455)
                     /locus_tag="P5138_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60218.1"
                     /translation="ACGGDKSDDNAKQFRDQVAKLEAGVRLDPVDADQPGVVSGSVPT
                     APGGGLDFTFSFGPDADNLDGQFPDRDEINHFTGGDEYYSSFSNSPSGLNLRQQNERL
                     DLELALSDVACQAAAAKDCERP"
BASE COUNT          440 a          802 c          819 g          394 t
ORIGIN      
        1 ggacccggca gttccgttga ccacaccgtc cggagcctga tccgggccgg accggagccc
       61 gcccgtgacc tcctttgact tcgaaggact cgccgggcac gggcagtccg aactgagggg
      121 taaggccctc gggatcgccg ctgcgggcct gaccgcggca gacggcgctg acgccgtgcg
      181 aaggaccgtc agagaagtgg ctgagggggt cgagatcggt gggaacctct accgcctgga
      241 cccggggagc cggctcatcg tcctcggagg tggcaaggcg accatgccgg tcgcccttgc
      301 cctcgaggaa atcctcggcg accgcatcga cgccggcgca gtggtactcc agcgcggccg
      361 gaaagccggg ctcgaccggc tcgaggtgat gtacgccgac caccccctgc cctcccccga
      421 ctcggtactg gctgccgaaa ggatggtcga actggcagga gaggccagac agggggatct
      481 gttcatcacc tgtttcacag gtggacgttc agccctgctc tgcctgcccc cgtcggaagt
      541 cggtttcgag gacaagcgtc ggctcaacga gttgctgctg gcttccggga tgccgatcac
      601 cgcaatcaac acggtccgca agcatgtctc ccggatcaag ggcgggcgcc tcgccgcaag
      661 gatcgcgcct gcgcggatcg tcaacctgac cgtctccgac gtggccggcg atccacttga
      721 cgtgctcgcc gacctcacaa cccctgattc ttcgactccg ggggacgcga tcaacgtcct
      781 cggcaactac ggactgctcg gcaaggttcc ggcgagcgtg gccatgcacc tcgaatcgga
      841 gcaggctgct tccccacttc tggacgggca gccgatcgaa acggtgatgc tggtcaacgg
      901 cgagagcgtc tgcgaggcga tggcgggggc agctctaaac agggggctcg atccggtgat
      961 cgtcggcacc tgcctcgaag gcgaggcggt ggaagtcggc gaacagctcg cccgcctcgc
     1021 cgtcgagagt cctcccggca cagcactgat cggttgcggg ggcgaaagca cagtcacgct
     1081 cggcgagtcg gcgttcggtg ccggcggccc caatcaggaa gtcgccctgg cggcagcgag
     1141 atcgctggga gggcatgctg cggccggggt attcctcgac acggatggat cggacggagg
     1201 cagcaccagc gccggcgcca cctgcgacgg agagaccgct gagagagctc atgaggccga
     1261 tctggacctg gaccgcgccc tggccgctca cgcctccagc gaacctctca gcgcccttgg
     1321 agacctggtt gagacgggcc ccaccggaac caatgtcaat gacctgttcg tcatagtcac
     1381 cggcgaaggt tcaaccggaa agtgaaggag gagcaatgaa ggcccattcc gtcatcgacc
     1441 agcttgaggt cgatcccctg gatccggatc aggtcgtctc aggtgagccc gagacctcgg
     1501 agctgaccct gctcgaaacg gataccgacg ggacctccgg cctctggcag ctgacccccg
     1561 gggtcgtgac cgataccgag gtcgaggagt cctttctcgt cctcagcggc cgtggcgagg
     1621 tcaccttcga ggacggaagc cggatcctgc tggaacccgg agtgacgcac cgcttcagcg
     1681 gcggagagca gaccacctgg acggtcgagg agacgctcct caaggcctac tggatcgcct
     1741 gaacgggccc gtagcgctcc ggggaggcct actcgtcgtc ttccggactg cccagctgat
     1801 gcttcagccc gcggaagatc ggcacgctga cgacccccaa caggatgatc gtccagatca
     1861 ctgcggccag tggcgagatg atcttggccg cagccccgat tgaaacggcg gcgatcagca
     1921 ccacgatcag ccagctgaat gccgcgccct cggactccag gggattgccc aggttcagtc
     1981 cgaacttctc gtcgcgggcg ggaacgggcg attcttcgac cggctccggc tccacggagt
     2041 cccgctcgcc atcttcgatc ggatcgggtg cggacatggc tagggacgct cgcaatcctt
     2101 ggcggcggct gcctggcagg cgacgtcact cagcgccagc tcaaggtcga gccgctcgtt
     2161 ttgctggcga aggttgagcc cggaaggcga attgctgaat gaggagtagt actcgtcacc
     2221 gccggtgaaa tggttgatct catcccggtc ggggaactgg ccatcgaggt tatccgcgtc
     2281 ggggccgaag gagaaagtga aatcaagccc accacccggg gcagtgggga ccgatccgct
     2341 cacgacaccg ggctggtcgg catcaacggg gtcgagccgg accccggcct caagtttcgc
     2401 gacctgatcc cggaactgtt tggcgttgtc atcgctcttg tcgcccccgc aggca
//
LOCUS       DAOTIP010000293         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_50829_length_2453_cov_2.770225, whole genome shotgun sequence.
ACCESSION   DAOTIP010000293 DAOTIP010000000
VERSION     DAOTIP010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50829_length_2453_cov_2.770225"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1727
                     /locus_tag="P5138_06280"
     CDS             54..1727
                     /locus_tag="P5138_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRV60219.1"
                     /translation="MNRRSFEGFSGRFAVGGLITLAFFGLLMMVGGLHEKAWSAPRKH
                     LSNFGNRPNIVLIQTDDLVRGDIKYLPNVKRFLQRGGTTFTNYNTPYPLCGPARAALL
                     TGQLSHNNGVLANFRANDGGYYRLRDLPGKRNQSNTLGPWMKKAGYRTGFVGKYLNEY
                     STLDLTEIPPGWDSWKALLDQSTYDYYNYGMNLNGKVHYWGDKDYAKAQINLGTLSTV
                     NTPESFAELLATFHQAFDPWDYFGWQRAKDYTMDVNGKMANDFVKSSVKSRKPFFLYY
                     ATPGPHAEDTNNIQGLRPGAPQPDPRPPKRYMHTFDNVKAPRTPAFNEADVSDKAANV
                     KDLPLLTDEQIDRVDASFRGRLGAARSIDDQVGKIVKSLKRGHELNNTIIIFTSDNGY
                     LQGEHRLASSKFLPFENSIRVPMLMRGPGIKAGRKINSTTMDIDLTPTILKAARAKPG
                     RLMDGISLLAAARKKGKLPYRNLPIEAERSLFKFTTPLTKFDLPFYGVKTSRYKYVHW
                     SFGDIELYDMKKDPYELNNIASEPAMAGVVSKLEAKASQLSNCKGKACR"
     gene            1724..2122
                     /locus_tag="P5138_06285"
     CDS             1724..2122
                     /locus_tag="P5138_06285"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRV60220.1"
                     /translation="MIDPTPEQFKALVESDDESPIVMVNLIRFKDQATGIDEGMTGAE
                     AYATYGRNIAPYLSEVGGEVLISTNTVESVIGPEEAEWDAVLLVRYPSRKAFVAMITN
                     PGYQKEHEHRAAGVEEARLILSGLAFGGSA"
     gene            2119..>2453
                     /locus_tag="P5138_06290"
     CDS             2119..>2453
                     /locus_tag="P5138_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60221.1"
                     /translation="MSEEAASDPDPGQAGAPLAGVIEAGAYWPRPWSGEDGGPRRVCR
                     PEGQPGLDIQPGEKLKLEVFRDSFAADMLIRREPGELYALRHDMPKGDPLAGDCEGWV
                     EKLDPETSAA"
BASE COUNT          502 a          811 c          751 g          389 t
ORIGIN      
        1 gatttggtta ttctccgaat ggaggagcca caccgggagt ggagaggatt gggatgaacc
       61 gtcgtagttt cgaaggtttt tcaggcaggt tcgcagtcgg cggactgatc acacttgcct
      121 ttttcggtct gctgatgatg gtgggcgggc tgcatgaaaa ggcctggtcg gcaccgcgca
      181 agcatctctc gaatttcggc aaccggccga acatcgtgct gatccagacc gatgacctgg
      241 ttcgcggaga catcaagtac ctccccaacg tcaagcgctt ccttcaacgc ggcggcacga
      301 ccttcaccaa ctacaacacc ccctatccgc tctgcggtcc tgcccgggcg gcgctcctga
      361 ccggccagct ctcccacaac aacggcgtgc tggccaactt ccgggccaac gacggcggct
      421 actaccggct gcgcgacctg cctggcaaga ggaaccagag caataccctc ggcccctgga
      481 tgaagaaggc cggttaccgc accggcttcg tcggcaagta cctgaacgag tattcgacgc
      541 tcgacctgac cgagatcccg cccggctggg acagctggaa ggcgctgctc gaccagtcga
      601 cctacgacta ctacaactac gggatgaacc tcaacggcaa agttcactac tggggcgaca
      661 aggattacgc caaggcccag atcaacctcg gcaccctttc caccgtgaac accccggaat
      721 ccttcgccga gcttctcgcc accttccatc aggccttcga tccctgggac tacttcggct
      781 ggcagcgggc caaggactac acgatggacg tcaacggcaa gatggccaat gacttcgtga
      841 agagttcggt caagtcgcgc aagccgttct tcctctacta cgcgaccccg gggccgcatg
      901 ccgaggacac caacaacatc cagggcctga ggcccggcgc tccccagccc gacccccggc
      961 ctccgaagcg gtacatgcac accttcgaca acgtgaaggc gccgcggacc ccggccttca
     1021 acgaggctga cgtctccgac aaggcggcca acgtcaagga tctgcccctg ctgacggacg
     1081 agcagattga tcgggttgac gccagcttcc gcggccgtct cggtgccgcg aggtcgatcg
     1141 acgatcaggt cggcaagatc gtcaagtccc tgaagcgggg ccacgagctg aacaacacga
     1201 tcatcatctt cacctcggac aacggctacc tgcagggcga gcaccggctg gcgtcctcga
     1261 agttcctgcc cttcgagaac tcgatccggg tgccgatgct gatgcggggc cccgggatca
     1321 aggccggccg gaagatcaac tcgaccacca tggacatcga cctgaccccg accatcctca
     1381 aggcagccag ggcgaagccg ggccggctca tggacggcat ctcgctgctg gccgccgccc
     1441 gcaagaaggg caagctgccc taccggaacc tgccgatcga ggccgagcgg tcgctcttca
     1501 aattcacgac gccgctgacc aaattcgacc tccccttcta cggtgtgaag accagccggt
     1561 acaagtacgt ccactggtca ttcggtgaca tcgagctcta tgacatgaaa aaggatccgt
     1621 acgagttgaa caacatcgca agcgaaccgg ccatggccgg cgtggtctcg aagctggagg
     1681 ccaaggcctc acagctgagc aactgcaagg gcaaggcatg ccggtgatcg atccgacccc
     1741 ggaacagttc aaggccctgg tcgaatccga tgacgagtcg ccgatcgtga tggtcaacct
     1801 gatccgcttc aaggatcagg cgaccgggat cgacgaaggc atgaccgggg cggaggccta
     1861 cgccacctac ggccggaaca tcgcgcccta cctgtccgag gtgggcggcg aggtgctgat
     1921 ttcgaccaac acggtcgaga gcgtgatcgg tcccgaagag gcagagtggg atgcggtgct
     1981 gctggtccgc tatccgtccc gcaaggcctt cgtcgcgatg atcaccaacc ccggctacca
     2041 gaaggaacac gagcatcggg ccgccggcgt cgaagaggcg cgcctgatcc tctccggcct
     2101 cgccttcggc ggatcggctt gagcgaagaa gcggcgagcg acccggaccc cgggcaagcc
     2161 ggcgcccctc tggccggagt gatcgaggcc ggcgcctact ggccgcggcc ctggtccggc
     2221 gaagacggcg gtccgcgccg ggtctgtcgt cccgagggac agccgggact tgacatccag
     2281 ccgggcgaga agctgaagct ggaggtgttc cgtgacagct tcgcggccga catgctgatc
     2341 cgccgcgaac cgggcgagct ctacgcactg cggcatgaca tgccgaaggg cgatccgctg
     2401 gccggcgact gtgagggatg ggtcgagaag ctcgaccccg aaacgagcgc cgc
//
LOCUS       DAOTIP010000294         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51145_length_2442_cov_3.415165, whole genome shotgun sequence.
ACCESSION   DAOTIP010000294 DAOTIP010000000
VERSION     DAOTIP010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51145_length_2442_cov_3.415165"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="folP"
                     /locus_tag="P5138_06295"
     CDS             complement(<1..786)
                     /gene="folP"
                     /locus_tag="P5138_06295"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRV60222.1"
                     /translation="MSPVPETLRTAAGEINVADPVIVGVVNATPDSFSDRGDRSTAAS
                     IRHVEEHLANGAEVIEIGGESNVTNRPAVTPEEEISRILPVVSAAAEAGAVISIDTYK
                     PAVARAALEAGAAIVNDISGGGNLELMGAAAEAGAGVVLMHTVVEPKTQRWDETAYPE
                     GITAHQLSFFEQRLEALAGLGIDRDQVVIDPGPDFGKTPRQTVDSLKGLPELCGLGCP
                     VMLAVSRKDFIGAINHRRPSERGPGTFAALAFGLDCGGRLLRVH"
     gene            794..1660
                     /locus_tag="P5138_06300"
     CDS             794..1660
                     /locus_tag="P5138_06300"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HRV60223.1"
                     /translation="MLDTFDHRDFAAADISRNLRETVSVVIPTRNTAGTIAATVAELK
                     VLADDGLVEQILVVDADSPDGTASLARDAGAEVVSENELVEGHGPCRGKGDAMWRALA
                     AVTGDIVVYIDGDIADFGRHYVTGVTGPLTDPGKHFVKGRYQRPFRYGESEEPSGGGR
                     VTELAARPLLARLAPELLAFDQPLAGEVAARRELLEQIPFACGYGVEISMLIDVWDRV
                     GIEGMAQVELGTKRNAHQPLSALSGMSSEVIAAMAAALDRLGREDTGRLEMVEGSGPE
                     VTVRPAFATVSR"
     gene            1673..>2442
                     /locus_tag="P5138_06305"
     CDS             1673..>2442
                     /locus_tag="P5138_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRV60224.1"
                     /translation="MSLRCVYTDLDGTLLGAGASLFTDEEGNFSMSQARALEACSRAG
                     VEVVIMSGRRQVQVHEDARIIGQTSFIFEAGSAYSMDQEITLMTGDFEHEDGKTVVQQ
                     MEERGVPDLLFESFPDRFEYHEPWHLNREMSSLFRGDIDAAEANRILEENGHGDLRLL
                     DNGAIHRRMEGIEQAHCYHLVPKGVSKAGAVAAHARARGYALEETIAVGDSLEDLEVA
                     PSVGRFFVVADGPEKDPGVREALGRYPNATVTEARNGAG"
BASE COUNT          400 a          813 c          855 g          374 t
ORIGIN      
        1 gtggacccgc agcaggcggc cgccgcagtc gagcccgaag gccagggcgg cgaaggtccc
       61 gggcccccgc tcggaaggcc ggcggtggtt gatcgctccg atgaagtcct tgcgggagac
      121 cgccagcatc accggacagc cgagtccgca gagctcgggc agccccttca gcgagtcgac
      181 cgtctggcgg ggagtcttgc cgaagtcggg gcccggatcg atcaccacct ggtcgcggtc
      241 gatcccgagt ccggccaggg cctccaggcg ctgctcgaag aagctcagtt gatgggcggt
      301 gatcccttcc ggataggcgg tctcgtccca gcgctgggtc ttcggctcga ccacggtgtg
      361 catcagcacc acacccgccc cggcttccgc ggcggctccc atgagttcca ggttgccacc
      421 gccactgatg tcgttgacga tcgccgcacc cgcctccagc gccgcgcggg cgaccgcggg
      481 cttgtaggtg tcgatcgaga tcacggcgcc cgcttccgcc gccgcgctga ccaccgggag
      541 gatccgggag atctcctctt ccggggtgac ggcaggacga ttggtcacat tcgactcgcc
      601 accgatctcg atgacttcgg ccccgtttgc caggtgttct tccacgtgcc ggattgaagc
      661 cgcggtagag cggtcgccgc gatcggagaa ggagtcgggg gtggcattga ccacccccac
      721 gatcaccggg tcggccacgt tgatctcccc ggccgcggtg cgtagtgttt ccgggacagg
      781 actcacctga gcgatgctag acaccttcga ccaccgggac ttcgccgccg ccgacatcag
      841 ccgcaacctg cgggaaacgg tcagcgtggt gatcccgacc cggaacacgg ccgggacgat
      901 cgctgccacc gttgccgagt tgaaggttct ggccgatgac ggtctggtcg aacagatcct
      961 ggtcgtcgat gccgactccc ccgatggcac cgcctcactg gcccgcgacg cgggagccga
     1021 ggtcgtctcc gagaacgaac tggtcgaggg tcacggaccc tgtcgcggca agggggacgc
     1081 gatgtggcgc gcgctcgccg cagtgaccgg ggacatcgtc gtctacatag acggggacat
     1141 agcggacttc ggccgtcact acgtgaccgg ggtgacgggg ccgctgaccg acccgggaaa
     1201 gcacttcgtc aagggccgct accagcgccc gttccgctac ggggaaagcg aggaaccatc
     1261 cggcgggggc agggtcaccg agctcgccgc ccgtcccctg ctggcaaggc tcgccccgga
     1321 actgttggcc ttcgaccagc ccctggccgg cgaagtcgcc gcccgacggg aattgctgga
     1381 gcagattccc ttcgcctgcg gctacggggt ggagatctcg atgctgatcg acgtctggga
     1441 ccgggtcggc atcgaaggga tggcgcaggt ggaactcggg accaaacgga acgcccacca
     1501 gccgctgtcg gcgctcagcg gcatgtcctc cgaagtgatc gcggcgatgg cggcagccct
     1561 ggaccggctc ggccgcgagg acacagggcg gctcgagatg gtggaaggga gcggccccga
     1621 agtgacggtg cggcccgcct tcgccaccgt cagcagatag cgtgacgcgc tgatgagcct
     1681 gcgctgcgtc tataccgacc tcgacggcac cctgctcggg gccggggcct cccttttcac
     1741 cgacgaggaa gggaacttct cgatgtcaca ggcccgggcc ctggaggcct gttcccgggc
     1801 cggggtagag gtcgtgatca tgtccggccg ccgtcaggtc caggtccatg aggacgcccg
     1861 gatcatcggc cagacatctt tcatcttcga ggccggatcc gcctattcga tggaccagga
     1921 gatcaccctg atgaccggtg acttcgagca tgaggacggc aagacggtgg tccagcagat
     1981 ggaggagcgg ggcgttcccg acctgctgtt cgaatccttc ccggaccggt tcgagtacca
     2041 cgagccctgg cacctgaacc gggagatgtc ctcgctcttt cgcggtgaca tcgacgcggc
     2101 agaagcaaac cggattctcg aggagaacgg ccacggggac ctgcgtctgc tcgacaacgg
     2161 cgcgattcac cgcaggatgg aggggatcga gcaggcccac tgctaccacc tggtcccgaa
     2221 gggggtttcg aaggccggcg cggtggccgc ccacgccagg gcccgcggct acgcgctgga
     2281 ggagacgatc gcggtcgggg attcgctgga ggacctcgag gtcgcgccct cggtcggccg
     2341 cttcttcgtg gtcgccgacg gccccgagaa ggaccccggg gtgcgggagg cgctggggcg
     2401 ctacccgaac gcgaccgtga ccgaggcgag aaacggcgcc gg
//
LOCUS       DAOTIP010000295         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51217_length_2439_cov_3.471896, whole genome shotgun sequence.
ACCESSION   DAOTIP010000295 DAOTIP010000000
VERSION     DAOTIP010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51217_length_2439_cov_3.471896"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..370)
                     /locus_tag="P5138_06310"
     CDS             complement(23..370)
                     /locus_tag="P5138_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60225.1"
                     /translation="MTETVETSTSRRAKQRAMMIAVDQTGEATLSELGELLKTAGVAT
                     VGEVIQRRSEPDPDRYFGRGKLEEVKEEVGRTGANLVAAIRLGQRLGPEATVVTVMCD
                     TGIKYISTALYAA"
     gene            444..1448
                     /locus_tag="P5138_06315"
     CDS             444..1448
                     /locus_tag="P5138_06315"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV60226.1"
                     /translation="MKIFMTGATGFIGGATARHLVEQGHQLTCLVRDRSKATGLSDLG
                     CELVDGDLSSRPALAEQMKGHDALLHNAALYEVGIPKDRIPVLRKANVEGTANTLEAA
                     LEAGIPRVLYVSTCAVFGNTHGEVATEDFRRPDLDSPGGLEFTSVYEETKYEAHQIAL
                     NLIKTRHLPCVIAQPGGVYGPDDHSELGSTMHQFLDGKMPMVPFPDFGTGLTNVEDIA
                     AGLALILDKGEIGECYILNESNFTMRQVLEEVGEVADRKVPKRNMPTAVLKALRPFGP
                     LVGKIMDQPPNLAELISSADGVTFWADATKAKTELGWRPRDVKTGLRQTLEAEGRLPA
                     "
     gene            complement(1500..>2439)
                     /locus_tag="P5138_06320"
     CDS             complement(1500..>2439)
                     /locus_tag="P5138_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV60227.1"
                     /translation="QAFSDFYRDRFGVEIDPESEVIPAIGAKECIYNLCFAFLDPGDV
                     ALASDPGYPVYTGGPILAGAEAVLLPLKEELGFAPDLDAIDASTAERARLLFLNYPNN
                     PSGAIVPDGFFEKVVDFARENEILVVHDNAYSETTYDGYVAPSFLATPGAKEVGVEVF
                     SLSKGYNMTGWRVGAILGNADAISTYWRLKTNVDSGLFEAIQLAAVAALDGPSGPLEE
                     MNAIYARRRDLVVDTLRAIGVDVKSPKGTIYVWAPVPEGYTSTSFCEKVLEEAAVVIS
                     PGSMYGRSGEGYFRISLTTPDERLTEALERMREHLA"
BASE COUNT          437 a          820 c          769 g          413 t
ORIGIN      
        1 aggggcagga tatcggccgg cgctaggccg cgtaaagcgc ggtgctgata tacttgatgc
       61 cggtgtcgca catcaccgtc acgacggtgg cctccggtcc caggcgctgg ccaaggcgga
      121 tcgcggcgac caggttggcg ccggttcgcc ccacttcctc tttcacctct tccagcttgc
      181 cgcggccaaa atagcgatcc ggatcgggct cgctgcggcg ctggatcact tccccgaccg
      241 tcgccacgcc ggcggtcttg agcagctctc cgagctcgga aagggttgcc tcgccggtct
      301 ggtctacggc gatcatcatc gctcgctgct tggcccggcg gctggtcgaa gtctcgactg
      361 tttcggtcac tggcttcatc ctagaacccg tgggtcgccc gctcccttcc ggaagccgca
      421 ccgctcgcgg tacgctgcga cacatgaaga tcttcatgac gggtgcgacg ggtttcatcg
      481 gcggtgctac ggccaggcat ctggtcgagc agggccatca actgacctgc ctggtgcggg
      541 accggtccaa ggcgaccggc ctttcggatc tgggctgcga actggtcgat ggcgaccttt
      601 cctcgcgacc ggcgctggcc gagcagatga aaggccacga cgccctgctc cacaacgccg
      661 ccctgtacga agtcgggatc cccaaggaca ggatcccggt cctccgcaag gccaacgtcg
      721 aagggaccgc gaacacgctc gaggcggccc tcgaggcagg gatccccagg gtcctctacg
      781 tctccacctg cgccgtcttt ggcaacaccc acggcgaggt ggcaaccgaa gatttccgcc
      841 gccccgacct cgacagtccc ggcggtcttg aattcacctc ggtttacgag gaaacgaagt
      901 acgaggcgca ccagatcgcc ctcaacctga tcaagaccag gcatctgccc tgcgtcatag
      961 cccagcccgg cggggtctac ggcccggacg accattcaga gctcggctcc acaatgcacc
     1021 agttccttga cggcaagatg ccgatggtcc ccttccccga tttcggcacc gggctcacca
     1081 acgtcgagga catcgccgcg gggctcgcac tgatcctcga caagggcgaa atcggcgagt
     1141 gctacatcct caacgaaagc aacttcacca tgaggcaggt gctcgaggag gtcggtgaag
     1201 tcgccgaccg caaggtcccc aagcggaaca tgcctacggc cgtcctgaag gccctccgcc
     1261 ccttcggtcc gctggtcggg aagatcatgg accagccgcc caaccttgcc gagctgatca
     1321 gctccgccga cggggtcaca ttctgggccg acgccaccaa ggcgaagacc gagctcggct
     1381 ggcgcccgag ggatgtcaag accggactcc gccagaccct cgaagccgag gggcgactcc
     1441 ccgcctaggc caggtgctct ctcatccgca gggttaccgc ggcagtcgct tcggcgcgat
     1501 cacgcgaggt gctcgcgcat ccgctccagg gcctcggtca ggcgttcgtc cggggtcgtg
     1561 agcgagatcc ggaagtagcc ttcgccgctt cgcccgtaca tcgagccggg tgaaatgacc
     1621 acagccgctt cttcgagcac cttctcgcag aagctggtcg aggtgtagcc ctccggcacc
     1681 ggtgcccaga catagatcgt gcccttgggg cttttgacgt caacgccgat cgcccgcagc
     1741 gtgtcgacca cgaggtcacg acgacgggca tagatcgcgt tcatctcctc cagcggaccg
     1801 gacggaccgt caagtgccgc cacagcagcc agctgaatcg cctcgaagag gcccgagtcc
     1861 acgttggtct tgagccgcca gtaggtgctg atcgcgtcgg cgttgccgag gatcgccccg
     1921 acccgccagc cggtcatgtt gtagcccttc gacagggaaa acacctcgac gccgacctcc
     1981 ttggctccgg gggtcgcgag gaagctcggt gcgacgtagc cgtcgtaggt ggtctccgag
     2041 taggcgttgt cgtgaacgac caggatctcg ttttcacgag cgaaatcgac caccttctcg
     2101 aagaagccgt cgggaacaat cgccccggaa gggttgttcg ggtaattcag gaagagcagc
     2161 cgggcgcgct ccgcggtgct ggcgtcgatc gcatccaggt cgggggcgaa gccaagttcc
     2221 tccttgaggg gcagcagcac ggcctcggcg ccggccagga tcggtccacc ggtgtaaacc
     2281 gggtagccgg gatcggaggc cagggcgacg tcacccgggt cgaggaaggc aaagcagagg
     2341 ttgtagatgc attccttggc accgatcgcc ggaatcactt cgctttccgg gtcgatctcg
     2401 acaccgaagc ggtcccggta gaagtccgag aaggcctgc
//
LOCUS       DAOTIP010000296         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51379_length_2434_cov_3.015553, whole genome shotgun sequence.
ACCESSION   DAOTIP010000296 DAOTIP010000000
VERSION     DAOTIP010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51379_length_2434_cov_3.015553"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="P5138_06325"
     CDS             <1..638
                     /locus_tag="P5138_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60228.1"
                     /translation="AVRSKELKFDRSTAFWPVVFPKETTYGMAWSDDPDRVSGIELPP
                     DLSPGWTLDPLYGDRVRYWNGEEWDLVAAPAPGNLDLYKRIAAGEDVGEVPEAVSIHP
                     ANSKEPDPTVEMLGWTPAEGEDDDDGGQPEPKTRSVEAPAGDLGEDEVSASDLEMARE
                     IARFIAERAFEFDDHEARSWALTDFDVWAVLAGESAQLSFDPVVTDKTRRR"
     gene            1116..>2434
                     /locus_tag="P5138_06330"
     CDS             1116..>2434
                     /locus_tag="P5138_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRV60229.1"
                     /translation="MSSCDCGNSEAATGLSWREAAFRFGPLRNALLAGIALVVGLVLE
                     FTGASEVLAIAAFLVAIPIGAWYFTQEGWEELVEEHEIGVEALMLIAAAGCLVFGLFE
                     EAAALVFLYSLAEAIEELTFVRTRSAIRDLLDLAPKQARRIVNGREEMIPAEDLVVGD
                     LFMVRPGEALPTDGVLRSGNSTLDEAPVTGESMPVAKDPGQEVFAGSVNGTGSIEIEV
                     TRPYADNTLRRIVHLVEEAQGQKSKAQRFVDRFATRYSPAVLVGAALVAVVGGIVDGD
                     WQEWALRGVTVLVAGAPCALVMSVPVAAAAAITRAGREGILIKGGLQLEALGRVNAVA
                     FDKTGTLTRGKPEVTDVVALNDVAESKGLRIAASIEARSEHPLAAAVVAYARTAGIEP
                     GPAEEFSALVGHGATALVEGRTAWVGSPALAIERMGSGELPDIVEKL"
BASE COUNT          444 a          681 c          837 g          472 t
ORIGIN      
        1 cggcggtccg gtcgaaggag cttaagttcg acaggtccac ggcgttctgg ccggtcgtgt
       61 tcccgaagga gaccacctac gggatggcgt ggagtgatga ccctgatcgg gtctccggga
      121 tcgagctacc gccggatctg agcccgggtt ggacgcttga tccgctttac ggggatcggg
      181 tgaggtattg gaacggagag gagtgggact tggtcgctgc cccggctcct ggcaacctcg
      241 atctctacaa acgaatcgca gccggcgagg acgtcggaga ggtcccagag gctgtgtcca
      301 tccatccggc caactcgaag gagccggatc cgacggtaga gatgcttggc tggacaccgg
      361 ccgagggaga ggacgatgac gatggaggcc agcccgagcc gaagacgaga tcagtcgaag
      421 cacctgccgg ggatttaggc gaggacgagg tgtctgcttc cgaccttgag atggcgaggg
      481 agatcgcgag gttcatcgcg gagcgggcgt tcgagtttga tgaccacgag gcgcggtcgt
      541 gggcactcac cgatttcgat gtgtgggccg tgctggccgg ggaatcggca cagctttcat
      601 tcgatccggt cgtaaccgac aagacccgcc gtcgctgatt cattcacctt gcgggtcttg
      661 gaggcatcga agctcgtcgt cggctgtggc ttccttcgag gcgaacttcg tgtgatcgcg
      721 gaacgaaagc ggcgggaaac acattggtgt cggtcaatta tatctgttac agtcaatatg
      781 actgtggacg tcgttgactt gagaaccatc ctgtttcacg gcctgtcgga cccgactagg
      841 cagcggatag tcgagttcct gcggaccaat tcaccagccc gggttaccga tgttgttgcg
      901 gcgattggca tctcccagcc gaacgcatca actcatctgg cttgcctttg ggagtgcggg
      961 gttgctgagc gcgaacggcg gggccgggag gttcactacc ggcttggcga aagcgttgaa
     1021 gagcttctag ggactgcgga cgttctgctt gatcgtgccg gggatcggat cgagtcttgc
     1081 ccgaactacg gccgtggccg gcggcggcag gccgcgtgag ttcctgcgac tgcggcaact
     1141 cggaggccgc gaccggtctg agctggcgcg aggcagcatt tcgtttcggg ccgcttcgaa
     1201 acgcactgct cgccgggatc gctcttgttg tcggcctcgt cctggagttc accggcgcgt
     1261 cggaagtgct ggcgatcgca gcgttcctcg tagcgatccc aataggggct tggtatttca
     1321 ctcaggaggg gtgggaggaa ctcgtcgagg agcacgagat aggcgtcgag gcactgatgc
     1381 tgatcgctgc tgccggctgt ctcgttttcg gccttttcga agaagcggcg gcactggttt
     1441 ttctctactc ccttgctgaa gcgattgaag aactgacctt cgttcggacc cggtcggcga
     1501 tccgggatct gctcgatctc gccccaaagc aagcgagacg aatcgtcaac ggacgcgaag
     1561 aaatgattcc ggccgaagat ctcgtcgtcg gtgacctgtt catggtccgg cccggagagg
     1621 ctctgccgac cgacggtgtc cttagaagtg gcaactcgac gcttgacgaa gcacccgtga
     1681 ccggagagtc gatgccagtt gccaaggatc cgggacaaga ggtgttcgcg ggttcggtga
     1741 acggaaccgg ctcgatcgag atcgaggtca caaggccgta cgccgacaac accctccggc
     1801 gcatcgttca tctggtcgag gaagcgcagg gtcagaagtc gaaggcacaa cgcttcgtcg
     1861 accggttcgc gactcgatac agcccggccg tcttggtggg tgcggcgctg gtcgccgtcg
     1921 tcggcggcat cgtggacggc gactggcaag aatgggctct gcgaggcgta acggtcctgg
     1981 tggccggcgc cccttgtgcc ctcgttatgt ccgtgccggt cgcggctgcc gcagctatca
     2041 cccgggcagg gcgggaggga attctcatca agggaggcct gcaactggaa gcactcgggc
     2101 gggtcaatgc ggtcgcgttc gacaagaccg gaactctcac tcgtggcaag cccgaagtca
     2161 ccgacgtggt cgccttgaac gacgtcgccg aaagcaaggg gctacggatc gcagcgtcta
     2221 tcgaagcgcg gtctgaacac ccattggccg cggcggtggt cgcttacgcc cgcacggcag
     2281 gaatcgagcc cggtccagct gaggaattct ctgccctagt agggcacgga gcaaccgcgc
     2341 ttgtggaagg ccgaacggcc tgggtcggga gtccggcttt ggctatcgag cgcatgggat
     2401 cgggcgaact acctgacatc gtcgagaagc tgca
//
LOCUS       DAOTIP010000297         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51441_length_2432_cov_2.740850, whole genome shotgun sequence.
ACCESSION   DAOTIP010000297 DAOTIP010000000
VERSION     DAOTIP010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51441_length_2432_cov_2.740850"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="atpA"
                     /locus_tag="P5138_06335"
     CDS             complement(<1..1075)
                     /gene="atpA"
                     /locus_tag="P5138_06335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006096654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRV60230.1"
                     /translation="MQIKPDEIASILRQRIEGIDPGSADLTEVGTVLSVADGIARIHG
                     LDNCMALEMLDLPHGVTGLALNLEADNVGAVLFGDWDKIVEGDTVKRTGRLLEIPVGE
                     EFLGRMVDPLGRPLDGKGDVKSTETRPAEFKAPGVISRQPVREPLQTGIKSIDGMIPI
                     GRGQRELIIGDRQTGKTSVTIDSIINNKDTGVISVYVAIGQRMSTIVQVREVLEAAGA
                     MENTIIVAAPADEAAPIKFIAPYAGTAMAEYFLYKGGHALCIYDDLTKHAYAYRQMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAVKLSDEEGGGSLTSLPIIETQANDVSAYIPTN
                     VISITDGQIFLESDLFNSGVRPAI"
     gene            complement(1093..1626)
                     /gene="atpH"
                     /locus_tag="P5138_06340"
     CDS             complement(1093..1626)
                     /gene="atpH"
                     /locus_tag="P5138_06340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRV60231.1"
                     /translation="MEELARVYAESLFGVAREKGELDEVQAQLAQFSDALEGNRELAV
                     FFFSPSFSSQEKLDGLNRAVEGANPEVLNFLELLIEKHRMPAIFRIRRDFDDLWKKEN
                     DRIEVTVTSAVELDPAVSREVADAVEKQTGKQVDLTSRVDEGIIGGLVLQVGNMVLDS
                     SIRNNLEKLRQSVAQAA"
     gene            complement(1626..2186)
                     /gene="atpF"
                     /locus_tag="P5138_06345"
     CDS             complement(1626..2186)
                     /gene="atpF"
                     /locus_tag="P5138_06345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRV60232.1"
                     /translation="MLPLAAEAAEEEGGSFLVTPGVGLMIWTLVLFGFTFWVLKKFAF
                     PLIGEALDKRAATIRESIEAAEKQKEESDKLLADYRQRLTEAREQADEIVAKARKAAE
                     TTKSEATADGQAKREELVDAARKDIEIEKTKALDDIRKEVANLTVLATERVTRKSLDD
                     SAHKELVEDALREVDFSALAGEDRNN"
     gene            complement(2224..2427)
                     /gene="atpE"
                     /locus_tag="P5138_06350"
     CDS             complement(2224..2427)
                     /gene="atpE"
                     /locus_tag="P5138_06350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRV60233.1"
                     /translation="MALGVGAGLGSIGAGIGIGFIFGKEIESVARQPELASELQSIRW
                     LGFALTEAVAFYTFIFALIAFFL"
BASE COUNT          411 a          756 c          787 g          478 t
ORIGIN      
        1 tgatggccgg gcgcacgccc gagttgaaga ggtccgactc gaggaagatc tgaccgtcgg
       61 tgatcgagat gacgttggtc gggatgtagg ccgaaacgtc gttggcctgg gtctcaatga
      121 tcggcagcga ggtcagtgat ccgccgccct cttcgtcgct cagcttgacc gcacgctcga
      181 gcagacgcga gtgcaggtag aagacgtcgc ccgggtaggc ctcacgtccc ggaggacgac
      241 gcaagagcag cgacatctgg cggtacgcgt aagcgtgctt ggtcaagtcg tcgtagatgc
      301 agagggcgtg accgcccttg tagaggaagt actcggccat tgccgtaccg gcatagggcg
      361 cgatgaactt gatcggagcg gcttcgtcgg ccggggcggc gacgatgatc gtgttctcca
      421 tggcaccggc ggcttcaaga acctcacgga cctggacgat ggtcgacata cgctgaccga
      481 tcgccacata caccgagatg accccggtgt ccttgttgtt gatgatcgag tcgatcgtga
      541 ccgaggtctt gccggtctgg cggtcgccga tgatcagctc gcgctgtcca cgaccaatcg
      601 ggatcatgcc gtcgatcgac tttatgccgg tctggagcgg ctcgcggacg ggctgccggc
      661 tgatcacgcc gggcgccttg aactcggccg gtcgggtctc ggtcgacttg acgtcaccct
      721 tgccgtcgag cggacggccg agcgggtcga ccatgcggcc gaggaattcc tcgccgaccg
      781 ggatctcgag caggcggccg gtgcgcttga ccgtgtcgcc ttcgacgatc ttgtcccagt
      841 caccgaagag cacggcgccg acgttgtctg cctcgaggtt gagggccagc ccggtcaccc
      901 cgtggggcag gtcgagcatc tcgagggcca tgcagttgtc gagcccgtga atacgggcga
      961 taccgtcagc gaccgagagc acggtgccga cctcggtcag gtcggcgcta ccggggtcga
     1021 tgccctcgat gcgctgccgc agaatgctgg caatctcgtc tggcttgatc tgcatctcgg
     1081 tccaaatcct ccttaggccg cctgagccac gctctggcgg agtttctcca agttgttgcg
     1141 aatgctgctg tcgagaacca tgttcccgac ctgaagtacg agtccgccga tgatcccttc
     1201 atcgacgcgg ctggtcagat cgacctgctt gccggtctgc ttttcaaccg cgtcggcgac
     1261 ttcccgggac acggccggat cgagctcgac cgcactggtc acggtcactt cgatccggtc
     1321 gttttccttc ttccagaggt catcgaaatc gcgccggatc cggaaaatgg ccggcattcg
     1381 gtgcttttcg atgagcagtt cgaggaagtt gagaacttcc gggttcgccc cctcgaccgc
     1441 cctgttcaga ccgtcgagct tctcctggga tgaaaaagag gggctgaaga agaagaccgc
     1501 gagctcgcgg tttccttcga gggcatcgct gaactgggca agctgcgcct gcacttcgtc
     1561 cagctcgccc ttctccctgg ctacgccaaa gagggactcg gcatataccc gagccagttc
     1621 ttccatcagt tgttccgatc ctcgccggcc agggccgaga agtcgacctc gcgaagggcg
     1681 tcttcgacca gctccttgtg ggcggagtcg tcgagcgact tgcgggtcac ccgttcggtc
     1741 gcgagaacgg tcagattggc gacttccttg cggatgtcat cgagagcctt ggtcttctcg
     1801 atctcgatgt ccttgcgggc cgcgtcgacc agctcttcgc gcttggcctg cccgtcggca
     1861 gtcgcttccg acttggtcgt ctcggctgcc ttgcgggcct tggcgacgat ttcgtcggcc
     1921 tgctcgcgag cttcggtcag tcgctggcgg tagtcggcca gcagcttgtc cgattcttcc
     1981 ttctgcttct cggcagcttc gatgctctcg cggatggttg ccgcgcgctt gtcgagggcc
     2041 tcgccgataa gcgggaaggc gaacttcttc agcacccaga aggtgaagcc gaagagcacc
     2101 agggtccaga tcatcagacc cacgcccggc gtcaccagga agctgccgcc ctcttcctcg
     2161 gccgcttcgg ctgccagcgg cagcacggtc gcgatatgtg agagaacagg ctccacggtc
     2221 ggcctacagg aagaacgcga tcagcgcgaa gatgaaggtg tagaaggcga cggcctcggt
     2281 cagagcgaag ccgagccagc ggatcgactg gagctcggaa gcgagctcgg gctgacgggc
     2341 gaccgactcg atctccttgc cgaagatgaa gccgatgccg atgccggcgc caatcgagcc
     2401 gagtccggcg cctacgccca gggcgatcgc ct
//
LOCUS       DAOTIP010000298         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51443_length_2432_cov_2.560370, whole genome shotgun sequence.
ACCESSION   DAOTIP010000298 DAOTIP010000000
VERSION     DAOTIP010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51443_length_2432_cov_2.560370"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="P5138_06355"
     CDS             complement(<1..181)
                     /locus_tag="P5138_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRV60234.1"
                     /translation="MICLVGFMGAGKSTALVELAAHGLHSVDADSLIEERAGRPIARI
                     FEDDGGKMNLSLEDVG"
     gene            complement(178..1338)
                     /gene="aroC"
                     /locus_tag="P5138_06360"
     CDS             complement(178..1338)
                     /gene="aroC"
                     /locus_tag="P5138_06360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRV60235.1"
                     /translation="MSFRFTTAGESHGPGLVTIVEGLPAGLELDRETLDRDMARRQLG
                     HGRGGRMKIETDRVEMRAGVRHGLTMGGPVAALVANRDYKNWDERMNPWPIEGEIPPV
                     TMPRPGHADLVGMWKYKHDDLRPILERASARETAARVAAGAIARAFLTAVGVTVRSHV
                     TQIGSVKSPETDDLSIGDFDGVDEDPVRCLDPETSAAMVEEINVLRKKNESLGGTFEV
                     RVFGLVPGLGSHISWEDRLDGRLAQAICSIQSIKGVSIGQAWDVAGRPGSAAHDEIFY
                     SEERGYYRETNHAGGLEGGMTNGMPLSVRAAIKPISTMTKPLRSVDTATKEPAQAHKE
                     RTDSTVVPAAAVVGEAMICLAMVKTYREKFGGDHMDDVLAAIDAYKERIGWR"
     gene            1410..1877
                     /locus_tag="P5138_06365"
     CDS             1410..1877
                     /locus_tag="P5138_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60236.1"
                     /translation="MAARRLLIIMLVLLGISSVIAIVVPEPRRDDPGSGESVTGATGA
                     TSATGATSATGPTGDTGDSGKMVASQTGVEHRTVDLEAKQMSRVRAEAGSRMILTVKS
                     SQGSDVEIEGLGLAGFADPYAPAVFDVIMPGEPGRYVVRAPGDRPSAVIITSS"
     gene            complement(1887..>2432)
                     /locus_tag="P5138_06370"
     CDS             complement(1887..>2432)
                     /locus_tag="P5138_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRV60237.1"
                     /translation="SFRDGMVLAENTDAPGLIDALADAAAGADRRRALVLGAGGAGRA
                     VVWGLARDGYEVMLWNRTRSRAEALAAELGVEAIPSDQHAAIDPGPFEVIVNASAAGL
                     GGGDGLAELPLDPGGFHAGQTVVDMVYGETEGTLVATARQDGAATIDGLEILVRQGAR
                     SFEIWTGKSPDLGVMRRAARA"
BASE COUNT          387 a          844 c          767 g          434 t
ORIGIN      
        1 cgccgacgtc ctcgagggag aggttcatct tcccgccgtc gtcctcgaag atccgggcga
       61 tcggccgacc ggcacgttcc tcgatcaggg agtcggcgtc gacgctgtgg agcccgtggg
      121 cggcgagttc gaccagggcc gtcgactttc cggcgcccat gaacccgacc aggcagatca
      181 tcgccagccg attcgctctt tgtaagcgtc gatcgcggcc agcacgtcat ccatgtgatc
      241 gccaccgaac ttttcccggt aggtcttgac catcgccagg cagatcatcg cttcaccgac
      301 cacggcggcg gccgggacca cggtcgagtc ggttcgctcc ttgtgtgcct gggccggctc
      361 cttcgtcgcg gtgtcgaccg agcgcaacgg cttcgtcatg gtcgaaatcg gcttgatcgc
      421 ggcgcgaacc gagagtggca ttccgttggt catgccgcct tccaggccgc ccgcgtggtt
      481 cgtctcgcgg tagtagcccc gttcctccga gtagaagatc tcgtcgtggg cagcggaacc
      541 gggccggccg gcgacatccc aggcctgacc gatcgagacg cccttgatcg actggatcga
      601 gcagatcgcc tgggcgagac gcccgtcgag gcggtcctcc caggaaatgt gacttccaag
      661 ccccgggacc aggccgaaga ccctgacctc gaaggtgcca ccgaggctct cgttcttctt
      721 gcgcaggacg ttgatttcct cgaccatggc cgctgacgtt tccggatcca ggcagcgcac
      781 cggatcttcg tcaacaccgt cgaagtcccc gatcgaaagg tcatcggtct ccggagactt
      841 caccgatccg atctgggtga catggctgcg gaccgtcacc ccgaccgcgg tcaggaaggc
      901 ccgggcaatc gccccggcgg ccaccctggc ggcggtctcg cgggcactgg cgcgctcgag
      961 tatcggacgc aggtcatcgt gtttgtactt ccacatgcca accaggtctg catgtccggg
     1021 gcgcggcatg gtgaccggcg ggatctctcc ctcgatcggc cacggattca ttcgctcgtc
     1081 ccagttcttg tagtcccggt ttgcgaccag cgcggccacg ggaccaccca tggtcagtcc
     1141 gtgacggacc ccggcacgca tctcgacccg gtcggtttcg atcttcatgc gaccgccgcg
     1201 accatggccg agctgacgac gggccatgtc gcggtcgagt gtctcccggt ccagttcgag
     1261 tccggccggc agaccttcga cgatcgtcac cagcccgggc ccgtgggact cgcccgcagt
     1321 tgtaaatcgg aaactcattg agacttctag gctattgaca actgcccgcc cgacgctgtc
     1381 tcaaccgacc cggggatcga catctgcaaa tggccgcccg tcgcctgctg ataatcatgc
     1441 ttgtcctgct ggggatttcc tcggtgatcg cgatcgtcgt tcccgaaccc cggcgggacg
     1501 atccgggctc cggcgagagc gtgaccggcg ccactggagc aacgtcggcc actggtgcga
     1561 cttcggcgac agggccgacc ggagacaccg gcgattccgg caagatggtc gcttcccaaa
     1621 ccggagttga acacaggact gtggacctcg aggcgaagca gatgtcccgg gtccgggccg
     1681 aagccggcag ccggatgatc ctgaccgtga aatcgagcca gggatccgac gtcgagatcg
     1741 aaggtttggg cctggccggc ttcgcggatc cctatgctcc ggcggtcttc gacgtgatca
     1801 tgcccgggga gccaggacgc tacgtggtac gcgctccggg agacaggccg agcgcagtga
     1861 tcatcaccag ttcctgagcc ggtctctcag gcgcgggcgg cccttcgcat tactcccagg
     1921 tccggactct ttccggtcca gatttcgaag gatcgggctc cctgccggac caggatctcg
     1981 agcccgtcaa tcgtcgcggc accatcctga cgggcggttg caaccagggt gccttcggtt
     2041 tctccgtaaa ccatgtcgac cacggtctgt ccggcgtgaa atccgccggg atccagcggc
     2101 agctccgcca gcccgtcgcc gcccccgagg ccggccgccg acgcgttgac gatcacctcg
     2161 aacggtccgg ggtcaattgc cgcatgctgg tcggagggaa tcgcctcgac accgagttct
     2221 gcggccagag cctccgccct ggaccgggtc cggttccaga gcatcacctc gtatccgtcc
     2281 cgggccaggc cccagaccac ggcccgcccg gctcctcccg ctccgaggac gagcgccctt
     2341 ctccgatcgg cgccggccgc ggcatccgcg agtgcgtcga tcaggccggg agcgtcggtg
     2401 ttctcggcca gcaccatccc gtcccggaag ct
//
LOCUS       DAOTIP010000299         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51472_length_2431_cov_2.837542, whole genome shotgun sequence.
ACCESSION   DAOTIP010000299 DAOTIP010000000
VERSION     DAOTIP010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51472_length_2431_cov_2.837542"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="P5138_06375"
     CDS             complement(<1..936)
                     /locus_tag="P5138_06375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRV60238.1"
                     /translation="MSNDRIKLLPLGGLGEIGKNMMVIEYRGKIVVVDTGLMFPTPDM
                     LGIDLVLPDFEYLRERANDIEAIVLTHGHEDHVGALPYVLREIGFPKVIYGGMLTVGM
                     VRSKLDEHKLGSAPLTELPAGQKVDAGPFEIELVHLSHSIPDMRGVIVGTDIGKIFMT
                     GDYKFDQTPVDGRPADVARLAALGDEGLLLLCGDSTNADRPGVSPSESSVGPALYETF
                     SRCKGRIVVTSFASNIHRVQQVIDAAARLDRKVALVGRSMRKNFNIASNLGVANAPDG
                     LLIQPREIEDYPDERVVVISTGSQGEPFSALRRMAN"
     gene            complement(933..1802)
                     /gene="dapA"
                     /locus_tag="P5138_06380"
     CDS             complement(933..1802)
                     /gene="dapA"
                     /locus_tag="P5138_06380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRV60239.1"
                     /translation="MSEFRGVITALATAFRPDGELDVQSTRKLAAYLVENGSHGLVVA
                     GSTGESSTLEDDEKIELTRAVVDEVGPDVTVIVGSGSNDTRHSVELTKMAAEAGADAA
                     LIVTPYYVRPNRAGIMGHFEAIAGGSPGFPLVAYNIPSRTGINMPPDFLAELAGIDEV
                     KAVKQANDDDLGPIEGLDVLAGNDGTFLPTLKFGGAGGILVASHLDNGRMRTIWDLWQ
                     EGRHDEAEALDNQLQPLYEAMGVVVNPIPVKAALAARGLLPPTMRLPLVEPTDQERDA
                     VEELLAGAGVPAA"
     gene            complement(1840..>2431)
                     /locus_tag="P5138_06385"
     CDS             complement(1840..>2431)
                     /locus_tag="P5138_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRV60240.1"
                     /translation="EDLGHEVTEFDLPAAGEPEALYKTFITRWAAGMSQTSDTLSLII
                     GRPLTRDDVEPLTWALIEKGRTEGGAAYLSAVAQHQLIARLIAGVMIDGQFDLIMTPT
                     LATVPPKLGHFDQNGPDPMDAMVKAREIATFTGIFNATGQPAISLPLEMSEEGLPIGI
                     QFVAPMWGEDVLIQLAGELERAHPWIDRRAPGVLDA"
BASE COUNT          399 a          793 c          804 g          435 t
ORIGIN      
        1 gttggccatc cgcctcagcg ccgagaaggg ctcgccctgg gagccggtcg agatcaccac
       61 gacgcgctcg tccggatagt cctcgatctc gcgcggctgg atcagcagcc cgtcgggggc
      121 gttcgccacc ccgaggttcg aggcaatgtt gaagttcttc cgcatcgaac ggccgaccag
      181 ggcgaccttg cggtccagcc ttgcggcggc atcgatcacc tgctggacgc ggtggatgtt
      241 ggaggcgaag gaggtgacca cgatcctgcc cttgcagcgg gagaaggtct cgtaaagggc
      301 cgggccgacg ctggattcgg agggcgagac acccggccgg tcggcgttgg tcgaatcacc
      361 gcagagcagc agcagcccct cgtcacccag cgcggcgagg cgggccacgt cggcgggccg
      421 gccgtcgacc ggggtctggt caaacttgta gtcgccggtc atgaagatct tgccgatgtc
      481 ggtaccgacg atcaccccgc gcatgtcggg tatcgaatgc gaaaggtgga ccagttcgat
      541 ctcgaagggc ccggcatcca ccttctgccc ggccggcagt tcggtcagcg gggcgctgcc
      601 gagtttgtgc tcgtccagct tggagcggac cattccgacc gtgagcatgc cgccgtagat
      661 gaccttcggg aaaccgatct cccgcagcac gtagggcagc gcccccacgt ggtcctcatg
      721 gccgtgggtg agcacgatcg cctcgatgtc attggcccgt tcgcgcaggt actcgaaatc
      781 gggcaggacc aggtcgatcc ccagcatgtc cggggtcggg aacatcaggc cggtgtcgac
      841 caccacgatc ttgccgcggt actcgatcac catcatgttc ttgcctatct cccccagtcc
      901 gccgagggga agaagcttga tccggtcgtt gctcatgcgg caggcacacc ggccccggcg
      961 aggagttcct ccacggcgtc acgttcctgg tcggtcggct cgaccagcgg cagccgcatg
     1021 gtcggtggca gaagtccacg ggcggcaagg gcggccttga ccgggatcgg attgacgacc
     1081 acgcccatcg cctcgtaaag gggctggagc tggttgtcca gcgcttcggc ctcgtcgtgg
     1141 cggccttcct gccagagatc ccagatcgtc ctcatgcggc cgttgtccag gtgggaagcc
     1201 acgaggatgc ctcctgcccc tccgaacttc agggtcggga ggaaagtgcc gtcgttgccg
     1261 gcgaggacat ccagcccttc gatcggtccg agatcgtcgt cgttggcctg cttgaccgcc
     1321 ttgacctcgt cgatcccggc cagttccgcg aggaaatcgg gtggcatgtt gatgccggtg
     1381 cgggaaggga tgttgtaggc gaccagcggg aagcccgggc tgcccccggc gattgcctcg
     1441 aagtggccca tgattccggc ccggttcggc cgcacgtagt agggagtgac gatcagggcc
     1501 gcgtcggcgc cggcctcggc cgccatcttg gtcagttcca ccgagtgacg ggtgtcgttg
     1561 gagccggagc cgacgatcac ggtcacgtcg ggcccgacct cgtccactac cgccctggtc
     1621 agctcgatct tctcgtcgtc ttccaaagtc gaagactcac cggttgatcc cgcgaccaca
     1681 agaccatggc ttccgttctc gaccagatag gcagcgagct ttcgcgtcga ttggacgtcg
     1741 agttctccgt cgggccggaa agcggtggcc aaagccgtga ttacacctcg aaattcactc
     1801 acgacaggca atctatccca caaccgactg ccgttcagtt caggcgtcga gcacgcccgg
     1861 cgcccgccgg tcgatccacg gatgggcacg ctcgagttca ccggccagct ggatcaaaac
     1921 gtcctcaccc cacatcggag cgacgaactg gataccgatc ggcagacctt cctcgctcat
     1981 ttccagcggc agcgaaatcg ccggctggcc ggtcgcgttg aagatcccgg tgaaggtggc
     2041 gatctcccgc gccttcacca tcgcgtccat cgggtcgggc ccgttctggt cgaaatggcc
     2101 gagcttcggg ggtacggtgg cgagagtcgg ggtcatgatc aggtcgaact gtccgtcgat
     2161 catcacgccg gcgatcaggc gggcgatcag ctgatgctgg gcaacagcgg agaggtacgc
     2221 ggccccgcct tcggtcctgc ccttctcgat cagggcccag gtcaggggct cgacatcgtc
     2281 ccgggtcagg ggacgcccga tgatcaggct gagcgtgtcc gaggtctggc tcatccctgc
     2341 cgcccagcgg gtgatgaagg tcttgtagag cgcctcgggc tcgccggccg ccggcaggtc
     2401 gaactcggtc acctcgtggc cgagatcctc c
//
LOCUS       DAOTIP010000300         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51673_length_2424_cov_3.311946, whole genome shotgun sequence.
ACCESSION   DAOTIP010000300 DAOTIP010000000
VERSION     DAOTIP010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51673_length_2424_cov_3.311946"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="P5138_06390"
     CDS             <1..508
                     /locus_tag="P5138_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60241.1"
                     /translation="APGCLDAADAGLMVDARGIARPVEGHCDSGSWQHEGPVGPTGPT
                     GPTSPTGPTGPTSPTGPTGPTGPSGNGARMMLGKPKLKPKKGTAVVPVKVSGPGVIRL
                     RGNNSVQGQTRKPGKAKTVRIPVRLKAPARQKLAGRGRLKVILRFRFKPTSADAVTKA
                     RKLTLRMK"
     gene            complement(563..>2424)
                     /gene="acs"
                     /locus_tag="P5138_06395"
     CDS             complement(563..>2424)
                     /gene="acs"
                     /locus_tag="P5138_06395"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRV60242.1"
                     /translation="WWAGWAEKLDWIEPWETVLDWSNPPHATWFAGGKLNVSANCLDR
                     HVEAGRADEVAYHWEAEDAGPDDEPVTGTGSHAGGRISVTYGWLHDQTQRLANVLRDR
                     GVKKGDVVGIFMPMVPEAAVAMLACTRIGAVHNVVFGGFSAESLRERMEFSDAKALIT
                     ADATLRRGNPVPMKSAIDDALDELPSMELVVVFDRCGTDAPLKEGRDIDWASALEAAD
                     PVCEPEPMDAEDPLFLLYTSGSTAKPKGIVHTTGGYLTQATATHRMIFDLKDDDVYWC
                     AADIGWVTGHSYIVYAPLANGATSIMFEGAPDFPTKDRWWEIVERYRATILYTAPTVI
                     RSFMKWGDELPAAHDLSSLRLLGTVGEPINPKAWLWYHEHIGAERCPIVDTWWQTETG
                     AAMISPLPAITTTKPGSATRPLPGFRAAIYDNNGNEIEEGGGTLVLTRPWPAMARTLF
                     KEPERYINAYWDRYGRETYLVGDAARRDEDGYFWIVGRIDDVINVSGHRLSTMEIESS
                     LVSHPHVAEAAVIGVPDDFTGQAIIAFAIPNDEAPPGHEFTDELREHVAVRIGKLARP
                     KAIYFADDLPKTRSGKIMRRLLRDIADGKELGDVSTLRDPGVVEELQAQVAEA"
BASE COUNT          384 a          799 c          824 g          417 t
ORIGIN      
        1 agcccccgga tgcctcgacg ccgctgacgc gggcctgatg gtcgacgccc ggggcatcgc
       61 ccgtccggtc gaaggtcact gcgattcggg ctcctggcag cacgagggac cggtcggccc
      121 gaccggaccc accgggccga ccagtccgac cgggcccacc gggccgacca gtccgaccgg
      181 accgacgggc ccgaccggac cttccggcaa cggggcccgc atgatgctcg gcaagccgaa
      241 gctgaagccg aagaagggga ctgccgtggt gccggtcaag gtgtccgggc ccggagtcat
      301 ccggctcagg ggaaacaact cggttcaggg ccagacccgc aagcccggca aggcgaagac
      361 ggtccggatc ccggtcaggc tgaaggcccc agcacgtcag aagctggcgg gcaggggtcg
      421 tctcaaggtc atcctcaggt tccgcttcaa gccgaccagc gcagacgcgg tgaccaaagc
      481 caggaagctg accctgcgga tgaaatagcg gccggcccgg tgtcgtgcgg caatgactgc
      541 cgtgcggcac cgggcgcggc tgtcaggctt cggcgacctg cgcctggagt tcctcgacca
      601 cgccgggatc acggagagtg ctgacgtcgc ccagctcctt gccgtcggcg atgtcgcgga
      661 gcagacggcg catgatcttg ccggagcggg tcttggggag atcgtcggcg aagtagatcg
      721 ccttcgggcg cgcgagcttc ccgatgcgga cggccacgtg ctcgcgcagc tcgtcggtga
      781 actcgtggcc ggggggcgct tcgtcattgg gtatcgcgaa ggcgatgatg gcctgtccgg
      841 tgaagtcgtc cggcaccccg atcaccgcgg cttcggcgac atgggggtgg gagaccagtg
      901 aggattcgat ttccatcgtg gaaagccggt gccccgaaac gttgatcacg tcatcgatgc
      961 gtccgacgat ccagaagtag ccgtcctcgt cacgccgggc ggcgtccccg accagatagg
     1021 tctctctgcc gtatcggtcc cagtaggcgt tgatgtaacg ctccggctcc ttgaacagcg
     1081 tccgggccat cgccggccag ggccgggtga ggaccagggt tccgccaccc tcctcgatct
     1141 cgtttccgtt gttgtcatag atcgctgccc tgaagcccgg tagcggccgg gtggctgaac
     1201 cgggcttggt cgtcgttatg gccggcagcg gggagatcat cgcggcgccg gtttcggtct
     1261 gccaccaggt gtcgactatc ggacagcgtt cagcgccgat gtgttcgtgg taccagagcc
     1321 aggcctttgg attgatcggc tctccgaccg ttcccagcag cctgagcgag ctcaggtcat
     1381 gtgcggccgg aagctcgtcg ccccatttca tgaacgaccg gatcacggtc ggggccgtgt
     1441 agaggatcgt ggcccgatac cgctcgacga tctcccacca gcgatccttg gtcgggaaat
     1501 ccggagcgcc ttcgaacatg atcgaggtgg cgccgttggc cagcggtgcg tagacgatgt
     1561 aggaatgccc ggtcacccat ccgatgtcgg ccgcgcacca gtacacatcg tcgtccttca
     1621 ggtcgaagat catgcgatgg gtggcggtgg cctgggtcag gtagccgccg gtcgtgtgca
     1681 cgattccctt gggcttggcg gtcgaccctg aggtgtagag caggaaaagc gggtcctcgg
     1741 cgtccatcgg ctccggttca caaaccgggt cggccgcttc cagcgcgctg gcccagtcga
     1801 tgtcccgtcc ttccttcagc ggggcgtcgg ttccgcaacg atcgaacacg accaccagtt
     1861 ccatggacgg gagctcgtcc agggcgtcgt cgatcgccga cttcatcggc acgggattgc
     1921 cgcggcgcag ggtggcgtcg gcggtgatca gggccttggc gtcggagaac tccatccgct
     1981 ccctgagcga ctcggccgag aatccgccga agacgacgtt gtgtaccgcc ccgatcctgg
     2041 tgcaggccag catcgcgacg gcggcttcgg gaaccatcgg catgaagatc ccgaccacat
     2101 ctcccttctt cacccctcgg tccctgagga cattcgccag acgctgggtc tgatcgtgga
     2161 gccagccgta ggtgaccgag atgcgacccc cggcgtggct gccggttccc gtcacgggct
     2221 cgtcgtccgg acccgcgtcc tcggcttccc agtggtaggc gacctcgtct gcccgccctg
     2281 cttcgacgtg gcggtccagg cagttggcgc tgacgttcag cttgccgccc gcgaaccagg
     2341 tggcatgggg cggattcgac cagtcgagca cggtctccca gggttcgatc cagtccagct
     2401 tctcggccca gccggcccac cagc
//
LOCUS       DAOTIP010000301         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51702_length_2423_cov_3.678209, whole genome shotgun sequence.
ACCESSION   DAOTIP010000301 DAOTIP010000000
VERSION     DAOTIP010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51702_length_2423_cov_3.678209"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5138_06400"
     CDS             <1..230
                     /locus_tag="P5138_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV60243.1"
                     /translation="FGHHHDKAEEVYFVVSGSGRAKLDDEIVELKEGSLLRVGPEVTR
                     CFEAGPQGMEYLAMGQHFSGDGGVEPGWWSD"
     gene            complement(311..712)
                     /locus_tag="P5138_06405"
     CDS             complement(311..712)
                     /locus_tag="P5138_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60244.1"
                     /translation="MATLEHEVIGPKPVNGNRKSIASLLIGALGLSSSLVLVIGLWQG
                     STDDWVENYWAGLAFIGLFLGAIAVFLGSIGWIDIRRGATDRGKFASTVGIVLGVVGA
                     LLVLAVFAYVVWILIDIIQWAGTNDATGGWD"
     gene            complement(745..1350)
                     /locus_tag="P5138_06410"
     CDS             complement(745..1350)
                     /locus_tag="P5138_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRV60245.1"
                     /translation="MPATDNEIKEKYLERAIRELNQLSRDLQSCDACLATRVMPVLGS
                     GHPQADIMLVKFSPTTAEVEEGVAYYGRAGNALMKSFKRLSIDPMVIYGTVCAKCPVE
                     DPAQADPECVARLADEFNIVQPRVVVVMGEPALETINSLALPLARPLDAREGEIQAFT
                     PACDALFTPDIDGALDEETAKRRFWRAFRTLGRWHDDLPPY"
     gene            complement(1419..>2423)
                     /locus_tag="P5138_06415"
     CDS             complement(1419..>2423)
                     /locus_tag="P5138_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV60246.1"
                     /translation="DGQRGLLFPAGDPVTLAGQIQRLGEDGRLRERLRSACGKFEIET
                     WPEVADRFESTYERLVARRHDPAGNEALRKKLRDRPLIDVDLHMHTDHSSDCATPVKV
                     LIETARDRGFGAIAITDHNEVSGAREAAKVAEEMDDFKIIVAEEVKTGEQGEVIGLFL
                     KEKIPKGLTMDETIAEIKRQGGLVYVPHPFDRLHSVPDYENLLDMVEDIDLMEVFNPR
                     VAISSFNEEAERFARKYNIIPAAGSDSHVAQGLGAVRNRLHDFDGPEEFLEAMREAEI
                     TRRHKNLVYVQALKFIQTTGRPKAAKREVEAPRAVKGGRRKARGPKQGRGSKRAAGKS
                     "
BASE COUNT          392 a          785 c          800 g          446 t
ORIGIN      
        1 ctttcggcca ccaccacgac aaggccgagg aggtctactt tgtggtctcc ggttcagggc
       61 gggccaagct cgacgacgag atcgtcgagc tgaaggaggg cagcctgctc cgggtcgggc
      121 ccgaggtcac gagatgcttc gaagccggcc cgcaaggaat ggaatacctg gcgatgggcc
      181 agcatttctc cggcgacggc ggagtcgaac ccggctggtg gagcgactga gctccaaccc
      241 gggcgctagt ccaccgtcgc cccgccctcg ccaaccagag ctacatttta agcggctaga
      301 gacccgcgaa tcagtcccag ccgcccgtgg catcgttggt tccggcccat tgaatgatgt
      361 cgatcaagat ccacacgacg taagcgaaca ccgcgagcac aagaagggcg cccaccactc
      421 ccagaactat tccgactgtg gaagcgaact tgcctcggtc ggtggctccc cgccgaatgt
      481 cgatccagcc gatcgagccg agaaacacgg caattgcgcc gagaaagaga ccgataaagg
      541 caaggccggc ccagtagttt tcgacccagt catcggtgct gccctgccag aggccgatca
      601 cgagcaccaa cgaacttgac aggcccagcg ccccgataag cagcgaagct attgacttcc
      661 gattgccatt gactggcttc ggcccgatca cttcatgttc aagggtcgcc atgccggaat
      721 cctaagcgcc ggcgggcatc ctggctagta gggcggaagg tcgtcgtgcc agcggccgag
      781 ggtgcggaag gctcgccaga agcggcgctt ggcggtctcc tcgtcgagcg cgccgtcgat
      841 gtccggggtg aagagggcgt cgcaggcggg ggtgaaggcc tggatctctc cttcgcgggc
      901 gtcgagggga cgggccagcg gcagggcgag gctgttgatc gtctccaggg cgggctcgcc
      961 catcaccacg acgacccggg gctggacgat gttgaactcg tcggccaggc gggcgacgca
     1021 ttcggggtcg gcctgggccg ggtcctcgac cgggcacttg gcgcagacgg tcccgtagat
     1081 caccatcggg tcgatgctca gccgcttgaa ggacttcatc agcgcgttgc cggcgcgacc
     1141 gtagtaggcg acgccctcct cgacctcggc ggtggtcggt gagaacttga ccagcatgat
     1201 gtccgcctgc gggtggcccg agccgagcac cggcatcacc cgggtcgcca ggcaggcgtc
     1261 gcagctctgc agatcacggc tgagctggtt cagctcccgg atcgcacgtt caaggtattt
     1321 ctctttgatc tcgttatcgg tcgcaggcat cgcttgagct tcttttgacg cttccggcgg
     1381 cgctcctgcc ggatcccggc tgtcaccggg cggatgcatc agctcttgcc tgctgcacgt
     1441 tttgacccac gaccctgttt cgggccacgg gccttgcgcc ggccaccctt gaccgcccgt
     1501 ggggcttcaa cttcgcgttt cgcggccttg ggacggccgg tggtctggat gaacttcagc
     1561 gcctggacgt agacgaggtt cttgtggcga cgggtgatct ccgcctctcg catcgcctcg
     1621 aggaactcct ccggcccgtc gaagtcatgc agacggttgc gcaccgcccc gaggccctgg
     1681 gcgacgtggc tgtcggaccc cgcggcgggg atgatgttgt acttgcgggc gaaccgctcg
     1741 gcctcctcgt tgaacgagga gatcgcgacc cgggggttga agacttccat caggtcgatg
     1801 tcctcgacca tgtcgagcag attctcgtag tcgggaaccg agtggagccg gtcgaaggga
     1861 tgtggcacgt agaccagccc gccctgccgc ttgatctcgg cgatcgtctc gtccatggtc
     1921 agacccttgg ggatcttctc tttgaggaag agcccgatca cctcgccctg ctcgccggtc
     1981 ttcacttcct cggcgacgat gatcttgaag tcgtccatct cttcggcgac cttcgcggcc
     2041 tccctggcgc cagagacctc gttgtggtcg gtgatcgcga tcgcgccgaa gccgcggtcg
     2101 cgggccgtct cgatcagcac cttgaccggt gtcgcgcagt cgctcgaatg gtcggtgtgc
     2161 atgtgcaggt cgacgtcgat cagcggccgg tcccggagct tcttcctgag tgcctcgttt
     2221 ccggccggat cgtgccggcg ggcgaccagt cgctcgtagg tcgattcaaa gcggtcggcc
     2281 acctcgggcc aggtctcgat ctcgaacttc ccgcaggcgg accggagtcg ttcgcggagg
     2341 cgtccgtcct caccgagccg ctggatctgg ccggccaggg tcaccggatc gccggccggg
     2401 aagagcagcc cgcgctggcc gtc
//
LOCUS       DAOTIP010000302         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51790_length_2420_cov_2.628330, whole genome shotgun sequence.
ACCESSION   DAOTIP010000302 DAOTIP010000000
VERSION     DAOTIP010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51790_length_2420_cov_2.628330"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="P5138_06420"
     CDS             <1..1358
                     /locus_tag="P5138_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60247.1"
                     /translation="WDNYVGRLVETFPDEEEGLRAVTDVMRNAMEELRSVGIPDSNTD
                     LQEYLEKAPTVVSWGMRPINELFDENFLSERSRAVILGNAGDYATPPSRTPVIMHAGV
                     IDHYLQEGAFYVKGGGQEMAGHLVDLIHTYGGRVRTHAEVERILTEETESGAHRVSGV
                     ELAGGEVVNTGNVIATGDIKHLFLELLDEEAVPEEIRNTVEGYRMATPLFTVYLGLDF
                     DITERMPATNYWLHSRYDQEAYYEPVAAGEFDTDPPVYISSASAKDPGHEGHAPPGCS
                     TIELMTWATPDPAAWGVDEGEDAPKYSRQAGYLEAKQKVTDQLIDRAEKQLGELRSHI
                     LWEEAATPMTQTRYTLSSGGSSYGIEPATDQFGPLRLEYRTPVQGLILGGVSSRRGHG
                     ILGAMAGGVESASAVLGRSLRTEIAEGAVFGDPSSLTSGGEGWDALEACRKLQEKALR
                     A"
     gene            complement(1386..>2420)
                     /locus_tag="P5138_06425"
     CDS             complement(1386..>2420)
                     /locus_tag="P5138_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60248.1"
                     /translation="VETLNFVLQLQGMAAGISRSEPWKDPNADLYDGQTFETFKLANT
                     TGDGARFLTDLATNAVWACEPRDISLLHLLFYISSAGNETTPGSFIRLTSTTGGAQES
                     RFVGGSQQLSIRMAKKLGARVVLNAPVRQIRQEKGLCHVTSDKGTWRAKRVIVAVPPA
                     VSAFIDYDPILPSDRAQLIQRFPQGNAIKVQAVYDRPFWRDEGLSGYVNSDEGPVKLM
                     YDNSPHDGSAGVLLGFIEGQEARLAGRMSPAARKAAVLANFKKTIGNAAGSPKAYVEK
                     NWAQEEWTRGCYGGFLPPGVLTGFGPALRKPHGRIHWAGTEHAEIWNGYMDGALRSGE
                     DAAKAALSKL"
BASE COUNT          408 a          797 c          817 g          398 t
ORIGIN      
        1 gctgggacaa ctacgtcggc cgcctcgtcg agaccttccc cgacgaggaa gagggcctcc
       61 gggcggtgac cgacgtgatg cggaacgcga tggaggagct ccggtcggtt ggcatccccg
      121 acagcaatac cgatcttcag gagtacctcg agaaggcccc gaccgtggtc agctggggca
      181 tgcgcccgat aaacgaactc tttgacgaga atttcctcag cgagagatca cgggcggtga
      241 ttctcggcaa tgcgggtgac tacgcgaccc ctccatcccg gactccggtg atcatgcatg
      301 cgggtgttat cgaccactac ctgcaggagg gggccttcta cgtcaagggg ggtggccagg
      361 agatggccgg ccacctggtt gacctgatcc acacctacgg cggccgggtg cggacccacg
      421 ccgaggtcga gcgcatcctt accgaagaga ctgaatcggg agcccaccgg gtctctggag
      481 tcgagctggc cggcggcgag gtcgtaaaca ccgggaacgt gatcgcgacc ggggacatca
      541 agcacctctt cctcgaactg ctcgatgagg aggcggtgcc cgaagagatc cgcaacacgg
      601 tcgaggggta tcgcatggca accccgctct tcacggtcta tctcgggctc gacttcgaca
      661 tcaccgagcg catgccggcc accaactact ggctgcattc ccgctacgac caggaggcct
      721 attacgaacc ggtggcggcc ggcgagttcg ataccgaccc gcccgtctac atctcttcag
      781 ccagcgccaa ggacccgggt cacgagggcc atgccccgcc cggctgctcg acgatcgagt
      841 tgatgacctg ggccacgccc gaccccgccg cctggggagt cgacgagggc gaggacgccc
      901 cgaagtacag ccgccaggcc ggctacctgg aagcgaagca gaaggtcacc gaccagctga
      961 tcgaccgggc cgagaaacag ctcggcgagc tccgctctca catcctctgg gaggaagcgg
     1021 cgaccccgat gacccagacc aggtacaccc tttcgtccgg cgggtcctcc tacggcatcg
     1081 agccggcgac cgatcagttc gggccgctcc ggctcgaata ccggactccg gtacaaggcc
     1141 tgattctcgg cggagtgtcg agccgccggg gccacgggat cctgggggcg atggcaggcg
     1201 gggtcgagtc tgcctccgcc gttctcggcc gcagcctccg gaccgagatc gcggaaggtg
     1261 cagtcttcgg cgatccctcg agcctcacct cgggcggcga gggctgggac gccctcgaag
     1321 cctgccgcaa gctgcaggaa aaggccctgc gcgcctgatc gcgagcctcc gcttcgggtc
     1381 cgggttcaaa gctttgagag ggctgccttg gcggcgtcct cgcccgagcg gagggcgccg
     1441 tccatgtagc cgttccagat ctccgcgtgc tcggtgccgg cccaatggat ccgcccgtgg
     1501 ggtttgcgaa gggccggacc gaacccggtc aggacgccgg gcggcaggaa cccgccgtaa
     1561 cagccgcggg tccattcctc ctgtgcccag ttcttctcca cataggcctt gggtgatccg
     1621 gccgcgttgc cgatcgtctt cttgaagttc gccaggacgg ccgcctttct ggcggcgggg
     1681 ctcatgcgtc cggccagccg cgcttcctgg ccctcgatga agccgagcag cactccggcc
     1741 gagccgtcgt gtggcgagtt gtcatacatc agcttgaccg gcccctcgtc cgagttgacg
     1801 tatccggaaa ggccttcgtc gcgccagaac gggcggtcgt agacggcctg gaccttgatc
     1861 gcgttgccct gcgggaaccg ctggatcagc tgtgcacggt cggaggggag gatcgggtcg
     1921 taatcgatga aggcgctcac cgccggcggc accgccacga tcacccgctt cgcccgccag
     1981 gtccccttgt cactggtgac gtgacagagg cccttctcct gccggatctg ccggaccggc
     2041 gcgttgagga ccacgcgggc cccgagcttc ttcgccatcc ggatcgaaag ctgctgcgag
     2101 ccgccgacga aacgtgattc ctgggcaccg ccggtggtcg aggtcaggcg aatgaagctg
     2161 ccgggggtcg tctcgttgcc ggccgaggag atgtagaaga gcaggtggag cagggagatg
     2221 tcccgcggtt cgcaggccca gaccgcgttg gtcgcgaggt cggtcaggaa acgggctccg
     2281 tcaccggtcg tgttggccag cttgaaggtc tcgaaggtct ggccgtcgta gaggtccgcg
     2341 ttcgggtcct tccacggttc ggaccgggag atgccggccg ccatgccctg cagctggagc
     2401 acgaagttca gggtttcgac
//
LOCUS       DAOTIP010000303         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51960_length_2414_cov_3.181857, whole genome shotgun sequence.
ACCESSION   DAOTIP010000303 DAOTIP010000000
VERSION     DAOTIP010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51960_length_2414_cov_3.181857"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="P5138_06430"
     CDS             <1..911
                     /locus_tag="P5138_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRV60249.1"
                     /translation="QFPTQKAFNDFLKQSGFTTEEVEFRVKLQVLSRKIQEQITKSVT
                     DVPDSEIENFYEASKSSFTTPETRDIRLIVTDKKANAEKALNELGDDPSDAEFAKVAK
                     QLSVHSSKTEGGKTVATEGAFPDPAGAAIMSADAGTIEGPIEAGKQFYVFRVTKITPE
                     ETKSLDDVRDQIKQQLLPTLQQQAMTDFVSNYNSKWTSRTFCKSEYTVSRCDNYESDG
                     RLDSADPKCYKAGAGKNPDLACPAPIELPKPMAPGGNASAGALLGQTQNLPQRPIQPA
                     DTKAADTGTGAGGSVDLSQLGAAAGQ"
     gene            940..1590
                     /locus_tag="P5138_06435"
     CDS             940..1590
                     /locus_tag="P5138_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRV60250.1"
                     /translation="MEPGELAGALERLDRVARELREKCPWDKEQDARSIVPHTVEEAY
                     ELAEAAREEDDAAMRDELGDVLFQVVFLSLLLEERGEGDLAEIAGQLTDKLIRRHPHV
                     YPPGESEATGGIETADDVRRQWDEIKRDVEGRKSEKDWRKPALPALIYANKVQWKLDP
                     EERPANAGAANPDRDEAEAEVGRLLWEAVAEARRRGVDPELALRAEAERHAEAGRK"
     gene            1604..>2414
                     /gene="eno"
                     /locus_tag="P5138_06440"
     CDS             1604..>2414
                     /gene="eno"
                     /locus_tag="P5138_06440"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936695.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRV60251.1"
                     /translation="MSTIEAVHARQILDSRGNPTIEVEVSLENEFTGRAAVPSGASTG
                     EFEAVELRDGGDPYLGKGVTQAVANVNEVIRPELIGFDVTDQAGLDRLMIELDGTENK
                     GRLGANAILGVSLAAAHASAAMEKRPLYSYLGDLYGESDPSLLPVPMMNVLNGGAHAD
                     NSVDFQEFMIMPAGTPSFSECLRVGTEVFHSLKKILAGHGLATAVGDEGGFAPDLSSN
                     EAALEMLIEGIEAAGYKPGEEVFIALDPATSEIYSDGVYRLEHEDRDLSASE"
BASE COUNT          482 a          796 c          787 g          349 t
ORIGIN      
        1 accagttccc cacccagaag gccttcaacg acttcctcaa gcagagcggc ttcacgacgg
       61 aagaagtcga gttcagggtc aagctgcagg tgctgagccg caagatccag gagcagatca
      121 cgaagtcggt caccgacgtg cccgattcgg agattgagaa tttctacgag gcgtcgaagt
      181 ccagcttcac cacgcccgag acccgcgaca tccgcctgat cgtcaccgac aagaaggcca
      241 acgcggagaa ggctttgaac gaacttggcg acgatccttc cgacgcggag ttcgcgaagg
      301 tggccaagca gctttcggtc cactcgagca agaccgaggg tggcaagacc gtagccaccg
      361 agggtgcctt ccccgatccg gccggcgccg ccatcatgag cgccgatgcc gggaccatcg
      421 agggcccgat cgaggccggc aagcagttct acgtcttccg ggtcaccaag atcacgccgg
      481 aggaaaccaa gtccctcgat gacgtgcgcg atcagatcaa gcagcagttg ctgcccaccc
      541 tgcagcagca ggcgatgacc gacttcgtct cgaactacaa ctcgaagtgg acttcccgga
      601 ccttctgcaa gtccgagtac accgtttcgc gttgcgacaa ctacgagagc gacggccgtc
      661 tcgactcggc cgaccccaag tgctacaagg ccggcgccgg caagaatccg gatctggcct
      721 gcccggcccc gatcgagctg ccgaagccga tggctccggg cggcaacgct tcggcgggtg
      781 ctctgctcgg ccagacccag aatctgccgc agcgcccgat ccagcccgcg gacaccaagg
      841 ccgcggacac cggcactggt gccggggggt cggtggacct gagccagctc ggcgcggcgg
      901 caggacagta agcacagggc aacggactgg cgggtagccg tggaacccgg cgaactcgcc
      961 ggcgccctgg agcgactcga ccgggtcgcg cgggaactcc gcgagaaatg cccgtgggac
     1021 aaagagcagg atgcccgctc gatagtgcct cacacggtcg aggaggccta tgaactggcc
     1081 gaggcggccc gtgaggagga cgacgccgcg atgcgcgacg aactcggcga cgtcctcttc
     1141 caggtggtgt ttctttccct gcttctggag gagaggggcg agggcgacct ggcggagatt
     1201 gccggtcaac tcaccgacaa gctgattcgt cgtcacccgc atgtctatcc gcccggggaa
     1261 agcgaggcga ccggaggcat cgagaccgct gacgacgtgc ggcgccaatg ggacgagatc
     1321 aagcgggacg tggaaggacg caagtcggag aaggactggc gcaagcccgc cctgccggcc
     1381 ctgatctacg cgaacaaggt ccagtggaag ctcgacccgg aagagcgtcc ggccaacgcc
     1441 ggggcggcca atccggaccg cgacgaggct gaagcggaag tcggccggct gttgtgggaa
     1501 gcggtagcgg aggcccgtcg ccgcggggtc gaccccgaac tggccctccg ggccgaggcc
     1561 gagcgtcacg cggaggccgg gcgaaagtag actggacggc caaatgagca ccatcgaagc
     1621 cgtccacgca cgccagatcc tcgactcccg gggcaacccc acgatcgagg tcgaggtctc
     1681 cctcgaaaac gaattcaccg gtcgcgccgc agtcccctcc ggtgcttcca ccggcgagtt
     1741 cgaggcagtc gaacttcgtg acgggggaga tccctatctc ggcaagggag taacccaggc
     1801 ggtcgccaac gtcaacgagg tcatcaggcc cgaactgatc gggttcgacg tcaccgacca
     1861 ggccggtctc gaccggctga tgatcgaact tgatggcacg gagaacaagg gccggctcgg
     1921 cgccaacgcg atcctcggcg tttcgctcgc cgccgcccat gcctcggccg cgatggaaaa
     1981 gcgcccgctc tacagctacc tgggcgacct ctacggggag tccgatccgt cgctgctgcc
     2041 ggtcccgatg atgaacgtcc tgaacggcgg cgcccacgcg gacaattcgg tcgacttcca
     2101 ggagttcatg atcatgcccg cgggaactcc gtccttctcc gaatgcctgc gggtcgggac
     2161 cgaggtcttc cattcgctga agaagattct cgccggtcac ggtctggcca ccgcggtcgg
     2221 tgacgagggc ggattcgccc ccgacctttc ctccaacgag gccgccctcg agatgctgat
     2281 cgaaggcatc gaggcagccg gttacaagcc gggagaggaa gtcttcatcg ctctcgaccc
     2341 ggccacctcc gagatctact cggacggtgt ctaccgcctc gagcacgagg accgggacct
     2401 cagcgcgtcc gaga
//
LOCUS       DAOTIP010000304         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51964_length_2414_cov_2.918186, whole genome shotgun sequence.
ACCESSION   DAOTIP010000304 DAOTIP010000000
VERSION     DAOTIP010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51964_length_2414_cov_2.918186"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="P5138_06445"
     CDS             <1..182
                     /locus_tag="P5138_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HRV60252.1"
                     /translation="PAHLKGSLTGFDLTLPVRSGRFHLGTWQGIYLCEHRDRGGSRSL
                     TATGWGQASRTNILD"
     gene            182..586
                     /gene="nusB"
                     /locus_tag="P5138_06450"
     CDS             182..586
                     /gene="nusB"
                     /locus_tag="P5138_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRV60253.1"
                     /translation="MRRSNQRRDAVFACYQQEVTDRPLSVLLAEAKPFTVELAEGVEA
                     HRDELDEVISEYAQGWDLKRIAPLEKNIMRVALYEMNFRDDVPYEVAIDEAVETAKKF
                     CGAEAPGFVNGILGAAAREMKSDAEETNGEAA"
     gene            583..756
                     /locus_tag="P5138_06455"
     CDS             583..756
                     /locus_tag="P5138_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60254.1"
                     /translation="MSRLEDIRDRLDEITAALRDENVSDTDAAELAGEAARLTAEAAS
                     EAATAVERADRQG"
     gene            820..1671
                     /locus_tag="P5138_06460"
     CDS             820..1671
                     /locus_tag="P5138_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRV60255.1"
                     /translation="MVDRHLETVSFSHGAGPDGADTTGLDEAMRYSLLAGGKRIRPVL
                     CLATARSIGEPLEDFLGAAAALEMIHTYSLVHDDLPAMDDDDLRRGKPTSHVKYGEAV
                     AILAGDGLFAEAMGALATAPAPAGRVLEAVSLLSAATGVEGMVGGQYIDVSAQTLDAD
                     GLRHLHSLKTGRLIRASVEVALALGGLDEAGREPYRRFASLIGLLFQIVDDILDVTGS
                     DEELGKPRGSDERHGKVTYVSLYGLERARALGAETHQQALAALAGADGETDDLRRIAD
                     YIYSREQ"
     gene            1668..>2414
                     /locus_tag="P5138_06465"
     CDS             1668..>2414
                     /locus_tag="P5138_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRV60256.1"
                     /translation="MTEANDDMTPLLPGIDSPSDLKGLSDEQLQQVADETRAYIIDVI
                     GEIGGHFGANLGTCEIAVALHSLLESPRDKVLWDVGHQAYPHKVLTGRREELPTIRKY
                     KGLAPFCSIPESEHDIMGAGHASTSISYALGLKEAMRNGIGEDGKVVAVIGDGALTGG
                     VAYEAMHAAGGLQTPMVILLNDNGMSISPNVGALSSYFQRARLKPGLFHAREEVEDRL
                     TRLPLGLGKRIERLGPELKSAIKAYWAPGLL"
BASE COUNT          441 a          784 c          820 g          369 t
ORIGIN      
        1 tgcccgccca cctgaagggt tcgctcaccg gtttcgacct gaccctgccg gtccggtccg
       61 gccggtttca cctcggtacc tggcagggga tctacctctg cgagcaccgc gaccggggcg
      121 gttccaggtc cctgacagca accggctggg gtcaggccag tcgaaccaat attcttgact
      181 gatgcgtcgc tccaatcagc gtcgggatgc ggttttcgcc tgctaccagc aggaggtgac
      241 cgaccggccc ctgtccgtcc tcctcgccga ggcgaagcct ttcaccgtgg agctggccga
      301 aggggtggag gcgcaccgcg acgaactcga cgaggtcatt tccgagtacg cgcagggctg
      361 ggacctcaag aggatcgctc cgctcgagaa gaacatcatg cgggtagccc tctacgagat
      421 gaacttccgg gatgacgtgc cctacgaggt ggcgatcgac gaagcggtcg agaccgccaa
      481 gaaattctgc ggtgccgagg cccctggctt cgtcaacggg atcctcgggg cggccgcccg
      541 cgagatgaag tcggacgcgg aagagacaaa cggggaggcc gcgtgagcag gctcgaggac
      601 atccgggacc ggctcgacga gatcacggcc gcccttcgtg acgaaaacgt ctccgacacc
      661 gacgcggccg aactggccgg tgaagcggcc agactgaccg cggaagctgc aagcgaagcg
      721 gccacggccg tcgagcgtgc cgaccggcag ggctgagccg atgagagtca tccagcggga
      781 cggcggcgag ccttgagcta ccccgaagac cttcgcgaaa tggtggatcg ccacctggag
      841 acggtcagct tcagccacgg agccgggccg gacggggccg acacgaccgg gctcgacgaa
      901 gcgatgcgct attcgctgct ggccggcggc aagcggatcc gcccggtgct ctgcctggcg
      961 acggcacgat cgatcggtga gccgctggag gactttctcg gagcggcggc ggcgctcgag
     1021 atgattcaca cctattcgct ggtgcacgac gacctgcccg cgatggacga cgacgatctc
     1081 agacgaggca agccgacctc tcacgtcaag tacggcgagg cggtggcgat ccttgccggc
     1141 gacggactgt tcgcggaggc gatgggcgcg ctcgccacgg ccccggcccc ggccggccgg
     1201 gtgctggagg cggtcagcct cctcagcgcc gccaccgggg tcgagggaat ggtcggcggc
     1261 cagtacatag acgtatcggc ccagaccctg gacgccgatg gtctgaggca ccttcattca
     1321 ctgaagaccg gcaggctgat ccgggccagc gtggaagttg ccctcgcact ggggggtctc
     1381 gacgaagccg gtcgcgagcc gtaccggaga tttgcctcgc tgatcggcct gcttttccag
     1441 atcgtcgacg acatcctcga cgtcaccgga agcgacgagg agcttggcaa gccccggggc
     1501 agcgacgagc gccacgggaa ggtcacatat gtcagcctgt atggattgga acgggcccga
     1561 gcgctgggcg ccgaaaccca ccagcaggct ctcgccgccc tggccggtgc cgacggcgaa
     1621 accgacgacc tcaggcggat cgccgactac atttactcga gagaacagtg accgaagcca
     1681 acgacgacat gactccgctg ctccccggta tcgactcacc ctcggatctc aagggcctga
     1741 gcgacgagca gctccagcag gtggctgacg aaacccgcgc ctacatcatc gatgtgatcg
     1801 gtgagatcgg cggccacttc ggggcgaacc tgggtacctg cgagatcgcg gtcgcactcc
     1861 acagcctgct cgaatcccct cgcgacaagg ttctctggga cgtcgggcat caggcctatc
     1921 cgcacaaggt cctgaccggc cggcgggaag aactgccgac catccgaaag tacaaggggc
     1981 tcgccccgtt ctgttcgatt cccgagtcgg agcacgacat catgggtgcc ggccacgcct
     2041 cgacctcgat ttcctacgcc ctcggcctga aggaggcgat gcggaacggg atcggcgagg
     2101 acggcaaggt ggtcgcggtg atcggcgatg gcgccctgac cggtggagtt gcctatgagg
     2161 cgatgcatgc ggcgggcggg ctccagacgc cgatggtgat cctgctcaac gacaacggca
     2221 tgtcgatttc gccgaatgtc ggtgctctct ccagctactt ccagcgggcc cgcctgaagc
     2281 ccggcctttt ccacgcccgg gaagaggtcg aggaccggct gaccaggctt ccgctcgggc
     2341 tcggcaagcg gatcgagagg ctggggccgg agctcaagtc cgcgatcaag gcctactggg
     2401 ctccgggact gctc
//
LOCUS       DAOTIP010000305         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_51988_length_2413_cov_3.062341, whole genome shotgun sequence.
ACCESSION   DAOTIP010000305 DAOTIP010000000
VERSION     DAOTIP010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51988_length_2413_cov_3.062341"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="P5138_06470"
     CDS             <1..1696
                     /locus_tag="P5138_06470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRV60257.1"
                     /translation="KFAAEYEGKVSELDGPGLIVAMTKLADISDRAGRALNYAHLRFA
                     ADTADPANGALLQMGSERATRIQTQLLFFDLEWAALDDEVAEALLATPGLEFCEHHLR
                     NERKYRDHLLSAPEEKIVTELSVTGAGAWSRLFDELTSAIRVNLPGGEEPVSLDIALS
                     GLHNPDREVRKATAEAVTESLEPGLRTRAYVFNTLLQNKATMDRLREYPHWLATRNLS
                     NEASDESVQALVQAVKGRYDLARSWYRTKAKLLEIDRLADYDRMAAIATDDVTIEWEE
                     GRQIVQSAFDSLAPQAGEVVGRFFEDRWIDAPPAPSKRGGAFSASTVPSVHPYVMLNY
                     TDRRRDVLTLAHELGHGLHQALAAKQGIFHQDTPLTVAETASVFAEELVFGRLLAAEK
                     DPASRLGLLSESVEGQIATIFRQIAMNQFEDAVHTARREEGELSVERFGELWAATQED
                     LLGDSVEVTDGYRSWWSYVPHFIGSPGYVYAYAYGQLLALSVYRLYEEQGEAIVPGYL
                     EMLTAGGSRSPEQLGALVGVDLSDPSFWDNGLDLVAGQIDAAQDAAEEVLRARSQG"
     gene            1810..2361
                     /locus_tag="P5138_06475"
     CDS             1810..2361
                     /locus_tag="P5138_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV60258.1"
                     /translation="MPEQSRKPRVSQEARRAEVIDAALIEFGRCGYAGTSTSMIANRA
                     GIRQPYIYALFENKRALFLACHEVLNDRIRETFRNAALPEDSPYERVRKMGLAYLGLL
                     HDDDRVRCHLQVFAAAGSDDLKEPIREGFNRLFEDVLEMSEASRPEVARFFATGMLLN
                     AMAALDEPFEMIRYLEVTPEDEL"
BASE COUNT          439 a          801 c          768 g          405 t
ORIGIN      
        1 gaagttcgca gccgagtacg aaggcaaggt ctccgagctg gacggtcccg gcctgatcgt
       61 cgccatgacc aaactggccg acatctccga ccgggccggc cgggcgctca actacgccca
      121 cctgcgcttc gcggccgaca ccgccgatcc ggccaacggg gccctgctcc agatgggctc
      181 ggagcgggcg acccggatcc agacccagct gctcttcttc gacctcgagt gggccgccct
      241 cgatgacgag gtggccgaag cgctgctggc cacaccgggt ctcgagttct gcgagcacca
      301 cctgagaaac gagcgcaagt accgcgacca tctgctgagc gccccggaag aaaagatcgt
      361 gaccgaactc tcggtcacgg gcgccggagc ctggtcccgg ctcttcgacg agctgacctc
      421 ggcgatccgg gtcaacctgc ccggcggaga ggaaccggtc agtctcgaca tcgccctttc
      481 gggacttcac aaccccgacc gtgaagtccg caaggcgacg gccgaggcgg tgaccgagtc
      541 gctggaaccc gggctaagga cgcgggccta cgtcttcaac accctgcttc agaacaaggc
      601 gaccatggac cggctgcgtg agtatccgca ttggctggcc acacgcaacc tcagcaacga
      661 agcttccgac gaatcggtcc aggccctggt ccaggcggtg aagggccgct acgacctcgc
      721 ccgttcctgg taccggacca aggcgaaact gctggaaatc gatcgtcttg ccgactacga
      781 ccgcatggcg gcgatcgcca ccgatgacgt gacgatcgag tgggaggaag gccgccagat
      841 cgtccagagc gccttcgatt cgcttgcccc gcaggccggt gaggtcgtgg gacgcttctt
      901 cgaggaccgc tggattgacg cgccgccggc cccgagcaag cggggcggcg ccttctcggc
      961 ttcgacggtg ccttcggttc atccctatgt gatgctgaac tacaccgacc ggcgccggga
     1021 cgtgctcacg ctggcccatg agctcggcca cggccttcac caggcgctgg cggcaaagca
     1081 gggcatcttc caccaggaca cgccgctgac cgtggccgaa actgcttcgg tcttcgccga
     1141 ggaactggtc ttcggccggt tgctggcggc cgagaaggat ccggcttccc ggctcggcct
     1201 gctttccgaa tcggtcgaag gccagatcgc gacgatcttc cggcagatcg cgatgaacca
     1261 gttcgaggac gcggtccaca ccgcccgccg cgaggaaggc gagctttcgg ttgagcggtt
     1321 cggcgagctc tgggccgcga cccaggagga tctgctcgga gattcggtcg aagtgaccga
     1381 cggctaccgc tcctggtggt cgtatgtgcc ccacttcatc gggtccccgg gttacgtcta
     1441 cgcctacgcc tacggccagc tgctcgcgct ctcggtctac cgcctctacg aggaacaggg
     1501 cgaagcgatc gtgcccggtt atctggagat gctgaccgcc ggcggctccc gttccccaga
     1561 gcaactcggg gcgctggtcg gggttgatct cagtgacccc tcgttctggg acaacggatt
     1621 ggatctggtg gcaggacaga tcgacgcagc gcaggacgca gcagaagaag tgcttcgggc
     1681 ccgaagccag ggttgatcga catctgaccc gtcttttgca ccggcgctct ttccggcgga
     1741 ccggccgaag ggtgcgaaaa cattggatcc gggtaaagga cggttacccg gttgaccgaa
     1801 gggccaggga tgccagagca atcgagaaaa ccgagggttt cacaggaagc tcggcgagcc
     1861 gaggtcatcg acgcggccct gatcgagttc ggccgctgcg gctacgccgg cacttcaacc
     1921 tcgatgattg ccaaccgggc cggaatccgc cagccgtata tctacgccct gttcgagaac
     1981 aagcgggccc tctttctcgc ctgtcatgaa gttctcaacg accggatccg ggaaactttc
     2041 cggaacgcgg ccctgcccga ggacagtccc tacgaacgag tccggaagat gggactcgcc
     2101 tatctcggcc tgcttcacga tgacgaccgg gtccgctgcc acctccaggt ctttgccgcg
     2161 gccggcagcg acgatctgaa ggaaccgatc cgggaagggt tcaaccggct ctttgaagac
     2221 gtgctcgaga tgagcgaggc gagccggccc gaagtggccc gcttcttcgc gaccggcatg
     2281 ctgctcaacg cgatggctgc cctggacgaa ccgttcgaga tgatccgcta tctggaagtc
     2341 accccggagg acgaactttg accggccctc accccagact cacggccctg ctggtcaccg
     2401 ggatcctgct ctt
//
LOCUS       DAOTIP010000306         2411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52042_length_2411_cov_2.981324, whole genome shotgun sequence.
ACCESSION   DAOTIP010000306 DAOTIP010000000
VERSION     DAOTIP010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52042_length_2411_cov_2.981324"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="P5138_06480"
     CDS             <1..1180
                     /locus_tag="P5138_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60259.1"
                     /translation="IRPGVSGSNLNDITTFGNFAYFSADNGLFGSEVWITDGNSASLF
                     ANINAGGLSSNPRYFTPFGDYLYFRAGDGQGAELWRTNGVFTQRVADINPGAPNSAPF
                     DLTVFNGYLYFRAEDGVSGTELWRTDGATPIANTALFADLEPGADGSSPTSLSVTGDR
                     LYFTADTAAMGRELWNTTGAGVDLVRDINPGEDHGSPGQLTGFMGSLYFSANDGTHGY
                     ELWEADGGQARMVGDILPGDDSSDPYDFEEHGGSLYFAADDGTTGYELWAATDDRKVK
                     VNLPGKKIRADRGGRIRIKVNCPANEISGLCRGNLTVQTRGKVRVGKKKRKVTLGKAK
                     FSVPAGQTRTVVVRLGRKARILVRRNAAARKVSVRAKVSDKAGNSRQVSKVKRLVVTR
                     "
     gene            1192..1926
                     /locus_tag="P5138_06485"
     CDS             1192..1926
                     /locus_tag="P5138_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HRV60260.1"
                     /translation="MIGPDDVQIRVVGCLIEKQRTTPDQYPLTLNSLRLACNQSTNRD
                     PVVDYDEATIRKALGDLGRRRWIRGASGHTSRSPKYRHLLDQELSLSEDQISVLSVLM
                     LRGEQTPGQLKQRTERMFGFHDLDALQTTLDALASRDLIERLPRRPGQKEDRYRQLIG
                     GNHLEETEADETRPVAPDPEGSSPADPEPVAPAPRGEAVEPLPVPQDTPETERIEGLE
                     RQVNELTRELAELRALVEDLREQLGA"
     gene            1974..>2411
                     /locus_tag="P5138_06490"
     CDS             1974..>2411
                     /locus_tag="P5138_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRV60261.1"
                     /translation="MAEPTEMKPEGVERDDSLLATMKRSGTEFMEDGMTDWAAALTYY
                     GLLSLFPMLIALVSIIGLFANPETTTEKLTEIVSNLGPDAATSTFQGPVEGLTSNRGA
                     SGVTFVIGLILAIWAASGYIGAFGRASNVIYETREGRAFWKLKP"
BASE COUNT          453 a          804 c          785 g          369 t
ORIGIN      
        1 cattcgcccc ggagtctccg gatcaaacct caacgacatc actacgtttg gcaacttcgc
       61 ctatttcagc gcagacaacg gcctgttcgg cagtgaagtg tggatcaccg acggcaacag
      121 cgcctccctt ttcgccaaca tcaacgcggg tggcctgagt tcgaatcccc ggtacttcac
      181 gcccttcggc gactatctct acttcagggc cggagacggc cagggggcgg agttgtggcg
      241 gaccaacggt gtgttcaccc agagagtcgc ggacatcaac cccggcgccc cgaattcggc
      301 tccgttcgac ttgaccgtat tcaacggata cctctatttc cgggccgagg acggggtgag
      361 cgggaccgaa ctctggcgca ccgatggcgc cacgccgatc gcaaacactg ccctcttcgc
      421 cgacctcgag cccggggcgg acggctcctc gccgacgagc ctgtccgtga ccggcgaccg
      481 gctctacttc acggctgaca cggcggccat ggggcgcgag ctctggaata cgaccggagc
      541 cggggtggac ctggtccggg acataaaccc gggtgaagac catggcagcc cgggtcaatt
      601 gaccggattc atgggcagcc tctacttctc ggccaacgac ggcacgcacg ggtacgagct
      661 atgggaggcg gatggcggcc aggccaggat ggtcggggac atcctgccgg gagacgattc
      721 cagcgaccca tatgacttcg aggagcatgg gggcagcctc tatttcgcgg ccgacgacgg
      781 gaccaccggc tacgaactct gggcggctac cgatgacagg aaggtgaagg taaatctgcc
      841 cggcaagaag atcagggcag accggggcgg ccggatcagg atcaaggtca actgcccggc
      901 gaacgagatc tccggtctct gtcgcggcaa cctgaccgtc cagacccgcg gcaaggtgag
      961 ggtcggaaag aagaagcgca aggtcacgct cggcaaggcg aagttctcgg tcccggccgg
     1021 acagacgcgg acggtggtcg tccggctggg ccggaaggcc cgcatcctgg tccgccgcaa
     1081 cgccgcagcc cggaaggtct cggtgcgggc caaagtcagt gacaaggccg gcaactcccg
     1141 ccaggtaagc aaggtgaagc ggctggtcgt gacccggtag cctccgccgc gatgatcgga
     1201 ccggatgacg ttcagatccg ggtggtcggc tgcctgatcg agaagcagcg gaccactccg
     1261 gatcagtacc ccctgaccct gaactcgctg cggcttgcct gcaaccagtc gaccaaccgc
     1321 gaccccgtgg tcgactacga cgaggcgacg atccgcaagg ccctgggcga cctgggccgg
     1381 cgccggtgga tccgcggggc atccgggcat accagccgtt cccccaagta ccggcacctg
     1441 ctggaccagg aactctcact ttccgaggac cagatctcgg ttctgtctgt gctcatgctc
     1501 cgcggcgagc agacgcccgg gcagctcaag cagcgcaccg agcgtatgtt cggcttccac
     1561 gatctcgacg ccttgcagac cacgctcgat gccctcgcct cgagggatct gatcgagcgg
     1621 ctgccccgtc gcccgggcca gaaggaggac cgctaccggc agctgatcgg cggcaaccac
     1681 ctcgaggaga cggaagcgga cgagacccgg cccgtcgccc cggatcccga gggcagctcg
     1741 ccggcggatc ccgagccggt cgccccggct ccccgggggg aggcggtcga acccctgccg
     1801 gtcccgcagg acacgccaga gaccgaacgg atcgaagggc tggagcgtca ggtcaacgag
     1861 ttgactcgtg agctcgccga acttcgggct ctggtcgagg acctgcgcga acaactcggc
     1921 gcctgatccc ggcgggaatc gttcgaattc attgctccgg gtacaagtga gtcatggctg
     1981 agccgaccga gatgaaaccc gagggagtcg aacgggacga ctcgctgctc gcgaccatga
     2041 agcgcagcgg caccgagttc atggaagacg ggatgaccga ctgggccgct gccctcacct
     2101 actacggcct gctctcgctg tttccgatgc tgatcgccct ggtctcgatc atcgggctgt
     2161 tcgcgaaccc ggagaccaca accgagaagc tgaccgagat cgtttccaac ctgggtcccg
     2221 acgccgcgac cagcaccttc cagggcccgg tcgagggtct cacctcgaac cggggagcat
     2281 ccggcgtcac cttcgtgatc ggcctcatcc tggcgatctg ggccgcctcc ggctacatcg
     2341 gcgccttcgg ccgggcgtcg aacgtgatct acgagacccg cgaaggccgg gccttctgga
     2401 agctcaagcc g
//
LOCUS       DAOTIP010000307         2406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52189_length_2406_cov_2.759251, whole genome shotgun sequence.
ACCESSION   DAOTIP010000307 DAOTIP010000000
VERSION     DAOTIP010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52189_length_2406_cov_2.759251"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="P5138_06495"
     CDS             <1..339
                     /locus_tag="P5138_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60262.1"
                     /translation="QLRILPGAGVFVGETAADYMNVDLGFGLTNTTYAIGALLIVTLI
                     FQFRSKRYVPGIYWTAVVLISVVGTLISDNLVDNMGCPLKPRPLHFQRPWRSLSSSGT
                     HSKEPSRSTR"
     gene            240..755
                     /locus_tag="P5138_06500"
     CDS             240..755
                     /locus_tag="P5138_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60263.1"
                     /translation="MPLETTTIAFSAALAITFIVWYAFERTLSIHSITTTRREAFYWL
                     AILFTFALGTSAGDQIAEQYGLGYFVSILIFAGAIALVALAHFRFRLGAITAFWAAYI
                     LTRPLGASIGDWMSQPTSDGGLGLGTTDTSFIFLAAILGLVIYLSVTKVDQTPPELVE
                     AEMEEAEATHT"
     gene            complement(808..1632)
                     /locus_tag="P5138_06505"
     CDS             complement(808..1632)
                     /locus_tag="P5138_06505"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRV60264.1"
                     /translation="MNFLQSIILGLLQGLTEFLPISSSGHLLIMPAILGWDDPGAAFT
                     ATIQLGTEAAVLIYFRKDLWRIARTWIVSWSDRDLRRSPDARMGWYLVIATVPIALLG
                     FAFQNLIETAARNLWLVSGTLIVFGLVLGLADRIGARTRRTEELSFRDGALIGVAQSL
                     ALIPGVSRSGATISAGLFLGLQREAAARFSFLLAIPAVVLSGTYELLKIVTGKEHVAE
                     PLLNVAVATAVAFVVGMAVIAWFLRYLTTHSLKIFVWYRIGLGCLVLVLLSAGAIS"
     gene            complement(1714..>2406)
                     /locus_tag="P5138_06510"
     CDS             complement(1714..>2406)
                     /locus_tag="P5138_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV60265.1"
                     /translation="AYMVRHERNFSAELSHELRTPLSAISAEAEIALRKSRNPDQYRK
                     SLERISEQAKDLTRILETLLDVARSESSAVPEETVDLFDSIEAAATGTRSLADQYGIA
                     IELERPLTDPKAQLAGDKFQRMIAPILENAVTYARSSVSISVLTSSRMAQVIVSDDGP
                     GFTREDIESAFEPGVRGSARRNQAAPAGTGLGLALARRLARANGGEVSIGEAAGRTEV
                     TLSVPLALGSAT"
BASE COUNT          486 a          744 c          715 g          461 t
ORIGIN      
        1 caattgcgta tcctgccggg cgccggggtg ttcgttggtg aaaccgccgc cgactacatg
       61 aacgtcgacc tgggtttcgg tctgaccaac accacctatg cgataggcgc cctgctcatc
      121 gtcactttga ttttccagtt caggtccaag cgctatgtac cggggatcta ctggaccgcc
      181 gtggtcctga tcagcgtggt cggcaccctg atatccgaca accttgtcga caacatgggg
      241 tgccccttga aaccacgacc attgcatttt cagcggccct ggcgatcact ttcatcgtct
      301 ggtacgcatt cgaaagaacc ctctcgatcc actcgataac cacaaccaga cgggaagcct
      361 tctactggct ggcgatcctc ttcacctttg cccttggaac ctcggccggt gaccagattg
      421 ccgagcaata cggcctgggt tacttcgttt cgatcctcat ttttgccgga gcgatcgcgc
      481 tcgtcgcact cgctcatttc aggttcaggc ttggagcgat cactgctttc tgggccgcct
      541 acatcctgac ccggccactc ggcgcatcga tcggcgactg gatgtcgcaa ccgacctcag
      601 acggcgggct cgggctgggc accaccgaca ccagcttcat attcctcgca gcgattctcg
      661 gtctcgtcat atacctatcg gtaaccaagg tcgaccagac cccgccggaa ctcgtcgagg
      721 ccgagatgga ggaagccgaa gccacccaca cctgatcgac ttgcccgggg cgtcgtccgc
      781 aagtccgggc ggcgcgccgg gtgaatgtca ggaaatcgcc cccgcggaaa gcaggaccag
      841 gacgaggcag cccagtccga tccggtacca gacgaagatc ttcagcgaat gggtggtgag
      901 atagcggagg aaccaggcga ttactgccat gccaaccacg aacgcgacgg ccgtcgcaac
      961 cgccacgttc agcagcggtt cggcgacatg ttccttgccg gtgacgatct tcagcaactc
     1021 gtaggtaccg gacaacacca ccgccggaat ggcgaggagg aacgagaacc tcgcggccgc
     1081 ttcgcgctga aggccgagaa agagtccggc gctaatcgtc gcccccgatc gggagactcc
     1141 cggaatcaat gcgaggctct gcgcaacccc gatcagggca ccgtcacgaa acgagagttc
     1201 ttcagttctt ctggtgcggg cgccgatccg gtcggccagg ccaagtacga gaccgaagac
     1261 gatcagggtt cctgatacga gccaaaggtt ccgcgcagcg gtctcgatca gattctggaa
     1321 ggcgaatccc agcaacgcga tcggaaccgt tgcgataacc agataccacc ccatccgagc
     1381 gtcagggctc ctgcgcaggt cccggtcgga ccaggaaact atccaggtcc gggcgatcct
     1441 ccagagatcc tttcgaaagt agatcaggac ggcggcctcg gtacccagct gaatggttgc
     1501 ggtgaaggct gctccggggt catcccatcc cagaatcgcc ggcatgatca gaagatgtcc
     1561 gctggacgaa atcggaagaa actcggtcag gccctgaagc agaccgagaa tgatgctctg
     1621 aaggaagttc aagccctaac cgggttgcga agcatgttca ggtatggcca tgaccggaga
     1681 ttgacacagc acttctgaac attttctgaa cggtcaggtg gccgaaccca atgccagcgg
     1741 gaccgacaag gtgacctcgg tgcgaccggc tgcttccccg atcgagacct cgccgccgtt
     1801 ggcccgcgcg agtcgtctgg cgagagcaag gccgaggccg gttccggccg gagcggcctg
     1861 gtttcgcctg gcagaccccc gaacccctgg ctcgaaggca ctctcgatat cttcgcgagt
     1921 gaaccccgga ccgtcatcgc tgacgatcac ttgggccatt cgggaggaag tcaggaccga
     1981 gatcgatact gaactccgcg cgtaggtgac cgcgttctcg aggatcgggg caatcattcg
     2041 ctgaaacttg tccccagcca gttgtgcctt cgggtctgtg agagggcgtt caagttcgat
     2101 cgcgattccg tactggtctg cgagggatct ggtcccggtg gcggctgcct cgattgaatc
     2161 gaaaaggtcg accgtctcct cggggacagc gctgctctcg gaccgggcga catccagcag
     2221 ggtctcaaga atcctcgtca agtctttcgc ctgttcggag atcctttcga gcgatttccg
     2281 gtactggtcc gggtttcggc tcttccgaag ggcgatctcc gcttcggcgg agatcgcaga
     2341 gaggggggtc ctgagctcgt gcgaaagctc tgcggaaaag ttccgctcgt gtcggaccat
     2401 gtacgc
//
LOCUS       DAOTIP010000308         2405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52218_length_2405_cov_2.805106, whole genome shotgun sequence.
ACCESSION   DAOTIP010000308 DAOTIP010000000
VERSION     DAOTIP010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52218_length_2405_cov_2.805106"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>2405
                     /locus_tag="P5138_06515"
     CDS             64..>2405
                     /locus_tag="P5138_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRV60266.1"
                     /translation="MSCTLNKNPNYFKSDKGWFDRVEFLAIKDVTARTNALNSGETHY
                     LHRADLKTLDLLLRREVIADSYREQFAEIMVDEFQDTNRLQMDLIDKLRGEETTLFTV
                     GDEMQAIYGFRYADVELYRQRRAGISFRLPADAERRAAGERDRVTFEVTTLPLSANFR
                     SQAPVIGAVNELGNQFESLIEGIRTDDEPGGETGGTRHEFEHLRVGWSPPEPGSGPAP
                     PPEPKVEILLTKAKAWQQVDLGPLSPAPTGDQYMTESRGQHEAEALALARHIRKAVDD
                     GIPPGDIVVLLRTKTRMWLFEAALKQAGLRPYVVGGTGFWETREGIDMRALLATVANP
                     LDDEALAGALAGPACGLSPSALWLLGSRRDHDQPLWPLVSNLADKPDPEISEEDNRRA
                     GEFVATIRRLRAKLAGLPLSAVVQTAVSETGYDLVNLMRDPSGSGLANIRRVAEIASE
                     FEATEGRDLRGLIEWIDDSARLDSEQAVATEDEESDVVRLMTIHKSKGLEFPMVCVAD
                     LGLASKADSENVIWISPRNDDPGKFEVGLRLPEPGGPGLDLFAWQTLKDQARTDKTDE
                     ELRILHVAMTRAESRLVLSGVTDFEKIPDPTEANPFATRLTQAYGVEDFPGTIPVPAP
                     DFLVAPAETPAISIEVILNRPDDAGDLAEVTSPESLNRADASTGEPPIGRPVSPGFPE
                     VPLSYSALASYRECPARFFATRVLKLDEPERRHGDAKQPLDPEMEIRKEIDATSFGVA
                     VHELLELLPARRWIRPADAEIDSRLEANGVPGADAGDLAR"
BASE COUNT          461 a          781 c          783 g          380 t
ORIGIN      
        1 tcggtcgatt gggagtcggg caaccgcacc ggaccctaca cactcgaaaa attcgaacct
       61 ggcgtgagtt gcaccctcaa caagaatccg aactacttca agtccgacaa gggctggttc
      121 gaccgggttg agttcctcgc aatcaaggat gtcacggcgc gcaccaacgc cctgaactcc
      181 ggcgaaaccc actacctgca ccgggccgat ctcaagactc tcgatctgct ccttaggcgc
      241 gaggtgatcg cggattccta ccgcgagcag ttcgccgaga tcatggtcga cgagttccag
      301 gacacgaacc gcctgcagat ggatctgatc gacaagctcc gtggcgaaga gacgaccctg
      361 ttcacggtcg gcgacgagat gcaggcgatt tacggattcc gctatgccga cgtcgagctc
      421 taccggcagc ggcgtgccgg catctcgttc cggcttcccg ccgatgccga acgccgcgcg
      481 gccggtgagc gggaccgggt cactttcgag gtcacgactc tcccgctgag cgcaaacttc
      541 cgctcccagg ccccggtgat cggtgcggtc aacgagctcg gcaaccagtt cgaatcacta
      601 atcgagggca tcaggaccga cgatgaaccg ggcggagaga ccggtggcac ccgccacgag
      661 ttcgagcatc tcagggtcgg ctggtcaccg ccggagccgg gctccgggcc ggccccgccc
      721 cccgaaccga aggtcgagat ccttctgacc aaagccaagg cctggcagca ggttgatctc
      781 ggaccgctct caccggcgcc gaccggagac cagtacatga ccgagagccg cgggcagcac
      841 gaagccgaag cgctggccct ggcccgtcac attcgcaagg ccgtagacga cgggataccg
      901 cccggcgaca tcgtcgttct gctcaggacc aagacgagaa tgtggctctt cgaagccgcg
      961 ctcaagcagg ccggactgag gccctacgtg gtcgggggca cgggtttctg ggagacccgc
     1021 gaaggcattg acatgagggc gttgctggcg accgtcgcga acccgctcga tgacgaggcg
     1081 ctggccggcg ctttggccgg tccggcctgc gggctcagcc cgagtgcact ctggttgctc
     1141 ggcagccgtc gcgatcacga tcagccgctc tggccgctgg tctcgaacct ggcggacaag
     1201 ccggatccgg agatctccga ggaagacaac cggcgggccg gcgagttcgt ggcgaccatc
     1261 cggcgccttc gcgcgaagct ggcgggcttg ccgctttcag ccgtggtcca gaccgcggtt
     1321 tcggaaaccg gctacgacct cgtcaatctg atgcgggatc cgtctggttc cggactggcc
     1381 aacatccggc gggtggccga gatcgccagc gagttcgaag cgacagaggg cagggatctg
     1441 cgggggctga tcgagtggat cgacgacagc gcgcgactgg actcggagca ggcggtggca
     1501 accgaagacg aagagtcgga cgtggtacgc ctgatgacga tccacaagtc gaagggtctt
     1561 gagttcccga tggtttgcgt cgccgacctc ggtctggcct cgaaggccga ttccgaaaac
     1621 gtgatctgga tctcgccacg gaacgacgat ccgggcaagt tcgaggtcgg actgcgactg
     1681 cccgaaccgg gaggacccgg gcttgatctg ttcgcctggc aaacgcttaa ggatcaagcc
     1741 aggacagaca agaccgacga agaactgcgg atcctccacg tcgccatgac cagagccgag
     1801 agccggctcg tgttgagcgg cgtcaccgac ttcgagaaga tccctgaccc gaccgaagcc
     1861 aacccttttg cgacccgcct gacccaggcc tacggggtcg aagacttccc gggcaccatc
     1921 cctgtgccgg ccccggattt cctggtcgca ccggctgaaa cccccgcgat ctcgatcgag
     1981 gtgattctga accggccgga cgatgccggg gatctggccg aagtgaccag tccggaaagc
     2041 ctgaaccggg cagatgcgtc taccggcgag ccgccaattg gccggccggt atctcccggt
     2101 tttccggaag tcccgctctc ctactccgcc cttgcctcct accgggagtg tcccgcccgg
     2161 ttcttcgcca ccagggtgct gaagctggac gaaccggaac gccggcacgg cgacgcgaag
     2221 caaccgctgg atccggaaat ggagatccgc aaggagatcg atgccacctc attcggggtg
     2281 gcggtgcacg agctgctgga gctgctgccc gcacgccgct ggatcaggcc ggccgatgcc
     2341 gaaatcgact cgagactgga ggcgaacggg gttccgggag cagatgccgg cgacctggcc
     2401 cggga
//
LOCUS       DAOTIP010000309         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52387_length_2400_cov_2.586780, whole genome shotgun sequence.
ACCESSION   DAOTIP010000309 DAOTIP010000000
VERSION     DAOTIP010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52387_length_2400_cov_2.586780"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="P5138_06520"
     CDS             <1..1273
                     /locus_tag="P5138_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV60267.1"
                     /translation="CLKPQPWVRQEWADEYGLEDVNTPDFDRHIDSVWQRIKVNDDCS
                     ELNQTQKTWIDAAEKLGWSWHKTNRNWDPEKHDPKVAGLMGWGDQSGAKQSTLKTYLE
                     DAVGNGAEVIVGCQAEKVLVESGRAAGVEANFGGAKVTVRAPRVVVAGGALESPALLL
                     RSGIGGQATGKYLRLHPCTLIFATYPEDQQAWWGPPHAAVVDEFDQGLENDGYGFLIE
                     GAQYTTGLGGSAIPFTTAEQHRELMAGYANGSITINLTRERGGGQVLIDGDGQSMPIY
                     SMTDPRDIANMHFGLEQQARLHRAAGAGQIFALATGMPGWRVGDDFEAFVERIHRIPL
                     RAGGAKLFAAHQMGSCRMGSDPAESVADPRGELHDTPGVWIGDGSAFPTPSGTNPMIT
                     IMSLASRTAEKILEDAGADGRETSKTVEAGA"
     gene            1270..>2400
                     /locus_tag="P5138_06525"
     CDS             1270..>2400
                     /locus_tag="P5138_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV60268.1"
                     /translation="MNPTEAPVGTEEATDTGTDQTAEAPTWAAKCGSHFIGGQWREPV
                     SSDVIEVINAVTEEVMGTVPAGTAVDVDFAVEAARAALPGWKALPVQDRAGFCTLIGH
                     GLQDRAEEIAGLISQELGTPVTEGMLIQAALPAIDFTSMEHLIEEMPWEQTIGNSKVV
                     REPLGVVGAITPWNYPIHQIAAKVAPAITAGCTVVLKPSEMTPLAAYLLMEVIESVGL
                     PGGVVNMVCGTGPVVGEAIASHPGIDMVSFTGSTRAGVRVSELAAASVKPVTVELGGK
                     SANVILDDADFEAAVVNGIQKCFINGGQTCSALTRMVVPRESLPVVEAVAAAAIEQIK
                     VGDPTDPETEIGPMSSAAQRDRVRGYIEKGVEEGATLVAGGSE"
BASE COUNT          423 a          814 c          832 g          331 t
ORIGIN      
        1 ctgcctcaag ccgcagccgt gggtccggca ggaatgggcc gacgagtacg gactcgaaga
       61 cgtgaacacc cccgacttcg accggcacat cgactcggtc tggcagcgga tcaaggtcaa
      121 cgacgactgc tcggagctga accagaccca gaagacctgg atcgacgcgg ccgagaagct
      181 cggctggagc tggcacaaga ccaaccgcaa ctgggacccg gagaagcatg acccgaaggt
      241 cgccggactg atgggctggg gtgatcagtc cggggccaag cagtccacgc tgaagaccta
      301 cctcgaggac gcggtcggaa acggcgccga ggtgatcgtc ggctgccagg ccgagaaggt
      361 cctggtcgaa agcggccggg cagccggggt cgaggcgaac ttcggtggag cgaaggtgac
      421 ggtccgcgcc ccgcgggttg tggtcgccgg cggcgcgctt gagtcccctg ccctgcttct
      481 ccgttccggc atcggcgggc aggcgaccgg caagtacctg cgcctccacc cctgcaccct
      541 gatcttcgcg acctaccccg aggaccagca ggcatggtgg ggtccgcccc acgccgcggt
      601 ggtcgacgag ttcgaccagg gcctcgagaa cgacggttac ggcttcctga tcgagggcgc
      661 ccagtacacg accggtctcg gcggctcggc gatccccttc acgaccgccg agcaacatcg
      721 ggagctgatg gccgggtacg cgaacggctc gataacgatc aacctgaccc gcgagcgtgg
      781 tggcggccag gtgctgatcg acggtgacgg ccagtcgatg ccgatctact cgatgaccga
      841 tccccgcgac atcgcgaaca tgcatttcgg cctcgagcag caggcccgcc tccatcgggc
      901 ggcgggagcc ggacagatct tcgccctggc gaccggcatg ccgggctggc gggtcggtga
      961 cgacttcgag gccttcgtcg aaaggatcca ccggatcccg ctcagggccg gcggagcgaa
     1021 gctgttcgcc gcccatcaga tgggctcctg ccggatgggc agcgacccgg ccgaaagcgt
     1081 ggccgacccc cgtggcgagc ttcacgacac gcccggggtc tggatcggcg acggctccgc
     1141 gttcccgacg ccttccggca ccaacccgat gatcacgatc atgtcgctcg cctcccgtac
     1201 cgccgagaag atcctcgagg acgcgggagc ggacggacga gaaaccagca agacagtgga
     1261 ggcaggagca tgaaccccac cgaagccccc gtcgggaccg aagaagccac ggacaccggc
     1321 accgatcaga ccgcggaggc gcccacctgg gctgcgaaat gcggcagcca cttcatcgga
     1381 ggccagtggc gcgagccggt ctccagcgac gtgatcgagg tgatcaacgc ggtcaccgaa
     1441 gaggtgatgg gaaccgttcc cgccggcacc gcggtggatg tcgacttcgc ggtcgaggcc
     1501 gcccgggccg ccctgcctgg ctggaaggcc cttccggtcc aggaccgggc cgggttctgc
     1561 accctgatcg gccacggcct ccaggaccgg gccgaggaga tcgccggtct gatcagccag
     1621 gagctcggca ccccggtgac cgagggcatg ctgattcagg ccgccctgcc ggcgatcgac
     1681 ttcacctcga tggagcacct gatcgaggag atgccctggg agcagacgat cggcaactcg
     1741 aaggtcgtcc gcgaaccgct cggcgtggtc ggcgcgatca ccccctggaa ctatccgatc
     1801 caccagatcg cggccaaggt cgccccggcg atcaccgccg gctgcaccgt ggtcctcaag
     1861 cccagcgaga tgaccccgct ggccgcctac ctgctgatgg aggtgatcga atcggtcggc
     1921 ctgcccggtg gcgtggtcaa catggtctgc ggcaccggcc cggtggtcgg cgaggcgatc
     1981 gcctcccacc cgggcatcga catggtctcc ttcaccggtt cgacccgggc cggcgtcagg
     2041 gtttcggagc tggcggcggc atcggtcaag ccggtcacgg tcgagctcgg cggcaagtcg
     2101 gccaacgtga tcctcgatga cgccgacttc gaggcggcgg tggtcaacgg catccagaag
     2161 tgcttcatca acggtggcca gacctgctcg gccctgaccc ggatggtggt tccccgcgaa
     2221 tcgctgccgg tcgtggaggc ggtcgccgcg gcggcgatcg agcagatcaa ggtcggcgac
     2281 ccgaccgatc ccgaaacaga gatcggcccg atgagctccg cagcccagcg cgaccgggtc
     2341 cgcgggtaca tcgagaaggg cgtcgaggaa ggcgcgacgc tggtcgctgg cggctccgag
//
LOCUS       DAOTIP010000310         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52909_length_2381_cov_3.420464, whole genome shotgun sequence.
ACCESSION   DAOTIP010000310 DAOTIP010000000
VERSION     DAOTIP010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52909_length_2381_cov_3.420464"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="P5138_06530"
     CDS             <1..389
                     /locus_tag="P5138_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRV60269.1"
                     /translation="EIRERMLAALHDQGFSDLTSAHMAVLRYPGPDGLKPVELATQTG
                     MTKQALNYLLAQLEEGGYLVRRGDPEDKRAKRLVMTEKGFEAGRVMRAEVLRIEKEYT
                     NAYGRKALDELKSHLVDLGRVLDRPD"
     gene            complement(410..>2381)
                     /locus_tag="P5138_06535"
     CDS             complement(410..>2381)
                     /locus_tag="P5138_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRV60270.1"
                     /translation="EIPGVKTSLGVSNVSFGVSQPARAVLNSVFLHHCVEAGLDLAMV
                     NPNHIKPYGEIPADEIELTEDLVFNRREDALERFIAHFESKGEEDEAEAEDAMAGMEP
                     EEALHFMILRRKKEGVEDWIDKSVEKIGAVPTLNEVLLPAMKEVGDKFGAGELILPFV
                     LQSAEVMKKAVSRLENYLDRIEGHTKGKVVIATVFGDVHDIGKSLVNTILTNNGYTVI
                     DLGKQVPVDTIIDAAIEHEADAIGLSALLVSTSKQMPIAVQELHQRGLEYPVLIGGAA
                     INRDFGRRTLYPHGKESDEIYEPGVIYCKDAFQGLDTMDALIDDGAREALIGRLKQEA
                     EDYRNKPVVVEDLPPLDDDSVRSSASTDAPIPTPPWWGVQEVPVDFDEVFDHLDLHVL
                     FKLHWGGKGVKGEEWDKLVSEEFRPRLERMWREQDYLHPKARLGYFPCNADGNELVIF
                     DPEDQDREINRLVFPRQPKHDRICLTDFYRPLNDEGIRDVVALQGVTVGPEVTELMEH
                     LEKDGEFAEQLYTHGLGVQSAEGLAEWLHYKVRQDLGIPSSQGRRYSWGYPACPDQSE
                     HTKVWELLDLGEIGMYLSDGYAVMPEQSTVAIIAHHPQAVYFGMKSGFIPKGEAPDEV
                     IAESDRSANPPEADPDEGPTEAEGEPAASAGA"
BASE COUNT          387 a          746 c          793 g          455 t
ORIGIN      
        1 acgaaatccg cgaaaggatg ctcgccgcgc tgcatgacca ggggttctcc gacctgactt
       61 cagcgcacat ggccgtgctc cggtaccccg gcccggatgg cctgaagccg gtcgagctgg
      121 cgacccagac cgggatgacg aagcaggcgc tcaactatct gctcgcccag ctggaagagg
      181 gcggctacct ggttcgccgt ggcgatcccg aagacaaacg ggcgaagcgg ctcgtgatga
      241 ccgaaaaggg atttgaggcc ggcagggtga tgagggcaga agtactcagg atcgagaagg
      301 agtacaccaa cgcctatggc cggaaagccc ttgacgagct gaaatcacat ctggttgatc
      361 tgggacgggt gctggatcga cccgactgat cgattgccaa agcgtcagtt caggcaccgg
      421 cggatgctgc cggttcgcct tccgcttccg tggggccttc gtcggggtct gcttcgggcg
      481 ggttggcgga gcggtccgat tcggcgatca cctcgtccgg ggcctctccc ttggggatga
      541 agccggactt catgccgaag tagaccgcct gggggtggtg agcgatgatc gcgacggtgg
      601 actgttcggg catcacggcg tagccgtcgc tcaggtacat gccgatctcg cccaggtcga
      661 gcagctccca gaccttggtg tgctcggact gatccgggca ggccgggtaa ccccatgagt
      721 agcggcgacc ctggctggag ggtatgccga ggtcctggcg caccttgtag tggagccact
      781 cggccaggcc ctcggccgac tggacgccga gcccatgcgt gtagagctgc tcggcgaact
      841 cgccgtcctt ctccagatgc tccatcagct cggtcacctc ggggccgacc gtcacgccct
      901 gcagggcgac cacgtcgcgg atcccctcgt cattcagggg ccggtagaag tcggtcaggc
      961 agatcctgtc gtgcttgggc tggcggggga agacgaggcg gttgatctcc ctgtcctgat
     1021 cctcggggtc gaagatgacc agctcgttgc cgtccgcgtt gcaggggaaa tagccgagac
     1081 gggccttcgg gtgaaggtag tcctgctcgc gccacatccg ctccagccgc ggccggaact
     1141 cctcgcttac gagcttgtcc cactcctcgc ccttgactcc cttgccgccc cagtggagct
     1201 tgaagagcac atgcaggtcg aggtggtcga agacctcgtc gaagtcgacg ggtacttcct
     1261 gtacgcccca ccacggcggg gtcgggatcg gcgcgtcggt gctcgcgctc gagcggaccg
     1321 aatcgtcatc gagcggcggc aggtcctcaa cgacgaccgg cttgttccgg taatcctcgg
     1381 cctcctgctt gaggcggccg atcagggctt cgcgggcccc gtcatcgatc agggcatcca
     1441 tcgtgtcgag cccctggaag gcgtccttgc agtagatgac tcccggctcg tagatctcgt
     1501 cggactcctt gccatgcggg tagagggtgc ggcggccgaa gtcgcggttg atcgcggcgc
     1561 cgccgatcag caccgggtat tcgagtccgc gctgatggag ttcctggacc gcgatcggca
     1621 tctgcttgga agtcgagacg agcagcgcgg agaggccgat cgcatcggct tcgtgttcga
     1681 tcgccgcatc gatgatcgtg tcgaccggga cctgcttgcc gaggtcgatc accgtgtagc
     1741 cgttgttggt gaggatggtg ttgaccagtg acttgccgat gtcatggacg tcaccgaaga
     1801 cggtcgcgat cacgaccttg cccttggtgt ggccctcgat ccggtcgagg tagttctcga
     1861 gcctggacac ggccttcttc atcacctcgg ccgactgcag cacgaagggc aggatcaact
     1921 cgccggcgcc gaacttgtcg ccgacctcct tcatcgccgg cagcaggacc tcgttgagtg
     1981 tggggacggc accgatcttc tctaccgact tgtcgatcca gtcctcgacg ccttccttct
     2041 tgcggcgcag gatcatgaag tggagcgcct cttccggctc catgcccgcc atcgcgtcct
     2101 cggcttcggc ctcgtcttcc tcgcccttcg actcgaagtg ggcgatgaaa cgctccagcg
     2161 cgtcctcgcg ccggttgaag accaggtcct cggtcagctc gatctcgtcg gccgggatct
     2221 cgccgtaggg cttgatgtga ttcgggttga ccatcgccag gtcgagcccg gcctccacgc
     2281 agtggtggag gaagaccgag ttgagaaccg cgcgggccgg ttggctgaca ccgaagctga
     2341 cgttggaaac gccgagcgag gtcttgacgc cggggatctc c
//
LOCUS       DAOTIP010000311         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52944_length_2380_cov_3.514839, whole genome shotgun sequence.
ACCESSION   DAOTIP010000311 DAOTIP010000000
VERSION     DAOTIP010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52944_length_2380_cov_3.514839"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="glyA"
                     /locus_tag="P5138_06540"
     CDS             <1..1099
                     /gene="glyA"
                     /locus_tag="P5138_06540"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRV60271.1"
                     /translation="NKYAEGYPGRRYYGGCEQVDIVEQLAIDRAMDLFGAGHANVQPH
                     SGAQANNAAYMALLEPGDTIMGLALDHGGHLSHGMKLNVSGKLYNVVPYHVSRDDLRV
                     DMDEVARLAEETRPNLIVAGWSAYPRQLDFAAFREIADSVGAKLMVDMAHFAGLVAAG
                     EHPNPVPHADVVTTTIHKTLGGPRSGMILSTEELKADVNRAVFPGQQGGPLMHVIAAK
                     AVALGIASTDVFAERQRRTVENAQALAAGLIDGGIDVLTDGTDVHLVLVDLTSTGLDG
                     QTGEDRLDQVGITVNRNAIPFDERPPMNPSGLRIGTPALSTRGFQPEDMTEIAQIIST
                     ALGPDFEVQRDSLAARSQALMDKYPLYPGLG"
     gene            1146..2153
                     /locus_tag="P5138_06545"
     CDS             1146..2153
                     /locus_tag="P5138_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HRV60272.1"
                     /translation="MKVWFDCTAAAHPVVLRPVIERFRERGDEILVTARENGETVGNL
                     ERLGIPHLVVGEHGGAGKFGKAWALASRSTRLARPVWKYRPDLAVAHGSVDLAVLSRS
                     FFIPSAQMQDYEFAGLQRKIAWKVARRVLVPDSIPVDRLVKAGAKEEKLVRFPGLKED
                     YYLADFEPDSSVLSAMGIDLQRILVIIRPPPETAAYHADNPLYEQVIDRLATAEGVTA
                     VVIPRTPAQGERVRGRNSPNLIVPDRAVDAQSLIAFADLVVSAGGTMNREAVALGTPV
                     YTTFAGKMGGVDEALITDGSLKLLESPGEFELVKRNDPTGPRNPRDPEILVEAMLGAL
                     G"
     gene            2196..>2380
                     /locus_tag="P5138_06550"
     CDS             2196..>2380
                     /locus_tag="P5138_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRV60273.1"
                     /translation="MVDGIDAVWAFLTAALITLLLVRPAERLARRVDAIDEPGPRSQH
                     TDPTPRMSGLAILAGIQV"
BASE COUNT          418 a          820 c          774 g          368 t
ORIGIN      
        1 caacaagtac gcggagggat accccggccg ccgctactac ggcggctgcg agcaggtcga
       61 catcgtcgaa caactggcga tcgaccgggc gatggacctg ttcggagcgg gacacgcaaa
      121 cgtccagccg cactcgggcg cccaggccaa caacgccgcc tacatggcgc tgctcgagcc
      181 cggcgacacg atcatgggcc tggccctcga ccacggcggg cacctgagcc acggaatgaa
      241 gctgaacgtt tccggcaagc tctacaacgt ggtgccgtac cacgtgtcga gagacgatct
      301 tcgggttgac atggacgagg tcgcaaggct tgcggaggaa acccggccga acctgatcgt
      361 ggcgggctgg tctgcctatc cccgtcagct cgatttcgcc gctttccggg aaatcgccga
      421 ctcggtcggt gcgaagctca tggtcgacat ggcacatttc gccggactgg tcgcggccgg
      481 cgagcatccg aaccctgtcc cgcatgccga cgtggtcacc accacgattc acaagacact
      541 gggcggaccg cggagcggca tgatcctcag taccgaagaa ctgaaggcgg acgtcaaccg
      601 cgcggtcttc cccggccagc agggtggtcc gctgatgcat gtgatcgcgg ccaaggcggt
      661 cgccctcgga atcgcttcca ccgacgtctt cgccgaacgt cagcggcgca ccgtcgagaa
      721 cgcgcaggcg ctcgcggccg gcctgatcga tggcggcatc gacgtcctca ccgacggcac
      781 cgacgttcat cttgtcctcg tcgacctgac ctcgaccggg ctcgacgggc agaccggcga
      841 ggatcgcctc gaccaggtcg ggatcaccgt caaccgcaac gcgattccct tcgacgagcg
      901 cccgccgatg aatccttccg gcctgaggat cggaacgccg gcgctctcga cccggggctt
      961 ccagccggaa gacatgaccg agatcgcgca gatcatcagc acggcgctcg gccccgactt
     1021 cgaggtccaa cgggattcgc tcgccgcccg cagccaggcg ctcatggaca agtaccccct
     1081 ctacccgggg ttgggctgat ccacagcatc cgtcaagggc ggccgtaccc gcggaggatc
     1141 cgaagatgaa ggtctggttc gactgcacgg ctgcggccca cccggtggtg ctgcggccgg
     1201 tgatcgagcg tttccgtgag cggggcgacg agatcctggt caccgcccgc gaaaacgggg
     1261 agacggtcgg taatctcgag cggctcggaa tcccgcatct ggtggtcggc gagcacggcg
     1321 gcgccggcaa gttcggcaag gcctgggccc tggccagtcg atcgacccgg ctggcgcgtc
     1381 cggtctggaa gtaccggccg gacctggcgg tggcacacgg ctcggtcgac ctcgccgtgc
     1441 tgtcccgctc cttcttcatc ccctcggccc agatgcagga ctacgaattc gccggactcc
     1501 agcggaagat cgcctggaag gtggccagac gcgtactggt cccggactcg atcccggtcg
     1561 accggctggt caaggccggc gcgaaggagg agaagctggt ccgctttccc gggctgaagg
     1621 aggactacta cctggctgac ttcgaaccgg acagttcggt gctctccgcg atgggcattg
     1681 accttcagcg gatcctggtc atcatccgcc cgccgcccga gaccgccgcc taccacgctg
     1741 acaatcccct ctacgagcag gtcatcgacc gcctcgccac ggcggaaggc gtgaccgcgg
     1801 tggtgattcc gcgaaccccg gcccaggggg aaagggtcag ggggaggaac tcgccgaacc
     1861 tgatcgtccc cgaccgggcg gttgacgccc agagcctgat cgccttcgcc gacctcgtgg
     1921 tcagcgcagg tggcaccatg aatcgcgagg cggtcgccct cggcaccccg gtctacacca
     1981 ccttcgccgg gaagatggga ggggtggacg aagccctgat caccgacggc agcctgaagc
     2041 tgctggaaag ccccggggaa ttcgaactcg tcaagcggaa cgaccccacc ggtccgagaa
     2101 acccccgcga cccggaaatc ctcgtcgaag cgatgctggg agctctcggc tgatcgaaac
     2161 gccgtccgtc ggacggtcgt gggaatcttt cttccatggt tgacgggatc gatgccgtct
     2221 gggccttcct cacggcggct ctgatcactc tccttctggt tcggcccgcc gagcgcctcg
     2281 cccggagggt ggatgcgatc gatgagcccg ggccccgcag ccagcacacc gacccgaccc
     2341 cccggatgtc cggcctggcg atcctcgccg gaatccaggt
//
LOCUS       DAOTIP010000312         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_52953_length_2380_cov_2.995269, whole genome shotgun sequence.
ACCESSION   DAOTIP010000312 DAOTIP010000000
VERSION     DAOTIP010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52953_length_2380_cov_2.995269"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..919)
                     /locus_tag="P5138_06555"
     CDS             complement(194..919)
                     /locus_tag="P5138_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV60274.1"
                     /translation="MALPPPPLPAAFLSLFGDAFNFVFSGREAETGGVTVGGTSQVIE
                     LATKQIEVTLLALAVAILLAMPVGLWLGHRQQGEFLAVSAGNAGRAIPELALIALMAA
                     AIGVGLVNLVVALAILGIPPILTNTYVGISQVDRASVEAARGMGMSETGIVRKVELPL
                     AVPTIMGGVRTAAINIVATATIAPLAGILTLGDFIIARNVYGDAGVLAGAILVALMAL
                     ALEGTLATIQWAMTSKGLKLNRS"
     gene            complement(919..1641)
                     /locus_tag="P5138_06560"
     CDS             complement(919..1641)
                     /locus_tag="P5138_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60275.1"
                     /translation="MIEQLAAVDQSFFTDRSSGNCIQDNKLFCPGWVVDNYDRYVDPT
                     LQHLVLVGSAVAGGFLIAFGLAILAHRHRFLQTPFLAGTGILYTIPSISFFFLLLPIT
                     GRGSLTAIIALTAFNLQIIFRNITTGLRNVPPNVTEAARGIGLTRSQILWQVEIPLAM
                     PEIIAGLRIATVSTVALATLAVFANGGGLGTEIVTGSNITFKTGIIVAGSIAVIMAIV
                     FDGVLLLVGKLLAPWKRKQAIS"
     gene            complement(1634..>2380)
                     /locus_tag="P5138_06565"
     CDS             complement(1634..>2380)
                     /locus_tag="P5138_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60276.1"
                     /translation="YPAQLSGGQQQRVGVARALAVDPPVMLMDEPFGAIDPINRSRLQ
                     NEFLRLQAELRKTVLFVTHDVDEAIKMGDRVVVLKKGGHLAQYATPTELLMEPADDFV
                     EDFVGADRALKRLALMRVRDVDLWTAPLAQAGDPSEPVRTAVAAAEVPYALLVDGENR
                     PQGWLSERDLAGEQVPATPDSSADPLIDADSVLRDGLATLLQSGSLYAPVVDARGRLT
                     GVLSVEIVSDFLASDEASESELSAAERPHD"
BASE COUNT          406 a          740 c          847 g          387 t
ORIGIN      
        1 ccgctgtcgc cggagtcgtc atcgtcggaa ccgcaggcga ccaggccgag ggccagcgcc
       61 atcagggcga ccatcagcag gtagcgcaat ttcttgacag gcatctgtcg tttctctcct
      121 tgactgggga ttgactggtg ggtctctctt ggaaaccgta tccgcccggg cgggcgttca
      181 agcagaaatc gcttcaggag cggttcagct tcagtccctt ggaggtcatc gcccactgga
      241 tcgtggccag ggttccttcc agggccagag ccatcagcgc aacgaggatt gccccggcga
      301 ggactcctgc gtccccgtag acgttgcggg cgatgatgaa gtcgcccagg gtcaggatgc
      361 cggccagcgg ggcgatggtc gccgtggcga cgatgttgat cgccgcggtc ctgaccccgc
      421 ccatgatggt cggcaccgcc aggggcagct cgaccttgcg gacgattccg gtttcggaca
      481 tgcccatgcc acgggcggcc tcgaccgagg cacggtccac ctggctgatc ccgacatagg
      541 tgttggtgag gatcggcggg atacccagga tcgcgagggc gaccaccagg ttgaccagcc
      601 cgaccccgat cgccgccgcc atcagggcga tcagggcgag ctcggggatg gcccggcccg
      661 cgttaccggc ggaaacggcc aggaactcac cctgctggcg gtgaccgagc cagagtccga
      721 ccggcatcgc caggaggatc gctaccgcca gggccagcag ggtgacctcg atctgcttgg
      781 tcgccagctc gatcacctgg ctggtgcccc cgaccgtgac cccgccggtt tcggcttccc
      841 ggccggagaa gacgaaattg aaggcgtccc cgaaaaggct cagaaatgcg gcgggaaggg
      901 gcgggggcgg cagggccatc aggagatcgc ctgcttccgc ttccaggggg cgagcagctt
      961 gccgaccaga aggaggactc cgtcgaagac gatggccatg atcaccgcga tcgatccggc
     1021 cacgatgatc ccggtcttga aggtgatgtt ggaaccggtg acgatctcgg tgccgagtcc
     1081 gccgccgttg gcgaagacgg ctagcgtcgc gagggcgacg gtgctgaccg tggcaattct
     1141 cagcccggcg atgatctcgg gcatcgccag gggtatttcg acctgccaga ggatctgtga
     1201 ccgggtcagg cctatgcccc gggctgcctc ggtcacgttg gggggaacgt ttctcaggcc
     1261 ggtggtgatg ttgcgaaaga tgatctggag gttgaaggcg gtcagggcga tgatcgcggt
     1321 gaggctgccg cggccggtga tcggcagcag caggaagaag aacgagatcg acgggatcgt
     1381 gtagaggatt ccggtgccgg ccaggaacgg cgtctggaga aaacggtgcc ggtgggcgag
     1441 gatggccagg ccgaaggcga tcaggaaccc gccggcgacc gcgctcccga ccaggaccag
     1501 gtgctgcagg gtcgggtcga cgtagcggtc gtagttgtcg acgacccatc cggggcagaa
     1561 gagcttgttg tcctggatgc agttgccgct ggagcggtcg gtgaaaaagc tctggtcgac
     1621 cgctgcgagc tgctcaatca tggggccgct cggctgcaga gagttcggat tcggaggctt
     1681 cgtccgaggc gaggaagtcg gagacgatct cgaccgagag aaccccggtc aaccttcccc
     1741 tggcatcgac caccggggcg tagagggatc cgctctggag cagggttgcg aggccgtccc
     1801 ggaggacgct gtcggcgtcg atcagggggt cggcggacga gtccggcgtc gccggcacct
     1861 gctcgccggc caggtcgcgc tcggagagcc acccctgcgg ccggttctcg ccgtcgacca
     1921 gcagcgcata cggcacctcg gctgcggcga ctgccgttcg caccggctcg gacggatccc
     1981 cggcctgggc gagtggagcg gtccagaggt cgacgtcccg gaccctcatc agggccagcc
     2041 gcttcagggc ccggtcggca ccgacgaagt cctcgacgaa atcgtcggcc ggttccatca
     2101 gaagttcggt cggggtcgcg tactgggcca ggtggccgcc cttcttcagc accaccaccc
     2161 ggtcgcccat cttgatcgcc tcatcgacgt catgggtgac gaagaggacg gtcttccgca
     2221 gttcggcctg aagcctgagg aattcgttct gcaggcgcga ccggttgatc ggatcgatcg
     2281 ccccgaaagg ctcgtccatc agcatcaccg gagggtcaac ggcgagcgcc cgtgccactc
     2341 cgacccgctg ctgctggccg ccggagagct gggccgggta
//
LOCUS       DAOTIP010000313         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_53079_length_2376_cov_2.912969, whole genome shotgun sequence.
ACCESSION   DAOTIP010000313 DAOTIP010000000
VERSION     DAOTIP010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53079_length_2376_cov_2.912969"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="P5138_06570"
     CDS             <1..454
                     /locus_tag="P5138_06570"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HRV60277.1"
                     /translation="IKTQDLFARPLTRGEKEQLYAESITWYARYGVSMRPVPEDFAAF
                     ERYWQRMFEEVLEPTRPAGEWVRRVRGIPAPPSWPELLWKPVGPVVGIAGPWLTKVTL
                     PESAREMFGCELTDRDRGALRMLQKSVRATWPLIPERFRMQPRARAGF"
     gene            complement(455..>2376)
                     /locus_tag="P5138_06575"
     CDS             complement(455..>2376)
                     /locus_tag="P5138_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60278.1"
                     /translation="HGDMDEAAYYSTALSQHRIFDRWKVGVAKDNPSLVAGNSPFNTE
                     GPATDPDSPKNGGLYAPGKTPHADFDCHDPDDLPGDSDIASCTATVDGNPILSGDPLP
                     DSPGVHTFVVTAIDEGGNTYVHTHTYTVKTFKDLFNTDGPVAYYRLGDGPGPVMADSS
                     GNGRNGEYKNAQDSGPLGISGDGDHARNFFGTSGYGFVNGIAAPRFQSTIEAWVKPLG
                     DPDPVSTDFVGGSNPAGWSVQNIWNPGGTAGVSDNVLYADGASFGTNATFGSGRTVNF
                     VANIPAEPYSHAGFGADYNNPPWAMFSVKGDGRLYARTSNGVDPAVETDLGTGYLGSN
                     HRYTIDWHADRLVYYVDGDPVAVHAVSFGPVQMRPLISNYSAGGASVAVDWLRLSPYA
                     NRNQSIVGHGDGGEIYIKDGYFVYRRMDTSVTSSVAVRPGEWQQVVGTWDGVDIRIYV
                     DGVEAGKVESTKRPSSVSTFYVGYGELAPWFNGSIDEVAYYPVALNTNRVFQHWLADP
                     PPDDLNAATNGVQPDDVSDIHPTPGAGGGTDPSGTSDEPKSDDPGSSDPGSGDPDNPT
                     SDDPSTDDPSGDPGEGADDGTVEPIYEKSDKPKAAANRSKAAKAKKRRAAVRKCKKIS
                     KKSKRKACLKRARSI"
BASE COUNT          397 a          747 c          797 g          435 t
ORIGIN      
        1 gatcaagacc caggacctct ttgcccgtcc cctgacccgg ggagagaaag aacagctcta
       61 cgccgaatcg atcacctggt acgcccgcta cggcgtgagc atgcggccgg tgccggagga
      121 cttcgcagcc ttcgagcgct actggcagcg catgttcgag gaagtcctcg agccgacccg
      181 ccctgccggc gagtgggttc ggagggtacg gggcattccc gccccaccaa gctggccgga
      241 acttctctgg aagccggtcg gcccggtggt cggcattgcc ggaccgtggc tgaccaaggt
      301 cacgttgccg gaatccgccc gggagatgtt cggctgcgaa ctcaccgacc gtgaccgcgg
      361 agcgttgcgg atgcttcaga aatccgtgag agcaacctgg ccgctgatcc cggagcgctt
      421 caggatgcag ccccgcgcca gggccggctt ctgatcagat cgagcgggcc cgcttcaggc
      481 aggccttacg cttcgacttc ttgctgatct tcttgcactt ccggaccgcg gcccggcgct
      541 tcttcgcctt ggcggccttc gacctgttcg cggccgcctt cggcttgtcg gacttctcgt
      601 agatcggctc aaccgtgccg tcgtcggcgc cctcaccggg gtcaccggac gggtcgtcgg
      661 tggacggatc gtcagaagtc gggttgtcgg gatcgccgct gccgggatcg ctgctgccgg
      721 ggtcgtcgga cttgggctcg tcggaagtgc ctgaaggatc ggttccgccg cccgctcccg
      781 gagtcgggtg aatgtcactc acgtcgtcag gctgcacacc gttggtcgca gcgttcagat
      841 catccggcgg cgggtcggcc agccagtgct ggaacacacg gttcgtgttc agggccaccg
      901 ggtagtaggc gacctcgtcg atcgaaccgt tgaaccaggg agccagttcg ccgtagccga
      961 cgtagaacgt cgaaacggag ctcggccgct tggttgactc gacctttccg gcctcgaccc
     1021 cgtccacgta aatacggatg tccacaccgt cccaggtgcc gaccacctgc tgccactcac
     1081 ccggccggac cgcgaccgaa gaggtgacgc tcgtgtccat gcggcggtat acgaagtagc
     1141 cgtccttgat gtagatctcg ccgccgtcac cgtggccgac gatcgactgg tttcggttcg
     1201 cgtaaggact caggcggagc cagtcgacag ccaccgacgc cccgccggcg ctgtagttcg
     1261 agataagcgg acgcatctgg accgggccga aactgaccgc atgaacggcg accgggtcac
     1321 cgtcgacgta gtagacaagg cggtcagcat gccagtcaat cgtgtaacga tgattggacc
     1381 cgaggtatcc ggtgccgagg tcggtctcga ccgccgggtc gactccgttg ctggtccgtg
     1441 cgtaaaggcg accgtctccc ttgacgctga acatcgccca gggcggattg ttgtagtcgg
     1501 caccaaagcc ggcgtgactg tacggctcgg cagggatgtt cgccacgaag ttgacggtcc
     1561 ggcccgagcc gaacgtagcg ttggtgccga agctggctcc gtcggcgtag aggacgttgt
     1621 ccgaaacgcc agccgtcccg ccggggttcc agatgttctg gaccgaccag ccggccgggt
     1681 tcgagcctcc gacgaaatca gtgctgaccg gatcgggatc acccagcggc ttgacccacg
     1741 cttcaatcgt cgactggaag cgcggagcgg cgatcccgtt cacgaagccg taaccgctgg
     1801 ttccgaagaa gttgcgggca tggtcaccgt cgccggaaat gccgagcgga cctgaatcct
     1861 gggcgttctt gtactcgccg ttcctgccgt tcccggacga atcggccatg accggtcccg
     1921 gaccgtcacc gaggcggtag taggcaaccg gaccatcggt gttgaacagg tccttgaagg
     1981 tcttgaccgt gtaggtatga gtgtggacgt aggtgttgcc gccctcgtcg atcgcggtga
     2041 cgacgaaggt gtggacaccc ggggaatccg gcagcgggtc gccactcagg atcgggttgc
     2101 cgtccaccgt cgcggtgcag gaagcgatgt cggaatcacc ggggaggtca tccgggtcat
     2161 ggcagtcgaa gtccgcatgc ggggtcttgc ccggggcgta gagaccgccg ttcttcggcg
     2221 aatcggggtc cgtggccggg ccctcggtgt tgaagggcga gttgccggca acaagcgaag
     2281 gattgtcctt ggcaacgccg accttccagc gatcgaagat ccgatgctgg ctgagtgcgg
     2341 tcgagtagta ggccgcctcg tccatgtcgc cgtgga
//
LOCUS       DAOTIP010000314         2374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_53134_length_2374_cov_2.504959, whole genome shotgun sequence.
ACCESSION   DAOTIP010000314 DAOTIP010000000
VERSION     DAOTIP010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53134_length_2374_cov_2.504959"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5138_06580"
     CDS             <1..518
                     /locus_tag="P5138_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60279.1"
                     /translation="KRVIAWARGEGQAFGIDPERIFLAGGSAGAHIAAMAGLTAGKPE
                     LQPGFEQVNTAVSGVIGIYGYYGGLESPGGRPPAVPSSPRRHVQPGAPPFLLIHGDHD
                     SVVGRGGAAALVKELQGAGNQASLAVLPGAEHTFDLLRSVRTENTIDSIVDFAAWAER
                     SENRDLSQSRR"
     gene            530..1288
                     /locus_tag="P5138_06585"
     CDS             530..1288
                     /locus_tag="P5138_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60280.1"
                     /translation="MGAKGAKGNLPGGLRTGLVGLGLLALAITCLLTSPAPDRSNAET
                     RANAEPQGGILPCGSHVVLTAVYRNRDGRIHFEGVADPKFAGRQARIYDRDNHLVAAT
                     TVGEDGIFWANANSKDEGYTWLTRFIARVGGSESRWKRIGQAVGLRDRQPFELEPTAR
                     NHGKTLERTRVRVKVAGGHSNLLVVGLQVGCSRFDVIELASTYTDSNGVSDLNLPRPP
                     ADRPYWIFRVSTEDDWKISPPIVVKSVPESGPTG"
     gene            1361..>2374
                     /locus_tag="P5138_06590"
     CDS             1361..>2374
                     /locus_tag="P5138_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRV60281.1"
                     /translation="MTQEQLEAYHEYTRKHGVNRGLYLFARIVLQPFFLIYLRLSRQG
                     RGNAQIKGATIVAANHRSFLDPFVIGTCLPWNKPMNYVAKVELFEKRWQGFLLCRVGA
                     FPIRRGEADELAIETAELVLARGGTICIFPEGTRIRKGSLGKPKRGVGRLALRSGAPV
                     LPVAVTGSENVRRGWQIRPRKVDIRVGRPMSFPQSESPSPALAASVTDRIWPNVELQW
                     EDIGGLPPMRRAAVIGAGSWGTAVAVLLARGGLEVQLGTRTADQAEEMALARENTRYL
                     PGVRLPDGIEVRASSQIELGGVDLLCIAVPSASYPQAVGAVADRVGRRAGVLLLAKGL
                     IAPK"
BASE COUNT          387 a          793 c          802 g          392 t
ORIGIN      
        1 tcaaacgcgt gatcgcctgg gcccgcgggg aggggcaggc attcggcata gatccggagc
       61 ggatctttct ggctggcggc tcggccgggg ctcacatcgc ggcgatggcc gggctgaccg
      121 cgggcaagcc ggagctgcaa ccgggattcg agcaggtgaa cacggccgtc tccggggtga
      181 tcgggatcta cggctactac ggcggccttg aatcacccgg cggccgcccc ccggctgtcc
      241 cctccagccc ccggcgccac gtgcagccgg gcgcaccccc gtttctgctc atccacggag
      301 accacgacag cgtggtcggg cggggcggag ccgcggccct cgtcaaggaa ctccagggcg
      361 ccggcaacca ggcgtcactg gccgtcctgc ccggggccga acacaccttc gacctgctgc
      421 gctcggttcg caccgaaaac acgattgact cgatcgtcga ttttgccgcc tgggcagaac
      481 gttcggaaaa tcgtgatctt tcacaatccc ggcgctaaga tgggctgttg tgggggcgaa
      541 gggagcaaag gggaatcttc ccggcgggct gcggaccggc ctggtcggtc tggggctgct
      601 tgcgctcgcg attacctgtc tcctgacttc gcctgcgccc gatagatcga acgccgagac
      661 tcgggccaac gctgagccgc aagggggcat ccttccctgc ggcagccacg tggtccttac
      721 cgcggtctat cgcaacaggg acgggcggat ccatttcgaa ggggtcgccg atccgaaatt
      781 cgccggcagg caggcccgga tctatgaccg ggacaaccat ctggtcgcag cgacgaccgt
      841 gggcgaggac ggaatcttct gggcgaacgc caattcaaag gacgagggtt acacctggct
      901 cacccggttc atcgccaggg tgggtggcag cgagtcccgc tggaaacgga tcggccaggc
      961 ggtcggcctc agggaccggc agccctttga actggagccg acggcacgca accacggcaa
     1021 gaccctggag cgcaccaggg tcagggtcaa ggtggccggc ggccacagca atctcctggt
     1081 ggtcggtctg caggtcggct gttcccgatt cgacgtgatc gaactcgctt cgacctacac
     1141 ggactcgaac ggggtgtccg acctgaacct gccccgtccc ccggcggatc gtccctactg
     1201 gatcttccga gtcagcaccg aagacgactg gaagatctcg cccccgatcg tcgtcaaatc
     1261 ggtgccggaa tccggcccga ccggctgatt cgcccgggcc cgcccgaaaa cggcgaggac
     1321 ccgttgtaac cgggtccggg ggtttgtgta ggactctttt gtgactcagg agcaactcga
     1381 ggcctaccac gagtacacgc gcaagcacgg ggtgaaccgc ggtctttacc tcttcgcgcg
     1441 catcgttctg cagccgtttt tcctaatcta cctgcggctt tcgcgtcagg gcaggggtaa
     1501 cgcgcagatc aagggcgcga ccatcgtcgc cgccaatcac cgcagcttcc tcgacccgtt
     1561 cgtgatcggc acctgcctgc cctggaacaa gccgatgaat tacgtggcga aggtggaact
     1621 gttcgagaag cgctggcagg ggttcctcct ctgccgggtc ggggccttcc cgattcgccg
     1681 gggagaggct gacgagctgg cgatcgaaac cgccgagctg gttctggccc ggggcgggac
     1741 gatctgcatc tttcccgaag gcacccggat caggaagggt tcgctcggca agccgaagcg
     1801 gggcgtcggg cgtctcgctc tccggagcgg ggcaccggtt ctgccggtcg ccgtcaccgg
     1861 ttccgagaac gtgcggcgcg gctggcagat ccggccgcgc aaggtcgaca tccgggtcgg
     1921 ccggccgatg tcctttcccc agtcggaatc gccttctccg gccctcgccg catcggtcac
     1981 cgatcgcatc tggccgaacg tcgaacttca atgggaagac atcggcggtc tgccgccgat
     2041 gcgccgggcc gcggtcatcg gagccggcag ctggggcacc gcggtcgcgg tgctgctggc
     2101 ccgcggcggg ctcgaagtcc agctcggcac ccgcaccgcc gaccaggcgg aggagatggc
     2161 cctcgcgcgc gagaacacca ggtatctgcc cggcgttcgc ctgcccgacg gcatcgaagt
     2221 ccgggcctcc agccagatcg aactcggtgg agtcgacctg ctctgcatcg cggtcccgtc
     2281 agcttcctac ccgcaggcgg tcggagcggt cgccgaccgg gtcggtcgcc gggccggggt
     2341 actgcttctc gccaaggggt tgatagcccc caag
//
LOCUS       DAOTIP010000315         2368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_53310_length_2368_cov_2.734976, whole genome shotgun sequence.
ACCESSION   DAOTIP010000315 DAOTIP010000000
VERSION     DAOTIP010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53310_length_2368_cov_2.734976"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="P5138_06595"
     CDS             <1..509
                     /locus_tag="P5138_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRV60282.1"
                     /translation="SVSLCALFGVSALYHRINWKSMRSRMMMRRLDHSMIFLLIAGTV
                     TPFALLNLTGTWSTAILIAVWSAAIVGIIVELLWISSPKWVSAAMYVAVGWIGAVAFP
                     QIVGTAGVWAGLLIATGGILYTVGAVVYATGRPDPSPAYFGYHEVFHLFVIGAAAFHF
                     AAVAMFAL"
     gene            509..1375
                     /locus_tag="P5138_06600"
     CDS             509..1375
                     /locus_tag="P5138_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60283.1"
                     /translation="MSHTDVTSGLEGAPEWSSIAVGDHWIRTFQAGPHQASEAVVFIH
                     GNPGSAADFEGLVKAVAPPRRAVTFDLPDFGQSIAAPGFTHTQSEYAEFIGDAFKALG
                     IRRAHLVLHDLGGPIGLEWASTHTGQIASITLINTGVLAGYRWHRTARIWQTPVAGEL
                     FQAAAFRSMFRRVINSQEPRGLPRDFIDGMYDNYDRRTRKAVLGIYRDLKKVGKTSKQ
                     LIPLLAAADLPALVIWGAGDPYLPVSFADRQRSSFPSARVHILPDSGHWPFIDDPPAV
                     TDLLIEFLATVE"
     gene            1467..1727
                     /locus_tag="P5138_06605"
     CDS             1467..1727
                     /locus_tag="P5138_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRV60284.1"
                     /translation="MGSLNIKTPEAHRLAKELAELTGESMAVATTEALRERRDRILRG
                     RGARADRFRKLAREIGQQLPPEMEQGDPTEFLYDENGLPLGN"
     gene            1750..2112
                     /locus_tag="P5138_06610"
     CDS             1750..2112
                     /locus_tag="P5138_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV60285.1"
                     /translation="MRSEPDAEDFIASIDGQADCRMSAGTYVELGVVIDSGSDPLGSV
                     KLDLMLGTEDIRIEPFTGNQARIARTAYKSFGKGSGHPARLNFGDCFSYALAMELGHP
                     LLFKGGDFAQTDVVPALA"
BASE COUNT          403 a          790 c          726 g          449 t
ORIGIN      
        1 actcggtttc cctttgcgcc ctcttcggag tcagtgcgct ctatcaccgg atcaactgga
       61 agtccatgag gtcaaggatg atgatgcgca ggctcgacca ttcgatgatc ttcctgctga
      121 tcgccggcac cgtcacgccc tttgccctgc tcaacctgac cggcacctgg tcgaccgcga
      181 tcctgatcgc ggtctggtcg gcagcgatcg tcgggatcat cgtcgagctg ctctggatca
      241 gttcgccgaa gtgggtcagc gccgcgatgt acgtggcagt tggctggatc ggggcggtcg
      301 ccttccccca gatcgtcggc accgctggcg tgtgggccgg gctgctgatt gccaccggcg
      361 gaatcctcta cacggtcggt gccgtggtct atgcgaccgg ccgtccggat ccaagtccgg
      421 cctacttcgg ctaccacgag gtcttccacc tcttcgtgat cggcgccgcc gccttccatt
      481 tcgcggccgt cgccatgttc gccctctgat gagccacacg gacgtgacct caggactgga
      541 gggcgcgccc gagtggtcaa gcattgcggt cggagaccac tggatccgca cctttcaggc
      601 cggccctcat caggcctcag aggcggtcgt attcatacac ggcaatcccg gctcggccgc
      661 cgacttcgag ggcctggtca aggccgtggc acctccgcgc cgggcggtca ccttcgacct
      721 gcccgacttc ggccagtcga ttgcggcgcc cggcttcacc cacacccaga gtgaatacgc
      781 ggagttcatc ggtgacgcct tcaaggccct gggcattcgc cgcgcccacc tggtcctgca
      841 tgacctcggc gggccgatcg gcctcgagtg ggcatcgacg cacaccggcc agattgccag
      901 catcaccctg atcaacaccg gtgtcctggc cggataccgc tggcatcgca ccgcccgaat
      961 ctggcagacc ccggtggccg gagaactgtt ccaggcagcc gctttccggt cgatgttccg
     1021 gcgggtcatc aacagccagg aacccagagg gctgccccgt gacttcatcg acgggatgta
     1081 cgacaactac gaccgccgga cccgcaaggc cgtgctcggg atctaccgag acttgaagaa
     1141 ggtcggcaag acctcgaagc agctgattcc gctgctcgcc gccgccgacc tgcccgctct
     1201 cgtcatctgg ggcgccggcg acccctacct gccggtctcc tttgccgacc gccagcggag
     1261 ctccttcccc tcggcccgcg tccacatcct tcccgactca ggccactggc ccttcatcga
     1321 cgacccgccc gcggtcaccg atctcctgat cgagttcctg gcgacggtcg agtagcgggc
     1381 gactggcggt cgcggacccg tcgccccagt ctggtggcgg aaaccggcga tggtagaacg
     1441 agagggtccg atggtagaat cgacccatgg gttccctcaa catcaagacc cccgaggccc
     1501 atcgactggc caaggagttg gcggaattga ccggcgaaag catggccgtc gcgaccaccg
     1561 aagccctgcg cgaacggcgg gaccggattc tgcgcgggcg cggggcgcga gcggatcgat
     1621 tccgcaagct cgccagggag attggtcaac agttgccgcc ggaaatggag cagggcgatc
     1681 cgacggaatt tctctacgac gagaacggat tgcctcttgg taattgacac ttctgcaatc
     1741 gtcgcggtga tgcggtccga gccggatgcc gaagacttca tcgcgtcaat cgacggtcag
     1801 gcagattgcc gaatgtcagc cggcacatac gtcgaattgg gggtggtcat cgattcgggg
     1861 tccgacccgc tcggatccgt gaagctggat ctcatgcttg gaaccgaaga catccggatt
     1921 gagccattta cggggaatca ggcgaggatc gcccgaaccg cctataagtc atttggcaaa
     1981 ggcagcggcc acccggcccg gctcaacttc ggcgattgct tctcgtatgc cctggccatg
     2041 gaactcggtc acccactgct tttcaagggc ggcgatttcg cgcagaccga tgtggttccg
     2101 gccctggcct gacgccgcaa ctcttggcct cctggcgatt tagttgacgc gcatcacctt
     2161 ttggggaata atgggaccaa gggggaatgt cacattgagg agagggtctt gaatttccgt
     2221 cgcatcgtcg ccgtctgttc gctttcggtc accttggctg gcgcggtagt cctggccggt
     2281 cccgcttcgg cgtcggatac ctccgaatac gaccaggccc gcgcctatgc cctcgaactg
     2341 gccgaaaaat gcgcgcccag cgccatgc
//
LOCUS       DAOTIP010000316         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_53972_length_2346_cov_3.408119, whole genome shotgun sequence.
ACCESSION   DAOTIP010000316 DAOTIP010000000
VERSION     DAOTIP010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53972_length_2346_cov_3.408119"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="P5138_06615"
     CDS             <1..406
                     /locus_tag="P5138_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60286.1"
                     /translation="ARLVRDVSAADELPARLAREREGLPRSAVIWLALLGAAFSMLPG
                     ITSIVSFGSLTFLVVFGLINLMHARHTAKPGWDRTVAYLAGAACFAASTGLIYYLARY
                     DRAGLALIAVCALSVMIARTVFLRSKQATVRE"
     gene            535..605
                     /locus_tag="P5138_06620"
     tRNA            535..605
                     /locus_tag="P5138_06620"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Cys,seq:gca)
     gene            complement(612..1013)
                     /locus_tag="P5138_06625"
     CDS             complement(612..1013)
                     /locus_tag="P5138_06625"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRV60287.1"
                     /translation="MKLIVNVDGGARGNPGPAAIGIVIRDGEGNVLQDLAETIGQASN
                     NVAEYQALIRGVEMAIELGGDELDIRGDSELVVKQMLGQYKVKHPDMKPLHTRAKELL
                     GGVGSWSISHVRREQNAEADALVNQALDAAS"
     gene            complement(1066..2004)
                     /locus_tag="P5138_06630"
     CDS             complement(1066..2004)
                     /locus_tag="P5138_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRV60288.1"
                     /translation="MSALPSTSASPPTSGGCPGWPEALTLFQRDLTAAGTAPRSMAAY
                     GRDLEQLRVWAVGRGLEPDQVGHKDLRRYAATLSAAGRAPATVARKLAAIRSFYGFLL
                     RTGRVGQNPADLVSAPRRKAKLPQVLTSEQMAGMLDAVPAETPLDLRDRAMFELAYSC
                     GLRSEEVLTLTVDSIDFDDELIRVLGKGSKHRLVPVGEPARLAVETYLTRSRPCLVVD
                     PAERTLFLSRNGRPLSASDLTRRLAIRMREAAAAGGVSPHALRHSFATHLLEGGADLR
                     TIQELLGHASISTTQVYTHLDAARLRDAYAGSHPRA"
     gene            complement(1976..>2346)
                     /locus_tag="P5138_06635"
     CDS             complement(1976..>2346)
                     /locus_tag="P5138_06635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRV60289.1"
                     /translation="TYTLTLWKNLNKVKSYTVAVGQQGLETPAGTYSIQDKQVDPVWT
                     VPNSAWAGDMAGEVVPGGTAENPLKARWMGIFDGAGIHGTDDTGSLGSAASHGCVRMS
                     IPDVIDLYDRVDVGTPIYIG"
BASE COUNT          361 a          779 c          788 g          418 t
ORIGIN      
        1 cgcccgtctg gtccgtgatg tgagcgccgc cgatgagctt ccggcccgac tggcccggga
       61 gcgggaaggc ctgcccagaa gcgcggtgat ctggctggcg ttgctcgggg ccgctttctc
      121 gatgctgccc ggcatcacct cgatcgtctc cttcggctcc ctgaccttcc tggtcgtatt
      181 cgggctgatc aacctgatgc atgcgcgcca cacggcgaag cccggatggg atcggacagt
      241 cgcctacctc gccggggcag cctgcttcgc ggcaagcacc ggattgatct attacctcgc
      301 ccgctacgac cgggccggtc tcgcgttgat tgcggtttgc gccctgtcgg tgatgatcgc
      361 ccgaacggtg ttcctgcggt cgaagcaggc gacggtccgg gagtgaaggc ccacctgcag
      421 actgctagat ttcaccttcg cggcgatcgg gattcttctg ctgaagccag gcagaaggtc
      481 gaacgttcga ctccctggcc gctacgggac ccacccctcg gggtcgtccc atttggcgcc
      541 ttggcggagt ggctacgcag cggcctgcaa agccgtttac accggttcga ctccggtagg
      601 cgccttgccg ctcaggaggc ggcgtcgagg gcctgattga ccagggcgtc tgcttcggcg
      661 ttctgttcgc gcctcacatg gctgatcgac cacgagccga cgccgcccag aagttccttg
      721 gccctggtgt gaagcggttt catgtcggga tgcttgacct tgtactggcc cagcatctgc
      781 ttgaccacca gttccgagtc accccggata tccagctcgt ctccgccgag ttcgatcgcc
      841 atctcgaccc cgcggatcag ggcctggtac tcggccacgt tgttggatgc ctggccgatc
      901 gtctcggcaa ggtcctggag cacgttgcct tcaccgtcgc ggatcacgat gccgattgcg
      961 gccggacccg ggttgccccg tgcgcccccg tcgacgttga cgatcaattt catggacgca
     1021 aatctatccg cccggaggaa gccgctcctt cccggcgggc agccttcagg cacgggggtg
     1081 gctgccggcg tacgcgtccc ggagccgggc agcatcgaga tgcgtataga cctgcgtggt
     1141 cgaaatcgaa gcgtgaccga gcagttcctg gatcgttcgc aggtccgctc ccccctccag
     1201 cagatgcgtg gcgaaggagt gccgcagggc atgcggcgat acgccgccgg ctgcggcggc
     1261 ctcgcgcatc cggattgcca gccggcgggt caggtccgaa gccgagagcg gacggccgtt
     1321 gcgggagagg aacagggtcc tttcggccgg gtccaccacg aggcagggcc tggagcgggt
     1381 gagataagtc tcgaccgcaa gccgggccgg ctctccgacc ggcaccagcc ggtgcttgga
     1441 gcccttgccg aggaccctga tcagctcatc gtcgaaatcg atcgaatcga cggtcagggt
     1501 caggacttcc tcgctgcgga ggccacagga ataggcgagc tcgaacatgg ccctgtcacg
     1561 aagatcgagt ggtgtctccg ccggcacggc atcgagcatg cctgccatct gctcggaggt
     1621 gagcacctgc ggcagtttcg ccttccgccg gggggccgag accaggtcgg cggggttctg
     1681 gccgacccga ccggtcctga gcaggaagcc atagaaactc ctgatcgcgg cgagcttgcg
     1741 ggccacggtc gccggcgccc gccccgcagc cgaaagcgtg gcggcgtaac gacgcaggtc
     1801 cttgtggccg acctgatcgg gttccagccc gcggccgacg gcccagaccc gaagctgctc
     1861 gaggtcacgc ccgtaggcgg ccatgctcct cggggcggtt ccggccgccg tcaggtcgcg
     1921 ctggaaaagc gtcagggcct ccggccagcc gggacagccg cccgacgtcg gcgggctagc
     1981 cgatgtagat gggagtgccg acatcgaccc ggtcgtagag gtcgatcaca tccgggatcg
     2041 acatccgcac gcagccatgg gaagcagccg aacccagtga tccggtgtcg tcagtgccgt
     2101 ggatgcccgc accgtcgaag atgcccatcc agcgggcctt gagcggattc tccgcggtgc
     2161 cgccggggac cacttcaccg gccatgtccc ccgcccaggc cgagttcggc acggtccaga
     2221 ccggatcaac ctgcttgtcc tggatcgagt aggtgccggc cggggtttcg aggccctgct
     2281 ggccgaccgc gacggtgtaa ctcttgactt tgttcaggtt cttccagagg gtcagcgtgt
     2341 aggtgc
//
LOCUS       DAOTIP010000317         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54125_length_2341_cov_3.238408, whole genome shotgun sequence.
ACCESSION   DAOTIP010000317 DAOTIP010000000
VERSION     DAOTIP010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54125_length_2341_cov_3.238408"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="P5138_06640"
     CDS             <1..236
                     /locus_tag="P5138_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60290.1"
                     /translation="LLGRDRDAATWLERSVDTCEEMGARPYLAHSRMHLALALRRIGN
                     PEPGRAEELMESGREIAEELGMAMLLDRIANWT"
     gene            complement(342..1052)
                     /locus_tag="P5138_06645"
     CDS             complement(342..1052)
                     /locus_tag="P5138_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRV60291.1"
                     /translation="MSLTTYEVDDRGVALIQLDRPKAMNAINTGMLVELIEHVAVARG
                     DENVRVLVISSTDYMALSAGADIKEDIDDDAKVNKMQLFADLYDAVVQFPKPTIAACH
                     GYVVGGGAEIAVACDIRIGGSNLQMRFPGAALGVPVGPARLVTLCGLSHAKYLLLSSN
                     TVKIDEALRMGLVNQAAPGAATENTALELAGRIAALDPEGVARLKRMLHEWDDIEGRS
                     EAEGRGQVEWQRSGPGLY"
     gene            complement(1069..1629)
                     /gene="pth"
                     /locus_tag="P5138_06650"
     CDS             complement(1069..1629)
                     /gene="pth"
                     /locus_tag="P5138_06650"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRV60292.1"
                     /translation="MLIVGLGNPGREYERSRHNAGFMVAALLAERWDMPRAKEKYNAR
                     ITEGRIRPGGPRVVLMTPLTYMNESGKSAGPARGSKKIPLEKVIAVYDEIDLPFGEIR
                     SRLGGGAAGHNGVKSLKQGFGSADFWRVRIGVDRPDSTDPDRVAGYVLGKFSESREQV
                     EGLIDDAARETERLVERIAAGETGEN"
     gene            complement(1681..>2341)
                     /locus_tag="P5138_06655"
     CDS             complement(1681..>2341)
                     /locus_tag="P5138_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRV60293.1"
                     /translation="ATTRTEFGTRISKRLRRDGKVPGVVYSDGKDAISFQVESRDARV
                     ILGEGHALFDLHIEGSDTVPVVVKEQQHHPVRGDLQHLDLQQVDLNQAIQAEVLIELT
                     GDDVSPGVKQGGVLEHVTREVTVEALPTDIPDSISLDVSEMEINDTLTLEQLVAPEGV
                     TLIADDPAEVTLVTLSPPRVEEEPEEDLEAEPEVVGEGEEGEEAAEGEGDSGEESSDE
                     E"
BASE COUNT          346 a          794 c          763 g          438 t
ORIGIN      
        1 tgctgctggg ccgcgaccgc gacgcggcga cctggctcga gcgatcggtg gatacctgtg
       61 aagagatggg cgcccggccc tacctggccc actcccgaat gcacctcgcc ctcgccctcc
      121 ggagaatcgg aaatcccgaa cccggccggg ccgaggagtt gatggagtca gggcgggaaa
      181 tcgccgaaga actcgggatg gcgatgctcc tcgacagaat cgccaattgg acatgagggt
      241 cctctgaccc gggaccggcc gccatcagga ccaggtccgg tcccgtccct cacccgaact
      301 accgccgtca ggaccaggtc cggtcccgtc cctcacccga actaatagag gccgggaccg
      361 gacctttgcc actcgacctg gccgcggcct tcggcttcgg agcggccttc gatgtcgtcc
      421 cattcgtgaa gcatccgctt gaggcgggcc acgccttcgg ggtccagggc ggcgatccgg
      481 ccggccagct ccagggcggt gttctcggtc gccgcaccgg gggcggcctg gttgaccaga
      541 cccatgcgta gcgcctcgtc aatcttcacc gtgttcgagg agagcagcag gtacttggcg
      601 tggctcagcc cgcagagggt gaccagccgg gccgggccga ccggaactcc gagggccgcg
      661 ccggggaagc gcatctggag gttcgagccg ccgatccgga tgtcgcaggc aaccgcgatc
      721 tcggccccgc ccccgaccac gtatccgtgg caggcggcga tggtcggctt ggggaactgg
      781 accacggcgt catagaggtc ggcgaagagc tgcatcttgt tcaccttggc gtcgtcgtcg
      841 atgtcctcct tgatgtcggc gccggccgag agggccatgt aatcggtcga ggagatgacc
      901 agcaccctca cgttctcgtc ccccctcgcg acggccacgt gctcgatcag ttcgaccagc
      961 atcccggtgt tgatcgcatt catcgccttg gggcggtcaa gctggatcag ggcgaccccg
     1021 cggtcatcga cttcataggt ggtcaggctc atggctcctc gtattccttc agttctcgcc
     1081 ggtttccccg gcggcgattc tctcgacaag tctttccgtt tcccgggccg cgtcgtcgat
     1141 caggccctcg acctgttcgc gactttccga gaacttcccc agcacgtagc cggcgacccg
     1201 gtccgggtcg gtcgaatcgg gccggtcaac gcctatccgt acccgccaga agtcggccga
     1261 tccgaaaccc tgcttgaggc tcttcacccc gttgtggccg gccgctccgc ctcccaggcg
     1321 actgcgaatc tcgccgaagg gcaggtcgat ctcgtcgtag acggcgatca ccttctccag
     1381 cggaatcttc ttcgagccac gggccggtcc ggccgacttg ccggactcgt tcatgtaggt
     1441 gagcggggtc atcaggacca ctcgtggccc gccgggcctg atccggcctt cggtgatccg
     1501 ggcgttgtac ttctccttcg ccctgggcat gtcccagcgc tcggcgagca gcgccgcgac
     1561 catgaagccg gcgttgtgcc ggctccgctc gtattcccgg cccgggttgc cgagaccgac
     1621 gatgaggatc gggccatcgg acccgtcctc cgggtctgag cgacgcagga aagccaggac
     1681 ctactcctcg tctgaggact cttcgcccga gtcgccttcg ccctcggctg cctcttcgcc
     1741 ctcttcgcct tcgccgacca cttcgggttc ggcttcgagg tcctcttccg gctcctcctc
     1801 gacgcggggc ggcgagagcg tgaccagggt cacttcggcg ggatcgtcgg cgatcagggt
     1861 gacgccttcc ggagcgacga gttgctcgag ggtgagcgtg tcgttgattt ccatctcgga
     1921 cacgtcgagc gagatcgagt cgggaatgtc agtcggcagc gcctcgacgg tgacttcgcg
     1981 ggtgacatgc tcgagcacgc cgccctgctt gacgccgggg ctgacgtcat caccggtcag
     2041 ttcgatcagg acttccgcct ggatcgcctg gttgaggtcg acctgctgca ggtcgagatg
     2101 ctggaggtcg ccgcgcaccg ggtggtgctg ctgttccttg accacgaccg gaaccgtgtc
     2161 ggaaccctcg atgtggagat cgaacagcgc atggccctcg ccgaggatga cccgggcgtc
     2221 ccggctttcg acctggaagg aaatcgcgtc cttcccgtcc gagtagacga cccccgggac
     2281 cttgccgtcg cgacggaggc gtttggagat tctggtgccg aattcggtgc gggttgtggc
     2341 g
//
LOCUS       DAOTIP010000318         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54209_length_2338_cov_2.773982, whole genome shotgun sequence.
ACCESSION   DAOTIP010000318 DAOTIP010000000
VERSION     DAOTIP010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54209_length_2338_cov_2.773982"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="P5138_06660"
     CDS             <1..545
                     /locus_tag="P5138_06660"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRV60294.1"
                     /translation="ATISLILIPAIALIVRVAMGNALSDFGGVIVPFDNEWWRLITAP
                     FAYLSVGYLFAVGLAIALFGPGLERRFGTLPAFLLLVACGALGTLAGSAVADQRDVVT
                     VIAGGNGIALGAIGAWFMVARSDARASGEAFEVAGVVVSAAVILLLPVVVPTADFWSG
                     LAGGLVGLLGGFTATRFGGN"
     gene            612..968
                     /locus_tag="P5138_06665"
     CDS             612..968
                     /locus_tag="P5138_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60295.1"
                     /translation="MSEETISDEELAAAVERLSDPERFREAEQLVSRVAPGLPSVLVA
                     ALGSGGWFGESHQSETLKAATIPDEDQRLTAIRALLSEETQMGMMVGVAVGWALRQEL
                     ENGGAIGAPKSENEES"
     gene            971..1681
                     /locus_tag="P5138_06670"
     CDS             971..1681
                     /locus_tag="P5138_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRV60296.1"
                     /translation="MEIRYHGHSCFQLNEGDLSLIIDPFLAPNNPKADVTADDITVTH
                     LALTHGHADHVADAVPVATKNKPQCVAMVEVANWLEMQGVENVSDPNLGGTVRFDGLS
                     IKLVPAWHTNTLPGSDERPFSAELGTPIGTPSGLIIEMGGLTIYDAGDTCLFGDMELI
                     GKRHAVDVALLPIGGHYTMDREDAAYAAELIGAKRVIPIHYDTFPVIETDAQAFADDL
                     SGRGIEGLILQPGESAEL"
     gene            complement(1742..>2338)
                     /locus_tag="P5138_06675"
     CDS             complement(1742..>2338)
                     /locus_tag="P5138_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60297.1"
                     /translation="RSGDDAGAVEVKDQVSTSPVDSSSATTTKTIADANPAKPVHVAT
                     PTNRSAALPAEPELEKASAEAPVTTPVDTATTDPSSTDTTPAPPAVDQATGEAAMPTE
                     PVTPAKPATSEGGDGLEGHTTYPPDEIVNESPATAPSETTTDPGTVGQVPGSAPTTPV
                     TSDPSTPVVPTSPTPAPAPTTPSTSQAAPADAAPVTGQ"
BASE COUNT          336 a          748 c          831 g          423 t
ORIGIN      
        1 gggcgacgat ctcgctgatc ctgatcccgg cgatcgccct gatcgtcagg gtggccatgg
       61 gcaatgcgct ctccgacttc ggcggcgtga tcgttccctt cgacaacgaa tggtggcggc
      121 tcataaccgc cccgttcgcc tatctgagcg tcggttacct cttcgcggtc ggtctcgcga
      181 tagccctttt cgggccgggg ctcgaacgcc gtttcggaac cctgcccgcc ttcctgctgc
      241 tggtcgcctg cggcgccctc ggcacgctcg ccggctccgc cgtagccgac cagcgcgatg
      301 tggtcacggt gatcgccggc ggcaacggca tcgccctggg cgccattgga gcctggttca
      361 tggtcgcccg ctccgatgcc cgggccagcg gggaggcctt cgaggtggcc ggggtcgtgg
      421 tctcggcagc ggtgatcctg ctgctgcccg ttgtggtccc gacggctgac ttctggagcg
      481 gtctcgccgg gggcctggtc gggctgctcg gcggattcac ggcgacccgc ttcggcggca
      541 actgaccgcc gcccggtctt tggtggacgc accggttcag cctgagggtc ctcgttggct
      601 aggcttgccc ggtgtccgag gagacgatct ctgatgagga acttgccgct gcggtcgagc
      661 ggctgagcga ccccgagcgg tttcgcgagg cggagcagct cgtctcgagg gttgccccgg
      721 ggttgccgtc ggtgctggtc gcggctctcg gaagtggtgg ctggttcggc gagtcgcatc
      781 agtcggagac cctgaaggcg gccacgatcc ccgatgagga ccagcgcctg accgcgatca
      841 gggcactgct ttcggaggag acccagatgg ggatgatggt cggagtcgcg gtcggctggg
      901 cactgaggca ggaacttgaa aacgggggcg ccatcggtgc tccgaaaagc gagaacgagg
      961 agagctgaag atggaaatcc gctaccacgg tcattcgtgc ttccagctga acgaaggcga
     1021 cctgtcgctg atcatcgatc ccttcctggc cccgaacaac ccgaaggcgg atgtcacggc
     1081 cgacgacatc accgtgaccc atctggccct gacccacggt cacgccgacc acgtagcgga
     1141 cgcggtcccc gtggcgacga aaaacaagcc acagtgcgtc gcgatggtgg aggtcgccaa
     1201 ctggctcgag atgcagggcg tcgagaacgt ctccgacccc aacctcggcg gcaccgtcag
     1261 gtttgacgga ctctcgatca agctggtgcc ggcctggcac accaacaccc tgcccggttc
     1321 agacgaacgt ccgttcagtg ccgagctcgg gacaccgatc ggcacccctt cgggactgat
     1381 catcgagatg ggcgggctga cgatctacga cgcgggcgac acctgcctgt tcggggacat
     1441 ggaactgatc ggcaagcgtc acgcggtgga tgttgccctg ctcccgatcg gtggccacta
     1501 cacgatggac cgggaggatg ccgcctatgc cgccgagctg atcggcgcga agcgggtcat
     1561 ccctatccac tacgacactt tcccggtgat cgagacggac gcccaggcct tcgctgatga
     1621 cctctcgggc aggggaatcg agggactcat cctgcagccc ggagagtcgg ccgaactcta
     1681 gacccggaaa cgacgaacgc cgcccgacgg gcggcgttcg cggttcacac ttgaggttct
     1741 cttactggcc ggtaaccggt gccgcatccg ccggggcggc ctgcgaagtg ctcggcgttg
     1801 tcggtgccgg agccggggtc ggcgaagtcg gaaccaccgg ggttgacgga tcgctcgtga
     1861 ccggggtcgt tggcgccgag cccggaacct ggccgactgt gcccgggtcg gtcgtggttt
     1921 cgctcggagc cgtggccggg ctctcgttca cgatctcgtc cggcgggtag gtggtgtggc
     1981 cctcgaggcc gtcgccgcct tccgaagtcg cgggcttggc cggcgtcaca ggttcggtcg
     2041 gcatggctgc ttccccggtt gcctgatcaa ctgcgggcgg tgccggagtg gtatcggttg
     2101 aagacggatc ggtggtcgcc gtatcgaccg gcgtggtgac aggggcctcg gccgaggcct
     2161 tctccagttc gggctcggcc ggcagggccg cgctccggtt ggtcggggtg gcgacgtgga
     2221 cgggcttggc cgggttggcg tcggcgatcg tcttggtcgt cgtggccgaa ctgctgtcga
     2281 ccgggctggt ggaaacctga tccttgacct cgaccgcgcc ggcgtcgtca ccgctgcg
//
LOCUS       DAOTIP010000319         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54274_length_2336_cov_3.259097, whole genome shotgun sequence.
ACCESSION   DAOTIP010000319 DAOTIP010000000
VERSION     DAOTIP010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54274_length_2336_cov_3.259097"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="P5138_06680"
     CDS             <1..234
                     /locus_tag="P5138_06680"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRV60298.1"
                     /translation="GPERPLTKPAHFGRFSGRRAKVTLAEPLDGRRNFTGTIAEAGSD
                     SVVLETDGQNFDIPYASIERSHLVPEIPQGAAK"
     gene            231..1925
                     /gene="nusA"
                     /locus_tag="P5138_06685"
     CDS             231..1925
                     /gene="nusA"
                     /locus_tag="P5138_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRV60299.1"
                     /translation="MTQEIVDAVRVLEQEKGIAADTLMDALADALLSAYRKSPGSAKY
                     ARVDLDHETGDFRVFELILPAELEEKLLEEAAAEQERTINPETGEYNEPEDPELDPEM
                     LEPYEDQIETRDVTPDDFGRIAAQTAKQVIYQRIREAERQMMYEEYRDRVGELITGLI
                     QQSDKRYTLVQLRERVEALLPKSEQVYSERYDHGMRVKAVITDVSESTKGPSIVVSRR
                     SPELIKGLFELEVPEIADKLVEIVGVAREPGYRSKIAVVSHADGVDPVGACVGPRGSR
                     VRMVVSELRGEKIDIIPYNEEPARFVAKALSPARVREVLVDDEDMQATVVVPDDQLSL
                     AIGRDGQNARLAARLTGWRVDIKSESEFAREESEHEYEGEEEFDGRCMAVLSSGRRCP
                     NAAVSGSTYCQLPQHQALSRFATSHVGVLASLSDEDISVLADPDADESAIGAIVSAAE
                     ADFVEPTEEEAPAEEAAVEEAEAETEAEELLVEEAEADIEADDEAAEEAVAEEEAGEE
                     LAAAEEAEAEAELEEAEAEVEAEEEVEAAPEPVDEAGEADQAVEAADEEAAEGEAE"
     gene            1922..>2336
                     /locus_tag="P5138_06690"
     CDS             1922..>2336
                     /locus_tag="P5138_06690"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRV60300.1"
                     /translation="MSKRRVHEIAKEAGVTSKELIAALKAAGVEVKAAASNVEEADAK
                     KALANLGGKTDEAKKPAAKPKAEAKPKAAAKPKAEAKPKAEAKPKTAAKPKAAPDGDT
                     KPSAGKVAAPKAPAKPAAAPKPAAKPAPASNKPVRT"
BASE COUNT          496 a          763 c          788 g          289 t
ORIGIN      
        1 ggccccgaga ggccgctgac caaaccggcc catttcgggc ggttttccgg acgtcgggca
       61 aaggtaaccc tggccgagcc cctggacggc cgcagaaact tcaccggaac cattgccgag
      121 gccggatcgg acagcgtcgt cctcgagacg gacggccaga acttcgatat tccttacgcg
      181 agcattgagc gctcgcacct cgtgccagaa atcccacaag gagcagccaa gtgacccaag
      241 agatcgtcga cgcagttcga gtcctcgaac aggaaaaggg aatcgccgcc gacaccctta
      301 tggatgcgct tgcggatgcg ctgctttccg cctaccgcaa gagccccggg tcggccaaat
      361 acgcccgtgt ggacctggac cacgagaccg gtgacttccg ggtcttcgaa ctgatcctgc
      421 ctgccgagct ggaagagaag ctgctcgagg aggctgccgc cgagcaggag cgcacgatca
      481 acccggagac cggcgagtac aacgaaccgg aggacccgga gctcgatccg gaaatgctcg
      541 agccctacga ggaccagatc gaaacccgtg acgtgacccc ggacgacttc ggccggatcg
      601 ccgcccagac cgccaagcag gtgatctatc agcggatccg cgaggccgag cgccagatga
      661 tgtacgagga gtaccgcgac cgggtcggcg agctgatcac cggcctgatc cagcagtcgg
      721 acaagcgcta caccctggtc cagctgcgcg agcgggtcga ggcgctgctc ccgaagtccg
      781 agcaggtcta cagcgagcgc tacgaccacg gcatgagggt caaggccgtg atcaccgatg
      841 tctccgagtc caccaaggga ccgagcatcg tcgtctcccg tcgcagcccg gaactgatca
      901 agggcctctt cgagcttgaa gtaccggaga tcgccgacaa gttggtcgag attgtcgggg
      961 tcgcccgcga gcccggctac cggtcgaaga tcgccgtggt ctcgcatgcc gatggcgtcg
     1021 atccggtcgg agcctgcgtc ggaccgcgag gttcgcgcgt ccgcatggtc gtttccgagc
     1081 ttcgcggcga gaagatcgac atcattccct acaacgagga gcccgcccgc ttcgtcgcca
     1141 aggcgctttc gcccgccagg gtccgtgagg ttctggttga cgacgaggac atgcaggcga
     1201 ccgtcgtggt tcccgacgac cagctttcgc tggcgatcgg ccgcgacggc cagaacgccc
     1261 gcctcgccgc ccgactcacc ggctggcggg tggacatcaa gtccgagagc gaattcgccc
     1321 gcgaggaaag cgagcacgag tacgagggcg aagaagaatt cgacggccgc tgcatggcag
     1381 tcctgtcgag cggacgccgc tgccccaacg ccgcggtatc cggctccacc tactgccagc
     1441 ttccccagca ccaggctctt tcccgcttcg cgaccagtca cgtcggagtg ctggccagcc
     1501 tcagcgacga ggacatctcg gtgctggccg accccgatgc cgacgagtcg gcgatcggtg
     1561 cgatcgtgag tgccgccgag gccgacttcg tcgagccgac cgaggaagag gctccggccg
     1621 aagaggccgc ggtcgaagaa gccgaagccg agacggaggc cgaagagctt ctggtcgagg
     1681 aagccgaggc cgacatcgaa gccgacgacg aggctgcgga ggaagccgtg gccgaagagg
     1741 aagccggcga ggaactcgcc gcagccgaag aagccgaggc cgaggccgag ctcgaggaag
     1801 ccgaagcgga agttgaagcc gaggaagaag tcgaagcggc ccccgagccg gtcgatgaag
     1861 ccggcgaggc cgaccaggcc gtcgaggcgg ctgacgaaga agctgcagaa ggcgaggccg
     1921 agtgagcaag aggcgcgttc acgaaatcgc caaggaagcc ggcgtaacca gcaaggagct
     1981 gatcgccgcg cttaaggctg ccggagtcga ggtcaaggcg gctgcctcca atgtggagga
     2041 agccgacgca aagaaggcgc ttgcgaacct cggcggcaag actgacgagg cgaagaagcc
     2101 tgccgccaag ccgaaggcag aggcgaagcc caaggccgcc gccaagccga aggccgaagc
     2161 caagccaaag gcagaggcga agccgaagac ggccgccaag ccgaaggctg cgcccgacgg
     2221 cgatacgaag ccgtcggccg ggaaggtcgc cgcgcccaag gcgcccgcca aacccgccgc
     2281 cgcgcccaag ccggccgcga aacctgctcc ggcctcgaac aagccggtcc gtaccg
//
LOCUS       DAOTIP010000320         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54343_length_2334_cov_2.371654, whole genome shotgun sequence.
ACCESSION   DAOTIP010000320 DAOTIP010000000
VERSION     DAOTIP010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54343_length_2334_cov_2.371654"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="P5138_06695"
     CDS             complement(<1..487)
                     /locus_tag="P5138_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60301.1"
                     /translation="MILSGENIPDYLDERPHLGDRVGTVTQVEMPDDGNINGVYIVQG
                     DRGSIVLKQGLPWVRIIETWELTAERTTREANLFEKWTPFAEGLLPEIFDFDADNHVL
                     AMEDLSGFAILADAFGADDQTANVERVGRLLARAFLNTSPFFLDPAEFSARLVEAENP
                     EL"
     gene            complement(505..1818)
                     /locus_tag="P5138_06700"
     CDS             complement(505..1818)
                     /locus_tag="P5138_06700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60302.1"
                     /translation="MIVGGGYVGLHTAIELARTAPGSRIIVLEREHCGFGASGRNGGQ
                     VHSWWNQIPTLSTICGREEAKLLADESVAAIERIGELSNRHPEVDFRQSGWLWTASTE
                     AQQGSWDACLAECDAQGVEPFAQLSGARLEEKTGTSVTRSGVFESTGGTVNPQGLVDA
                     LKQEATELGVEGFERTPVLEVSGHGPASVRTPNGDTAAGKVILTTNAWAARNRALQPY
                     LYVVGSDIVLTNPLHEISDEHLPEVGLAACDSQPRVLYWSLRGDRRLAFGRGGGRIAP
                     FNRIGRHSGGATRWRRDVITSMKRVYPKLGSAEPATAWSGAVDRTIDGFPIFGQTDRR
                     GPLYGVGWSGSGVVGSALGGRILASLACERDDFWSGNRLVTRRPIRLPREPLRTIGAH
                     IVRSAVRQSGKAEEQGTIPNPVTRKVAAMAPSLEADPESGPGAKQ"
     gene            complement(1904..>2334)
                     /locus_tag="P5138_06705"
     CDS             complement(1904..>2334)
                     /locus_tag="P5138_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV60303.1"
                     /translation="EADFPNVSQATPVYAIDDAAAVATKVSATLESIPDLAGIFASTE
                     AAAIGSVNALKSAGKDGDIPVVAYDGAPDEVQALKQGTISTLIVQKAYDMGKISVEQA
                     ADYVENGTKPDPEVTKTGIVVATKDNIDDPEVSKFLYPAK"
BASE COUNT          383 a          770 c          726 g          455 t
ORIGIN      
        1 ccagttcggg attttcggcc tcgacgagac gggcagagaa ctccgccggg tcgagaaaga
       61 agggcgaagt gttgagaaag gccctggcga gcaggcgacc cacccgttcg acgtttgcgg
      121 tctggtcgtc ggcaccgaag gcatcggcca ggatggcgaa ccccgaaagg tcctccatcg
      181 ccagcacgtg gttgtccgcg tcgaaatcga agatctccgg caggagtccc tcggcgaagg
      241 gagtccactt ttcgaacagg ttcgcttcgc gggtggtccg ttcggcggtc agctcccaag
      301 tctcgatgat tctgacccag ggcagtccct gcttcagaac gatcgaacca cggtcaccct
      361 gcacgatgta gacgccgttg atgttgccgt catcgggcat ctcgacctgg gtcaccgtgc
      421 cgacacggtc accgagatgc ggtcgctcat ccaggtaatc cggaatgttt tccccgctca
      481 gaatcatgaa gtcggccggt cgggtcactg tttcgctccc ggcccggact cgggatctgc
      541 ttccagcgac ggcgccatcg ctgccacctt cctggtgacc gggtttggaa tcgttccctg
      601 ctcttcggct ttgccgctct ggcgtacggc ggacctgacg atatgcgcgc cgatcgttcg
      661 cagcggctcg cgcgggagcc ggattggccg cctcgtgacg agccggttgc cgctccagaa
      721 gtcgtcacgc tcgcaggcga gcgaagcgag aatcctgccg ccgagggccg acccgaccac
      781 accgcttccg ctccagccga ccccgtagag gggaccccgc ctgtcggtct ggccaaagat
      841 cggaaacccg tcgatggtcc ggtccaccgc cccggaccag gctgtggcgg gctcggccga
      901 cccgagtttc ggatagacgc gtttcatcga agtgattacg tcccgacgcc atcttgtcgc
      961 tccgcccgag tgccggccga tgcgattgaa gggagcgatc ctgccgccgc cacggccgaa
     1021 tgcgagccgg cgatctccgc gaagcgacca gtagagcact ctcggctgtg aatcacacgc
     1081 cgccagtccg acctcgggaa ggtgttcgtc ggaaatctcg tgaagcggat tggtcagcac
     1141 gatgtccgag ccaaccacgt aaaggtacgg ctgaagtgct cggttccggg cggcccacgc
     1201 gttggtggtc aggatgacct tgcccgccgc ggtatccccg ttcggggtcc tgaccgacgc
     1261 cggtccgtgg ccggaaacct cgagtaccgg cgtcctttcg aagccctcga ccccgagttc
     1321 ggtcgcttcc tgcttcagcg cgtcaacaag tccctggggg tttaccgtcc caccggtaga
     1381 ttcgaatacc ccggaccggg tgacgctcgt tcccgtcttt tcttcaagtc ttgcgccgct
     1441 cagttgcgcg aagggttcga caccctgcgc gtcacactcg gcgagacatg cgtcccagct
     1501 tccctgctgt gcttcggttg aggccgtcca gagccagcct gactgcctga agtcgacctc
     1561 ggggtgccgg ttcgaaagct ccccgatccg ctcaatggcc gccaccgatt catcggcgag
     1621 gagctttgct tcctcgcgtc cgcagatggt cgacagggtc gggatctgat tccaccagct
     1681 gtgcacctgt cccccgttac ggccggaagc gccgaatccg cagtgttcgc gttcgagcac
     1741 gatgatccgc gatcccggtg cagtcctcgc cagctcgatc gccgtgtgga ggccgacgta
     1801 gccgccaccg acgatcacaa agtcggcctt tgaagggacc gggctccgcg ggcaactttc
     1861 gttgcccgcg gattcggtat ctggattgcc cggcctcact gacctacttg gccgggtaca
     1921 ggaacttcga gacttccgga tcgtcgatgt tgtccttggt ggcaacgacg atgccggtct
     1981 tggtgacttc cggatcgggc ttggtcccgt tctccacgta gtcggcagcc tgctcgaccg
     2041 agatcttgcc catgtcgtac gccttctgga cgatcagggt ggaaatcgtt ccctgcttca
     2101 acgcctggac ttcgtccggg gcgccgtcat aggcgaccac ggggatgtcg ccatccttgc
     2161 cggccgactt cagggcgttc acggaaccaa tcgccgccgc ctcggtcgag gcgaagatgc
     2221 cagccaggtc cgggatcgac tcgagcgtcg cggagacctt ggtggctacc gcagccgcat
     2281 catcaatcgc gtaaacgggc gtcgcctgac tgacgttcgg gaagtcggct tcca
//
LOCUS       DAOTIP010000321         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54374_length_2333_cov_2.841528, whole genome shotgun sequence.
ACCESSION   DAOTIP010000321 DAOTIP010000000
VERSION     DAOTIP010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54374_length_2333_cov_2.841528"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..458)
                     /locus_tag="P5138_06710"
     CDS             complement(105..458)
                     /locus_tag="P5138_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60304.1"
                     /translation="MWTAVKIGAIIGGVLLTLTFIAILIMVTRPAVLTGVAGPDLANS
                     VARIDQSSEGSCRKVSGGTWDCDVHGPNANGSFQVEVDWLGCWKAERTGPASGSGLPA
                     ERDGCIELGDIITFD"
     gene            complement(465..2045)
                     /locus_tag="P5138_06715"
     CDS             complement(465..2045)
                     /locus_tag="P5138_06715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRV60305.1"
                     /translation="MTSSKLLRSWGPPLAFIAVVTAVLLAIFPKGFPNYDTIYYLLWG
                     KEIAQGMSPDYGAPLAPTPHPLYDLLGAIFSPLGNGSITVAVVLAYVSLGLLAWLVYR
                     LGKEWFDRWIGYLAAFLVMTSAPVLSNGLRAYIDIPYMVLCLAALLIETRKPKAGWPV
                     LVLLTLAGLLRPEAWVFAGFYFLWLALDFHLAPANGPDDSAPERRPGVSVGRLRIQLR
                     ESGIDRNLILMACLTAAGPVIWILFDLITTSDPLYSLTGTQQTVETLERDTGPLDVII
                     YGPRRLGEVMQWPGMVGAFFGVILTLWTMPRRAAIGAISAAVVLLAFALMGASGLAII
                     PRYTMLAAAILFIFTAAAVLGWRLLEPVHRLRRVWQVAAAITLALWIIWLPNQYDLLS
                     TVDQDLSDQALVERDLEDLVDDGAFYANGTDDVRCLPISAPNHRAVPRLAFWLDIKPT
                     DVVSVAAEQQPRTGLFLAPAREFTIENFILDPGDETRTVTRPPSGFREVARNRSWILY
                     SNCPTGGSTGNAPLSTGP"
     gene            2130..>2333
                     /locus_tag="P5138_06720"
     CDS             2130..>2333
                     /locus_tag="P5138_06720"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV60306.1"
                     /translation="MSESTSLYRRHRPQSFDQVVGQQHVVRTLSNAITQDKVHHAYLF
                     VGSRGTGKTSMAKILAASLNCENG"
BASE COUNT          438 a          717 c          814 g          364 t
ORIGIN      
        1 gagcagcacg cccgggtctg aacgcccgaa gcaaccgctg ggctgaaggt ccgaagcggc
       61 tcggtgcaga tcgcccccta tatagagggg taattcgcac cgagtcagtc gaaagtgatg
      121 atgtcgccga gctcgatgca gccgtcgcgt tcggcaggga gacctgaccc ggaggcggga
      181 ccggttcgct cggccttcca gcatcccagc cagtcgacct cgacttggaa ggatccgttg
      241 gcgttcggtc cgtggacatc gcagtcccag gttccgccgg acaccttgcg gcaggagccc
      301 tcgctcgatt ggtcgattcg cgccacggaa ttggccaggt cggggcccgc gacaccggtc
      361 aggacggcgg gccgggtgac catgatcagg atcgcgatga aggtgagggt gagcaggacc
      421 ccgccgatga tcgccccgat cttgaccgcg gtccacatcg cgggtcaggg gccggtcgaa
      481 agcggggcgt tgccggtcga gccaccggtc gggcagttcg agtagagaat ccaggaacgg
      541 ttgcgggcca cttccctgaa cccggacggc gggcgggtca cggttctcgt ttcatcgccc
      601 gggtcgagga tgaagttctc gatggtgaac tcgcgggcgg gtgcgaggaa gagtccggtc
      661 cggggctgct gttcggcggc caccgagacc acgtcggttg gcttgatgtc gagccagaaa
      721 gcgagtcgtg gtaccgcccg gtgattaggc gccgagattg gcaggcagcg cacatcgtcg
      781 gtgccgttgg cgtagaaggc gccgtcgtcg accagatcct cgaggtccct ctcgaccagg
      841 gcctgatcgc tcaggtcctg gtccaccgtg ctgagcaggt cgtactggtt gggcagccag
      901 atgatccaga gggctagcgt gatcgcggcg gcgacctgcc atacgcggcg cagccggtgg
      961 accggttcga gcagtcgcca gccgagcacc gcggcggccg tgaagatgaa gaggatcgcg
     1021 gcggcgagca tcgtgtagcg ggggatgatc gccagcccgg aagcacccat caacgcgaac
     1081 gcgagcagga ctacggcagc ggaaatggcg ccgatcgccg cccttcttgg catcgtccag
     1141 agcgtcagga tcaccccgaa gaaggcaccg accatgcccg gccactgcat tacttccccg
     1201 aggcggcggg gcccgtagat gatcacgtcg agcgggccgg tgtcgcgctc gagcgtctcg
     1261 accgtttgct gggttccggt cagcgagtag agcgggtcag aggtcgtgat caggtcgaag
     1321 aggatccaga tcaccggccc ggccgcggtg agacaggcca tcaggatcag gttccggtcg
     1381 atgccggact cgcgaagctg aatccggagc cggccgacgc tgacaccggg ccttcgttcc
     1441 ggggccgaat cgtcaggacc gttggccggg gcgaggtgga agtcaagggc cagccagagg
     1501 aaatagaagc cggcgaagac ccaggcctcg ggtctgagca gaccggccag ggtgagcagg
     1561 acaagcactg gccagcccgc cttcggcttc ctggtctcga tcagcagcgc ggcgaggcag
     1621 aggaccatgt acgggatgtc gatatacgcc ctgagtccgt tactgaggac cggggcactg
     1681 gtcatgacca ggaatgcggc cagatagccg atccagcggt cgaaccactc cttgcccagc
     1741 cggtagacca gccaggccag caaaccgagg ctgacgtagg ccagcaccac tgcgaccgtg
     1801 atcgaaccgt tgccgagcgg cgagaagatc gcgccgagca ggtcgtagag gggatgcggg
     1861 gtgggagcca gcggggcgcc gtagtccggg ctcatgccct gggctatctc cttgccccag
     1921 agcaggtaat agatcgtgtc gtagttgggg aaacccttcg ggaagatcgc cagaaggacc
     1981 gccgtgacga cggcaatgaa ggcgagcggc ggtccccagg accggagcag tttcgacgac
     2041 gtcatccggg ccattctgac ccatgaacca gactcacggt tcgcccgttg ggctgtcgcg
     2101 cgggacggct acactggctt tcttccccga tgagtgaatc gacctccctt taccggcgcc
     2161 atcgacccca gtccttcgac caggtggtcg gccagcagca tgtggtccgg accctgtcca
     2221 acgcgatcac ccaggacaag gtccaccacg cctacctgtt cgtcggatcg aggggaaccg
     2281 gcaagacctc gatggcgaag atcctggccg cttcgctcaa ctgcgaaaac ggc
//
LOCUS       DAOTIP010000322         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54379_length_2333_cov_2.607112, whole genome shotgun sequence.
ACCESSION   DAOTIP010000322 DAOTIP010000000
VERSION     DAOTIP010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54379_length_2333_cov_2.607112"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="P5138_06725"
     CDS             complement(<1..1128)
                     /locus_tag="P5138_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60307.1"
                     /translation="MPGSKSLHSPRFVLAGCCLALVLVMASVTSVNLALEGIAVDLGA
                     AGSDLTWIADAYTVALAAFVLPFGALGDDKGRKNTLVVGTIVFGIAAGVAATSSSVGF
                     LIACRAVMGIGAALIMPSTLSTVTSVYSEEGKARAVAIWAGFASAGAVLGLLMSGLLL
                     EYFSWESTFLAVAILAAISLVATLVFIPHTSDEEESHPDPVGAGLTAIGIGALVYGVI
                     EGADAGWGATAPVVAFIVAAVAILGWAIHDSRVTWPMLDPRLFRLRGLSSGSLTLVLI
                     FLGAFGFFYVGLQYVQLVLGFGPLLAAVSFLPIAVVVMPVSALTPRLVERWGDKPLMA
                     GGLILMGVAFYLITGLTAESEYPSFLVPMCVFAFGMALASTP"
     gene            complement(1219..1980)
                     /locus_tag="P5138_06730"
     CDS             complement(1219..1980)
                     /locus_tag="P5138_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV60308.1"
                     /translation="MANRLEGAVALVTGASSGIGEATAKSLAAQGAKVALVARRADRI
                     ERLAQEIAANGHTALAIEADVTDQEQAIAAVERTAGDFGRLDIVVNNAGVMLLGPIHG
                     APTEEWDRMIDVNLKGLLYVTHAALPHLFAATADSPRKVADVVNISSVAGRVVNAGAG
                     VYNLTKHGVVAVSEALRQEASRGGVRVTIIEPGFVETELQGHNRPEVQEALKERVSKI
                     EPLQSHDIADAIEYAVTRPAHVSMNELLIRPTTQP"
     gene            complement(2057..>2333)
                     /locus_tag="P5138_06735"
     CDS             complement(2057..>2333)
                     /locus_tag="P5138_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60309.1"
                     /translation="RRQLDGLLAGSDSARDSIANDPASVQQQIESIAREAFTFALSNS
                     IWVLVGIAVVCTGLTAWLVEPKEPTSELPEAAQQLEHKHHRFGGFHL"
BASE COUNT          431 a          750 c          779 g          373 t
ORIGIN      
        1 gggggtgctg gccagggcca tgccgaatgc gaacacgcac atcggaacca ggaaggacgg
       61 gtactcggac tcggcggtca ggccggtgat caggtagaag gcgacgccca tcaggatcag
      121 cccacctgcc atcagcggct tgtcacccca tctttcgacc agcctcgggg tcagggccga
      181 gaccggcatg accacgaccg cgatcggcaa gaacgagacg gcggcgagga gcgggccgaa
      241 gccgagcacg agctggacgt actggaggcc gacatagaag aagccgaagg ctccaaggaa
      301 tatcaggacc agggtcagcg aaccactgga aagtcccctg agcctgaaaa ggcgcggatc
      361 gagcatcggc caggtgaccc ggctgtcgtg gatcgcccag ccgagaatcg ccacggccgc
      421 gacgatgaag gcgaccaccg gcgctgtggc gccccagccg gcatcggcgc cttcaatcac
      481 cccgtagacg agggcgccga tcccgatcgc ggtcagcccg gccccgaccg ggtcgggatg
      541 ggattcctct tcgtcgctgg tgtgcggaat gaagaccagc gtcgcgacca gggagatcgc
      601 cgcgaggatg gccaccgcaa ggaaggttga ctcccaggag aagtattcga gcagcaggcc
      661 gctcatgagc agcccgagga cagcgccggc gctggcgaat ccggcccaga tcgcaacggc
      721 gcgggccttg ccctcctcgg agtagacgga ggtcaccgtg gaaagggtgc tcggcatgat
      781 cagggcggca ccgatgccca tcacggcacg gcaggcgatc aggaagccga ccgaactcga
      841 ggtggcggcg acgccggccg cgatcccgaa gacgatggtg ccgacgacca gggtgttctt
      901 gcggcccttg tcgtcaccga gagcgccaaa gggaagcacg aaggcggcca gggcaacggt
      961 gtaggcgtcg gcgatccagg tgagatcgga tccggcggca ccgagatcaa ctgcgattcc
     1021 ctcgagggcg aggttgacgc tggtgaccga tgccatgacg agcacgaggg ccagacagca
     1081 tccggcgagg acgaaacggg gactgtggag actcttcgaa ccgggcatgt tcgggttata
     1141 ctacaaatgt agtaactggg caagaaagag gcccgggaac tttgcccggg cctctttcga
     1201 actcctgtct gcagtcgttc agggttgtgt cgtcgggcgg atcagcagtt cgttcatcga
     1261 cacatgggcc gggcgggtca ccgcgtactc gatcgcgtcg gcgatgtcat gggactggag
     1321 cggttcgatc ttggagaccc gctccttcag cgcctcctgg acctcgggcc ggttgtgccc
     1381 ctgcagctcg gtctcgacga agcccggctc gatgatcgtc acccgcaccc cgccccggct
     1441 ggcttcctgg cgaagtgcct cgctgaccgc cacgaccccg tgcttggtca ggttgtagac
     1501 cccggctccg gcgttgacca cccgccccgc gaccgaggag atgttcacga catcggccac
     1561 cttccgcggg gaatcggccg tggccgcgaa gagatgcgga agggcggcgt gggtcacata
     1621 gagcagtccc ttgagattga cgtcgatcat ccggtcccac tcttcggtcg gggcgccgtg
     1681 gatcggcccg agcagcatca ccccggcgtt gttcacaacg atgtcgagcc gtccgaagtc
     1741 accggccgtg cgctcgaccg ccgcgatcgc ctgctcctgg tcggtcacgt cggcctcgat
     1801 cgcgagcgcg gtgtgaccat tggctgcgat ctcctgggcg agtctctcga tccggtccgc
     1861 ccggcgcgcg accagcgcga ccttcgcacc ctgtgccgcc agcgacttgg ccgtcgcctc
     1921 tccgatgccg ctgctcgccc cggtcaccag ggcaactgcg ccttcaagtc tgttcgccac
     1981 ttttttcctc cgttagctag ccgttcaccc cgaggatatc cccgtcaaat ctccggtcgg
     2041 ccacgggcgc ggatgactag aggtgaaaac cgccgaaacg gtggtgcttg tgttcgagct
     2101 gttgggctgc ctcgggaagc tcactggtcg gctccttcgg ttcaacgagc catgccgtca
     2161 agccggtaca tacgaccgct atcccgacca ggacccagat cgaattgctc agagcgaaag
     2221 tgaacgcttc ccgggctatc gactcgatct gctgctggac gctcgcggga tcgttggcaa
     2281 tcgaatcgcg agctgaatcg gaaccggcga ggaggccgtc cagctggcgg cgt
//
LOCUS       DAOTIP010000323         2331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54431_length_2331_cov_4.252636, whole genome shotgun sequence.
ACCESSION   DAOTIP010000323 DAOTIP010000000
VERSION     DAOTIP010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54431_length_2331_cov_4.252636"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="P5138_06740"
     CDS             <1..688
                     /locus_tag="P5138_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRV60310.1"
                     /translation="LHGGAALALLITRRGEIRTELAEFDLRRASVLLFSFLPAAFVGY
                     RFERVIESKFGGPRGTAAGLILGGLAMAIADRRPQDRGKGEADWKDGLALGVAQAAAL
                     IPGVSRNGATLTAARLRRFSRDQANLLSRTVALPVILGAVGLKGVRLARRGISGRQAR
                     WLAIGTATSFASTMAATDLIRMVERDRALAPYGAYRIAFALLILSRLAAERRNRSSSL
                     PVAPDTRTAE"
     gene            716..1732
                     /gene="purM"
                     /locus_tag="P5138_06745"
     CDS             716..1732
                     /gene="purM"
                     /locus_tag="P5138_06745"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRV60311.1"
                     /translation="MSGEDAYAKAGVSQSDADLAVSRLVAALATADIGRPTRQVDLKG
                     HYAAVMRLDNHTGIALSTDGVGTKLLVAEELGRFDTVGIDCIAMNVNDIICVGAEPFA
                     MLDYLAVEQADPAMCEQIGVGLARGAELAGIEIPGGELAQIGDLVKGFDIAGACFGTV
                     ALDSIIDGSAVAPSDVVIGLPSSGIHSNGYTLARSALSGIAMADERLGRPLGEVLLEP
                     TEIYVRAVLDLLASPARVHGLAHITSGGLDNLLRLKADVTYEITDPLAPQPIFELIAE
                     RASVPEDEMYEVFNMGCGFCVVVPAEDEATALDVLKAHYPEARRIGDVADGDRKVIRR
                     SSDS"
     gene            1908..>2331
                     /locus_tag="P5138_06750"
     CDS             1908..>2331
                     /locus_tag="P5138_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRV60312.1"
                     /translation="MNHPPASSQMIGTVLSDRYRLEAKLGSGGMSTVYLARDEVLDRP
                     VAVKLMHREMTEEPEQLERFNQEARAVAKLSNPNVVAVIDAGEDQGRPYIVLEYVQGE
                     TLKQRISRVGALDATEALAYGLEVAQGLGVAHERNMVHR"
BASE COUNT          376 a          804 c          756 g          395 t
ORIGIN      
        1 cctccacggc ggcgccgcgc tggccctgct gatcacccgc cggggggaga tccggacaga
       61 actggctgaa ttcgaccttc gacgcgcctc ggtcctgctc ttctcctttc tcccggctgc
      121 cttcgtcggc taccgattcg aacgggtgat cgagtcgaag ttcggcggac cgcggggcac
      181 cgctgccggc ctgatcctcg gcggcctggc gatggcgatt gccgaccgcc ggccgcagga
      241 ccgtggcaag ggcgaagcgg actggaagga cggcctggct ctcggagttg cacaggctgc
      301 cgccctgatc cccggggtgt cccgcaacgg tgccaccctg accgccgccc ggctgcggcg
      361 cttctcgcgg gaccaggcca acctgctgtc ccggacggtc gcactgccgg tgatcctcgg
      421 cgcggtggga ttgaagggag tccggctcgc ccggcgggga atctccgggc gtcaggcccg
      481 ctggctggcg atcggcaccg ccacctcttt cgcttcgacc atggccgcca ccgatctgat
      541 caggatggtc gaaagggacc gcgcgctcgc cccctacggc gcctaccgga tcgccttcgc
      601 actgctgatc ctgtcccggc tggcggccga acggaggaac cggagttctt cacttccggt
      661 ggctccggac acccggaccg ccgaatgacc gcgctctcat gttgaatcac tcccgatgag
      721 cggcgaagac gcatacgcca aggcgggagt cagccagtcc gacgccgacc tcgcggtaag
      781 ccgcctggtc gcggccctgg ccacggccga catcggccgc cccacccggc aggtcgacct
      841 caagggccac tacgccgcgg tgatgcggct ggacaaccac acgggcatcg cgctctccac
      901 cgacggagtc ggcacgaagc tgctcgtcgc cgaggaactc ggccgcttcg acacggtcgg
      961 gatcgactgc atcgcgatga acgtcaacga catcatctgc gtcggcgcgg aaccgttcgc
     1021 catgctcgac tacctcgcgg tcgagcaggc cgacccggcg atgtgcgagc agatcggagt
     1081 cgggctggct cgcggggccg agctggccgg gatcgagatc cccggcgggg agctcgccca
     1141 gatcggtgac ctggtcaagg gctttgacat cgccggcgcc tgtttcggaa cggtcgccct
     1201 ggactcgatc attgatggct cggcggtcgc cccgagcgac gtcgtgatcg gacttccctc
     1261 ctccggcatc cattcgaacg gctacacgct ggcccgttcg gctctttccg gcatcgccat
     1321 ggccgacgaa cggctgggcc gtccgctggg ggaagtcctg ctcgagccga ccgagatcta
     1381 tgtaagggcg gtgctcgacc tgctcgcttc gcccgcccgg gtgcacggac tcgcgcacat
     1441 cacctccggc ggactcgaca atctcctgcg cctcaaggcc gatgtgacct acgagatcac
     1501 cgatccgctc gcgccccagc ccatcttcga gctgatcgcc gagcgggcga gcgtgccgga
     1561 agacgagatg tacgaggtct tcaacatggg ttgcggcttc tgcgtggtgg tcccggccga
     1621 agacgaggcg accgccctcg atgtgctgaa ggcccattat ccggaggccc gccgaatcgg
     1681 cgacgtcgca gacggtgacc gcaaggtcat ccgccgatcc tcagattcct agtcctttgc
     1741 tgcatttttg ttggcatcag ccaacacttc agacctcctt caggcggttc atactggact
     1801 tgaagcaact tgacgaattt ttcccgagac tgccctttct gctgcggatt ctccgcgctc
     1861 tggatagcct gcggcgccta tgagcggtcg caggcaaaat cagtgaagtg aaccacccgc
     1921 ctgccagctc acagatgatc ggaacggtcc tttcggaccg ttaccggctt gaggccaagc
     1981 tgggcagcgg cggcatgtcc actgtctatc tcgcccgtga cgaggtgctc gaccgtcccg
     2041 tcgcggtgaa gctgatgcac cgggagatga ccgaggagcc cgaacagctc gagcgcttca
     2101 accaggaagc gcgggcggtg gcgaaactct cgaacccgaa cgtggtcgcc gtgatcgacg
     2161 ccggcgagga ccagggccgg ccctacatcg tgctcgaata cgtccagggc gaaacgctca
     2221 agcagcggat ctcgcgggtc ggtgccctcg atgcgaccga ggcgctcgcc tacggactcg
     2281 aggttgccca ggggctcggt gtcgcccatg agcgcaacat ggtccaccgg g
//
LOCUS       DAOTIP010000324         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54527_length_2328_cov_3.986802, whole genome shotgun sequence.
ACCESSION   DAOTIP010000324 DAOTIP010000000
VERSION     DAOTIP010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54527_length_2328_cov_3.986802"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5138_06755"
     CDS             complement(<1..255)
                     /locus_tag="P5138_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HRV60313.1"
                     /translation="MSEKCEHLDEVRVTRLPESVEGCEDCLREGTGWVHLRICLVCGH
                     VGCCDNSPMRHATGHFEETGHPLIRSIEPGEEWSWCYEDEI"
     gene            complement(252..2153)
                     /locus_tag="P5138_06760"
     CDS             complement(252..2153)
                     /locus_tag="P5138_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRV60314.1"
                     /translation="MSIDRKGATDYTRDAHVHSVRCLDFEDKASFEEAKRGFIAPVPE
                     GQVLKEDGDFVFDPHKLAFASGEPEEPETVNPSLWRQARLYADGGLFEVCDRIYQVRN
                     LDISNITFIEGDTGLIVVDPLVSAEVAKAALELYFEHRGEKEVVAVIHSHSHVDHFGG
                     VRGVVDQADVDSGKIPIIAPEGFLEAAVSENVAAGNVMTRRAMYQFGILLPPDVQGTV
                     GSGLGIGTSLGTITLIPPTDLITYTGETREIDGLTFEFMLTPDTEAPAEMHWYIPELR
                     ALTAAENCTHTLHNTYPIRGAIVRDPQAWSMYLNDTIDRWADKSDVMYAMHHWPVWGK
                     DRVREMLEKGRDAYRFIHDETLRLANHGLTPREIAEQVEFPDSLAKHWSVRSYYGTVS
                     HNVKSTYTRYLGWYDGNPANLEPMVPVESGKRYVEFMGGAENVVKMAGESFEKGEYRW
                     VVEVLGHVVFADPSNSEARELQADAFEQLGYQTESSTWRNAYLMGAQELRHGTPSFPI
                     GSTASEDSIRAMTLPMIFNFFAIKIHGVNASDLELDLNLDITDTGESAVLRLSNGVLS
                     HSLVDRDEDAPVLAMTRDLLNRLVGNEITLDEAVEAGEVEAGAGLEVFRKFGEHLDEF
                     DLWFPIIEP"
BASE COUNT          395 a          757 c          710 g          466 t
ORIGIN      
        1 gatctcgtcc tcgtagcacc aggaccattc ctcgccgggc tcgatcgagc gaatcagcgg
       61 atgtccggtc tcttcgaaat gaccggtggc gtgccgcatc ggcgagttgt cacagcagcc
      121 gacatgaccg cagaccagac agatccgcag gtggacccaa cctgtcccct cccgcaggca
      181 gtcttcgcag ccttcgaccg attcaggcag ccgggtgacc ctgacctcgt ccaggtgttc
      241 gcacttttcg ctcatggctc gattatcggg aaccagaggt cgaactcgtc gaggtgctca
      301 ccgaacttgc ggaatacctc gagtccggcg cccgcttcca cttcccccgc ctcgaccgcc
      361 tcgtcgaggg tgatctcgtt tccgaccaga cggttgagca ggtcccgggt catcgccagc
      421 accggcgcat cctcatcccg gtcaacaagc gaatggctca gcaccccgtt ggaaaggcgc
      481 aacactgccg actcgccggt gtcagttata tccaggttca ggtcgagttc caggtcggat
      541 gcgttgaccc cgtggatctt gatcgcgaag aaattgaaga tcatcggcag ggtcatcgcc
      601 cggatcgaat cctcgctcgc cgtgcttccg atcggaaaac tgggcgtccc gtgccggagc
      661 tcctgggcac ccatcaggta ggcgttccgc caggtcgacg attccgtctg gtaccccagc
      721 tgctcgaagg catcggcctg cagctccctg gcctcgctgt tcgaggggtc ggcgaagacc
      781 acgtggccga gtacctcgac cacccagcgg tactcgccct tctcgaacga ctccccggcc
      841 attttcacca cgttctcggc gccacccatg aactcgacgt agcgcttgcc ggattcgacc
      901 gggaccatcg gctcgaggtt ggccggattg ccgtcgtacc agcccagata gcgggtgtaa
      961 gtcgacttga cgttgtggct gaccgtgccg taataacttc gaaccgacca gtgcttcgct
     1021 agcgaatccg ggaattcgac ctgttcggca atctcgcgcg gggtcaggcc gtggtttgcg
     1081 agtcgcaggg tctcgtcgtg gatgaagcgg taggcatcgc ggcccttctc gagcatctcg
     1141 cgaacccgat ccttccccca gaccggccag tgatgcatcg cgtacatgac atccgacttg
     1201 tcggcccagc ggtcgatcgt gtcgttcagg tacatcgacc atgcctgcgg atcacggacg
     1261 atcgcgccgc ggatcgggta ggtgttgtga agggtgtggg tgcagttctc ggccgcggtc
     1321 aaggcccgaa gctccgggat gtaccaatgc atctcggccg gcgcctcggt atccggggtg
     1381 agcatgaact cgaaggtaag accgtcgatc tcgcgggtct cgccggtgta ggtgatcagg
     1441 tcggtcggcg ggatcagggt gatcgtgccc agcgaagtgc cgatgccgag cccgctgccc
     1501 accgtgccct ggacgtcagg cggcagcagg atcccgaact ggtacatggc ccgccgggtc
     1561 atcacgttgc cagccgccac attctcggaa actgccgctt cgaggaagcc ctccggagcg
     1621 atgatcggga tcttccccga gtcgacgtcc gcctggtcga ccaccccgcg gactccgccg
     1681 aaatggtcga cgtggctgtg cgaatggatc accgcgacca cttccttttc gccgcgatgc
     1741 tcgaagtaga gctcgagggc cgccttggcc acctcggccg agaccagcgg gtcgaccacg
     1801 atcaggccgg tgtcaccctc gatgaaggtg atgttcgaaa tgtccaggtt gcggacctgg
     1861 tagatccggt cgcagacttc gaagagcccg ccgtcggcgt aaagccgggc ctggcgccag
     1921 aggctggggt tgaccgtttc cggctcttcc ggctcgccgg aggcaaaggc gagcttgtgg
     1981 ggatcgaaga cgaaatcgcc atcctccttg aggacctgcc cctcggggac cggcgcgatg
     2041 aacccgcgct tcgcttcttc gaaagaggcc ttgtcctcga agtcgagaca acggacgctg
     2101 tggacgtgag cgtccctggt gtagtcggtt gcgcccttgc ggtcgatgct cattgcctct
     2161 cctctttggt tgtcgtggtg acagttaccg cggcggtgac cacggtctct tcctcgagtt
     2221 cctgcagcgc cgtttccatt tcggccgctt ccattcttcc agcactgggt cccatttcaa
     2281 cgagatcccg aacctcgctc cggctgaggc tcatgctcca ctcaagtt
//
LOCUS       DAOTIP010000325         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54548_length_2328_cov_2.375715, whole genome shotgun sequence.
ACCESSION   DAOTIP010000325 DAOTIP010000000
VERSION     DAOTIP010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54548_length_2328_cov_2.375715"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5138_06765"
     CDS             complement(<1..250)
                     /locus_tag="P5138_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HRV60315.1"
                     /translation="MSDGWSSTTYTEWRETCDTLHAHTQFLGKLCVALAPPEPQLQHA
                     ALRLTARGWETLPLPAPDGSGVFVVALDLRTHHLLVEHS"
     gene            complement(282..794)
                     /locus_tag="P5138_06770"
     CDS             complement(282..794)
                     /locus_tag="P5138_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60316.1"
                     /translation="MPFAELLRATVFLTAGGATALGVVAVIGVQDAENTTVLLIGAAW
                     WLVAAWIGLSLGGAERSADSMRETLAGARTATSSSPGVSMPSPGRVAWGRLSPILAAV
                     GLAGALGVIFPEVAMVGSGYALLVALAWRNRESAVLAIEGRDGVRFLVESASPLKPVK
                     LVRSPGFRAF"
     gene            complement(798..1577)
                     /gene="otsB"
                     /locus_tag="P5138_06775"
     CDS             complement(798..1577)
                     /gene="otsB"
                     /locus_tag="P5138_06775"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRV60317.1"
                     /translation="MSDVDGTLCRIVDRPDQARVPVRARESLETLADHLALVACITGR
                     PSPVARKMVGAEKITYFGNHGLSFIKPGQEKPTVVIEGEEAERARKFVRDNDNPFWVT
                     SKIRIEDKGPIQALHWRGAGHRTEEMVRIIADHAQSAGLATHWGRKVLELRPPGMEGK
                     AGAVDELLDGTDIETVVFAGDDRTDFDAAARLKKLADEGKLKNLLLVAIDSDEGPPEL
                     IEMADLVVAEPEEWIDLIEDFAAGFSEAPVEAPQPGAPQPE"
     gene            1655..2020
                     /locus_tag="P5138_06780"
     CDS             1655..2020
                     /locus_tag="P5138_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRV60318.1"
                     /translation="MESRRIIGLAAVGTLAGAFSGLFGVGGGTVIVPMLIALYHYGDR
                     LATGTSLAAIIVIALLAAAMQGGIYGNVDVGTGLILAGPAIVGVIGGTAIQQRIPEKS
                     VSLIFAVLLIGVAIEMVVG"
     gene            2020..>2328
                     /locus_tag="P5138_06785"
     CDS             2020..>2328
                     /locus_tag="P5138_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRV60319.1"
                     /translation="MDIAIACLICFIGGLVAGLIGVGGGIVFVPAMTAVLSKGQVEAE
                     STSLLMIALVSVVGTWRQIGYGNVNLKDAAVIGLLSPVGVLIGVVAANALPERALRIG
                     F"
BASE COUNT          374 a          760 c          801 g          393 t
ORIGIN      
        1 ccgaatgctc gaccagcaga tggtgggtgc gcaagtcgag cgccaccacg aagacaccgg
       61 agccgtccgg ggcgggtaac ggaagggttt cccagccgcg ggcggtgagc cggagcgccg
      121 catgctgaag ctgcggctcc ggcggggcaa gggcaacaca gagcttgccg aggaactggg
      181 tgtgagcgtg aagcgtgtcg caggtctcac gccactcggt gtaggtggtg gaactccaac
      241 cgtcagacat gtcccgaccc tactcccccg gaaagccctg ctcagaaggc tcggaagccg
      301 gggcttcgga ccagcttgac gggcttcagc ggcgaagccg actcgaccag aaaccgcacg
      361 ccgtcccttc cctcgatcgc gagcaccgcc gactcgcggt ttcgccaggc cagggcgacc
      421 agcagggcgt agccggagcc gaccatggcg acctcgggaa agatgacccc gagagcgccg
      481 gcgaggccga ccgcggcgag gatcggcgag agccggcccc aggcaacccg gcccggggag
      541 ggcatggaaa caccgggcga agacgaggtg gcggtgcgag ccccggcaag ggtctcgcgc
      601 atcgagtcgg cggacctttc ggcgccgccg aggctgaggc cgatccatgc ggcgaccagc
      661 caccaagcgg ccccaatcag gagcaccgtt gtgttctcgg cgtcctgaac gccgatcacc
      721 gcgaccacac cgagcgcggt cgcaccgccc gcggtgagaa agacggtggc cctgagcagc
      781 tcggcgaagg gcaagcccta ctccggctgt ggggcacccg gctggggggc ttcgaccggc
      841 gcttcgctga acccggccgc gaagtcctcg atcaggtcga tccactcctc cggttccgcc
      901 accaccaggt cggccatctc gatcagttcg ggcgggcctt cgtcggaatc gatcgcgacc
      961 agcagcaggt tcttcagttt tccttcgtcg gcgagcttct tgagccgcgc tgccgcatcg
     1021 aaatccgtgc ggtcatcccc ggcgaacacg acggtttcga tgtcggttcc gtcgagcagc
     1081 tcgtccacgg cgccggcctt gccttccatg cccgggggcc ggagctcgag caccttgcgg
     1141 ccccagtgag tggccaagcc ggccgactgg gcatggtcgg cgatgatccg gaccatctct
     1201 tcggtgcgat gcccggcgcc ccgccagtga agggcctgga tcggtccctt gtcctcgatc
     1261 cggatcttcg aggtgaccca gaacgggttg tcgttgtcgc gcacgaactt cctcgcccgc
     1321 tccgcctcct ccccttcgat caccacggtg ggtttttcct gccccggctt gatgaagctg
     1381 aggccgtggt tgccgaagta ggtgatcttc tcggcgccga ccatcttccg ggcaaccggc
     1441 gagggacgac cggtgataca ggcgaccagg gccaggtgat cggccaaggt ttcgaggctt
     1501 tcccttgccc tgaccggaac gcgggcctgg tccggtctat cgacgatccg gcagagcgtg
     1561 ccatcgacat cgctcatcag agccgacttt gaggggtctt ccaagagtac ggagagaggg
     1621 gagtctgagg cggccatggt cgtagtatcg cgggatggag tcacggcgca taatcggact
     1681 tgccgcggtc ggcactttgg ccggcgcatt cagcggactt ttcggggtcg gcggcggcac
     1741 cgtgatcgtg ccgatgctga tcgctctcta ccactacgga gaccgactgg cgaccggaac
     1801 cagcctcgcc gcgatcatcg tgatcgccct gctggcggcg gcgatgcagg gcgggatcta
     1861 cggcaacgtc gatgtgggaa cgggactgat cctggccggt ccggcgatcg tcggtgtgat
     1921 cggcgggacc gcgatccagc agcggattcc ggagaagtcg gtctccctga tcttcgccgt
     1981 cctcctgatc ggcgtcgcga tcgaaatggt ggtcggctga tggacatcgc tatcgcctgc
     2041 ctgatctgtt tcatcggcgg cctggtcgcc ggcctgatcg gggtcggcgg agggatcgtc
     2101 ttcgtgcccg cgatgaccgc ggtcctttcg aagggtcagg tcgaggccga gtcgacctcg
     2161 ctgctgatga tcgccctggt ttcggtggtc ggaacctggc ggcagatcgg ctacggaaac
     2221 gtgaacctca aggacgccgc ggtgatcggc ctgctctcgc cggtcggagt cctgatcgga
     2281 gtggttgcgg ccaacgcgct acccgagcgc gccctgcgca tcggcttc
//
LOCUS       DAOTIP010000326         2326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54593_length_2326_cov_3.537649, whole genome shotgun sequence.
ACCESSION   DAOTIP010000326 DAOTIP010000000
VERSION     DAOTIP010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54593_length_2326_cov_3.537649"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1314
                     /locus_tag="P5138_06790"
     CDS             100..1314
                     /locus_tag="P5138_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HRV60320.1"
                     /translation="MSLGRRLRRRYQVKILTVLRFLPDRSGAPPVPPPWDPFEREIPE
                     DLLTSPGELRDPVEEERIAREEPLHEFFSVHPEASEFIVAHGRGFLQSTFTRLARGVH
                     RIRRATRFRPSPNRSPASAAEMTSILRLEAERIGLSRIGIAPNDPKYTFPESDAKQLP
                     NVVVCLVEQDWELTQMIPSDEAEKSAMYGYSEGMTRAAQLAEFLKRMGFDAAPQGFPG
                     DAITIHYGVASGLGQLGLNGQLLTPEAGSRCRILLLTTDAPLTHDSPVDYGLHKVCDE
                     CKACVRNCPVGAIPQTRRPYRGVVKSKLNTVRCLPVVAQASGCAVCMKVCPVQRYGLE
                     AILDHREQTGEILGIGSDELEGYDWPLDGRHYGPGETPKVGDEVLNNPDLVFDFSRKE
                     PPPGIGPIRFVG"
     gene            complement(1357..>2326)
                     /locus_tag="P5138_06795"
     CDS             complement(1357..>2326)
                     /locus_tag="P5138_06795"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HRV60321.1"
                     /translation="SPNGSCNGATPTDAGYNIDDDGTCISPTMPGTGSHAGTTAYGSS
                     TYGEVLDSYLADVAAFNGGQSRTFALLNSPDPATDLANPALAVVPTDYDFPTPVSGES
                     NACSLADQRGVLPAAGIACDMGAYLLQQTEIELTASASSVEQNQPVTYTATITPAPDG
                     GTVSFDDGAGNPATVNCPAEYLANGAATCTVSYPDPGTYPVTASYTGDGDRNNFTASQ
                     SVVPTNVTTEVTPTPPPVVDRPLRLLGAIKFPKNGYNKIRVKIPAAGQVELIGYKRVK
                     GSSAHFDQAGTYLIKAVPKGPLLRKLNRRGAAGPWSRSSTPLTREE"
BASE COUNT          389 a          727 c          778 g          432 t
ORIGIN      
        1 ttcgtccgtg tcacgggatc tgcgctcgct ttgaaggaac atgagctccg gaacaatgcg
       61 ccgcttgtaa accacaaagg ggtgggttag cttgaatcaa tgagcctggg ccgccgcctt
      121 cgtcgcaggt atcaggtgaa aatcctgacc gtcctgaggt tcctgcccga tcgcagcggt
      181 gctccgcccg tgcctccgcc gtgggatccc ttcgagcggg agatcccgga ggatctgctc
      241 acctccccgg gcgaacttcg cgatccggtt gaagaagagc ggatcgcgag ggaggagccg
      301 ctgcacgagt tcttcagcgt tcacccggag gcaagcgagt tcatcgtcgc ccacggcagg
      361 ggctttctcc agtcgacctt caccaggctg gccaggggag tccaccggat ccgtcgagcg
      421 acccgctttc gtcccagccc gaaccggagc cccgcgagcg ctgccgagat gacctcgatc
      481 ctccgcctgg aggccgaacg catcggactg agccggatcg gcatagcgcc gaacgatccg
      541 aagtacacct tccccgagtc tgatgcgaaa cagctgccga acgtggtggt ctgtctggtc
      601 gagcaggact gggaattgac ccagatgatc cccagtgacg aggccgagaa gtcggccatg
      661 tacggctact ccgaagggat gaccagggca gcccagctgg cagagttcct caaacgaatg
      721 ggattcgacg cggcgcctca gggcttcccc ggtgacgcga tcacgattca ctacggggtg
      781 gcatccgggc tggggcaact ggggctgaac ggtcagcttc tcaccccgga ggccggctcc
      841 agatgccgca tcctgctgtt gactaccgac gccccgttga ctcatgacag tcccgtcgac
      901 tacggcctgc acaaggtctg tgatgaatgc aaggcctgcg tccggaactg tccggtcggg
      961 gcgatccccc agacccgcag gccctaccgg ggagtcgtca agtcgaagct gaacacggtt
     1021 cgctgcctgc cggtcgtcgc ccaggccagc ggctgcgccg tctgcatgaa ggtctgtccc
     1081 gtccagcgct acggcctcga agcgatcctc gaccatcgtg agcagaccgg cgagatcctc
     1141 ggaatcggct cggacgagct ggagggatac gactggccgc tcgatggcag gcattacggt
     1201 ccgggcgaga cccccaaggt tggagatgaa gtgttgaaca accccgacct ggtcttcgac
     1261 ttctcccgca aggagcctcc tccgggcatc gggccgatca ggttcgtcgg ctgacctgga
     1321 tcttcttcac gaacgggtag acgacgctct tcgcgatcat tcttccctcg tcaggggtgt
     1381 acttgatctt gaccagggcc cggccgctcc ccgacggttc aacttgcgca gcagcgggcc
     1441 cttcgggacg gccttgatca gataggtccc ggcctgatcg aagtgggcgc tggaaccctt
     1501 cactctcttg tagccgatca actccacctg accggcggcc gggatcttca ccctgatctt
     1561 gttgtatccg ttcttcggga acttgatcgc tccgagcagg cgcagcggac ggtcgacaac
     1621 cggcggcggg gtgggggtca cctcggtcgt cacgttggtc ggaacgaccg actgcgaggc
     1681 ggtgaagttg ttccggtccc cgtcgccggt gtaggacgcg gtgaccgggt aggtgcccgg
     1741 atccgggtac gagaccgtgc acgttgcggc cccgttcgcg aggtactccg cggggcagtt
     1801 gacggtggct gggtttcccg caccatcgtc gaaggagacg gtgccgccgt cgggcgccgg
     1861 cgtgatcgtc gcggtgtagg tcaccggctg gttctgctcg acgctgctcg ccgaggccgt
     1921 caattcgatc tcggtctgct ggagcagata ggcgcccatg tcacaggcga ttcccgcggc
     1981 aggcaatacg ccccgctggt cggccaggga gcaggcgttg gactccccgc tgaccggggt
     2041 ggggaagtcg taatcggtcg gaaccacggc cagcgccggg ttggccaggt cggttgccgg
     2101 atccgggctg ttcagcagcg cgaaggtcct gctctggccc ccgttgaagg cagccacatc
     2161 ggcgagatag gaatcgagca cttccccgta ggtggaggag ccgtaggcgg tggtgccggc
     2221 gtggcttccc gttccgggca tggtgggaga gatgcaggtg ccgtcgtcgt cgatgttgta
     2281 tccggcgtca gttggagttg ctccattgca ggagccgttg ggtgac
//
LOCUS       DAOTIP010000327         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54765_length_2321_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOTIP010000327 DAOTIP010000000
VERSION     DAOTIP010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54765_length_2321_cov_3.000000"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..734
                     /locus_tag="P5138_06800"
     CDS             102..734
                     /locus_tag="P5138_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRV60322.1"
                     /translation="MSQIKMVISDFGGVLTTPLVEAFASVQDRVGIPPKTFGEALRAL
                     NEREGTNPLFELECGRITEKEFQVQLADGLEPLLGHRLEIEDFGLLLFEALDPNPGMI
                     DLIREVRRDGIRTSLLTNNVKEWEVKWRSMMPIDELFETVVDSAFVGCRKPDPRIYNL
                     TLERVGLDPEECIFIDDMKINIDAANELGLHGVHFRETAQVRAEVHDLLA"
     gene            complement(817..2013)
                     /locus_tag="P5138_06805"
     CDS             complement(817..2013)
                     /locus_tag="P5138_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRV60323.1"
                     /translation="METELKPGAGRNLALATVGFALCFAAWGFLAPLAPSFKDPPLNL
                     SSLQTSIAIAIPVLLGSLLRIPLGILTDRIGGRKVFTGMLIYSAAAAALVGFAAGSSS
                     YAAILGAGFLLGAAGASFAVGIPFVARWYPPQKQGFALGIYGMGNIGTAVAAFSIPAI
                     RDATSEGVAGLVFAAVIGVYALIWMSMAREAPVEKAPPTRYREVLGAGWKLWRLALFY
                     FVTFGGFVAMALYLPKLLVDWYDLSLTDAGLRAAGFTLLATAIRPVGGMLSDRIGGNR
                     VLAVAFLGIGIDAVGLSWQASVPTMWLTTVFCLTMALFLGLGNGAVFKLVPLTFPNAT
                     GAATGIVGAVGGLGGFFPPLFLGIVKDATGDFVLAFVMLVAFAWLCAGLAFEGRVPDS
                     GTNGPD"
     gene            complement(2060..>2321)
                     /locus_tag="P5138_06810"
     CDS             complement(2060..>2321)
                     /locus_tag="P5138_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRV60324.1"
                     /translation="TIFQVHVIAVWFLYAAWPFSRLVHAFTFPINYPRRSPIIYRPRK
                     PQVAAAKSKSYGSGNLDYGPSIPSGPKPTGTTGGRSRAAQGE"
BASE COUNT          438 a          714 c          817 g          352 t
ORIGIN      
        1 gggggctcga cgatcaacac cgccctcttc cgctggtcgc tcgatggcca cctcggcctc
       61 ggccgttacg agatcacccg tcccgcggcg gtagcggtcg catgagccag atcaagatgg
      121 tcatctcgga tttcggcggg gtgctgacga ccccgctggt cgaggcattc gcttcggtgc
      181 aggatcgggt cgggatcccg cccaagacct tcggcgaggc gctgcgggcg ctgaacgaga
      241 gggagggcac caatccgctc ttcgagctcg agtgtggccg gatcaccgaa aaggaatttc
      301 aggtccagct ggcggatggc ctcgagccgt tgctcggcca ccggctggaa atcgaggact
      361 tcggcctgct gctcttcgaa gcccttgacc cgaacccggg catgatcgac ctgatccggg
      421 aggtccgccg ggacggcatc cgcacttcac ttctgaccaa caacgtcaag gagtgggaag
      481 tcaaatggcg ctcgatgatg ccgatagacg agctcttcga gaccgtggtc gactcggctt
      541 tcgtcggctg ccgcaagccg gatccgcgga tctacaacct gaccctggaa cgggtcggcc
      601 tcgacccgga ggaatgcatc ttcatcgacg acatgaagat caacatcgat gccgcgaacg
      661 aactcgggct ccacggggtt cacttccgcg aaaccgcaca ggtccgggcc gaagtgcacg
      721 acctgctggc gtaaacaggg tttcgcctgg ctttacttcg ggcggagccc ctcccgctcg
      781 gaggtcggga ggggcgccgt accgaagtgg ctcagactag tccgggccgt tcgtccccga
      841 gtcgggaacc cggccctcga aggccaggcc ggcacacagc caggcgaagg cgaccagcat
      901 cacgaaggcg agcacgaagt cacccgttgc gtccttcacg attcccagga acaggggcgg
      961 gaagaagccg ccgaggccac ccacggcgcc gacgatgccg gttgcggcac cggttgcgtt
     1021 cgggaaagtg agcggcacca gcttgaacac ggcgccgttc ccgagaccga ggaagagggc
     1081 catggtcagg cagaagacgg tggtcagcca catcgtcgga accgaggcct gccaggagag
     1141 gccgaccgcg tcgatgccga tcccgaggaa ggcgaccgcc agtacgcggt tgccaccgat
     1201 ccggtccgag agcatgccgc cgaccggcct gatcgcggtt gccagcaggg tgaagccggc
     1261 agcgcgcaac ccggcgtcgg tgagcgaaag gtcataccag tcgaccagca gcttcggcag
     1321 gtatagcgcc atggcgacga atccaccgaa ggtcacgaag taaaagagcg ccagtcgcca
     1381 gagtttccag ccggcgccga gcacctcgcg gtagcgggtc ggcggcgcct tttcgaccgg
     1441 tgcctcccgg gccatcgaca tccagatcag cgcgtagacg ccgatcactg cggcgaagac
     1501 gagtcccgcc acaccttcac tggtggcgtc gcggatcgcc ggaatcgaga aggcggcaac
     1561 cgcggtgccg atgttgccca tgccgtagat gccgagcgcg aagccctgct tctgcggcgg
     1621 gtaccagcgg gcgacgaacg gaatgccgac cgcgaaggat gctccggcgg cgccgagcag
     1681 gaagccggcg ccgagaatgg ccgcgtagga cgatgacccg gcagcgaatc cgaccagtgc
     1741 cgcggctgcg gccgagtaga tcagcatccc ggtgaagacc ttgcggccac cgatccggtc
     1801 ggtgaggatg ccgagcggga tgcggagcag cgagccgagc aggacgggga tggcgatcgc
     1861 gatcgaggtc tgcaggctgg aaaggttgag cggcggatcc ttgaacgacg gcgcgagcgg
     1921 ggcaaggaac ccccaggccg cgaagcagag cgcgaatcca accgtggcga gagccaggtt
     1981 gcgccctgca ccgggtttga gttcagtttc catcggattc tcctgttggt gtgttagtgg
     2041 aaagagggtt gagtcgcggc tactcgcctt gcgcggcccg cgaacgaccg ccggtcgttc
     2101 cagttggttt ggggccggag gggatgctgg gtccgtagtc gaggtttccg ctgccgtagc
     2161 tctttgactt ggcggctgcc acctgcggct tgcgcgggcg gtagatgatc gggctgcggc
     2221 gggggtagtt gatcgggaag gtgaaggcgt ggaccagccg gctgaacggc caggccgcgt
     2281 agaggaacca gacggcgatc acgtgaacct ggaagatggt g
//
LOCUS       DAOTIP010000328         2319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54829_length_2319_cov_3.073763, whole genome shotgun sequence.
ACCESSION   DAOTIP010000328 DAOTIP010000000
VERSION     DAOTIP010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54829_length_2319_cov_3.073763"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="P5138_06815"
     CDS             complement(<1..368)
                     /locus_tag="P5138_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRV60325.1"
                     /translation="MAKFTPDTIDRVRAAADMVEIVSAHTDLKRAGGGRYMGLCPFHE
                     ERSPSFSVNATEKLYHCFGCGVGGDLFEFVMSTGNLDFPDAVEMLADRYGVEITREQE
                     DPKAEKRRQERVRLTELMERA"
     gene            complement(415..1497)
                     /locus_tag="P5138_06820"
     CDS             complement(415..1497)
                     /locus_tag="P5138_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRV60326.1"
                     /translation="MNADDRQKAPEGPGDGQMAPERPGDGPDSDFERLTREREELLLG
                     PRAVRSYPSDRPEPEPDSQWRTPFQRDRDRIVHSKSFRRLMHKTQVFIAPEGDHYRTR
                     LTHTLEATGIARTVARALRLNEDLTEAIGLAHDLGHPPFGHIGEQALDEASRAHGGPG
                     FKHNENSLRVVDVLEREGRGLNLNQPVRDGILKHTGSVKSESLEGQIVRLVDRVAYIN
                     HDIDDALRAGILMAEDLPGDEISLLGPTGASRIDSLVKDIVEQSRDSDEIVQSDDMGK
                     AMLRLRKFMFESVYLGDEARRDHDRVHGVIRALFEHYLERIDEVPVLDPGADPIQRVT
                     DLLAGMTDRYCIAHYTAIYVPEGSRF"
     gene            complement(1494..2234)
                     /gene="recO"
                     /locus_tag="P5138_06825"
     CDS             complement(1494..2234)
                     /gene="recO"
                     /locus_tag="P5138_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRV60327.1"
                     /translation="MSRTFKTEAVVLRTIRYGEADRILHLYTREFGRLGAIAKGVRKP
                     RSRFGGRLEPFFRLALVLHRSRGDLHTVTSAETIEGFPELRASGRGINAAADACGGVL
                     RLLDGAEEPNEPAYNLLCRYLELVNADPEGPAASGAGMVAFRLKLALAAGFAPELSGC
                     ARCGANDHMTAFSGAAGGVVCPTCEAGSFPFSADAHTFMVQALGRPMAELPAGDAVAI
                     RQAGRAIGETLEHHAHVRLPRPEERLAA"
BASE COUNT          362 a          784 c          761 g          412 t
ORIGIN      
        1 gcccgttcca tcagttcggt cagcctgacc cgctcctgcc ggcgcttctc ggccttcgga
       61 tcctcctgtt cgcgggtgat ctccacgccg tagcggtcgg ccagcatctc gaccgcatcc
      121 ggaaagtcca ggttccctgt actcatcacg aactcgaaaa ggtcgccgcc gaccccgcag
      181 ccgaaacagt ggtagagctt ttccgttgcg ttgaccgaga aggacgggga acgctcttcg
      241 tgaaaggggc agagccccat gtaacggccc ccgccggccc ttttcaggtc ggtgtgggcg
      301 gaaacgatct cgaccatgtc cgcggcagcg cgaacccggt cgatcgtgtc tggagtgaac
      361 ttcgccatgc gggtccgagt ctagaagagc agaccccgcc cgactccccc tacctcagaa
      421 ccgtgaacct tcgggcacgt aaatcgcggt gtaatgcgcg atgcagtagc ggtcggtcat
      481 cccggccagg aggtcggtga ccctctggat cgggtcggcg ccgggatcga gaaccggaac
      541 ctcgtcgatc ctctcgaggt aatgctcgaa aagggcccgg atcacgccgt ggacccggtc
      601 atggtcgcgc cgggcctcgt cccccagata gacgctctcg aacatgaact tgcgcagccg
      661 gagcatcgcc ttgcccatgt cgtcgctctg aacgatctcg tcggagtccc gcgactgctc
      721 gactatgtcc ttgaccagcg aatcgatccg gctggcaccg gtcggcccga gcagggagat
      781 ctcgtcaccc ggcagatcct cggccatcag gatcccggcc cggagggcgt cgtcgatgtc
      841 gtggttgatg taggcgaccc ggtccaccag ccggactatc tgcccctcca gggactcgct
      901 cttgaccgat ccggtgtgct tgaggattcc gtcgcggacc ggctggttca ggttgagacc
      961 ccgtccctcc cgctccagta cgtcgacgac ccgaagcgag ttctcgttgt gcttgaagcc
     1021 cggcccgcca tgtgcccggc tggcctcgtc cagcgcctgc tctccgatat gtccgaaggg
     1081 cggatgtccg aggtcgtggg cgaggccgat cgcctcggtg aggtcttcgt tcagacgcag
     1141 cgcccgggca accgtgcggg cgatgccggt cgcttcgagg gtgtgagtga gccgggtccg
     1201 gtaatggtct ccctcggggg cgatgaagac ctgggtcttg tgcatgaggc gacggaagga
     1261 cttgctgtgg acaatccggt cccggtcccg ctggaatgga gtgcgccact ggctgtccgg
     1321 ttccggttcg ggccggtcgg agggatagga gcgcactgcc cgggggccga gcagcagctc
     1381 ttcccgttca cgggttagcc gctcgaagtc actgtccggc ccgtcgccgg gccgttccgg
     1441 agccatctgt ccgtcgccgg ggccttccgg agccttctgc cggtcgtccg cgttcacgcc
     1501 gccagccttt cctccggccg cggcaacctc acatgggcat ggtgttccag ggtttcgccg
     1561 atcgcccggc cggcctgccg gatcgccacg gcatctcctg cggggagctc cgccatcggc
     1621 cttccgagcg cctgcaccat gaaagtgtgg gcatcggccg agaagggaaa cgaacccgcc
     1681 tcgcaggtcg ggcagaccac cccgccggcg gcacccgaga aggcggtcat gtgatcgttg
     1741 gcgccacaac gggcgcagcc cgacaactcg ggcgcgaaac cggctgccag ggcgagcttg
     1801 agccggaaag caaccattcc cgcgccggag gcggccggcc cttcggggtc ggcgttgacg
     1861 agttccagat agcggcagag aaggttgtag gccggttcat tcggctcttc ggcgccgtcg
     1921 agcaatcgca gaacgcctcc gcaggcatcg gcggcggcgt tgatcccgcg gccgctggcc
     1981 cggagttccg gaaagccctc gatcgtttcg gccgaggtaa cggtgtgcag gtcgccacgg
     2041 ctccgatgga ggacgagcgc gagccggaag aacggctcca gcctccctcc gaaacgggac
     2101 cggggcttgc gcacgccttt ggcgatcgcc cccagccggc cgaactcgcg cgtgtagagg
     2161 tggaggatcc ggtcggcctc cccgtacctg atggtcctca gcaccaccgc ttctgtcttg
     2221 aaagtacgtg acatctcgag ccttaatggt gtcgcccgaa ccggacggcg cctgacgcta
     2281 ccttcgcctg ggcttgcgcc gcagcctctt ctggcaacc
//
LOCUS       DAOTIP010000329         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_54876_length_2317_cov_3.492485, whole genome shotgun sequence.
ACCESSION   DAOTIP010000329 DAOTIP010000000
VERSION     DAOTIP010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54876_length_2317_cov_3.492485"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="P5138_06830"
     CDS             complement(<1..327)
                     /locus_tag="P5138_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRV60328.1"
                     /translation="MSEGPAPLARLTAELSQLPGIGRRTAQRLAFHILRSDDSDARSL
                     AEAILEVKEKVGLCEVCFNLAEGPRCRICEDPSRDESLICVVEEPADVIPLERTHEYR
                     GLYHVLG"
     gene            complement(324..671)
                     /locus_tag="P5138_06835"
     CDS             complement(324..671)
                     /locus_tag="P5138_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRV60329.1"
                     /translation="MARKGMGGMPGGMDMNALLKQAQQMQNEMMQAQEALKDEIVEAS
                     AGGGMVKVKISGDLVIQDVSIDPDAVDPEDSEMLAEMVQAAINEAIRSAQQLAESKMP
                     QMPNMPGMPGLGG"
     gene            complement(671..>2317)
                     /gene="dnaX"
                     /locus_tag="P5138_06840"
     CDS             complement(671..>2317)
                     /gene="dnaX"
                     /locus_tag="P5138_06840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRV60330.1"
                     /translation="TLTPCGTCESCRSIAAGNSVDVIEMDAASNRSVDDIRELRDRVA
                     FAPTAGRWKVYILDEAHMLTKEAWNAFLKTLEEPPPNTVFILATTEAHKVMPTIADRC
                     QRFDFQRPSIEQLTEVLTRVAAAEQIAIDAPAISMISRSASGSFRDALGTLDQLVSYS
                     GNEVSLDDVLEVLGAADADLLFGVVDKVIQGDPAGVLTSVEQVARSGRDPARFARDLL
                     GHLRVLLVTRTTGQIPEAFAVTASDPDRLARQAKAIGPANLIRTIDELSAALGLIRDG
                     DEARLAVEVALLKAARPDFDPSSEGLARRLERLEQGTQPTPQSTVSESGGAAAAVGDP
                     VGRTSPGVPPGPPTATASAPDAEPTSSGPGSAPSAEPSKVESAGDTAVRPDEVRPVEP
                     PTSVASAEATSAEVSDGSSPVEPASAGPVPVDNAGPEPFDLERLEQVWPAILDSIGGS
                     GSGMVASYFEGTRPLNLVEGKLTIGFPADSSFNRRNADRPECRQQLAAAVTAVTGDQF
                     QIEYDSLEGGQAAADPESEVMDEEDFIARVKSEFNAEEVI"
BASE COUNT          367 a          754 c          793 g          403 t
ORIGIN      
        1 accgaggacg tggtagagac cgcgatattc gtgggtccgc tcgagcggga tcacgtcggc
       61 cggttcctcg accacgcaga tcagcgactc gtcccgagag gggtcctcgc agatccggca
      121 gcgcggacct tcggccaggt tgaagcagac ttcgcagagg ccgaccttct ccttcacctc
      181 gaggatcgct tcggcgaggc tgcgggcgtc ggagtcatcc gacctcagga tgtggaaagc
      241 gagacgctgt gcggtgcgtc ggccgatccc cggaagctgc gagagctcgg cggtgagacg
      301 agcgagtggt gcgggcccct cgctcatcca ccgagcccgg gcatgccggg catgttcggc
      361 atctggggca tcttggattc ggccagctgc tgggcggagc ggatcgcctc gttgatcgcc
      421 gcctggacca tctctgcgag catctccgaa tcctcggggt cgaccgcgtc ggggtcgatc
      481 gagacgtcct ggatgaccag gtcgccgctg atcttcacct tgaccatgcc cccgccggcg
      541 cttgcttcga cgatttcgtc cttgagcgcc tcctgggcct gcatcatctc gttctgcatc
      601 tgctgcgcct gcttgagcag cgcgttcatg tccattccgc cgggcatgcc gcccattccc
      661 ttgcgtgcca tcagatgacc tcctcagcgt tgaattcgga tttgacccgg gcgatgaaat
      721 cttcctcgtc catgacttcg gactccgggt ccgcagccgc ttggccgccc tcaagactgt
      781 cgtattcgat ctggaactga tctccggtca ccgccgtcac cgccgcggcc agctgctgcc
      841 ggcattccgg gcgatccgcg ttacgccggt tgaagctgga gtcggccggg aatccgatcg
      901 tcagctttcc ttcgacgaga ttcaacggcc gggtaccctc gaaatacgag gcgaccatgc
      961 cggagccgga gccaccgatc gaatccagga tcgccggcca gacctgctcc agccgctcca
     1021 gatcaaacgg ttcgggaccg gcattgtcca ccgggacggg accagccgaa gcgggctcca
     1081 cgggggaact cccgtccgaa acttcggccg aagtcgcctc agcggaggcg accgacgttg
     1141 gcggttccac cggccgcact tcgtctggtc ttaccgcggt atcccccgcg gactccacct
     1201 tcgaaggctc cgcggaaggt gccgaccccg ggccagagga agtgggctct gcatccgggg
     1261 ctgaagcggt cgccgttggg ggtcccggcg gaactcccgg cgaagtccgg ccgacgggat
     1321 ccccaacggc ggccgctgct ccgccagatt cggagacagt tgactgagga gttggctgag
     1381 tgccctgttc gaggcgttcc aggcggcggg cgagaccttc gcttgaggga tcgaagtcgg
     1441 gccgggcggc cttgagcagc gccacttcga ccgcgaggcg ggcctcgtcg ccgtcgcgga
     1501 tcaggccgag cgcggccgac agttcgtcga tcgtgcggat caggttggcc ggaccgatcg
     1561 ccttcgcctg gcgggccagg cggtccgggt cggaagccgt gaccgcgaaa gcttcgggta
     1621 tctggccggt ggtccgggtg accagcagca cccgcaagtg gccgagcagg tcccgggcga
     1681 aacgggccgg gtcgcggccg gaacgggcga cctgttcgac cgaagtgagg actccggcgg
     1741 gatcgccctg aatcaccttg tcgaccacgc cgaagagcaa gtcggcatcg gcggcaccga
     1801 gtacctcgag cacgtcgtcg agcgacactt cgttgccgct gtaggagacg agctggtcaa
     1861 gggtgccgag cgcgtcgcgg aagctgcccg aagcggaacg gctgatcatc gagatcgcgg
     1921 gagcatcaat cgctatctgc tccgccgccg ccacccgggt tagcacctcg gtcagctgct
     1981 cgatcgaggg acgctggaag tcgaagcgct ggcagcgatc ggcaatggtc ggcatcacct
     2041 tgtgggcctc ggtggtcgcg aggatgaaga ccgtgttcgg cgggggttcc tccagcgtct
     2101 tgaggaaggc gttccaggcc tccttggtca gcatgtgggc ctcgtcgagg atgtagacct
     2161 tccagcggcc ggcggtcggt gcgaaggcca cccggtcacg gagttcgcgg atgtcatcca
     2221 ccgaccgatt cgaggcggcg tccatctcga tcacgtcgac cgagttgccg gcggcgatcg
     2281 agcggcagga ctcacaggtc ccgcaggggg tcagggt
//
LOCUS       DAOTIP010000330         2308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_55169_length_2308_cov_3.568131, whole genome shotgun sequence.
ACCESSION   DAOTIP010000330 DAOTIP010000000
VERSION     DAOTIP010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55169_length_2308_cov_3.568131"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="P5138_06845"
     CDS             <1..465
                     /locus_tag="P5138_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRV60331.1"
                     /translation="SHPGPDPPAEADGQLTDEELRPLLVLVADCLPVALLGERGLAML
                     HCGWRGLAAGIINAATAEIGGVAAAIGPGIGPCCFEVGPEVEESFGNLGPDLMQGRNL
                     DLPEVTRRLLARSGVEAVESAGICTFCDERFFSHRRDQGRTGRQAGIAWLNG"
     gene            600..1163
                     /gene="sepF"
                     /locus_tag="P5138_06850"
     CDS             600..1163
                     /gene="sepF"
                     /locus_tag="P5138_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRV60332.1"
                     /translation="MALRDSWHRTLVYFGLAEEDDHHEYDDYEDDYLEEDEYESRRGR
                     RNREANVRRLPTSRRSRHDEIDDIFADDDPMPAPSRGRSRTLRSVGSDNGDDVQVHLV
                     IPRNFNDAQQVADQFKRKVPVILNLQTTDHELSKRLIDFASGLTYALDGGMQRIAEKV
                     FLLTPRNVEVSAEEKARLIDKGFFNQS"
     gene            1193..1966
                     /gene="proC"
                     /locus_tag="P5138_06855"
     CDS             1193..1966
                     /gene="proC"
                     /locus_tag="P5138_06855"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRV60333.1"
                     /translation="MIVGFIGSGNMAAAMARGWARAERNSPTEMLFTDSGSGRATALA
                     KEVGGRAVQGNDDLVRQSDFVILAVKPASLETVAKELPAPRAVLSLLGATPLEKLVSE
                     FPDSTVIRLMPNLGVEVNRGVICMAEPEGIDQEIVTESKQLLESIARVHELPDESMDV
                     ATAVMGCSPAYFAVVAEALRSAGTHGGLGADVAIRLVSESLAGTAELLKHRTPFEIQT
                     AVASPGGSTEAGLEALAAAGGADAFRAAYEASIARMRGD"
     gene            1970..2275
                     /locus_tag="P5138_06860"
     CDS             1970..2275
                     /locus_tag="P5138_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRV60334.1"
                     /translation="MEAILAVTRGDVAAYVNGLFTVYIILVFLRVLMSWIPRLPENSV
                     VQAVAGFVRETTDPYLNLFRRFLPPVGGGGFALDLSPVVGIFLLLIGQQIVVSLVRG"
BASE COUNT          412 a          733 c          752 g          411 t
ORIGIN      
        1 tctcatccgg gccccgaccc gccagccgag gctgacggcc agctgaccga cgaagagttg
       61 aggccgctgc tggtcctggt ggcggactgc ctgccggttg ccttgctggg cgaacgcggg
      121 ctggcgatgc ttcattgcgg ctggcggggc ctggccgccg ggatcatcaa tgccgccacc
      181 gcggagatcg gcggagtcgc ggcggcgatc ggtcccggaa tcggcccctg ctgcttcgag
      241 gtcggcccgg aagtggagga gtcttttgga aacctgggcc cggacctgat gcagggcagg
      301 aacctcgacc tcccggaggt gacccggcgg ctgcttgcca ggtccggggt ggaggcggtc
      361 gaatcggccg gaatctgcac tttctgcgac gaacggttct tctcgcatcg ccgggatcag
      421 ggcagaaccg gccgtcaggc cggcatcgcc tggctgaacg gttgaagccg gttccggctg
      481 catcctcgat gccgaaagcg tgattttcgc tgcaaagggg gtaattcggt gataattgcc
      541 ccgacccgac ccccgatcag aaggaatcgg cctccccgga ccgaaaacac cgcataccta
      601 tggcactacg tgattcctgg caccgcactc tcgtttactt cggcctcgcc gaggaagacg
      661 accatcacga atacgacgat tacgaagacg actacctcga agaagacgaa tacgaaagtc
      721 gtcggggtcg ccgcaatcgt gaagcgaacg tccggcgtct gccgacttcg cgccgttccc
      781 gccacgacga gatcgacgac atcttcgccg acgacgatcc gatgccggcg ccgagccgcg
      841 gtcgttcccg gaccctgcga tcggttggca gcgacaacgg tgacgacgtc caggtgcatc
      901 tggtcattcc ccgcaacttc aacgatgcgc agcaggtagc cgaccagttc aagcgcaagg
      961 ttccggtcat cctcaacctc cagaccaccg accacgagct ttcgaaacgg ctgatcgact
     1021 tcgcatcggg gctgacctac gctctggacg gtggcatgca gcggatcgcc gagaaggttt
     1081 tcctgctcac accgcgcaac gtcgaggttt ccgccgaaga aaaggcccgt cttatcgaca
     1141 agggcttctt caaccagtcc tgacccgcgg ctgaatccat aaagtgtccg gcatgattgt
     1201 cgggttcata ggttccggaa acatggcggc ggcgatggcc cgcggctggg ccagggcgga
     1261 gcggaactct ccgaccgaga tgctcttcac cgattcaggt tcgggacgtg ccacggctct
     1321 cgccaaggaa gtcggcgggc gcgcagtcca gggaaacgac gacctggtgc gacagtcgga
     1381 cttcgtaatc cttgcggtca agccggcctc actggagacg gtggcgaaag agctgcctgc
     1441 cccgcgggcc gttctgtccc tgctcggggc cactcccctc gagaaactgg tctcggaatt
     1501 ccccgacagc accgtgatcc ggcttatgcc gaaccttggc gtcgaggtca accggggcgt
     1561 catctgcatg gcggagccgg aaggcatcga ccaggaaata gtcaccgaga gcaagcagct
     1621 gctcgagtcg atcgcccggg ttcatgagct gcccgatgag agcatggacg tcgcaaccgc
     1681 cgtcatgggg tgttcgcccg catactttgc ggtcgtcgcc gaggctctcc ggagtgcggg
     1741 tacccacggt ggcctggggg cggatgtggc gatccggctg gtcagcgaat cgctggccgg
     1801 gaccgcggaa ctgctcaagc accgcacacc cttcgagatc cagacggcag tcgcttcccc
     1861 ggggggaagc accgaagccg gactcgaagc actcgccgcg gccggaggtg ccgacgcatt
     1921 cagggccgcc tacgaggctt cgatcgcacg gatgcgcggg gactgacccg tggaagcgat
     1981 actcgccgtg accaggggcg acgtggcggc ctatgtgaac ggcctcttca ccgtctacat
     2041 catcctggtc ttcctccggg tgctgatgag ctggattcca cggctgcccg agaactccgt
     2101 ggtccaggcc gtggccggat tcgtgaggga aacgaccgat ccctacctga acctgttccg
     2161 ccgcttcctg cctccggtcg gaggaggggg ctttgctctt gacctgagcc cggtggtggg
     2221 aatcttcctg ctgctgatcg gacagcagat cgtcgtctct ctcgttcggg gctgagccgg
     2281 tttgaggggc tacaggcatc tgatctgg
//
LOCUS       DAOTIP010000331         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_55457_length_2300_cov_2.863252, whole genome shotgun sequence.
ACCESSION   DAOTIP010000331 DAOTIP010000000
VERSION     DAOTIP010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55457_length_2300_cov_2.863252"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..>2300)
                     /locus_tag="P5138_06865"
     CDS             complement(105..>2300)
                     /locus_tag="P5138_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRV60335.1"
                     /translation="MTVLVPTVTLDKTTGLPQFPDTGDAQTETPFPWRILITNPNTGS
                     SLLGVDLTDTLPPNWTYVPNSAQITGTGTLTPGGQVNPTITQNPTGDVLVWSNIANLV
                     GVQNAVVSFNAIPEPEAAIDPGLTNPHVNEAEAIGEDTSGATASGDGPYTDDDDATAT
                     LETPVTDIQIVKTADDPAPTAGTNTTWTLVVKNNGPKVSPDVEVSDVLPAGLTYVSAT
                     SSQGTCAENPAGTMHCQIGQMNVDDEVTIHLTTAVGAGTVGQTLTNPADVDDPYIDDS
                     NPANNHSEDDVIPTESADVGIGKSIDEPLFAGQTGSYKLTVTNHGPSVARGVTVTDTL
                     PENLTYAGATTPVGSCSGSGQDFSCDLGDLDPGQTVVIDVSVNVLEGGEYTNCADVAS
                     PTPDPDGDNNHSCTTNPAENTDLAIEKTGPPYFPEGKNRNYTLLVTNVGDQPTGGTTT
                     VTDRIPDVLRPEAAYGNGWSCAISGQKVTCTRSDVLEPGDSFPLITIRVVARENLLFR
                     PIGNTGHVHLPGDPNPDNDQDTVRTKKGAICEGGSLALNPAFVWVGQKTIVKATLRTR
                     AGAPAIGLPVRIKGNGKGGASGKLRILTTNGKGKVHFTARATSTEARWTVSVPQSHQK
                     AVLAPKRQETCRAMNVTPRTIDINKTTVLTVRLRTPEGRPAIGITVVARGQGTGDSAR
                     TNGKGMARLRVRPNGQGYLVVTAPNATRCRFRIGASDLDAAGAGNQLTG"
BASE COUNT          308 a          720 c          819 g          453 t
ORIGIN      
        1 gaaggcttcg gggcgcgctg cgctggccgc tgcggagcca tcccagacga gcagcagcga
       61 aagagaaacc agcaccccgg ttactacacc ccgccggacc atggctaacc ggtcagctgg
      121 ttaccggcgc cggcagcgtc caggtcactg gccccgatcc ggaaccggca acgcgtggca
      181 ttgggcgcgg taaccacgag gtaaccctga ccgttgggac ggaccctgag gcgggccatg
      241 cccttgccgt tggtccgagc cgagtcaccc gtgccctgac ccctggcgac cacggtgatg
      301 ccgatggccg gacggccttc gggagtccgc agacggacgg tcagcaccgt ggtcttgttg
      361 atgtcgatcg tccgcggggt cacgttcatc gcccggcagg tttcctggcg cttcggtgcg
      421 agcaccgcct tctggtgact ttgcggcacg ctcaccgtcc agcgggcctc cgtactggtc
      481 gcccgggcgg tgaagtggac cttgcccttg ccgttagtgg tcaggatccg cagcttgccg
      541 ctggcgccac ccttgccgtt gcccttgatc cggaccggga gcccgatcgc cggagctccg
      601 gctctcgtcc gcagggtcgc cttgacgatc gtcttctggc cgacccagac gaaggccggg
      661 ttcagggcca gagacccgcc ctcgcagatc gcgcccttct tcgtgcggac ggtgtcctga
      721 tcattgtccg ggttcgggtc tcccggcagg tgcacgtggc cggtgttgcc gatcggccgg
      781 aagagcaggt tttcgcgggc aaccaccctg atcgtgatca gggggaatga atcaccgggc
      841 tcgagcacgt cggagcgggt gcaggtgacc ttctggccgc tgatcgcgca gctccagccg
      901 ttgccgtagg cggcctccgg cctgagcaca tcgggaatgc ggtcggtcac ggtggtggtg
      961 cccccggtcg gctggtcacc gacgttggtc accagcaggg tgtagttacg gttcttgccc
     1021 tcggggaagt acggggggcc ggtcttctcg atcgccaggt cggtgttttc cgccgggttg
     1081 gtggtgcagg aatggttgtt gtcgccgtcc gggtccgggg tcgggctggc cacgtcggcg
     1141 cagttcgtgt actccccgcc ttcgagcacg ttcaccgaca cgtcgatcac cacggtctgg
     1201 ccggggtcga gatcaccgag atcgcaggag aagtcctgtc cggaaccgct gcagctgccg
     1261 accggggtcg tggcgccggc ataggtcagg ttctccggca gcgtgtcggt gacggtcact
     1321 ccgcgggcga cgctggggcc gtggttggtg acggtcagtt tgtagctgcc ggtctggccg
     1381 gcgaagagcg gctcgtcgat cgacttcccg atcccgacgt cggccgactc ggtcgggatc
     1441 acgtcgtctt ctgaatggtt gttggccggg ttcgaatcgt cgatgtaggg gtcgtcgaca
     1501 tcggccgggt tggtcagggt ctggccgacc gtgccggccc cgaccgccgt ggtcaggtgg
     1561 atcgtcactt cgtcatcgac gttcatctgc ccgatctggc aatgcatggt tcccgccggg
     1621 ttttccgcac aggtcccctg ggaggaagtg gcgctcacgt aggtgagccc ggccggcagg
     1681 acgtcactta cctcgacgtc aggcgagacc ttgggcccgt tgttcttcac cacgagggtc
     1741 caggtcgtgt tcgtgcccgc cgtcggggcc gggtcatcgg cggtcttgac gatctggatg
     1801 tcggtgaccg gagtttcgag ggtcgccgtg gcgtcatcgt cgtccgtgta tggcccgtct
     1861 ccggaggcgg tagcgccgga agtgtcttcc ccgattgcct cggcctcgtt cacatgcggg
     1921 ttggtcagcc ccggatcgat cgccgcttcc ggctccggga tcgcgttgaa ggagacaacg
     1981 gcgttctgga ctccgaccag attcgcgatg ttcgaccaga ccaggacgtc accggtcggg
     2041 ttctgggtga tcgtcggatt gacctggccg cccggggtca gggtgccggt gccggtgatc
     2101 tgggccgagt tcggcacata ggtccagttc ggcggcaggg tgtcggtcag atcgacgccg
     2161 agcaggctgc tgcccgtgtt cggattggtg atcaggatcc gccacgggaa gggcgtctcg
     2221 gtctgggcgt caccggtgtc ggggaactgc ggcagcccgg tcgtcttgtc gagggtaacg
     2281 gtcgggacca gcaccgtcat
//
LOCUS       DAOTIP010000332         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_55528_length_2298_cov_3.041462, whole genome shotgun sequence.
ACCESSION   DAOTIP010000332 DAOTIP010000000
VERSION     DAOTIP010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55528_length_2298_cov_3.041462"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="P5138_06870"
     CDS             <1..718
                     /locus_tag="P5138_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRV60336.1"
                     /translation="EREIGKLMRQAATRIASGEADAPVRVDEEFLRDALGKQKFWQES
                     VERTAIPGVSTGLAVTGTGGDVLFIEATAMDGEKGMVLTGQLGDVMKESARIALSYVR
                     SHREELGVDAEAFDREFHLHVPAGAIPKDGPSAGTAMTTALVSLLTGRPVKHTVGMTG
                     EVTLQGRVLPIGGLKQKVLAAHAAGLTEVILPFRNKADLDDVPEEVREAMNFHPVSSV
                     EEVIDLALEPAGSIADVAPM"
     gene            744..1010
                     /locus_tag="P5138_06875"
     CDS             744..1010
                     /locus_tag="P5138_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV60337.1"
                     /translation="MRETAVVLERRWTMATIYACSSGATRFNEFRQSLPEVSPTTLSQ
                     RLEQLEEAGIVERHTVAGRPPHTEYALTDRGRQIALAVAALLDS"
     gene            1034..1837
                     /gene="ppk2"
                     /locus_tag="P5138_06880"
     CDS             1034..1837
                     /gene="ppk2"
                     /locus_tag="P5138_06880"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRV60338.1"
                     /translation="MSKSGKPKKLNKKEYEEELLRLQGELVQLQEWIIHQGLKVIVVF
                     EGRDTAGKGGVIKRITERTSPRVIRTVALGTPSDREKTQWYFQRYVAHFPAGGEMVLF
                     DRSWYNRAGVERVMGFCTDDEYQEFMRTTPRFERIIQRSGIILIKYWLSISDEEQERR
                     FKKRIKDPKKRWKLSPMDIEARSRWVDYAEAKDEMFAYTDTKDTPWWVVEADDKRTAR
                     LNLISHLLSMIPYEHVPSEEIDLPPRQDRSYVRPPMDTQNFVPTKFIVE"
     gene            1889..2290
                     /locus_tag="P5138_06885"
     CDS             1889..2290
                     /locus_tag="P5138_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRV60339.1"
                     /translation="MKLFAVTAIAFAVLGAAGCSGRDSGSGEDEAQITKVAQLYAQTA
                     EDHDYAATCRLFTPDSLARIKQLKVFGSCEHLMKAGLGALSKEEIQKLDDLTDLQVDG
                     DTATAQARGETINFEKVNGEWKLAIDTENES"
BASE COUNT          475 a          711 c          751 g          361 t
ORIGIN      
        1 cgaacgcgag atcggcaaac tcatgcgcca ggccgccacc cggatcgcct cgggagaagc
       61 cgacgcgccg gtgagggtcg acgaggagtt cctgcgcgac gcactgggca agcagaagtt
      121 ctggcaggag tcggtcgagc ggaccgccat ccccggtgtt tcgaccggcc tggccgtgac
      181 cggcaccggc ggagacgtcc tcttcatcga ggcgaccgca atggacggtg aaaagggcat
      241 ggtcctgacc ggccagctcg gtgacgtaat gaaggagtcg gcgcggatcg ctctctctta
      301 cgtccgctcg catcgcgagg agctgggtgt cgatgccgag gccttcgacc gcgagttcca
      361 ccttcatgtc ccggccggcg cgatccccaa ggacgggccg agcgccggca ccgcgatgac
      421 caccgccctg gtgtccctgc ttaccggccg acccgtcaag cacactgtgg gcatgaccgg
      481 tgaggtaacg ctgcagggca gggtgctgcc gatcggcggg ctcaagcaga aggtgcttgc
      541 cgcccacgcg gccggactga ccgaggtgat cctgcccttc cgcaacaagg ccgatctgga
      601 cgatgttccg gaagaggtgc gcgaggcgat gaacttccat ccggtctcca gcgtcgaaga
      661 ggtcatcgac ctggccctcg agccggccgg gagcatcgcg gatgtcgcac cgatgtaaca
      721 gctgccagcc ggtccccgcc gggatgcggg agaccgcggt ggtactcgag cgccgctgga
      781 ccatggcgac gatttacgcc tgctccagcg gtgccacccg cttcaacgag ttccggcagt
      841 cgctgccgga ggtttcgccg accacccttt cccagcgcct cgaacagctt gaggaggcgg
      901 gaatcgttga acgtcacact gtcgcgggac ggccgcctca caccgagtac gccctgaccg
      961 accgcggccg ccaaatcgcg ctcgcggttg cggcgctgct cgattcctga gccgggccga
     1021 ataagctccc cgaatgtcga agtccgggaa acctaagaag ctgaacaaga aggagtacga
     1081 ggaggaactt ctgcggcttc agggagaact cgtccagctc caggaatgga tcatccacca
     1141 gggactgaag gtcatcgtcg ttttcgaggg cagggacacc gccggcaagg gcggcgtgat
     1201 caagcggatc accgaacgga ccagtccccg cgtgatccgt accgtggcgc tcggcacccc
     1261 ttccgaccgg gaaaagacac agtggtactt ccagcgctac gtcgcccatt ttcccgccgg
     1321 cggcgagatg gtcctcttcg accgcagctg gtacaaccgc gccggcgtcg agcgggtaat
     1381 ggggttctgc accgatgacg aataccagga gttcatgcgc actacgcccc ggttcgagcg
     1441 catcatccag cgctcgggga tcatcctgat caagtactgg ctctcgatct ctgacgagga
     1501 acaggagcgg agattcaaga agcggatcaa ggacccgaag aaacggtgga agctgagtcc
     1561 gatggacatc gaggcccggt ccaggtgggt cgactatgca gaggccaagg acgaaatgtt
     1621 cgcctacaca gacaccaagg acacgccgtg gtgggtggta gaagccgacg acaagcggac
     1681 cgcccggctg aacctgatca gccacctgct cagcatgatt ccctacgagc atgtccccag
     1741 cgaagagatc gaccttccgc cgaggcagga ccgctcctat gtccggccgc cgatggacac
     1801 ccagaacttc gtgccgacga aattcattgt cgagtagcac cgaagccggg ataggcttgt
     1861 cgggccaggt ccgtcgaaag ggagaagcat gaagctgttt gctgttaccg caattgcatt
     1921 cgccgttctg ggagcagccg gctgtagcgg gagagattcg ggctccggcg aagacgaggc
     1981 gcagatcacc aaggtcgccc agctttatgc gcagactgcc gaagaccatg actacgcggc
     2041 gacatgccgt ctgttcacac cggactcgct ggccaggatc aagcaactca aggtgttcgg
     2101 gagctgcgag cacctgatga aggcggggct cggggcgctt tcgaaggagg agatccagaa
     2161 gctggacgac ctgaccgacc ttcaggtgga tggcgatacg gcaacagccc aggcccgggg
     2221 cgagacgatc aacttcgaga aggtcaacgg cgaatggaag ctggccatcg acacggagaa
     2281 cgagtcttga ccggtaag
//
LOCUS       DAOTIP010000333         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_55614_length_2295_cov_3.227232, whole genome shotgun sequence.
ACCESSION   DAOTIP010000333 DAOTIP010000000
VERSION     DAOTIP010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55614_length_2295_cov_3.227232"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /locus_tag="P5138_06890"
     CDS             <1..1972
                     /locus_tag="P5138_06890"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRV60340.1"
                     /translation="IVLLATIGTGMLWYERSRPPSQIVALVAVLAALAVAGRIVLAPI
                     PNVVATTDIVIIAGYVLGPAPGFAVGALGALVSNFWLGQGVWTPWQMVAWGMCGIGGA
                     VFFRLSRGRINRWTLAAVCGLAGLVFGAWMNLETMVSFGGEMSLDRYLALEVRAIPFD
                     IAHMTGNIVFALAAGPAMVAALTRFRQRFQWRRAEGETVGPADGSIRPAGAAGVTMVA
                     LAALVFAFAGSPAGKAEAAYTGQANRAATWLRGQQNSDGGFPSIPGSSSSVNITARAM
                     FALAATGTNPLDVKTNATPYGYLVNHRQEITDASDIALTILAMKTVGQNPRDFKGRNL
                     IEALRNRRGNGSFGNDVNVTAFAVLAFRSVQASSDAQYSLKWLYKAQNENGGWGIAKD
                     ANSDSDSTGTALMAITGEKAANRAIGYLDGIQKKSGGFGNSGNVNSQSTGLVMQGLVA
                     QGRGVNYLKKNGKTAADYLLSMQQEDGSIYYAKDNDVTRVWVTADGTTALAGKPLPIA
                     APAREDNSDTGNNSGGATPDSDSGTSGSTSTGGTTIPAGSLAGGSTGTASTGTGGSSG
                     VIDSGNSANQGNGANGGQNQGTGDSSVTPSTATPPTSTTFPVVPEAPSDAVLAASEAG
                     PQPSPLVAILIALGVGGGLCGGTILLGRRFRW"
     gene            1966..>2295
                     /locus_tag="P5138_06895"
     CDS             1966..>2295
                     /locus_tag="P5138_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60341.1"
                     /translation="MVGRDQSLEEIAGSHFEVVVIGGGITGAGVALDAASRGYSVALL
                     EGRDYGIGTSSRSSKMVHGGLRYLENFDVGLVREALLERQLLVELAPHLVYPTPFVVP
                     TFGEDKPD"
BASE COUNT          387 a          768 c          771 g          369 t
ORIGIN      
        1 gatcgtgctg ctggccacga tcgggaccgg aatgctctgg tacgagcgct cgaggccgcc
       61 gtcgcagatc gtcgccctgg tcgcggtgct ggccgccctc gccgtggccg gacgaatcgt
      121 gctcgccccg atcccgaacg tggtggcgac caccgacatc gtgatcatcg cgggctatgt
      181 gctcggaccg gcacccgggt tcgcggtcgg cgccctgggg gcccttgttt cgaacttctg
      241 gctcgggcag ggagtgtgga ccccgtggca gatggtggcc tggggaatgt gcgggatcgg
      301 cggtgccgtt ttcttccggc tgagccgcgg ccggatcaac cgctggacgc tggccgcggt
      361 ctgtggcctt gccggtctcg tcttcggcgc ctggatgaat cttgagacga tggtcagttt
      421 cgggggggag atgtcgctcg accggtacct tgcactcgag gtacgagcca ttcccttcga
      481 catcgcgcac atgaccggaa acatcgtctt cgccctggcc gccggcccgg cgatggtggc
      541 tgccctgacc cgcttcaggc agcgtttcca gtggcgccgg gctgaagggg agacggtcgg
      601 cccggccgat ggctcaatcc gcccggccgg tgcggccgga gtgacgatgg tcgctctcgc
      661 ggcgctcgtc ttcgcctttg ccggttcccc ggccggcaag gccgaggccg cctacacggg
      721 gcaggcgaac agggcggcga catggctccg tggccagcag aattcagacg ggggctttcc
      781 ctcgatcccc ggctccagct cctcggtcaa catcaccgcc cgggccatgt tcgccctggc
      841 cgcgaccgga accaatccgc tcgacgtgaa gaccaacgcg accccttacg gatacctggt
      901 caaccaccgc caggagataa ccgatgcgag tgacatcgcc ctgacgatcc tcgcgatgaa
      961 gacggtcggc cagaaccccc gtgacttcaa gggccgcaac ctgatcgagg ccctgcgaaa
     1021 tcgccgtggc aacggttcct tcggcaacga cgtgaacgtg accgccttcg cggtgctcgc
     1081 cttccgctcg gtccaggcga gcagtgacgc ccagtactcg ctcaagtggc tctacaaggc
     1141 ccagaacgaa aacgggggct ggggaatagc aaaggacgcg aacagtgact ccgattcaac
     1201 cggcaccgcg ctgatggcga tcaccggaga aaaagctgcc aaccgggcaa tcggatacct
     1261 ggacgggatc cagaagaaat ccggtggctt cggcaacagc ggcaacgtca actcccagtc
     1321 gaccgggctc gtgatgcagg gcctggtcgc tcagggcagg ggcgtcaact acctgaagaa
     1381 aaacggcaag acggccgccg actacctgct ctcgatgcag caggaggacg gcagcatcta
     1441 ctacgcgaag gacaatgacg tgacccgggt ctgggtcacc gcggacggaa ccacggccct
     1501 cgccgggaag ccgctcccga ttgccgcacc ggcgcgggaa gacaactcgg acactggcaa
     1561 caactccggc ggtgccaccc cggattccga cagcggcacc tccggcagca cctcgaccgg
     1621 gggcacgaca attccggcgg gatctctggc aggcgggtcg actggcaccg cctcgaccgg
     1681 aacgggggga agctccgggg tgattgacag cggcaactcc gccaaccagg gcaacggcgc
     1741 aaacggcggc cagaaccaag gcacgggcga ttcgtcggtt accccgtcaa ccgccacgcc
     1801 gccgaccagc acgacctttc ccgtggtccc cgaagctccg agcgacgcgg tcctcgccgc
     1861 atctgaagcc ggaccccagc cctcgccgct ggttgcgatc ctgatcgcgc tcggggttgg
     1921 cggcggtctc tgcggaggca cgatcctgct tggccggcgg ttccgttggt aggtcgcgac
     1981 cagtcgctcg aagagatcgc cggatcgcac ttcgaggtgg tggtgatcgg cggcgggatc
     2041 accggggccg gcgtcgcgct ggacgccgcc tcgcgcggct actcggtggc gctgctggag
     2101 ggccgtgact acgggatcgg cacctcctcg cgctcttcga agatggtcca cggaggtctc
     2161 cgctacctgg agaatttcga tgtgggtctg gttcgcgagg cgctgctgga acggcagctt
     2221 ctggtcgagc tggccccgca cctggtctat ccgaccccct tcgtcgtgcc gaccttcggt
     2281 gaagacaagc cggat
//
LOCUS       DAOTIP010000334         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_55735_length_2291_cov_2.788014, whole genome shotgun sequence.
ACCESSION   DAOTIP010000334 DAOTIP010000000
VERSION     DAOTIP010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55735_length_2291_cov_2.788014"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="P5138_06900"
     CDS             complement(<1..769)
                     /locus_tag="P5138_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60342.1"
                     /translation="MSLLAVPNVSEGRDFARLDRLEASLGEEVTLLDRHADVDHHRAV
                     FTLAGPAETLSTALTGLAGTSTVEIDMTAWDGIHPAIGALDVCPVVWLSEGRRETAAE
                     VATEVGQAIGAMGVPVFLYGDLATDADRRERAYFRAGGLDSLWQRMTSGGLKPDFGPG
                     QPHPTAGATLVTARAPIAAFNLELGTDDPRVAREIASVVREGPEEGIPGVRAMGLALS
                     TGRAQVSTNVHDPIGTPLGEVVQASPIARTPGIPSSGP"
     gene            complement(766..1713)
                     /locus_tag="P5138_06905"
     CDS             complement(766..1713)
                     /locus_tag="P5138_06905"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRV60343.1"
                     /translation="MSTELDQAVATVVRECLGVRPNEEVLVVCNPATRELGDALRSEA
                     ESAGAEAVLAVMTERASHAGEPPATVAEAMVAADVLLAPTVQSLSHTASRKRATESGT
                     RTATLPGATVEMLARVMSDDMEKLRHRGHRVAELLDRGTEARLTCDHGSDLTLGLEGR
                     KAIPDAGELTATGAFGNLPCGEGFIAPATAEGVLVVDGSIAGVGLVSGPVRLTIEGGH
                     LVDATGEEGEKLMELLTVHGPDGTNVAELGIGTNEKAILTGNILEDEEIIGTAHVAFG
                     ASAAIGGNVQVPVHLDVVSMRPEVTIDDTPVVLEGRLLV"
     gene            1828..2211
                     /locus_tag="P5138_06910"
     CDS             1828..2211
                     /locus_tag="P5138_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV60344.1"
                     /translation="MGLSNSKIAAVAAVTDMDRAKEFFEETLALEPMGGGAEGIEVMY
                     ACGDGTGLLVYPSPDHAGKGTATVAAWEVDDFDAETSALKERGISFEIYPGFDQDENG
                     VLDMGGARVVWFKDPDGNTFAVSGS"
BASE COUNT          362 a          803 c          748 g          378 t
ORIGIN      
        1 agggtccgga ggagggaatc ccgggcgtcc gggcgatggg actggcctgg acgacctcgc
       61 cgagcggggt gccgatcggg tcatggacat tggtcgaaac ctgtgccctg ccggtcgaaa
      121 gcgccagtcc catcgcccgg acgcccggga ttccctcctc cggaccctcc cttactaccg
      181 aggcgatctc tcttgccacc cgcggatcat ccgtcccgag ttcaaggttg aaggcggcga
      241 tcggggcgcg ggcggtgacc agggtggcgc cggctgtcgg atggggctgg ccggggccga
      301 agtccggctt cagtcccccc gaagtcatcc gctgccagag cgagtcgaga ccgccggccc
      361 tgaagtaggc gcgctctcgc cggtcggcgt cggtcgccag gtccccgtaa aggaacacgg
      421 gaacccccat cgcgccgatc gcctgtccca cctcggtggc tacttctgca gccgtctccc
      481 tccggccttc gctgagccag accaccggac atacatcgag cgccccgatc gccgggtgaa
      541 tgccatccca ggcggtcatg tcgatctcga ccgtcgacgt gccggccagc ccggtcagcg
      601 cggtgctgag agtttcggcg ggtccggcca gggtgaacac ggccctgtgg tggtcgacgt
      661 cggcgtggcg gtcgagcagt gtgacctctt cgccaagtga cgcctcgagc cggtcgagcc
      721 gcgcgaagtc ccggccctcg gagacgttcg gaaccgcgag caggctcaca ccagcaacct
      781 gccctcgagg accaccgggg tgtcgtcaat ggtgacctcc gggcgcatcg aaaccacgtc
      841 caggtggacc ggcacctgaa cgttgccgcc gatcgcggcc gacgccccga aggcgacatg
      901 ggccgtgcca atgatctctt cgtcctcgag gatgttcccg gtgagtatcg ccttctcgtt
      961 ggtgccgatt cccagctcgg cgacgttggt gccgtcgggg ccgtggacgg tgagcaactc
     1021 catcagcttc tctccttcct ctccggtcgc gtcgaccagg tgaccaccct cgatggtcag
     1081 ccggaccggc cccgaaacca gcccgacccc ggcgatcgat ccgtccacca cgagaactcc
     1141 ctcggccgtc gccggcgcga tgaatccctc tccgcagggc agatttccga aagctccggt
     1201 tgcggtcagc tcgccggcat cggggatcgc cttccgtccc tccagtccca gcgtgaggtc
     1261 gctgccgtgg tcgcaggtca gcctcgcttc ggtgccgcga tcgagcagct cggcaacccg
     1321 gtgaccacgg tggcggagct tttccatgtc gtcgctcatc acccgggcaa gcatctccac
     1381 cgtggcaccg ggaagggttg ctgtccgcgt gccggattcc gtggcacgct tccgggaggc
     1441 ggtatgtgag agcgactgaa cggtcggcgc cagcaggacg tcggctgcca ccatcgcctc
     1501 cgcaaccgtc gccggtggct cgcccgcatg cgaggcccgc tcggtcatca ccgcaagcac
     1561 cgcctcggca cccgccgact ccgcctcgct tctgagggcg tcgcccagtt ctctcgtggc
     1621 cgggttgcag accacgagca cctcctcgtt cggccgcacc ccgaggcatt cccgcacgac
     1681 ggtagccacc gcctggtcaa gttccgtgct catgccgtga atctacccag tgccgtggct
     1741 cgccccccgg cagccaaatg ctcgacggcc acaagcgcgg cggttacgct gaccccacgg
     1801 gcaattcacc aatccgagga ggtaaggatg ggactgtcga acagcaagat cgctgcggtc
     1861 gcagcggtca cggacatgga tcgggccaaa gagttcttcg aggaaacgct ggctctcgaa
     1921 ccgatgggcg gtggcgccga gggaatcgag gtcatgtacg cctgcggaga cgggaccggt
     1981 ctgctcgtct atccgtcgcc cgaccacgcc ggaaagggca cggccaccgt cgcagcctgg
     2041 gaggtcgatg atttcgatgc agagaccagc gccctgaaag aacgcggcat ctcgttcgag
     2101 atctatcccg gcttcgacca ggacgagaat ggcgtcctcg acatgggcgg agcaagggtg
     2161 gtctggttca aggatccgga cggaaacacc ttcgccgttt ccggcagctg aaaccgggtt
     2221 cccgcccggg cgggtttcgg cccgcccggg cgggtaactt ccgggcgtga agtcctggca
     2281 gcccccgctc c
//
LOCUS       DAOTIP010000335         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56096_length_2280_cov_3.344719, whole genome shotgun sequence.
ACCESSION   DAOTIP010000335 DAOTIP010000000
VERSION     DAOTIP010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56096_length_2280_cov_3.344719"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="P5138_06915"
     CDS             <1..349
                     /locus_tag="P5138_06915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRV60345.1"
                     /translation="RFGDFSRASRARTLPFPTDETETILTAVRELFDASAGEIAEKDL
                     TLIGISLGNLEGADTVQMGLPLAEDGQNARADSSALDQAVDDVRARFGEDVIKRASMI
                     DADQGVPVPTLPD"
     gene            complement(346..>2280)
                     /locus_tag="P5138_06920"
     CDS             complement(346..>2280)
                     /locus_tag="P5138_06920"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRV60346.1"
                     /translation="PGADFTVAPQQVSLDPGESASLTVSLEIDDPSELTKAVDPTAGR
                     FASTGYPRETLAEAFGRVLLEPTSPGISLRVPVYASPRPASNMSQPDSLEIHRTAATA
                     GDPEQTAGLTLSGTGVGTSSGDNGKGDGDPDNDIESIAAGFELQATGGDNPECQGSAE
                     SGCWNLPQCQTPLDSACWNLPEEKYSALKQLGYTSDAPLVSNPANARAYFAVAVQAPW
                     AIPSDKGVIQFDLDVDRDGTPDLFVYNSRLGDDDLYVSVLYDPSKPSGHRVVNTQLVN
                     GRFGDTDTALYDSDVMVLPVSLDALAGYGVDAGNPRIDYGVETYSYFSDQAIDMIGVD
                     PESGDLTDPLTANLYEPGLTVTDENGDGPLVEDQPGKELTVTRNVDSWEQDGGKGLMM
                     VHFHNRVGEKAQAVTLKGATSNTGLYLAPGTLTARVEASGSGLPEPTGQVIFSVDGSE
                     VGSAPIEDGIAVLNREVPHGATRQVLAEYPGDADFEASSDLKQRSDPTLTAKITTKGR
                     NRFGWYRKPVTIVFHCTTRGSGLTEDCPYPRRLTRDGKKLHVKKTIVAFDGGKATAKV
                     GGINIDRTAPVVRIRGVRRGATYRRIQRARCVPRDRTSGVLSCGIRWKRRGNRVIYTA
                     TATDRAGNRRVARTWIRVRR"
BASE COUNT          355 a          742 c          770 g          413 t
ORIGIN      
        1 tcgtttcggc gacttctccc gggcgagtcg tgcccgcacg cttcctttcc cgaccgacga
       61 gaccgagacc atcctcacag cggtcaggga gctgttcgat gcgtcggccg gggagatcgc
      121 cgaaaaggac ctgacgctga tcgggatctc cctcggcaac ctcgagggcg ccgacacggt
      181 gcaaatgggc ctccctctcg ccgaagacgg acagaatgcg agagctgaca gctcggccct
      241 cgatcaggcg gtcgatgacg ttcgcgccag attcggggag gacgtgatca agcgcgcctc
      301 gatgatcgac gcggaccagg gagtcccggt cccgacgctt cccgactagc gcctgacccg
      361 gatccaggtc cgcgccaccc gccggttgcc ggcccggtcg gtggcggtgg cggtatagat
      421 gacccggtta ccgcgccgtt tccagcggat tccgcaactg aggacgccgg aggtgcggtc
      481 gcgcgggacg caacgggccc gctgtatccg gcggtaggtg gcaccccgcc ggactccgcg
      541 gatccggacc accggggcag tccggtcgat gttgattccg ccgaccttcg cggtggcctt
      601 gccgccgtcg aaggcgacga tggtcttctt cacatgcagc tttttcccgt cccgggtcag
      661 gcgcctgggg tacgggcagt cttcggtcag gccggaaccg cgggtggtgc agtggaagac
      721 gatggtgacc ggtttccggt accagccgaa cctgttccgg cccttggtgg tgatcttcgc
      781 ggtcagggtc gggtcggagc gctgcttcag gtccgaagaa gcctcgaaat cggcgtcacc
      841 cggatactcg gcgagcacct gtcgcgtggc gccatgaggc acttcgcggt tcaggacggc
      901 tatcccgtcc tcgatcgggg cggaacccac ctcgcttccg tcgaccgaga agatcacctg
      961 gcccgtcggc tccggcaatc cgctgcccga tgcctcgacc cgggcggtaa gcgtgccggg
     1021 cgccagatag agaccggtat tggaggtggc ccccttcagc gtgacggcct gggccttctc
     1081 gccgacccgg ttgtggaagt ggaccatcat cagtcccttg ccgccgtcct gctcccagga
     1141 atcgacgttc ctggtcactg tgagctcctt gccgggctgg tcctcgacca gtggtccgtc
     1201 accgttttcg tcggtgaccg tcaggcccgg ctcgtagagg tttgcggtga gaggatcggt
     1261 caggtctccg gattcggggt ccactccgat catgtcgatc gcctggtcgg agaagtagga
     1321 ataggtctca accccgtaat cgatccgcgg gttcccggcg tcgacgccgt agccggccag
     1381 agcatcgagt gagaccggca ggaccatcac gtcgctgtca taaagggccg tgtctgtgtc
     1441 accgaagcgc ccgttgacca gctgggtgtt gaccaccctg tgcccggacg gcttcgacgg
     1501 gtcgtacagc accgacacat agaggtcgtc gtcaccgagc cgcgagttgt agacgaagag
     1561 gtcgggggtg ccgtcgcgat cgacgtcgag atcgaactgg atcactccct tgtccgaggg
     1621 aatcgcccac ggagcctgga ccgccaccgc gaagtaggca cgggcgttcg ccgggttgct
     1681 gaccagtggt gcgtcagagg tgtatccgag ctgcttcaga gcggaatact tctcctcggg
     1741 cagattccag caggcactgt cgagcggcgt ctggcattgc ggcagattcc aacagccact
     1801 ctcggccgac ccctggcatt ccggattgtc gccaccggtc gcctgaagtt cgaagccggc
     1861 cgctatcgac tcgatgtcgt tgtccggatc gccgtcgccc ttgccgttgt caccgctcga
     1921 agttccgact ccggttccgg agagcgtcag acccgccgtc tgctccgggt ccccggcggt
     1981 agcagccgtg cgatggatct ccagagagtc gggctggctc atgtttgatg ccggcctcgg
     2041 cgaggcgtac accggcaccc gcagggaaat gccggggctg gtcggctcga gcagaacccg
     2101 accgaaggcc tcggccaggg tctcacgcgg gtagccggtc gaggcgaacc ggcccgccgt
     2161 cgggtcgact gccttggtca gctcggaggg atcgtcgatt tcgaggctca cggtcagact
     2221 cgctgactcg cccggatcga ggctgacctg ctgcggcgcg accgtgaagt cggcgccggg
//
LOCUS       DAOTIP010000336         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56197_length_2277_cov_3.285779, whole genome shotgun sequence.
ACCESSION   DAOTIP010000336 DAOTIP010000000
VERSION     DAOTIP010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56197_length_2277_cov_3.285779"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="P5138_06925"
     CDS             complement(<1..394)
                     /locus_tag="P5138_06925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRV60347.1"
                     /translation="MDSASGPRASVRRRIEFSDVDSSGRYHYGTVIRLIEAAEVELLA
                     ELGLLDSIYTSMPRARLEVDYVSTLRFLDEVEITVEVVDLGRTSVTFGFVIERDGDVC
                     ARAKLITVHVDGDGNPAPWPEQARACLAG"
     gene            complement(555..1274)
                     /locus_tag="P5138_06930"
     CDS             complement(555..1274)
                     /locus_tag="P5138_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV60348.1"
                     /translation="MRAVTYDFWNTLMWAEPGMMGELRRGQIELAAEGIGLELPPDAI
                     EQGLLAIGELHSEAWMRGELFNPRDGADHFIASLGEGTAEKHADALTAALLLDSVQPD
                     LNIAPEVEDTLEVLKENGIRTAIVCDVGLAPSTVLRRSLESAGLIGLFDAWAFSDEVG
                     HYKPSPEIFRHALESIEVAPEHAVHVGDLRRTDVAGSRSFGMTAVRYTGLLEDDDHES
                     AEADHVITSHSEILKILGLSA"
     gene            1298..1669
                     /locus_tag="P5138_06935"
     CDS             1298..1669
                     /locus_tag="P5138_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRV60349.1"
                     /translation="MPSDSELVWSSDGGDFRPEQKQGKKKRGRSGKSAAAPAPGPAPA
                     GSTIKVKRETSGRKGKTATTISEIPLGEAETKALAKKLKQHCGVGGTARGSSIELQGD
                     HRDKVITFLEKEGHRAVKAGG"
     gene            complement(2000..>2277)
                     /locus_tag="P5138_06940"
     CDS             complement(2000..>2277)
                     /locus_tag="P5138_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRV60350.1"
                     /translation="VNDIAAGILMVRFYNDPDVLNEPAVALQRAHLWMRDLSDSQLEH
                     FLDAHPTLHSKIKEALDRAAVKGHPFSVNAEWLSHPHKWAGFATFGS"
BASE COUNT          477 a          694 c          684 g          422 t
ORIGIN      
        1 agccggcaag gcaggccctg gcctgctcgg gccatggagc ggggttcccg tcaccgtcga
       61 cgtggacggt gatcagcttc gcccgcgcgc agacgtcgcc gtcccgttcg atcacaaaac
      121 cgaaggtgac cgaggtcctg ccgaggtcga ccacctccac ggtgatttcg acctcatcaa
      181 ggaaacgcag ggtcgagacg tagtcgacct cgagccgcgc ccgcggcatc gaggtgtaga
      241 tcgaatccag caacccgagt tccgcaagca gttccacctc ggccgcctcg atcaacctga
      301 tcacggtccc gtagtggtac cggccggaag agtcgacatc agaaaactcg atccgtcgtc
      361 gaacggaagc gcggggtccg gaagcggaat ccatcgggat cagaatctat tgaatcgggg
      421 cagggcagtc tggtccggcg gccatgtttc ttcacgattc gaaaccctgg ccgggttgcg
      481 cagtgagctc ccggtctgca ggccggcgac cccattctca aggtcaccgt cccgcagaca
      541 ccccgacccc aatttcaggc ggaaaggccc agaattttca ggatttcgct gtggctggtg
      601 atcacatggt cggcttcggc tgactcgtgg tcatcgtcct cgagcagccc ggtatagcga
      661 accgcggtca ttccgaagct tcgggaacca gcgacatcgg ttcgccggag atcaccgaca
      721 tgaacggcgt gctcgggggc gacttcgatc gattccaggg cgtgcctgaa gatttccggt
      781 gacggcttgt agtgaccgac ctcgtccgag aaggcccagg catcgaatag tccgatcagg
      841 cccgcgcttt cgaggcttcg gcgaaggacc gttgaagggg caagcccgac gtcacatacg
      901 atcgcggtcc ggattccgtt ctccttcagg acctcaagag tgtcctcaac ttcgggagcg
      961 atgttcaggt caggctgtac cgagtccagc agcaaggctg cggtcagtgc gtccgcatgt
     1021 ttctccgcgg tcccctctcc aagcgacgcg atgaaatggt cggctccgtc acgggggttg
     1081 aaaagctcac ctcgcatcca ggcttcgctg tgcaactcac cgatcgcaag cagtccctgt
     1141 tcgatcgcat ccggaggcag ttccagcccg atgccttccg ccgccagttc aatctggccg
     1201 cgtcgaagct cccccatcat cccgggctcc gcccacatca gggtgttcca gaagtcgtag
     1261 gtgactgctc tcaagggacc cagaataggg ttaccgcatg ccttccgact cggaactcgt
     1321 ctggtcaagc gacggcgggg attttcgccc cgaacagaag cagggcaaga agaagcgggg
     1381 tcgcagtgga aagtctgccg ccgcaccggc ccccggcccg gctccggccg gttcgacgat
     1441 caaggtgaag cgggagacct cgggtcgcaa gggcaagacc gccaccacga tatccgagat
     1501 cccgctcggc gaagctgaaa cgaaggccct ggcgaaaaag ctgaagcagc attgcggggt
     1561 cggcggaact gcacgtgggt cgagcatcga gcttcaggga gaccatcgcg acaaagtgat
     1621 caccttcctc gaaaaggaag ggcaccgggc cgtgaaggcc gggggctgag ggaataccgg
     1681 aagacgatcg cttccttccc ctcaaccaaa ctggccctca aaatgagggc ggcaaacagc
     1741 agatgagcca atcggggcga ttggtccaat ttcgtgctca tctggctcag ctcctgactt
     1801 tgaacaattc cgttcgcact acctttctca ctacccgcat ggtccacgcg gatccgaggt
     1861 tcgttggcag gcgtgtttcc agcaatttta ccgcgccgga acaccgaaca agaaactccc
     1921 agatagttca tccaacccgt aacgcgccgg ccgaggggac caaactgcgg gtgctgttca
     1981 ggacgtattc gacatttcgc taggaaccga atgttgcgaa tcctgcccac ttgtgcgggt
     2041 gagatagcca ttcggcgttg accgagaaag ggtggccctt cacggcagca cgatctagag
     2101 cttccttgat cttggaatgg agagtcggat gcgcgtccag gaagtgttct aattgactat
     2161 cggacaagtc tcgcatccag agatgtgcgc gttggagagc gaccgccggc tcattgagga
     2221 catccgggtc gttataaaaa cgaaccatca ggattcccgc tgcgatgtcg ttgactc
//
LOCUS       DAOTIP010000337         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56529_length_2267_cov_3.469259, whole genome shotgun sequence.
ACCESSION   DAOTIP010000337 DAOTIP010000000
VERSION     DAOTIP010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56529_length_2267_cov_3.469259"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..590)
                     /locus_tag="P5138_06945"
     CDS             complement(30..590)
                     /locus_tag="P5138_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRV60351.1"
                     /translation="MARLPYPEPETLPQQCQEILDALPDLNIFHMLAHAETALAPSLE
                     LGGTILSRLELDPVSRELVILLVAKLSEADYEWVQHVGIAEAIGIPGESVAAIERADF
                     DSPALDERSRALLRFTEQATTDPRVSNETFANLRALFSPREIVETLMVIGYYLMLARV
                     MTVLDIELDEAIGSRVIREAEHRQQP"
     gene            complement(590..1405)
                     /locus_tag="P5138_06950"
     CDS             complement(590..1405)
                     /locus_tag="P5138_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV60352.1"
                     /translation="MTKPALEKDGRVPGRGRLENRRVLILGAGQQEYNLEDPPAGNGR
                     AISQLIAREGASTALADLDEEALHATAEMVEGEGVAPIELVGDFANEEDLGRVVSEAA
                     DDMNGLDGLVANVGIVGGWGLEQTGAEEWDRVFAVNVRAHYLACRNALPLMDDGSSIV
                     LISSIAAEMPANPVVAYHSSKSALVGLARWLAKEVAPRGIRVNIVVPGLIDTSLGRLA
                     SQAVEHREKMPVPLGRQGTAWEVAYATVFLLSDEASYITAQSLLVDGGLVDLR"
     gene            1478..2083
                     /locus_tag="P5138_06955"
     CDS             1478..2083
                     /locus_tag="P5138_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV60353.1"
                     /translation="MSRECKRVRPTRAETRDRLLDSAARVFSKKGFAQASLDDVAADA
                     GLTKGAVYSSFESKDALFFALMKKRIDQRLELIESLGRESQDSMPEAIDRFAEMGRAQ
                     DDWHLLFIEFWSRAVRDPDLHADFVRHRRAARASIARLLQVQSEKLGISLPASPDDLA
                     VGLLALSNGMAIERIGDPEEIDEHQFSLAVRLLLRPATGRQ"
BASE COUNT          378 a          708 c          727 g          454 t
ORIGIN      
        1 ggcggccctc acgggccgcc ctttcgcgtt tagggctgct gcctgtgttc ggcttcccgg
       61 atgacccggc tgccgatcgc ttcgtcgagt tcgatgtcga gaacggtcat gacccgggcc
      121 agcatgaggt agtagccgat gaccatcagc gtctccacga tttcccgtgg agaaaagagc
      181 gctctcaggt tcgcgaaggt ctcgttgctg acccgagggt cggtcgtcgc ctgttccgtg
      241 aacctgagca gcgctctgga tcgttcgtcg agcgcggggg aatcaaagtc ggctcgctct
      301 atcgctgcca ccgactcgcc cgggatgccg atcgcttcgg ctatcccgac gtgttgaacc
      361 cattcgtaat cggcttccga gagcttcgcg acgagcagga tcacgagttc gcgcgagacc
      421 gggtccagtt ccagccggga aaggatcgtg cccccgagct cgagcgacgg agcgagcgcc
      481 gtctcggcgt gagccagcat gtgaaagatg ttgagatcgg gcagggcgtc gagaatctcc
      541 tggcattgtt gagggagagt ctctggctcg gggtagggaa gtcgggccat cagcgcaggt
      601 caaccagtcc gccgtcgacc aggagcgatt gagcggttat gtagctggct tcgtccgaga
      661 ggaggaagac cgtggcatag gcgacttccc aggcggtgcc ctgtcgaccg agcggtacgg
      721 gcatcttctc ccggtgctcc accgcctggc tggccagcct gcccagggag gtatcgatca
      781 acccggggac cacgatgttg acccggattc cgcggggggc aacttccttg gcgagccatc
      841 gggccagtcc gaccagcgcc gacttcgaag agtggtaggc gacgaccggg ttggctggca
      901 tctccgcggc gatcgacgag atcagaacga tcgatgaacc gtcgtccatg aggggaagcg
      961 cattcctgca ggccaggtag tgagcccgga cgttcaccgc gaagacccgg tcccattctt
     1021 ccgctccggt ctgttccagg ccccagccac cgacgatccc cacgttggcg accaggccgt
     1081 ccagcccgtt catgtcgtct gccgcttcgg aaacgacccg gccgagatcc tcctcgtttg
     1141 cgaagtcgcc gaccaactca atcggggcca cgccctcgcc ttcgaccatt tcagcggttg
     1201 catgcagcgc ttcctcgtcc aggtcggcca gtgccgttga tgcgccttcc cgcgcaatca
     1261 gctggctgat ggccctgccg ttgccggctg gcgggtcctc caggttgtac tcctgctgcc
     1321 cggcgcccag gatcaggacc cgacgatttt ccagtcgccc tcttcccggg actcttccgt
     1381 ctttctccag ggccggcttt gtcattgctc gcacgtttcc tcgccgtaga tttccatacc
     1441 gatcagtatt gaatactagc gggtattgta tgtctccatg tctcgtgagt gcaagagagt
     1501 ccggccgacc cgggccgaga caagagaccg cctccttgac tccgcggcca gggtgttctc
     1561 gaaaaagggt ttcgcccagg cgtcgctgga cgatgtggct gcggacgccg gtctgaccaa
     1621 gggagccgtc tattcgagct tcgagagcaa ggacgcgctc ttctttgccc tgatgaagaa
     1681 gcggatcgac cagagactcg aactgatcga atcacttggc cgggagagcc aggattcgat
     1741 gcccgaggcg atcgacaggt tcgccgagat ggggcgagcg caggacgact ggcatctgct
     1801 cttcattgaa ttctggtccc gggccgtccg ggatcccgat ctgcacgccg attttgtccg
     1861 ccatcgtcgg gcagcccggg catcgatcgc caggctgctg caggttcagt cggagaagct
     1921 cgggatttca ttgcccgctt cgccggatga tctggccgtg gggctcctgg cgctttccaa
     1981 cggaatggcc atcgaacgga tcggcgaccc cgaggagatc gacgagcatc agttcagcct
     2041 tgcggttcga ctgttgctgc gaccggcgac tggccggcaa tgaggtatcg acccgagtcg
     2101 gagagtggtt tgtcctcctc cgcccgggag tccggccgcc agcgaagggt cgtcagcggc
     2161 gggctacttt gatcgtgtag gccgcatcgt tgccagtttg tcttctggac ggggtcaggg
     2221 cgagccagac cgactgggga cgcttcttca ggttccgcac cacgatc
//
LOCUS       DAOTIP010000338         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56593_length_2265_cov_3.494570, whole genome shotgun sequence.
ACCESSION   DAOTIP010000338 DAOTIP010000000
VERSION     DAOTIP010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56593_length_2265_cov_3.494570"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="P5138_06960"
     CDS             <1..754
                     /locus_tag="P5138_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV60354.1"
                     /translation="HFHSDHIGGLGSVNLQRWVAEGTDTPMRVLGPPGVERVVKGYND
                     AYRLDRSYRTAHHGEEFVDPAIGTMVPEEFPVPDEGEANVVLEDDGLVVTAFEVDHSP
                     VTPVVGFRFDYKGRSAVISADTVYSGNLVKASRNTDLLLHDALSQELLLMVAKAQTDA
                     GNPARGKILADVTDYHATAPQAADAARQAGARALALTHVVPPLPLKGLEEVYLADAPK
                     RFDGPLWIAADGDLYSLPPEGGLKRSNLIKRR"
     gene            764..1162
                     /locus_tag="P5138_06965"
     CDS             764..1162
                     /locus_tag="P5138_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRV60355.1"
                     /translation="MGLDRNKELVRRFYAEAINQRDRTACERLLTEDFTHDGEVKGRA
                     GQQEVVNAFLDAFSDLRNEILMLVAEGDLVAAHQRWTGTQDGEFMGMPTTGRKVEFTS
                     TAIIRIEDGLVAEAWDEVDLFGLSAQLKPE"
     gene            1261..1629
                     /locus_tag="P5138_06970"
     CDS             1261..1629
                     /locus_tag="P5138_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60356.1"
                     /translation="MLALSSLAIALPAAFAPETFYTDFPFYTALVKLLPPYNEHLITD
                     IGGLYLGFGLMFIWAMIKPSKELVVPLCWGWIAAQALHFAFHVSHLTGFTTTEAVGQQ
                     IGLALYIAVALVPIAMLRRG"
     gene            1639..>2265
                     /locus_tag="P5138_06975"
     CDS             1639..>2265
                     /locus_tag="P5138_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRV60357.1"
                     /translation="MELCVMIEGQEDVSWEDWTAIARSCEENGIPALFRSDHYLSVFP
                     SEGRQSLDAWATISGLAAITSSLRLGTMVSPASFRHPSILAKMIATADRISGGRIEPG
                     IGAGWHQAEHDAFGFPFLPVRERMKVLEEQLEIVTGLWSSGEFEFHGDYYDLAPVDAR
                     PKPVQDPMPLVMGGNAAPKGAALAARFASEYNTPNPTLEQARERRAAIE"
BASE COUNT          401 a          783 c          712 g          369 t
ORIGIN      
        1 ccattttcac tccgaccaca tcggtgggct gggctcggtc aacctgcagc gctgggtcgc
       61 cgagggaacc gacacgccga tgcgggtact gggaccgccc ggtgtcgagc gggtggtcaa
      121 gggctacaac gacgcctacc ggctcgaccg cagctaccgc accgcacatc acggggaaga
      181 gttcgttgac ccggcgatcg gtaccatggt tccggaagag tttccggtcc cggatgaagg
      241 cgaggcgaac gtcgtgctcg aggacgacgg cctggtcgtc accgctttcg aggtggacca
      301 ttccccggtc accccggtgg tcggcttccg cttcgactac aagggacgga gcgcggtgat
      361 cagcgccgac acggtctatt cgggaaacct ggtcaaggca tccaggaaca ccgatctgct
      421 gcttcacgac gcgctatcgc aggagctcct gctgatggtg gcgaaggccc agaccgacgc
      481 cggcaacccg gcccggggca agatcctcgc cgacgtgacc gactaccacg cgaccgcccc
      541 ccaggccgct gacgccgccc gccaggccgg ggccagggcc ctcgcgctca cccatgttgt
      601 tcccccgctt ccgctcaagg gcctcgaaga ggtctatctc gccgacgccc cgaagcggtt
      661 cgacggaccg ctctggattg ccgccgacgg cgacctctac agcctgcccc ccgaaggcgg
      721 cctgaagcgc agcaacctga tcaagcgccg atagtctcgg cccatgggcc tcgaccggaa
      781 caaggaactg gtgcgccgct tctacgcaga ggcgatcaac cagcgggacc ggaccgcctg
      841 cgaaaggctg ctgaccgagg acttcaccca cgacggcgaa gtcaagggaa gggccggcca
      901 gcaggaagtt gtgaacgctt tcctcgacgc cttttcggac ctccgcaacg agatcctcat
      961 gctggtcgcc gaaggtgacc tcgtcgcggc ccaccagagg tggaccggga cccaggacgg
     1021 cgaattcatg ggcatgccga cgaccggacg caaggtcgaa ttcacctcga ccgcgatcat
     1081 ccggatagag gacgggctgg tggccgaggc ctgggacgag gtcgacctgt tcggtctctc
     1141 ggcccagctg aagccggaat gaggttcacc gtcgctggtc cccgctaggt ttcggcaatc
     1201 gcaacccggt caaagaagaa acaacaggca gacgcgccgg cgatcaggat ctgtcttgga
     1261 gtgctggcgc tttcgtccct cgcgatcgcg ttgccggccg cgtttgcccc ggaaaccttc
     1321 tacaccgact tccctttcta cacggcgctg gtcaagctgc tgccgcccta taacgagcac
     1381 ctgatcaccg acatcggcgg gctctacctc ggtttcggcc tgatgttcat ctgggcgatg
     1441 atcaagccct cgaaggagct ggtggttccg ctctgctggg gctggatcgc cgcccaggcc
     1501 cttcacttcg ccttccacgt gagtcacctg accggcttca cgacgaccga agcggtcggc
     1561 cagcagatcg gcctcgccct gtacatcgcg gtcgcgctgg taccgatcgc gatgctgcgc
     1621 cgcgggtagg ctcgggccat ggaactctgc gtgatgatcg aaggccagga agacgtttcc
     1681 tgggaggact ggacggcaat cgccaggagc tgcgaggaga acggcatacc ggcccttttc
     1741 cgttccgacc actacctgtc ggtcttcccc tcggaaggca ggcagtcgct cgacgcctgg
     1801 gccacgatca gcggactggc tgcgatcacc tcaagcctgc gcctcggaac catggtctcc
     1861 ccggcctcct tccgccaccc ctcgatcctg gcgaagatga tcgccaccgc cgaccggatc
     1921 tccggcggcc ggatcgaacc gggcatcgga gccggctggc atcaggcgga acacgatgct
     1981 ttcggcttcc ccttccttcc cgttcgcgag cgaatgaagg tcctcgaaga gcagctggag
     2041 atcgtcaccg gcctgtggtc gtcgggtgag ttcgagttcc acggcgacta ctacgacctc
     2101 gccccggtcg acgccaggcc gaagccggtc caggacccca tgccgctcgt catgggcggc
     2161 aacgctgcgc cgaaaggcgc cgctctcgcc gcccgtttcg ccagcgaata caacacgccc
     2221 aacccgaccc tcgagcaggc gcgcgagcgg cgggcggcga tcgag
//
LOCUS       DAOTIP010000339         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56599_length_2265_cov_3.186425, whole genome shotgun sequence.
ACCESSION   DAOTIP010000339 DAOTIP010000000
VERSION     DAOTIP010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56599_length_2265_cov_3.186425"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="P5138_06980"
     CDS             complement(<1..702)
                     /locus_tag="P5138_06980"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRV60358.1"
                     /translation="MDTADRIHVTRSRANPGGGDVQLDDAIPFRSRKPSWLKVPPPGG
                     PVYREIKQMIDGESLNTVCEEANCPNIGECWERGTATFMILGDVCTRRCGFCNVQTGK
                     PTHNDPLEPARVAMQVKRLGLSHAVITSVDRDDLPDYGASAFVGVIRSIRAQAPGCKV
                     EILTPDFRGQEMPLARVVHERPDVFNHNVETVPRLYPKARRGSKFLRSARVLRLAREM
                     GEGEVRRLSTFEYRNI"
     gene            complement(710..1513)
                     /gene="lipB"
                     /locus_tag="P5138_06985"
     CDS             complement(710..1513)
                     /gene="lipB"
                     /locus_tag="P5138_06985"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRV60359.1"
                     /translation="MAALTVIDLGRREYAEMVALQERLAELRLEGSVGDLLLLLEHPP
                     TYTLGRRSEPSDLLHDSGWYEERGIAICETPRGGKVTYHGPGQLVAYPIVDLRGVGAQ
                     PSGADRVDVAGFVAALEQSMSGALAEWGLATGRIEGLTGLWVDDDRPDRADFEGADAA
                     SAAPGLARGSIRKIGSIGLKIRRGVSTHGLSINVSCNLDPFDWINSCGIEACRATSIA
                     AELGEEGPSVAAVGAAVAGRLADGLGLKTTEGRAAEIDLTEAQRVDSVA"
     gene            complement(1495..1929)
                     /locus_tag="P5138_06990"
     CDS             complement(1495..1929)
                     /locus_tag="P5138_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60360.1"
                     /translation="MSDGPATADPGAGPKCVKSSAGVSGGRRVFRSAGVAGSVGVTHS
                     AGVAGSAGAGFSAGVAGSAIVLFSSAVTFSFAVLGSTLMAFCAFCVGCARCRRCAFCV
                     GCRDCVDCVGCVGCSGLVGARGVVGKSARKNRSGLCRWLPSR"
     gene            complement(1926..>2265)
                     /locus_tag="P5138_06995"
     CDS             complement(1926..>2265)
                     /locus_tag="P5138_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRV60361.1"
                     /translation="DLNEIAAEVRSLSTRARSGEITPPEQAGATFAISNLGMLGVTSS
                     EPVVNPGQAAKLAVGVVTERPVVRGGEVVPGDVMTLDLSCDHRVLHGAEAARFLNEVK
                     ANLENPGLLG"
BASE COUNT          361 a          780 c          795 g          329 t
ORIGIN      
        1 gatgttgcgg tactcaaatg tggaaagtcg ccgcacctcg ccttcgccca tctcgcgggc
       61 caggcggagc acccgggccg agcgcagaaa cttcgagcca cgcctggcct tcgggtagag
      121 ccgcggcacg gtctcgacgt tgtggttgaa cacgtcgggc cgctcgtgga cgacccgggc
      181 aagcggcatc tcctggccgc ggaagtcggg agtcaggatt tcgaccttgc agcccggggc
      241 ctgggcgcgg atcgagcgga tcaccccgac gaaggcggag gctccgtaat cgggcaggtc
      301 gtcgcggtcg accgaggtga tcaccgcgtg ggagaggccg agccgcttga cctgcatcgc
      361 gacccgggcc ggctccagcg ggtcgttgtg ggtcggcttg ccggtctgga cgttgcagaa
      421 gccgcagcgc cgggtgcaga cgtcgccgag gatcatgaag gtggcggtcc cgcgctccca
      481 gcactcaccg atgttcgggc agtttgcctc ctcgcagacg gtgttcaggc tctcgccgtc
      541 gatcatctgc ttgatctcgc ggtagaccgg gccgccgggc ggggggacct tcagccagga
      601 aggcttgcgg gaacggaacg ggatagcgtc gtcaagctgg acgtcaccgc cgccgggatt
      661 ggcgcgggaa cgggtgacgt gaatgcggtc tgcggtgtcc accggtctcc taggctacgg
      721 aatcgacccg ctgcgcttcg gtgagatcga tctccgcggc cctgccctcg gtcgttttga
      781 gtcccaaccc gtcggccagc cgcccggcga cggcagcgcc gaccgcggcc accgacgggc
      841 cttcctcgcc gagctcggcc gcgatcgagg tggcgcgaca ggcctcgatg ccgcaggagt
      901 tgatccagtc gaaggggtca aggttgcagg aaacattgat cgagagcccg tgggtgctga
      961 ccccgcggcg gatcttcagg ccgatcgaac cgatcttgcg gatgcttccc cgggcgaggc
     1021 ccggtgcggc cgacgcggca tcggccccct cgaagtcggc gcggtccggg cggtcgtcgt
     1081 ctacccagag accggtcagg ccctcgatcc ggccggtcgc gaggccccac tcggcgagtg
     1141 cccccgacat cgactgctcc agggcggcga cgaagccggc gacgtcgacc cggtcggcgc
     1201 cggacggctg ggcgccgact ccccgcagat cgacgatcgg gtaggcgacc agttggcccg
     1261 gcccgtggta ggtcactttg ccgccacggg gggtctcaca gatcgcgatc ccgcgctcct
     1321 cgtaccagcc ggaatcgtgg aggaggtcgg agggctcgga gcggcggccc agcgtgtagg
     1381 tgggcgggtg ctcgagcaac agcagcaggt ccccgaccga accttccagg cgaagctcgg
     1441 ccagtcgctc ctggagagcc accatctccg cgtactcgcg gcggccgaga tcgatcaccg
     1501 tgagggcagc catcggcaca gtcccgaacg gttcttcctg gctgacttgc cgaccacccc
     1561 tcgtgcaccg accagcccgg aacagccgac acaaccgaca cagtcgacgc agtcacggca
     1621 gccgacgcag aaggcgcagc gccggcaccg ggcgcatccg acgcagaagg cgcagaaggc
     1681 catcagggtg gagccgagca ccgcgaagct gaaggtgacc gccgagctga aaaggacgat
     1741 ggcgctgccg gcgaccccgg cactgaaccc ggccccggca ctgccggcaa ctccggcgct
     1801 atgggttacg cccacgctgc cggcgacgcc ggccgaccga aagacccggc gacccccgga
     1861 aaccccggcg ctacttttca cgcacttcgg gcccgcgccg ggatcggctg tcgcgggccc
     1921 gtcgctcatc cgagcagtcc cggattctcg aggttcgcct tgacctcgtt caggaaacgg
     1981 gcggcttcgg cgccgtgaag gacacggtgg tcgcaggaaa gatccagggt catcacatcg
     2041 cccgggacca cttcgccacc ccggacgacc ggccgttcgg tgaccacgcc gaccgccagc
     2101 ttcgccgcct gtccgggatt cacgaccggc tcggaactgg tgactccgag catgcccagg
     2161 ttggagatcg cgaaggtcgc gccggcttgc tcgggagggg ttatctcgcc ggagcgtgcc
     2221 cgcgtggaga ggctgcgcac ctcggccgcg atctcgttca ggtcc
//
LOCUS       DAOTIP010000340         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56950_length_2255_cov_3.574091, whole genome shotgun sequence.
ACCESSION   DAOTIP010000340 DAOTIP010000000
VERSION     DAOTIP010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56950_length_2255_cov_3.574091"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="P5138_07000"
     CDS             complement(<1..538)
                     /locus_tag="P5138_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRV60362.1"
                     /translation="MSNGVHIFWILSRGAGIAAILFAGLSVTIGLLSSRGMPFPKLRK
                     NFELRPLHEALSMATIVLVAAHGLILLGDPWLKPGLAGISIPFVMSYRSLWTGIGIIA
                     GYGLALLGLSYYLRRWIGPSRWRTAHRFIAIFWLLGLVHTFGAGTDAFQPWVWIPVAL
                     TSGPALVLLIMRLTQRSSK"
     gene            complement(531..1448)
                     /locus_tag="P5138_07005"
     CDS             complement(531..1448)
                     /locus_tag="P5138_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRV60363.1"
                     /translation="MNAQVTTLDFPALGGKVLIQAEGPGSDLAVEEAREMVLDLHRRL
                     TRFEESSELCRLNANSEQSVAASPLLLRFAEDVRLAGEMSGGLVDATLLDQVEAAGYT
                     ESIDPDSPLEPRPETPTATVPARQWNWSDVKVDRDASAVIRPPGLRLDSGGIGKGLAA
                     DIVAERLRDFDSWAVVCAGDLRFGGTNGAERRIDVTSPFPDAGVIADYTAPSGAIATS
                     GTTRRSWEVESGRSHHLIDPRTGLPARTGLVQVTAVAPTAVEAEVRAKAALLSGPDRA
                     SEWLIDGGMFVTDSGEIRAWDFRRRPGRE"
     gene            complement(1445..1837)
                     /locus_tag="P5138_07010"
     CDS             complement(1445..1837)
                     /locus_tag="P5138_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60364.1"
                     /translation="METLNGKELAEARKRARGIRVRNIRRRIAYGATVMVAVFSGAIL
                     FRSLEQQTGSTATVTSSVTRSDDGESSDGLSSLANQVASAFGDDGEEEGSGSTSTTVA
                     PSTTTVTPPTTTVTPPTTTTAPLTTSQS"
     gene            complement(1907..>2255)
                     /locus_tag="P5138_07015"
     CDS             complement(1907..>2255)
                     /locus_tag="P5138_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60365.1"
                     /translation="NNPIGRWVAVGAAVAVAFFSGLLIGRATQENSSEADLASFGRPE
                     GSGPPMGGQVPFQDGQGFGDQQYGHEDGDSFGPPQSGDDYGNGEEGSMPQGGDQSDSS
                     GGSTPAPLESGQS"
BASE COUNT          373 a          713 c          790 g          379 t
ORIGIN      
        1 gcttgctgct ccgttgggtc aagcgcatga tcagcagcac cagggccggg cccgaagtga
       61 gcgcaaccgg gatccagacc cagggctgga aggcgtcggt gccggcgccg aaggtgtgga
      121 ccaggccgag cagccagaag atcgcgatga aacggtgagc ggtgcgccag cgcgacggcc
      181 cgatccagcg gcgcaggtag tagctcagac cgagcagggc cagtccgtag ccggcgatga
      241 tcccgatccc ggtccaaagc gagcggtagg acatcacgaa gggaatggag atcccggcca
      301 ggccgggctt cagccacggg tcgccgagca ggatcaggcc atgagcggcg accagcacga
      361 tcgtcgccat tgaaagcgcc tcgtggagag gcctgagttc gaagttcttc ctcagcttcg
      421 gaaaaggcat gccccggctg ctgagcaggc cgatggtcac cgagagcccg gcaaagagga
      481 tcgccgcgat acccgcaccg cggctgagga tccagaagat gtgaacgcca ttactcacgg
      541 cccggcctcc gtcggaaatc ccaggcgcgg atctcgccgc tgtcggtcac gaacatgccg
      601 ccgtcgatca gccattcgga ggcgcggtcg ggtccggaaa gcagggctgc cttcgcccgc
      661 acctcggctt cgaccgcggt gggggccacg gcggtcacct ggaccagtcc ggtccgggcc
      721 ggcaggccgg tgcgggggtc gatcagatgg tgggagcgtc ccgattcgac ctcccaggag
      781 cggcgggtcg tgccactggt ggcgatcgcg cccgacgggg cggtgtagtc ggcgatcacc
      841 ccggcatcgg ggaaagggga ggtgacgtcg atccggcgct cggcaccgtt ggttccgccg
      901 aaccgaaggt cgccggcaca tacaactgcc caggaatcga agtcccgcag cctctcggcg
      961 acgatgtcgg cggccaggcc cttgccgatg cccccggaat ccaggcgcag cccgggaggg
     1021 cggatcacgg ccgatgcgtc gcggtcgacc ttcacgtcgg accagttcca ttgcctggcc
     1081 gggacggtcg ccgtgggagt ttccggtcgc ggctcgagcg ggctgtccgg atcgatcgat
     1141 tcggtgtatc cggcggcttc gacctgatcg agcagcgtgg cgtcaaccag accgccgctc
     1201 atctcgccgg ccaggcggac atcctcggcg aagcgcagga ggagcgggct cgcggccacg
     1261 gactgttcgg agttggcgtt cagccgacag agctcgctcg actcttcgaa gcgggtcagc
     1321 cgccggtgca ggtcaagaac catctcccgg gcctcttcga ccgcgaggtc ggatccgggc
     1381 ccttcagcct ggatcaggac cttgccgccg agggccggga agtcgagggt cgtgacctgt
     1441 gcgttcatga ctggctggtg gtcagcggag cagtggtggt cgtcggcgga gtgacggttg
     1501 tcgtgggagg agtgaccgtc gtggtggagg gggccacggt ggttgaagtc gagccggaac
     1561 cttcctcctc gccgtcgtcg ccaaaggccg atgcaacctg gttcgcgagt gagctcagtc
     1621 cgtctgagct ctcaccgtca tcactcctgg tcaccgatga agtgaccgtc gcggtcgagc
     1681 ctgtctgctg ttcgagagaa cggaacagga tggcgccgct gaagacggcc accatcactg
     1741 ttgctccgta tgcgatccgg cgccggatgt tcctgacgcg aatccctcgg gcccgctttc
     1801 ttgcctctgc cagttctttt ccgttcagtg tttccatggt tggtaaaggt tcgaatatca
     1861 ggcttggggg agtgtgaatc gcgcgtgaga attctctgaa acttgctcag gactggccgc
     1921 tctccagcgg ggcgggggtt gaacctccgg aagagtcact ctggtctccg ccctgcggca
     1981 tggagccctc ctcgccgttg ccgtagtcat cacctgactg cggcgggccg aagctgtctc
     2041 cgtcttcgtg tccatactgc tggtcaccga agccctggcc gtcctggaag ggaacctggc
     2101 cgcccatcgg gggaccgctg ccttccggcc ggccgaacga ggccaggtcg gcttccgaac
     2161 tgttctcctg ggtggcacgc ccgatcagga ggccgctgaa gaaggcaacc gctaccgcgg
     2221 cgccgaccgc gacccagcga ccgatggggt tgttg
//
LOCUS       DAOTIP010000341         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56957_length_2255_cov_2.875909, whole genome shotgun sequence.
ACCESSION   DAOTIP010000341 DAOTIP010000000
VERSION     DAOTIP010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56957_length_2255_cov_2.875909"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="P5138_07020"
     CDS             complement(<1..426)
                     /locus_tag="P5138_07020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRV60366.1"
                     /translation="MTVSVVTGGAGFVGSHLCDALLEKGNKVICIDNLETGSLQNIEH
                     ITSDDFTFLPQDITEEVHVEGDVDFVYHLASPASPIDYARLPLHTLKVGAQGTWHMLG
                     VAKFKRARFLLASTSEVYGDPQVHPQEESYWGHVNPIGPR"
     gene            514..1521
                     /locus_tag="P5138_07025"
     CDS             514..1521
                     /locus_tag="P5138_07025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRV60367.1"
                     /translation="MEAIRRKAIRISLIAVLAVFGLGTAGSAAASWQSAPVVPVLSAK
                     DQARLKSRVAAGRKSGLRRGVFAKVGDSNTEFSPDLYGLACGSPTGLTASLRATLRAY
                     NRVRLQNPRALPGCRPWTSFSRRSSSAQAGVFSTWTVTPVKNLPDTGYWAKPPGCPLE
                     GTPVDCELDTLNPRYAVVLLGTNDLGMDLAFGKAPGSQIVPRLASVIRALRARKVVPV
                     LSTIPPIIQTDSDRQAVFNAGVARTNRGIWKLSKAWHLPMMNLWRACEQPFMINQGLS
                     VDGLHLGVAGADGAMVGVQPGETTFADSVDFSPGSLRYGANRRNLIWLKTLARLDRIT
                     G"
     gene            1523..>2255
                     /locus_tag="P5138_07030"
     CDS             1523..>2255
                     /locus_tag="P5138_07030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRV60368.1"
                     /translation="MLRPLLALLAAGLLLAGCGSGSNSPSSDQEPGSSPQPSVDVPST
                     QSEPVLPDLSGAAGERLRQEVARADEEKMNPEVFAKVGDSNTEWPQNIYGLGCREVGY
                     GSNGDLRTVVARYSKVKFPDLAGLPGCSPVNSLTRRSAAAVSGVWTEWLMTPTADLPA
                     TGIAPPTDECEPQQTPLECEIELIRPRWALIMSGTNDALLGLPLGATYKRELGKMIDR
                     VRQLGPVPVLSTLPPMAVPAHNGQPG"
BASE COUNT          353 a          760 c          749 g          393 t
ORIGIN      
        1 gcgcgggcca atcggattga catggcccca gtagctctcc tcctgggggt ggacctgggg
       61 gtcaccgtaa acctcggagg tggaggcgag cagaaaccgg gcccgcttga acttggccac
      121 gccgagcatg tgccaggtgc cctgcgcacc gaccttgagg gtgtggaggg gcagccgtgc
      181 gtagtcgatc gggctggccg gggaggcgag gtggtaaacg aagtcgacgt cgccctcgac
      241 atggacctcc tcggtgatgt cctggggcag gaaggtgaag tcgtcgctcg tgatgtgctc
      301 gatgttctgg agcgagccgg tttccaggtt gtcgatgcag atcaccttgt tgcccttttc
      361 gagcagggcg tcgcagaggt gcgatcccac gaatccggct ccgccggtta ccacgcttac
      421 ggtcatggcg ctgatcgtag tcgaggtccc gcgggcagtg ccgctccggt ccactatcgt
      481 ttcctgactg tcattcgcag gggaatggaa gccatggaag caatcaggag aaaagcgatc
      541 cggatttcgc tgatcgccgt tctggccgtc tttggtctcg ggacggcagg ttcggctgcc
      601 gcctcctggc agagcgcccc ggtggtgccg gttctctcgg ccaaggacca ggccaggctg
      661 aagtcccggg tcgcggccgg ccggaaatcc ggtctgcgcc ggggagtgtt cgccaaggtc
      721 ggcgattcga acaccgagtt ctcccccgac ctctacggcc tcgcctgcgg ttccccgacc
      781 gggctcacgg cttccctgcg ggcgaccctg agggcctaca acagggttcg cctgcagaat
      841 ccccgggcgc ttccaggctg ccggccctgg acctcgtttt cccggcgttc ctcgtccgcc
      901 caggccggag tgttctcgac ctggacggtc acgccggtga agaaccttcc ggacaccggc
      961 tactgggcaa agcccccggg ctgcccgctc gagggcacgc cggttgactg tgaactggac
     1021 actttaaatc cgaggtatgc cgtggtcctg ctcggcacca acgacctcgg gatggacctc
     1081 gccttcggca aggcgccggg cagccagatc gttccccggc tcgcctcggt catcagggcc
     1141 ctgcgggccc ggaaggtagt cccggtgctc agcaccatcc cgccgatcat ccagacagac
     1201 agtgaccggc aggccgtttt caacgccggg gtcgcccgca cgaaccgcgg catctggaag
     1261 ctgtccaagg cctggcacct gccgatgatg aacctctggc gggcctgcga gcagcccttc
     1321 atgatcaacc agggtctttc cgtggacggc cttcatctcg gggtcgccgg tgccgacggc
     1381 gccatggtcg gggtgcagcc gggggaaacg accttcgccg attcggtcga tttcagcccg
     1441 ggctctctcc ggtacggagc caaccgccgc aacctgatct ggctgaagac cctggcccgg
     1501 ctcgaccgga tcaccggctg agatgctccg accgctgctt gccctgctcg ccgccgggct
     1561 gcttctcgca ggttgcgggt cgggttcgaa ctcgccttcg agcgaccagg agcccggctc
     1621 ctcaccgcag ccgtccgtcg atgttccctc cacccaaagc gagcccgtgc tgccggatct
     1681 ttcgggcgcc gccggcgagc gactccgaca ggaggtggcc cgcgccgacg aagagaagat
     1741 gaaccccgag gtcttcgcca aggtcggtga ctcgaacacc gaatggccgc agaacatcta
     1801 cgggctgggg tgccgggagg tcggctacgg atcgaacggt gacctcagga cggtggtcgc
     1861 ccggtattcg aaggtgaagt tccccgacct ggccggactg cccggctgct ctccggtcaa
     1921 ttcgctcacc cgccgctccg ccgctgcggt ctccggggtc tggaccgagt ggctgatgac
     1981 cccgaccgcg gacctcccgg ccaccggtat cgccccgccg accgatgaat gcgaaccaca
     2041 gcagaccccg ctggaatgcg agatcgagtt gatcagaccg cgttgggcgc tgatcatgtc
     2101 gggaaccaac gacgccctgc tcgggctgcc actcggcgct acttacaagc gggaactggg
     2161 gaagatgatc gaccgggtgc ggcagctcgg gccggtgccc gtcctctcga ccctgccgcc
     2221 gatggcggtt cccgcgcata acggccagcc cggcg
//
LOCUS       DAOTIP010000342         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_56987_length_2254_cov_3.203729, whole genome shotgun sequence.
ACCESSION   DAOTIP010000342 DAOTIP010000000
VERSION     DAOTIP010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56987_length_2254_cov_3.203729"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1026)
                     /locus_tag="P5138_07035"
     CDS             complement(79..1026)
                     /locus_tag="P5138_07035"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRV60369.1"
                     /translation="MKHLLSIEDLDQEAVESLLDRAESFAEVSKRDIKKVPALRGRTV
                     VTLFYEASTRTSSSFELAAKRLSADVVSVKASGSSVEKGESLKDTVETLSAYDPASII
                     IRHPQAGAAQMLSGWVDAAIVNAGDGMHQHPSQALLDLFALRQRIGSIEGRKIWIVGD
                     VLHSRVARSNIQAFSMMGAEVTIAGPPTLIPRGIEASGCEVRYSLDGIEEADVVYLLR
                     MQRERMEESFVPSIREYARLFQVNSRRLNPRQLVMHPGPVNRGVEIGGDVIDSDRCLI
                     ADQVASGLAVRMAILFELLAHGGETHASAEGPVPIGEPA"
     gene            complement(1023..1577)
                     /gene="pyrR"
                     /locus_tag="P5138_07040"
     CDS             complement(1023..1577)
                     /gene="pyrR"
                     /locus_tag="P5138_07040"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRV60370.1"
                     /translation="MNEKVVLEANDVERTLRRIAHEIVEKNPDVSDLAIVGIHTGGAY
                     LAERLTSMIAGLSGEAPPLGEIDISFYRDDIGIRGSDYQPVVHSTRLDFPLEERTVVL
                     VDDVLFTGRTVRAAIEALFDFGRPARIQLAVLCDRGHRELPIRPDYVGKNLPTSRSEQ
                     VSVRLKETDGEDEVAILSPVEAVE"
     gene            complement(1871..>2254)
                     /locus_tag="P5138_07045"
     CDS             complement(1871..>2254)
                     /locus_tag="P5138_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV60371.1"
                     /translation="DRLGRAVAITGDTRPSRNTVEAAHDADLLIHDASFMVEDRDRAD
                     ETGHSTGTEAAEVAAEAGAKMLALVHISSRYHVGKALEEAKAVFENTVAPRDFDTVEI
                     PFPERGEPELVPKGAKRNAPPSDPE"
BASE COUNT          362 a          736 c          756 g          400 t
ORIGIN      
        1 ggcccgggcg ctggaccagg ggcgccggca ggccgccgtt ctccgcgccg tcttcgggca
       61 ggccgtcgat cacctcgttc atgccggctc tccgatcggc accgggccct cggcgctggc
      121 atgggtctcg ccaccgtggg cgagaagttc gaacaggatc gccatccgga ccgcaagccc
      181 cgaggccacc tgatcggcga tcaggcaccg atccgagtcg atcacgtcac cgccgatctc
      241 gaccccgcgg ttgaccggac ccggatgcat gaccagctgc cgggggttga gccgccggga
      301 gttcacctgg aaaagccggg cgtactcgcg aatcgagggc acgaagctct cctccatccg
      361 ttcgcgctgc atcctcagga ggtagacgac gtccgcttcc tcgattccgt cgaggctgta
      421 gcggacctcg cagccggaag cttcgattcc gcggggaatc agggtcggcg ggccggcgat
      481 ggtcacctcc gctcccatca tcgagaaggc ctgaatattc gagcgggcga cccggctgtg
      541 gagcacatcc cccacgatcc agatcttcct gccctcgatc gatccgatcc gctgcctcag
      601 tgcgaagagg tcgagcaacg cctggcttgg atgctggtgc atcccgtccc ccgcgttcac
      661 gatcgcggcg tcgacccagc cactcagcat ctgtgcggca ccggcctgcg ggtggcggat
      721 gatgatcgac gccgggtcgt aggcggaaag ggtctccacc gtgtccttca gggattcacc
      781 cttctcgacc gacgatccgg atgccttgac cgaaaccacg tcggcggaaa gccgcttggc
      841 cgcgagctcg aaggaggatg aggtccgggt gctggcctcg tagaagagtg tgaccacggt
      901 gcgaccacgc agggccggca ccttcttgat gtcccgcttc gagacctcgg cgaagctttc
      961 ggcccggtcg agcaggctct ccaccgcttc ctggtcgagg tcctcgatgc tgagcagatg
     1021 tttcattcga ccgcctccac cggggaaaga atcgccactt cgtcttcgcc atcggtctcc
     1081 ttgagccgca ccgacacctg ttcgctgcgc gaggtcggga ggttcttgcc gacgtaatcg
     1141 ggccggatcg gaagttcgcg gtggccgcgg tcgcagagca cggccagctg aatccgggcg
     1201 ggccggccaa agtcgaagag cgcctcgatc gcggcccgca ccgtccggcc ggtgaaaagg
     1261 acgtcgtcga ccagcaccac ggttctctcc tcgagcggaa agtcgagtcg ggtcgaatgg
     1321 accaccggct ggtagtcgga gccccggatg ccgatgtcat cgcggtagaa ggagatgtcg
     1381 atctccccga ggggaggcgc ttcaccggac aggccggcga tcatcgaagt gaggcgttcg
     1441 gcgagatagg cgccgccggt gtgaatgccg acgatggcga gatcggagac gtccggattc
     1501 ttctcgacga tctcgtgggc gatgcgacgg agcgtgcgct cgacgtcgtt tgcttccagc
     1561 acaaccttct cgttcaaagt caccttcccg gcctcacagg acctgggtta aaggaacggg
     1621 gatgaagata gcagccgccc ccgatgttgg ccgtcgtgta cccgcaccgg acccgagaga
     1681 acttcggtgg gcccggcacc ggcatgcccc tcctgggccg tcgccctgcg ggctcccccg
     1741 aaggcccatg aggggcatgc cggcaccgag ccaacccaga ctctcttagg tccggcgcgg
     1801 gtatagccgg aaaagacctt cgggggagcg cgaagcgcga cgggcttttc cggctatacc
     1861 cgtgacgggc ctactccggg tcagatggcg gcgcgttgcg ctttgctccc ttggggacca
     1921 gttctggttc gccgcgctcg gggaagggga tttctactgt gtcgaagtcg cgcggggcca
     1981 ccgtgttctc gaagacggct ttggcttctt caagggcctt gccgacgtgg tagcgggatg
     2041 agatgtggac cagggcgagc atcttggcgc ccgcttcggc agccacttcg gcagcctcgg
     2101 ttccggtcga atggccggtt tcgtcggccc ggtcgcggtc ctcgaccatg aagctcgcgt
     2161 cgtggatcag caggtccgcg tcatgtgccg cttcgactgt gtttcgggaa ggccgggtgt
     2221 cgccggtgat cgcgacggca cgcccgagcc ggtc
//
LOCUS       DAOTIP010000343         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57133_length_2250_cov_2.553531, whole genome shotgun sequence.
ACCESSION   DAOTIP010000343 DAOTIP010000000
VERSION     DAOTIP010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57133_length_2250_cov_2.553531"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /gene="ispH"
                     /locus_tag="P5138_07050"
                     /gene_synonym="lytB"
     CDS             <1..262
                     /gene="ispH"
                     /locus_tag="P5138_07050"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574867.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRV60372.1"
                     /translation="VEIAREHGSESFLIDNHTQVEEEWLDGVETVGITSGASAPEELV
                     EDLVQLFRDRGVEDVSELRTVDESVRFMLPKEIRKGLPMVNS"
     gene            complement(269..1120)
                     /locus_tag="P5138_07055"
     CDS             complement(269..1120)
                     /locus_tag="P5138_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60373.1"
                     /translation="MTEGGSYPYDHAILPLRDDIAAPELPGKATWIGKDPGKMPVLTA
                     KGPVLVHFLDYSQLNSVRTLPYLVEWHERYEPLGLRVLGVQAPRFPFGADPEAAGRGL
                     RQLGVEFPVLIDESKRLWTGYGCEGWPSLFLWGRGGILRWFHFGEGDYQGTEQAIQEA
                     LAASFERLPEMPAIMEPVRPTDAAGVEVSPPSPEVIPFPDRPLTIDDFDALALDFEGG
                     GAYVTADGKGDLRVTLDGEARGEVGIDGPGLYPLVETGRHGSHKLGIELEGAPEIWSV
                     SYSPAAT"
     gene            1153..1782
                     /locus_tag="P5138_07060"
     CDS             1153..1782
                     /locus_tag="P5138_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRV60374.1"
                     /translation="MNTNLWDRIEQARAEHNVLEHPFYQRWSAGELTREELADYSGQY
                     RYATEAIAKLSTDIAAQAPESERAGLEAHAAEEHDHVVMWDGFVGAVGGTVGAEPNPE
                     TLACVEAWTKADGFGRQLARMFAIESGQPAISKTKTEGLGEHYEITDSPGNLYFTVHE
                     EMDVHHAAEGRKLIERYMNDFSEDELVEAAEEAFKANWLLLDGVEKARA"
     gene            1938..>2250
                     /locus_tag="P5138_07065"
     CDS             1938..>2250
                     /locus_tag="P5138_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60375.1"
                     /translation="MAPGFGRAITSLPVRLLCGAFGLFLLGVAIYSGFKGTEAPDRNV
                     TLTLLFVTAWLGFPLLGVLVGNLFPAFNPWNAVAAPAGWAWRRVAGKAPAHLAYPERL
                     GR"
BASE COUNT          376 a          749 c          739 g          386 t
ORIGIN      
        1 ggtcgagatc gcgcgcgagc acggctccga atccttcctg atcgacaacc acacccaggt
       61 tgaggaagag tggctcgacg gggtggaaac ggtcgggatc acttccgggg caagcgcacc
      121 ggaggaactg gtcgaggatc tggttcagct tttccgcgac cgcggggtgg aggacgtctc
      181 cgagctccgc accgttgacg agtcggtccg cttcatgctg ccgaaggaga tccgcaaggg
      241 cctcccgatg gtcaattcct gaccggtctc aggtcgcggc cggcgagtaa ctgaccgacc
      301 agatctccgg agcgccttcc agttcgatcc ccagcttgtg gctgccgtgg cggccggttt
      361 cgacgagcgg gtaaagcccc gggccatcga tcccgacttc gccgcgggct tcgccgtcga
      421 gggtaacccg cagatctccc ttgccatcgg ccgtgacgta ggcgccaccg ccctcgaaat
      481 cgagcgccag cgcgtcgaag tcatcgatcg tgagggggcg gtccggaaac gggatcactt
      541 cgggtgaggg tggcgacacc tcaacgcccg ctgcgtcggt gggccggacc ggttccatga
      601 tcgccggcat ctccgggagc ctctcgaagg aggcggccag cgcttcctga atcgcctgct
      661 cggttccctg atagtcgccc tcgccgaagt ggaaccagcg gaggattccc ccgcgacccc
      721 agaggaagag gctcggccag ccttcacatc cgtaaccggt ccagagtcgc ttgctttcat
      781 cgatcaggac cgggaactcg accccgagtt gccgcagtcc gcgcccggcg gcttccgggt
      841 cggcgccgaa gggaaagcgt ggagcctgga caccgagcac ccgcaggccg agcggctcgt
      901 aacgctcgtg ccactcgacc aggtagggca gggtgcgcac gctgttcagc tggctgtaat
      961 ccagaaagtg gaccagtaca gggccttttg ccgtgagcac cggcatcttg cccgggtcct
     1021 tgccgatcca ggtcgccttg ccgggcagtt cgggagccgc gatgtcatct cgaagaggga
     1081 ggatggcgtg atcgtatgga tatgatccgc cttcagtcac cccctctacg agctctacga
     1141 ggagagaaac tcgtgaacac gaatctgtgg gaccgcatcg agcaggcccg ggccgagcac
     1201 aacgtcctgg aacatccctt ctaccagcgc tggtcggccg gcgagctgac ccgcgaggaa
     1261 ctcgccgatt actcgggcca gtaccgctac gcgaccgagg cgatcgccaa gctgagcacc
     1321 gacatcgccg cccaggcacc ggaatccgag cgggccgggc tcgaggcgca tgcggccgaa
     1381 gagcacgatc acgtcgtcat gtgggacggc ttcgtgggcg cggtcggcgg cacggtaggt
     1441 gccgagccga acccggagac cctcgcctgc gtcgaagcct ggaccaaggc cgacggtttc
     1501 ggccgccagc tcgcgcgcat gttcgcgatc gagtcgggcc agccggcgat ttcaaagacc
     1561 aagaccgaag gtctcggcga gcactatgag atcaccgact ctcccggcaa cctctacttc
     1621 accgttcacg aggagatgga cgtccatcac gccgccgagg gtcgcaagct gatcgagcgg
     1681 tacatgaacg atttcagtga ggacgagctg gtcgaggcgg ccgaggaagc cttcaaggcc
     1741 aactggctgc tgctcgacgg agtcgagaag gcgcgggcct gagccttccc ctcgcacatg
     1801 tcctgtccgc cgggcaggac ctgccaatac cggcgtggct cttcgcctgg ggagcctcga
     1861 ttgttctcgt agtctcgttc ttcgctctct cagcgggctg gcgccgggcc cgctacgaga
     1921 gtgattcctg gcgtcaggtg gcgcccggtt tcggccgcgc gatcaccagc ctgccggtcc
     1981 ggctcctctg cggggccttc ggcctcttcc tgctcggggt cgcgatctat tccggcttca
     2041 aaggcaccga ggcgccggac cgaaatgtca ccctgaccct gcttttcgtc accgcctggc
     2101 tcggctttcc gttgctggga gtgctggtcg ggaacctctt tcccgccttc aacccatgga
     2161 acgcggtcgc cgccccggcc ggctgggcct ggcgcagggt cgcgggcaag gctcctgccc
     2221 accttgccta tccggagcgg ctcggacgct
//
LOCUS       DAOTIP010000344         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57291_length_2245_cov_2.611872, whole genome shotgun sequence.
ACCESSION   DAOTIP010000344 DAOTIP010000000
VERSION     DAOTIP010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57291_length_2245_cov_2.611872"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1433
                     /locus_tag="P5138_07070"
     CDS             210..1433
                     /locus_tag="P5138_07070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRV60376.1"
                     /translation="MSESDSKRVVVIGSSFAGLTGALQLRKLLDKKHEVVVLDPRDHF
                     TFIPSLIWLPFGARKAEDVTFKLEPMYAKKGIRFINEAAAAIDTDAHTVTTTSGEEID
                     YDRVLVATGPRLAFERIQGLGPEGGYTESICNLEHAEKAAEGWQKFLENPGPVVVGTA
                     QGGSCFGASYEFLFNVKHRIKKAGLEDKAPVTFVTAEPYLGHFGLAGVGDSSDMVGKF
                     FDKNDIQGLPNSVITEVRDGEIDLESGETLPFAYSMIVPPFGGVDVVTGTPGLANDAG
                     FIPVDSYFRSTNLPEVFSAGVSIAVAPPEKTQVPAGVPKTGQMSETMAKVAAENIVAD
                     LEGRARWEMPMDELAAVCVLDAGNSGIIFKAEHVLDKSQEADAHLMAGPQAHWAKLAF
                     ERIFISSRKRGHIVL"
     gene            complement(1472..1921)
                     /locus_tag="P5138_07075"
     CDS             complement(1472..1921)
                     /locus_tag="P5138_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60377.1"
                     /translation="MPGGLPFIDEHSVEIEAEIQTVWDSLIRSFGSATGGGLFALYGR
                     AIGVEPLNRNGVLDEIGSTQIGFRVSEAFAPELLVLRGRHRFSNYSLTWRLAPGDHAA
                     TRLSAITHAEFPGIQGRIYKALVIDSGTHGRVTRRMLSSIARQSTRT"
     gene            complement(1977..>2245)
                     /locus_tag="P5138_07080"
     CDS             complement(1977..>2245)
                     /locus_tag="P5138_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60378.1"
                     /translation="TAQVGAAIGLAVLATISANKTDALLAAGDPRREALTGGYEIAFW
                     VAVGLVVAALAVAFIVLEPAPAREVGEDPGEVTELELETEPGTG"
BASE COUNT          426 a          696 c          719 g          404 t
ORIGIN      
        1 agcggggcgg actccagatc ggaatcggac agacgctcga gagtgtcggg agattcggca
       61 tatgccggtg gacttgtggc cacggcggga tggtataccg aacggatctc ggttagacct
      121 ggtcgtatgc tggttcaggg ttttcccgca accggtctat accactcacc agtagtatag
      181 atgtattcgc caccgaccag gagaatgaaa tgtccgagag tgattcgaag cgcgtcgtag
      241 taatcggctc gtcctttgcc ggcctgaccg gtgcgcttca gctgagaaaa ctgcttgaca
      301 agaagcatga ggttgtggtg ctcgaccctc gagaccactt caccttcatc ccctcgctta
      361 tctggcttcc cttcggggcg cggaaagccg aagacgtgac cttcaagctg gaaccgatgt
      421 acgccaagaa ggggatccgg ttcatcaacg aagcggcggc cgcgatcgat actgacgcgc
      481 acacggtgac aaccacttcg ggtgaggaaa tcgattacga ccgcgtactc gtggcgaccg
      541 gaccgaggct cgccttcgag cggatccagg gtctgggtcc ggagggcgga tacaccgagt
      601 cgatctgcaa tctcgagcat gccgagaagg cggccgaggg ctggcagaag ttcctcgaga
      661 acccgggtcc ggtggtcgtt ggcacggccc agggcgggtc ctgcttcggt gcctcatacg
      721 aattcctttt caacgtcaag caccggatca agaaggccgg cctcgaagac aaggccccgg
      781 tcaccttcgt cacggcagag ccctatctgg gccacttcgg cctggccgga gtcggtgact
      841 cgtccgacat ggtcgggaag ttctttgaca agaacgacat ccagggcctg ccgaattcgg
      901 tgatcaccga ggtccgcgac ggcgagatcg acctggagtc cggagagacc ctgccctttg
      961 cctactcgat gatcgtgccg cccttcggcg gagtcgacgt ggtcaccgga acacccggcc
     1021 tggccaacga cgccggcttc attccggtcg acagctactt ccggagcacc aaccttcccg
     1081 aggtgttctc ggccggagtg tcgatcgctg tggcgccgcc ggagaagacc caggttccgg
     1141 ctggtgtgcc caagaccggt cagatgagcg agacgatggc caaggttgcc gccgagaaca
     1201 tcgtcgccga cctcgagggc cgtgcgcgct gggagatgcc gatggacgaa ctggccgcgg
     1261 tctgcgtcct cgacgccggc aacagcggga tcatcttcaa ggccgaacat gtcctcgaca
     1321 agtcacagga agcggacgct cacctcatgg cgggacccca ggctcactgg gcgaagctgg
     1381 ccttcgagcg catcttcatc tcgagccgga agcgcgggca catcgttctc tagagcgttg
     1441 tgcctacttc cgcctcgagc ccggatctct cctaggtccg ggttgactgt cgtgcgatag
     1501 aggagagcat ccggcgcgtg acccgcccgt gggtgccgga gtcgattacc agcgccttgt
     1561 agatgcggcc ttgaatcccg ggaaactctg catgtgtgat cgccgaaagc cgggtggccg
     1621 catggtcacc cggtgccagt ctccaggtca gggagtagtt cgaaaagcgg tgcctgccgc
     1681 gcaggaccag caactcgggg gcgaaagcct cggaaacccg gaagccaatc tgggtcgaac
     1741 caatctcgtc gagtaccccg ttcctgttca gtggctcgac cccgatcgct ctgccgtaga
     1801 gcgcgaagag gccgcctccg gttgcgcttc cgaacgaacg gatgagggag tcccagacgg
     1861 tttggatttc cgcctcgatc tcgaccgaat gctcgtctat gaaaggcagt ccgccaggca
     1921 tgccccgatg ctagccccgg catgcgaccg ggcatgtgac agtcgcactc gtgcggttac
     1981 ccggtgccgg gctcggtttc gagttcgagc tcagtcactt caccgggatc ttcaccgacc
     2041 tcccgggcgg gggccggttc cagcacgatg aaggcaacgg ccagcgccgc cacgaccagg
     2101 ccgacggcga cccagaaggc gatttcataa ccaccggtca aggcttctcg ccgcggatcg
     2161 cccgcggcga ggagcgcatc ggtcttgttg gccgagatcg tggcgaggac cgccaatccg
     2221 atcgcggcgc cgacctgcgc ggtcg
//
LOCUS       DAOTIP010000345         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57314_length_2244_cov_3.262220, whole genome shotgun sequence.
ACCESSION   DAOTIP010000345 DAOTIP010000000
VERSION     DAOTIP010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57314_length_2244_cov_3.262220"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2227)
                     /locus_tag="P5138_07085"
     CDS             complement(<1..2227)
                     /locus_tag="P5138_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60379.1"
                     /translation="MEADSIPGSNSGANGAGYAPSPLSTDGRFVLFTSMSDNLVSDND
                     LNTTGLFRRDMQNGEIVLVSRLNGKNGAPIKGGFYDFKMDGSGNRVVFTTDQQLTGDD
                     TDDQPDAYMRDIAAGTTTLVSPDTSLDVINPAISADGQYITFSTLSALVPGDLNGKYD
                     VYRRKVSDGTVGIVSRIPAAATAGDSDSFSSGISGDGRWVAFSSRATNLVAGFVENNS
                     AFATDVFVRDMVSGNTYLVSSRFNSSLEGANDGSEDPVIAGTPGTLNTVRVAFTSYST
                     NLADNGVSDSDTAASVYLKSFPSQASVLVSRASGATGENANSRAHTPSISDNASRIVF
                     TSDAGNLGAGDNYYGTYLRNLTDNTTTLVSNDNSYAVWGQISGNGATASWDEAGGGTP
                     DSDPDLGCVFRRTFSGDIVRLVSRPKGSKKVAAPGFASYSGHDSKRALSADGRYMVFA
                     TWSSHLPSSEIFQQQIYRRDLKTGKIEVVSRASGANGALAEVGFAPSISGNGNLVAFT
                     AAGQLDPADTNDRSDIYVRNMSTGTTTLVSRADGPDGAVSDEGGHYPSISTDGTRVAY
                     SSEASNMGAPGGHEIVYVRDLGSGTTSIASRATGLAGAIANQYSSDPMMNGDGSLVVF
                     GSNATNLSPDDGASSNSIYLRNIDTGETLLVSRNPGLTGTSLPNYVYDGAINSDGTKV
                     AFVTANDTAVPATAPWPAGQGQVVIRTIADGANSLGSSTPDGVVGNDRSDSPSFSGDG
                     GI"
BASE COUNT          350 a          682 c          753 g          459 t
ORIGIN      
        1 ggatcccccc gtcgccgctg aagctcgggc tgtccgagcg atcgttgccg accacgccgt
       61 ctggcgtgga actgccgagc gagttcgcgc cgtcggcgat cgtccgaatt accacctgac
      121 cctggccggc cggccagggg gcggtggcgg gtacggcagt gtcatttgcc gtgacgaaag
      181 cgaccttggt cccgtccgag ttgatcgctc cgtcgtagac gtagttgggc agagacgttc
      241 cggtcaggcc ggggttgcgt gaaaccagca acgtttcgcc ggtgtcgatg tttcgcaggt
      301 agatgctgtt ggaactggcc ccgtcgtcgg gcgacaggtt cgtggcgttt gatccgaaca
      361 cgaccagcga tccgtccccg ttcatcatcg ggtcgctcga atactggttg gcgatcgcgc
      421 cggccagtcc cgtagccctc gaggcgatcg aggtcgtccc ggaaccgagg tcccttacgt
      481 agacgatctc gtgtccgccc ggggcaccca tgttcgacgc ttccgaggag taggccactc
      541 tcgttccgtc ggtgctgatc gacgggtagt ggccaccctc gtcagatacc gctccgtccg
      601 ggccgtcggc ccgggagacc agcgtcgtcg ttccggtgct catgttgcgg acatagatgt
      661 cggagcggtc gttggtgtcg gccgggtcaa gctgtccggc tgcggtgaag gccacgagat
      721 tgccattccc gcttatcgaa ggcgcgaacc ccacttcggc aagcgctccg ttcgcgccgc
      781 ttgcccgcga gacaacctcg atcttcccgg tcttcaggtc ccgcctgtag atctgctgct
      841 ggaagatctc gctactcggc aggtggctcg accaggtagc gaagaccatg taacgcccgt
      901 cggcgctgag tgcccgcttc gagtcgtggc ccgagtagct ggcgaacccg ggggccgcga
      961 ctttcttgct tcccttcggg cgggaaacca gtcgcactat gtccccggag aaggtccgcc
     1021 ggaagacgca gccgaggtca gggtcgctgt caggagtgcc cccgcccgct tcgtcccagg
     1081 aggctgtcgc gccgttgccg gaaatctgtc cccaaaccgc gtaactgttg tcattcgaca
     1141 cgagagtcgt ggtgttgtcg gtcaggttgc ggaggtaggt cccgtagtag ttgtctccgg
     1201 cgcccaggtt gccggcgtcg gaggtgaaga cgatgcggga agcgttgtcg ctgatgctcg
     1261 gggtgtgggc ccggctgttc gcgttttccc cggttgctcc gcttgcccgg ctgaccagta
     1321 cggatgcctg ggacgggaaa gacttcaggt agaccgaggc ggcggtgtcg gaatcggaaa
     1381 ccccattgtc ggccaggttc gtcgagtagc tggtgaaagc cacccggacc gtgttcaggg
     1441 tgcctggggt gcctgctatc accggatctt ctgatccgtc gttggccccc tccaggctcg
     1501 agttgaagcg ggaactgacc agatatgtgt tgccgctgac catgtcacgc acgaagacat
     1561 cggtcgcgaa cgcggagttg ttctcgacga aaccggctac caggttggtt gctctcgagc
     1621 tgaacgcgac ccagcgaccg tcgccgctga tcccgctgga gaaggagtcg ctgtcgccgg
     1681 cggtggccgc ggccgggatg cgcgaaacga tgccgacggt cccgtccgag actttgcgac
     1741 ggtagacgtc gtacttcccg ttcaggtcac ctggcaccag tgcgctcaac gtggagaagg
     1801 tgatgtactg accgtcggct gaaatggccg gattaatcac gtcgaggctg gtgtccgggg
     1861 acaccagggt cgtggttccg gcggcgatgt cccgcatgta tgcatccggt tggtcgtcgg
     1921 tgtcgtctcc ggtcagttgc tggtcggtgg tgaagaccac gcggttgccg cttccgtcca
     1981 tcttgaagtc gtagaaaccg cccttgatcg gcgcaccgtt ctttccgttc agccgagaga
     2041 ccagcacgat ctccccgttc tgcatgtctc ggcggaaaag cccggtggtg ttgaggtcgt
     2101 tgtcgctgac caggttgtcc gacatcgagg tgaagaggac aaaccgcccg tcggtgctca
     2161 ggggtgatgg tgcgtatccc gccccgttgg ccccggaatt gctgccggga atcgagtcag
     2221 cttccatcgg aagccagctg acgg
//
LOCUS       DAOTIP010000346         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57353_length_2243_cov_3.337751, whole genome shotgun sequence.
ACCESSION   DAOTIP010000346 DAOTIP010000000
VERSION     DAOTIP010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57353_length_2243_cov_3.337751"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /gene="scpA"
                     /locus_tag="P5138_07090"
     CDS             complement(<1..1685)
                     /gene="scpA"
                     /locus_tag="P5138_07090"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRV60380.1"
                     /translation="MSGIPDFSEIELGPVQSGPADNAELEAILEQHAGADGDEVRWET
                     PEGITLDAAYTSAALEGIDFLDTKPGFPPFLRGPYPSMYVNKPWTIRQYAGYSTAEES
                     NAFYRRNLAAGQKGLSIAFDLATHRGYDSDHERVAGDVGMAGVAIDSIYDMRTLFEGI
                     PLEEMSVSMTMNGAVLPVLSLFITAAEEQGVPHDQITGTIQNDILKEFMVRNTYIYPP
                     EQSMRIVSDIIGYTSKELPRFNSISISGYHMQEAGASADLELGYTLSDGVEYVKAALD
                     SGLEIDQFAPRLSFFWAIGMNFFMEVAKMRAGRLLWASLIKEFGPQKERSMSLRTHCQ
                     TSGWSLAAQDVYNNVVRTCVEAMAATQGHTQSLHTNGLDEALGLPTDRTARIARQTQL
                     FLQQESGTTRVIDPWAGSYYVEYLTRQLALKALGHMKEIDEAGGMPEAIAKGIPKLRI
                     EESAARTQARIDSGKQTVVGVNSYKPENDAIVNVLKIDNNEVRAAQLAKLERLRAERD
                     EAAVQEALEHLTNAARSGEGNLLDLGVKAARVYATVGEISYALEKEYGRHQAE"
     gene            complement(1682..>2243)
                     /locus_tag="P5138_07095"
     CDS             complement(1682..>2243)
                     /locus_tag="P5138_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60381.1"
                     /translation="DGGTDGRGTIEARIDELVPTRAEEPFSLPALNPHPDSTPFELMR
                     RAAADHEKAGGDRPQIYLACMGTIASHVNYANWAKSFFEVAGVETVPSGALESNEEQA
                     KTLRGGGFELVAVCAGRNQTPEDVADLVTRLRDAGAQYIYMVNSTPELNEAAMDAGAD
                     ELVKNGVNLTGVLTATLGRLGVEVKS"
BASE COUNT          347 a          717 c          742 g          437 t
ORIGIN      
        1 atctcggcct ggtgacgacc gtactccttc tcgagcgcgt agctgatttc gccgaccgtg
       61 gcatagacac gtgcggcctt cacaccgagg tcgaggaggt tgccctcgcc gctcctggcc
      121 gcgttggtca ggtgctccag ggcctcctgt acggctgcct cgtcacgctc ggcccgcagc
      181 cgttcgagct tggccagctg ggcggcacga acctcgttgt tgtcgatctt cagcacgttg
      241 acgatcgcgt cgttttcggg cttgtaggag ttgacgccga ccacggtctg cttgccggag
      301 tcaatccggg cctgggtccg ggcggccgac tcctcgatcc gcagcttcgg gatgcccttg
      361 gcgatcgctt cgggcatgcc gccggcctcg tcgatctcct tcatgtgacc cagtgccttc
      421 agcgcgagct gcctggtcag gtactcgacg tagtaactgc cggcccaggg gtcgatcacc
      481 cgggtagtac ccgattcctg ctggaggaag agctgggtct ggcgggcgat ccgggcggtc
      541 cggtcggtcg gcagcccgag cgcttcatcg agcccgttgg tgtggagcga ctgcgtgtgg
      601 ccctgggtgg cagccatcgc ctcgacacag gtccgaacca cgttgttgta gacgtcctgg
      661 gcggcgagac tccagcccga ggtctggcag tgggtgcgga gcgacatcga acgctccttc
      721 tgcgggccga actccttgat caacgacgcc cagagcaggc ggccggcccg catcttcgcg
      781 acctccatga agaagttcat gccgatcgcc cagaagaagg aaagccgcgg tgcgaactga
      841 tcgatctcga ggccggaatc gagagccgcc ttcacgtact cgaccccgtc cgagagtgtg
      901 tagccgagct cgaggtcggc gctcgccccg gcctcctgca tgtggtaacc ggaaatcgag
      961 atcgagttga agcggggcag ttccttcgag gtgtatccga tgatgtcgga gacgatccgc
     1021 atggattgct cgggcggata gatgtaggtg ttccgcacca tgaattcctt gaggatgtcg
     1081 ttctggatcg tgccggtgat ctggtcatgc ggcacgccct gctcctcggc cgcggtgatg
     1141 aacagcgaaa ggaccggcag caccgccccg ttcatggtca tcgagaccga catctcctcg
     1201 agcgggatcc cctcgaagag ggtgcgcatg tcatagatcg agtcgatcgc gaccccggcc
     1261 atcccgacgt cgcccgctac ccgctcgtga tccgagtcat agccacggtg agtggccagg
     1321 tcgaaggcaa tcgaaaggcc cttctggccg gccgccaggt tgcggcggta gaaggcgttc
     1381 gattcctctg ccgtggagta gcccgcgtac tgccggatcg tccagggctt gttcacatac
     1441 atcgacgggt acggcccgcg caggaacggc gggaatcccg gcttggtgtc gagaaagtcg
     1501 atcccttcga gggcggcgga agtgtaggcc gcgtcgaggg tgatgccctc cggggtctcc
     1561 cagcgcacct cgtcaccgtc ggctccggcg tgctgttcga ggatcgcctc aagttccgcg
     1621 ttgtcggccg gaccggactg gaccgggccc agttcgatct cggagaaatc cggaatgccg
     1681 ctcatgactt cacctccacg ccgagccggc cgagtgtggc cgtgagaacc ccggtcaggt
     1741 tgacgccgtt cttcaccagt tcgtcggcac cggcgtccat cgcggcctcg ttcagctcgg
     1801 gagtcgaatt gaccatgtag atgtactgcg ctccggcgtc gcggagccgg gtgaccaggt
     1861 cggcgacgtc ttcgggggtc tggttgcggc cggcgcagac cgcgaccagc tcgaagccgc
     1921 ctccacgcag ggtcttcgct tgctcctcgt tgctctccag cgcgccgctc gggacggtct
     1981 cgaccccggc cacttcaaag aagctcttgg cccagttcgc gtagttgacg tggctggcga
     2041 tcgttcccat gcaggcgaga tagatctgtg gccggtctcc gcctgccttt tcgtgatcgg
     2101 ccgcggcccg gcgcatcaac tcgaagggag tcgagtccgg gtgagggttc agggccggca
     2161 gggagaaggg ctcctcggcc ctcgtcggga caagttcgtc gatccgggct tcaatggttc
     2221 ctcgaccatc cgtaccgccg tca
//
LOCUS       DAOTIP010000347         2241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57422_length_2241_cov_4.012809, whole genome shotgun sequence.
ACCESSION   DAOTIP010000347 DAOTIP010000000
VERSION     DAOTIP010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57422_length_2241_cov_4.012809"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1051)
                     /locus_tag="P5138_07100"
     CDS             complement(362..1051)
                     /locus_tag="P5138_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRV60382.1"
                     /translation="MSELVTYKSDSEIATVTMDDGKANAFSLDMLAELHGAIDQAESE
                     GAVIVLRGREGRFSAGFDLKVFQEQPQRIGEMVESGARLAERLLSHSRPVLAACTGHA
                     VAAGAFVLLSCDSRIGIDGPFRIGLNEVAIGLTVPLFVVELARRRLTPAAFDQSVITA
                     AMFDPASAVSAGFLDHAIGAEDFEDAVATEAGRLASLPAEAHAATKGRARNADLEVIR
                     KAIEREMTGNG"
     gene            1142..1768
                     /locus_tag="P5138_07105"
     CDS             1142..1768
                     /locus_tag="P5138_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRV60383.1"
                     /translation="MSQATVTDPESADAPESASGAAENPVGARVKALREAMGFSLRDL
                     SERSGVSPTMLSQVERGSTSPTLAVAEKIAAGLDLSLSQLLRLDEVQHVVVSRSGDHL
                     VRNSKGHRIEELTPPLPGQRADVSTHRLEPGAVTGGADDAPMHEPGSRETAVVIEGEL
                     VLVFEGEPYRLSGGDSVTFDADLPHHFENESDQPVEFIAVIAAGLRRG"
     gene            1785..>2241
                     /locus_tag="P5138_07110"
     CDS             1785..>2241
                     /locus_tag="P5138_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRV60384.1"
                     /translation="MPKTMFEKIWEAHEVRENLLYIDLHLVHEVTSPQAFEGLRMTGR
                     KLRRPDKTVATADHNVPTDGTPAAAMIKDALSRKQVETLEKNAADFGVPVYSLGSETQ
                     GIVHVIGPELGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEGERAK"
BASE COUNT          424 a          697 c          745 g          375 t
ORIGIN      
        1 cgcgagaggt aggggcgggt ttctgtggtc atccggcgca gtcaagattc accgggtccg
       61 ggaggggtcg gataaatgtt cgctaatggg tgactgcggt cacagagtgg acgtatttcc
      121 ctctgtaccc tccatttgca gggttttcct gccacatgaa ccgcaacata agtgagacgt
      181 gatacccatg atgggcaaag ctattttcag cctgctcagc gcctccgctc cggccgacat
      241 gaggaagccg aagcagcccc ggaacgaccg gtaggtgcga agggatccag gatcccgaat
      301 gctttgaaag ccccgcctga gaaggcgggg ctttcttctt gagcggagag ggttccggag
      361 ttcagccgtt gccggtcatt tcgcgctcga tggcttttct gatcacctcg agatcggcgt
      421 tgcgggcgcg ccccttggtg gctgcgtgag cttcggccgg gagggatgcg aggcgacctg
      481 cctcagtcgc cacggcatct tcgaagtctt cagcaccgat cgcgtgatca aggaaccctg
      541 cggaaacggc agatgccggg tcgaacatcg cggcggttat gaccgactgg tcaaacgccg
      601 cgggggtgag gcgccggcgg gccagctcga ccacgaagag gggcacggta aggccgatcg
      661 cgacctcgtt gagcccgatc cggaacggac cgtcgattcc gatccgggaa tcgcaggaaa
      721 gcagcacgaa ggcaccagcc gcgactgcat ggccggtgca tgcggccagg accgggcgag
      781 agtgggagag caggcgctcg gccaggcggg ccccggattc caccatctcc ccgattcgct
      841 gcggctgctc ctggaagacc tttaggtcga agcccgcgga gaacctgccc tcccggcccc
      901 ggagcacaat gacggcccct tcagactcgg cctgatcgat ggccccgtga agctcggcga
      961 gcatgtcgag ggagaaggcg ttggccttgc cgtcgtccat ggtgaccgtc gcgatttcgc
     1021 tgtcgctctt gtacgtgacg agttcactca tccggttcct ttcctcggtc tggctcaacg
     1081 gtattccctg gacccggaaa tgtcttgaca ggacgtatgt gcgtgataac gtacaaccgt
     1141 aatgagtcag gccaccgtca cagatccgga atccgccgac gcacccgagt ccgcctcggg
     1201 cgcagccgaa aacccggtcg gtgcccgggt caaggcactg agggaggcca tgggcttctc
     1261 cctgcgggac ctgtccgagc gaagcggagt aagcccgacc atgctctcgc aggtcgagcg
     1321 cgggagcacc agcccgaccc tggccgtcgc cgagaaaatc gcggccggac tggatctgag
     1381 cctctcgcag ctgctgcgcc tcgatgaggt ccagcacgtg gtggtcagcc gttccggcga
     1441 ccacctggtg cgaaacagca agggtcacag gatcgaggag ctgacgcccc cgttgcccgg
     1501 ccagcgagcc gatgtttcga cccacaggct ggagcctggc gcggtcacgg gaggggcgga
     1561 cgatgccccg atgcacgagc cgggcagccg ggagaccgca gtcgtcatcg agggagagct
     1621 ggtgctcgtc ttcgaggggg agccctaccg cctgtcaggc ggggacagcg tcacctttga
     1681 tgcggatctg ccacaccatt ttgaaaacga aagcgatcag ccggtcgagt tcatagccgt
     1741 gattgcggcg ggactgcgcc gcggatagga aggaaggaga acagatgccg aagaccatgt
     1801 tcgaaaagat ctgggaagcc cacgaggtcc gggagaacct gctctacatc gatctccacc
     1861 tcgttcacga ggtgacttcg ccgcaggcct tcgagggtct gaggatgacc ggccgcaagc
     1921 tgcgtcggcc ggacaagacc gtcgcgacgg cggaccacaa tgtgcccacc gatggcaccc
     1981 cggccgccgc catgatcaag gacgctcttt cccgcaagca ggtcgagacc ctggagaaga
     2041 acgccgccga cttcggcgtg ccggtctaca gcctcgggtc ggagacccag gggatcgtcc
     2101 atgtgatcgg gccggagctg ggcctgaccc agcccggcat gaccatcgtc tgcggtgatt
     2161 cccacacctc gacccacggc gcctttggcg cgctcgcctt cggcatcgga acctcggagg
     2221 tcgaaggcga gcgcgccaaa g
//
LOCUS       DAOTIP010000348         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57489_length_2239_cov_3.501374, whole genome shotgun sequence.
ACCESSION   DAOTIP010000348 DAOTIP010000000
VERSION     DAOTIP010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57489_length_2239_cov_3.501374"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="P5138_07115"
     CDS             complement(<1..1184)
                     /locus_tag="P5138_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60385.1"
                     /translation="MTQLTEDFAIESFADEESVPVEMIEAEQAYRPEILGRVLAVVAA
                     AVALASLDMFIVNIAFPAIEADFPGSSIGSLSWVLNGYVIVFAAMLMPFGRLADRIGR
                     KRVFTTGVLIFCLASGACAAAWSVETLIAFRVVQGVGAAMLMSTSLALLLHAFPAERW
                     PVVIGVWAAAGGAAGALGPPIGGLLVEISWRWVFLVNLPLSLGAWYFGTRLLVESRDR
                     DETRWPDPLGMILMILGIGVLCWGLVEAPDNGWGSAQTLLAILGGVAMLGLTFLRSRR
                     RADRWIPTLDVDLFKARPFAMASLAALVFMVAFAAILLGGVLFLTGVWGYSVLKAGLA
                     FFPGPAMAGIFSVIGSRIGARFGTGPVAATGCVFLILGGLWWFFQMGDVPNYLTENLP
                     GQ"
     gene            1244..1726
                     /locus_tag="P5138_07120"
     CDS             1244..1726
                     /locus_tag="P5138_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV60386.1"
                     /translation="MLDREYSNQVCSIARSLEVVGERWSLLIIRNVGLGMNRFEPLRE
                     NLGITRSVLTTRLNTLIEYGILEKRQYSEKPPRYEYHLTQKGRDLSPVLLQLMWWGDE
                     YYPEPGGPPTIAVHRGSCGGELDHHMVCQKCGESVEPDRVYMKPGPGRGQQDLPKIPA
                     "
     gene            complement(1828..>2239)
                     /locus_tag="P5138_07125"
     CDS             complement(1828..>2239)
                     /locus_tag="P5138_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60387.1"
                     /translation="FLDQYLTNLDSQGATDYAADYAKERIAFGKPINQLQGIQFKLAD
                     METKTAAARELLYKAASMAETNDPKLGKYSAMAKLFASDVAMEVTTEAVQVLGGYGYV
                     REYPVERMMRDAKLTQIYEGTNEIQRVVIARTLA"
BASE COUNT          440 a          737 c          725 g          337 t
ORIGIN      
        1 atctgaccgg gcaggttctc ggtcaggtag ttcggtacgt cccccatctg gaagaaccac
       61 cagagcccgc cgagaatcag gaagacgcaa cctgtcgcgg cgacagggcc ggtgccgaac
      121 cgggcgccga tccggcttcc gatgaccgag aagatccccg ccatcgccgg acccgggaag
      181 aaggccagcc ctgccttcag aaccgagtag ccccagactc cggtcaggaa gagcacgccc
      241 ccgagcagga tcgcggcgaa ggcgaccatg aaaaccagcg cggccagact ggccattgcg
      301 aatggcctcg ccttgaacag gtccacgtcc agagtcggga tccagcgatc ggcgcgacgg
      361 cgggagcgca ggaaggtcag gccgagcatg gccacgcctc cgaggattgc gagcagggtc
      421 tgggcgctcc cccatccgtt gtcgggggct tcgaccaggc cccagcacag cacgccgatc
      481 ccgaggatca tcaggatcat ccccagcgga tcaggccagc gggtctcgtc ccggtctctc
      541 gattcaacca ggagcctcgt gccgaagtac caggctccga gggagagagg gaggttgacc
      601 aggaagaccc agcgccagga aatctcgacc aacagtccac cgatcggcgg tccgagcgca
      661 cccgcagcgc cgccggccgc ggcccagacg ccgatgacca cgggccagcg ctcggccggg
      721 aatgcgtgga gcagcaaggc cagcgatgtg gacatgagca ttgccgcgcc gactccctga
      781 accacccgga aggcgatcag ggtctcgacc gaccaggcgg cggcgcatgc cccggaggcg
      841 agacagaaga tcagaacccc ggtggtgaag acccgcttgc ggccgatccg gtcggcgagg
      901 cggccgaacg gcatcaacat cgcggcgaag acgatcacgt atccgttgag cacccaggat
      961 agagacccga tcgagctgcc cgggaagtcg gcctcgatcg ccggaaaggc gatgttgacg
     1021 atgaacatgt cgaggctggc gagggcaacc gccgccgcga ccacggccag tacccgaccg
     1081 aggatctcgg gtctgtaggc ctgttcggcc tcgatcatct cgaccggcac ggactcctcg
     1141 tccgcgaaag actcgattgc gaaatcctca gtaagttgcg tcatgcgact gaccctagca
     1201 gacatcggtc gcatgatgag actgacccgc tacaatcggt ggcatgctgg atcgtgagta
     1261 ctccaaccag gtctgttcga tcgcccgctc cctcgaggtc gtcggcgaga ggtggtcgct
     1321 gctgatcatc aggaacgtcg gcctgggcat gaaccgtttc gagccgctgc gcgaaaacct
     1381 cgggatcacc cgcagcgtgc tgaccacccg gctgaacacc ctgatcgaat acgggatcct
     1441 cgagaagcgt cagtacagcg agaagccgcc ccgctacgag taccacctca cccagaaggg
     1501 ccgggacctc tcccccgtcc tgctgcagtt gatgtggtgg ggcgacgagt actacccgga
     1561 acccggtgga ccgccgacca tcgcggtcca ccgcggaagc tgcggcggcg agctcgacca
     1621 tcacatggtc tgccagaagt gcggcgagtc agtcgaacct gaccgcgtct acatgaaacc
     1681 cggccccggc cggggtcagc aggacctacc caaaatcccc gcctgaccca ggtcgccggg
     1741 ccgtggggtc gggcaggtat ccccggaacc acggtgacgc aggtttcgga ccatcacggt
     1801 cgtaaacctg cggcacctcc ccaagaccta cgcgagagtg cgggcgatca ctacgcgctg
     1861 gatttcgttg gtgccttcgt agatctgggt gagcttggcg tcgcgcatca tccgctctac
     1921 cgggtactca cgcacgtagc cgtagccgcc gagcacctga accgcctcgg tggtgacctc
     1981 catcgcgaca tcggaggcga agagcttggc catcgccgag tacttgccca gcttggggtc
     2041 gttcgtctcg gccatcgagg cagccttgta gaggagctcc cgggcggcgg ccgtcttggt
     2101 ctccatgtca gcgagcttga actggatgcc ctgaagctgg ttgatcggct tgccgaacgc
     2161 aatccgctcc ttggcgtagt cggccgcgta gtcggtcgcg ccctgggagt cgaggttggt
     2221 gaggtactgg tcgaggaac
//
LOCUS       DAOTIP010000349         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57623_length_2236_cov_2.936268, whole genome shotgun sequence.
ACCESSION   DAOTIP010000349 DAOTIP010000000
VERSION     DAOTIP010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57623_length_2236_cov_2.936268"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1017)
                     /locus_tag="P5138_07130"
     CDS             complement(1..1017)
                     /locus_tag="P5138_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV60388.1"
                     /translation="MNTRFHVPTKVFGTRRSRGERPDGDSGSQSAPAMLSGTDPVLID
                     PMMNGSSGKTCSYLIQGTKTALVDPGSANAAPRVIEALKSRRIKVDLILLTHIHPDAA
                     AGAGVIAAKFPKARIAAPAGAAGKLADPVALVHDVKKVFGEKVEKIYGLPAAIDRSRI
                     TALGDGDRIDLGDRVIEAISTPGHTSAHMSFLDHSTASLFCGDALGVQLPGSRVVRPS
                     TPPWDFCLDDSLDSIERLADIDAETVFLAHFGAARPGPDEIFERAADSLESWHRSFLK
                     KLEQTDSEEDLSRQFNACLEATLEPIAPSVRRDLEMVNPAWLNLAGLSAEQARINGQF
                     SDAA"
     gene            complement(1265..1468)
                     /locus_tag="P5138_07135"
     CDS             complement(1265..1468)
                     /locus_tag="P5138_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRV60389.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHTAIQSEGFRTLAE
                     GAKVSYEAEDGPKGPAAASVEAI"
     gene            complement(1622..>2236)
                     /locus_tag="P5138_07140"
     CDS             complement(1622..>2236)
                     /locus_tag="P5138_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HRV60390.1"
                     /translation="HEAKVETLVSELESPNRGRTLVFARTKHGADRLVKKLSKRSRQV
                     RAVAMHGNKSQNQRQRALADFEDGRVDTLVATDVAARGIDVAEVTHVINFDIPEDQDT
                     FVHRVGRTGRAGADGVGISFFMSDQAKEMGKIARSLGLHSEFEASGAGFHAESHTRQK
                     KSDRRQPQNRGNDGGRQHGKPGKPKRNRSNASNRNRNSRRQRNR"
BASE COUNT          349 a          707 c          732 g          448 t
ORIGIN      
        1 tcaggcggcg tcggagaact gaccgttgat ccgcgcctgt tcggcgctca ggccggccag
       61 gttgagccag gccgggttga ccatttccag gtcgcgacgg acggacggtg cgatcggctc
      121 gagggtggct tcgaggcagg cgttgaactg ccgggagagg tcctcttcgc tgtcggtctg
      181 ctccagcttc ttgagaaacg agcgatgcca gctctccagg gaatccgcag ccctttcgaa
      241 aatttcgtcg ggcccgggcc gggcggcacc gaaatgcgcc agaaagacgg tttccgcatc
      301 aatgtcagcc agtctttcga ttgaatcgag cgagtcatcg aggcagaaat cccagggcgg
      361 ggtggagggg cggaccaccc ggctgcccgg cagctggacc ccgagtgcat ccccgcagaa
      421 cagggaggcg gtcgaatggt cgaggaagga catgtgcgcc gaagtgtggc ccggggtcga
      481 gatcgcctcg atcacccgat cacccagatc gatccggtca ccgtccccga gcgcggtgat
      541 cctcgaccgg tcgatcgcgg cgggaaggcc gtagatcttc tcgaccttct ctccgaagac
      601 ctttttcacg tcatgtacga gggctaccgg gtccgccagc ttcccggccg cgccggccgg
      661 ggccgcgatt ctcgccttcg gaaactttgc ggcgatcacc ccggcccccg cggcggcatc
      721 gggatggata tgggtgagca ggatcaggtc gaccttgatc cggcgggact tgagtgcctc
      781 gatcaccctc ggggcggcgt tggctgagcc cgggtcgacc agggcggtct tcgtcccctg
      841 aatcaggtag gagcaggtct tgccgctact gccgttcatc atcgggtcga tcaggaccgg
      901 atcggtaccg ctgagcatgg cgggggcgga ctggcttccg gagtcaccgt caggtcgctc
      961 gccgcggctg cggcgtgtgc caaagacctt ggtcgggacg tggaagcgcg tgttcatttc
     1021 accctttgag ttctctaacc gtccatcttc cagatggatt cggttttccc tctaaatccc
     1081 cgcaaatcct tctgttagtc gtcacaaagt gatcccgcgc cggcaactat gccggagggc
     1141 ccggacagca gcatgaaccc ggtgtgaagc gtgcgtgcga cgcaacccgg ttaaagcgaa
     1201 acgcccgccg gttggcgggc gctcgcggaa cttcggtcgg ggcacttgcc ccgggtgagt
     1261 cgacctagat ggcctccacc gaagcggcgg ccggaccctt gggtccgtcc tctgcttcgt
     1321 agctcacctt ggcgccttca gcgagggtgc gaaaaccctc ggactggatg gcggtgtgat
     1381 ggacgaaaac gtccttcgaa ccgtcgtccg gcgtgatgaa gccgaagccc ttttcgtcac
     1441 tgaaccactt gactgtgcca gtaggcattt agtcctccaa catcggtgct gcgaaacgcg
     1501 gaggatgccg gtaaagcaac gactgcgagc gctacacacg tctttgtcgg ggtttgctcc
     1561 ccccgaccca atggcgggac cgtagcagta tccgccagat ttcgcttgtt ttgttacaag
     1621 ctcagcggtt tcgctggcgg cgcgagttcc gattgcggtt cgaggcattg ctgcgattgc
     1681 gcttcggctt gcccggcttg ccgtgctgcc gcccgccgtc attgccgcgg ttctgcggct
     1741 ggcgccggtc ggacttcttc tgtctcgtgt ggctctcggc atggaagccc gcgccgctgg
     1801 cctcgaactc gctgtggagg cccagggacc gggcgatctt ccccatctcc ttcgcctggt
     1861 ccgacatgaa gaagctgatt ccgacaccgt cggccccggc gcgaccggtg cggccgaccc
     1921 ggtggacgaa tgtgtcctgg tcctcgggaa tgtcgaagtt gatcacgtgg gtgacctcgg
     1981 cgacgtcgat tccccgggcc gccacatcgg tggcaaccag ggtgtcgacc cgcccgtcct
     2041 cgaagtcggc gagggcccgc tggcgctggt tctgcgactt gttcccgtgc atcgcgaccg
     2101 cgcgtacctg cctgcttcgc ttgctcagct tcttgaccag ccggtcggcg ccatgcttgg
     2161 tgcgggcgaa aacgagggtt cggccccggt tcgggctttc cagttcactg accagggtct
     2221 cgaccttggc ttcatg
//
LOCUS       DAOTIP010000350         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57640_length_2235_cov_4.390367, whole genome shotgun sequence.
ACCESSION   DAOTIP010000350 DAOTIP010000000
VERSION     DAOTIP010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57640_length_2235_cov_4.390367"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="P5138_07145"
     CDS             complement(<1..198)
                     /locus_tag="P5138_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60391.1"
                     /translation="MRKTKAGHESWYGKWWSPEGRQVMKKIGPKRDRSGRGLDRSTAE
                     KKLREMIGDTPPPPESRVSVEE"
     gene            503..1495
                     /locus_tag="P5138_07150"
     CDS             503..1495
                     /locus_tag="P5138_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV60392.1"
                     /translation="MSNPFSSSQTLDVRYRSADQIRSGGALANLGNGPVIDSHGTPAR
                     LIAWPGTGFQTEAVHVLTLEPGHEGERYRYDMAEEAFLCHSGTAEVWLRGQWVSLEPG
                     DIAYVPEGAERAIRNTGKREAILVVQITPPQIDLYVDHGFYNEEWAVINHDASDKARA
                     TAATVEMPEAEFEFRETHPEMRAWNLEWEEVRRSGALFNVLMGTAFSGIGLPMRLILW
                     PGAGSRSVGFNYAYDPNGVTDELHTHPVSDECLIMWRGSGKFFIGGSWIDAKAGDVAL
                     APCGVAHGHTSVGDETWLGGFASPPQLDLLMPTDYYDKGSFTAPTPTRLEVDTD"
     gene            1560..2189
                     /locus_tag="P5138_07155"
     CDS             1560..2189
                     /locus_tag="P5138_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRV60393.1"
                     /translation="MEGLLQAGGVAVVALFPIIDPIGNTPWFIGATRDLDARARRSQA
                     FRGAWIASVILIIFLLAGSFLLDFFGVSLAAVEFVGGLVIGWVGWQMLMDAPSKDLGE
                     AAAPADRDVSFSPLAFPLLVGPGALAIALGLTNRHDSAADYVGFCLGIVVVMAISFVI
                     LTRAGELIARIGPRGIDVINGILGLIVLAIGAELVFHGISDHFGLTIVD"
BASE COUNT          383 a          714 c          699 g          439 t
ORIGIN      
        1 ttcctcaacc gagactcggc tctcgggtgg cgggggagtg tcgccgatca tttcccgcag
       61 tttcttctcg gcggtagatc ggtccaggcc gcgaccgctg cggtccctct tcgggccgat
      121 cttcttcatg acctggcgac cctccgggga ccaccacttg ccgtaccagg actcgtgacc
      181 ggccttggtc ttcctcacct ctaaatgccc tgctccgggt gggcgacgga cctttgagcg
      241 actcctggac ataggcccat tctaccgccg gatataccaa actcccgtga tctcagatca
      301 actcagacct ctgaacctgg ctcacagagc cgaaaacaag tgggcctctc tcgatcgggc
      361 tcgctcataa cccgaaggtc gcaggttcga atcctgcccc cgctatgttc gccagggtga
      421 ccgagtcacc cttcgcgaat agcttctatc caggccccgg agcgatttgt cgttgctcgg
      481 gcaatggtga ataatcggcg ccatgtcgaa tccgttctct tcgtcgcaga cgttggacgt
      541 ccgttaccgg tcggcagacc agatcagatc gggtggtgcc ctggccaatc tcgggaacgg
      601 tccggtgatc gattctcacg gcacgcccgc gaggctgatc gcctggcccg gcaccggctt
      661 tcagaccgaa gccgttcacg tgctcaccct cgaacccggc cacgaaggcg agcggtaccg
      721 ctacgacatg gccgaggagg ccttcctctg ccacagcggc acggccgagg tctggctgag
      781 gggccagtgg gtttcgctcg agccgggtga catcgcctac gtaccggagg gtgcagagcg
      841 ggcgatccgt aacaccggca agcgggaggc aatcctcgtc gtccagatca cgccgcccca
      901 gatagacctc tacgtcgacc acggtttcta caacgaggaa tgggctgtga tcaatcacga
      961 cgcgtccgac aaggcgcgtg ccaccgccgc cacggtcgag atgccggagg ccgaattcga
     1021 attcagggag acccacccgg agatgcgcgc ctggaacctg gagtgggagg aggtcaggcg
     1081 ctccggggcg ctgttcaacg tgctcatggg aacagcgttc agcggcatcg ggcttccgat
     1141 gcgcctcatc ctctggcccg gagccggctc gcgcagcgtg ggcttcaact atgcgtatga
     1201 ccccaatggg gtgaccgacg agctccatac ccatccggtc tcggatgagt gcctgatcat
     1261 gtggcgcggc agcggcaagt tcttcatcgg cggcagctgg atcgatgcga aagcgggcga
     1321 cgtcgcgctc gcgccatgcg gggtggccca cggccacacc agcgtgggcg acgagacctg
     1381 gctcggcggc tttgcctcac ccccgcagct ggaccttctc atgccgaccg actactacga
     1441 caaggggagc ttcaccgcac cgacacccac ccgcctcgag gtcgacaccg actgacgaac
     1501 cccgaccggc acgctggcaa tagcgggaat aacctcaggt agcgttagca tcgaacgtga
     1561 tggaggggct cctgcaggcg ggcggggtag cggtggtggc cctttttccg ataattgatc
     1621 cgatcggaaa tacaccctgg ttcatcgggg caaccagaga tctcgatgcg cgcgctcgcc
     1681 gctcgcaggc tttcaggggg gcgtggatcg cgagcgtcat cctcatcatc ttccttctcg
     1741 cggggagttt cctgctcgac ttcttcgggg tttcgctcgc tgcggttgag ttcgtcgggg
     1801 gcctggtaat cggctgggtc ggctggcaga tgctcatgga tgcgccgagc aaggatctcg
     1861 gcgaagcggc cgccccggcc gaccgtgacg tttccttcag tcctctggct ttcccgttgc
     1921 tggtcggtcc cggtgccctg gcgatcgccc tgggcctgac aaaccgccat gacagcgccg
     1981 ccgattacgt cggtttctgc ctggggatcg tcgttgtgat ggcgatcagt ttcgtgattt
     2041 tgaccagagc cggagagctg atcgccagaa ttggcccccg cgggatagac gtgatcaacg
     2101 gcatactcgg tttgatcgtt ctcgccatcg gggccgagct ggtcttccac ggaatctcag
     2161 accatttcgg cctgacgatc gtcgactgag gcgggctgta cgtcccgtct atcgaattga
     2221 atctggaggt cggag
//
LOCUS       DAOTIP010000351         2229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_57865_length_2229_cov_3.657314, whole genome shotgun sequence.
ACCESSION   DAOTIP010000351 DAOTIP010000000
VERSION     DAOTIP010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57865_length_2229_cov_3.657314"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="P5138_07160"
     CDS             complement(<1..485)
                     /locus_tag="P5138_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRV60394.1"
                     /translation="MSELLTLGLSHKTAPLELRERLALPEGRAAGLMNELTGNGDAAE
                     ATIVSTCNRTEVYLVAPDPVVAESEILSALAAQAGIRPTELVGHLYSLRGPDAARHLF
                     RVTAGLESMVLGEAEIQGQVKRAHELALVEGASGPILNRLFRGAIAAGGRVRNETGIS
                     E"
     gene            complement(478..714)
                     /locus_tag="P5138_07165"
     CDS             complement(478..714)
                     /locus_tag="P5138_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60395.1"
                     /translation="MSDVLPGDAGYVFAAYIVFLALLLIYLGILGAKFQRISRDLTEL
                     TGEVEAGRAPETAGSEGPAETGSAEAGAKEPTGV"
     gene            complement(715..1467)
                     /locus_tag="P5138_07170"
     CDS             complement(715..1467)
                     /locus_tag="P5138_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HRV60396.1"
                     /translation="MSGRGLGKLSILTALVLLAGMLLVFFWVPMDAVEGFRQKIFYIH
                     VPLAIVALCGFVAGGVEGFRHLRTNDRRHDANSYVFIHISVVFGVATLITGMLWAKAS
                     WGHYWVWDEPTLVSFLIVFLLYCTYYPFRYAIEDRDRQARYASVFAITAGAFVPLNFM
                     AVRMAQSLVHPRVFATSEGGLPGSMLFTFLVCLVGVGLLWVTLVKLELLGKQTSGQVS
                     KLRRALEAEGFGGNTEATGTVGRRIAPNLEAR"
     gene            complement(1491..2171)
                     /locus_tag="P5138_07175"
     CDS             complement(1491..2171)
                     /locus_tag="P5138_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRV60397.1"
                     /translation="MTPSRQAFTAIVAKDLRTELRTMRSLPAMILFAVTTFIIFRFGL
                     DRTALSGSLASGVIWTTLLFAAILGINRIFLPEREEGGFDAFRLAPVDRPVLFAAKAA
                     VLFLYLAVLEVIVVPVFAVFFLQDWGGLIPLVLVLLATNLGFAALGTLISSMAVNSRA
                     RDLLVPLLLLPLVVPLMIAATGATSPLLDAGGPSYDKFGTWLLVLGLYDGVFCLVGYA
                     VFDFLLED"
BASE COUNT          444 a          722 c          735 g          328 t
ORIGIN      
        1 ttctcgctga tgccggtctc gttccggacc ctgccgccgg ccgctatcgc gccgcggaag
       61 agccggttca ggatcgggcc ggacgcgcct tcgaccaggg caagctcatg ggcccgtttg
      121 acctggccct ggatctccgc ttcgcccaga accatcgatt caagcccggc cgtgacccgg
      181 aagaggtgcc gggccgcatc ggggccgcga agcgagtaga ggtggccgac cagttccgtc
      241 ggccggatgc ctgcctgtgc agcgagggcg ctcagaatct cggattccgc cacgaccggg
      301 tcgggagcga ccagatagac ctcggtccgg ttgcaggtcg agacgatcgt cgcctcggcc
      361 gcgtcgccgt tgccggtcag ctcgttcatc aggccggcgg cccggccctc cggcagggcg
      421 agtcgctctc gaagctccag cggagccgtc ttgtgggaaa gcccgagtgt gagtagttca
      481 gacaccggtt ggctctttgg ccccggcttc ggccgacccg gtttcggccg gtccttcgga
      541 tcccgccgtt tcgggcgcac ggcccgcctc gacctctccg gtcagttcgg tgagatcccg
      601 gctgatccgc tggaatttcg ctccgaggat tcccagatag atcagcagca gggccagaaa
      661 gacgatgtag gcggcgaaaa cgtatccggc gtcgccgggc agcacatcag acatctacct
      721 tgcctccagg ttcggtgcga ttcggcggcc gacggtcccg gtcgcctcgg tgttaccgcc
      781 gaaaccctct gcctcgaggg cgcgcctcag cttcgagacc tggccggagg tctgcttgcc
      841 gagcaactcc agcttgacca gggtcaccca cagcagtccg acgccgacca ggcagaccag
      901 gaaggtaaac agcatcgacc cgggcaggcc gccctcgctg gtcgcgaaga cccgcggatg
      961 gaccaggctc tgcgccatcc gcaccgccat gaagttgagc ggcacgaaag caccggcggt
     1021 gatcgcgaag accgacgcgt aacgggcctg cctgtcccgg tcctcgatcg cgtagcggaa
     1081 cgggtaatag gtgcagtaga gaaggaagac gatcaggaag ctgaccaggg tcggctcgtc
     1141 ccagacccag tagtggcccc aggaagcctt ggcccagagc atgccggtga tcagggtggc
     1201 caccccgaag acgaccgaga tgtggatgaa gacatatgag ttggcgtcat ggcgccggtc
     1261 gtttgtccgc aggtgacgga aaccctcgac accgccggcg acgaatccgc agagcgcgac
     1321 aatcgcgagc ggcacgtgga tgtagaagat cttctggcgg aacccttcga ccgcatccat
     1381 cgggacccag aagaagacca gcagcatgcc ggccagcagc accagcgccg tgagaatgga
     1441 gagttttcct agaccgcgtc ccgacatttc ggattccgcc cggtgccgac tcaatcctca
     1501 agcaggaagt cgaaaacggc ataaccgacg aggcagaaga ccccatcgta tagaccgagg
     1561 accagaagcc atgtcccgaa cttgtcgtag gaaggcccgc ctgcatccag cagcggtgaa
     1621 gttgccccgg tggcggcgat catcagcggc accacgagcg gcagcaacag cagcggcacc
     1681 agcaggtcgc gggcccgcga gttgaccgcc atcgacgaga tcagcgtgcc aagggccgcg
     1741 aatccgagat tggtcgccag caggacgagg accaggggga tcagtccgcc ccagtcctgg
     1801 aggaagaaga ctgcgaaaac cggcacgaca atgacctcga gcacggccag atagaggaag
     1861 agcaccgcgg ccttggcggc aaagagcacc ggccggtcaa ccggcgcgag ccggaaagca
     1921 tcgaagccgc cttcctcccg ttccggcagg aagatccggt tgatcccgag aatcgccgcg
     1981 aagagcaggg tggtccagat cactcccgag gcgaggctgc cggaaagggc cgtgcggtcg
     2041 agaccgaagc ggaagatgat gaaggtggtc accgcgaaga ggatcatcgc cggcagagag
     2101 cgcatcgtgc gcaactcggt acgcaggtcc ttggcgacga tcgccgtgaa agcctggcgg
     2161 gacggggtca cgcgaccacc ccgccgatcg ccagttcgag tacccggtcg gattcttccc
     2221 gcgccttct
//
LOCUS       DAOTIP010000352         2224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_58040_length_2224_cov_3.467958, whole genome shotgun sequence.
ACCESSION   DAOTIP010000352 DAOTIP010000000
VERSION     DAOTIP010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58040_length_2224_cov_3.467958"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="P5138_07180"
     CDS             <1..1360
                     /locus_tag="P5138_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV60398.1"
                     /translation="SPLKDDLASESIAAVTAGVTTWGVHAPSTRMGHPDFAEYVQKED
                     VMSFHDSFPYFEALTESDSAIDIFLTYMLETDQQAAEIPEYAKDHGVTSYKLYLQAMS
                     PEAEPNWPGRRAGLGAGFDDGVCWVTMENVAALGRPGVVCMHCENWEIARIFDKRLKE
                     EGRTDWATWSDRSPHYLEASHIRWYGDMAAEIGCPIYIQHSTTPESYKEILELRGRGV
                     EIYAQTGPHWLHFGKGEHNAWRINVPLRSRENNPNIWTALQCGIIDSVGSDHVVAWEP
                     ADYESSYNENIWELKTGFTSRVEMHLPVLLEGVHQGKLTIERLVEVACANPARIFGVY
                     PQKGLIEVGADADIVLVDLNKEVTVKNENVLTRSGWTCIDGHTIHGYPVATFLRGKQM
                     SKFDDGAPGPEFIGDADGHYIRRTPGQDLLPVAGGEGARAEVRKLPKLGKPEGSAVGI
                     DV"
     gene            1353..2129
                     /locus_tag="P5138_07185"
     CDS             1353..2129
                     /locus_tag="P5138_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRV60399.1"
                     /translation="MSETGDSGNVWDDVLPEEDRLVFEAAGWGKDVGFGEKPVLLVVD
                     VIYNFCGDVPEPILESIKRWRYSCGERSWEGVRHLERLIAAAREKQIPIVYTGMDRRP
                     DGFDQGAWNWKSYRSGEASDIKGSLGNEIVEEIKPEPQDIYFVKDKPSAFHGTHLLDY
                     LIYLGADTVITTGTTTSGCVRASAVDATQYNYRSIVPEECVWDRSMISHKVNLLDIQM
                     KYGDVKKTDQVIEYFKSLPDRTCGPNTPTGVANTDQEVPK"
BASE COUNT          439 a          742 c          713 g          330 t
ORIGIN      
        1 ctcgccactc aaggacgacc tcgccagcga atcgatcgct gcggtcaccg ccggcgtcac
       61 cacctggggg gtccacgccc cctcgacccg gatgggccat cccgacttcg ccgagtacgt
      121 ccagaaggaa gacgtcatgt ccttccatga ctccttcccc tacttcgagg cgctgaccga
      181 atccgactcg gcgatcgaca tcttcctcac ctacatgctg gagaccgacc agcaggccgc
      241 ggaaatcccc gagtacgcaa aggatcacgg cgtcacctcc tacaagctct acctccaggc
      301 gatgagcccc gaggccgagc cgaactggcc gggccgccgg gccggactcg gtgccggctt
      361 cgacgacggc gtctgctggg tgaccatgga gaacgtcgcg gcgctcggcc gtcccggcgt
      421 ggtctgcatg cactgcgaga actgggagat cgcccgcatc ttcgacaagc gactgaagga
      481 agaggggcgg accgactggg cgacctggtc ggaccgctcc ccccattacc tcgaggcctc
      541 gcacatccgt tggtacgggg acatggccgc cgagatcggc tgcccgatct acatccaaca
      601 ttcgaccacc ccagagtcct acaaggagat cctcgaactg cgcggccgcg gggtcgagat
      661 ctacgcccag accggtccgc actggctgca cttcggcaag ggcgagcaca acgcctggcg
      721 gatcaacgtg ccgctccgct cccgcgagaa caatccgaac atctggacag cgctgcagtg
      781 cgggatcatc gattcggtcg gctccgacca cgtggtcgcc tgggagcccg ccgattacga
      841 gtcctcctac aacgagaaca tctgggagct caagaccggc ttcacctcca gggtcgagat
      901 gcacctgccg gtcctgctcg aaggggtcca tcagggcaag ctcacgatcg agcgcctggt
      961 cgaggtggcc tgcgcgaacc cggcccggat cttcggcgtc tacccgcaga agggactgat
     1021 cgaggtcggc gccgacgccg acatcgtgct ggtggacctg aacaaggaag tcacggtcaa
     1081 gaacgagaac gtgctgaccc gctcgggctg gacatgcatc gacggccaca ccattcacgg
     1141 ctacccggtc gccaccttcc tgcgcggcaa gcagatgtcg aagttcgacg acggtgcccc
     1201 cggcccggag ttcatcggtg atgccgacgg tcactacatc cgccggaccc cgggccagga
     1261 cctgctgccg gtggccggcg gcgaaggggc ccgggccgag gtgcggaagc tgccgaagct
     1321 gggcaagccc gaaggcagcg cagtggggat cgatgtctga gacgggcgac agcggcaacg
     1381 tctgggacga cgtcctgccg gaagaagacc ggctggtctt cgaggccgcc ggttggggca
     1441 aggacgtcgg cttcggcgag aagcccgtcc tgctggtcgt cgacgtgatc tacaacttct
     1501 gcggcgacgt gccggagccg atcctcgaat cgatcaagcg ctggcgctac tcctgtggcg
     1561 agcgcagctg ggagggtgtc aggcacctcg agcggctgat cgccgccgcc cgcgagaagc
     1621 agatcccgat cgtctacacc ggcatggacc gccggccgga cggcttcgac cagggtgcct
     1681 ggaactggaa gagctatcgc tccggcgagg cctctgacat caagggctcg ctcggaaacg
     1741 agatcgtcga ggagatcaag ccggagccac aggacatcta cttcgtcaag gacaagccaa
     1801 gcgcctttca cgggacccac ctgctcgact acctgatcta tctgggggcc gacacggtga
     1861 tcacgaccgg caccacgacc agcggctgcg tgcgggccag cgcggtcgat gccacccagt
     1921 acaactaccg ctcgatcgtg ccagaggaat gcgtctggga ccgctcaatg atctcccaca
     1981 aggtcaacct gctcgacatc cagatgaaat acggggatgt gaagaagacc gaccaggtga
     2041 tcgaatattt caagtccctg ccggaccgga cctgcgggcc gaacacccct accggcgtcg
     2101 ccaacaccga ccaggaggtg ccgaagtgag tgaagagctg gacctcgacg tggaagtccg
     2161 gaagatcgtc accgtggtcg aagaagtcca caccgagggc ggccggggtg acggcggcgg
     2221 cccg
//
LOCUS       DAOTIP010000353         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_58119_length_2222_cov_2.469312, whole genome shotgun sequence.
ACCESSION   DAOTIP010000353 DAOTIP010000000
VERSION     DAOTIP010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58119_length_2222_cov_2.469312"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="P5138_07190"
     CDS             <1..391
                     /locus_tag="P5138_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV60400.1"
                     /translation="VATTVIEVGIDVPNATVMVIEGAERFGLSQLHQLRGRIGRGSHG
                     GTCFLFSESGGARAARRLAALASTTDGFRLAELDLEMRGEGEITGTRQHGLPRFRVAS
                     LPRDVDLLEAARVDLERLSEQDLELLS"
     gene            388..948
                     /locus_tag="P5138_07195"
     CDS             388..948
                     /locus_tag="P5138_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRV60401.1"
                     /translation="MRVIAGSLGGRQLLAPKGWTVRPTSERVREAIFSALGDISGMRV
                     ADLYCGTGALGIESISRGAASAVLVDRDTRPAMGNVHNLGITGQAELVRAEAPDWIRS
                     AGSASFDLVFLDPPYREARAVAERLDPELERVLAPGGRVIAESDRRGPLEFPSLETVR
                     ERRYGRTVVTFHRVPSKTPQDPNQPE"
     gene            945..1448
                     /gene="coaD"
                     /locus_tag="P5138_07200"
     CDS             945..1448
                     /gene="coaD"
                     /locus_tag="P5138_07200"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRV60402.1"
                     /translation="MNDTTEKVAVCPGSYDPVTNGHLDIITRASRTFDRVVVGVVNQP
                     IRKKKTLFTADERIAFIENATAELGNVEVRAFYTLLVEFARDMGANAIVKGLRAISDF
                     EYENEMAQLNYKLDPGVESIYVFARPEYSFLSSTGVKEMATFNGDIGDLVPAEVAAAL
                     ADRLGRS"
     gene            1552..2025
                     /locus_tag="P5138_07205"
     CDS             1552..2025
                     /locus_tag="P5138_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRV60403.1"
                     /translation="MDVLVLIDKLDDLVHNARPVPLTDQVRVDREEIYDLLDQMRATI
                     PEEIKQARWIVKERQEMLEEAKRESERVVREAREQQARLVSDEEVTKQAERAADEIVE
                     DARAREREIRLGAEDYADEILNTLEVNLQKFTAAVQRGRDRLAGRDDEPLDEIEE"
     gene            complement(2035..>2222)
                     /locus_tag="P5138_07210"
     CDS             complement(2035..>2222)
                     /locus_tag="P5138_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60404.1"
                     /translation="RVREQSRALVIGDFVALGEDRGETQVPGTAYPDTAEELAGWCGG
                     EVVWQDDDLVVIVADYR"
BASE COUNT          421 a          716 c          722 g          363 t
ORIGIN      
        1 cgtggccacg acggtgatcg aggtggggat cgacgtgccg aacgcgaccg taatggtgat
       61 cgagggggcg gagcgattcg gcctctctca gcttcatcag ctccgcggcc ggatcggccg
      121 ggggagccac ggcggaacct gtttcctgtt cagcgagagc ggtggcgccc gcgcggcccg
      181 tcgtctcgcg gctctcgcct cgaccacaga cggtttccgg ttggccgagc tcgacctcga
      241 aatgaggggg gagggggaga tcaccgggac gaggcagcac ggcctgccgc ggttccgggt
      301 cgcctcgctg ccccgggacg tcgacctgct ggaagcggca agggtcgacc tcgaaaggtt
      361 gagcgaacag gatctggagc tgctctcatg agggtgatcg ccggaagcct tggcggccgc
      421 cagttgctcg cgccgaaggg ctggaccgtg cgcccgacct ctgaacgggt ccgggaggcg
      481 atcttctccg cgctcggaga catcagcgga atgcgggttg ccgatctcta ctgcggtacg
      541 ggtgcgctcg ggatcgagtc aatctcccgg ggtgccgctt cggcggtgct ggtcgaccgg
      601 gacaccaggc ccgccatggg caacgtccac aacctcggga tcaccggcca ggcggaactc
      661 gtccgggccg aggctcccga ctggatcagg tccgccggga gcgcgtcatt cgatctggtg
      721 tttctcgatc cgccctaccg ggaggcgagg gccgttgcgg agcggctcga cccggaactg
      781 gagcgggtgc tcgcccccgg aggccgggtg atcgcggaat cggatcgccg cggtccactg
      841 gaatttccct ccctcgagac cgtgcgggag cgacgttacg gtcgcacggt ggttacattc
      901 catcgagttc cgtccaaaac ccctcaagac ccgaaccaac ctgaatgaac gacacaaccg
      961 agaaagtcgc cgtctgtccg ggcagctacg acccggtaac caacggtcac ctcgacatca
     1021 tcacccgcgc ctcccgcacc ttcgacaggg tggtggtcgg ggtggttaac cagccgatcc
     1081 gcaagaagaa gacacttttc acggcggacg agcgaatcgc cttcatcgag aatgcaaccg
     1141 cggaactcgg caatgtcgag gtcagggcct tctacacgct gctggtcgaa ttcgcccgcg
     1201 acatgggagc caacgcgatc gtcaagggcc tgcgcgcgat ttccgacttc gaatatgaaa
     1261 acgagatggc ccagctcaac tacaagctcg atcccggggt cgaaagcatc tacgttttcg
     1321 cccggcccga atacagcttc ctttcgtcca ccggcgtcaa ggagatggct accttcaacg
     1381 gcgacatcgg tgatctggtc ccggccgagg tggcagccgc actggcggat agactggggc
     1441 gcagttagtg tcgtgaagac acaaagagga atggaagatt ccatgcagaa tcttcaccac
     1501 atggccacat tttccccgca aatcgacgga gacgcatagg gttccctcca catggatgtc
     1561 ctcgttctaa tagacaagct cgatgacctc gtccacaacg cccgtccggt ccccctgacg
     1621 gaccaggttc gcgtggaccg tgaggagatc tacgacctgc tcgaccagat gcgggccacg
     1681 atccccgagg agatcaagca ggcacgctgg atcgtcaagg agcgtcagga gatgctcgaa
     1741 gaggcgaagc gcgaatccga gcgggttgtc cgcgaggcgc gcgagcagca ggcccgcctg
     1801 gtttccgatg aggaagtcac caagcaggcc gagcgtgctg ccgatgaaat cgtcgaagac
     1861 gccagggccc gcgagcgtga gatccgcctc ggcgccgagg attacgccga cgagatcctc
     1921 aacaccctcg aggtcaacct ccagaagttc accgccgcag ttcagcgggg ccgcgaccgc
     1981 ctcgccggcc gcgacgacga gccgctcgac gaaatcgagg agtaggaagc tccgctatcg
     2041 gtagtcggcg acgatgacca cgaggtcgtc gtcctgccag accacttcgc ctccgcacca
     2101 gccggctagc tcttcggccg tgtccggata ggcggttccg gggacctggg tttctccgcg
     2161 gtcctcgccg agcgcgacga agtcgccgat caccagtgcc ctcgactgtt cccggacccg
     2221 cc
//
LOCUS       DAOTIP010000354         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_58468_length_2212_cov_2.817803, whole genome shotgun sequence.
ACCESSION   DAOTIP010000354 DAOTIP010000000
VERSION     DAOTIP010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58468_length_2212_cov_2.817803"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="P5138_07215"
     CDS             <1..187
                     /locus_tag="P5138_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRV60405.1"
                     /translation="QLRNCLFAREELDLKGALRSGASDEGIRDILRAGVAAKRAGHGI
                     DSPDFTQPDRGMNAIGG"
     gene            complement(206..442)
                     /locus_tag="P5138_07220"
     CDS             complement(206..442)
                     /locus_tag="P5138_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRV60406.1"
                     /translation="MSVRFFAGAAEAFGATETQVEGADTVAGLIESLAGADERLRMVL
                     DQCSFFVDGEHSTALEKPLPEDGRIDVLPPFAGG"
     gene            complement(448..726)
                     /locus_tag="P5138_07225"
     CDS             complement(448..726)
                     /locus_tag="P5138_07225"
                     /inference="COORDINATES: protein motif:HMM:NF040429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HRV60407.1"
                     /translation="MNQENPKHLPPEALDNWLEKVSGLLGIEEEIDVGAVLDLTRDVA
                     HDVARPAAPLTTFALGLALGRLPADQFEPKFAELQAALTRLAAEQQEA"
     gene            complement(723..1364)
                     /locus_tag="P5138_07230"
     CDS             complement(723..1364)
                     /locus_tag="P5138_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRV60408.1"
                     /translation="MGSPRREDRRQIRHLAEAGLIPEPATAVVLAGGRGSRLGGAEKG
                     EIRLDGRRLIDIVVAAARESGCERIVIAGDIESEGAITIREDPPFGGPAAGLAAALPA
                     IEDDWILLLACDLPHARLLIDLLTGAFREVGPEVDGLIALRNNHPQWLAGFYRRRSIE
                     DALGRAGDPNDISMKSLFRGLKLREVQDPEGLSRDLDTPEDLEEFAKKGEVEK"
     gene            complement(1304..1774)
                     /gene="moaC"
                     /locus_tag="P5138_07235"
     CDS             complement(1304..1774)
                     /gene="moaC"
                     /locus_tag="P5138_07235"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRV60409.1"
                     /translation="MTFTHLDESGQARMVDVTAKQPTVRSATASGFVRCPPAVIAALR
                     DEDLPKGDALAVARVAGIQGAKKCAELLPLAHVIGVHGAAVSFETGDEGVSVSATVRT
                     ADRTGVEMEALTAVSVAALTLIDMFKGIDKGVTIESVRLEEKTGGKSGTWRRQA"
     gene            complement(1771..>2212)
                     /locus_tag="P5138_07240"
     CDS             complement(1771..>2212)
                     /locus_tag="P5138_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60410.1"
                     /translation="DRVAMQPGKPQAFGRLGESGPLVFGLPGNPVSAWVSFQMFVRPV
                     LRKMQGAARLFPEAVPARATEDWKTPQGRMQVIPVRISDENERKVLPAATGGSGSHLI
                     ASLAAADGYALVEPEVEKIKAGDLVPTVRLRDPEQFNHPQRRQP"
BASE COUNT          345 a          774 c          725 g          368 t
ORIGIN      
        1 tcagctgcgc aactgcctct tcgcccgcga ggagctcgac ctgaaaggag ccctgcgcag
       61 cggtgcttcc gacgagggga tccgcgacat cctgcgggcc ggggttgccg ccaagcgggc
      121 cggccacggc atcgacagtc cggacttcac ccagccggat cgaggcatga acgcgatcgg
      181 cggctgaagc cgcccggttg cccgctcagc cgccggcgaa gggcggcagc acgtcgattc
      241 ggccgtcttc cggaagcggc ttctcgagcg cggtgctgtg ttcaccgtcg acgaagaacg
      301 agcactggtc gaggaccatc cgcaaccgtt cgtcggcccc ggccagtgac tcgatcaacc
      361 cggccacggt gtcggcccct tcgacctgcg tttcggtagc accgaaagct tcggccgccc
      421 cggcaaagaa cctgaccgac acccgggtca agcttcctgc tgttccgccg cgagccgggt
      481 cagggcagcc tgcagctcgg cgaatttcgg ctcgaactgg tcggccggca gccggcccag
      541 cgccaggccg agcgcgaagg tggtgagcgg cgcggcgggc cgggccacgt catgggctac
      601 atccctggtc aggtcgagca cggccccgac gtcaatttcc tcctcgatcc cgagcagccc
      661 ggaaaccttc tccaaccagt tgtcgagggc ctccggcggc agatgcttcg gattctcctg
      721 gttcatttct ccacctcgcc tttctttgcg aactcttcaa gatcttccgg ggtgtcaagg
      781 tcccgggaca atccttccgg atcctggacc tctcgtagct tcagacctcg gaaaagggat
      841 ttcatcgaaa tatcgttcgg gtcacccgcc cggcccaacg cgtcttcgat cgagcggcgt
      901 cgataaaagc cggccagcca ctgaggatgg ttgttccgca gcgcgatgag cccgtcgact
      961 tccgggccga cctcgcggaa ggcgccggtc agcaggtcga tcagaagccg ggcatgaggg
     1021 aggtcgcagg ccagcaggag gatccagtcg tcctcgattg ccggcaaggc agccgcgagc
     1081 ccggccgccg gcccgccgaa gggcggatcc tcacggatcg tgatcgcccc ttcggattcg
     1141 atgtcgccgg cgatgacgat cctttcgcag ccgctttcgc gggcggcagc cacgacgatg
     1201 tcgatcaggc gccggccgtc cagtctgatt tcgcccttct cggcgccgcc gagccgggag
     1261 ccccggccgc cggcgaggac gaccgcagtg gcgggttcgg gaatcaggcc tgcctccgcc
     1321 aggtgccgga tttgccgccg gtcttctctt cgaggcgaac cgattcgatc gtcacgccct
     1381 tgtcgatccc cttgaacatg tcgatcaggg tgagggcggc aaccgagacc gcggtcaggg
     1441 cctccatctc gactccggtc cggtcggcgg tgcgcaccgt cgcgctgacg ctcacccctt
     1501 cgtccccggt ctcgaatgac accgcggcac cgtgcacgcc gatcacgtgg gccagcggca
     1561 ggagctcggc gcatttcttg gccccctgga taccggccac ccgggccacg gccagcgcat
     1621 cacccttcgg caagtcctca tcgcgcaggg ccgcaatcac cgctggcggg cagcgcacga
     1681 aacctgaagc cgtggccgac cgcacggtag gctgcttggc ggtcacgtcg accatccggg
     1741 cctggccgct ttcgtctagg tgggtgaagg tcaaggttgt ctcctctggg ggtggttgaa
     1801 ctgctccggg tcacgcaggc ggacggtcgg tacgagatcg ccggccttga tcttctcgac
     1861 ctcgggctcg accagggcgt aaccgtcggc cgccgccagg ctggcgatca ggtgggagcc
     1921 ggagccaccg gtcgcagccg gcagcacctt tcgctcgttc tcgtcggaga tccgcaccgg
     1981 aatcacctgc atccggcctt gcggggtctt ccagtcctcc gttgcccggg ccgggaccgc
     2041 ctcgggaaag agccgggccg ccccctgcat ctttcgcagg acgggacgga cgaacatctg
     2101 aaaggaaacc caggcgctga ccgggttgcc gggcagcccg aagacgagcg gtccgctttc
     2161 acccagtcgg ccgaaggcct gcggtttgcc gggctgcatc gcaacccggt cg
//
LOCUS       DAOTIP010000355         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_58527_length_2210_cov_3.888631, whole genome shotgun sequence.
ACCESSION   DAOTIP010000355 DAOTIP010000000
VERSION     DAOTIP010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58527_length_2210_cov_3.888631"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="P5138_07245"
     CDS             complement(<1..1112)
                     /locus_tag="P5138_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRV60411.1"
                     /translation="MNRVKTFLLVLFASMLVAVVPAANAGTAPAGSSAVKKGKGKKKT
                     KKTKKVPAGAFKGALAPLSGKGRWFVDRTGRVVQLRGVNEVSKHAPYYPAADGFGADD
                     AALLARHGFNVVRLGVDFQGLMPEPGQPSETYISNLAKTVNVLTRAGIYTLVDFHQDG
                     FSPKYNGNGFPDWWAVDDGLSNPNVYFPVYYLLNPAMQRAFEHFWMNTTVEGKGLQDW
                     FIQGLLAVVKKFRSNSHVIGYETFNEPWPGADYSACYDAETGCPDLERERIMPFARKV
                     VAATRKLTKRQQIFVEPFVTYNFGTPTSLPGSTGASLAVHNYAVSAEGDAKVVEQSVK
                     AARRDSKPVVLTEFGATGDTAKLDRLTSLYDGGLMS"
     gene            complement(1237..1974)
                     /locus_tag="P5138_07250"
     CDS             complement(1237..1974)
                     /locus_tag="P5138_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRV60412.1"
                     /translation="MDLLVVSFLAGVLTAAAPCVLPLLPVIVGGSVLSGDEDEDRRSL
                     RHPLTIVISLAVSIIVFSLLLKTTTALLGIPDDVWSILAGGVVVLFGITLLWPSIWDR
                     LMVATGISAGANNLLDRSRDETGIKRDILLGAALGPVFNSCSPTYALIIAVTFPASFA
                     EGFINLIAYAAGLALILLLISLFGRAVVDRLNWLSDPHGTFRKVVGALFLVVGFGVIF
                     GLDRDLQAWILENGWYDPVQDVEGSLN"
     gene            complement(2004..>2210)
                     /locus_tag="P5138_07255"
     CDS             complement(2004..>2210)
                     /locus_tag="P5138_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60413.1"
                     /translation="EDDIEASGVPDGVTIFKVDYDSNQDLRQKYGVTLQTTMVKVDDN
                     GEKIDSYVAYEEPTLASVTAALLQ"
BASE COUNT          416 a          688 c          705 g          401 t
ORIGIN      
        1 caggacatga ggccaccgtc gtaaagcgag gtcagccggt ccagcttcgc ggtgtcaccg
       61 gtcgctccga actcggtcag caccaccggt ttgctgtcgc gtcgggcagc ctttaccgac
      121 tgttccacga ccttggcgtc accctcggcg ctcaccgcgt agttatgcac cgcgagtgag
      181 gctccggtcg agccgggcag tgaagtcggt gtgccgaagt tgtaggtgac gaacggttcc
      241 acaaagatct gctggcgttt ggtcagcttc ctggtcgccg cgacaacctt gcgggcgaaa
      301 ggcatgatgc gttcccgctc gaggtccggg cagccggtct ccgcgtcgta acaggccgag
      361 taatcggctc ccggccacgg ctcgttgaag gtctcgtagc cgatcacatg cgagttgctt
      421 cggaatttct tcactaccgc caggaggccc tggatgaacc agtcctggag gcccttgcct
      481 tcgaccgtcg tgttcatcca gaagtgctcg aatgcccgct gcatggccgg gttgaggagg
      541 tagtagaccg ggaagtacac gttcgggttc gagaggccgt cgtcgaccgc ccaccagtcg
      601 gggaaaccgt tgccgttgta cttcggggag aagccgtcct gatggaagtc gacgagtgtg
      661 tagatcccgg ccctggtcag aacgttcacg gtcttcgcca ggttcgagat gtaagtctcg
      721 ctgggctggc cgggctccgg catcagtccc tggaaatcca cgccgagccg caccacgttg
      781 aacccgtgcc tggccaatag agccgcgtca tcggctccga agccatcggc cgccgggtag
      841 tacggcgcat gcttcgaaac ctcgttgacc ccacgcagct gcaccacacg accggtgcgg
      901 tcgacgaacc agcggccctt gcccgaaagc ggggcgagcg cacctttgaa cgccccggcc
      961 ggaaccttct tggtcttctt ggtcttcttc ttgcccttcc ccttcttgac tgctgacgat
     1021 ccagccggtg cggtgccggc attggcagcc gggaccacgg caaccagcat ggatgcgaaa
     1081 aggaccagca ggaatgtctt gactcggttc atggattcca ttcggcacaa acgcgggaag
     1141 gcgcggctcg ccccccggct agtcaagaac aggttagacc agcattcgat gcgctattcg
     1201 aagggccgag tccgacaacc gcctgggccg gcccgtctag ttgagcgagc cttcgacatc
     1261 ctggaccggg tcgtaccacc cgttctcgag gatccatgcc tggaggtcac ggtcgaggcc
     1321 gaagatcacg ccgaatccga ccaccaggaa aagggctccg actaccttgc ggaaggtgcc
     1381 gtgtggatcg ctgagccagt tgagccggtc caccaccgcc cggccgaaca gactgatcag
     1441 cagcaggatc agggccagcc ccgcagcgta ggcgatcagg ttgatgaagc cttcagcgaa
     1501 ggaggcgggg aaggtaacgg cgatgatcag ggcataggtg gggctgcagc tgttgaatac
     1561 cggcccgaga gcggcaccga gcaggatgtc ccgtttgatc ccggtttcgt cccgtgacct
     1621 gtcgagcagg ttgttcgcac cggcggagat tccggtcgcg accatgagcc ggtcccagat
     1681 cgaaggccag agcagcgtga tgccgaacag caccacgacg ccaccggcga ggatcgacca
     1741 gacatcgtcg ggaatcccca gcagcgccgt ggtcgttttc agcagaagtg agaagacgat
     1801 gatcgagacg gccaggctga tcacgatcgt cagggggtgc cgaagcgatc gccggtcttc
     1861 atcttcgtcc ccgctcagca ccgatccccc gacgatcacc gggagaagcg gcaggacgca
     1921 gggcgcagcg gcggtcagta cgccggcgag gaaggagaca accagaaggt ccatcgatca
     1981 ggtcttgcgg accaaccggc cggttactgg agcagggccg cggtcacgct ggcgagggtc
     2041 ggctcctcgt aggcgacgta gctgtcgatc ttctcgccgt tgtcgtcaac cttgaccatt
     2101 gtcgtctgaa gggtgacccc gtacttctgg cggagatcct gattcgagtc gtagtcgacc
     2161 ttgaagatcg tcaccccgtc cgggacgcca ctggcctcga tgtcatcctc
//
LOCUS       DAOTIP010000356         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_58548_length_2210_cov_2.781903, whole genome shotgun sequence.
ACCESSION   DAOTIP010000356 DAOTIP010000000
VERSION     DAOTIP010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58548_length_2210_cov_2.781903"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="pyrF"
                     /locus_tag="P5138_07260"
     CDS             <1..789
                     /gene="pyrF"
                     /locus_tag="P5138_07260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRV60414.1"
                     /translation="DSITDWVDGSPAEAAGAAVSAHCRRIIDLAGPSCIAVKPQLACF
                     ERLGWPGWRALEEVCEAAREAGLLVVADGKRGDVPVSAKAYGQAMVGVTDSPFGPVDG
                     LGADAFTANPLLGEDALEPMVAAAEAAGAGVFSLVRTSNPGAADVEDLPTQDGPVHEQ
                     LARMVDRFGDRLGGRESRLSGMGAVVGATAPEHIARLRELMPRAMFLIPGVGAQGGRP
                     EDLGAAFVAGRAGAMVAASRSIAGADDPAAAAEELRAAVWAVSG"
     gene            896..1216
                     /locus_tag="P5138_07265"
     CDS             896..1216
                     /locus_tag="P5138_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRV60415.1"
                     /translation="MEEKKKNSQIARLLAAIALVGAVLILIVVISGNSGSDDKGNTKK
                     ANPAKQQAKKKPKTTAKTYEVKEGDTLTTIAHKTGVSVQRIEALNPDLDPQALQVGQE
                     LKLR"
     gene            1213..>2210
                     /locus_tag="P5138_07270"
     CDS             1213..>2210
                     /locus_tag="P5138_07270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRV60416.1"
                     /translation="MTGRPRLALLLLLLAVWGAGTATATAASAPERGPKLDAKAWIVI
                     DARTGQPLAGHAVNRHLGMASTTKMMTAYLSVRNLPFRKRVKAGKYDPDPAESLMGLE
                     PGQVVSVRDLLYGLMMLSGNDAAVTLAKAVSGSEKKFVRLMNRTARQLGLDDTHYDNP
                     VGLDGPSHYTSAADLAKLGRIVMGMPRLRKIAGSRKAKLTSYRPPVTIETTDSFLRDN
                     GWARGIKTGHTLGTGYTLASDGRRKATELIGAVIGTPTEEARNDETVRLLDWGFDQYT
                     KRVPVKVDKPVAMVPVRYEDQDLPVKSRRRVRIGVRKGESLTVVTDLPGEVEGPIPA"
BASE COUNT          399 a          706 c          771 g          334 t
ORIGIN      
        1 gactcgatca ccgattgggt cgacgggtcc ccggccgaag ccgccggggc cgccgtttcc
       61 gcccattgcc ggaggatcat cgatctggcc ggcccctcct gcattgccgt caaaccgcag
      121 ctcgcctgtt tcgagcgact gggctggcca ggctggaggg cgcttgaaga agtctgcgag
      181 gcggcccggg aagcggggct gctggtcgtc gccgacggca agcgcggcga cgttccggtt
      241 tcggcaaagg cctacgggca ggccatggtc ggggttacgg actcaccctt cggcccggtc
      301 gatggtctcg gcgccgacgc gttcaccgcg aaccccctgc tgggggaaga cgcattggag
      361 ccgatggttg ccgcggcgga agcggcgggt gccggtgtct tctccctggt ccggaccagc
      421 aaccccggcg cggcagacgt cgaggatctt cccactcagg atggaccggt ccatgaacaa
      481 ctggcgcgca tggtcgaccg cttcggtgat cgcctcggtg ggagggaaag cagactttcc
      541 ggcatgggag cggtggtcgg ggcgaccgcg cccgagcaca tcgcacgcct gcgcgaactc
      601 atgccgcggg cgatgttcct gatcccgggg gtcggggccc agggcgggcg cccggaagat
      661 ctcggcgcag ctttcgtcgc cggcagggca ggcgcgatgg tcgccgcttc gagatcgatc
      721 gccggggccg atgaccccgc cgcagcagcg gaagagctga gggcggcggt ctgggccgtc
      781 tccggttgaa tccgcccgga tcggacgggt tcctgcttgc cgatccgcgg tagcggtaac
      841 ttttcgaccc cggtcagcct tccggcattc gccgtacggc cgccccttcg gtccaatgga
      901 agaaaagaag aagaactccc agatcgcccg gctcctggcc gcaatcgcgc tcgtcggcgc
      961 ggtgctcatc ctgatcgtgg tgatttcagg gaactcgggg agcgatgaca agggcaatac
     1021 gaagaaggcg aacccggcca aacagcaggc caagaagaag ccgaagacga ccgccaagac
     1081 ttacgaagtc aaggaagggg acaccctgac caccatcgcg cacaagacgg gggtttccgt
     1141 tcagcggatt gaagcgttga accccgacct tgatccccag gccctccagg tgggccagga
     1201 actcaagctg cgttgacggg tcggccccgg ctcgcactcc tgctcctgct cctggccgtc
     1261 tggggggcag ggaccgccac ggccacggcc gcgtcggctc ccgaacgggg tccgaagctc
     1321 gacgccaagg cctggatcgt gatcgacgcc cggaccggtc agccgctggc cggccacgcg
     1381 gtgaaccgtc acctgggaat ggcttcgacc acgaagatga tgaccgccta cctgtcggtc
     1441 aggaaccttc ctttccggaa aagggtcaag gccggcaaat atgacccgga tccggccgaa
     1501 tcgctgatgg gtctggagcc gggccaggtc gtttcggtcc gtgacctgct ctacggactg
     1561 atgatgctga gcggaaacga tgcggcggtc accctggcca aggccgtgtc cgggagcgag
     1621 aagaagttcg tcaggttgat gaaccggacc gcgaggcaac tcggtctgga cgacacccat
     1681 tacgacaatc cggtcggtct cgacggtccc agccattaca cgtcggcagc cgacctggcg
     1741 aaactcggcc ggatcgttat gggcatgccg aggctgagga aaatcgccgg ttcgcggaag
     1801 gcgaagctga ccagttatcg cccgccggtc accatagaaa ccaccgacag cttcctgcgc
     1861 gacaacgggt gggcccgggg aatcaagacc ggccacacgc tcggcaccgg ttacacgctg
     1921 gcctcggacg ggcgccgcaa ggcgaccgag ctgatcggcg cggtcatcgg cactccgacc
     1981 gaagaggccc ggaacgacga aacggtccgg ctgctcgact gggggttcga ccagtacacg
     2041 aagcgggttc cggtcaaggt ggacaagccg gtggccatgg tgccggtgcg ctacgaggac
     2101 caggacctgc ccgtgaaatc gaggcggcgg gtccggatcg gagtgcggaa gggggagtcc
     2161 ctcacggtcg tgaccgacct gcccggagag gtggaaggac cgatcccggc
//
LOCUS       DAOTIP010000357         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_58903_length_2200_cov_2.887179, whole genome shotgun sequence.
ACCESSION   DAOTIP010000357 DAOTIP010000000
VERSION     DAOTIP010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58903_length_2200_cov_2.887179"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="P5138_07275"
     CDS             complement(<1..1417)
                     /locus_tag="P5138_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60417.1"
                     /translation="MVTGLSVAGYVALFTGGPRGPFRDFSRLRPRSTVVWPVAAAVGV
                     FFVFGAPVILSGSATFAGYIKLDDTSTWLAFTDHVLAHGHSVGGLAPSSYEATIQINL
                     SAGYPLGAFIPLGVGHELTGQDSAWLFQPYMALMAAFMSLVFYELAAPVIRDHRLRAA
                     AIFIAAQPALLVGYAFWGGIKEVATALLVSILAAGSVELARDPKPRSAVVVPILAGAA
                     LIGVMGAGGLPWLAAILGGAAFLMLATLWKARRDGGEISGPVKAMLVCGFVALAGIAV
                     IGLPTVFAGGQLFSPNQGPLTSSEEMGNLIRPLSLAQYPGPWPVGDFRVEPVSSLASL
                     LTSILVFATVVAAGFGLVAALVRRAWGLLLLTFGTGLGSFLVWLVGSPWVQGKALATG
                     SASFLILAMAGIGYLLGAGSKAEVEGEVEGRSRPFAGISTTGLRAIGAVLLIVPCGIL
                     VSSALAYHETWLAPKAQLAELS"
     gene            complement(1603..>2200)
                     /locus_tag="P5138_07280"
     CDS             complement(1603..>2200)
                     /locus_tag="P5138_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60418.1"
                     /translation="VSGATSTVKPEAPKMGAKQRAMVLGFAAVIVGVAVWGLTACNRD
                     GDPSSASTEVSMVSGDARIVSEDDLSGLTSDVGHQVFWAGPQADTQLEFSSDDTGNVH
                     LRYLTGGADAGDPNQSFLNIGSYPFAGAYAATRKLANGPNVVKVTEHGGVGFYDPANP
                     YSVIIAWPNQPDLQVEVYDPEKNRALEFVRSGDIVPVS"
BASE COUNT          420 a          727 c          768 g          285 t
ORIGIN      
        1 ccgagagttc ggccagctgc gccttcgggg cgagccaggt ctcgtggtag gcgagcgcgc
       61 tcgaaacgag gatgccgcag ggcacgatta gcagcaccgc cccgatcgct ctcagtccgg
      121 tcgtggaaat tcccgcgaaa ggcctcgacc taccctcgac ctcaccctct acctcagcct
      181 tcgatccggc tccgagcagg tagccgatac ccgccatcgc caggatcagg aaggatgccg
      241 agccggtggc gagcgccttg ccctgaaccc agggcgagcc gaccagccag accaggaatg
      301 atccgaggcc ggtcccgaag gtgagcagca gaagtcccca ggcccggcgg accagtgcgg
      361 cgaccaggcc gaagccggcg gcgaccaccg tcgcgaagac cagaatcgag gtgagcaggc
      421 tggccaggct cgagaccggc tcgacccgga agtccccgac cggccaggga cccgggtact
      481 gggcgagact gagcggccgg atcaggttgc ccatctcctc cgagctggtc agcggcccct
      541 gattggggga gaagagctgc ccgccggcaa agacggtcgg caggccgatc acagcgatcc
      601 cggccagggc gacaaacccg cagacgagca tcgccttgac cggtccggag atttcgcctc
      661 cgtcccggcg ggctttccag agggtcgcca gcatgaggaa agcggcaccg ccaaggatcg
      721 ccgccagcca gggcagcccg cccgccccca tcaccccgat cagggcggca ccggcgagga
      781 tcggcacgac cacggcgctg cggggcttcg gatcgcgggc cagctcgacc gaccccgcgg
      841 ccaggatcga gaccagcagg gcggtcgcca cttccttgat cccgccccag aatgcgtagc
      901 cgaccagcag ggccggctgg gcggcgatga agatcgcggc cgcccggagc cgatggtcgc
      961 ggatgaccgg ggcggccagc tcgtagaaga ccagcgacat gaacgccgcc atcagggcca
     1021 tgtagggctg gaagagccag gccgagtcct ggccggtcag ttcgtggccg accccgagcg
     1081 ggatgaaggc gccgagcgga tagccggccg agagattgat ctggatcgtc gcctcgtaac
     1141 tcgaaggcgc cagcccgccc accgaatgac catgggccag cacgtgatcg gtgaaggcga
     1201 gccaggtcga ggtgtcatcg agcttgatgt agccggcgaa ggtcgccgaa ccggaaagga
     1261 tgaccggggc gccgaaaacg aagaacacgc cgaccgcggc cgcgaccggc cagaccaccg
     1321 ttgaacgcgg gcgaagacgg gaaaagtccc tgaacggtcc ccgggggccg ccggtgaaca
     1381 gggccacgta tccggcgacc gagaggccgg tgaccacgcc ggtggtgaac tcggccagac
     1441 ccgaaaagca ggtgacgatt ccgcccgtcg cgatgatcgc ggcgaagccg gtggcaggca
     1501 gcagggtcca cggcaggatg cgcccgcgga cgagttcggc cagcagaccg agaccgacgc
     1561 tcagcgccag caggacgagg gggaagatga cccaagcgta gatcaggaca cggggacgat
     1621 atcgccactg cggacgaatt cgagggcccg gttcttctcc gggtcataca cctcgacctg
     1681 caggtccggc tggttcggcc aggcgatgat cacgctgtag gggttggccg ggtcgtagaa
     1741 gccgaccccc ccgtgttccg tgaccttgac cacgttcggg ccgttggcga gcttccgggt
     1801 cgccgcgtag gctccggcga acggatagct gccgatgttg aggaagctct ggttggggtc
     1861 acccgcgtcg gctccgccgg tcagatacct gagatggacg ttgccggtgt cgtcggagct
     1921 gaactcgagt tgggtgtccg cctgcgggcc ggcccagaag acctgatggc cgacatccga
     1981 agtgagtccg gacagatcgt cctcgctgac gatccgggcg tcaccggaaa ccatcgagac
     2041 ttcggtcgag gccgaggaag ggtcgccgtc acggttgcag gcggtgagcc cccagaccgc
     2101 gacaccgacg atgaccgccg cgaaaccgag caccatcgct cgctgtttgg cacccatctt
     2161 tggtgcctcg ggcttgactg tagaggtggc tccgctcaca
//
LOCUS       DAOTIP010000358         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59125_length_2194_cov_4.048153, whole genome shotgun sequence.
ACCESSION   DAOTIP010000358 DAOTIP010000000
VERSION     DAOTIP010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59125_length_2194_cov_4.048153"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="P5138_07285"
     CDS             <1..291
                     /locus_tag="P5138_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60419.1"
                     /translation="WDRQWEKSSAKHDDKYQFMEKLIKLPGFRSFDRNFDRLPESMRW
                     MVEKVVHDPDKPPAPKPEIPDELMETLKGRFRDDVAGLREWAGRDFDGWHEY"
     gene            318..1046
                     /locus_tag="P5138_07290"
     CDS             318..1046
                     /locus_tag="P5138_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRV60420.1"
                     /translation="MRRLFPDPGPTDLDAELDSYLPIENPPDHRPRVAVNMVTTLDGR
                     AAQGGNTKQLGSKTDTEHLLALRTRFDAVMIGGGTMRAERYGRIISRPENRKRREEEG
                     LSADALAVIVSGDLDLPFDAPLFTDGSGRVLIFTRKDEAPQTETPVDLITVEGLIDTS
                     DVLTHLRVEENVRAVLCEGGPRLFGQLVAGDLIDDFFLTTTPVMTGGDAPHILEGNLP
                     DRVDFRLESLLEADGDLFARYGRP"
     gene            1119..>2194
                     /locus_tag="P5138_07295"
     CDS             1119..>2194
                     /locus_tag="P5138_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60421.1"
                     /translation="MDFEAGSEVEGLLERVRSFMDEHIYPREAELHQALDDEVKAGVA
                     YPVELEQIREKAKAEGLWNLFLPDEEHGAGLNNWQYAFLCEEMGRSPLCAPMAFNSSA
                     PDTGNMEILLEHGTDEQKQKWLQPLLDGDIRSCFSMTEPDTAGSDPTGLAARADLVDG
                     EWVINGHKWFTSGAVGAEFAIAMVNTEDSDNPYANASMIIVPVDAPGFDLVRPVAVMG
                     HDKGPGHCEIRYEDCRVPEENLLGARGGGFLIAQDRLGPGRIHHCMRAIGTAERALEM
                     MCLRAADREAFGGPLAEKQFVQDFIAKSRMEIDQARLLSLNAAWKMDTVGKRAARQEI
                     SMIKVVAANMVMDVLDRAIQVHGAL"
BASE COUNT          425 a          692 c          719 g          358 t
ORIGIN      
        1 tgggaccggc agtgggagaa atcctcggcc aagcatgacg acaagtacca gttcatggag
       61 aagctgatca agcttccggg tttccgctca ttcgaccgta atttcgaccg tctgccggaa
      121 tcgatgcgct ggatggtcga gaaggtcgtc cacgacccgg acaagccccc ggccccgaag
      181 ccggagatcc cggacgagtt gatggaaacc ctgaagggcc ggttccgcga tgacgtggcc
      241 ggcctcaggg aatgggccgg ccgggacttc gacggctggc acgagtactg agcgccaggg
      301 ccgggtaggg gtctttgatg cgccgcctct tccccgaccc gggacccacc gacctcgacg
      361 cggagctcga ctcctatctt ccgatagaga atcctcccga tcatcgtccc agggtggccg
      421 tgaacatggt cacaaccctc gacgggaggg cagcccaggg agggaacacc aaacaacttg
      481 gatcgaagac ggacaccgag cacctgctcg ccctgagaac cagattcgac gcggtgatga
      541 tcggcggagg cacgatgcgg gccgagcgct acggtcgcat aatctcgcgc cccgaaaacc
      601 ggaagcgccg ggaggaagaa ggactttcgg ctgacgctct ggcggtgatc gtgagcggcg
      661 atctggacct ccccttcgat gcgcctctct tcaccgacgg aagcggccgg gtgctcatct
      721 tcacccgcaa ggacgaggcg ccacagaccg agaccccggt cgatctgatc acggtcgagg
      781 gactgatcga tacatcggac gttctcaccc atctccgggt cgaggagaac gtgcgggcag
      841 tgctctgcga aggtggccca cggctcttcg gtcagctggt ggctggcgac ctgatcgacg
      901 atttcttcct caccaccacc ccggtgatga ccggcgggga tgccccacac atcctggagg
      961 gaaaccttcc cgaccgggtc gatttcaggc tcgaaagcct gctcgaggcc gacggtgacc
     1021 tcttcgcccg gtacggccgg ccttgaggtt gcggctttct acgagacttc gcccgtctgg
     1081 tccgctgcaa cacttcggcg ggtagtagcg tccccggcat ggatttcgaa gccggttccg
     1141 aagttgaagg gctcctcgag cgcgtccgtt cgttcatgga cgaacacatc tatccgaggg
     1201 aagccgagct tcaccaggcg ctcgatgacg aagtaaaggc cggcgtcgcc tatccggtcg
     1261 aactggagca gatccgggag aaggcaaagg cggaaggtct ctggaacctc ttcctgcccg
     1321 acgaggaaca cggggcggga ctgaacaact ggcagtacgc cttcctttgc gaggagatgg
     1381 gccggagtcc gctctgtgcc ccgatggctt tcaacagttc cgcgcccgac accgggaaca
     1441 tggagatcct gctcgagcac ggcaccgacg agcagaagca gaagtggctc cagcccctgc
     1501 tcgacggaga cattcgaagc tgcttctcga tgaccgagcc ggacactgcc ggttccgatc
     1561 cgaccggcct cgccgccagg gcggacctgg tcgacggcga atgggtgatc aacggccaca
     1621 agtggttcac ttctggcgcg gtcggggccg agttcgcgat cgcgatggtc aacacggagg
     1681 actccgacaa cccgtacgcc aacgcatcga tgatcatcgt ccccgtcgat gcgccgggat
     1741 tcgatctggt caggccggtt gcggtgatgg ggcacgacaa gggcccgggc cactgcgaga
     1801 tccgatacga ggattgccgg gtccccgagg agaacctgct cggcgcccgc ggcggcggct
     1861 ttctgatcgc ccaggaccgg cttgggccgg gacgcatcca ccactgcatg agggcaatcg
     1921 gaacggccga gcgggcgctg gaaatgatgt gcctgagggc cgccgaccgc gaagccttcg
     1981 gagggccgct cgccgagaag cagttcgtgc aggacttcat cgccaaatcc cggatggaga
     2041 tcgaccaggc ccggctgctc tccctcaacg ccgcctggaa gatggacact gtcggcaagc
     2101 gggcggcccg ccaggagatc tcgatgatca aggtggtcgc cgccaacatg gtgatggacg
     2161 tgctcgaccg ggcgatccag gtccacggag cgct
//
LOCUS       DAOTIP010000359         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59127_length_2194_cov_3.669472, whole genome shotgun sequence.
ACCESSION   DAOTIP010000359 DAOTIP010000000
VERSION     DAOTIP010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59127_length_2194_cov_3.669472"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5138_07300"
     CDS             complement(<1..490)
                     /locus_tag="P5138_07300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRV60422.1"
                     /translation="MTSDSPESPAPEPGHSSEEPTAPWRDPAEIKRLDPMLEMLESSH
                     VSLSSVTDLEEARRVHVADSLSGLVCPEVRQARNALDLGSGGGFPGVPLAVLLPGCRF
                     TLIDSVGRKVEFLGEVISALGLENAQAVKARSEDLAAGEGREVFDLVTARAVAPLSVL
                     AEL"
     gene            complement(501..980)
                     /locus_tag="P5138_07305"
     CDS             complement(501..980)
                     /locus_tag="P5138_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRV60423.1"
                     /translation="MEASEWPADPAERVRLLVERVLDELDLDGEVEIDETEDVITATV
                     EGDEDYGLLIGRRGQTIDALQLLAFQAAFRGIRERKRVVVDAAGYRARREEVISERAD
                     RAAEKAIASGSEVELDPMSARERRIVHEHLKARPEVETFSSGDEPHRRVVVAPLISE"
     gene            complement(1008..1823)
                     /locus_tag="P5138_07310"
     CDS             complement(1008..1823)
                     /locus_tag="P5138_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRV60424.1"
                     /translation="MPVANILQPLIDVANGVLSFFHDDVGVSWGMAIILLTVCTRALL
                     IPLTYRQLKSMRALQAFQPQMKELQEKYKNDRQRLSQEMMKFYQENKINPLASCIPLL
                     AQLPVFITLFQALRGPLRDDICGQTAVACSQLTQAQIDANPAIHALYPFGESFLFIPD
                     LTDKATGGVLIALLILYVGTQLISGLISMTTGDRNQRMLMLALPFIFVPFIISFPAGL
                     ILYWITTNVWTIGQTYVINRVIPPPVMATPEGAVAAASTKAPPPPPKKKKRKR"
     gene            complement(1823..2098)
                     /gene="yidD"
                     /locus_tag="P5138_07315"
     CDS             complement(1823..2098)
                     /gene="yidD"
                     /locus_tag="P5138_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRV60425.1"
                     /translation="MKRIVLAPVRAYRRWISPLLPRRCKYEPTCSVYAIEAVERFGVI
                     RGTVLACWRILRCNPFSHGGFDPVRDEFTLKVGPMDPAVYHGEKEER"
BASE COUNT          425 a          662 c          720 g          387 t
ORIGIN      
        1 ccagctcggc caggaccgaa aggggagcga ccgcgcgggc cgtgaccagg tcgaatactt
       61 cgcgtccctc tccggcggca agatcctccg agcgggcctt gactgcctgg gcgttttcca
      121 gtcccagagc cgaaatcacc tcgcccagga actcgacctt gcggcccacg gagtcgatca
      181 gggtgaacct gcagcctggc aataagacgg ccaggggaac gccggggaag ccgccgccag
      241 agccgaggtc cagggcgtta cgggcctgac ggacctccgg acagacgaga cccgagaggc
      301 tgtcggccac gtgaacgcga cgggcttcct cgagatcggt gaccgaactc agcgagacgt
      361 ggcttgactc gagcatttcg agcatcgggt cgagccgctt gatttcagcc ggatcccgcc
      421 agggcgcggt cggctcttcg gatgaatgcc cgggttccgg tgccggtgat tcgggtgagt
      481 ccgaagtcac ccggccggct tcactcggag atcaggggag cgaccacaac ccgtcgatgc
      541 ggttcatcgc cggagctgaa agtctcgacc tcgggcctgg ccttgaggtg ctcatggacg
      601 atccggcgct ccctggcgct catcggatcg agttctacct cggagccgga ggcaatcgcc
      661 ttttcggcag cccggtcggc ccgctcggaa atcacttcct cgcggcgggc ccgatagcca
      721 gcggcgtcaa ccaccacccg cttgcgctcc cggatgcccc ggaatgccgc ctggaaagcc
      781 aaaagctgga gagcgtcgat ggtctggccg cgacggccga tcagcaagcc gtagtcctca
      841 tcaccctcaa cggtcgccgt gatgacgtct tcggtctcgt cgatttcgac ctcaccgtcg
      901 aggtcgagct cgtcgagaac ccgctcgacc agaagtctga ctcgttctgc cgggtcggcc
      961 ggccactcac tcgcttccat tgcccggagt ctagtcaggg caggcgctca gcgcttgcgc
     1021 ttcttcttct tcggaggcgg gggaggagcc ttggtggaag cggcggccac cgcaccttcg
     1081 ggagttgcca tcaccggcgg cgggatgacc cggttgatca cgtaggtctg accgatcgtc
     1141 cagacgttgg tcgtgatcca gtaaaggatc aggccggccg ggaaggagat gatgaaggga
     1201 acgaagatga acgggagcgc cagcatcagc atgcgctggt tgcgatcgcc cgtggtcatc
     1261 gagataaggc ccgagatgag ctgggttccg acgtagagga tcagcagggc gatcaggacg
     1321 ccaccggtgg ccttgtcggt cagatcgggg atgaagagga aggactcgcc gaaggggtaa
     1381 agcgcatgaa tagcgggatt ggcgtcgatc tgggcctggg tgagctgact gcaggcgacc
     1441 gcggtctggc cacagatgtc gtccctgagg ggaccgcgca gagcctggaa aagcgtgatg
     1501 aagaccggca actgggccag aagcgggata caggaggcaa gcggattgat cttgttctcc
     1561 tggtagaact tcatcatctc ctgcgagagg cgctgacggt cgttcttgta cttctcctgc
     1621 agctccttca tctgcggctg gaaggcctgc agagcgcgca tgctcttcaa ctggcggtaa
     1681 gtgaggggga tgaggagggc gcgggtgcag acggtcagaa ggatgatcgc catgccccag
     1741 ctgacgccga catcgtcgtg gaagaacgac agaaccccgt ttgccacatc aatgagtggc
     1801 tgaaggatgt tcgcgaccgg catcaacgct cttctttttc tccgtggtaa acggccggat
     1861 ccatcggacc gaccttcaaa gtgaattcat cccgcacggg atcgaagcca ccgtgactga
     1921 agggattgca gcgcaggatg cgccagcagg cgagaactgt accccgtatg acgccgaagc
     1981 gttctaccgc ctcgatcgcg tagaccgaac aggtgggctc gtacttacac cgacggggca
     2041 gtagagggga aatccagcgc ctgtaggcac gaaccggggc gagaacaatg cgtttcacgt
     2101 ggaacgcttg ccttcgtccc cgagagccag tacttccttg acgcaggcct cggctccggc
     2161 aagtccgtcc ttctcaatca cttctcctac gccg
//
LOCUS       DAOTIP010000360         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59232_length_2191_cov_3.983146, whole genome shotgun sequence.
ACCESSION   DAOTIP010000360 DAOTIP010000000
VERSION     DAOTIP010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59232_length_2191_cov_3.983146"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1403)
                     /locus_tag="P5138_07320"
     CDS             complement(21..1403)
                     /locus_tag="P5138_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV60426.1"
                     /translation="MSGTIVRGAWALTMGPGGAIREGAVAFDGDGRITAVGKAVELAA
                     ADPGATVVGDGNGIVLPGFVNAHTHLTEGLITGMGETAVLWEWFERVVNPSGINITRD
                     QVELGTRLKAVEMLESGITTVNDMCCHRNIGSLASLGAVDGLSAMGMRGVVCFGAEDM
                     YDGAPAADEFMAEHEALADRISSEDMIDFRIGVGTVLGMSDELFKRTIEAAAANDWAI
                     HTHLAEVREEITESTLLHGGSTIQHADRAGMLDHEVIAGHCIWCSENDIGLLASSDVA
                     VAHNPVANMILASGVCPVPRLLREGIRIGIGTDGAASNDSQDMFGAIKSAALLQKVHH
                     LQAVSMTAPQVMEMATIGGAKALGMEHEIGSLEPGKRADVVLLDGNGPELAVLHDPFQ
                     QIVYGATPRSVSEVWVDGELRVTGAQTVGVDRSALAAEARESARDLAIRGDMKGESAY
                     AGPLDFEEGK"
     gene            complement(1400..>2191)
                     /locus_tag="P5138_07325"
     CDS             complement(1400..>2191)
                     /locus_tag="P5138_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60427.1"
                     /translation="AAAVLPPLLFAIFVLAVWQLYTVVGNIRESTLPPPTDIAKSIWE
                     NRTLLIDNAWVTIKEILIGFVFAILLGVGLAVLIRSSRTIERAIYPWLVVSQMVPIPA
                     IAPLLIIWFGFELQPKVIVIMLVSFFPIAVNTIDGLRAAEPELLNLLKTLKANAWQRF
                     RKAQLPAALPFLFSGLKVAAALSVIGAVFAEWVGASEGLGYLILVLNNQTATTDMFAV
                     IFTLALIGILLFLIVTLLERLLLPWYYESRESEGEGLHLEVEEKA"
BASE COUNT          383 a          725 c          713 g          370 t
ORIGIN      
        1 tcactggttt ccgacaccct ttacttccct tcttcgaaat ccagggggcc ggcgtaggcc
       61 gactcgccct tcatgtcacc gcgaatcgcc aggtcccggg cagactcccg ggcctcggcg
      121 gcaagtgccg aacggtccac gccgacggtc tgggccccgg tcacccggag ctctccgtcg
      181 acccagactt cggagacgct gcgcggagta gcgccataga cgatctgctg gaagggatcg
      241 tgaagcacgg ccagttccgg gccgttgccg tcaagcagga ccacgtcggc ccgctttccc
      301 ggctcgaggc tgccgatctc atgttccatt ccgagggcct ttgccccgcc gatggtcgcc
      361 atctccatca cctgtggcgc ggtcatcgaa accgcctgca gatggtggac cttctgcaac
      421 agggcggccg acttgattgc gccgaacatg tcctgggaat cgttgctggc ggcgccgtcg
      481 gtgccgatcc cgatccggat gccttcgcgg agcagacgcg ggaccgggca gaccccggag
      541 gcgagaatca tgttcgcgac cgggttgtgc gcgaccgcca cgtcactgga tgcgagcagg
      601 ccgatgtcat tctccgaaca ccagatgcaa tggccggcaa tcacttcgtg gtcgagcatc
      661 ccggcccggt cggcatgctg gatggtcgag ccaccgtgga gcagggtcga ctcggtgatc
      721 tcctcccgca cctcggcgag atgggtatgg atcgcccagt cgttggccgc cgcggcctcg
      781 atcgtccgct tgaagagttc gtcactcatg ccgagcacgg tgccgacccc gatccggaag
      841 tcgatcatgt cttcggagga gatccggtcg gccagcgcct cgtgctcggc catgaactca
      901 tcggcggccg gtgccccgtc gtacatgtcc tcggccccga agcagaccac gccacgcatg
      961 cccatggccg agaggccgtc gaccgcgccg agcgaggcca gcgacccgat gttccggtgg
     1021 cagcacatgt cattgaccgt ggtgatgccg gattcgagca tctcgaccgc cttcagacgg
     1081 gtaccgagct cgacctggtc cctggtgatg ttgattccgg aggggttcac gacccgctcg
     1141 aaccattccc agaggactgc cgtctcaccc atcccggtga tcaggccctc ggtcaggtgg
     1201 gtgtgggcgt tcacgaatcc gggcaggacg atgccgttgc cgtcaccgac cacggtcgcc
     1261 ccgggatccg ccgccgcaag ctccaccgcc tttccgacag cggtgatcct gccgtctccg
     1321 tcgaaggcga ccgcgccttc gcggatcgcg ccccccggcc ccatggtcag ggcccaggcg
     1381 ccgcggacga tcgtgccgct catgccttct cctcgacttc gaggtggagg ccttcccctt
     1441 cgctctcacg cgactcgtag taccagggga gcagcagccg ttccagcagg gtgacgatca
     1501 ggaaaagcag gatgccgatc aaggccagcg tgaagatcac agcgaacatg tcagtggtcg
     1561 cagtctggtt gttcaggacc aggatcaggt agccgaggcc ttccgacgcg cccacccatt
     1621 cggcgaagac cgcgccgatc accgagagcg cggcggcgac cttcagcccc gagaagagga
     1681 agggcagggc ggcaggcaac tgcgccttgc ggaagcgctg ccaggcgttc gccttcaggg
     1741 tcttcagcag gttgagcagt tccggctcgg ccgccctgag gccatcgatc gtgttgaccg
     1801 cgatcgggaa gaagctgacc agcatgatga cgatcacctt gggctggagt tcgaaaccga
     1861 accagatgat caggagcgga gcgatggccg ggatcggcac catctgggag acgaccagcc
     1921 agggatagat cgcccgttcg atcgtgcggg aactgcggat caggaccgcc agaccgaccc
     1981 cgagcaggat cgcgaagacg aagccgatca ggatttcctt gatggtgacc caggcgttgt
     2041 cgatcagcag ggtgcggttc tcccagatcg acttggcgat gtcggtcggc ggcggcaggg
     2101 ttgactccct gatgttgccg accaccgtgt agagctgcca gacggcgagc acgaagatcg
     2161 cgaagagcag cggcgggagc accgccgcgg c
//
LOCUS       DAOTIP010000361         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59270_length_2190_cov_3.977049, whole genome shotgun sequence.
ACCESSION   DAOTIP010000361 DAOTIP010000000
VERSION     DAOTIP010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59270_length_2190_cov_3.977049"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="P5138_07330"
     CDS             <1..202
                     /locus_tag="P5138_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60428.1"
                     /translation="LDTGRPADLFCCFRPLGSSQNGPLESIEYGDIPGIAAVVIDGEV
                     RALRSRNTPAPDGACEIKEKNG"
     gene            195..776
                     /locus_tag="P5138_07335"
     CDS             195..776
                     /locus_tag="P5138_07335"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HRV60429.1"
                     /translation="MAEVIVPAREARTMEVKKGQTLEIVDLEGQQVGDFTAWLASDPN
                     EYLSPSHTVSSNGKLVPEVGDNILSNHRTPLLKILRDDVGRHDLVVPCCDPERYAIDY
                     DAPDHGSCLLSLQTATEGMGLPLRGEMCWNVFMNNQVEDGKIVTYEPPHPAGSTIAME
                     VLEDMTVALSACPQDISAVNAFNPTPMALRTSD"
     gene            786..1442
                     /locus_tag="P5138_07340"
     CDS             786..1442
                     /locus_tag="P5138_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRV60430.1"
                     /translation="MPRTSADEVAEAARILTGAGLVEAFGHVSARDGDGFLITSTRPL
                     GAAGGEDVHRMDSQGKAPEDASDVPLETPMHAAVYAARDDVNAICRTHSPAAVRAGAE
                     GEVPPLVHGLGGLAGEVAYSGRTDLVADEAAGREVAGDLGEANCLLIRGNGAIATGRN
                     MAEAVVRARYLEERCLAGNGLEQEAGWRADLLGPRARWFEKETTRAFAWLRWRYGGDS
                     "
     gene            1513..>2190
                     /locus_tag="P5138_07345"
     CDS             1513..>2190
                     /locus_tag="P5138_07345"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV60431.1"
                     /translation="MKFTTSWKKIMLLLAATMALAVGVAACGSSDDDSGGSGSGSSGG
                     TKNATLVLDFIPGAVHAGIYEAMQKGYYEDNGINLKIIEPTSTADTLKLIDAGKADFG
                     IADGIDLATQISDGREAQGIMALLQRPPGGLITLKDDNITDPKQLEGKTVGITGVPSD
                     TAILNTVMKDAGGNPDDANVVTIGFNGVQSLEAGKVSAFTGFIPADGVQVEADGYPDD
                     VRAVVRRR"
BASE COUNT          420 a          687 c          733 g          350 t
ORIGIN      
        1 gctggacacc ggccgccccg ccgatctctt ctgctgcttc cgtcctctgg gttcttccca
       61 gaacgggccg ctggaatcaa tcgaatacgg cgacatcccc gggatcgccg cggttgtgat
      121 cgacggcgag gtgagggcac tcaggtcgag aaacacgccg gctccggacg gggcctgtga
      181 aatcaaggag aagaatggct gaagtgatcg tcccggcaag ggaagcaagg accatggaag
      241 tgaagaaggg ccagaccctg gagattgtgg atctcgaagg gcagcaggtg ggggatttca
      301 ccgcctggct cgcctcggac ccgaacgaat acctctcgcc cagccacacg gtctcgtcga
      361 acgggaagct cgttcccgag gtcggagaca acatcctgtc gaaccaccgc acccctctct
      421 tgaagatcct tcgcgacgac gtcggacggc acgacctggt tgtcccctgc tgcgacccgg
      481 agcgctacgc gatcgactac gacgcaccgg accacggctc ctgcctgctc tccctgcaga
      541 ccgcgaccga gggcatgggc cttccgttgc gcggcgagat gtgctggaac gtcttcatga
      601 acaaccaggt ggaagacggg aagatcgtca cctacgagcc gcctcatccg gccggttcca
      661 cgattgcgat ggaggtgctg gaggacatga ccgtggccct ttcggcctgc ccccaggaca
      721 tctccgcggt caacgccttc aacccgaccc cgatggcgct gaggaccagt gactgagtga
      781 ccggagtgcc gcgaacatcc gccgacgaag tggccgaagc agcccggatc ctcaccggag
      841 ccgggctggt cgaggcattc ggacatgtct cggctcgcga tggcgacggt ttcctgatca
      901 cctcgacccg gccgctcggc gccgccggcg gcgaggatgt gcacaggatg gattcgcagg
      961 gcaaagcccc ggaagatgct tcggacgtgc cgctggagac cccgatgcac gccgccgtct
     1021 acgcggcccg ggatgacgtg aacgcgatct gccgcaccca ttccccggct gccgtccggg
     1081 ccggggccga gggcgaggtg ccgccgctgg tccatggcct cggcggactt gccggtgagg
     1141 tggcttactc ggggcgtacc gacctggttg ccgacgaggc ggccggcagg gaggtcgcag
     1201 gagatctcgg ggaggcaaat tgtcttttga tccgcggaaa cggcgcgatc gccactggcc
     1261 ggaacatggc cgaagcggtc gtccgggccc gctatctgga ggagaggtgc ctggctggaa
     1321 atgggctcga acaggaggcc ggttggaggg ccgacctgct ggggccccgg gccaggtggt
     1381 tcgaaaagga aacgacccgg gccttcgcct ggttgcgatg gaggtatgga ggagattctt
     1441 gacggatcgc tgtgagctcg tcccggccag acgccgagcg ggtgtttcga actgactaac
     1501 tagagaggac ccatgaagtt cacaaccagt tggaaaaaga tcatgttgct gctggcggca
     1561 acgatggccc ttgcggtcgg cgtcgcagcc tgcggttcaa gtgacgacga ttcgggcggt
     1621 tccggatctg gcagttcagg cggcaccaag aacgcgaccc ttgtgctcga cttcatcccg
     1681 ggagcggttc acgccgggat ctacgaggcg atgcagaagg gctactacga ggacaacggc
     1741 atcaacctga agatcatcga gccgacctcg accgccgaca ccctgaagct gatcgacgcc
     1801 ggcaaggccg atttcggcat cgccgatggc atcgacctgg cgacccagat cagcgacggc
     1861 cgcgaagccc agggcatcat ggcgctgctc cagcgtccgc ccggtggcct gatcaccctg
     1921 aaggacgaca acatcaccga cccgaagcag ctcgagggca agactgtcgg aatcaccggt
     1981 gtgccttcgg acacggcgat cctgaacacg gtgatgaagg acgcgggcgg caaccccgac
     2041 gacgccaatg tggtgacgat cggcttcaac ggggtccaat ccctcgaagc cggaaaggtg
     2101 tccgccttca ccggattcat cccggccgac ggcgtccagg ttgaagccga cggatacccc
     2161 gatgatgttc gagccgttgt ccggcggcgg
//
LOCUS       DAOTIP010000362         2189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59308_length_2189_cov_3.218369, whole genome shotgun sequence.
ACCESSION   DAOTIP010000362 DAOTIP010000000
VERSION     DAOTIP010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59308_length_2189_cov_3.218369"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="P5138_07350"
     CDS             <1..624
                     /locus_tag="P5138_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HRV60432.1"
                     /translation="ALVAEVVAGEIARHAGLPVPELVLVDIAPELGQAEPDPEIQELI
                     EASPGLNLGMDFLPGALPFALPTDEEFDPELAADIVFFDALVTNIDRTPRNPNLLTWH
                     GRTWLIDHGAAFYRQHGAVPLAETATSPTPVLRDHVLLPVAGPLAEAGERLTAAVEGA
                     IDPALAAVPEGWLGDDPEARREDFALFLQRRLGARDVFIAELEGEDG"
     gene            617..1000
                     /locus_tag="P5138_07355"
     CDS             617..1000
                     /locus_tag="P5138_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HRV60433.1"
                     /translation="MAEPRPFSYAVLRVVPCIERGERLNVGLALFCRQHDFLDLEVNL
                     DRSRLAAIAPDLDPAPVEERLAVIRRVIEGDPAAGSLAGLDPSDRFGWLTAPSSTIIQ
                     SSEVHTGLTTDPAAELKRLFESLVL"
     gene            997..>2189
                     /locus_tag="P5138_07360"
     CDS             997..>2189
                     /locus_tag="P5138_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV60434.1"
                     /translation="MILDGAREAFREDPSRLAVVDDSGSSTWTGFMAEIGQAAADIGA
                     SPTGAPVEIRLPSGREFLTRFLAAAQLRRPACTLHADWAGPELEAATAAARSFRPVVP
                     PAEDDDPVFCIGFTSGTSGRPKPFARRSMSWISSFEPAGEMFSIEAGDTVYLPGSLQH
                     SHFLFGAVLGLNRGASVRMFERFDRAALAEELNGVSRGILYLVPTMLFTLDELDVDPL
                     PGLHSLVISGAKMEPHHWQIARRLFPGASVSEIYGASELSFVTVNSGGSDRSDPGFVG
                     PPFPGVEIEIRSDGDGPGLVFVRSPYLFDGYFDESGRQSPIGPDGFMTVGDVGVLGEE
                     GLSIVGRASNMLITGGKNVHPEEVESRLVEHGSVSECVVLGLPDPRWGEEIVAFVVSS
                     GEGAGP"
BASE COUNT          344 a          744 c          714 g          387 t
ORIGIN      
        1 gcgctcgtgg ccgaagtggt ggccggtgag atcgcgcgcc acgcggggct tccggtgccg
       61 gaactggtgc tggtcgacat cgcgccggaa ctgggccagg ccgagcccga ccccgaaatt
      121 caggaactga tcgaagcctc gccgggactc aatctcggca tggacttcct gcccggggcg
      181 ctccccttcg cgctgccgac ggatgaggaa ttcgatccgg aactggcggc cgacatcgtc
      241 ttcttcgacg cgctcgtcac caacatcgat cgcactcccc gcaaccccaa cctgctgacc
      301 tggcacggac gcacctggct gatcgaccac ggcgccgcct tctatcggca gcacggggcc
      361 gtaccccttg ccgaaacggc cacctccccc accccggtcc ttcgcgacca cgtgctgctg
      421 ccggtcgccg ggccgctggc ggaagcaggc gaaaggctga cagccgccgt ggagggagct
      481 atagaccccg ccctggcggc ggtgccggaa gggtggctcg gggacgaccc ggaggcaaga
      541 agagaagact tcgccctctt tcttcagcgg cgactcggag cccgggacgt cttcattgcc
      601 gaactggaag gcgaggatgg ctgaaccgcg ccccttcagt tacgcggtgc tccgggtcgt
      661 tccctgcatc gaaagggggg agcggctcaa cgtggggctt gccctgttct gccgccagca
      721 cgactttctc gatctggagg tcaacctgga tcgttcccgc ctcgccgcaa tcgcccccga
      781 cctggatccg gccccggtcg aggagaggct ggcggtgatc cgccgagtga tcgagggaga
      841 tccggccgcc ggctcgctcg ccggactcga cccttcggac cgtttcggct ggctgaccgc
      901 cccctcctcg acgatcatcc agagctccga ggtccacacc ggactgacca ccgacccggc
      961 ggccgaactg aaacggctct tcgagagcct cgtgctgtga tcctcgacgg ggcccgcgag
     1021 gcgttccgtg aagatccctc gcggctcgcc gtggtcgacg attcgggttc ctcgacctgg
     1081 accgggttca tggccgaaat cgggcaagcc gcagccgata tcggagcgag cccgaccgga
     1141 gcgccggtcg agatccggct tccatccggc cgggagttcc tgacgaggtt cctggccgca
     1201 gcccaactga gacgcccggc ctgcaccctt cacgcagact gggccggacc tgagctcgaa
     1261 gcagccaccg cggccgcccg gagcttccgg ccggttgttc ccccggccga agacgacgac
     1321 ccggtcttct gcatcggctt cacttccggc accagcggcc gaccgaagcc gttcgcccga
     1381 cgatcgatgt cgtggatctc aagctttgaa ccggccggcg agatgttctc gatcgaagcc
     1441 ggtgacaccg tctacctgcc cgggtcgctt cagcattcgc attttctttt cggggcggtg
     1501 ctgggcctga atcgcggggc ttcggtgcgg atgttcgagc gcttcgaccg tgcggcgctg
     1561 gccgaagagc tgaacggggt gtcgcgggga atcctctacc tcgttccgac catgcttttc
     1621 accctcgatg aactggatgt cgatcccctc ccagggcttc actctctggt catatccggc
     1681 gcgaaaatgg aacctcacca ctggcagatc gcccggcgcc tcttccccgg ggcctcggtg
     1741 agcgagatct acggagcctc cgagctcagc ttcgtgacgg tgaattccgg gggcagcgac
     1801 cggtccgatc ccgggttcgt cggcccgccg tttcccggag ttgaaatcga gatcagatca
     1861 gatggagatg ggcccggact cgtttttgtc cgcagcccgt acctcttcga cggctacttc
     1921 gacgagtcag gccgccagtc tccgatcggg ccggacggtt tcatgacggt cggtgatgtc
     1981 ggggtactcg gcgaggaggg actctcgatc gtcggccgcg cctcgaacat gctgatcacg
     2041 ggcggcaaga acgtccaccc ggaagaagtc gaatcgaggc tggtcgagca cgggtcggtg
     2101 tcggaatgcg tggtcctcgg gttgccggat ccacgctggg gcgaggagat cgtcgccttc
     2161 gtcgtttctt cgggcgaagg agccggacc
//
LOCUS       DAOTIP010000363         2188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59361_length_2188_cov_2.542429, whole genome shotgun sequence.
ACCESSION   DAOTIP010000363 DAOTIP010000000
VERSION     DAOTIP010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59361_length_2188_cov_2.542429"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2188
                     /locus_tag="P5138_07365"
     CDS             <1..>2188
                     /locus_tag="P5138_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRV60435.1"
                     /translation="TVSLRLRARDGNGLKGEDRRSIGARADKDLVSGFPKSIGTEMSA
                     APTFVDLGGKRQLDLVFGTMDGSVSALEPDGSQAPGFPVYTWKLREVDPRNPQNFDSE
                     AYNSVSALRDARDPISGIAVGDLDGNGAQSIVATTTSGWIYVWNASGKLRQGFPVHGD
                     DQYNTLPVPTPNSGCRRCRLPIRGNWSAPALGNLQDNGKLDILMSSYDGHLYAFQANG
                     DPVPGWPVKVELPQSMKDSIGANDYISDPKLMMTPAVGNVEGDGADEVFLPSFECDEG
                     DGKSFAYGIHSDGNNNPGGAYLTGWPVTMNTIAGCYSQSIDFVQEGANAASIGDFDGS
                     GTERIAITPVAGAPVIFNADGSVFKNMLPTCPSDACDGYPPYYGSDAITVGVTSQSAI
                     GDLTGDGLPEFMQPVAGGATLTTAFGDAGQAALAHVFDQAWNPITGQTLANFPTDQDG
                     FPFYSSPVVANLTDDGNRSMIAANDSYWIHARGPDGKEAPGFPKWTGQWTSFGGAVGD
                     PKLDGQQYLAYGTREGQLFLWKVGGKPSMNDQWWHYRHDEHNSGRYGNDTRPPAGVKF
                     VVKRSKKSAKVTWRASGDDGVSGGKVAAVQVYKSSKKISLSQLGSRIKSPQIAKPNGQ
                     QKVSVKLAPKKSLYIAVRQRDADGNWSALSRFKIKPFKSFNLKKQKRICRKKFKTRGK
                     HGAAKRKAKKKTRTGPSRNPIVFVWNYLKQVVAELRKVIWPNRKQMV"
BASE COUNT          423 a          762 c          687 g          316 t
ORIGIN      
        1 cacggtgagc ctccgcctgc gggcccgtga cggcaacggc ctcaagggcg aggaccgccg
       61 ctcgatcggc gcccgtgccg acaaggacct cgtgtccggc ttcccgaagt cgatcggcac
      121 cgagatgagc gccgccccga ccttcgtcga cctcggcgga aagcgccagc tcgacctggt
      181 cttcggcacc atggacggct cggtgagcgc cctcgagccg gacggctcac aggctcccgg
      241 cttccccgtc tacacctgga agctccggga agtcgacccg cgcaacccgc agaacttcga
      301 cagcgaggcc tacaactcgg tttcggccct gcgcgacgcc agggacccga tcagcggcat
      361 tgcggtcggc gacctggacg gcaacggcgc ccagtcgatc gtcgcgacca cgaccagcgg
      421 ctggatttac gtctggaacg cctccggaaa gctgcgccag ggcttcccgg tccatggcga
      481 cgaccagtac aacaccctgc cagtcccgac cccgaactcg ggctgccggc gctgccgcct
      541 gccgatccgc ggcaactggt ccgccccggc gctgggcaac ctgcaggaca acggcaagct
      601 ggacatcctc atgtccagct atgacggtca cctctacgcc ttccaggcga acggcgatcc
      661 cgtgccgggc tggccggtca aggtcgagct gccgcagtcg atgaaggatt cgatcggagc
      721 caacgactac atctccgatc cgaagttgat gatgaccccg gcggtcggca acgtcgaggg
      781 tgacggcgcc gacgaggtct tcctgcccag cttcgaatgc gacgaaggcg acggcaagag
      841 cttcgcctac ggaatccatt cggacggcaa caacaatccg ggcggggcgt acctgacggg
      901 ctggccggtg accatgaaca cgatcgccgg ctgctactcg cagtcgatcg acttcgtcca
      961 ggaaggcgcg aacgccgcct cgatcggaga cttcgacggt tccggaaccg agcgcatcgc
     1021 gatcaccccg gtcgccggcg cgccggtgat cttcaacgcc gacggcagcg tcttcaagaa
     1081 catgctgccg acctgcccgt cggacgcctg cgacggatac ccgccttact acggttcgga
     1141 cgcgatcacc gtcggcgtga ccagccagtc ggcgatcggc gatctgaccg gtgacgggct
     1201 gcccgagttc atgcagccgg tggccggcgg cgccaccctg acgaccgcct tcggcgatgc
     1261 cggacaggcg gcactggccc atgtcttcga ccaggcctgg aacccgatca cggggcagac
     1321 cctggcgaac ttccccaccg accaggacgg gttccccttc tactcctccc cggttgtcgc
     1381 caacctgacc gacgacggca accgctcgat gatcgcggcc aacgactcgt actggatcca
     1441 cgcgcgcggt ccggacggca aggaagctcc gggcttcccg aagtggaccg gccagtggac
     1501 ctcattcgga ggcgcagtcg gagatcccaa gctggatggt cagcagtacc tggcctacgg
     1561 cacccgtgaa ggccagctct tcctctggaa ggtcggcggc aagccgtcca tgaacgacca
     1621 gtggtggcat taccgccacg acgagcacaa ctctggccgc tacggcaacg acacccgtcc
     1681 ccctgccggc gtcaagttcg tggtcaagcg ctcgaagaag tcggccaagg tcacctggcg
     1741 cgcttccggc gatgacggcg tgagcggcgg caaggtcgcg gccgtccagg tctacaagtc
     1801 gagcaagaag atcagcctgt cgcagctggg cagccggatc aagtcgccgc agatcgccaa
     1861 gccgaacggc cagcagaagg tgagcgtgaa gcttgctcca aagaagagcc tctacatcgc
     1921 ggtccgccag cgtgacgccg acggcaactg gtcggccctg agtcgcttca agatcaagcc
     1981 cttcaagagc ttcaacctga agaagcagaa gcggatctgc cgcaagaagt tcaagacccg
     2041 tggcaagcac ggcgccgcga agcgcaaggc caagaagaaa acgcgcaccg gcccgtcgcg
     2101 caacccgatc gtgttcgtgt ggaactacct caagcaggtc gtcgccgagc tgcgcaaggt
     2161 gatctggccc aaccgcaagc agatggtc
//
LOCUS       DAOTIP010000364         2177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_59763_length_2177_cov_2.983506, whole genome shotgun sequence.
ACCESSION   DAOTIP010000364 DAOTIP010000000
VERSION     DAOTIP010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59763_length_2177_cov_2.983506"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /gene="ispG"
                     /locus_tag="P5138_07370"
                     /gene_synonym="gcpE"
     CDS             complement(<1..1180)
                     /gene="ispG"
                     /locus_tag="P5138_07370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRV60436.1"
                     /translation="MASKRQIFVGRVPVGGGAPVAVQTMTKTETANLQATMDQINQVA
                     EAGADIVRVAVPRDKDIEALKTIVKQSPIPIIADIHFNHTLALKAIEAGADCIRLNPG
                     NIGGREKVAEVAELANKRGTPMRIGVNSGSLPKHLHALERENPTEALVTAAVEFVDLM
                     EGLDFTNFKVSIKSTNVPNTIAANRLLSEKIPYPIHLGITEAGTKWSGSLKSAVGLGA
                     LLADDIGDTIRISLSTFHAEEEVKVAWEILKALKLREKGPVLIACPTCGRLQFDMDAV
                     VAEVEARLETYEEPIEVAVLGCAVNGIGEAKGADFGIAGAKNEGVVFAKGKAIKKVPT
                     SQLVETLFEQIDLSLDRGGEVEFDEKESAEGEAWVKKIEDENAGDLTPEKIARMEHEA
                     S"
     gene            complement(1245..>2177)
                     /locus_tag="P5138_07375"
     CDS             complement(1245..>2177)
                     /locus_tag="P5138_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60437.1"
                     /translation="ATALQAGIVVLLGLLRTEEAGSDQPFSVGALRTRVLGELGGSEI
                     SVGELGLALWAESRAGGEAIGEIDGLLQRATVKGLEKVPLEQLAWLSSGLSESAVLTG
                     DPSTGLVRDVKAELLSRLTPDGLSRDVHARRLGAAVPVASQFHLLHALCQMVRAGDPE
                     LREPADRLARGLLGLQREDGAWPGLIDPVRSSAAVIYPVLTVTQLAIAPIALRAAAEL
                     DLDIDFESTVGAGMKWTEGGNALGLELVHEADARIDRGILPKRTPGALGRGISSAARR
                     IRGQAFEPDQKQLIIDPDASSEDLGWVLEAWAGR"
BASE COUNT          338 a          721 c          707 g          411 t
ORIGIN      
        1 gggatgcctc gtgctccatg cgggcgatct tctccggggt gaggtcgccg gcattttcgt
       61 cctcgatctt cttgacccag gcttcgcctt cggccgattc cttttcgtcg aactcgacct
      121 cgccgccccg gtcgagcgag agatcgatct gctcgaagag cgtttccacc agctggctgg
      181 tcggaacctt cttgatcgcc ttgcccttgg cgaagacgac gccctcgttc ttggccccgg
      241 cgatgccgaa gtcggccccc ttggcctcgc cgatcccgtt caccgcgcaa ccgagcactg
      301 ccacctcgat cggctcttcg taggtctcca ggcgcgcctc aacctcggcc acgacggcgt
      361 ccatgtcgaa ctggagacgg ccgcaggtcg ggcaggcgat caggaccgga cccttctcgc
      421 ggagtttcag cgccttgagg atttcccagg cgaccttgac ctcttcttcc gcatggaagg
      481 tcgagaggct gatccggatc gtgtcgccga tgtcatcggc gagcagcgcg ccgaggccga
      541 ccgcgctctt cagcgatccg gaccacttgg tcccggcctc ggtgatcccg aggtggatcg
      601 ggtacggaat cttctccgaa agcagccggt tggcggcgat cgtgttcggc acattggtcg
      661 acttgatcga caccttgaag ttcgtgaagt cgaggccttc catcaggtca acgaactcga
      721 ctgcagcggt gaccagagcc tctgtcgggt tctcgcgttc aagcgcgtga aggtgctttg
      781 gaagggagcc ggagttgacc ccgatccgca tcggggtgcc ccgcttgttt gcgagctcgg
      841 ccacctctgc gaccttctcc cggccaccga tgttgccggg gttcagccgg atgcagtccg
      901 ccccggcttc gatcgccttg agggcgaggg tgtggttgaa gtggatgtcg gcgatgatcg
      961 ggatcggcga ctgcttgacg atcgtcttca gcgcctcgat gtccttgtcc cgcgggaccg
     1021 cgacgcggac gatgtctgct cccgcctcgg cgacctggtt gatctggtcc atcgtcgcct
     1081 gcaggttggc ggtttcggtc ttggtcatgg tctggaccgc aacaggggcc ccgccaccga
     1141 ccggaacccg gcccacgaat atctgtcttt tcgatgccac tgggaatgca ggctaccgga
     1201 gccctgaatc ggtcaggacg aagccagcgg gccgttggtg gctcctagcg gcctgcccag
     1261 gcttcgagta cccagcccag atcttcggaa gatgcgtccg ggtcgatgat cagctgtttc
     1321 tgatcgggct caaaggcctg gccgcgaatc cgccgggcag cggaacttat gccgcgtccg
     1381 agggccccgg gagtccgttt gggaaggatg ccacgatcga tccgggcgtc ggcctcgtgg
     1441 accagttcga gtccgagggc gttcccgcct tcggtccatt tcatgccggc cccgactgtg
     1501 ctttcaaagt cgatgtcgag atccagctcc gcggccgcgc gcagtgcgat cggggcgatg
     1561 gccagctggg tgacggtgag caccgggtaa atcacggcgg cgctcgaacg aaccgggtcg
     1621 atcagaccgg gccaggcgcc gtcctcacgc tggagcccga gcagtccccg ggccagccgg
     1681 tcggccggct cccgcaattc cgggtcgcct gcacggacca tctggcagag ggcatgaagc
     1741 aggtgaaact gactggcgac ggggaccgcg gcacccaggc gcctcgcatg gacgtcccgg
     1801 gacagcccgt ccggtgtgag acgggacagc agttcggcct tcacgtccct gaccaggccg
     1861 gttgacgggt ctccggtaag gaccgcgctc tccgaaaggc ccgaagagag ccaggcgagc
     1921 tgttccagcg ggaccttctc gagtcccttg acggtggctc gctggagcag tccgtcgatc
     1981 tcgccgatcg cctctccccc ggctcgcgac tcggcccaga gggccaggcc aagttctccg
     2041 acgcttatct ccgagccgcc aagctcaccg aggacacggg ttctcagggc accgacggaa
     2101 aagggttgat cggacccggc ttcctcggtc ctcagcagcc cgagcagaac cacgatgccg
     2161 gcctgaagcg cagtcgc
//
LOCUS       DAOTIP010000365         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_60150_length_2167_cov_2.802557, whole genome shotgun sequence.
ACCESSION   DAOTIP010000365 DAOTIP010000000
VERSION     DAOTIP010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60150_length_2167_cov_2.802557"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="P5138_07380"
     CDS             complement(<1..1468)
                     /locus_tag="P5138_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRV60438.1"
                     /translation="MKLRSRFVSALALLAGLVVAGLALTAPANAADQSLNYVAMGDSY
                     SAASGVLPPDPSAPPECLRSSLNYPHVLAKKLGADLTDVTCGAAETSDFTESQYQGVA
                     PQLDALADDTDLVTMTIGGNDSSVFIGAILSCGSLGAASLGQGSPCRDTFGDQYENTI
                     RGTTYPSLVEALNAVHAKAPKARVAILGYPWIMPEADGCFPQMPVASGDVPYLRSLQR
                     TLNDAVRRAARDTDSIYVNMNSVSNGHDACQPIGTRWIEPVLLGTNPVIVHPNALGES
                     KMAEEAFKSLSQETGNGASRARPQTTIRKVLVKGRKHQATFRFKSSKAGSTFRCRLDR
                     RKFRNCRSPKSYRNLKRGRHVFRVAAVDDHGRIDRSPAPYFDLEEERRLHTMLLILIR
                     KGVINAAHDVSDGGLWTALVEMALPRGLGFDVITDSEIREDAFLFGEGQGRAVVAVSD
                     EQETPFLDLMQASRVPFILLGHVTKGKLMVDDAPFGTID"
     gene            complement(1661..2068)
                     /locus_tag="P5138_07385"
     CDS             complement(1661..2068)
                     /locus_tag="P5138_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60439.1"
                     /translation="MAELEGRSLEIVRDGQNYAIVAVPTDDGSVQSVIVWAHADEAGN
                     VTLNSAEGRAWPKNLRKAGRATVTMMADGNPYEYVSIRGKLVGDTHEGADEDINMLAK
                     KYIDADEYPFRQPGEVRVKFTLEPEKVNYLKQG"
BASE COUNT          371 a          685 c          705 g          406 t
ORIGIN      
        1 catcaatggt accgaacggc gcatcgtcca ccatcaactt gcctttggtg acgtggccaa
       61 gcaggatgaa cgggacacga ctggcctgca tcaggtcgag gaagggggtc tcctgttcgt
      121 cgctcacggc gaccacggcc ctaccctgcc cttcgccgaa gaggaaggca tcctcgcgga
      181 tctcgctgtc ggtgatcaca tcgaagccga ggcctcgcgg cagggccatc tccacgaggg
      241 ccgtccacag cccaccatcg ctcacatcgt gtgcggcgtt gatcacgccc ttgcggatga
      301 ggatgagcag catggtgtgc aggcgacgct cttcttcgag gtcgaaatag ggcgccggac
      361 tgcgatcgat gcggccatgg tcgtcgaccg ccgcgaccct gaagacatgc cttccccgct
      421 tcagattcct gtagctcttc ggcgagcggc agttcctgaa cttcctcctg tccagccggc
      481 agcggaaggt cgagccggct ttgctcgact tgaacctgaa cgtggcctgg tgttttctgc
      541 ccttgaccag gaccttgcgg atcgtggtct gtggtcgagc ccgggatgca ccgttgccgg
      601 tctcctgtga gagcgacttg aacgcctcct cggccatctt cgactcgccg agcgcgttgg
      661 ggtgaacgat cacgggattg gtgccaagca ggaccggttc gatccagcgg gtgccgatcg
      721 gctggcaggc gtcgtggccg ttcgagacag agttcatgtt cacgtagatc gagtccgtgt
      781 cccgggcggc acgacggacc gcgtcgttga gggtccgctg cagggaccgc aggtacggga
      841 cgtccccgct ggccaccggc atctgcggaa agcagccgtc ggcctcgggc atgatccagg
      901 ggtaaccgag gatggcgacc cgggcctttg gcgccttcgc gtgaacagcg ttcagtgcct
      961 cgaccaggga tgggtaggtg gtcccgcgga tcgtgttctc gtactggtct ccgaaagtgt
     1021 cccggcaggg gctcccctgg ccgagagatg ccgcgccgag tgacccgcag ctgaggatgg
     1081 cgccgatgaa gacactgctg tcgttgccgc cgatggtcat ggtgaccagg tcggtgtcat
     1141 cggccaatgc gtccagctgc ggagccactc cctgatattg cgattcggtg aaatccgagg
     1201 tttcagcagc gccgcaggtc acgtcggtca ggtcggcccc cagcttcttc gccaggacat
     1261 gcgggtagtt cagggaggag cggaggcatt ccggcggtgc ggaagggtcg ggagggagca
     1321 cgccggacgc ggcgctgtag gaatcgccca tcgcgacgta gttgagcgac tggtccgcgg
     1381 cgttggcggg agcggtcaga gccagaccgg ccacgaccag accggccaac aaggcgaggg
     1441 cggacacaaa gcgactgcga agcttcatca aatctcccgg ggtagttggg aacaccagcg
     1501 ttcctgagtg ctccgtgact accccggggt ccgaacccca aaccgggtca gtccgaaccc
     1561 caaaccgggt cggacgggga tggcgaccgc cccgaccctg cagatcgcgg ccgcccctgc
     1621 actgctcccg gccccgcggc agaccgactg gacctcgcga ctaaccctgc ttcaggtagt
     1681 tgaccttctc gggctcgagg gtgaacttga ctctgacctc acctggctgg cggaagggat
     1741 attcgtccgc gtcgatgtac ttcttggcca gcatgttgat gtcttcgtcc gcgccctcgt
     1801 gcgtgtctcc gaccagcttg ccgcggatcg acacatactc gtagggattg ccgtcggcca
     1861 tcatggtcac ggtcgccctg ccagccttcc tgaggttctt cggccatgca cggccctcgg
     1921 cgctgttcag ggtcacgttg ccagcctcat cggcatgcgc ccagacgatc acggactgaa
     1981 ccgagccgtc atcggtcgga acggcgacga tcgcgtagtt ctggccgtcc ctgacgattt
     2041 cgagcgagcg accttcaagt tctgccattt gaatctcctc ttcggactgg ttggaattga
     2101 ctgatccggg accggaccga atctacagag gcggattcac cggcccgaaa gctcagccgg
     2161 caaggca
//
LOCUS       DAOTIP010000366         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_60249_length_2165_cov_1.713744, whole genome shotgun sequence.
ACCESSION   DAOTIP010000366 DAOTIP010000000
VERSION     DAOTIP010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60249_length_2165_cov_1.713744"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="P5138_07390"
     CDS             <1..2099
                     /locus_tag="P5138_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60440.1"
                     /translation="TKGNVMSEKGSRLEGGALTGEVIDSSRKVLTGGRNGTGVGLQQY
                     FTPPQVGELLAKVFNRDLPTLDLTAGDGGLLEWVSDEHRYGIEIDRDQVSNGNYAKHA
                     ITGDIQKVYPLLRGLGATWPQLVVNPPFGLDWKVPGIEDGKTGSSTLRAFQMMCRLTD
                     SYGQAAFICGRDRYIKEIAGRPEAEQVWAKIEVDDLFDGVELPVVLCLTVGELVHKTV
                     EGTVDLHVKKDELTMCWPRLVAEQDQRTGFQPVFAKIGQNRCESVFVTAAAEHGRRAT
                     GTERKGFDLSLTGQKIRVRLGEYSKVALAQGGYRELESVRNLNGLATSWVALNPKSWR
                     EIIGLSDQGKITIEPKLLEKISEVEVEVRRELVPLYSVRPQQRLGYLTDIENIVCRAD
                     APDYEFVAGESYPISTSSKIEEHDEERPYEKKDGTHEIRKFRTKRRVLEIRIGNHVFS
                     EAAADVEFLLDHFEIPDPGDIADQRPDEVAKALSVLDNLTETHDWAGKGLDWKGLHDP
                     ETGEWIQWQKEDLARAVVKGRAILGWEQGGGKSLGLLSLALAAIEYHRLPRQAMLVVP
                     QDLLPQWRREAKRFFDIDFEVIDTPAKARQVKKRMKRGEVDIEADQPDHPLCAQLGGI
                     EYKFASGTAESMLILSKKELREKGLKSPDAADAASYSAAEVDVEGMLGLQPGDGFALD
                     IDLLSSSVFSSNSFWN"
BASE COUNT          510 a          644 c          666 g          345 t
ORIGIN      
        1 aaacgaaagg aaacgtcatg agtgaaaaag gcagccgcct tgaaggtggc gccctgacag
       61 gcgaagtcat cgactcgtca aggaaggttc tcaccggtgg ccgaaacggc accggtgtgg
      121 gcctccagca gtacttcacc ccaccccagg tcggggagct gctggccaaa gtattcaacc
      181 gcgacctgcc cacccttgac ctgaccgccg gcgacggcgg cctgctcgaa tgggtgagcg
      241 acgaacaccg ttacggcatc gaaatcgacc gtgaccaggt aagcaacggc aactacgcca
      301 agcacgcgat cacaggcgac atccagaagg tgtatccgct ccttcgagga ctcggtgcca
      361 cctggccgca actggtggtc aacccgccgt tcgggctcga ctggaaagtg cctggcatag
      421 aagacggcaa gaccggatcg tcaacgcttc gggcattcca gatgatgtgc cggctcaccg
      481 acagctacgg gcaggcagcc ttcatttgcg gccgcgaccg ctacatcaag gaaatcgccg
      541 gccgcccgga agccgaacag gtgtgggcga aaatcgaggt ggatgacctc ttcgacgggg
      601 tggagctacc tgtcgttttg tgcctgaccg ttggcgagct ggttcacaaa accgtggaag
      661 gcaccgttga ccttcacgtg aaaaaggacg agctgaccat gtgctggcca cggctggtcg
      721 cagagcaaga ccagcgcacc gggttccagc ccgttttcgc gaagataggc cagaaccgtt
      781 gcgagtcggt tttcgtgacc gccgccgccg aacacggcag acgcgccaca ggaaccgaac
      841 ggaaaggttt cgacctgtct ttgaccggcc agaaaatccg ggtgcgcctc ggcgagtact
      901 cgaaagtcgc gctcgcacaa ggcggctacc gggaactgga atcggtcagg aacctcaacg
      961 gcctggcaac cagctgggtt gcgctcaacc cgaaaagctg gcgggaaatt atcgggctct
     1021 ccgaccaggg caagatcaca atcgaaccca aactgctcga aaagatcagc gaggtcgaag
     1081 tggaagtgcg ccgtgaactg gtgccgcttt actcggttcg cccgcaacag cggctcgggt
     1141 atctcaccga catagaaaac atcgtgtgcc gtgccgacgc gcccgactac gagttcgtcg
     1201 ccggcgaaag ctacccgatc agcaccagct cgaaaatcga ggaacacgac gaggaaaggc
     1261 cctacgagaa aaaggacgga acccatgaga tccgcaagtt ccgcaccaaa cgaagggtgc
     1321 ttgaaatacg gatcggaaac catgttttct ccgaggccgc agccgacgtc gagtttctgc
     1381 tcgaccattt cgaaatcccg gatccagggg acatagccga ccagcgtcca gacgaggtcg
     1441 ccaaggccct ttcggtgctt gacaacctca ccgaaaccca tgattgggcc gggaaagggc
     1501 ttgactggaa aggccttcac gacccggaaa caggggagtg gatccaatgg caaaaagagg
     1561 atctcgcgcg ggctgtagtc aaaggccggg cgatccttgg ctgggagcag ggtggaggca
     1621 aaagcctcgg tttgctctcg cttgctttgg cagcaatcga atatcaccgg ctgccgcgac
     1681 aagccatgct cgtggtgccg caggaccttc taccccaatg gcgtcgggaa gcaaaacggt
     1741 tcttcgacat tgacttcgaa gtgatcgaca cccccgccaa agcccgccag gtcaagaagc
     1801 gcatgaagcg cggcgaggtg gacatcgagg cagaccagcc agaccatccg ctgtgcgccc
     1861 agctcggcgg catcgagtac aagttcgcct cgggcactgc ggagtcgatg ctcatcctct
     1921 cgaagaagga gctgcgcgag aaggggctca agtccccgga cgcggccgac gcggcgagct
     1981 actcggcggc ggaagtggac gtggagggga tgctcggact ccagcccggg gatggctttg
     2041 cgctcgacat cgacctcctg agcagcagcg tcttctcctc gaacagcttc tggaactagg
     2101 cgaaaagtgg tcgaaaagta acccgaaact aaccccaatc cgcccgaaag tgcggatttg
     2161 ggggg
//
LOCUS       DAOTIP010000367         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_60520_length_2157_cov_3.207422, whole genome shotgun sequence.
ACCESSION   DAOTIP010000367 DAOTIP010000000
VERSION     DAOTIP010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60520_length_2157_cov_3.207422"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="P5138_07395"
     CDS             complement(<1..756)
                     /locus_tag="P5138_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRV60441.1"
                     /translation="MLAETTSFTLDGIAARMIRVEVDVHRGLPKFQIVGLPDAAVREA
                     RERVRAAIANCGFDFPLQRITASLAPADLRKAGPAMDLALAAALLGAAGELCIEPLAG
                     WSLVGELALDGTVRPVRGALAMAEEARGQGRRGVIVPEANGPEAALAEGISVRTIGNF
                     AELAGLDSGEVPGQVAVPAEIRDRDELPDLADLRGQSHLRTAVEVAAAGGHGLLMIGP
                     PGAGKSLAARRMPSVLPPLSGEEILEVARISGAC"
     gene            920..1294
                     /locus_tag="P5138_07400"
     CDS             920..1294
                     /locus_tag="P5138_07400"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRV60442.1"
                     /translation="MDGRRVTGQSAEDICAGRLRERGWRILARNWRIRIGELDLIALS
                     GSTLVIVEVKAHHQGLRRGPEAPALAVGPNKQRRIRRLASAWIAGHGRRVGFREVRFD
                     VVGITFDREGRILSYDHLEDAF"
     gene            1356..>2157
                     /locus_tag="P5138_07405"
     CDS             1356..>2157
                     /locus_tag="P5138_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRV60443.1"
                     /translation="MARSGSLPEVALGNEEAVRAWDTVLYDRWKKNRKVFVGALDEVT
                     DKAFELYPPPEGGSCIDIGCGFGETTVQMAGLVGPEGFALGTDSSPHFIRDAINEAAD
                     SGAGNVAFEVADAQVADWPPAHEYAFSRMGTQFFAAPVPAMRVIRGSLKPEGKLCKIC
                     WQRKEDSALWAETEKIVERFLDHPEESDADTCGPGPFSLGNPETCRGILEAAGFEDVE
                     IEPFEFDYYMGGSMDEAVEAATAIGPGAELIRLNGDFGESRRPEIEAAR"
BASE COUNT          390 a          731 c          713 g          323 t
ORIGIN      
        1 gcaggcgccg gagatcctcg caacttcgag aatctcctcc cccgagagcg gaggcagaac
       61 cgaaggcatc cgccgggcgg caagcgactt accggcaccg ggcgggccga tcatcaggag
      121 gccgtgcccg ccggctgccg ccacttccac cgccgtccgc aggtgggact gtccccggag
      181 gtcggcaagg tccggcaact cgtcccgatc cctgatttcc gccggcacgg ccacctgacc
      241 gggcacctct ccgctgtcga gtccggccag ttctgcgaag ttgccgatcg tgcgcacgga
      301 gatcccctcg gccagtgccg cctcagggcc gttcgcctcg ggaacgatca ccccgcgacg
      361 tccctgcccg cgggcctcct cggccatcgc cagtgcaccg cgcaccggcc tgaccgtgcc
      421 gtcgagggcg agctctccga ccagcgacca gccagccagc ggctcgatgc agagttcgcc
      481 ggcggcgccg agcagcgccg ccgcaagggc gaggtccatc gccgggccag ccttcctgag
      541 atccgccgga gcgaggctgg cggttatccg ctgcagcgga aagtcgaagc cgcagttcgc
      601 tatcgccgcc cgcacccgct cgcgggcctc cctgaccgct gcgtcgggca gcccgacaat
      661 ctggaatttg ggaaggcccc ggtgcacgtc gacttcgacc cggatcatcc gcgcggcaat
      721 gccgtcgaga gtgaacgaag tagtttcggc aagcatgcgg ccaagctagg agcaaagtcc
      781 tcaaacgtgg cttgccgggc gcaacgattc cgcagcagtt cgtgacgcct gccgacgtga
      841 cctcaaggaa ggcgaaaaca tctgtgaaaa ccggccccgg ggtggttgaa aatcgccgct
      901 ccggccctag gttcggagga tggacgggcg acgcgtcacc ggtcagagtg ccgaagatat
      961 ctgtgccggc aggctgcggg agcggggctg gcggatcctc gcccgcaact ggcggatcag
     1021 gatcggcgag ctcgacctga tcgccctgtc gggttcaacc ctggtgatcg tcgaggtgaa
     1081 ggcacaccac cagggactga ggcgtggtcc cgaggcgccg gcgcttgcgg tcggcccgaa
     1141 caagcagcgg cgtatcaggc gcctcgcctc ggcctggata gcgggccacg gtcgccgggt
     1201 cggattccgg gaagtccgct tcgacgtggt cgggatcacc ttcgaccgcg agggacgcat
     1261 cctttcctac gaccacctcg aggatgcctt ctgatcaagc cccgacagag ccggctccaa
     1321 ttgaccagag gaatggcagc aactaggctc gaggcatggc ccgatccggc tcactccccg
     1381 aagttgccct tggaaatgaa gaggcggtcc gcgcctggga cacggtgctc tatgaccgct
     1441 ggaagaagaa ccggaaggta ttcgtcggcg ccctcgacga ggtcaccgac aaggccttcg
     1501 agctttatcc gcctcccgaa ggaggctcct gcattgacat cggctgcggt ttcggcgaga
     1561 cgaccgtgca gatggccggc ctggtcggcc cggaaggctt cgccctcgga accgactcct
     1621 caccccattt cataagagac gcgatcaacg aggctgctga cagcggtgcc ggtaacgttg
     1681 ccttcgaggt cgccgacgcc caggttgcgg actggccacc cgcccacgag tacgccttct
     1741 cccgcatggg aacccagttc ttcgcggcac ctgtcccggc gatgagggtg atcaggggat
     1801 cactgaagcc cgaaggaaaa ctctgcaaga tctgctggca gcggaaggaa gacagcgccc
     1861 tctgggcaga gaccgagaag atcgtcgagc ggttcctcga ccaccccgag gaatccgacg
     1921 ccgacacctg cgggccggga cctttctccc tcggcaatcc ggagacctgc cggggaatcc
     1981 tcgaggccgc ggggttcgag gacgtcgaga tcgagccctt cgagttcgac tactacatgg
     2041 gcggcagcat ggacgaggcg gtcgaagccg ccacggcgat tggaccaggt gccgaactga
     2101 tcaggctgaa cggcgacttc ggggagagcc gacgccccga gatcgaggcc gcccgcg
//
LOCUS       DAOTIP010000368         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_60655_length_2153_cov_3.488561, whole genome shotgun sequence.
ACCESSION   DAOTIP010000368 DAOTIP010000000
VERSION     DAOTIP010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60655_length_2153_cov_3.488561"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="P5138_07410"
     CDS             <1..2079
                     /locus_tag="P5138_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60444.1"
                     /translation="APAGGGREAPSDLAETPDGGYVIAGSATTGSGSIVFRYLPDGSP
                     DPGFGFSGQLRSPGDGWDRVEVLGDGRILLASGASTELKFVRLNADGSPDPSFGGTGT
                     VTLSTSGLFTPPDGVAADASVSSLVILADGSLRAIGTFAGCPSGTYSLCGEAFLAGID
                     SSGNPDTTFGSGGIGNTGIAGRGILSATLPDGRSIVVRSETGDPPYGDVLYSTPVSAD
                     GVTGETKIISRGNSFYWSNLDVYPGGTAVVSGNRIFLVDADHLWKLLPDGDRDPDFGT
                     DGMVELRDLTGFLPDYPGFFATGVDVDGQGRLLVSGAVTSGVGKKEWGGDSWASTGAA
                     ARLNADGSIDPSFGGGGLQILWHGRRANQHLTGNTQVLASGENVVVSGMGPVGISFGF
                     TLARAANDEMEMPTCGGETADYVGFGESDRIQAYDAVVSTLGGTDRIEGGFGSLICSG
                     PGDDVVNVEVGKATVFAGQGNDRISLGNQDSTAYGGAGDDLIRGGPRRDRLFGNDGND
                     LLLGGDRDDRLFGGKGKDRLFGQGGRDWLFGGPGKDLLKVGPIKPDPTDYLPKQPGGF
                     MRVTTLGRKANTTIRISTVCEGKAPSRSYLNMRSIAFDPATGRLSNLDDQQPSLDDDF
                     VFIADIQARVKNGRISGRFRYIEGESYYDNYLCRTGSGPVKEKSALKDAWVPFSGRVE
                     PKPRQFARQN"
BASE COUNT          378 a          680 c          720 g          375 t
ORIGIN      
        1 gcacccgccg gcggcggtcg agaggcacct tccgatctgg ccgaaacccc ggacggtggc
       61 tacgtgattg ccggttctgc caccacgggt tcaggatcga tcgttttcag gtacttgccc
      121 gatggcagcc cggaccccgg tttcgggttc tccggccagc tccgcagccc cggcgacggc
      181 tgggatcggg tcgaggtgct cggcgacggc aggatcctgt tggcgtccgg tgccagcacc
      241 gagctgaaat tcgtccgcct gaacgccgac ggcagcccgg acccgtcctt cggcggaacc
      301 ggaacggtga ccctgagcac cagcggcctt tttactcccc cggatggagt cgcagccgat
      361 gcctccgtct cttctctggt gattctcgct gacggcagtc tgcgcgcgat cggaaccttc
      421 gcgggctgcc cgtccgggac gtacagcctc tgcggcgaag cgttcctggc cgggatcgac
      481 agcagcggca accccgacac aacctttggc agtggcggta tagggaacac cggtatcgcc
      541 ggacgcggca tcctttcggc gactctcccc gacggacggt caattgtcgt ccgaagtgaa
      601 accggcgatc caccgtacgg cgacgtcctg tattcgaccc cggtctcggc cgacggggtg
      661 accggcgaaa cgaagatcat cagccgcggg aacagctttt actggtcgaa cctcgacgtc
      721 taccccggcg gcaccgccgt cgtgtccggg aaccgcatct ttctggttga tgccgatcac
      781 ctctggaagc tgcttcccga cggagaccgg gatcccgact tcgggactga cgggatggtc
      841 gaacttcggg acctgaccgg attcctgccc gactatcccg ggttcttcgc tacaggcgtg
      901 gatgttgacg gccaggggag gcttctcgtc tcgggagcag tcacgagcgg cgtcggcaag
      961 aaggaatggg gcggcgactc ctgggccagc acgggggccg cggccagact caatgcggac
     1021 ggcagcattg acccctcgtt cggcggggga ggccttcaga tcctctggca cgggaggcgg
     1081 gccaatcagc atctgaccgg gaacacgcag gttctggctt ccggcgagaa cgtagtcgtc
     1141 tccgggatgg ggccggtcgg catcagcttc ggcttcacgc tggcccgcgc ggccaacgac
     1201 gaaatggaga tgccgacctg cggcggggag acggctgact acgtcggctt cggggaaagc
     1261 gaccggattc aggcctatga cgccgtggtc tccacactcg gcggaactga ccggatcgaa
     1321 ggcggtttcg gatccctgat ctgctcgggc cccggtgacg atgtggtgaa cgtcgaagtc
     1381 gggaaggcaa ccgtgttcgc cgggcaggga aatgaccgca tcagtctcgg caatcaggac
     1441 agcacggctt atggaggcgc cggcgacgac ttgatccgcg ggggtcccag gcgagacagg
     1501 ctcttcggca atgacgggaa tgatctgctc ctcggcggcg accgggatga ccgcctgttc
     1561 ggaggcaagg ggaaggaccg gttgttcggc caagggggcc gcgactggct gttcggcggg
     1621 cccggcaaag acctcctgaa ggtgggtccg atcaaacccg atccgaccga ctacctgccg
     1681 aaacagcccg gcggcttcat gcgggtcacc actctcggtc gcaaggccaa caccacgatc
     1741 cggatttcga cggtctgcga gggcaaggcc ccttcgcgtt cctacctgaa catgaggagc
     1801 atcgccttcg atcccgccac cggcaggttg tccaatctgg acgatcagca gccgtcgctc
     1861 gatgacgatt ttgttttcat cgccgacatc caggcccggg tgaagaacgg ccggatctcc
     1921 ggacggttcc ggtacatcga aggggagtcc tactacgaca actacctgtg ccggaccgga
     1981 tcagggccgg tgaaggaaaa gagcgctctc aaggatgcct gggtgccatt ttcgggaagg
     2041 gttgaaccca agccccgcca gttcgccagg cagaactagc gggcttcagg cgctgacggt
     2101 ttcggcttcg gcgggctcgg cgtccagcca gcggattcca tactggcgca tgt
//
LOCUS       DAOTIP010000369         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_60925_length_2146_cov_3.483979, whole genome shotgun sequence.
ACCESSION   DAOTIP010000369 DAOTIP010000000
VERSION     DAOTIP010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60925_length_2146_cov_3.483979"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="P5138_07415"
     CDS             complement(<1..961)
                     /locus_tag="P5138_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV60445.1"
                     /translation="MEANTMSTGSAPSTPAAGGGISSSYDAIVVGGGHNGLTTAAYLA
                     RAGMKTLVLERRPILGGACVTEEVWPGARVSRCSYVVSMLQSKVVSDLRLKDFGYKAY
                     PLDPAYAAMTEDGPIFFFNEVERTVESIRKFSPKDADNYEAFEDLLERTASFLRPMLL
                     REPPALGSKAPGDIASLIREGARIAGLTRRDVNDLIRIFTMSVGDLLDDWFELDALKG
                     SIASTGVVGVWAGPRTPGTAYNLLHHALGELDGMGGVWGQVVGGMGAISNAIARSAES
                     HGATIVTDAEVASIDVEGGRVVGVTLANGDAIRAPIVASGAHPK"
     gene            1067..>2146
                     /locus_tag="P5138_07420"
     CDS             1067..>2146
                     /locus_tag="P5138_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV60446.1"
                     /translation="MSGESSTADFTSAVNLETSAKRHLMLHFSDMKSLVEEGITIIER
                     GEGVHVFDMQGDKYIDGLSGLYCASLGHSYGDEIGEVAHEQWKKLPYATNWTVAHPPS
                     IELAEKIASLAPEGMNKVYFTSGGSESVEAAWKMAVQWQQANGHERRRKVIARRDAYH
                     GVTMGALSFTGIPVCRTPFDPLGIPTVHVSNTSSYRHPEGEDEAAFCQALLDEVEETI
                     EFEDPSTIAMMIAEPVQNAGGCFMPPAGYWQGLRKICDKHDILLCSDEVICAYGRIGT
                     WFGGQKFDYVPDLVTFAKGLTGAHFSMGGVLMSDKVAEPFFDGRADYNNGYTFGGHPV
                     GAAVALKSLEILEREKVLENVNSNAP"
BASE COUNT          390 a          675 c          724 g          357 t
ORIGIN      
        1 tcttcgggtg ggcgccggag gcgacgatcg gggcgcggat cgcgtcaccg ttggcaagcg
       61 tcacgccgac cacccggccg ccttcgacgt cgatcgacgc gacttcggcg tcggtcacga
      121 tcgtcgcgcc atgtgattcg gccgaccggg cgatcgcgtt cgagatcgcg cccatcccac
      181 cgaccacctg gccccagacg ccacccatgc cgtcgagttc gccgagtgcg tggtggagca
      241 ggttgtaggc ggtgccggga gtgcgggggc cggcccagac tccgaccacg ccggtcgagg
      301 cgatcgaccc cttcagggcg tcgagctcaa accagtcgtc gagcaggtcg ccgaccgaca
      361 tggtgaagat gcggatcagg tcgttgacgt cgcgtctggt cagtccggcg atgcgggcgc
      421 cctcgcggat cagggaggcg atgtcaccgg gtgccttcga gccgagggcg ggcggttcgc
      481 gcagcagcat cgggcgcagg aacgaggcgg tgcgttcgag caggtcctcg aaggcttcgt
      541 agttgtcggc gtccttaggg gagaacttgc ggatcgactc gaccgtgcgc tcgacctcgt
      601 tgaagaagaa gatcgggccg tcctcggtca tcgccgcgta ggcgggatcg agcgggtagg
      661 ccttgtagcc gaagtccttg aggcggaggt cggagacgac cttggactgg agcattgaga
      721 ccacatagga gcagcgcgaa acccgcgctc ccggccagac ctcctcggtc acgcaggcgc
      781 cgccgaggat cggccggcgt tccagaacca gggtcttcat gccggcccgg gccaggtacg
      841 cggccgtggt caggccgttg tggccgccgc cgaccacgat cgcgtcgtag ctcgaggaaa
      901 taccgccgcc tgccgccggg gtcgaagggg cactgccagt ggacatcgtg ttcgcctcca
      961 tgagccgaca ctaccgtaaa gtgcgattga ctacaaggtc aatactcgct ataggactat
     1021 aagttcggtc gcgggcggat cggagccggg ggatagactc ggtcacatga gcggagagag
     1081 cagtacggct gatttcacct cggcggtgaa cctggaaacc tcggcgaaac ggcatctgat
     1141 gctgcatttc tccgacatga agtccctggt cgaggaaggc ataacgatca tcgagcgagg
     1201 cgagggcgtc cacgtcttcg acatgcaggg cgacaagtac atcgacgggc tgtccgggct
     1261 ctactgcgct tcgctcggcc attcctacgg tgacgagatc ggcgaagtcg cccatgagca
     1321 gtggaagaaa ctgccctacg ccaccaactg gaccgtggcc catcctcctt cgatcgaact
     1381 ggccgagaag atcgcctcgc tcgccccgga agggatgaac aaggtctact tcacctcggg
     1441 cggttcggag tcggtcgaag ctgcctggaa gatggcagtc cagtggcagc aggccaacgg
     1501 acatgaacgg cggcgcaagg tgatcgcccg ccgggacgcc taccacgggg taaccatggg
     1561 agcgctctcc ttcaccggaa tcccggtctg ccgcacaccc ttcgatccgc ttggtatccc
     1621 gaccgtccac gtcagcaaca ccagctccta ccggcatccg gagggcgagg atgaggccgc
     1681 cttctgccag gccctgctcg acgaggtcga ggagacgatc gagttcgagg acccctcgac
     1741 catcgcgatg atgatcgccg agccggtcca gaacgcgggc ggctgcttca tgccgccggc
     1801 cggctactgg caggggctga ggaagatctg cgacaagcac gacatcctgc tctgctctga
     1861 cgaggtgatc tgcgcctacg gccggatcgg cacctggttc ggtggccaga agttcgacta
     1921 cgttccggac cttgtcacct tcgccaaggg cctgaccggc gcccacttct cgatgggcgg
     1981 ggtgctgatg agcgacaagg tcgccgagcc gttcttcgac ggtcgggccg attacaacaa
     2041 cggctacacc ttcggtggcc atccggtcgg cgctgccgta gccctcaagt cgctggagat
     2101 ccttgagcgc gagaaggtgc tcgagaacgt gaactcgaat gcgccg
//
LOCUS       DAOTIP010000370         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61043_length_2143_cov_3.222222, whole genome shotgun sequence.
ACCESSION   DAOTIP010000370 DAOTIP010000000
VERSION     DAOTIP010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61043_length_2143_cov_3.222222"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="P5138_07425"
     CDS             <1..846
                     /locus_tag="P5138_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60447.1"
                     /translation="QKAGSAEPNAWLGFNDPAAGDMHSFDVSSNGITVLEVTPEGSDA
                     SEFSFWNTNGTPPAGDPSYRCSVPGIAQAPAYPRFSPDGSMITWQDRGSVYVAHVPAS
                     TGGGTCTLNPVKVGNGKEPAFGLVTLQPTGPTGPTGPTNPTGPTGPTNPTGPTGPTSP
                     TGPTGPTGPTGPSQPKVALKVNPGTAKVKAGRKVSLTAAVTASGGTASTVKVCATVTG
                     KVRKAIRPAACRTLRNVAAGRTVKTGLALRASRQARGIYPIRITASGPGFSPVKSTNK
                     VKVTK"
     gene            843..1373
                     /locus_tag="P5138_07430"
     CDS             843..1373
                     /locus_tag="P5138_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60448.1"
                     /translation="MNTRNRNNPEMINRPVSGTRLADGGFRTALALFTCLFLAWSLFA
                     ADFAGAAPYKGKTKGGSSITFALKGKKVTGIRTVVPTLCLETTGGYASRAGSELFQPT
                     AAARVGRTVKSKALQPAAMNVGAKATKNYEVSLKQRGKTVSGKLKLNFSFLIPDLYGA
                     HIYICSGTTTFSARPR"
     gene            1432..1824
                     /gene="mscL"
                     /locus_tag="P5138_07435"
     CDS             1432..1824
                     /gene="mscL"
                     /locus_tag="P5138_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRV60449.1"
                     /translation="MIQGYREFITRGNVIDLAVAVVIGAAFTGLVNSVVEDLLTPIIA
                     AIIGEPDFSALSFTVNGSVFTYGNFINALITFLSISAVIYFFVVLPMNHLKRDEEATH
                     RDCPECLSEIPVKATRCAHCTAELTPAA"
     gene            complement(1846..>2143)
                     /locus_tag="P5138_07440"
     CDS             complement(1846..>2143)
                     /locus_tag="P5138_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60450.1"
                     /translation="GSRLPLHYHQGTQVGYIQSGVLTYHVQSGRARLMRGAGDAPKLI
                     RVIRAGQTVRVRPGQWLIEQPYDHHRAANYGRKPVVVLLANLLLKNAAPSKLIP"
BASE COUNT          379 a          774 c          649 g          341 t
ORIGIN      
        1 cagaaggccg gcagcgccga gccgaacgcc tggctcggtt tcaatgaccc ggcggcggga
       61 gacatgcaca gcttcgatgt ctcgtcgaac gggatcacgg tgctggaagt caccccggaa
      121 ggatcggacg cctccgagtt ctcgttctgg aatacgaacg ggaccccgcc cgccggtgat
      181 ccctcttacc gctgctcggt ccccgggatc gcccaggcac cggcctaccc gcgcttctcg
      241 cctgacggtt cgatgatcac ctggcaggac cggggatcgg tctacgtggc ccacgtcccg
      301 gccagcaccg gtggaggcac ctgcaccctg aacccggtca aggttggcaa cggcaaggag
      361 cccgcctttg gcctggtgac gctccagccg accggaccca cggggccgac cggaccgacc
      421 aatccgaccg gtcccaccgg tccgacgaac ccgaccgggc ccaccggacc caccagtccc
      481 accggaccga ccggaccgac cggacccact ggtccctccc agccgaaggt cgccctcaag
      541 gtgaacccgg gcacggcaaa ggtcaaggcc ggccgcaagg tttccctgac cgcggcggta
      601 accgcttccg gcggcacggc gagcacggtc aaggtctgcg cgaccgtcac gggcaaggtc
      661 aggaaggcga tcaggccggc tgcctgcagg accctgcgca atgtcgctgc cgggcgaacc
      721 gtgaagaccg gactggccct ccgcgccagc cgtcaggccc ggggcatcta cccgatccgc
      781 atcaccgctt ccggcccggg cttctcccca gtcaaatcca ccaacaaggt caaggtgacc
      841 aagtgaacac cagaaatcga aacaaccccg aaatgatcaa tcgtcccgta agtggcaccc
      901 ggctggccga cggtggcttc cgaacagccc tcgccctgtt cacctgcctc tttctggcct
      961 ggtcgctgtt cgccgcggac ttcgccggcg ccgcgcctta caagggcaag accaagggcg
     1021 gaagctcgat caccttcgcg ctcaagggca agaaggtcac cggcatccgc accgtcgtcc
     1081 cgaccctctg cctcgagacg accggtggct acgcctcccg ggccggcagc gaactgttcc
     1141 agccgacggc agccgccagg gttggccgga ccgtcaagag caaggccctg cagccggccg
     1201 cgatgaacgt cggcgcgaag gcgaccaaga actacgaggt cagcctgaaa cagcgcggaa
     1261 agacggtatc gggcaagttg aagctcaact tctccttcct gattccggat ctctacggag
     1321 cccacatcta catctgctcc ggcaccacca cgttcagcgc caggccccgc tgatttctcc
     1381 ggcggccccg atttcccgtc ggggtcgccg ggtaatttcc ggcccgtgaa gatgattcag
     1441 ggctaccggg agttcatcac ccgcggcaac gtgatcgacc tggccgtggc ggtcgtgatc
     1501 ggagccgcgt tcacgggcct ggtcaattcg gtcgtcgagg acctgctcac cccgatcatc
     1561 gccgcgatca tcggtgagcc cgatttctcg gccctcagct tcacggtcaa cgggtccgtg
     1621 ttcacctacg gcaacttcat caacgccctg atcactttcc tctcgatctc ggcggttatc
     1681 tacttcttcg tggtcctgcc gatgaaccac ctcaagcgcg acgaggaagc cacccaccgc
     1741 gattgtcccg aatgcctttc cgagatcccg gtcaaggcga cccgctgcgc gcactgcacc
     1801 gccgagctaa ccccggccgc ctgagcgccg cccttgcgtc cggcgctagg ggatcagctt
     1861 cgaaggtgcc gcgttcttga ggaggaggtt ggcgagcagg accactacgg gcttgcggcc
     1921 gtagttggcc gcccggtgat ggtcgtaggg ctgctcgatc agccactggc ccggcctcac
     1981 cctcacagtc tggccggccc tgatcacccg gatcagcttc ggggcatcac ccgcgcccct
     2041 catcagccgg gcccgcccgg actgcacgtg gtaggtcagg actccggact ggatgtagcc
     2101 gacctgcgtg ccctggtgat agtggagggg aagtctggac ccc
//
LOCUS       DAOTIP010000371         2141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61108_length_2141_cov_3.426174, whole genome shotgun sequence.
ACCESSION   DAOTIP010000371 DAOTIP010000000
VERSION     DAOTIP010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61108_length_2141_cov_3.426174"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="P5138_07445"
     CDS             <1..690
                     /locus_tag="P5138_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60451.1"
                     /translation="HAEWCRNEIARLEGGETREAELREEVEAAGQELEKAAGSLSRAR
                     RKAASELATRVSKDLEDLAMPGAELTIDLNPVAEGPGASGSETAEFMLAPNPGMPAQP
                     LRDTASGGELSRVMLALAAGGAGDRRTMIFDEIDAGIGGNTAGVVGERLRDVSDGRQV
                     LAITHLPQVASRAVRHFSIEKETGKDAVSATVTALEGDLVVEEIRRMLGAAEGDEAAT
                     RHARELVSANQ"
     gene            725..1918
                     /gene="steA"
                     /locus_tag="P5138_07450"
     CDS             725..1918
                     /gene="steA"
                     /locus_tag="P5138_07450"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HRV60452.1"
                     /translation="MNTITRRLRGRPASGMPEPNGGRVVASRGRIRLGHKTKDLVKTI
                     GPGDVAVISHCNIDRIAAEDLVASGVKVVLNNDASSDGRYPNRGPLILVGAGVRLIDF
                     PDVDLFDLLVENEHVLVEGGEIKRDGTTVATGVEQLEPDLERQFSTQQAEIHHALADF
                     AENTVTHIQEESELLSGAIELPDTRTDFRERHVLIVVRGPNYKRDLRALRAYIRDVKP
                     ILVGVDGGADAIREAGYSPDMILGDMDSVTDATLKSGPEVVVHAYADGRAPGVERMVE
                     LGVEHIAVPFVGTSQDVAMLMAYEKGASLIVTVGAHFNLIEFLDKDRAGMSSTFLTRL
                     RIGEVLVDAKGVSRLYNPGLTVGPLMLFLGAFLVLATIVVLSSPALADLFDLIWLKIK
                     IWLGL"
     gene            1929..>2141
                     /locus_tag="P5138_07455"
     CDS             1929..>2141
                     /locus_tag="P5138_07455"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HRV60453.1"
                     /translation="MGYSARYHAASLAAVFLALAIGILIGSQWGSDVLNKTRKDLESS
                     LTSDLNDARANIDDLNRQLDQSNQFGG"
BASE COUNT          401 a          668 c          735 g          337 t
ORIGIN      
        1 cacgcggaat ggtgccggaa cgagatcgcc cggctggaag gcggggaaac ccgcgaagca
       61 gaactccggg aagaagtgga agctgccggg caggaactcg agaaggccgc gggatcgctg
      121 agtcgggccc gccggaaggc cgcctccgaa ctcgccacgc gagtcagcaa ggacctggag
      181 gatctggcca tgccgggagc cgagctgacg attgacctca acccggttgc ggaagggccg
      241 ggagcatccg gatcggagac ggccgagttc atgctggcgc cgaatccggg aatgccggcg
      301 cagccactcc gggacaccgc atcggggggc gagctctcgc gggtcatgct cgcgcttgcc
      361 gccggcgggg cgggagacag gcgcacaatg atctttgacg agatcgacgc aggcatcggc
      421 ggaaacacgg ccggagtcgt cggggaacgc ctgcgggatg tatccgatgg ccgtcaggtg
      481 ctcgcgataa cccacttgcc acaggtagcc agccgggccg tgaggcattt cagcatcgag
      541 aaggagaccg gaaaggacgc ggtttcggcg acagtgacgg cgcttgaggg ggatctggtg
      601 gtggaagaga tccggcgcat gctcggggcc gccgaaggcg acgaggcggc gacccgccat
      661 gcacgggaac tggtgtccgc caaccaatag ctttttcgcc gcccggttgc cctaccttta
      721 aggggtgaac acgatcacga gaaggctgcg cgggcggccc gcttccggca tgccggagcc
      781 gaatggcggc cgggtcgtgg ccagtcgggg gcggatccgg ctggggcaca agaccaagga
      841 cctggtcaag acgatcgggc ccggcgacgt tgcagtcatc tcgcattgca acatcgaccg
      901 gatcgccgcc gaggacctgg ttgccagcgg ggtcaaggtc gttctcaaca acgacgcttc
      961 ctccgacggc cgctatccca accgcgggcc gctgattctg gtcggggccg gggtgaggct
     1021 gatcgacttc ccggacgtgg atctcttcga ccttcttgtc gaaaacgaac atgtcctggt
     1081 cgagggcggc gagatcaagc gggacggaac gaccgtggcc accggcgtgg agcagctcga
     1141 gcccgacctc gagcggcaat tctcgacaca gcaggccgag attcaccacg cgctcgccga
     1201 tttcgccgag aacaccgtca cccacatcca ggaagagagc gagcttcttt ccggtgcgat
     1261 cgagttgcct gacacccgca ccgatttcag ggagcgccat gtcctgatcg tggtgagggg
     1321 ccccaactac aagcgggacc tgcgagcctt gcgggcctac atccgggacg tgaagccgat
     1381 cctggtcggc gtcgacggcg gggccgacgc cattcgcgag gccgggtaca gcccggacat
     1441 gatcctcggg gacatggact cggtcaccga cgcgaccctc aagtcaggcc cggaagtggt
     1501 cgttcacgcg tatgccgacg gccgggcgcc cggggtcgag cggatggtgg aactgggggt
     1561 tgaacacatc gccgttccct tcgtcgggac cagccaggac gtggcgatgt tgatggccta
     1621 cgaaaagggc gcctcactga tcgtcacagt cggcgcccac ttcaacctga tcgagtttct
     1681 cgacaaggac cgggcgggaa tgtcctcgac cttcctgacc aggctcagga tcggagaggt
     1741 cctggttgac gcgaaagggg tgagcaggct ctacaacccg ggcctgaccg tcggcccgct
     1801 gatgctcttc ctcggggcct ttctggtgct ggccacgatc gtggtactca gctcgcccgc
     1861 cctggccgac ctgttcgatc tgatctggct caagatcaag atctggctgg gcctctaggc
     1921 ttcccgcgat gggttacagc gcgcgctacc acgcggcctc gctcgcggca gtctttctcg
     1981 ccctggcaat cggcatcctg atcggttccc agtggggcag cgacgtgctg aacaagaccc
     2041 gcaaggacct cgagtcaagc ctgaccagcg atctcaacga cgcccgggcc aacatcgatg
     2101 acctcaaccg gcagctggat cagtcgaacc agtttggagg c
//
LOCUS       DAOTIP010000372         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61143_length_2140_cov_3.541487, whole genome shotgun sequence.
ACCESSION   DAOTIP010000372 DAOTIP010000000
VERSION     DAOTIP010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61143_length_2140_cov_3.541487"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="P5138_07460"
     CDS             complement(<1..296)
                     /locus_tag="P5138_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRV60454.1"
                     /translation="MNGADGSRVDRLVAALATAGPDASPLDAYLVTNLVNVEYLTGFT
                     GTNGACLVSNERRVFLTDFRYAERAAAIQGWETEIISGLWLEGLARRLEGRTGF"
     gene            complement(293..742)
                     /locus_tag="P5138_07465"
     CDS             complement(293..742)
                     /locus_tag="P5138_07465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRV60455.1"
                     /translation="MSAATNRVAVLHGVNFDVLEKRDPDLYGGLSLAELEYRIGGWAG
                     EFGLEPIFFQSNSEADFCGYLHRVSETADGAIVNAGAWSHYSRAIGDALAVDPVPTVE
                     VHLSDVENREDWRKVSVFEGLVEAKICGKGVDGYREALELLAGRLGV"
     gene            complement(739..>2140)
                     /gene="aroB"
                     /locus_tag="P5138_07470"
     CDS             complement(739..>2140)
                     /gene="aroB"
                     /locus_tag="P5138_07470"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRV60456.1"
                     /translation="EAAFRDLEREVVLGALADPSIDVLALGGGAVESEAIREALMVDG
                     HTVVWLEISADAAWRRVRKSKRPLARDESAFRALFKARQSLYGSVADAILPGVARRIW
                     DRAYGAVRGLEELPEGTRMFWAVSSGGEYPVYVGRELLGSALFGEGSAAMPGSGRSFC
                     FTDEAVGPIYADRLGEVTATFTVPPGETAKSMAQFDESLREMARAGVTRSDRLIALGG
                     GVVGDLGGFAAHSYQRGIPVVQVPTTLVAQVDSAYGGKTGVDLPEGKNYVGAFHLPSA
                     VVSDVDTLATLPEPELTAGMAEVVKTALLAGGGLWEEVAALQPGEILARADLVFACAR
                     YKCAVVAADERDTGLRAQLNLGHTVGHAIESVTSYERYRHGEAIALGLLAALRLSGAE
                     SLREQVREWNLRHGLPVELDPSIDPGSVVEAVQFDKKKTSRGVGFVLLNEPGKPMIDR
                     PVEDAAILASVEELLP"
BASE COUNT          352 a          753 c          697 g          338 t
ORIGIN      
        1 tcgaatccgg tccggccttc gagacgacgg gccagcccct ccagccacag cccggagatg
       61 atctccgtct cccagccctg aatcgccgcc gccctctccg cgtaccggaa gtcggtcagg
      121 aagacgcggc gttcattcga gacgaggcag gccccgttgg tcccggtgaa accggtcagg
      181 tactcgacgt tgaccagatt ggtgacgaga taggcgtcca ggggagaagc atccggcccc
      241 gcggtcgcga gcgctgcgac cagccggtcg actctggagc catcggcccc gttcataccc
      301 cgagtcgccc cgcgaggagt tccagcgcct cgcggtaccc gtctactccc ttgccgcaga
      361 tctttgcctc aaccaggccc tcgaacacgg aaaccttgcg ccagtcttca cgattctcga
      421 cgtcggagag atgaacctcc acggtgggca ccggatcgac cgcgagggca tccccgatcg
      481 cccggctgta gtggctccag gcgccggcgt tgacgatcgc tccgtcagcg gtctcggaca
      541 cgcgatgcag gtacccgcag aaatccgctt ctgaattgga ctggaagaag atcggctcga
      601 ggccgaactc gccggcccag ccgccgatcc tgtattcgag ctcggcgaga ctgaggccgc
      661 catagaggtc agggtcgcgc ttttccagca cgtcgaagtt gacgccgtgg aggacggcca
      721 cccggttggt cgcggcgctc acgggagcag ctcctcgacc gaggcgagaa tcgccgcgtc
      781 ttcgacgggc cggtcgatca ttggtttgcc cggttcgttc aagagcacga agcctacccc
      841 gcgggaggtt ttcttcttgt cgaactggac ggcttccacc accgagcccg ggtcgatcga
      901 cggatccagt tcgaccggga gaccgtggcg gaggttccat tcgcgaacct gctcccgaag
      961 ggattccgcc ccggaaaggc gaagagccgc cagcagcccg agggcgatcg cctcgccgtg
     1021 acggtatctc tcgtaggagg tgaccgactc gatcgcgtga cccacggtgt ggcccaggtt
     1081 gagctgggcc cgcagcccgg tgtcacgctc gtcggccgcc acgaccgcgc atttatagcg
     1141 ggcgcaggcg aagacgaggt cggcgcgggc gaggatctcg ccgggctgga gtgccgcgac
     1201 ttcctcccag agaccgccgc ccgcgagcaa cgccgtcttc acgacttccg ccatcccggc
     1261 ggtcagttcg ggctccggca gggtggcaag ggtgtcgacg tcgctgacca cggccgaggg
     1321 caggtgaaat gcgccaacgt agttcttgcc ttcgggcagg tccaccccgg tcttgccgcc
     1381 gtaggcggaa tcgacctggg ccaccagtgt ggtcggaacc tggaccaccg gtatgccccg
     1441 ctggtagctg tgagcggcga atccgccgag gtccccgacc accccgcctc cgagggcgat
     1501 caatcggtcc gacctggtga cgccggcccg ggccatctcc cgcagcgact cgtcgaactg
     1561 cgccatcgac ttggcggtct cgcccggcgg gaccgtgaag gtcgcggtga cttcgccgag
     1621 ccggtcggca tatatcggcc cgaccgcctc atccgtgaag cagaacgaac gcccgctgcc
     1681 gggcatggcc gccgatcctt cgccgaacag cgctgatccg agaagttccc ttcccacgta
     1741 gaccgggtac tcgccgccgg agctcacggc ccagaacatc cgggttcctt cgggcagctc
     1801 ttcgagaccg cgcaccgccc cgtaggcacg gtcccagatc cttctcgcca ctccggggag
     1861 gatcgcgtcg gcaaccgacc cgtaaagcga ctggcgggcc ttgaacaggg cccggaaggc
     1921 cgactcgtcg cgggccagcg ggcgtttcga tttcctcacc cggcgccagg ccgcgtcggc
     1981 gctgatttcc agccagacga ccgtgtggcc gtcgaccatc agcgcctcgc ggatcgcctc
     2041 ggattcgacg gctcccccgc ccagcgcgag gacgtcgatc gaagggtcgg ccagggcgcc
     2101 gaggaccacc tcgcgctcga ggtcacggaa ggcggcttcg
//
LOCUS       DAOTIP010000373         2139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61179_length_2139_cov_3.707774, whole genome shotgun sequence.
ACCESSION   DAOTIP010000373 DAOTIP010000000
VERSION     DAOTIP010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61179_length_2139_cov_3.707774"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..296)
                     /locus_tag="P5138_07475"
     tRNA            complement(224..296)
                     /locus_tag="P5138_07475"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:His,seq:gtg)
     gene            382..1032
                     /locus_tag="P5138_07480"
     CDS             382..1032
                     /locus_tag="P5138_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HRV60457.1"
                     /translation="MRREMRGFVIGSLLFALGATPGYLGLVGTTADNLTYFLGSIFFT
                     IAGFTQLRLTGRWQPGGWSSRDDWDDWWAAAIQFLGTIAFNISTFVAIAQNLDSETYQ
                     HHVWRPDFLGSIFFLVASALAVKATTHRSSLWDPEARNWWSTWLNMIGSVAFMASAIA
                     AWSDPSTGQQLSTYWVNAGTFIGAICFMSAAILLRPEARRESDPSLNGNGPHPEED"
     gene            complement(1051..1935)
                     /locus_tag="P5138_07485"
     CDS             complement(1051..1935)
                     /locus_tag="P5138_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV60458.1"
                     /translation="MPSSTSAPVPGSPTVPAVVMVIGAISSIQIGAAIATNLFDEVGA
                     SGAVFLRLFISAVLLAVIWRPAYRMDRRSQKLALAFGGVMAGMNICFYQAIDRIPLGT
                     AVTFEFIGPLLVALITSHRRRDLVWAAMAAAGILLLSGGIESHGLDPWGVALAIVAGL
                     FWGGYILLGKRVGETWTGGKGLAVAMAVSALIAAPFGVADGGAKLLDPAVLAIGAAVA
                     ILSSTFPLSLEMEAMRRLASNVFGVMMSLEPAIAATVGFVLLSQGMNPTQLVAIALVV
                     SASAGALWSSRAPAPVEP"
BASE COUNT          441 a          724 c          645 g          329 t
ORIGIN      
        1 ccacgccccg gggtgttgac ccaccgccgg ggcacctaca tattcgggac cgaacctagc
       61 tccaaaccgg accgaaagcg agccgaaacc agcgcaatcg acgccgatcg acgcatatca
      121 acccgaactt caggcactta gtgcctgaac tgccagggaa acgcgggcgg gttcaaacgc
      181 acttgcaaag cggaaacccc attgcgatgg ggtttccgag aggggtgagc gacggggatc
      241 gaacccgcga ccgcctggac cacaaccagg agctctacca actgagctac gcccaccgcg
      301 agacgaggaa atctaccggt cgtgggtcgg atacgctgcg ccggtgccgg aacggatgaa
      361 tcagggggaa agggcttgag cgtgcgccgc gagatgcgcg gcttcgtgat cggctccctg
      421 ctgttcgcgc tcggcgcgac cccgggttac ctgggtctgg tcggcaccac ggcggacaat
      481 ctcacctact tcctgggatc gatcttcttc accatcgccg gcttcaccca gctccggctg
      541 accggaaggt ggcagccggg aggatggtcc tccagggatg actgggacga ctggtgggcg
      601 gccgcgatcc agttcctcgg gacgatcgcc ttcaacatct cgactttcgt cgccatcgcc
      661 cagaacctcg attccgagac ctaccagcat cacgtctggc ggcctgattt cctcggatcg
      721 attttctttc tcgtcgcttc ggcgctggct gtcaaggcga cgacccatcg cagttctctc
      781 tgggacccgg aggcacgcaa ctggtggagc acatggctca acatgatcgg gtcggtcgcc
      841 ttcatggcct cggctatcgc cgcctggtcg gatccctcga ccggccagca gctgagcacc
      901 tactgggtca acgccgggac cttcatcggg gcgatctgct tcatgtcggc cgcaatcctc
      961 ctgaggccgg aagcaagacg ggagtcagat ccatccctca acgggaacgg acctcatccc
     1021 gaggaggact gagggcaccc gacggacggg tcagggctcg accggagccg gtgcccggct
     1081 cgaccagagc gccccggccg aggccgagac gaccagggcg atcgcaacca gctgtgtcgg
     1141 gttcattccc tgcgagagca gcacgaaccc gacggtcgcg gcaatcgccg gttcgaggct
     1201 catcatcacc ccgaagacat tcgaggcgag ccggcgcatt gcttccatct ccagcgaaag
     1261 agggaacgtc gaactgagaa tcgccaccgc cgcaccgatg gccagaactg ctggatccag
     1321 cagcttcgcg ccgccatcgg ccacgccgaa cggagcagcg atcagagcgg agaccgccat
     1381 cgcgaccgcc agacccttac cgcccgtcca ggtctcgccg acccgcttgc cgagaaggat
     1441 gtagccaccc cagaacaatc cggccacgat ggccaatgcc actccccagg gatccagtcc
     1501 gtgactctcg ataccgccgg agagcagcag gattccagcc gcggccatcg ccgcccagac
     1561 caggtcacgc cgtcgatgtg aagtgatcag agcgaccagc agcggaccga tgaactcgaa
     1621 ggtgactgcg gtgcccaacg ggatccggtc gatcgcctgg tagaagcaga tgttcatgcc
     1681 ggccattacc ccgccgaagg ccagggccag tttctgcgac cgtcggtcca tccggtacgc
     1741 ggggcgccag atcaccgcca gcagaaccgc tgaaatgaaa agacgcagga acactgcgcc
     1801 cgaggcaccc acctcgtcga agaggttcgt cgcgatcgcc gccccgatct gaatggaact
     1861 gatcgcaccg atcaccatca ccaccgccgg aacggtgggc gatccgggaa ccggagctga
     1921 ggtggacgag ggcatccggg cagtgaatca accgtagaga ttccgtgcca cgaagccggc
     1981 aaggtccgcg acactccgcc cgattgtcgc ttcgggataa tcaccgcatg agcgactcgc
     2041 caccttcccc tgacgggtac ccgcacttcc tcaccattcc gacccgctgg aaggacaacg
     2101 acatctacgg gcacgtgaac aacgtcgagt actactcgt
//
LOCUS       DAOTIP010000374         2133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61434_length_2133_cov_2.729066, whole genome shotgun sequence.
ACCESSION   DAOTIP010000374 DAOTIP010000000
VERSION     DAOTIP010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61434_length_2133_cov_2.729066"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="P5138_07490"
     CDS             <1..806
                     /locus_tag="P5138_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV60459.1"
                     /translation="LWDLAFGGEITNDSFAPLRAPRLRSVPTYEPGGRRFSRRRAATG
                     PTVQGRWSLTETLFRNAPAAGPRLRAQAELMLERYGILTREMALAEGIPGGFSTLYPE
                     LSNLEVLGTARRGYFVEGLGGAQFALSGAVERLRALPAEENGPDTFTILAATDPASPW
                     GSTLPWPKLESGRKAARAAGAYVLARNGHPLLYVERGGKGLLRLDPSLEGEALAAALS
                     ALTEEANAGRLGQLKIERFDGEPILGSTFEQLLVAAGFSRQPRRLVAPG"
     gene            889..1578
                     /locus_tag="P5138_07495"
     CDS             889..1578
                     /locus_tag="P5138_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRV60460.1"
                     /translation="MTSAEESTVGLLRSVPLFAELDPDELEQFSRVAIPRSYPAGTRV
                     FHEGDHSDACYIVKSGTFRVTREHPDGRAITLATLGEGDIVGELAMLDGEVRSASVET
                     LGGDGELLALPANDVRSLLERHPEITVKFVAALTRRLRSANERISRQSFQTVPSRVAG
                     VLSQMVAEEGEDGRQVTIRMNQADLAQLAGTSRESVSRFLADLERSGVVKAGRGQVTI
                     LEPARLGNYIF"
     gene            1588..1983
                     /locus_tag="P5138_07500"
     CDS             1588..1983
                     /locus_tag="P5138_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HRV60461.1"
                     /translation="MTGLLALNFYSPLFVDQLRRGRKTATIRLGNKSGKYQRSQIVWV
                     TVGSRHSRREKIFSAVIDEVEVKRLSELSPRDIERDNPEFRRVDETRNFLQKIYDRPI
                     EEDAIVTVVHFSQIVEPGLTGYGTVPSPN"
BASE COUNT          314 a          761 c          684 g          374 t
ORIGIN      
        1 ccctctggga cctcgccttc gggggtgaga tcaccaatga ttcattcgct cccctcaggg
       61 ccccgcgtct gcgttcggtc ccgacctacg agcccggcgg tcgccgcttt tcgcgtcgcc
      121 gggccgcgac cggtccgacc gtccagggcc gctggtcgct gaccgagacc cttttccgga
      181 acgcaccggc ggccggcccg aggctgcgag cccaggccga actcatgctc gagcgctacg
      241 ggatcctgac ccgggagatg gcgcttgccg aaggaattcc gggtggcttc tcgaccctct
      301 acccggaact ctccaatctc gaggtactcg gcaccgcccg tcgcggctat ttcgtcgagg
      361 gactcggcgg cgcccagttc gccctttccg gcgcggtgga gcgcctgcgg gccctgccgg
      421 ccgaggagaa tggtcccgac accttcacca tccttgccgc taccgacccg gccagtccct
      481 ggggctcgac cctgccctgg cccaaactcg agtccggtcg caaggccgcc cgtgcggccg
      541 gcgcctacgt gctcgcccgc aacggccacc ccctgctcta cgtggaacgg ggcggcaaag
      601 gcctgcttcg actggatcct tccctggaag gggaggctct cgccgcggcc ctttcggccc
      661 tgaccgagga ggccaacgcc ggacggctcg gccagctcaa gatcgagcgc ttcgacggcg
      721 agccgattct cggctcgaca ttcgaacagc ttctcgtcgc ggccgggttc agccgccagc
      781 ctcgccgcct ggtcgccccc ggctgatggc cgagggaatt ttcggtgcct cggccccgat
      841 tgatcgggaa cgtcttgtct ttccgccccg atgcgggata atccgccggt gacttcggcc
      901 gaggagagca cagtcgggct gctgagaagc gtgccccttt tcgccgagct tgacccggac
      961 gagctcgagc agttttcccg tgtcgccatt ccccgctcct atccggcggg caccagggtc
     1021 ttccacgagg gcgaccattc ggacgcctgc tacatcgtca agtcgggaac cttccgggtg
     1081 acccgcgagc atcccgacgg gcgggctatc acccttgcca ccctggggga gggcgacatc
     1141 gtcggcgagc tggcgatgct tgacggcgag gtccgttccg cctcggtcga gaccctcggc
     1201 ggtgacggcg agctgctggc cctgccggcc aacgacgtcc gttccctgct tgagcggcac
     1261 ccggagatca cggtcaagtt cgtcgctgcc ctgacccggc gtctgaggtc cgccaacgag
     1321 cggatctccc gccagtcctt ccagaccgtg ccctcgcgcg tggccggcgt gctctcgcag
     1381 atggtcgcgg aagagggtga ggacgggcgg caggtgacga tccggatgaa ccaggccgac
     1441 ctggcccagc tggccggcac ctcccgggag agcgtcagcc gctttctcgc ggacctcgag
     1501 cgttccggtg tggtcaaggc gggaaggggc caggtgacca tcctggaacc cgcccggctc
     1561 ggcaactaca tattctaggt cggcgaaatg accggacttc tggctctcaa cttctattcg
     1621 cccctcttcg tggatcagct caggcgtggt cgcaagacgg cgacgatccg cctcggcaac
     1681 aagtcgggca agtaccagcg cagccagatc gtctgggtca ccgtcggctc ccgacactcc
     1741 cgtcgcgaga agatcttctc cgccgtcatt gacgaggtcg aggtcaaacg gctgtccgag
     1801 ctctcgcccc gcgacatcga gcgggacaat cccgagttcc gccgggttga cgagaccagg
     1861 aacttcctcc agaagatcta tgaccggccg atcgaggagg acgcgatcgt caccgtggtc
     1921 cacttctcgc agatcgtcga gccgggcctg accggttacg gcaccgtccc gtccccgaac
     1981 tagatgtccg ggtcggggga ctcggctgcc ttcgctttcg tctcgcgctg ggagctctcg
     2041 gcccctcgtc agccggtctg ggacgccctg gtcgacttca acgcctggcc ggtctggtgg
     2101 ccgggactgg aatcggtcat cgagacgatc cac
//
LOCUS       DAOTIP010000375         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61471_length_2132_cov_3.656716, whole genome shotgun sequence.
ACCESSION   DAOTIP010000375 DAOTIP010000000
VERSION     DAOTIP010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61471_length_2132_cov_3.656716"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5138_07505"
     CDS             <1..183
                     /locus_tag="P5138_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRV60462.1"
                     /translation="ADPDGKRSRLTIELHEAGPGSTEVTLTHDRLGEAPPGGHVGVNA
                     GWTQALDSLGRYFEND"
     gene            194..871
                     /locus_tag="P5138_07510"
     CDS             194..871
                     /locus_tag="P5138_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HRV60463.1"
                     /translation="MTLQPTVSADEWESARQKLLIREKEVTRAKDALAAERRRMPWLK
                     VEKDYEFEGPDGRVRLLDLFEGRRQLIIYRFFFDPEVTGHPDAGCPGCSFGADQVADL
                     RHLNARDTTFCHVSRGSQENIGRLKEKMGWQQIPWYTVIDDFDRDMGVGEWHGTNAFI
                     RDDDGTIYRTYFIDARGDEQMGSTWAHLDLTALGRQEEWEDSPEGYPQTPPYQWWRRH
                     DEYEPVS"
     gene            complement(982..1776)
                     /locus_tag="P5138_07515"
     CDS             complement(982..1776)
                     /locus_tag="P5138_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60464.1"
                     /translation="MSEFTPFHRGGSGEPLVLIHGFTGTWEMWDLVIAPLEEKYDVFI
                     PTLPMHAGGPEIEGEASVDLLIEGTETLLDEVGIETAHLVGNSLGGYAALRLAANGRA
                     RTVNALAPAGGWAEGDPVIDQTMDFFQEMIPLVAAAAEHVDDAVATPEGRAMLLQFLT
                     EHPEVVSPELSKKILLGGAGCTEALALAEMGRTTDWSFDYDAIDCPTRIVWGTEDKVL
                     GWPVAAARFQEGLSDADWIILEGIGHCPMLDVPEKTVELITEFARD"
     gene            1845..>2132
                     /locus_tag="P5138_07520"
     CDS             1845..>2132
                     /locus_tag="P5138_07520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRV60465.1"
                     /translation="MPAETTHRMRVRYNECDRVGIVFNSNYLVYTDVAATEFWREHLG
                     GYSEFNEIGLDMVLAEANIRFRQPLEFDDEFDIRVSVGGMTARSMTIEFEFT"
BASE COUNT          408 a          694 c          669 g          361 t
ORIGIN      
        1 gccgacccgg acggcaagcg ttcccgcctg accatcgaac ttcacgaagc ggggcccgga
       61 tcgaccgagg tcaccctgac ccacgaccgg ctcggcgaag caccacccgg cggccacgtc
      121 ggagtcaacg ccggctggac ccaggctctc gacagcctcg gccgctattt cgaaaacgat
      181 tgagaggaac gacatgactt tgcagccaac ggtttcggcc gatgaatggg agtcggcccg
      241 gcagaagttg ctgatcaggg aaaaggaagt gacccgggcg aaggacgcgc tggccgccga
      301 aaggcgccgc atgccctggc tgaaggtcga aaaggactat gagttcgagg ggccggatgg
      361 cagggtcagg cttcttgacc tcttcgaggg acgccggcaa ctgatcatct accggttctt
      421 cttcgacccc gaggtgaccg gccatcccga tgccggctgt cccggctgct ccttcggggc
      481 cgaccaggtc gccgacctcc ggcacctcaa cgcccgggac acgaccttct gccacgtctc
      541 ccggggctcg caggagaaca tcggccgcct gaaggagaag atgggctggc agcagatccc
      601 ctggtacacg gttatcgacg acttcgacag ggatatgggc gtcggcgagt ggcacggaac
      661 caacgccttc atacgcgacg acgatggcac gatctaccgc acttatttca tcgacgcccg
      721 cggcgatgaa caaatgggaa gcacctgggc tcacctcgac ctgaccgcac tcggccgcca
      781 ggaagagtgg gaggactcgc ccgaaggcta cccccagacc ccgccgtacc agtggtggcg
      841 tcgccacgac gagtacgagc cggtctcatg atcctcgccc acatcgccgg gatcccggtc
      901 gaggagatgc taatgctggc ccccgccttc gcgggggcgg ggatagccct tcgctcccgc
      961 ctgaggcggg agcgaaggta gtcagtcgcg tgcgaattcg gtgatcaact cgaccgtctt
     1021 ctcgggcacg tcgagcatcg ggcagtggcc gattccttcg aggatgatcc agtcggcgtc
     1081 cgacaggcct tcctggaagc gggcggcggc gacgggccag cccaggacct tgtcctcggt
     1141 accccagacg atccgggtcg ggcagtcgat cgcgtcgtag tcgaaactcc agtcagtggt
     1201 gcgacccatc tcggcaagcg ccagcgcttc ggtgcatccg gcaccgccca gcaggatctt
     1261 cttcgacagt tccggcgaaa cgacttcagg gtgttcggtc aggaactgaa gcagcatcgc
     1321 ccggccttcc ggggtggcaa cggcatcgtc gacgtgttcc gccgccgcgg caaccagtgg
     1381 gatcatctcc tggaagaagt ccatggtctg gtcgatcacg gggtcacctt cggcccagcc
     1441 gccggccggg gcgagcgcgt tgacggttct cgccctgccg ttcgccgcga gtcgaagtgc
     1501 ggcatagccg ccgagtgagt tgccgacaag gtgggcggtt tcgatcccca cctcgtcaag
     1561 caaggtttcg gtcccttcga tcagcaggtc gacactcgcc tcgccttcga tctcagggcc
     1621 gccggcgtgc atcggcaggg tcgggatgaa tacatcgtat ttctcctcca ggggagcaat
     1681 gaccaggtcc cacatctccc aggtcccggt gaacccgtga atcagaacca gcggttcgcc
     1741 gctgccacca cgatgaaaag gcgtaaattc gctcatgcct acattctcca ccctcggatg
     1801 gaattcgggg tcgttccaga aagcggatag gctcgcagcc gcaaatgcca gccgagacaa
     1861 cccacaggat gcgggtccgt tacaacgaat gtgaccgggt cgggatcgtc ttcaactcga
     1921 actacctcgt ctacacggat gtggccgcga ccgagttctg gcgcgaacac ctcggcggct
     1981 actccgagtt caacgagatc ggactggaca tggtcctggc cgaagcgaac atccgcttcc
     2041 gccagccgct cgagttcgat gacgagttcg acatccgggt gagcgtcggc ggtatgaccg
     2101 cccgctcgat gacaatcgag ttcgagttca cc
//
LOCUS       DAOTIP010000376         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_61611_length_2129_cov_2.916104, whole genome shotgun sequence.
ACCESSION   DAOTIP010000376 DAOTIP010000000
VERSION     DAOTIP010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61611_length_2129_cov_2.916104"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5138_07525"
     CDS             <1..220
                     /locus_tag="P5138_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRV60466.1"
                     /translation="PEDEDYETVAGLIGFELGRVPGRGDSVRVEATNEDREPLLVDLY
                     VLRMDGLRVDRVRLVVSRPEPEEGDEQK"
     gene            217..1278
                     /locus_tag="P5138_07530"
     CDS             217..1278
                     /locus_tag="P5138_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRV60467.1"
                     /translation="MSTPLALTISLFLLAGNAFFVGAEFALISARRSVIEPRADEGGW
                     SARIAIRAMERVSLMMAGAQLGITLCTLGLGALAEPAIAHGLEGVFADIGIPHSLVHP
                     VALIFALFLVGGLHVVLGEMVPKNIALAGPDRSVLMLAPPMAAIVKILHPVIWVLNLT
                     ANGVLRLIGVTPSDEVTSAFTRDEVAGLVEESRREGMLDADETRLLTGALTFEERDAS
                     VVLLPIDTIVTLPITVTPAEVEEAAGRTGFSRFPVTRDGEMIGYLHLKDALEVKEKHR
                     NRPIAESWIRPLPTVRLTDQLREVLEAMQSSGAHLARVVVRGSRGRGKTVGVVALEDV
                     LEELIGEVRDAAQQIESSK"
     gene            1288..1746
                     /locus_tag="P5138_07535"
     CDS             1288..1746
                     /locus_tag="P5138_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRV60468.1"
                     /translation="MSANDEVIARFYGAFAAGDADTMAGCYHERIHFHDPVFQDLDGP
                     EVMKMWRLLLGRSDDLEITLGEHQASGDSGSAHWSAVYTFSQTGRVVHNEIDAAFRFE
                     DGLIIDHRDSFDFWKWTRMALGVPGVLLGWSPIVQGKVRKQSAELLAGAG"
     gene            1755..>2129
                     /locus_tag="P5138_07540"
     CDS             1755..>2129
                     /locus_tag="P5138_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV60469.1"
                     /translation="MVGTGEAVTENLLCIRPGASTATDYEVLFADPEVERWLRPDPMP
                     RFIRADFDRMARRDRAHWDRYGYGPWAVREREGGAFVGRGGLAWATVAGEREVELPWA
                     VRPALQNHGYATEMALAAIEAAR"
BASE COUNT          339 a          689 c          738 g          363 t
ORIGIN      
        1 gcccgaggac gaggactacg aaacggtggc tggccttatc ggtttcgaac tcgggcgggt
       61 tccgggccgc ggcgactcgg tccgtgtcga agcgacgaac gaggacaggg aaccgctgct
      121 tgtggacctc tacgtgctgc ggatggacgg gctacgggtt gaccgggtgc ggctggtcgt
      181 ctcccggccc gagccggagg aaggggacga gcagaaatga gcactccgct cgccctgacc
      241 atctccctct tccttctggc cggcaacgcc ttcttcgtcg gggccgagtt cgcgctgatc
      301 tcggcccgtc gttcggtgat cgaaccacgg gccgacgaag gcggctggtc ggcccggatc
      361 gcgattcgcg cgatggaacg ggtatcgctg atgatggccg gggcgcaact cggaatcacc
      421 ctctgcacac tcggattggg tgccctggcc gaaccggcga tagcccacgg ccttgagggg
      481 gttttcgcgg acatcggcat cccgcattcg ctggttcatc cggtcgccct gatcttcgcg
      541 ctcttcctgg tcggcggtct ccacgtggtg ctcggcgaga tggtgccgaa gaacatcgcc
      601 ctggccggac ctgaccgctc ggtcctgatg ctcgccccgc cgatggcggc gatcgtgaag
      661 atcctgcatc cggtcatctg ggtgctgaac ctcaccgcaa acggggtgct gcgactgatc
      721 ggagtcaccc cttcggacga ggtcaccagt gccttcaccc gcgacgaggt ggccggcctg
      781 gtcgaagagt cgcgtcgtga agggatgctc gacgccgatg aaacccgcct cctgaccggc
      841 gccctcacct tcgaagagcg ggacgcgagc gtcgtgctgc tgccgatcga cacgatcgtg
      901 accctcccga tcaccgtcac cccggccgag gtcgaggagg cggctggccg gaccggcttc
      961 tcccgcttcc cggtgaccag ggacggtgag atgatcggtt acctccacct caaggatgcc
     1021 ctggaggtga aggagaagca ccgcaaccgc ccgatcgcgg aatcctggat aaggcccctg
     1081 ccaacggtcc gtctcaccga ccagttgcgc gaagtcctgg aagccatgca gagttccggg
     1141 gcccatctcg cccgggtcgt cgtccgggga tcccggggga ggggcaagac ggtcggggtc
     1201 gtcgccctcg aggacgtgct ggaagagctg atcggcgagg tgagggatgc cgcccagcag
     1261 atcgagtcgt cgaagtagga tcgccccatg agcgctaacg acgaggtgat cgcacgtttc
     1321 tacggtgcat tcgcagcagg tgacgccgac acgatggcgg ggtgctacca cgagcggatc
     1381 cactttcacg acccggtctt tcaggatctg gacggtcccg aggtgatgaa gatgtggcgc
     1441 ctgctcctgg gccgctcgga tgacctggaa atcaccctcg gcgagcatca ggcgagcggc
     1501 gattccggct ccgctcactg gtcggccgtc tacaccttca gccagaccgg ccgggtggtc
     1561 cacaacgaga tcgacgccgc cttccgtttc gaggacggcc tgatcatcga ccaccgcgac
     1621 agcttcgact tctggaagtg gacccggatg gcgctcgggg tgcccggggt tctgctcggc
     1681 tggagcccga tcgtccaggg caaggtccgg aagcagtccg cggaactgct ggccggggcc
     1741 ggctgaggct cctcttggtc ggcacgggcg aagcggtcac cgaaaacctg ctctgcatcc
     1801 ggccgggggc ttcgacggcc accgactatg aagtcctgtt cgccgacccc gaggttgagc
     1861 gatggctgcg gccagatccg atgccgcgct tcatcagggc cgatttcgat cgcatggccc
     1921 gccgcgatcg cgcccactgg gaccggtacg gctacggccc gtgggctgtc cgggagaggg
     1981 aaggcggtgc cttcgtcggc cggggcgggc tggcctgggc cactgtcgcg ggtgagcggg
     2041 aagttgagct tccctgggcc gtgagaccgg ctctccagaa ccacggctac gcgaccgaga
     2101 tggcgctcgc ggctatcgaa gccgcccgg
//
LOCUS       DAOTIP010000377         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_62292_length_2112_cov_3.166748, whole genome shotgun sequence.
ACCESSION   DAOTIP010000377 DAOTIP010000000
VERSION     DAOTIP010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62292_length_2112_cov_3.166748"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="P5138_07545"
     CDS             <1..1057
                     /locus_tag="P5138_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRV60470.1"
                     /translation="GRVPNANVSIEKLAGEFRAHTEIPIPVRFNPKFLNTLGIEDGET
                     MQGRLGTAHPHFDPETRERFSYEVELVPPSGLRIVSEKGKSRRELAFIPQSNPGYLHS
                     FGLTRRFVVIVSQPFNFNLAKFLSPDRGPIITNYEWKGEEPTRIILVDRKRGGVARTV
                     ETDSFFAFHHINAYEHDGKVVIDVCAHKDAGIIDALYLKKIRAGNKVPQARYRRFEVR
                     LSNGRLTQRDLIDAYIELPQKNYSRVNGKRYRYAYGVGTRKGSSGFIDQLVKADVKKG
                     ESKLWREWGVYPGEPVFVPRPGAKAEDDGVLLSVVLDGRRRKSGLLVLDARNMEEIAR
                     ASVPHHIPFGFHGLYAR"
     gene            1118..>2112
                     /locus_tag="P5138_07550"
     CDS             1118..>2112
                     /locus_tag="P5138_07550"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRV60471.1"
                     /translation="MERVPPTKNAGSFVLVTSLTALVLVVPCLAAIGLRASGLVTSTA
                     LLILIPVVVSVLFSHSIAEFWKRRRTGSPLLFEDLMLWGWLRRRRFEKLLARSEDFVG
                     PEAGRGLSPERRARELERLAGALEARDPRTHGHSRRVARHATTIARSLKLETAEVARV
                     RTAALLHDVGKIEVPWEILEKPGALTDEEFAEIRKHPVAGASLVEGMDDPELTAIVRH
                     HHERIDGGGYPDGIVADEIPIGARIIAVADTFDALTSARSYRQPRSHEEALEVLREEA
                     GTQLDSNAVEAFDGRYSSRRPVALVAAALGLGRLAGQSLVSFGTGASQVVAVGAAA"
BASE COUNT          360 a          700 c          693 g          359 t
ORIGIN      
        1 cgggcgggtc ccgaacgcca acgtctcgat cgagaaactg gccggcgagt tccgggccca
       61 tacggagatt ccgatcccgg tcaggttcaa tccgaagttc ctgaacaccc tgggaatcga
      121 agacggcgaa acgatgcagg gccggctcgg aaccgcccat ccgcatttcg atcccgaaac
      181 ccgggagcgc ttctcctacg aggtggaact ggtgccgccg agcggtctga ggatcgtctc
      241 ggagaagggc aagagccggc gggagctcgc cttcatcccg cagtccaacc ccggctacct
      301 gcattccttc ggactcaccc ggcgcttcgt ggtcatcgtt tcgcagccct tcaacttcaa
      361 tctcgcgaag ttcctcagcc cggaccgggg tccgatcatc accaactacg aatggaaggg
      421 cgaggaaccg acccggatca tcctggtcga ccgcaagcgc ggcggtgtcg cccgcaccgt
      481 cgagaccgac tcgttcttcg ccttccacca catcaacgcc tacgagcacg atggcaaggt
      541 ggtcatcgac gtctgcgccc acaaggacgc cgggatcatc gacgccctct acctgaagaa
      601 gatccgggcc ggcaacaagg tgccccaggc ccggtatcgc cgcttcgagg ttcgtctctc
      661 caacggccgc ctcacccagc gcgacctgat cgacgcctac atcgaactcc cgcagaagaa
      721 ctattcacgg gtgaacggaa agcgctaccg ctatgcgtac ggggttggta cgcgcaaggg
      781 ctcgagcggc ttcatcgacc agcttgtcaa ggcggatgtg aagaagggcg agtcgaagct
      841 ctggcgggag tggggcgtct acccgggcga gccggtcttc gtgccgcggc cgggggcaaa
      901 ggcagaagat gacggcgtcc tgctctcggt cgtgctcgac gggcgccgga gaaagtcggg
      961 gctgctggtt ctcgacgccc gcaacatgga ggagattgcc cgggcttcgg ttccccacca
     1021 catacccttc gggtttcacg ggctgtatgc tcggtgaaca ggggtgtttc tttttctgac
     1081 tgacgggtag aaaccgacct caggctccgg agggctgatg gaacgcgtcc cgccaacgaa
     1141 gaacgcaggc agttttgtgc tggtgaccag ccttacggcg ctggtcctgg tcgttccctg
     1201 cctcgccgcg atcggtctcc gcgcaagcgg actcgtcacc tcaacggcgt tgctgatcct
     1261 gattccggtc gtggtgtcgg tcctcttttc ccattcgata gccgagttct ggaagcggcg
     1321 ccgcacggga agccccctcc tcttcgagga cctgatgctc tggggctggc tgcgccggcg
     1381 aaggttcgag aagctgctcg ccaggtccga ggacttcgtc ggccctgagg ccggccgcgg
     1441 cctcagtccc gagcggcgcg cccgggaact ggagcgactc gccggtgcct tggaagcccg
     1501 ggatccgcgc acccatggac actcccggcg ggtcgcgcgt catgccacca cgatcgcccg
     1561 ctcgctgaag ctggagaccg cggaggtcgc ccgggtccgc accgctgccc tgcttcatga
     1621 cgtcggcaag atcgaagtgc cctgggagat cctcgagaag cccggcgccc tgaccgacga
     1681 ggagttcgcg gagatcagga agcacccggt tgccggcgca agtctggtcg agggcatgga
     1741 cgatccggaa ctgaccgcga tcgtccggca ccaccacgaa cgaatcgatg gcggcggcta
     1801 cccggacggc atcgtggccg acgagatccc gatcggtgcc cggatcattg ccgtggccga
     1861 caccttcgac gcgttgacct cggcccgctc ctaccggcag ccgcgctcgc atgaggaggc
     1921 gctcgaggtg cttcgcgagg aagccgggac acagcttgac tccaacgcgg ttgaggcctt
     1981 tgacggccgc tattccagcc gccgtccggt ggcacttgtc gcggcggctc tcgggctcgg
     2041 gcgtctggcc ggccagtccc tggtcagctt cggcacggga gcgagccagg tggtcgcggt
     2101 gggggcggcg gc
//
LOCUS       DAOTIP010000378         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_62327_length_2111_cov_3.890564, whole genome shotgun sequence.
ACCESSION   DAOTIP010000378 DAOTIP010000000
VERSION     DAOTIP010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62327_length_2111_cov_3.890564"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2111)
                     /locus_tag="P5138_07555"
     CDS             complement(<1..>2111)
                     /locus_tag="P5138_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV60472.1"
                     /translation="EPAPSPRRPARETPAPTPRGIGLEIDPRIEGGYLVGRKRILEQL
                     GSELDLAAAGELRISLLAGEGGVGKTRLAAELAASRDDITVLYGRSEPDEVRPFRIWS
                     GLLQSAVRQLGDVDPADIVGGDGPTLSRLLPELARRMDLPDPGPTSDLESERQALFGA
                     VMRMIGRFSARRPMLIILDDLHWADRSTLRLLASMAGDNPLRSILALGIYRDTELPAS
                     SHLPDTLSQLQRRLPTTRIQVEALDEEEVKSLISGRLDESLAAVVRDQTGGNPFFIEQ
                     LVRNLEESGEDHPGAVPPEIREIITQRVSRLPEGGPELLGRAALIGRDFDLEILRRTT
                     TDDDDRIIELLDASVGAGLLDESGSVPGRYSFVHALVRSSLEDSFGLTRRSAVHRRIG
                     EAIESRNQNRFGDQRDEDLPTLAWHFTHAGPAEADRAIHYATLSARQAEERLAYDEAV
                     DFLGGAIAACRADEPVDQGLLAELLLSRAEAEWRMALLQRSGRTFGEAAEAARESGIP
                     ELIARAAMGTTWGSWDSFESDRDVQGRLLREALEAIGPGDSPFRAQLLASLAQILYFS
                     GGLSADARAMAIEATEMAERVNDPYTFFVSNIAASFLRWEPEGRASRLPMADRLVEVA
                     ESSEDPEDLAEALSWRAVVQLNLGHRAEADADIARFDVLARTLPQIRVNQAGLHSSQM
                     VLEGRWDEAAQLITETIQQDIP"
BASE COUNT          324 a          710 c          732 g          345 t
ORIGIN      
        1 gggatgtcct gctgaatggt ctcggtgatc agctgcgcag cttcatccca ccttccttcg
       61 aggaccatct ggctcgaatg aaggccggcc tggttgaccc ggatctgcgg cagggttctg
      121 gccaggacat cgaaccgggc gatgtccgcg tcggcttccg cccggtgacc caggttcaac
      181 tgcaccaccg cacgccaact cagggcctcg gccaggtcct cgggatcctc cgacgattca
      241 gctacctcga ccagccggtc ggccatcggc agacggctgg cgcggccctc cggctcccac
      301 ctcaggaacg atgcggcgat gttcgagacg aagaaggtat aggggtcgtt cacgcgttcc
      361 gccatctcgg tcgcttcgat cgccatcgcc cgggcatcgg ccgacaatcc cccgctgaaa
      421 tagaggatct gggccaggct ggcaagtagc tgtgcccgga aaggactgtc acctggcccg
      481 atcgcttcga gggcctcgcg tagcagcctg ccctggacgt cgcggtcgct ttcgaaggag
      541 tcccagctgc cccaggtggt gcccatcgcc gcccgggcga tcagctcggg aatcccggat
      601 tccctggccg cttccgccgc ctcaccgaag gtccggccgg accgctggag gagcgccatc
      661 cgccactctg cctccgctcg gctgagcaac aactcggcca gaagaccctg gtcgacgggt
      721 tcgtcggccc ggcaggcggc tatggcgcca cccaggaaat cgaccgcctc gtcgtaggcc
      781 aggcgttcct cggcctggcg ggccgaaagg gtggcgtagt gaatggcccg gtcagcctcg
      841 gccggcccgg cgtgggtgaa atgccaggcc agggtcggaa gatcctcgtc tcgctggtct
      901 ccgaaccggt tctggttgcg ggactcgatc gcctcaccga tccgacggtg aactgcggat
      961 cttcgcgtga gcccgaagct gtcctcgagt gaactcctga ccagggcgtg aacgaacgag
     1021 taacggccgg gaacggaacc ggattcatcg agcaggccgg ccccgaccga cgcgtcgagc
     1081 aattcgatga tccggtcgtc atcgtcggtt gtggtccggc gcaggatctc gaggtcgaaa
     1141 tcgcgaccaa tgagggcggc ccggccgagc agttcgggtc cgccctcggg caggcgggat
     1201 acccgctggg tgatgatctc ccggatctcc ggcggcaccg ccccgggatg gtcctccccg
     1261 gattcctcga ggttgcgcac gagctgttcg atgaagaagg ggttgccgcc ggtctggtcg
     1321 cggacgaccg cggcgaggct ctcgtcgagc cggcccgaga tcaggctctt gacctcttcc
     1381 tcgtcgaggg cttcgacctg gatgcgggtg gtcggcagtc gccgttgaag ctggctgagg
     1441 gtgtcgggca ggtggctgct ggcgggcagc tcggtgtcgc ggtagatgcc gagcgcgagg
     1501 atgctccgca gggggttgtc cccggccatg ctggccagca gccgaagggt cgagcggtcg
     1561 gcccaatgca ggtcgtcgag gatgatcagc atcggccgcc tcgccgagaa gcggccgatc
     1621 atccgcatca cggcaccgaa cagcgcctgg cgctccgact cgaggtccga ggtcggcccc
     1681 ggatcgggca ggtccatcct gcgggccagc tccggcagga ggcgcgaaag ggtcgggccg
     1741 tcaccaccga cgatgtcggc cgggtctacg tcaccgagct gccggaccgc ggactggagc
     1801 aggcccgacc agatcctgaa tggccggacc tcatccggct ccgagcggcc gtagaggacg
     1861 gtgatgtcat cccggctggc ggcgagttcg gccgcgaggc gggtcttgcc gaccccgcct
     1921 tctcccgcga gcagggaaat cctgagttcg ccggccgcgg caaggtccag ttcgctcccc
     1981 agctgctcga gaatccgctt gcgcccgacc agatagccac cctcgattct cgggtcgatc
     2041 tccagaccga tgccccgggg ggtgggtgca ggcgtctccc gggccggccg ccgcggggag
     2101 ggagccggtt c
//
LOCUS       DAOTIP010000379         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_62412_length_2109_cov_3.024830, whole genome shotgun sequence.
ACCESSION   DAOTIP010000379 DAOTIP010000000
VERSION     DAOTIP010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62412_length_2109_cov_3.024830"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="P5138_07560"
     CDS             <1..734
                     /locus_tag="P5138_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRV60473.1"
                     /translation="FALETDVTRLVDAGLVSKDWQNNKYKGIVTNSVVSIVTRPGNPE
                     GIESFQDIIDKDVSVVTPNPFTSGGARWNIMAIYGAALHEGKSEQEALDTVKQVLSQA
                     EVQPASARDALQAFVGGAGDVLISYENEAIAAQNAGEDVDYVIPDSTILIEQPAAVTV
                     DAPKSATDFLNYLWSDAGQKIWAEQGYRPVNKSLVDESVYPTPKDEFTIAEFGGWDKV
                     ADEFFDDKTGSIAEIEKELGVSTEG"
     gene            799..1563
                     /gene="cysT"
                     /locus_tag="P5138_07565"
     CDS             799..1563
                     /gene="cysT"
                     /locus_tag="P5138_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRV60474.1"
                     /translation="MTKGLVVTYLSLIVLIPLAAIAAKAFSAGPAEFWDAIRQPQAIA
                     ALKLTAICSLIVVAINAVMGTLIAWVLVRDEFPGKSIVNSMIDLPFALPTIVASLTLL
                     TLYGNDSFLGIHAAYTKFAVIIALLFVTLPFVVRAVQPLLLSLDRDMESAAESLGAGK
                     VRIFTQIIFPNLLPGIAAGVALAFARALGEFGALVLISGNVPYKTEVASVFIRGQIES
                     DQVVGAAAVSVVLLVFSMCMLAFVNLIQKWGLRHDR"
     gene            1553..>2109
                     /locus_tag="P5138_07570"
     CDS             1553..>2109
                     /locus_tag="P5138_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60475.1"
                     /translation="MTASGRLSKYGLRFVALFYLTALLLLPIGVALVKTFEDGIGEVI
                     KQITEPDAIHALKMSLLTVIIAVPLNTVFGIASGAFAKSSALTSESFNMPRKMHSTPG
                     CPTWAAFASAEARIMTNCIVIDVSIHASICFLPTAVSSVRSGSVPLTLRGRPPASASH
                     SPCFEDRSSARRTLLPCSLGEAIER"
BASE COUNT          364 a          715 c          614 g          416 t
ORIGIN      
        1 acttcgcgct cgagaccgac gtgacccgtc tggttgatgc cggcctcgtc tcgaaggact
       61 ggcagaacaa caagtacaag ggcatcgtga ccaactcggt cgtctcgatc gtgacccgtc
      121 ccggcaaccc ggaaggcatc gagtccttcc aggacatcat cgacaaggac gtaagtgtcg
      181 tgaccccgaa cccgttcacc tccggtggcg cccgctggaa catcatggcc atctacggtg
      241 ccgccctgca tgagggcaag agcgagcagg aagcccttga caccgtcaag caggtcctct
      301 cgcaggccga agtccagccg gccagcgccc gtgacgccct ccaggccttc gtcggcggcg
      361 ccggtgacgt cctgatctcc tacgagaacg aggcgatcgc cgcgcagaac gccggcgagg
      421 acgtggacta cgtgattccg gattcgacga tcctgatcga gcagccggcc gcagtcacgg
      481 tcgatgcccc gaagtccgcg accgacttcc tcaactatct ctggagcgat gccggccaga
      541 agatctgggc cgagcagggt taccgcccgg tcaacaagag cctggttgac gagtccgtct
      601 acccgacccc gaaggacgag ttcacgatcg ccgagttcgg tggctgggac aaggtcgccg
      661 acgagttctt cgacgacaag accggctcga tcgccgagat cgagaaggaa ctgggagttt
      721 ccactgaagg ctgaatccac agccgtagcg gcacctcgac cccgggcggg cctgcgcctg
      781 cccggggttg aaaccgcctt gaccaagggc ctggtcgtca cctacctgag cctgatcgtg
      841 ctgatcccgc tggcggcgat tgcggccaag gctttctcgg cgggtccggc cgagttctgg
      901 gacgcgatca ggcagccgca ggcgatcgcc gccctgaagc tgaccgcgat ctgctccctg
      961 atcgtcgtgg cgatcaacgc ggtgatgggc accctgatcg cctgggtcct ggtccgggac
     1021 gagttccccg gcaagagcat cgtcaactcg atgatcgacc tgcccttcgc cctgccgacc
     1081 atcgtcgcca gcctcaccct gctcacgctc tacggcaatg acagcttcct cgggattcac
     1141 gccgcctaca caaagttcgc ggtgatcatc gccctgctct tcgtgaccct tcccttcgtg
     1201 gtgcgggccg tccagccgct gctgctgtcc cttgaccgtg acatggagtc cgccgccgaa
     1261 tcgcttggcg ccggcaaggt ccgcatcttc acgcagatca tcttccccaa cctgttgccc
     1321 ggcatcgccg ccggcgtcgc actcgccttt gcccgcgcgc tcggtgaatt cggcgccctc
     1381 gtgctgatct cgggcaatgt gccctacaag accgaggtcg cctcggtctt catccgtggc
     1441 cagatcgaat ccgaccaggt ggtcggtgcg gccgccgtct cggtcgtgct cctggtcttc
     1501 tcgatgtgca tgctggcctt cgtcaacctg atccagaaat ggggtctgcg tcatgaccgc
     1561 tagtggccga ctctccaaat acgggctccg gttcgtcgcg ctcttctacc tgaccgctct
     1621 gctgctgctg ccgatcggcg tggccctggt caagaccttc gaggacggga tcggtgaagt
     1681 gatcaaacag atcacggagc cggacgcgat ccacgccctg aagatgtcgc tgctcacggt
     1741 gatcatcgcg gtgccgctca acacggtctt cggcatcgcc tccggcgcct tcgcaaagtc
     1801 ttcggccttg acctcggaga gtttcaatat gccgaggaaa atgcactcca cgcccggttg
     1861 cccgacctgg gccgccttcg cttcggcgga agcgcgcatc atgacgaatt gcatagtaat
     1921 cgacgtttcc attcatgcct ccatttgttt tcttccgacc gccgttagtt ccgtccggtc
     1981 cgggtcagtt cctttaactt tgcgcggacg ccctccggcc tcagccagcc atagcccctg
     2041 ttttgaagat cgatcatcag cgcgccgaac tcttcttccg tgcagcttgg gtgaagcaat
     2101 cgaaagatt
//
LOCUS       DAOTIP010000380         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_62527_length_2106_cov_3.485617, whole genome shotgun sequence.
ACCESSION   DAOTIP010000380 DAOTIP010000000
VERSION     DAOTIP010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62527_length_2106_cov_3.485617"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="P5138_07575"
     CDS             complement(<1..1380)
                     /locus_tag="P5138_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRV60476.1"
                     /translation="MEASGAGVSESAATNGTGPGGGGEPRVCVIGAGSSGITAMRSLK
                     EKGIDHVCFEIGSDVGGLWRYENDNGRSAAYKSLHINTSRQMMAYDAFPMPEDYPDYP
                     DHAQITSYFEQFTDHFGLRDAIRFQTEVESVVPSEGGGWDVSWKNREGETGTEHFTDV
                     MVCSGHHWDKRWPEPAFPGSDSFAGLQMHAHDYRTPEIFEGKRVLVLGIGNSATDIAV
                     ESSRHAEATFLAMRRGAWVVPKYIGSKPTDHHTTGFMSRMPIAVQSLGMSLMVRMSVG
                     RPTDYGLPKPDHKLLHAHPTVSQDLYARLGHGDIKVKPNIERLDGDSVVFEDGSRERI
                     DVIVYCTGYRITFPYLAPEVFEARDNKVDLFMRVASPDQKGLYFIGLIQPLGAIMPLA
                     EVQCDWVADLVSGEGKLPDRLEMYREIADDQARMGRRFVKSKRHTVEVDFEPYMRAIA
                     KERSRSRDRS"
     gene            complement(1522..>2106)
                     /locus_tag="P5138_07580"
     CDS             complement(1522..>2106)
                     /locus_tag="P5138_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRV60477.1"
                     /translation="YLVAEVQRVYKAQGVEINDKHIEVIVRQMLKKVEVETKGSTELL
                     PGQLEDRQILKQINKQVKADGGTAAKAQEKILGITKASLATKSFLSAASFQETTKVLT
                     DAALEGKRDRLVGLKENVIIGKLIPAATGLKHYRSMTIEPTEPFAPAEEAVLLEEDLV
                     VENGDGANGADGFGESFADELDEISQEIDAGAEK"
BASE COUNT          329 a          668 c          662 g          447 t
ORIGIN      
        1 ggaccggtcg cgggagcgcg acctttcctt tgcgatggcc ctcatgtagg gctcgaagtc
       61 gacttccacc gtgtgacgtt tcgacttgac gaagcgccgg cccatcctgg cctggtcatc
      121 ggctatctcc ctgtacatct cgagtctgtc cggcagcttc ccctcgccac tgaccagatc
      181 ggcgacccag tcgcactgga cctcggccag gggcatgatc gccccgagcg gctggatcag
      241 cccgatgaag tagaggccct tctggtcggg ggatgcgacg cgcatgaaga ggtcgacctt
      301 gttgtccctg gcttcgaaga cctccggggc caggtacggg aaggtgatgc ggtagccggt
      361 gcagtagacg atcacgtcga tccgttcgcg gctgccgtct tcgaagacga ccgagtcacc
      421 gtcgagccgc tcgatgttcg gcttcacctt gatgtctccg tggccgagcc gggcgtagag
      481 atcctgcgag acggtgggat gcgcgtggag cagcttgtgg tccggcttgg gaagcccgta
      541 gtcggttggc ctgccgaccg acatgcggac catcaggctc atgccgagac tctggaccgc
      601 gatcggcatg cgggacatga atccggtcgt gtggtggtcg gtcggcttac tgccgatgta
      661 cttgggcacc acccaggctc cgcgccgcat ggccaggaag gtcgcttcgg cgtggcgtga
      721 ggattcgacc gcgatgtcgg ttgccgaatt gccgatgccg agcaccagca ccctcttgcc
      781 ctcgaagatt tccggtgtgc ggtagtcatg ggcatgcatc tgcagccccg cgaatgaatc
      841 cgagccgggg aaggcgggct cgggccagcg cttgtcccag tggtgcccgc tgcagaccat
      901 cacgtcggtg aagtgctcgg ttcctgtttc gccttcgcgg ttcttccagc tcacgtccca
      961 gcccccgcct tcggacggga ccaccgattc gacctccgtc tggaagcgaa tcgcgtcccg
     1021 cagtccgaag tgatcggtga actgctcgaa gtagctcgtg atctgggcgt gatccggata
     1081 gtccggatag tcctccggca tcgggaaagc gtcgtaggcc atcatctggc gggaggtgtt
     1141 gatgtggagc gacttgtagg cggccgagcg gccgttgtcg ttctcatacc gccagagtcc
     1201 gccgacgtcc gatccgattt cgaagcagac gtggtcgatt cccttttcct tgaggctgcg
     1261 catcgcggtg atccccgagg atccggctcc gatcacgcag actcgcggct cccctccgcc
     1321 accgggaccc gtcccgttgg tcgccgcact ttcagacacc cctgctcctg aagcttccat
     1381 ggtgagagtg tagcccaata ctgacaagga ttcatgtcaa ttttggtgga atcccggtct
     1441 gccggtaacc ccccagcacg actgatcctg caaagaaaag gggcgccctc aggcgcccct
     1501 tttcacgctt ctgtgccggt cctacttctc ggcgccggcg tctatctcct gtgagatctc
     1561 atcgagctca tcggcgaaag attcaccgaa gccgtcggct ccgttggctc cgtcgccgtt
     1621 ctcgactacg agatcctcct cgagcagaac cgcttcctcc gccggggcga agggctcggt
     1681 cggctcgatg gtcatcgagc ggtagtgctt gagaccggtc gcggccggga tcagcttgcc
     1741 gatgatgacg ttctccttga ggccgaccag acggtcgcgc ttgccttcaa gcgcggcgtc
     1801 ggtcaggacc ttggtggtct cctggaagga ggccgccgag aggaacgact tggtcgccag
     1861 cgaggccttg gtgatgccga ggatcttctc ctgggccttc gccgcagtgc cgccgtcggc
     1921 cttgacctgc ttgttgatct gcttgaggat ctgacggtcc tcgagctggc cgggcagcag
     1981 ttcggtcgat cccttggtct cgacctcgac cttcttcagc atctggcgaa cgatcacctc
     2041 tatatgcttg tcgttgatct ccacgccctg ggccttgtag acccgctgga cttccgcgac
     2101 caggta
//
LOCUS       DAOTIP010000381         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_62765_length_2100_cov_2.412225, whole genome shotgun sequence.
ACCESSION   DAOTIP010000381 DAOTIP010000000
VERSION     DAOTIP010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62765_length_2100_cov_2.412225"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1069)
                     /locus_tag="P5138_07585"
     CDS             complement(152..1069)
                     /locus_tag="P5138_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV60478.1"
                     /translation="MTVRQTIEIHGQPVTFHMMGEGPPVLLVHGITSSSRTWKAVMPR
                     LAEKYTVIAPDLLGHGRSSKPHGDYSLGAYASGMRDLLVALDVPKATVVGHSLGGGIA
                     MQFGYQFPDRVARLVLVDTGGIGPEVNPALRAAALPGAEFVLPLLFSPTLHDAGLKVR
                     NFFAGIGLHGNADVEGVAEGFASLTEADARRAFINTVRSVIDPSGQRVSAADRLYLTR
                     DIPSLIIWGDRDRIIPVAHADLAHELMPGSRLEIFPGAGHFPFNDDPDRFIRTLEEFI
                     ATTEEANLDQDHIRKMLLRKQAEDRAAAA"
     gene            complement(1098..>2100)
                     /locus_tag="P5138_07590"
     CDS             complement(1098..>2100)
                     /locus_tag="P5138_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRV60479.1"
                     /translation="NRSAAFWSSVATRFRDYPSVIFDLFNEPHSRWNADDTKVFTLGW
                     DCWANGGCYAPVEPDTAATSGHKWYRTTGLATLTEVVRNAGATQPIILSGIDYANDLR
                     GWLANAPDDDQLIAGFHNYPEQRCRTTACWNKEIAPLNEKVPVLAAEFGQNGCDRNGH
                     VNRFMDWADDHVIGYLAWAWWSLPDLGCHNFALVSDLDGTPLGAVGNALHDHLATLPA
                     VLPEPPVRVSPGLTIKKAKWKRPNLRLRIGISRKASKKAQVRVRLARDRKSSAGPRTT
                     RRLLRRTIVVKSGAGSLNLRIPSGLKPVRVVVYYPGDDLLLPKTVSRKPASVSKITR"
BASE COUNT          346 a          663 c          714 g          377 t
ORIGIN      
        1 ccttgacaca tgcttcggga tcaccctcgt tctcccggta gaggtcgatc gtcctctgct
       61 ggtactcctc cagcgcggtg agtacctcct cgaccggcag ctcttcgctc atgccccgag
      121 tatgccgcaa ccgctcgggt cggtcatctg gtcaggccgc cgcggcccgg tcttctgcct
      181 gcttgcgcag gagcatcttc cggatgtggt cctggtcgag gttcgcctcc tcggtggtgg
      241 cgatgaattc ctcgagcgtc cggatgaagc gatcgggatc gtcgttgaag ggaaaatgcc
      301 ccgcgcccgg gaagatttcg agccggctgc cgggcatcag ttcatgggcg aggtcggcat
      361 gcgcaaccgg gatgatccgg tcgcggtcgc cccagatgat cagcgaaggg atgtcccggg
      421 tcagatagag gcggtcagcg gcggagaccc gctggccgga aggatcgatc accgagcgca
      481 ctgtgttgat gaaggcccgg cgggcgtcgg cttcggtaag cgaggcgaac ccctcggcga
      541 cgccttcgac gtcggcattt ccgtgcaggc cgattccggc gaagaagttg cgcaccttga
      601 ggccggcgtc atgcagggtc ggcgagaaga ggagggggag cacgaattcg gcgccgggca
      661 gggcggcggc ccgcagggcc gggttcacct ccggtccgat ccctccggtg tcgacaagga
      721 cgagccgtgc cacccggtcc gggaactgat acccgaactg cattgcaatt cctcccccga
      781 gggagtggcc gacaaccgtg gctttcggca cgtcgagggc gaccagcagg tctcgcatgc
      841 cgctcgcgta ggcgcccagg gaatagtcgc catgcggctt cgaggaacgg ccgtggccga
      901 gcaggtccgg ggcgataacc gtgtacttct cggccagcct gggcatgacc gccttccagg
      961 tgcgggaact ggaggtgatc ccgtgaacca gcagcacggg cggcccctcg cccatcatgt
     1021 ggaaagtgac tggctggccg tggatctcga tcgtctggcg taccgtcatg tacccagtat
     1081 ccccgaatca gggggttcta acgggtgatt ttcgacacag aagccggttt ccggcttacc
     1141 gtcttcggca ggagcagatc gtcccccggg taatagacga cgacccggac cggcttcagc
     1201 ccggaaggaa tccggaggtt caggctcccg gcaccggatt tcaccacgat cgtcctgcga
     1261 agcagccgtc gggtcgtcct gggcccggcg gaagatttcc tgtcccgcgc cagccggacc
     1321 cgcacctgcg cctttttcga ggccttgcgg ctgatgccga tccgcaggcg aaggttcggg
     1381 cgcttccatt tcgccttctt gatcgtaagg ccgggggaga cccgcaccgg cggctcgggc
     1441 agaacagccg gcagcgtggc caggtgatca tgaagcgcgt ttccgaccgc ccccagaggg
     1501 gttccgtcga ggtcggagac cagggcgaag ttgtgacagc cgaggtccgg cagcgaccac
     1561 caggcccagg ccagataacc gatcacatgg tcgtcggccc agtccatgaa gcggttcacg
     1621 tggccgtttc gatcgcagcc gttctggccg aactcggccg cgagaacggg aaccttctcg
     1681 ttcaggggcg cgatctcctt gttccagcag gcggtggtcc ggcagcgctg ctcggggtag
     1741 ttgtggaaac cggcgatcag ctggtcgtca tccggtgcgt tggccagcca gccccgcagg
     1801 tcgttggcgt agtcgattcc cgaaaggatt atcggctggg tggccccggc attcctgacc
     1861 acctcggtca gggtggcgag cccggtcgtt cggtaccact tgtggccgct cgtggccgca
     1921 gtgtcaggtt caaccggtgc gtagcagccc ccattcgccc agcaatccca gcccagggtg
     1981 aagaccttcg tgtcgtcggc gttccagcgc gagtggggct cgttgaagag gtcaaagatc
     2041 accgacgggt aatccctgaa gcgggtcgcc accgagctcc agaatgcggc cgaccggttg
//
LOCUS       DAOTIP010000382         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_63150_length_2090_cov_3.737592, whole genome shotgun sequence.
ACCESSION   DAOTIP010000382 DAOTIP010000000
VERSION     DAOTIP010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63150_length_2090_cov_3.737592"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5138_07595"
     CDS             complement(<1..206)
                     /locus_tag="P5138_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60480.1"
                     /translation="MSELAEKDRTTADKRDVAADGSIGMPTATDLELLRSKGRLRYFL
                     ALFGAAFVAAVAYVDPGNFATNVAA"
     gene            complement(309..686)
                     /locus_tag="P5138_07600"
     CDS             complement(309..686)
                     /locus_tag="P5138_07600"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HRV60481.1"
                     /translation="MIELLEDMPEGTIGFRATGEVTADDYRQVLEPAVKKAVEAGEVR
                     MLYVLDGDFKLSAGASLQDTRTGIVAYIHHSEWKKSAIVTDAEWLVKSMHAFGWMVPG
                     ELEVFPVAELDKAKEWLSAGSDS"
     gene            799..1785
                     /locus_tag="P5138_07605"
     CDS             799..1785
                     /locus_tag="P5138_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRV60482.1"
                     /translation="MSENGSTSNGNANGDGEFRTEDEALQVLLDGDIDKVMSGLDRII
                     EAAPSYEKLFMRWQRQHWSTEDYDFTEDARQWNDPDFMTEDERRFMLFGFSQFFLGEE
                     RVTVELLPFAIAAPSHEAKAFLTTQISDEAKHMVFFDRFYREVLGMKADDIGAMLDTQ
                     RPNVNKDWETLFDGILHDCAERLRLDPTDFPALVRGVTVYMIVIEGTLALTGARFIIK
                     SLKERGWLPGFVQGFTAVNRDESRHVGFGVKFLADAIKADPANARIVEDTLKECLPIT
                     PLVFAPTWVDDPYSFETPFYHSTEVFAYAMKALSKKLAAMGLDPAMLAGDPA"
     gene            1875..>2090
                     /locus_tag="P5138_07610"
     CDS             1875..>2090
                     /locus_tag="P5138_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRV60483.1"
                     /translation="MEVLLEEGYAGLTYAKVAVRAGENKSLISYYFGSKQGLVAAVAD
                     RIGELITAEVLTKLRDVDSVGNLNGGLV"
BASE COUNT          372 a          666 c          654 g          398 t
ORIGIN      
        1 gcggcgacgt tggtggcgaa gttgccgggg tcgacgtagg cgaccgcggc cacgaaggcg
       61 gcgccgaaca gggcgaggaa gtacctcagg cgccctttcg accggagcag ttcgaggtcg
      121 gtcgcggtgg gcatcccgat cgacccgtcg gccgcgacgt cacgtttgtc cgccgtggtc
      181 ctgtcctttt ccgcgagctc gctcaacgcc cgagatacta atacctcgga aaagattttg
      241 agtgatgcct aaatatccgg ctcggggccg gtcatcgtcc ttcggcggcg tccgggcccg
      301 ggccggcctc agctatcgga accggcgctg agccactcct tcgccttgtc aagttcggcg
      361 acgggaaaga cctcgagttc ccccggaacc atccagccga aggcatgcat ggacttcacc
      421 agccactcgg cgtcggtgac gatcgcgctc ttcttccatt cggagtggtg gatgtaggcg
      481 acgatgccgg tccgggtgtc ctggagcgag gccccggcac tcagcttgaa atcgccgtcg
      541 agcacgtaga gcatccggac ctctcctgcc tcgactgcct tcttcacggc aggttcgagc
      601 acctggcggt aatcgtcggc cgtcacctcg cccgtggcgc gaaatccgat ggtgccttcg
      661 ggcatgtctt ccagcagttc gatcatctcg gtctccttgg tcgttttccc cctcggtcga
      721 tcttactcag gccggacttg attatttttt ggtcgttcgt ttattatttg ggaatccact
      781 ttgaccttta ggagagagat gagcgagaac ggcagcacga gtaacggaaa tgcaaacgga
      841 gacggcgagt tcaggaccga agacgaagcg cttcaggtcc ttctggacgg tgacatcgac
      901 aaggtgatgt ccggactcga ccggatcatc gaggcggcgc cctcgtacga gaagctgttc
      961 atgcgctggc agcgtcagca ctggtccacc gaggactacg acttcaccga ggacgcccgc
     1021 cagtggaacg acccggactt catgaccgaa gacgaacgcc ggttcatgct cttcggcttt
     1081 tcgcagttct tcctcgggga ggaacgggtc acagtcgagc tgctcccctt tgcgatcgct
     1141 gctccgtcgc atgaagccaa ggccttcctg accacccaga tctccgatga ggccaagcac
     1201 atggtcttct tcgaccgctt ctatcgcgaa gtactgggca tgaaggccga tgacatcggc
     1261 gcgatgctcg atacgcagcg gcccaacgtg aacaaggact gggaaaccct cttcgacggg
     1321 atcctccacg actgtgccga gcggctccgg ctggacccga ccgacttccc ggccctggtc
     1381 cgcggggtga ccgtctacat gatcgtgatc gaaggaacgc tcgccctgac cggcgcccgt
     1441 ttcatcatca agtccttgaa ggaacggggc tggctgcccg gcttcgtcca gggtttcacc
     1501 gcggtcaacc gggacgagtc ccgtcacgtc ggcttcgggg tcaagttcct ggccgacgcg
     1561 atcaaggccg atccggccaa cgccaggatc gtcgaagaca ccctgaagga gtgcctgccg
     1621 atcacgcccc tggtcttcgc tcccacctgg gtcgatgacc cctacagctt cgagacgccc
     1681 ttctaccact cgaccgaggt gttcgcctac gcgatgaagg ccctctcgaa gaagctggcg
     1741 gcaatgggcc tcgatccggc catgctggcc ggcgatccgg cctgaggcga cgcttggaag
     1801 tcgcggcaga aagacagaca cgggggccgg ggcggccctc gtccggggcc cgcgagcgtg
     1861 tactcgacgc cgcgatggag gttctgctcg aggaaggcta cgccgggctc acctacgcca
     1921 aggtggcggt ccgggccggc gagaacaagt ccctcatctc ctattacttc ggctcgaagc
     1981 agggcctcgt ggcggccgtg gccgaccgga tcggcgagct gatcacggct gaggtcctga
     2041 ccaagctgcg ggacgtggac tccgtcggaa acctcaacgg tggtctcgtg
//
LOCUS       DAOTIP010000383         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_63642_length_2078_cov_3.201186, whole genome shotgun sequence.
ACCESSION   DAOTIP010000383 DAOTIP010000000
VERSION     DAOTIP010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63642_length_2078_cov_3.201186"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="P5138_07615"
     CDS             complement(<1..266)
                     /locus_tag="P5138_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60484.1"
                     /translation="MAKVLCVLYEDPVDGYPPDYARDDIPTLTEYPDGQTMPTPSAVD
                     FTPGELLGCVSGELGLRKFLEDAGHELVVTSDKDGEGSAFERELV"
     gene            complement(335..823)
                     /locus_tag="P5138_07620"
     CDS             complement(335..823)
                     /locus_tag="P5138_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRV60485.1"
                     /translation="MSDQERPVVLTGTMNRLNGTEQDLGEYVGNVTLVVNTASKCGLT
                     PQFEALQAIYADRKDQGFTILGFPANDFLEQEPGTADEIAEFCQINYGVEFPMFEKST
                     VVGDEANPLFAELAAQSEQPEWNFAKYLIDREGRLVKKFPAPTTPDDPEVTGAIDAEL
                     GA"
     gene            914..>2078
                     /locus_tag="P5138_07625"
     CDS             914..>2078
                     /locus_tag="P5138_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRV60486.1"
                     /translation="MFRLWFAVAACASLFVLPGLADAEVVMTPMTGNPISDTGQGGVI
                     KAVDLDSDGFDDLVIGSGEAATGGAHIRLAEPDGSWTAKPTLAAGSPVGEITSGDFNG
                     DDKPDLLLNLPDPANPLGASGSFRVFIGAGDGTFNPGQTLTLPGDKVVAVPPTGQSMH
                     LGLINDDDLPDLWVGLTHGQFGIARGEGDGTFSALGVANFPDAPTGDLEGFGPITEGD
                     FNGDGQHDFVLGLSGNGAGSSTEDDSGFFVLYGRGGASYEEPLHVGMLPPYGPVTRLI
                     ARDISGDGNDDLILHTTADGALTSRILIYPGGPEGFGTVPAVLFSIGMPASTVAGADF
                     NSDGKLDLGWVEQLGVVAGNPRPNNLRIATGGSDGAFSLQATAFDLNFAGSKGT"
BASE COUNT          353 a          699 c          659 g          367 t
ORIGIN      
        1 accagttccc gctcgaaggc cgatccttcg ccatccttgt ccgaggtgac tacgagttcg
       61 tgtccggcat cctcgaggaa cttcctcaat ccgagctcgc cggagacgca gccgagcaat
      121 tccccgggcg tgaagtcgac cgccgaaggg gtcggcatgg tctgaccgtc cgggtactcg
      181 gtcagcgtcg gaatgtcatc gcgcgcatag tcgggcgggt acccgtcaac cgggtcttcg
      241 tacagcacgc acagaacctt ggccatctct cctcctgtcc agtcgtggca actggaccca
      301 acctacccga cccccgcggg agggcaaaga ggattcaggc gcccagctcg gcgtcgatcg
      361 ccccggtcac ctcgggatcg tccggggtgg taggcgcagg gaacttcttc accagccgac
      421 cttcgcggtc aatcaggtac ttggcaaagt tccattccgg ctgttcggac tgggcggcga
      481 gctcggcgaa aagaggattc gcttcgtcac cgaccaccgt gctcttctcg aacatcggga
      541 actcgactcc gtagttgatc tggcagaact cggctatctc gtctgccgtg cccggctcct
      601 gctcgaggaa gtcgttggcg gggaagccga gaatggtgaa accctgatcc ttccggtcgg
      661 cgtagatcgc ctgcagggcc tcgaactgcg gggtgaggcc gcacttgctg gcggtgttga
      721 ccacgagggt cacgttgccc acgtactccc ccagatcctg ttcggttccg ttcagccggt
      781 tcatggttcc ggtcaggacg accgggcgct cttgatcgct catgccgcgg atcgtagccg
      841 cagacgaccg ggctttgcgt cagaatccat cctgcttagg gaagccaacc agaagggttg
      901 agggagaaaa ccgatgtttc gtctgtggtt cgccgttgct gcgtgcgcca gcctgttcgt
      961 cctgcccggt ctcgccgacg ccgaggtcgt aatgaccccg atgaccggca atccgatcag
     1021 cgacaccgga cagggcgggg tgatcaaggc tgtcgatctc gactcggatg gtttcgacga
     1081 cctggtcatc ggttccggcg aggcggccac tggcggcgcc cacatccgcc ttgcggagcc
     1141 ggacggcagc tggaccgcga agccgactct cgccgccggg agcccggtcg gcgagatcac
     1201 ttcgggagat ttcaacggcg acgacaagcc ggacctgctg ctcaaccttc ccgacccggc
     1261 gaatcccctc ggggccagcg gatcgttccg ggtcttcatc ggggccgggg acgggacctt
     1321 caaccccggc cagaccctca ccctgcccgg cgacaaggtc gtggccgtgc cgccgaccgg
     1381 tcagtcaatg catctcggac tgatcaacga cgacgacctc cccgacctct gggtcggcct
     1441 gactcacggg cagttcggca tcgcgcgggg agagggtgac ggcactttct ccgccctggg
     1501 cgtagccaac tttccggacg cgccgaccgg cgacctcgaa ggattcgggc cgatcaccga
     1561 aggcgatttc aacggtgacg gccagcacga cttcgtcctc ggcctgagcg gcaacggcgc
     1621 cgggtcttcg accgaggacg actcgggttt cttcgtgctc tacggaaggg gcggagccag
     1681 ctacgaagag ccgctccacg tcggcatgct gcccccctac ggcccggtga cccggctgat
     1741 cgcccgggac ataagcggtg acggaaacga tgacctgatc ctccacacca cggctgatgg
     1801 ggcgctcacc tcccggatcc tgatctaccc gggcgggccc gaaggcttcg gcacggttcc
     1861 cgcggtgctt ttcagcatcg ggatgccggc ctcgaccgtg gccggcgccg acttcaacag
     1921 tgacggaaag ctggacctcg gctgggtcga acagctcggc gtggtcgccg gcaaccccag
     1981 acccaacaac ctcaggatcg cgaccggcgg cagcgacggt gccttttcgc ttcaggcgac
     2041 cgccttcgac ctcaatttcg caggcagcaa gggcaccg
//
LOCUS       DAOTIP010000384         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_63843_length_2073_cov_3.625372, whole genome shotgun sequence.
ACCESSION   DAOTIP010000384 DAOTIP010000000
VERSION     DAOTIP010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63843_length_2073_cov_3.625372"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..129)
                     /locus_tag="P5138_07630"
     CDS             complement(7..129)
                     /locus_tag="P5138_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60487.1"
                     /translation="MLQGLDCVHPAAAYASAAGFTLSFSPDQLVWIASTAGVIC"
     gene            146..1099
                     /gene="pstC"
                     /locus_tag="P5138_07635"
     CDS             146..1099
                     /gene="pstC"
                     /locus_tag="P5138_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRV60488.1"
                     /translation="MSAGTVSPGNSSTPGKVHRRLGDRAFSGLATAAGVTILVVLAGV
                     AAFLVSEAWPAITAPVADLPDGKNLVHYIWPLVFGTLLSSTIAIIIAVPMAIAVALFT
                     SHIAPKPIAMSVSYLVDLLAAIPSIIYGIWGIAVLGPASVPATQWLADHFGFIPLFDG
                     PASTTGRTMLVAGIVLALMILPIISAVAREVFQQTPKGLQESALALGATRWEMIRMVV
                     MPFGRSAVVAGSMLGLGRALGETMAVTIILSVSGVISFDLISSENPSTIAANIALQFP
                     ESSGIDVNVLVTTGLVLFAITLAVNMAARTIISRREAAGGR"
     gene            1089..>2073
                     /gene="pstA"
                     /locus_tag="P5138_07640"
     CDS             1089..>2073
                     /gene="pstA"
                     /locus_tag="P5138_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRV60489.1"
                     /translation="MAGSTVSVPLGDGSGVLPRRAALIVFGVSALASAILSLALGLSP
                     FVWLIFTGLFSLLTITVWSLAVEGRRRALDRVMTVVIVDIFILALVPLGSLIYTVVKE
                     GTARFDGEFLTSTMRGVLADGGGALHAAMGTFIITAFATLAAVPVGVMAAIYLVEYGK
                     GRLKRAITFFVDVMMGIPSIVAGLFAFALFSIFLGPGIRLGIMGSVALMVLMIPIVIR
                     SAEEMLRVVPDSLREASFALGVPKWITVLRVVLPTALAGIVTGVMLSIARVIGETAPL
                     LITTGSVDSMNLDPFNGRMANLPVYVYEQYKFPGVPPEAYIERAWAGALLLI"
BASE COUNT          305 a          707 c          639 g          422 t
ORIGIN      
        1 gctgcgtcag cagatcaccc ccgccgtgga ggcgatccag accagctgat ccgggctgaa
       61 cgacagcgtg aaccccgcgg cggaggcata ggccgccgcg gggtgaacgc agtcaagacc
      121 ctgaagcatc aggcaggaga atcatttgag cgccggcacc gtctcaccag gtaattcatc
      181 aactccaggc aaggtccacc gcagactcgg tgaccgagcc ttttccgggc tggcaaccgc
      241 cgccggagtc acgatcctgg tcgtcctggc cggggttgcc gcatttctgg tcagcgaggc
      301 atggcccgcg atcaccgcgc cggtcgcgga tcttcccgac ggcaagaacc tcgtccacta
      361 catctggccg ctcgtcttcg gaacccttct ttcctcgacc atcgccatca tcatcgcggt
      421 gcccatggcc atcgccgtgg ccctcttcac cagccacatc gcccccaagc cgatcgcgat
      481 gtcggtttcc tacctggtcg acctgctcgc tgcgatcccc agcatcatct acgggatctg
      541 gggcatcgcc gtgttgggac cggcctcggt gcccgccacc cagtggctgg ccgaccactt
      601 cggcttcatc cccctcttcg acggccccgc gtccacaacc ggccgcacca tgctggtcgc
      661 cgggatcgtg ctggccctga tgatcctgcc gatcatcagc gcggtcgccc gcgaggtctt
      721 ccagcagacg cccaagggcc ttcaggaaag cgccctggcg ctcggggcca cccgctggga
      781 aatgatccgg atggtggtca tgcccttcgg tcgctcggcg gtggtcgccg gctcgatgct
      841 cggcctcggc cgggcgctcg gcgagaccat ggccgtgacg atcatccttt cggtctcggg
      901 agtcatcagc ttcgacctga tcagttccga gaatccatcc acgatcgcgg ccaacatcgc
      961 gcttcagttc ccggagtcct ccgggatcga cgtcaacgtc ctggtcacca cgggtctggt
     1021 cctgttcgcg atcacgctcg cggtcaacat ggccgcccgg acgatcatca gcagaaggga
     1081 ggccgccggt ggccggtagc accgtttcgg taccgcttgg cgacggttcg ggggttctgc
     1141 cccgccgggc cgcgctgatc gtcttcggcg tctcggcgct cgcctcggcg atcctcagcc
     1201 tcgcgctggg tctcagcccg ttcgtctggc tgatcttcac cgggctgttc agtctgctca
     1261 cgatcaccgt ctggtcgctg gcggtggaag gccgtcggcg ggccctggac cgtgtgatga
     1321 ccgtggtcat cgtcgacata ttcatcctcg ccctggtgcc ccttggctcc ctgatttaca
     1381 cggtggtcaa ggaaggtacg gcgaggttcg acggcgagtt cctgaccagc accatgcgcg
     1441 gtgtcctggc cgatgggggt ggggccctcc atgctgcaat gggaaccttc atcatcaccg
     1501 ccttcgccac cctcgccgcc gtgccggtcg gggtcatggc cgcgatctac ctggtcgagt
     1561 acggcaaggg ccggctcaag cgggcgatca ccttcttcgt tgacgtgatg atgggcatcc
     1621 cctcgatcgt cgccggtctc ttcgcctttg ctctcttctc gatcttcctc ggccccggga
     1681 tccgtcttgg aatcatgggt tcggtggcat tgatggtgct gatgattccg atcgtgatca
     1741 ggtccgcgga agaaatgctc agggtggtcc cggattcgct gcgggaagct tccttcgcgc
     1801 tcggcgtacc gaagtggatc accgtgctcc gggtcgtcct gccgaccgct ctcgcgggga
     1861 tcgtgaccgg tgtgatgctg tccatcgccc gcgtgatcgg cgagaccgca ccgttgctga
     1921 tcaccaccgg ttcggtcgat tcgatgaacc tcgatccgtt caacggccgg atggcaaacc
     1981 tgccggtcta cgtttacgag cagtacaagt tcccgggtgt tcctcccgag gcctacattg
     2041 aaagggcctg ggcgggagcg ctgctgttga tca
//
LOCUS       DAOTIP010000385         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_63846_length_2073_cov_3.312190, whole genome shotgun sequence.
ACCESSION   DAOTIP010000385 DAOTIP010000000
VERSION     DAOTIP010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63846_length_2073_cov_3.312190"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="P5138_07645"
     CDS             complement(<1..1110)
                     /locus_tag="P5138_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRV60490.1"
                     /translation="MSREKWVTLIVASAATAILLLDVTIVYVALPSIRADLGASFNQA
                     QWVIDAYTVVMAATLLACGSLADRIGRRKIFAGGLIVFTTCSLVCGVAGSPLVLDLSR
                     GVQGLGAAAMYAASLALLANEFQGSQRGLAFGVWGAVTGAALAVGPLIGGLVIDGLSW
                     RWIFLINVPIGLLTLLGTWASVRESKEPKESRFDLAGTVLFAASTFLLVLALIRGNDD
                     GWGSTFIVATFLVAAGLAAAFITVELRRSEPMLPLGFFRSKPFTGTAIVSFAQSIAIY
                     PLLLFLAIYLQEGLGFGPTEAGLRLLPMTVTIFLVAPFAGRLTSRTTIRLPLASGLFL
                     LAVSLLLIYGIDAENGWTRLLPGMLVGGVSIGLISP"
     gene            1193..1402
                     /locus_tag="P5138_07650"
     CDS             1193..1402
                     /locus_tag="P5138_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60491.1"
                     /translation="MGDHLIPILLVLWQTLLCAGFAALYWLGIIHDPRNLLFSGLLSH
                     IGAIMVFPVGWAVLVWKTMTGDLNG"
     gene            complement(1476..1548)
                     /locus_tag="P5138_07655"
     tRNA            complement(1476..1548)
                     /locus_tag="P5138_07655"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1513..1515),aa:Lys,seq:ctt)
     gene            complement(1651..>2073)
                     /locus_tag="P5138_07660"
     CDS             complement(1651..>2073)
                     /locus_tag="P5138_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60492.1"
                     /translation="NVLFTLSQGSTVAQFQSNIKDAAKAQQFVADSAVKEQKNGGASA
                     ATQLTTLAIYQLYAQDYAAAEKTLNQALAATSDSAEKKQIKQTYNSSEKDAKRVGKLI
                     DKALKQAKKDGGKSLENPLGSLGSDTSITGASGATTTP"
BASE COUNT          372 a          672 c          682 g          347 t
ORIGIN      
        1 cggactgatc aggccgatcg agacaccgcc gaccagcatg ccgggcagca ggcgggtcca
       61 gccgttctcc gcatcgatgc cgtagatcaa gagcagactc acggcgagca ggaagaggcc
      121 ggaggcgagc ggcagcctga tcgtcgtcct ggaggtgagc cgtccggcga agggggcaac
      181 gaggaagatg gtgaccgtca tcggcagcag cctgagcccg gcctcggtcg ggccgaaccc
      241 gaggccttcc tgcaggtaga tcgcgaggaa caggagcagc gggtaaatcg cgatcgactg
      301 cgcgaaggaa acgatcgcgg tgccggtgaa cggctttgaa cggaagaagc cgagtggaag
      361 catcggctcg ctccgccgga gttcgaccgt gatgaaggcc gctgccagcc cggccgcgac
      421 aagaaaggtc gcgacgatga aggtgctgcc ccagccgtcg tcgtttccgc ggatcagggc
      481 gagcacaagc aggaaggtcg acgccgcgaa caggaccgtg cccgcgaggt cgaagcggga
      541 ctccttcggt tccttcgatt cccggaccga ggcccaggtg ccaagcaggg tcaggagccc
      601 gatcgggacg ttgatcagga agatccaccg ccagctcaat ccgtcgatca ccagcccgcc
      661 gatcagcgga ccgaccgcca gggcggcacc ggtcaccgcc ccccacactc caaaggcaag
      721 tccgcgctgg ctgccctgga actcgttggc gagcagggcg agtgacgccg catacatcgc
      781 cgccgcgccg agcccctgca ctccgcggga caggtcgagg accagcgggc tgccggccac
      841 cccgcagacc agcgaacagg ttgtgaagac gatcagtccg ccggcgaaaa tcttccgccg
      901 gccgatccgg tcggccagtg agccgcaggc gagcagggtc gcggccatga ccacggtgta
      961 ggcatcgatc acccactggg cctggttgaa gctcgccccg aggtccgccc ggatcgacgg
     1021 cagcgccacg tagacgatcg tcacgtccag cagcaggatc gccgtcgccg ccgacgcgac
     1081 aatcaatgtc acccacttct cccggctcat ctgcccgcga accctagagc ggaaagcgga
     1141 ggcgatgttc gtcctgtcag atccgaagcc cggcgaccga ccctgtcggc atatgggtga
     1201 tcatctgatc ccgatccttc tcgtcctctg gcagaccctg ctctgcgccg gtttcgccgc
     1261 gctctactgg ctggggataa tccacgaccc gcggaacctc ctcttctccg gcctgctctc
     1321 tcacatcgga gcgatcatgg tcttcccggt cggctgggcc gtcctcgtct ggaagacgat
     1381 gaccggtgac ctcaacggct gagcgagcac tcctgagacc gcgagtcgtg gcagcggcca
     1441 ggcgggcatg cgtcaatccc gtggaactcc ccgagtgggc cgtgaaggga tcgaaccttc
     1501 gaccttgaga ttaagagtct cctgctctac cagctgagct aacggcccgg aacgcggctc
     1561 attcgaatta agccggatcg gaagtataga gcgggcggac cattttcgtg gcccgcccgc
     1621 cctttggacg agcttcgtga aacgggtcga tcagggcgtg gtggttgcgc cggaggcgcc
     1681 ggtgatcgag gtgtcggagc cgaggcttcc gagcggattc tcgagcgact tgccgccgtc
     1741 cttcttggcc tgcttgagcg ccttgtcgat cagcttgccg acccgcttcg catccttctc
     1801 ggaagagttg taggtctgct tgatctgctt cttctcggcc gaatcgctgg tcgcggcaag
     1861 agcctggttc agggtcttct cggcggccgc gtaatcctgg gcgtaaagct ggtagatcgc
     1921 cagggtggtg agctgggtgg ccgcgctcgc cccgccgttc ttctgttcct tcacggcact
     1981 gtcggcgacg aactgctggg ccttggccgc atccttgatg ttgctctgga actgggccac
     2041 cgtcgaaccc tgcgacaggg tgaagaggac gtt
//
LOCUS       DAOTIP010000386         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64111_length_2067_cov_3.604374, whole genome shotgun sequence.
ACCESSION   DAOTIP010000386 DAOTIP010000000
VERSION     DAOTIP010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64111_length_2067_cov_3.604374"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..399
                     /locus_tag="P5138_07665"
     CDS             25..399
                     /locus_tag="P5138_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60493.1"
                     /translation="MAQTKKKRTRKKRGTQAGNVDTSRRSRPRSRAEAKNQARSGGGG
                     RSRSMQKGDNPPTWRGAAFRGLIASGIFVLLLLLLFGRPLTSSLIIGLVMLVFYIPAG
                     FYMDLYLWRRRERQRIRAKSKG"
     gene            402..1049
                     /locus_tag="P5138_07670"
     CDS             402..1049
                     /locus_tag="P5138_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV60494.1"
                     /translation="MAGLEVIGTTVGPVAENSYLIFREGTGKALLVDPGDEADRLMQP
                     LAERDLELEAILLTHCHFDHIGAVAPIARATGAPVYCPLIEVPILADVMAYVPWPGIG
                     PFESYDADHTVEGGEHLSLAGMEIDVLFTPGHSPGHVTYSIPEAGLLLSGDVLFQGSV
                     GRVDLPGGDWDTLLESIRMLITNFPEETQVLPGHMGVTTLGAELATNPFLTEIPR"
     gene            1057..>2067
                     /gene="hisS"
                     /locus_tag="P5138_07675"
     CDS             1057..>2067
                     /gene="hisS"
                     /locus_tag="P5138_07675"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRV60495.1"
                     /translation="MAEKFKAPRGTFDVLPEQAAQRERLLQAAREIFGLAGYRLIATP
                     VFEDTALFERGVGKSTDIVRKEMFTFEDKGERSLTLRPEGTASICRAYIEHGMHKRPQ
                     PVKLFYAGPFFRHERPQAGRYRQFNQIGAEAIGSDSPLVDVEVITLISDLIEELGVPG
                     VELRLGSLGSLDARATYLEELKSHLRSREDELSGDVRERIEINPLRAFDSDHEGTQAV
                     MADAPQLLDFLSEEDADHFEQVKSFLGTASVEFTVDPTLVRGLDYYTRTIFSFVCEGL
                     GAQSEIGGGGRYDGLIEQLGGPATPAVGWAAGIERILLALDEEVTSAGMDLFVVVPDP
                     ELT"
BASE COUNT          350 a          686 c          681 g          350 t
ORIGIN      
        1 gccgttctcc gataggtttc gaccgtggca cagaccaaga agaaacgcac gagaaagaag
       61 cgcggcacgc aggccggcaa cgtcgatacc tcccgtcgct cccgcccgcg cagccgggcc
      121 gaagcgaaga atcaggcacg gtccggaggc ggtggccgga gccgttcgat gcagaagggc
      181 gacaatccgc cgacctggcg gggcgccgcg ttccggggcc tgatcgcttc gggcatcttc
      241 gtcctcctgc tgctcctgct cttcggccgg ccgctcacca gttcgctgat catcggcctc
      301 gtgatgctgg tcttctacat cccggccggc ttctacatgg acctttacct ctggcggcga
      361 cgcgagcgcc agcggatccg ggccaagagc aagggctagg cgtggccggg ctcgaggtca
      421 tcggaaccac ggtcgggccg gttgcggaaa actcgtacct gatcttcagg gagggaaccg
      481 gaaaggcgtt gctggtcgat cccggtgacg aggccgaccg gctgatgcag cccctggccg
      541 agcgggacct cgaactcgag gccatcctgc tcactcattg ccacttcgat cacatcgggg
      601 cggtcgcccc gatcgccagg gcgaccgggg ctccggtcta ctgccccctg atcgaggtgc
      661 cgattcttgc cgacgtgatg gcctacgtgc cgtggcccgg tatcggcccc ttcgaaagtt
      721 acgacgccga tcacacggtt gagggtggcg agcacctctc gctggccggc atggagatcg
      781 acgtgctgtt cacccccggt cacagccccg gccacgtcac ctactcgatt cccgaggccg
      841 ggttgctcct atccggggac gtcctcttcc agggctccgt gggaagggtc gacctgcccg
      901 gcggcgactg ggacaccctg ctcgagtcga tccgcatgct gatcaccaat ttccccgagg
      961 agacgcaggt cctgcccggc cacatgggag ttaccaccct cggggccgag ctggcgacca
     1021 atccgttcct gaccgagatc ccgaggtgag gacctgatgg ccgagaagtt caaggcgccg
     1081 cgaggcacct tcgacgtcct tcccgagcag gctgcccagc gggagcgtct gctgcaggcg
     1141 gcccgggaga tcttcggcct ggccggctac cggctgatcg cgaccccggt cttcgaggac
     1201 acggcgctgt tcgagcgggg cgtcggcaag tcgaccgaca tcgtccgcaa ggagatgttc
     1261 accttcgagg acaagggcga gaggtcgctg accctgcggc ccgagggaac ggcctcgatc
     1321 tgccgcgcct acatcgaaca cggcatgcac aagcggccgc agccggtgaa gctcttctat
     1381 gcgggcccgt ttttccgcca cgagcggccc caggccgggc gataccggca gttcaaccag
     1441 atcggtgcgg aggcgatcgg gtccgactcg cccctggtcg acgtcgaggt aatcaccctg
     1501 atcagcgact tgatcgagga actcggggtg ccgggagtgg aattgcggct cggaagcctc
     1561 ggctcgctcg atgccagggc tacgtatctc gaagaattga agtcacatct cagatcacgg
     1621 gaggacgagc tttccgggga tgtacgcgag cgcatcgaga tcaatccgct ccgggccttc
     1681 gattccgacc acgagggcac acaggcagtg atggccgacg ccccgcaact gctcgatttc
     1741 ctcagcgagg aagacgccga tcacttcgag caggtcaagt ccttcctcgg gactgcatcg
     1801 gtcgagttca cagtcgaccc gaccctggtc cgcggtctcg actactacac ccgcacgatt
     1861 ttcagcttcg tctgcgaagg gcttggcgcc caatccgaga tcggcggtgg tggtcgctac
     1921 gacggcctga tcgagcagct cggcgggccg gcgacgccgg cggtcggctg ggcggccggc
     1981 atcgagagga tcctgctcgc cctcgacgag gaagtcacct cggccgggat ggacctgttc
     2041 gtggtggtgc ccgatccgga gctcacg
//
LOCUS       DAOTIP010000387         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64323_length_2063_cov_2.186753, whole genome shotgun sequence.
ACCESSION   DAOTIP010000387 DAOTIP010000000
VERSION     DAOTIP010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64323_length_2063_cov_2.186753"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      <1..103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            113..1273
                     /gene="thiO"
                     /locus_tag="P5138_07680"
     CDS             113..1273
                     /gene="thiO"
                     /locus_tag="P5138_07680"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HRV60496.1"
                     /translation="MTRAEAKDRSDVIVVGAGLIGLSVAWKLALDGASVTLLEREKPG
                     AGATGVAAGMLAPIGELDFGEPELLELNLRSRRLYPDFVAGVEAETGMDTGYRHTGAL
                     HVALDRDEAGELRRIMELQQSHGLDSKWLGPMAARDLEPGVSPSLAGAVLVEDDAVVD
                     PRALVEALRSGLEARGTRIVPAEVSGLLRDSSGKVTGVLTGDGEFTARVVVAAPGAEA
                     GVAEWLPEEVRPPVRPVKGQVVELRGDTDAPACERILGSERVYIAPRPDGRLILGATV
                     EEMGFDRRVTGGGVHELLREAYRLLPDVAEMEFIGAIAGLRPGTPDNVPVIGPAGIEG
                     LILAAGHYRNGILLAPVTAEAVVDFVQGRQPEGLTAASPARFENQAVGGGER"
     gene            1274..>2063
                     /gene="thiS"
                     /locus_tag="P5138_07685"
     CDS             1274..>2063
                     /gene="thiS"
                     /locus_tag="P5138_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRV60497.1"
                     /translation="MRIELNGEQTEVADGATVSELIGLVGGGRIPPDRRGVAVALDGE
                     VIPRSAWESTAPAEGASIEVVAAIQGGSGESGEEKWELGDRSWTSRLIAGSGGFRSLE
                     AMESALALSGTEIITVALRRLDPDAQGSVMDVIEKLGLFILPNTAGCYTARDAVRTAK
                     LAREAFGTDWVKLEVIGDDRTLLPDAPELLRAAEDLVGDGFTVLPYTNDDPILAARLE
                     AAGCAAVMPLGGPIGSGAGIRNPYNISIIVENAKVPVILDAGIGT"
BASE COUNT          338 a          659 c          754 g          312 t
ORIGIN      
        1 gcgggagccc gttgttccgg gctgagaggg tggcgacgaa gccgccgacc gcctgaacct
       61 gatccgggta attccggcgg cagggaaagg gcaacggctc cacagggaag aaatgacgcg
      121 agccgaagcg aaagacagaa gcgatgtgat cgtggtcggg gccggcctga tcggtctgtc
      181 cgttgcctgg aagctcgccc tcgatggtgc gtcggtcacg ctgcttgaac gcgagaaacc
      241 aggtgcgggc gcgactggcg tcgccgccgg aatgctcgcc ccgatcggcg agctcgactt
      301 cggcgagccc gaactgctgg agctgaacct gcgctcgcgg cggctctacc cggacttcgt
      361 cgctggggtc gaagccgaaa ccggaatgga caccggctac cggcacaccg gggcgctcca
      421 tgtcgcgctg gaccgcgacg aggcaggcga gctgaggcga atcatggagc tgcagcagag
      481 tcacggcctg gattcgaagt ggttggggcc gatggcggca agggacctgg aacccggggt
      541 cagcccgtcc ctggccggag cggtgctggt cgaggacgat gccgtggttg acccgcgggc
      601 gctggtcgaa gcgctcagat cagggctgga agcgcgggga acgcggatcg tgcccgcaga
      661 ggtttccggt ctcctccgcg actcctccgg aaaggtcacc ggtgtcctga ccggcgacgg
      721 cgagttcacc gcccgggtcg tggtcgccgc gcccggtgcc gaggccggtg ttgccgaatg
      781 gctgccggaa gaagtacgcc ctccggtcag gccggtcaag ggccaggtgg tcgaactccg
      841 cggcgacacc gatgcccccg cctgcgagcg gatcctcggc tcggagaggg tgtacatcgc
      901 gccaaggccg gacggccggc tgatcctcgg ggcgacggtc gaagagatgg gtttcgacag
      961 acgggtgacc ggtggcggag ttcacgaact gcttcgggag gcctaccggc tgctgccgga
     1021 tgtcgccgag atggaattca tcggcgcgat cgccggcctt cgtccgggca ccccggacaa
     1081 cgtgccggtg attggtcccg ccggaatcga agggctgatc cttgccgccg gccactaccg
     1141 gaacggcatc ctgctcgccc cggtcaccgc cgaggccgtt gtggatttcg tccagggcag
     1201 gcaaccggaa ggcctgacgg cggcgagccc ggcccggttt gagaaccagg ccgtcggagg
     1261 gggagaaagg tagatgcgga tcgaactgaa cggcgagcag accgaggtcg cggacggggc
     1321 gaccgtctcc gaactgatcg gcctggtcgg gggcgggcga atcccgccgg atcgccgcgg
     1381 ggtcgcggtc gcgctcgacg gtgaggtcat tccccgctcg gcctgggaat caacggctcc
     1441 cgccgagggc gcttcgatcg aggtcgtggc ggctattcag ggcggctccg gggagtctgg
     1501 agaggaaaag tgggaactcg gcgaccgaag ctggacttcc cgcctgatcg ccggaagcgg
     1561 cggattccgc tcgctcgaag caatggaaag cgcccttgcc ctgtccggga ccgagatcat
     1621 cacggtcgcg ctccggcggc tcgaccctga cgcccagggt tcggtcatgg acgtgatcga
     1681 aaagctcggc ctgttcatcc tgccgaatac ggccggctgt tacaccgccc gtgacgcggt
     1741 ccgcaccgcg aaactcgccc gcgaggcctt cggcaccgac tgggtgaagc tggaagtgat
     1801 cggcgacgac cgcacgctcc tccccgatgc gcccgagttg cttcgggccg ccgaggatct
     1861 ggttggtgac ggtttcaccg tgcttcccta caccaacgac gacccgatcc tcgccgcccg
     1921 cctggaagcg gccggctgcg cggcggtgat gcccctcggc ggcccgatcg gttccggggc
     1981 gggcatccgc aatccgtaca acatctcgat catcgtcgag aacgcgaagg tgccggtgat
     2041 cctcgatgcc gggatcggca ccg
//
LOCUS       DAOTIP010000388         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64462_length_2059_cov_3.653194, whole genome shotgun sequence.
ACCESSION   DAOTIP010000388 DAOTIP010000000
VERSION     DAOTIP010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64462_length_2059_cov_3.653194"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="P5138_07690"
     CDS             complement(<1..256)
                     /locus_tag="P5138_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60498.1"
                     /translation="MGRFRTALATISVGALLATGHAASAESATPINDEPATTAGVGPI
                     GPTGSTGSTGSTGTTGSTGTTGSGNSGKNDGNGNSGNGGKN"
     gene            complement(266..1084)
                     /locus_tag="P5138_07695"
     CDS             complement(266..1084)
                     /locus_tag="P5138_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60499.1"
                     /translation="MHHETNENWQDYGKPPGRLSWPVRRLGWFLEKHLLWPIGDSFRR
                     FTNAFRYRSPLAYIGATLMVAVTAGAIAAAVYFYNQSKDNGSDPVVATTAIPAETVVP
                     STPTPTTAPTTVEDTTPNSGNADDTLQGVVPSFTASSKKKSAAAKSSGSTQKLPGTVV
                     RPAPVPKSPPLKVAHSFATTFVSYEIGGKGAAKKFSATATPELAKELRQNPPKLPSTG
                     QIPKATVVNVVAGKKTGDKLEASVSLMRSGAASELRLTLIRKKGKGWLVSEVRG"
     gene            complement(1289..>2059)
                     /locus_tag="P5138_07700"
     CDS             complement(1289..>2059)
                     /locus_tag="P5138_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV60500.1"
                     /translation="SFLFLGPTGVGKTELAKSLAEFMFDTDQAIVRLDMSEYMEKHTV
                     ARLIGAPPGYVGYEEGGQLTEAVRRRPYAVVLLDEIEKAHPDVFNTLLQLMDDGRLTD
                     GQGRTVSFTNTVLIMTSNVGSQEIAGENVDERIRERVMEVLETTFKPEFLNRIDDTVI
                     FHRLSKEDIGRIVEIQVEGLTRRVAERDIEIELTDDARTLLGNLGYDPTYGARPLKRV
                     VQKQLVDKLALKILEGEFSEGDSVLIDASDGELAFTKK"
BASE COUNT          304 a          622 c          703 g          430 t
ORIGIN      
        1 ggttcttgcc tccgttaccc gagttgccat tgccgtcgtt cttgccgctg tttccggaac
       61 cggttgttcc ggtggagccg gtggtgcccg tcgatcccgt agaaccggtg gacccggtgg
      121 ggccgatcgg tccgactccg gcagtcgtcg ccggctcgtc gttgatcggc gtcgcgcttt
      181 cggcgctcgc tgcatggccg gtggcgagca gggctccgac cgagatcgtc gccagggctg
      241 ttctgaacct ccccatgctt ctttgttacc cccggacctc gctgaccagc catcccttgc
      301 ccttcttgcg gatcagggtg agccggagtt ccgaggctgc gccggagcgc atcagagaga
      361 cactggcctc gagcttgtcg ccggtcttct tgccggcaac gacgttgact acggtggcct
      421 tcgggatctg gccggtcgag gggagcttcg gcgggttctg cctgagctcc ttggccagtt
      481 caggggtggc ggtggcggag aatttctttg ccgcgccctt gccgccgatc tcgtagctga
      541 cgaaggtggt ggcgaagctg tgggcgacct tcagcggcgg tgacttgggt acgggcgcag
      601 gacgaacaac ggtgccgggg agcttctgcg tcgaaccgga ggacttggcg gcggccgact
      661 tcttcttgct cgaagcggtg aagctgggaa ccacaccctg gagggtgtcg tcggcgttac
      721 ccgagttcgg ggtggtgtcc tcgacggtgg tcggagcggt cgtcggggtc ggggtcgaag
      781 gcacgacggt ctcggccggg atcgccgtgg tggccacaac cgggtcgctg ccgttgtcct
      841 tggactggtt gtagaagtac accgcggcgg cgatcgcacc ggcggtgacg gcaaccatca
      901 gggtggctcc gatgtaggcg agcgggctgc ggtagcggaa ggcgttggtg aagcgtcgga
      961 aggaatcgcc gatcggccag agaaggtgtt tctccaggaa ccagccgagt ctccggacag
     1021 gccagctcag cctgccggga ggcttcccgt agtcctgcca attttcgttg gtttcgtgat
     1081 gcatcgaatc gcgagccgtt ttccgcttct gatctggatc tccccgtacc cgtttcgggc
     1141 gtcacagggt agtacgggaa cccgaaaggg gaagtaaagg agcggttaag cgggtgcgaa
     1201 actgcagtca ggcgaaccag cttcaaacgc cggtgggaag ccgttttcgg cttcccaccc
     1261 aagcattccc gagcctcccc ggggactgtt atttctttgt gaaagcgagt tccccgtcgg
     1321 atgcgtcgat caggaccgag tcgccttcgc tgaattcgcc ttcgaggatc ttcagcgcca
     1381 gcttgtcgac cagctgcttc tggaccaccc gcttcagcgg cctcgcgccg taggtcgggt
     1441 cgtatccgag gttcccgagc agggtcctcg catcgtcggt cagttcgatc tcgatgtcgc
     1501 gctcggcgac ccgcctggtc aggccctcga cctggatctc gacgatccgg ccgatgtctt
     1561 ccttggacag gcgatggaag atcacggtgt catcgatccg gttgaggaac tcgggcttga
     1621 aggtcgtttc caggacctcc atcacccgct cgcggatccg ctcgtcgacg ttctcgccgg
     1681 cgatctcctg ggagccgacg ttcgaggtca tgatcagaac cgtgttggtg aagctgaccg
     1741 tgcggccctg gccgtcggtc aggcgcccgt catccatcag ctggagcagc gtgttgaaga
     1801 cgtcggggtg ggccttctcg atctcgtcga gcagcaccac cgcgtagggt cggcggcgga
     1861 ccgcttcggt cagctggccg ccctcttcgt agccgacgta tccggggggc gccccgatca
     1921 gccttgcgac ggtgtgcttc tccatgtatt cggacatgtc gagccgcacg atcgcctggt
     1981 cggtgtcgaa catgaactcg gccagcgact tggccagctc ggtcttgccg acaccggtcg
     2041 gtccgaggaa gaggaagga
//
LOCUS       DAOTIP010000389         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64478_length_2059_cov_2.964571, whole genome shotgun sequence.
ACCESSION   DAOTIP010000389 DAOTIP010000000
VERSION     DAOTIP010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64478_length_2059_cov_2.964571"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="P5138_07705"
     CDS             complement(<1..99)
                     /locus_tag="P5138_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016365427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRV60501.1"
                     /translation="MSVKVAINGFGRIGRNVFRAAHEAGADLDIVAI"
     gene            complement(504..866)
                     /locus_tag="P5138_07710"
     CDS             complement(504..866)
                     /locus_tag="P5138_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60502.1"
                     /translation="MIHHIVLEVSDLPRACSFYDALLSPLGWRRHVNETNAVGYGISR
                     PILFITDRTGVRAEGTMVSLGAPGIAAVKAAWEGGTNSGGSNVAQPGETSAHGSGSYS
                     AFLRDPDGHDIEITVSSD"
     gene            complement(934..1704)
                     /locus_tag="P5138_07715"
     CDS             complement(934..1704)
                     /locus_tag="P5138_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60503.1"
                     /translation="MRQVSLALHEFRFAQKVFWRNPASVFFTALLPVLFLLIFATIFG
                     DDTIKDLGGVKITTYYIPAIITLAVISATMVNLAMNVTIAREEGLLKRGRGTPLPPWV
                     FIAGRVGNAIVISAIMVVVVTLIGKLLYDAPIPWDRIPAVIVTLVVGAASFCCLGLAI
                     TVIIPSREAASAITNAITLPLYFLSGIFIPENEIPDGVLSFANHFPVRDFFQAFFSAY
                     SPKTVGSGFEWGELAVVALWGLAGLIIALRYFRWTPRS"
     gene            complement(1701..>2059)
                     /locus_tag="P5138_07720"
     CDS             complement(1701..>2059)
                     /locus_tag="P5138_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60504.1"
                     /translation="THYMEEAQYLADRVAIMSEGEIVALDTPENLGDRRRLPTTITFV
                     LPPGTDLSDLEGVGQTRSDRTAEDGRLEISVSTHEAVSDLNRITAWAIAQGIDIQQLE
                     VRRPSLEDVYLELTDA"
BASE COUNT          378 a          643 c          692 g          346 t
ORIGIN      
        1 gatcgcgacg atgtccagat cagcacccgc ctcgtgcgct gcccggaaaa cgttccgccc
       61 gattcgaccg aaaccgttga ttgccacctt gacgctcatt cgctaccgcc tcctgaataa
      121 ctgggttgcc ttggggggaa atgggactac aagtaaattc tacggccctc caaactctga
      181 tgccgcccgg tcccctacct gccctggcgg ttacgccgcg gggccgggcc gtgaggccgc
      241 ggggccggca tgcatccccg ggacctcgta ctcgctgcgc tcgtacacgg gagtttcccg
      301 gggacgcatg accgacccca cggccacccg aacgcggacc cggcaacacc gtgacgcagg
      361 ttcgggcacc ccgccggtca caaacctgcg gcaccacccg cgcggcatcg gcccgtggaa
      421 ggcgaaacac atttcctgca gacgacgcac aaaacttgtt acaccgtgcg ctgccgagcc
      481 cgaacttctg gcgccgcgcg agcctagtca gaggagacgg tgatctctat gtcgtgcccg
      541 tcgggatcgc ggaggaaggc cgagtaggaa cctgatccgt gggcgctggt ctctcccggc
      601 tgggcgacgt ttgagccgcc ggaattcgtg ccgccctccc aggccgcctt gaccgcggcg
      661 attcccgggg caccgaggct gaccattgtc ccttcggccc ggacaccggt ccggtcggtg
      721 atgaagagga tcggccggga gatgccgtat ccgactgcgt tggtctcgtt cacgtgccga
      781 cgccagccga gtggcgagag cagcgcgtcg tagaaggagc aggcgcgggg aaggtctgag
      841 acttcgagaa cgatgtggtg aatcacttgc gtcaggctag tggagatgca acaaatccgg
      901 aaggtgaccg gatgctgtat gggtccgtcg ggactagctg cgcggggtcc agcggaagta
      961 gcgcagcgcg atgatcagac cggccagtcc ccagagcgcg actaccgcca gctcgcccca
     1021 ctcgaagccg gaaccgacgg tcttcgggct gtaggcactg aagaaggcct ggaagaagtc
     1081 ccgtaccggg aaatggttcg cgaaagacag gacgccgtcg gggatctcgt tttcggggat
     1141 gaagatcccg gaaaggaaat aaagcggcag ggtgattgcg ttggtgatgg ccgaggccgc
     1201 ctcccgcgag ggaatgatca cggtgatcgc caggccgagg caacagaacg atgccgcgcc
     1261 gaccaccagc gtgacgatca cggcggggat gcggtcccag gggatcggtg cgtcgtagag
     1321 cagcttgccg atcagggtga ccacgacgac catgatggcc gagatcacaa tcgcgtttcc
     1381 gacccggccg gcgatgaaca cccagggcgg caagggggtg ccccggcctc ttttcaggag
     1441 cccctcttcc ctggcgatcg tcacgttcat cgccaggttg accatggtgg ccgagatcac
     1501 cgcgagggtg atgatggccg ggatgtaata ggtggtgatc ttgaccccgc cgaggtcctt
     1561 gatcgtgtcg tcgccgaaga tcgtcgcgaa gatcagcagg aacaggaccg gcaggagcgc
     1621 ggtgaagaag accgaggccg ggttccgcca gaacaccttc tgggcgaagc ggaactcgtg
     1681 gagtgcgagc gagacctgcc tcatgcgtcg gtcagctcca ggtagacgtc ttcgagactc
     1741 ggcctccgca cctcgagttg ctggatgtcg atcccctgcg cgatggccca ggcggtgatc
     1801 cggttcaggt cactgaccgc ctcatgggtg ctgacgctta tctccagcct gccgtcctcg
     1861 gccgttcggt cggaccgggt ctggccgaca ccctccaggt ccgacagatc ggttccgggc
     1921 ggcaggacga aagtgatcgt ggtcggcagg cgccggcgat cgccgaggtt ctcgggggtg
     1981 tccagggcca ctatctcgcc ctcgctcatg atcgccaccc ggtcggcgag gtactgggct
     2041 tcctccatgt agtgagtgg
//
LOCUS       DAOTIP010000390         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64593_length_2056_cov_3.310345, whole genome shotgun sequence.
ACCESSION   DAOTIP010000390 DAOTIP010000000
VERSION     DAOTIP010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64593_length_2056_cov_3.310345"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="P5138_07725"
     CDS             complement(<1..718)
                     /locus_tag="P5138_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60505.1"
                     /translation="MLLPSHPLALSTLLEPHNSQPSFMTSTIYGLGLETVGMSSQLPY
                     RLVAVFASLACVTLLYVLVRRHTDPWLALVMIVPVLGLGAAWEALLLALSMNFLIGIA
                     TGLGMLLALEFRSRRADLLACFLLCCSLTCGGVGLAFLVGACVDVLVRRDFRRVWVPA
                     VPVLLYAAWSFGFGDESSPASIGNALHLPEYLYRSAVAGVQALTGLGSQPLPDGPAHY
                     LTILLILALGAAALWRLLAAD"
     gene            871..1734
                     /locus_tag="P5138_07730"
     CDS             871..1734
                     /locus_tag="P5138_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60506.1"
                     /translation="MRPGPVESTAGDALTYGAVHLNVTDLNRSLVFWRDLIGLTELGT
                     EGEAVRLGVRDETDATDAALVVLHPGATRPVQRGYSALYHLAIHMPSEAAFARILGRL
                     FAARYPNAPTDHITHWATYLDDPDGINVEIAFETIDRVGKIDIVGGRPLIIDSEGRRH
                     DMTEPLGLEEVFSHRQDEDLTKPLPEGSRIGHVHLYVGDLEAAQAFYEEIGFTRHMDA
                     SGIGFADLSLGGSFPHRIAVNVWQGIGAPQAPEGTAGMRHFELFGESAGPAATEQADP
                     SGNRLVVLPRP"
     gene            1783..>2056
                     /locus_tag="P5138_07735"
     CDS             1783..>2056
                     /locus_tag="P5138_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HRV60507.1"
                     /translation="MRTLVDQRLSALRFASLRIVQICLAAGAAWLIADKVIGHYQPFF
                     APISVVLVLGLAIEQRGRRAYEIALGVALGIAIADLIVNVTGTGTWQ"
BASE COUNT          403 a          680 c          668 g          305 t
ORIGIN      
        1 gatcggcagc cagaagacgc cacaaagcgg ccgcgcccag agccagtatg agcaggatcg
       61 tcagatagtg ggccgggcca tcgggcagtg gctggctacc cagtccggtg agcgcctgga
      121 cgccggcgac ggcggaccga tacaggtatt ccgggaggtg cagggcgttc ccgatgctcg
      181 ccggggaact ttcatcacca aacccgaaac tccatgccgc gtacagcagg acaggcacgg
      241 caggcaccca gacgcgccgg aaatccctgc ggaccagaac gtcaacgcag gccccgacca
      301 ggaaggccag cccgacgccg ccgcaggtga gcgagcagca aagcaggaag caggccagca
      361 ggtcggcccg cctgctgcga aattccagcg ccagcagcat gccgagtccg gtcgcgattc
      421 cgatcaggaa gttcatggag agggccagca gaagggcctc ccaggccgcg ccgaggccaa
      481 gtaccggaac gatcatcacc agcgccagcc aggggtcggt gtggcggcga accagcacgt
      541 aaaggagagt cacgcaagcc agggaggcga acaccgcgac cagtcggtac ggcaactggg
      601 aggacatgcc gaccgtttcg agccccaggc cgtagatggt cgacgtcatg aacgacggct
      661 ggctgttgtg cggctccagc agggtagaaa gcgccagcgg atgcgacggg agcagcatct
      721 gccattcgtc gaaaaagaac gtcaggcctc tccccgccca gatgatcagg gaggcagaga
      781 agatcagaca tccgatcagg acgagaactg gtgcccggtt gcgcaagggc tgttattcaa
      841 ccggagtccg gcggctgcca tagttcaacc atgcgaccag gccccgtcga gagcaccgct
      901 ggcgacgccc tcacctacgg cgccgtccac ctcaacgtca ccgatctcaa tcgctccctg
      961 gtcttctggc gggacctgat cggcctgacc gaactgggga ccgaaggcga ggcggtacgg
     1021 ctcggggtga gggacgaaac cgatgccacc gatgcggcgc tggtagtcct ccaccccggc
     1081 gccacgcggc cggtgcagcg gggctactcg gccctctacc acctggctat ccacatgcct
     1141 tccgaggccg cattcgcccg gatcctcggt cgcctgttcg ccgcccgtta ccccaacgcc
     1201 ccgaccgatc acatcaccca ctgggcgacc tatctcgacg acccggacgg gatcaacgtc
     1261 gagatcgcct tcgagacgat cgaccgggtg gggaagatcg acatcgtcgg cggacgaccg
     1321 ctgatcatcg actccgaagg ccgcaggcat gacatgaccg agccgctcgg cctcgaggaa
     1381 gtgttctcgc accgacagga tgaggacctg acgaaacctc tgccggaggg cagccggatc
     1441 gggcatgttc acctctacgt gggtgacctc gaggccgccc aggctttcta cgaggagatc
     1501 gggttcacca gacacatgga tgcttccgga atcggctttg ccgacctctc gctcggcggg
     1561 agctttccac accggattgc ggtcaacgtc tggcagggca tcggtgcccc gcaagcgccc
     1621 gaaggcaccg ccggcatgag gcatttcgag ctcttcgggg agtcggcggg ccctgccgca
     1681 accgaacagg ccgatccttc cggaaaccgg ctggtggtcc tccccaggcc ctgagccgaa
     1741 agcgccctgc tttccggtca ccagcggtga gatagtggga ccgtgagaac gctggtggat
     1801 cagcgccttt cggccctgcg cttcgcttcc ctgcgaatcg tccagatctg tctggcggca
     1861 ggggcggcct ggctgatcgc tgacaaggtg atcgggcatt accagccctt cttcgcgccg
     1921 atctcggtgg tcctggttct cggtctggcg atcgagcaac gcggacgaag ggcctacgag
     1981 atcgctctcg gggtcgccct cggcatcgcc atcgccgacc tgatcgtgaa tgtgacgggc
     2041 accggcacct ggcagc
//
LOCUS       DAOTIP010000391         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64609_length_2056_cov_2.331334, whole genome shotgun sequence.
ACCESSION   DAOTIP010000391 DAOTIP010000000
VERSION     DAOTIP010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64609_length_2056_cov_2.331334"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="P5138_07740"
     CDS             complement(<1..1058)
                     /locus_tag="P5138_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV60508.1"
                     /translation="MSIRDLEALGSRAMNAPRVDRLREAVAGMPAWLVGGAVRDIALE
                     REVNDLDIAVAGPPEGPARKIAEALGGIAFELSEEFPAWRAKDRDDAWQVDVAELRAL
                     TIEQDLALRDFTVGAVAVGLEDGVALDPLGGLEDLGKGVIRACGPTSFTDDPLRLMRA
                     ARLVAQFGWEIDDETAGMARQAAPRAEDPAGERTLAELVLLIGARDPLAGLAAMDRIG
                     LFDWLFPEIGDLHDVVQGPNHHLDVYGHTMEVVEGILRIESDLERFTGDRASEVSEYL
                     ARPLADGATRAGALRLGALFHDCAKPQTRSEEGGFVSFRGHDRLGAEQIDARFRGLRA
                     SRKLIGYMSDLTRHHLILG"
     gene            complement(1055..>2056)
                     /gene="alr"
                     /locus_tag="P5138_07745"
     CDS             complement(1055..>2056)
                     /gene="alr"
                     /locus_tag="P5138_07745"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRV60509.1"
                     /translation="GALHCVGAVAESGADRIAFATATEAVLARSVAPDIPLLVLGALA
                     PGEIEMAISAGADISVWEPGFIATLADQAAASGLVVGVHVKYDTGMGRLGTTDPDLVM
                     ELARTADSHPNLRLAAIWTHFATADEEDPAFRDLQRDRLAKLGERARREFPGLKLHAA
                     NSAALIADPACHFDMVRPGVAIYGMDPFGRNPADHGLKPVLSFHSWVASVRDFEPGQS
                     AGYGRTWQAVSPTRVATVPVGYGDGVRRGLSNRGEVLIGGRRYPIAGTVSMDNITVDL
                     GPESGAKVGDEVTLIGTQGDETILAEQIAVPLETINYEVTCGISPRVPRVGTGSGSG"
BASE COUNT          315 a          750 c          667 g          324 t
ORIGIN      
        1 cccaggatca ggtggtggcg ggtcaggtcg gacatgtaac cgatcagttt tcggctggcc
       61 cggagcccgc ggaacctggc atcgatctgc tccgctccca gccggtcgtg gccgcggaag
      121 gaaacgaacc ctccctcttc gcttcgggtc tgcggcttgg cacagtcatg gaaaagggcc
      181 cccagcctca gcgccccggc gcgggtggcg ccatcggcga gcggccgggc caggtactcg
      241 gatacctcgc tggcccggtc accggtgaag cgttcgaggt cggattcgat ccggaggatc
      301 ccctccacca cctccatcgt gtggccatag acgtcgagat gatgattcgg cccttgaacc
      361 acgtcgtgaa gatcgccgat ctccggaaac agccagtcga agagtccgat ccggtccatc
      421 gcggccagtc cggccagcgg atcgcgggcc ccgatcagca gaaccagttc ggccagcgtt
      481 cgctctccgg ccgggtcctc ggcccgtggc gcggcctgcc gggccatccc tgcggtttca
      541 tcatcaattt cccatccgaa ctgggcaacg agccgggcgg cccgcatcag ccgcaggggg
      601 tcgtcggtga aggaggtcgg cccgcaggcc ctgatcactc ccttcccgag atcttcgagg
      661 cctccgagcg ggtcgagcgc caccccgtcc tccagaccga ccgccaccgc gccgaccgtg
      721 aagtcacgca gggccagatc ctgctcgatc gtcaatgccc tcagctcggc cacgtccacc
      781 tgccaggcgt cgtcgcggtc cttggcgcgc caggccggga actcctcgga gagctcgaat
      841 gcgatcccgc cgagcgcttc ggcgatcttc cgggccggtc cttccggggg tccggcaacg
      901 gctatgtcga ggtcgttcac ctcccgctcg agggcgatgt cgcggaccgc cccgccaacc
      961 agccaggccg gcattccggc caccgcctcg cggagccggt ccacccgggg cgcattcatc
     1021 gccctgctcc cgagcgcttc gagatcccgg atgctcaccc ggacccgctg cccgtgccga
     1081 cccggggaac ccggggcgag atcccgcagg tcacttcgta gttgatcgtc tccaggggca
     1141 ccgcgatctg ctccgcgagg atcgtctcgt cgccctgggt gccgatcagg gtcacttcat
     1201 caccgacctt tgcacccgac tccgggccga gatccacggt gatgttgtcc atcgagaccg
     1261 tgcccgcaat cgggtacctg cggccgccga tcaggacctc gccccggttg gaaaggcccc
     1321 gccgcacgcc gtctccgtaa ccgaccggga ccgttgccac acgggtcggg ctcacggcct
     1381 gccaggtacg gccatacccc gcgctctgtc ccggttcgaa gtccctgacc gaggcgaccc
     1441 aggaatggaa ggagagcacc ggcttcagcc cgtggtctgc cggattcctg ccgaaggggt
     1501 ccatcccgta gatcgccacg ccgggccgga ccatgtcgaa gtgacaggcg gggtcggcga
     1561 tcagggcggc actgttggcg gcatggagct tcaatcccgg gaactcccgc ctggccctct
     1621 ctcccagttt cgccagccgg tcgcgctgca ggtcccggaa ggcggggtct tcctcgtccg
     1681 cggtggcgaa gtgggtccag atcgccgcca gtctgagatt cggatgtgag tcagcggtcc
     1741 gggccagttc catcacgaga tccgggtccg tagtcccgag gcgacccatg ccggtgtcgt
     1801 acttcacgtg gacgccgacc accagcccgg aggccgcagc ctgatcggcc agggtcgcga
     1861 tgaagcccgg ctcccagaca gagatgtccg cgccggccga gatcgccatc tcgatctccc
     1921 cgggagcgag ggcgcccagc acgagcagcg ggatgtccgg agccacgctt ctggccagga
     1981 ccgcctcggt cgcagtggcg aaggcgatcc ggtcggcccc ggattccgcc accgcgccga
     2041 cgcagtgaag agcccc
//
LOCUS       DAOTIP010000392         2052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64760_length_2052_cov_3.562844, whole genome shotgun sequence.
ACCESSION   DAOTIP010000392 DAOTIP010000000
VERSION     DAOTIP010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64760_length_2052_cov_3.562844"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2052
                     /locus_tag="P5138_07750"
     CDS             <1..>2052
                     /locus_tag="P5138_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRV60510.1"
                     /translation="HHLVYEIVDNSVDEALAGHNESVEITLHPDNSCTVTDHGRGIPV
                     DIMEKEGRPAAEVVLTVLHAGGKFGDGGGYKVSGGLHGVGSSVVNALSTWLELRICRD
                     GFVWEQRYERGRPVTELVKGEKTKETGTQITFLPDDEIFESLDFDFQTLLERLRETAF
                     LTRGLRIQIKDERAEGQEVEFKYDGGIEDFVRYLNENKEPLGKKVVYFEGEDEEGQVE
                     VAMQWNASYNDSIHSFANNINTHEGGSHLSGFQAALTRTLNAYARSKAMLKEKDSNLA
                     GEDVREGLAAVISVKLHDPQFEGQTKTKLGNPGIKGMVQETVNRKLAEFLEENPTEAK
                     LFITKAIEASRARMAARKARDMTRRGSALENMNLPGKLADCSVRDPELAEIFVVEGDS
                     AGGSAKQARDRSTQAVLPLRGKIINVEKSRIDKVLQNNEIQAMITAIGTGIHDEFDIA
                     NARYHKIIMATDADVDGAHIRTLVLTFLYRQMQPLIDAGYVFIAKPPLYKVKRGNQET
                     YLDKESELEELLLRDRLEDFLITDSAGKETKLNSRRWQTYTRRSKEYEGWHAALQAEF
                     GNDVIGFISGSRLLDETATTVDEVRRILGEVDRDEDIFATEILSQDGEDTTVKAIHQR
                     SGLARNHTLPARLFEGDAFRRFAAVHAELLSTFGQPPFRVTLKDRAEEASDFLDLHRA
                     VL"
BASE COUNT          434 a          656 c          636 g          326 t
ORIGIN      
        1 caccacctcg tctacgagat cgtcgacaac tccgtcgacg aggccctggc cggtcacaac
       61 gagtcggtcg agatcaccct ccacccggat aacagctgca ccgtcaccga ccatggccgg
      121 ggaatcccgg ttgacatcat ggagaaggaa ggccggcccg cggcagaggt ggtactgacc
      181 gtccttcacg ccggaggcaa gttcggcgac ggcggcggtt acaaggtctc cggtggcctt
      241 cacggggtcg gctcctcggt cgtcaacgcg ctttcgacct ggctcgagct gcggatctgc
      301 cgcgacggct tcgtctggga acagcgctac gagcgcggcc ggccggtcac cgaactggtc
      361 aagggcgaga agacgaagga gaccggcacg cagatcacct tcctccccga cgacgagatc
      421 ttcgagtccc tcgacttcga cttccagacc ctgctcgagc ggttgcgtga aaccgccttc
      481 ctgacccgcg ggctgcggat ccagatcaag gacgagcggg ccgaaggcca ggaagtggaa
      541 ttcaagtacg acgggggaat cgaggacttc gtccgctacc tgaacgaaaa caaggagcct
      601 ctgggcaaga aggtcgtgta cttcgagggt gaggacgaag agggtcaggt agaggtggcg
      661 atgcagtgga acgcttccta caacgattcg atccacagtt ttgccaacaa catcaacact
      721 cacgagggtg gttcacatct ctccgggttc caggcggccc tgacccggac tctcaacgct
      781 tacgcccgca gcaaggcgat gctcaaggag aaggactcga acctggctgg cgaggatgtc
      841 cgcgagggcc tggcggcagt gatttcggtc aagctccacg atccccagtt cgaggggcaa
      901 acgaagacca aacttggcaa tcccggaatc aaggggatgg tccaggagac ggtcaaccgc
      961 aagctggccg agtttctcga ggagaacccg accgaggcga agctcttcat caccaaggcg
     1021 atcgaggcct caagggcccg aatggcagcc cgcaaggctc gtgacatgac ccgccggggc
     1081 tcggccctgg agaacatgaa ccttcccggc aagctcgccg attgctcggt tcgggacccc
     1141 gaactggccg agatcttcgt cgtcgagggc gattcggccg gcggttcggc caagcaggcc
     1201 cgcgaccgtt cgacccaggc ggtgcttccg ctgcgcggaa agatcatcaa cgtcgagaag
     1261 agccggatcg acaaggtcct gcagaacaac gagatccagg cgatgatcac cgcgatcgga
     1321 accggcatcc acgacgaatt cgacatcgcg aatgcccgct accacaagat catcatggcg
     1381 accgacgctg acgtggacgg agcccacatc cggacgctcg tgctcacctt cctctaccgg
     1441 cagatgcagc cgctgatcga cgcgggctat gtcttcatcg ccaagccccc gctctacaag
     1501 gtcaagcgcg gaaaccagga gacctacctc gacaaggaat cggaactgga ggagttgctc
     1561 cttcgtgacc gcctggagga ctttctgatc accgattcgg cgggcaagga aaccaagctg
     1621 aacagccgcc gctggcagac ctacacccgg cgctccaagg agtacgaagg ctggcacgca
     1681 gccctgcagg ccgagttcgg caatgacgtg atcgggttca tctccggttc ccggctgctc
     1741 gacgaaaccg cgaccacggt cgatgaagtg cggcggatcc tcggtgaggt cgaccgcgac
     1801 gaagacattt tcgccacgga gatcctctcc caggacggcg aagacacgac agtgaaggcg
     1861 attcaccagc gctccgggct ggcccgcaac cacacgcttc cggcccggct tttcgagggc
     1921 gacgcgttcc gccgtttcgc agccgttcac gcggagctgc tcagcacctt cggtcagccg
     1981 cccttccggg tgaccctcaa ggatcgggcc gaggaggcca gtgattttct cgacctgcac
     2041 cgggcggtcc tc
//
LOCUS       DAOTIP010000393         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_64921_length_2048_cov_4.670848, whole genome shotgun sequence.
ACCESSION   DAOTIP010000393 DAOTIP010000000
VERSION     DAOTIP010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64921_length_2048_cov_4.670848"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="P5138_07755"
     CDS             <1..406
                     /locus_tag="P5138_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRV60511.1"
                     /translation="DLEKELPLFEAGDFDVAVLSLTLQAVRRPDHVLTEMKRVAARLV
                     VSLPNFAHWRLRAFLALRGRMPVTDALPYQWYDTPNIHLCTIVDFENLTRELGLEIDR
                     RILIDATGRKTGGFARRAPNLLAERAVYSLTA"
     gene            510..600
                     /locus_tag="P5138_07760"
     tRNA            510..600
                     /locus_tag="P5138_07760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:543..545,aa:Leu,seq:gag)
     gene            complement(788..1276)
                     /locus_tag="P5138_07765"
     CDS             complement(788..1276)
                     /locus_tag="P5138_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60512.1"
                     /translation="MLPATDNRTGLIRRVQIASIMLTATLSLTAFGYWPFEGERARAK
                     PGTRIKVDDSQFGKVLYSGRGQAIYYFDREKSKRPRCYGSCAAAWPPVLTKGRPKALG
                     KVRQKLLGVTRRKGGGRQVTYAGHPLYYYAHEPRNVVFCHDVFQFNGLWLAARASGKP
                     VG"
     gene            complement(1362..>2048)
                     /locus_tag="P5138_07770"
     CDS             complement(1362..>2048)
                     /locus_tag="P5138_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRV60513.1"
                     /translation="VLADPFRIWSGKELLDTALARGSACDPGACFSYAHTNFLILARI
                     VRRILPQKNLVTLLNRRVLRPSGISMNFSRLAPIPPPALSAFATDRGIFEESTGWSPS
                     WGLGNGMLATTSIDNVSRIATGVLTGSTLSSWARKDLVKRYAPGLEPTPDRVYFAQGI
                     IMVNGWRRQNPFFNGYMGNVAWFPSRRIAISLVGTKGQSTTAPDGTNVTDAILGDMAG
                     YLTPANSPAL"
BASE COUNT          367 a          643 c          661 g          377 t
ORIGIN      
        1 tgacctcgag aaggaactgc ccctgttcga ggccggggac ttcgacgtgg cggtcctttc
       61 cctgaccctg caggcggtcc gccgtcccga ccacgtgctt accgagatga agcgggtcgc
      121 cgcccggctg gtcgtctccc tgccgaattt cgcccattgg cggctccggg ccttcctggc
      181 cctgcgcggc cggatgccgg tcaccgacgc gctcccctac cagtggtacg acactccgaa
      241 catccacctc tgcacgatcg tcgactttga gaacctgacc cgcgaactcg gcctggaaat
      301 cgaccgtcgc atccttatcg acgccaccgg ccgaaagacc ggaggcttcg cccgccgcgc
      361 cccgaacctc ctcgcagaaa gagccgtcta ctccctcacg gcctgaattc gcaacgagcc
      421 ctcaacttcc ttacagaact gcaagaagtc tgcgacggaa gtgcaaaatc tccggattcg
      481 aatgcgacag ctgagctaac ttcgagtcag cggagggtga agccgccagg cggcaatcgc
      541 ctgagaagcg agagttcggc ctaaccagcc ggatggggtt cgagtccccc ttcctccgcc
      601 agaaatttgc tggttcgggg gcgaggccgg gcagacaaga ctcaggtcgc ttcgggtggt
      661 tccccttcga cggcaacccc cgaatccagc aagtccggtg ctaaggtctt actacgtttc
      721 tcaggcggtt gggagtggtt ctcccgtcac ctcggaaagg ctcgcttcgg cgggcctttt
      781 cttttgatca gcccaccggt ttgccgctgg cacgggcggc cagccagagg ccgttgaact
      841 ggaagacgtc gtggcagaag acgacgttgc gaggttcatg cgcgtagtag tagagagggt
      901 gtccggcgta ggtgacctgc cggccgccgc ccttgcggcg ggttacgccg agcaacttct
      961 gccgcacctt gcccagggcc ttcgggcggc ccttggtcaa gaccggtggc caggcggcgg
     1021 cgcaggatcc gtaacagcgc ggacgctttg acttctcccg gtcgaagtaa tagatcgcct
     1081 ggccgcggcc ggagtagaga accttcccga actgggagtc gtccaccttg atcctcgttc
     1141 cgggcttggc ccgggcgcgt tcgccttcga aaggccaata gccgaaggct gtgagggaaa
     1201 gggtggctgt gagcatgatt gacgcaatct ggactcgtct gatcaggcct gttcggttgt
     1261 cggttgccgg aagcatggac caagcctcac cgaccccgat tacaaaacga tgaactggcc
     1321 cgcgaccccc ggcccgcccg gccatgggcc ggtcgccggg atcacagggc gggtgaattc
     1381 gcgggagtca ggtatccggc catgtcgccg aggatcgcgt cggttacgtt cgtcccgtcc
     1441 ggagccgtgg ttgactggcc cttggtaccg accagcgaga tcgcgatccg ccgggaggga
     1501 aaccaggcca cgttgcccat gtagccgttg aaaaacggat tctggcgacg ccatccgttg
     1561 accatgatga ttccctgcgc gaagtagacg cggtccggtg tgggctccag gccgggcgcg
     1621 taacgcttca ccagatcctt cctcgcccag cttgaaagag tgcttccggt gagtacgccg
     1681 gtggcgatcc ggctgacgtt gtcgatcgat gtggtggcca gcatcccgtt gccgagtccc
     1741 caggaaggag accagccggt tgattcctcg aagatccccc ggtcggtggc aaaggcgctc
     1801 agggcggggg gcgggatcgg agccagcctg gagaaattca tcgagatccc ggacggtctg
     1861 aggacccgcc tgttgagcag ggtcaccagg ttcttctggg ggagtatccg ccggacgatc
     1921 ctggcaagga tgaggaaatt ggtgtgggcg tagctgaagc aggcacccgg atcgcaggca
     1981 ctgcctctgg caagagcggt gtccagaagt tcctttccgc tccagatccg gaagggatcg
     2041 gccagcac
//
LOCUS       DAOTIP010000394         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_65169_length_2043_cov_2.744970, whole genome shotgun sequence.
ACCESSION   DAOTIP010000394 DAOTIP010000000
VERSION     DAOTIP010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65169_length_2043_cov_2.744970"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="P5138_07775"
     CDS             <1..572
                     /locus_tag="P5138_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60514.1"
                     /translation="MEGLDRAERRRFRSMIDRYLDENPARLTDLKRLRDPEFARMIGQ
                     VSTKLDGLGGGMSTLVVGLREQRQLLQEDVVAALARDLEASDAEEEKIDDYYHDLEFG
                     NLPAKGISGTLLSSLAKILGTTAERLKKAGEALGPSGSQASSLIYTRMADNAEEIDMG
                     QAAPAIEAPSGVGRRSDPPDRIDELFTGG"
     gene            635..1234
                     /locus_tag="P5138_07780"
     CDS             635..1234
                     /locus_tag="P5138_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRV60515.1"
                     /translation="MKVASLEPGEIDRIECEVGEIMAAVPPRVWDGESLPVPIDSIAR
                     EVYGLKVREVSHDQMREIVGDQATEGTMSGLLLTGIGEIWVNDWEARQPEWGSQRKRF
                     TIGHELGHFAMHQSGPPAIYCRASDSSESAEDAPTPHPVPELEANTFSAAFLMPASLV
                     RPEIGPEGVDGDQLKTLMLRFDSSAKAMRRRVETLRLIA"
     gene            complement(1236..>2043)
                     /locus_tag="P5138_07785"
     CDS             complement(1236..>2043)
                     /locus_tag="P5138_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60516.1"
                     /translation="PSNGFYAEGHDPVALGSNYRYTNLDVYTGDGRPDPETRPENAQP
                     GDSLSLLLEQVLKGQNDSAVAAAEAAGNPNVDYTVHKGSHNWEFWRPDLKAAISRGLF
                     GPATAQPSNWTYSTAASGGQAWDISFNFSPAPTAVTTFERKGNHLKVTGEGDLTIMDG
                     NGCWFTANLPFSRTLSDKPCRALKLKARGKGLRKGRKGTVTVTVKGLDSAAVTSPVDA
                     ARVTVAGKSALTSYNGKATLKVKPGKSGKLVVKVKKKGYRPAKRKLNVRR"
BASE COUNT          352 a          618 c          693 g          380 t
ORIGIN      
        1 tcatggaagg gctggatcgg gcggaacgcc gccggttccg ttcgatgatc gatcgctacc
       61 tggatgagaa tccggcgcgc cttaccgacc tgaaacgtct aagagaccct gagtttgccc
      121 ggatgatcgg gcaggtttcg acgaagctgg acgggctggg tggtggcatg tccactttgg
      181 tggttggcct gcgtgaacaa cgccagctgc ttcaggaaga cgtggtcgcg gccctggcca
      241 gggacctgga agcctctgac gccgaagagg agaagatcga cgactactac cacgacctcg
      301 aattcgggaa tctcccggcg aaaggcatct cgggcacatt gctgtcctcc cttgcgaaga
      361 ttctcggcac cacggcggag aggctgaaga aggcgggcga agcacttggc ccgtccgggt
      421 cgcaggcttc gagcctgatc tacacgcgca tggctgacaa cgccgaagag atcgatatgg
      481 ggcaggccgc cccggcgatc gaagccccgt ccggagtcgg acgaagatcg gatccgccgg
      541 atcggatcga cgagctcttc accgggggct gatttaccgg gccgtccggg tcgccggtaa
      601 aatcgtccgc gtgtccgggg taggagcggg agaaatgaag gttgccagtc tcgagccggg
      661 agagatcgac cgcattgaat gcgaagtcgg ggagatcatg gccgcggtgc cgccccgggt
      721 atgggacgga gagtcgttgc cggtcccgat cgactcgatc gcccgtgagg tctacggcct
      781 gaaggttcgc gaagtcagcc acgatcagat gcgcgagatc gtcggcgacc aggcgaccga
      841 aggcaccatg tcgggactgc tcctgaccgg gatcggagag atctgggtca acgactggga
      901 agccagacaa ccggagtggg gcagccagcg caagcgcttc acgatcggcc acgaactcgg
      961 tcatttcgcg atgcaccagt ccggcccccc ggcgatttac tgtcgagcct cggattcgag
     1021 cgagtcagcg gaagacgcac caacgcccca cccggtcccg gaactggagg cgaacacttt
     1081 ttctgccgcc ttcctgatgc ccgcctcgct ggtccggccc gagatcgggc cggagggcgt
     1141 ggatggtgat cagctcaaga ctttgatgct tcgcttcgac tcttcggcga aagcgatgcg
     1201 gcgccgggtc gaaactctca ggcttatcgc ctgagtcagc gccggacgtt cagcttgcgc
     1261 ttggccgggc ggtagccctt cttcttcacc ttgaccacca gcttgccgga tttccccggc
     1321 ttcaccttga gggtggcctt gccgttgtag gaggtgaggg ccgacttgcc tgccaccgtc
     1381 acccgggcgg catcaaccgg tgacgtgaca gcggcagagt ccaatccctt gacggtcacg
     1441 gtgacggtgc ccttacggcc cttgcgcagg cccttgccgc gagccttcag cttgagcgcc
     1501 cggcaaggct tgtcagacag ggtgcgtgaa aacggcaggt tggccgtgaa ccagcatccg
     1561 ttgccgtcca tgattgtcag atcaccttcg ccggtcacct tcagatggtt gcccttgcgc
     1621 tcgaatgtcg tgactgctgt gggggcgggg ctgaagttga aggagatgtc ccaggcctgg
     1681 cccccgctgg cggcagtgct gtaggtccag ttcgacggct gtgccgtagc cgggccgaac
     1741 aggcccctgg agatcgccgc cttcaggtca ggtcgccaga actcccagtt gtggcttccc
     1801 ttgtggaccg tgtaatcgac gttcgggttg ccggcggctt cggcagcggc caccgcggag
     1861 tcgttctggc ccttcagcac ctgttcgagc agcaggctga gcgaatcccc cggctgcgcg
     1921 ttctcgggcc tcgtctccgg gtcgggccgg ccgtccccgg tgtagacgtc gaggttggtg
     1981 tagcggtagt tggaaccgag cgcgaccggg tcgtggcctt ccgcgtagaa gccgtttgag
     2041 gga
//
LOCUS       DAOTIP010000395         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_65419_length_2037_cov_3.078204, whole genome shotgun sequence.
ACCESSION   DAOTIP010000395 DAOTIP010000000
VERSION     DAOTIP010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65419_length_2037_cov_3.078204"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="P5138_07790"
     CDS             complement(<1..273)
                     /locus_tag="P5138_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60517.1"
                     /translation="MAAVFDRPAPTSRQLLLGGGHIAALWALAFVQPLLDLLGKNPDF
                     FVARGNTTGDILILAIGFTLIPPLVLFGIEWVVSKVSARAYFGLHLA"
     gene            complement(324..1730)
                     /locus_tag="P5138_07795"
     CDS             complement(324..1730)
                     /locus_tag="P5138_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60518.1"
                     /translation="MTAAEPGSFRDRDSRVVITDDAIYRALSPEGAEDWEAFSGSALL
                     EKFTSAGQVIGTREVDLSVLGESSGLLPTGVTRVLEHDRVPFVSYPYEWTFGMLQDAA
                     KLQLELGIDAIDAGLTLKDATPYNVQFLGAHPTFIDVGSFEKIPEGEPWIAYRQFCML
                     YLYPLLFQAHKDLPFQPWLRGSIDGIQPIDAIKVFSLRDRLRRGVFLHTSLHARLDRR
                     ANKSGPGSAEENKTTRQVKPKQLQAQLQSMHRLVSKLKWKAGETSWSGYRQSNTYSDA
                     DDQRKQAFVAEVAAAAKPGLTWDMGCNDGAYSRIAAESSERVVAFDFDHATVEALYRS
                     LREEGNTKILPLVSNLADPSPALGWRGLERKTLADRGAPDLMLALALIHHVSISANIP
                     VADFLQWARDLETTLLIEFPKRTDPMVRALLANKHEGANPDYEEEIFERELESRFTVE
                     RREELPSGDRILYLARPR"
     gene            complement(1735..1947)
                     /locus_tag="P5138_07800"
     CDS             complement(1735..1947)
                     /locus_tag="P5138_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60519.1"
                     /translation="MHIFAYLDPGSVSAALAAVLAGIAGIGAAIRTFGSNLKSKLMFW
                     KKDEPAPESAATAATADTPEDKPAEQ"
BASE COUNT          325 a          618 c          711 g          383 t
ORIGIN      
        1 cgcgaggtgc agcccgaagt aggccctggc gctgaccttg ctgacgaccc attcgattcc
       61 gaacaggacc agcggcggga tcagggtgaa gccgatcgcg aggatcagga tgtccccggt
      121 ggtgttgccc cgggccacga agaagtcagg gttcttgcca agcaggtcga gcagcggctg
      181 gacgaaggcg agggcccaga gtgcggcgat gtggccgcca ccgagcagca gctgccggct
      241 ggttggtgcg ggacggtcga agaccgccgc cattcccgtt ttcggttgaa cttccgaact
      301 ctcgcccgga ccgttcactt cagctaccgg ggccttgcca ggtagaggat ccggtcgccc
      361 gaggggagtt cttcccgtcg ttcgaccgtg aaccggcttt caagctcccg ctcgaagatt
      421 tcctcctcgt agtcggggtt ggctccctcg tgcttgttgg ccagcagcgc ccggaccatg
      481 gggtcggtcc gtttcgggaa ctcgatcagc agggtggttt ccagatcccg ggcccactgc
      541 aggaaatcgg ctacggggat gttggcgctg atcgagacgt ggtggatcag ggccagggcc
      601 agcatcaggt cgggggcgcc gcggtcggcc agcgtcttgc gctcgagtcc gcgccagccc
      661 agggccggcg aagggtcggc gaggttggag accagcggga ggatcttcgt gttgccctct
      721 tccctgagtg accggtacag ggcctcgacc gtggcgtggt cgaagtcgaa ggcaaccacg
      781 cgctccgagc tctcggccgc gattcgcgag taggcgccgt cgttgcagcc catgtcccag
      841 gtcagtccgg gcttcgcggc tgccgcaacc tccgccacga atgcctgctt tcgctggtcg
      901 tcggcatccg agtaggtgtt cgactgccgg tatccgctcc aggaggtttc ccccgccttc
      961 cacttcagtt tcgagaccag ccggtgcatc gactggagtt gcgcctgaag ttgcttcggc
     1021 ttgacctggc gggtggtctt gttttcctcg gcgctgcccg gtccggactt gttggcgcgt
     1081 cggtcgagcc tcgcgtgaag ggaggtgtgg aggaagaccc cgcggcgcag gcggtcacgc
     1141 agcgagaaga ccttgatcgc gtcgatcggc tggatgccgt cgatcgagcc cctcagccat
     1201 ggctggaagg ggaggtcctt gtgggcctgg aagagcagcg ggtacaggta gagcatgcag
     1261 aactgccggt aagcgatcca gggctcgcct tcggggatct tctcgaagct gccgacgtcg
     1321 atgaaggtcg ggtgggcgcc gaggaactgg acgttgtagg gggtggcgtc tttgagggtg
     1381 aggccggcgt cgatcgcgtc gatgcccagt tccagctgaa gcttcgccgc gtcctgcagc
     1441 atgccgaagg tccattcgta ggggtaggag acgaacggca cccggtcgtg ctccagcacc
     1501 ctggtcactc cggtcggcaa gagaccggag ctctcgccga gcaccgaaag gtcgacctcc
     1561 ctcgtgccga tcacctgacc ggcggaagtg aacttctcca gcagggcact gccggagaac
     1621 gcttcccagt cctcggctcc ctccgggctc agtgcccggt aaatcgcgtc gtcggtgatg
     1681 acgacgcggc tgtcacggtc tctgaacgag cccggttcgg cggccgtcac agccttactg
     1741 ctcggcgggc ttgtcttcag gggtgtcggc agtggccgcg gtggccgctg attccggcgc
     1801 aggctcgtcc ttcttccaga acatgagctt gctcttcagg ttgctcccga aagtgcggat
     1861 ggctgcaccg atgccggcaa tgccggccag cacggcggca agggcggcgc tgacgctgcc
     1921 ggggtcaagg taagcaaaga tgtgcatgag tgatcctctc ttgagcggga tcggcggggc
     1981 cttcactcaa gggcagaccc cgaccgaccg agaaatgtta ctcaatagtt acgaggg
//
LOCUS       DAOTIP010000396         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_65422_length_2037_cov_3.017659, whole genome shotgun sequence.
ACCESSION   DAOTIP010000396 DAOTIP010000000
VERSION     DAOTIP010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65422_length_2037_cov_3.017659"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="P5138_07805"
     CDS             <1..199
                     /locus_tag="P5138_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60520.1"
                     /translation="PDLVTPASKKTLAIGDYRLVVRAGNRLMKRLRRTARPRSVRLKV
                     TFRPVGGRKSARTRKARFVLR"
     gene            complement(215..1621)
                     /locus_tag="P5138_07810"
     CDS             complement(215..1621)
                     /locus_tag="P5138_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60521.1"
                     /translation="MASGLTRRELGRATLAGAALAGLGGAGLLAGCGSGEGEDSAEAS
                     KQDVIDRLPSMGIPKPVDYIRTDWSHDPLALCSYSYLAPSRYGSTVRRMLAEPFGRVV
                     IAGEATARIPATVHGAIGAGREVAALVGRELGEEGRVAVIGAGAAGLACAGALEQGGL
                     RPVLIEASGRVGGRVRSGELAGSPVELGASWIHGVEGNPLTDLADAAGVGLHPFGYEP
                     GFPVPGQRALADAGERRLFRAIDSFDPEVPGARLRTVDWLLPYRWGPGLQWAVQNEIA
                     QEYGADADRLAALATWEGGWLGGGDALLDGPYASLLGGLAGNTEIRLDSPVSAIHQGS
                     EGVTVSAAGGEIEADAAVVTVPIGVLKAGTIEFTPQLPARTRTAIETLGAGSCGVNSM
                     VPAFKEPFWEREVEGFAWIDPRRPGRWGSWVNSVPVNGKPFLLTLSGGGDAHRLARRS
                     DLEVVGGAMAALQSMFAG"
     gene            1736..>2037
                     /locus_tag="P5138_07815"
     CDS             1736..>2037
                     /locus_tag="P5138_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60522.1"
                     /translation="MSESAARDDQGRADATEATRAANDSLAASLNFEDTRDFDEAARG
                     LVAPLPDDGRVTSEDGGLVWDLSRFEFIHKHDEAPPTVNPSLWRQTRLVVQGGLYE"
BASE COUNT          357 a          731 c          649 g          300 t
ORIGIN      
        1 acccgatctc gtaaccccgg cgtcgaagaa gaccctggcg atcggtgact accggctggt
       61 ggtcagggcg ggcaaccggc tgatgaagcg actcaggcgg accgccaggc cccggtcggt
      121 gaggctgaag gtcaccttcc gcccggtggg tggccggaag tcggccagaa cgcgcaaggc
      181 gcgtttcgtt ctgcgctgac ccgaggcagg gacctcagcc cgcgaacatg ctctgaagcg
      241 ccgccatcgc gccgccgacc acctccaggt cgctgcggcg ggcgaggcga tgggcgtcgc
      301 ctccgccgga gagggtgagc aggaacggtt tcccgtttac cggtaccgag ttgacccagc
      361 ttccccatcg tcccggccgc cggggatcga tccaggcgaa tccttccact tccctttccc
      421 agaagggctc cttgaaagcc gggaccatcg agttcacgcc gcaactgccc gccccaagag
      481 tctcgattgc ggtccgggtc cgggcgggca gttgcggcgt gaactcgatg gtcccggctt
      541 tcagcacccc gatcggcacg gtcactaccg ccgcgtcggc ttcgatctct cctccggccg
      601 cggaaacggt gaccccttcc gacccctggt ggatcgcgct caccggactg tcgagccgga
      661 tttccgtgtt ccccgcaagt ccgccgagca ggctggcgta gggtccgtcc agaagggcgt
      721 cacctccgcc gagccaccct ccttcccagg tggcgagtgc ggcgagccgg tccgcgtcgg
      781 ccccgtactc ctgggcgatc tcgttctgca ccgcccactg gagcccgggc ccccacctgt
      841 aggggagcaa ccagtcgacc gtgcgaagcc tcgccccggg aacttccggg tcgaaggagt
      901 cgatcgcccg aaagagacgc ctttccccgg catcggccag cgcccgctga cccggaacgg
      961 gaaagcctgg ctcgtatccg aacgggtgga ggccgacacc ggccgcatcg gcaagatccg
     1021 tgagcggatt gccctcgacc ccgtgaatcc aggaggcgcc cagttcaacc ggcgagccgg
     1081 ccaattcgcc gctccggacc cggccgccaa cccggcccga tgcttcgatc aggaccggcc
     1141 gcaatccgcc ctgctcgagc gcgcccgcgc aggcgagccc ggcggcacct gccccgatca
     1201 ccgcgaccct gccttcctca cccagctccc gtcccaccaa cgccgcgacc tcgcgacctg
     1261 ccccgatcgc tccatgaacg gtggcgggga tccgcgcggt cgcttcaccg gcgatcacga
     1321 cccggccgaa gggttcggcg agcattctgc ggaccgtcga tccgtaccgg ctgggcgcaa
     1381 ggtatgagta ggaacagaga gcgagcggat catgggacca gtcggtccgg atgtagtcga
     1441 cgggtttcgg gattcccatc gacggaagac ggtcgatcac atcctgcttc gaagcttccg
     1501 cggagtcttc tccctcgccc gaaccgcagc cggcgaggag acccgcgccg ccgagcccgg
     1561 ccagcgcggc cccggccagg gtcgccctgc caagttcacg tcgggtcagc ccggaagcca
     1621 cgcactaatt ctcccctgaa acccgaaaag ccgccagaaa cgatttcaat tcgggcgcat
     1681 atgggcaggg agataacctc ctttcgcggc caaaattacg aggaaggaac tacacatgag
     1741 cgaaagcgcg gccagggacg accagggacg ggcagatgcc accgaggcaa ccagggcggc
     1801 gaatgattcg ctcgccgcga gcctcaactt cgaagacacg cgggacttcg acgaggcagc
     1861 ccgcggcctc gtggcaccgc tcccggacga cgggcgggta actagcgaag acggcggtct
     1921 ggtctgggat ctctcgcgct tcgagttcat ccataagcac gacgaagcac cacctaccgt
     1981 caacccgagt ctttggcggc agacccgtct ggtcgtccag ggcgggctct acgagat
//
LOCUS       DAOTIP010000397         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_65545_length_2034_cov_3.289035, whole genome shotgun sequence.
ACCESSION   DAOTIP010000397 DAOTIP010000000
VERSION     DAOTIP010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65545_length_2034_cov_3.289035"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="P5138_07820"
     CDS             complement(<1..659)
                     /locus_tag="P5138_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HRV60523.1"
                     /translation="MSDTYTQIVNAPVLSNLASSLGLPQPVELDRFGPGQPVIKGPVL
                     TSGAPEGRLRPAISAILDSIGAERAGLEGPAKALVFDATGIADSTQLVELQRFFYPAV
                     PRLSRSGRAIVLGSPPRSTGGVRAHTAQRALEGFVRSLGKEIGGRGSTAQLVQVEPGA
                     EGELESTLRYLLSPRSAFVSGQVIRVGVPVTSRPDINWDLPLAGLTALVTGASRGIGE
                     S"
     gene            complement(656..1948)
                     /locus_tag="P5138_07825"
     CDS             complement(656..1948)
                     /locus_tag="P5138_07825"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRV60524.1"
                     /translation="MKKKTRKVAVVGANRIPFARSNGTYTHASNQDMLTAALDGLIDR
                     FALDGERLGEVAGGAVLKHSRDRDLTRESVLGTRLAAETPAYDVQQACGTGLETTILV
                     ANKIALGQIDAGIACGADTTSDAPISLNEELRETLLEANRQTTTLGRIRALTNVRPGH
                     LVPSIPRNEEPRTGLSMGEHCAIMAKDWDIVRTEQDQLAVDSHHRLAAAYERGFFEDL
                     ITPYLGLRQDQNLRPDSSLEKLSKLKPVFGGPEGTMTAANSTPLSDGASAVLLASEDW
                     AKERGLPILAYVEEAQTAAVDHVHKREGLLMAPAYAMSEMLGRTGLGLQAFDYYEIHE
                     AFAAQVLCTLKAWESDDFCREKLGRSEAMGPIDRSKLNVNGGSLAAAHPFAATGGRIV
                     GALAKQLHEDGKGRGVISICAAGGQGVVAILEAPGGNK"
BASE COUNT          302 a          638 c          741 g          353 t
ORIGIN      
        1 atcgattcac cgatcccgcg cgaggcacct gtgaccaggg cggtgagccc ggcaagcgga
       61 agatcccagt tgatgtcggg ccggctggtg accggaacgc ctacacggat cacctgtccc
      121 gagacgaagg cagagcgggg ggagaggaga taacgcaagg tcgactcgag ctctccttcg
      181 gctcccggtt cgacctgcac tagttgcgcg gtcgagccgc ggccgccgat ctccttgccg
      241 agcgagcgga cgaacccttc gagtgcccgc tgggcggtgt gggcgcggac gccgccggtc
      301 gaccggggtg gcgaacccag gacgatcgcc cggccgctcc gtgacagtcg cgggaccgcc
      361 ggatagaaga agcgctgaag ttcgaccagc tgggtcgagt cggcgatgcc ggtcgcgtcg
      421 aagaccaggg ccttggccgg gccttcgagg ccggcccgtt cggcaccgat cgaatcgagg
      481 atcgcgctga tcgcgggacg caggcggccc tcaggggcgc cactggtcag taccggaccc
      541 ttgatcaccg gctggcccgg gccgaatcgg tccagttcga ccggctgcgg caggcccagc
      601 gaactggcca ggttcgagag gaccggcgcg ttgacgatct gtgtgtaggt atcgctcact
      661 tgttgcctcc aggcgcttcg aggattgcca cgacgccctg gccgccggcg gcgcagatgc
      721 tgatcacacc ccggcccttg ccgtcttcat ggagttgctt ggcgagggcg ccgacgatcc
      781 ggccgccggt cgccgcgaag gggtgggcgg ccgccagcga gccgccgttc acgttgagct
      841 tcgagcggtc gatcgggccc atcgcttcgc ttcggcccag tttctcgcgg cagaaatcgt
      901 cgctctccca ggccttgagg gtgcagagca cctgtgcggc gaaggcctcg tgaatctcgt
      961 agtagtcgaa ggcctggagg cccagtccgg tccgtccgag catttccgac atcgcgtagg
     1021 cgggtgccat caggaggcct tcgcgcttgt ggacgtggtc gacggcggca gtctgggctt
     1081 cctcgacata ggcgaggatc ggcaggccgc gttccttcgc ccagtcttcc gaggccagca
     1141 gaacggccga cgcgccatcc gagaggggag tggagttggc ggcggtcatc gtgccctcgg
     1201 ggccgccgaa taccggtttc agcttcgaca gcttctccag ggaggaatcg ggccgaaggt
     1261 tctggtcctg cctcaggccg aggtacggag tgatcaggtc ctcgaagaaa ccccgctcgt
     1321 aggcggcggc gaggcggtgg tggctgtcga cggcgagttg atcctgctcg gtccggacga
     1381 tgtcccagtc cttcgccatg atcgcgcagt gctcgcccat ggaaagcccg gttcggggct
     1441 cctcgttgcg gggaatgctg ggaaccagat ggccgggccg gacgttggtc agcgccctga
     1501 tccgtccgag agtcgtcgtc tgccggttgg cttcgaggag ggtctcgcga agctcctcgt
     1561 tcagcgagat cggggcatcg gacgtcgtat cggcgccgca ggctatcccc gcgtcgatct
     1621 gaccgagggc gatcttgttg gcgaccagga tggtggtctc gaggccggtg ccgcaggcct
     1681 gctgaacgtc ataggccggg gtctctgccg caagacgggt gccgagcacg ctttcgcggg
     1741 tcaggtcccg gtcccgggag tgcttcagca ccgcgccgcc ggccacctcg ccgaggcgct
     1801 cgccgtcgag ggcgaaacgg tcgatcaggc cgtcgagggc ggcggtcagc atgtcctggt
     1861 tcgaggcatg ggtgtaggtc ccgttcgacc tcgcgaacgg gatccggttg gcgccgacta
     1921 cggcaacctt gcgggtcttt ttcttcatgc ggaggggtct ccttcggtta ctgcgggttg
     1981 ggagggggcg gtgcgggcca gcaggcccgg ctccaccgtg gaaatgagaa gttc
//
LOCUS       DAOTIP010000398         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_65690_length_2031_cov_2.863866, whole genome shotgun sequence.
ACCESSION   DAOTIP010000398 DAOTIP010000000
VERSION     DAOTIP010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65690_length_2031_cov_2.863866"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..916)
                     /locus_tag="P5138_07830"
     CDS             complement(230..916)
                     /locus_tag="P5138_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV60525.1"
                     /translation="MSGEGTATVSRRVHDSLRRQILEGTLSPGDRIPSERVLAEEFGV
                     NRHAVREALKGLQQAGLIRITHGGATRVLDWRDSGGLEVLLDLGGDPVDPPAEIVRSV
                     LEMRASIGVDAARLCAGRASEAERDEVERLGERAAALVGAGDDDLLDQSFAEFWMAVV
                     SGSGNIAYRLSLNSLLAAMLSFGEVANRIRPDDPKIITRLGSAIGAGDPDTAAEVAGH
                     MLHADVKRAI"
     gene            993..1493
                     /locus_tag="P5138_07835"
     CDS             993..1493
                     /locus_tag="P5138_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRV60526.1"
                     /translation="MRRLRPVLTGIVLALGFQAIVRKLLLTKFRRSVEALNEGDYGPL
                     LDGFADDAVLHFNDAPHRWAGEHRGREQIELFLRDFVAAGLQGEILEFWTSGPPWALT
                     AVAQFDDISIGPDGEALYTNQTMILVRTRWGKVVEQRDFYVDTGRMIDFDRKLTELGI
                     EPVARN"
     gene            1507..>2031
                     /locus_tag="P5138_07840"
     CDS             1507..>2031
                     /locus_tag="P5138_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV60527.1"
                     /translation="MTPVFTPFHHGGSGPPLVLIHGITDTWSTWELVIPPLERKFEVL
                     AVTLPGHAGGPALKGDFTADTLPAGIEAAMDGVGFGSAHVAGNSLGGWVALRLAERGR
                     ALSVSAFAPVGGWSADFQERHAFDYFVTQQELVKRAAPFAREIAATPEGRRQATRFIV
                     EDDSLITAEMVVRQL"
BASE COUNT          351 a          675 c          663 g          342 t
ORIGIN      
        1 gtgtccttcg cctcgaaccc gatcggcgca tccgggtcga tcgcggcctg ctcttcggca
       61 tgttcgtggg ccgcgtgccg gagggtcagg tattccagac ccatagccag aaagaagaac
      121 gggatcgcat agaggatcag gtcgctcatg actcaactgg ttataccagt tgcatgttgt
      181 gttggcaagt ggccggccgg gacctggccg cggccggaac gcgggatggt cagatggcgc
      241 gcttcacgtc ggcgtggagc atgtgaccgg cgacctcggc cgccgtgtcc ggatccccgg
      301 cgccgatcgc gctgccgagg cgggtgatga tcttcggatc atcgggtcgg atcctgttcg
      361 cgacctcacc gaaggagagc atcgccgcca gtagcgaatt gagcgaaagg cgataggcga
      421 tgttgccgga accggaaacg accgccatcc agaactcggc gaaggactgg tcgagcaggt
      481 cgtcgtcgcc tgcgccaacg agggcggccg cccgctctcc cagccgttcg acttcatcgc
      541 gttcggcctc gctggctcgc ccggcgcaga gccgggcggc atccaccccg atcgaggccc
      601 tcatctcgag aaccgagcgg acgatctcgg ccggcggatc gaccgggtcg ccgccaaggt
      661 caagcagcac ctccagcccg cccgaatcgc gccagtcaag aacccgggtg gcgccgccgt
      721 gggtgatcct gatcagaccg gcctgttgaa gccccttgag cgcctcgcgc acagcatgcc
      781 ggttgacccc gaattcctcg gccagtaccc gctcggaggg gatccggtcc cccggagaga
      841 gggttccctc aaggatctga cgccgcagcg aatcgtggac ccgccgggaa accgttgctg
      901 tgccttcacc gctcatctga aggaggttct accaactgga atgcgggtaa cggtcggata
      961 ggttggattc aacccctcga gaaaggctcc tcatgcgccg cctccgaccg gtattgaccg
     1021 gcatcgttct cgcccttggc ttccaggcca tcgtccgaaa gctgctcctg acaaagttcc
     1081 gccgcagcgt cgaagccctc aacgaaggcg actacggccc gctactggac ggcttcgccg
     1141 atgacgccgt cctccacttc aatgacgcgc cccaccgctg ggccggcgag caccgcggac
     1201 gcgaacagat cgagctgttc ctccgggact tcgtggcggc cgggctccag ggggagatcc
     1261 tcgagttctg gacctccggc ccgccctggg cactgaccgc ggtagcgcag ttcgacgaca
     1321 tctcgatcgg tccggacggc gaagcgctct atacgaacca gacgatgatc ctggtccgca
     1381 caagatgggg gaaagtggtc gagcagcgcg acttctacgt ggacaccggg cgcatgatcg
     1441 acttcgaccg caagctcacc gaactcggga tcgagccggt cgcccgcaat tgaaatagtc
     1501 ccggtcatga caccggtgtt cacgcccttc catcacggcg gttcgggccc gccgcttgtg
     1561 ctcatccacg ggatcaccga cacctggagc acctgggagt tggtcatacc cccgctggag
     1621 cggaaattcg aggtgctggc cgtgaccctg cccggacatg ccggcggacc ggcactcaag
     1681 ggcgacttca ccgccgacac gttgccggcg ggaatcgagg cagccatgga tggagtcgga
     1741 ttcgggtccg cgcatgtggc cggcaattcg ctcggtggct gggtggcgct ccggctcgcg
     1801 gaacggggtc gggccctttc ggtgtccgct ttcgccccgg tcggcggctg gtcggccgat
     1861 ttccaggagc ggcatgcctt cgactatttc gtcacgcagc aggaactggt caagagagcg
     1921 gcgcccttcg ccagggagat cgccgcgacc ccggaaggcc gtcgccaggc gacccggttc
     1981 atcgtcgagg acgacagcct gatcaccgcc gaaatggtgg ttcgccagct g
//
LOCUS       DAOTIP010000399         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66170_length_2020_cov_3.664631, whole genome shotgun sequence.
ACCESSION   DAOTIP010000399 DAOTIP010000000
VERSION     DAOTIP010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66170_length_2020_cov_3.664631"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="P5138_07845"
     CDS             complement(<1..424)
                     /locus_tag="P5138_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60528.1"
                     /translation="MLFLARRGPGRGLLPALLACLVVAPFLVGCGEDRSNLIPKDTSE
                     SLIARFDRIQSLAAAGECFQAAKVARTAQQEIEAMGTDVDAELKRSLLDGVTDLTLLV
                     NDPDKCTEADTTTTEVPVETEETPTDTEGTTGETGTTDT"
     gene            complement(479..1216)
                     /locus_tag="P5138_07850"
     CDS             complement(479..1216)
                     /locus_tag="P5138_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRV60529.1"
                     /translation="MNVAVISDIHANRHALDAVLEDIDATGVDEIWFLGDAVGYGAFP
                     GECVTTLKDRCSVFLLGNHDLAALREIDIGTFSPSAAMSARWTRDNMEPEAFEILRSL
                     DGTSAVREDIGLFHASPRDPIWEYVVDSDLAEDNLDFQEERIALIGHSHIALYFTRVD
                     EMSRVSSVLAPQGTELTMTGGKWLLNPGSVGQPRDGDPRAAWAELDTEEFRARFHRVD
                     YPIDEAAEAIREAGLPTHLGDRLFKGQ"
     gene            1251..>2020
                     /locus_tag="P5138_07855"
     CDS             1251..>2020
                     /locus_tag="P5138_07855"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60530.1"
                     /translation="MNSGPATGRGVNGYVLAGTAVGALLVAVIAALLLSPDQGLPVPD
                     PAAAARVIADRFTPAELSQAGDYRSWGRLIGVATLITQFAALGFLAFWRGGPMRRMLE
                     RLGRRPLVGAQAVGAGISLVLALVDLPFNVAAWKLGRDYGLISQALGPRLFDWLLSAL
                     ITMVPAALGALIAMALWRRLRGRFWIAASALIAVWAVVTTWLWPVVVAPIFNDFEPLP
                     AGPARSEVLRLADRAGVEVGEVYVVDASRRSSTVNAYV"
BASE COUNT          298 a          641 c          694 g          387 t
ORIGIN      
        1 tggtgtcggt ggttccggtc tccccggttg tgccttcggt gtctgtcggt gtctcttcgg
       61 tctcgaccgg tacctcggtg gtcgtcgtgt ccgcttcggt gcatttgtcc gggtcgttca
      121 caaggagcgt caaatcagtg acaccatcca acagggaacg tttcagctcc gcgtctacat
      181 cggttcccat cgcttcgatc tcctgctgcg cggtccgggc cactttggcc gcctggaaac
      241 actctccggc tgccgcgagc gactggatcc ggtcgaagcg ggcgatcagc gattccgacg
      301 tgtccttggg gatcaggtta ctgcgatcct cgccgcagcc aaccagaaac ggggctacga
      361 ccaggcaggc gagcagggca ggcagaagac ccctgccggg gcctcttcgg gcaagaaaca
      421 gcattccggg cgagtctaga tggttgaaac gcttgccttt gtgatgcatt cccggccttt
      481 actgaccctt gaagaggcgg tcgcctaggt gggtgggaag gccggcttca cggatcgctt
      541 cggccgcctc gtcgatcggg tagtcgaccc ggtgaaagcg cgccctgaac tcctccgtgt
      601 cgagttccgc ccaggcagcc cgcgggtcgc cgtcgcgggg ctgtccgaca ctgccggggt
      661 tgagcagcca cttgccaccc gtcatcgtca attcggttcc ctgcggtgcc aggaccgacg
      721 agacccgcga catctcgtcg accctcgtga aatagagcgc gatgtgcgaa tggccgatca
      781 gggcgattcg ctcctcctgg aagtcgagat tgtcctcggc caggtcgctg tcgaccacgt
      841 actcccagat cgggtcgcgc ggcgaggcgt gaaagaggcc gatgtcttcc cggaccgccg
      901 aggtgccgtc caggctgcgc aggatctcga aagcctcggg ctccatgttg tcgcgcgtcc
      961 acctggccga catcgcggcc gaggggctga aggtgccgat gtcgatttcc ctcagcgccg
     1021 ccaggtcgtg gttgccgagc aggaagaccg agcagcgatc cttcagggtg gttacgcatt
     1081 ccccggggaa cgccccgtat ccgaccgcat caccgagaaa ccagatctcg tcgaccccgg
     1141 tggcatcgat gtcctcaagg accgcgtcga gcgcgtggcg gttggcgtgg atgtcggaaa
     1201 tgacggcgac gttcatggtc gggcttgccc gccgaatatc gtattgagcg atgaattcgg
     1261 gtccggccac cggccgcgga gtgaacggct acgttctggc aggaaccgct gtcggagctc
     1321 ttctggtggc cgtgatcgcg gccctccttc tgagcccgga ccagggcctg ccggttcccg
     1381 acccggcggc cgcggcgagg gtgatagcgg accgtttcac cccggcggag ctgagccagg
     1441 ccggggacta ccggtcctgg ggacggttga tcggggtagc gacgctgatc acccagtttg
     1501 ccgccctcgg cttcctggcc ttctggcggg gcgggccgat gaggcggatg ctggagcgac
     1561 tcggcaggag accactggtc ggtgcccagg ccgtcggggc gggcatatcc ctcgtgctgg
     1621 cgctggtcga cctgccgttc aacgtcgccg cctggaaact gggccgggac tacggactga
     1681 tcagccaggc actcggtccc aggctgttcg actggctgct ctcggccctg atcacgatgg
     1741 ttccggcggc gctcggggca ctgatcgcga tggccctctg gcgccggctc aggggccggt
     1801 tctggatcgc tgcttcggcg ctaatcgcgg tctgggccgt cgtcacgacc tggctttggc
     1861 cggttgtggt cgccccgatc ttcaacgact tcgagcccct gccggccggg ccggcgcgga
     1921 gcgaggtgct gcgactggcg gaccgggcgg gtgtcgaagt gggggaggtc tatgtggtgg
     1981 acgccagccg cagatcctcc acggtaaatg cctacgtgaa
//
LOCUS       DAOTIP010000400         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66173_length_2020_cov_3.333333, whole genome shotgun sequence.
ACCESSION   DAOTIP010000400 DAOTIP010000000
VERSION     DAOTIP010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66173_length_2020_cov_3.333333"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..109
                     /locus_tag="P5138_07860"
     tRNA            37..109
                     /locus_tag="P5138_07860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70..72,aa:Val,seq:gac)
     gene            complement(173..1573)
                     /locus_tag="P5138_07865"
     CDS             complement(173..1573)
                     /locus_tag="P5138_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60531.1"
                     /translation="MPGRKSALWLAAAISVLTFTTAGLASAATRERPCNGSVELCSRT
                     LDQVVLPGTHNSMSNEEYGWAIPNQHYSIPTQLGYGVRAFLIDTHYGKPSPGTGVSGV
                     ANWKGSDGDPHTNNAGVYLCHVFCTLGASDLTTELGKIANFLASHPREVLVFQVENYV
                     DPEDIASSVSDSGLIDYVYTGSTDQYPTLGEMIAENQRVVMFSEGNTGSVPWFHDSYA
                     GAMQETPYDFRRNEANVPLSTQGGIDLLTNPATLDSTCRPHRGGTTGSIFLMNHWVNG
                     NIDDGPDGTLDVTPDPAVAQILNQPDVLIARAEACKARRGKLPNIVAVDDFGEGDLTD
                     AVRQLNGVKAKPFFEVTRPRKATVKAGRKATYKIAIRNWGDGESALTRVCASVPKKLA
                     VKPKCVSAYVSQGNPGKGTAILKISTRRIIQGRFKGSKSGQVRFTISGGGDSISTTAA
                     LKVKPLKKKKPKRKRH"
     gene            1637..>2020
                     /locus_tag="P5138_07870"
     CDS             1637..>2020
                     /locus_tag="P5138_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60532.1"
                     /translation="MESRGVIKLAQSILGIAVRSLFALPDRLMALIFGTPPAEAAALR
                     PDAWAMGRVIGLIEDRRTDWDPEEAREETDFLAGAVSLAVPEVISQDWVLEDGQRGLP
                     ARLFIPESNPLAGPMLLHFHGGGWVQ"
BASE COUNT          309 a          621 c          681 g          409 t
ORIGIN      
        1 cgacggatcc ggcgctgcgc taggctctcc gtccccgggc gcatagctca gttggtagag
       61 cgccacctcg acacggtgga ggtcactggt tcgagcccag ttgtgcccac tcaggaacag
      121 gaaacccggc tcgtcggccg ggtttcgtcg ttgaggagag cctgatcagc gctcagtgac
      181 gcttgcgctt cggctttttc ttcttcagcg gcttgacctt gagcgcagcg gtggtggaga
      241 tgctgtcgcc gccgcccgag atcgtgaacc tgacctggcc cgacttgctg cccttgaacc
      301 ttccctggat gatgcgacgg gtggagatct tcaggatcgc cgttcccttg cccgggttgc
      361 cctggctcac ataggccgac acgcacttgg gcttgacggc cagtttcttc ggcaccgagg
      421 cgcagaccct ggtcagcgcg gactcgccgt cgccccagtt ccggatcgcg atcttgtagg
      481 tcgccttcct gccggccttg accgtggcct tgcggggccg ggtcacttcg aagaacggct
      541 tcgccttcac gccattcagt tgccgtacgg cgtcggtcag gtctccttcg ccgaagtcgt
      601 cgaccgcgac gatgttcggg agcttgccac gtcttgcctt gcaggcctcg gcccgggcaa
      661 tcagcacgtc gggctggttg aggatctggg cgaccgccgg gtcgggggtg acgtccagcg
      721 tgccgtcagg tccgtcgtcg atgtttccgt tgacccagtg gttcatcagg aagatcgaac
      781 ccgtcgtgcc gccgcggtgc ggccggcagg tcgagtccag ggttgccgga ttggtcagga
      841 ggtcgatgcc cccctgggta ctgaggggca cattcgcttc gttcctgcgg aagtcgtagg
      901 gggtctcctg catggcgccg gcgtagctgt cgtggaacca ggggacgctt ccggtgttgc
      961 cctcggagaa catgaccacc cgctggtttt cggcgatcat ttcgccgagt gtcgggtact
     1021 ggtcggtcga gccggtgtag acgtaatcga tcaggccgct atcgcttacc gagctggcga
     1081 tgtcttcggg atcgacatag ttctcgacct ggaagaccag cacctcccgg gggtgacttg
     1141 cgaggaagtt ggcgattttg cccaactcgg tggtcaggtc cgacgcaccg agggtgcaga
     1201 acacatggca gaggtagaca ccggcgttgt tcgtgtgcgg atcgccgtcc gatcccttcc
     1261 agttggcaac tcccgaaacc ccggttcccg gcgagggctt gccgtaatgc gtgtcgatca
     1321 ggaaggcccg gaccccgtag cccagctggg tcgggatcga gtaatgctgg ttggggatcg
     1381 cccagccata ttcctcgttt gacattgagt tgtgggtgcc cggcaggacg acctggtcga
     1441 gcgttctcga gcagagctct accgacccgt tgcaggggcg ctcgcgggtg gcggcactgg
     1501 ccagaccggc ggtcgtgaag gtcaggacgc tgatcgctgc ggcgagccac agagcactct
     1561 ttctcccagg cattgaaacc atcatctcag gtaaccgcca ggtaaggccg gggttgcggc
     1621 caatacgatg gctttcgtgg aatccagggg agttatcaag ctggcccaga gcattctggg
     1681 aatcgcggtc cgctcgctgt tcgccctgcc cgaccggctc atggcgttga tcttcggcac
     1741 cccgcccgcc gaggctgctg ctttgcggcc ggatgcctgg gcgatgggcc gggtgatcgg
     1801 cctgatcgaa gacaggcgga ctgactggga cccggaggaa gcccgtgagg agaccgactt
     1861 ccttgcggga gcggtcagcc tcgccgtgcc ggaagtgatc agccaggact gggtcctcga
     1921 agacggacag cgcggcctgc cggcacggct cttcatcccg gaatcgaacc cccttgccgg
     1981 accgatgttg ctgcacttcc acggcggcgg ctgggtacag
//
LOCUS       DAOTIP010000401         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66177_length_2020_cov_3.151145, whole genome shotgun sequence.
ACCESSION   DAOTIP010000401 DAOTIP010000000
VERSION     DAOTIP010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66177_length_2020_cov_3.151145"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="P5138_07875"
     CDS             <1..376
                     /locus_tag="P5138_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV60533.1"
                     /translation="FDGGFGASLLARNIGIKKAKEFWFLCRHYTAEEALDMGLVNAVV
                     PVDDLEKETVAWCREMLHLSPLSLRLLKSSFNAEEDGLTGLQQLSHDATLLFYMSEEG
                     QEGRNAFQQGRKPDFDKFPKRP"
     gene            373..1254
                     /locus_tag="P5138_07880"
     CDS             373..1254
                     /locus_tag="P5138_07880"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HRV60534.1"
                     /translation="MRIWLNAARPRTLPAAVAPVFVGTAAAIYAADDFPRVLGFLAAL
                     VASILIQIGTNLANDYSDARRGADSADRLGPVRVTSAGLITPKRVLTATWIAFGLAAL
                     LGLYLAWLAGWVILVVGIVSIAAGFLYTGGPRPYGYAGLGEVFVFVFFGLVAVNGSYY
                     VQLEELAWLPFGLSVTVGLLSTAILVVNNVRDIDSDRRAEKNTLAVRLGREKTRRLYV
                     FLVLAAFFVLGLTIVLNDGPWLALIGLIAIPMARDPIAAVTGRTDGPSLNKALAGTGA
                     LLGATSLLTAIGLVLGA"
     gene            1256..>2020
                     /locus_tag="P5138_07885"
     CDS             1256..>2020
                     /locus_tag="P5138_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRV60535.1"
                     /translation="MEIGLLSVEVIPFALSFRQPYVTATGVLQRRESVLLRIRDENGI
                     TGLGEGVPMTLRGGDGLERVLDELEDWSNEPSRFPNSSPARCAVSMAVADLVAKREQI
                     PLWQYLDPAAVPRTLECNATITAGETADVVGQCEHWAADGFRVFKLKAGPAEATGLAS
                     AVRSALGPEVAIRIDANGTWGGEALSLLGELEPIGVELVEEPVTGLSELAELSRGTSI
                     PLVADESVNDASEAADARLEDACAAVTVKLSKIGGLD"
BASE COUNT          318 a          680 c          653 g          369 t
ORIGIN      
        1 cttcgacggc ggcttcggcg ccagcctgct ggcccgcaac atcggcatca agaaagccaa
       61 ggaattctgg ttcctttgcc gtcactacac ggcggaggaa gcccttgaca tgggcctggt
      121 caacgcggta gtgccggtcg acgatctcga gaaggagacg gttgcctggt gtcgcgagat
      181 gctccacctc tcccccctct cgctgcggct gctcaagtcc agcttcaacg ccgaggagga
      241 cgggctgacc ggccttcagc agctctccca tgacgccacg ctgctcttct acatgtcgga
      301 ggaaggccag gagggccgaa acgctttcca gcagggccgc aagccggact tcgacaagtt
      361 cccgaaacgt ccgtgaggat ctggctgaac gcggcgcggc cgcgtaccct ccctgccgcg
      421 gtcgcaccgg tcttcgtcgg caccgccgcc gcgatttacg cggcggatga cttccccagg
      481 gtgctcggct tcctggcggc gctggtcgcc agcatcctga tccagatcgg gaccaacctg
      541 gccaacgact actcggacgc ccggcgcgga gcggattcgg ccgaccgtct cggtccggtc
      601 agggtgacat cggcggggct gatcaccccg aagcgggtgc tgaccgcaac ctggatcgcc
      661 ttcgggcttg ccgcactcct gggtctctac ctggcctggc ttgccggctg ggtcattctc
      721 gtggtcggaa ttgtttctat cgcagccggt ttcctgtaca ccggcggccc gaggccgtat
      781 ggctacgccg gactcggcga agtcttcgtc tttgtctttt ttggccttgt cgccgtcaac
      841 ggttcctatt acgtccagct ggaagagctc gcctggctgc ccttcggcct ttcggtgacg
      901 gtcggtctgc tctcgaccgc gatcctggtc gtcaacaacg tgcgcgacat cgattcggac
      961 cggcgggcgg aaaagaacac cctggccgtc cgcctcggcc gcgaaaagac gcggcgcctt
     1021 tacgttttcc tggttctggc ggccttcttc gtgcttggtc tgaccatcgt cctgaatgac
     1081 ggcccgtggc tcgcactgat cggactgatc gcgatcccga tggcccgcga cccgattgcc
     1141 gcggtaaccg gtcgtaccga cggcccgtcg ctgaacaagg cgctcgccgg aaccggggcg
     1201 ctgctcggag ccacctcgct gctcaccgcg atcggactgg tactcggggc ctgaaatgga
     1261 gatcggactg ctcagcgtcg aggtgattcc cttcgcgctg agcttccgcc agccgtatgt
     1321 cacggccacc ggggttctgc aacgccggga atcggtgctt ctgcgcattc gcgacgagaa
     1381 cggcatcacc ggcctgggtg aaggcgtgcc catgaccctg cggggtgggg acgggctcga
     1441 gcgggtgctc gatgagctcg aagactggag caacgagccg tcccggttcc cgaactcgtc
     1501 cccggcccgt tgcgccgtga gcatggcggt ggccgacctg gtcgccaagc gggagcagat
     1561 accgctctgg cagtacctgg accctgccgc ggtacctcga accctcgaat gcaacgcgac
     1621 catcaccgcc ggcgaaaccg cggatgtagt cggtcaatgc gagcactggg ccgccgacgg
     1681 cttcagggtc ttcaagctca aggccggtcc cgcggaggcg accgggctgg cttcggcggt
     1741 tcgctcggct ctcggccccg aggtcgccat acgaattgac gcgaacggca cctggggagg
     1801 cgaagccctc tccctgctcg gtgaactgga accgatcgga gtggagctcg tcgaggaacc
     1861 cgtcacggga ctgtccgaac tggcggagct gagtcgcggc acatccatcc ccctcgtcgc
     1921 cgacgaatcc gtaaacgatg cctccgaggc cgccgacgca cggctcgagg acgcctgcgc
     1981 cgctgtgacc gtgaagctca gcaagatcgg gggcctcgat
//
LOCUS       DAOTIP010000402         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66231_length_2019_cov_1.920570, whole genome shotgun sequence.
ACCESSION   DAOTIP010000402 DAOTIP010000000
VERSION     DAOTIP010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66231_length_2019_cov_1.920570"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="P5138_07890"
     CDS             <1..1052
                     /locus_tag="P5138_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRV60536.1"
                     /translation="GKWGGWRSKQWNFLPCLSICHNSLPSNRAQSRGGMNVIRAWQNM
                     RRAGRAGAAGVKVAVIDSGIAYRKYGRRFHRAPDIGAGRFLPGYDFIDNDRLPLDLFG
                     HGTHVSMTIGEQTNNRRGLTGIAYRSKLMPVRVLDAYGDGNTADIVNGIRWATNHGAR
                     VMVMSLNFTCGLEIPPLEQALKQAWRKGVVLVGSSGNIGNETCPSLPATSPEVISVGG
                     TTESGCVASYTFRSSAIDIAAPGGGSDREDCPWKSRNRPILQLAMVAHDPSWFAIEPR
                     WVGTSMAAAHVAGAAAAVIASGVLGDKAGPGRVAERLEKTARLPAYAAGDSASGFGAG
                     IVNLGRATNPSVSTG"
     gene            complement(1083..1832)
                     /locus_tag="P5138_07895"
     CDS             complement(1083..1832)
                     /locus_tag="P5138_07895"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRV60537.1"
                     /translation="MAETDAARLAGLIKESNSVVALTGAGISVPSGIPDFRSAGSGLW
                     ENVNPMEVAHIDAWRRDPGRFWEFYGQRFGMLADKEPNQAHLALARLEQDGLLDAVLT
                     QNIDMLHQKAGTSEPIELHGTIRTSSCLSCGAREQLDRVVEMLSESEDGIPRCDCGEP
                     LKPDVVLFGEMLPEEAINRAFDLASGADLLLCVGTSLEVFPVAALPGETARAGGKVAL
                     VTQGPTPWDERAEVKLQTDVEEDLAAVLAGI"
     gene            complement(1842..2015)
                     /locus_tag="P5138_07900"
     CDS             complement(1842..2015)
                     /locus_tag="P5138_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV60538.1"
                     /translation="MDGDPADAILKVAEDLDADLIVVGNKGMTGARRFLLGSVPNKVS
                     HHAPCSVFIARTT"
BASE COUNT          335 a          686 c          675 g          323 t
ORIGIN      
        1 ggggcaagtg gggcggctgg cgcagcaaac agtggaactt ccttccctgc ctgagcatct
       61 gccacaactc cctcccttcg aaccgggccc agtcgcgcgg cggtatgaac gtgatccggg
      121 catggcagaa catgcggcgg gccgggcggg cgggagccgc cggagtgaag gtcgccgtga
      181 tcgacagtgg tatcgcctac cgcaagtacg gacgccgttt tcatcgcgcg cctgacatcg
      241 gtgccgggcg tttcctcccc ggctacgact tcatcgacaa cgaccggttg ccgctcgacc
      301 tcttcggaca cggaacccat gtgtcgatga cgatcggaga gcagacgaac aaccgtcgcg
      361 ggctgaccgg gatcgcctac cggtcgaagc tgatgccggt gcgggtactc gacgcctacg
      421 gcgacggcaa taccgcggac atcgtgaacg ggatccgctg ggcgaccaac cacggggcga
      481 gagtcatggt gatgtcgctg aacttcacct gcggcctgga gatcccgccc ctggaacagg
      541 ccctgaagca ggcctggcgc aagggggtag tcctggtcgg ctcttcaggc aacatcggaa
      601 acgaaacctg cccctcgctg cctgccacgt cgccggaagt gatctcggtt ggcggcacca
      661 cggagtccgg ctgcgttgcc agctacacgt tcaggagctc ggccattgac attgcggctc
      721 caggcggcgg cagcgaccgg gaagactgcc cctggaaatc ccgcaaccgg ccgatcctgc
      781 agctggcgat ggtcgcccat gacccgagct ggttcgcgat cgagcccagg tgggtcggta
      841 cctcgatggc cgcggcgcat gtagccgggg cggcggcggc cgtgatcgct tccggagtgc
      901 tcggcgacaa ggccggcccg ggaagagtgg cggagcggct cgagaagacc gcccgcctgc
      961 ccgcctacgc cgcgggtgat tccgcctccg gcttcggcgc cggcatagtc aacctcggac
     1021 gcgcaacaaa cccctccgtc agcaccggct gattcggctt gccggacccg tccggcccgc
     1081 tctcagattc cggccaggac cgcggccagg tcctcctcga cgtcggtctg gagtttcacc
     1141 tcggcccgct cgtcccacgg tgtcgggccc tgggtgacca gagcaacctt gcctcccgcc
     1201 cgtgcggttt ccccgggcag ggccgccaca ggaaacacct ccagagaggt gccgacgcag
     1261 agcagaaggt ccgcccccga agccagatcg aaggcccggt ttatcgcctc ctcgggaagc
     1321 atctccccga acaggacgac gtcgggcttg aggggctcgc cgcagtcgca gcgcgggatc
     1381 ccgtcctccg attcggaaag catttccacc accctgtcca gctgttcccg ggcgccgcag
     1441 ctgaggcagg acgaggtgcg gatcgtcccg tggagctcga tcggttcgga ggtaccggcc
     1501 ttctggtgga gcatgtcgat gttctgggtc aggaccgcgt ccagcagccc gtcctgctcg
     1561 agccgggcca gtgccaggtg ggcctgattc ggctccttgt cggccagcat gccgaatcgc
     1621 tggccgtaga actcccagaa ccggcccggg tcacgacgcc acgcgtcgat gtgcgcgacc
     1681 tccatcgggt tgacgttttc ccagaggccg ctgccggccg agcgaaaatc ggggatgccc
     1741 gacgggaccg agatccccgc cccggtcagg gcgacaacgg agttcgactc cttgatcagc
     1801 ccggcaagcc gggctgcgtc agtttcggcc aaggcctgcc ctcaggtggt gcgggcgatg
     1861 aagacgctgc agggagcgtg gtgggaaacc ttgttgggca ccgatcccag caggaagcga
     1921 cgtgccccgg tcatgccctt gttgcccacc acgatcaggt cggcgtcgag atcctccgca
     1981 accttgagga ttgcgtccgc cggatcaccg tccatcggg
//
LOCUS       DAOTIP010000403         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66262_length_2018_cov_3.051452, whole genome shotgun sequence.
ACCESSION   DAOTIP010000403 DAOTIP010000000
VERSION     DAOTIP010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66262_length_2018_cov_3.051452"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>2018
                     /locus_tag="P5138_07905"
     CDS             143..>2018
                     /locus_tag="P5138_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRV60539.1"
                     /translation="MEEKSARRSFLLSKGWLQAVIIVMLLGFTVLGILALRTYQEGAP
                     VPVKIVDPNGDLVFDGDDVSAGQQVFLHNGLMEYGSIFGHGAYLGPDYTADYLRRSSN
                     ISIAEKGGPPVDKDGMEIEDPDPSPMPSADVARQETINEFRRNQFDEESGTLTFTRSQ
                     AEAFERLIPYYTRFFSADNTENGLRKEAITDPEDLRNLTAFFAWTAWAAAAERPGKDY
                     SYTNNWPPEPRVDNKPTANALVWSAISLVALLGGIGLLFGAFGRYRDLGWHGREQTVV
                     SFRDPSKVTLTPGQKSTAWFFFVMAALFLIQCFVGAAVQHYRAELTSFFGFDLAAILP
                     YNLLRTWHVQLSIFWVATSFVAAGIFLAPMIARREPKGQGKLGYFLLVALAIVVFGTL
                     IGSYLGIHGVIADAATNWFGLQGFEYLDLARFWQVLLVVGLLVWVYMLFRVMRSRLRR
                     EHPGNMPWLFFLAACAIPAFYAVGLLARTSEDFTITEFWRFWVVHLWVEDFLEIFTTV
                     MVAYMFVLLGVVRERVALMVILLDIGLYSVGGVVGTMHHLYFSGTPAEHMALGAFFSA
                     IEVVPLTFLTVEAWSFLQLGANQEAKSDAAFPHRWAVMFLVAVGFWNFLGAGVFGFLV
                     N"
BASE COUNT          347 a          634 c          598 g          439 t
ORIGIN      
        1 gaatccgtag ctcccggggg agcaacaaac acgctgccag ggtcagcgaa cttgtcattg
       61 cccagatccg catatagaat ccccgaagaa gcattcgtcc cgattacaga ctcgccgcga
      121 ggcgaactgc ggaaggagat ccgtggagga gaagtctgcc cgtcgaagtt tcctgctcag
      181 caaaggctgg ttgcaggcgg tcatcatcgt catgttgctc ggcttcaccg tgctcggaat
      241 actcgcttta cgcacctacc aggaaggtgc gccggtaccc gtcaagatcg tcgatccgaa
      301 tggcgatctg gtctttgacg gtgacgatgt ttcggccggt cagcaggtct tcctgcacaa
      361 cggccttatg gaatacggct ccatcttcgg ccacggtgcc tacctcggcc cggattacac
      421 cgccgactac ctgcgccgct cctcgaacat ctcgatcgct gagaagggcg gtccacccgt
      481 cgacaaggac ggaatggaga tcgaagatcc ggacccgtcg ccgatgccca gcgccgacgt
      541 ggcgcgacag gaaacaatca acgagttccg gagaaaccag ttcgacgagg aatccggaac
      601 cctcaccttc accaggtccc aggccgaggc gttcgagagg ctgatcccct actacacccg
      661 tttcttctcg gctgacaaca ccgaaaacgg gctgcgcaag gaagcgatca cggatccaga
      721 ggatctgcgg aacctgaccg ctttcttcgc ctggaccgcc tgggcggcgg ccgcagagag
      781 gcccgggaag gactactcgt acacgaacaa ctggccgccc gagccgaggg tcgacaacaa
      841 gccgaccgcc aacgccctgg tctggtcagc gatttcactg gtcgcgctgc tcggaggcat
      901 cggactgctc ttcggcgcct tcggccgcta ccgggacctc ggttggcacg gccgcgagca
      961 gaccgtggtc tccttccgcg acccgtcgaa ggtcaccttg actccgggcc agaagtcgac
     1021 cgcatggttc ttcttcgtaa tggcggcgtt attcttgatc cagtgtttcg tcggtgccgc
     1081 cgtgcagcat taccgggccg agctgaccag cttcttcggc tttgacctgg cggcaatcct
     1141 gccgtacaac ctgctccgga cctggcatgt ccagctctcg atcttctggg ttgccacttc
     1201 cttcgtcgcg gccggcatct tcctcgcgcc gatgattgca cggcgcgaac caaaggggca
     1261 gggaaagttg ggctacttcc tgctcgttgc gctggcaatc gtcgtcttcg gcaccctgat
     1321 cggttcctac ctcgggatcc atggggtgat cgccgatgca gccaccaact ggttcggtct
     1381 tcagggcttt gagtacctcg atcttgcccg cttctggcag gttctgcttg tggtcggtct
     1441 tctggtctgg gtatacatgc tcttccgggt catgcgatcc cggctgaggc gtgaacaccc
     1501 tggcaacatg ccctggttgt tcttcctggc ggcctgcgcg atcccggcct tctacgcggt
     1561 cggtctgctg gcccggacct cggaggactt cacaatcacc gagttctggc gcttctgggt
     1621 ggtccatctg tgggtagagg acttcctgga aatcttcacc accgtgatgg tcgcctacat
     1681 gttcgtcctg ctcggggtcg tgcgggagcg ggtcgcacta atggtgatcc tgctggatat
     1741 cggcctttat tcggttggcg gcgtggtagg caccatgcac cacctgtact tctcgggaac
     1801 ccccgccgag cacatggcgc tcggtgcctt cttctcggcg atagaagtgg tgccgctcac
     1861 atttctcacg gtcgaagctt ggtcgtttct ccaacttgga gcgaaccagg aggcgaaatc
     1921 cgatgccgcc ttcccgcacc gatgggcagt gatgttcctg gtcgcggtcg ggttctggaa
     1981 ttttctcggc gccggtgtct tcgggttcct tgtcaacc
//
LOCUS       DAOTIP010000404         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66269_length_2018_cov_2.775853, whole genome shotgun sequence.
ACCESSION   DAOTIP010000404 DAOTIP010000000
VERSION     DAOTIP010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66269_length_2018_cov_2.775853"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="P5138_07910"
     CDS             <1..318
                     /locus_tag="P5138_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60540.1"
                     /translation="REDQTAVLLARLEAGEIDRMPLTTSVAAVSVGMVDGQPLCDLDF
                     PEDSRAEVDANVVMTGDRGLVEVQATAERTPLSRASLDEMLALAEAGIDRLREFQMAA
                     VGE"
     gene            326..910
                     /gene="rdgB"
                     /locus_tag="P5138_07915"
     CDS             326..910
                     /gene="rdgB"
                     /locus_tag="P5138_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRV60541.1"
                     /translation="MILATRNLHKVRELDEILPAAGLRPLPDEVESPPETGVTFEENA
                     LIKARSAREATGEAVIADDSGLEVDALGGRPGVYSARYAGEGATDGENLGKVLSDLAA
                     ANGGDQSGSARYVCVIALVDDSGEHLFRGTCEGRIVRQPRGDGGFGYDPAFVPDATGP
                     DDQRTMAELSPEEKNAISHRGAAAQQLADHLADV"
     gene            complement(971..1141)
                     /locus_tag="P5138_07920"
     CDS             complement(971..1141)
                     /locus_tag="P5138_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60542.1"
                     /translation="MSLAQASWITVVAICAIATAVTAIAGYAGYAATIAAVGVAASVN
                     LLPHHGGQPTED"
     gene            1162..1590
                     /locus_tag="P5138_07925"
     CDS             1162..1590
                     /locus_tag="P5138_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60543.1"
                     /translation="MADVTGKSDESGGNALASAAGRVLENLAELEPELVAAGIFDAAR
                     EPVAVASGSSAWASEAAVLLDAVEAGSGDEKLDSAHIACEGGEAFVVSESGLSLVAVT
                     GRFVLASLTSYDMRMALRDAVRSAGLNPASEAENGGNVDA"
     gene            1583..1786
                     /locus_tag="P5138_07930"
     CDS             1583..1786
                     /locus_tag="P5138_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60544.1"
                     /translation="MLKPHRVVLDKVSSYTDEAAAFMRRRRHHRREFVRIRFQSGAEA
                     DLPEGTPEADRIAESARRLIELG"
BASE COUNT          343 a          660 c          700 g          315 t
ORIGIN      
        1 cgcgaggacc agacggcggt gctgctggcg cgcctcgaag ccggcgagat cgatcggatg
       61 cccctgacca cctccgtggc cgcggtcagc gtcggcatgg tcgatggcca gccactttgc
      121 gaccttgact tcccggagga ctccagggcc gaggtcgacg ccaacgtcgt gatgaccggt
      181 gaccgtggcc tggtcgaggt tcaggcaacg gcggagcgaa cccctctttc ccgcgcctcg
      241 ctcgacgaga tgctggctct ggccgaagcg ggcatcgaca ggctgcggga attccagatg
      301 gccgcagtcg gcgagtaagg gtcgcttgat cctcgccacc cgcaacctgc acaaggtgcg
      361 cgaactcgac gagatccttc ccgcggccgg cctgcgtcca ttgcccgacg aggtggaatc
      421 gccgccggag accggagtga ccttcgagga gaacgccctg atcaaggccc gctcggcccg
      481 cgaggcgacc ggggaggcag tgatcgccga tgattccggc ctggaagtcg acgctctcgg
      541 agggcgaccc ggggtctatt cggcccgtta cgccggtgaa ggcgccaccg acggggagaa
      601 cctgggaaag gtcctgtcgg atctggccgc agccaacggc ggggaccagt ccggcagcgc
      661 acgctacgtc tgcgtgatcg ccctggtcga cgactccgga gagcatctgt tccgcggcac
      721 ctgcgagggc cggattgtcc ggcagccaag gggggacggc ggcttcggct atgaccccgc
      781 cttcgttccc gatgccaccg gacccgacga tcagcggacc atggccgaac tgagtccgga
      841 agaaaagaac gcgatcagtc accgcggcgc cgccgcgcag cagctggccg atcacctcgc
      901 cgacgtgtag atacccaagg gtctttgctc gcccgcccgg gcccggaacc gccgggcggt
      961 ccggggcgat tcagtcttcg gtcggctggc cgccgtggtg gggcaggagg ttcaccgagg
     1021 cggcgacacc cacggccgcg atcgtcgccg cgtaaccggc atatccggcg atggccgtga
     1081 ccgcggtggc gatggcgcag atcgccacga ccgttatcca ggatgcctgg gcaagtgaca
     1141 tggccggtat gttgctacac cgtggcggat gtgaccggca agagtgacga atcgggcggg
     1201 aacgccctgg cttcggcggc gggcagggtg ctcgaaaacc tggccgaact ggagccggag
     1261 ctcgtggctg ccggaatctt cgacgcggct cgcgaaccgg tagccgtcgc ctcgggttcg
     1321 tctgcctggg caagcgaagc cgcggtcctt ctcgatgctg tcgaggccgg ttccggagac
     1381 gagaaactcg attccgccca catcgcctgc gagggcggcg aggccttcgt ggtcagcgaa
     1441 tccgggctgt ccctggtcgc cgtcaccggt cgcttcgtcc tggcctccct cacctcctac
     1501 gacatgcgga tggcacttcg ggacgccgtt cgatcggcgg ggctgaatcc cgcttctgaa
     1561 gccgaaaacg gaggtaacgt ggatgcttaa gccgcatcgc gtggtactcg acaaggtcag
     1621 ctcctacacg gacgaggcgg ccgccttcat gcgcaggcgg cgccatcacc ggcgcgaatt
     1681 cgtgcgaatc cgcttccaga gcggagccga ggccgatctg ccggaaggga ccccggaggc
     1741 ggaccggatc gccgagtcag cccggcgcct tatcgaactc ggctgattcc tgcagtccgg
     1801 tgttgccgcc gaaggcggcg gaaatggcat ccgcaagcgg tgttacggtt ctcccattcc
     1861 ctaaaccgag gaggattact gacgtgacca aagcagagtt tcttgagaag gtcgccggtg
     1921 acgagcgcat cgggagcaag aaggccgcgg ccgacgccgt cgacgccgtt ctggacgcga
     1981 tcaccgacac cctgaccgcg ggtggcgagg tcaacttc
//
LOCUS       DAOTIP010000405         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66305_length_2017_cov_3.077472, whole genome shotgun sequence.
ACCESSION   DAOTIP010000405 DAOTIP010000000
VERSION     DAOTIP010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66305_length_2017_cov_3.077472"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5138_07935"
     CDS             complement(<1..220)
                     /locus_tag="P5138_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60545.1"
                     /translation="MESLRSPRTASPRRVVIVTFPGLQPLDVVGPAEVFAGASDLTAA
                     RVEGEATNPAYEVEVVAEEAAPLSGRTSG"
     gene            complement(796..1251)
                     /locus_tag="P5138_07940"
     CDS             complement(796..1251)
                     /locus_tag="P5138_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60546.1"
                     /translation="MSLCFVDPADAIHVISESVGREARRQILGEALRAWVDEIPEEEL
                     EAQYARALADLDQDDLSLLAAWHGSMDLGQPVPNRDFLMGAFGSLGEHRREALKLAAG
                     FRGEEVLEDGLTEELALETVVGWLSPERLLDLANEAVELYVWDRIGSDN"
     gene            1425..>2017
                     /locus_tag="P5138_07945"
     CDS             1425..>2017
                     /locus_tag="P5138_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60547.1"
                     /translation="MPLTIIKGPPNSGRTELVRERFESRIADDPVLVVPSTDDIFGWE
                     RRLTRGSGAFLGGTVVHFKDLIELILGDGSAARSYEKGSDVACPLRRRALATDAIHAG
                     WPAIAGRLEDQPGLVDSALQVIDEFRAALNSPKEFDDPAISDLAGPAALVLLRGVQLR
                     HPAHRPVRGRRQQLLELVEAGHRRVLPHRQQRLHLRLL"
BASE COUNT          380 a          681 c          631 g          325 t
ORIGIN      
        1 agccgctggt ccgaccgctc aggggcgccg cctcttccgc caccacttcc acctcgtagg
       61 ccgggttcgt cgcttcacct tcgaccctgg ctgcggtcag gtcgctggcg ccggcaaaga
      121 cctcggccgg gccgaccacg tcaagcggct gaagacccgg aaaagtgacg atgacgacgc
      181 gtcggggtga agcggtgcgg ggcgaacgaa gcgattccat gcacccatag tgcccgtatc
      241 aacgcttggc cgcaaggacc aataccagac aattcctgcc actcccctcc ccggcccagc
      301 cccagcccaa aggtgccgca catccagccc catcacggtg ccaaacctgc gtcacctgcc
      361 ccgaaaatcc gacccactgg cacctccgga actgattcgc aagtctcgtg ggtcggattt
      421 tcggcccgac tgcggagaat ccgacccacc tgggcggtgc agggcaggtg aaggggaagt
      481 tgaaggaacc cagggtccgg gctggctccg gggccgaatt cgcgtcctgg ttcagggcgg
      541 caccgtcccc cgagtcacgg tcgggaatcg atcgagatgc tgtttgtcgg cgatatttgc
      601 cgacaaacag catctcgcga aatgccaaca ccaccaaccc tgagcgggtc gtgcacaggg
      661 gcctccagcc gttcgctggc aaggagacag gccggaaatg accgcgatag tacgcgggta
      721 cgtgagctgt cgtttccggc cgccgacgaa gtcagcgtgc ggctggtggg tcctgggcgc
      781 gttgcccggg ggcttctagt tgtcggagcc gattcgatcc cagacgtaca actcgaccgc
      841 ctcgttggcg aggtcgagga gacgttccgg ggagagccag ccgaccaccg tttcgagggc
      901 gagctcctcg gtcaggccgt cttcgaggac ttcttccccg cggaagccgg ccgccagctt
      961 cagggcctcc cggcggtgct cgccgagcga accgaaggcg cccatcagga agtcgcggtt
     1021 gggaaccggc tggcccaggt ccatcgagcc gtgccaggcg gcgagcaggg agaggtcatc
     1081 ctggtcgagg tcggcgaggg cacgggcgta ctgggcctcg agttcctctt cggggatctc
     1141 gtccacccat gcacgcagag cctccccgag gatctggcga cgggcctcgc ggccaaccga
     1201 ttccgaaatg acgtgaatcg catcagccgg atcgacgaaa cagagtgaca tgttgggccg
     1261 cctcggggtt gcttactcgg acaaccgtga agatagagat gccacaggac gcaaaaaatg
     1321 ttgcagaacc tcgcaatttg cgggagggca agcggaaaac gaacctttcc gcaaagccgg
     1381 cgatgtcaga aaacgcccgc cgctcccgac cataaagttg ccccgtgccc ctgactatca
     1441 tcaaaggccc cccgaactcc ggccggaccg aactcgtccg cgagcgcttc gagtcccgga
     1501 tcgccgacga tcccgtcctc gtcgtgccct ccaccgacga catcttcggc tgggagcgac
     1561 gcctgacccg cggaagcgga gccttcctcg ggggaacggt ggtccacttc aaggacctga
     1621 tcgaactcat cctcggagat ggatcggcgg cacgcagcta tgagaagggc agcgacgtcg
     1681 cctgccccct tcgtcgtcgc gcgctcgcta ccgacgccat ccacgcgggc tggcctgcga
     1741 tcgccggccg gctggaagat cagcccgggc tggtcgactc ggcgcttcag gtaatcgacg
     1801 agttccgggc cgccctgaat tccccgaagg agttcgatga tcctgccatt tccgatctgg
     1861 ccggacctgc cgcgctcgtc ctgctacgcg gagtacaact gcgccacccg gcgcaccgcc
     1921 ctgtacgagg gcggcgacaa caactactgg aactggtcga agccgggcat cgacgagttc
     1981 ttccgcaccg gcaacaacga cttcatctac gacttct
//
LOCUS       DAOTIP010000406         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66311_length_2017_cov_2.722732, whole genome shotgun sequence.
ACCESSION   DAOTIP010000406 DAOTIP010000000
VERSION     DAOTIP010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66311_length_2017_cov_2.722732"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="P5138_07950"
     CDS             complement(<1..330)
                     /locus_tag="P5138_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60548.1"
                     /translation="MGPANGLSAAYGIARVGFGIFASCAPQSLGRTWVGEDSESPGAG
                     VILRALGFRDIALGAGTTQAALAGDPRGWLAVSMLSDLGDVAATLIGRDRIESRGVVI
                     TSTVAGAG"
     gene            383..1123
                     /locus_tag="P5138_07955"
     CDS             383..1123
                     /locus_tag="P5138_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRV60549.1"
                     /translation="MPITEKVTLHALPPSHPCKAVGAALDYKGIEYEWVNLDFGKHNE
                     QITELYGEGKTRVPGLTIGEERVHGSTNIFKRLEELVPELSLYPEGIADEVREAELWG
                     DRVLQDLGRRIPWGGIYFRPEMMGKMNGIEELDPAGTDFAMKFVRATWKYHNISCVQI
                     AEDLEKFPGMIDEIEGMAADGLVDGENPTAADLQIGSSIWVIGTISDVKPVIEGSAAE
                     RIAERYFGEIPEMIPAGALPASWIKLRD"
     gene            complement(1051..>2017)
                     /locus_tag="P5138_07960"
     CDS             complement(1051..>2017)
                     /locus_tag="P5138_07960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRV60550.1"
                     /translation="RFRFILWPFQKLGPLMPRTTRRRGKKQISAHYDLGNDLFSLFLD
                     ESMNYSAALYRGAEDESLEAAQIAKMNQIADQLDLDRDSHLLEIGTGWGGLSIHLAQR
                     SGCRVTTTTISKEQAALARERIAEAGLEDRIEVLETDYRDLTGTYDRLVSIEMIEAVG
                     WRDFPTYFAKCSELLEPDGSMLLQSITIDDDGYELEKASRSFISRYIFPGGCLPSMAE
                     IRRCLDGFTDLTVAWADELRLDYSKTLAEWRRRFNSATARLEQLGYDERFRRLWVLYL
                     SLAEAGFTSGRNSDFQLLLAKPQSRSLIQLAGKAPAGIISGISPK"
BASE COUNT          385 a          631 c          668 g          333 t
ORIGIN      
        1 cccggccccg gccaccgtcg aagtgatgac gacaccgcgc gactcgatcc gatcgcggcc
       61 aatcaacgtt gcggcgacat caccgagatc ggacagcatc gatacggcca gccatccgcg
      121 cgggtcaccg gcaagtgccg cctgggtggt ccccgccccg agggcgatgt cacgaaaacc
      181 gagtgcgcgg aggatcactc ccgcgcccgg actttcggaa tcctctccga cccaggttcg
      241 accgaggctt tgcggagcgc aggaggcgaa gattccgaaa ccgaccctgg cgattccgta
      301 ggcggcgctg agtccgttgg ctgggcccat gggcgaaacc tacccggttg ccatgcggtt
      361 caccgggcca taaagtctgg gcatgcccat caccgagaag gtaaccctgc atgcacttcc
      421 tccctcccac ccctgcaagg cggtcggggc cgcactcgat tacaagggga tcgagtacga
      481 gtgggtgaac ctcgacttcg ggaagcacaa cgaacagatc accgagcttt acggggaagg
      541 caagacccgg gtccccggac tgacaatcgg cgaggagcgc gttcacggat cgaccaacat
      601 cttcaagcgg ctggaggagc tcgtccccga actgtccctc tatccggaag gcatcgcgga
      661 tgaggtccgg gaggcggagc tctggggaga ccgggtgctt caggatctgg gccgccgcat
      721 cccctggggc ggcatttact tccggccgga aatgatgggc aaaatgaacg gcatcgagga
      781 gctggatccg gccggcaccg atttcgcgat gaagttcgtc cgggccacct ggaagtacca
      841 caacatcagc tgcgttcaga tcgccgagga cctggagaag ttcccgggca tgatcgacga
      901 aatcgagggg atggccgccg acgggctggt tgacggcgag aacccgaccg ccgctgacct
      961 gcagatcggc tcgtcgatct gggtgatcgg cacgatctcc gacgtcaagc cggtgatcga
     1021 gggaagcgca gccgagcgga tcgccgagcg ttacttcggg gagatccccg aaatgatccc
     1081 ggccggcgcc ttgccggcca gctggatcaa gctgcgcgac tgaggcttcg ccagcagcag
     1141 ctggaaatcg ctgttccggc ccgaggtgaa ccccgcctcg gcgaggctga ggtagagaac
     1201 ccaaaggcga cggaaccgtt cgtcgtagcc gagctgctcg agcctggccg tggccgagtt
     1261 gaaccgccgc cgccattcgg ccagcgtctt cgagtaatcg agtctcagct cgtcggccca
     1321 ggcgacggtc aggtcggtga agccgtccag gcatcgccgg atctcggcca tcgaaggcag
     1381 acagccgccg gggaagatgt agcggctgat gaagctgcgg gaggcctttt cgagctcgta
     1441 gccgtcgtca tcgatcgtga tcgactgaag cagcatcgac ccgtcgggct cgagcagctc
     1501 cgagcacttg gcgaaatagg tcgggaaatc ccgccagccg accgcttcga tcatctcgat
     1561 cgagacgagg cggtcatagg tgccggtcag atcacggtag tcggtctcga gaacctcgat
     1621 ccggtcctcg agcccggctt cggcaattcg ctcgcgggcc agggcggctt gttccttcga
     1681 gatcgtcgtg gtggtcaccc ggcaaccgga ccgctgcgcc aggtgaatcg agaggcctcc
     1741 ccagccggtc ccgatctcga gcaggtggct gtcccggtca aggtccagct ggtcggcgat
     1801 ctggttcatc ttggcgatct gcgccgcttc gagggattcg tcctccgctc ccctgtagag
     1861 cgccgccgag tagttcatcg actcgtccag gaagagcgag aagagatcgt tgccgaggtc
     1921 gtaatgcgcg gagatctgct tcttgccgcg ccgccgggtc gtccggggca taagcggacc
     1981 cagtttctgg aagggccaga ggatgaaacg gaaacgg
//
LOCUS       DAOTIP010000407         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66387_length_2015_cov_3.729592, whole genome shotgun sequence.
ACCESSION   DAOTIP010000407 DAOTIP010000000
VERSION     DAOTIP010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66387_length_2015_cov_3.729592"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="P5138_07965"
     CDS             complement(<1..985)
                     /locus_tag="P5138_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRV60551.1"
                     /translation="MSETPSGMAEQILAFCEDLRSEDVAIGTSEIQDAFKALEIIPWT
                     EPVDFRESLATTLAKSPKDREIFDLVFDRHFFRATEAASLEYAPEAGEGRKAALSDDV
                     REELSMEALAEAIRQAIAEGDESAMNELARLAIEAFGRQGESSGVVGVDMQRIRRGLG
                     LSPSSDTSGDGEGEGERLDRDQIARFERQLRRELQRQQIQRQGQLPPARPLSDLNRSL
                     PMRGAQDLAAVHKAVAQMKRRLATLGHDPRGAKRGRTVDVRRTMRSSLETGGVPLNLK
                     YRPRRPRKPEIYVLCDVSTSVSSASTFFLSVLHALHDSFRKLRSFVFIERIS"
     gene            complement(1002..1931)
                     /locus_tag="P5138_07970"
     CDS             complement(1002..1931)
                     /locus_tag="P5138_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRV60552.1"
                     /translation="MAVTPQAAPDSPGEVADALREASYLADESTALIAFLAQRLGKPV
                     LVEGPAGVGKTELAKALSRATGRELVRLQCYEGLDEAKALYEWNYRKQLLQIQATASA
                     GDGDSAPVGMGEIFTEQFLLERPLMKAIASPDPVVLLIDEIDKTDQEFEAMLLELLSD
                     FQITIPEMGQISATTHPIVLLTSNNSRELTEALKRRCLYLWLDYPSREREIEIIRLHA
                     PDIDEALAGRLVDVVDMVRELDLKKPPSIAESIDWARTLILMGADDIDEKTFRDSLSI
                     IIKHRTDLDLVAEKIVPRLGGASSNGSSSNGSG"
BASE COUNT          306 a          652 c          699 g          358 t
ORIGIN      
        1 ccgagatccg ttcgatgaag acgaagctcc gcagcttgcg aaacgagtca tggagagcat
       61 gcaggaccga gaggaagaag gtcgaggcgg aactgaccga ggtcgagacg tcgcagagca
      121 cgtagatctc cggcttgcgt ggccggcgcg gccggtactt caggttgagc ggtaccccgc
      181 cggtctcgag cgaggatcgc atggtccggc gcacgtccac ggtgcggccc cgcttggctc
      241 cgcgcggatc gtggccgagg gtggccagtc ggcgcttcat ctgggcgacc gccttgtgga
      301 ccgcggcgag atcctgggca ccgcgcatcg gcagcgaacg gttcaggtcg gagagcggcc
      361 gggcaggcgg caactggccc tgacgctgga tctgctggcg ctgcagctcc cgccgcagct
      421 gccgttcgaa ccgggcgatc tggtcccggt caagccgttc gccttcacct tcgccgtcgc
      481 cgctggtgtc cgaggagggt gacaggccga gtccgcggcg gatccgctgc atgtcgacgc
      541 cgaccacgcc cgaactctcg ccctgcctgc cgaaagcctc gatcgccagg cgggccagct
      601 cgttcatggc cgattcgtcg ccctcggcga tcgcctggcg gatcgcctcg gccagcgcct
      661 ccatgctgag ctcctcccgg acgtcgtcgg aaagggcggc cttccggccc tcgcccgcct
      721 ccggtgcgta ctccagcgat gccgcctcgg tcgcccggaa gaaatggcgg tcgaagacga
      781 ggtcgaagat ctcccggtcc ttcggcgact tggccagggt ggtcgccagg gattcgcgga
      841 agtcgaccgg ctcggtccag gggatgatct ccagggcctt gaacgcgtcc tggatctccg
      901 aggtgccgat cgcgacatcc tccgagcgga gatcctcaca gaaggcgagg atctgctcgg
      961 ccattcctga gggcgtctcg ctcatcctga atacccgtct gtcagccgct gccgttggag
     1021 gaggagccgt tgctcgaagc gccgcccagc cggggcacga tcttctcggc gaccaggtcg
     1081 aggtcggtgc ggtgcttgat gatgatcgac agcgaatcgc ggaaggtctt ctcgtcgatg
     1141 tcgtcggccc ccatcaggat cagggtccgg gcccagtcga tcgactccgc gatcgaaggc
     1201 ggcttcttca ggtcgagctc gcggaccatg tcgaccacgt cgaccagcct tccggccaga
     1261 gcctcgtcga tatccggggc gtggagccgg atgatctcga tctcccgctc gcgtgagggg
     1321 tagtcgagcc agaggtaaag acagcgtcgt ttcagggcct cggtcagctc ccgcgaattg
     1381 ttcgaggtga gcagcacgat cgggtgggtg gtggcactga tctggcccat ctcggggatc
     1441 gtgatctgga agtcggaaag cagctcgagc agcatcgcct cgaactcctg gtcggtcttg
     1501 tcgatctcgt cgatcagcag gacgaccgga tccgggctgg cgattgcctt catcaggggc
     1561 cgctccagca ggaactgttc ggtgaagatc tcgcccatgc cgaccggggc cgaatcaccg
     1621 tcgccggccg atgcggtggc ctggatctgg agcagctgct tgcggtagtt ccactcgtag
     1681 agcgccttgg cctcgtcgag cccttcgtag cactggagcc ggaccagctc ccggccggtc
     1741 gcccttgaaa gtgccttggc cagttcggtc ttgccgactc cggccgggcc ttcgaccagc
     1801 accggcttgc ccaggcgctg ggcgaggaag gcgatcagcg ctgttgactc gtcggccagg
     1861 tatgaggcct cgcgcagggc atctgcgacc tcgccggggc tatcgggggc ggcttgaggg
     1921 gttactgcca tgtctggaca atacgccggt cagtccagcg ggatgtccgt gacccgcggg
     1981 gccggcggct cttccggttc gtcccggcgg ggcat
//
LOCUS       DAOTIP010000408         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66391_length_2015_cov_3.407143, whole genome shotgun sequence.
ACCESSION   DAOTIP010000408 DAOTIP010000000
VERSION     DAOTIP010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66391_length_2015_cov_3.407143"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="P5138_07975"
     CDS             complement(<1..108)
                     /locus_tag="P5138_07975"
                     /inference="COORDINATES: protein motif:HMM:NF037053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60553.1"
                     /translation="MKEGATEDQIAHVVERVEEIGCSAHISEGELLTVIG"
     gene            complement(398..1510)
                     /gene="hisC"
                     /locus_tag="P5138_07980"
     CDS             complement(398..1510)
                     /gene="hisC"
                     /locus_tag="P5138_07980"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV60554.1"
                     /translation="MTVRFNSHLTSIPGYSPGVPKGHSAEDVASSNLAQLASNESPFA
                     PLPEVVEAITKAAGAMNRYPDPAATLLRQRIADRHEIEPGQVSVANGSCEFLLAAAEA
                     LLEPGAEIVYAWPSFSMYPNMAAATGAREIRVPLKEDQSHDLEAMLAEITAATQIVIV
                     CNPNNPTGTYIPFPEIASFVERVPDHVQIILDEAYIEFQVYDDPAISIDLLKRFPNLV
                     LLRTFSKTHSLAGLRVGYALGSPAFKAAVDVVRQPFSVNALAQAAAVEALEHGDVIDR
                     QIETQIAERVRVESGVADLGLMATDSQANFSWISLGENGAEIEDAVVGSLAEANIVVR
                     AGKLLGGPGWLRVSYGTPEENDRFVAALGDAMRIHG"
     gene            complement(1537..1923)
                     /gene="aroH"
                     /locus_tag="P5138_07985"
     CDS             complement(1537..1923)
                     /gene="aroH"
                     /locus_tag="P5138_07985"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRV60555.1"
                     /translation="MTEPEAESMRVTAIRGAVQAENDADSILAATEHLMREIIEVNGL
                     EPDRMISALFTTTEDLDAEFPAVAARRMGLNEVPLMCAREIPVPGAMTGVIRVMLHTY
                     LPSGAKARHVYLGETRNLRADLDAAQ"
BASE COUNT          357 a          627 c          635 g          396 t
ORIGIN      
        1 cccgatcacg gtgaggagtt caccttcgga gatatgtgcc gagcagccga tctcctcgac
       61 ccgttccact acgtgggcga tctggtcttc ggtcgccccc tctttcatca cgatcatcat
      121 gtctgcttca aacctttcct ttactgcctc aagactttac tttcgtccgg cccgaacggc
      181 agcggacgac accgactgac acggcgcccg gcggcttttc agatcagttg cgggcagcta
      241 cccgcgacac cggacggacg cctagataaa tcgccaatac gaatagaagg cagcaccgaa
      301 ggccccgctg gagcggagtg ccgtgcccgg aagggcgatg cggctgtcgg tctgctgctg
      361 cattgggtga ttttttatca ggtagcggtg gtgaacgcta gccgtgaatt ctcatcgcat
      421 cgccgagtgc ggcaacaaat cggtcgtttt cttcaggcgt gccgtaagag acccgtagcc
      481 atcccgggcc acccagcagc tttcccgccc ggaccacgat gttagcttcg gccagcgatc
      541 cgaccaccgc atcctcgatc tccgccccgt tctcgccgag cgatatccag gagaagttgg
      601 cttggctgtc ggtcgccatc aggcccaggt cggccacccc ggactcgacc cgcactcgct
      661 ccgcgatctg ggtttcgatc tgccgatcaa tcacgtctcc gtgttcgagc gcctcgaccg
      721 cagcagcctg ggccagggcg ttgacgctga aaggctggcg cactacgtcc acggccgcct
      781 tgaaggcggg tgagccgagt gcgtaaccaa cccggaggcc agccagggaa tgagtcttgc
      841 tgaaggtgcg aagcaggacc aggttcggaa accgcttcag caggtcgatc gaaattgccg
      901 ggtcgtcgta gacctgaaac tcgatgtagg cctcgtcgag aatgatctgg acatggtcgg
      961 gcacccgctc gacgaaactc gcgatttcag ggaacgggat gtaggtgccg gtcggattgt
     1021 tcggattgca gacgatcacg atctgggtcg cggcggtgat ctcggccagc atcgcctcga
     1081 gatcgtggct ctggtcttcc ttcagcggaa cccggatttc ccgggcaccg gtcgccgccg
     1141 ccatgttcgg gtacatcgaa aacgacggcc aggcgtagac gatttccgcc cccggctcga
     1201 gcagcgcttc agccgcggcc aggaggaact cgcaggatcc gttggcgacc gagacctgtc
     1261 cgggttcaat ttcgtgacgg tcggcgatcc gttggcggag tagggtcgcg gccgggtccg
     1321 ggtagcggtt catcgccccg gccgccttcg tgattgcctc aaccacttcc gggagcggag
     1381 cgaatgggga ttcattcgaa gcgagctggg ccagattgga gctggctaca tcctcggcgc
     1441 tgtggccttt cggcacgccc gggctgtaac cgggaatcga ggtgagatgg gagttgaagc
     1501 gaacggtcat ggcagatccg gggtcgggga cggtgattac tgggcggcgt cgaggtcggc
     1561 ccgcagattg cgggtttccc caaggtaaac atgccgggct ttcgcgccag acggaaggta
     1621 ggtgtggagc atcaccctga tgaccccggt catggcaccg ggtaccggaa tttcgcgggc
     1681 gcacatcagt ggcacctcgt tgagacccat gcgacgggcg gcaacagccg ggaactcggc
     1741 atcgaggtcc tcggtcgtgg tgaacagggc gctgatcatc cggtcgggtt caagcccgtt
     1801 gacctcgatg atctcccgca tcagatgttc ggtggcagcc aggatcgaat cggcgtcgtt
     1861 ctcagcctgc accgccccgc gtatcgcggt gaccctcatg ctctctgctt ccggctcagt
     1921 catcgcctgc atctttccag aggcgctcca cctcgcgttc atcgagcagc ctcgactttc
     1981 cggtacccaa cctgcccagg cggagtgatc cgagt
//
LOCUS       DAOTIP010000409         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66615_length_2010_cov_7.750895, whole genome shotgun sequence.
ACCESSION   DAOTIP010000409 DAOTIP010000000
VERSION     DAOTIP010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66615_length_2010_cov_7.750895"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..424)
                     /locus_tag="P5138_07990"
     CDS             complement(155..424)
                     /locus_tag="P5138_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60556.1"
                     /translation="MVWIALYGITAIAVAVAAFLFAEWVREPTSSDLNRPGTLAALTG
                     LLWPVVAAGVVELGLLAVVRSRLRSHSPGLVPQPVRAPGRQPVAL"
     gene            690..>2010
                     /locus_tag="P5138_07995"
     CDS             690..>2010
                     /locus_tag="P5138_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRV60557.1"
                     /translation="MPAAQPYQDIPRPANRVPLLGDALHIDAAHPTRSLMDLAGELGP
                     IYSLVAPGQDVVVVSGGELVAECLDETRFEKNVPTELIVMREIVGDAVFTAWTEEPAW
                     KKAHNILLPAFAPQAIRGYTEKMADIADQLVMKWARLNPGDPVDICADMTKLTFDTIC
                     LVCFSYRPGSFYRQDMPPIVTALEEAITECVNRPSRLPGQDIYEAIKGRSRFKEHLSF
                     LNNLCDKLIDDRIRSADVGRNGDVLDLMLTQADKDTGQKLSRLSIRQQMLTFLAAGYD
                     TTSGMLMWTIYYLLKNPAALAKCYAEVDEVFGEDLSVLPDAAQIPKLQYLLQCMKEAL
                     RLWPVGPGFQVRPLADEVLGGRYRIKKGQPILILTPQIHRDPNHFPDPECYDPDRFAP
                     ELEATRPGWAWIPFGNGQRACIGRHFSFFESQLLLGLILQRFRLLDSF"
BASE COUNT          406 a          672 c          620 g          312 t
ORIGIN      
        1 tgtcgggaac ctgcggagcc cagccgggca ccgaatacag accccagtga atgaaaatcc
       61 cgagtttggc gtcgtcgtac cagtccggaa ggggatgcgt cgaaacggat ccccaggtgg
      121 gggcgtacat ccgaaaaggg taagcccccc ggcctcagag agcgaccggc tgcctcccgg
      181 gcgcccgaac cggctgcggc acaaggcccg ggctatggct ccgcagccgg gaacgcacca
      241 ccgcgagcag gcccagttcc accactcccg cggcgaccac gggccagagg agtccggtga
      301 gggccgccag ggtcccgggc cggttgaggt cgctcgaggt cggttcgcgc acccattccg
      361 cgaacaggaa cgctgcgacg gcgacggcga tcgccgtgat tccgtagaga gcaatccaga
      421 ccatgcggat ctccgaaagt tagatgactt aacggtcaat ttcgacggta cgcccgccat
      481 acagaaagtc gaatcacgaa gccatcttca tatcaaaccg atacaaagct gtgcccttgg
      541 ccgttagatg ccgcggcccc cttccgaact gcgacactga tcgacggcac gtcaagcaga
      601 ccgggcaaaa gcccgatggc gttggcggcc gcgatcgcgc cgcctctgac gattcagcgc
      661 aagcgaaaca ggaaggtggc cacgtcgcga tgccagccgc acagccctac caggacattc
      721 cccgaccggc caaccgagtt ccgctgctgg gcgacgccct gcacatcgac gccgcccatc
      781 ccacgcgcag cctgatggac ctggccgggg agctcgggcc gatctactcg ctggtggcac
      841 ccggccagga cgtcgtcgtg gtctccggcg gcgagctggt ggcggaatgc ctcgacgaga
      901 cacgattcga gaagaacgtc ccgaccgaac tgatcgtgat gcgggagatc gtcggcgacg
      961 ccgtcttcac cgcctggacc gaggagcccg cctggaagaa ggcacacaac atcttgttgc
     1021 cggctttcgc cccgcaggcg atcaggggct acaccgagaa gatggccgat atcgccgacc
     1081 agctggtgat gaagtgggcc cgcctcaacc ccggcgaccc ggtggacatc tgcgccgaca
     1141 tgaccaagct gaccttcgac accatctgcc tggtctgctt cagctaccgg ccgggttcgt
     1201 tttaccggca ggacatgccg cccatcgtga cagcgctgga ggaggcgatc accgaatgcg
     1261 tgaaccggcc cagccggctg cccggccagg acatctacga ggcgatcaag ggacgcagca
     1321 ggttcaagga gcacctcagc ttcctgaaca acctctgcga caagctcatc gacgaccgga
     1381 tccgcagcgc cgacgtcggc aggaacggcg acgtcctgga cctgatgctg acgcaggccg
     1441 acaaggacac cggacagaag ctcagccggc tgagcatccg ccagcagatg ctgaccttcc
     1501 tggccgccgg ctacgacacc accagcggaa tgctgatgtg gacgatctat tacctgctga
     1561 aaaaccctgc agccctagcg aaatgctacg ccgaggtcga tgaggtcttc ggggaggacc
     1621 tgtccgtgct gccggacgcg gcgcagatcc ccaagcttca gtacctgctt cagtgcatga
     1681 aagaagcgct caggctgtgg ccggtcggtc cggggttcca ggtccgtcca ctggcggacg
     1741 aggttctcgg tggccgctac cggatcaaga agggccagcc gatcctgatc ctgaccccgc
     1801 agattcaccg cgacccgaac cactttcccg acccggagtg ctacgacccg gatcgtttcg
     1861 ctccggaatt ggaagccaca cggccggggt gggcatggat accgttcggc aacggacagc
     1921 gtgcctgcat cggccgtcac ttctcctttt tcgaatcgca actgctcctc ggcctgatcc
     1981 tgcaacgatt caggctgctg gactcctttg
//
LOCUS       DAOTIP010000410         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66669_length_2009_cov_2.761515, whole genome shotgun sequence.
ACCESSION   DAOTIP010000410 DAOTIP010000000
VERSION     DAOTIP010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66669_length_2009_cov_2.761515"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="mnmA"
                     /locus_tag="P5138_08000"
     CDS             complement(<1..1373)
                     /gene="mnmA"
                     /locus_tag="P5138_08000"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRV60558.1"
                     /translation="MDSLLAEYVADRSREADAPADAFTGAAGGAACGDLARISISIDD
                     GLVGSAVFGTEGCSATRAACACVCEMAEGETVLQVARIGADRIEEELGGLSPARRHAA
                     MLAADALHRAISAAASSGETLAEASPDRVLVAVSGGVDSAVAALREQERGAEVVAVTV
                     KLWADPDTDGTKACCSPEAVLGARGLTHDLGIPHFTLDLEGPFRERVVGEFLRGYGEG
                     RTPNPCVLCNGEVRIAAMVDLADRLGASHLITGHYARVVEDEGGALVAAGRDEAKDQS
                     YMLAALPPEVVARLRFPLADLSKQEVREIAERGGLSVARKPESQDLCFLAGQSKKDFL
                     RRHGGLNDMAGPVVDEAGNELGEYPGHHHFTVGQRRGLGVAATEPLYVLGTDASTNTV
                     RVGSRSRLSTDRVRLRNAVMHRDGGRVDRVRLRYHTEPVPARIDAPAGEHKALEVELE
                     RPFDGPAP"
     gene            1422..>2009
                     /locus_tag="P5138_08005"
     CDS             1422..>2009
                     /locus_tag="P5138_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV60559.1"
                     /translation="MSKGWTADDIPDLTGRTAVVTGGNSGIGAIECRELAGHGARVIL
                     ACRNRQKGEAAVAQIRRELGAAGGDAQIEVRNLDLASLDSVREFAEGLSAEMADGLDL
                     LVNNAGVMASPRLETEDGFELQFGTNHLGHFALTGRLFPLLKKKPGSRVVTVSSIAAR
                     MGRLNFDDLNGRDGYRRWMAYGQSKLANQVFALDLQ"
BASE COUNT          320 a          695 c          664 g          330 t
ORIGIN      
        1 ggagcgggac cgtcgaaggg ccgttccagc tcgacttcga gtgccttgtg ttcaccggcc
       61 ggagcatcga tccgggccgg caccggctcg gtgtggtacc tgagcctgac tctgtcgacc
      121 ctgcccccgt cccggtgcat gaccgcgttc cgcagtcgga cgcggtcggt tgagagccgg
      181 cttcgcgagc cgaccctgac cgtgttcgtg gaagcgtcgg taccgagcac gtagagcggc
      241 tccgtggcgg caactccgag gccccggcgc tgaccgacgg tgaagtggtg gtggcccggg
      301 tactcgccca gttcatttcc ggcctcgtca acgaccggcc ccgccatgtc gttcaaaccc
      361 ccatgccggc ggagaaagtc cttcttgctc tggccggcga gaaagcagag atcctggctc
      421 tcgggctttc tggcgaccga aagccctccc ctttcggcga tctccctgac ctcctgcttc
      481 gagaggtccg ccagcgggaa cctgagccgc gcgaccactt cgggcggcag ggccgcgagc
      541 atgtaactct ggtccttcgc ttcgtccctg ccggcagcga ccagtgctcc gccctcgtcc
      601 tctacgaccc gggcgtagtg gccggtgatc aggtgcgaag caccgagccg gtcggccagg
      661 tcgaccatcg ccgcgatgcg gacttcgccg ttgcaaagca cgcaggggtt cggggtccgt
      721 ccctcgccgt agccgcgcag gaattccccg accacccgct cccggaaggg accctcgagg
      781 tcgagggtga aatgcggaat gccgaggtcg tgggtgagcc cgcgggcacc gagcaccgcc
      841 tccggtgagc agcatgcctt ggtgccgtcc gtgtccggat cggcccagag cttgaccgtc
      901 accgccacca cttcggcgcc tcgttcctgc tcgcgcaggg ccgcgacggc gctgtccaca
      961 ccgccagaga ccgcgaccag cacccggtcc ggactcgctt ccgcgagagt ctctcccgac
     1021 gatgccgcgg ccgatatcgc ccggtggagg gcatcggctg ccagcatggc agcgtggcgg
     1081 cgggccgggc tgagaccgcc cagttcctcc tcgatccggt ccgccccgat ccgggcaacc
     1141 tggagcaccg tttcgccttc cgccatttcg catacgcagg cgcaggccgc ccgggtcgcg
     1201 gagcagccct cggtgccgaa tacggccgag ccgaccagtc cgtcgtcgat cgaaatcgag
     1261 atccgcgcca gatcgccgca ggcagcacca cccgccgcac cggtgaaggc atcggccgga
     1321 gcatcggcct cccgggagcg gtcggccacg tattcggcga gcagcgaatc catcccctga
     1381 agggtagatg ttcgattccg tgaatggata gcctccgcgg catgagcaaa ggctggacag
     1441 cagatgacat ccctgatctg acgggccgca cggccgtggt gaccggggga aacagcggca
     1501 tcggggcgat tgaatgccgg gaactcgccg gccacggcgc ccgggttatc ctcgcctgcc
     1561 gcaaccggca gaagggtgaa gcggccgtcg cgcagatccg ccgggagctc ggcgcggctg
     1621 gcggcgacgc gcagatcgag gtccgtaacc tggacctggc ctcgctcgat tcggtccggg
     1681 aatttgccga agggctttcg gcggagatgg ccgatggcct tgaccttctg gtcaacaacg
     1741 cgggcgtgat ggcatcgccc cgcctcgaga cggaagatgg gttcgaactt cagttcggca
     1801 ccaatcatct cggccatttc gccctgacag gccggctctt tcccctgctc aagaagaagc
     1861 cgggatcgcg agtggtgacg gtctcctcga tcgccgccag aatgggccgc ctcaatttcg
     1921 atgatctgaa cggacgagac ggctaccgtc gctggatggc ctacggccag tcgaaactgg
     1981 ccaatcaggt cttcgctctg gacctgcag
//
LOCUS       DAOTIP010000411         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66691_length_2008_cov_3.986175, whole genome shotgun sequence.
ACCESSION   DAOTIP010000411 DAOTIP010000000
VERSION     DAOTIP010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66691_length_2008_cov_3.986175"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..979)
                     /locus_tag="P5138_08010"
     CDS             complement(197..979)
                     /locus_tag="P5138_08010"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HRV60560.1"
                     /translation="MKGRILVMGGHEFDRLDGNEAIVEHIISLTGKEAPKMCLLPTAS
                     GDPEDQISRFRRSFGSRGCEVSDISLFRLGANPIDVSAHLMKQDAIYVGGGSLVNLVA
                     VWRPHGIAELIEECLERGVMVVGQSAGAMCWFEAGITSSSGRPEPAEGLGLLEGSLCV
                     HYHRDPDRRTRYLAEVGKGIPPGYGADDQTGLLFVDGELTEAFTAREGAGVWKVTPGE
                     DGGREEAVEAIDIRPKPKSGAPGDVVDDFRALNTWRQSASLR"
     gene            complement(999..1769)
                     /locus_tag="P5138_08015"
     CDS             complement(999..1769)
                     /locus_tag="P5138_08015"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HRV60561.1"
                     /translation="MGTRRQIVAFGGGGFSMESGNPLLDDFVVSATGLKKPRVCFLPT
                     ASGDADHYLVRFYNAFRGRGEASHISLFRRERGAESIRDHLLSQDLIYVGGGSVISML
                     GVWRAHGIDQILREAWERGIVLCGLSAGSLCWFEECVTTYQNETKVTIGLGFLPWSNQ
                     VHFQLGSRGHRAFEEKLKEGLRPGYAATDGAAFHFIDTELAQVVASRPEARAYRIEMK
                     GDMPKTTQLATRYLGSPDHVPLVEESPIPWASTPIEVA"
BASE COUNT          347 a          678 c          643 g          340 t
ORIGIN      
        1 ctggaaatac cactggctgc gctcgcgctc gctcggcgcc ggcagggcga cggtcggaac
       61 cagcgcgtgg atcggtgcca gcaggatgag gaaggcgagc agtgcccccg aagccgccaa
      121 tgcgatcgtc gccggaagat gcgctgtgtg ggttctcccg ccgtcaggca cggcgggatc
      181 gtacccggcc ttcaggtcaa cggagggaag cggactgacg ccaggtgttc agggcgcgga
      241 agtcgtcgac cacgtcgccg ggtgcgccgg acttcggttt cggccggatg tcgatcgcct
      301 cgaccgcctc ttccctgccg ccatcctcgc ccggcgtgac cttccagacc ccggccccct
      361 cccgggcggt gaaggcctcg gtcaactcgc cgtccacgaa cagcagaccg gtctggtcgt
      421 ctgccccgta cccgggtggg atgccctttc cgacctcggc caggtacctg gtacggcggt
      481 ccgggtcccg gtggtagtgg acgcagaggc tcccctcgag caggcccagg ccttcggccg
      541 gctcgggccg cccggaagac gaggtgatcc ccgcctcgaa ccagcacatc gccccggcgc
      601 tctggccgac caccatcacc ccgcgctcga ggcattcctc gatcagttcg gcaatgccat
      661 gcggacgcca gaccgcgacc aggttgacca ggctgccacc gccgacgtag atcgcgtcct
      721 gcttcatcag gtgcgcggaa acgtcgatcg ggttcgcccc cagccggaag agcgagatgt
      781 cgctgacctc acagccccgg ctgccgaagg aacggcggaa ccgggagatc tggtcttcgg
      841 ggtccccgct ggcggtcggc aacaggcaca tcttcggggc ctccttgccg gtcagggaga
      901 tgatgtgctc gacgatcgcc tcgttgccgt cgaggcggtc gaactcgtga ccgcccatga
      961 ccagtatccg gcccttcatc gatctgtccg gaccttcatc aggccacctc gatcggggtc
     1021 gaggcccagg gaatcggcga ctcttcgacc agcggcacgt ggtcgggcga gccgaggtac
     1081 cgggttgcga gctgggtcgt cttcggcatg tcccccttca tctcgatccg gtaggcccgt
     1141 gcctcgggcc gggacgcgac gacctgggcg agttcggtgt cgatgaagtg gaacgccgct
     1201 ccgtcggtcg ccgcgtatcc gggacggagc ccctccttca gcttttcctc gaaggcccgg
     1261 tggcctctcg agccgagctg gaaatggacc tgattcgacc agggcaggaa gcccagcccg
     1321 atcgtcacct tggtctcgtt ctggtaggta gtgacgcatt cctcgaacca gcagagcgac
     1381 ccggccgaaa ggccacagag cacgatgccc cgttcccagg cctcccgcag gatctggtcg
     1441 atgccgtggg cgcgccagac cccgagcatg ctgatcacgc ttccgccgcc gacgtagatc
     1501 aggtcctggg agagcaggtg gtcccgaatg ctctcggctc cccgctcgcg ccggaagaga
     1561 gaaatgtggg aagcctcgcc gcggccgcgg aaagcgttgt agaaccgcac cagatagtgg
     1621 tccgcatcac cggaagcggt cggaaggaag cagacccggg gtttcttcag gccggtggcc
     1681 gaaaccacga agtcgtcaag cagaggattg ccggattcca tcgagaagcc gccaccacca
     1741 aacgccacga tctgtctccg tgttcccatg agtgaatagt tcccgcaagc aggcaatttc
     1801 cttttggcca gcgggccaga ttccgcactg ggccgtttat cccggcgaaa ctgtttggaa
     1861 acctcgccgt ttgtcaatta acggcatcag gtgccatcga cggcgccgat tgggaacttt
     1921 tcatcgtcca aaggagaacc gcttcagatg cgtacccacc cgatgtcaaa tggaggccgg
     1981 aacctcggaa acgccggact cttcctcc
//
LOCUS       DAOTIP010000412         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66700_length_2008_cov_3.286226, whole genome shotgun sequence.
ACCESSION   DAOTIP010000412 DAOTIP010000000
VERSION     DAOTIP010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66700_length_2008_cov_3.286226"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1679)
                     /locus_tag="P5138_08020"
     CDS             complement(117..1679)
                     /locus_tag="P5138_08020"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRV60562.1"
                     /translation="MVLSVWLAFVQPASANTFCVPTYSAACPASGINEAEPDLNAALS
                     AYGADGNPDKVIIAPGTLTATDSFTATGNDPLEIIGSGREKTFLTSSASGGTYVLNLG
                     GNNRTITVKDLAVVVPTSFDVGAAVGETADHFESVDLITRNEDSFGVYNPTGGSTFKD
                     IRVFGQNGASFGGVFYQQSYCLTGGITFSDIQVSDAQYGLVWNCPDTNPVTVNRAHFT
                     GADAAIQVSDGALVSVTNSLIESGEGSPIRAFNFVNKPTTLNLNHLTVVATGDPNQPA
                     IHAWVDTVASPTQNINLTLENSIISGFTSPWHLEAPIDQSKGNVILNANYTATDSAGT
                     TNGQAFVTNSNIPFGVPHFVGFDDFHLAAGSPGIDQGDPTTSLPDTDLDGKFRPVDGN
                     ADGVAVRDLGAYEFQPTCADDPAFCPPADKTAPKISKVKFSFKRRKGGTLKLRLSEAS
                     TVRVTFTPVPKKSKPKRKKVKLTRKAKAGNLTLKIGKRKLGSGRYKLTITATDKAGNK
                     SKTVTRKVRVRV"
     gene            complement(1774..>2008)
                     /locus_tag="P5138_08025"
     CDS             complement(1774..>2008)
                     /locus_tag="P5138_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60563.1"
                     /translation="QKQVSLIDLHQALTASGMVDQGLASDGLHLGVYGGEDQPDVMRG
                     SALLTIPALHYGANRRNLIWLQVLERLDQAVGE"
BASE COUNT          346 a          621 c          639 g          402 t
ORIGIN      
        1 acctcggtta ccatgctgcc cgcccgcgcc ccggacgccg gcgcggcgca gaccagaaga
       61 agcgagacgg ctgccccggc ggcaattgcg tgcctgattg ccttcaattt ttccccctag
      121 accctgaccc gaaccttccg ggtgacggtt ttcgacttgt tgcccgcctt gtcggtggcg
      181 gtgatggtca gtttgtagcg gccggaaccc agtttcctct ttccgatctt cagcgtcaag
      241 ttgccggcct ttgccttgcg ggtcagcttc accttcttgc gcttcggctt ggacttcttc
      301 gggaccgggg tgaaggtgac cctgaccgtg gaagcctcgg aaagacgaag cttcagggtg
      361 cctcccttgc ggcgcttgaa gctgaacttc accttgctga tcttcggtgc cgtcttgtcg
      421 gcgggcgggc agaaggccgg atcatcggcg caggtcggct ggaactcgta ggcgcccaga
      481 tcgcggaccg ccacgccatc cgcattcccg tcaaccggcc ggaacttacc gtcgagatcg
      541 gtgtcaggca ggcttgtggt cggatcgccc tgatcgatcc cgggcgaccc ggccgccagg
      601 tgaaaatcgt caaatccaac gaagtgcggg accccgaagg gaatgttcga attggtgacg
      661 aaagcctggc cattggtcgt cccggccgaa tcagtggcgg tgtagttggc gttcaggatg
      721 acgttgccct tgctctgatc gatcggcgcc tccaggtgcc agggactcgt gaatccgctg
      781 atgatcgagt tctccagggt caggttgatg ttctgcgtcg gactggcgac cgtgtcgacc
      841 caggcgtgaa tcgcaggctg gttcgggtca ccagttgcga cgaccgtcag gtggttgagg
      901 ttgagggtgg ttggcttgtt tacaaagttg aaagcccgga tcggcgatcc ttcaccgctc
      961 tcgatcaggg agttggtgac cgagaccagg gcgccatcgg aaacctgaat tgctgcatcc
     1021 gccccggtga agtgggcccg gttcacggtg accgggttcg tgtccggaca gttccagacg
     1081 aggccgtatt gggcgtcgct cacctgaatg tcgctgaatg tgattccccc ggtcaggcag
     1141 tagctctgct ggtagaagac acccccgaaa gaggcgccgt tctggccgaa gacccggatg
     1201 tccttgaagg tgctgccgcc agtcgggttg tacacgccga aggagtcctc gttgcgggtg
     1261 atcaggtcca ccgactcgaa atggtcggcc gtttccccta ccgccgcccc gacgtcgaaa
     1321 ctggtcggaa caaccaccgc cagatccttc accgtgatcg tgcggttgtt gccgcccagg
     1381 ttcaggacat aggtgccgcc cgaggcagag ctggtcagga aagtcttctc ccgtccggac
     1441 ccgatgatct cgagggggtc gttgccggtc gccgtgaaac tgtccgtggc ggtcagggtt
     1501 cccggggcga tgatcacctt gtccgggttg ccgtcggcac cgtaggccga aagcgccgcg
     1561 ttcaggtccg gttcagcctc gtttatgccg ctggccgggc aggccgccga ataggtcggt
     1621 acacagaagg tgttggccga ggccggttgg acgaaagcga gccaaaccga cagaaccacc
     1681 agaagaaacg cgaacttccc caaaccccgc atccagtccc cttttgtgcc tggccgccct
     1741 cctggacggc ttcattccaa ccggcgcgga agcttactcc ccgacagcct ggtccaaccg
     1801 ttccaacacc tggagccaga tcaggttgcg gcgattcgcc ccgtaatgaa gcgcggggat
     1861 cgtcagcagc gccgagccgc gcatcacgtc gggctggtcc tcgccaccgt agaccccgag
     1921 gtggaggcca tccgaggcga gtccctgatc gaccattccg gatgcggtca gggcctggtg
     1981 gagatcaatc agcgaaacct gcttctgc
//
LOCUS       DAOTIP010000413         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66881_length_2004_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOTIP010000413 DAOTIP010000000
VERSION     DAOTIP010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66881_length_2004_cov_3.000000"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="P5138_08030"
     CDS             complement(<1..501)
                     /locus_tag="P5138_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60564.1"
                     /translation="MNSPAMKLALAVGIALLTFAPSQANAAATGSISGTIQGDPTGYI
                     CARDVWVFDSAGNRVAGINGQMTGWDWDDYKIGGLEPGEYRLSFDQYCIDRPGPTPPG
                     GPSSYVGEYFNDSPDLKGATPVKVVEGTETTGVDVFLGLGNPIEWETTDDPSNAEPEP
                     ARPEARI"
     gene            complement(663..1268)
                     /locus_tag="P5138_08035"
     CDS             complement(663..1268)
                     /locus_tag="P5138_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60565.1"
                     /translation="MSAYRISDLISDAASLVRRRSSVRAVWLKKRIDPNLREQVLVAV
                     AEAVGCRICSFAHREVILDSGGSVHALADFEGEEDKDDRTFTAVVWAQSLVENDFQGT
                     SPAVDREFRDRFNDHERYDLETVATTMKLASSCGYEADGLVARLRGKPRPDSELGNEI
                     IMASLYFPPAIVGGGLLMAKRREVKGVLRDFRSFSKRFDHR"
     gene            complement(1265..>2004)
                     /locus_tag="P5138_08040"
     CDS             complement(1265..>2004)
                     /locus_tag="P5138_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRV60566.1"
                     /translation="VLMNAIPAKVAGVERLVTERGWRELGESERIGAVYDFVRDEIRF
                     GYNNSDRLPASRVLADGYGQCNTKTNLLMALLRSVGIPCRFHGATIHKELQKGVVDGF
                     FYRLAPENIVHSWAEVQYEGREVSLEGVILDHEYLQGLRSTIAPDGGPFTGYAVGTED
                     IADPPIQWRGSDTAIQATGVNNDYGTFDDPDSFYAEHGANLSGVKTVLFRTLVRHVMN
                     RKVESIRAGAPQERGADRQANKVHSAS"
BASE COUNT          370 a          644 c          607 g          383 t
ORIGIN      
        1 gatccgggct tcgggcctgg ctggttccgg ttcggcattc gaagggtcgt cggtggtttc
       61 ccattcgatg gggttgccca gaccgaggaa cacgtcgacg ccggtcgttt cagtcccttc
      121 aaccaccttg acgggggtcg cacccttgag gtccgggctg tcattgaaat actcgccgac
      181 gtacgagctg ggaccccccg gtggcgtcgg accgggacgg tcaatgcagt actggtcgaa
      241 gctgagccga tactcacccg gttcgagccc gccgatcttg tagtcgtccc agtcccaacc
      301 agtcatctgg ccgttgatgc cggccacccg gtttcccgcg gagtcgaaca cccacacatc
      361 ccttgcgcag atgtaaccgg tggggtcacc ctggatcgtc cccgagatgg atccggtggc
      421 ggccgcgttc gcctgcgaag gcgcaaatgt caacagggcg atacccacag ccagggccag
      481 tttcattgcg ggtgaattca tttccagtct cccgtcgaac tgatcctacc ccaaaggttc
      541 gcaactccgg ccggtatgaa atggaccgaa aaacgggggg acggacagcc tcatttagcc
      601 aagtccgagg aattgatggt gcggaacgcc atagacggac ctgtggaacg ggctgtgaaa
      661 cgtcagcggt ggtcgaaacg cttcgagaag gaccggaagt cgcgaagcac gcccttcact
      721 tcccgtcgtt tggccatgag aagcccgccg ccgactattg ctggcgggaa atagagcgaa
      781 gccatgatga tctcgtttcc gagctcgctg tccggcctcg gtttgcctct cagccgagcc
      841 accaggccgt ccgcctcgta gccgcatgaa ctggcgagct tcatcgtggt cgcgaccgtc
      901 tcgaggtcgt aacgctcgtg gtcgttgaac cggtcccgaa actcgcgatc gacggcgggt
      961 gaggtcccct ggaagtcgtt ctcgaccagg gattgagccc acacgaccgc cgtgaaggtg
     1021 cggtcgtcct tgtcttcttc accctcgaag tcggccagcg catgaaccga cccgcctgaa
     1081 tccaggatca cttcccggtg cgcgaagctg catatccggc atccgacagc ctcggccacg
     1141 gcgaccagga cctgctctct caggttgggg tcgatccgct tcttcaacca gacggcccgg
     1201 accgacgaac ggcgcctgac cagcgacgca gcgtcggaga tcaggtcgga aattcgatag
     1261 gcactcacga ggcagagtga accttgtttg cctgacggtc ggcaccacgt tcctgcggcg
     1321 cgccggcgcg gatggactcc accttgcggt tcattacgtg gcggacaagg gtgcggaaca
     1381 gcacggtttt cacaccgctg aggttagccc cgtgttcggc atagaaggaa tcagggtcgt
     1441 caaatgtccc gtagtcattg ttgactccgg tcgcctggat cgcggtgtcg gagccacgcc
     1501 actggatcgg gggatcggcg atgtcctcgg tgccgacggc atatccggtg aacgggccac
     1561 cgtcaggggc aatcgtgctg cgcaggccct gaaggtattc gtgatccagg atgacgccct
     1621 cgaggctgac ctccctcccc tcgtactgca cctcggccca gctgtggacg atgttctctg
     1681 gtgccagccg gtagaagaat ccatccacaa cgcccttctg aagctctttg tggatcgtcg
     1741 caccatgaaa gcggcaagga atcccgaccg acctgagcag cgccatcagc aggttcgttt
     1801 tcgtgttgca ctgcccgtag ccgtcggcca gaacccggga tgccggcaat cggtcggagt
     1861 tgttgtagcc aaagcggatc tcgtcccgaa cgaaatcgta gacggcgccg atccgctcgc
     1921 tctcccccag ctcccgccag ccacgctccg tgaccagccg ctcgaccccc gccaccttgg
     1981 ccgggatcgc attcatcagc accg
//
LOCUS       DAOTIP010000414         2003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_66930_length_2003_cov_2.537988, whole genome shotgun sequence.
ACCESSION   DAOTIP010000414 DAOTIP010000000
VERSION     DAOTIP010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66930_length_2003_cov_2.537988"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="P5138_08045"
     CDS             complement(<1..1562)
                     /locus_tag="P5138_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60567.1"
                     /translation="MSFPGYLVSCLGTLTGLAAVILTGAVLGSRLLPGWHGPVAWTGV
                     GVLAVGAAIGPALALGTFGLLTGWSWLLLTIAIAAAIWRFQERLPIARARSLPYTEPA
                     TPGLTMVGTAIAAGAFGAFVAGVSIRLGTGMTGFDSTWYHGPFAAGIAQSGDTWSLHL
                     LAPQFLSWFYPQNSEVIHSLGMMAFGNDLLSIFLNLGWFSACLLAAWCIGRPYGAAPI
                     SLAGVALILGSTAMADQAGEARNDIVGTFFLLAALAVMVNAAAGGRRITLGPALVVTL
                     AAGLAMGTKVNFVPAALVLLAGTVVLSEPSRRIRNGLAGCGALLLGGGYWYLRNLTHS
                     GNPIPWINHIGPITLPGPSQDVGGRDAGSVWGYLADTDVIRHWFLPGFGDGFGSGWIL
                     LGILAIAGLVLCLDRNSGPARRTGAVVGLALVLAWLVAPTSASGPSGEPNGFVSGLRY
                     LAPALAVCLALLGSGIGQRGSRARWLVAGALLILAPVTIAFGDDWDAKEWLVAFASGT
                     LFWVACLIGLGV"
     gene            complement(1559..>2003)
                     /locus_tag="P5138_08050"
     CDS             complement(1559..>2003)
                     /locus_tag="P5138_08050"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRV60568.1"
                     /translation="LSRTFFGLQKPWIPTWISGANLAVTALGSYLLYKPFGVAGIVAA
                     TAIATAVSVSIQTVMLRTRLAGIEFRKFLFSGGRILIGSVVLAGVSYEIWDLLDQALG
                     RGLAGQIISLGAGLGLGGVAYVAVCQLLRVPELEQVAALVRRRSS"
BASE COUNT          398 a          670 c          695 g          240 t
ORIGIN      
        1 cagactccga gcccgatcag acaggcgacc cagaacaggg tgccggaggc gaaggcgacc
       61 agccactcct tggcatccca gtcgtcaccg aaggcgatgg tgaccggagc caggatcagc
      121 aacgcccccg caacgagcca gcgtgcccgg ctccctcgct gtccgatccc gctccccaac
      181 agggccaggc agacggccag ggccggggcc aggtagcgca gtccggaaac gaatccgttc
      241 ggctcgcctg agggcccgga ggccgaggtc ggagcgacca gccaggccag gaccagggcg
      301 aggccgacca ccgctccggt ccgtcgggcc ggacccgaat tgcggtccag gcagagtacg
      361 agtccggcga tcgccaggat gcccagaagt atccagccgg acccgaaccc gtcaccgaag
      421 ccgggcagga accagtggcg gatcacgtcg gtgtcggcca ggtaacccca gaccgatccg
      481 gcgtcgcgcc cgcccacgtc ctggctgggt ccgggcaggg tgatcgggcc gatgtggttg
      541 atccacggga tcgggttgcc ggagtgggtc aggttgcgca ggtaccagta gccgccaccc
      601 aggagcaggg caccgcagcc ggccagcccg ttccgaattc gtctggacgg ctccgacagc
      661 accacggtgc cggccagaag caccagggcg gccggaacga agttgacctt ggttcccatg
      721 gcgagaccgg ccgcaagggt gacgaccaga gccggaccga gggtgatgcg gcgcccgccg
      781 gcggcggcat tgaccatcac ggccagcgcc gcgagaagga agaaggtgcc gacgatgtca
      841 tttcgggctt cacccgcctg gtcggccatt gccgtcgatc cgaggatcag ggcaacgccg
      901 gccagcgaga tcggcgcggc gccatagggt cggccgatac accaggcggc cagcaggcag
      961 gccgagaacc agccgagatt caggaaaatc gagagcaggt cattgccgaa ggccatcatt
     1021 cccagcgagt ggatgacctc cgagttctgc gggtagaacc agctgaggaa ctgcggggcg
     1081 agcagatgaa gcgaccaggt gtccccgctc tgggctattc cggccgcgaa cggcccgtgg
     1141 taccaggtcg agtcgaagcc ggtcattccg gttccgaggc ggatcgagac cccggcgacg
     1201 aaggccccga aggcacccgc ggcgatcgcc gttccgacca tggtcagacc cggggtcgcc
     1261 ggttcggtat atgggaggga gcgggcgcgc gcgatcggca accgttcctg gaagcgccag
     1321 atggctgcag cgattgcaat ggtcaaaagc agccagctcc agccggtcag gagcccgaag
     1381 gtcccgagcg ccagggccgg gccgatcgcc gcgccgacgg cgagcacgcc gactccggtc
     1441 caggcgacgg gaccgtgcca gcccggaagc agccgcgagc cgagtacggc accagtcaga
     1501 atcacggcgg caaggccggt gagcgttccg agacagctga ccagatatcc cggaaagctc
     1561 acgacgaacg ccgccggacc agcgccgcaa cctgctcgag ttcaggaacc cgcagcagct
     1621 ggcataccgc cacgtaggct accccgccca ggcccaggcc ggctccgagg gagatgatct
     1681 ggccggccag tccacgcccg agtgcctggt cgagcagatc ccagatctca tatgagactc
     1741 cggccagcac caccgacccg atcaggatcc tgccccccga gaagaggaac ttcctgaact
     1801 cgatcccggc cagcctggtg cgaagcatca cggtctggat ggacaccgat actgcggtgg
     1861 cgatggccgt ggccgcgacg attccggcca ccccgaacgg cttgtagagg aggtaggagc
     1921 cgagcgcagt caccgccaga ttggcgccgg agatccaggt cggaatccag ggcttctgga
     1981 gaccgaagaa ggtgcggctg agg
//
LOCUS       DAOTIP010000415         2000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67079_length_2000_cov_2.530591, whole genome shotgun sequence.
ACCESSION   DAOTIP010000415 DAOTIP010000000
VERSION     DAOTIP010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67079_length_2000_cov_2.530591"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="P5138_08055"
     CDS             complement(<1..334)
                     /locus_tag="P5138_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV60569.1"
                     /translation="MSGLVDRFGRVHRDLRVSLTDKCSLRCTYCMPAEGVPWIPKQSV
                     LTPDEIERIVRVAAAEGIETVRLTGGEPLISPNLVEIVRRIAAIEGPNGPIEVAMTTN
                     GIRLPDFIP"
     gene            complement(331..762)
                     /locus_tag="P5138_08060"
     CDS             complement(331..762)
                     /locus_tag="P5138_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRV60570.1"
                     /translation="MDGQATVALARISDEDLDLNRHLEAVSAPGHGAVVTFVGQIRDH
                     DPEAAGSVELVEYSAHPDAQAILERVSGEASRPGTSIAVSHRTGPVEVGQPALIACVA
                     AAHRGLAYEVSRDLVERIKRELPIWKRQVDTEGRHNWQGLR"
     gene            complement(762..1277)
                     /locus_tag="P5138_08065"
     CDS             complement(762..1277)
                     /locus_tag="P5138_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRV60571.1"
                     /translation="MDFANNPAGISVSVVTVSDRCFSGEREDGSGPAAVSTLNAAGFA
                     TEGPTVVPDGEESVGSAVKKEIESGADVIVTLGGTGAGERDLTPEGTRPLIERELPGI
                     AEGLRATSAARVPTAVLSRGISGISQATASGHRSVIVNLPGSTGGARDGVQYLTPILP
                     HLIDQLRGGDH"
     gene            1420..>2000
                     /locus_tag="P5138_08070"
     CDS             1420..>2000
                     /locus_tag="P5138_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV60572.1"
                     /translation="MRIRRLGWAGIEVESGGETVVVDHVLGWKSIESYIGPPRTPLLE
                     PESAGRSLAAFVTHLHTDHTDAAAVSQTLAEGGSLHRPAVMEGEPLETGGVMAAELEL
                     KEAAVDQVLMDEWESVEVGPFVATAVPAVDGFGDPQISWVIEADGKRIIHAGDTLFHG
                     SWWLIQMRFGPFDAAFLPVNGAITSFPHRQPASG"
BASE COUNT          312 a          681 c          667 g          340 t
ORIGIN      
        1 cggggatgaa gtcgggcagg cggatgccgt tggtggtcat cgccacttcg atcgggccgt
       61 tcgggccctc gatggccgcg atccgccgga ctatctcgac caggttcgga ctgatcaggg
      121 gctcgccgcc ggtcagcctg accgtttcga ttccctccgc ggcggcaacc cggacgatcc
      181 gctcgatttc gtccggggtg agaaccgact gcttggggat ccagggcacg ccctcggccg
      241 gcatgcagta ggtgcagcgc agcgagcact tgtcggtgag cgagacgcgc aggtcgcggt
      301 ggacccggcc gaaccggtca accaggccgc tcatcgaagc ccctgccagt tgtggcgacc
      361 ttcggtatcg acctgtcgtt tccagatcgg cagctcccgc ttgatccgct cgaccagatc
      421 ccggctcacc tcgtaggcca gcccccggtg agcggcggca acgcaggcga tcagcgccgg
      481 ctggccgacc tcgaccggcc cggtccggtg gctcacggcg atgctcgtgc ccggccggga
      541 agcttcgccg gaaacccgct cgaggatggc ctgggcgtcg ggatgagccg agtactccac
      601 cagctcgacg ctgccggccg cttcggggtc gtggtcgcgg atctgcccga cgaaggtcac
      661 cacggcgcca tgaccgggag cggacacggc ttcgaggtgc cggttcaggt cgaggtcttc
      721 gtcgctgatc cgggccaggg ccacggtcgc ctgcccgtcc atcagtgatc cccgccccgc
      781 agttgatcga tcaggtgcgg caggatcggt gtgaggtact gaactccgtc ccgggcaccg
      841 ccggtcgaac ccggcagatt gacgatcacc gagcggtggc cggatgcggt ggcctggctg
      901 atccccgaga tgccccggga gagcaccgcg gtcggaaccc gggccgcgct cgtggcgcga
      961 aggccttcgg cgatacccgg cagttcgcgt tcgatcagcg gacgggtccc ttcgggcgtc
     1021 aggtcccgtt cacccgctcc ggtgccgccg agcgtgacga tcacgtcggc ccccgactcg
     1081 atctccttct tcaccgctga accgacgctt tcttctccgt cggggacaac ggtggggcct
     1141 tcggtcgcga agccagccgc gttcaaagtg ctgaccgccg cggggccgga gccatcctcc
     1201 ctctctccgc tgaagcagcg gtcggagacc gtcaccacac tcacggaaat cccggccgga
     1261 ttgttcgcga agtccatgaa accagcatat ggagcctgcc tcttcgccca cctcgccccc
     1321 ggtcgcttga agtacgaacg atcgtatgct atctattgga tgtcaggtcg ccgggatcga
     1381 gccggccgcc gcgatcgtca aagcgatgaa gggaagaaga tgcgaatcag aagacttggc
     1441 tgggccggga tcgaggtcga atccggcggg gagaccgtgg tggtcgacca tgtcctcggc
     1501 tggaaatcga tcgagtccta catcggcccg ccccgcaccc cgttgctgga gcccgaatcg
     1561 gctggccgtt ctctggctgc cttcgtgacg cacctgcaca cggaccacac cgatgccgcc
     1621 gccgtctcac agaccctggc cgaaggcggc tcgcttcacc gtccggcagt gatggagggt
     1681 gagccgctgg agaccggcgg tgtgatggca gccgagctgg aactgaagga agcggcagtc
     1741 gaccaggtcc tgatggatga atgggaatcg gtcgaggtcg ggcccttcgt ggccaccgcc
     1801 gtgccggcgg tcgacggttt cggcgacccc cagatctcct gggtcatcga ggccgacggc
     1861 aagcgaatca tccatgccgg cgacacgctc tttcacggtt cctggtggct gatccagatg
     1921 cgtttcggac ccttcgatgc cgccttcctc ccggtcaacg gggcgatcac ctcctttccc
     1981 caccggcagc cggccagcgg
//
LOCUS       DAOTIP010000416         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67099_length_1999_cov_3.318930, whole genome shotgun sequence.
ACCESSION   DAOTIP010000416 DAOTIP010000000
VERSION     DAOTIP010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67099_length_1999_cov_3.318930"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1047
                     /locus_tag="P5138_08075"
     CDS             334..1047
                     /locus_tag="P5138_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60573.1"
                     /translation="MKGPAVAAVAVIGALVLGLALAGSMAAAPDAQAAKLTACVKKKT
                     GAMRLVSGKKAKKKCPRGWRKVTWQKDKGQSGLQVVSADGKVVGKLVGTAGDGVTLNA
                     LNVLRNGGIYTYLGGGQLSPITSMAALPSFKTADCSGQAYLGLSGSLPPAALDFYKGI
                     LGGTFRMVFRTFSPLGLGPASAWKFQGVVEDVTVGIQLYQLQTDGTCAPGDPSFTGTL
                     FQFDSEPAPPDFKGPLRIR"
     gene            complement(1077..>1999)
                     /locus_tag="P5138_08080"
     CDS             complement(1077..>1999)
                     /locus_tag="P5138_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60574.1"
                     /translation="HVEARSVLGVPLEQGMAALDKIFTLLDTEAEIVDGPEAIDPPRL
                     EGRITFDDLSFTYEDPEVEAESGEPVRPAPAEPRWAVRDIDFEIAPGETVALVGSTGA
                     GKSTLAKLITRFHDPQEGRIMIDGRPLPDYRQRPLRSQMGIVPQEGFLFSGSIAENIA
                     FGRPDASLEEIAAAADAVGASPFIERLSDGLETEVGERGVQLSSGQRQLVAFARVILA
                     EPRILILDEATSAVDTRTEKVIERALDEVLKGRTAVVIAHRLSTIRRADRIIVLERGH
                     IAETGSHDELIESGGLYAGLYSSWTDSSAG"
BASE COUNT          327 a          640 c          677 g          355 t
ORIGIN      
        1 cgagacctac actccgccgg ccacgaccac cgcacccgac acgggaaccg gcggcggcgt
       61 cgccccgacc accccgagca cgggcggtgg cacctcgacc ggcggcagca gttccggcgg
      121 cacaagcagc ggcggcatcg gccccggctg attctgcctc aatccggggg atagtcgaaa
      181 aacctcaggt gcccttccgt tgggttcggc cagtgggcgc ctctgggagg aggacccgac
      241 accgcccggc cgggggcacc gttcaagcgg ctagggtact tggactaatc caactatggg
      301 gggtctgtga tgtctgtttc gggtcggttg aagatgaaag ggcctgcggt ggcggcggtt
      361 gccgttatcg gggcgttggt actcgggctg gctctggccg ggtcgatggc tgccgctccg
      421 gatgcgcagg cagcaaagct cactgcctgc gtgaaaaaga agaccggcgc gatgcgtctg
      481 gtctcgggca agaaggcgaa gaagaagtgc ccgaggggct ggcgcaaggt cacctggcaa
      541 aaggacaagg gccagagcgg cctgcaggtt gtttccgccg acggcaaggt ggtggggaag
      601 ctggtaggaa cggctggaga cggcgtaacc ctcaacgccc tcaacgttct gcgtaacggt
      661 ggcatctaca catatctcgg tggcggtcag ctctccccga tcacctcgat ggccgccctg
      721 ccctcattca agacggcaga ttgctcgggt caggcatatc tcggacttag cggttctttg
      781 cccccggccg ctcttgactt ctacaaggga attctcgggg gcactttccg gatggtcttc
      841 cggaccttct cgccgctcgg cctgggtccg gcttcagcct ggaagttcca gggggtcgtt
      901 gaggatgtca cggtcggtat tcagctctac cagcttcaga ccgacggaac ctgcgccccg
      961 ggtgatccga gcttcaccgg caccctgttc cagttcgatt cggagccggc tccgcccgac
     1021 ttcaagggtc cgctccgaat caggtagacg gcaaggatct cgtcggccgg ctgctctcag
     1081 ccggccgacg aatccgtcca ggagctgtaa agcccggcgt agagacctcc ggattcgatc
     1141 aactcatcgt ggctcccggt ctcggcaatg tggccgcgct cgagaacgat gatcctgtct
     1201 gcccggcgga tcgtcgacag ccggtgagcg atcacgacgg cggtgcggcc cttaagtacc
     1261 tcgtcgaggg cgcgttcgat gaccttctcg gtcctggtgt caaccgccga agtcgcttcg
     1321 tcgaggatca ggatccgggg ctcggccagg atcacccggg cgaaggcgac gagctggcgc
     1381 tgtccggagg agagctggac accgcgctcg ccgacctcgg tctccagccc atcgctgagt
     1441 cgctcgatga acggtgacgc gccgactgca tcggccgcgg cggcgatctc ctcaagggag
     1501 gcgtcgggac gcccgaaggc gatgttctcc gcgatcgagc cggagaaaag gaatccctcc
     1561 tgggggacga tgcccatctg gctcctgagc ggcctctgcc ggtaatccgg aagcggtcgg
     1621 ccgtcgatca tgatgcgacc ctcctgcggg tcgtggaagc gggtgatcag cttggcgagg
     1681 gtggacttcc cggctcccgt gcttccgacc agggccacgg tctctcccgg cgcgatttcg
     1741 aagtcgatgt ccctgaccgc ccagcggggc tctgccgggg cgggcctcac cggctcaccc
     1801 gattccgctt cgacctcggg gtcctcgtag gtgaaggaaa gatcgtcgaa ggtgatgcgg
     1861 ccctcgaggc gaggcgggtc gatcgcttcc ggcccgtcga cgatctccgc ttcggtgtcg
     1921 agcagggtga agatcttgtc cagcgccgcc atgccctgct ccagcggcac cccgagcacg
     1981 gaccgggctt cgacgtgtg
//
LOCUS       DAOTIP010000417         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67182_length_1997_cov_3.636972, whole genome shotgun sequence.
ACCESSION   DAOTIP010000417 DAOTIP010000000
VERSION     DAOTIP010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67182_length_1997_cov_3.636972"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1289
                     /gene="cax"
                     /locus_tag="P5138_08085"
     CDS             156..1289
                     /gene="cax"
                     /locus_tag="P5138_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HRV60575.1"
                     /translation="MSGQAGETGKRRSMPEWFIWPYWLVPFIPVAIVLDFFVDASATL
                     IFVTAALGVIPTAALMGVATEELASKSGPGLGGLLNVTFGNAPELIIALFALGAGLQE
                     VVKASIVGAVLGNILLIMGIGMFLGGWKREKQTFNQTAVSSQALMLFLAVATLVLPAV
                     FVLVHGDGLPGVADEITDFGHDVNAISIVASGILLLTYGAGLLFSLKTHKNLFNPAGE
                     EHGSPLGWGVGKAVGMLALAGVAVGVMAHILVGSLEEVSVSLGLSEFFVGIIIVAIVG
                     NAAESWVSILYATKNKMDLSVQIAIGSATQLALVVMPTLVIVSFFLGPNPMALVFNGY
                     EMASLVIAVMAAYYVVQEGESNWFEGLQLLALYAVLGVVFFFA"
     gene            complement(1298..1651)
                     /locus_tag="P5138_08090"
     CDS             complement(1298..1651)
                     /locus_tag="P5138_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRV60576.1"
                     /translation="MAGHRIYGMSFGKVYPAYVNKAEKKGRSKEEVDEIIRWLTGYSQ
                     KQLEAVIGDGTDLETFFAEAPAMNPSRRSITGVICGVRVEEIDEPLMQEIRYLDKLID
                     ELARGKSMQKILREQ"
     gene            1721..>1997
                     /locus_tag="P5138_08095"
     CDS             1721..>1997
                     /locus_tag="P5138_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60577.1"
                     /translation="MWGKFRLALVFVACLAVIGGCSGSSGGGSGDGGSDVADLPPIQH
                     NRGPAAYRQGLAITLKLLNRFWTDKAAGKGRISDPPKALVSYWTRKQD"
BASE COUNT          326 a          628 c          610 g          433 t
ORIGIN      
        1 gcccgaaatg acacgcaacc gaatcgtcgt aaaccgttct acagagccaa aacacgcact
       61 agccagaaac gctcgggagc gatcataatc cgcgtgtcgg gggttcgagt ccctcccccg
      121 gtacttactt ccgctgtgcg tgagacgatt ccggcgtgtc cggacaggct ggagagacgg
      181 ggaagcgcag gtcgatgccg gagtggttca tctggcccta ctggctggtc cctttcattc
      241 cggttgcgat cgtcctcgat ttcttcgttg acgcgagcgc gaccctgatc ttcgttaccg
      301 cggcgctggg tgtgatcccg accgcggcgc tgatgggtgt cgccaccgag gagctcgcct
      361 cgaagtccgg ccccggactc ggcgggctgc ttaacgtgac cttcggcaat gccccggagc
      421 tgatcatcgc cctcttcgct ctcggggccg gccttcagga agtggtgaag gcatcgatcg
      481 tgggtgccgt cctcggcaac atcctcctga tcatggggat tggcatgttc ctcgggggct
      541 ggaagcggga gaaacaaacc ttcaaccaga ccgcggtctc ctcccaggcc ttgatgctct
      601 ttctcgccgt ggccaccctc gtcctgccgg cggtcttcgt gctcgtccac ggtgacggac
      661 tgcccggggt cgccgatgaa atcaccgact tcggtcatga cgtaaacgcg atctccatcg
      721 tcgcttcggg aatcctgttg ctcacctacg gggccgggct gctcttctcg ctgaaaaccc
      781 acaagaacct cttcaacccg gccggcgagg agcatggttc cccgctcgga tggggagtcg
      841 gcaaggcggt cggcatgctt gccctggccg gcgtggccgt cggcgtgatg gcccacatcc
      901 tggtcggctc gctcgaggaa gtctcggtgt cgctcggcct ctcagaattc ttcgtcggca
      961 tcatcatcgt cgcgatcgtc ggcaacgccg ctgaaagctg ggtctcgatc ctctatgcga
     1021 cgaagaacaa gatggatctt tcggtccaga tcgcgatcgg ctcggcgacc cagctggcgc
     1081 tggtcgtgat gccgaccctg gtcatcgttt ccttcttcct cggtccgaac ccgatggcgc
     1141 tggtcttcaa cggttacgag atggccagcc tggtcatcgc ggtcatggct gcctactacg
     1201 tcgtgcagga aggcgaatcg aactggttcg aaggacttca gctactcgcc ctgtatgccg
     1261 tcctcggggt cgtcttcttc tttgcctgag ccccggtcta ctgctcccgg aggatcttct
     1321 gcattgactt gcctcgggcc agctcgtcga tgagcttgtc gagatagcgg atctcctgca
     1381 tcaggggttc gtctatctcc tctaccctga ccccgcagat aacgccggtg atcgagcgcc
     1441 gggacgggtt catggccggt gcttcggcga agaacgtctc aagatcggtg ccgtcaccga
     1501 ttacggcttc cagctgcttc tggctgtaac cggtcagcca gcgaatgatc tcgtcgacct
     1561 cctccttgct gcggcccttc ttctctgcct tgttgacgta ggccgggtag accttgccga
     1621 agctcattcc atagatgcgg tgaccggcca tggacaggcg agtctaaccc cgaaaaagga
     1681 attcttccca tccttgcgcc ggtgtgagag gatcgcctcg atgtggggga aattcagact
     1741 tgctttggtc ttcgtcgcgt gtctggccgt gatcggtggc tgcagcggga gcagtggcgg
     1801 tggttccggc gacggtggtt ccgatgtggc ggatctcccc cccattcagc acaaccgggg
     1861 tcccgcggcc taccgccagg ggcttgcaat cacgctgaag ctgctcaaca ggttctggac
     1921 cgacaaggcg gccggcaagg gcaggatctc cgacccgccg aaggcgctcg tctcctactg
     1981 gacgaggaag caggaca
//
LOCUS       DAOTIP010000418         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67379_length_1993_cov_3.314757, whole genome shotgun sequence.
ACCESSION   DAOTIP010000418 DAOTIP010000000
VERSION     DAOTIP010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67379_length_1993_cov_3.314757"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="P5138_08100"
     CDS             complement(<1..835)
                     /locus_tag="P5138_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRV60578.1"
                     /translation="MTRADSGVLSRIRNFSISPERYQAVTLVALVALVTIVFTGAGVR
                     LTASGLGCPDWPKCYDQYVAPAQINAWIEYGNRLITGVVSITVIAAALLAFFRKPYRW
                     HLAFFGALLPLGVIGQAILGAYVVYKHLPPELVIFHFILSMILIDAAFALFWCSRYEP
                     GERRYSTDWVSTWAVRALIPIGQLTIFLGTITTASGPHPGDHDEELVRRFDFKGHDTL
                     EWIVQRHAAMAVIFALSVLAVIFIIRRQGGDPRAQKPLLVTFGLICLQLAIGLTQWLL
                     HL"
     gene            complement(822..1538)
                     /locus_tag="P5138_08105"
     CDS             complement(822..1538)
                     /locus_tag="P5138_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRV60579.1"
                     /translation="MMKNKFSTKLPVLLASVAVLGAVVAGCGTTSSNSDPAKLGFQDR
                     GRQLFNANCGTCHVLAQAASTGTQGPNLDDAFAAARSAGMDSDTIKGIVRAQVHRPQD
                     VNGDYPGVTMPANILEGNDLEDVAAYVAAVAGVPGIEPPTAPGEGPGAQVFANNGCGS
                     CHTLAAAESAGTLGPNLDEVIPTLSKAEIHESIVDPGAKIAAGYPNAMPSDYGTKISA
                     QQLNQLIDFLMTSAAADDKG"
     gene            1699..>1993
                     /locus_tag="P5138_08110"
     CDS             1699..>1993
                     /locus_tag="P5138_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV60580.1"
                     /translation="MSEVVLRVGPDHTLREAARKMADRSVGAAIVEHEEWPGPQILTE
                     RDVLRAVGEGLDPDIEKVCDHMSEKVITASPEWSLERAATEMSKRGIRHLTVYR"
BASE COUNT          374 a          585 c          711 g          323 t
ORIGIN      
        1 ggaggtgaag cagccactgg gtgagcccga tcgcgagctg gaggcagatc aacccgaagg
       61 tgacgagcag gggcttctgt gccctcgggt cgcctccctg tcgtctgatg atgaagatca
      121 ccgccagcac cgacagcgca aagatcaccg ccatcgcagc atgacgctga acgatccatt
      181 cgagggtgtc gtgacccttg aagtcgaacc tcctgaccag ctcctcgtcg tggtcgcccg
      241 ggtggggtcc cgaagccgtg gtgatcgtcc cgaggaagat cgtgagctgg ccgatcggaa
      301 tcagcgcccg caccgcccag gtgctgaccc agtcggtcga gtagcgccgc tcgccgggct
      361 cgtagcggga acaccagaag agggcgaagg ccgcatcgat gaggatcatc gagaggatga
      421 agtggaagat caccagctcg ggaggcaggt gcttgtagac gacgtaggcg ccgaggatcg
      481 cctggccgat cacgccgagt ggcaacaggg cgccgaagaa ggcgaggtgc cagcggtatg
      541 gcttgcggaa gaaagccagc agcgcggcgg cgatcacggt gatcgacacg acgccggtta
      601 tcagccggtt cccgtattcg atccaggcgt tgatctgggc gggggcaacg tactggtcgt
      661 agcacttggg ccagtccggg cagccgagac cggaggcggt gagtcgcacg ccggcgccgg
      721 tgaagacgat cgtgaccagc gcgaccagcg cgacaagcgt gaccgcctgg taacgctcag
      781 gcgagatgga gaaattgcga atccggctca ggacgccgga atcagccctt gtcatcagcc
      841 gcagctgatg tcatgaggaa atcgatcaac tggttcagct gctgagcgct gatctttgtt
      901 ccgtagtcgg aaggcatcgc gttcgggtaa ccggcggcga tcttcgcgcc cggatcgacg
      961 atcgattcgt ggatctcggc cttgctcagg gtcgggatga cctcgtcgag gttggggccg
     1021 agcgtgccgg cggactcggc ggcggccagc gtgtggcagc ttccgcagcc gttgttggcg
     1081 aagacctggg cgccgggtcc ttcgcccgga gcggtgggcg gctcgatgcc gggcactccg
     1141 gcgacggcgg cgacatacgc cgcgacgtct tcgaggtcat tgccttcgag gatgttggcc
     1201 ggcatggtca cgcccgggta gtccccgttc acgtcctgcg gccggtgaac ctgggcgcga
     1261 acgatgccct tgatggtgtc ggagtccatc ccggcggaac gggcggcggc gaaggcgtca
     1321 tccagattcg ggccctgggt gccggtgctg gcggcctggg cgagaacgtg gcaggtaccg
     1381 cagttcgcgt tgaagagctg ccgaccgcga tcctggaatc cgagcttcgc cgggtccgag
     1441 ttcgaagagg tcgtgccaca acccgcgacg accgcaccga ggacggcaac ggaggcgagg
     1501 aggacgggaa gcttggtcga gaacttgttc ttcatcatgg cgttcggtga agcccggcca
     1561 ttctatatat cggggcggca gccgcgtccc gcggtagtga tccggatatg gacggtttga
     1621 gacatcccgc acaatccgct acgctggcaa cgatcgttac aaactccgga aagggatcgg
     1681 atgcgcgtaa gtgtcggaat gtcggaagtt gtcctccgcg tgggaccgga tcacaccctg
     1741 cgcgaggcgg cacggaagat ggccgaccgg tcggtcgggg cagccatcgt cgagcacgaa
     1801 gagtggcccg gcccgcagat cctgaccgag cgcgacgtcc tcagggcagt cggagaggga
     1861 ctcgatcccg acatcgagaa ggtttgcgat cacatgagcg agaaggtgat cacggcgtcc
     1921 cccgagtgga gcctcgagcg ggccgccacc gagatgtcaa agcgcggcat ccgacacctg
     1981 acggtctacc gtg
//
LOCUS       DAOTIP010000419         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67652_length_1987_cov_2.817805, whole genome shotgun sequence.
ACCESSION   DAOTIP010000419 DAOTIP010000000
VERSION     DAOTIP010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67652_length_1987_cov_2.817805"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="P5138_08115"
     CDS             complement(<1..628)
                     /locus_tag="P5138_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60581.1"
                     /translation="MNKLRIIASLTALAVTSFIALPGLAAAADETITLQNSVVPRGGK
                     LYKEKRVPVNTSLSVQVHTPGTSAKVNPLKRSVMKFPTDLTYNPNNRRTPVCPDSKLN
                     QRSNLAAGVAAIVAICPRSVIGTGVAEIYIAKIHQPTALIGDPQLVIFNAGRDKHGNA
                     RMKIYAYSKTTNYGILMFGSLTKKGIENVFIPVLSNDSATASFRLDIPG"
     gene            complement(798..1262)
                     /locus_tag="P5138_08120"
     CDS             complement(798..1262)
                     /locus_tag="P5138_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60582.1"
                     /translation="MIGTGTAEIYLAKVNQPSALVGDPQMVIFNAGKDRRGNAKIKIY
                     AYSRTTNVGILMRGKLTRKGIINVAIPVLSNDSGTASFVLAIPGPPIKDGGKTIRGRD
                     ARYARIRCSKGKWVTRGTFTHGERAYPSGTPTGPSTKVNARPYKTRCHGARR"
     gene            complement(1259..1633)
                     /locus_tag="P5138_08125"
     CDS             complement(1259..1633)
                     /locus_tag="P5138_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60583.1"
                     /translation="MFNLKRFAVLACLPCAPALVGVASPGTVSAANETITLKTVVVPS
                     KGKMFKRRSVPAKMQLSVEVNTPSSSAKVNPLKRAVISFPRDLSFNPNKRRTPVCSDK
                     VLSEQSNLSAGVGRPSMSAPVP"
BASE COUNT          330 a          613 c          608 g          436 t
ORIGIN      
        1 aaccggggat gtccagccgg aacgaggccg tcgcggagtc gttgctgagg accgggatga
       61 acacgttctc gatccccttc ttggtcaatg atccgaacat cagtattccg tagttggtcg
      121 tcttggaata ggcgtagatc ttcatcctgg cgttgccgtg tttgtcccgg ccggcgttga
      181 aaatcaccag ctgggggtca ccgatcaggg cggtcggctg gtggatcttg gcgatgtaga
      241 tctcggcgac accggtacca atcaccgatc gcgggcaaat cgccacgatc gccgctactc
      301 cggccgcgag gttggatcgt tgattcaact tgctgtccgg gcagaccggt gtgcgcctgt
      361 tgttcgggtt gtaggtcagg tcggtgggga acttcatcac cgatctcttg agcggattca
      421 ccttcgccga ggtacccgga gtatggacct ggaccgagag ggacgtgttc accggaaccc
      481 gcttttcctt gtagagcttt ccgccgcgag gcaccacaga gttctggagg gtgatcgtct
      541 cgtcggccgc cgcggcaagg ccgggcagag cgatgaatga cgtcaccgca agcgctgtca
      601 ggctggcaat aattctcagc ttgttcattg gatctccttt cgtccgtggc cgtccttgcg
      661 acggcacctc tctctaaggc ctacccactc ttttcccact cacaccttat gtccaggtaa
      721 agacggaggg cccgcaccgt ctgacagtac ggaccctccg tttcatgcgc gggtttgcgc
      781 ccgtcaagtg ttttccgtca gcgccgggct ccatggcacc gggtcttgta gggcctggcg
      841 ttcaccttcg tcgaggggcc ggtcggggtg ccggacgggt aggcccgctc accgtgggtg
      901 aaggtcccgc gggttaccca tttgcccttc gagcatctga tccgggcata ccttgcatca
      961 cgtccccgaa tggtcttgcc accgtccttg atcgggggcc cgggaatcgc cagtacgaag
     1021 ctggcggtgc cagagtcgtt gctgaggacc ggaatcgcca cgttgatgat ccccttgcgg
     1081 gttaacttgc cgcgcatcag gatgccgacg ttggtggtcc tggaataggc gtagatcttg
     1141 atcttcgcgt tgccccgccg gtccttgccg gcgttgaaga tcaccatctg cggatctccc
     1201 accagcgcgc tcggctggtt gaccttggcc agatagatct ccgcggttcc tgtgccgatc
     1261 acggaacggg ggcagacatt gacggtcgcc cgactccggc cgacaggttc gactgctcac
     1321 tcagcacctt gtcggagcag accggggtgc gccgcttgtt cgggttgaag ctcagatccc
     1381 tcgggaagct gatcacggcc cgcttcagcg gattgacctt tgctgacgag ctcggcgtat
     1441 tcacctcgac cgagagctgc atcttcgcgg gaacgctgcg gcgtttgaac atcttgccct
     1501 ttgagggcac gacgacggtc ttcagggtga tggtctcatt ggcggccgag acagttcccg
     1561 ggcttgccac tccaaccagg gccggcgcgc agggcaggca ggccagaacg gcgaatcgct
     1621 tcaggttgaa cacagaatct cctctcgcgg agacgcccct aaggcgcctc catggacatc
     1681 cttgtttacc caacccggat tttcctaaca ctttctttac gaactgagtt gttcgcggta
     1741 accaggaacg ttgctttccg ctttcccctg cgggatgccg aggttgcccg gaccttcacc
     1801 gtcctcgacc cctgaggcgg aactgaaccg accagccggg tgccggacaa agctccccgg
     1861 accttcagct tcactccccg ggccgtggcg aatccggagt tcctaatctt cacccgaaag
     1921 gtggttttgc ggttcggtcg tacccggtcg ggtcccgaaa ccctggcgat ggcgatccgg
     1981 ggagccc
//
LOCUS       DAOTIP010000420         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67777_length_1984_cov_3.244168, whole genome shotgun sequence.
ACCESSION   DAOTIP010000420 DAOTIP010000000
VERSION     DAOTIP010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67777_length_1984_cov_3.244168"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="P5138_08130"
     CDS             <1..314
                     /locus_tag="P5138_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRV60584.1"
                     /translation="TLAADMEDQIPHPVKVERMERLVEAVQRRARERSQRFVGRNMEV
                     LVEGPSRNDPTRIRGRIRHNKAVNFEGTAEPGQMVEVDISAATSQTLTGTERLLSGIG
                     A"
     gene            322..1275
                     /locus_tag="P5138_08135"
     CDS             322..1275
                     /locus_tag="P5138_08135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRV60585.1"
                     /translation="MDTGAHPAEPAVGLPVIAIFGPTAIGKTGVATQLAGLMRDRGDD
                     PVAINCDSIQVYRGLEVISGAATPEDRDKLEHRLLSFVPVTEEFSAGKFGEAARAEID
                     TALDQGRQPILVGGTGLYLRAALADLEMKPVVDPRLREEVESELERRGPEALYEELPA
                     ELTGQVHPNDRKRIARLTELVRSGIDPHPDHEGGGELWTSSLRHPTLLVGLVEEDEDL
                     VPKIRARVEKMAGDGAAEEADRAVAEGASRTVRAAIGFDEFSSGDLDRIVILHRRFGR
                     RQMTWMRRMEGVELIDRTGMGDREVAAKILELAEQVPCRVA"
     gene            1272..>1984
                     /gene="dapF"
                     /locus_tag="P5138_08140"
     CDS             1272..>1984
                     /gene="dapF"
                     /locus_tag="P5138_08140"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRV60586.1"
                     /translation="MNFEKWQALGNDYIIIEADDLPWEPTQPRIQRICDPHFGVGSDG
                     VLLISRSDDPAYVAELRIFNPDGSEAELSGNGAREAILYLRKHGWTDAETFSILTASG
                     PVTPTITSERTCSVEMGRASTTSKDYPDGAPDGRGTLSSGDRDWDFQHVSIGNPQCAI
                     EAGADLEELDLGGIGPGIENNVVFPNRSNVSFYRVSGSRVRARIFERGVGETLSSGTG
                     ASGAAVAALGQGPGLLNSVL"
BASE COUNT          366 a          639 c          689 g          290 t
ORIGIN      
        1 gcaccctggc ggcggatatg gaggaccaga tcccgcatcc ggtcaaggtg gagcggatgg
       61 agagactggt cgaggcggtc cagcgacggg cccgggagcg ttcccagcgc ttcgtcggga
      121 ggaacatgga ggtcctggtc gaggggccca gccgcaacga tccgacccgg atcaggggcc
      181 gcatccgcca caacaaggcg gtcaacttcg aaggcacggc cgaacccggc cagatggtgg
      241 aagtcgatat ctcggctgcc acctcacaga cgctgaccgg caccgaacgg ctgctcagcg
      301 gcatcggcgc ctgaagaccc gatggatacc ggagctcatc cggctgaacc ggcggtcgga
      361 ctccccgtga tcgcgatctt cgggccgacc gcgatcggca agaccggggt ggcgacccag
      421 ctggccggcc tgatgcggga ccgcggcgac gacccggtgg caatcaactg cgactcgatc
      481 caggtctacc gcggcctgga ggtgatcagc ggtgcggcca ccccggagga ccgggacaag
      541 ctggagcacc ggctgctctc tttcgtaccg gtgaccgagg agttcagtgc cggaaagttc
      601 ggggaggcgg cgcgagcgga gatcgatacg gccctcgacc agggccggca accgattctg
      661 gtcgggggaa ccggtctcta cctgagggcg gctctcgcgg atctcgaaat gaagccggtc
      721 gttgatccac ggctgcggga ggaagtggag tcggaactcg aaaggagggg gcctgaggcg
      781 ctctacgaag agctgccggc cgaactgacc gggcaggttc accccaacga ccgcaagcgg
      841 atcgcccggc tgaccgaact ggtcaggtcc ggaatcgatc ctcatcccga ccacgagggc
      901 gggggagagc tctggacctc ctcgctccgc caccccaccc tgctggtggg cctcgtcgag
      961 gaagacgagg atctggtgcc gaagataagg gcgagggtgg agaagatggc aggagacggg
     1021 gccgcggagg aggctgaccg ggccgtcgcg gaaggggcgt cccggacggt gcgcgcggcg
     1081 atcggcttcg acgagttcag ctccggcgat ctcgaccgga tcgtcatcct gcaccggcgg
     1141 ttcgggcggc gccagatgac ctggatgcga cgcatggaag gcgtcgagct gattgaccgg
     1201 acggggatgg gcgaccgcga agtcgcggca aagatcctcg aactcgccga acaggtacct
     1261 tgccgggtcg catgaacttc gagaaatggc aagccctggg caacgactac atcatcatcg
     1321 aagccgatga tcttccctgg gaacccaccc agccgcgcat ccagagaatc tgcgaccccc
     1381 acttcggagt cgggtccgac ggcgtccttc tgatctccag gtccgacgac cccgcctacg
     1441 tggcggagct gcggatcttc aacccggacg gctccgaggc ggaactgtca ggcaacggcg
     1501 cccgggaagc gattctctac ctgcgcaaac acggctggac cgacgcggag accttcagca
     1561 tccttaccgc ttccggcccc gtcacgccga ctatcacctc ggagcgcacc tgctcggtcg
     1621 agatgggcag ggcttccacc acttcgaagg attatccgga cggagctccc gacggccggg
     1681 gcacactgag ctccggcgat cgcgactggg acttccagca tgtctcgatc ggaaacccgc
     1741 agtgcgcgat cgaagccgga gctgatctcg aggagctcga cctcggcgga atcggccccg
     1801 ggatcgagaa taacgtggtc ttccccaacc gcagcaacgt ctccttctac cgcgtttccg
     1861 gctcccgggt cagggcccgg atcttcgagc gcggggtggg cgagaccctt tcctccggca
     1921 ccggggcgtc gggagcggcc gtggccgcct tgggccaggg gccgggactg ctcaactcgg
     1981 ttct
//
LOCUS       DAOTIP010000421         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_67821_length_1983_cov_3.435685, whole genome shotgun sequence.
ACCESSION   DAOTIP010000421 DAOTIP010000000
VERSION     DAOTIP010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67821_length_1983_cov_3.435685"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..502)
                     /locus_tag="P5138_08145"
     CDS             complement(164..502)
                     /locus_tag="P5138_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HRV60587.1"
                     /translation="MATIGTFKKAGSEYTGEIVTLSVQAKGVRIVREDSVPNENAPSH
                     RVFVGRAEIGAAWSKRSAEGRDYLSLKLDDPSFAHPIYANLFDDTVIEGGEETFSLIW
                     SRPNGRRNGE"
     gene            complement(955..1287)
                     /locus_tag="P5138_08150"
     CDS             complement(955..1287)
                     /locus_tag="P5138_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV60588.1"
                     /translation="MRIVRHPFVERDLIGMVDHIVDVTQGDIAAAERRLDEVDALIAS
                     IIKNPRSGVRLGGGLSGWLVRHGGAGQRLTIVFRPDVETGTVFVALVAFGGRDWVRLA
                     AARRVFAD"
     gene            complement(1287..1562)
                     /locus_tag="P5138_08155"
     CDS             complement(1287..1562)
                     /locus_tag="P5138_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60589.1"
                     /translation="MSVQKQSVSFTEAAYAFARELVEAGEYPNVSAAVSGELAKAKAE
                     RDRERSLLEAELERRLALPLDQWEPVGEAGTLTSGARAHLAAARQKP"
     gene            complement(1696..>1983)
                     /locus_tag="P5138_08160"
     CDS             complement(1696..>1983)
                     /locus_tag="P5138_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRV60590.1"
                     /translation="DAHDHWPKFLGTTGKLPGAVFNQTSHALDAAMAGQGVAIACRAF
                     VQSDLDAGRLLQVLPAGFEARADYYLARKRATSSRRTVDAVWDWCIANWSC"
BASE COUNT          331 a          631 c          681 g          340 t
ORIGIN      
        1 gcgaagtcga tcgtgtcctc gccggcctcg atgcgctcct ggttggaacg accgttggcg
       61 gcgagttagt cgaggatgga tttgggaaga aggtccatgg gtctggtcct gtgctgaggt
      121 ggaatgagac cggggcgatc gaagccgccc cggtgcgggt ggctcactcg ccgttgcggc
      181 ggccgttcgg ccgcgaccag atcaggctga atgtctcctc gccgccctcg atgacggtat
      241 cgtcaaagag gttggcgtag atcggatggg cgaagctcgg gtcgtcgagc ttcaggctca
      301 ggtagtcgcg gccctcggcc gatcgcttcg accaggcggc gccgatctcg gcgcggccga
      361 cgaagacccg gtgcgagggg gcgttctcgt tcggcacgct gtcctcgcgg acgatgcgga
      421 cgcccttcgc ctggacgctc agggtgacga tctcgccggt gtattcgctg ccggccttct
      481 tgaaggttcc gatggtggcc atgtccttat ctcctcttgg ctcagtcgag cccgcgaccg
      541 tcgcggcctc gatggcgatc gacaggaccg ggacgactcg ccgacgcagc cccttggggc
      601 ccgaagcaaa gcggaggacg gcggggacgg tctttcttgg acccgccagg gtcggccgcg
      661 aggaatggcg ggcccgcccg ccaggggaag aaagtccggc accgccgttg cggccagggc
      721 ggtcgaggcg tcagccggtc acggtcagat cagccccgga gaggcccgca acgggaggcg
      781 cggcaggggc aaggaatgga gggaacaagg tccagcagcg gacctgaagt gaggccggcc
      841 gtcacatcgc cgggtcagga tcgcacctga tgcacgtgct cagcgactgc tcctgcttcg
      901 gggccagcac aggggccacg agcggccccc ctccttgggt cggaatttcc ccgatcagtc
      961 ggcgaagacc cgtcgcgcgg ccgcaagccg gacccagtcc cggccaccga acgcgacgag
     1021 agcgacaaag accgtgccgg tctccacgtc cggccgaaag acgattgtca gccgttgacc
     1081 tgcaccgccg tgtcgcacga gccagcccga aagcccgcca ccgaggcgga caccggaccg
     1141 ggggttcttg atgatcgagg cgatcagcgc gtccacctcg tcgaggcgcc gttccgcggc
     1201 agcaatgtcg ccttgggtga cgtcgacgat gtgatcgacc atgccgatca ggtcccgttc
     1261 gacgaacggg tggcggacga tccgcatcag ggtttctgtc tggccgccgc cagatgcgcg
     1321 cgggcgccac tggtcagggt acccgcctcc ccgaccggct cccactggtc gaggggaagg
     1381 gcgagccgtc gttccagctc cgcctcgaga agcgaccgct cgcggtcgcg ctcggccttg
     1441 gcctttgcca actcccccga caccgcggcg ctcacattcg gatactcgcc ggcctcgacg
     1501 agttcgcggg cgaaggcata ggccgcttcg gtgaagctga cggactgttt ctggacggac
     1561 atgctggctt ctcttctggt gctccaacag agtaccaatg tcgtgccgcg gtagcaagtg
     1621 tggtgagcag actttcgttc gcgcaagacg atcagtcagc tctgcgggtg caaagatgac
     1681 gttttttttg ggtggtcaac aggaccagtt ggcgatgcac caatcccaca cggcgtctac
     1741 cgttcttcgt gatgatgtgg cccgcttccg agccagatag tagtcggccc gcgcctcgaa
     1801 acctgccgga aggacttgga gcaggcggcc tgcatcaaga tcagactgca cgaaggcgcg
     1861 gcaagctatg gccacaccct gtccggccat agctgcgtcc agagcatggc tggtctggtt
     1921 gaacacagcg ccgggtagct tcccggtcgt gccaaggaac tttggccaat ggtcgtgtgc
     1981 atc
//
LOCUS       DAOTIP010000422         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_68206_length_1975_cov_3.042708, whole genome shotgun sequence.
ACCESSION   DAOTIP010000422 DAOTIP010000000
VERSION     DAOTIP010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68206_length_1975_cov_3.042708"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="P5138_08165"
     CDS             complement(<1..787)
                     /locus_tag="P5138_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV60591.1"
                     /translation="MSTDRRQTKGKALPGQEALLGDLRRSRSVREAAEVTARASVEQF
                     GAIGAVVSDPDGPIAARPTRLDPLTIAELTSVPAGVGVGKPGSALAGMTIACFSHPEM
                     EGMRLSAAWAPESSARIDDLESAVNALAVWLRQYSDPTLEIGNRSFTGPGPTSADYEL
                     EKEEMDSAGFEHLIDSVPAILYTAEMGESGRWLYVSPQIEKILGYTPEEWTSDTTLWY
                     RSIHPDDREHALAFEDERLIGLDIHPPAEYRLRARDGRYVWIYE"
     gene            complement(784..>1975)
                     /locus_tag="P5138_08170"
     CDS             complement(784..>1975)
                     /locus_tag="P5138_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV60592.1"
                     /translation="GKMHPGNPSKSPPDLTRESYNLTDIPLERLRRRRSAKWTYWPAD
                     VLPAFVAEMDFPLADPVKKALVEAIELDDTGYSNPSGSGLAETFAVFARRRFAWEVDP
                     GQVTATSDVVGGLRALLELAAGPREGVIINPPVYFPFFSIIPEAGRELIEVPLDESGN
                     LDLEAVGRAFEAGARAMILCSPHNPTGTVPGRTELEQLADMAAAHDAWILADEIHAPL
                     TLPGASHTPFLDVSPAARECGICVTSASKAFNIAGLSCAVIVTAGERARKLVSRLPAG
                     ATHPGHLGVIASQAAFESGDVWLDQVLAQLDFNRQHLDELLGRYLPAARYRQPAAGYL
                     AWIDATALGLGPDPATEIFDRARVAVSSGPGFGTGGQGYFRLNIGTSPELIEAAVRSI
                     AEIT"
BASE COUNT          315 a          630 c          692 g          338 t
ORIGIN      
        1 gctcgtagat ccagacatag cggccgtccc tcgccctgag gcggtattcg gccggtggat
       61 ggatgtccag gccgatcagg cgctcgtcct cgaaggcgag ggcgtgctcg cggtcgtcgg
      121 ggtgaatcga gcggtaccag agggtggtgt ccgaagtcca ttcctcgggg gtgtacccga
      181 ggatcttctc gatctggggg ctcacgtaga gccacctgcc tgactcaccc atctccgccg
      241 tgtagagaat ggccggtacc gagtcgatca ggtgctcgaa tccggccgag tccatctcct
      301 ccttctcgag ctcgtagtcg gcactggtcg gaccgggacc ggtgaaggag cggttgccga
      361 tttcaagcgt cggatccgag tactggcgca accagaccgc cagggcgttg accgccgact
      421 cgaggtcatc gatgcgggcc gagctctcgg gggcccaagc ggccgagagt cgcatgcctt
      481 ccatttcggg atgggaaaag caggcgatcg tcatcccggc aagagccgag cccggtttgc
      541 cgaccccgac cccggctgga accgaggtca gttccgcgat ggtcagcggg tccaggcggg
      601 taggccgggc ggcgatcggg ccgtccgggt cgctgaccac cgccccgatc gcaccgaact
      661 gctcgaccga cgccctcgcg gtcacctctg ccgcctccct gaccgaacgg gaacggcgca
      721 ggtcaccgag cagggcttcc tgtccgggaa gcgctttgcc cttggtttgg cggcggtcgg
      781 tactcatgtg atctctgcga ttgaccttac ggcagcctcg atcagttctg gcgaagtgcc
      841 gatgttgagc cggaaatacc cctgaccgcc ggtgccgaat ccgggtccgc ttgaaactgc
      901 gacgcgggcc cggtcgaaaa tctccgtcgc cgggtccggg ccgagtccga gcgcggtcgc
      961 atcgatccag gccaggtacc cggcggcggg ttgccggtac ctggcggccg gcaggtacct
     1021 gcccagcagc tcgtcgagat gttggcggtt gaaatcgagc tgggccagca cctgatcgag
     1081 ccacacatca cctgattcga acgctgcctg tgaggcgatg acccccagat ggccggggtg
     1141 ggttgctccg gccggcaggc gcgaaaccag cttcctggct ctctcgccgg ccgtgacgat
     1201 cacagcgcag ctcagcccgg cgatattgaa ggccttggaa gcggaggtga cgcagatgcc
     1261 gcattcgcgg gcagccgggg aaacgtccag gaaaggggtg tggctggcgc ccggcagggt
     1321 gagcggggcg tggatctcgt cggcaaggat ccaggcgtca tgggccgccg ccatgtcggc
     1381 gagttgctcg agttccgttc gtcccggcac ggtgccggtc gggttgtgcg ggctgcagag
     1441 gatcatcgcc cgggcccctg cttcgaaggc ccggccgacg gcttcgagat cgaggtttcc
     1501 gctctcgtcc agcggtacct cgatcagctc ccgtccggcc tcgggaatga tcgagaagaa
     1561 ggggaaatag acgggcgggt tgatgatcac gccttcccgc ggtccggcgg ccagttcgag
     1621 cagcgctcgc agcccgccga ccacgtcgct ggtcgccgtg acctgcccgg ggtcgacctc
     1681 ccaggcgaac cggcgacggg caaagacggc gaaggtctcc gcgagacccg aaccggaggg
     1741 gtttgagtag ccggtgtcat cgagctcgat tgcctcgacc agagccttct tcaccggatc
     1801 ggccagcggg aagtccattt cggccacgaa ggccggcagc acatcggccg gccagtaggt
     1861 ccacttggcc gaccggcggc ggcgcaggcg ctcgagcggg atgtcggtga ggttgtagct
     1921 ttcgcgggtc aaatcaggcg ggctcttcga cgggttgccc ggatgcattt tcccc
//
LOCUS       DAOTIP010000423         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_68606_length_1967_cov_2.646444, whole genome shotgun sequence.
ACCESSION   DAOTIP010000423 DAOTIP010000000
VERSION     DAOTIP010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68606_length_1967_cov_2.646444"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5138_08175"
     CDS             <1..555
                     /locus_tag="P5138_08175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRV60593.1"
                     /translation="SQGREYKINPGDGAFYGPKIDFHVTDALGRSWQLGTCQLDFQMP
                     ERFELHYTDSEDEQERPVMIHRALLGSMERFAGILIEHYAGRFPAWLAPVQARIVPVS
                     DKHLDYARQIADDLRSQGARVSVEERSESVGKKIRAAELGRFPYMLVVGDREMEAGEV
                     SVRSHEDGELGSMAVEKFCMLLDS"
     gene            649..1431
                     /gene="infC"
                     /locus_tag="P5138_08180"
     CDS             649..1431
                     /gene="infC"
                     /locus_tag="P5138_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRV60594.1"
                     /translation="MPIPRRFDRRPPERDNTRINERIRVPKVRLIGADGEQVGIVDTD
                     RARQMAREAELDLVEVAANAKPPVCRLLDYSKYKYEQEQKQKAARKHQTQVNVREIKL
                     RPKIADNDYNTKKGHVERFLKHGDKVKVTIMFRGREQAHPDRGRDLLYRLYEDLGEEL
                     AVIESRPLQEGRNMSMLLGPSKEATRTAEADKQAKKSKGKGKNRGGGAPEVPDHPNAV
                     APDAIPDHPNAVAPDAEAPEPTEPDAADEAASPAEETAETSD"
     gene            complement(1423..>1967)
                     /locus_tag="P5138_08185"
     CDS             complement(1423..>1967)
                     /locus_tag="P5138_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRV60595.1"
                     /translation="PLETCRQFGLEPGSYLLVTLHRPALVDGPLLFEAITALNEVSTL
                     MPVLFPVHPRTRARLAGNAELAENLHLVDPAGYLEFLSLESDAGAVLTDSGGIQEETT
                     YLGIPCFTLRDNTERPVTIELGTNVLLGLDPARIAEIPEIIAGQTKSVGEGQVKPPEP
                     PPLWDGHAADRIAEQLAESV"
BASE COUNT          373 a          623 c          672 g          299 t
ORIGIN      
        1 tcacagggcc gcgagtacaa gatcaacccc ggcgacggcg ccttctacgg gccgaagatc
       61 gacttccacg tgaccgatgc gctgggccgt tcatggcagc tcggcacctg ccagctggac
      121 ttccagatgc cggagcgctt tgaacttcac tacacggact ccgaggacga gcaggaaagg
      181 ccggtgatga tccaccgcgc cctgctcgga tcaatggagc gtttcgcggg gatcctgatc
      241 gagcattacg ccggccgttt ccccgcctgg ctggcgccgg tccaggcccg gatcgtgccg
      301 gtatccgaca aacacctcga ctacgcccgc cagatcgcgg acgatctgcg ctctcagggg
      361 gcgcgggtat cggtcgagga acgctccgaa tcggtgggga agaagattcg ggccgccgag
      421 ctcggcaggt tcccctacat gctggtcgtc ggcgaccggg agatggaggc gggagaggtg
      481 tccgtccgtt cgcatgagga cggggaactc ggctcgatgg cggtcgaaaa gttctgtatg
      541 ctgcttgaca gttgattcga cgcacgaaaa agacctcgcg cccagttgcg cccgcgtgct
      601 cgcctacccg tttcttgtcc gcttgacctg tccattggtt cttagctagt gccgataccg
      661 cgcaggtttg accggcgtcc ccctgaacgg gacaacactc gtatcaacga gcgcatccgc
      721 gtgcccaagg tccgtctgat cggggccgac ggcgagcagg tcgggatcgt cgataccgat
      781 cgcgcccgcc agatggcccg cgaggccgag ctcgacctgg tcgaggttgc ggcgaatgcg
      841 aagcctcccg tctgccggct gctcgactac tcgaagtaca agtacgagca ggagcagaaa
      901 cagaaggccg cccgcaagca ccagacccag gtcaacgtcc gcgagatcaa gctgcggccg
      961 aagatcgcgg acaacgacta caacaccaag aagggccacg tcgagcgttt cctcaagcac
     1021 ggcgacaagg tcaaggtgac gatcatgttc cggggccgtg agcaggccca ccccgatcgg
     1081 ggccgcgacc tgctctaccg gctctacgag gacctcggcg aggaactggc ggtcatcgag
     1141 tcgaggcccc tgcaggaggg ccgcaacatg agcatgttgc tcggaccctc caaggaagcc
     1201 acccgcacgg ccgaagccga caagcaggcg aagaagagca agggcaaggg caagaatcgc
     1261 ggcggcggcg cccccgaggt tccggatcat ccgaacgcag tcgcaccgga cgcgattccg
     1321 gaccatccca acgcggtcgc cccggacgcc gaggctcccg agccgaccga gcccgacgcg
     1381 gctgatgaag cggcgtcccc ggccgaagaa acggccgaaa cctcagactg attcagccag
     1441 ctgttccgcg atgcggtcgg cggcgtggcc gtcccagagc ggtggcggct ccggcggctt
     1501 gacttgccct tcgccgacgg acttggtctg gccggcgatg atttccggga tctccgcgat
     1561 ccgggccggg tcgaggccga gcaggacatt ggtcccgagt tcgatcgtca ccggccgttc
     1621 ggtgttgtcg cgcagggtga agcaggggat gcccaggtag gtcgtctctt cctggatgcc
     1681 gccggagtcg gtgagaacgg cgccggcgtc cgattcgagc gaaaggaact ccagatagcc
     1741 ggcagggtcg accaggtgga ggttctcggc cagctcggcg tttccggcca ggcgggcgcg
     1801 ggtccgcgga tgaaccggga agagcaccgg catcagggtc gaaacctcgt tcagggcggt
     1861 gatcgcctcg aaaagaagcg ggccgtcaac cagggcggga cggtgaaggg tgaccagcag
     1921 gtaggagcct ggttcgaggc cgaactggcg gcaggtctcc aggggcc
//
LOCUS       DAOTIP010000424         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_68756_length_1964_cov_2.749607, whole genome shotgun sequence.
ACCESSION   DAOTIP010000424 DAOTIP010000000
VERSION     DAOTIP010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68756_length_1964_cov_2.749607"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="P5138_08190"
     CDS             <1..972
                     /locus_tag="P5138_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRV60596.1"
                     /translation="NGGKLDDETEAGIEALIHEPPAPAEEPGRIHELNGAEQDYLRSL
                     ESHFRLDLKGLRVTLDCANGATYRVAGEIFTRLGAEVDLLAVEPDGRNINEGCGSTDL
                     GLLAERVASSDAQIGFAFDGDGDRVLAVDGSGRTIDGDEIISLIAAARFAAGGLGGGV
                     AVTVMSNYGFHKAMKEAGVEVEVTSVGDRYVLEALREKGWTLGGEQSGHIISTDYAPT
                     GDGIAAALMLLAALDGRDLAGALVMEKLPQVLVNVEVADREAIAGAGSVWAEVDRLNS
                     ELEGQGRVLLRPSGTEPLVRVMAEAPSQERAQEICDSLAGLVRSELG"
     gene            1090..>1964
                     /locus_tag="P5138_08195"
     CDS             1090..>1964
                     /locus_tag="P5138_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRV60597.1"
                     /translation="MCGIIGYVGNRPAEEILVAGLQKLEYRGYDSAGLAILDPENGVS
                     KVRAVGNLDGLRGALEETDLSATREPDADGHFTGIAHTRWATHGRVTEANAHPHADES
                     GKVQIVLNGIVENYVRLKGRLQDEGVSFTSETDAEVVAHLIHRYYEGDLTEAVRKAYA
                     DLRGHYSIVAMHSDSPGVLVGARKETPLIVGVGDNENFIASAIPAFLSETRLALTIDD
                     GEIVTITADGVTVTKADGEPVERESEEVSWDEETAEKAGYDTFMMKEIHEQPEAIAET
                     ILDRLHRSDRKVILAA"
BASE COUNT          359 a          634 c          674 g          297 t
ORIGIN      
        1 aacggtggga agctcgatga cgagaccgag gccggcattg aagccctgat ccacgagccg
       61 ccggcgccgg cggaggagcc cggccggatc catgagctga acggggccga gcaggactac
      121 ctccggtccc tggagagcca cttccggctg gatctcaagg gcctcagggt cacgcttgat
      181 tgcgccaatg gtgccaccta ccgggtggcc ggcgagatct tcacccggct cggcgcggag
      241 gtcgaccttc tcgcagtgga gcccgacggc cgaaacatca acgagggatg tggctccacc
      301 gacctcgggc tccttgccga gagagtcgcc tcctccgacg cacagatcgg tttcgccttc
      361 gacggcgacg gagaccgggt tctggcggtg gacgggtcgg gaaggacgat cgacggcgac
      421 gagatcatca gcctgatcgc tgcggcgcgt ttcgcggcag gtggcctcgg cggcggagtg
      481 gcggttacgg tgatgagcaa ctacggcttc cacaaggcga tgaaggaggc cggggtggaa
      541 gtcgaggtca ccagcgtcgg tgaccggtac gtcctggagg ccctgcggga gaaaggctgg
      601 acgctgggcg gcgagcaatc cggccacatc atctccaccg actacgcccc gaccggagac
      661 ggaatagccg ccgccctgat gctgctcgca gctctcgacg ggcgggacct ggccggggct
      721 ctggtgatgg agaagcttcc ccaggtcctg gtcaatgtgg aagtggccga ccgcgaggcg
      781 atcgccggtg ccggatcggt ctgggccgag gttgaccgcc tcaactcgga gctcgagggg
      841 cagggacggg tcctgctccg gccctcaggt accgaaccgc tggtcagggt catggccgag
      901 gcgccgagcc aggaacgcgc gcaggagatc tgcgattcac tcgcaggcct ggtccgcagc
      961 gaactcggct gagcggcccg gttcggcgtc cttgagcggc ccgggccggc gtccggtggc
     1021 cgggcgatat ttcgctctgt gatgcccgtc gcatgcctgg tcgcccggcc caagtagcct
     1081 gtagcgctca tgtgcggaat catcggatac gtcggcaacc gtcctgccga ggaaattctc
     1141 gtggccgggc tgcagaagct cgagtaccgc gggtacgact ctgccgggct cgcgattctc
     1201 gatcccgaaa acggcgtcag caaggtccgg gcggtcggga atctcgacgg ccttcgcggt
     1261 gccctggaag aaaccgatct ttcggcgacc agggaaccgg acgctgacgg ccacttcacc
     1321 ggcatcgccc acacccgctg ggccacgcat ggccgggtca ccgaggcaaa tgcccacccg
     1381 catgccgacg agtcgggcaa ggtccagatc gtcctcaacg gcatcgtcga gaactacgtc
     1441 cgactgaagg gccgtctcca ggatgaagga gtcagcttca cttccgaaac cgatgccgag
     1501 gtggtcgccc acctgattca ccgctactac gagggtgacc tcacggaagc ggtacggaag
     1561 gcatacgcgg acctccgcgg ccactactcg atcgtcgcca tgcactccga ttctccggga
     1621 gtcctggtcg gcgcccgcaa ggaaaccccg ctgatcgtcg gcgtggggga taacgagaac
     1681 ttcatcgcct ccgcgatccc ggcctttctc agtgaaaccc ggctcgccct gaccatcgac
     1741 gacggggaga tcgtgacgat caccgccgac ggggtgaccg tgaccaaggc cgacggcgag
     1801 ccggtcgagc gcgaatccga agaagtcagc tgggacgagg aaacggccga gaaggccggg
     1861 tacgacacct tcatgatgaa ggagatccac gagcagccgg aggcgatcgc cgagaccatc
     1921 ctcgatcgcc tgcaccgctc cgaccgcaag gtcatcctgg cggc
//
LOCUS       DAOTIP010000425         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_69610_length_1946_cov_3.235854, whole genome shotgun sequence.
ACCESSION   DAOTIP010000425 DAOTIP010000000
VERSION     DAOTIP010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69610_length_1946_cov_3.235854"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="cydB"
                     /locus_tag="P5138_08200"
     CDS             complement(<1..955)
                     /gene="cydB"
                     /locus_tag="P5138_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRV60598.1"
                     /translation="MEDTTTLQFVWFVLISILWIGYLILEGFDFGVGMLMKLIGRGND
                     EKRAILHTIGPVWDGNEVWLLVAGGATFAAFPEWYATLFSGFYIALFAILLALIVRNV
                     GFEMWGKRESSAWRTGWEWCIAVGSLLPALLWGVAWANIITGVPIEPMMTNGKESLEY
                     TGNFFDLLSVYTLLGGVASLFIFLAHGALFLEIRTEGEVREKARDMAVRTTPIAAGLG
                     AVFMAWTLIRQDSLEILSLIAAVVAVGAFVFAVLNAKANPMKAFIGTAAGIAFYFIAQ
                     FVDLFPNAMVSSIDSAFDMSLNMASSTNYTLTVMTVVAGLLV"
     gene            complement(967..>1946)
                     /locus_tag="P5138_08205"
     CDS             complement(967..>1946)
                     /locus_tag="P5138_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRV60599.1"
                     /translation="PRMVDAWAVFTNNTALIAFQHVILGAIAVAGSFLVGISWYHLWR
                     RRRAGIDTVDAQGRVVHHASDLTGRDEIDSRVWVRSLRIGAVVSVIAFVGLTITGDFQ
                     AKLMFEQQPMKMAAADAVFETEDGVGLSLFATGDFEGNPGGTNRNLQMPNALSFIMTG
                     KPDSEVKGINNLNAEYQEKYGPGEYAPIVWIAYWSWRVMLGCGFLLALFGVLGWWLNR
                     RGKLEDSRRFLKFTMPVASLPFIAAAAGWIFTEMGRQPWVVFGLLKTADANSPTVGAG
                     QIWITLVGFTLLYGVLAVFAGKLFFKTAGKGPAEIEEGSEDKPYLGLAY"
BASE COUNT          380 a          623 c          612 g          331 t
ORIGIN      
        1 ggaccagcag ccccgcgacc acggtcatga ccgtcagcgt gtagttggtc gagctggcca
       61 tgttgaggct catgtcgaag gccgagtcga tgctggacac catcgcgttc gggaacaggt
      121 ccacgaactg ggcgatgaaa tagaaggcga tacctgcggc cgtgccgatg aaggccttca
      181 tcgggttggc cttcgcgttg agtaccgcga acacgaacgc accgaccgcg accaccgcgg
      241 cgatcaggga gaggatctcc agcgagtcct ggcggatcag ggtccaggcc atgaacaccg
      301 cgccgagccc ggcggcgatc ggggtcgttc tgaccgccat gtcacgagcc ttttcccgca
      361 cctcgccttc ggtcctgatc tccaggaaga gcgcaccatg ggcgaggaat atgaacaggg
      421 atgccactcc gcccagcagg gtgtaaaccg agagcaggtc gaagaagttg cccgtatatt
      481 cgagcgattc cttgccgttg gtcatcatcg gctcgatcgg gacgccggtg atgatgttgg
      541 cccacgccac gccccagagg agggcgggaa gcaggctgcc gacggcgatg caccattccc
      601 atccggttcg ccatgccgac gattcgcgct tcccccacat ctcgaagccg acgttcctga
      661 cgatcagggc gagcaggatc gcgaagagcg cgatgtagaa gccggagaac agggtcgcgt
      721 accactcggg gaaggcggcg aaggtcgcgc caccggcgac cagcagccag acctcgttgc
      781 cgtcccagac cgggccgatc gtgtgcagga tcgcccgctt ctcgtcgttg ccgcggccga
      841 tcagcttcat cagcatgccg acaccgaagt cgaagccttc gaggatcaga tagccgatcc
      901 agaggatcga gatcaggacg aaccagacga attggagtgt tgtcgtgtct tccatttcag
      961 atccccttag taggccagtc cgaggtaggg cttgtcttcc gagccttctt cgatttcggc
     1021 cgggcccttg cccgccgtct tgaagaagag cttgccggcg aacaccgcca gtacgccgta
     1081 gagcagggtg aacccgacca gcgtgatcca gatctgcccg gcacccactg tcggtgagtt
     1141 cgcgtcagct gtcttcagca ggccgaagac cacccagggt tgtcttccca tttcggtgaa
     1201 gatccacccg gccgccgcgg cgatgaaggg caacgacgcg accggcatcg tgaacttcag
     1261 gaagcgacgg gagtcttcga gttttcctct gcggttcagc caccatccga gcaccccgaa
     1321 cagggccagc aggaacccgc aacccagcat cacccgccag gaccagtagg cgatccagac
     1381 gatcggcgcg tattcaccgg gtccgtattt ctcctggtac tcggcattca ggttgttgat
     1441 gcccttgacc tccgagtcgg gcttgccggt catgatgaag gagagggcat tcggcatctg
     1501 gaggttacgg ttggttcctc cggggttgcc ctcgaaatcg ccggtcgcaa agagcgagag
     1561 gccaaccccg tcctcggtct cgaagacggc gtcggcggcg gccatcttca tcggctgctg
     1621 ctcgaacatg agcttcgcct ggaagtcgcc ggtgatcgtc agccccacga acgcgatcac
     1681 ggagacgacc gccccgatcc gcagcgaccg cacccagacc cggctgtcga tctcgtcacg
     1741 accggtcagg tcagacgcat ggtgcacaac ccggccctgc gcatcgaccg tgtcgatgcc
     1801 ggcccggcga cgccgccaga ggtgatacca gctgatgccg accaggaagc tgccggcgac
     1861 ggcgatcgcc ccgaggatca catgctggaa ggcgatcagc gcggtgttgt tggtgaagac
     1921 ggcccaggca tcgaccatgc gcggcc
//
LOCUS       DAOTIP010000426         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_69878_length_1941_cov_2.565217, whole genome shotgun sequence.
ACCESSION   DAOTIP010000426 DAOTIP010000000
VERSION     DAOTIP010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69878_length_1941_cov_2.565217"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="P5138_08210"
     CDS             complement(<1..641)
                     /locus_tag="P5138_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRV60600.1"
                     /translation="MASRLDLDLDLDLDVFIGPFDLLLSIVLREEISLLEVELGEIVA
                     RYVEKLEAEGELELEVATEFLVLIAALLELKSRLMLPGAEEDLTDMEPAEAVEELVSR
                     MLEYRRYRDAGRALKEHFESQRGFLYRSAPLPPELRRVEVETAEKVYEPDQLAAALGD
                     LLEPPARPDLSHLRATVSLRRRLSVVRSVLLNRPVFDFDDEFGLGPGHEHRRI"
     gene            complement(747..>1131)
                     /locus_tag="P5138_08215"
     CDS             complement(747..>1131)
                     /locus_tag="P5138_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRV60601.1"
                     /translation="RVYLLETEKETRKKLGSAVTDSGREIRRGEGKEGVANLIGIYAV
                     ATGKTEEEVELEFEGAGYGDFKKAVADAVVEYLAPVREAYPEIREDEGEIERILADGA
                     AKAREIASGTLADVREAMGVGVPRG"
     assembly_gap    1132..1141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1142..1751)
                     /gene="trpS"
                     /locus_tag="P5138_08220"
     CDS             complement(<1142..1751)
                     /gene="trpS"
                     /locus_tag="P5138_08220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRV60602.1"
                     /translation="MRIFSGIQPTGRKHLGNYIGAIRQYVEGQDRGDPAIYSIVDLHA
                     ITVAYEPELLRRNLYDTTAAILAAGVDPERCILFRQSDVHEHTELCWLLSGVCAHGDL
                     NRMTQFKEKSAQQKDLVSAGIFIYPLLQAADVLAYRADEVPVGEDQRQHIELMREIAR
                     RFNERFGDTLVVPDHRIPEIGARVMDLQEPTRKMSTSVGGDGG"
BASE COUNT          317 a          645 c          599 g          370 t
ORIGIN      
        1 tcaatccggc ggtgctcgtg ccccggtccg agaccgaact cgtcgtcgaa gtcgaagacc
       61 ggacggttga gcagcaccga acggaccacc gaaagccgtc gtctcagaga gacggtcgcc
      121 cgcagatggg agaggtcggg ccgggccggt ggctcgagca ggtctcccag cgccgcggcg
      181 agctggtccg gttcatagac cttctcggcg gtctccacct caacccgcct gagctccggc
      241 ggcagcggcg ccgaccggta taggaacccg cgctggcttt cgaagtgttc cttcagagcc
      301 cgtcccgcat cccggtagcg ccggtactcc agcatccggg aaaccagttc ctcgaccgct
      361 tcggccggtt ccatgtcggt caggtcttct tccgccccgg gcagcatcag gcgggatttc
      421 agttcgagca gggcggcgat caggaccagg aactcggtcg ccacctcgag ttcgagttcc
      481 ccctctgcct cgagcttctc gacgtacctg gcgacaatct caccgagttc gacttcgagc
      541 aggctgattt cctcccgcag cacaatgctg aggagcagat cgaacggtcc gatgaaaaca
      601 tcgagatcca ggtcgaggtc caggtccagt ctggaagcca tccggaaaag cgtagagggg
      661 cgatcggtcg ccgcaaacgc gaaaacccgc tatttgcgac cgggacgggg gcggcgtttt
      721 gccgctattt gcggcaaacg ttcgggtcaa ccacgcggaa cgcccacgcc catggcctcg
      781 cggacgtcag ccagcgttcc cgaagcgatc tctctcgcct tggcggctcc gtcggccagg
      841 atcctctcga tctcgccttc gtcctcgcgg atctcggggt acgcctcccg gaccggtgcc
      901 aggtattcga ccaccgcgtc ggcgacggcc ttcttgaagt ccccgtagcc ggccccttcg
      961 aattcgagct cgacttcttc ctccgtcttg ccggtggcca ctgcgtatat cccgatcagg
     1021 ttggcgacgc cttccttgcc ttcaccccgc cttatctccc ggcccgagtc ggtcacggcc
     1081 gagccgagct tcttgcgggt ttccttctcg gtttccagca ggtacacacg gnnnnnnnnn
     1141 nggccaccgt cgcctccgac cgaggtggac atcttgcggg tcggttcctg aagatccatg
     1201 acccgggcac cgatctcggg aatcctgtgg tccggcacga ccagcgtgtc tccgaatcgt
     1261 tcgttgaacc tgcgggcgat ctcgcgcatc agctctatgt gctggcgctg gtcctccccg
     1321 accggcacct cgtctgcccg gtaggcgagc acgtcggccg cctggagcaa cgggtagatg
     1381 aagattccgg ctgaaaccag gtctttctgc tgggcggatt tctccttgaa ctgggtcatc
     1441 cggttcaggt ctccgtgggc gcagaccccg gagagcagcc agcagagctc ggtgtgctcg
     1501 tgaacgtcgg actggcggaa gaggatgcag cgctcggggt cgactccggc cgccaggatc
     1561 gcggcggtcg tgtcgtacag gttccgacgc agcagctccg gctcgtaggc gacggtgatc
     1621 gcgtggagat ccacgatcga gtagatcgcc ggatcgcccc ggtcctggcc ttccacgtac
     1681 tgcctgatcg caccgatgta gttgccgagg tgcttgcgcc cggtcggctg gattcccgaa
     1741 aagatacgca tcgcaggcaa ccctagtggt agcgtcaatt cgaggtgtcc gaccccgccg
     1801 aaatgtccac cgggcagcta acccgggaag aacgcttgtc ctccgtctac tcgtaccgga
     1861 tcacctgccg actcgaggag gtggccgggg cgatcgtccc ggttcattcg gattttcccg
     1921 agaccgggtt cctgaaccgg a
//
LOCUS       DAOTIP010000427         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_70235_length_1934_cov_3.764236, whole genome shotgun sequence.
ACCESSION   DAOTIP010000427 DAOTIP010000000
VERSION     DAOTIP010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70235_length_1934_cov_3.764236"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..825)
                     /locus_tag="P5138_08225"
     CDS             complement(232..825)
                     /locus_tag="P5138_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60603.1"
                     /translation="MAETAQPVSEACQILAATASALPEQTRHELARLLSHSVAMPTAQ
                     ELREMRLGLLVEMVKDGTLPRTKDYDELRNARRKAGADWPGSTGLILHYGTWAATSRA
                     AVDLAFHETTNRARARTPHMWPIVPYTRKEIVEALELASEKVGQPIGQWEYVELRRVE
                     RQLAWRNGSPDPRYPELGVIRKHFGGWDAAISQIVGP"
     gene            complement(818..>1934)
                     /locus_tag="P5138_08230"
     CDS             complement(818..>1934)
                     /locus_tag="P5138_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60604.1"
                     /translation="DGPAEESLRTSDLLARALFSQLRNRTIHERRYVLEALIAASNGG
                     EMTSPRMRVMKASLAWYQAESGKIPAKVAYERWRKEQDDPGLCPANRMIRHFSTWNRA
                     LNDLGIKPMPDPPALRVLSRGQQISDEEVLQALRDCAADLGSDNFTIAQYEKWAKAEL
                     LKPENRGRKIPISKSIATRRWGTVRQAKIAAGLDPNSPYHSTGHFTDEELLDQLIGAR
                     EQIEGRLSTAKYAVWRKAKLEEARRRGEVIRVPCAFTFHSHFGGWLNAVAKIEGLPIN
                     PHEHRGPPVYTPDWLAEQLLIAYDELGEPFFTSSYVQWVRDKRKEVDVDFPPPDYTTV
                     TRHGVSWPEVRDKVRVAIKTGNIEPLIEQFKKGGMNG"
BASE COUNT          327 a          566 c          597 g          444 t
ORIGIN      
        1 ccaaggccgc cgtggtctcc tcttcaggag cgggaaccaa agcctcagcg ggatcggctg
       61 ttacatcctg tgaccgctcc acgtcatctg tcatcgatgg actctcggca caagggttat
      121 gcgacccgag gcgggtctat ttcgggacgg aagctgattc cgcatgaccc ctattttgag
      181 gtcgtggtcg gacggccgag cccgagactc gacgtcggtt tcaacccgaa tctatgggcc
      241 cacaatctgg gaaatggcgg cgtcccaccc tccgaagtgt tttcggatga cgccgagttc
      301 ggggtagcgc gggtccggtg aaccgttgcg ccaggcgagt tgtcgttcca cccgtcgaag
      361 ttcgacgtat tcccattgtc cgatgggttg cccgactttt tctgacgcga gctcgagcgc
      421 ttcgacgatt tccttgcgcg tgtagggcac gatcggccac atgtgcgggg tcctggcacg
      481 ggcgcggttg gtggtttcgt ggaatgcgag atcgaccgcg gcccttgatg tggccgccca
      541 ggttccatag tgaagaatca gtccggtgct gcccggccag tcggcgccag ctttgcgtcg
      601 agcgtttcgc aactcgtcgt agtccttggt gcggggaagg gttccgtctt tgaccatctc
      661 gaccagtagc ccgagccgca tttcccggag ttcttgtgct gtcggcatgg ccaccgagtg
      721 gctcaggagc cgcgcgagtt catggcgagt ttgctcgggt agggcggagg cggtagcggc
      781 gagaatctgg caggcctcac tgaccggttg tgctgtttca gccattcatt ccgcctttct
      841 tgaactgttc gatgaggggt tcgatgtttc cggtcttgat cgcaaccctt accttgtcgc
      901 ggacctctgg ccaggaaacg ccgtgacggg tgacggtcgt gtagtccggg ggagggaagt
      961 cgacgtcgac ttctttgcgt ttgtctctca cccattggac gtagcttgag gtgaagaacg
     1021 gttcgccgag ttcgtcgtag gcgatcagaa gctgctcggc cagccagtcc ggtgtataga
     1081 ccggcggacc gcgatgctcg tgcggattaa tcggaaggcc ttcgatcttt gctaccgcgt
     1141 ttagccagcc accgaaatgg gagtggaaag tgaacgcaca gggaactcga atgacttcac
     1201 cgcgccggcg ggcttcttca agtttggctt ttcgccagac ggcgtacttg gcggtactga
     1261 gccggccttc gatctgctcg cgagccccaa tcagttggtc aaggagttcc tcatcggtga
     1321 agtggccggt tgagtgatac ggagagttcg ggtcgaggcc agcggcaatc tttgcttggc
     1381 ggacggtacc ccagcggcgg gtcgcgatcg atttcgagat cgggatcttt ctgcccctgt
     1441 tctccggttt caggagctcc gccttcgccc acttttcgta ctgcgcgatc gtgaaattgt
     1501 ccgaaccaag atccgcggca cagtcacgaa gcgcctgaag gacctcttcg tcagagatct
     1561 gctggccccg tgacaacacc ctcagcgccg gcggatccgg catcggcttg atgcctaagt
     1621 cattcaacgc tcgattccag gtgctgaaat gacggatcat tcgattcgcc ggacacagtc
     1681 ccggatcgtc ctgttccttt cgccacctct cataggcaac cttcgccggg atttttccgc
     1741 tctcggcctg gtaccacgcg agactggctt tcatcacccg catccgcgga cttgtcatct
     1801 ccccaccgtt gctcgccgcg atcaaggctt cgaggacgta tctgcgctca tgaatcgttc
     1861 ggttgcgaag ctgcgagaac agcgccctgg ccaagaggtc agaggtacgt aacgactctt
     1921 cggcagggcc gtcc
//
LOCUS       DAOTIP010000428         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_70542_length_1928_cov_2.687133, whole genome shotgun sequence.
ACCESSION   DAOTIP010000428 DAOTIP010000000
VERSION     DAOTIP010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70542_length_1928_cov_2.687133"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="P5138_08235"
     CDS             complement(<1..1665)
                     /locus_tag="P5138_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV60605.1"
                     /translation="MESGTGQLFESEASFNGAVEVVHVIWSADDDGGFAVVEVRDDSG
                     EEFVATGTIGHLKPGERAKLTGQWSEHQKYGPQVQVFTALPMDPSDRAGQVAYLSSLR
                     HIGPVRAEALCEMYGPEVLEKIAADPEGIIGSLPGLGAKQRESALESWYETRAVRDLH
                     VELAPHGLAHLAAKIHARFGDRAMRTIREDPYSLTEIDGVGFARADTIAIAAGFPPES
                     DRRAQAAAWFLLGEAERQGHTHLPLNALVAQATKLLGYRPEAEVLTSASGVITEGERV
                     YRQSTLDRECWLAGDLKERATAESVLGHSPEQSGDEKLTAEQWQAVVGAFSSRLSVVT
                     GGPGVGKTVCTREIVREAKTAGVRVALCAPTGRAARRLSEATGAEAMTIHRMLEWRPG
                     SEPTYKPGHPLPVGLLVVDEASMINLHMADVLLNGLGHDTHIVFVGDADQLPPVGAGK
                     PFADLIESGIAPVTRLTHIFRQAARSMITTAAHEVNEGRPPHLSPGPDQVQDFHFLER
                     VTPERVRGAVVEMVGDRVQAGLGLDPIRDAQVLAPIYRGEAGIDVLN"
     gene            1709..>1928
                     /locus_tag="P5138_08240"
     CDS             1709..>1928
                     /locus_tag="P5138_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60606.1"
                     /translation="MAESAKPLGDAVAEATEAASLPKSAPRKRAGKAQDVFGGEMRRR
                     DLLPLLIFHLIRDEPSYGNQLIDAIETLT"
BASE COUNT          297 a          663 c          650 g          318 t
ORIGIN      
        1 gttgaggacg tcgattcctg cttcaccccg gtagatcggg gcgagcacct gggcgtcacg
       61 gatcggatcg agtccgagcc cggcctggac ccggtcgccc accatttcga ccacagcgcc
      121 ccggacccgc tccggggtca ccctctcgag gaaatggaag tcctggacct ggtccggtcc
      181 cggggacagg tgtggcggcc ggccttcgtt cacttcgtgg gcggcggtgg tgatcatcga
      241 gcgggcggcc tggcggaaga tgtgggtcag cctggtcacc ggggcgatcc ccgactcgat
      301 caggtcggcg aacggcttgc cggcgccgac tggtggcaac tggtcggcgt cccccacgaa
      361 gacgatgtgt gtgtcgtggc ccaatccgtt cagcaggacg tcggccatgt gcagattgat
      421 catcgaggct tcgtcgacta cgaggagccc gacaggaagc gggtgaccgg gcttgtaggt
      481 gggctccgat ccgggccgcc actccagcat ccggtggatg gtcatagcct ctgcgccggt
      541 tgcctcgctg aggcggcggg cggcccggcc ggtcggggcg cagagggcga cccgcacccc
      601 tgctgtcttg gcttcccgca cgatctctcg ggtgcagacg gtcttgccga cgccgggacc
      661 gccggtgacc accgacaggc gtgaactgaa agcgccgacc acggcctgcc actgttccgc
      721 ggtcagcttc tcgtcaccgc tctgttcggg tgaatgcccg agtaccgatt cggcggtcgc
      781 ccgctccttc aggtctccgg ccagccagca ctcgcgatcc agggtcgact gtcggtagac
      841 cctttctccc tcggttatca ccccggaggc cgaggtcaga acctccgctt ccggccggta
      901 gccgagcagt ttcgtggcct gggcgacaag tgcgttcagc ggcaggtggg tgtggccctg
      961 tcgttcggcc tcgcccagca ggaaccaggc ggcggcctgg gcgcgtcggt cggactcggg
     1021 cgggaacccg gcggcgatcg cgatcgtgtc ggcccgggcg aagccgaccc cgtcgatctc
     1081 ggtcagcgaa taggggtcct cgcggatcgt gcgcatcgcc cggtcgccga aacgggcgtg
     1141 gatcttcgcg gcgagatggg cgagaccatg gggagcgagc tcgacgtgca ggtcacggac
     1201 cgcccgggtc tcgtaccagc tctccagggc cgattcgcgc tgcttcgcac cgaggcccgg
     1261 cagcgagccg attattccct ccgggtccgc ggcgatcttc tccagaacct ccggcccgta
     1321 catctcgcag agggcctccg cccgtaccgg cccgatatgc ctcagcgagc tcaggtaggc
     1381 gacctggccg gcccggtccg aagggtccat cggcagcgcg gtgaacacct ggacctgcgg
     1441 gccatacttc tggtgctcgc tccactggcc ggtcagcttc gcccgctcgc cgggcttcag
     1501 atgcccgatc gtcccggttg cgacgaactc ctcgccggaa tcgtcacgga cctcgaccac
     1561 ggcaaagcca ccgtcgtcgt cggcggacca gatcacatga actacctcga ccgcgccgtt
     1621 gaacgaggcc tcactctcga atagctgtcc ggtgccagac tccaccaagt gagagtaatg
     1681 ggcgaggcgg agactagact caccaacgat ggctgagtcc gcgaagccgc ttggagatgc
     1741 ggtcgccgaa gcgaccgagg cagccagcct gcccaagtcc gcgccccgca agcgggccgg
     1801 caaggcacag gacgtgttcg gcggcgagat gcgccggcgg gacctgctgc cgcttctgat
     1861 cttccacctg atccgcgacg aaccctctta cggcaaccag ctgatcgacg cgatcgagac
     1921 cctgaccc
//
LOCUS       DAOTIP010000429         1926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_70635_length_1926_cov_3.801710, whole genome shotgun sequence.
ACCESSION   DAOTIP010000429 DAOTIP010000000
VERSION     DAOTIP010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70635_length_1926_cov_3.801710"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="P5138_08245"
     CDS             <1..987
                     /locus_tag="P5138_08245"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRV60607.1"
                     /translation="GNVGREMMNVLAERDFPASEVVPFASERAAGKPVTFGEQSLECV
                     VPGDETIRDIDILISSAGGSVSAEWAPKFVEAGAVVVDNTSYWRMHEDVPLVVAEVNP
                     EAAQNHKGIIANPNCTTMVMMLVLAPIHREAGLERLVLSSYQAVSGTGQKAIAELTDQ
                     AGQVLNGEEIDEPKVYPHQIAFNVIPQVETFKDGDDYTTEERKVMAETRKILGLGDEV
                     KISATCARVPVFTGHSESLNIETREDLSPDRAREILAAAENVVVVDNPAAGEYPTALD
                     AAGIDQTLVGRIRRDPGQENTLNLWLAGDNLRKGAATNAVQVAELLVRNNWL"
     gene            complement(1030..1506)
                     /locus_tag="P5138_08250"
     CDS             complement(1030..1506)
                     /locus_tag="P5138_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV60608.1"
                     /translation="MADHIVRSVIREVTPLKTTDPVGYAARLVIEAGLPALPVVDEDG
                     SYAGLFGEREFMTAVFPGYVGTLMSARMIRRSLDETIERRIGSQEEPVGGYLTTDPVL
                     VEDDYADTQLAETFLHHRVMVVPIATEGRVHAVVTRGEFFQALYERIADIAEDFGD"
     gene            complement(1545..>1926)
                     /locus_tag="P5138_08255"
     CDS             complement(1545..>1926)
                     /locus_tag="P5138_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60609.1"
                     /translation="GNCHTVADRNINGNRNAELVGIVWTASVVGGIVDNIPLTAAMIP
                     VVDQIQAAGGHSGDSAHWWALSLGACFGGNLTLISAAANVAALGLAERAGRPIGFWQF
                     LKIGFPVTIASTIMATAYILLRYG"
BASE COUNT          360 a          633 c          636 g          297 t
ORIGIN      
        1 ggcaacgtgg gccgggagat gatgaacgtg ctcgccgagc gggacttccc cgcctccgag
       61 gtggtccctt ttgcctccga acgggcggcc ggcaagccgg tgaccttcgg ggagcagagc
      121 cttgaatgcg tcgttcccgg tgacgagacg atccgggata tcgacatcct gatctcctcg
      181 gccggcggct cggtcagcgc cgaatgggcg ccgaagttcg tcgaggccgg ggcggtcgtg
      241 gtcgacaaca ccagctactg gcgcatgcat gaggacgtgc cgctggtcgt cgcagaggtg
      301 aacccggagg cggcacagaa ccacaaggga atcatcgcca acccgaactg cacgaccatg
      361 gtgatgatgc tggtcctcgc cccgatccac cgcgaagcgg ggcttgagcg actggtcctt
      421 tccagctacc aggccgtgtc gggcaccggc cagaaggcga ttgccgagct gaccgatcag
      481 gccggccagg tcctgaacgg ggaagagatc gacgagccga aggtctatcc gcatcagatc
      541 gccttcaacg tgattcccca ggtcgagacc ttcaaggacg gcgacgatta caccactgag
      601 gaacgcaagg tgatggccga gacccgcaag atcctcggcc tcggtgacga ggtgaagatc
      661 tccgccacct gcgcccgggt gccggtcttc accggccatt ccgaatcgct caacatcgag
      721 acccgtgagg acctcagccc ggaccgggcc cgggagatcc tggccgcggc cgagaacgtg
      781 gtcgtggtcg acaatccggc cgccggcgag tacccgaccg cgctcgacgc ggccgggatc
      841 gaccaaaccc tggtcggccg cattcgccgc gaccccggtc aggaaaacac gctgaatctc
      901 tggctggccg gcgacaacct ccgcaagggc gccgccacca acgcagtcca ggtagccgag
      961 ctcctggtcc gcaacaactg gctgtaaaag cgaaggcccg agagagcgga gaactccgct
     1021 ctccggggcc tagtcgccga agtcctcggc gatgtcggcg attcgttcgt agagcgcctg
     1081 gaagaactcc ccgcgggtga ccaccgcgtg gacccggccc tcggtcgcga tcggcaccac
     1141 catcacccgg tggtgaagaa atgtctcggc cagctgggtg tcggcgtaat catcctcgac
     1201 caggaccggg tcggtggtca gatacccacc gaccggttcc tcctggctgc cgatccggcg
     1261 ttcgatcgtt tcgtcgagcg agcggcggat catccgggcg ctcatcaggg tgccgacgta
     1321 tccgggaaag accgcggtca tgaattcgcg ctcgccgaaa aggcctgcgt agctgccgtc
     1381 ctcgtcaacc acgggcaaag ccgggagccc cgcctcgatc acgagccggg ccgcgtaccc
     1441 caccgggtcg gtcgtcttca gcggcgtcac ctcacggata accgatctga ctatgtggtc
     1501 tgccaaaaca ccacctcgga gaaactagcc ggtcgacggg acatctagcc gtacctcagc
     1561 aggatgtagg ccgtcgccat gatcgtggag gcgatcgtca ccggaaagcc gatcttgagg
     1621 aactgccaga aaccgatcgg ccggccggcc ctttcggcga ggcccagggc ggcgacgttc
     1681 gctgccgccg agatgagggt gaggttgccc ccgaagcagg cacccagcga aagggcccac
     1741 cagtgggccg agtcgccgga gtggccgccg gcggcctgga tctgatcgac cacggggatc
     1801 atcgccgcgg tgagcggaat gttgtcgacg atgccgccta ctaccgacgc ggtccagacg
     1861 attccgacca gctcggcgtt gcggttgccg ttgatgttgc ggtcggcaac ggtgtgacag
     1921 ttgccg
//
LOCUS       DAOTIP010000430         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_70698_length_1925_cov_3.370053, whole genome shotgun sequence.
ACCESSION   DAOTIP010000430 DAOTIP010000000
VERSION     DAOTIP010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70698_length_1925_cov_3.370053"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="P5138_08260"
     CDS             <1..364
                     /locus_tag="P5138_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60610.1"
                     /translation="LDRRQIFAYLDHTDPVSLDVTLLTVADRLAARGTASIASREMVA
                     GHMELARDMVGFALDWDRNGPPAQFLPGNELAARLGIEPGPELGKVMKELAAARFAGE
                     ITDANEAVEHARGFLEAG"
     gene            384..602
                     /gene="infA"
                     /locus_tag="P5138_08265"
     CDS             384..602
                     /gene="infA"
                     /locus_tag="P5138_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRV60611.1"
                     /translation="MAKEEKIEMEGEITEALPNTMFRVKLDNDHEVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLTRGRITYRYK"
     gene            676..1515
                     /locus_tag="P5138_08270"
     CDS             676..1515
                     /locus_tag="P5138_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60612.1"
                     /translation="MLRSPLTSDGLRAARILTFLTAVALLVTLAFASHSEAKSTKRIS
                     LGARNLATLAPNCGRDFSRDCTVEGKLTAFQSLTSRYPGRNFVVPFNGKLVTWSISLA
                     NPTRGDIDNNPAQLPAFNTIFGAPAQAGISILRQVEKRKKGGPRFKLVRKSPIEILNP
                     YFGTTVTFALENPLNVYEGNVVALTIPTWAPALWSPRVCNGTIYGDLDPGKCDQARER
                     YTWRGSRVSRGDPATCELSNDGGPNEQLQKTAPQTRIESVRRYGCYYGGNVLLYSALI
                     VGR"
     gene            1628..>1925
                     /locus_tag="P5138_08275"
     CDS             1628..>1925
                     /locus_tag="P5138_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60613.1"
                     /translation="MAEFRSHLNVSRPALRVGALIIATLGLLACLAVSGVFTEADGKS
                     NRRVLLGATKNRVTPNCGTQAGARACIAEGKVTGFQVFQKGTRGKNFIVPFGKGK"
BASE COUNT          359 a          667 c          574 g          325 t
ORIGIN      
        1 gctcgacagg cgccagatat tcgcctatct cgaccacacc gacccggttt cccttgatgt
       61 gacgttgctc acggttgccg accggctggc cgcccgggga accgcctcga tcgccagccg
      121 ggaaatggtc gccgggcaca tggaactggc ccgtgacatg gtcggtttcg cgcttgactg
      181 ggacaggaat ggcccgccag cgcagttcct gcccggaaac gagcttgctg ccaggctggg
      241 gatcgaaccg ggccccgaac tcggaaaggt gatgaaagag ctggccgcgg cacggttcgc
      301 gggtgagata actgatgcaa acgaagctgt agagcacgcc cggggcttcc tcgaagccgg
      361 gtgagtagta gcctcacctc cgcatggcta aagaagaaaa gatcgaaatg gagggcgaaa
      421 tcaccgaagc cctcccgaat acgatgttcc gggtcaagct ggacaacgac cacgaggtcc
      481 tcggccacat ctcgggcaag atgcgccgcc actacatccg catccttccc ggggaccggg
      541 tcaagatcga gctttcgccc tatgacctga cccgcggccg catcacctac cgctacaagt
      601 aacccctgtc acgaccggga cccgaccggg ccccggagcg caacattgag gcacccaacc
      661 gtgttccagt attcgatgct tcgctccccc ctcacatcag acggcctccg ggccgcccgg
      721 attctcacgt tcctgaccgc tgttgcgctc ctggtcacgc tggctttcgc gtcccattcg
      781 gaagcgaaat ccaccaagcg gatctcgctc ggcgcccgca acttggcgac ccttgccccg
      841 aactgcggca gggacttcag ccgcgactgc acggtcgaag ggaagctgac cgccttccag
      901 tcgctcactt cccgttaccc gggacgcaac ttcgtcgtgc cgttcaacgg caagctggtc
      961 acctggtcga tctccctggc caatccgacc cggggtgaca tcgacaacaa tccggcccag
     1021 ctgccggcct tcaacacgat cttcggcgcc cccgcccagg ccgggatctc gatcctgcgt
     1081 caggtcgaga agcggaagaa gggcggcccc cgcttcaagc tggttcgcaa gagcccgatc
     1141 gagatcctca atccgtactt cgggaccacg gtcaccttcg ccctggagaa tccgctcaac
     1201 gtctatgagg gcaatgtcgt agcactgacc atcccgacct gggcacccgc cctctggtct
     1261 ccgcgggtct gcaacggcac gatctacggc gacctggacc ccggcaagtg cgatcaggcg
     1321 cgcgaacgct acacctggcg cggcagccgg gtttcccgcg gcgaccccgc gacctgtgag
     1381 ctcagcaacg acggcggacc gaacgagcag cttcagaaga ccgcaccgca gacccggatc
     1441 gaatcggttc gcaggtacgg ctgctactac ggcggcaacg tgctgctgta ctcggcgctg
     1501 atcgtcggtc gctgacccgg gtatccggac cggtctcgcc ggcgagaccg gtccgggcag
     1561 gatttcccgt catctccttc cacttgaact tccgcaacga gggcgtagcc tcggtgttgc
     1621 tttctatatg gccgaatttc gttcacatct caatgtgagc cgcccggccc tccgcgtcgg
     1681 cgcgctcatc atcgccactc tgggtctgct cgcctgcctt gccgtcagcg gggtcttcac
     1741 cgaggccgac ggcaagagca accggcgcgt cctgctcgga gccaccaaga accgcgtgac
     1801 gccgaactgc gggacgcagg ccggtgcgag ggcctgcatc gcggagggca aggtcaccgg
     1861 cttccaggtc ttccagaagg gaacccgggg caagaacttc atcgtgccct tcggcaaggg
     1921 caagg
//
LOCUS       DAOTIP010000431         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_70895_length_1921_cov_3.675777, whole genome shotgun sequence.
ACCESSION   DAOTIP010000431 DAOTIP010000000
VERSION     DAOTIP010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70895_length_1921_cov_3.675777"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="P5138_08280"
     CDS             <1..479
                     /locus_tag="P5138_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRV60614.1"
                     /translation="LLATQSPVATDLRLISALLHVIKNAERMGDQCVNICKLIPIVGH
                     EPPADEKMISLILAMGTQARSQIVQAQKAFADRDEAAAQDLVRQDDLIDKLNRECFTL
                     AIELGDEPDRREWAMTMLLAARAIERIGDNAVDVGEQVAFVVTGLFREFEDASHPI"
     gene            481..846
                     /locus_tag="P5138_08285"
     CDS             481..846
                     /locus_tag="P5138_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRV60615.1"
                     /translation="MEQELEAAKSQLSDSADHVLKAGPLEVRLDQRTVLADGRGIVLT
                     VREFHLLAVLASRPEQVVSREEIYDLVWSGRMPEHDRSVDVYVSKLRHKLESALPHWN
                     FIQTHIGFGYCFSPKPLAS"
     gene            978..>1921
                     /locus_tag="P5138_08290"
     CDS             978..>1921
                     /locus_tag="P5138_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRV60616.1"
                     /translation="MNLKKWFVAVPLTLALAFGVAACGSSDSDSGGSSGDTVSGEIAG
                     AGSSAQEAAMNGWIAAFQDANPDATLSYDPVGSGGGREQFIAGGTSFGGTDSPMDETE
                     LADVAKRCDPGDYIEIPVYISPIAMAYNIDGVDDLNLSADTAAQIFAGDITKWNDPPI
                     AADNPNADLPDQDIVVVHRSDESGTTENFTDYLHQNSPKYWPDEGDGVWPLKGGEAAN
                     GTSGVVSAIKSGSGTIGYADLSQVGDLNVANVKVGDSYVAPSAEAAAKIFEESKRIQG
                     EGKYVFAYDLDRTAAAQGLYPISLVSYEMACTTYDKAD"
BASE COUNT          378 a          621 c          589 g          333 t
ORIGIN      
        1 ccctgctcgc cacccagtcg cccgtagcga cggacctcag gctgatttca gccctgctcc
       61 acgtgatcaa gaacgcggag cggatggggg accagtgcgt gaacatctgc aagctgatcc
      121 cgatcgtcgg ccatgaaccg ccagccgacg agaagatgat ctcgctgatc ctcgcgatgg
      181 gaacccaggc ccgcagccag atcgtccagg cgcagaaggc cttcgccgac cgtgacgaag
      241 cggcggccca ggatctggtc aggcaggacg atctgattga caagctgaac cgcgaatgct
      301 tcaccctggc gatcgaactc ggcgacgagc ccgaccggcg cgaatgggcg atgaccatgc
      361 tgctggccgc ccgcgccatc gagcggatcg gcgacaatgc ggtcgatgtg ggggaacagg
      421 tggccttcgt ggtcaccgga ctgttccgcg agttcgagga cgcttcacac ccgatctgag
      481 ttggaacagg aattggaagc agcgaagtcc cagctcagcg attcagccga ccacgtcctg
      541 aaggcgggcc cgctggaagt ccggctcgac cagcggaccg tgctcgccga cggcagggga
      601 atcgtcctga ccgtccgcga gttccatctg ctcgcggtac tcgccagccg gcccgagcag
      661 gttgtgagcc gggaagagat ctacgacctg gtctggagcg gccgcatgcc ggaacacgac
      721 cgctcggtcg acgtctatgt ctcgaagcta cggcacaagc tcgaatcggc ccttccccat
      781 tggaacttca tccagaccca catcggcttc ggctactgct tttcgccgaa gccactggct
      841 tcctgaagcc atatttgcgg ggctgttcat ctttttcaca agtttcacaa ggaagtaaca
      901 ccgcgtgatt gcggcccctg ttgactgact ctcattactg cgcaatgcgc cagaagaaac
      961 tcaaactgga ggaaatcttg aacttgaaga agtggtttgt cgcggtaccc ctcaccctgg
     1021 ctctggcctt cggtgttgcc gcgtgcggaa gcagcgattc ggacagcggc ggctcgagcg
     1081 gtgacaccgt ctccggcgaa atcgccggcg ccggctcaag cgctcaggag gcggcaatga
     1141 acggttggat cgccgccttc caggacgcca atcccgacgc gaccctttcc tacgacccgg
     1201 ttggttccgg tggcggccgc gagcagttca tcgccggcgg cacctccttc ggcggcaccg
     1261 actcgccgat ggacgagacc gaactggccg acgtcgccaa gcgttgcgac ccgggcgatt
     1321 acatcgagat cccggtctat atctcgccga tcgccatggc ctacaacatc gacggggtcg
     1381 atgacctgaa tctctccgct gataccgctg cccagatctt cgcgggcgac atcaccaagt
     1441 ggaacgatcc gcccatcgcc gccgacaacc cgaatgcgga cctgcccgat caggacatcg
     1501 tggtggtcca tcgctcggat gaatccggca cgaccgagaa cttcaccgac tacctgcacc
     1561 agaactcgcc gaagtactgg cccgatgagg gcgacggcgt ctggccgctc aagggtggcg
     1621 aggctgccaa cggcacctcc ggtgtggtct cggcgatcaa gtccggttcc ggcacgatcg
     1681 gttacgcgga cctgagccag gtcggtgacc tcaacgtggc caacgtcaag gtcggcgaca
     1741 gctacgtcgc gccgtctgca gaggctgccg cgaagatctt cgaggaatcg aagaggatcc
     1801 agggtgaagg caagtacgtg ttcgcgtacg acctggaccg gacggctgcg gcccagggtc
     1861 tctacccgat ctcgctggtc tcctacgaga tggcctgcac cacttacgac aaggccgatg
     1921 a
//
LOCUS       DAOTIP010000432         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71115_length_1917_cov_3.009667, whole genome shotgun sequence.
ACCESSION   DAOTIP010000432 DAOTIP010000000
VERSION     DAOTIP010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71115_length_1917_cov_3.009667"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..720)
                     /locus_tag="P5138_08295"
     CDS             complement(94..720)
                     /locus_tag="P5138_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HRV60617.1"
                     /translation="MESASIAAHGHVDDHHHGPPEAHQSARIDRTILGILLFILSEVM
                     LFGAFFAAYFFLRVVADPASWPPEGIELPVLIAGINTCILVSSSFTIHYALEAIKHNN
                     HRGLKLGLTTTWLLGATFLFIQINEYIHIGFTPRDGAFGSIFFSLTGLHGAHVFVGLL
                     LLTFANIRAYRGHFGPEQKDHLGVEVPGIYWHFVDIMWIIVFLAVYII"
     gene            complement(723..1625)
                     /locus_tag="P5138_08300"
     CDS             complement(723..1625)
                     /locus_tag="P5138_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60618.1"
                     /translation="MSQPLLIFLNEVAGGRKLLEAAREKAADASYVVVVAPQNQPSVG
                     QLFDPEERAEAARARVDVTLAVLDEFGIEAIGEVLDPEYDLALGDAIRSHRPAEVLLS
                     CTYDFRFGLTRRDLLSWAEITYGDVTNITHIPVRIEDDSISLNVTHTLVVANRTLTSE
                     DLQERLLDRSDERPHRYTIIAPAADDVSEATVARNLASVMAKLYHADIDATGQPMSPD
                     PFDAVKNAIEHYRVDEVLISTLSGEESHWLKQDLVGRVREITDKPVVHHEAGRPPQAV
                     AAVVAEGETADAAAEDSNAEESEA"
     gene            complement(1622..>1917)
                     /locus_tag="P5138_08305"
     CDS             complement(1622..>1917)
                     /locus_tag="P5138_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRV60619.1"
                     /translation="VITIFSFILGAAQLVFIYNVVVSWKYGPKAGPNPWRAKTIEWQV
                     SSPPPVFNFDEIPRVVGGPYEYGTPGARHAIMGGEGEGAEVPETKPSTVTVPS"
BASE COUNT          343 a          575 c          664 g          335 t
ORIGIN      
        1 tgtcatcgct cttgttgccc ccgcaggcag ccagtcccag cgccccgaca cagagacaca
       61 ggaggactgc ggcccggaaa cccatggtcc gggtcagatg atgtagacgg cgaggaacac
      121 gatgatccac atgatgtcga cgaagtgcca gtagatccct ggtacctcga cgccgaggtg
      181 gtctttctgt tccggcccga aatggccgcg gtaggcgcgg atgttcgcga aggtcagcag
      241 gagcagaccg acgaagacgt gggcgccgtg aagccccgtg aggctgaaga agatcgaacc
      301 gaatgcgccg tcacgcgggg tgaacccgat gtggatgtac tcgttgatct ggatgaagag
      361 gaaggtcgcg cccagcagcc aggtcgtggt cagaccgagc ttgaggccgc ggtggttgtt
      421 gtgcttgatc gcctcgagcg cgtagtggat cgtgaacgag gacgaaacca ggatgcaggt
      481 gttgatgccg gcgatcagga ccgggagctc gatgccctcc ggcggccagg aggccggatc
      541 ggcaacgaca cggaggaaga agtaggcggc gaagaaggcg ccgaacagca tcacctcgga
      601 gagaatgaaa agcaggatcc cgaggatggt ccggtcgatc cgggcgctct gatgtgcctc
      661 gggcgggccg tgatggtgat cgtccacgtg accgtgggcc gcgatgcttg cgctttccat
      721 ccctatgcct cgctctcttc agcgttcgaa tcttcggccg cggcgtcggc ggtctcgccc
      781 tcggcgacca cggcggcaac ggcctgaggc ggacggccgg cctcgtggtg aaccaccggc
      841 ttgtcggtga tctcgcgaac ccggccgacc aggtcctgct tgagccaatg cgactcctcg
      901 ccactgaggg tcgagatcag cacctcgtcg acgcggtaat gctcgatcgc gttcttgacg
      961 gcatcgaagg gatcggggct catcggctgg ccggtcgcgt cgatgtccgc gtggtagagc
     1021 ttggccatga ccgaggccag gttgcgggcg acggtcgctt cggaaacgtc atcggcggcc
     1081 ggggcgatga tcgtgtaccg gtgcgggcgc tcgtcggagc gatctagcag ccgctcctga
     1141 agatcctcgc tggtcagagt ccggttggcc acgaccaggg tgtgggtgac gttcaggctg
     1201 atcgagtcgt cctcgatccg gaccggaatg tgagtgatgt tggtgacgtc cccgtaggtg
     1261 atctccgccc aagagaggag gtctcgccgg gtcagtccga aacggaaatc gtaggtgcag
     1321 gagagcagca cctcggccgg tcggtggctg cggatcgcgt caccgagggc cagatcgtat
     1381 tcagggtcca gcacctcgcc gatcgcctcg atgccgaact cgtccagcac cgcgagggtc
     1441 acatcgacac gggcccgtgc tgcctcggcc ctttcttcgg gatcgaagag ctggccgacc
     1501 gaaggctggt tctgcggcgc gacgaccacc acgtaggagg cgtcggccgc cttctcccgg
     1561 gcagcctcga gcagcttgcg cccgcccgca acctcgttga ggaagatgag gaggggctga
     1621 ctcatgaagg tacggtcacc gtgctcggct tcgtttcggg cacttccgcc ccttcgcctt
     1681 caccacccat gatcgcgtga cgcgcacccg gggtgccgta ttcgtaggga ccaccgacga
     1741 ctctcgggat ctcgtcgaag ttgaagaccg gcggcggcga ggagacctgc cactcgatcg
     1801 tcttcgcgcg ccaggggttc gggcccgcct tcgggccgta cttccagctg acgacgacgt
     1861 tgtagatgaa gacgagctgg gcggctccga ggatgaagct gaagatcgtg atcacca
//
LOCUS       DAOTIP010000433         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71118_length_1917_cov_2.912997, whole genome shotgun sequence.
ACCESSION   DAOTIP010000433 DAOTIP010000000
VERSION     DAOTIP010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71118_length_1917_cov_2.912997"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1240)
                     /locus_tag="P5138_08310"
     CDS             complement(101..1240)
                     /locus_tag="P5138_08310"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRV60620.1"
                     /translation="MRDVTALEGLLVADFSRVLAGPLVSMTLADFGADVIKVEPPGGD
                     ETRSWSPPVDSEGRATYFLTVNRNKKSVELNLKDEDDLELARELIRRADVLVENFRPG
                     TMDRLGLGYEDSAADNPGLIYCAVSGFGSKGGASLPGYDPLAQAMSGMMSVTGPRDGE
                     PNKVGVALVDVMAGMNATIGVLVALREREKSGLGQKVESNLMSSALAALENQAAAWLN
                     AGEIPERLGNIHPSIEPFTTFDASDGPIMVCVGNDHQFRALCEVIEEPELADDPRYLR
                     NVDRVRNRETLEEELEDCFGHRTVDDWIATLEAAQVPVGPVNTIDKGFEAGEELGLDP
                     VDEIEGIRTPSSPISLSRTPAETRLPPPGENAHAEEIRAWLREGR"
     gene            complement(1312..>1917)
                     /locus_tag="P5138_08315"
     CDS             complement(1312..>1917)
                     /locus_tag="P5138_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRV60621.1"
                     /translation="LAALHDRKTLREGQRVEINLLSTAIAALANQSSAFLNTGKSPSR
                     LGNVHPSIEPFSTYRTATGPIMICAGNDRQFRQLALTIGAPGLAEDPRFASNLDRVEN
                     REVLREELEHRLGLDSVEYWSEALGDAGVPAGPVNDIGGGFALAESLGLDPVTELDGT
                     RTTSSPIRLSSSPPEKRIPPPALDQHGDEIRDWLRAPRDED"
BASE COUNT          300 a          628 c          633 g          356 t
ORIGIN      
        1 gtttcttcga gaagcttcag atccacacag gaagtgtggc acggggcccg tggcgcgggc
       61 cccgaggcat ttcagcgctt cacgggctgc gtttgcggga ctaccggcct tcccgcagcc
      121 aggcccggat ctcctcggcg tgggcgttct cccccggcgg gggaaggcgc gtttcggccg
      181 gggtgcgact caggctgatc ggcgatgacg gagtgcggat gccttcgatc tcgtcaaccg
      241 ggtcgagacc cagttcctcc ccggcctcaa agcccttgtc aatggtgttg acgggaccca
      301 ccggcacctg tgccgcttcc agggtcgcga tccagtcatc caccgtgcgg tggccgaagc
      361 agtcctcgag ttcctcttcc agcgtttccc ggttgcgaac ccggtcgacg ttgcgcaggt
      421 agcggggatc gtcggcgagc tcgggttcct cgatgacttc acaaagcgcc cggaactgat
      481 ggtcgtttcc gacacagacc atgatcggtc cgtccgaagc gtcaaacgtg gtgaagggtt
      541 cgatgctggg atggatgttc ccgagccgct cggggatctc ccctgcgttc agccaggccg
      601 cggcctggtt ttccagcgcg gccagggccg aactcatcag gttgctctcc accttctgtc
      661 ccaggccgga tttctcacgc tcccggagcg cgacgagcac cccgatggtg gcgttcattc
      721 cggccatcac gtcgaccagg gcgaccccga ccttgttggg ctcgccgtcg cggggaccgg
      781 tcaccgacat catcccgctc atcgcctggg ccaaagggtc gtagcccggc aggcttgctc
      841 cacccttgga gccgaaaccg ctgaccgcgc agtagatgag gccggggttg tccgccgccg
      901 aatcttcgta acccagtccg agacggtcca tcgttccggg acggaagttc tcgaccagca
      961 cgtcggcacg ccggatcagt tcccgggcca gttcgagatc gtcctcgtcc ttcaggttga
     1021 gctcgaccga cttcttgttg cggttcacgg tcaggaagta ggttgcccgt ccttccgaat
     1081 cgaccggagg ggaccaggac ctggtttcgt cgccgccggg cggctcgacc ttgatcacgt
     1141 ccgcgccaaa gtccgccagg gtcatcgaca ccagcggtcc cgcgaggacc cggctgaaat
     1201 ccgctacgag caatccttcg agagcagtca cgtcccgcat aatggcgatc ctgctccccc
     1261 ggtgtccagc accgtggtca ggcgaaggcc agattcacgg tctgtcggcg gtcaatcttc
     1321 gtcgcggggg gcccgcaacc agtcccggat ttcgtcgcca tgctggtcga gagcaggagg
     1381 cggaatcctc ttctcgggcg ggctggacga gagcctgatc ggtgacgaag tggtcctggt
     1441 tccgtcgagt tcggtcaccg gatcgaggcc gagcgactcg gccagggcga aaccgccgcc
     1501 gatgtcattg accggcccgg ccggcacgcc cgcgtcaccc agcgcctccg accagtactc
     1561 gaccgagtcg agcccgagcc ggtgctcgag ctcctccctg agcacctccc ggttctcgac
     1621 ccggtcgaga ttgctggcga aacggggatc ttcggccagg ccgggggcac caatcgtcag
     1681 cgcgagctgc cggaactggc ggtcgttccc ggcacagatc atgatcgggc cggttgccgt
     1741 gcggtaggtc gagaaaggct cgatgctggg atggacgttg cctagccggg aaggggactt
     1801 tccggtgttg aggaaggctg agctctggtt ggccagggcg gcgatcgcag tcgagagcag
     1861 gttgatctcg acgcgctgtc cctcccgcag ggtcttgcgg tcatgcaggg cagcgag
//
LOCUS       DAOTIP010000434         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71571_length_1908_cov_2.689692, whole genome shotgun sequence.
ACCESSION   DAOTIP010000434 DAOTIP010000000
VERSION     DAOTIP010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71571_length_1908_cov_2.689692"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="P5138_08320"
     CDS             <1..1040
                     /locus_tag="P5138_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRV60622.1"
                     /translation="EATTRERAREAADRWPVGEPAPAIDQATRVTLEIIMTAVFGVTE
                     PGRLERLRDAVLALISEIGSRRFLLETAIATARRDGFSRQFPRIEARKAAVDSVVLEE
                     IRERRATSESRDDDVLGRLLAVRDDDGKGLGNPELCDQLRLMLLGGTDTTATTIAWAL
                     DFLTHDPAALNELRRSVEAGEDGYLDAVVQETLRLRPAFPFTVRLTKRPLELEGLTIP
                     ENTLVAPFIALVHRRPDLYPDPLAFRPERFLDGRPGTYSWIPFGGGRRRCIGAAMSQL
                     ETAAVLSVLVDELDLNPVCREPASLGRHSVVAVPANGAPVLAKRPRRIRQESAAETVT
                     PETIDLPRTRS"
     gene            1256..1660
                     /locus_tag="P5138_08325"
     CDS             1256..1660
                     /locus_tag="P5138_08325"
                     /inference="COORDINATES: protein motif:HMM:NF037271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60623.1"
                     /translation="MRELDQELTDETDPEATLEIGFAFLVRFIREHPLFDRLLKTEPE
                     TLLPSVTVEGGPFLDFYRELIADSLRPLVRAGSIAPVDLERAAEGVARLAISLILTPS
                     GLVDADDPDAVAAFARDTLLPMLKPQSGDRRV"
     gene            complement(1680..1752)
                     /locus_tag="P5138_08330"
     tRNA            complement(1680..1752)
                     /locus_tag="P5138_08330"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1716..1718),aa:Glu,seq:ctc)
BASE COUNT          335 a          657 c          597 g          319 t
ORIGIN      
        1 acgaggcgac cactagggaa agggcgcggg aagccgctga ccgctggccg gtcggggagc
       61 ccgcgccggc tatcgaccag gccacccggg tcactctgga gatcatcatg accgcggtct
      121 tcggcgtcac cgaaccggga cgccttgaac gccttcgcga cgcggtactt gcgctcatta
      181 gtgagatcgg ttctcgccgc ttcctgctgg agaccgcgat cgccaccgcg aggcgggacg
      241 gcttcagtcg ccagtttccg cgcatcgagg cccgcaaggc ggcggtcgac tcggtcgtcc
      301 tcgaggagat ccgggagcga cgcgcaacat cggagagccg ggacgatgac gtcctgggcc
      361 ggcttcttgc cgttcgggac gatgacggaa agggtctcgg gaatcccgag ttgtgcgacc
      421 agctccgcct gatgctgctt gggggaaccg acacaaccgc gaccacaatc gcctgggccc
      481 tcgatttcct tacccatgat cccgcggctc tgaacgaact tcggcgttcg gtggaagccg
      541 gcgaggatgg ctatctcgac gccgtagtcc aggaaaccct ccgcctgcgt ccagccttcc
      601 ccttcaccgt tcgtttgacc aagcgacctc ttgagctcga aggcctcact atcccggaga
      661 acaccctcgt ggcaccgttc atcgccctgg tccaccgtcg accagacctc taccccgacc
      721 ccctggcctt caggccggaa cggttcctcg acggccgtcc gggcacctac tcctggattc
      781 cctttggcgg tggccggaga cgctgtatag gcgccgcgat gtcgcaactg gaaacagccg
      841 ccgtcctgag cgtgctggtg gatgagctgg acctgaaccc ggtgtgccgc gagccagcat
      901 ctctcggccg ccattcggtc gttgcggttc cggccaacgg agcaccggtc ctggcgaagc
      961 gcccccggag gatccggcaa gaatccgccg ccgagacggt caccccggaa accatcgacc
     1021 tcccccgcac ccgctcgtga ccggatctgc agatctcatt caagaacgcc cgatcgaacc
     1081 aaccgaccgc atagcaaagg cggcgctggc gcagttcacg gacttcggca tccgccgaac
     1141 cagcgtcgag gacatcgccg ccagggccgg gctctcccgg gtcaccgtct accgaaacgt
     1201 cggcagcagg gacgaggtga tccggctggt tctgagaacc gaggctcaac gagcaatgcg
     1261 cgagctcgac caggagctca cggacgaaac cgatccggaa gccacactcg agatcggttt
     1321 cgcttttctc gtacggttca tccgcgagca ccctctgttc gaccgactcc tgaaaaccga
     1381 gccggaaacc ctgctgccga gcgtaaccgt ggagggcggt ccgttcctcg acttctaccg
     1441 tgagctcatc gccgacagcc tgcgccccct cgtcagggct ggcagcatcg ctcccgtgga
     1501 cctcgagcgc gccgccgagg gagtagcgcg gctggcgatt tccctcatcc ttacgccaag
     1561 cgggctcgtc gacgccgacg atcccgatgc ggttgcggcc ttcgcccgag ataccctcct
     1621 gccgatgttg aaaccgcagt caggcgatcg gcgagtctga tcaagaagga cagagaaggt
     1681 accgctacgg ggattcgaac cccggtttcc ggactgagaa tccggcgtcc tagacccctg
     1741 gacgatagcg gcgcgtggcg ggcattctaa caattcgggt gtcgggttga tcgtgaggca
     1801 gctcacgtgg cgcgcccccg ccgaccggct cgattgctcc ggagccggag tgccccggag
     1861 cgcctgaagc cggtagagtt tcgcctccgc gcggctgtgg cggaactg
//
LOCUS       DAOTIP010000435         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71627_length_1907_cov_2.676566, whole genome shotgun sequence.
ACCESSION   DAOTIP010000435 DAOTIP010000000
VERSION     DAOTIP010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71627_length_1907_cov_2.676566"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="P5138_08335"
     CDS             complement(<1..362)
                     /locus_tag="P5138_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV60624.1"
                     /translation="MIFADTSAVMKLYLEEEGSAEMREFPVLIVSAMCRVEGTSAIWK
                     KLRLGDLDASESEQLIRRFQFDLMPESTGATNLVALPLTGELLRTAAMLVPVHDLTAL
                     DAIQLSRALAAREAEPACS"
     gene            complement(359..664)
                     /locus_tag="P5138_08340"
     CDS             complement(359..664)
                     /locus_tag="P5138_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60625.1"
                     /translation="MAQISLRIPDELAEDIKSDAKAKKLSVNGYITCILRSVSDPEFG
                     GSEAERVRERLRRAGLLEEWVDEPAIEPPSQEELKRIRAKASRGTSLTDLVLEERRR"
     gene            complement(674..1489)
                     /locus_tag="P5138_08345"
     CDS             complement(674..1489)
                     /locus_tag="P5138_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV60626.1"
                     /translation="MSGDPSGLIDQRDPGIFDAHFHVIDPNHPIRANDGFLPDPFTVD
                     DYLERTAGLNVTGGAVVSGSFQGFDQGYLVDALEKLGNSFVGVTQVPAEIADGEILEL
                     DRAGVRAVRFNLRRGGSAELADMVRLARRVYYLAGWHSELYVEGRDLKGLFDRIAALP
                     VACVDHFGLEREGFGDLLRLVESGVKVKASGFGRIDMDPSVAIREIANVDPTALMFGT
                     DLPSTRADRPFRGSDLELFGEVLGSADLEAVLRDNAEALYLKRPRVDAFNHDC"
     gene            1629..>1907
                     /locus_tag="P5138_08350"
     CDS             1629..>1907
                     /locus_tag="P5138_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60627.1"
                     /translation="MQTVNTVTGEIAADELGLTLIHEHFYSSDEAVSVQWPHVRDRQQ
                     EHDLAVESAEAVKGHGVRTVVEPTAMLLGRDVQASRQLAEETGLQIVLC"
BASE COUNT          379 a          605 c          560 g          363 t
ORIGIN      
        1 gagcaggcgg gctctgcctc ccttgcggcg agcgcacgcg agagctggat cgcatcgagt
       61 gccgtcaggt cgtgaacggg aaccagcatc gccgctgttc gcagtagctc cccggtcaac
      121 ggcaaagcaa ccaggttcgt tgcgcctgtc gactcgggca tgaggtcaaa ctggaacctc
      181 cggatgagtt gttcggattc agatgcgtcg agatcgccaa gccttaactt cttccagatg
      241 gctgaggttc cctctacccg acacatggcc gacacgatca gcaccgggaa ttcacgcatc
      301 tcggcggagc cttcctcctc gagatagagc ttcatgaccg ccgacgtgtc ggcaaagatc
      361 accggcgtct ctcttccagg accagatccg tgagggaagt tcccctgctg gcctttgccc
      421 ggatccgctt cagttcctcc tggctcggag gttcgatggc aggctcgtcg acccactcct
      481 caagcaggcc ggccctacga agccgttccc ggacccgctc ggcttctgaa ccaccaaatt
      541 cagggtcgct cactgacctg agtatgcaag tgatgtagcc gttgacgctg agcttctttg
      601 ccttcgcatc agacttgatg tcttccgcca gttcatccgg aattcgaagc gatatctggg
      661 ccatgctttc atgctagcaa tcgtgattga aggcgtcaac gcgcggacgc ttcaggtaga
      721 gggcctctgc gttgtctcgg agaactgcct cgaggtcggc tgatccgaga acctctccga
      781 atagctcaag gtcagagccc ctgaacggac gatcggcccg ggtcgatggc agatcggttc
      841 cgaacatcag cgctgtcgga tcgacgttgg cgatctcccg gattgcgaca gatgggtcca
      901 tgtcgatcct tccgaagcct gatgccttga ccttcacccc tgactcgacc agccgaagca
      961 gatctccgaa accctcccgc tcaaggccga agtggtcgac gcatgcgacg ggaagcgccg
     1021 caatccgatc gaacagaccc ttcaggtccc tcccttcgac gtagagctcg ctgtgccagc
     1081 cggcgaggta atacacacgg cgtgccagac gaaccatgtc ggccagttcg gccgagccgc
     1141 cacggcgaag gttgaacctc accgctcgca cccccgcccg gtcaagttcg agtatctccc
     1201 catccgcgat ttcggcggga acctgggtca ctcccacgaa cgagttcccg agcttttcga
     1261 gggcgtcgac cagatagccc tggtcgaagc cctggaagga gccggaaacg acagcgccgc
     1321 cggtgacgtt cagtccggcc gttcgttcca gatagtcgtc aaccgtgaac gggtccggaa
     1381 ggaaaccatc gtttgcccga atcggatggt tcggatcgat gacatgaaag tgagcatcga
     1441 agatccctgg atcccgttgg tcaatcaggc ccgatgggtc gccgctcatg gaatccgact
     1501 ctacccgggg cctcatcgcc accgatgacc ggccggagaa cggaacccgg tcacttgctc
     1561 atgtgactcc tgacttagca tgggtgccga ggcggacccg ggagccgcaa tcaacgagga
     1621 ggagagccat gcagactgtg aataccgtca ccggtgagat cgcggccgac gaactcggcc
     1681 tgacgctgat ccacgagcat ttctattcat ccgacgaagc cgtctccgtt cagtggcccc
     1741 acgtgcgcga tcggcaacag gaacacgatc tggcggttga atccgccgag gcggtgaagg
     1801 gccacggtgt aaggaccgtg gtcgaaccca cggccatgct gctcggtcgc gacgttcagg
     1861 ccagccggca gttggcggag gaaaccggcc tccagatcgt tctctgt
//
LOCUS       DAOTIP010000436         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71691_length_1905_cov_3.914595, whole genome shotgun sequence.
ACCESSION   DAOTIP010000436 DAOTIP010000000
VERSION     DAOTIP010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71691_length_1905_cov_3.914595"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="P5138_08355"
     CDS             <1..316
                     /locus_tag="P5138_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60628.1"
                     /translation="MKVVGEHGLPWNVTQLGARAEYRFEPEPARNGTQAHEASDPELE
                     RYLHLHALNRGVLITPFHNMALMSPATSPADVDRHTEVFAEAVGDLLGRESAGGSAPK
                     MV"
     gene            339..1166
                     /locus_tag="P5138_08360"
     CDS             339..1166
                     /locus_tag="P5138_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV60629.1"
                     /translation="MESKPTVAVITGASSGIGEGVAWRLARKPGTRLVLVARREDRLR
                     ELARRLPCETTWIAADLTEPDAPGRLLAHVQEHHPPINLLVNNAGAAWRASFGDGGYE
                     NVRRTMEINFDAVLRVTETFLPMLRSTAPSSIVNIASTAGRISRPGAGAYSASKYALI
                     GWNDSLHLEELPNGVHVGLVLPGFIKTEGFPADELTAKFATRWMVSDVSKAAEAVYET
                     AILGKAERYVPRPYALIAVMKILAPGLVRKILSGGAGKAMTTKTGADAAEQAEKTRT"
     gene            complement(1167..>1905)
                     /locus_tag="P5138_08365"
     CDS             complement(1167..>1905)
                     /locus_tag="P5138_08365"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HRV60630.1"
                     /translation="VPRLAGRKMVDFTGPLGWGHSATDLGRVSDTEKGPADGVDMRSR
                     QVLPLIELRAPFTVSRSELEALDRGAVDTDFDSLNDAARQMAVAENAAIFHGLKEAGM
                     EGIAAASSHDPIPLGSNGFQDYPEHIAKAIEVILSSGVEGPYGVALSPECYTGVVESA
                     EKGGYPLLSHIRKILQESGGGPMVWAPGLDGVVVASLRGGDFLFESGQDLSIGYDSHD
                     AEMVNLYIEESFTFRVATPEAAVALPK"
BASE COUNT          317 a          662 c          623 g          303 t
ORIGIN      
        1 tatgaaggtg gtgggagagc atggcctgcc ctggaacgtg acccagctcg gggcccgggc
       61 cgagtaccgc ttcgagccgg agccggcccg caatggcacc caggcccatg aggcttccga
      121 cccggaactt gagcggtacc tgcacctcca cgccctgaac cggggagttc tgatcacccc
      181 cttccacaac atggcgctga tgtctccggc caccagcccg gccgacgtcg accgccacac
      241 ggaggtattc gccgaggccg tcggcgatct gctgggccgg gaatctgccg gcgggtcggc
      301 cccgaagatg gtctaggctg cccttcgtgg aaggcccgat ggaaagcaag ccgacggttg
      361 ccgtcatcac cggagcttcg agcggaatcg gcgaaggggt ggcgtggcga ctggcccgca
      421 agcccggcac ccgtctggtg ctggtcgcca ggcgcgagga cagactgcgg gagctggcca
      481 ggcggctccc ctgcgagacg acctggatcg ccgccgacct gaccgagccc gatgcccccg
      541 gccgcctcct cgcccacgtc caggagcacc atcccccgat caacctgctg gtcaacaacg
      601 ccggcgccgc ctggcgcgcg agcttcgggg atggcggcta cgagaacgtg cggcggacca
      661 tggagatcaa cttcgacgca gtgctcaggg tcaccgagac cttcctgccg atgctgcgct
      721 cgacggcgcc cagctcgatc gtcaacatcg cttccaccgc cggtcggatc tcccggccgg
      781 gcgccggtgc gtactcggcc tcgaagtacg ccctgatcgg atggaacgac agccttcacc
      841 tggaggaact gccgaacggg gtccacgtcg gactggtcct gcccggtttc atcaagaccg
      901 agggtttccc ggccgacgag ctcaccgcaa agttcgcgac ccgctggatg gtctccgacg
      961 tctccaaggc agccgaggcc gtttacgaga cggcgattct cggcaaggcc gagcgctacg
     1021 tcccgcggcc ctacgcgctg atcgcggtga tgaagattct ggctccgggc ctggtccgca
     1081 agatcctctc cggcggagcc ggcaaggcca tgaccacgaa gaccggcgcc gacgcggccg
     1141 agcaggccga gaagacccgg acctgactat ttcggcaggg cgaccgcggc ttccggagtc
     1201 gcgaccctga aggtgaagct ctcctcgatg tagaggttga ccatctcggc gtcatggctg
     1261 tcgtagccga tcgaaaggtc ctggcctgat tcgaagagga agtcccctcc gcgcagactc
     1321 gccaccacga ccccgtccag ccccggcgcc cagaccatcg ggccgccgcc actttcctgc
     1381 aggatcttgc ggatgtggga gagcaacggg tagccgccct tctcggcgct ctcgaccact
     1441 cccgtgtagc actccggact gagcgccact ccgtagggcc cttcgacgcc ggaggagagg
     1501 atcacctcga tcgccttggc gatgtgctcc ggatagtcct ggaagccgtt gcttccgagc
     1561 gggatcgggt catggcttga ggcagcggca attccttcca tgccggcttc cttcaggccg
     1621 tggaagatcg ctgcgttctc ggccacggcc atctgccggg cggcgtcgtt gagcgaatcg
     1681 aagtcggtgt cgaccgctcc gcgatcgagc gcctcgagct cggaacgact gaccgtgaac
     1741 ggcgccctca attcgatcag cggcagtacc tggcggctgc gcatgtcaac gccgtcggcc
     1801 ggtccttttt ccgtgtccga aacccggccg agatcggttg ccgaatgccc ccagccgagg
     1861 ggaccggtga agtcgaccat cttgcggccg gcgagccgcg ggacc
//
LOCUS       DAOTIP010000437         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71695_length_1905_cov_3.831892, whole genome shotgun sequence.
ACCESSION   DAOTIP010000437 DAOTIP010000000
VERSION     DAOTIP010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71695_length_1905_cov_3.831892"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="P5138_08370"
     CDS             <1..989
                     /locus_tag="P5138_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRV60631.1"
                     /translation="GHPDAQYEDAGAKVGSRADVLAADMVLHVAPLSESEIGELKQGQ
                     YLISHLSPLTSAETNKALASAGVTALAMELIPRTTRAQAMDALSSQATAAGYAATLIA
                     ARESGSFWGMLTTAAGTIRPARVLVLGAGVAGLQAIATAKRLGAIVTGFDIRRAAWEQ
                     IASLGGRPLELDFIPDAEGEGGYARPLTDEENQQVRDAIDENAARQDVIITTAAIPGR
                     PAPTLITAAGVANMGPGSVILDLAAETGGNCELTEPGQTVVKDGVKIVGPKNLASELP
                     GHASQLYAKNLENLLGLMITEEGGLTLNFEDDIIDAATVTHEGEIRGERTAR"
     gene            989..1327
                     /locus_tag="P5138_08375"
     CDS             989..1327
                     /locus_tag="P5138_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRV60632.1"
                     /translation="MDLVTEITILVLAVFVGFEVISKVPTTLHTPLMSQTNAIHGIVL
                     LGGLIVVGYSEDFLGQLIGFIAIVFGTINIVGGFMVTDRMLEMFGPKKKKPEPAAEEP
                     KATDSTEAGS"
     gene            1324..>1905
                     /locus_tag="P5138_08380"
     CDS             1324..>1905
                     /locus_tag="P5138_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRV60633.1"
                     /translation="MSPLLAALAPDSSLVAILYIVSFSLFIIGIKQGTHPTTARRGNM
                     VAAVGMAIAVITTLCLKGIGNWGIIIAGILVGTIVGAVASRRVKMTEMPQMVALYNGL
                     GGGAIALIGWSEYRHWIHIDNQLSSPHSFTEGAWGAYNAGLDANFVGGTIKTLVSVSV
                     PLEVLIPTLLAMVIGSISFWGSNVAFGKLQELIP"
BASE COUNT          337 a          663 c          590 g          315 t
ORIGIN      
        1 ccggtcaccc tgacgcccag tacgaggatg ccggggcgaa ggtcggctcg cgcgccgacg
       61 ttctcgccgc cgacatggtc ctccacgtcg ccccgctctc ggaaagcgag atcggcgaac
      121 tgaaacaggg ccagtacctg atcagccacc tctcgccgct gacttcggcg gagaccaaca
      181 aggcgctcgc ctcggccgga gtcaccgctc tggccatgga gctcattccg cgcaccacgc
      241 gcgcccaggc gatggacgca ctctcatcgc aggcgactgc tgccggttac gccgcgaccc
      301 tgatcgccgc ccgtgagtcg ggcagcttct ggggcatgct gaccactgcc gccggcacga
      361 tccggccggc ccgcgttctg gtactcggcg ccggtgtcgc cggtctgcag gcaatagcca
      421 cggccaagcg gctcggcgcg atcgtcaccg gcttcgacat ccgtcgcgcc gcctgggagc
      481 agatcgcctc gctcggcggc cgtccactcg aactcgactt cattcccgac gcggagggtg
      541 agggcggtta cgcccggccg ctcaccgacg aggagaacca gcaggtccgc gacgcgatcg
      601 acgagaacgc cgccaggcag gacgtgatca tcaccacggc agccattccc ggccggccgg
      661 cgccgaccct gatcaccgcc gccggtgtgg ccaacatggg tcccggatcg gtgattctcg
      721 acctggccgc cgagaccggc ggaaactgcg aactgaccga acccgggcag accgtggtca
      781 aggacggcgt gaagatcgtc gggccgaaga acctcgcctc cgagctgccc ggtcacgcct
      841 cacagcttta cgccaagaac ctcgagaacc tgctcggcct gatgatcacc gaggaaggtg
      901 gcctcaccct gaacttcgag gacgacatca tcgacgccgc gacggtcacc cacgagggtg
      961 agatccgcgg agagaggacc gcccgctgat ggacctcgta accgagataa ccatcctcgt
     1021 gctggcggtc ttcgtcggtt tcgaggtgat ctccaaggtt ccgaccacgc ttcacacgcc
     1081 gctgatgtcg cagaccaacg cgatccacgg catcgttctg ctgggcggcc tgatcgtggt
     1141 cggttactcc gaagacttcc tcggccagct gatcggcttc atagcgatcg tcttcggcac
     1201 gatcaacatc gtcggcggct tcatggtcac cgaccgcatg ctggagatgt tcggtccgaa
     1261 aaagaagaag cccgaaccgg cggctgagga accgaaagcc accgattcga cggaggccgg
     1321 ctcgtgagtc cacttctcgc tgcgctggcg ccggattcgt cgctcgtcgc aatcctctac
     1381 atcgtcagct tctcgctgtt catcatcggc atcaagcagg gcacccaccc gacgacggcc
     1441 cgccggggca acatggtcgc cgcggtcggc atggcgatcg ccgtcatcac cacgctctgc
     1501 ctgaagggaa tcggcaactg gggcatcatc atcgccggca tcctggtcgg cacgatcgtc
     1561 ggggcggtcg cgtccaggcg ggtcaagatg accgaaatgc cgcagatggt cgccctctac
     1621 aacggcctcg gcggcggtgc catcgccctg atcgggtggt ccgagtaccg gcactggatc
     1681 catatcgaca accagctcag ttctccccac agcttcacgg aaggtgcctg gggcgcctac
     1741 aacgccggcc tcgacgcgaa ctttgtgggc gggacgatca agaccctggt cagcgtctcg
     1801 gttccgctgg aggtcctgat cccgaccctg ctggccatgg tcatcggctc gatctctttc
     1861 tgggggtcga acgtcgcctt cggcaagctg caggaactga ttcca
//
LOCUS       DAOTIP010000438         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71916_length_1901_cov_2.493499, whole genome shotgun sequence.
ACCESSION   DAOTIP010000438 DAOTIP010000000
VERSION     DAOTIP010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71916_length_1901_cov_2.493499"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="P5138_08385"
     CDS             <1..537
                     /locus_tag="P5138_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60634.1"
                     /translation="AIGFALAGPGLGLTIAGLSLVGLVIVAMLVIPRDPIGRTPHPEL
                     ARRLLVVTTFPIEDAVSIQRVADEVRLGFDENEAEVRLLTPAANTFLRRWATDLERAR
                     DNAQRDLVVSVASLTLAGVDASAAVGDEDLVQAVEDELVTYDATEVFLLTRGDPDSEA
                     ARQLRERLLPRFHHVDLR"
     gene            549..1280
                     /locus_tag="P5138_08390"
     CDS             549..1280
                     /locus_tag="P5138_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV60635.1"
                     /translation="MSAVIEVKGLTKDFGSTRALDGLDLTVEPGQVHGFLGPNGSGKS
                     TTIRVLLGLIRATGGTARLFGEDPWKNAVSLHRRIAYVPGDVNLWPNLTGGETIDMLG
                     SLRGGLDPKRRADLVDRFELELTKKASTYSKGNRQKVALVAALAAEAELFIFDEPTSG
                     LDPLKEEVFHECVREITGEGRTILLSSHILGQVQGLATTFTIIRNGKTVETGTLAELR
                     QIAVSAGRDPESLSLEDLFLSIYAS"
     gene            1280..>1901
                     /locus_tag="P5138_08395"
     CDS             1280..>1901
                     /locus_tag="P5138_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60636.1"
                     /translation="MTGFAGTADAIRFALKRDRFILPICVAGLIGWMVIYVLSYNDLY
                     GTQAELNALYKSVAGNPALVAMSGPTGGLRSLGGALSWDSTPALSIIGGVFAMFSVIR
                     HTRAEEEDGRTELLLTSGSGRLAPLAAAVIVTSFALVLASIGYVIALSVGGFELGPSI
                     LLSLSLLGFGLVITGTTAVFAQVTSKARAARGLVGIVIGVAWLLRAI"
BASE COUNT          289 a          664 c          615 g          333 t
ORIGIN      
        1 gcgatcggtt tcgcgcttgc cggcccgggg ctgggcctga ccatcgccgg tctctcgctg
       61 gtcggcctgg tcatcgtggc gatgctcgta atcccacggg atccgatcgg ccggacgcca
      121 catcccgaac tcgcccggcg gctgctcgtg gtgaccacct tcccgatcga agacgccgtc
      181 tcgatccagc gggtagccga tgaggtccgg ctcggcttcg acgagaacga agccgaggtt
      241 cgcctgctca ccccggcggc gaacactttc ctgcgccgct gggccaccga cctcgagcgg
      301 gcccgtgaca acgcccagag ggacctggtc gtatcggtcg cctccctcac cctcgccggt
      361 gtcgatgcct ccgctgccgt gggtgatgag gatctcgtcc aggcggtcga ggacgaactc
      421 gtcacctacg acgcgaccga ggtcttcctg ctgacccggg gcgaccccga ctcggaagca
      481 gcccggcagc ttcgcgaacg cctgctgccc cgcttccacc acgtcgacct gcggtagttt
      541 ccggggccat gagtgccgtc atcgaagtga agggcctgac caaggatttc ggcagcaccc
      601 gtgcgctcga cggtctggac ctcacggtcg aaccgggcca ggtccacggt ttcctcggcc
      661 cgaacggctc cggcaagtcg accacgatca gggtcctgct cggtctgatc cgggcgacgg
      721 gcggcacggc ccgcctgttc ggggaggacc cctggaagaa cgccgtttcc ctgcaccgcc
      781 ggatcgccta tgtgccgggt gacgtgaatc tgtggccgaa cctgaccggc ggcgagacga
      841 ttgacatgct cggctccctg cggggcgggc ttgatcccaa acgcagggcg gatctggtcg
      901 accggttcga actggaactg accaagaagg cctcgaccta ttcgaagggc aaccgccaga
      961 aggtcgccct ggtggcggcc cttgcggctg aagcggagct cttcatcttc gacgagccga
     1021 cctccggcct cgacccgctc aaggaggagg tcttccacga atgtgtgcgg gagatcaccg
     1081 gagagggccg caccatcctg ctctccagcc acatcctcgg acaggtccag gggctggcca
     1141 cgactttcac gatcatccgc aacggcaaga ccgtcgagac gggaacgctc gcggagttgc
     1201 ggcagatcgc cgtcagcgca ggacgggacc ccgagtcgct cagcctcgag gacctgttcc
     1261 tcagcatcta cgcgagttga tgaccggttt cgccggaacc gccgacgcga tccgcttcgc
     1321 actcaagcgg gaccgtttca tcctgccgat ctgcgtggcc ggcctgatcg gctggatggt
     1381 gatctacgtg ctcagctaca acgacctcta cggcacccag gccgaactga acgcgctcta
     1441 caagagcgtg gccggcaatc ccgccctggt cgcaatgtcg ggaccgaccg gcggactcag
     1501 gagcctgggc ggcgccctct cctgggacag cacccctgcc ctctcgatca tcggcggtgt
     1561 cttcgcaatg ttcagcgtga tccgccacac ccgcgccgag gaggaggacg gccgcaccga
     1621 gctgctgctc acctcgggtt cgggtcggct ggccccgctg gcggcggcgg tgatcgtcac
     1681 ctccttcgcg cttgttctgg cctcgatcgg ctacgtcatc gcgttgtcgg tcggcggatt
     1741 cgaactcggc ccgtcgatcc tgctttcgct gtcgctgctc ggtttcgggc tggtcatcac
     1801 cggtaccacc gccgtcttcg cccaggtgac gagcaaggcc cgggccgccc ggggacttgt
     1861 cggcatcgtg atcggggtcg cctggctgct ccgggcgatc g
//
LOCUS       DAOTIP010000439         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_71989_length_1899_cov_3.956616, whole genome shotgun sequence.
ACCESSION   DAOTIP010000439 DAOTIP010000000
VERSION     DAOTIP010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71989_length_1899_cov_3.956616"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="P5138_08400"
     CDS             <1..710
                     /locus_tag="P5138_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60637.1"
                     /translation="DAPSPGLVGGNQSLALALAGRLGERVHLNEPAEFINWSESGVAV
                     CCESGAIFDAERCVLAVPGSVIDRIQFAPALPENKAEAFQSLRYGHAAKLFVPLLEPA
                     PVGAVMNVPERWWCWVQSGKDGQPLRAVHCFAGSPRALEGLAISAGPNRWLDSLASLR
                     PELSLDPDGAVLSTWDDDPRVGAAYSISPSAEVTASLIEPVGPFRFVGEHTAGPFSAL
                     MEGAVRSGRRADVTDAG"
     gene            complement(732..>1899)
                     /locus_tag="P5138_08405"
     CDS             complement(732..>1899)
                     /locus_tag="P5138_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRV60638.1"
                     /translation="EASEGRRVLAIAALPGPLPAADEEPTLPADLKPLGLIVLAERLR
                     DDAEATVDFFHQEAVALKVLSGDAPATVGAIARDTGIARGTTGPALNGEDLPETDEEL
                     LTAIHDSPAIGRISPEGKERVISVLKESGEYVGMIGDGVNDVPALKKSRLAIAQGNGT
                     QMARSVADLVLVSGSFAAVPGMVHEGRQILRNIQRVSRLFISKAMFTAFLLATVAIPT
                     GIFPLLPRQFTLTSSLTIGIPAFFLALAPSAGAWRPEGFLKAIARFSIPVGVATGIGI
                     LASWVIGRHLLDVTLDDSRTVTCATVVTAGLAIVMVLEDEPGKRRLYVAGLCALMAIG
                     FILAVLIPPIRDFFALQTFNGDMLLAWAAGSAIGLTLMAITLKIIGRDNPRGAR"
BASE COUNT          291 a          620 c          692 g          296 t
ORIGIN      
        1 gcgatgctcc ttcacccggg ctggtcggtg gcaaccagtc gctcgcgcta gccctcgccg
       61 ggcgcctcgg cgaacgggtc cacctgaacg aacccgccga gttcatcaac tggtccgaga
      121 gcggggtcgc agtctgctgc gaatccgggg ccatcttcga tgccgagcgc tgcgtgctgg
      181 cggtgcccgg atcagtgatt gaccggatcc agttcgctcc agcgctgccc gagaataagg
      241 ccgaagcatt ccagtcgctc cgctacggcc acgccgccaa gctcttcgtt cccctgctgg
      301 aaccggcccc ggtcggggcc gtgatgaacg tgcccgaacg ctggtggtgc tgggttcagt
      361 ccgggaagga cggccagccc ctgcgggcgg ttcactgttt cgccggatcg ccccgggccc
      421 tggagggact ggcgatcagc gcaggcccca accggtggct cgactcgctg gcctcgctca
      481 gaccggagct ctcgctcgat cccgacggtg ccgtcctctc gacctgggac gacgatcccc
      541 gggtcggggc ggcctattcg atctcgccct cggccgaggt gaccgcatca ctgatcgagc
      601 cggtcggccc gttccgcttc gtcggcgaac acaccgccgg gccgttctcg gctctcatgg
      661 aaggggcggt caggagcggc cggcgggcgg acgtcaccga tgccggctga gttccggacg
      721 tcgaaccgaa atcagcgggc gccgcggggg ttgtcgcggc cgatgatctt gagggtgatt
      781 gccatcaggg tgaggccgat cgccgatcct gctgcccagg cgaggagcat gtctccgttg
      841 aaggtctgaa gggcgaagaa gtctcggatc ggggggatca ggaccgcgag gatgaagccg
      901 atcgccatca gggcgcagag gccggctacg tagaggcggc gcttgcccgg ttcgtcttcg
      961 aggaccatca cgatcgccag gccggcggtg accaccgtgg cgcaggtgac ggttcgggaa
     1021 tcgtccagcg tcacgtccag caggtgtcgg ccgatcaccc acgaggcgag gatgccgatg
     1081 ccggtcgcga cgccgaccgg gatcgagaag cgggcgatcg ccttgaggaa gccctccggc
     1141 cgccaggcgc cggcgctcgg ggccagggcg aggaagaagg ccgggatgcc gatggtcagc
     1201 gacgaggtga gcgtgaactg acggggcagc agcgggaaga tgccggtcgg gatcgcgacc
     1261 gtggccagca ggaaagccgt gaacatcgcc ttcgagatga agaggcgcga gacccgctgg
     1321 atgttgcgga ggatctggcg gccctcgtgg accattcccg gcacagcggc gaagctgccc
     1381 gagaccagca ccaggtcggc gaccgaacgg gccatctggg tgccgttgcc ctgggcgatc
     1441 gccaggcggg acttcttcag ggccgggacg tcgtttaccc cgtcaccgat catgccgacg
     1501 tactcgcccg actccttcag gaccgagatg acccgctcct tgccctccgg ggagatccgg
     1561 ccgatcgccg gcgaatcgtg aatcgcggtc agcagttcct cgtcggtttc gggcaggtcc
     1621 tcgccgttca gcgccgggcc ggtggtgccg cgggcgatgc cggtgtcacg ggcgattgct
     1681 ccgaccgtgg ccggggcgtc accggacagc acctttaggg cgaccgcttc ctgatggaag
     1741 aagtcgaccg tggcctcggc gtcgtcgcgg agcctttcgg cgaggacgat caggccgagt
     1801 ggcttcaggt cggccggaag ggtcggttcc tcgtcggcgg ccggaagcgg gccgggcagg
     1861 gcggcgatcg ccagcacccg gcggccctcc gaagcttcg
//
LOCUS       DAOTIP010000440         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_72211_length_1895_cov_3.274457, whole genome shotgun sequence.
ACCESSION   DAOTIP010000440 DAOTIP010000000
VERSION     DAOTIP010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72211_length_1895_cov_3.274457"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="P5138_08410"
     CDS             complement(<1..633)
                     /locus_tag="P5138_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60639.1"
                     /translation="MKTLFATFIAVTAFAVPGAAAAGTPEEDPAEIVFENGGRIVSIK
                     ADGSGRTVLTRKNAWVGQPWGINDIGPLVSPDGNSILFNRYNDGFNEGIPTGLMLMNR
                     DGSGIHPAYEPPDGAELFSGNWSSDGERIYLIEYSEESTPTGGIGTTTLFSVKTDGTD
                     RKVITKAVFEYDEEDDKVTGQKWIPVDVASTQDGSRLLITTYNFFTDKPTR"
     gene            complement(630..>1895)
                     /locus_tag="P5138_08415"
     CDS             complement(630..>1895)
                     /locus_tag="P5138_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60640.1"
                     /translation="RSYAGGMPKDDWNFEAARLAISPDGRNLLVTAMDIWSESGYRLG
                     LVNLETGKRKLIAKGAHSGAWSPDGNSFVFVKDRRKAEVCDWDFECTPSGDLFTAKAD
                     GTGIRRLTDTRRDEATPSFSPDGSRIVFSGTFHRPSDRTTAELFSMSADGGCPVRITN
                     GSPASLDPFFVPGSGDYAYPGGCEPVDRPALVETGLNRVKRKDFGRRLWLGKESSQGL
                     LSMDFDYVFLSYTLYGDCSESRAKDCPPGATVFNGSVCLNPGSWAEKIADLVRSKRKM
                     KRRGVWVEQSRVNGNSSGRATWVYSGRWITTITGAVGPGNDGFSRLGYAAQLDLVDEL
                     RPRGVKPGARLPKLLIPTFDFQLARFYRRQVERSSVTEVAKKFRVSRRKVRDLANFTR
                     NLRRLGPYGRTRCPDASFPVETDSGSDRS"
BASE COUNT          338 a          599 c          606 g          352 t
ORIGIN      
        1 ccgggtcggc ttgtcggtga agaagttgta ggtcgtgatc aggagccgac taccgtcctg
       61 ggtggaagcc acgtcgaccg gaatccattt ctgcccggtc accttgtcgt cctcctcgtc
      121 gtactcgaag acggctttcg tgatgacctt gcgatcggtg ccgtccgtct tcaccgagaa
      181 gagcgtggtg gtcccgatac cgccagtcgg ggtcgattcc tcgctgtatt cgatgagata
      241 gatgcgttca ccgtccgacg accagttgcc cgagaagagt tccgcgccat cgggcggttc
      301 ataggcggga tggatcccgg acccgtcgcg gttcatcagc atgaggccgg tcgggatccc
      361 ctcattgaac ccgtcgttgt agcggttgaa caggatcgag ttcccgtccg gcgaaaccag
      421 cgggccgatg tcgttgattc cccagggctg cccgacccag gcgttcttcc gagtcaagac
      481 ggtccgtccg gatccatcgg ccttgatcga aacaatccgg ccgccgttct cgaagacgat
      541 ctcggccgga tcctcctccg gggtgccagc cgctgcggca cccgggacag cgaacgcggt
      601 gacggcgatg aatgtggcga agagggtctt catgagcggt cggaccccga gtccgtttca
      661 accgggaagc ttgcatccgg gcaacgggtc cggccgtagg gaccgaggcg acggaggttg
      721 cgcgtgaagt tagccaggtc acggaccttc cgcctgctca cccggaactt cttcgccact
      781 tccgtgaccg aggatcgctc gacctgccgc ctgtagaagc gggccagctg gaaatcgaac
      841 gtggggatca gcagcttcgg cagacgggca cccggcttca ctccgcgagg acggagttca
      901 tcgacgaggt cgagttgggc ggcgtagcca agccgggaaa aaccgtcgtt gccggggccg
      961 acagcgccgg tgatcgtggt gatccagcgg cccgaataga cccaagtcgc tcttccggaa
     1021 gaattaccgt tgacccggct ctgctccacc cagactcccc gccgcttcat ctttcgcttc
     1081 gaacggacca gatcggcaat cttctccgcc cagctgcccg ggttgaggca caccgatccg
     1141 ttgaacacgg tagccccggg tggacagtcc ttcgccctgg attcagagca gtctccgtag
     1201 agggtgtagg agaggaacac gtagtcgaag tccatggaaa gcaggccctg actcgactct
     1261 ttgccaagcc agagacgacg gccgaagtct ttccgcttca cccggttcag cccggtctcg
     1321 accagcgccg gccggtcaac cggctcgcag ccgccgggat atgcgtaatc gccggaaccg
     1381 ggcacgaaaa agggatcaag gctggccggg gaaccattgg tgatcctgac cgggcaacct
     1441 ccgtcagcgc tcatcgagaa cagctcggcg gtggtccggt cggagggccg gtggaaagtg
     1501 ccgctgaaga cgatccggct gccgtcgggc gagaagctcg gggtggcctc gtcccgccgg
     1561 gtgtcggtga gacgcctgat gccagtgccg tcggccttcg ccgtgaagag gtcaccgctc
     1621 ggcgtgcatt cgaaatccca gtcacagact tcggcttttc tgcggtcttt gacgaagacg
     1681 aaactgttcc cgtccggtga ccacgcgccg gaatgggctc ccttggcgat cagcttccgc
     1741 ttgccggtct ccagattcac cagccccagc cggtaaccgg actcgctcca gatgtccatg
     1801 gcggtcacca gcaggtttct gccgtcgggc gagatagcaa gacgggcggc ctcgaagttc
     1861 cagtcgtcct tcggcatgcc gccggcgtaa ctgcg
//
LOCUS       DAOTIP010000441         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_72212_length_1895_cov_3.258696, whole genome shotgun sequence.
ACCESSION   DAOTIP010000441 DAOTIP010000000
VERSION     DAOTIP010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72212_length_1895_cov_3.258696"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="P5138_08420"
     CDS             <1..269
                     /locus_tag="P5138_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60641.1"
                     /translation="SVTIWGDSIDSNMGQLLVRNIDDELVRELKLRAAGNGVSAEEEH
                     RRILRAALSADDDREFLEHLMAMPDVGEDSDFEREREFPRETDL"
     gene            266..682
                     /locus_tag="P5138_08425"
     CDS             266..682
                     /locus_tag="P5138_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV60642.1"
                     /translation="MSYLLDTNVISELRKGKAADPAVTGWIEAMHGEGFFLSVLVVGE
                     LRKGIERLAGRDPESARALDDWLRGVQRRFEGRVIPIDTDIAEEWGRMSASDSPSVID
                     GLLAATALVKDLTLVTRNTADVERTGVALVNPFVLK"
     gene            complement(695..1648)
                     /locus_tag="P5138_08430"
     CDS             complement(695..1648)
                     /locus_tag="P5138_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRV60643.1"
                     /translation="MRAASYEEYGPPEVVHITELPAPDPGKGEVLVRVKSTAVTVADA
                     RIRAARFPIGFVVPARLTFGLRRPRRHVLGSSFSGTVESVGSEVEGFAPGDEVCGMNG
                     IRMGAHAEQVSVPAKNLVRKPSSVLHADAAGILFGGTTALYFLRDLGQLEPGQSVLVN
                     GASGSVGTAAVQLALRLGGKVTAVTSSRNLELVTGLGAEQVIDYSQTPVTGIEDQFDV
                     VLDTVGTLDRKSGLKLVREGGVLLLVVATFRETVRPNGKVKSGTTPEHPDDFLYLVEL
                     LEQGAIRNVGDELEGLEQIVAAHAKVDSGHKTGNIVINLPG"
BASE COUNT          333 a          617 c          626 g          319 t
ORIGIN      
        1 attcggtcac gatctggggt gatagcattg atagcaatat gggtcagcta ctggtcagga
       61 acatcgacga cgaactggtc cgggaactca agctccgggc cgctgggaac ggggtgtccg
      121 cagaagaaga gcaccggcgg atcctcaggg cggccctgtc tgccgacgac gatcgcgagt
      181 tccttgagca cctgatggcg atgcccgacg tcggtgaaga ctccgacttc gagcgcgagc
      241 gggagttccc ccgcgagacg gatctttgag ttatctgctc gacaccaacg tcatctccga
      301 gttgaggaag gggaaggctg ccgatcccgc tgtgaccggc tggatcgaag ccatgcacgg
      361 agaaggcttc ttcctcagcg tcctcgtggt cggcgaattg aggaagggca tcgaacgtct
      421 ggctggacgg gatccggaat cggcgcgcgc actagacgat tggctgcgcg gagtccagcg
      481 acgcttcgag ggccgggtca tcccgatcga tacagatatc gccgaagaat ggggcaggat
      541 gagcgcctcg gactcgccct cggtgatcga cggactgctg gccgcaaccg ccttggtgaa
      601 ggacctcacc ctggtgacca gaaacacggc ggatgtcgag cggaccgggg tcgccctggt
      661 caacccgttc gtcctgaagt gaaacgtcgc cggctcagcc gggcaggttg atcacgatgt
      721 tgccggtctt gtggccggaa tcgaccttgg cgtgagccgc gacgatctgc tccagtcctt
      781 ccaactcgtc gcctacgttc ctgatcgccc cctgctcgag cagctcgacc aggtagagga
      841 aatcgtcggg atgctccggg gtggtccctg acttcacctt cccgttcggc ctgaccgtct
      901 cgcggaaggt ggcgacgacc agcagcagta ctccaccttc ccggaccagc ttcaggcctg
      961 acttgcggtc cagggtcccg accgtgtcga gcaccacgtc gaactggtct tcgatcccgg
     1021 tgacgggggt ctggctgtag tcgatcacct gctcggcgcc gaggccggtc accagctcca
     1081 gattgcgtga gctggtcacc gcggtcacct tgccgccgag gcggagcgcc agctgcaccg
     1141 ccgcggtccc gaccgagccg gaggcgccgt tgaccagcac cgactgtccg ggctcgagct
     1201 ggcccagatc acgcaggaag tagagcgccg tggtgccccc gaagaggatc cccgccgcgt
     1261 cggcgtgcaa gacactcgac ggtttccgga ccaggttctt cgccgggacc gacacctgct
     1321 cggcgtgggc gcccatccgg atcccgttca ttccgcagac ctcgtccccg ggagcgaaac
     1381 cctcgacctc gcttccgacc gactcgaccg ttcccgagaa ggagctgccg agcacatgcc
     1441 gccgcggccg cctcagcccg aaggtgagcc gggcaggaac cacgaacccg atcggaaagc
     1501 gggcggcacg gatcctcgcg tcggccacgg tcaccgcggt ggacttcacc cggaccagga
     1561 cctcgccctt gccggggtcg ggggctggca gttcagttat gtggaccacc tcgggcggtc
     1621 cgtactcctc gtagctcgcc gctctcatcg cttcaactta tcgtccaccg atgcggcagc
     1681 cgggggagac attttccggc cgcgcccttt accgggcatg gcagaaaaca gtcagcagcg
     1741 acgggaaggc aaaggggagg gcgagccgac cgaagaggag ctcgcggcct gcgccctggt
     1801 ggtggagctg cttcgctcgg gccggatact cacctacatc ggcgacatcc tggtcgagcg
     1861 ggtcgacgag atcgacttcc ccggggagtc cgagg
//
LOCUS       DAOTIP010000442         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_72643_length_1887_cov_2.950873, whole genome shotgun sequence.
ACCESSION   DAOTIP010000442 DAOTIP010000000
VERSION     DAOTIP010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72643_length_1887_cov_2.950873"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="P5138_08435"
     CDS             complement(<1..799)
                     /locus_tag="P5138_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60644.1"
                     /translation="MIEPELSGIADEILGSIGQLIPEYAMPLEGSFGKAVYRGVGEAL
                     EQFLVLIRDPDADRRASRKVYVILGRGELRQGRSLDSLQSAYRIGARIAWRRIAAASL
                     EAGLDGKTLSLLAESIFVYIDELSADSTEGYANAQFKQQGSRLRRERQLITLMLREPA
                     PEHEEIEVAARMAVWELPKSLAAVACHERDLAEVTRVLPIGVISATVDGYGCVAIPDP
                     GGPGRLRQIRPLFAGRTSAMGPTVGPNRMAESWQLARTAFEAAAAGVL"
     gene            934..>1887
                     /locus_tag="P5138_08440"
     CDS             934..>1887
                     /locus_tag="P5138_08440"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRV60645.1"
                     /translation="MTQHKMPLLDQIVNLFAVVIPFLATIAAIVLFWNQIVTPLDLAL
                     LAGMYLLTTFGITIGFHRLLTHRSFQTFKPVEYFLAIAGSMAVEGPVNTWVADHRKHH
                     AHTDEEGDPHSPHVGHDGGIKGVLKGLFHAHIGWTFTDHGKAEQQKYAKDLYEDRGMR
                     FISDFFVLWVVLGLLIPAGIGYLVTGTLAGAAGGLLWGGLVRVFFVHHITFSINSVCH
                     FLGTRRFDIDDHSTNVFWLALPSMGEAWHHNHHAFPRSANHGLKKWELDPSAWVITAM
                     EKTGLARKVVRISPERQAEKLAQPEKLAQPEKLAQPEKPRTP"
BASE COUNT          346 a          626 c          565 g          350 t
ORIGIN      
        1 gcaggacacc ggccgcggcg gcctcaaagg cggtccttgc cagctgccac gactcggcca
       61 tccggttcgg ccccacggtc ggacccatgg cgctggtgcg tccggcaaac aggggtctga
      121 tctggcgcag gcgacccggc ccgcccgggt cgggaatggc gacgcagccg taaccgtcga
      181 ccgtggccga gatcacaccg atggggagga ctcgggtgac ctcggccagg tcgcgttcat
      241 ggcaggcaac cgccgccagg cttttcggca attcccagac cgccatccgg gctgccactt
      301 caatctcttc gtgttcgggc gcgggttcgc ggagcatcag cgtgatcagc tggcgctccc
      361 tcctcagcct cgatccctgc tgtttgaact gagcgtttgc gtaaccctcg gtggagtcgg
      421 ctgagagctc gtcgatgtag acgaagatcg attcggcgag caggctcaaa gtcttgccat
      481 ccagtccggc ctcgaggctc gccgccgcga tcctcctcca ggcgattcga gccccgatcc
      541 ggtaggccga ctggagtgaa tccagcgacc ggccctgtcg aagctcgccg cggccgagga
      601 tgacgtagac cttcctgctc gcccggcggt cggcatcggg gtcacggatc aggaccagga
      661 actgctcaag tgcttcgcca acaccccggt agacggcctt gccgaaggaa ccttcgagcg
      721 gcatcgcgta ttcggggatc aactggccaa tcgacccgag gatctcgtcg gcgatcccgg
      781 aaagctccgg ttcgatcaca tcggcgacct cggcatcgag gtctcgccag ggttcttgac
      841 gtgcagttct tataagaaga tttataacaa ttgcgtcaga taccttcgcg agaagggaag
      901 aaatactagg tatctccaca tatattgagg cgcatgacgc agcacaagat gcccctgctg
      961 gaccagatcg tgaacctgtt cgcagtggtc atcccgtttc tcgcgacgat cgcagcaatc
     1021 gttctcttct ggaaccagat cgtcacgccg cttgacctgg ccctgctggc cgggatgtac
     1081 ctgcttacga ccttcggaat cacgatcggg ttccatcggc tgttgaccca ccggtcgttt
     1141 cagaccttca agccggtcga atacttcctc gccatcgccg gttcgatggc ggttgagggt
     1201 ccggtcaaca cctgggtcgc cgaccaccgc aagcatcacg cccacaccga cgaggaaggc
     1261 gacccgcaca gcccccatgt cggtcacgac ggcggcatca agggggtgct gaagggtctc
     1321 tttcacgccc acatcggctg gactttcacc gaccacggca aggccgagca gcagaagtac
     1381 gcgaaggacc tctacgagga ccggggcatg aggttcatca gcgatttctt cgtgctctgg
     1441 gtcgtcctcg gccttctgat tccggccggt atcggctacc tggtgaccgg cacccttgcc
     1501 ggggcggcgg gtggcctgct ctggggtggg ctggtccggg tcttcttcgt ccaccacatc
     1561 accttttcga tcaactccgt ctgtcacttt ctcgggaccc gccggttcga catcgacgat
     1621 cactccacca atgtgttctg gcttgcgcta ccgtcaatgg gtgaggcctg gcatcacaac
     1681 catcacgcct tcccgcggtc ggccaaccac ggcctgaaga aatgggagct cgaccccagc
     1741 gcctgggtga tcaccgcgat ggaaaagacc gggctggccc gaaaggtggt ccgcatatcc
     1801 ccggaacgcc aggccgagaa actcgcccag cccgagaagc tcgcccagcc cgagaagctc
     1861 gcccagcccg agaagccacg gacccct
//
LOCUS       DAOTIP010000443         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_72669_length_1886_cov_5.140907, whole genome shotgun sequence.
ACCESSION   DAOTIP010000443 DAOTIP010000000
VERSION     DAOTIP010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72669_length_1886_cov_5.140907"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="P5138_08445"
     CDS             <1..417
                     /locus_tag="P5138_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV60646.1"
                     /translation="DFPGVDPSLPLYVFVFLVALGIDYNIFLMARVREETAVHGTREG
                     MLRGLAVTGGVITSAGIVLAGTFSVLAVLPLVFLAEMGFTVAFGVLLDTFLVRSILVP
                     ALTLDIGPKVWWPSALARQEESAGTGRPAEPDTAAG"
     gene            482..907
                     /locus_tag="P5138_08450"
     CDS             482..907
                     /locus_tag="P5138_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HRV60647.1"
                     /translation="MAARGSAEWKGTLREGGGTFTAGDSIGGDYSFKSRFEDGPGANP
                     EQLIAGAHASCFSMALSLVLEEAGTPVESVKTDAKVTLRLLDEGPSITKIELSTVGRV
                     PGIDQAAFEEAAAQAKVGCPISKALAAVPEITLEATLES"
     gene            complement(935..>1886)
                     /locus_tag="P5138_08455"
     CDS             complement(935..>1886)
                     /locus_tag="P5138_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60648.1"
                     /translation="VGYHLGGGLSFYARQHGLACNMVRAMEVVTADGEVARIDRDNEP
                     DLFWALRGGGGLFATVTAIEFDLLPFAEVYGGASFWPVETAEAALSTWLDWTRSAPES
                     VTSTFRIVNLPPFESVPEPIRGKNLVVIDGAALDNGDGDAFTARLEGLGDPVISQWGP
                     MPAPAVSTMHGDPEDPVPAIGNSTTIDEVGDELVEAFVEAAGGDSGCSLIAAELRQLG
                     GALRRPDPEGGVANMIDGEFLAFGVGLAAEPGMKAKTEGDLDRLMEAVSPWEGGRQYY
                     NFAVGGATCHDCYPEDDIEALKAIRREYDPEELYLAPLDL"
BASE COUNT          291 a          650 c          634 g          311 t
ORIGIN      
        1 gatttccccg gggtagaccc ctcgctgccg ctctacgtct tcgtattcct ggtcgcgctg
       61 ggcatcgact acaacatttt cctgatggcc cgggtgcggg aggagaccgc cgtccacggc
      121 acccgggaag gcatgttgcg cggcctcgcc gtgaccggcg gggtgatcac ctcggccggg
      181 atcgtcctgg ccggcacctt ctcggtcctg gccgtgctgc cgctggtctt cctcgccgag
      241 atgggcttca cggtcgcctt cggcgtcctg ctcgacacct tcctcgtgcg ctcgatactc
      301 gtgccggctc tcaccctcga catcggcccg aaggtctggt ggccttccgc cctcgccagg
      361 caggaagaaa gcgccgggac ggggcggccg gccgagccgg acacggccgc cggttagtct
      421 gggcgggagc cgggaaccag tcagacgttt cacccggaca accgaccagc agaggagcaa
      481 catggcagcc agaggcagcg cggagtggaa gggcacgctc agggaaggcg gaggaacctt
      541 caccgccggc gattcgatcg gcggcgacta ttcgttcaag tcacgcttcg aggacggccc
      601 gggggccaac ccggagcagc tgatcgccgg tgctcacgca tcctgttttt cgatggccct
      661 ttcactcgtc ctcgaggagg ccggcacccc ggtcgaatcc gtgaagaccg acgccaaggt
      721 gaccctgcgc ctgcttgacg aggggccgag catcacgaag atcgagcttt cgaccgtggg
      781 ccgggtgcca ggaatcgatc aggcggcctt tgaggaagct gccgcccagg ccaaggtggg
      841 ttgcccgatc tcgaaggcgc tcgcagccgt tcccgagatc accctggaag ccaccctgga
      901 gagctgataa ccgcccccgg accagcgggt cggttcagag gtccagcggg gcgaggtaga
      961 gctcttcggg gtcgtattcg cggcgaatcg ccttgagggc ctcgatgtcg tcttccgggt
     1021 agcagtcgtg gcaggtggcc ccgccgaccg cgaagttgta gtactgccgg ccaccttccc
     1081 agggactgac cgcctccatc aggcggtcca ggtccccttc ggtcttggcc ttcattccgg
     1141 gctcggcggc gaggccgacc ccgaaggcga ggaactcccc gtcgatcatg ttggccacgc
     1201 cgccttccgg gtccgggcgg cgcagcgcgc ccccgagctg gcggagttcc gccgcgatca
     1261 gcgagcagcc ggaatcgccg ccggccgctt cgacgaaggc ttcgacaagc tcgtctccga
     1321 cctcgtcgat cgtcgtcgag ttgccgattg caggcaccgg gtcctcgggg tcaccgtgca
     1381 tcgtggacac ggccggcgct ggcatcggac cccactgtga gatcaccgga tcgccgagcc
     1441 cttccagccg ggcggtgaag gcgtcgccgt cgccgttgtc cagggccgcg ccgtcgatca
     1501 cgacgagatt cttgccccgg atcggctccg gcaccgactc gaagggcggc aggttcacga
     1561 tccggaaggt gctggtcacc gactcgggcg cgctgcgggt ccagtcgagc caggtcgaaa
     1621 gggcggcctc cgcggtctcg accggccaga aactcgcgcc gccatagacc tcggcgaagg
     1681 gcagcaggtc gaattcgatc gcggtcactg tggcaaagag gcctccgccg ccccgcaggg
     1741 cccagaaaag gtcgggttcg ttgtcccggt cgatccgtgc cacttcgcca tccgcggtca
     1801 ccacctccat ggcccgcacc atgttgcagg cgagtccgtg ctgcctcgcg tagaaactga
     1861 gtccgccccc gaggtggtag ccgacc
//
LOCUS       DAOTIP010000444         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_72821_length_1883_cov_3.338074, whole genome shotgun sequence.
ACCESSION   DAOTIP010000444 DAOTIP010000000
VERSION     DAOTIP010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72821_length_1883_cov_3.338074"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /gene="pheT"
                     /locus_tag="P5138_08460"
     CDS             complement(<1..1867)
                     /gene="pheT"
                     /locus_tag="P5138_08460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRV60649.1"
                     /translation="MEIKQAKLRGVESNGMICSDRELELGEDHDGILVLNDGPAPGTS
                     ATEVLPLAEPVLELEVTPNRTDCFGIYGVAREAHAISAAPLSPAPWNGGPIGPGVDSE
                     AGDVERLDDAAGEVKTADRVRIMVRDEELCPRFTARGYTDVKVGPSPVWLKARLIAAG
                     MRPISNVVDITNYVMWLTGQPMHAYDLDLVPEGELVIRRAENGEKVAALDGDEYELTD
                     QMVAVADRNGLAGIGGMMGGSVSEVSASTTTVLMEAANWNGPNILRTSRDLGLRSEAS
                     SRFEKQLHPDLTLRAQAVAARLMVGICDATPMTGLVDVAAPPPSSEPIRLRAGRVERI
                     LGLAIDADRQAEALEMLGFGVEREGDDLLVKVPADRHFDVTREIDLVEEVGRISDLDR
                     NLPATLPSGAGRPGGLSRQQALQRRAEDSLRESGFDEMVGWSFTDPAEADRLRLDPED
                     PRAGAVRLANPLSEDQSVMRTTLLGSVLGTARRNLSRGADRVALFECGRVYLPSGAFG
                     PGPLGGDFPGNTRPPFNEPQHLCALAVGSVDPSSWRTEASGSDFFALKGVLEHLAGGL
                     GTAVTVERIPGAPTQPFLHPGKSGVVRAGDRPIGWIGEIHPLVAAEYDLGSAVAFE"
BASE COUNT          288 a          671 c          620 g          304 t
ORIGIN      
        1 tctcgaaagc gacggccgag ccgaggtcgt attcggccgc caccagcggg tgaatttcgc
       61 cgatccagcc gatcggccgg tctccggccc gcaccacacc ggacttgccc ggatggagga
      121 agggctgggt cggcgcgccg gggatccgtt ccacggtcac cgccgttccg aggccgccgg
      181 caagatgttc caggacgccc ttcagcgcga agaagtccga ccctgaagct tcggtccgcc
      241 aactcgacgg gtcgacggac ccgaccgcca gggcacagag gtgctgcggc tcgttgaatg
      301 gcggccgcgt attgccgggg aagtcccctc cgagtggtcc cggtccgaac gctcccgagg
      361 gcaggtagac ccggccgcat tcgaagagcg cgacccggtc ggcaccccgc gagaggttcc
      421 tccgggcagt tccgagcacg gacccgagca gggtcgtccg catcaccgac tgatcctcgg
      481 agagcggatt ggccagcctg acggcgccgg cccgcggatc ttcagggtcg agccgcagcc
      541 ggtccgcttc tgccgggtcg gtgaagctcc agccgaccat ctcgtcgaag cccgattccc
      601 tgagtgaatc ctcggcccgg cgctggagtg cctgctggcg gctgaggccg cccggccgcc
      661 ccgccccgga aggcagggtc gcgggcaggt tgcggtcgag atcgctgatc cgtccgactt
      721 cctcgaccag gtcgatctcc cgggtcacgt cgaagtgacg gtcggccgga accttgacca
      781 gcaggtcatc gccctcacgt tcgactccga agccgagcat ctccagcgct tcggcctgcc
      841 ggtccgcgtc gatcgccagt ccgaggatcc gctcgaccct ccccgcccgc agccggatcg
      901 gctccgacga aggcggcgga gcggcgacgt cgaccagccc cgtcatcgga gtcgcatcac
      961 aaatccccac catcagcctt gccgcgaccg cctgggcccg cagggtgagg tcggggtgaa
     1021 gctgcttctc gaagcgggac gaagcttcgg agcggagccc gagatcgcgc gacgtcctga
     1081 ggatgttcgg cccgttccag ttggcggcct ccatgagcac ggtggtggtc gaagccgaca
     1141 cttccgaaac cgaaccgccc atcatgccgc cgatcccggc cagtccgttg cggtcggcga
     1201 cggcgaccat ctggtcggtc aactcgtact cgtcgccgtc gagcgcggcc accttttcgc
     1261 cgttttcggc ccttctgatg accagttcac cctcgggcac gaggtccagg tcgtaggcgt
     1321 gcatgggctg gccggtcaac cacatcacat agttggtgat gtcgaccacg ttcgagatcg
     1381 gccgcatgcc ggccgcgatc agccgtgcct tcagccaaac gggcgagggg ccgaccttga
     1441 cgtcggtgta gccccgggcg gtgaaacggg ggcagagctc ctcgtcgcgg accatgatcc
     1501 gtacccggtc ggcggtcttc acctcaccgg cggcatcgtc cagcctctcc acgtctccgg
     1561 cctcggaatc gactccgggg ccgatcgggc cgccgttcca cggggccggc gagagaggcg
     1621 ccgcgctgat cgcatgggct tcgcgcgcta ccccgtagat gccgaagcag tcggtccggt
     1681 tgggggttac ctccagctcg agaaccggct cggccagcgg caacacttcg gtggcggaag
     1741 tcccgggcgc gggaccgtcg ttcaggacca gaattccgtc gtgatcctcg cctagctcga
     1801 gttcccggtc ggagcagatc atcccgttcg actcgacccc gcgcagtttc gcctgcttga
     1861 tctccattcc gcccggcatc acc
//
LOCUS       DAOTIP010000445         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_72863_length_1882_cov_4.289546, whole genome shotgun sequence.
ACCESSION   DAOTIP010000445 DAOTIP010000000
VERSION     DAOTIP010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72863_length_1882_cov_4.289546"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="P5138_08465"
     CDS             complement(<1..234)
                     /locus_tag="P5138_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60650.1"
                     /translation="MKKILITACSCLLMLAGTTSSGAALPQTDLFQDLNPGPSGSDVE
                     AFLPIGDQLWTSANSGQTGYEPWIVGKSKLNLVT"
     gene            complement(231..>1882)
                     /locus_tag="P5138_08470"
     CDS             complement(231..>1882)
                     /locus_tag="P5138_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60651.1"
                     /translation="LVMLLLSVTSASGAMVPRFALDVMPGPSGSYPFGMTEVNGTLLF
                     SADSQLGNELYGTSDGRSARLIKDLMPGEEDGDPGDFVRFGDFVYFLGYSPTAGYELW
                     RSDGQSATLFADINPGPDESYVNNLTVVGNTLFFTASTPAAGDELWKTDGTTTTMVQD
                     LYPGPGDSDPEYLTPFNGELYLTAESPATGPELYRSDGNTVSLVTDIEPGASGSSPSG
                     LTVFGSHIYFAAYKTGPSRELFRSDGVTTSLFKDVNPGAGYGSPEFLVASGGRLFFSA
                     TDGTHGSEPWSTDGTNTAIVADIAPGMDNSEVQEVAPFGNGRVIFNAEDPTHGEEPWT
                     ADATGANLVRDIRPGEEDSTPDEFYLLDGDIYFRANDGVHGRELWSTGGTGANLVKDI
                     LPGTDGSSPRNFFPYRGQTWFVADDGIHGREFWKLIPPDVSVTVKAPGRLRFRPSGRA
                     DLRITCPAGEATGPCSGKFALATRSKVNFRGKRRVVVLARGGFKAAAGKTDTIIIRLS
                     SAQRRLLHRVRAARNLKLTAEAKDKAGNKARITIRIKAVLP"
BASE COUNT          317 a          595 c          621 g          349 t
ORIGIN      
        1 ggtgacaagg ttgagctttg acttgccgac gatccagggc tcatatccgg tctgccccga
       61 attcgccgag gtccacagct ggtccccgat cggcaggaag gcttcaacgt cggaaccgct
      121 gggacccggg ttcaggtcct ggaagaggtc ggtctggggg agggcagccc ccgacgaagt
      181 ggtcccggcc agcatcaaca ggcagctgca tgcggtgatc aggatctttt tcacggcagc
      241 accgccttga tccggatcgt gatcctggcc ttgttaccgg ccttgtcctt cgcctctgcc
      301 gtcagtttca ggttcctggc ggcccggacc cggtggagca gcctcctctg ggcactgctc
      361 agccggatga tgatcgtgtc tgtcttgccg gccgcggcct tgaagcctcc gcgggccagg
      421 acgacgaccc tgcgctttcc cctgaagttg accttcgacc tggtcgccag agcgaacttg
      481 ccggagcagg gcccggtcgc ttcgccggcc gggcaggtga tccggaggtc ggccctgccg
      541 ctcggacgga aacgcagccg gcccggggcc ttgacggtga cggaaacgtc gggaggaatc
      601 agcttccaga actcccggcc gtggatgccg tcgtcggcca cgaaccaggt ctgcccccga
      661 taggggaaga agtttcgcgg cgaggatccg tcggtgcccg gaaggatgtc cttgaccagg
      721 ttcgctccgg ttccgccggt cgaccagagt tcccggccgt gtacgccgtc gttggcccgg
      781 aaatagatgt ccccgtccag caggtagaac tcgtccgggg tcgagtcttc ctctcccggc
      841 ctgatgtccc gaacgaggtt ggccccggtg gcgtcggctg tccacggttc ctccccgtga
      901 gtgggatcct ccgcgttgaa gatcacccgg ccgttgccga acggtgccac ttcctggacc
      961 tccgagttgt ccattccagg agcgatgtcg gcgactatcg ctgtattggt gccgtcggtg
     1021 ctccagggct cagatccgtg agtcccgtcc gtggccgaaa agaagagccg gccgcccgat
     1081 gccaccagga actcggggct gccatatccg gcgcccggat tcacgtcctt gaagaggctg
     1141 gtcgtgacgc cgtcactccg gaacagttcc cggctgggtc cggtcttgta agccgcgaag
     1201 tagatgtggg agccaaagac ggtaaggccg ctaggggagc tacccgaggc cccgggttcg
     1261 atgtcggtaa ccagactgac cgtgttgcca tcggagcggt agagctccgg gccggtggcc
     1321 ggcgattccg cggtcagata cagctccccg ttgaagggcg tcaggtactc gggatccgaa
     1381 tcacccggtc ccggatacag atcctggacc atggtcgtgg tcgttccgtc ggtcttccag
     1441 agttcgtccc ctgcggcggg tgtcgaggcc gtgaagaaca gggtgttgcc gaccacggtc
     1501 aggttgttga cgtatgactc gtccgggccc ggattgatgt cggcgaaaag tgtcgcgctc
     1561 tggccgtccg agcgccagag ctcatagccc gctgtgggtg aatagccgag gaaatagacg
     1621 aagtcaccaa agcggacgaa atctcccgga tccccgtcct cctcgcccgg catcaggtcc
     1681 ttgatcagtc tggccgagcg cccgtccgag gtgccataga gctcattgcc caattgcgag
     1741 tccgcggaga agagcagcgt tccgttcacc tccgtcatgc cgaacgggta agagccactg
     1801 ggaccgggca tcacgtcaag ggcgaagcgc gggaccatgg ccccgctcgc cgaagtcacg
     1861 gacaggagca acatcacgag cg
//
LOCUS       DAOTIP010000446         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_73127_length_1877_cov_4.451153, whole genome shotgun sequence.
ACCESSION   DAOTIP010000446 DAOTIP010000000
VERSION     DAOTIP010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73127_length_1877_cov_4.451153"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="P5138_08475"
     CDS             <1..1127
                     /locus_tag="P5138_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60652.1"
                     /translation="AASSSHSNYELVRNVVNSSNYVSQFNLNAYPVESADVLHMGPGA
                     FDAALNTVNIGKFNLGFASIGICEHAFWEAITHADNRVLYGMKVTDFPQVRQAFTDAF
                     ARAVAMKLFAWRAVDYFRAASPDDRRYLLFNPLTKMKVTTEGEKVIDLLWDVIAAKGF
                     ERDMWFEMAARDIRALPKLEGTVHVNMALVLKFMASYLFNPDPDLQAPDRMQEPGDDD
                     FLFRQGPARGLGKIRFSPWEKSYEPFAGVPNVARFMELAESFKSFLVECGPDEDQQKD
                     LDFLLTLGEIFTLIPYGQLICEQAALIGLPEDTLDQIFDVMIRDFSAYAAELHGKTST
                     TEAQAEWARSAITRPVVDQDRFDSVWEKTRALAGGYEMNP"
     gene            1138..1563
                     /locus_tag="P5138_08480"
     CDS             1138..1563
                     /locus_tag="P5138_08480"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRV60653.1"
                     /translation="MQRMRFDKDFDLPPDQVFSFFAEHENLGGVLGAEVTRLSDGTDG
                     HRNGVGSSRRLKPPGPVPAFEETVTEFVPDEKIVYKITKGTPLNHHLGTVTFAPTESG
                     THMEWNIEIGAAFPGLDFIISKVLIRNIGGGLDRMSIPA"
     gene            complement(1568..>1877)
                     /locus_tag="P5138_08485"
     CDS             complement(1568..>1877)
                     /locus_tag="P5138_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60654.1"
                     /translation="IRMVPVAISTIGTGLKRSSVLFMGWFGPRGLASIILALVVIEEE
                     PELPALATVLAAMTFTVLVSVFAHGLTAEPLINLYSRVIRSDGAAAESEEAMELQIRK
                     "
BASE COUNT          359 a          622 c          568 g          328 t
ORIGIN      
        1 tcgccgccag ctccagccac agcaactacg agctggtccg caacgtggtc aactcgtcga
       61 actacgtctc gcagttcaac ctcaacgcct atccggtcga gtcggcggat gtactgcaca
      121 tgggacccgg cgccttcgac gccgccctga acacggtcaa catcggcaag ttcaacctag
      181 gtttcgcctc gatcgggatc tgcgagcacg ccttctggga agcgatcacc catgccgaca
      241 accgcgtgct ctacggcatg aaggtgaccg acttccccca ggtccggcag gccttcaccg
      301 acgccttcgc ccgggcggtc gcgatgaagc tcttcgcctg gcgggcggtc gactacttcc
      361 gcgccgcctc gccggatgac cggcgctacc tgctcttcaa cccgctgacc aagatgaagg
      421 tgacgaccga gggcgaaaag gtgatcgacc tgctctggga cgtgatcgcg gccaagggct
      481 tcgaacggga catgtggttc gagatggcag cccgggacat tcgagccctg ccgaaactgg
      541 agggcacggt ccacgtcaac atggccctgg tcctcaagtt catggcctcg tacctgttca
      601 acccggaccc agacctgcaa gcgcccgacc ggatgcagga gccgggcgac gacgatttcc
      661 tcttccgaca gggccctgcc cgcggtctcg ggaagatccg cttcagcccc tgggagaaat
      721 cctatgagcc tttcgccggg gtgccgaacg tggcgaggtt catggagctg gccgagtcct
      781 tcaagagctt cctggtcgaa tgcggtcccg acgaggacca gcagaaggat ctcgacttcc
      841 tgctcaccct gggcgagatc ttcaccctga tcccctacgg ccagctgatc tgcgagcagg
      901 cggccctgat cgggctgccg gaggacacgc tcgaccagat cttcgacgtg atgatccgag
      961 acttctccgc ctacgccgcc gagctccacg gcaagacatc gaccacggag gcacaggccg
     1021 agtgggcgcg ttcggcgatc accaggccgg tggttgacca ggaccggttc gactcggtct
     1081 gggagaagac ccgcgctctg gccggcggct acgaaatgaa cccgtagaat cgcgctcatg
     1141 cagcggatgc gattcgacaa ggattttgac ctgccgcccg accaggtgtt cagcttcttc
     1201 gccgagcacg aaaacctggg gggcgttctc ggagcagagg tgaccagact gagcgacggc
     1261 actgacggcc accgcaacgg ggtcggctct tcacgtcgcc tgaagccgcc cggcccggtt
     1321 cccgccttcg aggagacggt caccgagttc gtaccggacg agaagatcgt ctacaagatc
     1381 accaagggaa ccccgctgaa ccatcatctc ggcacggtca ccttcgcgcc gaccgaatcc
     1441 gggactcaca tggagtggaa catcgagatc ggcgccgcct ttcccggtct cgacttcatc
     1501 atctccaagg ttctgatcag gaacatcgga ggcgggctcg accgcatgtc gattcccgcc
     1561 tgaggtctca tttcctgatc tggagttcca tcgcctcctc gctctcggcg gcggctccgt
     1621 ctgacctgat cacgcgagag tagaggttga tcagcggttc ggccgtcagt ccgtgggcga
     1681 agacgctgac cagaacggtg aaagtcattg ccgcgaggac ggtcgcaagg gcaggcagct
     1741 ccggttcctc ctcgatcacg accagggcaa ggatgatcga ggcgagtccc cgcggtccga
     1801 accaccccat gaagaggacc gagcttcgct tcagcccggt cccgatcgtg gatattgcga
     1861 ccgggaccat cctgatc
//
LOCUS       DAOTIP010000447         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_73277_length_1875_cov_2.716484, whole genome shotgun sequence.
ACCESSION   DAOTIP010000447 DAOTIP010000000
VERSION     DAOTIP010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73277_length_1875_cov_2.716484"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="P5138_08490"
     CDS             complement(<1..382)
                     /locus_tag="P5138_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRV60655.1"
                     /translation="MSQSPEERPDLTELRRRRSLTLDEARPDAVDRRHERGGRTAREN
                     LDDLVDAGSFVEYGRYAIAPQRARFETEDLIARTPADGLIAGTASVNGDLFGEAARCA
                     VLSYDYTVLAGTQGSRVAVILYQDE"
     gene            449..1156
                     /locus_tag="P5138_08495"
     CDS             449..1156
                     /locus_tag="P5138_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60656.1"
                     /translation="MGGIGSRIALGLAVGMAAFVACLGAAEALAPESGTVIASDPTCC
                     TYLPGPYAQDQGQAAYFDNTASTTYHDVQAKGNGPDGKPLFSMTSLAPGGSILRVNGT
                     EYLKPGSYPFYCTLHGAAMSGTLIINDAGTAVARPSVKVSIPAQKLRKVRKSGVKVKV
                     KALTASKGVFLFAKKGKVTLGSKRGLSLNAGQSKTLTLRLTKAGRKAIKKGKSAKIKV
                     SASVPFGKTSTASRKVK"
     gene            1161..>1440
                     /locus_tag="P5138_08500"
     CDS             1161..>1440
                     /locus_tag="P5138_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60657.1"
                     /translation="MTPRKLVLAVLALSVIFILPQSASALLPHSTTTRLANGAERITY
                     RVGPLTVTPGQNRIAFRPMTGVEKPDVDGWITRIKPDMVREDGSIPLSS"
     assembly_gap    1441..1450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1451..>1875
                     /locus_tag="P5138_08505"
     CDS             <1451..>1875
                     /locus_tag="P5138_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60658.1"
                     /translation="HHGVWINYSRTDATAGGPERFFATGEEKTITQFPPGYGYQYKKS
                     DVWILNHMIHNLTPEAMTLYATYTIDFIPADSPAADGIKPVTPIWMDVDNGSIYPVFD
                     VWRHSGDKDGKFTYPDEAKNPYPNGDEKNKWTVDRDGVLL"
BASE COUNT          367 a          602 c          589 g          307 t
ORIGIN      
        1 cctcatcctg gtagaggatg acggccacgc gggagccctg ggtgccggcc aggaccgtgt
       61 agtcgtagga cagaacggcg cagcgcgcag cttcaccaaa gagatcgccg ttgacgcttg
      121 ccgtgccggc gatgagtccg tcggccggtg tacgggcgat cagatcttcc gtctcgaagc
      181 gggcccgctg cggggcaatc gcgtatcggc cgtattcgac gaagctgccg gcgtcgacca
      241 ggtcgtcgag attctcgcgg gcggtacgcc cgcctcgctc gtggcggcga tccacggcgt
      301 caggccgcgc ctcgtccagg gtgagagagc ggcggcgtct gagctcggtc agatctggcc
      361 gttcttcagg gctctgcgac attcccgaaa actgtcacat cccggcaatt tctgcgcgca
      421 gagttgatat gcgtcatgat gtattccgat gggagggatt ggttccagaa ttgcattggg
      481 gctggcggtc ggtatggctg ccttcgtggc ctgcctgggc gccgccgagg cgctggctcc
      541 ggagagcggg accgtgatcg cctccgaccc gacctgctgt acctatttgc cgggacccta
      601 cgcgcaggac cagggtcagg ctgcgtactt cgacaacacc gcttcgacca cctaccacga
      661 cgttcaggcc aaggggaacg gcccggacgg caagccgctc ttctcgatga ccagcctggc
      721 gccgggcgga tcaatcctgc gggtgaacgg aactgaatac ctgaagccgg ggagttaccc
      781 gttctactgc acgcttcacg gagcggcgat gagcggaacc ctgatcatca acgacgccgg
      841 caccgcggtt gcccgtcctt cggtcaaggt ttcgatcccc gcccagaagc tccggaaggt
      901 ccgcaagtcc ggggtgaagg tgaaggtcaa ggcactcact gcaagcaagg gtgtgttcct
      961 gttcgcgaag aagggcaagg tgaccctcgg gtcaaagcgc ggtctgagcc tgaacgccgg
     1021 ccagtcgaag acgctcaccc tgcggctgac caaggcgggg cgcaaggcga tcaagaaggg
     1081 caagtccgcg aagatcaagg tcagcgcttc ggtccccttc ggaaagacct ccaccgccag
     1141 ccgaaaggtg aagtgatcgg ttgacccccc gcaagctcgt cctcgcagta ctcgctctct
     1201 cagtcatctt cattctgccc cagtctgcct cggcacttct gccgcacagc accaccacca
     1261 ggctcgcaaa cggagccgag cggatcacct accgggttgg cccgctgacc gtaaccccgg
     1321 gccagaacag aatcgccttc cggcccatga ccggggtcga gaagccggat gtggacggct
     1381 ggatcacacg gatcaagccg gacatggtca gggaggacgg ctcgatcccg ctttcgagca
     1441 nnnnnnnnnn caccacgggg tatggatcaa ttacagccgg accgacgcca ccgcgggcgg
     1501 gcccgaacgg ttcttcgcga ccggcgagga aaagacgatc acccagttcc ctccggggta
     1561 cggctaccag tacaagaagt ccgacgtgtg gatcctcaac cacatgatcc acaacctcac
     1621 gccggaagcg atgacgcttt acgcgaccta caccatcgac ttcattccgg ccgactcgcc
     1681 ggccgccgac ggcatcaagc cggtcacgcc gatctggatg gacgtggaca acggcagcat
     1741 ctacccggtc ttcgacgtct ggcgtcacag cggcgacaag gacggcaagt tcacctaccc
     1801 cgacgaggcg aagaacccgt acccgaacgg ggacgagaag aacaagtgga ccgttgatcg
     1861 tgacggcgtc ctgct
//
LOCUS       DAOTIP010000448         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_73494_length_1871_cov_3.144273, whole genome shotgun sequence.
ACCESSION   DAOTIP010000448 DAOTIP010000000
VERSION     DAOTIP010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73494_length_1871_cov_3.144273"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="P5138_08510"
     CDS             complement(<1..149)
                     /locus_tag="P5138_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60659.1"
                     /translation="MIEIQGVTKSFGEREVLGGIDLTLREHEAIALIGASGSGKSTLL
                     RCIDLL"
     gene            complement(167..877)
                     /locus_tag="P5138_08515"
     CDS             complement(167..877)
                     /locus_tag="P5138_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRV60660.1"
                     /translation="MVFTSSGWPDVRETFFSSEAFKTSFPDILDGFWLDVKLFLVVEV
                     IVLMLGLLVAVIRTSRSPALFPIRLLAAFYCDFFRGVPVILLVYLVGFGIPALALEGV
                     PTDPIILAGVALSLAYSSYVAEVYRAGIASVHPSQTDAGLATGLTRIQTLRFVVIPQA
                     VRRVRPPLLNDFISLQKDVALVSILGISEAFRIAQVEAASTFNYTPLIAAALLYLAVT
                     FPMARILDHITAKEQINS"
     gene            complement(1023..>1871)
                     /locus_tag="P5138_08520"
     CDS             complement(1023..>1871)
                     /locus_tag="P5138_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV60661.1"
                     /translation="LIAIFALALFVGCGSSDDSSDEGSSSSDGATTASSCAPGDLNTF
                     KDGVLTIGTDSPAYPPYFEDDDPTNGKGFESALGYAIAGELGFNDSQVEWTTVPFDAS
                     YRPGPKDFDFDLNQISITPDREKAVSFSVPYYTANQAVLVKKGSDLDGITSLDDLKDA
                     SIGVQVGTTSLTAVEDQIQPNSDPKIFNNSNDVVTAFKQDQVDAIVVDLPTAIYLRDA
                     ELSGTVVAGQFEQEGGDQWGALMTKDSDLKGCVDKAINTLKENGTLEEITNQWMADSA
                     KAPMLS"
BASE COUNT          355 a          563 c          626 g          327 t
ORIGIN      
        1 agcaggtcga tgcagcggag gagggtcgac ttgccggaac cggaggcgcc gatcagggcg
       61 atcgcctcgt gctcgcgcag ggtcagatcg atgccgccga gcacctcccg ctccccgaag
      121 gacttggtca ctccctggat ctcgatcacc ggtgtcctgg cggcactcat gagttgatct
      181 gctccttggc agtgatgtgg tcgaggatac gggccatcgg gaaggtgacc gcgaggtaga
      241 gcagcgcggc ggcgatcagt ggcgtgtagt tgaaggtcga ggccgcctca acctgggcga
      301 tgcggaacgc ttcgctgatg ccgaggatcg agaccagggc gacgtctttc tggagcgaaa
      361 tgaagtcatt gagcagtggc ggcctgaccc ggcggaccgc ctgcggaatc accacaaagc
      421 gcagggtctg gatgcgggtc agcccggtgg ccagccctgc atcggtctgg ctgggatgga
      481 ccgaggcgat cccggcccgg taaacctcgg ccacatatga gctgtaggcc agcgagaggg
      541 cgaccccggc aagaattatc ggatctgtgg gaaccccctc cagggcaagc gccgggatac
      601 cgaagccgac cagatagacc agcaggatta ccggcacccc gcgaaagaaa tcgcagtaga
      661 aagcggcgag caggcgaatc ggaaagaggg ccggactgcg gctggtgcgg atcaccgcca
      721 ccagcaggcc gagcatcagc acgatcacct ctacgaccag gaacagcttc acgtcaagcc
      781 agaagccgtc gagaatgtcg ggaaaggacg tcttgaaggc ctcggaggag aagaaggtct
      841 cacggacgtc cggccagccg ctgctggtga acaccacgag cgccagcccg cccaggatta
      901 caagtgatga aaaagcggcg atcgcctggc cgcgccgcgc cttggccttt ctggcctttt
      961 cgcgggcggc ccggcgaccg ccggtaccgc tttgaactgg agcttccgtg atcagaccct
     1021 gatcagctca gcatcggagc cttggccgag tccgccatcc actggttcgt gatctcttcc
     1081 agggtgccgt tctccttgag cgtgttgatc gccttgtcga cgcacccctt gagatcggag
     1141 tccttggtca tcagcgcgcc ccactggtcg ccgccctcct gctcgaactg gccggctaca
     1201 acggtgccgg agagttcggc atcccgcagg tagatggcgg tcggcagatc gaccacgatc
     1261 gcgtcgacct ggtcctgctt gaaggcggtc accacatcat tcgagttgtt gaagatcttc
     1321 ggatcgctgt tgggctggat ctgatcctcg accgcggtga ggctggtcgt gccgacctgg
     1381 acgccgatcg aggcgtcctt caggtcatcg agactggtga tcccgtcgag atcgctgccc
     1441 ttcttgacca gtaccgcctg gttcgcggtg tagtacggaa cagagaacga aaccgccttc
     1501 tcgcggtccg gggtgatcga gatctggttc aggtcgaagt cgaaatcctt cggacccggg
     1561 cggtaggaag cgtcgaaggg aaccgtggtc cattcgacct gggagtcgtt gaaaccgagc
     1621 tcaccggcga tcgcgtagcc gagggcgctc tcgaagccct ttccgttggt cgggtcgtcg
     1681 tcctcgaagt agggcgggta ggccggcgag tcggtgccga tcgtcagcac tccgtccttg
     1741 aaggtgttga gatcccccgg ggcgcagctg gacgccgtgg tcgcgccgtc ggacgacgac
     1801 gaaccttcgt cggatgaatc gtcactcgat ccgcagccca cgaaaagggc cagggcgaag
     1861 atggcgatca g
//
LOCUS       DAOTIP010000449         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_73659_length_1868_cov_3.796470, whole genome shotgun sequence.
ACCESSION   DAOTIP010000449 DAOTIP010000000
VERSION     DAOTIP010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73659_length_1868_cov_3.796470"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..962)
                     /gene="rpsD"
                     /locus_tag="P5138_08525"
     CDS             complement(342..962)
                     /gene="rpsD"
                     /locus_tag="P5138_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRV60662.1"
                     /translation="MGRYTGPKEKLSRREGVDLGLKGERALGGKSAMERRPYPPGQHG
                     RGRRRASEFSVRMRAKQRAKAMYGLREKQFAKLFDRANREEGMAGENLMRHLESRLDN
                     VVYRLGLAATRAQARQFVVHQHIQVNGKRLDRPSYEVSAGDDIAVKPGSAIEPMARQS
                     TESAGPTPAWLLADHDGLKGTVNRLPDRNDIAAPIEERLIVEHYSR"
     gene            1069..1590
                     /locus_tag="P5138_08530"
     CDS             1069..1590
                     /locus_tag="P5138_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRV60663.1"
                     /translation="MESLKGRLLVAAPSLFDFFRRTVVLVVEHTEQGAFGVILNRRAD
                     TEVAELVPDLGPLVEQGAPLWVGGPVGPDSIVVLGDFNNPGESAGVVEGSIGVVDPDG
                     DAEFSRARVFVGHAGWGPGQLEGEVERDSWIISDFDPDDVFSEGDLWADVVRRPGGDY
                     ALLATMPDDPTLN"
     gene            1613..>1868
                     /locus_tag="P5138_08535"
     CDS             1613..>1868
                     /locus_tag="P5138_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV60664.1"
                     /translation="MTFQSPERSPLSRNDLDPDPIAQFRIWFDAAGEVMPLPEAMTVA
                     TVDGEGRPDARMVLLKGAGDDGFRFFTNYEGVKAGQLDESG"
BASE COUNT          277 a          563 c          669 g          359 t
ORIGIN      
        1 cgcttgcctc ctcggcagcg cacggctgga ggcccctgtg cacgacccgc tgaaggtggg
       61 ttctggcatg cctcggggaa gcctcgctag ccgtggggtc ggtcatgcgc ccccgggcaa
      121 ttgagcaggg ttcggtggtg tgcttccgaa acctctggat ggggagcgcg aagcgcgacg
      181 gtttcggagg tacaccaccg ggcgctgcag caatgcctga ggtgagccgg ttctttgaat
      241 cgctgccgcc ggccccgcgg ctacaccacc gggcacgggg atgcaagaga aagggccccg
      301 gttttccgga gccctttgaa gttctgcttt tctcgcggtg tctagcgcga gtagtgctcg
      361 acgatcaggc gctcttcgat cggagcggcg atgtcgttgc ggtcgggcag acggttgacc
      421 gtgcccttga ggccgtcatg gtcggccagc agccaggccg gggtcggacc tgccgactcg
      481 gtgctctggc gggccatcgg ctcgatcgcc gaaccgggct tgacggcgat gtcgtcgccg
      541 gccgaaacct cgtaggaggg acggtcgaga cgcttgccgt tgacctggat gtgctggtgg
      601 acgacgaact ggcgggcctg ggcgcgggtc gcggcgaggc ctaggcggta gacgacgttg
      661 tccaggcgcg actcgaggtg gcgcatcagg ttctcgccgg ccatgccctc ttcacggttg
      721 gcccggtcga agagcttcgc gaactgcttc tcgcgcaggc cgtacattgc cttggcgcgc
      781 tgcttggcac gcatacggac cgagaactcg gaagcacggc gccggccacg gccatgctgg
      841 ccgggcgggt aggggcggcg ctccatggcg ctcttgccgc caagggcacg ttcacccttg
      901 agtccgagat ctacgccttc acggcgggaa agcttttctt ttggtcctgt gtatctgccc
      961 atagtgcggc gaatgagcct acaggatcgg gcgatttccc gctttgagcg agttgttcgc
     1021 ggcaagcggt cgttcgcgag acgccgaacc gggtcctata gtcggagcat ggagtcgctc
     1081 aagggacgtt tgctggtagc cgcaccgagc cttttcgatt tctttcgtcg gaccgtggtc
     1141 ctggtcgtcg agcacacgga gcagggagcg ttcggggtga ttctgaaccg gcgggccgac
     1201 accgaagtcg cggagctcgt tcccgacctc gggcctctgg tcgagcaggg cgctcccctc
     1261 tgggtcggcg gtccggtcgg ccccgattcg atcgtcgtcc tcggtgattt caacaatccc
     1321 ggcgagtcgg ccggggtggt ggagggcagc atcggggtgg tcgatcccga cggcgacgcc
     1381 gagttctcgc gggcgcgggt gttcgtcggc catgccggct ggggccccgg gcaactcgaa
     1441 ggggaggtcg agcgggattc ctggatcatc tcggacttcg atccggatga tgtcttctcc
     1501 gaaggtgatc tctgggcaga tgtggtccgg cggccgggag gcgactacgc gctgctggcc
     1561 acgatgccgg atgacccgac tctgaactga accggccaaa taggcttggg ccgtgacttt
     1621 ccaatcacct gagcgctcac cgctttcaag gaacgatctc gacccggacc cgatcgccca
     1681 gttcaggatc tggttcgatg cggccggcga ggtgatgccg cttcccgagg cgatgaccgt
     1741 cgccacggtc gacggggagg gccggcccga tgcccggatg gttctgctga aaggcgcggg
     1801 tgacgacggt tttcgtttct tcaccaacta tgagggtgtg aaggccggcc agctcgacga
     1861 atccggac
//
LOCUS       DAOTIP010000450         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_73681_length_1868_cov_2.831219, whole genome shotgun sequence.
ACCESSION   DAOTIP010000450 DAOTIP010000000
VERSION     DAOTIP010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73681_length_1868_cov_2.831219"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1868)
                     /locus_tag="P5138_08540"
     CDS             complement(<1..>1868)
                     /locus_tag="P5138_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRV60665.1"
                     /translation="RLGSPNLRLAIYDGDTPRDERPAIRRQSNLVLTNPDMLHVGIMP
                     HHRGWGDFLANLDWVVVDEAHTYRGVFGSHVANVLRRLRRLARAYGREPRFVMASATI
                     ANPAELAERLTGAPARLIDEDGAPSAGREIRIWNPPLVNPAIGARRSALAEAADLLAD
                     LVSHEVRTICFLKSRKGIELIRKFAADRLTERGRPDLAGRIAPYRAGYTPAQRREIEA
                     KLVSGELLGVASTDALELGIDIGGLDAAICVTFPGTVAGLKQMWGRAGRRERGLAVYV
                     AGPDGLDQYFCRHPDEFLERPVEAAILDHLSPEIRDQHLVAAAFELPLTAEDEEFFGP
                     GLEDAGQALVLRGELRKAGKGLVPRRAGFAASDVSLRSSSVGDIAVIDRESGEVIGTV
                     ETGRAFTTIHPGAVYMHLGTSWEVEMLDPVEGQAIVHRFDDEWYTRPKVETEIFIDRT
                     LETREVCGVKLSFGHLSVTDQVMAYQRILSADGEAFDLVDLELPEQEFVTRGLWYEVP
                     EDFISGMALNEMLGALHATEHGQIAVLPLIAMCDRWDIGGLSTNLHMQTGTPTIFIYD
                     GHPGGIGLTRRAFDLFPRLVGDALRLIGECPCDSGCPSCVQSPKCGNLNEPLFKAG"
BASE COUNT          304 a          615 c          643 g          306 t
ORIGIN      
        1 tcccgccttg aacagtggct cgttcaggtt cccgcatttg gggctctgaa cgcaagaggg
       61 gcaaccggaa tcgcagggac attcgccgat cagtcgcagg gcgtcaccga ccagccgggg
      121 gaaaaggtcg aaggcccgtc tggtcaggcc gatgccgccg ggatgtccgt cgtagatgaa
      181 aatcgtcggg gtgccggtct gcatgtgcag gttggtcgaa aggccgccga tgtcccagcg
      241 gtcacacatc gcgatcagcg gcagcaccgc gatctgtccg tgttcggtcg cgtggagtgc
      301 gccgagcatc tcgttcaggg ccatgcccga aatgaagtcc tccggcacct cgtaccagag
      361 cccgcgggtc acgaactcct gttccggcag ttcgaggtcg accaggtcga aggcctctcc
      421 gtcggcgctg aggatccgct ggtaggccat cacctgatcg gtgaccgaga gatgaccgaa
      481 gctgagtttc accccgcaga cctccctcgt ttcgagagtg cgatcgatga agatctcggt
      541 ctctaccttc ggccgggtgt accactcgtc atcgaaacgg tggacgatcg cctgtccttc
      601 caccgggtcc agcatctcga cctcccagga ggtgccgaga tgcatgtata cggcaccggg
      661 atgaatggtg gtgaaggccc ggccggtctc gaccgtgccg atgacctcgc ccgactcgcg
      721 gtcgatcacc gcgatgtcac cgaccgagga ggaacggaga gaaacgtccg aggcggcgaa
      781 gccggctcgt cgcggtacca gccccttgcc ggccttgcgc agttcccccc gcaggaccag
      841 tgcctgcccg gcgtcctcga ggcccggacc gaagaactcc tcgtcctcgg ctgtcagggg
      901 gagctcgaag gcagcggcga cgaggtgctg gtcgcggatc tccggggaga ggtggtcgag
      961 gatcgcggct tctaccgggc gctcaaggaa ctcgtcggga tgacggcaga agtactggtc
     1021 tagtccgtct ggcccggcga cgtaaacggc aaggccccgc tcgcggcggc cggcacggcc
     1081 ccacatctgc ttcaggccgg cgaccgtgcc ggggaaggtc acgcagattg ccgcgtcgag
     1141 tccgccgatg tcgattccca gctcgagagc gtcggtcgaa gccacgccga gcagctcgcc
     1201 ggagaccagc ttcgcctcga tttcccgtcg ctgggccggg gtgtaaccgg cccggtaggg
     1261 agcgatccgc ccggccaggt cgggacggcc cctctcggtc aggcggtcgg cggcgaactt
     1321 gcggatcagc tcgattccct tgcgactctt gagaaagcag atcgtccgca cctcatggga
     1381 aaccaggtcg gccagcaggt cggccgcctc ggccagggca gaacgcctcg ctccgatcgc
     1441 cgggttgacc agcggcgggt tccagatccg gatttcccgc ccggcgctcg gggccccgtc
     1501 ctcgtcgatc agccgggccg gcgctccggt gagccgttcg gccagctcgg ccggattggc
     1561 gatcgtggcc gaagccatca cgaagcgcgg ctcccgtccg taggcccggg ccagccgcct
     1621 gagccgtcgc agcacgttgg cgacatggga tccgaagaca ccgcggtagg tgtgggcctc
     1681 gtcaacgacc acccagtcga ggttggccag gaaatcgccc cagccgcggt ggtggggcat
     1741 gatcccgaca tgcagcatgt ccgggttggt caggacgagg ttgctctgcc tgcggatcgc
     1801 cggccgctcg tcccgcgggg tgtcgccgtc gtagatcgcc aggcgcaggt tcggtgagcc
     1861 gagccggg
//
LOCUS       DAOTIP010000451         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_73821_length_1865_cov_3.219890, whole genome shotgun sequence.
ACCESSION   DAOTIP010000451 DAOTIP010000000
VERSION     DAOTIP010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73821_length_1865_cov_3.219890"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="P5138_08545"
     CDS             <1..232
                     /locus_tag="P5138_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV60666.1"
                     /translation="GPGVPVADRERIFNRFARLDQARSSASGGSGLGLAIVAAIAGAH
                     GGVARCTDSPLGGARFEIEIPVTGSGGVPPSR"
     gene            242..1411
                     /locus_tag="P5138_08550"
     CDS             242..1411
                     /locus_tag="P5138_08550"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRV60667.1"
                     /translation="MRTSADRPLNVQPATVRDYRELARRRLPRQLFDYADGGAYEETT
                     MRANESDLEKVLLRQRVMRDVSVREQSIELLGQELSMPVILGPVGLAGMFAPRAEVQA
                     ARAAEAAGVPFTESTVSICSIEEVAEATSVPPWFQLYVMRDRAYAEKLMARAREVGSP
                     VLMLTIDLAVVGARHRDTRNAMVGNPSTWAKVRRGLDIASHPGWVKSVALGGKPLTFG
                     NLAEVVPGARTPEDFKGWVDSQFDPSVTWDDIAWVREHWKGKLVVKGVLDSEDARRAV
                     DAGVDGIVVSNHGGRQLDAVPSTASALPGIAEAVGSQTTVIADGGVRNGLDMVKMLAL
                     GARAVIVGRAWAWAVAAEGQAGVENMLKVLRADADVALALTGSNSVTELGPDSLA"
     gene            complement(1380..>1865)
                     /locus_tag="P5138_08555"
     CDS             complement(1380..>1865)
                     /locus_tag="P5138_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60668.1"
                     /translation="TAKVLRNGRKVGSKKVATDGIIEVSVAAPRSKKARKTARYSLKV
                     GSSSSKRMRASSAIRVLFRKPLPAAEQVRARLSGIRRKGSLTLKSVPLCGGSPSNRKV
                     RHNRRGIFTVRLGFGPATQIFEIRKRGRRVYLPVVMPAARFVLADRASRLRPANPGRV
                     R"
BASE COUNT          288 a          602 c          654 g          321 t
ORIGIN      
        1 cggaccggga gtgccggtgg ccgaccggga acggatcttc aatcgtttcg cccgactcga
       61 tcaggcgaga agcagcgcct ccgggggttc cggattgggt ctcgcgatcg ttgcggccat
      121 agccggggcc catggcgggg tcgcacgctg cacggattcg cccctcggcg gcgcccggtt
      181 cgagatagag attcccgtta ccgggtcggg tggtgttccg ccgagccggt agtttgcggc
      241 catgcgaact tccgcggatc gccccctgaa cgtgcagccc gcgaccgtgc gggactaccg
      301 cgaactggcg cggcgcagat tgccgcgcca gctcttcgat tacgccgacg gcggtgccta
      361 cgaagagacg acgatgaggg cgaacgagtc cgacctggag aaggtccttc tcaggcagcg
      421 ggtcatgcgg gatgtgtcgg tccgcgaaca gtcgatcgag ttgctgggcc aggaactctc
      481 gatgcccgtg atcctcgggc cggtgggcct ggccggcatg ttcgcgcccc gggcagaggt
      541 ccaggcggcg agggcagcgg aggccgccgg ggttcccttc accgagtcca ccgtttcgat
      601 ctgttcgatc gaggaggttg cggaagcgac cagcgtcccg ccctggttcc agctctacgt
      661 gatgcgggac cgcgcctatg ccgagaagct gatggccagg gcccgtgaag tcggctcgcc
      721 ggtgttgatg ctcaccatcg acctcgccgt ggtcggcgcc cgacaccggg acacccgcaa
      781 cgcaatggtc ggcaatccct ccacctgggc aaaggtccgc cggggactgg acatcgcctc
      841 ccacccgggc tgggtcaaat cggtcgcgct cgggggcaag ccgctcacct tcggcaatct
      901 cgccgaagtg gtgcccggtg cacggactcc ggaagacttc aagggatggg tggactccca
      961 gttcgaccct tcggttacct gggacgacat cgcctgggtg cgggaacact ggaaagggaa
     1021 gctggtcgtc aagggcgtgc tcgactccga ggacgcgcgc cgcgcggtcg atgccggggt
     1081 cgacgggatc gtcgtctcca accacggtgg ccgtcagctc gatgcggtgc cctccactgc
     1141 atcggccctg cccgggatcg ccgaggcggt cggcagccag accaccgtga ttgccgacgg
     1201 cggcgttcgc aacgggctcg acatggtcaa gatgctcgcg ctcggtgcca gggcggtgat
     1261 agtcggccgg gcctgggcct gggcggtcgc cgccgaaggt caggccgggg tcgagaacat
     1321 gctgaaggtg ctgcgcgccg atgccgacgt ggcgcttgcg ctgaccggca gcaactcggt
     1381 caccgaactc ggcccggatt cgctggcctg agccggctgg ctcggtcggc caggacgaaa
     1441 cgggcggcag gcataaccac cggcagatag accctgcgtc cgcgtttgcg aatctcgaat
     1501 atctgtgttg ccgggccgaa gccgagccgc accgtgaaga tcccgcggcg gttgtgcctg
     1561 accttgcggt ttgaagggct gccgccgcaa agaggtacgg acttgagggt gagggaccct
     1621 ttccggcgga ttccggagag tctggccctc acctgttcgg ctgcgggcag gggcttgcgg
     1681 aagagcactc ttatcgcgct gcttgcccgc atccgcttcg acgaagaact cccgaccttc
     1741 aggctgtaac gggcggtctt gcgtgccttc tttgaccgtg gcgcggctac ggagacctcg
     1801 atgatcccgt ccgtcgccac cttcttcgac cccacctttc ggccattacg gagaaccttg
     1861 gccgt
//
LOCUS       DAOTIP010000452         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74067_length_1860_cov_3.326316, whole genome shotgun sequence.
ACCESSION   DAOTIP010000452 DAOTIP010000000
VERSION     DAOTIP010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74067_length_1860_cov_3.326316"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="ccsA"
                     /locus_tag="P5138_08560"
     CDS             complement(<1..539)
                     /gene="ccsA"
                     /locus_tag="P5138_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRV60669.1"
                     /translation="MDSSRYAVYAFCALMVICVVVIEHAFLTNDFSFNVVATHSSIET
                     PTFYKFTAMWSSQEGSLLLWAFILSIVSTISLAATRRKLRDLVPWATAVMMGIAVFFT
                     GLMLLGGDVNPFTTISPVPADGTGLNPLLQHPSMIIHPPMLYTGYVSLTVPFAFAIGA
                     LISRRVDAAWIKSTRGFAL"
     gene            complement(631..1032)
                     /locus_tag="P5138_08565"
     CDS             complement(631..1032)
                     /locus_tag="P5138_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRV60670.1"
                     /translation="MDVQRKRQVRLFVALGAALLLATGLIYTSFNASSEARQPSELTD
                     AVPGKSYEMTGKVVKGSVVEGNDRLKFSVKDRDGTGQALPVTYSGVVPDPFREGREIV
                     LTGTVQNGVFVGEKDTLVTKCPSKFSEKKNQ"
     gene            1093..1797
                     /locus_tag="P5138_08570"
     CDS             1093..1797
                     /locus_tag="P5138_08570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV60671.1"
                     /translation="MSEEKVLLITGASSGIGRATARLLASERRLVLAGRRVEPLEELA
                     AELGGPERALVVGCDVGEWDQVETMTAATLEAYGRIDEVFANAGFGATRGFLEESPEH
                     WKSMVLTNVLGTALTIRSTLPHLLERGSGHFLVTSSIAGRRVLPGSLYSATKFATTAI
                     GEALRQELRGRDDGRKIKVTLIEPGMVDTDFFDNRPGDSALADEDIARAIQYALSQPD
                     GVDVNEILIRPITQAL"
BASE COUNT          354 a          593 c          626 g          287 t
ORIGIN      
        1 atcagggcga agccgcgggt cgacttgatc caggccgcgt ccacccggcg gctgatcagg
       61 gcaccgatcg caaaggcgaa gggcacggtg agcgacacat accccgtgta gagcatcggc
      121 ggatggatga tcatggacgg gtgctggagc agcgggttga gtcccgtgcc gtcggccggg
      181 accggcgaga tcgtggtgaa tgggttgacg tccccgccga gcagcatcag cccggtgaag
      241 aagacggcga tccccatcat caccgcggtc gcccagggca cgaggtcgcg cagcttgcgc
      301 cgggtcgcgg ccagcgaaat cgtcgacaca atcgagagga tgaaggccca gagcagcagc
      361 gagccttcct ggctcgacca catcgccgtg aacttgtaga aggtcggcgt ctcgatcgac
      421 gaatgggtgg cgaccacgtt gaagctgaag tcgttggtca ggaaggcgtg ttcgatcacg
      481 accacgcaga tcaccatcag ggcgcagaat gcatagaccg cgtagcggga ggagtccacc
      541 cagcggcggt cgccccgggt tccgacgagc gcagccgccg ccgcaaagat tgcggtcagg
      601 aaggcaagcc agagcgcggc attgccgaaa ttactgattc ttcttctccg agaacttcga
      661 tgggcacttg gtcacgagtg tgtccttttc ccccacgaag accccgtttt gaacagtgcc
      721 cgtgagcacg atctcgcgcc cttcgcggaa ggggtcgggc acgaccccgc tgtaggtgac
      781 gggcaaggcc tggccggtgc cgtcacgatc cttcaccgaa aacttcagtc ggtcgttccc
      841 ctccacgacg gaacccttga ccaccttgcc ggtcatctcg tagctcttgc ccggcacggc
      901 atcagtcaac tcgctgggct gacgggcctc ggaagaagcg ttgaaggagg tgtagatcag
      961 tccggtggcc agaaggaggg cggcgcccaa ggccacgaac aggcgcacct gccgcttgcg
     1021 ctgtacgtcc ataacggtcg gaattatcgc aagccgcggc ggccggcgac ttccccctgc
     1081 cagaatcgca gcatgagcga agaaaaggta cttctcatca ccggagcgag cagcggcatc
     1141 ggacgggcca cagcccgcct cctggccagc gaaagacgcc tggtgctggc tggccgcagg
     1201 gtcgaaccgc tggaggaact tgcggccgaa ctgggcggcc cggagcgggc actggtggtc
     1261 ggctgcgacg tcggggaatg ggatcaggtc gagacgatga cggcggccac gctggaggca
     1321 tacggccgga tcgacgaggt cttcgccaac gccggcttcg gtgccacccg cggcttcctc
     1381 gaggagtcac cggaacactg gaagtcgatg gtcctgacca atgtgctggg taccgccctg
     1441 accatccgtt cgaccttgcc ccacctgctc gaacgcggca gcggccactt cctggtgacg
     1501 agttcgatcg ccggccgtcg cgttcttccg gggtcgctct attcggcgac caagttcgcc
     1561 accaccgcaa tcggcgaggc gctgcgccag gaactccggg gccgcgacga cgggaggaag
     1621 atcaaggtga ccctgatcga gcccggcatg gtcgataccg acttcttcga caaccgtccg
     1681 ggcgactcgg ccctggccga cgaggacatc gcccgggcca tccagtacgc gctcagtcag
     1741 ccggatggcg tggacgtgaa cgagatcctg atccgcccga ttacacaggc gctgtaagcc
     1801 cggcgcgggg ccgcggtgcc gggcaggcat ccccgggacg ggacgcgcac ggcattcagc
//
LOCUS       DAOTIP010000453         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74118_length_1859_cov_3.809867, whole genome shotgun sequence.
ACCESSION   DAOTIP010000453 DAOTIP010000000
VERSION     DAOTIP010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74118_length_1859_cov_3.809867"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="P5138_08575"
     CDS             complement(<1..1092)
                     /locus_tag="P5138_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRV60672.1"
                     /translation="MPSGPEDITLARAAAKGDGPAFASLYESYERPIFNYLLRLLGDR
                     HEAEDATQEAFLRVMAKLPELDLENLQFGPYLYTAARNAGYDVIAKRKRMEPSGAIPE
                     EAEDPFRDGNSDLETDPERSALAESQEAAVQAANGRLPERQREVLALRELDRMSYDEI
                     GSVMEMKPNAVAQLISRARIALRKEMRIGAAASVAFATADCERAHGELACRQDDQTTG
                     FDPAWLDAHLADCGNCRVAGEEMAEAGVSYRAWAPVLPAAWLFREVMAKASGINGHDW
                     SAVERPGSSGSEGGAGTSGGAGGAAAVGSWSRGRRAILAGAGSVLVAAVLATVLMAEA
                     DAPERVAAVRDSVVRPQSVLRKAEARAAGR"
     gene            complement(1156..>1859)
                     /locus_tag="P5138_08580"
     CDS             complement(1156..>1859)
                     /locus_tag="P5138_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60673.1"
                     /translation="YFRAGTDALGNELWRTDGNTVEMVGDIAPGEEDSGPAGLIAFGG
                     RVYFSADDDEHGVELWATDGGKPYMVRDILPGDGSGSPWEFTPYAGSLYFSADDGTHG
                     SEMWRVTADNSVKVKVPASTIKVDLSGRARVAVRCAQSEISGPCRGTVIVKTKVKVKV
                     GGKRRQLLIGKGKFSAAAGKAGQATLKLTRQTRKLLRKYPAARKVTVRVKAQDSVGNK
                     TALKKAAGLTGPGAR"
BASE COUNT          256 a          662 c          604 g          337 t
ORIGIN      
        1 cctgccggcc gctctggctt cggcctttct caggacgctt tggggacgga ccacggaatc
       61 cctgaccgca gcaacccgct cgggtgcgtc cgcttccgcc atcagcaccg tggcaaggac
      121 cgccgccacc agaaccgaac cggctccggc caggatggcc cggcggccgc ggctccagct
      181 accaacggcg gccgcaccgc ccgctccgcc cgatgtcccg gcgccccctt cggaaccgga
      241 ggatccgggc ctctcgacgg ctgaccagtc gtgtccgttg atcccgctgg cctttgccat
      301 gacttcccgg aacagccagg cggccggaag gactggtgcc caggcgcggt aggaaacgcc
      361 ggcctcggcc atctcctcac cggcgacacg gcagtttccg cagtcggcca ggtgggcgtc
      421 cagccaggcc gggtcgaagc cggtcgtctg gtcgtcctgc cggcaggcaa gttcaccatg
      481 cgcgcgttcg cagtcggccg tggcgaaggc gaccgaggcg gccgctccga tcctcatttc
      541 cttgcgcagg gcgatcctcg cccgtgagat cagctgcgcc acggcgttcg gcttcatctc
      601 catcaccgag ccgatctcgt cgtagctcat gcggtcgagt tcccgcaggg ccaggacttc
      661 ccgctgacgt tcggggagcc tcccgttggc cgcctgtacg gctgcctcct gggactccgc
      721 cagggccgac cgttccgggt cggtctcgag atcggagttc ccgtcgcgga agggatcctc
      781 cgcctcctcc ggaatcgcgc ccgacggttc cattctcttg cgcttggcga tcacgtcgta
      841 cccggcgttc ctggccgcgg tgtagagata cgggccgaac tggaggttct cgaggtccag
      901 ttccggcagt ttcgccatga cccggaggaa agcttcctgg gtcgcgtcct cggcttcgtg
      961 tcggtcgccg aggaggcgga ggaggtaatt gaagatcggg cgctcgtacg actcgtaaag
     1021 actcgcgaat gccgggccat cccccttggc ggcagcccgg gcgagagtga tgtcctcggg
     1081 cccggagggc atgagattaa tctaacaccg ctccgggccc gagtcatcgt cctggctacc
     1141 ttccctgcct cgcactcacc gggctcccgg cccggtgaga cctgcggcct tcttgagggc
     1201 cgtcttgttt ccgacgctgt cttgcgcctt gacccggacc gtcaccttgc gggctgccgg
     1261 gtacttgcgc agcagcttcc gggtctggcg ggtcagcttc agggtggcct gtccggcctt
     1321 cccggccgcg gccgagaact tgcccttgcc gatcaggagc tgccggcgct tgccgccaac
     1381 cttgaccttc accttcgtct tcacgatcac cgtgccgcgg cagggaccgg agatctcgct
     1441 ctgggcgcag cgcacggcga ccctggcccg acccgagagg tcgaccttga tcgtgctggc
     1501 cggcaccttg accttgaccg agttgtcggc agtgaccctc cacatctcgg agccatgggt
     1561 cccgtcatcg gccgagaagt agagcgaacc ggcgtagggg gtgaattccc atgggctgcc
     1621 cgaaccgtcg cccggcagga tgtcccggac catgtacggc ttgccgccgt cggtcgccca
     1681 gagctcgact ccgtgttcgt cgtcgtcggc gctgaagtag accctgcctc cgaatgcgat
     1741 gagcccggcc gggccggaat cctcctcgcc gggggcgatg tctccgacca tttcgacggt
     1801 gttgccatcg gtccgccaga gctcgttgcc cagggcatcg gtgccggccc ggaagtaga
//
LOCUS       DAOTIP010000454         1858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74193_length_1858_cov_2.977815, whole genome shotgun sequence.
ACCESSION   DAOTIP010000454 DAOTIP010000000
VERSION     DAOTIP010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74193_length_1858_cov_2.977815"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="P5138_08585"
     CDS             complement(<1..591)
                     /locus_tag="P5138_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HRV60674.1"
                     /translation="MSESTPGYVLLEDGSRFEGALCGNVGGGVGEVVFNTAMTGYQEA
                     VTDPSYAGQIITFTYTMIGNYGVSAEAMESDRPHARGVIMREGRNGADAATAEGGWLD
                     WLEANGVWAITGVNTRALVRHIRDKGAMRGGIFPASVTSDEAMGRIEAEPSMAGSDFA
                     SKVSPEEPIEHPGQGPHVVALDTGIKASIVNQFLERD"
     gene            complement(588..>1858)
                     /locus_tag="P5138_08590"
     CDS             complement(588..>1858)
                     /locus_tag="P5138_08590"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRV60675.1"
                     /translation="AAGISGARDILIEGGSISRLAEAGTLEAADGVEVIEAEGLHVFP
                     AFFDPHVHFRTPGQEYKEDLETGTRSAAAGGYAGVIAMANTSPRVSSPEQIEALRDRA
                     SHVASVPVGFTATVTTDMKGTELTEMAELREAGAVGFTDDGLPIASARILRRALQYQA
                     MTGGRIALHEEDPELSGKGVMNEGVVSSELGLRGVPSISESTMIGRDAAIAGYEGGAI
                     QIQHLSAIESIEQIRRAKEDGVDITCEVTPHHLCLTDEAVRDLDASKHKMNPPLRTES
                     DRQALIEALIDGTIDCIATDHAPHAIHEKEVPYEAANMGVTGLETAFSSIYTELVLPG
                     RITLDLLVEKLGSGGVPFGIEPFTLIEGSRANLCLVDLEAEWVVGEDGYESRSVNSWC
                     AGETLRSRVLVTLADGQVAFRLRTFSLGVA"
BASE COUNT          287 a          632 c          600 g          339 t
ORIGIN      
        1 gtccctttcc aggaactggt tgacgatcga agccttgatc ccggtgtcga gagcgaccac
       61 atggggcccc tggccgggat gctcgatcgg ctcctccggg ctgaccttgg aagcgaaatc
      121 cgaaccggcc atagagggct cggcctcgat ccgtcccatc gcctcatcgc ttgtgaccga
      181 ggccgggaag atgccgccgc gcatcgcacc cttgtcccgg atgtggcgga ccagggcgcg
      241 ggtgttcacc ccggtgatcg cccagacccc gtttgcctcg agccagtcga gccagccacc
      301 ctcggcggtg gccgcatccg cgccgtttct gccctcgcgc atgatcacgc cgcgggcgtg
      361 gggacggtcg gattccatcg cttccgccga caccccgtag ttgccgatca tcgtgtaggt
      421 gaaggtgatg atctgacccg cataggaggg gtcggtgacc gcctcctggt agccggtcat
      481 ggccgtgttg aagacgactt cgccgacccc gcccccgacg ttgccgcaaa gcgctccctc
      541 aaaacggctg ccgtcttcga gcaggacgta tcccggtgtc gactcgctca tgccactccc
      601 agactgaaag ttctcagacg gaaagcaact tgcccgtcgg ccagggtcac cagcacccgg
      661 ctgcggagcg tctcgccggc acaccaggag ttgaccgagc ggctctcgta gccgtcttcc
      721 ccgaccaccc attcggcttc caggtcgacc aggcagaggt ttgcccggct gccttcgatc
      781 agggtgaacg gctcgatgcc gaagggcacc ccgccggatc cgagcttctc gaccagcagg
      841 tcgagcgtga tccggccggg cagcaccagt tcggtgtaga tcgaggagaa ggcggtttcc
      901 agtcccgtca cgcccatgtt ggcggcctcg tagggcactt ccttctcatg gatggcatgg
      961 ggtgcgtgat cggtcgcgat gcagtcgatc gtcccgtcga tcagcgcctc gatcagggcc
     1021 tgccggtcgg actcggtcct gagcggcggg ttcatcttgt gtttcgaggc gtcgagatcg
     1081 cggaccgcct cgtcggtcag gcacaggtgg tgcggggtca cctcgcaggt gatgtccaca
     1141 ccgtcttctt tcgcccgccg gatctgctcg atcgattcga tcgcggagag atgctggatc
     1201 tggattgccc cgccttcgta gccggctatc gccgcgtcgc ggccgatcat cgtcgattcg
     1261 gagattgagg gaacgccccg gaggccgagt tccgacgaga cgacgccttc gttcatcacg
     1321 cccttcccgg agagttcagg atcctcctcg tggagggcaa tgcgcccgcc ggtcatggcc
     1381 tggtactgga gcgcccggcg caggatccgg gcgctggcga tcggcagtcc gtcgtcggtg
     1441 aaaccgaccg ccccggcctc acgcagttcc gccatctcgg tcagctctgt gcccttcatg
     1501 tccgtggtca cggtggcggt gaacccgacc gggaccgagg ccacatgaga ggcccggtcg
     1561 cgcagcgcct cgatctgctc ggggctcgaa acacgcggcg aggtgttcgc catcgcaatc
     1621 acgcccgcgt aaccgcccgc ggcagccgac cgggtgcccg tttcgaggtc ctccttgtac
     1681 tcctgtccgg gtgtgcggaa atggacatgg ggatcgaaga aagccgggaa tacatggagc
     1741 ccttccgcct cgatcacttc caccccgtcc gcggcttcga gcgtgccggc ctcggcgagg
     1801 cgggaaatgg acccgccctc gatcaggatg tccctggcac cggagatgcc cgccgcgg
//
LOCUS       DAOTIP010000455         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74227_length_1857_cov_3.648169, whole genome shotgun sequence.
ACCESSION   DAOTIP010000455 DAOTIP010000000
VERSION     DAOTIP010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74227_length_1857_cov_3.648169"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /gene="dnaA"
                     /locus_tag="P5138_08595"
     CDS             <1..1594
                     /gene="dnaA"
                     /locus_tag="P5138_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRV60676.1"
                     /translation="GLVTNGEVWTLVHAAPNQTPAFVTWYAELWGEEPLTLRSFWELL
                     SVGRTLGAEDERLEALIAESARDQQEVTDQLGRQVRQAVELLVSALEREDRARNRQLL
                     AGVPEARLFDAAWKMWIEPIRPAAVRGTTLYLSAADPIRTWVERRYIEVLQEVLVRSD
                     SHLTSISFEAPPETERVQAPTEPVGPNPTHDFDRFVIGPGNHLAHAAALAVSEAPSEA
                     YNPLFLHGPPGLGKTHLLGAIANYLALHVPELNVLYTNAESFTAEFVTALREQGTDEF
                     KRRYRNLDVLLIDDIQFIAGKHHTEEEFFHTFNALYDAGSQIVISADRSPSEISTLAD
                     RLGDRFEWGLTVPLEAPNLATRLTVLRHLVTEAGLEVTDQDALTLLARRVQTNLRQLR
                     GALTRTIAESSLASAPITYELISQVFPATEAAPAVPVTPESIRSTVASHFRTDVENLQ
                     SRRRDRNTATARHVAIFLTREMTSLSLPQIGGLYGDRDHTTVLNSIERIDAALGQDAE
                     ITTSIEMLRAEIHSPGENFPNE"
BASE COUNT          353 a          681 c          514 g          309 t
ORIGIN      
        1 gggcttggtc acgaacggcg aggtctggac gctggtgcat gccgccccga accagactcc
       61 ggccttcgtc acctggtacg ccgagctgtg gggcgaggag ccgctgacgc tgcggtcgtt
      121 ctgggagctt ctgtcggtag ggcggacgct gggggccgag gacgagcgcc tggaggccct
      181 gatcgccgag agcgccaggg accagcagga ggtcaccgac cagctcgggc gccaggtccg
      241 ccaggccgtg gagctcctgg tctccgcgct ggagcgggag gaccgcgccc gcaaccgcca
      301 gctcctggcg ggagtccccg aggcccggct cttcgacgcc gcctggaaga tgtggatcga
      361 accgatccgc ccggccgcgg ttcgcggaac gaccctttac ctcagtgccg ccgacccgat
      421 ccggacctgg gtggagcgac gctacatcga ggtcctccag gaggtcctcg ttcgcagtga
      481 ctcccacctg acctcgatca gcttcgaggc tccgcccgaa accgagcggg tccaggctcc
      541 taccgagccg gtcggtccca accccaccca cgacttcgac cgctttgtaa tcggccccgg
      601 caaccatctc gcccacgcgg ccgccctggc cgtctccgaa gctccgtccg aggcctacaa
      661 ccccctcttc ctccatggtc ctcccggcct cggaaagaca cacctactgg gtgcgatcgc
      721 caactacctt gcgctccacg ttcccgagct caacgtcctc tatacgaacg ccgagtcgtt
      781 caccgccgag ttcgtgacag cactcaggga gcagggaaca gacgaattca agcgccgata
      841 ccggaacctg gacgttctcc tgatcgacga catccagttc atcgccggca agcaccacac
      901 cgaggaagag ttcttccaca cttttaacgc cctctacgac gccggaagcc agatagtcat
      961 ttccgccgat cgttctccct cggagatctc gactctcgcc gaccggctag gagatcgctt
     1021 cgaatgggga ctgaccgttc ccctcgaggc tccgaacctc gctacccgcc tgacggttct
     1081 ccgccacctg gtcaccgaag ccggactcga agtcaccgac caggacgccc tcactctcct
     1141 ggcccgacgg gtccagacca accttcggca actgcgcggt gccctgacca gaaccatcgc
     1201 cgaatcctcg ctggcttctg ccccgatcac atatgagctg atcagccagg tctttccggc
     1261 cacggaagcg gccccggccg tccccgtcac gcccgaatcg atccgaagca ccgtcgcatc
     1321 gcacttccgt accgacgtcg agaatctcca gtcccgccgg cgcgaccgca acacggcgac
     1381 cgccaggcat gtcgccatct tcctgacccg ggaaatgacc tccctctccc tgccccagat
     1441 cggcggactc tacggcgacc gggatcacac cacggttctc aactcgatcg agcgaatcga
     1501 cgcggcactc ggccaggacg cggaaatcac cacctcgatc gagatgctcc gggccgaaat
     1561 ccacagcccc ggggagaact tcccgaatga atgagtcagc cgccgagccc actgaaagaa
     1621 tcccggtttc cccacagttt ccccacagca gattcggctt tacagagcct tttccaacgg
     1681 ttcttcgctt tactccacag aaccagaaca cttaatcgag gtattcggat ttgaagttct
     1741 cagtcaagaa caacgaactg gccggcaaac tcgccctggt cgcccgcaca cttgccagcg
     1801 gtggtgtaaa ccccgcgctc ggcggaatcc tgatcgaggc gaccgaagga cgactgt
//
LOCUS       DAOTIP010000456         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74537_length_1852_cov_2.544240, whole genome shotgun sequence.
ACCESSION   DAOTIP010000456 DAOTIP010000000
VERSION     DAOTIP010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74537_length_1852_cov_2.544240"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="P5138_08600"
     CDS             <1..697
                     /locus_tag="P5138_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60677.1"
                     /translation="FLARPLNPLTFVASMSLTGGPGKSDSDVGRLRTKLDRYPDLKFK
                     LDPSLDWDQGLIDELVGTGAVNTLDLKGQYKGTVVDIETDPQLYRRLLASFPEAWIED
                     PDLTPETREVLKDDWHRVTWDAPIHSVGEIAALEHKPTVINIKPSRMGGLQSTFDAYD
                     YCYANGIGAYGGGQWELGVGRDQIQYLAALFHPDNPNDTSPVGYNAAEPEPGLPTSPM
                     KVPGNGFGKPDQS"
     gene            789..1322
                     /locus_tag="P5138_08605"
     CDS             789..1322
                     /locus_tag="P5138_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRV60678.1"
                     /translation="MAKKKSFAKALNAQISNEFAASQQYVAMAVYYDSETLPRLAAFF
                     YRQAIEEREHAMMMIQYLLDVDEEVEVPDIKSQPTKYEDGITPVKEALAQEQRVSDEI
                     FGLFELARELKDYRAEHFMQWFVKEQVEEVALMGDLLNVVERSSENLLMAEEYIARES
                     LGEEGEDPTAPPPAGGE"
     gene            1520..>1852
                     /locus_tag="P5138_08610"
     CDS             1520..>1852
                     /locus_tag="P5138_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60679.1"
                     /translation="METCNRQAFRLAAIAAAMAALAFVLTFGVNRAAAADPAVFDVGA
                     ASSSINPDSPQYVAGYGYKVGPMQTTHDDLEARAFVVGKGDKAVAFVSVDLVGWFAAY
                     DGVNAPYGI"
BASE COUNT          365 a          606 c          597 g          284 t
ORIGIN      
        1 attccttgcc cggccgctca atccgctcac cttcgtcgcc tcgatgagcc tgaccggcgg
       61 ccccggcaag tccgactccg atgtcggcag gctgcggacc aagctcgacc gttacccgga
      121 cctgaagttc aagctggacc cgtcgctgga ctgggatcaa ggcctgatcg acgagctggt
      181 cgggaccggc gcggtaaaca ccctcgacct gaagggccag tacaagggga cggtcgttga
      241 catcgagacc gaccctcagc tctatcggag gctgctcgcc tccttccccg aagcctggat
      301 cgaggacccc gacctgaccc ctgaaacccg cgaggtactc aaggacgact ggcatcgcgt
      361 gacctgggac gcgccgattc attcggtcgg ggagatcgcc gcgctcgagc acaagccgac
      421 cgtgatcaac atcaagccct cccggatggg cggcctgcaa tcgaccttcg atgcctacga
      481 ctattgctac gccaacggca tcggcgctta cggcggcggc cagtgggaac tgggggtcgg
      541 acgggaccag atccagtacc tggccgccct gtttcacccc gacaacccga atgacacctc
      601 gccggtcggc tacaacgccg ccgagccgga gccgggactg ccgacgagtc cgatgaaggt
      661 ccccggaaac ggctttggca agccggatca gagttagagc catctaactc ctcctgcggc
      721 agaccgaagg ccgattctgc ggtacggttg gccaggcaac ccgcagaacc ccaggacaag
      781 gagacggcat ggccaagaag aagagtttcg ccaaggcgct caacgcccag atctcgaacg
      841 agttcgcagc ctcccagcag tacgtggcga tggccgtcta ctacgacagc gagaccctgc
      901 cccgactggc cgcgttcttc taccgccagg cgatcgagga gcgggagcac gcgatgatga
      961 tgatccagta cctgctcgac gtcgacgagg aggtggaggt tcccgacatc aagtcccagc
     1021 cgaccaagta cgaagacggg atcacgccgg tcaaggaggc cctcgcccag gaacagcggg
     1081 tctccgacga gatattcggg ctcttcgaac tcgcccgcga actcaaggac taccgggccg
     1141 aacacttcat gcagtggttc gtgaaggagc aggtcgagga agtcgcgctg atgggcgatc
     1201 tgctgaacgt ggtcgaacgg tcgagcgaaa acctgctgat ggccgaggag tacatcgccc
     1261 gcgagagcct gggcgaggag ggcgaggacc cgaccgcccc gccgccagcc ggcggtgagt
     1321 gagctccggc ccggccgctc tgagctccgg cccgaccggt gaatcaaggg gtccgggccg
     1381 gcaaatgaat taacccggtc ggtactagaa agggtctcct gccctgcggt aggctcctgc
     1441 caaggaggag ttggccgagg gtcggctcaa aaccgggagg ccggcgcgga gcgctggccg
     1501 agaggagatt tgcatcaaca tggagacctg taacaggcag gcgtttcggc ttgccgcgat
     1561 agccgccgcg atggcagcgc tcgcctttgt cctgactttc ggagtcaacc gcgcggcagc
     1621 ggccgacccg gcggtattcg acgtcggcgc cgcatcgtcg agcatcaacc ccgactcgcc
     1681 gcagtacgtg gccggctacg gctacaaggt gggcccgatg cagaccaccc atgatgatct
     1741 cgaggcccgt gcattcgtgg tcggcaaggg cgacaaggcc gtcgccttcg tctcggtcga
     1801 cctggtcggc tggttcgccg cctacgacgg cgtgaacgcc ccctacggaa tc
//
LOCUS       DAOTIP010000457         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74584_length_1851_cov_3.080735, whole genome shotgun sequence.
ACCESSION   DAOTIP010000457 DAOTIP010000000
VERSION     DAOTIP010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74584_length_1851_cov_3.080735"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1053)
                     /locus_tag="P5138_08615"
     CDS             complement(112..1053)
                     /locus_tag="P5138_08615"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRV60680.1"
                     /translation="MGKLFFFTGEAIRAIKRNVAPTTAAVVTTVLTALLLGVLIPVFQ
                     TAQSKGEDVRGQLELRTFLYDDATKAETQALQQKLKAIPHVESVTYLNKAAAKKEFMN
                     SDFGKANPDALKQLGGRNPLPANFIIKPDDASNLDSIRAAIQTSGGGTQSNGSGSASQ
                     AKYISPIIASVDDRQEDARDIEQVTGALKLILTVITVLLIGASLLLIGNTIRLSIYTR
                     RREIEVMRLVGATRWFIRWPFMIEGVVVGILGGAIAILVLWIGKVTVVDPLSDKFALM
                     NAQDSMSFVALVGCLFIAAILVSAIGSGLTMRRFLKV"
     gene            complement(1053..1814)
                     /gene="ftsE"
                     /locus_tag="P5138_08620"
     CDS             complement(1053..1814)
                     /gene="ftsE"
                     /locus_tag="P5138_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRV60681.1"
                     /translation="MRDVSKVYPGGHLAVDRVSLAINRGEFVFLVGPTGCGKSTLIKM
                     LIRELEPTDGTISIAGRDINQLPEKKVPQLRRNIGTVFQDFKLLPSRTVYDNVAYALQ
                     VIGASRAEIRQKVPETLRLVGLSTKLHNYPDELSGGEQQRVSVARAFVNHPPLLLADE
                     PSGNLDPVTSVGIMQLLYRINKTGTTVVVVTHDREMVDRMRRRVIELYEGRVIRDEVA
                     GGYTEESTTEFGVRMRAEMGVGVEGHTNGNSKGAH"
BASE COUNT          330 a          552 c          614 g          355 t
ORIGIN      
        1 acgatggtgc cgagcagcat gccgccgatt accgcccaga cggcaacgat tccggtcttc
       61 aaatcgccat agggtcgatg ttgcccgtgc ccggaagcgg gcccgaaccg ctcagacctt
      121 gaggaagcgg cgcatggtca gtccggagcc gatcgccgaa accaggatcg cggcgatgaa
      181 cagacagccg acgagggcca cgaaggacat tgagtcctgc gcattcatca gggcgaattt
      241 gtccgaaagg gggtcgacga cggtgacctt gccaatccac agcaccagta tcgcaatcgc
      301 gccaccgagg atgcctacga ccaccccttc gatcatgaag ggccagcgaa tgaaccagcg
      361 ggtggcaccg accagacgca tgacctcgat ttcgcgacgg cgggtgtaga tcgagagacg
      421 gatcgtgttg ccgatcagga gcagggaggc accgatcagc aggaccgtga tcacggtgag
      481 aatcagcttc agggccccgg tcacctgctc gatgtcccgg gcatcttcct gacggtcatc
      541 gaccgaggcg atgatcgggg agatgtactt cgcctggctg gccgatccgg agccgttgga
      601 ctgggtccct ccgccagaag tctggatcgc cgcacggatg ctgtccaggt tggaggcatc
      661 atccggcttg atgatgaagt tggccgggag cggattcctg ccgcccagct gcttgagagc
      721 atccgggttc gccttgccga agtcactgtt catgaactcc ttcttggcgg cggccttgtt
      781 cagataggtg accgactcga catgcggtat ggccttcagc ttctgttgca gggcctgggt
      841 ttcggccttg gtcgcgtcgt catagaggaa ggtgcgcagc tcgagctggc cgcggacgtc
      901 ttcgcccttg ctctgggcgg tctggaagac cgggatgagc acgccgagca ggagcgcggt
      961 gagcacggtg gtgaccacgg ctgccgtggt cggggcgacg ttccgcttga tcgcgcggat
     1021 cgcttcgccg gtgaagaaga aaagcttgcc catcagtgtg ctcccttgga atttccgttg
     1081 gtgtggcctt ctactccgac gcccatctcg gccctcatgc ggacgccgaa ctcggtcgtg
     1141 gactcttcgg tgtatccgcc ggccacttca tcgcgaatga cccggccctc gtagagctcg
     1201 atcacgcgac ggcgcatgcg gtcgaccatt tcgcggtcat gggtgacgac gacgaccgtg
     1261 gttccggtct tgttgatccg gtagagaagc tgcatgatcc cgaccgaggt caccgggtcg
     1321 aggtttccgc tgggctcatc ggccagcagc aggggcggat ggttgacgaa ggctctcgcc
     1381 accgaaacgc gctgctgctc gccgccggac agctcgtccg ggtagttgtg aagcttggtc
     1441 gagagaccga ccagtcgcag ggtctctggc accttctggc ggatttccgc cctggaggca
     1501 ccgatcacct ggagggcgta ggcgacgttg tcgtaaacgg tgcggctggg aagcagcttg
     1561 aagtcctgaa agacggtgcc gatattgcgg cgaagctgcg gaaccttctt ctccgggagc
     1621 tggttgatgt cgcgtccggc gatcgaaatc gtcccgtcgg tcggctcgag ttcacggatc
     1681 agcatcttga tcagtgtcga cttgccgcag ccggtcggtc cgaccaggaa gacaaactcg
     1741 ccccggttga tggcgagcga gacccggtcg acggcgagat ggccaccggg gtagaccttg
     1801 ctgacgtcgc gcatctcgat gatcggcgct ccaccctttc ggggccgctc g
//
LOCUS       DAOTIP010000458         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74631_length_1850_cov_4.193315, whole genome shotgun sequence.
ACCESSION   DAOTIP010000458 DAOTIP010000000
VERSION     DAOTIP010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74631_length_1850_cov_4.193315"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="P5138_08625"
     CDS             <1..1322
                     /locus_tag="P5138_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60682.1"
                     /translation="DADRQWIITAYALAFGSLLLLGGRLADYFGRKRTLIAGLLGFAG
                     ASAVGGLAPGFEVLVGARALQGMFGALLAPSALSTLTNTFTIPSERAKAFGVYGALAG
                     GGGAIGLLLGGVLTEYLDWRWTMFVNVAFAIPAAIGLITLLVNDSRQGEMRVDIPGAV
                     AASSGLFAIVFGFSRAESDGWTAAVTLSSLAIGVGLLALFVLIERRSAMPLLPARVVA
                     DRNRAGSYLAVGVAGASMFGVFLFLTYYLQQTLGMSPVETGLAFLPMIAFLMVGSISS
                     STRLMPRFGPRPLVPTGMVLAAAGMVWLTGVEVDSSYAGAVLPGLALVGAGFGLVIAP
                     SMASATLGVAASDSGVASAMVNTSQQVGGSVGTALLSTISASAVTAFASGKVPSPELA
                     AQAAVHGYTTAFWVSAAMLMAGAVVTAVTLRSGAPQTDDGHGLAMAH"
     gene            complement(1375..>1850)
                     /locus_tag="P5138_08630"
     CDS             complement(1375..>1850)
                     /locus_tag="P5138_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRV60683.1"
                     /translation="TRKQDKGCGGVPAGFLNAQYCSINQTISWDGNWLYWVLYRHLGE
                     AAVSFLLAHEYGHLVQDQLGIDNRFHQTIEAELNADCLAGAWLGAVNARITRLDQVDY
                     QSLYDGMLEVADPQGIPWNDPTAHGTAAERGRALDFGATHGPNACLKQLGPGFTA"
BASE COUNT          247 a          618 c          628 g          357 t
ORIGIN      
        1 gtgatgccga ccggcaatgg atcatcaccg cctacgcact cgcgttcggc agcctgctgc
       61 tgctcggcgg ccggctggcc gattacttcg gccgcaaacg gaccctgatc gccggtttgc
      121 tcggatttgc cggagcctcc gccgtcggcg gactcgctcc cggcttcgag gtactggtcg
      181 gggcccgggc cctgcaggga atgttcgggg cattgctggc gccttcggcc ctttccaccc
      241 tgaccaacac cttcaccatt ccctccgagc gggcgaaggc tttcggcgtc tacggcgccc
      301 tggctggagg gggcggcgcg atcggcctgc tgctgggggg tgtcctcacc gaatatctcg
      361 actggcgctg gaccatgttc gtcaatgtcg cgttcgccat tcctgcggcg atcggtctga
      421 tcaccctgct ggtcaacgac tcacggcaag gcgaaatgcg ggtggacatc cccggtgccg
      481 tggctgcttc gtccggtctc ttcgcgatcg tcttcggatt ctcgagggcc gagtccgatg
      541 gctggaccgc agccgtcacg ctgtccagtc ttgcgatcgg cgtcggcctg ctggccctgt
      601 tcgtgctgat cgagcgccgc agcgcgatgc cgctgctccc ggctcgcgtg gtagccgatc
      661 gcaaccgggc cggctcgtac ctcgcagtcg gagtggcggg agccagcatg ttcggggtct
      721 ttctgttcct gacctactac ctgcagcaga ccctcgggat gagcccggtg gagaccggac
      781 tcgccttcct tccgatgatc gccttcctga tggtcggttc gatcagttcg agcaccaggt
      841 tgatgccccg gttcgggccg aggccgctgg tcccgaccgg aatggttctg gccgcggccg
      901 gcatggtctg gctgaccggg gttgaagtcg attcgagcta tgccggggcg gtcctgccgg
      961 gactcgctct ggtcggcgcc gggttcgggc tggtcatcgc cccttcgatg gccagcgcaa
     1021 ccctgggtgt cgcggccagc gattcgggag tggcctcggc catggtcaat acctcccagc
     1081 aggtcggcgg atcggttggc accgccctgc tctcgacgat ctccgcttcc gcggtcaccg
     1141 ccttcgcctc ggggaaggtc ccttcacccg agctggctgc acaggccgcg gtccacgggt
     1201 acacgaccgc cttctgggtg tcggcggcaa tgctcatggc cggcgcggtg gtcaccgcgg
     1261 tcacccttcg cagcggcgcc ccgcagaccg acgacggcca cggcctggcg atggcccact
     1321 gaaccggacg ggacaggcag agcggcgggc gagcgccgct ctgtttgtcc gggctcaggc
     1381 cgtgaagccg gggccgagct gcttgaggca ggcgttgggt ccgtgcgtgg ccccgaaatc
     1441 gagtgcccgg ccgcgctcgg ccgcggtgcc gtgagcggtc ggatcattcc aggggattcc
     1501 ctgcgggtcg gcgacttcga gcattccgtc gtagagcgac tggtagtcca cctgatcgag
     1561 ccgggtgatc cgagcgttga ctgcccccaa ccatgccccg gcgaggcaat cggcgttgag
     1621 ttccgcctcg atcgtctggt gaaagcggtt gtcgataccc aactggtcct ggaccaggtg
     1681 gccgtattcg tgcgccagca ggaaggagac cgctgcttca ccgaggtggc ggtagaggac
     1741 ccagtacagc cagttgccat cccaggagat cgtctggtta atggagcagt actgcgcgtt
     1801 gaggaaaccg gcaggcaccc cgccgcaacc cttgtcctgc ttcctcgtcc
//
LOCUS       DAOTIP010000459         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74746_length_1848_cov_3.648076, whole genome shotgun sequence.
ACCESSION   DAOTIP010000459 DAOTIP010000000
VERSION     DAOTIP010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74746_length_1848_cov_3.648076"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="P5138_08635"
     CDS             complement(<1..1379)
                     /locus_tag="P5138_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60684.1"
                     /translation="MVSFALALLAGSTLLTAGSNAAGKGSGPVRLAGSGSSWKVLFPV
                     RLPLNVSGQKVNGRITINLPRGSWTSRWGFTAHAGRLRLGDQRRKFTFIKGVRVPKAV
                     AGVIARRHQAIRTTLKVGYRIPSAELSEVSTGGFASTQTGMPRAGAGFCETLPRLLVR
                     PSSNGAQRIALPACGRKLNWRIGESPRKGKVIRKGRAMLYRPSGGAGVDEFVIEGFRR
                     GRLTAKQRVQVRLLDGDASNISVVAFGDSVTAGFGYFGATGKQMGVTQLDDCRPGATT
                     FNDACSSNSTNRDSSIGTKPNYLPDFGLSRNISWAAQWTNQYGITSYRNYAVTGSAPS
                     DWLPGGQFESTLQSIEQQDPTYILMTMGANPLLSDVLFGVDNMGCALESDLFGDFRQC
                     VLDAFSAVDLNQKLNDLYTSLVSNSTSHIVVMQYHLAVPSTAIAYSATQLEMMTELLN
                     GVISDEAAQV"
     gene            1480..>1848
                     /locus_tag="P5138_08640"
     CDS             1480..>1848
                     /locus_tag="P5138_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60685.1"
                     /translation="MGARIIENGEIGISWVMDEPMARASHALTDGEKVWLIDPVDDEA
                     AMDRALSLGKPAVVFQLLDRHNRDCQEVADRLGVDLVALPLELRGTPFEAIEVVENRI
                     WKERALWWKKTKTLVVAEAIG"
BASE COUNT          345 a          585 c          582 g          336 t
ORIGIN      
        1 acctgtgccg cctcgtcgct gatcacgcca ttcaggagtt cggtcatcat ttccagctgg
       61 gtcgcgctgt aggcgatcgc ggtcgaaggt accgccaggt ggtactgcat gacgacgata
      121 tgcgaggtgg agttactgac cagactggtg taaaggtcat tcagtttctg gttcaggtcc
      181 accgccgaga aggcatccag cacgcattgg cggaaatcgc cgaaaaggtc cgattcgagg
      241 gcacagccca tgttgtccac gccgaacaga acatccgaga gcaggggatt ggcgcccatc
      301 gtcatcagga tgtaggtggg gtcctgctgc tcgatcgact ggagggtcga ctcgaactgg
      361 ccgcccggca gccagtcgct cggggccgaa ccggtcaccg cgtagttccg gtaactggta
      421 atcccgtact gattggtcca ctgcgcggcc caggaaatat tccttgagag accgaagtcc
      481 ggcaggtaat tcggtttggt cccgatcgaa ctgtcgcggt tcgtggaatt cgacgaacag
      541 gcgtcattga aggtggtcgc gccgggccgg cagtcgtcga gctgggtcac ccccatctgc
      601 ttgccggtgg caccgaaata gccgaagccc gcggtgaccg aatcaccgaa ggcgaccacg
      661 gaaatgttcg aggcatcgcc gtccagcagg cgcacctgaa ccctctgctt cgcggtcagg
      721 cgccccctcc ggaatccctc gatcacgaat tcgtcgaccc cggctccgcc cgagggccgg
      781 taaagcatcg cgcggccctt gcgaatcacc tttcccttgc gcggggattc gccgatcctc
      841 cagttcagct tgcgcccaca ggccggcagg gcgatccgtt gggccccgtt cgacgagggg
      901 cggaccagaa gcctcggcag ggtctcgcag aaaccggccc ccgccctggg cattccggtc
      961 tgggtcgagg cgaagccgcc ggtcgagacc tcggaaagtt cggcggacgg gatgcggtac
     1021 ccgaccttca gcgtcgttct gatcgcctga tgccttctcg cgatgacccc cgcgaccgcc
     1081 ttcgggaccc gcaccccctt gatgaaggtg aacttccggc gctggtctcc aagcctgagc
     1141 cttcccgcgt gggccgtgaa cccccatcgg gaagtccagc tgccccgagg gagattgatc
     1201 gtgatccgtc cgttgacctt ctgcccggac acgttcagcg gcagtcgcac cgggaagaga
     1261 accttccagc tggaaccgga accggcaagg cgaaccggac ccgaaccctt gccggctgcg
     1321 ttcgagccgg ctgtgaggag agtcgaccct gccagcagcg caagagcaaa cgagaccacc
     1381 cacacgagtc ccctgtgtct gaagtccgat ccccgcattt cgcaaacctt aaccctcgcg
     1441 gtagtcattc ggttcgcatg caggaataaa cttcccatcg tgggcgcgag gatcatcgag
     1501 aacggcgaga tcggaatcag ctgggtcatg gacgagccga tggcccgtgc ctcgcatgcc
     1561 ctcaccgatg gcgagaaggt ctggctgatc gatccggtcg atgacgaagc cgcgatggac
     1621 cgcgcacttt ccctcggcaa gcctgcggtt gtcttccagt tgctcgatcg ccataaccgg
     1681 gattgtcagg aggttgccga ccggctcggc gttgatctgg tcgcccttcc gctcgagttg
     1741 aggggcaccc ctttcgaggc gatcgaggtt gtcgagaacc ggatctggaa agagcgggca
     1801 ctctggtgga agaagaccaa gaccctggtc gtggccgagg cgatcggc
//
LOCUS       DAOTIP010000460         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74835_length_1846_cov_4.182021, whole genome shotgun sequence.
ACCESSION   DAOTIP010000460 DAOTIP010000000
VERSION     DAOTIP010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74835_length_1846_cov_4.182021"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..681
                     /locus_tag="P5138_08645"
     CDS             82..681
                     /locus_tag="P5138_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRV60686.1"
                     /translation="MSTLSNTVSAARVPVLADSFANTWVKNAALVFGGALFMVLCQQI
                     SIAVPPSPVPITGGTLGVMLIGSALGSKRGVSAMGLYLLMGLFLPVYSEGREGIDVLV
                     NSASSGYFLGFLVSSYVVGWLAERGGDRKFMTAFISFVFAQLIIFGFGLAGLKLNTGE
                     EWGWVIHNGFTIFIIGGLVKAAIGAAAMPAAWKLSEKTR"
     gene            775..1170
                     /locus_tag="P5138_08650"
     CDS             775..1170
                     /locus_tag="P5138_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRV60687.1"
                     /translation="MLNILATYDLSDALVTTLAIFFFVIWVWIVITILMDLFRDHELN
                     GWAKAAWCLFLIVFPPLAALVYLIFRGHGMRDRAIKEQADAKKQFDDYVRETASTTPV
                     DELHKLDQLRQSGGITDEEYAKMKAKLIN"
     gene            1280..>1846
                     /locus_tag="P5138_08655"
     CDS             1280..>1846
                     /locus_tag="P5138_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60688.1"
                     /translation="MRIHLRLVALIVVTAGLIGVILAMTVFNTDESSNVPDPGPTGET
                     MATGETANTGETVTGVSDGTTLGYAQAIPQPIDGPLLGMNLTAYTPDGYGQASVHKAI
                     RILAGMGSTAITLVPTWYMKSPTANRIAPDKDKSPTDESLAKAIGWILSAGMQVVIKP
                     HVDVVDDSYRGDIQPTNRDQWFRSYGKFI"
BASE COUNT          344 a          578 c          547 g          377 t
ORIGIN      
        1 ggaagaacgg gtgaagccta tctggatagc ttcgcatccg gataggcttc acccgttctt
       61 cctgcctaag aaagggtctc gatgtccacg ctttccaaca cggtgtcagc tgcccgtgtc
      121 cccgttctcg ctgattcgtt cgctaatacc tgggtcaaga atgcggccct ggtgttcggt
      181 ggcgcgctct tcatggtgct gtgccagcag atctcgatcg ctgtgccgcc ctcgccggtg
      241 ccgatcacgg gaggcactct cggcgtgatg ctgatcggtt cggccctcgg ttcaaagcgt
      301 ggcgtctcgg caatgggcct ctacctgctg atgggcctct ttcttccggt ctactcggag
      361 ggacgcgagg gcattgacgt cctcgtgaac tccgcttcga gcggctactt cctcggcttc
      421 ctcgtttcct cctacgtggt cggctggctg gccgaacgcg gcggcgaccg gaagttcatg
      481 accgctttca tctccttcgt cttcgcccag ctgatcatct tcgggttcgg tctcgccggc
      541 ctcaagctga acaccggtga ggaatggggc tgggtcatcc acaacggatt caccatcttc
      601 atcatcggcg gcctggtcaa ggcggcgatc ggcgcggccg caatgcccgc ggcctggaag
      661 ctgtccgaaa agacccgctg aagaactgaa tcatccccaa ggggtgaaag ggggacgggc
      721 cgttcgggtg tagcctgaaa cgctacgtcc cccgtcttcc tgaaaggaac agacttgctg
      781 aacattctgg ccacctacga ccttagtgac gcgctagtca ccacgctggc gatcttcttc
      841 ttcgtgatct gggtctggat cgtcatcacg atcctgatgg acctcttccg tgatcacgag
      901 ctcaacggct gggcgaaggc ggcctggtgc ctcttcctga tcgttttccc gccactggca
      961 gcgctcgtgt atctgatttt ccgcggtcac ggaatgcgag accgggcgat caaggagcag
     1021 gccgacgcca agaaacaatt cgacgactac gtccgtgaaa ccgccagcac cacgccggtc
     1081 gacgagctgc acaagctcga tcagctgcgc cagagcggtg gcatcaccga cgaggagtac
     1141 gccaagatga aggccaagct catcaactga gctttccttt tctgctttcc aggggccggg
     1201 atcgtctgct tatcgggcga taatttcccg gcccctgatc tttttcaagt agggatcggc
     1261 gggggcgtat tgtgaggcgg tgcgcattca ccttcgactc gtcgccctga tcgtcgttac
     1321 ggcaggactg atcggtgtga tcctggcgat gaccgtgttc aacacggacg agagctcgaa
     1381 cgtcccggat cccggaccga ccggcgagac catggccacc ggtgagactg caaacaccgg
     1441 cgagaccgtt accggcgtca gcgacggaac caccctcggc tacgctcagg caatccccca
     1501 gccgattgat ggacccctgc tcggaatgaa cctgaccgcg tacaccccgg acggctacgg
     1561 gcaggcctcg gtccacaagg cgatcaggat cctggccggg atggggagca ccgcgataac
     1621 cctcgtgccg acctggtaca tgaagagccc cactgcgaac cggatcgccc ccgacaagga
     1681 caagtcgccg accgacgaga gcctcgccaa agcgatcggc tggattctct cggccggcat
     1741 gcaggtggtg atcaagcctc atgtcgatgt cgtggacgac tcttacaggg gtgacatcca
     1801 gccaacaaac cgggatcagt ggttccgctc ctacgggaag ttcata
//
LOCUS       DAOTIP010000461         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_74907_length_1845_cov_3.008380, whole genome shotgun sequence.
ACCESSION   DAOTIP010000461 DAOTIP010000000
VERSION     DAOTIP010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74907_length_1845_cov_3.008380"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="P5138_08660"
     CDS             complement(<1..323)
                     /locus_tag="P5138_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRV60689.1"
                     /translation="MKIAMGSDHAGFELKEAVKSQLEAEGHDVEDVGTHSSESTDYPA
                     FAAKAAELVADGNAEKGVIVCGSGVGVSIVANKVDGIRAVNAHDPAEAKMSRRHNDAN
                     VVTLSG"
     gene            418..1200
                     /locus_tag="P5138_08665"
     CDS             418..1200
                     /locus_tag="P5138_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRV60690.1"
                     /translation="MVAVLALIAGAGALAHADFSPDRPNGVKDPCRTPKVAKKLLCPD
                     LRIGKPSEMYLDRELAPGRALLRATNDIRSRGQGPMELRGHRDRPRSMNVTQAIRKRG
                     GGYSFFPTQGRLRYFNVGYQWGGAYWKVRDPLRFELWRVNANEKKVRLVRTGPKQFYC
                     FRDLARTWPAFPRSPDHAVYPACNQNPKVRNVTLGTSVGWSDIYPSTYDQQWIDVTGL
                     RGCFAYVLHLDPGNFLFELNKRNNYAQRIVRLPWRGYARRGC"
     gene            complement(1197..>1845)
                     /locus_tag="P5138_08670"
     CDS             complement(1197..>1845)
                     /locus_tag="P5138_08670"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRV60691.1"
                     /translation="RPDMIGGVKVVATSVNAGDAARQALESCISKGGVALFPADGLYG
                     LACDPLNEKAIRRIHEIKGRDDGKSSAVMYLSPLAMRELIRDLGQRVSEAAAKLLPGP
                     VTLVVPNPRHRYPLACREDQSRLGIRVIDGPLSGVRFPLFQTSANLSGEPAPSVFEGV
                     VPEVREMVDLAIDGGRLTGLPSTVVDLTEYDELGEWEILREGGMSRGDLIAALGR"
BASE COUNT          300 a          624 c          592 g          329 t
ORIGIN      
        1 cctgaaaggg tgaccacgtt ggcgtcgttg tgacgacggc tcatcttcgc ctcggcagga
       61 tcgtgggcgt tgacggcgcg gatcccgtca accttgttgg cgacgatcga gactccgacc
      121 ccggagccgc aaacgatcac tcccttctcg gcgtttccgt cggcaacgag ttccgctgcc
      181 ttcgccgcga aggccgggta gtcggtcgat tcggacgaat gggtaccgac gtcctcgacg
      241 tcatgccctt cggcctcgag ttgtgatttg actgcttcct tcagttcgaa tccggcgtga
      301 tccgatccca tcgcaatttt catgcggcga gcctagcggc gcgcggagcg ggcggactgc
      361 tcctctacca tcgggtgcgt gcggaggcaa ttttcggcca gagttccaga ggtatttgtg
      421 gtcgccgtcc tggcgttgat cgccggggcc ggcgctcttg cgcatgccga tttcagcccc
      481 gaccggccga acggagtcaa ggatccctgc cgcacgccga aggtcgccaa gaagctgctc
      541 tgcccggacc tccggatcgg caagccctcg gagatgtatc tcgaccggga actggccccc
      601 ggacgggccc tgctccgggc gaccaacgac atccgcagcc gcggccaggg gccgatggag
      661 ctccgcggtc accgcgaccg gccccgctcg atgaacgtca cccaggcgat caggaagcgt
      721 ggcggcgggt attcgttctt tccgacccag ggtcgcctgc gctacttcaa cgtcggctac
      781 cagtggggag gcgcctactg gaaggtcagg gatcccctgc ggtttgagct ttggcgggtc
      841 aacgccaacg agaagaaggt caggctggtc cgcacgggcc ccaagcagtt ctactgcttc
      901 cgtgatctgg cccggacctg gccggccttc ccgcgttcac ccgaccacgc ggtctacccg
      961 gcctgtaatc agaacccgaa ggtccgcaac gtgaccctcg gaacctcggt cggctggtcg
     1021 gacatctatc cgtcgaccta tgaccagcag tggatcgacg tgacggggct gcgtggatgt
     1081 ttcgcctacg tcctgcacct ggatccgggc aacttcctct tcgagctgaa caagaggaac
     1141 aactacgccc agcggatcgt ccgactcccc tggcgcggat acgcgcgccg cggctgctag
     1201 cggccgagcg ccgcgatcag gtcgccgcgg ctcattccgc cttcgcggag gatctcccac
     1261 tcgccgagct cgtcgtattc ggtcaggtcg accacggtcg aaggcaggcc ggtcagccgt
     1321 cccccgtcaa tcgccaggtc gaccatctcc ctcacctcgg gcaccacgcc ttcgaagacc
     1381 gaaggagcgg gctcgccgct caggttggca ctcgtctgaa acagcgggaa acgcaccccg
     1441 gaaagcggcc cgtcgatgac cctgattccg agccgggact gatcctcgcg gcaggccagc
     1501 gggtagcggt gccggggatt gggaacgacc agggtcaccg gtccggggag cagcttcgcg
     1561 gccgcttcgc tgacccgctg gccgaggtca cggatcagct cgcgcatggc cagcggcgag
     1621 agatacatca ccgcggatga cttgccgtcg tctcgtccct tgatctcgtg gatccggcga
     1681 atcgccttct cgttgagagg gtcgcaggcg agcccgtaga ggccgtcggc gggaaaaagc
     1741 gcgacgccgc ccttgctgat gcaactctcg agggcctgcc gggcggcatc cccggcgtta
     1801 acgcttgtgg cgacgacttt cactcccccg atcatgtcag gccgg
//
LOCUS       DAOTIP010000462         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_75477_length_1834_cov_4.057898, whole genome shotgun sequence.
ACCESSION   DAOTIP010000462 DAOTIP010000000
VERSION     DAOTIP010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75477_length_1834_cov_4.057898"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="P5138_08675"
     CDS             complement(<1..393)
                     /locus_tag="P5138_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60692.1"
                     /translation="MAVVARVEDTLHDREVRQAGAMGEAGTLRDEEITVSDFTTELSE
                     RIPGVEFELEPEQEMLTVNMGPHHPATHGVLRLVVDLEGEVVKDLKPVIGYVHTGIEK
                     SCETMQYWKVIPFVERMDYLSYFFNMQAY"
     gene            complement(384..932)
                     /locus_tag="P5138_08680"
     CDS             complement(384..932)
                     /locus_tag="P5138_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRV60693.1"
                     /translation="MPDAPGLELILAEINREHPGAVLDTSFDREQAALIIDPAKVVEV
                     LTWLKESPGQEYTFLSSVHGADHFPLTPRFAVHYELLNRERYERLRVKAVIADPAKVM
                     EVDGELLPVVPSVVELFPTADFQEREVYDFFGIVFEGHPDLRRILMPEDYVGWPQRRD
                     FPVGGEPVIFTRDEVTNPGWWQ"
     gene            complement(925..1539)
                     /locus_tag="P5138_08685"
     CDS             complement(925..1539)
                     /locus_tag="P5138_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRV60694.1"
                     /translation="MLRGDLSGEDLDKYVEERVVTTTLEKVLNSARAHSIFPATFGLA
                     CCAIEMMSTVSPRYDIARFGAEAFRASPRQADLLILSGRVSIKMAPVVRRIYDQMLEP
                     KWAISMGACSSSGGMFQNYAVVQGADKFMPVDVHVPGCPPRPEALLYGFTKLQQKIMG
                     NPDPGWRSRYNAHGTEEWARGGPASKPSEEALEAYAKAREVLGA"
     gene            complement(1626..>1834)
                     /locus_tag="P5138_08690"
     CDS             complement(1626..>1834)
                     /locus_tag="P5138_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRV60695.1"
                     /translation="KTFRFGVSFYLVAMLFILFDIEVVFLYPVGAILKSTDSVFVLAE
                     VVLFVALLFVALIYVWRKGALDWR"
BASE COUNT          329 a          568 c          612 g          325 t
ORIGIN      
        1 ataggcctgc atgttgaaga agtaggagag atagtccatc cgctcgacga aggggatgac
       61 cttccagtac tgcatggttt cgcaggactt ctcgatgccg gtgtggacat agccgataac
      121 cggcttcagg tccttgacca cctcgccctc gaggtcgacg accaggcgca gcacgccgtg
      181 ggtcgccggg tgatgcgggc ccatgttgac cgtgagcatc tcctgctccg gctcgagctc
      241 gaactcgact ccggggatcc gctccgacag ctcggtggtg aaatcggaga ccgtgatctc
      301 ttcgtcgcgg agcgtgccgg cctcgcccat ggcgccggcc tgcctgacct cgcggtcatg
      361 gagtgtgtct tcgaccctgg ccactactgc caccagcccg gattggtcac ttcgtcacgg
      421 gtgaagatca ccggttcacc gccgaccggg aagtcgcggc gctgcggcca gccgacgtag
      481 tcctcgggca tcaggatccg gcgcaggtcc gggtggcctt cgaagacgat gccgaagaag
      541 tcgtagacct cgcgctcctg gaagtccgcg gtcggaaaga gttcgaccac cgacgggaca
      601 accggcagca gttctccgtc gacctccatg actttggcgg gatcggcgat cacagctttc
      661 acgcggaggc gttcgtatct ctctctgttc aggagttcgt agtggacagc gaatctcggg
      721 gtcaggggga agtggtcggc tccgtggacc gagctgagga aggtgtactc ctggccgggg
      781 gattccttga gccaggtcag tacctcgacc accttcgccg gatcgatgat cagggccgcc
      841 tgctcccggt cgaagctcgt gtcgagcacg gcccccgggt gttcgcggtt gatctcggcg
      901 aggatcagtt ccagtccggg cgcgtcaggc accgaggacc tcccgggcct tcgcgtaggc
      961 ctcgagtgct tcctcggaag gcttcgacgc cggcccgcca cgggcccact cttccgtgcc
     1021 gtgggcgttg tagcgactcc gccagcccgg atcgggattg cccatgatct tctgctgaag
     1081 cttggtgaag ccgtaaagaa gcgcctcggg acggggcggg cagccgggca catgcacgtc
     1141 caccggcatg aacttgtcgg ctccctggac caccgcgtag ttctggaaca tcccgcccga
     1201 tgaggagcag gcgcccatcg agatcgccca tttcggttcc agcatctggt cgtagatgcg
     1261 gcggacgacc ggggccatct tgatcgagac ccggccggag agaatcagca ggtcggcctg
     1321 gcgcggcgag gcacggaagg cctcggcgcc gaaacgggcg atgtcatagc gcggcgaaac
     1381 cgtcgacatc atttcgatcg cgcaacaggc gagaccgaag gtggcgggga agatcgaatg
     1441 ggcgcgggcg gagttcagga ccttctcgag ggtggtcgtg accacccgct cctcgacgta
     1501 cttgtcgaga tcctcgcccg acaggtcgcc gcgcagcatg tcgcgcgcct tgagctggcg
     1561 ggcccggagc tcctccgggg atttcggttg ctcgaggggg gcgtgaaccc gcaacttctg
     1621 gcgttctatc tccaatccag tgcccccttg cgccagacgt agatcagggc cacgaagagc
     1681 agcgccacga agaggacgac ttcggccagg acgaagaccg agtcggtcga cttgaggatg
     1741 gcccccaccg ggtagaggaa aacgacttcg atgtcgaaca ggatgaacag catcgcgacc
     1801 aggtagaagc tgactccgaa acggaaggtc ttgg
//
LOCUS       DAOTIP010000463         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_75684_length_1831_cov_3.056869, whole genome shotgun sequence.
ACCESSION   DAOTIP010000463 DAOTIP010000000
VERSION     DAOTIP010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75684_length_1831_cov_3.056869"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="P5138_08695"
     CDS             <1..1486
                     /locus_tag="P5138_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRV60696.1"
                     /translation="RLTELMERAGAFFANFFQTADEASKARQYLAGRGLGEEILKEFG
                     VGYAPSAWDTLLVRSQQAGFKVGELERAGLAQKGRNGGFYDRFRERITFPIRDSRGRA
                     VGFGARAMRDEQGAKYINTAENELFHKSEILYGIDRARAAMARADRAILVEGYTDVIA
                     LHQEGLAESVGIMGTALTEQQVAQLSAVVNTVILALDADAAGQKAMLRAQEVAAGRQL
                     QILVAAMPDGIDPAEMVIEEGGADRFRALVESAVDLAEFQVDLILAGVDLDSPRDRDR
                     GLAEASPVLAKLPPGATRQELTRRVGERLQIDSSVVISRVESEEQSGGQTSQPAREAV
                     AGSPGPAVPVSAGPDASPAPPTVLSRRERSERWLLAMCVAKPDEGRQWLEKLDTRHLS
                     SPLIERTVAWLKEHLDDPASGLDPQDRELQKMIAALVARADPDLVGTGSIRRNYMELE
                     LAALEDEISEAAKAGDAARRAELQRHRSELVERLRKAAIDEPAA"
     gene            1522..1594
                     /locus_tag="P5138_08700"
     tRNA            1522..1594
                     /locus_tag="P5138_08700"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Asn,seq:gtt)
     gene            complement(1627..>1831)
                     /locus_tag="P5138_08705"
     CDS             complement(1627..>1831)
                     /locus_tag="P5138_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRV60697.1"
                     /translation="RITWAEVGRSIRWSLTGPRSLRWAVAEILSDPVYRSRAGEVADW
                     QRRHDGPARGAELVEALTASGKS"
BASE COUNT          318 a          598 c          642 g          273 t
ORIGIN      
        1 caggctgacc gaactgatgg aacgggccgg cgcgtttttc gcgaacttct tccagaccgc
       61 cgatgaggca tcgaaggccc ggcagtacct ggccggtcgc ggtctcgggg aggagatcct
      121 caaggagttc ggtgtcggct acgcgcccag cgcctgggac acgctgctgg tccgcagtca
      181 gcaggccggt ttcaaggtgg gggagcttga gcgggccggg ctcgcgcaaa aggggagaaa
      241 cggtggcttt tacgaccggt tccgggaacg catcaccttt ccgatccgcg attcccgcgg
      301 tcgggcggtc ggcttcggtg cccgggcgat gcgtgacgaa cagggtgcga agtacataaa
      361 cacagccgag aacgagctgt tccacaagag cgagatcctc tacgggatcg accgggccag
      421 ggccgcgatg gccagggccg accgggcaat cctcgtcgag ggatacacgg atgtgatcgc
      481 cctgcatcag gaaggcctgg ccgaatcggt cgggatcatg ggcaccgccc tgaccgaaca
      541 gcaggtcgcc cagctctccg cggtcgtgaa cacggtgatc ctggcgcttg acgctgacgc
      601 ggccggccag aaggcgatgc tgcgcgcgca ggaggtggcg gctggtcggc aactccagat
      661 tctcgttgcc gcgatgccgg acgggatcga tccggccgag atggtgatcg aggaaggtgg
      721 cgccgacagg ttccgggcac tggtcgagag tgcggtcgac ctggccgagt ttcaggtcga
      781 cctgatcctc gccggggttg accttgattc accgcgcgac cgcgaccgcg ggctggccga
      841 ggcgtcaccg gtgctggcca agctgccacc gggggcgacc aggcaggaac tgacccggag
      901 ggtcggggag cgtctccaga tcgattcgag cgtggtgatc tcccgagtcg aatccgagga
      961 gcagtcgggc ggccagacct cacaaccggc cagggaagca gttgcgggct ctcccggtcc
     1021 ggccgtgccg gtcagcgccg ggccggacgc ttcacccgcc ccgccaaccg tgctctcccg
     1081 ccgcgaacgc agcgaaaggt ggcttctggc catgtgtgtc gcgaagcccg acgagggccg
     1141 tcagtggctg gagaagctgg atacccgtca tctgagttcg cccctgatcg agcgcacggt
     1201 ggcctggctg aaggagcacc tcgacgaccc cgcctccggc ctcgatccgc aggaccgcga
     1261 gctgcagaag atgatcgcgg cactcgtcgc ccgggccgac cctgacctgg tcggaaccgg
     1321 atcgatcagg cgcaactaca tggagctgga actcgcggca ctggaagacg agatttccga
     1381 ggcggcgaag gcaggtgacg ccgccagacg ggccgagctc cagcgccatc gttccgaact
     1441 ggtcgagcgg ttgcggaagg ccgcaatcga cgaaccagcc gcctgaaacc cggccccatt
     1501 cggtagtctg atcgtccgca gtcgggggta gctcaatcgg tagagcattc ggctgttaac
     1561 cgaacggttg tcggttcgag tccgaccccc cgagtcgccc gagcccgccc ggaatccggc
     1621 gcgggctcaa ctttttccag aggccgtcag ggcttcgacc agttcggccc cccgggccgg
     1681 accgtcgtgc cgacgctgcc agtcagcgac ctccccggcc cgcgaacggt agacgggatc
     1741 cgacaggatc tcggccaccg cccagcggag cgagcggggc ccggtcaggc tccagcggat
     1801 cgaacgcccg acctccgccc aggtgatccg c
//
LOCUS       DAOTIP010000464         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76036_length_1825_cov_3.562147, whole genome shotgun sequence.
ACCESSION   DAOTIP010000464 DAOTIP010000000
VERSION     DAOTIP010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76036_length_1825_cov_3.562147"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="P5138_08710"
     CDS             complement(<1..223)
                     /locus_tag="P5138_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60698.1"
                     /translation="MNRFRTKVAAALVAAGALITVAVAQAATSDTLGVTTVTQRIVPD
                     SSSGFNFLTTGPGEDYTVRDGSEDGGTALG"
     gene            complement(359..1783)
                     /locus_tag="P5138_08715"
     CDS             complement(359..1783)
                     /locus_tag="P5138_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV60699.1"
                     /translation="MDHKLLLAGEWIETGDWDEVLSPWDESLIARVAQGDAETVDKAV
                     RAAHQVFTDSPIPQYERAEILDKAAHLIRERQEDLAQTISAEAGKPIKTARVEASRAA
                     DTMTFSAVEARKLTGGTVPMEASTAGIGKLGVMLRVPLGVVGAISPFNFPLNLVCHKL
                     GPALAAGNAVVLKPAGQTPISSIKLAEVLIDAGLPAGWLSVVPGSGSSVGNAIVEHEL
                     TAAISFTGSAPVGWGIRSKVPHKKVGLELGSNAPLIVNEDGDWETAADKAQIHAFSHA
                     GQSCISIQRILVHEAVADKFIERLVSNMENLKVGNPAEDDTDVGPLITPGDRDRVKGW
                     IDEAVADGAKVLTGGELVDEGRCLAPTLIEAPSREAKVWCEEIFGPVATVDRFGDFDE
                     GLARANDSKFGLQAGVFTRDMGNGLKAAETLEFGGVLINEVPTFRADQQPYGGVKDSG
                     NTREGPAYSIIEMTEERFITLQGG"
BASE COUNT          285 a          600 c          605 g          335 t
ORIGIN      
        1 tgccgagtgc ggtgccgcca tcctcggagc cgtcccgaac ggtgtaatcc tcgccggggc
       61 cggtggtcag gaagttgaag ccggacgagg agtcgggcac gatccgctgg gtcacggtcg
      121 tgactccgag cgtgtcgctg gttgccgcct gcgcgacagc cacggtgatc agcgcgcccg
      181 ctgcaaccag tgcggcagcg actttggtcc tgaaccggtt catgaattct ccttgcggcc
      241 ggttccccga accggcacct gtggtgtgga aagctcgtcc ggtctcctcc ggacgtggac
      301 ggaatgagcc tacgttaccg ccatgtaaag aaaccggcca gcggccccct tgttgcgctc
      361 aaccgccctg gagggtgatg aagcgttcct cggtcatttc gatgatcgag taggcaggac
      421 cttcccgggt gttgccggag tccttgacgc cgccgtaagg ctgctggtcc gcccggaagg
      481 tcggcacctc gttgatcagg acgccgccga actcgagcgt ttcggccgcc ttgagcccgt
      541 tgcccatgtc gcgggtgaac acgccggcct gcaggccgaa cttggagtcg ttggcccggg
      601 cgagcccttc gtcgaagtcg ccgaaacggt cgaccgtagc taccggaccg aaaatctcct
      661 cacaccacac cttcgcctca cgggaagggg cctcaatcag ggtgggggcc aggcagcggc
      721 cctcgtcgac cagctcgcca ccggtcaaga ccttcgcgcc gtcggcgacg gcctcgtcga
      781 tccatccctt gacccggtcc cggtcgccgg gagtgatcag cgggccgacg tcagtgtcgt
      841 cctcggccgg gttgccgacc ttgaggtttt ccatgttgga aaccagccgt tcgatgaact
      901 tgtcggcgac cgcttcgtgg acgaggatcc gctgaatgga gatgcaggac tgcccggcgt
      961 gggagaaggc atggatctgg gccttgtcgg cggcggtctc ccagtcgccg tcctcgttga
     1021 cgatcagcgg cgcgttcgaa ccgagttcga ggccgacctt cttgtgcggg accttgctgc
     1081 gaatgcccca tccgaccggg gccgagccgg tgaaggaaat cgccgcggtg agttcgtgtt
     1141 caacgatcgc gttgcctacc gaagagccac tgccggggac cacgctcagc cagcctgccg
     1201 gcaggcccgc gtcgatcagg acctcggcaa gcttgatcga ggagatcggg gtctggccgg
     1261 cgggcttcag cacgaccgcg ttgccggccg cgagggccgg accgagtttg tggcagacca
     1321 ggttgagcgg aaagttgaat gggctgatcg caccgaccac tccgagcggc accctcagca
     1381 tcacgccgag cttgccgatc ccggccgtgg acgcttccat cggcacggtg cccccggtca
     1441 gcttgcgtgc ctcgaccgcg gaaaaggtca tcgtgtcggc agcacgggag gcctcgaccc
     1501 gggcggtctt gatcggcttg ccggcttcgg cggaaatcgt ctgggccagg tcttcctgcc
     1561 gctcccggat cagatgggcc gccttgtcga ggatctcggc ccgctcgtac tgggggatcg
     1621 gcgaatcggt gaaaacctgg tgggcggccc tgaccgcctt gtccacggtc tcggcatcac
     1681 cctgcgcgac ccgggcaatc aggctttcgt cccagggact caatacctcg tcccagtcgc
     1741 ccgtttcgat ccattcacct gccagaagca acttgtggtc caacgcggat tctcctcgga
     1801 tgtggctgtc gggaccggtc cggcc
//
LOCUS       DAOTIP010000465         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76181_length_1823_cov_2.274321, whole genome shotgun sequence.
ACCESSION   DAOTIP010000465 DAOTIP010000000
VERSION     DAOTIP010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76181_length_1823_cov_2.274321"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1355
                     /locus_tag="P5138_08720"
     CDS             171..1355
                     /locus_tag="P5138_08720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60700.1"
                     /translation="MIAVAFAVFAILFAVLPSPARSANPYTVTSDIPYGPANDQSGSN
                     LLDIYVPNADSDGPRPVFVWIHGGGWFTGDKTRSTMAYKAKALVRAGFVFVSINYRLS
                     PELSGPKALSKNRLRYPTHHLDAARALGWVNRHIADYGGNPKRMVIGGDSAGGQISSL
                     LATKPAFLKARGMSTRQIKGVLALDAVGYDVPRMMTKAYRRINAGFQKMMFNAFGTPA
                     EEKKSPHWNAASPVRFADRSDPPIFYVVPTTAPDRWVDAHKMARLLGQRLGLVSHRVR
                     TEHAGVVPLLGNPNGDMGVTAPLMRFAKAAVNPVKYRLVVRGTRVVRGTGKEKLGRIA
                     LRIASRPAARLITCRVDRGRETLCPRFWKLKPGLHTLRVKAYGNTGRADGTAFVKLRV
                     TG"
     gene            1352..>1823
                     /locus_tag="P5138_08725"
     CDS             1352..>1823
                     /locus_tag="P5138_08725"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRV60701.1"
                     /translation="MKRITATGLAVAAVVIALAGSGLSAQASARTYVPSFSAVKKDKG
                     PVFRQRCLVFTNRVTSPPCHYGVVGSEKKVFVLGDSHALQWTPALIGIAERRGWELTT
                     LLRKNCTAALVNISPVCNRWRQNALKRIRQEKPRMVFVASNTAANTYVVRKGMHL"
BASE COUNT          313 a          602 c          574 g          334 t
ORIGIN      
        1 atcgcctgat catgccgctg ctgctgctgc acggcaccgg cgatcgcctg accggcagtc
       61 aggtttttcg cgctctggcc gcaccccggc cgccgaaagg ttgttaacta ccccgcatgc
      121 tccagccggg gaactcagtc aatggtcggg cgcgcctgct gctggccggt ttgatcgcag
      181 tcgcgttcgc tgtcttcgcg atcctctttg cggtcctgcc ctcgccagca cgctcggcta
      241 acccgtacac ggttacttcc gacatcccgt acggtcccgc gaacgatcag tccgggtcca
      301 atctcctcga catctacgtg ccgaatgccg attccgacgg gccgcgaccc gttttcgtgt
      361 ggatccacgg tggcggctgg ttcaccgggg acaagaccag gtcgaccatg gcctacaagg
      421 ccaaggctct cgtcagggcc ggattcgtct tcgtctcgat caactaccgg ctttcgcccg
      481 agttgtccgg ccccaaggca ctttcgaaga accggctccg ctatccgacc catcatctgg
      541 atgcggcccg ggcacttggc tgggtgaaca ggcacatcgc tgactacggc ggcaacccga
      601 agcggatggt gatcggcggc gattcggccg gcggccagat ctcctccctg ctcgccacca
      661 agccggcctt cctcaaggcg aggggcatgt cgacccgtca gatcaagggc gtcctcgctt
      721 tggacgccgt cggctatgac gttccccgga tgatgaccaa ggcctaccgc aggatcaacg
      781 ccggattcca gaagatgatg ttcaacgcct tcggcacccc ggcggaagag aagaagtctc
      841 cgcattggaa cgcggcttca ccggtgcgtt tcgccgatcg ctccgacccg ccgatcttct
      901 acgtggtgcc gaccaccgca cccgaccgct gggtggacgc ccacaagatg gcgaggctcc
      961 tcggtcagcg gctcgggctc gtttcccatc gggtcaggac cgagcacgcc ggggtggttc
     1021 cgttgctcgg taatccgaat ggcgatatgg gggtgacggc cccgctgatg cgcttcgcga
     1081 aagcggcggt gaacccggtg aaataccggc tggttgtcag gggaacccgg gtcgtccggg
     1141 gaaccgggaa ggaaaagctc ggcaggatcg cgctccgtat tgcttcacgc cccgcggcga
     1201 ggctgatcac atgtcgtgtc gatcgcggcc gcgagacgct ctgtccgcgg ttctggaagc
     1261 tgaagccggg cctgcacacc cttcgggtga aggcttacgg aaacacgggc cgggcagacg
     1321 gcacggcctt cgtcaagctg agggtgaccg gatgaaacga attaccgcca ccggcctggc
     1381 cgtcgcggcg gtcgtcatcg ctctcgctgg atcgggtctc tccgcccagg cttcggccag
     1441 aacctacgtg ccctcgtttt ccgcggtcaa gaaggacaag ggaccggtct tccggcagcg
     1501 ctgcctggtc ttcacgaacc gcgttacgtc cccgccgtgc cattacgggg ttgtcggttc
     1561 cgaaaagaag gtcttcgtgc ttggggattc ccacgccctg cagtggactc cggccctgat
     1621 cggcatcgcc gagagaaggg gctgggaact gaccacgctt ctgcggaaga actgcaccgc
     1681 ggcgttggtc aacatcagcc cggtctgcaa ccgctggcga cagaacgcac tcaaacggat
     1741 ccggcaggag aagccgcgga tggtcttcgt ggccagcaat accgcggcga acacctacgt
     1801 ggtccggaag ggcatgcacc tga
//
LOCUS       DAOTIP010000466         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76432_length_1819_cov_2.865646, whole genome shotgun sequence.
ACCESSION   DAOTIP010000466 DAOTIP010000000
VERSION     DAOTIP010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76432_length_1819_cov_2.865646"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..380)
                     /locus_tag="P5138_08730"
     CDS             complement(33..380)
                     /locus_tag="P5138_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRV60702.1"
                     /translation="MNFRPKNEHELARLKDEELIAYAVAARDADRPEDFALAVGIFIL
                     GRERMVRAMVADKVPTQSVEDVVQEVLVSAIESLSRINGTVTGQFVNWLKQITAFRIA
                     DYHKSAGKHAGMR"
     gene            647..>1819
                     /locus_tag="P5138_08735"
     CDS             647..>1819
                     /locus_tag="P5138_08735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60703.1"
                     /translation="MVVVGGGLTGLWAAVRAKEREPGRDVVLIERESLAIGASGRNGG
                     FMSSSLVHGLGNGLSRFPDEMPLLEKLGFRNLDEIEKVIEAQAIDCDLRKPGVIEVAV
                     TDAQMVELEEGVEEFARYGHEAVMLDAGQIRAEVNSPIYRGGMWQKSGEWTVDPVRLC
                     DGLADYASRLGVRIYELTPMKGFEKSGAGVRVRTPTGSIQAARVLLATSAFKVPVGQI
                     RRRVIPVYDYVLVTEPLTRSQWDSLGWRNYQGLSDSSNQFHYYRPIDGGPDAATDEKP
                     GGRLLWGGYDAIYNYGGRVDEEAYQRDSSFAGLAQRFFTAFPQLEGVSFSHRWGGAID
                     TCSRFFAFYGTTMEGRVGWAVGHTGLGVGASRFSANLGLDLLEGIEGVWPLRAELGR"
BASE COUNT          304 a          580 c          605 g          330 t
ORIGIN      
        1 taccggacag ttcgtcaact ggctctagca gatcaccgca ttccggcatg tttcccggca
       61 ctcttgtggt agtcggcaat ccggaatgcg gtgatctgct tgagccagtt gacgaactgt
      121 ccggtaacgg ttccgttgat ccgggaaagc gattcgatcg cgctgaccag cacttcctgg
      181 accacgtcct cgaccgactg cgtgggcacc ttgtcggcga ccatcgcccg caccatccgt
      241 tcgcggccga ggatgaatat cccgaccgcg agcgcgaaat cttcgggccg gtcggcatcc
      301 cgggcggcaa ccgcgtaggc gatgagctct tcgtctttaa gtctcgccag ttcatgttcg
      361 ttcttgggcc tgaagttcaa gcttgtcccg ggggtcgtcg cctgcggatt cgttctgaca
      421 gcgccctgaa ttcctgttcc aggccccagg gactcggggc caagataccg cctcggtcgg
      481 ctacagtccg cccatcgcac ccgcgggcac caaagcagtc tggccagata cggagccgtc
      541 ccaatccgat cgtctctcgt atgccgaggc ggaaccttcc cccttctggc tctccggtcc
      601 gaggccggaa ctgcataccc cccttcagga gagcaccgaa gccgatctgg tcgttgtcgg
      661 aggcgggctg accggactct gggcggcggt ccgggcgaag gagcgcgagc ccggacggga
      721 tgtggtcctg atcgagcgcg aaagcctcgc gatcggcgcc agtggccgta acggcggctt
      781 catgtcctca tccctcgtac acggactggg caacggcctg tcccgctttc ccgacgagat
      841 gccgctgctc gagaagctcg gcttccggaa cctggacgag atcgagaagg tgatcgaagc
      901 gcaggcaatc gattgcgacc tgcgcaagcc cggcgtgatc gaggtcgcgg tgaccgacgc
      961 ccagatggtg gagctcgagg agggagtcga ggagttcgcc aggtacgggc atgaggcggt
     1021 gatgctggac gccggccaga tccgggccga ggtgaactcg ccgatctaca ggggcggcat
     1081 gtggcagaag tccggcgaat ggacggttga cccggtccgg ctctgcgacg ggctcgccga
     1141 ctatgccagc cgtcttggag tgaggattta cgagttgacc ccgatgaagg gattcgagaa
     1201 gtccggggcc ggggtcaggg ttcgcacccc gaccggatcg atccaggcgg ccagggtgct
     1261 gcttgccaca tcggcgttca aggtgccggt cggccagatc cgccgcaggg tcatcccggt
     1321 ctatgactac gtgctggtca ccgaaccgct cacccgctcg caatgggact cgctcggctg
     1381 gcgtaactat cagggattgt ccgacagctc gaaccagttc cactactacc gcccgatcga
     1441 cgggggcccg gacgcggcca ccgacgagaa gcccggcggc cggctgctct ggggtggcta
     1501 cgacgcgatc tacaactacg gcggccgggt ggatgaggag gcctatcagc gcgacagcag
     1561 cttcgcgggt ctcgcccagc gcttcttcac cgccttcccc cagctcgaag gtgtgagctt
     1621 ctctcaccgt tggggagggg cgatcgacac ctgctcacgg tttttcgcct tctacggaac
     1681 gaccatggaa ggccgggtcg gttgggcggt cgggcacacg ggcctcggag tcggggccag
     1741 ccgcttctcg gcgaacctgg gactcgatct gctcgaaggg atcgagggcg tctggcccct
     1801 gcgcgccgag cttggccgc
//
LOCUS       DAOTIP010000467         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76498_length_1818_cov_2.515031, whole genome shotgun sequence.
ACCESSION   DAOTIP010000467 DAOTIP010000000
VERSION     DAOTIP010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76498_length_1818_cov_2.515031"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1818)
                     /locus_tag="P5138_08740"
     CDS             complement(<1..>1818)
                     /locus_tag="P5138_08740"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRV60704.1"
                     /translation="AALLIASLAVRSVGLVDAAQTATAGLITLIPEGLVLLMSVTFAV
                     AAVKMARRATLVQQMSATESLASVDTICVDKTGTLTDGDLTVVEVIPARVTDSKLAAR
                     ELGRYAASAGDRNLTLEAIAEEFPGDAEPVTGEVAFSSEWKWSALTFDEDGARYSLVL
                     GAPDLMIDAGALRLEEHLSARLAEETAAGRRVVALNRSTSPLPASGIDGYPAGMEPVA
                     LVVLRENLRPGVEETLELMRSEDVALKLISGDARATVTAVAAAVGIPADAGVIEGPDL
                     PEDEEALAEVVASNTIFCRIRPDQKRQLVSALAESGAYTAMIGDGVNDVPALKSARMA
                     VAMGSGSQVTKSVADVVLLEDQFSRLPEAIAEGRRIARNIHRLGRLYLTKTVYAAALI
                     VLVSVPGFTFPFLPRHLTLAAFLTIGIPSFVLALAPSDGPLYRGRLLRALAAFAVPAG
                     LATALSSILSYFLVDVVFGGNVEEGRTAATTTLIILGLCFVLLLERGPGREHIAIQSY
                     MLAMIAGLGALFAGILAVPQLREFFDMAMLGAEQWFISMLAAALGLVFASLMWRLPQI
                     QAWEVPDESAFPPLERVPPPENAPPQRPTAGPIHRETTPV"
BASE COUNT          296 a          608 c          643 g          271 t
ORIGIN      
        1 accggagtcg tctctctgtg gattgggcca gcagtgggcc tttggggcgg cgcgttctcg
       61 ggcggcggca cccgctccag cggcgggaag gcggattcgt ccgggacctc ccaggcctgg
      121 atctgcggca gacgccacat caggctggcg aaaacgaggc cgagggcggc ggcgagcatc
      181 gagatgaacc actgctcggc ccccagcatt gccatgtcga agaactcccg cagctgcggc
      241 accgcgagga tcccggcaaa cagtgcgccg aggccggcga tcatcgccag catgtagctc
      301 tggatcgcga tgtgctccct tccgggtcca cgctccagca gcagcacgaa gcagaggccg
      361 aggatgatca gcgtcgtggt cgcggcagtg cggccctctt ccacgttgcc gccgaagacc
      421 acgtcgacca ggaagtagga gaggatcgat gacagtgcgg tggccagccc ggccggcacc
      481 gcgaaggcgg ccagcgccct gagcagccgg ccccggtaga gaggcccgtc ggatggggcc
      541 agggcgagca cgaaggaggg aatgccgatg gtcaggaagg ccgccagggt caggtgccgg
      601 gggaggaagg gaaaggtgaa cccgggtacc gagaccagca cgatcagggc ggctgcgtag
      661 acggtcttgg tcaggtagag ccggccgagc cggtggatgt tgcgggcgat cctccggcct
      721 tcggcgatcg cttccgggag gcggctgaat tgatcctcga gcaggacgac gtcggccacc
      781 gactttgtga cctggctgcc cgatcccatc gccacggcca tgcgggcact cttcagggca
      841 ggcacgtcat tgacgccgtc accgatcatc gcggtatagg cgcccgattc cgccagggcc
      901 gagaccagct gtcgcttctg gtccggcctg atccggcaga agatggtgtt ggaggcaacc
      961 acctcggcca gcgcttcctc gtcctcgggc aggtcggggc cctcgatcac ccctgcgtcg
     1021 gccggaatgc cgacggccgc tgccaccgcg gtgaccgtgg cccgggcatc gccggagatc
     1081 agcttcaggg cgacgtcctc gcttcgcatc agctccagcg tctcctcgac cccgggtctg
     1141 aggttctccc tcagcaccac cagggcgacc ggctccatac cggccgggta tccgtcgatg
     1201 ccgctggccg ggaggggcga ggtggagcgg ttcagggcga ccacccgccg gccggccgcc
     1261 gtctcttcgg ccagacgggc ggaaaggtgc tcctcgagcc tgagggcacc ggcgtcgatc
     1321 atcaggtcgg gcgcgccgag gacaaggctg taacgggcgc cgtcctcgtc gaaggtcagg
     1381 gcgctccact tccactcgga ggagaacgcg acttcgccgg tcaccggctc cgcatcgccg
     1441 gggaactcct cggcgatcgc ctcgagggtc aggttccggt cgccggcgct tgccgcatat
     1501 cgaccgagct cgcgggcggc gagctttgaa tcggtcaccc tggccggaat gacttcgacc
     1561 acggtcaggt cgccgtcggt cagggtgccg gtcttgtcta cacagatcgt gtcgaccgaa
     1621 gcgagcgatt cggtggcgct catctgctgg accagggtcg cccgcctggc catcttcacc
     1681 gcggcgaccg cgaaggtcac gctcatcagc aggaccagcc cttccgggat cagcgtgatc
     1741 aggcccgcgg tcgcggtctg ggccgcgtcg accaggccga cacttcgcac ggccagcgaa
     1801 gcgatgagca gagccgcc
//
LOCUS       DAOTIP010000468         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76668_length_1815_cov_3.512500, whole genome shotgun sequence.
ACCESSION   DAOTIP010000468 DAOTIP010000000
VERSION     DAOTIP010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76668_length_1815_cov_3.512500"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="P5138_08745"
     CDS             <1..630
                     /locus_tag="P5138_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60705.1"
                     /translation="ALVPKDVMRNTAFSAACVAILFVSATFFSVLFYVPQYLQKAGDL
                     SAFQSGLGLLPFMAVFALTSFVAGPLYNRVGGRIIVTLGAAGIALGPFLISFGVESSG
                     SLGGIIPGLVVLGIGVGLFYSSATTIGVTAVDESRSSLAGGIIYMFQVAGGSVGLALT
                     TTLFASASGLISGLHVAFRFNAIAAAVGFLIVLFVVGPRVQPVKKQTSA"
     gene            671..>1815
                     /locus_tag="P5138_08750"
     CDS             671..>1815
                     /locus_tag="P5138_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60706.1"
                     /translation="MGFRRISSAFVCSLAAVLGLALPTGASAGLDDRDSEPVVLTGAD
                     TPLLTGADPQAVVAFSWFGSWQQVPVQVDERKMIDYRPVRQLSFNSTNEFRAMAYADP
                     DTWAEADGVPQTVTTPGDRGSGAVIPGTTGDPTLDENDEIAMMSEDAGGSAAGKKAPD
                     GVDPATLTPVKVTDPLDPGQSRYIYLFLTDDGLDPDANSDYVSYDQVYSPALAGGYRD
                     GYDFGKLGDDVNGPPINPEDSLITTSRYEIGIPGRWMIDRLVIAAGEGDTDILDGDKS
                     TVSPNGCSRNELTFSRGGGGFIANIDGPVRAIRSFIGANSGTFTQREYVFYEGMFENR
                     TFLRVHPGINQFVTAMDLSADAVGMTYRNELNPDGVPIDGVPDAPVAG"
BASE COUNT          290 a          627 c          563 g          335 t
ORIGIN      
        1 gccctggtgc ccaaggacgt gatgcggaac acggctttca gcgccgcctg cgtggcgatc
       61 ctcttcgtct cggcgacgtt tttctcggtc ctcttctacg tgccccagta cctgcagaag
      121 gcgggtgacc tctccgcctt ccaatccggg ctcggactcc tgcccttcat ggccgtgttc
      181 gccctcacct cgttcgtggc cggaccgctc tacaaccggg taggtggccg gattatcgtg
      241 accctgggag ctgccggcat agcactcggt ccgttcctta tctccttcgg tgtcgagtcg
      301 agcggttcgc tcggcgggat cattcccggc ctggtcgtgc tgggtatcgg ggtcgggctt
      361 ttctattcgt cggcgaccac gatcggagtg accgcggtgg atgaatcgag gtcgagcctg
      421 gccggcggga tcatctacat gttccaggtc gccggtggct cggtcgggct ggccctgacc
      481 accaccctgt tcgcctcggc cagcgggctg atcagcggac ttcacgtggc cttccgtttc
      541 aacgcgatcg ccgccgcggt cgggttcctg atcgttctgt tcgtggtcgg tccgcgagta
      601 cagccggtca aaaagcagac atcggcctga tccccgcagc gaacggggtc tgcttgggtt
      661 aacggcaaca atgggtttcc gcagaatttc ctccgcgttc gtctgttccc tcgccgcagt
      721 cctcggcctc gccctcccga ccggcgcttc agccggcctc gacgaccggg actcggagcc
      781 ggtcgtgctc accggagccg acacgccgct cctgaccggt gccgaccccc aggcggtcgt
      841 ggccttttcc tggttcggtt cctggcagca ggttccggtc caggtagacg aacgcaagat
      901 gatcgactac cggccggtca ggcagctctc cttcaactcg accaacgagt tcagggcgat
      961 ggcctacgcg gacccggaca cctgggccga agccgacggc gtgccccaga ccgtgaccac
     1021 gcccggcgac cgcggcagcg gcgcggtcat tcccggcacc accggtgatc cgaccctcga
     1081 cgagaacgac gagatcgcga tgatgagcga ggacgccgga ggttccgcgg ctggcaagaa
     1141 ggcgcccgac ggcgtcgatc cggccaccct gacccccgtg aaggtcaccg atccgctgga
     1201 tcccggccag tcccgttaca tctacctctt cctgaccgac gacggactcg acccggatgc
     1261 caattccgac tacgtcagct atgaccaggt ctattcaccg gcgctggccg gcggttaccg
     1321 ggacggctac gacttcggaa agctcggcga cgacgtcaac gggccgccga tcaatcctga
     1381 ggattccctg atcaccacct cccgttacga gatcgggata ccgggccgct ggatgatcga
     1441 ccggctcgta atcgccgccg gtgaaggcga caccgacatt ctcgacggcg acaagtcgac
     1501 cgtttcgccg aacggctgca gtcgcaacga actcaccttc agccgcggag gcgggggatt
     1561 catcgccaac atcgacggtc cggtccgggc gatccgctcg ttcatcgggg ccaacagcgg
     1621 aaccttcacc cagcgcgaat acgtcttcta cgaagggatg ttcgagaacc ggaccttcct
     1681 ccgggtccac ccgggcatca accagttcgt gaccgcgatg gacctttcgg ccgacgcggt
     1741 cggcatgacc taccggaacg agctcaaccc ggacggggtg ccgatcgacg gggtgcctga
     1801 cgcgccggtc gccgg
//
LOCUS       DAOTIP010000469         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76821_length_1813_cov_2.604664, whole genome shotgun sequence.
ACCESSION   DAOTIP010000469 DAOTIP010000000
VERSION     DAOTIP010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76821_length_1813_cov_2.604664"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="P5138_08755"
     CDS             <1..1445
                     /locus_tag="P5138_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV60707.1"
                     /translation="IEASRISEQGRRVAVRLEELRLEMAPLTLDVGESGSASERARRL
                     VDELGEVDAGLATAAEGLRQARAEGPESKRLKALADVHKGAERATTHARRAESLLGER
                     HRERLGEKMAEVENLATLIGALEGSAGETGQAIRERVSRAERTVIGDQGDADEISAEL
                     KACSDLEIEIQGKLRAVAEQVTGAEVEAAHLGDRRAEAAGELARISEKLEIEIPEPEE
                     ELDDEEREQIERRLSNLRTRRERLGPVNPLAKKEYEEALEHVEQLVAQREDTERAMRE
                     LEGVIRDIDAEIKRSFDETFEATATNFEEMIAQLFPGGSGRLRSVDLRPVPKPAAEGE
                     EAESGEEEVEEEDGPRTPNESDFGVELEVTPQGKRMRQMSLLSGGEKAMTALAFVFAV
                     FLARPCPFYILDEVEAALDDANLNRFLELVRRFSDRTQFVIVTHQKLTMDAADVLYGV
                     SMGGDGVTKVISRRLPKGVTALEGTQLEAA"
     gene            1457..>1813
                     /locus_tag="P5138_08760"
     CDS             1457..>1813
                     /locus_tag="P5138_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV60708.1"
                     /translation="MTHWKKLNFADVEDVSPDGVSIQWHFARKALGSPELGVSRFTYE
                     PGARMPWGHRHNSQEEIYVVVSGGGRAKLEEEIIELEPWDVIRVGPSVARSMEAGPDG
                     MEVICIGGHKAEGQDGI"
BASE COUNT          354 a          559 c          643 g          257 t
ORIGIN      
        1 cgatcgaagc ttcaaggatt tcagagcagg gcaggcgggt tgcggtccgg ctcgaggaac
       61 tgcggctgga gatggccccg cttaccctcg atgtggggga aagcggcagc gcaagcgagc
      121 gggccaggcg cctggtcgac gagctcggcg aggtggatgc cgggctcgcc accgcggccg
      181 agggcctgag gcaggcccgg gcggaagggc cggagtccaa acggctgaag gcgctggccg
      241 acgtccacaa gggcgccgag cgcgcgacca ctcacgcccg ccgggccgaa agcctgctcg
      301 gtgagcgcca tcgcgagcgg ctcggagaga agatggccga ggtcgagaac ctcgccacgc
      361 tgatcggagc gctcgaaggt tcggccggtg agaccggaca ggctatccgc gaacgggttt
      421 cccgggccga gcggaccgtg atcggcgacc agggggacgc cgacgagatt tccgccgagc
      481 tcaaggcctg ctccgacctg gagatcgaga ttcagggcaa gttgcgcgct gtggccgagc
      541 aggtcaccgg agcggaggtc gaggcagccc acctcggtga ccgccgggcc gaagccgccg
      601 gcgaactggc ccggatctcg gagaagctgg aaatcgagat cccggagccg gaagaggaac
      661 tcgacgacga ggagcgcgag cagatcgaaa ggcggctgtc caacctgcgg acccgtcgcg
      721 agaggcttgg accggtcaac ccgctcgcaa agaaggaata cgaagaggct ctcgagcacg
      781 ttgagcagtt ggtcgcccag cgggaggaca ccgaaagggc gatgcgcgaa ctcgagggcg
      841 tgatccggga catcgacgcc gagatcaagc gctccttcga cgagaccttc gaggcgacgg
      901 cgacaaactt cgaggagatg atcgcccagc tcttcccggg cggttcaggc cggctccgct
      961 cggtcgatct tcgtccggtg cccaagccgg cagctgaagg cgaggaggcc gaatccgggg
     1021 aggaagaggt cgaagaagaa gacggtcccc ggaccccgaa tgaatcggac ttcggggtcg
     1081 agctcgaggt gacgccccag ggcaagcgga tgaggcagat gagcctgctg tccggcggcg
     1141 aaaaggcgat gaccgccctg gccttcgtct tcgcggtgtt tctcgcccgc ccgtgcccct
     1201 tctacatcct cgacgaggtc gaggcggctc tcgacgacgc caacctcaac cgcttcctcg
     1261 aactggtccg acgcttttca gaccgcaccc agttcgtgat cgtcacccac cagaagttga
     1321 ccatggacgc ggccgatgtg ctttacggag tcagcatggg cggcgacggg gtcaccaagg
     1381 tgatctcgcg tcgcctcccg aagggcgtga ccgccctcga gggcacccag ctcgaagcgg
     1441 cctgaaacgg gccttcttga cgcactggaa gaagctcaac ttcgccgacg ttgaagatgt
     1501 cagccccgat ggtgtttcca tccagtggca cttcgcccgc aaggctctcg gctcgccgga
     1561 gttgggggtg agccggttca catatgaacc gggcgcccgc atgccatggg gacaccggca
     1621 caattcgcag gaagagatct acgtcgtggt ctccggcggc ggtcgggcca agctcgagga
     1681 agagatcatc gagctcgaac cctgggatgt gatcagggtc ggaccttcgg tcgcccgttc
     1741 catggaggcc ggccccgacg gcatggaagt gatctgcatc ggcggtcaca aagccgaagg
     1801 ccaggacggg atc
//
LOCUS       DAOTIP010000470         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_76984_length_1810_cov_3.548148, whole genome shotgun sequence.
ACCESSION   DAOTIP010000470 DAOTIP010000000
VERSION     DAOTIP010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76984_length_1810_cov_3.548148"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="P5138_08765"
     CDS             complement(<1..517)
                     /locus_tag="P5138_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV60709.1"
                     /translation="MSGQKQDTDTGKKPKVVVVMPARQAAQTLEATFAEIPKDAVDEV
                     LLVDDSSTDATVELARELEINVVWHPHQVGYGGNQKTCYLQALQNGADVVVMLHPDGQ
                     YEPELIPSMIEPIVEGEADLVMGSRLLVPGWALDAGMPKWKFVANRFLTTIQNRIMGT
                     HLSEAHTGYRAY"
     gene            complement(514..1290)
                     /locus_tag="P5138_08770"
     CDS             complement(514..1290)
                     /locus_tag="P5138_08770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60710.1"
                     /translation="MSDRITGERASEKTGGFNPAWQRHSFAYAVAGERLGEGKVLDLG
                     CGTGHSFDRLAPRESVGVDISPDALVGQDRETLVADIREVPLPDDSFPSVLSSHSIEH
                     VPDPEKVVAEAARLLEEDGQAVFITPNRLTFGRPDEIIDPFHYIEFSPDEFEELCRKG
                     FSEVEVEGVYGSPRYMELFNEERKTLDRLLRLDPLRLRRLVPMRLKQWLYDFLLNHFR
                     KDDDPRAELIDQSDFEIRAGKVDDCLDVFAFCRGPLKETA"
     gene            1333..>1810
                     /locus_tag="P5138_08775"
     CDS             1333..>1810
                     /locus_tag="P5138_08775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRV60711.1"
                     /translation="MDASRLSKRWIWGLVGTLFLAGLVLRLLMLRESAWGDEMSTLWI
                     IRGNDFFGVISTVNSDAEISPPFFFLLAKVSSWIFGQTPTGIRIPSFVAGVLAVPMFY
                     LVGIRALGRRAALYATAIAAVSPFLVYFSANARAYSVMILLLLALVGLLLALLLTVL"
BASE COUNT          279 a          570 c          578 g          383 t
ORIGIN      
        1 agtaggcccg atagccggta tgagcttcgg agaggtgggt gcccatgatc cggttctgga
       61 tcgtcgtcag gaagcggttg gcgacgaact tccacttcgg catgccggcg tcaagcgccc
      121 agccggggac cagcagtcgc gagcccatca ccagatccgc ctcaccctcc acgatcggtt
      181 cgatcatgct gggaatcagt tccggctcgt actggccgtc gggatggagc atcacgacca
      241 cgtcggcgcc gttctggagg gcctggaggt aacaggtctt ctggtttccg ccgtatccga
      301 cctgatgcgg atgccagacc acgttgatct ccagttcgcg ggccagttcg accgtggcat
      361 cggtcgagga gtcatcgacc agcaacacct cgtcgaccgc gtccttgggg atctcggcaa
      421 aggttgcctc gagcgtctgg gcggcctgcc gcgccggcat cacgaccacg accttcggct
      481 tctttccggt gtccgtgtct tgcttctgcc cgctcatgcc gtctccttca gggggccgcg
      541 gcagaaggcg aagacgtcga ggcagtcgtc gactttgccg gctcgaatct cgaaatcgct
      601 ttggtcgatc agctcggccc gcgggtcatc gtccttgcgg aagtggttga gcaggaagtc
      661 gtagagccac tgcttcagcc tcatcggcac cagtcgcctg aggcgcagcg ggtcgagcct
      721 gagcagccgg tccagggtct ttctttcttc gttgaagagc tccatgtatc gcggggaacc
      781 gtaaacccct tcgacttcga cctcggagaa gcccttccgg cagagttcct cgaactcgtc
      841 cggactgaac tcgatgtagt ggaaggggtc gatgatttcg tcgggccggc cgaaggtgag
      901 ccggttgggg gtgatgaaga ccgcctggcc gtcttcttcg agcagccgcg ccgcctcggc
      961 cacgaccttc tccgggtccg gcacatgctc gatcgaatgg gaggaaagga ccgaggggaa
     1021 gctgtcgtcc ggcagcggaa cctcccggat gtcagcgacc agggtttcgc ggtcctggcc
     1081 gaccagggca tcgggggaaa tgtccacacc gaccgattcg cggggggcga ggcggtcgaa
     1141 gctatggccg gtgccgcagc cgagatcgag cacctttccc tcgcccagtc gctccccggc
     1201 gactgcatac gcgaaggagt gccgctgcca ggccggattg aacccgcccg tcttttcgct
     1261 cgcccgttcc ccggtgatcc tgtcactcat ccgggcgcat tattacgaca aggaaaatag
     1321 gctgccttgc ctgtggacgc atcccgtctg agcaaacgat ggatctgggg gctggtcgga
     1381 accctgtttc tggccggcct tgtgctgcgt ctgctgatgc tccgcgagtc ggcctggggc
     1441 gatgagatgt cgactctctg gatcatccgg ggcaacgact tcttcggggt gatctcgacc
     1501 gtgaactccg acgccgagat cagtccgccc ttcttcttcc tgctggcaaa ggtgagttcc
     1561 tggatcttcg gccagacccc caccggcatt cggatcccct cgttcgtcgc cggtgtcctc
     1621 gccgtgccga tgttctacct ggtcgggatt cgggccctgg gaagaagggc agcgctctac
     1681 gcgacggcga tcgcggcggt ctcgccgttc ctggtctatt tctcggccaa tgcccgcgcc
     1741 tattcggtga tgatcctgct gctgctggcc ttggttggtc tgctgctggc tttgctgctg
     1801 actgttctgc
//
LOCUS       DAOTIP010000471         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77066_length_1809_cov_2.469783, whole genome shotgun sequence.
ACCESSION   DAOTIP010000471 DAOTIP010000000
VERSION     DAOTIP010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77066_length_1809_cov_2.469783"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="P5138_08780"
     CDS             <1..1574
                     /locus_tag="P5138_08780"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRV60712.1"
                     /translation="RGFALIAWTFLSIGIILGARWSYTELGWGGYWAWDPVENASVMP
                     WLITTAYIHSIMVQEKRGMLKVWNVSLIVASFSLALLGTFLVRSGVLQSIHAFGDSTV
                     GPYFLSLIGVVLIGSTWLIISRLDILRSDRRIDSLASRESVFLINNLLLIALCAIVFW
                     GTFFPLISEFLTGTRSTLAAPWFDRYTTPVAVILVLFTGIGPMMSWRRISWEGAKRVF
                     TVPLIAAAIAFVLLLLFTDASNSVWALLIFTLAAFTVTALVQETWRAAAARKKNVGGS
                     IFASIWSNATRNRRRYGGYVVHLGLVVLLVGIAASSSFETKQDIELKPGQSAVVDDYK
                     VTYVHPTLARNDQALIFGADLQVDRNGESFAQLNPTRQYHQITGGGGSPGIASYFEGE
                     ATSEVGLKAGMRSDFWTSVEPNTKEFQRRARMADSLFGAFMRQKVLPLVQKNPSRAKV
                     VSAAATDLLSLTTDKVIAGYKKDNLAVPVTIRVIVSPMVSWIWVGAILAIIGALFAAW
                     PSGLLKRNRGRKKTA"
     gene            1571..>1809
                     /locus_tag="P5138_08785"
     CDS             1571..>1809
                     /locus_tag="P5138_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60713.1"
                     /translation="MTVVWFLILLVLVGAFVSMPFWRDRAASSTEDPEIAALEAARES
                     KYREIRDAEMDRESGKLSEEDFTELDAELRREAVG"
BASE COUNT          290 a          592 c          569 g          358 t
ORIGIN      
        1 cccgcggctt cgccctgatc gcctggactt tcctctcgat cggaatcatc ctcggtgcgc
       61 gctggtccta caccgagctc ggctggggcg gctactgggc ctgggacccg gtcgagaacg
      121 cctcggtcat gccctggctg atcaccacgg cgtacatcca ctcgatcatg gtccaggaaa
      181 agcggggcat gctgaaggtc tggaacgtga gcctgatcgt ggccagtttc agccttgccc
      241 tgctcggcac cttcctggtc cgatccgggg tgctccagtc gatccacgcc ttcggcgaca
      301 gcaccgtcgg tccgtatttc ctctctctga tcggggtggt cctgatcggc tcgacctggc
      361 tgatcatctc ccgtctcgac atcctcaggt cggaccgcag aatcgactcc cttgcctcgc
      421 gggaatcggt cttcttgatc aacaacctgt tgctgatagc cctttgcgcg atcgtcttct
      481 ggggaacatt cttcccgctc atctccgagt tcctgaccgg cacccgctcg accctcgccg
      541 cgccatggtt cgaccgctac accacacccg tggccgtgat cctggtcctc ttcaccggaa
      601 tcggcccgat gatgtcctgg cggcggatct cgtgggaggg tgcgaagcgg gtcttcacgg
      661 tgcccctgat tgccgccgcg atcgccttcg tgttgctgct gctcttcacc gatgcctcaa
      721 acagcgtctg ggccctgctg atcttcaccc tggccgcttt cacggtcacc gcgctggtcc
      781 aggagacctg gcgggctgcc gccgccagga agaagaacgt cggcggttcg atcttcgcct
      841 cgatctggtc caacgcaacc cgtaaccgcc ggcgctacgg cggctacgtc gttcacctgg
      901 gcctggtcgt gcttctggtc gggatcgccg cgtcgtcgag tttcgagacc aagcaggaca
      961 tcgagctgaa gccgggacag tccgcggtgg ttgatgacta caaggtcacc tacgtccacc
     1021 cgaccctggc ccgtaatgac caggcactga tcttcggcgc cgacctccag gtcgatcgca
     1081 acggcgagtc attcgcccag ctgaacccga cccggcagta ccaccagatc accggtggag
     1141 gcggctcccc gggcatcgcc tcctatttcg aaggggaggc gacgagcgag gttggcctga
     1201 aggccggaat gcggtcggac ttctggacct cggttgagcc gaacacgaag gagttccagc
     1261 gccgcgcccg catggctgac agcctcttcg gggctttcat gcggcagaag gtgcttccgc
     1321 tcgtgcagaa gaatccctcg cgggcgaagg ttgtgagtgc cgccgcgacg gacctgctca
     1381 gcctgaccac cgacaaggtg atcgccgggt acaagaagga caatcttgcg gtaccggtga
     1441 ccatccgcgt gatcgtcagc ccgatggtca gctggatctg ggttggcgcg atcctcgcga
     1501 tcatcggtgc gctgttcgcg gcctggccga gcgggctgct gaaacgaaac cgggggagga
     1561 agaagacggc atgaccgtgg tctggttcct gatcctgctc gtcctcgttg gcgcgttcgt
     1621 gtcaatgccg ttctggcgcg atcgtgccgc ttcgtcgacg gaggacccgg agatcgccgc
     1681 cctcgaagcg gcgcgggagt ccaagtaccg cgagatccgt gacgccgaga tggaccggga
     1741 gtcgggcaaa ctctcggagg aggacttcac tgaactggac gccgagctga ggcgcgaggc
     1801 ggttggcat
//
LOCUS       DAOTIP010000472         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77295_length_1805_cov_3.591429, whole genome shotgun sequence.
ACCESSION   DAOTIP010000472 DAOTIP010000000
VERSION     DAOTIP010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77295_length_1805_cov_3.591429"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="P5138_08790"
     CDS             <1..358
                     /locus_tag="P5138_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60714.1"
                     /translation="DQGAQEKAAANENESLPEGADDNKGEDGQEVADPGEAVSASAAA
                     KAEAAAVARTGGTVRQVTAETNDAPEQGDEQDNGEQASPAGTAYQVDLTKGSSELTVS
                     LDKQFNVLKVQTERAD"
     gene            complement(473..715)
                     /locus_tag="P5138_08795"
     CDS             complement(473..715)
                     /locus_tag="P5138_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60715.1"
                     /translation="MNEQEANARCAELAGSSPDRETHSWIAKEQADGTWMVVKLAVPP
                     PKAPESVKTVSEKDQAGKEDPRTPLEQNFPPYGIGM"
     gene            complement(755..1609)
                     /locus_tag="P5138_08800"
     CDS             complement(755..1609)
                     /locus_tag="P5138_08800"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HRV60716.1"
                     /translation="MVPIDIEPYKTDSSPEGYFAIAYVCVGDSKNPLPFKIDTGASNT
                     LFTPAAAKEAGLKTTEGDFSAGANCLIETRNAKLSGWSLAGMPLPETTVHVAKSPEVE
                     VGGITPVGLLGSDVLSRFGAFRVDYEGSRAVFTTIAPGEKIDPEALDDLAGGSPELKA
                     PMDVQSEQGSVTMTVPVRFGSGPQLKMSPDTGATWTIASSKVAKQQDLEPTGDTDKVG
                     TACGSTKVTFYESGDWTLAGSDLPAQEIGTTEGLVGIDGLLGADVMEHYGTVTFDYAG
                     GQLLLGTG"
BASE COUNT          346 a          566 c          558 g          335 t
ORIGIN      
        1 ggaccagggt gcccaggaga aggcagcggc caacgagaac gagtctctcc cggaaggtgc
       61 ggacgacaac aaaggcgaag acggccagga ggtggccgat ccgggcgagg cagtttcggc
      121 atcagccgca gcgaaggccg aggcagccgc ggtggccagg accggcggca cggtccggca
      181 agtgaccgcc gagaccaacg acgctcccga gcagggtgat gagcaggaca acggcgagca
      241 ggcctccccg gccggcaccg cctaccaggt cgacctgacc aagggttcca gcgaactgac
      301 cgtctctctc gacaagcagt tcaacgtact caaagtccag accgagcggg ctgactgacg
      361 cggtctggcg gtaggcggtg ggagcccgaa ccaactcagg ttcgggctcc cacaagcacg
      421 catgctgccc aggaaggctc gacgtcacga ccaagcagcc gggagaacca gttcacatcc
      481 cgatgccgta tggcgggaag ttctgttcga gcggggttct ggggtcctct ttgccggcct
      541 ggtccttctc gctgaccgtc ttcaccgatt cgggtgcctt cggtggcggg acagccagct
      601 tgaccaccat ccaggtgccg tcggcctgct ctttcgcgat ccagctgtgg gtttcccggt
      661 cggggctgct cccggccagt tccgcgcatc ttgcattcgc ctcttgctcg ttcatatgac
      721 ctatcttccc ccctctgcgc ggacccgggt gaaatcagcc cgtgccaaga agcaactggc
      781 cgccggcgta gtcgaaggtg accgtgccgt aatgctccat cacgtcggcc ccgagcaaac
      841 cgtcgattcc gacaagaccc tccgtcgtac cgatctcctg ggcgggaaga tccgaaccgg
      901 ccagggtcca gtctccgctc tcgtagaagg tgaccttggt gcttccgcag gcggtcccga
      961 ccttgtccgt atcacccgtc ggttcgaggt cctgctgctt cgccaccttt gaactggcga
     1021 tagtccacgt cgctcccgtg tccggtgaca tcttcagttg cgggcccgaa ccgaagcgga
     1081 ccggaaccgt catcgttacc gaaccctgct cggactggac gtccatcggg gccttcagct
     1141 caggtgatcc gccggcgaga tcgtccaatg cctcggggtc gatcttctct ccgggtgcaa
     1201 tggtcgtgaa gaccgccctc gagccttcgt agtcaacgcg gaaggcgccg aaccggctga
     1261 ggacatccga gccgagcagg cccacagggg tgatcccgcc gacctctacc tcaggcgact
     1321 tcgctacatg cactgtggtc tccggcaggg gcatcccggc cagcgaccag ccggagagct
     1381 tcgcgttcct ggtctcgatc aggcagttgg caccggcgga gaaatcgccc tccgtcgtct
     1441 tcagcccggc ttctttggcg gctgccggag tgaacagcgt gttcgaagcc ccggtgtcga
     1501 tcttgaaggg aaggggattc ttgctgtcgc cgacgcagac ataggcgatc gcgaagtaac
     1561 cctcgggact ggaatcggtc ttgtaaggct cgatgtcgat cggaaccacg gtcaggttcc
     1621 cgtccgtcga tgtctggctg cagccgtcgg ccgcggccag ggtcccatct ccggaatcag
     1681 aggaagagga gccgcgagaa aatgagacac aaattacggt gtgtggtttt tttgcgcata
     1741 aaagctggat tgatccttag aaattcaaaa attgatgttt caaaaattat ttaaaaataa
     1801 ctaga
//
LOCUS       DAOTIP010000473         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77368_length_1804_cov_3.213836, whole genome shotgun sequence.
ACCESSION   DAOTIP010000473 DAOTIP010000000
VERSION     DAOTIP010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77368_length_1804_cov_3.213836"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /gene="trxA"
                     /locus_tag="P5138_08805"
     CDS             complement(<1..577)
                     /gene="trxA"
                     /locus_tag="P5138_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRV60717.1"
                     /translation="MVKDVDEASFQQEVIERSKEVPVVVDFWAEWCGPCRTLGPAIEQ
                     AVSERAGKVELAKVDVDSNQQLAAAFRVQGIPAVKAFVNGEMVDEFTGALPPAQISAF
                     LDRIAPAEQKSEEELAEERAKELESNEFYIAGREAREALEASENPPTEAFKAWDDGDH
                     EFAFDLLQKEVEVADPDRRDLLRKVMVGYFTE"
     gene            881..>1804
                     /locus_tag="P5138_08810"
     CDS             881..>1804
                     /locus_tag="P5138_08810"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HRV60718.1"
                     /translation="MSPSDPPFDSIEEWRKLPYRERLKPLAQDWAVNGIGVPQAVYLL
                     YIVKLVVYAGGGLLLISATSDLGGLSKIGDWWTQPVVFQKAVVWTMLWEALGLGAGSL
                     PLTLRFSPMVGGFLHWLRPGTIRQPPWPDKVPLTAGDSRAIFDVFLYAGFIASAIVLL
                     VGSGADGFDPVIVGILLAFLVLLGFRDKVPFLAARAEVYGPFLLIFCFPADQMIVAAQ
                     FVMFCIWWGAASSKLNKHFPFVVTVMISNTPWNRSKWAKKKLYRNHPEDLIPSRIGEF
                     NAHMGTVLEFTLPLLLIASSGGVIGTIAVAGM"
BASE COUNT          253 a          566 c          616 g          369 t
ORIGIN      
        1 gctcggtgaa gtagccgacc atcaccttgc ggagcaggtc tcggcggtcc gggtcggcga
       61 cttcgacttc cttctggagc aggtcgaagg cgaactcgtg gtcgccgtcg tcccaggcct
      121 tgaaggcctc ggtcggcggg ttctccgatg cctcgagcgc ctcgcgggct tcgcggccgg
      181 cgatgtagaa ctcgtttgat tcgagttcct tcgcccgttc ttcggccagc tcttcttcgg
      241 acttctgctc ggccggagcg atgcggtcga ggaaggcgct gatctgggcc ggggggaggg
      301 cgccggtgaa ctcgtcgacc atctcgccgt tcacgaaggc cttgacggcc gggatgccct
      361 ggacgcggaa ggcggcggcg agctgctggt tggaatcaac gtcgaccttg gccagttcga
      421 ccttgccggc gcgttcgctt accgcctgct cgatcgccgg gccgagggtg cgacaggggc
      481 cacaccactc ggcccagaaa tcgacgacga ccgggacctc tttggagcgc tcgatgactt
      541 cctgctggaa gctcgcctca tctacgtctt tgaccatgga ccgagcatag tgggaccggg
      601 tcaccggctc gcctggtcgc aggcggagtt gaggccggag cggctagcgg cgacggttgg
      661 ccggatgctc cgggttgagg ctccgggtct gcttcgaggc gttgacggtg agtgacatga
      721 gaagggaacg catgggaatc tccttggact gggggacttg acgctctgga gactcccgga
      781 cgggccttgt cgggttgttg cggggatatt gcggtggctg aatcgggcga taagctccgg
      841 tcgtccgagg tcttctgagg aggggtgctt catgggtttc atgagtccga gtgatccgcc
      901 gtttgactcg atcgaagagt ggcggaagct tccctatcgg gaacggctga agcctctggc
      961 ccaggactgg gcggtcaacg ggatcggtgt gccgcaggcg gtctacctgc tctacatcgt
     1021 gaagctcgtg gtctatgcgg gtggcgggct gctgctgatt tccgccacct ccgacctcgg
     1081 cgggctctcg aagatcggcg actggtggac ccagccggtc gtcttccaga aggcggtcgt
     1141 ctggaccatg ctctgggagg cgctcggcct cggggccggc tcgctgcctt tgaccctgcg
     1201 tttctcgccg atggtcggcg gcttcctcca ttggctgagg cccgggacga tccgccagcc
     1261 gccgtggccc gacaaggtgc cgctcaccgc gggcgacagc cgcgccatct tcgacgtgtt
     1321 cctctatgcc ggcttcatcg ccagcgcgat cgtgttgctg gtcggcagcg gcgccgacgg
     1381 cttcgatccg gtgatcgtcg ggatcctgct cgcctttctg gtcctgctcg gcttccgcga
     1441 caaggtgccg ttcctcgccg cccgggccga ggtctacggg ccgttcctcc tcatcttctg
     1501 cttcccggcc gaccagatga tcgtcgccgc gcagttcgtc atgttctgca tctggtgggg
     1561 cgccgcctcc tcgaagctga acaagcactt ccccttcgtg gtcacggtaa tgatctccaa
     1621 caccccgtgg aaccggagca aatgggcgaa gaagaagctc taccgcaacc acccggagga
     1681 cctgatcccc tcccggatcg gcgagttcaa tgcccacatg ggcacggtgc tcgagttcac
     1741 cctgccgctg ctgctgatcg cctcatccgg cggggtgatc gggacgatcg cggtcgccgg
     1801 gatg
//
LOCUS       DAOTIP010000474         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77525_length_1802_cov_2.035489, whole genome shotgun sequence.
ACCESSION   DAOTIP010000474 DAOTIP010000000
VERSION     DAOTIP010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77525_length_1802_cov_2.035489"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1802
                     /locus_tag="P5138_08815"
     CDS             <1..>1802
                     /locus_tag="P5138_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV60719.1"
                     /translation="NEVTEIAAAAHVHTIKKYGPDRIAGFSPIPAMSQVSYAAGSRFL
                     SLIGGVPLSFYDWYADLPPASPQVWGDQTDVPESADWWDAAYLIMWGSNIPQTRTPDA
                     HFMTEARYRGQKTIVVSPDYAGNTKFADQWLPATAGTDGALAMAMGHVILKEFFVDRQ
                     AEYFDGYARQFTDMPFLVELEDHGDGTVHGGPMLRASDLGETGENSEWKTVLVDSRTD
                     QAVVPNGTIGERWGEEGEGKWNLDLEGIEPALTLLGRHDELVEVTLPRFDGGETEGGT
                     TMVRGVPARRIGGKLVTTVFDLTLAQYGVAREGLPGTWPKDYSDPAEPYTPAWQEEHT
                     GVSAAVCEQVAREFAENAERTRGRSMIVMGAGINHWYHADQTYRAILSMLLFCGCQGV
                     NGGGWAHYVGQEKVRPITGWQTLAFALDWNRPPRHQASTPFWYLATDQWRYETRRPDE
                     FVSPAGDGKFEHTHIVDANAKAVRLGWTPSAPALSRNPLELADEAKAAGKSSAEYVVE
                     ELKEGRLGFACEDPDDPANFPRVLNLWRANLLGSSSKGHEYFLKHLLGTDHAVQTPEC
                     EPDRRPEDVKWRDEAPEGKLDLFMTIDFRMNGSC"
BASE COUNT          318 a          640 c          579 g          265 t
ORIGIN      
        1 ggaacgaggt cacggagatc gccgccgcgg cccacgttca cacgatcaag aagtacgggc
       61 ccgaccgcat tgccggcttc agcccgatcc cggccatgtc ccaggtctct tacgccgccg
      121 gcagccgttt cctttccctg atcggcgggg tgccgctcag cttctacgac tggtacgccg
      181 acctgccccc ggcctcgccg caggtctggg gagatcagac tgacgtaccc gagtcagcgg
      241 actggtggga tgccgcctac ctgatcatgt ggggttcgaa catcccgcag acccgcacgc
      301 ccgacgccca cttcatgacc gaggcccgct accgcggcca gaagacgatc gtggtctcgc
      361 ccgactacgc cggcaacacc aagttcgccg accagtggct gcccgccacc gccggcaccg
      421 atggcgccct ggcgatggcg atgggccatg tgatcctcaa ggagttcttc gtcgaccggc
      481 aggccgagta cttcgacggc tacgcacgcc agttcacgga catgcccttc ctggtcgaac
      541 tcgaagacca cggtgacggc accgtccacg gcggtcccat gctgagggcc tcggacctcg
      601 gcgagaccgg tgagaactcc gagtggaaga ccgtcctggt cgactcccgg accgatcagg
      661 cagtggtccc gaacgggacg atcggcgagc gctggggcga ggagggcgag ggcaagtgga
      721 acctcgacct cgaaggcatc gagccggccc tgaccctgct cggccgccac gatgaactgg
      781 tcgaagtgac cctgccgcgc ttcgacggcg gcgagaccga aggcggcacc acgatggtcc
      841 gcggcgtgcc ggcccggcgg atcggcggca agctggtcac cacggtcttc gacctgaccc
      901 tcgcgcagta cggggtcgcc cgcgaaggcc tgcccggcac ctggcccaag gattactcgg
      961 acccggccga gccctacacc cctgcatggc aggaggaaca caccggcgtt tcggccgcgg
     1021 tctgcgaaca ggtcgcccgc gagttcgctg aaaacgcgga gcggacccgg ggccggtcga
     1081 tgatcgtgat gggggccggg attaaccact ggtaccacgc cgaccagacc taccgggcga
     1141 tcctctcgat gctgctcttc tgcggctgcc agggcgtcaa cggtggcggc tgggcccatt
     1201 acgtcgggca ggagaaggtc cgaccgataa ccggctggca aacgctcgcc ttcgcgctcg
     1261 actggaaccg gcctccgcgc catcaggcgt cgaccccgtt ctggtacctg gccaccgacc
     1321 agtggcgcta cgagacccgc cgcccggacg agttcgtttc accggccggt gacggcaagt
     1381 tcgagcacac ccacatcgtc gacgccaacg ccaaggccgt gcgcctcggc tggacccctt
     1441 cggccccggc gctcagccgc aaccctcttg aactggccga cgaggcaaag gcggccggca
     1501 agtcctcggc cgaatacgtg gtcgaggaac tgaaggaagg ccggctcggc tttgcctgcg
     1561 aggatccgga cgatcccgcc aacttcccgc gggtcctcaa cctctggcgg gccaacctgc
     1621 tcggttcatc gagcaagggt cacgagtact tcctcaagca cctgctcggt accgatcacg
     1681 cggtgcagac cccggaatgt gaaccggacc gccgccccga ggacgtgaag tggcgggacg
     1741 aagcccccga aggcaagctc gacctgttca tgacgatcga cttccgcatg aacggctcct
     1801 gc
//
LOCUS       DAOTIP010000475         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77554_length_1801_cov_3.103093, whole genome shotgun sequence.
ACCESSION   DAOTIP010000475 DAOTIP010000000
VERSION     DAOTIP010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77554_length_1801_cov_3.103093"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /gene="map"
                     /locus_tag="P5138_08820"
     CDS             <1..656
                     /gene="map"
                     /locus_tag="P5138_08820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRV60720.1"
                     /translation="VSDPLLSQPGEKFIRSQGGVPTFKGFRGFPGSICASPNSMVVHG
                     IPGPYTLEKGDILSVDIGVTLDGWVADGASTIPVGPIDETTQKLLTVTKESLDLAAAQ
                     MVPGNRVGDIGHAVQSHVEAAGFSIIRTLVGHGVGQNMHEEPQIPNYGTPGRGPEIEE
                     GMVFAIEPMVNVGGPHVYMDDDGWSVFSEDGSMAAHFEYTVAATADGPRILTPWDRE"
     gene            763..876
                     /gene="rpmJ"
                     /locus_tag="P5138_08825"
     CDS             763..876
                     /gene="rpmJ"
                     /locus_tag="P5138_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRV60721.1"
                     /translation="MKVRASVKPMCEKCKIIRRRGRVLVICENPRHKQRQG"
     gene            880..1257
                     /gene="rpsM"
                     /locus_tag="P5138_08830"
     CDS             880..1257
                     /gene="rpsM"
                     /locus_tag="P5138_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRV60722.1"
                     /translation="MARIAGVNIPLNKRAVIGLTYVYGIGQSTAADILEEVGIDPDTQ
                     VKDLTEDEVIKLRDAVETREVEGDLRRERSQNVKRLMEIGSYRGLRHRRGLPVRGQRT
                     KTNARGRKGPRRMSVAGKKKPAK"
     gene            1275..1667
                     /gene="rpsK"
                     /locus_tag="P5138_08835"
     CDS             1275..1667
                     /gene="rpsK"
                     /locus_tag="P5138_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRV60723.1"
                     /translation="MAAKKPAKSRRRVRKNVAVGQAHIKTSFNNTIVTLTDPEGNVIA
                     WESAGSAGFKGSRKSTPFAAQVTADAAAKKGMEHGLQKVEVFAKGAGSGKDTAVRSLQ
                     AAGLEVTSVKDVTPQAHNGCRPRKRRRV"
BASE COUNT          374 a          590 c          544 g          293 t
ORIGIN      
        1 aagtgagtga ccccctcttg agccagcctg gggagaagtt catccgctcc cagggcggag
       61 tgccgacctt caagggcttt cgcggatttc ccggttcgat ctgcgcctcc ccgaactcga
      121 tggtcgttca cggcatcccc ggtccctaca ccctcgagaa gggtgacatc ctctcggttg
      181 acattggggt cactctcgat gggtgggttg ccgacggtgc ctcgaccatt ccggtcggcc
      241 cgatcgacga gaccacgcag aagctgctca ctgtgaccaa ggagtcactg gacctcgcgg
      301 ccgcgcaaat ggttccgggc aaccgggtgg gagacatcgg ccacgcggtc cagagccatg
      361 tcgaagcagc cggattttcg ataatccgca ccctggtcgg ccacggagtc ggccagaaca
      421 tgcatgagga accgcagatc ccgaactatg gaacccccgg ccgcggaccg gagatcgagg
      481 aaggcatggt cttcgcgatc gaaccgatgg tcaacgtcgg cggcccccat gtctacatgg
      541 atgacgacgg ctggtcggtc ttctccgaag acggctccat ggcggcccat ttcgagtaca
      601 cggtcgccgc cacagccgac ggcccccgca tcctcactcc ctgggacagg gaatgatcca
      661 agctgctatt gttcccaagc ggccaaaatt ggccgttttt ccacgccctg agtccgttta
      721 ctgggactca cgggacatct ctttcaaagg aacgttagga cgatgaaggt ccgagcctcg
      781 gtaaaaccga tgtgtgagaa gtgcaagatt attcgccggc gcggacgcgt cctggtgatc
      841 tgtgagaacc cgcgacacaa gcagaggcag ggttagtaga tggcgcgtat cgccggggtc
      901 aacatccccc tgaacaagag ggccgtgatc ggcctcacct acgtctacgg gatcggtcag
      961 tcgaccgccg cggacatcct cgaggaagtc ggcatcgatc ctgacaccca ggtcaaggac
     1021 ctgaccgaag acgaggtcat caagctgcgc gacgcggtcg agacccgcga ggtcgaaggt
     1081 gacctgcgtc gcgagcgttc ccagaacgtc aagcgcctga tggaaatcgg ctcctaccgg
     1141 ggcctgcgcc accgccgcgg actcccggtc cggggtcagc gcaccaaaac caacgcccgg
     1201 ggccgcaagg gcccgcgccg catgagcgtt gcgggcaaga agaagcccgc caagtaagga
     1261 gctggagaac tttcatggca gccaagaagc cggccaagtc ccgtcgtcgc gtccgcaaga
     1321 acgtcgccgt cggacaggcg cacatcaaga cttccttcaa caacaccatc gtcaccctga
     1381 ccgatcccga gggcaacgtc atcgcctggg aatcggccgg tagcgccggt ttcaagggtt
     1441 cgcgcaagtc gaccccgttt gccgcccagg tgaccgccga cgcggccgcc aagaagggca
     1501 tggaacacgg cctccagaag gtcgaagtct tcgccaaggg cgccggttcc ggcaaggaca
     1561 cagcggttcg ttcactgcag gccgccgggc tcgaggtgac cagcgtcaag gacgtcacgc
     1621 cgcaggccca caacggctgc cgtccccgca agcgccgtcg cgtctgattt ccggccgcat
     1681 cgccagcctt cccctccccc gcaacaaaag gatccaatga gcacagattt ccaggtaccg
     1741 accatctcgt ccgagcaggt tgaagagaac aagggtgttt tcaccatcga gcccctcgac
     1801 a
//
LOCUS       DAOTIP010000476         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77606_length_1800_cov_3.356447, whole genome shotgun sequence.
ACCESSION   DAOTIP010000476 DAOTIP010000000
VERSION     DAOTIP010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77606_length_1800_cov_3.356447"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..>1800)
                     /locus_tag="P5138_08840"
     CDS             complement(100..>1800)
                     /locus_tag="P5138_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV60724.1"
                     /translation="GSMVFAWMAMGMTVLIPWLVGQAVNAVQDSDQTQLFWMVGAILV
                     AAVLRLVLTVIRRIVAGRVSLAVEFDLRQEVYAHLMSLELGFFDRNQTGQLMSRATVD
                     LQSIRFFLGYGLIFFSQSILTVLLAGVVMFVINPWLALIALAPAPLIVITALRYTRQS
                     RPAMQEVAQRVAELTAQAEESVAGIRVVKAFAREDHQFERFQGAVDRLFDQSIRSTRI
                     QAFFTPLIGALPQIGTALVLLVGGHEVLNGSLDLGQFTAFYTYMLMLAGPLRTVGMAL
                     GMGQRAIAAGNRLFEVLDREPRLTSRPDAIPLPEGGGAVSFDSVSLRYGPEAPEALLD
                     IDLDVPAGSRLALVGPSGSGKTSLAALIARLYDPTEGEVSIDGTDLRDLEIPSLRHGI
                     AYVSDDSFLFSDTIANNISYARADATPGEIERAARLAQASEFIEALPDGYETVVGERG
                     LTLSGGQRQRVAIARALLANPRILILDDATSSLDATTEQQIRAGLAEAMAGRTTFVIG
                     HRLSTISLADECVLMDEGRIIDRGTIAELTDRSSLFRELTGGSGDEPLFVPEDEEVLA
                     "
BASE COUNT          301 a          587 c          630 g          282 t
ORIGIN      
        1 tgcgaccccg tccggcgacg atctcccggt gtccgttcgc ttcgcgcggc tcggtggctt
       61 tgcccgaccc ggaggccgtc ggttctctgc gctctgcgct cacgccagca cctcctcgtc
      121 ctcgggcacg aagagcggct cgtcgcccga gccaccagta agttcacgga agagggagga
      181 acgatcggtc aattcggcaa tcgtgccccg gtcgatgatc cggccctcgt ccatcagcac
      241 gcattcgtcg gccaggctga tcgtggacag ccggtggccg atcacgaagg tcgtgcggcc
      301 tgccatcgct tcggcgagtc ctgcccggat ctgctgttcg gtcgtggcgt cgagggatga
      361 agtcgcgtcg tcgaggatca ggatccgcgg gttcgcgagc agggcccggg cgatcgcgac
      421 ccgctggcgc tggcctccgg agagggtgag gccccgctcg ccaaccacgg tctcgtaccc
      481 gtccggcagg gcctcgatga attcggaggc ctgggccagc ctggctgccc gttcgatttc
      541 cccgggggtc gcgtcggcgc gggcgtagga gatgttgttg gcgatcgtgt cgctgaacag
      601 gaagctgtca tcggatacat aggcgatccc atgcctcaat gacggaatct cgagatcacg
      661 caggtcggtc ccgtcgatcg agacttctcc ctcggtcggg tcgtagaggc gggcaatcag
      721 ggccgccagg ctcgtcttgc cggaaccgga ggggccgacc agggccagcc gcgagccggc
      781 cggcacgtcg aggtcgatgt cgagaagcgc ctcgggggcc tcagggccgt agcgaagcga
      841 caccgagtcg aagctgaccg cgccgcctcc ctccggcagc ggaatcgcgt ccggccggga
      901 ggtgagtcgc ggctcgcggt cgagtacctc gaagagccgg ttgcccgcgg cgatcgcccg
      961 ctgtcccatc cccagtgcca tgccgaccgt gcgcaggggc ccggccagca tcagcatgta
     1021 ggtgtagaag gcggtgaact gccccaggtc aaggcttccg ttcagcacct cgtgaccgcc
     1081 gaccagcagg accagggcgg tgccgatctg tggcagggct ccgatcaggg gagtgaagaa
     1141 ggcctggatc cgggttgaac ggatgctctg gtcaaagagc cggtcgacgg cgccctggaa
     1201 ccgctcgaac tgatggtcct cccgggcgaa ggccttgacc acgcgtatcc cggccacgct
     1261 ctcctcggcc tgggcggtca gttcggcgac gcgttgcgcc acttcctgca tcgccggccg
     1321 ggactgtcgg gtgtacctga gggcggtgat cacgatcagc ggagcggggg cgagggcgat
     1381 cagcgccagc caggggttga tcacgaacat gacgaccccg gcgagcagca cggtgaggat
     1441 gctctggctg aagaagatca gcccgtagcc gaggaagaag cggatcgact ggaggtccac
     1501 ggtcgcgcgg ctcatcagct ggccggtctg gttgcggtcg aagaagccga gctcgaggct
     1561 catcaggtgg gcatagacct cctgccgcaa gtcgaactcc acggccagcg agacccgccc
     1621 ggcgactatg cggcggatca ccgtaaggac gaggcggaga accgccgcca cgaggatcgc
     1681 cccgaccatc cagaagagct gggtctggtc gctgtcctgg accgcattca cggcctggcc
     1741 cacgagccag ggaatgagca cggtcatgcc catcgccatc caggcgaaga ccatcgaacc
//
LOCUS       DAOTIP010000477         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_77795_length_1797_cov_2.771527, whole genome shotgun sequence.
ACCESSION   DAOTIP010000477 DAOTIP010000000
VERSION     DAOTIP010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77795_length_1797_cov_2.771527"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="P5138_08845"
     CDS             complement(<1..1024)
                     /locus_tag="P5138_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60725.1"
                     /translation="MKKPAVLLAVFASVFALAACGSNRNPEVTPDTLASAMDQVCDDT
                     TADFEAMGTRGLTMPAIAAEFQGYAEVRQAVIDGFNELNTDDAAKKEMEPYIEASEEV
                     VAQDKAIARAAGNDDQKAMNQAFLAQNAAFTEREKAAGEIGTEVCGQQAEIKVEPTGT
                     EPPEDLSAYQPKNTVEDAADKYLKSARSGDCGQINANRHADAGDLDAPNCASVSKALA
                     GAKVAGTESYGPVGQAEIVGSDGTHFPTYYVTDLDGNLRYGGDAIHDAGGLRPAPEGN
                     DAQATAEATVNAIREDDAAAFNQTLPFEDSPFWIKGEKVNSFGDGEYNAEFIGDIKEG
                     DTEPVQL"
     gene            complement(1114..1695)
                     /gene="scpB"
                     /locus_tag="P5138_08850"
     CDS             complement(1114..1695)
                     /gene="scpB"
                     /locus_tag="P5138_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRV60726.1"
                     /translation="MSELSPLARRVESLLFLSNQPVTEISLAEACEVELDEVQEAVGE
                     LEADLAPGRRGLLLRRVGGGLILASDPESETAARRLLSKPRTPPLTQAQAETLAIVAY
                     LQPVARPEIARIRGVSSESAVNSLSERGLIEESGRSRFGAAIYRTSPLFEKVFGLEGL
                     EALPDPTRFDPTPDDEREIRERLLAAGEQRAES"
BASE COUNT          247 a          594 c          587 g          369 t
ORIGIN      
        1 cgagctgaac cggctccgtg tcaccctcct tgatgtcccc gatgaattcc gcgttgtatt
       61 ccccatcgcc gaatgagttg accttctctc ccttgatcca gaacggactg tcctcgaagg
      121 gcagcgtctg gttgaaggcg gcggcgtcgt cttcgcggat cgcgttgacg gtcgcctccg
      181 cggtcgcttg cgcatcattt ccctcggggg ccgggcgaag gccgcccgcg tcgtggatcg
      241 cgtcgccgcc gtatcggagg ttgccgtcca gatcggtgac gtagtaggtc gggaagtggg
      301 ttccgtcgga gcccacgatt tcggcctggc cgaccgggcc gtagctctcg gtgcccgcca
      361 ccttcgcccc ggccagggcc ttgctgaccg aagcgcagtt cggcgcgtcg aggtcgccgg
      421 cgtcggcgtg acggttggcg ttgatctggc cgcagtcgcc ggaccgggcc gacttgaggt
      481 acttgtccgc ggcatcttca acggtgttct tcggctggta tgcgctcaga tcttccggcg
      541 gctcggtgcc ggtcggttcg accttgattt ccgcctgctg cccgcagacc tcagtgccga
      601 tttcgccggc tgccttttcc cgttcggtga aggcggcgtt ttgggccagg aaggcctggt
      661 tcatcgcctt ctggtcgtcg ttgccggctg ccctggcgat cgccttgtcc tgggccacga
      721 cctcctccga agcctcgatg tagggctcca tctccttctt cgcagcgtcg tccgtgttca
      781 gttcattgaa gccgtcgatt accgcctggc gcacctcggc gtaaccctgg aactcggcgg
      841 cgatcgccgg catggtcagg ccgcgggtcc ccatcgcttc gaagtccgcc gtggtgtcat
      901 cacatacctg atccatcgcc gatgccagcg tgtccggcgt cacctccgga ttgcggttgc
      961 tgccgcaggc ggcgagggca aaaacggatg cgaacacggc aagaagtacg gcaggcttct
     1021 tcaaaggaat ctccctcgga ttgttgggcc gggcaactcg gcgtccggaa acctaccgcc
     1081 tggcccggaa gcgagaggcc gtgacccggt ctctcaggac tcggcccgct gctcgccggc
     1141 ggcgaggagg cgttcccgga tctcgcgttc gtcgtccggt gtgggatcga accgggtcgg
     1201 atcgggcagg gcctctagcc cctcgagccc gaaaaccttc tcgaatagcg gtgaggtcct
     1261 gtagatcgcc gctccgaagc gtgaccttcc cgactcctcg atcaggccgc gctcgctgag
     1321 actgttgacc gctgattcgg acgagacacc ccggatgcgg gcgatttcag gccgggctac
     1381 gggctgaagg taggcgacga tcgccagcgt ttcagcctgg gcctgggtga gcggcggggt
     1441 tcgcggcttt gagagcagac gtcgcgccgc cgtttcggat tccgggtcgc tggcgaggat
     1501 aagccctcct ccgacccgtc tcagcagcag gccgcggcgg ccgggagcga gatccgcttc
     1561 cagctcgccc actgcttcct ggacctcgtc cagttcgacc tcgcaggctt cagccaggga
     1621 gatctcggtc accggctggt tcgagaggaa gagcagcgat tcgacccttc tggcgagggg
     1681 gctcagttca ctcatgccgt tttcgccaat ccgttctggc cggcctgtac gcgccggacc
     1741 gtgatcgggc cgcaggattc ggtctggtcc caaaccgcct cccccttctt gtaaagg
//
LOCUS       DAOTIP010000478         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_78026_length_1793_cov_4.254315, whole genome shotgun sequence.
ACCESSION   DAOTIP010000478 DAOTIP010000000
VERSION     DAOTIP010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78026_length_1793_cov_4.254315"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="P5138_08855"
     CDS             <1..628
                     /locus_tag="P5138_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRV60727.1"
                     /translation="THDDDRILIVDDNSPDGTGTIADEIAAGNPRVRVIHREKKEGLG
                     PAYLEGFRTALDGGADLIVQMDADFSHDPAYLPRLLAASELADLVIGSRYVPGGGITE
                     WGQVRRILSRGGSFYSRTILRVPVRDMTGGFKCFRREVLESIDLSEVAASGFSFQVEM
                     TWRVIKAGFSVMEVPITFRERQEGDSKMTTAIVAEAAWRVPAMRIRNR"
     gene            802..1125
                     /gene="trxA"
                     /locus_tag="P5138_08860"
     CDS             802..1125
                     /gene="trxA"
                     /locus_tag="P5138_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRV60728.1"
                     /translation="MALPEVTDATFKAEALDADGPVLVDFWAPWCGPCRVVHPVLEEI
                     NQERDDLKIVSLNIDDNPQTAQQYGVLSIPTMILLKDGAMVKQLVGAQPKRKILAELE
                     PALQG"
     gene            1145..1324
                     /locus_tag="P5138_08865"
     CDS             1145..1324
                     /locus_tag="P5138_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HRV60729.1"
                     /translation="MPFVQIHWFEGRSDEQKAEIAKRIEDALIEVAGAAPEHCWVKFE
                     DSKKTDFIIPEARPE"
     gene            1353..1775
                     /locus_tag="P5138_08870"
     CDS             1353..1775
                     /locus_tag="P5138_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV60730.1"
                     /translation="MGYRRLAAEDQFWRRSNMMGVLNTDLARQLEAEKLGARLWRIEP
                     GQASTRHRHKTTEELYVLLEGTGKLRVEDELLVLEPMDAVVVDPETVRQPFNDTGKDQ
                     LWLVVGAPTEHADTTTMDEELIAWMYPDGPKALPPELN"
BASE COUNT          350 a          547 c          592 g          304 t
ORIGIN      
        1 cactcacgat gacgaccgga tcctgatcgt cgacgacaac tcgccggacg ggaccgggac
       61 gattgccgac gagatcgccg ccggcaatcc cagggtgcgg gtcatccacc gcgagaagaa
      121 ggaaggcctc gggccggcct acctggaggg cttccgtacc gcgctggatg gcggggcgga
      181 tctgatcgtc cagatggacg cggacttctc ccacgatccg gcctatcttc cgaggctgct
      241 ggctgcgtcg gagctggccg acctggtgat cggttcgcgc tacgtgccgg gcggcggaat
      301 caccgagtgg ggtcaggtcc ggcgaatcct gagccgcggc ggaagctttt actcgcggac
      361 gatcctgcgc gttcccgtcc gggacatgac cggaggcttc aagtgcttcc ggcgcgaggt
      421 gctcgagtcg atcgacctgt ccgaggtcgc cgcgtcgggc ttttccttcc aggtggaaat
      481 gacctggcgg gtgatcaagg ccggtttctc ggtgatggag gttccaatca ccttccggga
      541 aaggcaggag ggggattcga agatgaccac cgcgatcgtt gcggaggccg cctggcgggt
      601 cccggcaatg cgcatccgga atcgttgaca accagacccg gcccatccat cctttgtttc
      661 cgtgggccgg cgggttcaat atgcagggcg ggtcggatcc ggcccgactt gagaaggttc
      721 acttcatttt gtatagtgac ctgtgagaca ggaatcgaaa cgcgggaatg gacccgctga
      781 aagacgatca ggagaccgaa aatggcactt cccgaagtaa ccgacgcgac cttcaaggct
      841 gaagctctcg atgccgatgg cccggtgctg gtcgacttct gggctccttg gtgcggtccc
      901 tgccgcgtgg tccatccggt cctcgaggag atcaaccagg agcgcgacga cctcaagatc
      961 gtcagcctca acatcgatga caacccgcag actgcccagc agtacggagt tctctcgatc
     1021 ccgacgatga tccttcttaa ggacggcgcc atggtcaagc agcttgtcgg cgcccagccc
     1081 aagcgcaaga tcctcgccga gctcgagccg gccctgcagg gctgaccgga acggaccggc
     1141 tgacatgccg ttcgtccaga tccactggtt cgagggccgc tccgacgagc agaaggccga
     1201 gatcgccaag cgcatcgagg acgcgctgat cgaagtggcc ggggcggctc ccgaacactg
     1261 ctgggtcaag ttcgaagact cgaagaagac agacttcatc attcccgaag cccggcccga
     1321 gtagccgggc ttcgtcctta aggaggggtt tcatgggata ccgccgactc gccgcggagg
     1381 atcagttctg gcgccgctcc aacatgatgg gcgtgctcaa caccgacctg gcccgccagc
     1441 tcgaagcgga gaagctcgga gcgaggttgt ggcggatcga gcccgggcag gcctcgaccc
     1501 ggcatcgcca caagaccacc gaggagctct acgtgctgct cgagggaacc gggaaactga
     1561 gggtcgaaga cgagcttctg gtgcttgagc cgatggacgc cgtggtggtt gatccggaga
     1621 cggtgcgaca gccattcaac gacaccggga aagaccagct ctggctcgtg gtgggcgccc
     1681 cgaccgagca tgccgacacc accacgatgg acgaggaact gatcgcctgg atgtatccgg
     1741 atggtccgaa agccctgcca cccgagctga actgagaagc ttccggcatg gag
//
LOCUS       DAOTIP010000479         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_78162_length_1791_cov_3.074885, whole genome shotgun sequence.
ACCESSION   DAOTIP010000479 DAOTIP010000000
VERSION     DAOTIP010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78162_length_1791_cov_3.074885"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="P5138_08875"
     CDS             <1..1422
                     /locus_tag="P5138_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRV60731.1"
                     /translation="VARVLAEPRARPRRFDPGLETSVENPDGTITETLAADSYIPASG
                     LAILTCSGGDSGIAADQAELTGLRFAELLPETRQRLKELLPEAATVANPLDYTSLLWF
                     ETDRLTEIVATVGACETVEQMLIFHDHPQDLRPEHESEWSVVREALAEAARRSGCPAM
                     LASTLPDLVSEEGRRNLAGTGIPVVGGMRAAIAAAAANQKSRRRPDELVTERLMAIAA
                     ATREASGPARVDRANVGEGNDTVGNAADGRWLAEHEVKAVLREAGIAVPAFLTAGSPG
                     ACLDAAAGLSFPLVLKLSGPSIQHKSELGAVRVGIEDEAMLLRHAEDLMSIAGQIHLE
                     QGLAGEESTPPPVFLVEEMAGAGVEMIVTARSDAVVPSLTIGIGGTWAEDVNDSVVMP
                     LPVSPEDALRALGRLKAFGLLRPRGGGGADTIALAELASGLGRLLLDYRDGEGRGLDL
                     IELNPVVAGQDGATALDALAHLA"
     gene            complement(1437..>1791)
                     /locus_tag="P5138_08880"
     CDS             complement(1437..>1791)
                     /locus_tag="P5138_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60732.1"
                     /translation="ALKSLLDIPIVGEVRGTGHFWAIEVVRDQETKEPFQGEESETLF
                     KDVLSEALWERGLLCRLDDRSDPIIQIAPPLIAEPELFHEIASILREGLELTASRIHG
                     AAPPLLPPQSKTRPI"
BASE COUNT          277 a          618 c          625 g          271 t
ORIGIN      
        1 gtcgcgcgcg tgctcgccga gccgcgtgcc cgtccgcgaa ggttcgaccc cgggctggag
       61 acatcggtcg aaaacccgga cggcacgatc accgagaccc tcgccgccga ctcgtacatc
      121 ccggcctccg gcctcgccat cctcacctgc tcgggtggcg actccggaat cgcggctgac
      181 caggccgaac tgaccggtct gaggttcgcc gagctcctgc cggaaacccg ccaaagactc
      241 aaggaactgc ttcccgaggc ggcgaccgtc gccaatcccc tcgattacac ctctctgctc
      301 tggttcgaaa ccgaccggct gaccgagatc gtcgcgaccg tcggagcctg cgagacggtc
      361 gagcagatgc tgatcttcca cgaccacccc caggacctga gaccggaaca cgagtcggag
      421 tggtcggtgg tccgggaggc gctggccgag gcggcgcgcc ggtcaggctg tccggccatg
      481 ctcgcctcga cccttcccga cctggtctcg gaggagggcc gccggaacct ggccgggacc
      541 ggcatcccgg tcgtcggcgg catgcgggcg gcgatcgcag cggcagcggc caaccagaag
      601 agccggcgca ggccggacga gctggtcacg gagcgactca tggcgatcgc cgcggcaacc
      661 cgcgaggctt caggacctgc ccgcgtcgac cgggctaacg tcggcgaagg caacgacacc
      721 gttggcaacg cggccgatgg ccggtggctg gccgaacacg aagtcaaggc ggtgctgcgg
      781 gaagccggaa tcgccgtccc cgctttcctg accgccggtt ctcccggcgc ctgtctggat
      841 gccgccgccg gcctttcctt cccgctcgtg ctgaagcttt cgggcccgtc cattcagcac
      901 aagtcggaac tgggagcggt tcgggtcggg atcgaagacg aggcgatgct gcttcgccac
      961 gccgaggacc tgatgtcgat cgccgggcag attcatctcg aacagggcct ggccggtgag
     1021 gagtccaccc cgccgccggt ctttctggtc gaggagatgg ccggagcggg ggtcgagatg
     1081 atcgtcaccg cccgctccga cgctgtggtt ccatcgctaa cgatcggcat cggcggcacc
     1141 tgggccgagg acgtgaacga ctccgtggtc atgcccctgc cggtatcccc ggaagacgcc
     1201 ctccgggcgc tgggcagact caaggcattc ggcctgctcc ggccccgcgg cggcgggggc
     1261 gccgacacga tcgccctggc cgagctggcc tcgggcctcg gccggctgct gctcgactac
     1321 cgcgacgggg aaggccgggg actcgacctg atcgaactga atccggttgt ggcagggcag
     1381 gacggggcga cagcgctgga cgcgctggcc catctcgcct gaaccgcggg tcgtggtcag
     1441 atcggccggg tcttgctctg cggcggcagg agcggcggtg ccgcgccgtg gatgcgggag
     1501 gcggtcagct ccagcccctc gcggaggatt gacgcaattt cgtggaagag ctccggctca
     1561 gcgatcagcg gcggggcgat ctggatgatc gggtcggacc ggtcgtcgag gcggcagagc
     1621 agtccccgtt cccagagcgc ctccgagagc acgtccttga agagggtttc cgactcttcg
     1681 ccctggaagg gctccttggt ctcctggtcg cgaaccacct cgatcgccca gaaatggccc
     1741 gtgcccctga cctcgccgac gatcgggatg tcaagcagcg acttgagggc c
//
LOCUS       DAOTIP010000480         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_78276_length_1789_cov_3.152826, whole genome shotgun sequence.
ACCESSION   DAOTIP010000480 DAOTIP010000000
VERSION     DAOTIP010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78276_length_1789_cov_3.152826"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="P5138_08885"
     CDS             <1..1093
                     /locus_tag="P5138_08885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV60733.1"
                     /translation="DRSLAAALASQGADVELITSDFTYGPVPEPQGYEVSLDFYRRLT
                     PRARRLSKAVHHLPEMRKLRRQLDAAPDPMVAHYQWLTMPQIDHRLISRRRPRVMTAH
                     YILPPGPTRKQVSGARKVFGAMDAVIAHSRLGAGRLVEEVGLSEDRVRVIPHGAFDYL
                     TRLPEEKPLPPELEAPPEAPVILFFGLLRPYKGIGVLIRAAEQLAEAPGEATPELWIV
                     GNPRMDLTELMERSERLNVKVRWLPRFIEDPEIPAIMRRADVLVLPYLDGEQSGVLYT
                     GIAFGKAMVVSDVGGIGEVARDHGLARTVEPGNTEQLGAALSGLVIGDNAVEARRELE
                     EKCRAAAEGPFAWEGIARQTIDLYEELLA"
     gene            1090..>1789
                     /locus_tag="P5138_08890"
     CDS             1090..>1789
                     /locus_tag="P5138_08890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV60734.1"
                     /translation="MIVVYILFWLSAGLLFFTHVGYPIALWLLDFVGLGGRAGEVRGR
                     KLSEIPPAVDPDTDEEILPTVSLIIAAYNEETVIESKVKNALELDYPRELLQIIVASD
                     GSSDGTVGIAKEAGADLVLDLPRGGKIAGQNAAAEAATGQLLAFSDANSTWEPGALAE
                     LIEPFRDERVGYVCGQVRFTGPDGGNLEGAYWRYEMRIREMESELAGVTAGNGAIYAV
                     RASAYIPLRPSGSHD"
BASE COUNT          289 a          598 c          605 g          297 t
ORIGIN      
        1 cgaccggtcg ctggccgctg ccctggcatc gcagggggcg gatgtcgagt tgatcacctc
       61 cgatttcacc tacggtccgg tccccgagcc gcagggatac gaagtcagcc tggacttcta
      121 ccgacgcctc actccgcggg cccggcgtct cagcaaggcg gttcatcacc tgcccgagat
      181 gcggaaactc cggcggcagc tcgatgcggc ccctgacccg atggtcgccc actaccagtg
      241 gctgaccatg ccgcagatcg accaccggct gatttcccgc cgccggccca gggtcatgac
      301 cgcccactac atcctgccgc ccggtccgac ccgaaagcag gtctccgggg ccaggaaggt
      361 gttcggggcg atggacgcgg tgatcgccca ttcgcgcctc ggtgcgggga ggctggtcga
      421 ggaggtcggc ctgtccgaag accgggtccg ggtcattccc cacggcgcct ttgactacct
      481 gacccggctg ccggaggaga agccgctgcc gccggagctg gaagctcccc cggaagcccc
      541 ggtcattctc ttcttcgggc tgcttcgccc ctataagggg atcggggtgc tgatccgcgc
      601 ggcggagcag ctggccgaag cgccgggtga ggcaaccccc gaattgtgga tcgtcggcaa
      661 tccccgtatg gacctgaccg aattgatgga gcgctccgag cggctgaacg tcaaggttcg
      721 ctggctgcca cgtttcatcg aggacccgga gatcccggcg atcatgcgcc gggccgatgt
      781 cctcgtgctg ccttacctgg acggcgagca gtcaggggtg ctctacaccg gcatcgcttt
      841 cggcaaggcg atggtcgtct cggacgtcgg cggtatcggc gaggtcgcga gggaccacgg
      901 gctggctcgc accgttgagc ccggcaacac cgaacaactg ggtgcagcgc tgtcggggct
      961 ggtgatcggt gacaacgcgg tcgaggccag gagagaactc gaggagaagt gcagggccgc
     1021 cgccgaggga cccttcgcct gggaaggcat cgcccgccag acgatcgacc tctacgagga
     1081 gctactggcg tgatcgtcgt ctacatcctt ttctggctca gcgcgggcct gctcttcttc
     1141 acccacgtcg gctatccgat tgcgctctgg ctgctcgact tcgtcggtct gggcggtcgt
     1201 gccggcgagg tccggggtcg caagctgagc gagatccctc cggcggtcga tcccgacacc
     1261 gacgaggaga tcctgccgac cgtctcgctg atcatcgccg cctacaacga ggagacggtg
     1321 atcgagtcaa aggtgaagaa cgcactggag ctcgattacc cgcgcgaact cctgcagatc
     1381 atcgtcgcct ccgacggatc ttccgacggc accgtcggga ttgcaaagga agcgggcgcc
     1441 gatctcgttc tggacctgcc gcgtggcgga aagatcgccg gccagaatgc ggcggccgaa
     1501 gcggccaccg ggcagctgct tgccttctcg gatgcgaact cgacctggga gccgggtgcg
     1561 ctcgcggagc tgatcgaacc cttccgggac gaacgggtcg gctatgtttg cggccaggtc
     1621 cgcttcaccg gtcccgacgg cggcaacctc gagggcgcct actggcgcta cgagatgcgg
     1681 atccgcgaga tggagtccga actggccggg gtcaccgccg gcaacggggc gatctacgcg
     1741 gtccgggcct cggcctacat accgctgcgg ccctccggca gccacgacc
//
LOCUS       DAOTIP010000481         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_78743_length_1781_cov_3.527810, whole genome shotgun sequence.
ACCESSION   DAOTIP010000481 DAOTIP010000000
VERSION     DAOTIP010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78743_length_1781_cov_3.527810"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /gene="cimA"
                     /locus_tag="P5138_08895"
     CDS             <1..1495
                     /gene="cimA"
                     /locus_tag="P5138_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRV60735.1"
                     /translation="DQLGVQFIEAGFPASNPKEAELFELLAGEELRQASICAFGMTRR
                     RGVEAEKDEALRLLAATFAPAVTLVGKTWSLHLEKVTKVSREENLEMIGESVAYLVGE
                     GKRVMYDAEHFFDAWGDDRDYSLECLRVAHDSGAEVLVLCDTNGASLPDQVGAAVAEV
                     RSEVPEAELGIHCHNDAECGVANALAAVTAGARQVQGTVNGYGERCGNSNLISILPSL
                     QLKMGYDVVPAENMEELSSVSHFVADLLNVPVDPDQAYVGRNAFAHKGGMHVAGIAAD
                     ARTFEHMDPEAVGNSRSVLPSELSGKATVLARAEEIGLELDEQTARDAVEALKQKEHD
                     GYHYEAAEASFDLLLRREAGQYKPLFTFESFKVQTELRAGERVETEAIVKIRVGDERY
                     VTVAEGNGPVNALDKALRAAITRHYPELDDIELTGYRVRILDTAHGTGAGTRVLLDSR
                     RGEAGDSWGSIGVSPNIIEASWEALVDSLAYAFQNGNSAEGQRAADE"
     gene            1488..>1781
                     /locus_tag="P5138_08900"
     CDS             1488..>1781
                     /locus_tag="P5138_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRV60736.1"
                     /translation="MSEDLPPLDLARPEIGPEEERLVLEVLRSGRLSLGPMLERFEND
                     FAEWLGVDDAVAVSSGTAALHLGVRAFGWGPGDEVVLSPFTFVASANCLLYEGV"
BASE COUNT          316 a          562 c          608 g          295 t
ORIGIN      
        1 cgaccagctc ggggtgcagt tcatcgaagc cggcttcccc gcatccaacc ccaaagaagc
       61 ggagcttttc gaactcctgg ccggcgagga actccgtcag gcatcgatct gcgccttcgg
      121 catgacccgc cggcgcgggg tggaggccga gaaggacgag gccctccggc tcctcgccgc
      181 cacattcgcg cccgctgtaa cccttgtcgg caagacctgg tcgcttcacc tggagaaggt
      241 gaccaaggtc tcccgggagg agaacctgga gatgatcggt gagtcggtcg catacctcgt
      301 aggtgagggc aagcgggtga tgtacgacgc cgagcatttc ttcgacgcct ggggtgatga
      361 ccgcgattac tcgctcgagt gtctgcgggt tgcacacgac tccggggccg aggtgctggt
      421 tctctgtgac accaacgggg ccagcctgcc ggatcaggtc ggcgctgccg tggcggaagt
      481 tcggtcggag gtccccgaag cggagctggg gatccactgc cacaacgacg ccgaatgcgg
      541 tgtggccaac gcgctcgcag ccgttacggc cggggcccgt caggtccagg gcacggtcaa
      601 cggctacggc gagcgctgcg gcaactcgaa cctgatctcg atccttccct cgcttcagct
      661 gaagatgggc tacgacgtgg ttccggcaga gaacatggag gagctttctt cggtctcgca
      721 tttcgtggcc gacctgctca acgttcccgt ggacccggac caggcctacg tcggccgcaa
      781 cgcctttgcc cacaagggcg gaatgcacgt ggccgggatc gccgccgacg cccgcacctt
      841 cgagcacatg gacccggaag cggtcggcaa cagccgctcg gtgcttccct cggaactatc
      901 cggcaaggcg acggtgcttg cccgtgcgga agagatcggg ctcgaactgg acgaacagac
      961 ggcccgggac gcggtcgaag cgctgaagca gaaggagcat gacggctatc actacgaggc
     1021 agccgaggca tcattcgacc tgcttctgcg ccgggaggcc ggccagtaca agccgctgtt
     1081 caccttcgag tcgttcaagg tccagaccga gctgcgggcg ggggaaaggg tcgagaccga
     1141 ggcaatcgtg aagatccggg tcggcgacga gcgttacgtg accgtggccg agggaaacgg
     1201 ccccgtcaac gcgctggaca aggctctgcg ggcagcgatc acccgccatt acccggaact
     1261 ggatgacatc gagctgaccg gctaccgggt ccggatcctc gacaccgccc acggcaccgg
     1321 ggccggaacc agggtcctgc tcgactcgag gcgtggcgag gccggcgatt catgggggtc
     1381 gatcggcgtc tcgccgaaca tcatcgaggc ttcctgggag gcgctggtgg attcgcttgc
     1441 ctacgccttt cagaacggca attctgcgga aggtcagaga gccgccgatg agtgaggatc
     1501 tgcctccgct cgatctcgcc cggccggaaa tcgggccgga ggaggaaagg cttgtactcg
     1561 aagtgctgcg ctctggccgg ctctccctgg gcccgatgct ggagcgcttc gaaaatgact
     1621 tcgccgagtg gctcggagtc gatgacgcgg tcgcggtctc cagcggaacc gcggccctgc
     1681 acctcggagt ccgagcattc ggctgggggc ccggcgacga ggtagtgctc agtccgttca
     1741 ccttcgtggc cagcgccaac tgccttctct acgaaggggt g
//
LOCUS       DAOTIP010000482         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_78872_length_1779_cov_2.550464, whole genome shotgun sequence.
ACCESSION   DAOTIP010000482 DAOTIP010000000
VERSION     DAOTIP010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78872_length_1779_cov_2.550464"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5138_08905"
     CDS             <1..242
                     /locus_tag="P5138_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV60737.1"
                     /translation="VTFESQDEAYRESVLPSSVTKRISIEQASTFGWERYVGEEGVAI
                     GMTTFGASAPFKDLQKYFGFTPDRVVEEAKNLLGD"
     gene            246..>1779
                     /gene="pgi"
                     /locus_tag="P5138_08910"
     CDS             246..>1779
                     /gene="pgi"
                     /locus_tag="P5138_08910"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRV60738.1"
                     /translation="MSELRESEAFKTLESHRADIGDLHLRDLFADDSSRGTDLVAAAC
                     GLYLDYSKNRITRDSVGLLTALARERRMPERRDAMFRGEHINVSEDRAVLHVALRMPR
                     SRSLVVDGVDVVREVHEVLDRMSAFSESVRSGEWKGQTGKPIKRIINIGIGGSDLGPV
                     MAYEALKDYSRRDLEFRFVSNVDGTDFYEATLDADPAETLFVVSSKTFTTLETMTNAH
                     TARQWLLDGLGGDESAVAKHFVAVSTNAEGVAEFGIDTDNMFGFWEWVGGRYSMDSAI
                     GLSTMLAIGPDRFTEMLDGFHEMDEHFLETNLETNLPAIHGLLTVWYRNFFGAETHAV
                     FPYSEYLHRFPAYLQQLTMESNGKSVTLDGTRVDYGTGAIFWGEPGTNGQHSFYQLVH
                     QGTELIPADFIGFNHALNPLGNHQDLLVSNVFAQTEALAFGKTVEQLQAEGVDERLIP
                     HKTFTGNRPTSTIMADRLTPKTLGSLVAFYEHSVFTQGAIWGIDSFDQWGVELGKALA
                     QKI"
BASE COUNT          330 a          594 c          543 g          312 t
ORIGIN      
        1 aagtcacctt cgagagccag gacgaggcct accgggaatc ggtgcttccg tcgtcggtca
       61 cgaagaggat ctcgatcgag caggcctcga ccttcggctg ggagcggtat gtcggcgagg
      121 agggcgtcgc gatcggcatg accacattcg gtgcctcagc cccgttcaag gacctgcaga
      181 aatacttcgg cttcacgccc gaccgggtgg tcgaagaagc gaagaacctc ctcggggact
      241 gagcaatgag cgagctccgg gagtcagaag ccttcaagac cctcgagagc caccgcgctg
      301 acataggtga tcttcacctg cgggatctgt tcgcggacga ttcttcgcgc ggcaccgatc
      361 tggtcgccgc cgcctgcggt ctttacctcg actattcgaa gaaccggatc acccgggaca
      421 gcgtaggcct gctcaccgcg ctggcccgcg agcggcgcat gccggagcgt cgcgacgcga
      481 tgttccgcgg cgagcacatc aacgtctccg aggaccgggc ggtcctccac gtcgccctgc
      541 ggatgccgcg aagccgttca ctggtcgtgg acggcgtcga cgtggtccgc gaggtccacg
      601 aggtgctcga ccgcatgtcg gctttctccg agtcggtccg ctccggtgaa tggaagggcc
      661 agaccggcaa accgatcaag cggatcatca acatcggaat cggcgggtct gacctgggac
      721 cggtcatggc ctacgaggcc ctcaaggact actcgcgccg cgacctcgag ttccgtttcg
      781 tctcgaatgt cgacggcacc gacttctacg aggcgaccct tgacgccgac ccggccgaaa
      841 cgctcttcgt cgtctcctcc aagacattca ccaccctgga gaccatgacc aatgcccaca
      901 cggcccgcca gtggctgctg gacgggctcg gcggcgacga atccgccgtc gccaaacatt
      961 tcgttgcggt atcgaccaac gccgagggtg tcgcggagtt cgggattgac accgacaaca
     1021 tgttcggttt ctgggaatgg gtcggcggcc gctactcgat ggattcggcg atcggcctct
     1081 ccaccatgct ggcgatcggc ccggaccggt tcaccgagat gctcgacggc ttccacgaga
     1141 tggacgagca tttcctggag accaatctcg agaccaacct gccggccatc cacggcctgc
     1201 tgaccgtctg gtacaggaac ttcttcgggg ccgagaccca cgccgtcttc ccctacagcg
     1261 aatacctgca ccgcttcccg gcctacctgc agcagctgac catggagtcg aacggcaagt
     1321 cggtgaccct cgacgggacc cgggtcgatt acggaaccgg cgcgatcttc tggggcgagc
     1381 ccggaaccaa cggccagcat tccttctacc agctggtcca tcaggggacc gagctgatcc
     1441 cggccgattt catcggcttc aaccacgccc tgaatccgct ggggaaccat caggacctgc
     1501 tggtctccaa tgtcttcgcg cagaccgagg cgctggcctt cggcaagacg gtcgagcagc
     1561 ttcaggccga aggggtggac gagcgattga tcccccacaa gaccttcacc ggcaaccgtc
     1621 ccacctcgac catcatggcc gaccgtctca ctccgaagac gctgggttcc ctggtcgcct
     1681 tctacgagca ttcggtcttc acccaggggg cgatctgggg catcgactcc ttcgaccagt
     1741 ggggcgtcga gctgggcaag gcgctggccc agaagatca
//
LOCUS       DAOTIP010000483         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79127_length_1774_cov_3.139034, whole genome shotgun sequence.
ACCESSION   DAOTIP010000483 DAOTIP010000000
VERSION     DAOTIP010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79127_length_1774_cov_3.139034"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="P5138_08915"
     CDS             complement(<1..485)
                     /locus_tag="P5138_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRV60739.1"
                     /translation="MLYDKAKIFVEGGSGGNGCISFRREAHVPRGGPDGGDGGHGGGV
                     LLECDTSRRDLGALRSKHFRAERGHHGEGANKQGARGEDTVIPVPPGTQATSVDGALI
                     DLTEPGQTAVLARGGRGGHGNKRFSTSVRQAPRFAENGTTGESGWVELRLKLLADAGL
                     IG"
     gene            complement(490..1191)
                     /locus_tag="P5138_08920"
     CDS             complement(490..1191)
                     /locus_tag="P5138_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60740.1"
                     /translation="MEEATIFEAALLEGLDEPVRSYLGQAILPGAELTRSWLLRMKGS
                     IKVGLRLPFEARQELDGESFTWRARVPSERIRLLEVADSYRDDDGGMIGRIAGRKVFS
                     DTSPDAARSAATRAVMESTMVPATLLPGRGVEWRVVSGNEVAFRRPGVELAEEVTIRL
                     DGENRAAEVEAPRWLKPKGEDGRLVPFLCRFEGTFESGGMKVPSKLVAGWITDGEFEP
                     FFEADITSVEPISVP"
     gene            complement(1207..1461)
                     /gene="rpmA"
                     /locus_tag="P5138_08925"
     CDS             complement(1207..1461)
                     /gene="rpmA"
                     /locus_tag="P5138_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRV60741.1"
                     /translation="MAHKKGLGSSRNGRDSNPKMLGVKVFAGETVSGGEIIVRQRGTK
                     FWPGEGVGLGRDHTIFATRAGTVEFKPGRRGRTISVTPSE"
     gene            complement(1451..>1774)
                     /locus_tag="P5138_08930"
     CDS             complement(1451..>1774)
                     /locus_tag="P5138_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60742.1"
                     /translation="KGYSRRAGHRAELTRLEVTTLGGGAKKAAPKKAVADKAEEKPKA
                     EAKAETAEKKAPAKKPAAKKPAAKKPAAKAETKTETKAAAKKPAAKKPAAKKPAAKKE
                     ETDGA"
BASE COUNT          258 a          630 c          529 g          357 t
ORIGIN      
        1 ccgatcagac cggcgtcggc gaggagcttg agtcgcaact cgacccagcc ggactcgccg
       61 gtggttccgt tctctgcgaa gcggggtgcc tgccggaccg aggtcgaaaa acgcttgttg
      121 ccatgccctc cacgaccgcc acgagccagt acggcggtct ggccgggttc ggtcaggtcg
      181 atcagggcgc catcgaccga agtcgcctgg gttcccggcg gcaccggaat aaccgtgtcc
      241 tcgccgcgtg ctccctgctt gttcgcccct tcaccgtgat gtccacgctc ggcccgaaag
      301 tgcttggacc tcagggcacc gagatcgcgc cgggaggtgt cgcattcgag caggacgccg
      361 ccgccgtgtc cgccgtcacc gccatcgggc ccaccgcggg gaacatgggc ttcccgccgg
      421 aaggagatgc agccgtttcc accggaaccg ccctccacga aaatctttgc cttgtcgtac
      481 agcaccggcc tagggtacgg agatgggttc gaccgaggtg atgtcagctt cgaagaacgg
      541 ctcgaactcc ccgtcggtga tccaccccgc gaccagcttc gacggcacct tcatccctcc
      601 cgattcgaaa gtgccttcga accggcagag gaagggaacc agtcttccgt cctcgccttt
      661 cggcttcaac cagcgcggtg cctcgacttc ggctgcacgg ttttccccgt ccagcctgat
      721 ggtgacttcc tccgccagct cgacacccgg tcgccggaag gcaacttcat tcccggagac
      781 gacccgccat tcgactcctc tccctggaag cagggtcgcc ggcaccatcg tcgattccat
      841 caccgccctt gttgcggccg accgggcggc atctgggctc gtgtccgaga agaccttccg
      901 gccggcaatt cggccgatca tccccccgtc gtcgtcccgg taggaatcgg ctacctccag
      961 caaccggatc ctttccgatg gaacccgggc ccgccaggtg aacgactcgc catcgagttc
     1021 ctgccgcgct tcgaacggca gccggaggcc gaccttgatc gaacccttca tccgaagaag
     1081 ccagcttcgg gtcagctcgg cgcccggaag gatcgcctgc cccaggtaac tcctgactgg
     1141 ctcgtcgagc ccttccagga gtgccgcttc aaatatcgtg gcttcctcca tgaagccgat
     1201 cggaggctac tccgaagggg tgaccgagat ggtgcggccg cggcggccgg gcttgaattc
     1261 gaccgtgccg gcacgagtgg cgaagatcgt gtgatcacga ccgaggccta cgccctctcc
     1321 gggccagaac ttcgtgccgc gctggcggac gatgatctcg ccaccggaaa cggtttcacc
     1381 ggcgaagacc ttcaccccga gcatcttcgg gttcgagtca cgaccgttcc gtgaggatcc
     1441 gagtcctttc ttatgcgcca tcggtttcct ccttcttcgc ggcaggcttc ttcgccgcag
     1501 gcttcttggc tgccggcttc tttgcggcag ccttggtttc agtcttggtt tcggccttcg
     1561 ccgcaggctt cttggctgcg ggcttcttcg cggccggctt cttcgccggg gccttcttct
     1621 cggcggtttc ggccttggct tccgccttcg gcttctcctc ggccttgtcg gcaacagcct
     1681 tcttcggggc ggccttcttc gcgccgccgc ccagcgtggt cacttcgagc ctggtcagct
     1741 cggcccggtg gcctgcccgc ctgctgtagc cctt
//
LOCUS       DAOTIP010000484         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79240_length_1772_cov_3.061153, whole genome shotgun sequence.
ACCESSION   DAOTIP010000484 DAOTIP010000000
VERSION     DAOTIP010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79240_length_1772_cov_3.061153"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..537
                     /gene="rpsG"
                     /locus_tag="P5138_08935"
     CDS             67..537
                     /gene="rpsG"
                     /locus_tag="P5138_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRV60743.1"
                     /translation="MARRNAAVIRPLDADTKYRSRLVQQIINKVMRDGKKSTAERIVY
                     DALALVGERSGEDAVETLEEAIKQLTPALEVRSRRVGGATYQVPVEVPSRRARTLAVR
                     WLVDFARARNEYSMSERLANEILDATKQQGNAYKRKDDIFRMAQANKAFAHYRW"
     gene            complement(672..1172)
                     /locus_tag="P5138_08940"
     CDS             complement(672..1172)
                     /locus_tag="P5138_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60744.1"
                     /translation="MTGRLAAITTLLGALVVILSQVVTAYSLENELGEILDTVTLLSK
                     HGVITAIIGLIAALATVFAVAAGNRSATIVVIGAGAAVVLVFLFVDVPDIGSTGMFTT
                     QTAGNLDATAKAEAGLWLELLGGVILILGGLALRTLDEEQLRAIGPRIQGGSAARPSA
                     DKEKAS"
     gene            complement(1169..>1772)
                     /locus_tag="P5138_08945"
     CDS             complement(1169..>1772)
                     /locus_tag="P5138_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60745.1"
                     /translation="LVLIAAVAAVLLGVAQFKGLGQIEIGQSGYSGFQDLAKAPAIDH
                     TTPADVHTDVFVPIAVVAFAAALVILLGSVAPSAKRFSSLRRLASMLLVAIGLLTLAV
                     ALLVDLPDATDTAEASLAYSGVEAVLLSGFWLELAAGAALAASGFALLYEPSTGRIGQ
                     RHPESRREGRPRRAEGGSGSGTRAASGSGPAAGPATGSRA"
BASE COUNT          320 a          614 c          584 g          254 t
ORIGIN      
        1 cctcgacgcg gccggtgttt ccgaccgcaa gcaggcccgg tcccgctacg gagtgaaggc
       61 gagctaatgg cccgtcgtaa cgccgcagtc atccgtccgc tcgacgcgga caccaagtac
      121 cgcagccgcc tggtccagca gatcatcaac aaggtgatgc gcgacggcaa gaagtccacc
      181 gccgagcgga tcgtctatga cgccctcgcc ctggtcggcg agcgttcagg tgaggacgca
      241 gtcgagaccc tcgaagaggc gatcaagcag ctcaccccgg ccctcgaggt tcgctcccgc
      301 cgcgtcggtg gcgccaccta ccaggttccg gtcgaggttc cttcccgtcg cgcccgcacc
      361 ctcgcggtcc gctggctcgt cgacttcgcc cgcgcccgca acgagtactc gatgtccgag
      421 cgtctcgcca acgagatcct cgacgccacc aagcagcagg gcaacgccta caagcggaag
      481 gacgacatct tccgcatggc tcaggccaac aaggccttcg cccactaccg ctggtagaag
      541 actcgaacgg ccggttcgcc ggagtcctca gcggcaccca acccgggtgc cacttcttga
      601 gctcggaaaa gcgtgctcgc caagtggcac ctcccaatac gtttcgcgag caccttgatg
      661 aatgccgaac atcaggaggc tttctccttg tcggcggaag gtctggccgc ggatcccccc
      721 tggattcgcg gtccgatcgc gcgcagctgc tcttcgtcga gagtgcgaag ggccaagccg
      781 ccgagaatca ggatcacccc gccgagcaat tcgagccaca ggcccgcctc ggccttggcg
      841 gtcgcgtcca ggttgccggc ggtctgggtg gtgaacatcc cggtgctgcc gatgtccggc
      901 acgtccacga agaggaacac gagcaccacc gccgctccgg cgccgatcac cactatggtc
      961 gccgagcgat ttccggcggc gacggcgaag acggtggcaa gcgcggcgat gagtccgatg
     1021 atcgcggtga tgaccccatg cttggaaagc agggtcaccg tgtcaaggat ctcgcccagt
     1081 tcgttctcga gcgagtaggc ggtgactacc tgggagagaa tcacgaccag cgcccccagg
     1141 agggtggtga tggcggccag ccgcccggtc acgctcgact cccggttgcc ggcccggcgg
     1201 ccggtccgct cccgctcgcg gcgcgcgtcc cggacccgga accgccttcg gcccgccggg
     1261 gtcggccttc tcttcgactc tccggatgcc gctggccgat ccggcccgtt gagggctcgt
     1321 agagcaaagc gaagccggaa gcggccaacg cagcaccggc ggcgagttcg agccagaaac
     1381 cggacagcag gaccgcctcg acgcccgagt aggccagcga cgcctccgct gtgtccgtag
     1441 cgtccggcag gtcgaccagc agggcgaccg ccagggtcag gaggccgatg gcgaccagga
     1501 gcatcgacgc gagccgccgc aggctggaga agcgttttgc cgacggggcg accgagccga
     1561 gcaggatgac caaggcagcc gcgaaagcaa caacggcgat cgggacgaag acgtcggtgt
     1621 gcacatcggc cggtgtcgtg tggtcgatcg ccggcgcctt ggcgaggtcc tggaatccgg
     1681 aatagccgga ctgtccgatc tcgatctggc caaggccctt gaactgggcg acacccagca
     1741 ggaccgcggc gacggccgcg atcaggacca gg
//
LOCUS       DAOTIP010000485         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79249_length_1772_cov_2.692487, whole genome shotgun sequence.
ACCESSION   DAOTIP010000485 DAOTIP010000000
VERSION     DAOTIP010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79249_length_1772_cov_2.692487"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..>1772)
                     /locus_tag="P5138_08950"
     CDS             complement(48..>1772)
                     /locus_tag="P5138_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HRV60746.1"
                     /translation="TSMTINAVAAWMLGLYVAAAENTGVDRAQLKGTTQNDIIKEYLS
                     RGTYAFPPEPSMRLIADMVAFTVNEIPSWNPINICSYHLQEAGATPVQEIAYAMSTAI
                     AVLDEVKARGQVSDEDFPKVFGRISYFVNAGVRFIEEIAKLKAMGRLWTEIGRERYGV
                     TDEKMLRFRYGVQVNSLGLTEAQPENNIQRIVVEALAVTLARDARARALQLPAWNEAM
                     GLPRPWDQQWSLRIQQVLAFETDMLEYPDIFEGSVVMEGLVEEFMSGARDEMAVVAEK
                     GGAVEAVPYMKARLVESHRERFARIENGEQTVVGLNKYTETEDSPLTASADGGIMKPD
                     PQVEKERIEALDQWRSNRDQAAVDEALAEVARIAGTDENIMPATIAAAKAGATTGEWA
                     ATLREVFGAYRGPTGVGGAAPAGDRDVLTDVRAKVDEVSEQIGHRIRLLVGKPGLDGH
                     SNGSEQIALRARDVGMEVVYQGIRLTPEQIVESAVQEDVDLIGLSILSGSHLELIPEV
                     VRLLKEEGVEVPVVAGGIIPPEDAARLKEAGVAGVFTPKDFDMNEIMSEFVDLVAAQH
                     EDSAAAPA"
BASE COUNT          269 a          594 c          584 g          325 t
ORIGIN      
        1 ccgtgagtca gcaggtgaag ctgatcactc acggggccag gaacctccta agcgggcgcg
       61 gccgccgagt cctcgtgttg tgctgcgacc agatcgacga attcgctcat gatctcgttc
      121 atgtcgaagt ccttcggggt gaagactccg gccacgccgg cctccttcag gcgggcggcg
      181 tcctccggcg ggatgatgcc accggcaacc accggaacct ctacgccttc ctccttcagc
      241 aggcgcacga cctccggaat aagctccaga tgggacccgg agaggattga aagaccgatc
      301 aggtccacgt cctcctggac cgccgattcg acgatctgct ccggagtcag gcggatgccc
      361 tggtagacga cctccatacc cacgtcacgg gcccgcagag cgatctgctc ggagccgttc
      421 gagtggccgt cgagaccggg ctttccgacc agcaggcgga tccggtggcc gatctgttcg
      481 gacacctcgt ccaccttcgc ccggacgtcg gtcagtacgt cgcggtcgcc ggcaggggcg
      541 gcgccgccga caccggtcgg tccgcggtag gcgccaaaga cttcgcggag ggtggccgcc
      601 cactcgcccg tggtcgcgcc ggccttggcg gcggcaatcg tcgcgggcat gatgttctcg
      661 tcggtcccgg cgatccgggc gacctccgcc agggcctcgt cgaccgcggc ctggtcccgg
      721 ttcgagcgcc actggtcgag cgcctcgatc cgttccttct cgacctgcgg gtcgggcttc
      781 atgatgccgc cgtcggcact cgcggtcagc ggtgaatcct cggtctcggt gtacttgttg
      841 agcccaacga cggtctgctc accgttctcg atccgggcga agcgttcgcg atgcgactcg
      901 accaggcggg ccttcatgta tggcaccgct tcgaccgcgc cacccttttc ggcgaccacg
      961 gccatttcgt cccgggcgcc cgacatgaac tcttcgacca ggccctccat gacgaccgag
     1021 ccctcgaaga tgtccgggta ttcgagcatg tccgtctcga aagccaggac ctgctggatg
     1081 cggaggctcc actgctggtc ccaggggcgg ggcaggccca tcgcctcgtt ccaggccggc
     1141 agctgcagtg cgcgggcccg ggcgtcgcgg gccagggtca cggcgagcgc ctcgaccacg
     1201 atccgctgga tgttgttctc cggctgggct tcggtcaggc cgagcgagtt gacctggacg
     1261 ccgtagcgga agcgcagcat cttctcgtcg gtaacgccgt aacgctcgcg gccgatctcg
     1321 gtccagagac gtcccattgc cttcagcttg gcgatttcct cgatgaaccg gacgccggcg
     1381 ttgacgaagt aggagatgcg cccgaagacc ttggggaagt cctcgtcgga aacctggcca
     1441 cgggccttga cctcatccag cacggcgatc gccgtcgaca tcgcatatgc gatctcctgg
     1501 actggggtcg ctcccgcttc ctgcaggtgg tagctgcaga tgttgatcgg attccagctc
     1561 gggatctcgt tcacggtgaa ggcgaccatg tcggcgatca gccgcatcga gggctccggc
     1621 gggaaggcgt aggtgccgcg ggagaggtac tccttgatga tgtcgttctg ggtggtgccc
     1681 ttgagctggg cccggtccac gcccgtgttc tcggcggcgg cgacatagag gccgagcatc
     1741 caggcggcga ccgcattgat cgtcatcgac gt
//
LOCUS       DAOTIP010000486         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79298_length_1771_cov_3.253497, whole genome shotgun sequence.
ACCESSION   DAOTIP010000486 DAOTIP010000000
VERSION     DAOTIP010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79298_length_1771_cov_3.253497"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="P5138_08955"
     CDS             complement(<1..825)
                     /locus_tag="P5138_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV60747.1"
                     /translation="MSDKRDYYETLGVSREATDTEIKKAFRKVARELHPDVNAHDPQA
                     EEKFKEAAEAYEVLSDQERRDTYDQFGHDGLRSGGYQSQAQGFGSIDDLFSAFFGGGF
                     GGNRGPAPGADIGVQTEIELEDVLDGTAREVEFEAVSNCEHCHGNGAEPGTPIKTCER
                     CGGSGELRSVANTAFGQMVRSTPCDQCHGDGRIAEEPCKECSGLGRKHAARKHRIDIP
                     AGIESGQRIRIAGAGHAGETGAPPGDLYVEVHVAEREGMVRDGQDLITVIPVDATAA"
     gene            complement(822..>1771)
                     /locus_tag="P5138_08960"
     CDS             complement(822..>1771)
                     /locus_tag="P5138_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HRV60748.1"
                     /translation="KTIAGDSRLEWGASTVRAELAALEAGGYLTHPHTSAGRVPTDLG
                     YRQYVESLKSRAVARPGDAGTLGLSDLRREVDEAMQEATSALAQMTDLLALVVAPPLG
                     TAKIHRVEALLLQPEVVMVIVITSAGDVTRRAFTFPGSVDPGLVEWASSYLNESLSGI
                     DLGARRIASKLADPALGPVEAAFVEQLAPALVVLEQSPEGTLYVEGASRLLDQRRAAD
                     LPRIDRLMNTLEEKATVLRMMRSAIDERSVFLWIGAENPQPELQDVTVIGANYGLAHR
                     NLGSVGVVGPTRMDYERAIASVSEASRELSRYFEFVYDA"
BASE COUNT          269 a          592 c          595 g          315 t
ORIGIN      
        1 cgccgccgta gcgtccaccg ggatgaccgt gatcaggtcc tgcccgtccc ggaccattcc
       61 ctcgcgttcg gccacatgga cctcgacata gaggtcaccg ggcggcgccc cggtctcccc
      121 cgcgtggccg gcgcccgcta tgcggatcct ctggccggat tcgatcccgg ccggtatgtc
      181 gatgcggtgc ttccgggctg cgtgcttgcg gccgagtccg gagcattctt tgcacggttc
      241 ttccgcgatc cggccatcgc cgtggcactg gtcacagggt gtcgaacgga ccatctgtcc
      301 gaaagctgtg ttggccacgg acctgagctc gccgctgccg ccgcagcgct cgcaggtctt
      361 gatcggggtg cccggctcgg cgccgttgcc gtggcagtgt tcgcagttgg agaccgcctc
      421 gaattcgacc tcgcgggcgg tgccgtcaag cacgtcctcg agttcgatct cggtctggac
      481 cccgatgtcg gcgccggggg ccgggcccct gtttccgccg aagcccccgc cgaaaaaggc
      541 gctgaagaga tcgtcgatcg acccgaaccc ctgggcctgg gactggtacc cgccggagcg
      601 aagtccgtca tgcccgaact ggtcataggt gtcgcggcgc tcctggtcgg agagcacttc
      661 gtaagcctcg gctgcttcct tgaacttctc ttcggcttgc ggatcgtggg cgttcacatc
      721 cggatggagt tcacgggcca ccttgcggaa agccttcttg atctcggtgt cagtggcctc
      781 gcgggagacc ccgagggttt cgtagtagtc gcgtttgtcg ctcatgcgtc gtagacgaac
      841 tcaaagtagc gggagagctc acgcgatgcc tcgctgacgc tggcgatcgc ccgctcgtag
      901 tccatgcggg tcggaccgac cacaccgacc gatcccaggt tgcggtgggc gagcccgtag
      961 ttggcgccga tcacggtcac gtcctggagt tcgggctgcg ggttttcggc gccgatccag
     1021 aggaagaccg agcgctcgtc gatcgccgag cgcatcatgc gaaggaccgt ggccttttcc
     1081 tcaagggtgt tcatcaggcg gtcaatccgg ggcaggtcgg cggcacgacg ctggtcgagg
     1141 aggcgcgatg ccccttccac atagagggtg ccttccgggc tctgctcgag gacgaccagg
     1201 gccggggcca attgctcgac aaaggccgcc tccaccggac cgagcgccgg gtctgccagt
     1261 ttcgaggcga tcctccgtgc cccgaggtcg atcccggaaa gcgactcgtt caggtagctg
     1321 gaggcccatt cgaccagacc gggatcgacc gacccgggaa aagtgaaggc gcgccgggtg
     1381 acgtcgccgg ccgaggtgat gacgataacc atcaccactt ccggctggag cagcagcgcc
     1441 tcgacccggt ggatcttcgc cgtgccaagc ggcggcgcga cgaccagtgc gagcaggtct
     1501 gtcatctgtg cgagagccga ggtggcttcc tgcatcgcct cgtcgacttc ccggcgcagg
     1561 tcgctcaagc cgagggtgcc ggcgtccccc gggcgggcaa ccgcacgtga cttgagcgat
     1621 tcaacgtact gccggtaccc gagatcggtc ggaacccgcc cggccgaggt atgcggatgg
     1681 gtcaggtacc cgccggcctc aagcgcagcc agttccgccc ggacggtgga agcaccccac
     1741 tccagccgcg aatccccggc gatcgtcttg g
//
LOCUS       DAOTIP010000487         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79353_length_1770_cov_5.664723, whole genome shotgun sequence.
ACCESSION   DAOTIP010000487 DAOTIP010000000
VERSION     DAOTIP010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79353_length_1770_cov_5.664723"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..463
                     /locus_tag="P5138_08965"
     CDS             107..463
                     /locus_tag="P5138_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRV60749.1"
                     /translation="MAHRSRLAGFIIDSYRYDPDLMKVIIVEVTRAANSFGRTHLDEI
                     NRAYELIGRIVADAQQKAIFREDIDPAFAAMVFYGAIEQLLSGWIFGVIPGSDRDFDE
                     ARDLLVKAICEGLQRR"
     gene            507..641
                     /locus_tag="P5138_08970"
     CDS             507..641
                     /locus_tag="P5138_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60750.1"
                     /translation="MDNDIVRRITWSIMLTASSALASLIASRLAAIAYRRFFNEEPPE
                     "
     gene            738..1106
                     /locus_tag="P5138_08975"
     CDS             738..1106
                     /locus_tag="P5138_08975"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRV60751.1"
                     /translation="MVFEVSERTSNLIREEIELAKTEVSEKVTTLARGSAVGIAAGIF
                     AFLALILLMNAFALGVSSLFGWGFWAGYLLEGVIFIAIAAGAGYFAYNSFEKTGAPIP
                     TEAIEEAQKTKALLTPGDES"
     gene            1106..1486
                     /locus_tag="P5138_08980"
     CDS             1106..1486
                     /locus_tag="P5138_08980"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HRV60752.1"
                     /translation="MSEEKPEAGEPAEGVEKPFRPEGVGPDAPVEKTPNRHVAPEGTP
                     APAGGTPGSPYAAPRPKRNSSQIRADIDAQRAELGQSVDQLRDRVTELTDWKSQLRKH
                     RKQIIVGAVATGFVVGGLMALRRR"
BASE COUNT          317 a          627 c          532 g          294 t
ORIGIN      
        1 ccgacaccta catccgcgat ggcgatggct ggaaggtggt gcaggtccag accacgcgca
       61 tccggccatc gccctgatcc gccgtcttcc cgcaccgggg acctgcatgg cacaccgtag
      121 ccgcctcgcc ggattcatca tcgactccta ccgctacgac cctgacctca tgaaggtgat
      181 catcgttgag gtgacccgcg cagcgaactc cttcggccgc acccatctcg acgagatcaa
      241 ccgggcatac gaattgattg gccggatagt cgccgacgcc cagcagaaag cgatcttccg
      301 cgaggacatc gacccggcct ttgccgcaat ggtcttctac ggggcgatcg aacaactcct
      361 ttccggctgg atcttcgggg tgatcccggg tagtgaccgg gacttcgacg aggcccgcga
      421 cctcctggtc aaggcaattt gcgaaggcct gcagagacgc tgacggaatt tagttctccg
      481 ttggcttggg tattgtggaa ctaccaatgg ataacgacat cgtcagacga attacctgga
      541 gcatcatgct caccgccagt agcgccctgg cgagcctgat cgcttcccgg ctcgccgcta
      601 ttgcctaccg ccgtttcttc aacgaggaac cgcccgagta gatcgtggcc cctcaggact
      661 accagccgcc gcctcgccag cgaccgcccg gccggcccga cccggaccgt cccgcgtccg
      721 agaagacggt gggcgaaatg gtcttcgagg tctccgagcg cacctcgaac ctgatccgcg
      781 aggagatcga actcgccaag accgaggtca gcgagaaggt caccaccctc gctcgtggct
      841 ccgcggtcgg gatcgcggcc ggcatcttcg ccttcctggc cctgatcctg ctgatgaacg
      901 cgttcgcgct cggggtctcg tcactgttcg gctggggatt ctgggcgggg tacctgctcg
      961 agggcgtgat cttcattgcg atcgccgccg gcgccggata tttcgcctac aactccttcg
     1021 agaagaccgg cgcaccgatc ccgaccgagg cgatcgagga agcccagaag accaaggccc
     1081 tgctcacccc cggagatgaa tcctgatgag cgaagagaaa ccagaagccg gcgagccagc
     1141 ggagggagtg gagaagcctt tccgccccga aggtgtcggc cccgacgctc cggtcgaaaa
     1201 gacccccaac cgccatgtcg ctcccgaggg cacaccggcc ccggcgggcg ggacccccgg
     1261 ctcgccatac gccgcgccgc gccccaagcg caactcgtcc cagattcgcg ccgacatcga
     1321 cgcccagcgg gccgagctag gccagtcggt cgaccagctg cgtgaccggg tcaccgaact
     1381 caccgactgg aagagccagc tccgcaagca ccgcaagcag atcatcgtcg gagccgtcgc
     1441 gactggcttc gtggtcggag gcctgatggc cctccgccgc cgttaatccg ggcaacgcgc
     1501 ccaggaacca ccagccgcac gctgaccttc gtcggtcgct cgacggttcg ggaacgtact
     1561 tccagtacgc ccccttcacc gccttcgctt gcctcctcgg cagcgcacgg ctggaggccc
     1621 ctgtgcacga cccgctcacg gtaggtgccg cctcggcagc cccgccctgg aggcccctgg
     1681 catccacccg ctgaaggtcg ggcccggcag cgcaaggtgt aacaagtttt gtgcgttgtc
     1741 tgcgggaaat gtgtttcgcc tccgctggcc
//
LOCUS       DAOTIP010000488         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79375_length_1770_cov_3.006997, whole genome shotgun sequence.
ACCESSION   DAOTIP010000488 DAOTIP010000000
VERSION     DAOTIP010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79375_length_1770_cov_3.006997"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..690
                     /locus_tag="P5138_08985"
     CDS             37..690
                     /locus_tag="P5138_08985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60753.1"
                     /translation="MPEYSPSADRKTVVVGKAICRLVAVAPWAWPLVGSSVGRFFDHA
                     AAGWDERTSAGSAGHLATLAAGLDSLQSKPERVLDLGCGTGAGTLFLSREFPTASIRG
                     LDLSESMIRQAQGKIGLDPEGRVNFRVGDASGLPFSDDSFDLVTQVNLPIFFREIARV
                     LRPGGGLVIASSHGDQTPFSTPESLVFRKLASRGFGSIGSGTAGEGTWISARLEEQS"
     gene            687..1580
                     /locus_tag="P5138_08990"
     CDS             687..1580
                     /locus_tag="P5138_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRV60754.1"
                     /translation="MTEERAYTLILNPSSGGGRSKQVLPEVERRLDALRIPFRVERTR
                     SLEHGVDLAFEAVDSREIPVVVSGDGLIGAVGGALAGVDSPVGLIPAGRGNDLARGLG
                     IPTDPAAAVDCLAAGFTRSIDVGDANGERFLGIASVGFDSDANRIANQAKLLKGQPVY
                     AYAALRALLRWKPARFTLIESGVQSRITGYTVIVANNGFYGGGMNVAPTAEIDDGKLD
                     LVTIGEVGKFRFLLNMPKVFKGTHVNEAETVSTHRVTSIEVRASRPFTVYADGDPLTE
                     LPATIRVIPSALRMIVPEPAS"
     gene            1577..>1770
                     /locus_tag="P5138_08995"
     CDS             1577..>1770
                     /locus_tag="P5138_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HRV60755.1"
                     /translation="MIRSKAWIARSVGRLSRASGRGGGTTLPGRVLLKLDADAIDKLG
                     AGLSDGATLISATNGKTTTAS"
BASE COUNT          292 a          598 c          581 g          299 t
ORIGIN      
        1 gccagtttat ggcgttccct ctgaaatgat ccgcaggtgc ccgagtacag cccatccgcc
       61 gaccggaaga cggttgtcgt cggcaaggcg atctgtcgcc tcgttgccgt cgctccctgg
      121 gcgtggccgc tggtcggcag ctcggtcggc cggtttttcg accatgccgc cgccggctgg
      181 gacgagcgga catcggccgg gtcggccggt cacctcgcca cgctggcggc aggactcgac
      241 tcgcttcagt cgaagccgga gcgcgtgctc gaccttggtt gcggaaccgg cgccggaacc
      301 ctgtttctct cccgcgaatt cccgaccgcc tcgatccggg gtctcgacct gtcggaatcg
      361 atgatcagac aggcccaggg gaagatcgga ctcgaccccg agggcagggt gaacttccgg
      421 gtgggcgacg cctcgggact tcccttctcc gacgattcct tcgacctcgt cacccaggtc
      481 aacttgccga tcttcttccg cgagatagcg agggtcctca ggccgggtgg cggcctcgtg
      541 atcgccagca gccatggcga tcaaaccccg ttcagcacgc ccgagagcct cgttttcagg
      601 aaactggcgt cccggggctt cggatcgatc ggctccggca ccgccggtga aggaacatgg
      661 atctcggcga gactggaaga gcagagttga ccgaggaacg cgcctacacc ctgatcctga
      721 atccgagctc cggcggcgga cgctcgaagc aggtcctgcc ggaggtcgaa cgccggctcg
      781 atgcacttcg aatccccttc cgggtcgaaa ggacccgttc gctcgagcat ggagtcgatc
      841 tcgccttcga agcagttgat tcccgggaga tcccggtggt ggtcagcggc gacgggttga
      901 tcggcgcggt cgggggcgca ctggcgggtg tagacagccc ggtcgggctg atccctgcgg
      961 gccgcggcaa cgacctcgcc aggggcctcg gaataccgac agacccggcg gccgcggtcg
     1021 actgtctggc ggccgggttc acccgctcga tcgacgtcgg tgatgccaac ggtgagcgtt
     1081 tcctcggcat cgcctcggtc ggtttcgact ctgacgccaa ccgcatcgcc aatcaggcga
     1141 agctgctgaa aggccagccc gtctacgcct acgcggccct tcgggccctg ctgcgctgga
     1201 agccggcccg attcacgctg atcgagagcg gtgtccagag ccggatcacc gggtacacgg
     1261 tgatcgtcgc caacaatggc ttctacggcg ggggcatgaa cgtcgccccg accgccgaga
     1321 tcgacgacgg aaaactggac ctggtgacga tcggcgaggt cggcaagttc cgtttcctgc
     1381 tcaacatgcc gaaagtcttc aagggcaccc acgtcaacga agccgagacc gtttcaaccc
     1441 accgcgttac ctcgatcgag gtccgggcca gccgtccgtt caccgtctat gcggacggcg
     1501 acccgctgac cgaactcccc gcgaccatca gggtgatccc ctccgcgctg cggatgatcg
     1561 tgccggagcc ggcctcttga tcaggtccaa ggcctggatc gcccggtcgg tcggccggct
     1621 cagccgcgcg tccggtcgag gcggcggaac caccctgccg gggcgggtcc tgctcaagct
     1681 cgatgcagat gcgatcgaca agctgggtgc cgggctgagc gacggcgcca ccttgatcag
     1741 cgccaccaac ggcaagacga cgactgcgtc
//
LOCUS       DAOTIP010000489         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79717_length_1765_cov_2.706433, whole genome shotgun sequence.
ACCESSION   DAOTIP010000489 DAOTIP010000000
VERSION     DAOTIP010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79717_length_1765_cov_2.706433"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="P5138_09000"
     CDS             complement(<1..554)
                     /locus_tag="P5138_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60756.1"
                     /translation="MKRFLGAVALFIVAVVPSAATAHQGNPDFRSEITSVQPAALGNG
                     LQIEIVNFDDHVRLVNKSGKEVVIKGYDGEPYARISPDGTVSVNLNAPAYYLNEDRYA
                     NVDVPARADANATPDWKVIDDNGTFEWHDHRSHYMSQGTPPQVKDKSEKTKVFDYTIP
                     IEVGGQPAKIDGTLTWVGTGSKAPV"
     gene            complement(645..959)
                     /locus_tag="P5138_09005"
     CDS             complement(645..959)
                     /locus_tag="P5138_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60757.1"
                     /translation="METGRTVSLPRTAGLLAVTVVWALLLALSGSPEAILFTIPVFLI
                     AAPLAVERYAGEQLILAMRSGNPRRGNRIPKLALPIRVEISDGIIRAGRLSGRGPPLA
                     SH"
     gene            1013..>1765
                     /locus_tag="P5138_09010"
     CDS             1013..>1765
                     /locus_tag="P5138_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60758.1"
                     /translation="MAQIKALLFNDPSCPWGYSASPTLRTLEWRYRDQIEWHLAVIGL
                     SDENHPVRLSPEQMSDRYFDFRCRYGMPFAIETKIRKFTSATACKVITAARLLYPGSE
                     WRVLRTLQLLHFNTPLILDDRRQLGEALETVPGIEAEVVMGAVDNPEVVSAYEADYAH
                     ARSATGTATHLQHKTAPYSAGERYTAPSVIFDYGERRVEVGGFQPVEAYDVMIANLDP
                     GLKRVGHAEDPLEVLALYPAGLTTQEVATIMTG"
BASE COUNT          329 a          553 c          555 g          328 t
ORIGIN      
        1 accggggcct tcgatccggt acctacccag gtcagggtgc cgtcgatctt cgctggctgg
       61 ccgccgacct cgatcgggat cgtgtaatcg aagaccttcg tcttctcgga cttgtccttc
      121 acctgcggcg gcgttccctg tgacatgtag tgggaacggt ggtcatgcca ctcaaaggtg
      181 ccgttgtcat cgatcacctt ccagtccggg gtcgcattcg catcggccct ggcgggtacg
      241 tcgacgttgg cgtagcggtc ctcgttcagg tagtaggcgg gtgcgttcag gttcaccgaa
      301 acggttccgt ccggggagat ccgcgcgtaa ggctcgccgt catagccctt gatgacgacc
      361 tccttgcccg acttgttgac caggcggacg tgatcgtcaa agttgacgat ttcaatctgc
      421 agaccgttgc cgagggcggc cggctggacc gaggtaatct ccgaccggaa atcagggttg
      481 ccctgatggg cagttgcggc cgaggggacc actgcgacga taaacagggc gacagcaccc
      541 aggaaacgct tcatcaaatt ctccaatgcc accggttgtg gctcgctagt acttgtggga
      601 ttgacgggtc ggtctcggtg gattccggtc cgtcggggat cggttcagtg actggcgaga
      661 ggcggtccac gaccagaaag gcggccggcc cggatgatcc cgtcgctgat ttcaacccgg
      721 atcggcaaag cgagtttcgg gatgcgattg ccgcgtcggg gattcccgct ccgcatcgca
      781 aggatcaatt gctcaccggc gtagcgctca acggccagcg gagcggcgat caggaagacc
      841 ggaatcgtga acagaatggc ttccggggag cctgaaagcg ccagaagaag cgcccagaca
      901 accgtcaccg cgagcagtcc ggcggttctg ggcagcgaga ctgttcggcc cgtctccatc
      961 agttggagtc tagccgggat gcccgcgacc acgaccatct aggcttcggc acatggcgca
     1021 gatcaaggca ctcctgttca acgacccgtc ctgcccatgg ggctattccg caagtcctac
     1081 cttgcggacc ctggagtggc gataccggga ccagatcgaa tggcatctgg cggtcatcgg
     1141 cctctccgac gagaatcatc cggtccgcct ctcacccgag cagatgtcgg atcgctactt
     1201 cgatttccgt tgccgctacg ggatgccgtt cgcaatcgaa acgaagatca ggaagttcac
     1261 cagcgccacc gcctgcaagg tgatcacggc ggcccggctg ctctatccgg gctcggagtg
     1321 gcgggtgctg aggaccctgc agttgctcca tttcaacacg ccgctgatcc tcgacgaccg
     1381 tagacagctg ggcgaagcgc tggaaaccgt tcccggaatc gaagcggagg tggtcatggg
     1441 cgccgtggac aacccggaag tcgtatccgc ctacgaagcc gactacgcac atgcccgcag
     1501 cgccaccggt accgccaccc acctccagca caagacggcc ccctactcgg ccggggagcg
     1561 atacacggct ccctctgtga ttttcgacta cggcgaacgc cgggtcgagg tcgggggctt
     1621 ccagccggtc gaggcctatg acgtgatgat cgcgaacctc gatcccggtc tgaaacgggt
     1681 cggacacgcc gaggatcctc tcgaggtgct tgccctgtac ccggccggac tgaccaccca
     1741 ggaggtcgcg acgatcatga ccggc
//
LOCUS       DAOTIP010000490         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_79943_length_1761_cov_3.970106, whole genome shotgun sequence.
ACCESSION   DAOTIP010000490 DAOTIP010000000
VERSION     DAOTIP010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79943_length_1761_cov_3.970106"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="P5138_09015"
     CDS             complement(<1..353)
                     /locus_tag="P5138_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60759.1"
                     /translation="MRGTWVTAAALMAACAALAGCGSSTHENEPRPPVPTVVSVSVGE
                     DEISANAAIGRGVGEPGARQPYLNQNRNAPQNQADRAAPAVVNVAVANLTDRATTLRM
                     QGPVSRVLALTPGGSG"
     gene            complement(382..1440)
                     /locus_tag="P5138_09020"
     CDS             complement(382..1440)
                     /locus_tag="P5138_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV60760.1"
                     /translation="MSEARHQGLGGGKLGADSPLFGGPGDELNGNGAAHGTVRDPDEA
                     PAWDPHQDDFADDFADSREAAAEEANRAAEELLKLVDLRDLLPASEPDRALDDWGRSE
                     TVVSLMEPILNFYYRYWFRVEVEGIENIPSEGGALIVSNHSGALPPDAPMIMQAIRNE
                     HPAPRPLYMLGEHWFKGYPGVSMLVNKMGLVPAHRENAQRLLHDEGRLAVVFPEGQKG
                     TRKVYWQRYHLRRFGRGGFVRTAIRARVPIVPTAVIGAEESAPIFAHFKLLQKLSGLI
                     YFPLTPSFPHFGLIAPLMYLPSKFKIRFMEPVDMAEYPVETIDDPAEIQVISERLRAR
                     IQHQLDDMLAARESVWTG"
     gene            complement(1437..>1761)
                     /locus_tag="P5138_09025"
     CDS             complement(1437..>1761)
                     /locus_tag="P5138_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRV60761.1"
                     /translation="VERAVSFTKGCYIGQEPVARLHYKGRPNRHLRGLKLAGPVSPGD
                     PVQLDEKPLGEIGTAVLSPASGRIALAILRKEAEPGSTVSVETGEGPVEAQVVELPFV
                     EGFAT"
BASE COUNT          283 a          585 c          585 g          308 t
ORIGIN      
        1 ccggagccgc ccggggttag ggcgagaacc cggctcaccg gaccctgcat gcggagggtc
       61 gtggcccggt cggtcagatt ggcgaccgcg acgttgacca ctgcgggcgc ggcgcgatca
      121 gcctggttct gcggcgcgtt ccggttctgg ttcaggtagg gctgacgggc gcccggctcg
      181 ccgactcctc gcccgattgc cgcattggcg ctgatctcgt cctcaccgac ggaaacgctg
      241 accacggtcg ggaccggcgg tctgggttcg ttctcatggg tggaggagcc acaaccagca
      301 agtgcggcgc atgcagccat cagggctgcg gctgtgaccc aggttcccct cacggcccga
      361 aatgtatccc acctcgtggc gtcaaccggt ccacaccgac tcgcgggccg cgagcatgtc
      421 atcgagctga tgctggatcc gggcccggag ccgttcgctg atcacctgga tctcggccgg
      481 atcgtcgatc gtctcgaccg ggtattccgc catgtcgacc ggctccatga agcggatctt
      541 gaacttggaa ggcaggtaca tgagcggcgc gatcagcccg aaatggggaa acgagggggt
      601 gagcgggaag tagatcagcc cggagagttt ctggagcagc ttgaaatggg cgaatatcgg
      661 tgccgattcc tccgcgccga tcaccgccgt cgggacgatc gggacccggg ccctgatcgc
      721 ggtccggacg aaaccacccc ggccgaaccg ccgcaggtgg tagcgctgcc agtagacctt
      781 gcgggttccc ttctggcctt ccggaaagac caccgcgaga cgaccctcgt catggagcag
      841 gcgctgagcg ttttcccggt gggccggaac gagtcccatc ttgttgacca gcatcgagac
      901 tcccggatac cccttgaacc agtgctcgcc gagcatgtag agagggcgtg gggcggggtg
      961 ctcgttacgg atcgcctgca tgatcatcgg ggcgtcaggg gggagggcgc cggaatggtt
     1021 ggagacgatc agggcgccgc cctcggaggg gatgttctcg atcccctcga cctcgacccg
     1081 gaaccagtag cggtaataga agttcaggat cggttccatc agggaaacca cggtttccga
     1141 gcggccccag tcatcgagcg cccgatccgg ttccgaggcc ggaagcaggt cccgcaaatc
     1201 aaccagcttc aggagttcct ccgcagcccg gttcgcctcc tcggctgccg cttccctcga
     1261 gtcggcgaaa tcgtcggcga aatcatcctg atggggatcc caggccgggg cctcgtccgg
     1321 gtcgcggacc gttccatggg cggccccgtt tccgttcagc tcgtcgccgg gtccgccgaa
     1381 aagcgggctg tccgctccca gcttgccgcc cccgagtccc tgatggcgag cttcgctcat
     1441 gttgcgaatc cttccacgaa gggaagttcc accacctgcg cctcgaccgg gccctcgccc
     1501 gtttcgaccg agactgtcga tcccggttct gcctccttgc ggaggatcgc cagggcgatc
     1561 cggccggatg ccggtgaaag caccgcggtt ccgatctcgc ccagcggctt ctcatccagc
     1621 tgcacagggt cacccgggct caccgggccc gcgagcttca gcccgcggag atgccggttg
     1681 ggccgcccct tgtagtggag ccgggcaacc ggttcctggc cgatgtagca gcccttggtg
     1741 aagctgaccg cccgttccac g
//
LOCUS       DAOTIP010000491         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_80154_length_1758_cov_3.132707, whole genome shotgun sequence.
ACCESSION   DAOTIP010000491 DAOTIP010000000
VERSION     DAOTIP010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80154_length_1758_cov_3.132707"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="P5138_09030"
     CDS             <1..487
                     /locus_tag="P5138_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60762.1"
                     /translation="LAFEIEKERRHAIELEPDLAGELEELDQELPERSEAGEAFSALS
                     SSAAELDDAILAQTTMQLFATDDFDHGPGPEKSLAPAPAARKPLKSLAPKSDSAYAVR
                     ERLREERKELVADVSRRTGESYQQINARINQVLGVASVSKATREELERGNALLERDLK
                     I"
     gene            623..1225
                     /locus_tag="P5138_09035"
     CDS             623..1225
                     /locus_tag="P5138_09035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRV60763.1"
                     /translation="MYAAQSLPGPLEVSAAAPLPEGSRTALLVVDMQNDFVEEGAPRQ
                     VHGALEIVPAIASAVERMRIQGDLIVWTCRAYSADGSNVEAARKPGFKKNPHTVAGTR
                     GAEIVDGLTPWPNDLNVTTPSYSAFFRTDLDLKLREAGVSKIVGCGVDLSRCVRATLV
                     EAISLGYECSVLAPAVASRDDQALNQNLADLADLDITVEE"
     gene            complement(1226..>1758)
                     /locus_tag="P5138_09040"
     CDS             complement(1226..>1758)
                     /locus_tag="P5138_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60764.1"
                     /translation="IDYLEAAWQDALQGRPAASPYIEAEFPSSIDDSLTDDDRVIMTM
                     FTQYGPSEESEWAEGDRERYGETCIDMLNRFAPNFRGAIDQYEVLAPPDLERIFGLQG
                     GSIFQGEQGMNQMAFMRPVPDMAQYATPVGGLYLCGAGTHPGGGVTAASGHNAAQRIL
                     KDRRGVRWPWKKRATA"
BASE COUNT          301 a          568 c          595 g          294 t
ORIGIN      
        1 gctcgccttc gagatcgaga aagaacgccg ccacgcgatc gagctcgagc cggacctggc
       61 cggcgagctg gaggagctgg accaggaatt gccggagcgc tccgaggcgg gggaggcctt
      121 ctcggcgctc agctcgagcg cggccgaact cgacgacgcg atcctcgccc agaccacgat
      181 gcagctattc gccaccgacg atttcgacca tggccccggg ccggagaagt cgctggcccc
      241 ggcacccgcc gccaggaagc cgctcaagtc actggctccg aaatccgact ccgcctacgc
      301 ggtccgcgag cggctgcggg aggagcgcaa ggaactggtc gccgacgttt cccgcaggac
      361 cggcgagagc taccagcaga tcaatgcccg gatcaaccag gtgctcgggg tggcctcggt
      421 cagcaaggcg acccgggaag aactggagcg gggaaacgcc ttgctggagc gggacctgaa
      481 gatctgagtc ccttttcggc tttccggagc cattcgcgta ctagaagaaa ctttgcgttc
      541 gtcgtttgag gacgcgattt ttgggtactc tcgggtctcg ctgtttaacg atgttcgatt
      601 agggagagga agttcacaag tcatgtacgc cgcgcaaagc ctcccggggc cactcgaggt
      661 ttcggctgcg gcgcccctgc ccgaaggttc gagaaccgca ctgctggtgg tcgacatgca
      721 gaacgatttc gtcgaggaag gcgcaccccg tcaggtgcac ggtgcgctcg agatcgtgcc
      781 ggcgatcgcc agcgctgtcg agcggatgcg gattcagggc gacctgatcg tctggacctg
      841 tcgcgcctac tcggccgatg gctcgaacgt cgaggcggcc cggaaacccg ggttcaagaa
      901 gaacccgcac acggtcgccg gcacccgcgg ggccgagatc gtggacggtc tcaccccctg
      961 gccaaacgat ctcaacgtga ccacgccgtc ctactcggcc tttttccgta ccgacctgga
     1021 cctgaagctc cgcgaggccg gagtctcgaa gatcgtcggc tgtggcgtgg acctttcccg
     1081 ctgcgtaagg gcaaccctgg tcgaggcgat ctcgctcggc tacgaatgtt cggtgctcgc
     1141 gccggccgtg gccagccgtg acgaccaggc cctgaaccag aacctggccg acctcgccga
     1201 cctggacatc accgtcgaag agtgatcagg ccgtggcccg cttcttccag ggccagcgca
     1261 cgccacgccg gtccttgagg atccgctggg cggcgttgtg gccggatgcc gcagtgacgc
     1321 cgccgcccgg gtgggtgccg gcgccgcaga ggtagaggcc gccgaccggg gtggcgtact
     1381 gagccatgtc gggaactggg cgcatgaagg ccatctggtt catgccctgc tcgccctgga
     1441 agatcgagcc accctgaagg ccgaagatcc gttccaggtc cggcggggcc agcacctcgt
     1501 actggtcgat cgcgccgcgg aagttggggg cgaacctgtt cagcatgtcg atgcatgtct
     1561 cgccgtagcg ctcgcggtcg ccttcggccc actccgattc ctcggagggc ccgtactggg
     1621 tgaacatggt catgatcacc cggtcgtcgt cggtcagtga atcgtcgatc gacgacggga
     1681 actcggcctc gatgtagggg gatgcggcgg gccggccctg gagcgcgtcc tgccaggccg
     1741 cttcgagata gtcgatcg
//
LOCUS       DAOTIP010000492         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_80211_length_1757_cov_3.253231, whole genome shotgun sequence.
ACCESSION   DAOTIP010000492 DAOTIP010000000
VERSION     DAOTIP010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80211_length_1757_cov_3.253231"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>1757
                     /locus_tag="P5138_09045"
     CDS             175..>1757
                     /locus_tag="P5138_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60765.1"
                     /translation="MLVLLAGAAALLTGSLPTTGVPPADAASKADHASAAGKKSKKKA
                     CRATKKGPVARTPGYPGKCRAPGTKIAPPMPSTSLSDSGKLPDLLVDDAGTAHIVWLS
                     EGGTGPDLVHYCRMKRGSTSCDNPPATATMFPSQPSPQFNEDHAGAKIVAIGDDIAIL
                     SFRYPNVVSTPLGLSSRNLYLWISDDGGDSFSGPALVGDNEPSGDAEVFGTSDTLRIG
                     TITDTRTGGTFFQAILPGQYSGTQANLGEDGPDRAYSGSLATVNGLPMAAFSSLDGQT
                     FIRQWNGAWSPNDTANWSISEMTGREPKLAAGPGGAYLAVRDAYYKPVKIHRLNGIQP
                     AAGVTMADTADVGARDLVVDPGGTLHFAWVDRASGSEGEVRERRSPDGKKFSSTRLLA
                     RAPGNGAIGQLAVAAAGDGGGFAAYVSNGSVVGSGQVLVAPFGRQQATGLPGLGNRPG
                     GGSAPGTEVSCQRIKYGAVEMLADEGCLLSASGKAAKVSEGPIRLNGLEIIPDAGVKL
                     LLGTREKTIDSTGRVTVQLS"
BASE COUNT          305 a          595 c          599 g          258 t
ORIGIN      
        1 ccgccgccta cggccacttc gggcgcagca acgagaactt cacctgggag cgcaccgacc
       61 gcgccgacca gcttcgcgaa gtcgccgggc tctaggcgag gaaaccccat tggtcggggt
      121 tgatccggga ggcggtacgg gctagtttgg gtcatggccc ggagcctggc ccgaatgctc
      181 gtcctgctgg caggggcggc ggccctgctc accggctctt tgccgacgac gggggtgccg
      241 ccagccgacg ctgcgtcaaa ggcggatcac gcttccgccg caggcaagaa gtcaaagaag
      301 aaggcctgcc gggcgacgaa gaagggtccg gtcgcccgca cccccgggta ccccggcaag
      361 tgccgcgctc cgggaacgaa gatcgcgccc ccgatgccgt cgacaagcct gagcgattcc
      421 gggaaactcc ccgacctgct cgtcgacgac gccgggaccg cccacatcgt ctggctgagc
      481 gagggaggca cgggacccga cctcgtccac tactgccgga tgaagcgggg gtccacttcc
      541 tgcgacaacc cgccggcaac ggcaaccatg tttccgagcc agccttcgcc ccagttcaat
      601 gaggaccatg ctggagcgaa gatcgttgcg atcggtgatg acatcgccat cctcagcttt
      661 cgctatccga acgtcgtgtc cactccgctc gggctctcca gccgcaacct gtacctgtgg
      721 atttccgacg acggcggcga ctccttcagc ggcccagccc tcgtcggtga caatgaaccc
      781 tccggtgacg ccgaagtctt cggcacctcc gacaccctga ggatcggcac catcaccgac
      841 acccggaccg gagggacctt cttccaggcg atcctgcccg ggcagtattc gggcacccag
      901 gcaaaccttg gtgaagacgg tcccgaccgc gcatacagcg gttcgctcgc gacggtgaac
      961 ggcctgccaa tggccgcctt ctcgagcctc gacggccaga ccttcattcg ccagtggaac
     1021 ggagcctgga gcccgaacga cacggccaac tggagcatct ccgagatgac cggccgcgag
     1081 ccgaagctgg cggccgggcc gggaggtgcc tacctggcgg tgcgtgacgc ctactacaag
     1141 ccggtgaaga ttcatcgcct gaacgggatc cagccggcgg ccggcgtgac catggccgac
     1201 accgcagatg tcggagcgag ggacctggtc gttgacccgg gcgggaccct ccacttcgcc
     1261 tgggtggacc gggcctccgg cagcgagggc gaggtgcgcg aacggcgatc accagacgga
     1321 aagaagttct cctcgacccg gctgctcgcg agagcgcccg gtaacggggc aatcggtcag
     1381 ctggcggtcg ccgcggccgg ggacgggggc ggcttcgccg cgtacgtgtc caacggttcc
     1441 gtggtcgggt ctggccaggt gctggtcgcc ccgttcgggc gccagcaggc gaccggactc
     1501 cccggtctgg gaaaccgtcc gggtggcggc tcggccccgg gaaccgaagt ttcctgccag
     1561 aggatcaagt acggcgcggt ggagatgctg gccgatgaag gctgcctcct gagcgcatcg
     1621 ggcaaggcgg cgaaggtctc ggagggcccg atccggctga acgggctgga gatcattccg
     1681 gacgccgggg tgaagctcct gctgggcacg agggagaaga cgattgacag taccggccgg
     1741 gtcaccgtcc agctttc
//
LOCUS       DAOTIP010000493         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_80214_length_1757_cov_3.170388, whole genome shotgun sequence.
ACCESSION   DAOTIP010000493 DAOTIP010000000
VERSION     DAOTIP010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80214_length_1757_cov_3.170388"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1099)
                     /locus_tag="P5138_09050"
     CDS             complement(80..1099)
                     /locus_tag="P5138_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV60766.1"
                     /translation="MEAASFNEVDLQEQTLEVDRTDRRVELLLGALASTILLLIAGMI
                     LFVFVKALPSFTHNGLAWFGSGGSVDEQLMNIFNSPAEPENYVYHLRAWPLLYATILI
                     TGVAVLISVVFSVLSGIFIVEFAPERLRQTIEPVVKLLAAVPSVIYGLIGILVVVPFV
                     AEHLISEARKESVAYVIQLDGTSILVGVFILTIMICPIMIAIIVDALRAVPEKWVEGA
                     AALGVNRWRAMRTVSLRAIRPAIVAALVLATARALGEAIMLSMVAGSVGFAPNPLDGF
                     TFFLEPARPLASTIVDNAEGLSVAPFGQTMYAFAAILLISSAFLSFAGWAARKSFSRY
                     TLGGR"
     gene            complement(1103..>1757)
                     /locus_tag="P5138_09055"
     CDS             complement(1103..>1757)
                     /locus_tag="P5138_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV60767.1"
                     /translation="CRGCVKFKLLQGGSDVGIADVSRGRVTVGMSSRDPKSSDPGGIV
                     FNRIAKDAICLVTNKANAVSSLDQNAVQSIFGGSVRSWSQVPGAKQNGTIDLFVRTPA
                     SGTQDAFQKIFLGSTSVFSGASQKASNGLVQQAVQRDKAGIGYVSLAFTSGLVKAAYK
                     GVPCTLRNAKSGQYGGVRSFYFVTRGAPKGAVKKWIRWIRNNRAALRIAAKEWVPFK"
BASE COUNT          335 a          547 c          596 g          279 t
ORIGIN      
        1 ttcggcttcg gccgcggatc ggtcggatcg cttcccggac ccggccttgg tcccggagcg
       61 gcggtgcgaa cgtcgatgct catcggcccc ccagggtgta gcggctgaag gacttgcggg
      121 ccgcccagcc ggcgaaggag aggaaagcgc tggaaatcag caggatcgcc gcgaaggcgt
      181 acatggtctg gccgaagggc gcgaccgaga gtccttccgc gttgtcgacg atggtcgaag
      241 caagcggccg ggccggctcg aggaagaagg tgaagccgtc aagcgggttc ggggcgaagc
      301 cgaccgaacc ggcgaccatc gagagcatga tcgcctcgcc cagcgcgcgg gcggtggcga
      361 gcaccagggc ggcgacgatc gccggcctga tcgcccgcag ggagacggtc ctcatcgcgc
      421 gccagcggtt gaccccgagc gcggccgcac cctcgaccca cttctcgggc acggcccgga
      481 gcgcgtcgac gatgatcgcg atcatgatcg ggcagatcat gatagtgagg atgaaaaccc
      541 cgaccaggat gctggtgccg tcaagctgga tcacgtaggc gaccgattcc ttgcgcgcct
      601 ccgagatcag gtgctcggcg acgaagggca ccacgacgag aatgccgatc agcccgtaga
      661 tcaccgacgg gactgcggcg agcaacttga ccaccggctc gatcgtctga cgcagccgtt
      721 ccggcgcgaa ctcgacgatg aagatgccgg agaggaccga gaagacgacc gagatcagga
      781 ccgcgacccc ggtgatcagg atcgtcgcgt aaagcagtgg ccaggccctc aggtggtaga
      841 cgtagttctc cggctcggcc ggtgaattga agatgttcat caactgctcg tcgaccgacc
      901 cgcccgaacc gaaccaggcc agtccgttgt gggtgaatga cgggagcgcc ttcacgaaga
      961 cgaacaggat cattcccgcg atcaggagca ggatggtgga cgcaagcgcg ccgagcaaga
     1021 gctcgacgcg cctgtccgtc cggtcgacct ccagggtctg ttcctggagg tcgacttcgt
     1081 tgaaagaggc tgcctccatc gactacttga agggaaccca ttccttggcg gcaatacgga
     1141 gcgccgccct gttgttgcga atccagcgga tccacttctt gaccgcgccc ttcggagctc
     1201 cgcgggtcac gaagtagaag ctgcggactc cgccgtactg gccggacttc gcattgcgca
     1261 gggtgcaggg cacgcccttg taggcggcct tgaccagacc actggtgaag gccagcgaca
     1321 cgtagccgat accggccttg tcgcgctgga ctgcctgctg gaccagaccg ttggaggcct
     1381 tctgcgaagc accggagaac accgaggtgc tgcccaggaa gatcttctgg aaggcgtcct
     1441 gggttccgga agcaggggta cgaacgaaca ggtcgatcgt gccgttctgc ttggctccgg
     1501 gcacctggct ccagctgcgg accgaaccgc cgaagatcga ctggacggcg ttctggtcga
     1561 ggctggacac tgcgttggcc ttgttcgtga ccaggcagat cgcgtccttg gcgatccggt
     1621 tgaacacgat tccgcccggg tcgctcgact tgggatcgcg ggaggacatt cctaccgtga
     1681 cccgtccgcg ggaaacgtca gcgatgccga cgtccgagcc gccctggagg agcttgaact
     1741 tgacgcaacc cctgcag
//
LOCUS       DAOTIP010000494         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_80262_length_1756_cov_3.756026, whole genome shotgun sequence.
ACCESSION   DAOTIP010000494 DAOTIP010000000
VERSION     DAOTIP010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80262_length_1756_cov_3.756026"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="P5138_09060"
     CDS             <1..1031
                     /locus_tag="P5138_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60768.1"
                     /translation="SRNKIVRVISAAALITAVVYVFTPLTAAGQEFQPRGFFTNTRYL
                     LPGLLLAAVMLPLIRQLRVPEDRARKVLIFLTGVYAITVLTSPKWYPSFLAGTIFLTL
                     AIVWAPVGLSWLKANTKVPLSVIVGCVAAIAVIAAVWGRGEEVGYAEKHYTRSTLFLQ
                     EGGPQEAFDFTRDLKNKRIALAGSGEIFFGQYGFYGVDRSNHVQYIGEEGPNGTYRLI
                     DNCREFINKINEGDYDYIVTSEYTQDSPDADYWYPVRAWIDDDPAVELVVSEDNITPQ
                     PDYVYKVNGKLDPDRCAELDPKVIYSDRIEAIQAEKEREREAAIKEAEDAAAEAAAAS
                     SSDSSGSQ"
     gene            1080..1153
                     /locus_tag="P5138_09065"
     tRNA            1080..1153
                     /locus_tag="P5138_09065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1114..1116,aa:Arg,seq:tct)
BASE COUNT          340 a          562 c          589 g          265 t
ORIGIN      
        1 ggtcccgcaa caagatcgtc cgggtgatct cggcggcggc gctcataacc gccgtggtct
       61 atgtcttcac cccgctgacc gcggccgggc aggaattcca gccgcgcggc ttcttcacca
      121 acacgaggta tctgttgccg gggctgcttc tggcggcggt gatgctgccg ctgatcaggc
      181 agttgagggt gccggaggac cgggcgagga aggtcctgat cttcctgacc ggcgtttacg
      241 cgatcaccgt cctgacctcg cccaagtggt acccgagctt cctcgccggc acgatcttcc
      301 tcaccctggc gatcgtctgg gccccggtcg gcctcagctg gctgaaggcg aacacaaagg
      361 ttccgctgtc ggtgatcgtc ggttgtgtgg cggcgatcgc ggtgatcgcg gcggtctggg
      421 gtcgtggaga ggaagttggt tacgcggaga agcactacac ccggagcaca ctcttcctcc
      481 aggagggcgg tccgcaggaa gctttcgact tcacccgcga cctgaagaac aagcgaatcg
      541 cgctggccgg gtccggcgag atcttcttcg gccagtacgg cttctacggg gtggaccgtt
      601 cgaaccacgt tcagtacatc ggtgaggaag ggccgaacgg cacctaccgc ctgatcgaca
      661 actgccgcga gttcatcaac aagatcaacg agggcgacta cgactacatc gtcaccagcg
      721 agtacacaca ggactcgccg gatgcggact actggtaccc ggtccgggcc tggatcgacg
      781 atgacccggc ggtcgagctg gtggtctcgg aagacaacat tacgccccag ccggattacg
      841 tctacaaggt caacggaaag ctagaccccg atcgctgcgc cgaactcgat cccaaggtca
      901 tctacagcga ccggatcgaa gcgatccagg ctgaaaagga acgggaacgc gaagccgcga
      961 tcaaggaagc cgaagacgcg gcagcggaag ccgccgccgc gagctcgagc gattccagcg
     1021 gttcgcaata gccgcctgat cgcggccgaa gccccgaacg gctaagttca gtttcccgcg
     1081 cccccgtagc tcagctggat agagcgccgg ccttctaagc cggatgtcgc aggttcgaat
     1141 cctgccgggg gtgcctgggc cgggcggcgt ggaaggcgac caggctgacc acgaggatca
     1201 caccgccgta gatcagcgcg gtggtgtgaa gtcccagccg gtcggccgcg aaaccgccgg
     1261 ccactgccgg caggctgaag gcgatgtagc cgacgacgta ggcgagggcg tacagctcgc
     1321 cgcgggtgtc gggctcagcc atgttgacca gggccccgaa agagcccagc gccgaggcgc
     1381 cgaagccgat gccggagacg aaggtgccag ccagcgccag gggcgcgaac tcgagctcga
     1441 gtccggtcag ggaaaccgcg gttccgagag ccagccagcc ggccgccagc cggatgaccg
     1501 accgtggctc tcgttcccgc agcaggaaca cgacgaccgc gccggttccg cagagcgcgg
     1561 tcaccacgag ccccccggtc aggtagctgt cgtcatcgag aaagatcccg gcaatcgaag
     1621 gcccaagcga gaggtacagg ccggccagcg cccagccggc gatcattatc ggcaccagcg
     1681 agatgaaatc gtgccggagg tggggcggca ggcccaccct gggtcttagc gattccctgg
     1741 ccccggggtg catggt
//
LOCUS       DAOTIP010000495         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_80527_length_1752_cov_3.574543, whole genome shotgun sequence.
ACCESSION   DAOTIP010000495 DAOTIP010000000
VERSION     DAOTIP010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80527_length_1752_cov_3.574543"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="P5138_09070"
     CDS             complement(<1..890)
                     /locus_tag="P5138_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60769.1"
                     /translation="MFGKAGEYRVPLVAALLAATVATGLMLSAGVNQADAAAKYRSVS
                     SGQARAVVGTGKWSFAFRDKRKKPVLREQSGTGTSPVGRLGFQIGSNWKHATRVIKTW
                     KQGKSRLAMVKTTDPKRKLKVRVAPASNGSVRLTATIVGPSSGVEAIGMSFKAPKAER
                     YLGFGERSNAVDQRGNEVENWVGEGPYQSNEYQVVKLFVPEWGLRDRPDATYFPIPWL
                     LSTSGYGVLVENPNPSYFHLGTDRKDTWSVQLSRTVDGLARQPDDNPPPTSITLRFFA
                     GPKPADVVRRMSGALGRQPAP"
     gene            complement(925..>1752)
                     /locus_tag="P5138_09075"
     CDS             complement(925..>1752)
                     /locus_tag="P5138_09075"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60770.1"
                     /translation="YPGAFTPLKYTISGAKSGTGSGTVFTNRTEGVNCGSVCSFQVND
                     DAYVEMRAVADEGSKFVGWENAPDCPGTIPCWFNAESNRTLTAVFTDLLHKTLTVSTS
                     GAGTVTSSPAGISCGATCSHTFDTGTLVTLTAKPAPGYALSGWGGACSGKAACQVTMD
                     DEAEVSATFVKVALTLKTGKAKLNLKKGTALLPVTVNTAAKITLTGKSVKKKTASTKA
                     AKTVKMTIALTGKLKKKLRKKRKVIAKVKVTAKPVAGGSAKSKTVKVKLKLKKKKRR"
BASE COUNT          246 a          586 c          574 g          346 t
ORIGIN      
        1 ggggcgggct gccggccgag agcgccgctc attcgcctca cgacgtcggc cggcttcgga
       61 ccggcgaaga acctcagggt gatcgaagtc ggcggcggat tgtcgtcggg ctgccgggcg
      121 agaccatcga cggtgcggct caactggacg ctccaggtgt ccttgcggtc ggtgccgagg
      181 tggaagtagc tcgggttcgg gttttcgacc aggaccccgt agccggaagt ggaaagcagc
      241 caggggatcg ggaagtacgt cgcgtcgggc cggtccctga ggccccattc cggcacgaag
      301 agcttgacca cctggtactc gttggactgg taggggccct cgccgaccca gttctcgacc
      361 tcgttgccac gctggtcgac cgcgttggag cgttcgccga atccgaggta gcgctcggcc
      421 ttcggcgcct tgaaggacat gccgatcgct tcgacaccgc tggacgggcc gacgatcgtc
      481 gcggtcaggc ggaccgagcc gttgctcgcc ggggccaccc ggaccttgag cttgcgcttc
      541 gggtcggtgg tcttgaccat cgccaggcgg ctcttgccct gcttccaggt cttgatcacc
      601 cgggtcgcat gcttccagtt gctgccgatc tggaagccga gccggccgac cgggctggtg
      661 ccggtacccg actgctcgcg aagcacgggc tttttccgct tgtcgcgaaa ggcgaaggac
      721 cacttgccgg tcccgaccac ggccctggcc tgcccgctcg agaccgagcg gtacttcgcc
      781 gcggcatcag cctggttcac gcctgccgac agcatcaggc cagtcgcgac cgtcgccgcc
      841 agcagcgccg ctaccagcgg cacccggtat tcccctgcct tcccaaacac gcaggtgaat
      901 atatggaatc cggccggttc gccgctaccg gcgcttcttc ttcttgagct tcagcttgac
      961 cttgacggtc ttcgacttcg ccgaaccgcc ggccacgggc ttcgcggtca ccttgacctt
     1021 tgcgatcacc ttccgcttct ttcgcagctt cttcttcagc ttgccggtca gcgcgatcgt
     1081 catcttcacc gtcttcgccg ccttggtcga ggcggtcttc ttcttcacgc tcttgccggt
     1141 cagggtgatc tttgccgcgg tgttcacggt caccggcagc agcgcggtgc ccttcttgag
     1201 gttcagcttc gccttgccgg tcttcagggt cagtgccacc ttgacgaagg tcgccgagac
     1261 ctccgcctcg tcgtccatgg tcacctggca agcggccttg ccggagcagg cgccgcccca
     1321 gccgctcaat gcgtacccgg gggccggctt cgcggtcagg gtgacgagcg tcccggtgtc
     1381 aaaggtgtgg ctgcaggtgg cgccacaact gatcccggcc ggggacgagg tcacggtccc
     1441 ggcaccgctg gtgctcacgg tcagcgtctt gtggagcagg tcggtgaaga ctgcggtcag
     1501 ggtccggttg ctttccgcgt tgaaccagca gggaatcgtg ccggggcagt ccggggcgtt
     1561 ctcccagccc acgaacttcg agccctcgtc ggccacggcc ctcatttcga catacgcatc
     1621 gtcgttgacc tggaacgagc agacgctgcc gcagttgacg ccttcggtgc ggttcgtgaa
     1681 gaccgtgccg gaaccggttc ccgacttcgc gccggagatc gtgtacttga gtggggtgaa
     1741 agccccgggg ta
//
LOCUS       DAOTIP010000496         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81177_length_1742_cov_3.728512, whole genome shotgun sequence.
ACCESSION   DAOTIP010000496 DAOTIP010000000
VERSION     DAOTIP010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81177_length_1742_cov_3.728512"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="P5138_09080"
     CDS             complement(<1..196)
                     /locus_tag="P5138_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60771.1"
                     /translation="MRFLLAALAATLAAAAFVPVASANNTRVTIADFQWSNKTPHVDL
                     GETVRWTWTGPDTQHSITGQP"
     gene            complement(217..1242)
                     /locus_tag="P5138_09085"
     CDS             complement(217..1242)
                     /locus_tag="P5138_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRV60772.1"
                     /translation="MDRRNFLGVAGGAFVCTIAGKQYSINRHTDLSELAGKIPVPPKV
                     AAAKRTNTITAADISGNRKEFWIQAEPVIWNPVPKKRDQMMGSSIKKVVGRTKVPAYA
                     YRLYDTDFSKPLGPATIPGPLLDVTVGDTLVVHFRNACDIPVTMHPHGAFYSNEMDGA
                     YKGYWTDPGGFVQTGRTFTYVWECPEGTQGSWHYHDHGPLDSTSVFKGLFGPLIIRDP
                     DEPLPDREYFLAFHAFTPGLFPLKSPLHSINGRAYTGNTPTLRAKVGDDVAMHIYGMD
                     DDFHTFHIHGHRWTEPGGTIVDNKTFGPADSFRVRWIEDNPGRWLYHCHVFTHLAMGM
                     SGWYIVE"
     gene            complement(1284..>1742)
                     /locus_tag="P5138_09090"
     CDS             complement(1284..>1742)
                     /locus_tag="P5138_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRV60773.1"
                     /translation="TDATASGLTAKQKRAKKKALKKCYKIRNKAKRKRCVKNVNRKYK
                     KLSQSQVKPKVAVTVGVNDDYFSPSSSTMKKGQAIKWVWNNNNRNAHNVTLVSGPAGL
                     TAKDKYNLTTPNSPAVGYTFGPKVLNQTGTYNFVCSLHSTVMVFSVNVTN"
BASE COUNT          310 a          522 c          553 g          357 t
ORIGIN      
        1 gaggctggcc ggtgatcgag tgctgggtgt caggacccgt ccaggtccag cgcaccgtct
       61 cgccgagatc gacatgaggg gtcttgttcg accactggaa gtcggcgatc gtgacccgcg
      121 tgttgttggc cgaagccacc gggacgaatg cggcagccgc cagcgtcgcc gcaagcgccg
      181 ccagaagaaa ccgcatgacc ctgctccggt cggatgtcac tcgacgatgt accaaccgct
      241 cattcccatc gccaggtgcg tgaagacgtg acagtggtag agccaccggc cggggttgtc
      301 ctcaatccac ctgacccgga acgaatcggc cggaccgaaa gtcttgttgt cgacgatcgt
      361 cccgcccggc tcagtccacc ggtgtccgtg aatgtggaag gtgtggaaat cgtcgtccat
      421 gccgtagata tgcatggcaa cgtcatctcc gaccttggcc cgcagggtcg gcgtattgcc
      481 cgtgtacgca cgcccgttga tcgaatggag cggcgacttg agcgggaaga ggcccggcgt
      541 gaatgcgtgg aaagcgagga agtactcgcg atccggaagc ggctcgtcgg ggtcacggat
      601 gatcagcgga ccgaacaggc ccttgaagac cgaggtcgag tcgagcggac cgtggtcatg
      661 gtaatgccag ctgccctggg tcccttcggg gcactcccag acataggtga aagtacgacc
      721 ggtctggacg aatccccccg gatcggtcca gtagcccttg taggcaccgt ccatctcgtt
      781 cgagtagaag gcgccatgcg gatgcatggt caccgggatg tcgcaggcat tccggaaatg
      841 gaccaccagc gtgtctccga ccgtgacgtc cagcagcggc ccggggatag tggccggccc
      901 gagcggcttg ctgaagtcag tgtcgtagag acggtaggcg taggccggaa ccttggtccg
      961 gcccacgacc ttcttgatcg acgagcccat catctggtcc cgcttcttcg ggaccggatt
     1021 ccagatcacc ggttcagcct ggatccagaa ctccttgcgg ttgccggaga tgtcggccgc
     1081 ggtgatcgtg ttggtccgct tcgcggccgc caccttcggg ggcaccggga tcttgcccgc
     1141 caactccgaa agatcggtgt ggcggttgat cgagtactgc ttgccggcga tggtgcagac
     1201 gaaagcacca ccggcaacgc cgaggaagtt cctgcggtcc attagatccg gtcctctctt
     1261 ggctctgcca gacgcagttg cgcctagttc gtcacgttga ccgagaaaac catcacggtc
     1321 gagtgaagcg agcagacgaa gttgtaagta ccggtctggt tcaggacctt gggtccgaac
     1381 gtgtaaccga ccgccgggga attcggcgtg gtcaggttgt acttgtcctt cgccgtgagg
     1441 cctgccggac ccgaaaccag ggtcacgtta tgggcgttcc ggttgttgtt gttccagacc
     1501 cacttgatgg cctggccctt cttcatcgtc gatgacgacg gcgagaagta gtcgtcgttg
     1561 actccaaccg tgacagccac cttcggcttg acctggctct gggagagctt cttgtacttc
     1621 cggttgacgt tcttcacgca ccgcttgcgc ttcgccttgt tgcgaatctt gtagcacttc
     1681 ttgagcgcct tcttcttggc cctcttctgc ttcgcggtaa ggcccgaagc ggtcgcatcc
     1741 gt
//
LOCUS       DAOTIP010000497         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81241_length_1741_cov_3.693357, whole genome shotgun sequence.
ACCESSION   DAOTIP010000497 DAOTIP010000000
VERSION     DAOTIP010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81241_length_1741_cov_3.693357"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="P5138_09095"
     CDS             <1..469
                     /locus_tag="P5138_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HRV60774.1"
                     /translation="QLVEGGTTLLLTTQYLEEADQLANRVGIIDAGKIAAEGTPAALK
                     AEIGRPSVEVLPVDPARRKEAEDILLRFGSAVPAAGEALSVRLNEGIEELAAIVRSFD
                     SAGIELASLGLNEPTLDDVFLAKTGRHLEGAGEAESAEMPLPASDPDEQAGPA"
     gene            466..1302
                     /locus_tag="P5138_09100"
     CDS             466..1302
                     /locus_tag="P5138_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60775.1"
                     /translation="MSGAVATAGVGVADPGAPDPGPSPLPIQISAMASRSIKRLIRQP
                     ALVIPSVIFPLFLLAVNAGGLNAATHIPGFPTDSYLTFAIGFTFIQAAIFSTMGAGQS
                     LAQDIENGFFSRLVLTPMRPTALMAGQLAGLVLLGQFQALLYLSVGLAAGANFEAGVA
                     GYFVLAALVSIISIGFGGIGLIMAIRTGSGEAVQGMFPLMFVLLFFSSMTLPRNLIEQ
                     DWYRFLATINPVSYLIEAIRSLFIYGWDAEALALGFGIAAIVAVLSITFAARSLKGRM
                     LR"
     gene            1299..>1741
                     /locus_tag="P5138_09105"
     CDS             1299..>1741
                     /locus_tag="P5138_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60776.1"
                     /translation="MTPDRTYFSAAWAVAWRYLHNLYTKPAVFVPGLIFPLFFLAAFA
                     GGLSAVGDTPRFDYYNFTAFQFCFILIQASALAGVFVGFSIAGDFEQGMGRRMMLATS
                     RRSSILLGYALGAALRLIVVWVIITGVSLLAGMEVRATGYDLAGM"
BASE COUNT          256 a          600 c          548 g          337 t
ORIGIN      
        1 gcaactcgtc gagggcggca ccaccctgtt gttgaccacc cagtacctcg aggaagccga
       61 ccagctcgcg aaccgggtcg ggatcattga cgccggcaag attgccgccg agggaactcc
      121 cgcggccctc aaggcggaga tcggccggcc cagcgtcgag gttctgccgg tcgatccggc
      181 ccgcaggaaa gaggccgaag acatactcct ccgtttcggc agcgcggtcc cggcggccgg
      241 cgaggcgctc agcgtccggc tcaacgaagg catcgaggaa ctcgcggcaa tcgtgcggtc
      301 ctttgattcg gccggaatcg aactggccag cctcggcctg aacgaaccca cgctcgatga
      361 cgttttcctc gccaagaccg gtcggcacct cgagggagcc ggggaggcag aaagcgccga
      421 aatgccattg ccggcttccg atccggacga acaggcggga ccggcatgag cggggccgta
      481 gcgaccgccg gagtcggggt cgcagacccg ggcgcgccgg accccggccc gtcgcccttg
      541 ccgatccaga tctcggccat ggcgagccgc tcgatcaagc ggctgatccg ccagccggcg
      601 ctggtcatac cgagcgtaat cttcccgctc ttcctgctcg ccgtgaacgc gggtggcctc
      661 aacgcggcga cccacatccc cgggtttccg accgactcgt acctgacctt cgcgatcggc
      721 ttcaccttca tccaggccgc gatcttctcg accatgggcg ccgggcagag cctggcccag
      781 gacatcgaga acggattttt cagccggctg gtgctcactc cgatgcgtcc caccgccctg
      841 atggccggtc agctggccgg actggtgctg ctgggccagt tccaggcgct gctctacctt
      901 tcggtcggtc tcgcggccgg ggccaacttc gaggccgggg tggccggcta cttcgtgctg
      961 gcggcgctgg tttcgatcat ctcgatcggc ttcggcggaa tcggcctgat catggcgatc
     1021 cgtaccggtt caggggaggc ggtccagggc atgtttccgc tgatgttcgt gctgctcttc
     1081 ttcagttcga tgaccctgcc ccgaaacctg atcgagcagg actggtaccg gtttctggcg
     1141 accatcaatc cggtcagcta cctgatcgag gcgatccgca gcctgttcat ctacggctgg
     1201 gacgctgaag ccctcgccct cggcttcggg atcgccgcca tcgtcgccgt tctttcgatc
     1261 accttcgccg cccgttcact gaagggaagg atgctcaggt gacgcccgac cgcacctact
     1321 tctccgccgc ctgggctgtc gcctggcgat acctccacaa cctgtacacc aagccggccg
     1381 tcttcgtgcc cggcctgatc tttccgctgt tcttcctcgc cgccttcgcc ggaggcctct
     1441 cggcggttgg agacacgccc cgcttcgact actacaactt caccgccttc cagttctgtt
     1501 tcatcctgat ccaggcctcg gccctggccg gggtcttcgt cgggttttcg attgccggcg
     1561 acttcgagca ggggatgggg aggcggatga tgctggctac ctcgaggcgt tcgtcgatcc
     1621 tgctcggtta cgcgctcggc gcagccctgc ggctgatcgt tgtctgggtg atcatcaccg
     1681 gcgtttccct gcttgccggc atggaggtgc gggccaccgg ttacgacctg gccggcatgt
     1741 a
//
LOCUS       DAOTIP010000498         1740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81316_length_1740_cov_2.693175, whole genome shotgun sequence.
ACCESSION   DAOTIP010000498 DAOTIP010000000
VERSION     DAOTIP010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81316_length_1740_cov_2.693175"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1562)
                     /locus_tag="P5138_09110"
     CDS             complement(276..1562)
                     /locus_tag="P5138_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60777.1"
                     /translation="MASSARRPINAPPGSPQGTGVPWLGLSILLVLLLILAAFVAYFF
                     FDVGKDEGGDSSSGVTELETGATGGQGAIVGHSNGPLGFPSAATRNTTRISGSDPVDI
                     SMAAALAAYPTSGPGSPPAAVTIIDANDWQSGVAAASLSALPIGSPILLAPSGTLSAT
                     GMDTLAQLDPLGSPDTGENKVFTVGKVAPPSGYDVERLDGNSPAEIAAAVADERAALT
                     DAPPNAFVVVSSEAPDYATPAATWAARSGDVVLYTGKDEVPKATLDVIKDKANKDVPI
                     FVLGPSDVVSSKALKQLDKAGSTVERVGGDDPESAAVELVRFSSGLFGWSLNEPGHGY
                     VLARTDRPMDAIAVTALSTGGTWAALLLTDSSEKLPQVVEDYLLDVKPGYETDPTKAI
                     FSHVWIVGDDSIISVDEQARVDDAVELAPVDTVAEG"
BASE COUNT          326 a          539 c          566 g          309 t
ORIGIN      
        1 gagggcggct caaccactgg agttgcttgc atgaaccctg gagggcaacg acctgacccg
       61 ggaaagttca atcgggtcga ttcgtctgac gtgacctgtc agtagtgact aggtgtgcgg
      121 tcggattcgt ttctggccgc acacccaagt cccaacattg agagacgtcc gcgtagccga
      181 ctacgcaggc gcctttgtaa ctgagaatcc ccgatctcag ggtagcccaa cattgtcccc
      241 gaaaagtgga cgaaagtagt cccaacgttt cgggttcatc cctcagccac agtgtcgacg
      301 ggggcaagct cgaccgcgtc atcgacacgc gcctgttcgt ccactgagat aatcgagtcg
      361 tcgcccacga tccagacgtg ggagaaaatc gctttggtcg ggtcggtctc atagccgggc
      421 ttgacatcaa ggagatagtc ctcgaccact tgcggcagct tctcgctgga atcggtcagg
      481 agcagagccg cccaggtacc acctgtagag agtgcggtca cagcgatcgc atccatcggg
      541 cgatctgtcc gggccaggac atagccgtgt ccgggctcgt tcagactcca gccgaagagg
      601 ccgctcgaga agcggaccag ctcgaccgcg gcggattcag ggtcatcgcc gccgacccgc
      661 tcgaccgttg agccggcctt gtcgagttgt ttcagggcct tgcttgagac cacatccgac
      721 ggtccgagca cgaagatcgg gacatccttg ttggccttgt ccttgatcac gtcgagggtg
      781 gccttcggca cctcatcctt tccggtgtag aggacgacgt ctccggatcg ggcggcccag
      841 gtcgcggccg gagtggcgta atcgggagcc tcggaggaga cgaccacgaa ggcgttaggt
      901 ggtgcatcgg tcagggcggc gcgctcgtct gccactgcag cggcaatctc ggccgggctg
      961 tttccgtcca gccgttcaac gtcatagccg gaaggcgggg cgacctttcc taccgtgaaa
     1021 accttgttct cgccggtatc gggcgaaccg agcggatcga gctgggccag cgtgtccatg
     1081 ccggtcgctg aaagcgtgcc cgacggggcg agcaggatcg gcgagccaat cggcagcgca
     1141 ctgagcgaag ctgccgcgac tcccgactgc cagtcgttcg cgtcgatgat cgtgaccgcc
     1201 gccggtggcg aaccgggtcc tgaagtgggg taggcggcca gcgcggcggc catcgaaatg
     1261 tcaaccggat cgctgccgct gatcctggtc gtgttgcggg tggccgccga ggggaaaccg
     1321 agcgggccgt tcgagtgacc gacgatcgcg ccctggcccc cggttgcgcc ggtttccagc
     1381 tcggtcacgc ctgaggagga atcgccccct tcgtccttgc ccacgtcaaa gaagaaatag
     1441 gcgacgaagg cggccagaat cagcagcagg acgagcagga tcgagaggcc gagccagggc
     1501 accccggtgc cctgcggact ccctggcggt gcgttgatcg gccgtcttgc ggagcttgcc
     1561 acggcgtgaa ccgtatccaa acggtgtcgt tcaccggaaa tcgtaagaat ggcggcgatg
     1621 ccatacccgg ttgggacaac caccaatgac gccggccacc tcgagattgg aggctgcgac
     1681 gtaatcgaac tcgcccgcga gttcggcacc ccgtcctacc tcttcgccac ggccgaaatg
//
LOCUS       DAOTIP010000499         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81436_length_1738_cov_3.238265, whole genome shotgun sequence.
ACCESSION   DAOTIP010000499 DAOTIP010000000
VERSION     DAOTIP010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81436_length_1738_cov_3.238265"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="P5138_09115"
     CDS             <1..955
                     /locus_tag="P5138_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRV60778.1"
                     /translation="GLVVLAVLIGLLIAWAATRSHTVDVPNMVGKSSDTAVSRLNEKG
                     FKVEVERQQNPSVPSNQVFSQNPRGEADQDCNFLGFSCSNPTVTLKVSGGPGQTEVPD
                     VTGLSQADAEQQLSEAGFTTSVETKASSDVDSGLVIETDPPAGETVKRGSQVTITVSS
                     GISKVKVPPVVGQTLNAAKQQLAAVGLDFDSTEKPSDRPQGEVIDQSPTAGTRVDPGT
                     AVSLTVSSGPEDTRVSIPSVVGLTQSAAESQLSSAGLVASVQTETTTIQPQDGKVIDQ
                     SPISGTKVTEGSTVVITVGKYEAGSSGTGTGTDGSGGIGAN"
     gene            1027..>1738
                     /locus_tag="P5138_09120"
     CDS             1027..>1738
                     /locus_tag="P5138_09120"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HRV60779.1"
                     /translation="MRVAVLSGGRSSEHDISLLSAEAVAEGLDTAGHDVIRITIGKSG
                     AWIHDGHPVEVRPGEGLAVAGDLLSLDAVFPVLHGPFGEDGVTQGALDTAGLPYVGSD
                     VLSSAVCMDKLTLKRLCGFHGIPQVGFVQAFTPGWEEEALALEFPLWVKPSRLGSSVG
                     ISKVTDPADLAAAVDEAAKFDPRVIIEANSGGREIECSVLGNEDPEASLPGEILTDDE
                     WYDYEAKYTEGRTTIAAPA"
BASE COUNT          314 a          586 c          560 g          278 t
ORIGIN      
        1 cgggctggtc gtgctggccg tcctgatcgg attgctgatc gcctgggccg cgacccggag
       61 ccacacggtc gacgtgccca acatggtcgg aaagagcagc gacacggccg tttcgagact
      121 gaacgagaag ggcttcaagg tcgaggtcga acggcaacag aatccgtccg tgcccagtaa
      181 ccaggtcttc agccagaacc caagggggga agcggaccag gactgcaact tccttggctt
      241 ctcctgctcg aacccgaccg tgaccctgaa ggtcagcggc ggacccggcc agaccgaagt
      301 ccccgacgtg accggcctca gccaggccga tgccgagcag cagctttccg aggccggttt
      361 cacgaccagc gtcgagacca aggcgtcttc ggacgtcgat tcgggactgg tgatcgaaac
      421 ggatccgccc gccggtgaaa cggtcaagcg gggctcgcag gtgaccatca cggtctccag
      481 cgggatctcc aaggtgaaag ttccaccggt cgtcggccag accctgaacg cggccaagca
      541 gcagctcgcg gccgtaggcc tcgacttcga ttcgaccgag aaacccagcg atcgcccgca
      601 aggcgaggtg atcgaccagt cgcccaccgc gggcaccagg gtcgacccgg gcacggcggt
      661 cagccttacc gtttcctctg gccctgaaga cacccgggtt tcgatcccgt cggtggtggg
      721 actgacccag agcgccgcgg agtcccagct ttcctctgcc ggccttgtcg ccagcgtcca
      781 gaccgagacc acgacgatcc agccgcagga cggcaaggtc atcgaccaaa gcccgatctc
      841 cggcaccaag gtgaccgaag gttcgaccgt ggtgatcacc gtcggcaaat acgaagcggg
      901 ttcttcggga accggaaccg gaacggacgg cagcggcggc atcggcgcca actgattcac
      961 gctcttcgcc tgttcgggcg ggccaacgcc cctcccggcg ggccgaccaa atatcgtttc
     1021 ccttcgatga gagttgcagt cctcagcggt ggccgttcca gcgaacatga catttcgctg
     1081 ctctccgccg aggcggtagc ggagggactc gacaccgccg gccacgacgt catccgcatc
     1141 acgatcggga agtccggagc ctggatccac gacggccacc cggtggaggt ccgtcccggc
     1201 gaaggactgg ccgtggccgg ggatctcctt tcgctcgacg cggtcttccc ggtcctgcat
     1261 ggcccgttcg gcgaggacgg tgtcacccag ggcgccctgg acacggccgg ccttccctat
     1321 gtcggttcgg acgtcctctc ctccgcggtc tgcatggaca agctgaccct gaagcgactc
     1381 tgcggctttc acgggatccc ccaggtcggt ttcgtccagg ccttcactcc cggctgggag
     1441 gaggaggccc tcgcgctcga gtttcccctc tgggtcaagc cttcccgtct cggctcgagc
     1501 gtcgggatca gcaaggtcac cgatccggcc gaccttgcgg cggcggtcga cgaagcggcg
     1561 aagttcgatc cacgggtgat catcgaagcg aattcgggcg gtcgcgaaat cgagtgctcg
     1621 gtgcttggca acgaggatcc ggaggcatcg ctgccgggcg agatcctcac cgatgacgag
     1681 tggtacgact acgaggccaa gtacaccgag ggtcgcacca cgatcgccgc cccggccg
//
LOCUS       DAOTIP010000500         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81455_length_1738_cov_2.586453, whole genome shotgun sequence.
ACCESSION   DAOTIP010000500 DAOTIP010000000
VERSION     DAOTIP010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81455_length_1738_cov_2.586453"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1738)
                     /locus_tag="P5138_09125"
     CDS             complement(<1..>1738)
                     /locus_tag="P5138_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRV60780.1"
                     /translation="DAVLISGEGIFTGKAPSTPEDQSIGVIEAVRQVIEKSGIEVSEV
                     GHFAHGMTVATNALLESKGAETAFVATRGFTDLIEIGRQDRASLYRLEAAHPPPLVPP
                     DRRVGADERCGPEGVIRDLEPAEAARVADQVAEMGVESVAVCLLHSYRFPDHEKEIGE
                     AIRQRLGSGVHVSLSHDVVGTFREFERASTTEIDAALSPLLAGYLERLARTCADEGLP
                     EPEIMQSSGGVAGLDEVSAHAAVAILSGPAGGAAAAAMVSRHSGEENLLCFDMGGTSC
                     DVCAVEDGAIHEAASKKIAGRPIALPMVDIDTVGAGGGSIAWRDSGGALRVGPESAGA
                     RPGPACYGLGGTRPTVTDANLMLGRLAVGDELAGGIVLDEELAVKAIGELADQLGVSG
                     EECAEGIIRVANSEMVRALRVVTVQRGLDPRRFTLLAYGGAGPLHAAGVAEELGITSI
                     LIPVAGGVFSALGLAAADRRRDEVQTVLAAEEELDHEKLAGLIGEADEAGWDLRYRGQ
                     SFELTVTDHSADPARLRELFESEHEDRYGYSDPSAPIELVTVRRRFIEEGPGFSAGPP
                     PEEELEGPARVDL"
BASE COUNT          268 a          618 c          580 g          272 t
ORIGIN      
        1 cgaggtcgac ccgggcgggg ccctcaagct cctcctcggg gggcggcccg gccgagaagc
       61 ccggcccctc ctcgatgaag cggcgacgga ccgtcaccag ttcgatcggt gccgaagggt
      121 ccgaataccc gtagcggtct tcgtgctcgg attcgaaaag ttcgcgcagc ctggcggggt
      181 cggcggaatg gtcggtgacc gtgagttcga aggactggcc gcggtagcgg aggtcccagc
      241 cggcctcgtc ggcctctccg atcaggcctg ccagcttctc gtggtcgagt tcctcctcgg
      301 ccgcgagcac ggtctggacc tcgtctcgtc tccggtccgc ggcggccagg ccgagcgcgc
      361 tgaagacccc gccggcgacc ggaatcagga tcgaggtgat gccgagttcc tcggcgacgc
      421 ccgccgcgtg gaggggtccg gccccgccgt aggcgagcag ggtgaatcgc cgcggatcca
      481 ggccgcgctg gacggtgacc acccggaggg cgcggaccat ctccgagttg gccacccgga
      541 tgatcccctc ggcgcactct tcgccggaga cgcccagctg atcggccagt tccccgatcg
      601 ccttcacggc aagctcttcg tcgaggacga tgccgccggc cagctcgtcg ccgaccgcca
      661 gcctgcccag catgagattc gcgtcggtca ccgtgggccg ggtcccgccg agcccgtaac
      721 aggcgggtcc cggcctggcc ccggccgact ccggccctac ccggagagcg cccccgctgt
      781 cgcgccaggc gattgaaccg cctccggcac ccaccgtgtc tatgtccacc atcggcagtg
      841 cgatcggccg gcccgcaatc ttcttcgagg cggcttcgtg gatggctccg tcttcgaccg
      901 cacacacatc gcaggaggtg ccacccatgt cgaaacagag cagattttct tccccggaat
      961 gacgggagac catcgctgcc gctgccgccc cgccggccgg accggaaagg atcgccaccg
     1021 cggcatgggc ggaaacctcg tccaggcccg ccacgcctcc gctcgactgc atgatctcgg
     1081 gctcgggcag cccctcgtcg gcacaggtcc gggcaagccg ttccagatag ccggccagca
     1141 gcggagagag ggcagcgtcg atttcggtgg tagacgcccg ttcgaattcg cggaaggtgc
     1201 cgaccacgtc gtgggaaagg gagacgtgga cgccggagcc gagacgttgc cggatggctt
     1261 ccccgatctc cttctcgtgg tcgggaaaac ggtacgagtg gagcaggcag accgcgaccg
     1321 attcgactcc catctccgca acctggtcgg ccacccgtgc tgcctcggcg ggttcgagat
     1381 ccctgatcac cccttccggg ccgcaacgct cgtcggctcc gaccctcctg tccggaggaa
     1441 cgaggggcgg gggatgggcg gcctcgagcc ggtagaggga cgcccggtcc tggcggccga
     1501 tctcgatcag gtcggtgaac ccccgggtcg ccacgaacgc ggtctcggct cccttcgatt
     1561 cgagcagcgc attggtggcg accgtcatgc cgtgggcgaa gtgacccacc tcggagacct
     1621 cgatgcccga cttttcaatc acctggcgga ccgcttcaat caccccgatc gactgatcct
     1681 cgggagttga aggcgccttg ccggtgaaga tgccttcacc cgagatcagg actgcatc
//
LOCUS       DAOTIP010000501         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81645_length_1735_cov_3.847619, whole genome shotgun sequence.
ACCESSION   DAOTIP010000501 DAOTIP010000000
VERSION     DAOTIP010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81645_length_1735_cov_3.847619"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="P5138_09130"
     CDS             <1..560
                     /locus_tag="P5138_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV60781.1"
                     /translation="DFRPDLIVLDVMMPGLDGFQVAKRLAERSNDVPILFLTARDTTD
                     DKIRGLTIGGDDYMTKPFSVEELIARIKVILRRSGRSREESPRLTYLDLELDDDTREV
                     FRDGNPIELTDTEFRLLRYLMSNPRRVLSRAQILDHVWEYDFAGDARVLETYVSYLRR
                     KLDKYGPSLIVTVRGVGYALREPKG"
     gene            563..>1735
                     /locus_tag="P5138_09135"
     CDS             563..>1735
                     /locus_tag="P5138_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV60782.1"
                     /translation="MSLRLRLLIGILVLAAAGLLTLDFVSYRALENHLSDRVDEQVQS
                     AAMSLAPELREKAGIVDIGPVFPSGSGFDHPPQGGSSGRLGPGGGLPNELPPGTFAQL
                     KSSSGEVLATAVFGYGTTSVTRPDLSGDIPVSEFGQPLKTVTVGARGGSGTQFRVSAV
                     ADQDGRVTYVAVPTSDMKETLDQLLLIEAVVTAAILIALAGLAWWMIGIGLRPLERMS
                     ETAGEIAAGDLSRRVEDEDPKTETGRLGISLNTMLHQIEDSVRKREQSEEKMRQFLAD
                     ASHELRTPLASIRGYAELYRMGAIPEGPEADRAMDRIEKESARMGDLVDGLLTLARLG
                     ELPEPVRRPVDLAVVASECRDDAKAAAPDREFSLDTAEDVTVQGDEDQIRQLLANLL"
BASE COUNT          296 a          596 c          578 g          265 t
ORIGIN      
        1 ccgacttccg gcccgacctg atcgtgctgg acgtgatgat gcccggcctc gacggcttcc
       61 aggtcgccaa gcgcctggcc gaacgcagca atgacgtccc gatcctcttc ctgaccgccc
      121 gcgacacgac cgacgacaag atccgcggcc tgaccatcgg cggcgacgat tacatgacca
      181 agccgttctc cgtagaagag ctgatcgcgc ggatcaaggt gatcctgcgc cgctccggcc
      241 gcagccgcga ggagtcgccc cgcctcacct acctcgacct cgaactcgac gacgacaccc
      301 gcgaggtgtt ccgtgacggc aacccgatcg agttgaccga caccgaattc cgtctgctcc
      361 gctacctgat gagcaacccg cgccgggtcc tttcccgggc gcaaatcctc gatcatgtct
      421 gggaatacga cttcgccggc gacgcccgcg tactcgagac ctacgtcagc tacctgcggc
      481 gcaagctcga caagtacggc ccgagcctga tcgtcacggt gcgcggagtc ggctacgcat
      541 tgcgcgaacc caagggctga ccatgtcgct gcggctgcgg ctcctgatcg ggatcctggt
      601 gctcgctgcc gcgggactcc tgaccttgga cttcgtctcc taccgggccc tggaaaacca
      661 cctctccgac cgggtcgacg agcaggtcca gagcgccgcg atgtcgctgg cgccggaact
      721 tcgcgagaaa gccgggatcg tagacatcgg gccggtcttc ccgagcggat cgggattcga
      781 ccatccgccg cagggcgggt cctccggccg gctcgggccg ggtggcggac tgccgaacga
      841 gctcccgccc ggaaccttcg cccagttgaa gtcctccagc ggggaggttc tggcgaccgc
      901 cgttttcggt tacggcacca cgagcgtgac cagacccgac ctgagcgggg acatccccgt
      961 gagcgaattc gggcagccgc tcaagacggt gaccgtcgga gcccggggcg gttccggcac
     1021 gcagttccgg gtgagcgccg tggccgacca ggacgggcgg gtgacctatg tggccgtgcc
     1081 gaccagcgac atgaaggaga cgctcgacca gctgcttctg atcgaggcgg tcgtcacggc
     1141 ggcgatcctg atcgccctgg cgggcctggc ctggtggatg atcggcatcg gcctccggcc
     1201 cctcgaacgg atgagcgaga ccgcgggcga gatcgcggcc ggggatcttt cccggcgggt
     1261 cgaggacgag gacccgaaga ccgagacggg ccggctcggc atctccctca acacgatgct
     1321 tcatcagatc gaggactcgg tccgcaagcg cgagcagagc gaggagaaga tgcgccaatt
     1381 cctcgccgac gcttcgcatg agttgcgaac accgctggcg tcgatccgcg gctacgcgga
     1441 gctctaccgg atgggggcga tccccgaagg ccccgaggcc gaccgggcga tggaccggat
     1501 cgagaaggag tcggcccgga tgggcgacct ggtcgacggg ctgctgaccc tggctaggct
     1561 cggcgagttg cccgaacccg tacgcaggcc ggtggatctc gccgtggtcg cctccgaatg
     1621 ccgcgatgac gcgaaggccg cggcccctga ccgagagttc agcctggaca ctgccgagga
     1681 cgtgacggtc cagggcgacg aggaccagat ccgtcagttg ctcgccaatc tcctt
//
LOCUS       DAOTIP010000502         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_81939_length_1731_cov_2.979714, whole genome shotgun sequence.
ACCESSION   DAOTIP010000502 DAOTIP010000000
VERSION     DAOTIP010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81939_length_1731_cov_2.979714"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="P5138_09140"
     CDS             complement(<1..942)
                     /locus_tag="P5138_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HRV60783.1"
                     /translation="MGRPPSRQERQKRLKTRALPLVGVALISFIVGMVKGCPGSPNRD
                     AAEQYVEYWAEGNYVAMYDMLSTKSREGITQERFIARYGEASAEATIESIEQSDAEGD
                     ETNAEIPMEVDTLAFGTIDQSLKVTFGSDGIAWQNSLLFPGLELNEELTRVTKLPGRA
                     KILAEDGQVMAEGPVDAREHPLGDSMIDVTGVTGPPDQEEGPDLTSLALGYEPGEDTG
                     ISGLELAYNARLTGTPGGTLFAKPIEGGTDGQGRELGSGTPAPAKPLKTTIDPDLQES
                     AVANLGGVSGGVTVLDAKKGFIKALAGSAYSVLQPPGS"
     gene            1024..>1731
                     /locus_tag="P5138_09145"
     CDS             1024..>1731
                     /locus_tag="P5138_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRV60784.1"
                     /translation="MTALDWGIAAFTFLLALWGFRQGLIVGALGLAGLAVGAILGSRL
                     APVFLDHGSNSPYAPLAALVGAIFVGSITLALSVTLGERVRDSTVSHPFWEVIDGIGG
                     AVLIAAVGLGIVWVLAAVAVYYPSSDGLRRDVQKSLLVGAVNDVMPPSGPLMQALHRI
                     DPFPRIAASTGEIAKPDQGTGSKAAVQKAAESVVKVSGTCNGFGVMGSGWAVGPDTFV
                     TNAHVVAGQDDTTIQTND"
BASE COUNT          248 a          563 c          574 g          346 t
ORIGIN      
        1 cgatccgggc ggctgcagca ccgaataggc ggaaccggcc agagccttga tgaagccctt
       61 cttcgcgtcg agcaccgtca cgccgccgga gaccccgccg aggtttgcca ccgccgactc
      121 ctgcagatcc gggtcgatcg tggtcttcag cggtttggcc ggggccgggg tgccgctgcc
      181 gagctcgcgc ccctggccat cggttccacc ctcgatcggc ttggcgaaca gggtgccccc
      241 gggggtgccg gtgagccgcg cgttgtaagc gagttcaagc ccgctgatgc cggtgtcctc
      301 gccgggttcg taaccgaggg cgaggctggt caaatccggg ccctcttcct ggtcgggcgg
      361 tccggtgacc ccggtcacgt cgatcatcga gtctccgagc ggatgctcgc gggcgtcgac
      421 cggaccctcg gccatcacct gcccgtcttc ggcgaggatc ttcgcccggc cgggcagctt
      481 ggtgaccctg gtcagctctt cgttcagttc gagcccgggg aagagcaggc tgttctgcca
      541 ggcgattccg tccgaaccga aggtgacctt gagcgactgg tcgattgtcc cgaaagcgag
      601 agtgtccact tccatcggga tttcggcgtt ggtttcatcg ccttcggcat cgctttgttc
      661 gatgctctcg atcgtcgcct cggccgaagc ttccccgtac ctggcgatga atcgctcctg
      721 ggtgatccct tcccgggact tggtgctgag catgtcgtac atcgcgacgt aattgccttc
      781 ggcccagtac tcgacgtact gctcggcggc atcacggttg gggctgccgg ggcatccctt
      841 gaccattccg acgatgaaag agatcagcgc gactccgacc agaggcaaag ccctcgtctt
      901 gagtcgtttt tgccgttcct ggcgtgaagg aggacgcccc atctgggctg aaaaataccg
      961 gggtcgccgg tggttagatg ttgcggctga ccatcggtgc cgcccggggg ctaaattggc
     1021 cgggtgacag cgctggactg gggcatcgcc gccttcacct tcctgcttgc cctctggggt
     1081 ttccgtcaag ggttgatcgt cggtgcgctg ggactggccg gactcgcggt cggggccatc
     1141 ctgggatccc ggctggcccc ggtctttctc gaccatggct cgaactcgcc ctacgcgcca
     1201 ctggccgcgc tggtcggggc gatcttcgtc ggttccataa ccctggccct ctcggtcacg
     1261 ctcggcgagc gggtacggga ttccacggtc agccatccat tctgggaagt gatcgacggg
     1321 attggcgggg cggttctgat cgctgccgtc gggctgggca tcgtctgggt cctggctgcg
     1381 gtcgccgtct actacccgag ttcggacggc ctgcgtcggg acgtccagaa gtcactcctg
     1441 gtcggtgccg tcaacgacgt gatgcccccg tcggggcctt tgatgcaggc cctccaccgg
     1501 atcgatccct tcccccggat cgccgcttcg accggtgaga tcgccaaacc ggaccagggc
     1561 accggcagca aggcagcggt gcagaaggcg gccgaatcag tggtcaaggt ctccggcacc
     1621 tgcaacggct ttggcgtcat gggttccggg tgggcggtcg gacccgacac tttcgtgacc
     1681 aatgcccacg tggtggcagg ccaggacgac acgacgatcc agaccaatga c
//
LOCUS       DAOTIP010000503         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82272_length_1726_cov_3.112507, whole genome shotgun sequence.
ACCESSION   DAOTIP010000503 DAOTIP010000000
VERSION     DAOTIP010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82272_length_1726_cov_3.112507"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1434
                     /gene="mltG"
                     /locus_tag="P5138_09150"
     CDS             163..1434
                     /gene="mltG"
                     /locus_tag="P5138_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRV60785.1"
                     /translation="MSDRWFDDLPEHDPNDPDAVERALRRQERASRRQQEGSGKQARK
                     PVLGSGRSGGSRGSGGGRGSRGDRRRVGSGGPRTGRIVAVAVGAVGLIIVVWFLFALF
                     QPFKGDGDGEPFPLNVPAGDSVGQIGDLLADRGIISNSTLFELRATLSGKRGDLNHGV
                     HQFRENMSYSSAIDELTKTTSKRTITVTIPEGLSRSETAAVVKEAGIPGDYMAATVKV
                     KGFNPNDYGAQGRAENLEGFLFPATYELKPTADVEDLVAEQLQAFKDNIAGVNMKYAK
                     SKNLTVYDVLTIASMIEREVSVAKERKLVSAVIYNRLKMGEPLGIDATIRFALDNWTD
                     PLTETDLATDSPYNTRTNQGLPPGPIGNPGLASIEAAAHPADSKVWLYVVKPGTCGEH
                     VFSSSLAEHNRNVEKYQEAQAAAGGSPTDCG"
     gene            1454..>1726
                     /locus_tag="P5138_09155"
     CDS             1454..>1726
                     /locus_tag="P5138_09155"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRV60786.1"
                     /translation="MSPDPLRRLAVLGHPVSHSRSPAMQNAALAAAGLTGWEYEAIDV
                     EPGQFRGLVRKLPELGFVGVNVTVPHKEAALEIADEASGAAEAIGAA"
BASE COUNT          323 a          549 c          600 g          254 t
ORIGIN      
        1 agagacctgg gacgagcgct tcacgacccg catggccgcg cagacccggc gcgagacggg
       61 agccgggtcg gccgaagact cgatcgccgc ggcgcacctg ctggagagct acctcctggc
      121 ggcctcgagc cgggaaaacg cggacgggga ggaggccgga cagtgagcga tcgctggttt
      181 gacgacctcc ccgagcacga tccgaatgat ccggacgcgg tcgaacgcgc tctgagacgc
      241 caggagcggg catcccgccg gcagcaggag ggttcaggca agcaggccag aaagccggtg
      301 cttggcagcg gacgctccgg aggaagccgc gggtccggag gcggccgggg aagccggggc
      361 gaccggcgcc gggtcggttc cggtggcccc cggactggaa ggatcgtcgc ggtcgccgtc
      421 ggggcggtcg gcctgatcat cgtcgtctgg ttcctcttcg cgctctttca gcctttcaag
      481 ggcgacggtg acggtgaacc gttcccgctg aacgttccgg ccggtgacag cgtcggccag
      541 atcggcgacc tgctcgcgga ccgcgggatc atttccaact ccactctttt cgagttgcgg
      601 gcgacgcttt ccggcaagcg cggcgacctc aaccacgggg tccaccagtt ccgcgagaac
      661 atgtcctatt cctccgcgat cgacgagctg accaagacga catcgaaacg cacgatcacg
      721 gtgacgattc cggagggcct gagccgctcg gagacggccg cggtggtcaa ggaagcgggg
      781 attccgggcg attacatggc cgcgacggtc aaggtcaagg gattcaaccc caacgactac
      841 ggagcccagg gaagggcgga aaacctcgaa gggttcctct tcccggcgac ttacgagctg
      901 aagccgactg ccgacgtcga ggacctggtg gccgagcagc tccaggcctt caaggacaac
      961 atcgccgggg tcaacatgaa gtacgcgaag tcgaaaaacc tgaccgtcta cgacgtcctg
     1021 accatcgcct cgatgatcga gcgggaagta tcggtcgcga aggagcgcaa acttgtctcg
     1081 gccgtgatct acaaccggct gaagatggga gagcctctcg gaatcgacgc gacgatcagg
     1141 ttcgccctcg acaactggac ggacccgctg accgaaactg atctggccac cgactcgccc
     1201 tacaacaccc ggaccaacca gggacttcct ccggggccga tcggtaatcc ggggctggcc
     1261 tcgatcgagg cggcggccca tccggccgac agcaaggtct ggctctacgt ggtgaagccg
     1321 ggcacctgcg gggagcatgt cttctcgtcc tcgctggccg agcacaaccg gaacgtcgag
     1381 aagtaccagg aagcccaggc cgcggcgggc ggctcgccga ccgattgcgg atgaccatgg
     1441 ggagcgcgga ggcttgagtc cggatccgct caggcgactc gcggtactgg ggcatcccgt
     1501 ttcccactcc cgttcgccgg ccatgcagaa cgccgcgctg gcggcggccg gattgactgg
     1561 atgggaatac gaggcgatcg acgtggaacc cggtcagttc cggggccttg tccgcaagtt
     1621 gccggagttg ggcttcgtcg gagtgaacgt gaccgtcccg cacaaggagg cggccctgga
     1681 aatcgccgac gaggcctccg gtgctgccga ggcgatcggg gcggcc
//
LOCUS       DAOTIP010000504         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82338_length_1725_cov_3.295210, whole genome shotgun sequence.
ACCESSION   DAOTIP010000504 DAOTIP010000000
VERSION     DAOTIP010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82338_length_1725_cov_3.295210"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="P5138_09160"
     CDS             complement(<1..1675)
                     /locus_tag="P5138_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60787.1"
                     /translation="MPDYRLRLERISSAMTGLDPFVLVFDDAHLIQADSAWQVIYTVI
                     ASMPERSQVAVATRSHPGLPVGRLRARGELLELGVRDLALTRRESHELLASLDLDLGD
                     VADEIHDRAEGWPAALYLAGLAIRQAGGTKPEPQSFDGGDRIVVEYFRDEFFQDMPDE
                     KAQFLYRTSILEELTGPLCDAVTDRSDSAEMLNELAKENALIIPLDRTGDRFRCHHLF
                     RDMLLSEMRHRDPEAKSELRRRAAIWLAANGEIPRATDHAINSGDFELAGSLIWLDIA
                     DTLGHGRLASLDRWLGEIGDDRVAQIPALILARSHRELAMGHGDEAFYWLSVAEREIP
                     EDSPVYGDVILLRATMGPNGTEGMIEDGEMAAELMAPSNPFQVPSKLYRGIGLFLGED
                     ESAEPVLREAAREAVKVSAIIQVIAQAQLALEALDSGDLDRADEYASRARDVVERSGL
                     RILRIMSLVYVVLGLVRAQQGRVDDSMALLESGAKVGEGMVDFIPWYEGELAATRCRA
                     YLRTGQFDPATEQLRKAESVLESIPGATRLEGWIDDARDQLERARGGSSS"
BASE COUNT          300 a          579 c          527 g          319 t
ORIGIN      
        1 gtgaggaact cccaccccgg gcccgctcga gctgatccct tgcgtcgtcg atccagcctt
       61 ccagccgggt cgcaccggga atgctctcga gaacggactc ggcctttctg agctgctccg
      121 tcgccgggtc gaattgcccg gtccgcaggt aggcgcgaca gcgggtcgca gcgagttcgc
      181 cctcgtacca gggaatgaaa tcgaccatgc cctcaccgac tttggcaccc gattcaagca
      241 gggccattga atcgtcgact ctcccctgct gcgccctgac caatcccagg acgacatata
      301 cgagagacat gattcgcagg atcctgagcc ccgatcgctc gacaacgtcc cgggccctcg
      361 acgcatattc atcggctctg tcgagatcac ccgagtcaag cgcctcgagg gctagctggg
      421 cctgggcgat gacctggatg atcgccgaga ccttcacagc ctcccgggcg gcttcccgca
      481 ggacgggctc ggcagactca tcctcaccga ggaacaggcc gatcccccgg tagagcttgg
      541 acggcacctg gaatgggttg gacggggcca tcagttcggc tgccatttcc ccgtcttcaa
      601 tcatcccctc ggtgccgttt ggacccatgg tcgcccgaag caggatcacg tcaccgtaga
      661 ccggtgaatc ctccgggatt tccctttcgg cgaccgaaag ccagtagaac gcttcatctc
      721 catggcccat ggcgagttcc cggtgggacc gcgcgaggat cagggcggga atctgcgcga
      781 cccggtcgtc gccgatctca cccagccagc gatcgagcga agcgagcctc ccgtgaccga
      841 gagtgtcggc gatatccagc cagataagtg aaccggccag ttcgaaatca ccggagttga
      901 ttgcgtggtc ggtggcccgg ggaatttccc cgttcgcggc gagccagatc gccgcccggc
      961 ggcgaagctc tgatttcgct tccgggtcgc gatgccgcat ctcggagagc agcatgtccc
     1021 ggaacaggtg gtggcagcga aacctgtctc cggttcgatc cagcgggatt atcagggcat
     1081 tctctttggc cagttcgttc agcatctctg ccgagtcgct ccggtcggtg accgcatcgc
     1141 agagagggcc ggtcagctcc tccagaatcg aggtccggta gaggaactgg gctttctcgt
     1201 ccggcatgtc ctgaaagaac tcgtcgcgga agtactcgac gacgatcctg tcgcccccgt
     1261 cgaagctctg cggctcgggc tttgtccctc ccgcctgcct gatcgcgagc ccggccagat
     1321 agagggcggc cggccatccc tcggcccggt catggatctc gtcggccacg tctcccagat
     1381 caaggtccag gctggccagg agttcgtgac tctcccggcg ggtcagggcc agatccctga
     1441 cccccagttc gagcagttcc cccctcgccc tgagtcgccc gaccgggagg cctggatgcg
     1501 acctcgtggc gaccgcgacc tgtgagcgtt ccggcatgct cgcgatcacg gtgtagatca
     1561 cctgccaggc cgaatcggcc tggatcagat gcgcgtcgtc gaatacgagg acgaacggat
     1621 cgagtccggt catcgccgac gagatcctct cgagtcggag gcgatagtcc ggcaccggtg
     1681 ctctcagttc atccagcgtc ccggagtcga taccggtcac ttcga
//
LOCUS       DAOTIP010000505         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82479_length_1723_cov_3.436451, whole genome shotgun sequence.
ACCESSION   DAOTIP010000505 DAOTIP010000000
VERSION     DAOTIP010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82479_length_1723_cov_3.436451"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="P5138_09165"
     CDS             <1..879
                     /locus_tag="P5138_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRV60788.1"
                     /translation="IDPTNRTWIGEEPTYLPPVQIGETMRALGLGEVVASNNDNFPVG
                     ALVNGLTGWTDYTVISDANPLMVVPDSAKSAPPATLLGMLGMTGCTAYFGMLEIGEPK
                     EGETVVVSAAAGAVGTVAGQLAKLKGARVVGIAGGPEKCAWIVDELGFDAAVDYKADD
                     WRQQLKDATPDGIDVDFENVGGEIMEAVFARLNIGARVALCGLISGYNDDTRPVDPRV
                     FARLLIHRVKLQGFIILDYYARFGEAIAQLSGWVAEGRLKSEQTVVEGFENLPNALNM
                     LFKGENKGKLVVKIAD"
     gene            972..1349
                     /locus_tag="P5138_09170"
     CDS             972..1349
                     /locus_tag="P5138_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60789.1"
                     /translation="MNDSTGRRLAAGMLALTGVLHLALSPEYFGEQAYIGVLFVLGGI
                     ATLALAAWLWNSSQPVAWLGAAVVCAGMAVGFILSRTTGLPGFHEAEWELSGILSVLL
                     EVGVIGLAALALGSGRQTTAATT"
     gene            complement(1396..>1723)
                     /locus_tag="P5138_09175"
     CDS             complement(1396..>1723)
                     /locus_tag="P5138_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60790.1"
                     /translation="RFRAFRYELRCWREGEIPDLEFESGEPLALSRSDELAATLIDLT
                     AKVPLLIWGRDELRLGEMWNSNSVIAWLLLATGIEADRISPPHGGRAPGWDAGIEACR
                     LSPPRT"
BASE COUNT          309 a          572 c          551 g          291 t
ORIGIN      
        1 atcgacccga ccaaccggac ctggatcggc gaggagccga cgtatcttcc gccggtgcag
       61 atcggcgaga cgatgagagc gctcgggctc ggtgaagtcg tcgcttccaa caacgacaat
      121 ttcccggtcg gcgccctggt caacggactg accggctgga ccgactacac ggtgatcagc
      181 gatgcgaacc cgttgatggt ggtgccggac agcgcaaaga gcgcgccgcc ggccaccctg
      241 ctcggcatgc tggggatgac cggatgcacc gcttacttcg gaatgctgga gatcggcgag
      301 cccaaggagg gcgagactgt ggtcgtttcg gccgcggcag gggcggtcgg aaccgtggcc
      361 ggccagctgg ccaagctcaa gggagcccgg gtggtcggca tcgccggcgg accggagaaa
      421 tgcgcctgga tcgtcgacga actgggcttc gacgcagcag tggactacaa ggccgacgac
      481 tggcgccagc agctcaagga cgccaccccg gacggcatcg acgtcgactt cgaaaacgtc
      541 ggcggggaga tcatggaggc ggttttcgcc cgtctcaaca tcggtgcccg ggtcgctctc
      601 tgcggactta tctccggata caacgatgac acccgtccgg tcgacccccg ggtcttcgcc
      661 cgcctgctga tccaccgggt caagcttcag ggcttcatca tcctcgacta ctacgcccgt
      721 ttcggcgagg cgatcgcgca gctgtccggg tgggtggccg agggcaggct gaagtccgaa
      781 cagaccgtgg tcgaaggctt cgagaacctg cccaatgccc tcaacatgct cttcaagggc
      841 gagaacaagg gcaagctcgt ggtcaagatc gccgactgag cttcttcaga gatttctaac
      901 gccgccctca cccggctctg accgggagtc gccaacgtgg gattccgaag tccaatgacg
      961 aaaggaattt catgaacgat tcgacaggga gacgtctcgc cgccgggatg ctggcgctga
     1021 ccggcgttct ccatctcgca ctctcacccg aatatttcgg agagcaggcc tacatcgggg
     1081 tcctcttcgt acttggcggc atagcgaccc tcgcgctcgc cgcctggctg tggaacagca
     1141 gccagccggt ggcctggctc ggtgccgcag tcgtctgcgc cggcatggcc gtcggtttca
     1201 tcctctccag gaccacgggg ctgcccggct tccatgaagc cgagtgggag ctctcgggga
     1261 tcctctccgt cctgctcgaa gtcggtgtga tcggactggc agcgctcgct ctcggctcgg
     1321 gtcgccagac caccgcagcc acgacctgac catccggcca gcgaagtcat ccggccatct
     1381 ccgccccggc gcagttcagg tccggggcgg agacagccgg caggcttcga tgcccgcgtc
     1441 ccagcccggc gcccggccgc catgcggcgg tgaaatccgg tccgcttcga taccggtggc
     1501 aagcaggagc caggcgatca cagaattcga attccacatc tcgcccagcc tcagttcgtc
     1561 ccggccccag atgagcaacg gcaccttcgc ggtcagatcg atcagcgtgg cggcaagttc
     1621 gtccgatctg ctcagggcca agggctcccc ggattcgaat tccaggtcgg ggatctcccc
     1681 ctcgcgccag catctcagtt cgtaacggaa ggcccggaac cgc
//
LOCUS       DAOTIP010000506         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82508_length_1723_cov_2.419065, whole genome shotgun sequence.
ACCESSION   DAOTIP010000506 DAOTIP010000000
VERSION     DAOTIP010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82508_length_1723_cov_2.419065"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="P5138_09180"
     CDS             complement(<1..705)
                     /locus_tag="P5138_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRV60791.1"
                     /translation="MKAAVVQINSGADRSRNIGKAGSLVAAAADEGAELVVLPEKWSL
                     LGDGAALAEGAESSEGEAVTAARGWARDLGISLLAGSFTETSEDGLPTNTSVLISPDG
                     GIAAEYRKLHMFDVEAGGVRYRESEHEQAGGEIATGDAGDLKIGLTICYDLRFPELFR
                     ALLDRGVNAFTVPSAFTSATGRDHWEPLLRARAIENQAFVLAANQTGEADPSYDSWGH
                     SMIVGPWGEVLAEAGEG"
     gene            complement(768..>1723)
                     /locus_tag="P5138_09185"
     CDS             complement(768..>1723)
                     /locus_tag="P5138_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRV60792.1"
                     /translation="PPSQMPIQIRKDFGSNMNDSYWLSNPNAPLEGYPSIIGNEQSAR
                     SLRTRNGLHQITTRLNGTDGDPGNKFTIDRVREFTTNNKNYGAEVLVPGMVSYCQSHP
                     MIDSVDVSGACNVLANWDMTEKLDSPGAYLGRELLGNLLSVSGGPFATPFSLSDPVNT
                     PSGLDTSNPEVPSALADAVTYMNSKSIPLDASWRDYQYVTKNGEQIPIPGGFGGQGMF
                     NVISAVRNSSTGVYDSVRHGSSFIITASLTGAKCPDVKTILTYSQAATNEKSPHYADQ
                     TKLFSNSGWVTDRFCPSQQKKSPGLKVANLNGGAKAVKKGW"
BASE COUNT          278 a          542 c          563 g          340 t
ORIGIN      
        1 cccttcaccc gcctcggcga ggacttcgcc ccagggtccg acgatcatcg aatgacccca
       61 ggagtcgtat gaggggtcgg cctcgccggt ctggttggcg gccagcacga aggcctggtt
      121 ctcgatcgcc cgggcccgga gcaggggctc ccagtggtcc ctgccggttg cgctggtgaa
      181 agccgagggc acggtgaaag cattgacgcc gcggtcaagc agggcccgga aaagctcggg
      241 gaagcgcagg tcgtaacaga tggtgaggcc gatcttcagg tctccggcgt ctcccgtggc
      301 aatttccccg ccggcctgct cgtgctcgga ttcgcggtac cggacaccac cggcttcgac
      361 gtcgaacatg tggagcttcc ggtactcggc ggcgatcccg ccgtccgggg agatcagaac
      421 cgatgtgttg gtcggcaggc cgtcttccga ggtctcggtg aagctgcctg ccagcaggct
      481 gatgcccaga tccctggccc atccgcgggc ggcggtaacc gcctcgcctt cgcttgactc
      541 ggctccttcg gcgagggccg cgccatcgcc cagcagtgac catttttccg gcaggaccac
      601 cagttcggcc ccttcgtccg cggccgcagc gaccagcgag cctgccttcc cgatgttgcg
      661 ggaccggtca gctccggaat tgatctgtac gacggcggcc ttcatctgtg ggggttgatt
      721 gacggatcaa tcaaccccca cagactaccg gcaagccggg gccgggatta ccagcccttc
      781 ttgaccgcct tggcgccacc gttcaggttg gcgaccttga ggcctggtga tttcttctgc
      841 tgcgacgggc agaatcgatc ggtgacccag ccgctattcg agaagagctt cgtctggtcc
      901 gcgtaatgcg gcgacttctc gttggtggct gcctgcgagt aggtcaggat cgtcttcaca
      961 tccggacact tcgcaccggt cagtgaggcc gtgatgatga agctcgagcc gtggcgcacg
     1021 ctgtcgtaga cgccggtgct cgaattcctc accgcggaga tcacgttgaa catgccctga
     1081 ccaccgaagc cgccggggat cggaatctgc tcgccgttct tggtcacgta ctggtagtcg
     1141 cgccaggaag cgtcgagcgg aatcgacttg ctgttcatgt aggtaaccgc gtccgcgagg
     1201 gccgagggca cttccggatt ggaggtgtcc aggccgctcg gggtgtttac cgggtcactc
     1261 agcgagaacg gagtcgcgaa cggtccgccg ctcaccgaca gcaggttgcc gagcaactcg
     1321 cggccgaggt aggcacccgg cgaatcgagc ttctcggtca tgtcccagtt cgccagcacg
     1381 ttgcaggcgc cactgacgtc gaccgaatcg atcatcggat gcgactggca gtagctgacc
     1441 atgccgggca cgagcacctc ggcgccgtag ttcttgttgt tggtggtgaa ctcgcgcacc
     1501 cggtcgatcg tgaacttgtt gcccggatca ccatcggtgc cgttcagcct ggtggtgatc
     1561 tgatgcaaac cgttcctggt cctgagtgac cgggccgact gctcgttgcc gatgatgctc
     1621 gggtaaccct cgagcggagc gttcgggttc gacagccagt agctgtcgtt catgttcgac
     1681 ccgaagtcct tgcggatctg gatcggcatc tggctgggag gca
//
LOCUS       DAOTIP010000507         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82734_length_1720_cov_1.545946, whole genome shotgun sequence.
ACCESSION   DAOTIP010000507 DAOTIP010000000
VERSION     DAOTIP010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82734_length_1720_cov_1.545946"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..655
                     /locus_tag="P5138_09190"
     CDS             152..655
                     /locus_tag="P5138_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRV60793.1"
                     /translation="MKKELEAGSSRNGRVSAGADMPTVQNLDAARAASFDEGLKRRAN
                     NGLPLTEAGLLTRAVRIIQLRHEGKPDSEIIRQLHLGGADELEQDLARYREKVAAKAA
                     SRIGGLSRDVELDKAILERLKDHPGSRKELAKELGISYSALVSRITRLRRDGHEIPDG
                     RYRMLAA"
     gene            756..962
                     /locus_tag="P5138_09195"
     CDS             756..962
                     /locus_tag="P5138_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60794.1"
                     /translation="MNDPQKARSHISCVVTTRDQSRVYYDADQASTFVLTHPGQIKSI
                     FPSSMPMFGRTAESGPATKEGTDE"
     gene            955..1227
                     /locus_tag="P5138_09200"
     CDS             955..1227
                     /locus_tag="P5138_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60795.1"
                     /translation="MSDTTITLPEITDEFAGYPVGVEENPSGGWLVMNGDTWWGIAVR
                     NYLPAAQAIARQANGMDPKPGDAYLLSLEPVGNEYGPNREGACVVM"
     gene            1212..1577
                     /locus_tag="P5138_09205"
     CDS             1212..1577
                     /locus_tag="P5138_09205"
                     /inference="COORDINATES: protein motif:HMM:NF022619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HRV60796.1"
                     /translation="MRSHVTQTDPVFSGEAYEYLLESDQLPSYPELGGGDPVAKVRLF
                     LPGSRFEYFVTALTDYDGQLVMTGFCVSPLGPDCDEEGDLAVAELIELRGPDGLPVER
                     DCHWSPKSLTEIRKEISNA"
BASE COUNT          392 a          565 c          501 g          262 t
ORIGIN      
        1 tcccgggaac gagccaaccc caatccgggt gatttgaatg aacgccgcac gcacacgcat
       61 caggcacagg gaatgccatg cccctgactg tcaaggctcc gcgatcgacg gaggaatctt
      121 ctgtcggctc tgccaggcct tgcttgaccg cgtgaaaaag gaactcgaag ccggatcctc
      181 ccgcaacgga cgggtttcgg ctggcgctga catgccaacc gtccagaacc tggacgcggc
      241 aagggcagcg agcttcgacg aaggactcaa acgacgggcc aacaacggcc tgccgttaac
      301 cgaggccggg ctccttacgc gtgcggtgcg aatcatccag ctcagacatg aaggaaaacc
      361 tgacagcgaa atcatcaggc aactccatct cggtggcgcc gatgaactcg aacaggacct
      421 tgcccgctac cgggagaagg tggccgccaa agcagcatca cgcattggcg gtctcagccg
      481 agacgtcgag ctcgacaagg cgatccttga acgtctgaaa gatcaccccg ggtcacggaa
      541 agaactcgcg aaagaactcg ggatctccta cagcgccctc gtctcccgga tcacccggct
      601 aaggcgagac ggccacgaga tcccggacgg ccgctaccgc atgctcgcgg cctagaaaac
      661 cctgaacaaa cgaagcgtcc cggaagccgg ccatgtgccg gcttccgacg tttccggccg
      721 gtcacgaacc ggtcctgaaa ctccgaaagg acaacatgaa cgatccacaa aaggcccgca
      781 gtcacatcag ctgcgtggtc acgacacgag atcagtcccg tgtctactac gacgccgatc
      841 aggcaagcac attcgtgctc acccatcccg ggcagatcaa atcgatcttc ccgtcgtcga
      901 tgccgatgtt cggccgcacc gccgaatccg gtccggcaac caaggagggc accgatgagt
      961 gacacaacca tcaccctgcc cgaaatcacc gacgagttcg ccggctaccc ggtcggggtg
     1021 gaagaaaacc cgtccggtgg ctggctggtc atgaacggtg acacctggtg gggcatcgcg
     1081 gtccgcaact acctgccggc cgcgcaggca atcgcccgcc aggcaaacgg catggacccg
     1141 aaacccggtg acgcatatct gctgtcactg gagccggtcg gcaacgagta cggcccgaac
     1201 cgggagggcg catgcgtagt catgtaaccc aaaccgaccc tgtcttcagc ggggaagcct
     1261 acgagtacct gctcgaaagc gaccaacttc ccagctaccc cgaactcggt ggcggagacc
     1321 cggtcgcgaa agtccggctg tttttgccgg gcagccggtt cgaatacttc gttaccgcgc
     1381 tcaccgacta cgacggccag ctggtcatga ccggattttg cgtcagtccg ctcggccccg
     1441 actgcgacga ggaaggcgac ctggcggtcg cggagctgat cgagcttcgc ggccctgacg
     1501 gcctgcccgt tgaacgggac tgccactggt ccccgaaatc actcaccgaa atcaggaagg
     1561 aaatctcaaa tgcctgagat cgttttcatc cgggtcggct tcgacgacaa gccggccgca
     1621 ccaaaaatca acaccccctg ggggaccgct gactatgttg aggagctggc agacggaatc
     1681 atccaggtcg gcaccccgac ccacggaggg atcggggtca
//
LOCUS       DAOTIP010000508         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82988_length_1716_cov_2.700783, whole genome shotgun sequence.
ACCESSION   DAOTIP010000508 DAOTIP010000000
VERSION     DAOTIP010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82988_length_1716_cov_2.700783"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5138_09210"
     CDS             complement(<1..550)
                     /locus_tag="P5138_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRV60797.1"
                     /translation="MRKRTRGIALASLASLAALVGYDVSQRRKPILRNFPVIGHFRYL
                     LEAFGPELRQYIVTSNNEERPFSRDQRRWIKTSSEGKPNVFGFGTDDEVESVDSLVII
                     KHSPFPAPAPGDGEPGGAPDFEVPAAKTLGAAHGRKHAFRPASVVNISGMSFGALSPP
                     AVEALNRGAAIAGCLHNTGEGGI"
     gene            complement(676..855)
                     /locus_tag="P5138_09215"
     CDS             complement(676..855)
                     /locus_tag="P5138_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60798.1"
                     /translation="MDPDSDISYIPAIIGFFVAIIAIIASFMFLGGVVGMIVLIVFLM
                     IALYIGYRLLSQNDG"
     gene            1012..>1716
                     /locus_tag="P5138_09220"
     CDS             1012..>1716
                     /locus_tag="P5138_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HRV60799.1"
                     /translation="MTDTKNSEPLGRRLLGISSDKTRTPEEIRDAVYLSHGDVAAKGS
                     RFWLLLVLSAGIATAGVISDSTATVIGAMIIAPLATPIQGVAVAITFGEVRPLIASVT
                     TLALAMAVVIATGFLLALVLPELVPISQNSQITGRVSPTIVDLVAAAVTGVAGSFAIA
                     RRDIGDILPGVAIAISLVPPLAVVGVTAQGHDWDGALGALVLFATNMLAIIVVGVIVF
                     SGVQLIRGGRPQPAISS"
BASE COUNT          298 a          537 c          582 g          299 t
ORIGIN      
        1 cgattccgcc ctcaccggtg ttgtgaaggc aaccggcgat tgctgcaccc cgattgagtg
       61 cctcgaccgc cggcggggag agcgcgccga aactcatccc cgagatattc acgaccgagg
      121 cggggcggaa agcgtgtttc cgtccgtgcg cggcacccag cgttttcgcc gccgggactt
      181 cgaagtcagg cgcgccaccg ggttccccgt cgcccggggc gggagccgga aagggcgagt
      241 gcttgatgat caccagactg tccaccgact cgacctcgtc gtccgtgccg aagccgaaaa
      301 cgttcggttt gccttcggag gaagtcttga tccagcgccg ctggtcccgg gagaaaggcc
      361 gctcctcgtt gttggaggtg acgatgtact ggcgcaactc ggggccgaat gcctcgagca
      421 ggtagcggaa gtggccgatt accgggaagt tgcgaaggat cggcttgcgc ctctgggaga
      481 cgtcgtagcc gacgagagcc gccagagagg ccagagaggc aagggcgatt cctctcgttc
      541 gcttgcgcat gcgagaaaac ctattgggtc acggccccgg cgtgggccgg gcggcagcag
      601 aacgggccgg cacgcaagag ccagcccgtt cccgagaccg caatatcggg ggtgcggtca
      661 agcgttggag gcttgtcagc cgtcgttctg gctgagcagc cggtaaccga tgtagagggc
      721 gatcatcagg aagacgatta gaacgatcat gccgaccacg cctccgagaa acatgaagga
      781 agcgatgatc gcgatgatcg cgacgaagaa gccgatgatg gccgggatgt agctgatatc
      841 cgagtctggg tccacgggtg gatcatgcca gcccgaccgg acccgaatct cactcgatgg
      901 aggtgaactt cgtgccgccc cggcccccgg cccgtcggcc ggcaccgacg gcttgggatg
      961 acgtcctgcg ctcctgctgg aatttgccct ggaaccggaa ccggattcga cgtgaccgac
     1021 accaagaaca gcgaaccgct gggccggaga ctgctcggca tttccagcga taagacccgg
     1081 actcccgagg agatccggga cgcggtctat ctgtctcacg gcgatgtcgc cgccaaaggg
     1141 agcaggttct ggctgctcct ggtgctctcg gccgggatcg cgaccgccgg ggtgatctcc
     1201 gattcgaccg cgacggtcat cggggcgatg atcatcgctc cgctcgcgac gccgattcag
     1261 ggggtggcgg tcgcaatcac cttcggcgag gtccggccgc tgatcgcttc tgtgacgacc
     1321 ctggccctgg ccatggcggt ggtgatcgcc accggattcc tgttggccct ggtgctgcct
     1381 gagctcgtgc caatttcgca gaactcgcag atcacgggaa gggtttcacc cacgatcgtc
     1441 gacctcgtgg cagctgctgt taccggagtg gccggatcct tcgcgatcgc cagacgggac
     1501 atcggagaca tcctgccggg cgtggcgatc gccatctcgc tggttccgcc gctggcggtg
     1561 gtcggagtca cggcccaggg ccatgactgg gacggagccc tcggcgccct ggtgctcttc
     1621 gccaccaaca tgctggcgat catcgtggtc ggggtgatcg tcttcagcgg cgtccagctg
     1681 attcgtggcg gccgccccca acccgcgatc agctcg
//
LOCUS       DAOTIP010000509         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_82998_length_1716_cov_2.520771, whole genome shotgun sequence.
ACCESSION   DAOTIP010000509 DAOTIP010000000
VERSION     DAOTIP010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82998_length_1716_cov_2.520771"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="P5138_09225"
     CDS             complement(<1..692)
                     /locus_tag="P5138_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRV60800.1"
                     /translation="MNWFDGRLAAFDIETTGTDIENDRIVTAAVSIVGGGEGTVSRNW
                     LVNPGVEIPAEATEVHNITNEMARRDGVATVEAVEEITSLLAGALEEETPVIAFNARF
                     DLTILDREARRHGVLPLIDRVGGPDGLLVVDPYVVDRHYNKYRRGRKNLAILCEAFGV
                     PLEGAHAADADALAAVRLAWRMASRNEELGQMELFELHEHQIHWAEEQARDLKEFFEK
                     NGRNDHVEGAWPV"
     gene            complement(723..>1716)
                     /locus_tag="P5138_09230"
     CDS             complement(723..>1716)
                     /locus_tag="P5138_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV60801.1"
                     /translation="QPQTVAGHAGLRLAVTVGHEHEHGEGGHSHGPGAWRDADRKALL
                     IAAGLTAGFMFAEIIGGLLTDSLALLADAGHMLSDSFSLFLALGAVALAARPATPRRT
                     YGYKRAEILAALINGVLLVVVSVWIVIEAIGRIGDPPDVLGGWMLFVAAIGLMVNLIA
                     AWVLYRSAGESLNIKAALRHVLADVAGSIGVILAALIILTTGWELADPIVSILISVLI
                     AASAWSILRDSVDVLLEAAPPGLDTEEIGYSMAGLKGVDQIHDLHIWQITSGMPMLSA
                     HVLVGPDSDCHGIRRELEELLRREFEIDHTTLQVEHTGRQEPITIEEMGRSSQI"
BASE COUNT          294 a          562 c          554 g          306 t
ORIGIN      
        1 accggccagg caccttcgac atggtcgttg cggccgttct tctcgaagaa ctccttcaga
       61 tcacgggcct gttcttctgc ccagtggatc tggtgttcgt gcagctcgaa aagctccatc
      121 tggcccagtt cctcgttccg tgaagccatc ctccaggcca gccgcactgc cgccagcgca
      181 tcggcgtcag ccgcgtgggc accctccagc ggaaccccga atgcctcaca gaggatcgcc
      241 agattcttcc ggccccggcg gtacttgttg taatgcctgt caaccacata cgggtccacg
      301 accagcagac catccggccc gccgacccgg tcaatcagcg gcagaacccc gtgccgacgg
      361 gcttcccgat cgaggatcgt gagatcgaaa cgggcgttga aagcgattac cggggtctct
      421 tcttcgaggg cgcccgccag cagcgaagtg atctcctcca ctgcctcgac ggtggctacg
      481 ccgtcacgac gcgccatttc gttggtgatg ttgtgaacct cggtggcttc ggccgggatc
      541 tcgacgccgg ggttgaccag ccagttgcgg ctgacggtcc cttcgccgcc gccgacgatg
      601 ctgacggcag cggtgacgat ccgatcgttc tcgatgtcgg ttccggtggt ttctatgtcg
      661 aaggcggcca gtcgcccgtc aaaccagttc acgattcaga aggtagcgtc ggccccggac
      721 gctcagatct gactcgaacg gcccatctct tcaatcgtga tcggctcctg acggccggtg
      781 tgctcgacct ggagagtcgt gtggtcgatc tcgaattcgc gccgcagaag ctcttcgagt
      841 tcccggcgga tcccgtggca gtccgagtcc ggtccgacca ggacgtgggc gctcaacatc
      901 ggcatgccgg aggtgatctg ccagatgtgg aggtcgtgga tctggtcgac ccccttcagc
      961 ccggccattg agtagccgat ctcctcggtg tcgaggccgg gaggggcggc ctcgagcagg
     1021 acgtcgaccg agtcacggag gatcgaccag gcactggccg cgatcaggac cgagatcagg
     1081 atcgagacga tcgggtcggc cagctcccag ccggtggtga ggatgatcag ggcggcaagg
     1141 atcacgccga tcgacccggc gacgtcggcc agaacgtgac ggagggcagc cttgatgttc
     1201 agactttccc ccgccgaccg gtagagcacc caggcggcga tgaggttcac catcaggccg
     1261 atcgcagcga cgaagagcat ccagccgcca agcacgtcgg gcggatctcc gatcctgccg
     1321 atcgcctcga tcacgatcca gaccgagacc accaccagca gcacgccgtt gatcagggcg
     1381 gcaagtattt cggcgcgttt gtacccgtat gttcgtcggg gcgtcgccgg cctggcggca
     1441 agcgccacag cgccgagggc caggaacagc gagaagctgt cggagagcat gtgaccggcg
     1501 tcggccagca gggccagcga gtcggtgagg aggcccccga tgatctccgc gaacatgaat
     1561 cctgcggtga gccccgctgc gatgagcagg gctttccggt cggcgtcgcg ccaggccccc
     1621 ggtccgtggc tgtgtccgcc ttcgccgtgt tcgtgctcgt gacccaccgt gactgcgagt
     1681 ctaagaccag cgtggccggc caccgtttgc ggctgg
//
LOCUS       DAOTIP010000510         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_83379_length_1710_cov_4.042900, whole genome shotgun sequence.
ACCESSION   DAOTIP010000510 DAOTIP010000000
VERSION     DAOTIP010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83379_length_1710_cov_4.042900"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="P5138_09235"
     CDS             <1..948
                     /locus_tag="P5138_09235"
                     /inference="COORDINATES: protein motif:HMM:NF005750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HRV60802.1"
                     /translation="ERELVDSDVVISQPFWPAYLTAERIASAPNLRLAITAGIGSDHV
                     DLQAAMDAGITVAEVTYCNSISVAEHVAMSILVLVRNFVPSHQIVLDGGWNIADAVSR
                     SYDLEGMQVGTVAAGRIGLGVLRRLAPFDVGLHYMDRYRLPAEVEEELNLTHHDTVES
                     LVEVCDVVTINCPLHPETEHLFDAEMIGRMKRGAYLINTARGKICDRDAVAAALESGH
                     LAGYAGDVWFPQPAPADHPWRQMPNNGMTPHISGSSLSAQARYAAGTREILECWMEEK
                     PIRDEYLIVESGNLAGTGAHSYSAGNATSGSEEAARFKK"
     gene            complement(973..>1710)
                     /locus_tag="P5138_09240"
     CDS             complement(973..>1710)
                     /locus_tag="P5138_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV60803.1"
                     /translation="RRSAAEESRTIVANATHGTLGSLSDDGTPWASLVTYGVMDDGSP
                     VFCLSTLAEHARNLEKNRQASLMVADPFHKGEVLAGGRVTLAGNVKRSDEHPDESSAR
                     AAHLEAVPSAAMYADFNDFSFWILEVDRVRWVGGYGRMDSATDEDYRNAEPDPVQPQV
                     PDAIAHLNADHPDALLLMAQAFIDLPEITEASCERADRYGLDLQLVTPEGDKAGRVSF
                     TDRIDTPGGLRAATVDLARRARQKLGA"
BASE COUNT          277 a          569 c          588 g          276 t
ORIGIN      
        1 gagcgggaac tggtcgattc ggatgtcgtc atctcccagc cgttttggcc ggcttacctg
       61 accgccgagc ggatcgcttc ggccccgaac ctgaggctgg cgatcaccgc cggcatcggc
      121 tccgatcacg tcgacctcca ggcagcgatg gatgccggga tcaccgtggc cgaagtcacc
      181 tattgcaaca gcatcagcgt ggccgaacac gtggcgatgt cgatcctggt cctggtccgg
      241 aacttcgtgc cgtcccatca gatcgtgctc gacggaggct ggaacatcgc cgacgcggtc
      301 tcccgctcgt acgacctgga ggggatgcag gtcggcaccg tcgccgccgg ccggatcggc
      361 ctcggagtgt tgcgccgcct cgccccgttc gacgtcggac tccattacat ggacaggtac
      421 cggctcccgg cagaggtgga ggaagaactc aatctgaccc accacgacac ggtcgaatca
      481 ctggtcgagg tctgtgacgt agtgaccatc aactgtccgc ttcatccgga aaccgagcat
      541 ctcttcgatg ccgagatgat cggtcgcatg aagcggggcg cgtacctgat caacacggcc
      601 aggggcaaga tctgcgatcg cgacgcggtc gccgccgcgc ttgaaagcgg gcatctggcc
      661 ggttacgccg gcgacgtctg gttcccgcag ccggctccgg cggaccaccc gtggcggcag
      721 atgccaaaca acggcatgac cccgcacatc tccggttcgt cgctttcggc ccaggccagg
      781 tacgcggccg gcacgcgcga gatcctcgaa tgctggatgg aagagaagcc gatccgcgac
      841 gagtacctga tcgtcgagag cggcaacctg gccgggaccg gcgcccactc ctacagcgcc
      901 ggcaacgcca cctccggctc cgaggaggcg gccaggttca agaaatgagg acggggcggg
      961 cccgcccggc cgtcaggcgc cgagcttctg gcgggcgcgg cgggccaggt cgaccgtggc
     1021 ggcccggagg ccgccggggg tgtcgatccg gtcggtgaaa ctgacccggc cagccttgtc
     1081 cccctcgggc gtgaccagct gcaggtcgag cccgtagcgg tcggcccgct cgcagcttgc
     1141 ctcggtgatc tccggcaggt cgatgaaggc ctgggccatc aggagcaggg cgtcgggatg
     1201 gtcggcgttc aggtgcgcga tcgcgtccgg cacctgcggc tggaccgggt cgggctcggc
     1261 attccggtaa tcctcgtcgg tggccgagtc catgcggccg tagccgccga cccagcgcac
     1321 acggtcgacc tcgaggatcc agaaggagaa gtcattgaag tccgcgtaca tcgctgccga
     1381 cggcactgcc tcgaggtggg ctgcccgggc ggaagactcg tccggatgct catccgaacg
     1441 cttgacattg ccggccaggg tgaccctccc gccggccagt acctcgccct tgtggaaggg
     1501 gtcggcgacc atcagcgagg cttgccggtt cttctccagg ttccgcgcat gctcggccag
     1561 ggtcgagagg cagaagaccg ggctgccgtc atccatcacc ccgtaggtga ccagcgaggc
     1621 ccagggggtg ccgtcgtcac tgagtgaacc gagcgttccg tgggtcgcgt tggcgacgat
     1681 cgtgcgggac tcttcggcgg cgcttcggcg
//
LOCUS       DAOTIP010000511         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_83750_length_1705_cov_2.942424, whole genome shotgun sequence.
ACCESSION   DAOTIP010000511 DAOTIP010000000
VERSION     DAOTIP010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83750_length_1705_cov_2.942424"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="P5138_09245"
     CDS             <1..519
                     /locus_tag="P5138_09245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRV60804.1"
                     /translation="VNGIGSSKRVVIYDNAIRDLTPAEFSALIAHELGHVEADDLRRG
                     LTFALLVIPLGVLFVQMATGAVMCRNGDDPRGPALVPVLALWMTLAVFVLQVPGNALS
                     RQVEAKADRFSVSLTGDPQGLVDLQVGLAKANLSDVDPPSLWQFLFGTHPSTLDRIAI
                     AEGAKVSVRNES"
     gene            509..955
                     /locus_tag="P5138_09250"
     CDS             509..955
                     /locus_tag="P5138_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRV60805.1"
                     /translation="MKVELIAVGKIKPPFTEAEGHYLKMLKPLMPVAVTEVKDDEALG
                     RKVTAARATGGNSPDPGTVVALEAGGRDMTSERWAEWLDQRRHEGRRLTFLIGGPEGI
                     PGEVSGMADHRLSLGPQTMAHQLARVVLLEQLFRASKILAGQKYHL"
     gene            979..>1705
                     /locus_tag="P5138_09255"
     CDS             979..>1705
                     /locus_tag="P5138_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRV60806.1"
                     /translation="MTSSSDPTAALLDAVTVTARRLAGDDGVSPALDPSPRPEMGDYS
                     TNAAMMLAKPLGRPPREIAAELATSIEEELGDALEKTEIAGPGFINLFLGDAWYREAA
                     AAMASTGAAAVPPVPESERIIVEFVSANPTGPLTAASGRHAAYGDSLSRILRAAGNAI
                     TTEYYVNDGGTQVENFAASIAARMTGDEPPEDGYKGTYVNELAERAKAEGHEPADLER
                     LARTGVAWMVEGIRGSLENFGVEM"
BASE COUNT          286 a          574 c          564 g          281 t
ORIGIN      
        1 gtgaacggga tcggctcctc caagcgggtg gtcatctacg acaatgcgat ccgggatctg
       61 actccggccg agttctcggc tctcatcgcc catgagctcg gccacgtgga ggcggatgac
      121 cttcggcgcg gcctgacctt cgccctgctg gtgattccgc tcggagtgct ctttgtccag
      181 atggcgaccg gcgcggtcat gtgccggaac ggcgacgacc ctcggggtcc ggctctggtc
      241 ccggtgcttg ccctctggat gactctcgcg gtgttcgtgc ttcaggtacc cgggaatgcc
      301 ctctcgcgcc aggtggaggc gaaggccgac cgtttttcgg tcagcctcac cggcgacccc
      361 caggggctgg tcgacctgca ggtcgggctg gccaaggcga acctttccga tgtcgacccg
      421 ccgtccctct ggcagttcct cttcggaacc catccgtcga ccttggaccg gatcgcgata
      481 gccgagggtg cgaaagtaag tgttcgaaat gaaagttgaa ctgatcgccg tggggaagat
      541 caaacccccg ttcacagagg ccgaaggcca ctacctgaag atgctgaagc cgctgatgcc
      601 ggtcgcggtg accgaagtca aggacgacga agcgctcggc cgcaaggtca ccgctgcccg
      661 ggcgaccggt ggcaacagtc ccgatccggg aacggtggtg gcgctggagg ccgggggccg
      721 ggacatgaca tccgagcgct gggccgagtg gcttgaccag cgccgccacg agggtcgccg
      781 gctgaccttc ctgatcggcg gccccgaagg catccccggt gaggtttcgg gcatggccga
      841 tcaccgcctg tcgctcggcc cgcagaccat ggcgcatcag ctggcccgcg tcgtgctgct
      901 cgagcagttg ttccgggcaa gcaagatcct ggccggccag aaataccacc tgtagccggc
      961 caaaatagaa tcgcctccgt gaccagttct tcagatccga ctgccgccct gctcgacgcc
     1021 gttaccgtga ccgcgcgacg gctcgcgggc gatgacggcg tgagtcccgc cctggatccc
     1081 tcgccccggc cggaaatggg cgactactcg accaatgccg cgatgatgct ggccaaaccg
     1141 cttggtcgtc cgccgcgtga gatcgccgcg gaactggcca cttcgatcga ggaagaactc
     1201 ggcgacgctc ttgagaagac cgagatcgcc ggccccggat tcatcaacct cttcctcggc
     1261 gacgcctggt accgggaggc cgccgccgcc atggcctcga ccggggccgc ggccgtgccc
     1321 cccgttccgg aatcggagcg gatcatcgtc gagtttgtct ccgccaaccc gaccggaccg
     1381 cttaccgcgg cctccggccg gcatgccgcc tacggcgatt ccctttcccg gattctgcgg
     1441 gccgcgggaa acgcgatcac gaccgagtac tacgtcaatg atgggggcac ccaggtcgag
     1501 aacttcgctg cctcgatcgc cgcccggatg accggagacg agccgcccga agacggctac
     1561 aagggcacct acgtgaacga actggccgaa cgggccaagg ccgagggcca cgaaccggcc
     1621 gacctcgaga ggcttgcccg gacgggggtc gcctggatgg tcgagggcat ccgcggttcc
     1681 ctggagaact tcggggtcga gatgg
//
LOCUS       DAOTIP010000512         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84224_length_1698_cov_3.620207, whole genome shotgun sequence.
ACCESSION   DAOTIP010000512 DAOTIP010000000
VERSION     DAOTIP010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84224_length_1698_cov_3.620207"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="P5138_09260"
     CDS             complement(<1..1200)
                     /locus_tag="P5138_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRV60807.1"
                     /translation="MTESLADGFDSVSFEQWSEAATKGDANFALMTLLENGVEAKWLY
                     TREDAIAPDPSGLPGKAPFTRGTRAGRHWQIRQEQTNPDRARANAEILEDLNGGVTEV
                     TLRFDRAAREGLAPGSSGFDDARGIDGIAISNLDQLSQVLDGVYLDMAGVALEAGAAA
                     PAAAALLASHWRENGVAPDAALGSFRYDPLGCLARTGELPCSPEDALAVAGGLAASTK
                     ADWPGVKSLAVDTGIYVEAGATASWELGIAIATGVEYLRAAVEAGLDPAAAASQIEFT
                     LSVGPDQFLEMSKFRAMRRLWARVLEEAGVADEDRHSSTYGKTSARMISAVDPWVNML
                     RVTTAAFAAGTGGADGVTVTPFDASIGQPGSLGRRIARNTQIILQDESSLSRIADPLA
                     GSWYGEEL"
     gene            1306..>1698
                     /locus_tag="P5138_09265"
     CDS             1306..>1698
                     /locus_tag="P5138_09265"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRV60808.1"
                     /translation="MKRERKQPPPLPVAPARFRLIRIKQDPLRAIVQRFVLATLLLLI
                     VTFITYFGRKGYVDNEHPDQLLTFIDSLYYATVTITTTGYGDIVPVTQSARLVTTLVV
                     TPIRVLFIVLLVGTTLQVLAEQSRFQFRL"
BASE COUNT          264 a          567 c          567 g          300 t
ORIGIN      
        1 cagctcctcg ccataccagg agccggccag cgggtcggcg atccggctga gcgaggattc
       61 gtcctgcagg atgatctggg tgttgcgcgc gatccgccgg ccaaggctgc cgggctgtcc
      121 gatcgacgcg tcgaaggggg tgacggtcac gccgtccgca ccgccggtgc cggcggcaaa
      181 ggcggcggtg gtgacccgga gcatgttcac ccacgggtcg acggcgctga tcatccgggc
      241 tgaagtcttg ccgtatgtgg atgagtgacg gtcttcgtcg gcaactccgg cctcctccag
      301 aacccgggcc cacagtcgcc gcatcgcgcg gaacttggac atttcgagga actggtcggg
      361 gccgaccgag agggtgaatt cgatctgcga tgccgcggcg gcggggtcga gcccggcttc
      421 gacggcggcc cggagatatt cgaccccggt tgcgatcgcg attccaagct cccagctcgc
      481 cgtggctccc gcttcgacgt agatgccggt gtcaaccgcc agcgacttca cccccggcca
      541 gtcggccttc gtggatgcgg caaggccacc ggcgacggcg agggcgtcct cgggggagca
      601 aggcagctcg ccggtgcggg cgaggcagcc gagcgggtca tagcggaagg agccgagggc
      661 cgcgtcgggg gcgacaccgt tctcgcgcca gtgtgatgcg agcagggcgg cggcggcggg
      721 ggcggctgcg ccggcctcga gcgccacgcc ggccatgtcg aggtataccc cgtccagaac
      781 ctggctcaac tgatcaaggt tcgagatggc gattccatct atcccgcggg catcgtcgaa
      841 cccgctgctt ccgggagcga gtccttcccg ggcggcacgg tcgaaacgca gggtgacctc
      901 ggtgactccg ccgttcagat cctcgaggat ctcggcattg gcccgggccc ggtccgggtt
      961 ggtctgttcc tggcggatct gccagtggcg gccggcccgg gttccccgcg tgaacggggc
     1021 cttgcccggc agtccggaag gatcgggggc gatcgcgtcc tcccgcgtgt acagccactt
     1081 tgcctcgacc ccgttctcaa ggagggtcat cagggcgaag ttcgcatcac ccttggtcgc
     1141 agcttcgctc cactgctcga aggaaacgga atcgaagccg tctgcgaggg actcagtcat
     1201 ggcaatccgg agagataaac acgggccttg cagggtacct gtccgggacc ggcttccgcc
     1261 ttgccaccgg ggaacgccct ctggcaggtt ctatgctgct gccacttgaa acgggagcga
     1321 aaacagccgc cgccgctgcc ggtagccccg gcccggttcc gcctgatccg gatcaagcag
     1381 gatcccctcc gggcgatcgt ccagcgattc gtcctggcga ccctgttgct gctgatcgtc
     1441 accttcatca cctatttcgg acgcaagggc tacgttgaca acgagcatcc cgaccagttg
     1501 ctcaccttca tcgattcgct ctactacgcg acggtcacga tcacgaccac cgggtacggc
     1561 gacatcgttc cggtcaccca gtcggcccgg ctggtcacca ccctggtggt caccccgatc
     1621 cgcgttctgt tcatcgttct gttggttggc actacgcttc aggtcttggc cgagcaatcg
     1681 agattccagt tccgcctc
//
LOCUS       DAOTIP010000513         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84289_length_1697_cov_3.885505, whole genome shotgun sequence.
ACCESSION   DAOTIP010000513 DAOTIP010000000
VERSION     DAOTIP010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84289_length_1697_cov_3.885505"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="P5138_09270"
     CDS             complement(<1..702)
                     /locus_tag="P5138_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRV60809.1"
                     /translation="MKVALLQGGRSLERGVSLRSGQRVTEALLDLGHEVDVMDLGPEL
                     VTRLENNRPDVVFIALHGTEGEDGTVQALLELLDIPYTGPGVAACKRCMDKSLAKYIL
                     REHSLPTPDWVTYSEAAIRDFGAASTFGAAVDRVGLPLVAKPASGGSSLGVKVVTELE
                     QIPNALITALSYDNRALLETWINGRELAVSVLGGDALPVVEAIPTDSNAYDFEARYEI
                     GGASFECPADLDDAET"
     gene            complement(699..>1697)
                     /locus_tag="P5138_09275"
     CDS             complement(699..>1697)
                     /locus_tag="P5138_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV60810.1"
                     /translation="DLITKTLIDPGDVIICDAPTYPGAIPTFCSYEADVRQIEMDADG
                     MRIDLLEEMIESLEAEGRKPKFIYSIPTFQNPGGVTLSLERRRRLVEIAREKEILVIE
                     DNPYGLLRFEGEPLPTLYSLDGGNYVAYLGTFSKILSAGLRIGWLVTPTPIREKVVLG
                     KQAADLCTSTLTQDFAGHFFQSGRWREYVDELTGIYRARRDVMISAMAEYFPHGATWN
                     KPAGGLFIWATMPDVLSSEDLLAKALSKNVAFVPGPNAYVDGRGASSMRLNFSGVKED
                     EIIEGIRRIGQVAREMLRLYETMTSTDEMKLPPRPSPDGGGSEMRLPPRPPEEDPS"
BASE COUNT          277 a          551 c          562 g          307 t
ORIGIN      
        1 tgtctccgca tcgtcgaggt ctgccgggca ttcgaagctg gccccgccga tttcgtagcg
       61 ggcctcgaag tcataggcgt tgctgtcggt cgggatcgcc tcgactaccg gcagcgcatc
      121 cccgccgaga accgaaacgg ccagttcacg gccgttgatc caggtttcga gcagtgcccg
      181 gttgtcgtag ctgagtgccg tgatcagcgc gttggggatc tgctccagtt cggtgaccac
      241 cttcaccccc agcgatgacc cgcccgatgc cggcttcgcg accagcggca atccgaccct
      301 gtccaccgca gcaccgaagg ttgatgcggc gccgaagtca cggatcgcgg cttccgaata
      361 ggtcacccag tccggggtcg gcaggctgtg ctcgcgcagg atgtacttgg ccaggctctt
      421 gtccatgcag cgcttgcagg cggcgacccc ggggccggtg taagggatgt cgagcagctc
      481 gagcagggcc tggaccgtgc cgtcctcgcc ttcggttccg tgaagggcga tgaagacaac
      541 gtcgggcctg ttgttctcga gccgggtaac cagttccggg cccaggtcca tcacatcgac
      601 ctcatggccc aggtcgagca gggcctcggt cacccgctgg ccggagcgca gtgaaacgcc
      661 ccgctcaagc gaacggccgc cctgaagcaa ggccaccttc acgatgggtc ctcctcggga
      721 ggccgcggcg gcagacgcat ctcgctcccg cctccatcag gtgatggccg cggcggaagc
      781 ttcatctcat ccgttgaagt catcgtctcg tagagccgca gcatctcgcg ggcgacctgg
      841 ccgatccgac ggatgccttc gatgatctcg tcctccttca ccccggagaa attgagccgc
      901 atcgaactgg ccccgcgacc gtccacatag gcattcggcc cgggtacgaa ggcgacgttc
      961 ttggagaggg ccttggccag caggtcctcg ctgctcagga cgtcgggcat ggtcgcccag
     1021 atgaagagtc cgccggcggg cttgttccag gtcgcgccgt gcgggaagta ctcggccatg
     1081 gcggagatca tcacgtcgcg ccgggcccgg tagatcccgg tcagttcgtc gacgtattcg
     1141 cgccagcggc cgctctggaa gaaatgcccg gcgaagtcct gggtcagggt cgaggtgcag
     1201 aggtcggcgg cctgcttgcc gagcacgacc ttctcccgga tcggggtcgg agtcaccagc
     1261 cagccgatcc tcagtccggc cgaaaggatc ttcgagaagg tcccgaggta ggccacgtag
     1321 ttgcccccgt cgaggctgta gagggtgggg aggggttcac cctcgaagcg gagcaggccg
     1381 taagggttgt cctcgatgac caggatctcc ttctccctgg cgatctcgac caggcgacgc
     1441 cgccgctcca gcgaaagcgt caccccgccg gggttctgga aggtcgggat cgaatagatg
     1501 aacttcggct tccggccctc ggcttcgagc gactcgatca tctcctcgag cagatcgatt
     1561 cgcatgccgt cggcgtccat ctcgatctgc cgcacgtcgg cctcgtagga gcagaaggtc
     1621 gggatcgctc cggggtaggt cggggcgtcg cagatgatca cgtcgccggg gtcgatcagc
     1681 gtcttggtga tcaggtc
//
LOCUS       DAOTIP010000514         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84420_length_1695_cov_4.889634, whole genome shotgun sequence.
ACCESSION   DAOTIP010000514 DAOTIP010000000
VERSION     DAOTIP010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84420_length_1695_cov_4.889634"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="P5138_09280"
     CDS             <1..228
                     /locus_tag="P5138_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60811.1"
                     /translation="LNEHVGRRIDMPVEETLASFAGRDALRGRTVRWAEGEGTASGID
                     REGNLLVESTAGCLSTLNAGEVHLSLPVDAA"
     gene            225..773
                     /locus_tag="P5138_09285"
     CDS             225..773
                     /locus_tag="P5138_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRV60812.1"
                     /translation="MSPGSDSPTLAPVESDGLPIDFTRPGVIWLDELPVPIEPTGTGK
                     AVARLREGRLVVGGGSRTQVLAAIDRWYRREARKRIEPLVVIEGERLGLEAKKVRIGN
                     QRTRWGSCSTSGTVSFNWRLVIGRPEALHYVVVHELIHLRHHNHSRAFWDALAEAFPD
                     FKREATWLRSNERELLKWKPRI"
     gene            791..>1695
                     /locus_tag="P5138_09290"
     CDS             791..>1695
                     /locus_tag="P5138_09290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRV60813.1"
                     /translation="MIEVGRASIRKTTTGVIFVLFALASAFAISHPAEASTRSNLNRA
                     LNQVMEVPNGPPGISMQIVKNGHSRYFRRGVANVRTGAKPRLRERFRIASVAKAFSGA
                     VALNLVARGKLSLDSTIGEVLPGMMPKARQVTLGQALQHTGGLPEYIKSKGFIRDINR
                     NPAAFVPPTRIISWVRNSGLTHRPGTKYEYSDTDNIVVGLMAQRVTGVRYLWQIRRLG
                     QKIGGLSATSLPRTIRMPEPFIHGYDITPGKTPEDISRFINPSGAWASGGIVSTLPNL
                     SRFFRAYVRGRFFGQGAGAGAIKRA"
BASE COUNT          291 a          559 c          563 g          282 t
ORIGIN      
        1 ttgaacgagc acgtggggcg gcgaatcgac atgccggtcg aggaaactct cgcctcgttc
       61 gcaggcaggg acgcgctcag gggccgaacg gtccgctggg cggagggtga gggcacggct
      121 tcggggatcg accgcgaggg gaatcttctg gtcgagtcga ccgcgggttg tctttcgaca
      181 ctgaatgccg gtgaagtcca cctctctctc ccggtcgacg ccgcatgagc cccggtagcg
      241 attccccgac gctcgccccg gtcgagtcag acggcctgcc gatcgacttc acccgtcccg
      301 gggtgatctg gctggatgag ctaccggtgc cgatcgagcc aaccggaacc ggcaaggcgg
      361 tcgcgcgcct gcgggaggga cggctcgtcg tcgggggcgg cagccggacc caggtcctgg
      421 ctgcgatcga ccgttggtac cggcgggaag cccggaagcg catcgaacca ctggtcgtta
      481 tcgaaggcga gcggctgggt ctcgaggcga agaaggtcag gatcgggaac cagcgaaccc
      541 gctgggggtc ctgctcgacc agcgggacag tttccttcaa ctggcgcctg gtgatcggcc
      601 ggccggaggc ccttcactac gtggtggtac acgaactgat ccacctcagg catcacaacc
      661 attcgcgggc attctgggac gcccttgccg aggcgttccc cgacttcaag cgggaggcga
      721 cctggctgcg gtccaacgag cgcgaactgc tcaaatggaa gccccgaatc tgatccggta
      781 gcgtggccgc atgatcgaag tcggcagggc aagcatccgg aagaccacga ccggagtgat
      841 tttcgtgctc tttgccctcg cgtcggcgtt tgcgatctcc catcccgccg aggcttcgac
      901 ccggtcgaac ctgaaccggg ccctgaacca ggtgatggaa gttccgaacg gaccgcccgg
      961 catctcgatg cagatcgtga agaacggcca ttcgcggtac ttccggcggg gcgtcgccaa
     1021 cgtgcggacc ggcgccaagc cccggctccg ggaacgtttc cggattgcca gcgtggccaa
     1081 ggccttcagt ggcgcggtcg ccctgaacct ggtcgcgcgg ggcaaactca gtctggattc
     1141 gacgatcggt gaagtcctgc ccggcatgat gccgaaggcc aggcaggtca cccttggcca
     1201 ggccctccag cacaccggcg gcctgccgga atacatcaag tcgaagggct tcatccggga
     1261 tatcaaccgg aacccggccg ccttcgtccc tccgacccgg atcatctcct gggtccgcaa
     1321 ctccgggctg acgcatcgtc cgggaacgaa gtacgagtat tccgacaccg acaacatcgt
     1381 ggtcggcctg atggcccagc gggtgaccgg cgttcgctac ctctggcaga tccgccggct
     1441 cggccagaag atcggcggcc tgtcggcaac ttcgctgccc cggacgatcc ggatgccgga
     1501 gcccttcatc cacggttacg acatcacgcc cggcaagacg cccgaggaca tctcccgctt
     1561 catcaatcct tcgggggcct gggcctcggg cggcatcgtt tcgaccctgc ccaacctgag
     1621 ccgcttcttc cgggcctatg tgagaggccg cttcttcggt cagggtgccg gggcaggggc
     1681 gatcaagcgg gcgca
//
LOCUS       DAOTIP010000515         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84434_length_1695_cov_3.753049, whole genome shotgun sequence.
ACCESSION   DAOTIP010000515 DAOTIP010000000
VERSION     DAOTIP010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84434_length_1695_cov_3.753049"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1471)
                     /locus_tag="P5138_09295"
     CDS             complement(125..1471)
                     /locus_tag="P5138_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRV60814.1"
                     /translation="MNHDTPERDPQGAAQLLRDESDAHVLKCMGDVDMGLVDGDGVHV
                     RDADGNEYLDAISAEWVLNLGFRHPEIKQAMEEQFGKIDYVSPVFEDEARTAYAKKLA
                     ELAPGALSKVLYGLSGASAVEGAMHLAMRTTGGTDFVCLDAAFHGRTFATIGLTYVNP
                     GMVEGSNKGLDRYLPRQMRVPNYYCYRCPLGLERETCDLACADQVEWALDKGHLNGPA
                     GVIVEPYQANGGMVPAPEGYLERVHEMAKDHDVPLIVDEIQGAFCRCGPMYASERAGI
                     EPEMIILGKAMGGGLPLSGTITTPDYAALLPWEYGFTQAGNPLACAAALAMVEVMVRD
                     DLPDNSERIGQLMTGELEKLMPDSKLIGDVRGQGLMIGVELVRDKETKEPAFEETDQL
                     MAACLQHGLMIGKTGPVFGSNGNVAKFKPAVNVTEEDAMEMIELFTRALRDVEATL"
     gene            complement(1492..>1695)
                     /locus_tag="P5138_09300"
     CDS             complement(1492..>1695)
                     /locus_tag="P5138_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60815.1"
                     /translation="VDFCAIAKAFNCGSEKVTEVGEIGASFERAMASDGPCLIEFDLS
                     SEPADSEGVNIGHWDLPKPAYLP"
BASE COUNT          279 a          562 c          520 g          334 t
ORIGIN      
        1 agttcgtcga cgtgggccat cgtccactcg accgagtcgt accccgcttc gagaagcgag
       61 tccagaacct cccgtgcctg cagactctcg aaatcgagag ctgcgatgaa gctgatttcc
      121 ctcttcacag agtggcctcg acatcacgaa gagcccgcgt gaagagttcg atcatctcca
      181 tcgcgtcttc ctcggtcacg ttgaccgcgg gcttgaactt ggcgacgttt ccgttcgagc
      241 cgaagaccgg gccggtcttg ccgatcatca ggccgtgctg gaggcaggcg gccatcagct
      301 ggtcggtttc ttcgaaggcc ggctccttgg tttccttgtc acggaccagc tcgacgccga
      361 tcatcagccc ctggccgcga acgtcgccga tcagcttcga gtccggcatc agcttctcca
      421 gctcgcccgt catcagctga ccgatccgct ccgaattgtc tggcaggtcg tcccgaacca
      481 tcacttcgac catcgccagc gcggctgcgc aggccagggg gttcccggcc tgggtgaagc
      541 cgtattccca ggggagcagc gccgcgtaat caggcgtggt gatcgtgccc gagagcggca
      601 gcccgccgcc cattgccttg ccgaggatga tcatctccgg ctcgatgccg gcccgctcgc
      661 tcgcgtacat cggaccgcag cggcagaacg cgccctggat ctcgtcgact atcagtggca
      721 cgtcgtggtc cttggccatt tcgtggaccc tctccagata gccctcggga gcgggaacca
      781 tgcctccgtt ggcctggtac ggctcgacga tcactccggc aggaccgttc aggtggccct
      841 tgtcaagagc ccattcgacc tggtcggcgc aggctagatc gcaggtttca cgctcaaggc
      901 caaggggaca ccggtagcag tagtaattcg gcaccctcat ctgccgcggc aggtaacggt
      961 cgaggccctt gttcgagccc tccaccatcc ccgggttcac gtaggtcaac ccgatcgtgg
     1021 cgaaggtccg gccgtgaaat gcggcgtcga ggcagacgaa gtccgtcccg ccggtcgtcc
     1081 gcatcgccag atgcatcgct ccctcgaccg ccgacgcgcc cgagagtccg taaaggacct
     1141 tggaaagggc acccggggcg agttcggcaa gcttcttcgc gtaggcagtc cttgcttcgt
     1201 cctcgaatac cggcgagacg tagtcgatct tgccgaactg ctcctccatc gcctgcttga
     1261 tttcggggtg gcggaaaccg aggttgagca cccattccgc ggagatcgcg tcgaggtact
     1321 cgttgccgtc ggcgtcccgg acgtgaacgc catctccgtc gaccaggccc atgtccacgt
     1381 cgcccatgca tttgaggaca tgggcatcgg actcgtcgcg gagcagctgg gcggcccctt
     1441 ggggatctcg ttcgggggtg tcgtgattca cctgttgctc cttggtctcg ttcagggcag
     1501 gtacgcgggc ttcggcaggt cccagtggcc gatgttcacg ccttcgctgt cagccggttc
     1561 gctcgagagg tcgaactcga tcaggcaggg accatcggag gccatcgccc gctcgaagga
     1621 ggcaccgatc tcgccgacct cggtcacctt ctcggatccg cagttgaagg cctttgcgat
     1681 cgcgcagaag tcgac
//
LOCUS       DAOTIP010000516         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84587_length_1693_cov_2.890720, whole genome shotgun sequence.
ACCESSION   DAOTIP010000516 DAOTIP010000000
VERSION     DAOTIP010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84587_length_1693_cov_2.890720"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..751
                     /locus_tag="P5138_09305"
     CDS             68..751
                     /locus_tag="P5138_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60816.1"
                     /translation="MTATRQEAESAADLVPGSSFSARIYEPFLWLGEKSGFRRWRRKL
                     ISQVSGSVLELGAGTGLNLAHYPAGLERLVLVEPDVHKARLLAQKTPPEGVSPEIVRA
                     PGEMLPFEDDTFDTVVETLVLCTVADPEKTAAEIRRVLKPGGRFLFMEHVRSDKPRLG
                     RLQDRLENPWKKIADGCHCNRRTVPMLRGSGFEVEVTAEQDKFPMPPVARPIVSGIAR
                     PAPVAAGAS"
     gene            755..1600
                     /locus_tag="P5138_09310"
     CDS             755..1600
                     /locus_tag="P5138_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRV60817.1"
                     /translation="MPELPEVEITARLVGDAVAGAKVESVLAPGVNVMRSFKPSLDVL
                     NGDEILGMRRRGKLLIIDFASGLSLLTHLMSSGKLQLFEKRAGSRDRTSRVLIRLDEG
                     HELRIRETGTRQRTWAKLMPTGEIEEDEAVAKLGPDAWPGTVAPELVSDTNRPLYSLL
                     RDQQTLAGIGRTWADEILWTARLSPFKRADDMEAEEIYRLIAAIPDRLGGAISFYEEE
                     LELPLPDKLPRPLKIHGREGEICPRCGGTIHAVHYKDYILCYCPHGQTNGQSLKDRRM
                     SRLLK"
BASE COUNT          304 a          535 c          588 g          266 t
ORIGIN      
        1 ggggccgtac gatcatcccg gagtgcccgt ttgtccgggg ctggatgcgg gaacatccgg
       61 aggagtcatg accgcaacaa gacaggaagc cgaatccgca gcggacctgg tgccgggaag
      121 cagcttctcg gcccgcatct acgaaccgtt tctctggctg ggggagaaat ccggtttccg
      181 tcgctggcgg cggaagctga tctcccaggt atccggatcg gtgctcgagc tcggggccgg
      241 gacggggctc aaccttgccc attatccggc cgggctcgaa cggctggtgc tggtcgagcc
      301 cgacgtccac aaggcaaggc tgctggcgca gaagaccccg ccggagggcg tttcgccgga
      361 gatcgtccgg gctccggggg agatgctgcc cttcgaagac gacaccttcg acaccgtcgt
      421 cgagaccctc gtgctctgca ccgtcgctga tcccgagaag acggcggcgg agatcaggcg
      481 ggtgctcaag cccggcggca ggttcctctt catggaacac gtgcgatccg acaagccgag
      541 gttggggcgg cttcaggacc ggctcgagaa tccttggaag aagatcgccg acggctgcca
      601 ctgcaatcgc cgcaccgtgc cgatgctgag gggcagcgga ttcgaggtcg aggtgaccgc
      661 ggaacaggac aagttcccga tgccgcccgt cgcccggccg atcgtcagcg gcatcgcccg
      721 ccccgcgccc gtagccgccg gcgcttcctg acggatgccg gagctgccgg aagtcgagat
      781 cacggcccgg ctcgtcggag acgccgtcgc cggggcgaag gtcgagtccg tccttgctcc
      841 gggcgtcaac gtgatgaggt ccttcaagcc gtcgctggac gtcctgaacg gggacgagat
      901 tcttggcatg aggcgccggg gcaagctgct gatcatcgat tttgcatccg gcctgtcttt
      961 gctgacccac ctgatgtcaa gcgggaagct gcagctcttc gaaaaacggg ccggctccag
     1021 ggaccgcact tcccgggtgc tgatccggct ggacgagggc catgagctgc ggatccgcga
     1081 gaccggaacc aggcagcgga cctgggcgaa gctgatgccg accggcgaga tagaagagga
     1141 tgaggcggtc gcgaagctcg ggccggatgc gtggccggga acggttgcgc cggaactcgt
     1201 gtccgacacc aaccggccgc tctactcgct gctcagggac caacagaccc tggcggggat
     1261 cggccggacc tgggctgacg agattctctg gacggcgcgg ctgtcgccct tcaagcgggc
     1321 cgatgacatg gaagcggaag agatctaccg gcttatcgcc gcgatcccgg acaggctggg
     1381 cggagcgatc tcgttctacg aagaggaact cgagctgccg ttgccggaca agctgccgag
     1441 accgctcaag atccacggcc gcgaagggga gatttgcccc cgctgcggcg ggacgatcca
     1501 cgcggtccac tacaaggact acatcctctg ttactgcccc cacggacaga ccaacgggca
     1561 gtcgctcaag gatcgccgca tgagccggct gctcaagtga gcaccggccg gggcgccagt
     1621 ctcagccgtc gaagtcagag tccggttcct cggagctgag gatcttgtcg gcctccgagt
     1681 agacggagcc ggc
//
LOCUS       DAOTIP010000517         1692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84662_length_1692_cov_3.413561, whole genome shotgun sequence.
ACCESSION   DAOTIP010000517 DAOTIP010000000
VERSION     DAOTIP010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84662_length_1692_cov_3.413561"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1692
                     /locus_tag="P5138_09315"
     CDS             <1..>1692
                     /locus_tag="P5138_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRV60818.1"
                     /translation="NNSTPASAGRLYRADAGVELFLAESPDRAAEIARELALVNAERR
                     KIEQEVESLAKTELKSLGEPGPAVVVAGEGWHPGVVGIVASKLVKATGRPSVVISLDG
                     ETGRGSARSVPGLDLHAALGETSGLLETFGGHAAAAGLTIRTDRIDEFREALGRAVSA
                     RIGDEPAERTIEFDAVAGGSDLGMDMAEEMEKLQPFGSGNPSVRLLVPGARIENLNEM
                     GEGKHCRFSIRTGSHLAKGVAFGRTSFGLAEGERADVVAELGINHWNGSIEPQLRVTH
                     VAAVPVAETLPECSPEEWWERFDIALEELDEIDPRGAAGAGSAGLDLASGPEGLPGVA
                     IAELISSGERVAILTADARQRWMGLGGAGLSRFFPDPADGPPAAGSASGSASGSASGS
                     ASGSGDGVAGLWAGSPRSDVEPAGRAQVLLTDFATISGSPAAGPGANPSLGEFDRIAL
                     LDPPFTVKAIEAVSAPAIPVHLLAGPDEFEFAGRVAGHRLDLTAQLRGLYRGLRDGAA
                     GEGGELSGDPLREVLLSDGNSTRSPEDSAILLRVLLETGLARSEGLGSARRVGAV"
BASE COUNT          256 a          542 c          635 g          259 t
ORIGIN      
        1 ggaacaactc gacgcccgcg tcggccggga ggctctaccg ggccgacgcg ggcgtcgagt
       61 tgttcctggc cgagtcgccc gaccgtgccg ccgagatcgc ccgggaactc gccctggtga
      121 acgccgaacg ccggaagatt gagcaggaag tcgaaagcct cgcgaagacg gagctgaaat
      181 cgctggggga acctggtccc gcagtggtcg tcgcaggcga agggtggcat cccggggtgg
      241 tcgggatcgt tgcctccaaa ctggtcaagg caaccggccg gccatcggtg gtcatctcgc
      301 tggacggtga aaccggccgg ggctcggcga ggtcggtccc gggccttgac ctgcacgcgg
      361 ccctgggaga gacctccgga ctgctggaga ctttcggggg acacgcggcg gcggccggac
      421 tgaccatcag gaccgaccgg atcgacgagt tccgggaagc cctcggaagg gcggtgtcgg
      481 cccggatcgg cgacgagcct gccgagcgga caatcgagtt cgatgcggtg gccggcgggt
      541 cagatctcgg catggacatg gccgaggaga tggagaagct ccagccgttc ggcagcggaa
      601 acccgtcggt gagacttctc gtgcccggcg cccggatcga gaacctgaac gagatggggg
      661 aggggaaaca ctgtcgcttt tcgatccgga cgggctccca tctcgccaag ggcgtcgcct
      721 tcggacgaac ctccttcggg ctcgcggagg gcgagagggc cgacgtcgtt gccgaactgg
      781 ggatcaacca ttggaacggc tcgatcgagc cccagctcag ggtcacccat gtggccgccg
      841 tgccggttgc ggagactctg cccgaatgct cgccggagga atggtgggaa cggttcgaca
      901 tcgccctgga agagctggac gaaatcgacc cccggggagc ggccggcgcc ggctcggccg
      961 gactcgacct cgcctccggc cccgaggggt tgccgggggt ggccatcgcc gagctgatca
     1021 gctccggcga gcgggtcgcg atcctgaccg ctgacgcccg gcaacgctgg atgggacttg
     1081 gcggggcggg gctctccagg ttcttcccgg acccggccga cggcccgccc gccgcgggat
     1141 ccgcgtcggg atccgcgtcg ggttccgcat cgggatccgc gtcgggttcc ggagacgggg
     1201 tggcgggact atgggccggc agtccacgct cggacgtaga gccggcggga agggcccaag
     1261 tgctgctgac cgacttcgcc acgatttccg ggtcgccggc ggcaggcccg ggggcgaacc
     1321 cgtcgcttgg ggagttcgac cggatcgctc tgctggaccc gcccttcacg gtgaaggcca
     1381 tcgaggctgt ttccgccccg gccatcccgg ttcacctgct ggcgggaccc gacgagttcg
     1441 agttcgccgg gagggtcgca ggccaccgtc tcgatctgac cgcacagctg agaggtctct
     1501 accgtgggct tcgtgacggt gccgcgggcg aaggtggcga gctctccggg gatcccctgc
     1561 gcgaggttct gctgtccgac ggcaattcga cccgctcgcc cgaggattcc gcgatcctgt
     1621 tgagggttct gctcgagaca ggtctggccc ggagtgaagg cctcgggagt gcccgtcgcg
     1681 tcggggccgt at
//
LOCUS       DAOTIP010000518         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84730_length_1691_cov_3.111858, whole genome shotgun sequence.
ACCESSION   DAOTIP010000518 DAOTIP010000000
VERSION     DAOTIP010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84730_length_1691_cov_3.111858"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5138_09320"
     CDS             <1..226
                     /locus_tag="P5138_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60819.1"
                     /translation="PYSRAEWHWRVPVAHNFGKAAAPQTVLGVLRRLDNEGFAGELVL
                     REVREGRSEVVQMWGRPESVREEFRQRRSI"
     gene            234..554
                     /locus_tag="P5138_09325"
     CDS             234..554
                     /locus_tag="P5138_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60820.1"
                     /translation="MARVLAVVPDLMLSSRVGAMLGAAGHEVKVLPAPPSGEEFDFDL
                     VVCDLEAVEAESIAALPAPAIGFYSHVDVETRNRGREAGLELVVPRSRMARELPDLAD
                     RLLG"
     gene            735..1484
                     /locus_tag="P5138_09330"
     CDS             735..1484
                     /locus_tag="P5138_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRV60821.1"
                     /translation="MQDRGLAAYIAELLGTFFLVFGITLVGVLFIAVGDNASFGSDFA
                     VVGLTYAFILTALIFAFGGASGGHFNPAITLGAAMMKKIAPVDAVIYVLAQLSGGVLG
                     ALLTKGLLEDEGRAANYGAVSVSPLLSGNFAGAVVECLGTFLLVLTVLAVALGIRAVK
                     DWAPLAIGLALGVVGLILVPLTGGAVNPARWFGPALVSGEWGGVWPYLGGEIVGALLA
                     YVVYKFVIAPGAGGSPEEEAKKPSTMPSPEA"
BASE COUNT          253 a          546 c          566 g          326 t
ORIGIN      
        1 gccctattcg cgagccgagt ggcactggcg cgtgccggtc gcccacaact tcggcaaggc
       61 tgccgcgcca cagacagtgc tcggtgttct ccgccggctc gacaacgaag gctttgccgg
      121 cgagttggtc ctgcgcgaag tccgggaagg gcgctccgaa gtggtccaga tgtggggccg
      181 tccggaaagc gtgcgcgaag agttccgcca gcgccggtcg atctgagccg gtcgtggcca
      241 gggtcctcgc ggttgttccc gacctgatgc tgtcgagccg ggtcggcgca atgctgggcg
      301 cggcgggcca cgaagtgaag gttctgccgg cgccaccgtc aggggaagag ttcgatttcg
      361 acctggtcgt ctgcgatctg gaagcggtcg aggcggaatc gatcgccgcc ctgcccgctc
      421 cggcgatcgg cttctactcc catgtcgatg tcgaaacccg gaaccgggga cgggaagccg
      481 gactggaact ggtcgttccc cgctcgcgga tggcccgtga gctgccggac ctggccgacc
      541 gcctgctcgg ctgaaagcgc ccatccaatc cggctgggac gggatccggg caatcatcgg
      601 cgtatatttc aaggcccaca caagcgaccc cgtattgcgc ttcacaactg tcggctccgg
      661 gctctggtta gactgccacc gctcgccgct aacggtgggg cgcaatcgat acaagccacg
      721 ggaggtttga cttcatgcaa gatcgcggtc tcgccgcata catcgccgaa ctgctcggaa
      781 cgttcttcct ggtgttcgga atcactctgg tcggcgtgct tttcattgcc gtgggcgaca
      841 acgcctcctt cggctctgac ttcgcggtcg tcggtctgac ctatgccttc atcctcacgg
      901 cactgatctt cgccttcggc ggagccagtg gtggccactt caacccggcg atcacgctcg
      961 gcgcagcgat gatgaagaag atcgctccgg ttgacgcagt gatctacgtg ctggcccagc
     1021 tctcgggtgg cgttctcggc gccctgctga ccaagggcct gctcgaggac gaaggtcgcg
     1081 ccgccaacta tggcgccgta tcggtcagcc ccctgttgag cggcaacttt gccggcgcag
     1141 tagtcgagtg cctcggtacc ttcctgctgg ttctcaccgt tctggcagtc gccctcggta
     1201 tccgggccgt caaggactgg gcgccgctgg cgatcggtct cgcgcttggt gtcgtcggtc
     1261 tgatcctggt cccgctgacc ggtggtgccg tcaacccggc ccgctggttc ggtccggccc
     1321 tggtctcggg cgagtggggc ggtgtgtggc cctacctcgg cggtgagatc gtcggcgctc
     1381 ttctggccta cgtggtctac aagttcgtga tcgcgcccgg agcgggtgga tcgccggagg
     1441 aagaagcaaa gaagccttcg acgatgcctt cgccggaggc ctgagccccg cgctcaacga
     1501 atctccgaaa agggcggccc tcgggccgcc ctcttctttg ggccggcccg gcctgcctta
     1561 tccggctatt acctggccca gggtcaccgc ggcgagtccg cagaccaggc tcagggacag
     1621 attgagccag gcgagcttcg ccaggtcagc catctggagc aggcgggtgt cgaggatcca
     1681 ggtcgagaag g
//
LOCUS       DAOTIP010000519         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84854_length_1689_cov_4.119339, whole genome shotgun sequence.
ACCESSION   DAOTIP010000519 DAOTIP010000000
VERSION     DAOTIP010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84854_length_1689_cov_4.119339"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..487)
                     /locus_tag="P5138_09335"
     CDS             complement(95..487)
                     /locus_tag="P5138_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60822.1"
                     /translation="MEFDQYHEPPEELSPELRTKARLFASLAEEAEAINWYEQRLSVE
                     PDAEARSIMHNAQHEEFKHFAMDLEFLFRRMPVWRQLAEGVLFRDGDIVEHGEAAEAG
                     TEEEDEGPSTGGGSDGSLGIGSLRGAGL"
     gene            complement(599..1162)
                     /gene="pyrE"
                     /locus_tag="P5138_09340"
     CDS             complement(599..1162)
                     /gene="pyrE"
                     /locus_tag="P5138_09340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRV60823.1"
                     /translation="MTDREATRQKLIGLLREHSLVLGEVTLSSGAQASYYIDARRTVM
                     RPEGLKAVGTLIALHAKELGATTVGGPVMAAIPLACAAIAVPEGEDLGGFFVRKERKA
                     HGLQRWVEGAAEPGDRVLVVEDTVTTGGSTISAIERIRDEGFEIAGVLAVVDRLAGGG
                     DKIAAEAGAPYETLTTIDDIYPDRPDK"
     gene            complement(1199..>1689)
                     /locus_tag="P5138_09345"
     CDS             complement(1199..>1689)
                     /locus_tag="P5138_09345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRV60824.1"
                     /translation="LFAREFPGGVNWRQAAGRLGFHRFRPGNAAKWIGIGIVAYIAFA
                     IIYVSIFGEPKQDDIAGDFGPVWSQILLIVILAPLSEEICFRGMLFGGFRTRFNLPVA
                     AIGAGLVFGALHFSTGPSAVPQLMVLGAIFAVVYDRTESIWPPIIFHAINNAYVLIVL
                     NS"
BASE COUNT          276 a          566 c          535 g          312 t
ORIGIN      
        1 ccggcgagcc gcgggaccag gctgtcgctg gcctcctcgt cgatctgctt ccaggcttcg
       61 tcggtgattg gggcatggcc ccgcaaaagg tgattcacag tcccgctcct ctcaggctgc
      121 cgattccaag cgaaccgtcg gaaccgcccc cggtcgaagg tccctcgtct tcctcttcgg
      181 ttcccgcctc ggctgcctcg ccgtgttcaa cgatgtctcc gtccctgaac aggacgccct
      241 cggccagctg gcgccagacc ggcatccgcc ggaagaggaa ttcgaggtcc atcgcgaagt
      301 gcttgaactc ctcgtgctgg gcgttgtgca tgatcgacct ggcttcggca tcgggctcaa
      361 ccgagagccg ctgctcgtac cagttgatcg cctcggcttc ctcggccagt gacgcgaaga
      421 gtctcgcctt ggtcctgagc tccgggctca gctcctccgg cggctcgtgg tactgatcga
      481 attccaatcc gggctccttt caggtcgtca gaccggtaac cggtgaattg gtacccatgc
      541 tttcactccg gcaaccccgg cacaaccccg ggcgatcccg gggtatccgg catggtctct
      601 atttgtcggg ccggtccggg tagatgtcgt cgatagtggt cagggtttcg tagggggctc
      661 ccgcctcggc ggcaatcttg tcgccgccgc cggcgagccg gtcgaccacc gccagaaccc
      721 cggcgatctc gaatccctcg tcgcggattc gctcgatcgc cgaaatcgtc gagccgccgg
      781 tggtgaccgt gtcctcgacg accagcaccc ggtcgccggg ttcggccgcc ccttcgaccc
      841 accgctgcag accgtgcgcc ttgcgttcct tgcgcacgaa gaaaccgccc agatcttcgc
      901 cttcgggaac cgcgatggcc gcgcaggcca ggggaatcgc cgccatcacc gggccgccca
      961 ccgtggttgc tcccagctcc ttcgcgtgga gggcgatcag ggtgccgacc gccttcaggc
     1021 cttccggacg catcacggtc cggcgcgcat cgatgtagta gctcgcctgg gcgccgctgg
     1081 agagggtgac ttcgcccagc accagggaat gctcacgcag gagtccgatc agcttctggc
     1141 gggttgcttc gcggtcggtc aaagggtcac ctttcggtcg gttcgcggtc ggtcaggttc
     1201 atgaattgag cacgatcagg acgtaggcgt tgttgatcgc gtgaaagatg atcggcggcc
     1261 agatcgactc ggtcctgtcg tagacgaccg cgaagatcgc cccgagcacc atcaactggg
     1321 gaacggcact gggtccggtt gaaaagtgga gggcgccgaa gacgaggccg gcaccgatcg
     1381 cggcgaccgg caggttgaac ctggtccgga agccgccgaa cagcatgccg cggaagcaga
     1441 tctcttcgct cagcggagcc aggatcacga tcagcaggat ctgcgaccag accggaccga
     1501 agtcgccggc gatgtcgtcc tgttttggct ccccgaaaat cgacacgtag atgatggcga
     1561 aggcgatgta ggcgacgatg ccgatgccga tccatttggc cgcattgccc ggcctgaacc
     1621 gatggaagcc gagcctcccg gcggcctgcc tccagttgac cccgcccggg aattccctcg
     1681 cgaagagga
//
LOCUS       DAOTIP010000520         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84940_length_1688_cov_3.856093, whole genome shotgun sequence.
ACCESSION   DAOTIP010000520 DAOTIP010000000
VERSION     DAOTIP010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84940_length_1688_cov_3.856093"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1688
                     /locus_tag="P5138_09350"
     CDS             21..>1688
                     /locus_tag="P5138_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRV60825.1"
                     /translation="MSAETPARNLKEAEAELRKLADAGYRTVVAFDSRGEAERARYGF
                     DRLDTKIVDSGAISAEPGVSLVEARLAEGFVSPRTRIAVLPFRRLLNRRRRADERAPE
                     AVRGRLTFSELRVGDHVVHEDHGVARFAGFETREVGGITRDYLYLEYKGEDRVYVPTD
                     QFAKLSRYSGGAGAPTLSALGGKKWLNMKARARGAASEIAGELVNLYAERKTRKGHAF
                     SPDSEWQMELEANFPWRETADQIEAIEAVKADMESEQPMDRLVCGDVGYGKTEVAIRA
                     AVKAASDGKQVMILAPTTILAQQHLGTFRERLADLPFRVDGVSRLRKPAEVKQVVADW
                     AEGKVDVLIGTHRLLSRDVRAKDLGLIVVDEEQRFGVKQKELLRQMKLKVDVLSMSAT
                     PIPRTLQMSMSGLRDISVIETPPEGRRPVRTYVGPWDEQLVERAIRREKDRKGQVFFL
                     HNRVESLDEAAESLRALVPGLRVQVAHGQMEDEELEAAMLNFLRGDADCLVATTIIES
                     GIDIPAANTLIVDRADHLGLAQAYQIRGRVGRSRERAFAYLLYPSEES"
BASE COUNT          289 a          559 c          582 g          258 t
ORIGIN      
        1 gggaggaatc gctccgggcg atgagcgccg agacgccggc ccgcaacctg aaggaggccg
       61 aggccgagct tcgaaagctg gccgatgccg gataccgcac cgtggtcgcc ttcgattccc
      121 gcggcgaagc agagcgggcc cgctacggat tcgatcgact tgacacgaag atcgtcgatt
      181 ccggggcgat atcggccgaa cccggggtca gcctggtcga agcccggctg gccgaaggtt
      241 tcgtctcgcc ccggacccga attgccgtgc tgcccttccg gcgtctgctc aatcgccgcc
      301 gccgggccga cgagcgggcc ccggaagcgg tccgtggccg gctgaccttc tccgaactgc
      361 gggtcggcga ccacgtggtc catgaggatc acggcgtcgc ccgttttgcc ggtttcgaga
      421 cccgcgaggt cggcgggatc acccgtgact acctgtacct cgagtacaag ggcgaggacc
      481 gggtctacgt gccgaccgac cagttcgcca agctctcccg ctattccggg ggcgccggcg
      541 ccccgaccct ttcggcgctg ggcggcaaga agtggctgaa catgaaggcc cgcgcccgag
      601 gtgccgcctc ggagatcgcc ggcgagctgg tcaacctcta cgccgaacga aagacccgca
      661 agggtcacgc cttcagtccc gactcggaat ggcagatgga gcttgaggcc aacttcccct
      721 ggcgcgagac cgccgatcag atcgaggcga tcgaagcggt caaggccgac atggagtcgg
      781 agcagccgat ggaccgcctg gtctgtggag acgtcggcta cggcaagacc gaggtcgcga
      841 tccgggctgc ggtcaaggcc gcctccgatg gcaagcaggt gatgatcctc gctccgacca
      901 cgatcctcgc ccagcagcat ctcggcacct tccgcgagcg cctcgccgac ctgcccttcc
      961 gggtcgacgg ggtcagccgc ctgcgcaagc cggccgaggt gaaacaggtt gtggccgact
     1021 gggcggaggg caaggtggac gtcctgatcg gcacccaccg gctgctctcc cgggatgtgc
     1081 gggcaaagga ccttggcctg atcgtggtcg acgaggagca acgcttcggg gtcaagcaga
     1141 aggagctgct gcgccagatg aagctgaagg tcgatgtgct ctcgatgtcg gccacgccga
     1201 tcccgcgcac cctccagatg tcgatgtccg gtctcaggga catctcggtg atcgaaaccc
     1261 cgcccgaggg caggcgcccg gtacggacct atgtcggtcc gtgggacgag caactggtgg
     1321 aacgggcgat ccgtcgggag aaggaccgca agggccaggt cttcttcctc cacaaccggg
     1381 tcgagtccct ggacgaagcc gcggagagcc tgcgggccct ggttccgggg ctccgggtcc
     1441 aggtggcgca cggccagatg gaggacgagg aactggaagc ggcgatgctg aacttccttc
     1501 gcggtgatgc cgactgcctg gtcgccacaa cgatcatcga gtcggggatc gacatccccg
     1561 cagccaacac cctgatcgtc gaccgggccg accatctcgg cctggcccag gcgtaccaga
     1621 tcaggggtcg cgtcggccgg tcccgggagc gggcttttgc ctacctgctc tacccctcgg
     1681 aggagtcg
//
LOCUS       DAOTIP010000521         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_84962_length_1688_cov_2.720759, whole genome shotgun sequence.
ACCESSION   DAOTIP010000521 DAOTIP010000000
VERSION     DAOTIP010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84962_length_1688_cov_2.720759"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1615)
                     /locus_tag="P5138_09355"
     CDS             complement(308..1615)
                     /locus_tag="P5138_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRV60826.1"
                     /translation="MAAKLEKTRHPGIYRRGARYVVRYTDGDGKTRQESARTLDDART
                     LKARRIAEVDAGEHQPASRIKFSEYATDWVGRYQGRGSRGFRESTRDDYRRDLNRYVI
                     PYFNGKVRRTVEQISPTDVAGFVAWLCDQDAQGKRVADEKRQKSAERDGVDAKTVKVK
                     TEPVYLADATVRRILSPLRACLSTALAEGLIRHNPTTGIALPARDEQRAIDSGSDHDQ
                     DEVKALTTDQLETFLRVAPVRWRTFFECLAVSGIRVSEAFALRWRDLELDGSNPNLKV
                     RRAYVRGRFGPPKSKYGKRTIPLSPSMVDRFRQRRKETEWPGDNDLVFPAENGQPHHQ
                     ENVRRRALKPTAEEAGVPWAGFHTFRHSCATRLFAEGRNAVQVQRWLGHHSPAFTLQV
                     YVHLMDDDLGGALEIAKEPRDSQSECSPSIRAELPNQRLGFVR"
BASE COUNT          300 a          521 c          524 g          343 t
ORIGIN      
        1 atttcgggct gtttccgtgg gtcaacgtga caccctcgtg acaccctcga ccgttggtac
       61 gctcccccga tggggagaaa gagtagagcc ttagtaccgg ccatcgttgt gtcttgctcg
      121 cttgccattg cgtcctgcgg cggagatgac aaagcgcagg taccgaacgt aatcgggctt
      181 tcggagggga aggccaaacg agccttggag gacgacggtt acaaagtccg gtcaatgggg
      241 ggaggaatag acctgagctt gaccatgggg gacgcaacgg acaatttttg cgctgccggt
      301 aagcaggtca tcgaacaaag cccagacgct ggtttggaag ctccgcccgg atcgacggtg
      361 agcattcaga ttgactgtcc ctaggctctt tcgcgatttc gagcgctccg ccaagatcgt
      421 cgtccatcag gtgcacatac acctggagag tgaacgcggg agagtggtga ccaagccacc
      481 tttgaacttg caccgcgttc ctaccttctg cgaatagccg ggtagcgcaa gagtgccgga
      541 acgtgtgaaa cccggcccac ggaacaccgg cctcttcagc ggtcggcttc aaagcccgtc
      601 gccggacgtt ctcctgatga tgtggctggc cgttctcggc aggaaaaact agatcgttgt
      661 caccgggcca ttcagtttcc ttgcggcgct ggcggaaccg atcgaccatt gaaggtgaca
      721 acgggatcgt cctcttgccg tatttcgact tcggcggtcc gaaccggccc cggacatagg
      781 cgcggcgcac tttcaggttc gggttcgacc cgtccagctc gaggtcgcgc caccgaagcg
      841 cgaaagcctc cgagacccgg atgccggaaa cggccaggca ctcgaagaac gttctccagc
      901 gcacaggagc caccctgagg aacgtttcta gctggtccgt agtgagggcc ttcacttcgt
      961 cctgatcgtg gtcagagccg ctgtcaatgg ctcgctgctc gtcgcgggcc ggtagggcta
     1021 tgcccgttgt cgggttgtgg cggatcaagc cctcggccaa agcggtcgaa aggcaagccc
     1081 ggagagggga gaggatgcgg cgcacagtcg catcagccag atagaccggc tccgtcttga
     1141 ctttcacggt ctttgcgtca accccgtcgc gctctgccga cttctggcgc ttctcgtcgg
     1201 cgacccgctt gccctgggcg tcctggtcac aaagccaggc cacaaaaccg gctacatcgg
     1261 tcgggctgat ctgctcgacc gtgcggcgga ccttgccgtt gaagtagggg atcacgtacc
     1321 tgttcaggtc gcggcgatag tcgtcacggg tcgattcccg aaaccctcgg ctccctcggc
     1381 cctggtagcg gcctacccaa tcggttgcgt actcgctgaa cttgatccgg ctggccggct
     1441 gatgttctcc ggcgtcaacc tcggcgatcc tccgggcctt gagggtccgg gcgtcgtcaa
     1501 gggtgcgggc tgactcctgg cgggttttgc cgtcgccgtc cgtgtaccgg accacgtaac
     1561 gggcacctcg gcgatatatg ccgggatgcc gtgtcttttc gagcttcgcg gccatcagct
     1621 agtcctccgt ccctgtttcc aacagttcgc acacccgccg tgcgttgact gcggcaccct
     1681 ccgctagc
//
LOCUS       DAOTIP010000522         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_85032_length_1687_cov_2.424020, whole genome shotgun sequence.
ACCESSION   DAOTIP010000522 DAOTIP010000000
VERSION     DAOTIP010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85032_length_1687_cov_2.424020"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="P5138_09360"
     CDS             complement(<1..340)
                     /locus_tag="P5138_09360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60827.1"
                     /translation="MPTPDDSSYLDSVRGQVPLYDALQDAAVAAVPFGPARVLELGTG
                     TGETASRLLGRFPDARVTGLDSDADMLFKAREIVDEVSLARMEDPLPDGPWDLVLSVL
                     SVHRLTDEQKQ"
     gene            377..946
                     /locus_tag="P5138_09365"
     CDS             377..946
                     /locus_tag="P5138_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRV60828.1"
                     /translation="MLIEKVDLDALGLSYGDATRIKGEVAPERVSLGGQDYEPVPAEP
                     GFVLDVSKTSAGYALRLRFEVELAGPCFRCLEDAKAKIKVDAREVDQPVDPIPVQGSS
                     RFDEDDENEDESSAAELTSPYVEEGTLDLAGWTHDALILSLPEQILCQSDCLGLCPVC
                     GETLNGADPAAHDHGQNLDPRWAKLRDLQ"
     gene            960..1169
                     /gene="rpmF"
                     /locus_tag="P5138_09370"
     CDS             960..1169
                     /gene="rpmF"
                     /locus_tag="P5138_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRV60829.1"
                     /translation="MAVPKKRTSSTRRDKRRATHKAAKARVNHCPQCHHPRLPHRVCP
                     NCGQYKGREVIAPKEQPFGTVTPDA"
     gene            1179..>1687
                     /locus_tag="P5138_09375"
     CDS             1179..>1687
                     /locus_tag="P5138_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRV60830.1"
                     /translation="MDFSGTTVALDGLGVENGFDVLLEGIRLASADGIGLRVFGPASS
                     IDVSGLDRVEVIDTVDQITNDDDPVPSVRSREDASIVRAVADVAAGNSEAVVSLGSTG
                     ATMAAATFGLKRSRGVKRPALAAQVPLPGRTTLFLDVGANLDVRAQHLVQFAWLGASF
                     SKSVMGVEHP"
BASE COUNT          286 a          571 c          562 g          268 t
ORIGIN      
        1 tctgcttctg ctcgtcggtc agccggtgaa cggaaagcac cgagaggacc agatcccacg
       61 gcccgtccgg cagcggatcc tccatccggg ccagactgac ctcatcgacg atctcgcggg
      121 ccttgaaaag catgtccgcg tcggagtcca gcccggtgac ccgggcatcg ggaaagcgtc
      181 ccaacagccg ggaagcggtc tcgccggtgc cggtgccgag ctccagaacc cttgcgggcc
      241 cgaacggtac cgctgcgacc gccgcgtcct gaagggcgtc gtagagcggg acctggccgc
      301 gtaccgagtc gagatagctg gaatcgtccg gggtgggcat cgagcgcatc gtattcgtct
      361 gccatgctgg aacgggatgc tgatcgaaaa ggttgacctc gatgccctcg gcctgtcgta
      421 cggcgacgca acccggatca agggtgaagt cgccccggaa cgggtgtcgc tgggcggcca
      481 ggattacgag ccggtcccgg ccgaaccggg gttcgtgctc gatgtttcca agaccagcgc
      541 cggttacgca ctccggctgc gcttcgaggt ggagctggcc ggcccctgtt ttcgctgtct
      601 ggaggatgcg aaggcgaaga tcaaggtcga cgcccgcgaa gtggaccagc cggtcgatcc
      661 gatcccggtc caggggtcga gccgcttcga cgaggatgac gagaacgagg acgagtcgag
      721 cgcggccgaa ctgaccagtc cctatgtgga agaggggacc ctcgacctcg cagggtggac
      781 ccacgacgcc ctgattctct ccctgccgga gcagattctc tgccagtccg attgtctcgg
      841 cctctgcccg gtctgcgggg agaccctgaa cggggccgac ccggccgcgc atgaccacgg
      901 ccagaacctc gatccccgct gggcgaagct tcgcgatctc cagtagactg ccgcgtctta
      961 tggccgtacc gaagaaacga acctcaagta cccgccggga caagcggcgc gccacccaca
     1021 aggccgccaa ggcccgcgtc aaccactgcc cgcagtgcca ccatccgcgc ctcccgcacc
     1081 gggtgtgccc gaactgtggc cagtacaagg gccgcgaggt catcgctccc aaagagcagc
     1141 ccttcggcac cgtcactccc gacgcctgag cgtaggcatt ggacttcagc gggactacgg
     1201 tcgcgcttga cgggctcggc gtagagaacg gcttcgacgt cctgctcgag ggcatccggc
     1261 tggcttcggc cgacggcatc ggcctcaggg tcttcggccc ggcatccagc atcgacgttt
     1321 cgggcctgga ccgggtcgag gtgatcgaca cggtcgacca gatcaccaac gacgatgacc
     1381 cggtgccctc ggtccgctcg cgcgaggacg cctcgatcgt ccgggccgtg gccgatgttg
     1441 cggccggcaa ttccgaagcg gtggtcagcc tcggctccac cggggcgacc atggctgcgg
     1501 cgaccttcgg cctgaagcgg tcgcgcggag tgaagcggcc agccctggcg gcccaggtgc
     1561 cgctgcccgg ccggacgact ctctttctcg acgtcggggc aaaccttgac gtcagggccc
     1621 agcacctcgt ccagttcgca tggctgggtg cctccttctc gaaatcggtg atgggggtgg
     1681 aacatcc
//
LOCUS       DAOTIP010000523         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_85755_length_1677_cov_3.255240, whole genome shotgun sequence.
ACCESSION   DAOTIP010000523 DAOTIP010000000
VERSION     DAOTIP010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85755_length_1677_cov_3.255240"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..304)
                     /locus_tag="P5138_09380"
     tRNA            complement(216..304)
                     /locus_tag="P5138_09380"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Ser,seq:gga)
     gene            384..472
                     /locus_tag="P5138_09385"
     tRNA            384..472
                     /locus_tag="P5138_09385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Ser,seq:cga)
     gene            complement(595..963)
                     /locus_tag="P5138_09390"
     CDS             complement(595..963)
                     /locus_tag="P5138_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV60831.1"
                     /translation="MAVTGTDFVTIPIQDFERSKAFYRDTLGLEESKQWGDLPAQEYE
                     TGNLTIAIMDPTAFGQEFRPAGGSSVVLQVDDFEASKADLEGKGVEFVTDTIDSGICH
                     QAYFRDPDGNVLGIHHRYAH"
     gene            complement(966..>1677)
                     /locus_tag="P5138_09395"
     CDS             complement(966..>1677)
                     /locus_tag="P5138_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60832.1"
                     /translation="PGYGSRFYGVGNELKPALMVLLLAGLAAALTGKPKSRKNAAIVL
                     GFGLGLGIILGSGRLGAGVGAAIIVASATAVAALMMLPGRLTWKRGAILVAAPVIGLA
                     LLAVLDLTTAGGQGHYSSVLSGGESSFLDDVRRRTTLAWQQLWNGNMPVITLTCLLAA
                     AFAIRNREMFRPWAGPIWPAALVGGLAGGLIGSFTEDSGPLLIVVATITLTGVCAYLL
                     GRPDDSRIASGESPQRQE"
BASE COUNT          288 a          551 c          552 g          286 t
ORIGIN      
        1 tgtcttcggg gcgacggatc ccaaggctgg cgcggccggt tcgatcatgg acatcctcga
       61 cgatccggcc ctgaaccacc gccccgaagt ggagtctggc ctcctcgcac aggagtgcgg
      121 ggacctcctc aggaaattct tcgccagccg ccgctgacac ccggcaggca gaggccgtgg
      181 tcgtccggcc cggaccggac gacatacgga tgtaacggag agggtgggat tcgaacccac
      241 gaggcgagag atcccgccca cgcgatttcc aatcgcgctc cttaagccgc tcggacacct
      301 ctccggggac agggaagggt acccgagaac cgtggccgaa ggtgccgtga ccgggcccga
      361 atcgctagcc tgtcagtccc cccggagggg tgccagagcg gttgaatggg gcggtctcga
      421 aaaccgttgt gcgtcttacg gcgtaccgtg ggttcgaatc ccaccccctc cgtcatatgt
      481 cctcatgtcg tccggtcttc gccggacgac tcgggcatct gcaattcacc cacgctgttg
      541 cgggaaaacg acggttgcgg gcgggtttgt ccggttcggg ccggactcct gcggtcagtg
      601 ggcgtagcgg tggtgaatcc cgaggacgtt gccgtccgga tccctgaaat aggcctgatg
      661 gcagatcccg ctgtcgattg tgtccgtgac gaattcgacg cccttgccct cgaggtctgc
      721 tttcgatgcc tcgaagtcat cgacctgaag cacgacgctg ctgccaccgg cgggacggaa
      781 ttcctggccg aaggcggtcg ggtccatgat cgcaatggtc agattgccgg tctcgtattc
      841 ctgcgccgga agatcgcccc actgcttgct ctcctcgagg ccgagcgtgt cccggtagaa
      901 ggccttcgag cgctcgaagt cctgaatcgg gatggtgacg aagtcggtac ctgtaacagc
      961 cactttcact cctgtcgttg gggggactca cccgatgcta tccgcgagtc gtccgggcgt
     1021 ccaaggaggt aggcgcaaac gccggtcagg gtgatggtcg cgaccacgat cagcagcggg
     1081 cccgagtctt cggtgaacga tccgatcagt ccgcccgcca gaccgccgac cagggcggcc
     1141 ggccagatcg ggcctgccca cggcctgaac atctcccggt tgcggatcgc gaaggcagcg
     1201 gcaagcaggc aggtcagggt gatcaccggc atgttgccgt tccagagttg ctgccaggcc
     1261 aaggtggttc gccggcgaac gtcatcgagg aagctgctct cgcctcccga cagcaccgag
     1321 ctgtagtggc cctggccccc tgctgtcgtc aggtcgagga cggcgagcag cgccaggccg
     1381 atgaccgggg cggcgaccag gatcgcaccc cgcttccagg tgagcctgcc cggaagcatc
     1441 atcagggcgg ccaccgccgt cgccgaggcg acgatgatcg ccgcacccac tcccgcccca
     1501 aggcgtcccg agcccaggat gatccccagg ccgagcccga agccgagcac gatcgcggcg
     1561 ttcttcctcg atttcggctt gccggtcagg gcggcagcaa ggccggcgag caggagcacc
     1621 atgagtgcgg gcttgagttc gttccccacc ccgtagaagc gtgatccgta tcccgga
//
LOCUS       DAOTIP010000524         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_85763_length_1677_cov_2.895808, whole genome shotgun sequence.
ACCESSION   DAOTIP010000524 DAOTIP010000000
VERSION     DAOTIP010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85763_length_1677_cov_2.895808"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="P5138_09400"
     CDS             complement(<1..541)
                     /locus_tag="P5138_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRV60833.1"
                     /translation="MSENLRIGLLGRGTVGSAFDELVAERADQVEAATGRRPEVTGVL
                     TRSQGDFGEILPDADVIVELMGGTDTAREHVLAALRAGVPVVTANKQLLAQHGAELFA
                     AAEEGGVQLRFEAAVAAVVPVIRVVQESFGAVEFSKVFGIVNGTTNFILSEMAATGAS
                     YAEVLKRAQELGYAEADPTE"
     gene            complement(538..>1677)
                     /gene="lysA"
                     /locus_tag="P5138_09405"
     CDS             complement(538..>1677)
                     /gene="lysA"
                     /locus_tag="P5138_09405"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRV60834.1"
                     /translation="AEMRERARAYQRAFAAHTDHFEVLFASKALPVTAAYRLFAEEGL
                     SVDVASGGELTMALTAGFDPARIYMHGNNKTDAELVLAGNSGVGHLIVDSFDEIDRCE
                     RVLKRKQDVLIRVTPGIKPSTHSFIQTGQLDSKFGFGLEDGTAAKAITRVLASDTLNL
                     VGLHFHIGSQIFEIEPFRLAVEALGELRGDWCSILDLGGGLGISYDEADSPPPIEEYV
                     NLKVQAVRDVFGDEVKILIEPGRSLVGTAGVTIYRIGTVKEIPGVRTYVAVDGGMSDN
                     LRPMLYDAKYTAVIADRPDDPAGTTVTVAGSHCESGDILIRDVELANPRVGDILLTPA
                     TGAYGHSMSNNYNGMPRPPVIFCEDGEATVVVRRETWDDLLRRDM"
BASE COUNT          282 a          554 c          551 g          290 t
ORIGIN      
        1 cctcggtcgg gtcggcctcg gcgtaaccga gttcctgggc ccgcttcagc acctcggcgt
       61 aggaggcacc ggtggcggcc atctcggaaa ggatgaagtt cgtcgtcccg ttgacgatgc
      121 cgaagacctt gctgaactcg accgcgccga agctttcctg aaccacgcgg atcaccggca
      181 cgaccgcagc cactgcggcc tcgaagcgga gctggacccc gccttcttcg gcggcggcga
      241 agagctcggc cccgtgctgg gcgagcagtt gcttgttcgc ggtgaccacc ggcacgccgg
      301 ccctgagcgc cgccagcaca tgttcgcggg cggtgtcggt gccgcccatc aactcgacga
      361 tcacgtcggc atcgggcagg atctcgccga aatcaccctg gctgcgggtc aggaccccgg
      421 tcacctccgg ccgacggccg gtcgccgcct cgacctggtc ggcccgttcg gcaacaagtt
      481 catcgaaggc ggagccgacg gttccccgtc cgagcaggcc gatgcgcagg ttctcgctca
      541 catgtccctc ctgagaaggt catcccaggt ttcgcgtcgc accaccacgg tggcttcgcc
      601 gtcttcacag aaaatcacgg gcggccgcgg cattccgttg tagttgttcg acatcgagtg
      661 cccgtaggcg ccggtcgccg gggtaagcag gatgtccccc acccgggggt tcgcgagctc
      721 gacatcacgg atcaggatat ctccggactc gcaatgtgaa ccggcgacgg tgacggtggt
      781 cccggcgggg tcatcgggcc ggtcggcgat caccgccgtg tacttcgcgt catagagcat
      841 cggccggagg ttgtcggaca tgccgccgtc caccgcgaca taggtccgga ccccggggat
      901 ctccttcacg gtcccgatcc ggtagatcgt caccccggcg gtaccaacca gcgagcggcc
      961 cggctcgatc aggatcttga cctcgtctcc gaagacgtcg cgaaccgcct ggaccttgag
     1021 gttcacgtac tcctcgatcg gcgggggtga atcggcctcg tcgtaagaga tcccgaggcc
     1081 gccgccgagg tcgaggatcg agcaccagtc gccgcgcaac tcgccgagtg cctcgaccgc
     1141 caggcggaag ggctcgatct cgaagatctg ggagccgatg tgaaaatgaa ggccgaccag
     1201 attcagggtg tccgaggcaa gcacccgggt gatcgctttg gcggctgtac cgtcctccag
     1261 cccgaagccg aacttcgagt caagctggcc ggtctggatg aaggagtggg ttgacggttt
     1321 gatgcccggg gtgacccgga tcagcacgtc ctgcttgcgc ttcagcaccc gctcgcatcg
     1381 atcgatttcg tcaaacgagt cgacgatcag gtggccgacc cccgagttgc cggcgagcac
     1441 cagctcggcg tcggtcttgt tgttgccgtg catgtagatc ctggccgggt cgaaaccggc
     1501 ggtaagggcc atggtcaact cgccacccga ggcgacatcg accgaaagtc cttcttcggc
     1561 gaagagccgg taggcggccg tgaccggcag ggccttgctc gcgaaaagca cttcgaagtg
     1621 atcggtatgt gcggcgaagg cacgctgata ggcccgggcc cgttcccgca tttcggc
//
LOCUS       DAOTIP010000525         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_85827_length_1676_cov_2.884022, whole genome shotgun sequence.
ACCESSION   DAOTIP010000525 DAOTIP010000000
VERSION     DAOTIP010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85827_length_1676_cov_2.884022"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="P5138_09410"
     CDS             <1..776
                     /locus_tag="P5138_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60835.1"
                     /translation="GCLADETPDFAFRLSSGSYGCEAPQWRGQHRADGQVDFEHLDLL
                     AEGGGKVAGRLRDEVLEQAGLGEVAAARSGGANADWTGKKKRGLLGLVSRALRVKDDS
                     TAEFADARGFSPVDPSAFHMAHIELPLPAAADDIFEGSLADGRRVTLAWMEYENEIDG
                     LRYYVSLISEIRGPGSKNLSFWLDDQELLAAGSAPGVDPDLAQKARGAGVGLSAGGGA
                     ACVYLTSDGWSGRISGKEIERMLEAAPAIFNAADRQAGA"
     gene            835..>1676
                     /locus_tag="P5138_09415"
     CDS             835..>1676
                     /locus_tag="P5138_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRV60836.1"
                     /translation="MSLKLGLYLGYWGIGPRGKDAIEAVQFAESIGYESVWVAESYGS
                     DCVSVLAWLAAQTEKINLGAAIMQVPARPPAAAAMAGATIDYLSNGRFMFGFGPSGPQ
                     VSEGWYGVPYGKPWGRTKEYVEVVREIVAREEKLDHQGEHYQLPLPEGQGKPLKLNFK
                     PLRNDIPIFIGAIGRRSVEIAAEVADGWIPIFFSPEKFEEAWGEHIEKGLAAGGRTRE
                     DFEISPSVQVAIDGDLEAARNMVRMALVLYLGGMGSKKTNFYVDLSHRFGFGDVADKV
                     QELF"
BASE COUNT          281 a          536 c          576 g          283 t
ORIGIN      
        1 tggggtgcct cgcagacgaa actcccgatt tcgccttccg gctctcctcc gggagttacg
       61 gctgcgaggc accccagtgg aggggccagc accgggccga cgggcaggtc gatttcgagc
      121 accttgacct gctggccgaa ggtggcggca aggtggcggg acggctgcgc gacgaggtcc
      181 tggaacaggc cgggctcggg gaggtcgccg ccgcccgaag cggaggcgcc aacgccgact
      241 ggaccggcaa gaagaagcgc ggactgctgg gtctggtttc ccgggcgctc agggtcaagg
      301 acgattcgac ggccgaattc gccgacgccc gcggcttttc cccggtcgac ccttccgcct
      361 tccacatggc tcacatcgaa ctgcctctgc cggcggccgc cgatgacatc tttgagggca
      421 gccttgccga cggtcgccgg gttaccttgg cctggatgga atacgagaac gagatcgacg
      481 gtcttcgcta ttacgtctcg ctgatctcgg agattcgcgg tcccgggtcg aagaacctgt
      541 ccttctggct ggacgaccag gaactcctgg ctgccggttc ggcaccgggc gtcgatcccg
      601 acctcgccca gaaggcccgg ggcgccggtg tcgggctttc ggccgggggt ggagccgcct
      661 gcgtctacct cacctcggac ggctggagcg gccgcatctc cggcaaggaa atcgaacgca
      721 tgctcgaggc cgcgccggcg atcttcaacg ccgccgaccg gcaggccgga gcctgattcc
      781 ccggagcgag tacggaacaa atacgaaatt tgttctttcg gttaggatgg cggcatgtca
      841 ctgaagcttg ggctgtatct cgggtactgg ggcatcggac cccgcggcaa agatgcgatc
      901 gaggcggtgc agttcgccga gagcatcggt tacgaatcgg tctgggtggc cgagtcgtac
      961 ggatcggact gcgtcagcgt cctggcctgg ctggccgcgc agacggagaa gatcaacctc
     1021 ggcgccgcga tcatgcaggt gccggcccgt ccgccggctg ctgccgcgat ggccggtgcg
     1081 acgatcgact acctgtcgaa cggacgcttc atgttcggtt tcggcccgtc cggaccgcag
     1141 gtatccgagg gctggtacgg agtcccgtac ggcaagccct ggggccgcac caaggaatac
     1201 gtcgaagtgg tccgggagat cgtcgcccgc gaggagaagc tcgaccacca gggcgagcac
     1261 taccagctgc ccctcccgga gggccagggc aagcccttga agctcaactt caagccgctt
     1321 cgcaacgaca tcccgatctt catcggggcg atcggccgcc ggtcggtcga gatcgcggcc
     1381 gaggtcgcgg acggctggat cccgatcttc ttcagcccgg agaagttcga ggaggcctgg
     1441 ggcgagcaca tcgagaaggg gctcgccgcc ggcggtcgca cgcgggaaga cttcgagatc
     1501 tcgccatcgg tgcaggttgc gatcgacggt gacctcgagg ctgcccgcaa catggtccgc
     1561 atggcgctgg tcctctacct gggcggcatg ggctcgaaga agaccaactt ctacgtcgat
     1621 ctctcccatc gcttcgggtt cggcgacgtc gccgacaagg tccaggaact gttcca
//
LOCUS       DAOTIP010000526         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_85918_length_1675_cov_1.955556, whole genome shotgun sequence.
ACCESSION   DAOTIP010000526 DAOTIP010000000
VERSION     DAOTIP010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85918_length_1675_cov_1.955556"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5138_09420"
     CDS             <1..237
                     /locus_tag="P5138_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60837.1"
                     /translation="GDDGRDRLGLRVLETAPQAADQLEVINRPGETRRRRLGEAARQV
                     RAAVGESALMRILETEPESRLPERRMLLTPYSSE"
     gene            252..524
                     /locus_tag="P5138_09425"
     CDS             252..524
                     /locus_tag="P5138_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60838.1"
                     /translation="MRRATMVTSGTRPTRLYEPRPVDVASNAGNAPIQVNDVDVVQIR
                     EEWIVEDGWWTAAPVSRWYFEVVLIDGRDLTVFRAFPSGQWFAQRA"
     gene            531..>1675
                     /locus_tag="P5138_09430"
     CDS             531..>1675
                     /locus_tag="P5138_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV60839.1"
                     /translation="MGPYVELHCHSSFSFLDGASSPLELAEAAAELGYPALALTDHDG
                     IWGSMEFAVACKGAGIRAITGTELSVEDGGRIFHLTLLAENGAGYRNLCRLLTRAHAD
                     TRESRDRRAGQPRVPLTELADRAEGLIRLTGCARQGLIPAAWADGDLARGESLLRKLR
                     RWFSPENLRVEIQRPYWRHDHARNRWLAALARSLGLPLIATGNVHSHEPERARLQDAL
                     VAVRLGSNLADSEPHRRGNSTSALASPEQMAHRFPDYPEAVAETVRVAGRIGFDLERN
                     LGYRYPQQGDPRIDRDLAALCQSRMDERYRGTPQEQGARARLENELRIIHGLGLSGFF
                     LLHHDLLELAREVAAEVRGSSTARGVLPPGRGRGSSVSSIVCYLTGLS"
BASE COUNT          252 a          601 c          570 g          252 t
ORIGIN      
        1 ggcgacgatg gccgagaccg gctcggcctg cgtgtgctgg aaacggcacc acaggcggcc
       61 gaccagctgg aagtgatcaa tcggcccggc gaaacccggc gacgccggct cggcgaggca
      121 gcccgccagg tccgtgccgc ggtcggcgag agcgccctga tgcggatcct ggaaaccgag
      181 cccgagtcgc gcctgcctga acgacgcatg ctcctgaccc cttactccag cgagtagagg
      241 tgagcgcccc gatgagacga gcgaccatgg tcacttccgg caccaggccg acccggctct
      301 atgaaccccg ccctgtcgac gtggccagca acgccggcaa cgccccgatc caggtcaacg
      361 atgtggacgt ggtccagatc cgggaggaat ggatcgtcga ggacggctgg tggaccgccg
      421 ccccggtcag ccgctggtac ttcgaggtgg tgctgataga cggtcgcgac ctgaccgtct
      481 tccgcgcctt tccctccggg cagtggttcg cccagcgggc ctgaggggcc gtgggcccct
      541 acgtcgaact tcattgccat tcctcgttct cgtttctgga cggggcatcc tctccgctcg
      601 agttggccga ggcggcggcg gagctcggct acccggccct ggccctgacc gatcacgacg
      661 ggatctgggg gtcgatggag ttcgccgtcg cctgcaaggg cgcggggatc cgggcgatca
      721 ccgggacaga gctgagcgtc gaggatggcg gacgtatctt tcacctgacc ctgctggccg
      781 aaaacggggc cggataccgg aacctctgca ggctcctgac cagggcccat gccgataccc
      841 gggaaagccg ggaccgccgg gccggccagc cccgggtccc tctgacggaa ctggccgacc
      901 gggccgaggg gctgatccgc ctgaccggct gcgccaggca ggggctgatc ccggccgcct
      961 gggccgacgg cgacctcgcc cgcggggagt ccctcctccg gaagctccgg cgctggtttt
     1021 cccccgagaa cctgagggtc gagatccagc ggccctactg gcgccacgac catgcccgca
     1081 accgctggct ggccgcgctc gcccgcagcc tcggcctgcc gctcatcgcc actggcaacg
     1141 tgcattccca cgaaccggag cgggcccggc tccaggatgc cctggtcgcg gtccgcctcg
     1201 gctcgaacct ggccgactcc gaacctcaca ggcggggcaa ctcgacctcc gccctcgcct
     1261 cgccggagca gatggcccac cggtttccgg attacccgga ggccgtcgcc gaaaccgtcc
     1321 gggtcgccgg gagaatcggc ttcgacctcg aacgcaacct cggctaccgg tatccgcagc
     1381 agggtgaccc gagaatcgac cgggatctgg cggcactctg ccagtcccgc atggacgagc
     1441 gttaccgggg aaccccgcag gaacaggggg cccgggcgag gctggagaac gagctgcgga
     1501 tcatccacgg cctcgggctc tccggcttct ttctcctcca tcacgacctg ctggagctgg
     1561 cccgtgaggt agcggccgag gtgcggggtt catcgacggc ccggggcgtt ctgcctccgg
     1621 gccgtggccg cggctcttcg gtcagttcga tcgtctgtta cctgaccggc ctctc
//
LOCUS       DAOTIP010000527         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_86476_length_1667_cov_3.080025, whole genome shotgun sequence.
ACCESSION   DAOTIP010000527 DAOTIP010000000
VERSION     DAOTIP010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86476_length_1667_cov_3.080025"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="P5138_09435"
     CDS             <1..455
                     /locus_tag="P5138_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60840.1"
                     /translation="LFGGPGRDALAVGPMGRRFHRYRYKGRGLDMELFIYPDHKIEIS
                     LEYRIKCQRGGGYATGSGSLLGTTKVDQETGRFRFEDAYEVSDWYSWNERGKGRVTPE
                     AIYLLYSHYSVDRGDYFDPQICHTGTDKNSAIWIKAVRTPDPRQIARQ"
     gene            complement(493..>1667)
                     /locus_tag="P5138_09440"
     CDS             complement(493..>1667)
                     /locus_tag="P5138_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV60841.1"
                     /translation="GHDFETLDAGGGILCPPLVNGHTHAAMTLFRGHGDDLPLMRWLT
                     EAIWPVEAKLEDEDVYWGTRLACLEMIRSGTTGFWDMYWQPQATARAVEDAGIRTAIG
                     PPLFDAPDKTFEERNRELEGWLDELAAFGDRVTAVVAPHAIYTVGTASLEYAVGLAAE
                     RDLPIHIHLSETEGEVTDCVAAHGMRPAAYLDSLGMLTPKTTLAHGVWLDREELELIA
                     DRGATVTTNPVANMKLAVGGVFPYPEASRAGVPMALGTDGAGSNNSLDLLADLKVFAL
                     AQRHNSVDAEVIPIEEAWAIATGQKSELIRSLGRPALPAADPLTVGAPADFVVLDPKA
                     LELSVGNLTSALVYAASGSIVKDTVVDGRILMRNREVEGHEEILTRALERAARLFA"
BASE COUNT          300 a          514 c          557 g          296 t
ORIGIN      
        1 cactcttcgg cggtcccgga cgagatgcat tggcggttgg accgatggga cgccgattcc
       61 accgataccg ctacaagggc cgaggcctgg acatggaact tttcatctac ccggaccaca
      121 agattgagat cagcctggaa taccgcatca agtgtcagcg gggaggcggg tacgcaacgg
      181 gttccggttc gctgctaggt accacaaagg ttgatcagga gactggcaga ttcagatttg
      241 aagacgccta cgaagtcagc gactggtaca gctggaacga gaggggcaaa ggccgggtta
      301 ctcccgaagc catctacctg ctgtactcgc actactcagt ggacagagga gattacttcg
      361 atccccagat ttgccacaca ggaacggaca agaacagcgc gatctggatc aaagcagtga
      421 ggacaccaga ccccagacaa atcgcccgtc aatagcgcac tagcgattcg cataggaccc
      481 gctgggctac ctctaagcga agaggcgggc ggcgcgttcg agggccctgg tgaggatttc
      541 ctcgtggcct tctacttcgc ggtttctcat caggatccgt ccgtccacca cagtgtcttt
      601 gacgatcgat ccgctggccg cgtagacgag ggctgaagtc aggttgccga ccgagagttc
      661 gagtgccttt gggtcgagga cgacgaagtc ggcgggagct ccgaccgtca gcggatcggc
      721 cgctggcagg gcggggcgtc cgagactgcg gatcagttct gacttctggc cggtggcgat
      781 tgcccaggct tcctcgatcg ggatcacttc ggcatcgacc gagttgtggc gctgggcgag
      841 cgcgaagacc ttgaggtcgg ccagcaggtc gagcgagttg ttcgaaccgg ctccgtcggt
      901 gccgagcgcc atcggcactc cggcccggga cgcctcggga tagggaaaga ccccgcccac
      961 ggcgagcttc atgttggcca ccggattggt cgtgaccgtg gcgccccggt cggcgatcag
     1021 ctccagttcc tcccggtcca gccagacccc gtgggccagc gtcgtcttcg gagtcagcat
     1081 gcccaggctg tcgagatagg cggccggtcg catgccgtgc gctgcgacgc aatcggtcac
     1141 ctcgccttcg gtctccgaga ggtggatgtg gatcggcagg tcccgttccg cggccaggcc
     1201 gaccgcgtac tcgaggctgg ccgtgccgac cgtatagatg gcatggggag cgactaccgc
     1261 ggtgacccgg tcgccgaagg cggccagctc gtccagccag ccttcgagtt cccggtttcg
     1321 ctcctcgaag gtcttgtccg gcgcgtcgaa gagcggcggg ccgatcgccg tgcggatgcc
     1381 tgcgtcctcg accgccctgg cggttgcctg cggctgccag tacatgtccc agaatccggt
     1441 cgtgccggaa cggatcatct ccaggcaggc gagccgggtg ccccagtaga cgtcctcatc
     1501 ctccagcttc gcctcgaccg gccagatcgc ctcggtcagc cagcgcatca ggggaaggtc
     1561 gtcaccgtgt ccgcggaaga gggtcatcgc agcgtgggtg tgaccgttga ccaggggcgg
     1621 gcagaggatg ccgccgccgg cgtcgagggt ttcgaagtcg tgaccac
//
LOCUS       DAOTIP010000528         1666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_86557_length_1666_cov_2.696462, whole genome shotgun sequence.
ACCESSION   DAOTIP010000528 DAOTIP010000000
VERSION     DAOTIP010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86557_length_1666_cov_2.696462"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="P5138_09445"
     CDS             <1..594
                     /locus_tag="P5138_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60842.1"
                     /translation="VASFEFDGEMPEMIVRSLNGLTPPQIAVRAVTPVSGFDARRDAV
                     SRTYCYRVLTRRPGSPFAANRAWWVSRAIEREALDCCAAALVGQHDFTAFTPTETYHK
                     RFERIIHSAEWREEEGFLGGDILQFRVTGDSFMRNMVRILVGTMIEVADGSRSLANFE
                     SLLKGAQRPDAGITAPPQGLYFVSVDYPDPPPRPVAG"
     gene            626..1435
                     /gene="surE"
                     /locus_tag="P5138_09450"
     CDS             626..1435
                     /gene="surE"
                     /locus_tag="P5138_09450"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRV60843.1"
                     /translation="MRILLTNDDGIGAKGLQSLRRELRQIEGVTLDVIAPDSNRSASA
                     RSITTRAPLSVEEIRFEDGDLGHATDGTPVDCVRFAQVGLLGETPDLVVSGINHGLNL
                     GDDVTYSGTVAAALEGILLGLPAIAVSQQSEKGEMGYMPQRHYEFETVAKFTAGLVRE
                     LAGHPLPADTLLNINAPGGKPSGVDVTKLGKRLYRDELKKVERDVSDGRTSYRIYGYE
                     PGLDDEPGTDIGAIARGRLTVTPLSLDWTHHEELEGLRDRDFERLLGVASS"
     gene            1432..>1666
                     /locus_tag="P5138_09455"
     CDS             1432..>1666
                     /locus_tag="P5138_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60844.1"
                     /translation="MTDGASDGSDQERISELRDILNHHIVLYYEKDDPEISDEEYDEL
                     FTELQRLEKANPELITGDSPTQRVGAPASTSFAP"
BASE COUNT          300 a          551 c          545 g          270 t
ORIGIN      
        1 gtcgccagct tcgaattcga cggcgagatg cccgagatga tcgtccgcag cctgaacggc
       61 ctgacaccgc cacagatagc ggtgcgggcg gtgactccgg tctccggctt cgacgcccgc
      121 cgagacgcag tttcccgcac ctactgctac cgggtgctga cccgccggcc gggcagtccc
      181 tttgccgcga accgcgcctg gtgggtttcg cgtgcgatcg aacgcgaggc tctggactgc
      241 tgcgctgccg ccctggtcgg ccagcatgac ttcacggctt tcacaccgac cgagacctac
      301 cacaagcgat tcgagcgaat cattcattcc gccgagtgga gggaggagga aggattcctg
      361 ggtggcgaca tcctccagtt ccgggttaca ggtgactcct tcatgcggaa catggtccgg
      421 atcctggtcg ggacgatgat cgaggtcgcc gacggcagcc gcagcctggc gaacttcgag
      481 tcactcctga aaggtgctca gcggccggat gccggtatca cggcaccgcc gcaggggctc
      541 tatttcgtga gcgtcgacta cccggatcct ccccccagac ccgtcgcggg ttgagcgtta
      601 ccgcggttac atatccttgg agccgatgag gattctgctt accaatgacg acgggatcgg
      661 ggccaagggg ctgcagtcgc tgcggcgcga gctgcggcag atcgagggag tgaccctcga
      721 cgtgatcgcc ccggactcga accgcagcgc atcggccagg tcaatcacca ccagggcacc
      781 gctctcggtc gaggagatcc gcttcgagga cggcgacctc ggacatgcca ccgacggcac
      841 gccggtcgat tgtgtccgct tcgcccaggt cggcctgctc ggcgagacgc cggacctggt
      901 cgtctcggga atcaaccacg gtctcaacct gggagacgac gtcacctatt cgggcacggt
      961 cgcggctgcc ctcgaaggga tcctgctcgg cctgccggcg atcgccgttt cgcagcagtc
     1021 ggagaagggg gagatggggt acatgcccca gcgtcactac gagttcgaga cggtcgcgaa
     1081 gttcactgcc ggcctggtcc gggagttggc cggtcacccc ctgccggcgg acaccctgct
     1141 caacatcaac gcgcccggtg gcaaaccgtc cggggtggac gtgaccaagc tgggcaagcg
     1201 gctctaccgg gacgagctga agaaggtgga gcgtgacgtc agcgacggcc gcacgagcta
     1261 ccggatctac gggtacgagc ccggcctcga tgacgagccc ggtaccgaca tcggggcgat
     1321 cgcccggggc cggctgacgg tgaccccgct cagcctcgac tggacccatc acgaagaact
     1381 cgaaggcctc cgcgaccgcg acttcgagcg cctcctcgga gtggcctcgt cttgaccgac
     1441 ggggcgtcag acggctccga tcaggagcgg atttcggaac tccgggacat cctgaaccat
     1501 cacatcgtcc tctactacga gaaggacgac cccgagatat ccgacgagga gtacgacgag
     1561 cttttcacgg aacttcagcg gctcgagaag gcgaacccgg agctgatcac cggcgattcc
     1621 ccgacccagc gggtgggggc accggcttcg accagtttcg caccgg
//
LOCUS       DAOTIP010000529         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_86699_length_1664_cov_2.789932, whole genome shotgun sequence.
ACCESSION   DAOTIP010000529 DAOTIP010000000
VERSION     DAOTIP010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86699_length_1664_cov_2.789932"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="P5138_09460"
     CDS             <1..1050
                     /locus_tag="P5138_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV60845.1"
                     /translation="TAYGLSGCTVERPIDKSAAGDVVEPKIDGDLLIFNWAQYMDPAI
                     KKSFSEKYGVAVNEVNFDNLEAMVTKLRAGGSYDLIFPSTEYVQRLRNEQLIRPFDRA
                     KLKNADTLTPFYDNPWYDPNSEFSVPYAYYTTGIAWNTDEVDTTTGSWNDMSNEQAFG
                     RIFMLDDFQEGIGQANLLNGFYLNTEKSDELEVTKQTLLDQKKGLRGFSTSSVQNLVS
                     GAAALTQAWNGDVVNARNQVDDPSIFAYETCKEGVPVGSDLMCIPITSKSPGTAMVFM
                     DWVLKPENAYRNVMWNGYPMPVAGGREAFAELVKDDPSIDVNLDQLADSAMEFRLDSP
                     EARQEWTRLWTEVKA"
     gene            1047..>1664
                     /locus_tag="P5138_09465"
     CDS             1047..>1664
                     /locus_tag="P5138_09465"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV60846.1"
                     /translation="MTSKRFWDWFIIPGGVWIALLFAVPLCLVLALSFGHVDDFGRGV
                     YGFQLDNYKDVFDATYIPVLLRSVGYALATVILCLLIGYPVAYFIGRYGGRYRYALLA
                     ALVIPFFVNYLVRTYAWVALLADQGLVNNLLVDLGLTQEPIQMINTPYAVIGGLVYGY
                     IIFMILPLYASIERMDPSVVEAGKDLYGSPFQTFLHVTIPATQAGI"
BASE COUNT          321 a          546 c          502 g          295 t
ORIGIN      
        1 accgcctacg ggcttagcgg ctgcaccgtt gagcggccga tcgacaagtc ggcggcgggg
       61 gacgtggtcg aaccgaagat cgacggggac ctgctgatct tcaactgggc ccagtacatg
      121 gaccccgcga tcaagaagag cttctcggag aaatacgggg tcgcggtcaa cgaggtgaac
      181 ttcgacaacc tcgaggcgat ggtcaccaag ctgcgggccg gtggttccta tgacctgatc
      241 ttcccctcga cggaatacgt ccagcggctc cgcaacgaac agctgatccg ccccttcgac
      301 cgggcgaagc tgaagaacgc ggacacgctc accccgttct acgacaaccc ctggtacgac
      361 ccgaactccg agttttcggt gccctacgcg tactacacga ccggcatcgc ctggaacacg
      421 gacgaggtgg acacgacgac cgggtcctgg aacgacatga gcaacgaaca ggccttcggc
      481 cgtatcttca tgctcgacga tttccaggaa gggatcggcc aggccaacct gctcaacggc
      541 ttctacctca acaccgagaa gtcggacgag ctggaggtga cgaagcagac cctgctggac
      601 cagaagaagg gattgcgggg cttctccacc agctcggtcc agaacctcgt ctcgggcgcg
      661 gcggccctga cccaggcctg gaacggtgac gtggtcaacg ccagaaacca ggtcgacgac
      721 ccctcgatct tcgcctacga aacctgcaag gaaggagtgc cggtcggctc cgacctgatg
      781 tgcattccga tcacttcgaa gagccccggc acggcgatgg tgttcatgga ctgggttctc
      841 aagccggaga atgcctacag gaacgtgatg tggaacggct acccgatgcc ggtcgccggg
      901 ggacgcgagg cattcgcgga gctggtgaag gacgacccgt cgattgacgt gaacctcgat
      961 cagctggccg actcagcgat ggaattccgc ctcgacagcc ccgaggcacg ccaggaatgg
     1021 acacgcctat ggacggaggt gaaggcatga catcgaagcg cttctgggac tggttcatca
     1081 tcccgggcgg ggtgtggatc gcgctgctct tcgcggtgcc gctctgtctc gtactcgccc
     1141 tctccttcgg ccacgtcgat gacttcggcc gcggcgtcta cggcttccag ctcgacaact
     1201 acaaggacgt cttcgacgcg acctacatcc cggtcctgct gcgatcggtc gggtacgcac
     1261 tggccacggt gatcctctgc ctgctgatcg gctacccggt cgcctacttc atcggccgat
     1321 acggcggccg ttaccgctac gcgctgctgg ccgccctggt catcccgttc ttcgtcaact
     1381 acctggtccg gacctacgcc tgggtggccc tgctggcgga ccagggcctg gtcaacaacc
     1441 tgctggtcga cctcgggctg acccaggagc cgatccagat gatcaacacc ccctacgcgg
     1501 tgatcggcgg cctggtctac ggctacatca tcttcatgat cctgcctctc tacgcctcga
     1561 tcgaaaggat ggacccgagc gtggtcgagg ccggcaagga cctctacgga tcgccctttc
     1621 agaccttcct gcatgtgacc atccccgcca cccaggccgg gatc
//
LOCUS       DAOTIP010000530         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87103_length_1658_cov_3.051154, whole genome shotgun sequence.
ACCESSION   DAOTIP010000530 DAOTIP010000000
VERSION     DAOTIP010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87103_length_1658_cov_3.051154"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5138_09470"
     CDS             <1..220
                     /locus_tag="P5138_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60847.1"
                     /translation="TATIGPRAVVGVGAKVGAGARITGSVLLEGSEVGAGAVVEKSII
                     GPGVKVAAGNTVGDEVLGKNQTGEVVNA"
     gene            201..1187
                     /locus_tag="P5138_09475"
     CDS             201..1187
                     /locus_tag="P5138_09475"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRV60848.1"
                     /translation="MRSLMLEDVLDIPNHIRDALWRADSASLKARPSAGFVVCGMGGS
                     AIGAELARGILDNRLTGPMVVSRTYGLPKWVTADWAVLASSYSGDTEETIASFKDAGE
                     AGTHRWVAGTGGELGARARDAGIGVVGLPGFFQPRMTVGYMTVVAAYVAHLAGVAPDV
                     RRELEQAADFLDSCKADMEPLARALASQIGDTPVVVHGASLTTAPARRWANQFNENAK
                     QLAFAAEIPEANHNLMEAWSKGSGGLGAIFLTDSGQSARERRRMKLTAESIEKTGAQV
                     FEVETSGETRSERLFWSVMFGDLVSVEVASLRGVDPIPVDEIQNFKRRLGKT"
     gene            1247..>1658
                     /locus_tag="P5138_09480"
     CDS             1247..>1658
                     /locus_tag="P5138_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV60849.1"
                     /translation="MPKPMVPVLNRPVMEHIIDLLARHDFTEVIANIHWYPDVIKGHF
                     GDGRDFGIELTYSHEEELLGTAGGVRNASHFFDDDFLVISGDAMTDIDLAAMREFHRS
                     HGGIATLATRRVTDTSEFGVVITDEAGRIQGFQEK"
BASE COUNT          325 a          512 c          544 g          277 t
ORIGIN      
        1 gaccgcaact atcggcccca gggcggttgt aggagtgggt gccaaagtcg gcgccggagc
       61 aagaattacc ggttcggtgc tgctcgaagg aagcgaggtc ggcgccggag cggtggtcga
      121 aaagtcgatc attggacctg gagtaaaagt tgcggcaggg aatacggtag gagatgaagt
      181 ccttggcaag aatcagaccg gtgaggtcgt taatgcttga ggacgtactc gacatcccca
      241 accacatcag ggacgcgctc tggcgagccg attcggccag tctcaaggca cgcccctcgg
      301 cgggattcgt cgtctgcggc atgggtggat cggccattgg agccgagctt gcgagaggaa
      361 ttctcgacaa ccgcctgacc ggccccatgg tcgtttcccg gacctacggg ctgcccaagt
      421 gggtcaccgc cgactgggcc gtcctggcct cgagctactc cggtgacacc gaggaaacga
      481 tcgcttcctt caaggatgcc ggcgaggccg gcacccaccg ctgggtggcg ggaaccggcg
      541 gagaactcgg cgccagggca cgggacgccg gaatcggcgt ggtcggcctt cccgggttct
      601 tccagccgag aatgaccgtc ggatacatga ccgtggtcgc cgcctacgtc gcccacctgg
      661 ctggcgtggc gccggacgtg cgacgcgaac tggagcaggc ggccgacttt ctagactcct
      721 gcaaggccga catggagcct ctcgcgagag cacttgcctc gcaaataggg gacactcctg
      781 ttgtcgtgca cggagcaagc ctcacaacgg ccccggccag gcgctgggcc aaccagttca
      841 acgagaacgc gaaacaactc gcctttgcgg ccgagattcc cgaggcaaac cacaatctga
      901 tggaggcgtg gtcgaaggga agcggcggac tcggcgcgat ctttctcacc gattcgggtc
      961 agtcggcccg cgagcgtcgc cgcatgaagc tgacggcgga atcgatcgag aagacaggcg
     1021 cccaggtctt cgaagtggag acctcaggcg agacccggtc ggaacggctg ttctggtcgg
     1081 tcatgttcgg tgacctcgtg tctgtcgagg tggcatccct gcgcggagtc gaccccatcc
     1141 cggtcgacga aatccagaac ttcaagcggc gcctgggcaa gacctgatga aggcaatggt
     1201 gctggccgcc ggactgggca cgaggctcag gccagtcact cgctcagtgc ccaagccgat
     1261 ggttcccgtc ctcaaccggc cggtgatgga acacatcatc gatcttctgg ctcgccacga
     1321 cttcaccgag gtgatcgcca acatccactg gtaccccgac gtgatcaagg ggcattttgg
     1381 cgacggacgg gacttcggca tcgagctcac ctacagccat gaagaagagt tgctcggcac
     1441 cgccggcggg gtccgcaacg cttcccactt cttcgacgac gatttcctgg tcatatccgg
     1501 cgacgccatg accgacatcg acctcgccgc gatgcgcgag ttccaccgct cccacggggg
     1561 catcgcgacg ctggcgaccc ggcgggtaac ggacacctcg gaattcggtg tggtgatcac
     1621 cgatgaggcc ggacgcattc agggattcca ggagaagc
//
LOCUS       DAOTIP010000531         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87162_length_1657_cov_4.164794, whole genome shotgun sequence.
ACCESSION   DAOTIP010000531 DAOTIP010000000
VERSION     DAOTIP010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87162_length_1657_cov_4.164794"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /gene="narJ"
                     /locus_tag="P5138_09485"
     CDS             complement(<1..600)
                     /gene="narJ"
                     /locus_tag="P5138_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRV60850.1"
                     /translation="MSEQVHPLKVVSLLLQYPEPELVDALRSVDPKEVGPASGSQRAT
                     LGGFLDWYRSQDLAELRQSYVDNFDFDRRHSLHLTYQLYGDSRQRGLALLKIKNVYRD
                     ADLELSEGELPDYLPLMLEFATLAPGQEGGDLLDRHRESIELIRAGLIRSGSPWASLF
                     DVIRDGMPGLSRRQINRIKRLAEEGPPSEEVGMEPFAPPE"
     gene            complement(593..>1657)
                     /locus_tag="P5138_09490"
     CDS             complement(593..>1657)
                     /locus_tag="P5138_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60851.1"
                     /translation="KREEDGIVLVDQDACRSWRFCVSGCPYKKVYFNWNTGKAEKCNF
                     CYPRIEAGMPTVCSETCVGRLRHLGVVLIDTDRVEAAASVENEHDLLNAQLDLMLDPD
                     DPEVREQALRDGIPADWIDAASYSPVYAMAKKWKIALPLHPEYRTLPMVWYVPPLSPM
                     ESVGKALVRDGEDADAENVFAAIDEMRIPVEYLASMLSAGDVEPVRNSLQRLVAMRRY
                     MRDLNLGNEARPEIATEAGMEPHELEAMYRMVAIADYDDRYVIPKRHGEVSGDAFIDQ
                     GSCGIDFANGAPVPGMGEMAGVGAPDPFTGALAAARDGEVPETGALAPEQPDPKFDVR
                     DLLTRHGGQPGSAGAGSGNV"
BASE COUNT          263 a          560 c          551 g          283 t
ORIGIN      
        1 ttcgggcggc gcgaacggct ccatgccgac ctcctcgctc ggcgggcctt cttcggccag
       61 ccgcttgatc cggttgatct ggcgccggga gaggcccggc atcccgtccc ggatcacgtc
      121 gaagagcgac gcccaagggc ttcccgagcg gatcaggccc gcccggatca gttcgatcga
      181 ttcgcggtgc cggtcaagca agtccccgcc ttcctggccg ggcgccaggg tcgcgaactc
      241 gagcatcagc ggcaggtagt ccggcagctc gccttctgaa agctcgaggt cggcgtcgcg
      301 gtagacgttc ttgatcttga gcagggccag gccgcgctgc cggctgtcgc catagagctg
      361 gtaggtgaga tgaaggctgt gacgccggtc gaagtcgaag ttgtcgacgt aggactggcg
      421 gagctccgcg aggtcctgcg agcggtacca gtcgaggaac ccgcccagcg tcgcgcgctg
      481 ggagcccgac gcgggaccga cctctttcgg atccacgctc cgcagcgcgt cgaccagttc
      541 gggctccggg tactgcagga gcagcgagac gaccttcagc ggatgaacct gctcagacat
      601 tgccgcttcc cgctccggcc gaaccgggct ggccaccatg cctggtcagc aggtcgcgca
      661 cgtcgaactt cgggtcgggc tgttcggggg caagcgcgcc ggtttccggg acttcaccgt
      721 cccgcgcggc ggccagcgca cccgtgaacg ggtccggtgc gccgactccg gccatctctc
      781 ccatgccagg gaccggtgcc ccgttggcga aatcgatccc gcaggatccc tgatcgatga
      841 aagcgtcccc ggaaacctcg ccgtgacgct tcgggatcac gtaccggtcg tcgtaatcgg
      901 cgatcgccac catccggtac atcgcctcga gctcatgcgg ctccatgccg gcctcggttg
      961 cgatctcggg ccgggcctcg ttgccgaggt tgagatcacg catgtaccgc cgcatcgcga
     1021 ccagccgctg aagtgaattg cggaccggct cgacatcgcc ggccgagagc atcgaagcca
     1081 ggtactcgac cgggatccgc atctcgtcaa tcgccgcgaa gacattttcg gcgtcggcgt
     1141 cttcgccatc acggaccagc gccttgccga ccgattccat cggtgagagc ggcggcacgt
     1201 accagaccat cggcagcgtc cggtactcgg ggtggagcgg cagggcgatc ttccacttct
     1261 tcgccatcgc gtagacgggc gagtacgaag cggcgtcgat ccagtcggcc gggatgccgt
     1321 cccgcagtgc ctgctcgcgg acctcggggt catccggatc cagcatcagg tcgagttggg
     1381 cgttgagcag gtcatgctcg ttctcgaccg aggcagccgc ctcgacccgg tcggtgtcga
     1441 tcaggacgac gcccaggtga cgcagccggc cgacgcaggt ttccgagcag acggtcggca
     1501 tgccggcctc gatcctcggg taacagaagt tgcacttctc ggccttgccc gtgttccagt
     1561 tgaagtagac cttcttgtag gggcagccgg agacgcagaa ccgccaggag cggcaggcgt
     1621 cctggtcgac caggacgatc ccgtcctcct cgcgctt
//
LOCUS       DAOTIP010000532         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87624_length_1651_cov_3.069549, whole genome shotgun sequence.
ACCESSION   DAOTIP010000532 DAOTIP010000000
VERSION     DAOTIP010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87624_length_1651_cov_3.069549"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1651
                     /gene="nuoL"
                     /locus_tag="P5138_09495"
     CDS             13..>1651
                     /gene="nuoL"
                     /locus_tag="P5138_09495"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRV60852.1"
                     /translation="MVLLFPLLGSLTIGFGFKVLPARVAGLIGISAIVAAFICGILAL
                     IGLQGEPSESRHVASSLWEYANVGGLRIDLGIYVDPLSIFMVLVVTGVSSLIHIYSYS
                     YMKSDQGYHRFFSYLNFFVFSMLVLVLGGNFIMLIVGWALVGFASYALISYWYRRNTA
                     TKAGMKAFVINVVGDIGLYIAAILIFKNLGSVDYGTVFEKAPEVFTTNEWEIVAICLC
                     LLVGAFAKSAQMPFHTWLADAMEGPTPVSSLIHAATMVTAGVYLIARCHSLFELAPTA
                     ADVAAWVGLATLMIAGTIAIAVTDLKRIIAYSTMSQIGYMFVGVGIGAYSAGMFHLMT
                     HAFFKALLFMAAGSIISAMANMQDIDKMKGFRKAMPFTAAMLIIGALALSGFPGTSGW
                     FSKDEIIDFAHFRGGMYDWMWAGMLIGAFMTAVYSFRIIFRILPGPACKEAEYLEEHG
                     HVIHGEPKNPATGELEDTEVGYPGEEHHIAEQAGGMKFAMSVLGILALIGGLVQVPGV
                     DEVITNFLEPTFEDSVFAHMHPSTHEAWVGLGEGALIS"
BASE COUNT          269 a          552 c          487 g          343 t
ORIGIN      
        1 acctgggcat ggctggtcct gctctttccc ctgctcgggt cgctgaccat cggtttcggc
       61 ttcaaggtgc tgcccgccag ggtcgccggc ctgattggca tctcggcgat cgtggccgcg
      121 ttcatctgcg ggatcctggc cctgatcggc cttcagggag agccttcgga atcgcgccac
      181 gttgcctcca gcctctggga gtacgcaaac gtcggcggac tcaggatcga cctcgggatc
      241 tacgtcgatc cgctctcgat attcatggtg ctcgtggtca ccggcgtctc gagcctgatc
      301 cacatctact cgtacagcta catgaagtcg gaccagggct atcaccggtt cttcagttac
      361 ctgaacttct tcgtcttctc gatgctggtg ctggtcctgg gtggcaactt catcatgctg
      421 atcgtcggct gggccctggt cggctttgcc tcctacgcgt tgatcagcta ctggtaccgc
      481 cgcaacaccg ccaccaaggc cggcatgaag gccttcgtca tcaacgtggt cggtgacatc
      541 ggcctctaca tcgcggcgat cctgatcttc aagaacctcg gctcggttga ctacggcacc
      601 gtcttcgaga aggccccgga ggtcttcacc accaacgaat gggaaatcgt cgcgatctgc
      661 ctctgtctgc tggtcggagc cttcgccaag tccgcccaga tgccgttcca cacatggctc
      721 gccgacgcca tggaaggtcc gaccccggtc tcctcgctga tccatgccgc gaccatggtc
      781 accgccggcg tctacctgat cgcccgctgc cactccctct tcgagctcgc cccgaccgcc
      841 gccgacgtcg ccgcctgggt agggctcgcg accctgatga tcgccgggac gatcgcgatc
      901 gcggtgaccg acctcaaacg gatcatcgcc tactcgacca tgagccagat cggctacatg
      961 ttcgtcggcg tcggcatcgg cgcctactcg gccgggatgt tccacctgat gacgcatgct
     1021 ttcttcaagg ccctgctctt catggcggcc ggttcgatca tctcggcgat ggccaacatg
     1081 caggacatcg acaagatgaa gggctttcgc aaggcgatgc cgttcaccgc ggcgatgctg
     1141 atcatcggtg cgctggccct ctccggcttc ccgggcactt cggggtggtt ctccaaggac
     1201 gagatcatcg acttcgccca cttccgcggc ggcatgtacg actggatgtg ggccggcatg
     1261 ctgatcggcg ccttcatgac cgcggtctac tcgttccgga tcatcttccg gatcctgccc
     1321 ggtccggcct gcaaggaagc cgagtacctg gaggagcacg gtcacgtgat ccacggtgag
     1381 ccgaagaacc cggccaccgg tgagctcgag gacaccgaag tcggctaccc cggcgaggaa
     1441 catcacatcg cggagcaggc cggcggcatg aaattcgcca tgtcggtgct cggcatcctg
     1501 gccctgatcg gcggactggt acaggtcccc ggggtcgacg aggtgatcac caatttcctc
     1561 gagcccacct tcgaggattc ggtctttgcg cacatgcatc cgagcaccca tgaagcctgg
     1621 gtgggtctcg gcgagggagc cctgatctcg a
//
LOCUS       DAOTIP010000533         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87628_length_1651_cov_3.014411, whole genome shotgun sequence.
ACCESSION   DAOTIP010000533 DAOTIP010000000
VERSION     DAOTIP010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87628_length_1651_cov_3.014411"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1651)
                     /locus_tag="P5138_09500"
     CDS             complement(<1..>1651)
                     /locus_tag="P5138_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRV60853.1"
                     /translation="LRQRLIGSVVLSVPVIAMSMFEVFQFTNWQWASLTLAAPVVVWA
                     AWPFHKATWQNLRHGATTMDTLVTIGISSAFIWSLYALFFGTAGEPGMTHPFEITVAP
                     TDGTSNIYLEVASGVTMFLIAGRYFELRSKRRASSAMKALLEMGAKEAIVLRGGTEVP
                     VPIEELEVGDDFVVRPGEKLPTDGVVVSGVSSIDESMLTGEPVPVDVSEGDSVTGATV
                     NSSGRIVARATRVGSDTQLARMAKMVEDAQSGKAPVQRLADRISAFFVPAVILIAAGT
                     LGAWLGAGFPAEAAFTAAVAVLIIACPCALGLATPTALLVGTGRGARMGILIKGPEVL
                     ESTRQVDTIVLDKTGTVTAGHMELAGVVAGAGVSRRDILALAGSLENASEHPIARAIA
                     KAARSEVGELSDVEDFTNVPGRGVQGVVDGHAVLVGRETLLDDWSQRLPEQLVAAKAR
                     EEESGRTVVIVSWDGEPRGLLAVADQVKPTSGEAVRQLKGLGLDPVLLTGDNRAAARQ
                     VGDEVGIGTVLSEVLPGEKLDEIKRLQAEGRIVAMVGDGVND"
BASE COUNT          287 a          592 c          504 g          268 t
ORIGIN      
        1 atcgttcacg ccgtcaccga ccatcgcgac gatccgaccc tcggcctgga gacgcttgat
       61 ctcgtccagt ttctcgcccg gaagcacttc ggacagaacg gtcccgatcc cgacctcgtc
      121 gccgacctgc cgggcggcgg cccggttgtc cccggtaagc aacaccggat cgagcccgag
      181 gcccttcagc tgcctcaccg cttccccgct ggtcggcttg acctggtcgg cgaccgccag
      241 aagtccccgc ggttcgccgt cccaactgac gatcacgacg gttcgcccgc tttcttcctc
      301 gcgggccttg gcggcgacca gctgctccgg gaggcgctgg gaccagtcat caaggagcgt
      361 ttcgcgtccg accaggaccg cgtgaccatc gacgacgccc tgcacgccac ggccgggaac
      421 gttggtgaag tcctccacgt cggaaagctc cccgacctcg gatctcgccg ccttcgcgat
      481 cgcccgggct atcggatgtt cggaagcgtt ctccagcgag ccagccaggg ccaggatgtc
      541 ccggcgtgag actcccgctc cggccacgac accggcaagt tccatgtgtc ccgccgtgac
      601 ggtgcccgtc ttgtcgagca cgattgtgtc gacctgccgg gtcgactcga gcacctcggg
      661 tcccttgatc aggatcccca tcctggcccc gcgtccggtt ccgaccagca gggccgtcgg
      721 cgttgccagg ccgagagcgc aggggcaggc gattatgagg accgcgaccg ccgccgtgaa
      781 ggcagcctcc gcggggaagc ccgccccgag ccaggccccc agagtgcccg ccgcgatcag
      841 gatcaccgca ggtacgaaga aggccgagat ccggtccgcc agccgttgaa ccggagcctt
      901 gcccgactgg gcgtcctcga ccatcttcgc cattcgcgca agctgtgtgt cggagccgac
      961 ccgggtcgcc ctggccacta tccggccgga ggagttgacg gtagctccgg taacgctgtc
     1021 gccttcactc acatccaccg ggaccggttc gccggtcagc atcgactcgt cgatcgagga
     1081 gacccctgaa acgacaactc cgtcggtcgg caatttctcc ccggggcgaa cgacgaagtc
     1141 gtccccgacc tcgagctcct cgatcggaac gggtacctcg gttccgccac ggagcacgat
     1201 cgcctctttc gcccccatct ccagaagtgc cttcatcgcc gaactcgccc gacgcttgga
     1261 tctcagctcg aagtagcgac cggcgatcag gaacatggtc acgcctgagg cgacctcgag
     1321 gtagatgttc gaggtgccat ccgtcggagc gacggtgatc tcgaagggat gagtcatccc
     1381 gggctccccg gcagtgccga agaacagcgc gtacagcgac cagatgaagg ccgaactgat
     1441 cccgatcgtg accagcgtgt ccatcgtcgt cgctccgtgg cgaaggttct gccaggtggc
     1501 cttgtggaat ggccatgcag cccagaccac gaccggagcc gcaagcgtca gcgaggccca
     1561 ctgccagttg gtgaactgga acacctcgaa catcgacatg gcgatcaccg gaaccgagag
     1621 aacgaccgag ccgatcagcc gctgcctcag t
//
LOCUS       DAOTIP010000534         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87666_length_1651_cov_1.898496, whole genome shotgun sequence.
ACCESSION   DAOTIP010000534 DAOTIP010000000
VERSION     DAOTIP010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87666_length_1651_cov_1.898496"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5138_09505"
     CDS             <1..482
                     /locus_tag="P5138_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRV60854.1"
                     /translation="LGDDYDTDALDASVLLMPLVRFLPPDDPRIRDTVLAIREGLDDQ
                     GLILRYVTDETDDGLHGEEGTFLICSFWMVAALSEIGEKDAARKLCERLLAASGDLDL
                     YAEELEAASNRHLGNFPQAFTHLALINAVSHVIADERRGQTAPGTPTAVFTEIREMD"
     gene            479..1210
                     /locus_tag="P5138_09510"
     CDS             479..1210
                     /locus_tag="P5138_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRV60855.1"
                     /translation="MSQEPQTGATDPADAERIRTNIATVRERMATACERAGRDPDSVR
                     LLLATKTVEPERIRVAIEAGEKLVAENRVQEVRPKFEALADLEYERHFIGHLQSNKVN
                     ALVPYVSCIETLDRLSLARKLNNRLEREGETLEVLIQVNTSGEETKAGLEPDEVEPFI
                     RTVAGLKALKIRGLMTIGLNAEPEVARPSLALLRETAERIREENIDGVAMDELSMGMT
                     GDLEVAIEEGSTIVRVGSAIFGARD"
BASE COUNT          343 a          533 c          527 g          248 t
ORIGIN      
        1 ggctcggcga cgactacgac accgacgccc tcgacgcctc ggtcctcctg atgccgctgg
       61 tccgtttcct gccgccggat gacccgcgga tacgcgacac ggtcctggcg atccgggaag
      121 gactcgacga ccagggcctg atcctgcgct acgtcaccga cgagaccgac gacggtctcc
      181 atggcgaaga gggaaccttc ctgatctgct ccttctggat ggtcgccgca ctctccgaga
      241 tcggggagaa ggacgccgcc cggaagctct gcgagaggct gcttgccgcc tcaggtgatc
      301 tcgacctcta tgcggaggaa ctcgaagcgg ccagcaaccg gcacctcggc aacttccccc
      361 aggccttcac ccacctcgcc ctgatcaacg cggtctccca cgtgatcgcc gacgagcgcc
      421 ggggacagac ggcaccgggg accccgaccg cggtcttcac cgagatcagg gagatggatt
      481 gagccaggaa ccacagaccg gggcaactga tccggccgac gctgaacgga tccggaccaa
      541 catcgccacg gtcagggaga ggatggccac cgcctgcgag agagccggcc gcgacccgga
      601 ttccgtccgg ctgctgctcg caacgaagac cgttgagcca gagcggatcc gggtcgcgat
      661 cgaggccggt gagaagctgg tcgccgagaa ccgggtccag gaagtcagac ccaagttcga
      721 ggcgctggcc gacctcgaat acgaacgcca cttcatcggt catctgcagt cgaacaaggt
      781 gaacgcgctg gtcccctacg tcagttgcat cgagaccctt gaccggctct cgcttgcccg
      841 caagctgaac aaccgcctcg agcgggaggg tgaaacgctc gaggtcctga ttcaggtgaa
      901 cacctcgggc gaggagacca aggccggcct ggagcccgac gaggtcgagc cgttcatccg
      961 taccgtcgcc gggctgaaag cactgaagat ccgcggcctg atgacgatcg gactcaatgc
     1021 tgagccggag gtggcaagac cctccctggc tttacttcgg gagaccgccg agcggatccg
     1081 cgaagagaac atcgatggcg tcgcgatgga tgaactctcg atgggaatga ccggtgatct
     1141 cgaagtcgcg atcgaggagg gctcgaccat cgtgcgggtc ggcagcgcga tcttcggcgc
     1201 ccgggactga aagcgaaggg ccccggttcc ccggggccct tcggtcaaac tggctgtgcc
     1261 cgggaaactc aggcccttga gccactaccc cttcctgtga ccgccggcca gataagaagg
     1321 accaggccga tcactacgca gatgatgccc aacgcccaac tcaccagcag ggcgaggacg
     1381 attccaatga ctatgagcaa cagtgctagt ggcatgcacc gaaattaccc gcaataccgg
     1441 gcgtgaaacc cggcctcatg aatcccgcct gtggaaggct cggagccgcg gctcgaaacg
     1501 ggatcagagc cgcagcccga agaagctcta gaggtccagt tcgtagagca gatgtggaag
     1561 gccgacgtgc tcgatctctg cgacgaactg catgccagcc ttttccatga tgcggcggga
     1621 ggcgagattg gccggaaggg tcagggacac c
//
LOCUS       DAOTIP010000535         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87709_length_1650_cov_2.802508, whole genome shotgun sequence.
ACCESSION   DAOTIP010000535 DAOTIP010000000
VERSION     DAOTIP010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87709_length_1650_cov_2.802508"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="P5138_09515"
     CDS             <1..619
                     /locus_tag="P5138_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60856.1"
                     /translation="GEIEPLSGHLEVNATTATVAQTDRSRLDYPVSALDVATMGSLAR
                     LPWWKRPGRAERRRAREALAEVGLAELADSTFGDLSGGQRQRVLIARALTADAELLLM
                     DEPFTGLDTTNEQRLEELIDSLAVKGRTVMIATHEVEQAERWDLVLCLNGEQISFGDP
                     AVAISKPVLEATYGGHIVEIPGQPELGVLPAHHHHHDEGHEGAGQ"
     gene            616..1485
                     /locus_tag="P5138_09520"
     CDS             616..1485
                     /locus_tag="P5138_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRV60857.1"
                     /translation="MIDWLVDPLTHGFTVRALIEIALLGVTSGAIGCWVVLYRISYSA
                     ESLAHGMFPGLVGAALLGLPLMLGGATGIIIAGLLIAVVGRLSADAADTAIAVVVTSL
                     FGLGVLLALSPDSPPGIQALLFGDPLGITEGQLLVTALLGATVLAALWFGHDRLLAVG
                     FDGDAGRAGGPSPGLIQGLLLILVAVTVLVGVQGLGNLLVAAILVGPAAAARLLTDRM
                     KPMILLSAGIAVLSGVAGLYLSFHGRVAAGAAIVACILLAYLSAAFLAAAKTRLVRPT
                     RKETPDEPVTQLV"
     gene            1460..>1650
                     /locus_tag="P5138_09525"
     CDS             1460..>1650
                     /locus_tag="P5138_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60858.1"
                     /translation="MSQSPSWSEYSARRIAEAGLRRSIPRRRVIDLLAERDCAVTALE
                     IDAELDGVGRATVYRAIEQL"
BASE COUNT          234 a          595 c          540 g          281 t
ORIGIN      
        1 gggcgagatc gagccgcttt ccggccatct ggaggtcaat gccaccaccg ccacggtggc
       61 ccagaccgac cggtcccggc tcgattaccc cgtctccgcg cttgacgtgg ccaccatggg
      121 ttcgctggcg aggctgccct ggtggaagcg tccgggccgg gcagaaaggc gtcgggcccg
      181 ggaggcactg gccgaggtgg gtctggccga actcgccgac agcaccttcg gagatctttc
      241 cggcggccag cgccagcgcg tcctgatcgc ccgcgcgctc accgccgacg ccgaactgct
      301 gctgatggac gagcccttca cgggcctgga tacgaccaat gaacagcggc tcgaagagct
      361 gatcgacagt ctcgctgtca aaggccgcac ggtcatgatc gccacgcatg aggtcgagca
      421 ggccgaacgc tgggacctgg tgctctgcct gaacggcgaa cagatctcct tcggagaccc
      481 ggcggtcgcg atcagcaagc cggtgctgga agccacctac ggcggccaca tcgttgagat
      541 ccccggccag cccgagctcg gtgtgctccc cgcgcaccat caccatcacg atgagggcca
      601 cgaaggagca gggcaatgat cgactggctg gtcgaccccc tcacccacgg gttcacggtt
      661 cgcgccctga tcgagatcgc cctgctcggc gtgaccagcg gcgcgatcgg ctgctgggtg
      721 gtcctctacc gcatctccta cagcgccgaa tcgctcgctc acggcatgtt tcccggcctg
      781 gtcggagccg ccctgctggg gctgccgctc atgctcggcg gcgccaccgg catcatcatc
      841 gccggactgc tgatcgcggt cgtcggccgg ctctccgccg acgcggccga cacggcgatc
      901 gccgtcgtgg tcacctcgct cttcgggctc ggggtgctgc tggcgctttc gcccgacagt
      961 cccccgggca ttcaggccct cctcttcggc gatccgctcg gaatcaccga ggggcaactc
     1021 ctcgtgaccg ccctgctcgg cgccacggtc ctggccgctc tctggttcgg gcacgaccgg
     1081 ttgctggccg tcgggttcga cggtgacgcc ggccgcgcgg gcggtccttc gccgggcctg
     1141 atccagggcc tgctgctcat cctcgtcgcg gtgactgtgc tggtcggggt tcagggcctc
     1201 ggaaacctgc tcgtggcggc aatcctggtc ggtccggccg cagccgcccg gctgctgacc
     1261 gaccggatga aaccgatgat cctgctcagc gccggtattg ccgttctctc cggagttgcc
     1321 ggcctctacc tgtcttttca cggccgggtc gccgccggcg ccgcgatcgt cgcctgcatc
     1381 cttctcgcct acctgagcgc cgcgttcctg gcggcggcga aaaccagact tgtacgcccg
     1441 acccgaaagg agactcccga tgagccagtc acccagctgg tctgaatatt cggcccgacg
     1501 gatcgccgag gccggcctgc gtcgcagcat cccccggcga cgggtgatcg acctgctggc
     1561 ggaacgggat tgcgccgtaa ccgctctcga gattgatgcc gaactggacg gggtcggccg
     1621 cgccaccgtc taccgggcga tcgaacagct
//
LOCUS       DAOTIP010000536         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_87855_length_1648_cov_2.671689, whole genome shotgun sequence.
ACCESSION   DAOTIP010000536 DAOTIP010000000
VERSION     DAOTIP010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87855_length_1648_cov_2.671689"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1155
                     /locus_tag="P5138_09530"
     CDS             148..1155
                     /locus_tag="P5138_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRV60859.1"
                     /translation="MSEDRTNGGTGDAYSVADQITSYQQSALIGTAVEVGLPAVLYGS
                     PKTLDELTGALGTDPRGTMGLLSGLVTFGLVERDGDRFSLTADGELLADDHPDSIAMI
                     ARKEWFFYKLWANMPEAVRTGHTQTRAWRDRLENDPEQAHDFLRALDDLCRLYGGDLP
                     GLADLESGGRLLDVGGGAGSHSANLLAAVDGLEATVLDLPGAEPVLRERHPELSFVEG
                     DIDQPRFGRPEGEEWDYVLVSNILHDHPAGENERIVREAAGLLAPGGSLVIYEWVIDE
                     GRTSPAGVASFTPMMVIENEGGYTWTEAEIGGWIRSAGLEPRPISQGPGPIAVIRAER
                     A"
     gene            complement(1152..>1648)
                     /locus_tag="P5138_09535"
     CDS             complement(1152..>1648)
                     /locus_tag="P5138_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60860.1"
                     /translation="GDSIGSFKVPRGQYRITLIDPKKITCVQAFKRFKEFLLDFDGRL
                     PYPWKLNPLMGIFYNSKHGRIGFKVNKAYGPAPRPNKHTYVRCPATFRVLNNDRIGAL
                     YLRKGPYYVYVVNGLSCSSASNYFRQFLNRTDGRLPRPWRLNVAKAKFNTGKGGVAFR
                     VQHA"
BASE COUNT          290 a          523 c          560 g          275 t
ORIGIN      
        1 tatcaccgga atgccgggaa ggaacgcggc cggagtgatc ctcgcggaat tgggtctcgc
       61 gaagcggacc cggggcggaa aactcaaagc gcaggcggcg ctcctgaagg atgccctccg
      121 ggccacgaga gaattgagga agaacgcatg agtgaagacc ggaccaacgg tggcacaggc
      181 gacgcctact cggtcgccga ccagatcacc tcctaccagc agtcggccct gatcggaact
      241 gcggttgaag tcggtcttcc ggccgtgctc tacgggtctc cgaagaccct cgacgagctg
      301 accggggccc ttggcaccga tccgcgcgga accatgggcc tgctttccgg tctggtcacc
      361 ttcggcctgg tcgaacggga cggcgaccgg ttttccctga ccgccgacgg ggaactgctg
      421 gccgacgacc accccgattc gatcgcaatg atcgcccgga aggaatggtt cttctacaag
      481 ctctgggcga acatgcccga ggcggtccgg accggtcaca cccagacccg ggcctggcgc
      541 gatcgcctgg agaatgaccc cgagcaggcc catgacttcc tgagggcgct ggatgacctc
      601 tgccggctct acggcggcga ccttcccggg ctggcggacc tcgagtcggg cggtcgcctg
      661 ctcgatgtcg gcggcggggc cggttcccat tcggccaacc tgctcgccgc ggtcgacggt
      721 ctcgaggcca cggtgctcga cctgcctggg gcggagccgg tcctgaggga acgccacccg
      781 gaactctcct tcgtggaggg agacatcgac cagccccgct tcggccggcc cgaaggggag
      841 gagtgggatt acgtcctcgt atccaacatc ctccacgacc atccggccgg ggaaaacgaa
      901 cggatcgtca gggaagcagc tggtctgctc gcccccggag gctcgctggt catctacgaa
      961 tgggtgatcg acgagggccg cacctcaccg gccggcgtcg cttcgttcac cccgatgatg
     1021 gtgatcgaaa acgaaggcgg ctacacctgg accgaagccg aaatcggcgg gtggatcagg
     1081 tcagcaggtc tggagccccg gccgataagc cagggccccg gacccatcgc agtaatcagg
     1141 gccgagcgcg cctaggcgtg ctgtacgcgg aaggccactc cgcccttgcc ggtgttgaac
     1201 ttggccttgg ccacgttcag gcgccacgga cgcggcagcc tgccgtcggt ccggttcagg
     1261 aactggcgga agtagtttga agcgctgctg cagctgagac cgttgaccac gtagacgtag
     1321 tagggcccct ttcgcaggta gagcgcaccg atgcggtcgt tgttcaggac ccggaaggtc
     1381 gcggggcagc ggacgtaggt gtgcttgttc ggcctcggcg cgggcccgta ggccttgttc
     1441 accttgaagc cgatccggcc gtgcttgctg ttgtagaaga tgcccatcag cggattcagc
     1501 ttccacgggt aggggaggcg gccgtcgaaa tcgagcagga actccttgaa gcgcttgaat
     1561 gcctggacgc aggtgatctt cttcgggtcg atcagggtga tgcggtactg gccccgcggc
     1621 accttgaagc tgccgatcga gtcgcccc
//
LOCUS       DAOTIP010000537         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_88135_length_1644_cov_3.666457, whole genome shotgun sequence.
ACCESSION   DAOTIP010000537 DAOTIP010000000
VERSION     DAOTIP010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88135_length_1644_cov_3.666457"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="P5138_09540"
     CDS             <1..218
                     /locus_tag="P5138_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60861.1"
                     /translation="PQSHHAVALAALRAGKSASTQIKVRVTRKARPGTRVKLQVAVTA
                     NGTKKKTASVSLLGKPGGMTSSSYAAG"
     gene            complement(304..867)
                     /locus_tag="P5138_09545"
     CDS             complement(304..867)
                     /locus_tag="P5138_09545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRV60862.1"
                     /translation="MINLLVVIASVRPGRVGEPVANWFVPIAEADERFNVEVADLKVL
                     DLPFMDEPNMPGRREYTKEHTKKWSAIVDRADAFVFVTPEYNATPAPALKNAIDYLYH
                     EWAFKPASFVSYGGVWAGARAVQALKPTLTQLSVMPVPEMVPIPGVAGLIDEEGNFQA
                     DERHEKGAATMLGSLAKWSGALETLRQ"
     gene            complement(920..993)
                     /locus_tag="P5138_09550"
     tRNA            complement(920..993)
                     /locus_tag="P5138_09550"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(957..959),aa:Asp,seq:gtc)
     gene            1121..>1644
                     /locus_tag="P5138_09555"
     CDS             1121..>1644
                     /locus_tag="P5138_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRV60863.1"
                     /translation="MRLVTYASDRGPRAGVQTNAGVIDAWDLIGGEAGGSLRELIASG
                     RVDEVKSAIEAGEGEAITPGPGAILAPIPDPDKIICVGLNYRAHAEETGMELPKAPTI
                     FGKYRNALVPDGASVTLPAASSKVDYEAEVAFVIGRRASEVSVEDALDHVAGYTLLND
                     LSARDLQGLTMQWMA"
BASE COUNT          294 a          550 c          551 g          249 t
ORIGIN      
        1 cgccgcagtc gcaccacgcg gtcgctctgg ccgccctgcg ggccggcaag tcggcgtcga
       61 cgcagatcaa ggtccgggtc actcgcaagg ccaggcctgg tacgagggtg aagctccagg
      121 ttgcggtcac cgccaacggg acgaagaaga agacggcgtc agtctccctc cttgggaagc
      181 cgggcggaat gacctcgagc agctacgccg cgggatgacc ccgggcatcc acgcctgatt
      241 cgggaggtga cgcaggtcta gaccccatgt gaggtccaaa cctgcgtcac ctcccctttc
      301 cggttactgc cggagcgtct ccagggcgcc tgaccacttc gccagcgagc cgagcatggt
      361 cgcggcgccc ttctcatgcc tctcgtcggc ctggaagttg ccctcctcgt cgatcagccc
      421 ggcgacgccc gggatcggca ccatctccgg gaccggcatg accgaaagct gggtcagggt
      481 cggcttcagc gcctgcaccg cgcgggcacc cgcccagacc ccgccgtaac tcacgaacga
      541 agcgggcttg aaggcccact cgtggtagag gtagtcgatc gcgttcttca gggccggggc
      601 aggggtcgcg ttgtattcgg gggtgacgaa gacaaaggca tcggcccggt cgacgatcgc
      661 actccacttc ttcgtgtgtt ccttcgtgta ctcgcgacgt ccgggcatgt tcggctcatc
      721 catgaaaggc aggtccagga ccttcaggtc ggcgacttcc acgttgaagc gttcgtccgc
      781 ttcggcgatc ggaacgaacc agttggcgac cggctcaccg acccggccgg ggcgaacgct
      841 ggcgattacg acgaggagat tgatcatgcg gcgagcctac tgcccaccgg acggagcgga
      901 cagcgtgctg ccggatgaac gggagcgacg ggacttgaac ccgcggcctc tggcgtgaca
      961 ggccagcgct ctaaccaact gagctacgcc cccgtgaggc gatcgatcga aatcgaccgg
     1021 gcgggcatta tcgcagtctc gatcgaatca cggcatctga ccccgggcgg ccgggggcgg
     1081 atcgggcgga ggctgccacg caaccactag gctggcggcc atgagactcg tcacctacgc
     1141 atcagaccgg ggcccgcggg ccggagtcca gaccaacgca ggcgtgatcg atgcctggga
     1201 cctgatcggc ggggaagccg gcgggagcct cagggaactg atcgcctcgg gccgcgtcga
     1261 cgaggtgaag tccgcgatcg aagccggtga aggagaggcg atcacgccgg gcccgggagc
     1321 cattctcgcc ccgattcccg acccggacaa gatcatctgc gtcggtctca actaccgggc
     1381 acatgccgag gagaccggca tggaactgcc caaggccccg acgattttcg gcaagtaccg
     1441 gaacgccctg gtgccggacg gggcctccgt gaccctgccc gcagccagca gcaaggtcga
     1501 ctacgaggcc gaggtggcct tcgtcatcgg ccggcgtgcc agcgaggtca gcgtcgagga
     1561 cgccctcgat cacgtcgccg gctacacgct gctgaacgac ctttccgccc gcgacctcca
     1621 ggggctgacc atgcaatgga tggc
//
LOCUS       DAOTIP010000538         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_88396_length_1641_cov_3.192308, whole genome shotgun sequence.
ACCESSION   DAOTIP010000538 DAOTIP010000000
VERSION     DAOTIP010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88396_length_1641_cov_3.192308"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="P5138_09560"
     CDS             complement(<1..572)
                     /locus_tag="P5138_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60864.1"
                     /translation="MDLSTDICGIELSGPVLNGSGTFDALAARRVFGDSLIEDFPFDC
                     YVSKTITMNPRPGNPPYRLWETPSGMINSIGLPGPGLDGFLERDLPGLAGLGVPLIVS
                     VMADQVEDFEVLVSRVATRDEVAGIELNVSCPNVHSGLIVGEQPSETEALMKALRPIT
                     EKPLIVKLTPNVANPQEVAVGAEAGGADAVS"
     gene            complement(583..1461)
                     /locus_tag="P5138_09565"
     CDS             complement(583..1461)
                     /locus_tag="P5138_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60865.1"
                     /translation="MSADSGGKPGRTQAPFGRRLCEVADSRPAGGYRIFTLIDEDGPS
                     PGPGQFYMLAAHPDWAAEGGRPYLPRAISHARVSGNPAGGQRLDFLIDEVGPGTSRLA
                     AMSPGDACWITGPFGGRFSLPRQLNPKAAGAVLVGGGIGIAPMAVLRRELVDRGIPSR
                     SLLGFRDREHSGGIDELFGCEETRLAFEDGSSGHKGYVTDLLLQILEGDDSGSAAVYS
                     CGPPAMLEAVRRICLDRGIPVELALESPMACGYGACFGCAVPKADGGYMRLCIDGPVV
                     RGDEIATAMAGEPAVG"
     gene            complement(1451..>1641)
                     /locus_tag="P5138_09570"
     CDS             complement(1451..>1641)
                     /locus_tag="P5138_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60866.1"
                     /translation="LVINTPTGSGARADGYEIRSAAIRHGIPSVTTLTGASAAVRAMA
                     ARANGYGEVMSLQELHVR"
BASE COUNT          284 a          564 c          544 g          249 t
ORIGIN      
        1 gagacggcat cggccccgcc ggcctcggcc ccgaccgcca cctcctgggg gttggcgaca
       61 ttcggagtca gcttcacgat caaaggcttt tccgtgatcg gacgaagcgc cttcatcagg
      121 gcctctgtct ccgatggctg ctcgcccacg atcaggcccg agtggacgtt cgggcaggaa
      181 acgttcagtt cgatcccggc aacctcgtcg cgggtcgcaa cccgactcac caggacctcg
      241 aaatcttcaa cctgatcggc catcaccgag acgatcaacg gcactccgag cccggccagt
      301 cctggcaggt cccgctccag gaagccgtca agcccgggac cgggcaggcc gatcgagttg
      361 atcatccctg aaggcgtctc ccacagccgg tagggcggat tgcccgggcg cgggttcatg
      421 gtgatcgtct tggagacgta acaatcgaag gggaaatcct cgatcaggga gtcaccgaag
      481 actctccggg cggccagcgc gtcgaaggtg ccggaaccgt tcagaaccgg ccctgacagc
      541 tcgatgccgc agatgtccgt ggacaggtcc atggtggtcc gttcagccaa cggccggctc
      601 ccccgccatc gcggtggcga tctcgtcgcc acggacgacc gggccgtcga tgcagagcct
      661 catgtacccc ccgtccgcct tcggcacggc gcagccgaag caggcaccgt acccgcaggc
      721 catcggtgac tcgagggcca actcgaccgg aatcccgcga tcgagacaga tccgacgcac
      781 cgcttcgagc atggcgggcg gcccgcagga atagacggcg gccgacccgg agtcgtctcc
      841 ttccaggatc tgcagcagga gatcggtgac atatcccttg tgaccggaac tgccgtcctc
      901 gaaggcgagc cgggtttcct cgcaaccgaa gagctcgtcg attccgccgg agtgctcccg
      961 gtctcggaag ccgagcagcg aacgggacgg aatgccccgg tcgaccagct cgcggcgcaa
     1021 cactgccatc ggggcgatgc ctattccgcc acccacgaga acggcgcccg ccgccttcgg
     1081 gttgagctgc ctgggcagac tgaagcgacc gccgaagggg ccggtgatcc agcaggcgtc
     1141 gcccgggctc atggcggcga gcctcgaggt gccgggaccg acttcgtcga tcaggaagtc
     1201 gagtcgctgc ccgccggccg gattcccgct gaccctggcg tgggagatcg cccggggcaa
     1261 atacggcctt cctccctctg ccgcccagtc gggatgggcg gccagcatgt agaactgacc
     1321 cggcccgggg gaaggtccgt cttcgtcgat cagggtgaag atcctgtagc cgccggcagg
     1381 cctcgaatcg gcgacttcac agaggcgccg gccgaacggc gcctgggtcc ggccgggctt
     1441 gccgccggaa tcagcggaca tggagctcct ggagcgacat cacttccccg tagccgttgg
     1501 cccgggcggc catcgcccgc acggccgccg atgcgccggt cagggtggtg accgacggga
     1561 ttccgtgccg aattgccgcc gagcggattt cataaccgtc ggcgcgggcc ccggaaccgg
     1621 tcggggtgtt gatcacgaga t
//
LOCUS       DAOTIP010000539         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_88403_length_1641_cov_3.010088, whole genome shotgun sequence.
ACCESSION   DAOTIP010000539 DAOTIP010000000
VERSION     DAOTIP010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88403_length_1641_cov_3.010088"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="P5138_09575"
     CDS             <1..320
                     /locus_tag="P5138_09575"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRV60867.1"
                     /translation="GTPTIGIGAGNVTAGQVLVLHDLTGINHGHVAKFVKRYSDVRSE
                     MLRGVEAYVEEVRSGAFPAPEHVYSVDPSELEPLRQRLNREDLENASYLESNQSWDWE
                     PLS"
     gene            complement(290..901)
                     /locus_tag="P5138_09580"
     CDS             complement(290..901)
                     /locus_tag="P5138_09580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRV60868.1"
                     /translation="MSDQRGLRMWWYKRRWYERSHRPLRRLRLDLEMARRGSYVRHPI
                     EGDILGPLRSGRLVIGQGCHFEPGCWVTMSDHARVTLGDDVWLNRNVMLAAENEVSIG
                     DHVMFANGCFIGDASHRFNDPTIPITWQGFTSKGPVRIGSNVWLGVNCVVTSGVTIGD
                     HCVVGANSVVTKDLPDGTIAAGAPAEVIREVEFLQDSGSQSQD"
     gene            962..>1641
                     /locus_tag="P5138_09585"
     CDS             962..>1641
                     /locus_tag="P5138_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60869.1"
                     /translation="MLRRKVDQTDEAAAAAKAPEGDAAGQETAAGENAEAVKADTAEP
                     RTEPAPEAEAAPEEAAEASSEPEPEASPEPEPETGPESERAGSDQPTEVEPAGILEED
                     VRDEEIEVTETPDAPTPPPSPGPAAVHSPEPQTAAERMAVRQQGIEADPEKERKALLK
                     ERREASKLQRKADKEKRRADRRAAKEARKARHKSGGDTEPLDLDPLSPTSPENPERPP
                     NPPAGSPAA"
BASE COUNT          327 a          541 c          548 g          225 t
ORIGIN      
        1 ttggcactcc gacgatcggg atcggggccg gcaacgtaac cgccgggcag gttctggtgc
       61 ttcacgacct gaccgggatc aaccacgggc atgtggccaa gttcgtcaag cgctactcgg
      121 atgttcgctc ggagatgctg cgaggagtcg aggcctatgt ggaagaggtc cgcagcggcg
      181 ccttccccgc cccggaacac gtctacagcg tcgatccgtc ggagctggag ccgctgaggc
      241 agcgcctcaa ccgggaagac ctcgaaaacg cctcgtacct ggagtcgaat cagtcctggg
      301 actgggagcc gctgtcctga agaaactcga cttcccggat cacctcggcg ggggctccgg
      361 cggcgatcgt gccgtcgggc aggtccttgg tgacgaccga gtttgctcct actacgcagt
      421 ggtcaccgat tgtcacgcct gatgtgacca cgcagttgac cccgagccag acgttggagc
      481 cgatccgtac cgggcccttg ctggtgaagc cctgccaggt gatcggaatc gttggatcgt
      541 tgaagcggtg gcttgcgtca ccgatgaagc agccgttcgc gaacatgacg tgatccccga
      601 tcgagacctc gttctcggcg gccagcatca cgttccggtt cagccagacg tcgtcgccga
      661 gcgtgacccg ggcgtgatct gacatggtca cccagcaacc gggctcgaaa tgacatccct
      721 gcccgatcac cagacgaccg gaccgcagcg gccccaggat gtcgccctcg atcgggtgcc
      781 tgacgtagga gccgcgacgg gccatttcca ggtccaggcg gagacgcctc agagggcgat
      841 gtgaacgctc gtaccagcgt cgcttgtacc accacatccg cagtccgcgc tggtcgctca
      901 tgccaatacc ttgccagagg tggatagggt gcggccatga cggcgatgaa ggtgttctct
      961 gatgctcagg cgtaaggtcg accagacgga cgaagcggcg gccgcggcga aggcgccgga
     1021 aggcgatgcc gccggtcagg aaactgcggc cggggagaat gcggaagccg tgaaggccga
     1081 tacggccgaa ccgcggaccg aaccggcacc cgaagcggag gcggcgcccg aagaagcggc
     1141 tgaagcctcc tccgagccgg agcccgaagc ttcccccgaa cccgagccgg aaaccgggcc
     1201 cgaatcggaa cgggccgggt ccgaccagcc gaccgaggtc gaacccgccg gcatcctcga
     1261 agaagacgtt cgtgacgagg aaatcgaagt gaccgaaacg ccggatgccc cgaccccgcc
     1321 gccttcgccg ggaccagccg cggtccattc acccgagccg cagacagcgg cggaacgcat
     1381 ggcagtgcgt cagcagggca tcgaggccga tcccgaaaag gaacgcaagg ccctgttgaa
     1441 ggaacgcagg gaggcctcga agcttcagcg gaaggctgac aaggagaagc gcagggccga
     1501 caggagagcc gccaaggagg cccgcaaggc gcggcacaag tccggaggcg atacggagcc
     1561 cctggatctc gacccgctct cccccacctc gcccgagaat ccggagcgcc cgccaaaccc
     1621 gcccgccggg tcgcccgcag c
//
LOCUS       DAOTIP010000540         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_88858_length_1635_cov_3.515823, whole genome shotgun sequence.
ACCESSION   DAOTIP010000540 DAOTIP010000000
VERSION     DAOTIP010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88858_length_1635_cov_3.515823"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="P5138_09590"
     CDS             <1..195
                     /locus_tag="P5138_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV60870.1"
                     /translation="DTPVSQLSIAQQQMVEIAGAVSHNAKVVIMDEPTSSLTAKEIDE
                     LFEAGVAAGPGAGGATEFRA"
     gene            192..905
                     /locus_tag="P5138_09595"
     CDS             192..905
                     /locus_tag="P5138_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRV60871.1"
                     /translation="MSQAATGEGRASANGELLKMSGLVEASGVPAPTIKHYLREGLLP
                     EPVKTSRNMAWYRPETVERIRLIKRLQEERFMPLKAIRALFEEDPGQAAAMLDVEDRI
                     LERALARERTRTSASEVKKYGVPKEVLDRLAEIGILTPNSRGYSPSDLTIIEAISRFR
                     AGGYDERIGFTVYDTLRYKRALETLVKEEVDVLMDRLDGEMPPERAVELIEAGVEPLK
                     DLIAALHTKLMVEEVERRR"
     gene            1059..>1635
                     /locus_tag="P5138_09600"
     CDS             1059..>1635
                     /locus_tag="P5138_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60872.1"
                     /translation="MAKGSPGTPSTDYPRFNAYPANQGPNRVVYSFEVSQDFASSRWP
                     RPRAVVLQLKAASRWKSVRVKKFRLKGGYSGTMRIGAALSRSHPRIQARLLLRGGKRV
                     LLGSEPVLVKVSSPKLSARSVSIGSGHACAILPSRMAACWGDNFYGQLGTGDTRDRLA
                     PVRVKGLGRVRAIEASEGFTCAIRITARVMCW"
BASE COUNT          306 a          508 c          558 g          263 t
ORIGIN      
        1 gacaccccgg tcagtcaact ctccatcgcg cagcagcaga tggtcgaaat cgccggcgct
       61 gtttcgcata acgccaaggt cgtcatcatg gacgaaccga cttcgtccct gaccgcgaag
      121 gagatcgacg agctcttcga agcgggcgtc gcggccggac cgggcgccgg gggagcgacg
      181 gagttccggg catgagccag gcagccaccg gggaaggccg cgcatcggcc aacggggagc
      241 tgctgaagat gtccgggctg gtcgaggcgt cgggtgtccc ggccccgacc atcaagcact
      301 acctgcgcga ggggctcctt cccgaaccgg tcaagacgag ccggaacatg gcctggtacc
      361 ggccggagac ggtcgagcgg atccggttga tcaagcggct gcaggaggaa cgcttcatgc
      421 cgttgaaggc gatccgggcc ctcttcgagg aggaccccgg ccaggccgcc gcgatgctgg
      481 atgtcgagga caggattctg gagcgggccc tggcccggga acggacccgg accagcgcct
      541 ccgaggtcaa gaagtacggg gtaccgaagg aagtcctgga ccgtctggcc gagatcggga
      601 tcctcacccc gaattcgcgt ggctattcgc catccgatct gaccatcatc gaggcgatct
      661 cacgcttccg ggccgggggc tacgacgagc ggatcggctt cacggtctac gacacgctgc
      721 gctacaagcg ggcgctggag accctcgtca aggaggaagt cgacgtgctg atggaccgtc
      781 tcgatggcga gatgcccccg gaaagggcag tcgagctgat cgaggccggc gtcgaaccgc
      841 tcaaggacct gatcgccgcg ctccacacca agctgatggt cgaagaggta gaacgccgcc
      901 gctgaggtct cctcgggatg tgcgagaatt tccccgtcca ggacggatgg gggagacagt
      961 tgaatcagtt cagcgggaga acaatcaggg acggtggtcg gtcggcaggc ctcattgccc
     1021 tgctcctcct gtgcctgggc tgcggactct tcctgccgat ggcgaagggg agcccgggga
     1081 ctccgagcac tgactatccg aggttcaacg cctatccggc gaatcaggga cccaaccggg
     1141 tcgtctactc cttcgaggta agccaggatt tcgcgtcgtc caggtggcca cgaccacggg
     1201 ccgtggtgct ccagctaaag gccgccagtc gctggaagtc ggtacgggtg aagaagttcc
     1261 gcctgaaagg cggatactcc ggaaccatgc ggataggtgc ggccctgtcc cgctcccacc
     1321 cccgcattca ggcgagactg ttgctgcgcg gaggaaagcg cgtcctgctg ggcagcgaac
     1381 cggtattggt caaggtttcc agcccgaagc tttcggcccg gtccgtctcg atcggcagcg
     1441 gacacgcctg cgccatcctg ccttcccgaa tggccgcctg ctggggtgac aacttctacg
     1501 ggcaactcgg caccggcgat acccgcgaca gactcgcccc cgtgcgagtg aagggtctgg
     1561 gacgtgtaag ggcgatcgaa gcaagcgagg gtttcacttg tgcgatcagg atcaccgccc
     1621 gggtcatgtg ctggg
//
LOCUS       DAOTIP010000541         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_88859_length_1635_cov_3.506962, whole genome shotgun sequence.
ACCESSION   DAOTIP010000541 DAOTIP010000000
VERSION     DAOTIP010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88859_length_1635_cov_3.506962"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..889
                     /locus_tag="P5138_09605"
     CDS             257..889
                     /locus_tag="P5138_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HRV60873.1"
                     /translation="MPIAFKEWAVTVRALAEGEQLLTLRKGGIREENKHFELEHDRFF
                     LYPTFDHQQNDLVRASHHPELDRALEEGVWPDEEPPARALLQDGGIPQPDRVRIRAWA
                     EVAEDITIEDPRIVDALSPFYIWTNDYAHKRLNWKPRHPLHLVLLRVHRIPRPVTVRV
                     REEYHGCRSWVEIDRDLPFEGTPVMSDEEFEFATSEIRSICAGDREPALV"
     gene            960..>1635
                     /locus_tag="P5138_09610"
     CDS             960..>1635
                     /locus_tag="P5138_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60874.1"
                     /translation="MTWIETTSLTFTARHEDADRTYAEELLDRLEDLSLKLEDRFEVV
                     PGDVSIVIHPSPVWLTAAHPFLPFVRYAAAPAGRRYLAGWPMATEVHVLGEKAMEKRA
                     AGEASAEALRGTAERLYTQIVLAANNERIPPPWTPMRFYRYLSWAWLVEGGAQHFARQ
                     VDFFRPAVIRRLREGSRPSFPPSRRDAVVLGGTIFDLLEDYRGGGACELLIGRLRRDG
                     PIANLEV"
BASE COUNT          272 a          580 c          494 g          289 t
ORIGIN      
        1 aagccaccgc atacgtcccg gccggctgga gactgaccgg ttcggccggc ggcctgctcc
       61 acatgtttcg tgaacgctga gttcgtgaat gcgggctact acgtaatttt cggcacgagg
      121 acttgaattt cgccgatccg gtctgtaaag tggcaaaccc ttacggggcc cgcggcagcc
      181 agtcatgcct cctctcgccc cgtacaggct cccgaagtac ctgtccgtag caacctcaac
      241 ccctgactga atacctatgc caattgcgtt caaggaatgg gctgtcaccg tgcgagccct
      301 cgccgaaggc gaacagctcc tcaccctccg aaagggaggc atccgtgagg agaacaagca
      361 cttcgaactc gagcacgacc gcttcttcct ctaccccacc ttcgaccatc agcaaaacga
      421 cctggtacgt gcctcccatc atccggagct ggaccgtgcc ctcgaggagg gcgtctggcc
      481 cgacgaggag cccccggccc gtgccctcct gcaggacggc gggatccccc agcccgaccg
      541 ggtccgcatc cgcgcctggg cggaagtagc tgaagacatc acgatcgagg accctcgcat
      601 cgtcgatgct ctgtccccct tctacatctg gaccaatgac tacgcgcaca agcgcctgaa
      661 ctggaagcct cgccatccgc tgcatctggt tctgctgcgg gtccaccgca tcccgcgccc
      721 ggtgaccgta cgggtccgcg aggaatacca cggctgccgc tcctgggtcg agatcgatcg
      781 tgatctcccc ttcgagggca ccccggtcat gtccgacgag gaattcgagt tcgcgaccag
      841 cgagatccgt tcgatctgcg ccggcgaccg cgagcccgcc ctggtctagg caccgccggt
      901 tgtgtttcat gagaagggcc cgcttcggcg ggcctttttc attgcaatag gctgcgccgg
      961 tgacgtggat cgaaaccact tctctcacct tcacggcgcg gcatgaggat gccgaccgga
     1021 cctatgccga ggaactgctc gaccggctcg aggacctcag cctgaagctt gaagaccgct
     1081 tcgaggtggt ccccggcgac gtgtcgatcg tgatccatcc ctcgccggtc tggctgaccg
     1141 ccgctcaccc cttccttccc ttcgtccggt acgccgccgc accggccggc cgccggtacc
     1201 tggccggatg gccgatggcg acggaggttc acgtgctggg cgagaaggcg atggagaagc
     1261 gggctgctgg cgaagcctcc gccgaggcac tgcgcggaac ggccgagcgc ctctacaccc
     1321 agatcgtgct ggcggccaac aacgagcgga tcccgccccc gtggacgccg atgcgtttct
     1381 accgttacct gagctgggcc tggctggtcg agggcggcgc ccagcacttt gcccgccagg
     1441 tcgatttctt ccggccagcg gtgatccgcc gacttcgtga gggttcgcgc ccgagcttcc
     1501 caccctcccg ccgcgacgcg gtggtactcg gcggcacgat cttcgacctg ctcgaggact
     1561 accgcggcgg cggtgcctgc gaactgctga tcggccgcct gcgccgcgac ggaccgatcg
     1621 ccaacctcga ggtcg
//
LOCUS       DAOTIP010000542         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_88982_length_1634_cov_2.540849, whole genome shotgun sequence.
ACCESSION   DAOTIP010000542 DAOTIP010000000
VERSION     DAOTIP010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88982_length_1634_cov_2.540849"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..>1634
                     /locus_tag="P5138_09615"
     CDS             205..>1634
                     /locus_tag="P5138_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60875.1"
                     /translation="MTTGNQKTRGILIALALLLGALMMFGPARAEAAGYPNISMSKTT
                     SGSVLLGGNSNVTLTASNPNGQPTGYNLSFRDVLPPGISYVPGSSPSSTGEPQVIANA
                     PAAGQTTLIWSNVADLTPNSTFTFTFQVTNSPAVFAIGDTYQNTAGSYLNCDPRYVPK
                     FNAQGQPTQSGGNATCSGLPQETSYTGSATASANTLIKAIEIDKSEPSPEGQLLRGLH
                     DHQTVYTLTVTNNEVDADDNVKVEDYLPAGLEFLGCGTQDNTTDAPTNPGSAEEYPGS
                     GAINPGNAPAAPDCVVPDTVETVLNPSGLPAGVYTHVVWNNLGNLGPSGQLRIQYVAA
                     VPIRQNTMTWTNGVPPVTGTQGSNLDNNSGPETTHLQGLTNHAKVTSDHQGPGGPTEV
                     TDSEDHTVQAVDIRIVKSVTPAGLAVGQISTWSLAIATSEYRYFEDVEVSDTLGDGYC
                     PLGPVNYEHTPPPATAECNPTGNNPSV"
BASE COUNT          332 a          580 c          482 g          240 t
ORIGIN      
        1 gcgctttcgt cggacgccca tgagccggac cagatcggct tccggtacgg ggaagggatc
       61 gacttcctga acgaacacgg gatcaccgag atcgcgactt tccgcgaccg ggttcggacg
      121 acggcaccgc tgggtaaccc taaatagagg cgttcaggaa acggattgtt cagaaaaaca
      181 gatcagggac ggaacactgg cgaaatgacg acggggaatc agaagacacg gggaatactg
      241 atcgcgctgg ctctcctgct cggcgccctg atgatgttcg gtccggcccg ggccgaggcc
      301 gccggctacc cgaacatctc gatgtccaag accaccagcg gatccgttct gctcgggggt
      361 aactcgaacg tcacccttac cgcctcgaac ccgaacggcc agccgaccgg ctacaacctc
      421 agctttcggg acgttctccc gccggggatc agttacgtcc ccggttcctc accgtccagc
      481 accggcgagc cacaggtgat cgcgaacgcc cccgctgccg ggcagaccac gctgatctgg
      541 tcaaacgtcg ccgatctcac ccccaattcg accttcacct tcacttttca ggtcaccaac
      601 agcccggcgg tcttcgccat cggcgacacc taccagaaca cggccggcag ctatctgaac
      661 tgtgacccgc gttacgtgcc gaagttcaac gcccagggcc agccgaccca gagcggcgga
      721 aacgccacct gttcaggcct gcctcaggag acttcgtaca cgggctcggc gaccgcttcg
      781 gccaacacgc tgatcaaggc gatcgagatc gacaagtccg agccctcccc ggaggggcag
      841 ctgctccggg gtctgcacga ccaccagacc gtctacacgc tgaccgtcac caacaacgag
      901 gtcgatgccg acgacaacgt gaaggtcgag gattacctgc cggccggcct cgagttcctc
      961 ggttgtggca cccaggacaa cacgaccgac gcgccgacca acccgggcag cgcggaggag
     1021 taccccggct cgggcgcgat caacccgggc aacgctcctg ccgcgccgga ttgcgtggtc
     1081 cccgacaccg tcgaaacggt gctcaacccc tcaggcttgc cggccggggt ctacacgcat
     1141 gtggtctgga acaacctcgg caacctgggg ccttccggcc agctccggat ccagtacgtg
     1201 gcggcagtgc cgatccggca gaacaccatg acctggacca acggtgttcc tccggtaacc
     1261 gggacgcagg gttccaacct cgacaacaat tcgggtcccg aaaccaccca cctccagggg
     1321 ctgaccaacc acgcgaaggt gacctccgac catcagggcc cgggcggccc gaccgaggtc
     1381 actgactccg aggaccacac cgttcaggcg gttgacatca ggatcgtcaa gtcggtcacg
     1441 ccggccggtc tggcggtcgg ccagatcagc acctggtcgc tggcgatcgc gaccagcgag
     1501 taccgctact tcgaggatgt cgaggtcagc gacacgctcg gcgacggcta ctgcccgctg
     1561 ggcccggtca actacgagca caccccgccg ccggcaaccg ccgagtgcaa cccgaccggc
     1621 aacaacccgt cggt
//
LOCUS       DAOTIP010000543         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_89410_length_1628_cov_3.205976, whole genome shotgun sequence.
ACCESSION   DAOTIP010000543 DAOTIP010000000
VERSION     DAOTIP010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89410_length_1628_cov_3.205976"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..580)
                     /locus_tag="P5138_09620"
     CDS             complement(119..580)
                     /locus_tag="P5138_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60876.1"
                     /translation="MAAELSLVLILLCVIVVPIAAIAFARSGKGYEEIGKGRFAVDFD
                     ESSEAEQHEELRQLIEAKAYRQARRGETPVDVDSEMERLLGGRLPGEDDDPAAPTTEA
                     DPAAGTDDPELAGIREEIRQVVIAKNERRERQGEPLLDVEAEVERMLAEFG"
     gene            741..>1628
                     /locus_tag="P5138_09625"
     CDS             741..>1628
                     /locus_tag="P5138_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60877.1"
                     /translation="MVDFTLSDEQKDLREMAHNFAEKEMRSVAFEYDKDGTWPAEVIQ
                     KAWEVGLMNTHVDEAYGGPGLDYLSGCLIEEEFGWGCSGIGTSLACNGLASAPVSLGG
                     SEETKAKYLGMLCEEPKLASFCLTEPDAGSDVAGMKTRAVKKGDKYIINGSKCFITNG
                     AHADWFTVYAKTDPDAGSRGITAFVVDKQDGITVDKKEDKMGQRASNTVTLTFDDVEV
                     PAENMLGEENKGFKLAMMTLDRTRPGVAAMATGIARSGFDAAVQYSKERQQFGVPIAM
                     HQAIQFLIADMAVKVDAARL"
BASE COUNT          295 a          569 c          491 g          273 t
ORIGIN      
        1 ctggaccacc agctcctcga gttcgtagcg ggatgtggag tcatccatgc gcccatacta
       61 accacgacct gccgccggat cggaagattc accacacgat gccggccgtc gcgtcccgct
      121 agccgaactc ggccagcatc cgctcgactt cggcctcgac gtcgagcagc ggttctccct
      181 ggcgttcccg cctttcgttc ttcgcgatca ccacctgccg gatctcctcg cgtatcccgg
      241 ccagctcggg atcatcggtc ccggcggccg ggtcggcttc ggtggtcggt gccgccggat
      301 catcgtcctc gccgggcagg cgacccccca gcagccgttc catctcgctg tccacgtcca
      361 ccggggtctc gccccgcctc gcctggcgat aggccttggc ctcgatcagc tggcgcagtt
      421 cctcgtgctg ctcggcctcg ctgctctcgt cgaaatccac cgcgaaacgg cccttgccga
      481 tctcctcgta tcccttgccg gagcgggcaa aagcgatcgc ggcgatcggg accacgatca
      541 cgcagagcag gatcaggacc agggaaagct ccgcagccat gtcccaaggc tattgcctct
      601 cccgctgggc ggccccatcc ggtacccttc cctgagagtt attgactgac tagtcaatcg
      661 ggagccgtcc gggcccctcg ccccggcccc gcatcgagca aatcgaacgg ttccaagcaa
      721 ccgaaggagt ttgaacacaa gtggttgact tcacgcttag cgacgagcag aaggacctgc
      781 gcgagatggc gcacaacttc gccgagaagg agatgcggag cgtcgccttc gaatacgaca
      841 aggacggcac ctggccggcc gaagtgatcc agaaggcctg ggaagtcggc ctgatgaaca
      901 cgcacgtcga tgaggcatac ggcggaccgg gcctggacta cctgtccggc tgcctgatcg
      961 aggaagagtt cggctggggc tgctccggca tcggcacctc gctcgcctgt aacggcctcg
     1021 cctccgcccc cgtttccctc ggcggctccg aggagaccaa ggccaagtac ctcggcatgc
     1081 tctgcgaaga gccgaagctt gcctccttct gcctgaccga gccggacgct ggctccgacg
     1141 tcgccggcat gaagacccgg gccgtgaaga agggtgacaa gtacatcatc aacggctcca
     1201 agtgcttcat caccaacggc gcgcacgccg actggttcac ggtctacgcc aagaccgatc
     1261 cggacgccgg cagccgcggc atcactgcct tcgtggtcga caagcaggac ggcatcacgg
     1321 tcgacaagaa ggaagacaag atgggtcagc gggcctcgaa taccgtgacc ttgacctttg
     1381 acgacgtcga ggtcccggcc gagaacatgc tcggcgagga gaacaagggc ttcaagctcg
     1441 ccatgatgac cctcgaccgc acccgtcccg gtgtcgccgc catggccacc ggcatcgccc
     1501 gctccggctt cgacgccgcg gtccagtact cgaaggagcg ccagcagttc ggtgtgccga
     1561 tcgcgatgca ccaggcgatc cagttcctga tcgctgacat ggcggtcaag gtggacgcgg
     1621 cccgtctg
//
LOCUS       DAOTIP010000544         1625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_89667_length_1625_cov_2.640764, whole genome shotgun sequence.
ACCESSION   DAOTIP010000544 DAOTIP010000000
VERSION     DAOTIP010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89667_length_1625_cov_2.640764"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="P5138_09630"
     CDS             <1..1347
                     /locus_tag="P5138_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV60878.1"
                     /translation="HGVIRIGGDRGGWKAWQNNQIMHENLAVWLERAGYRTMHFGKFM
                     NNYGGPDSPAETIVPPGWDDWITDATDNSTREFYGYQQNIDGQITPRLGWPYYDQQGG
                     RDPEGCPWLGVEVCNYHTDSMSMQAEDAIRNSGNKPFYIQIDYHTPHGDSRPPIGPEP
                     APRHYDTALKTPGPRPPGFNERDMSDKPKFLQQAIADSPLGGQEIKQLATEYRKSVEA
                     LRSVDDGVGGIIKALRETGRLSNTYVIFTSDNGFFTGQHRITRGKLLPYDPALRVPFV
                     IRGPGIKPGTKSYEPVANQDVAPTIVRLAGARAALTIDGRSMKRFWLDPKRRSRRPIL
                     LSSYTSITRLIPGDYPDEPADATAGNGDASASVKSPNQNYVGIRLGPYKYIRYENGEK
                     ELYVLKDDPAELENRASDWRYWRIVRYLDRQLEVLRGCKGQTCRNWAPRWPQPPGD"
     gene            complement(1392..>1625)
                     /locus_tag="P5138_09635"
     CDS             complement(1392..>1625)
                     /locus_tag="P5138_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60879.1"
                     /translation="MFITGSWNGTVPAAPRKAPIFAIAVNGRVRATARPFDFDGAARW
                     GALVNPASIHQGKNRIGIYRLAGKRLIPLGGNF"
BASE COUNT          319 a          551 c          531 g          224 t
ORIGIN      
        1 cacggcgtga tccggatcgg cggggaccgg ggcgggtgga aggcctggca gaacaaccag
       61 atcatgcacg agaacctggc tgtctggctc gagcgggccg gctaccggac catgcacttc
      121 ggaaagttca tgaacaacta cggcggcccc gactcgcccg ccgaaacgat cgtgccgccc
      181 ggctgggacg actggatcac cgacgccacc gacaattcga cccgcgagtt ctacgggtac
      241 cagcagaaca tcgacggcca gatcacaccc cggctgggct ggccctacta cgaccagcag
      301 ggcggccgcg atccggaagg ctgcccctgg ctcggcgtcg aggtctgcaa ctaccacacc
      361 gattcgatgt cgatgcaggc cgaggatgcg atccgcaact cgggcaacaa gcccttctac
      421 attcagatcg actaccacac gccccacggc gattccaggc cgccgatcgg gcccgaaccg
      481 gcgccgaggc attacgacac cgccctgaag accccggggc cgcggccacc gggcttcaac
      541 gagcgcgaca tgagcgacaa gccgaaattc ctgcagcagg ccattgccga ctcgccccta
      601 ggcgggcagg aaatcaagca actcgccacc gaataccgca agtcggtcga ggccctgcgt
      661 tcggtcgatg acggtgtcgg cgggatcatc aaggcgctgc gggagaccgg ccgcctctcc
      721 aatacctacg tgatcttcac ctcggacaac ggtttcttca ccggccagca ccggatcacc
      781 cgtggcaagc tgctgcccta cgacccggcc ctgcgggtgc cgttcgtgat ccgggggccg
      841 ggcatcaaac ccgggacgaa gagttacgaa ccggtggcaa accaggacgt ggccccgacc
      901 atcgtcaggc tggccggcgc ccgggcggcg ctgaccatcg acgggcgctc gatgaagcgg
      961 ttctggctcg accccaagcg ccggtccagg cgaccgatcc tgctctccag ctacacgtcg
     1021 atcacgcgcc tgatccccgg cgactatccg gacgagccgg ctgatgccac cgctgggaac
     1081 ggggacgcca gtgcctcggt caagagcccg aaccagaact acgtcgggat caggctcggc
     1141 ccctacaagt acatccgtta cgagaacggg gagaaggagc tctacgtcct gaaggacgat
     1201 ccggccgaac tcgagaaccg ggcctcggac tggcgctact ggcggatcgt caggtacctc
     1261 gacagacagc tcgaggtcct gaggggctgc aaggggcaga cctgtcgcaa ctgggcaccc
     1321 agatggcccc agcccccggg ggactgagga gccgggcggc cgaggagcct ggcgactgag
     1381 aagccgggcg actagaagtt gccgccgagg gggatcagcc gctttccggc caggcggtag
     1441 atgccgatcc ggttcttacc ctgatggatc gaggccggat tgacgagggc accccagcgg
     1501 gctgcgccgt cgaagtcaaa gggccgggcg gtcgcccgga cccgtccgtt gacggcgatc
     1561 gcaaagatcg gtgccttcct cggcgcggcc gggacggtgc cgttccagga tcccgtgatg
     1621 aacat
//
LOCUS       DAOTIP010000545         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_90182_length_1618_cov_3.193218, whole genome shotgun sequence.
ACCESSION   DAOTIP010000545 DAOTIP010000000
VERSION     DAOTIP010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90182_length_1618_cov_3.193218"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="P5138_09640"
     CDS             <1..882
                     /locus_tag="P5138_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60880.1"
                     /translation="ALMTEYQAFGVRHFLRLLDAEGASELRRRVIPRLDGTETEKGAW
                     SDTDELVMDPGQQGVLTYRTLVLRRNPRQSRPPSPYKRVWQGKYYEVWQRDPDYDAAN
                     LIVHKPLGGDFQPAAVPNCAVVQSVATEARNGNGKVVAAERQPNAVADLTSYPPDWVP
                     DPANKTLTPLSDGTATGSITVPKTGRYQVWVGGSARGRVSVEIGGQDAGSALGLLNNN
                     AQFISLADLDFEAGSLPVTLSYEKGGNLRPATGGYPFGIGPVVLEPVAEPEVVTVTPA
                     DAQKLCGRSLDWIEAVR"
     gene            1030..1194
                     /gene="rpmG"
                     /locus_tag="P5138_09645"
     CDS             1030..1194
                     /gene="rpmG"
                     /locus_tag="P5138_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRV60881.1"
                     /translation="MARGDVRIAVTLACENCKRRNYQTNKSKRNTPDRIEMRKYCRWC
                     GKHQPHKETR"
     gene            1194..>1618
                     /locus_tag="P5138_09650"
     CDS             1194..>1618
                     /locus_tag="P5138_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRV60882.1"
                     /translation="MAKSRAQRKAERRAREAAAQGGVESDSQAQHDTQVPKSGDIAEI
                     EAVEAGIAAGADEKDLETPDAPKHGISSKEEKRKARQDEKRKKAEQKKHEEQQLAKRQ
                     KQAQTERKRGGVIAFLSSCIAELKRVQWPDRDTLIQATAV"
BASE COUNT          326 a          519 c          536 g          237 t
ORIGIN      
        1 gccctgatga cggagtacca ggccttcggg gtccgccatt tcctacgtct tctcgatgcc
       61 gaaggggctt cggagctgcg ccgccgggtg attccgcgac tcgacgggac cgagaccgag
      121 aagggggcct ggtcggacac cgacgagctg gtgatggatc cgggccagca gggggtcctc
      181 acctaccgga ccctggttct gcgccgcaat ccgaggcaaa gccggccacc ttccccctac
      241 aagcgggtgt ggcagggcaa gtactacgag gtctggcagc gcgacccaga ctacgacgcc
      301 gccaacctga tcgtccacaa gccgctcggg ggtgatttcc agcccgccgc ggtgccgaac
      361 tgcgctgtcg tccagagcgt cgcgaccgag gcccgcaacg gcaacggcaa ggtggtggcg
      421 gcggaacggc agccgaatgc ggttgccgac ctgaccagct atcccccgga ctgggttccg
      481 gacccggcca acaagacgct caccccgctc tctgacggta ccgccaccgg gagcatcacc
      541 gtgccgaaga ccggcaggta tcaggtctgg gtcggcggat cggcccgggg ccgggtctcg
      601 gtcgagatcg gcggccagga cgcgggctcg gcgttggggc tgctgaacaa caacgcccag
      661 ttcatcagcc tcgccgatct cgacttcgag gccgggtctt tgccggtcac gctgagctac
      721 gaaaaaggcg gcaatctgcg ccccgcgacc gggggctatc cgttcgggat cggaccggtc
      781 gtgctcgaac cggtggccga acccgaggtt gtgaccgtta cgccggccga cgcgcaaaaa
      841 ctttgcggcc ggtccctgga ctggatcgaa gcggtccgct agccacgatt cgggctctcg
      901 gttcccagtt agcagtccaa ccggacgcga ggacccgcgg ctcgctctga accgttattc
      961 ttcgtagacg cgcctctgcc cccgggctga ggctttactt tgtcagcact gcctgagtag
     1021 cgaggaaata tggcacgcgg agatgtccgc atcgcggtga cgctcgcatg tgagaactgc
     1081 aagcgtcgta actaccaaac gaataaatcg aagcgcaata ctcccgatcg aatcgagatg
     1141 cggaagtact gtcgctggtg cggcaagcac cagccccaca aggaaacacg ctagtggcga
     1201 agtcccgggc acagcggaag gccgagcgcc gcgcgcgcga ggctgcggca cagggcggtg
     1261 ttgagtctga ttcccaggct cagcacgaca cccaggtgcc caagtccgga gacatcgccg
     1321 agatcgaagc cgtcgaagcc ggcattgcag ccggtgccga cgagaaggat ctcgaaactc
     1381 ccgatgcccc caagcacggc atcagttcga aagaagagaa gcggaaggca cgccaggacg
     1441 agaagcgcaa gaaggccgag cagaagaagc acgaagagca gcagctcgcc aagcgacaga
     1501 agcaggccca gaccgagcgc aagcgcggcg gggtcatcgc tttcctgtca tcctgcattg
     1561 ccgagctgaa gcgggtccag tggccggatc gggataccct gatccaggcg acggcggt
//
LOCUS       DAOTIP010000546         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_90641_length_1612_cov_3.551702, whole genome shotgun sequence.
ACCESSION   DAOTIP010000546 DAOTIP010000000
VERSION     DAOTIP010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90641_length_1612_cov_3.551702"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="P5138_09655"
     CDS             <1..952
                     /locus_tag="P5138_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60883.1"
                     /translation="SACASTSGTPETGIANYDMANAPAGGYTMMGSPTVIAKVTQADS
                     TSSQLAFRLVDVSADGTTKSLVARTAYRSPGDGFQVIQLNANGWTVAEGHHLRLEVLP
                     FDGGNANPAAPLSNYTRPSNDQQPATIEDLQLRIPVQEQPGALNGLVEAPAKRVLPDY
                     AGVELAPGNEAIGSQTLEQYRKANYPVIGKIKIVKATVKGKTLTARVKCAGSYDSCAK
                     ATVKFKGAPKKGKGKGVLLASKSGVTAGAGKTVSVKFKMTSKARKLFRDSKKRKVIRK
                     HGRKRVKIVKVKGLKSLRTKFMINGQAGGYLSVKRTGKVK"
     gene            1297..>1612
                     /locus_tag="P5138_09660"
     CDS             1297..>1612
                     /locus_tag="P5138_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60884.1"
                     /translation="MRKFAVALAALFMFVVVPQAHADITSIFGGKIPCTQQSQPDGRI
                     LCSGPGGSDSNAPANTVPSYDGTPIDVNVAFPNPEVYGDGPYPLAMYFHGFGGGKEGF
                     DGD"
BASE COUNT          325 a          543 c          500 g          244 t
ORIGIN      
        1 ttccgcttgc gcgtcgacct ccggaacccc ggagaccggc atcgccaact acgacatggc
       61 caacgcgccc gccgggggct acaccatgat gggctcgccg acggtcatcg ccaaggtcac
      121 ccaggccgac agcaccagtt cacagctggc cttcaggctg gttgacgtct cggccgacgg
      181 cacgaccaag agcctggtcg cccggaccgc ctaccgctca cccggtgacg gcttccaggt
      241 gatccagctc aacgcgaacg gctggacggt cgccgaaggt caccacctgc ggctggaagt
      301 cctgccgttc gacggaggca acgccaaccc ggcagctccg ctgtcgaact acacccgtcc
      361 gtcgaatgac cagcagccgg ccacgatcga ggacctccag ctgaggatcc cggtccagga
      421 acagccgggt gccctgaatg gcctggtcga agccccggcg aagcgggttc tgcccgacta
      481 cgcgggcgtc gaactcgccc cgggcaacga ggcgatcgga tcgcagacgc tggagcagta
      541 ccgcaaggcg aactacccgg tgatcggcaa gatcaagatc gtcaaggcga cggttaaggg
      601 caagacgctc accgcccggg tcaagtgcgc cggctcgtac gattcctgcg ccaaggcgac
      661 cgtgaagttc aagggcgccc cgaagaaggg caagggcaag ggcgtcctgc tggccagcaa
      721 gtccggcgtg accgccgggg ccggcaagac cgtctcggtc aagttcaaga tgacctccaa
      781 ggcccgcaag ctgttccgcg acagcaagaa gcgcaaggtg atccgcaagc acggccgcaa
      841 gagggtcaag atcgtcaagg tcaaggggct caagtcgctc cggaccaagt tcatgatcaa
      901 cggccaggca ggcggttacc tcagcgtgaa gcggaccggc aaggttaagt aacaggaagt
      961 cgtccggtct tcgccggacg acaacaggaa cccgcccctc ccaatcacat tcactgatgg
     1021 ttgggagggg ctccgcctgc gtcacttccc ggcaagctga cttttcgcgt tcccccgaag
     1081 gtcttttccg ggtatgcccg caccggacct catagccggc cgtcggccaa tcccagtccc
     1141 tgccgttaag ccaccgccga tccggcatcg gggcgttccg tgaacgggtg gtaatctgaa
     1201 taggtcaagg gggcgagccg attggcccgt gcccgtgttc tgaaagggaa gccgcaggga
     1261 gcggcaccaa cgtcaatagg gagaaacagg gaccaaatgc gcaagttcgc cgtagccctc
     1321 gctgcacttt tcatgttcgt cgtcgtgccc caggcacacg ccgacatcac ctcgatcttc
     1381 gggggcaaga tcccttgtac gcagcagtcg cagccggacg gccgcattct ttgctccggc
     1441 ccgggcggtt ccgactcgaa cgcaccggca aacacggtcc cgagctacga cggcacgccg
     1501 atcgacgtca acgtcgcctt ccccaacccg gaggtctacg gtgacggtcc ctacccgctg
     1561 gccatgtact tccacggttt cggaggcggc aaggaaggct tcgacggtga cc
//
LOCUS       DAOTIP010000547         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_90653_length_1612_cov_3.103404, whole genome shotgun sequence.
ACCESSION   DAOTIP010000547 DAOTIP010000000
VERSION     DAOTIP010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90653_length_1612_cov_3.103404"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="P5138_09665"
     CDS             <1..705
                     /locus_tag="P5138_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRV60885.1"
                     /translation="TMLDIEVVTADQLEASGGELIAREGEERKRIDVLYNRSNTDRIT
                     RSGNDLTPLGALLIEPLRSGKLACVNSFGAGVADDKAVHCYTGEIIRFYLGEEPLLDI
                     PESFDLGDPDHAEEVLGRLDELVIKPRWALGGQDIVIGPKASARELERARQAVRSNPG
                     HWVAQEMIGLSTHPTLIGDRLEPRHIDLRPYVITSAGEQRVTPLALTRFAKAADNLVV
                     SSTQGGGAKDTWILAD"
     gene            687..1511
                     /locus_tag="P5138_09670"
     CDS             687..1511
                     /locus_tag="P5138_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRV60886.1"
                     /translation="MDTGGLTLRGLTRAPETRRPLIGVTTSELRKAETVSPTPEGEPP
                     RHEMALGLTYMKAVEVAGGVPVVIPPMEVGLIGALLEHLDGVCLSGGPDIHPDHYGEE
                     PHHHLGPTAPDLDHFELEIARKADAMMMPVLAICRGMQALNISRGGTLLQHIPDRWLD
                     IEHRQKMPGQTAAHRVDLDPKSHVSLIVGSPVIAVNSFHHQAVEKVGEGLVATGWAPD
                     GATEAIEDPNREFMIGVQWHAELMVDQPEQYKLFRQFVAAAEDFSVARLSEATSLR"
BASE COUNT          318 a          520 c          522 g          252 t
ORIGIN      
        1 acaatgcttg acatcgaagt ggtcaccgcc gatcagctcg aggcttcagg cggcgagctg
       61 atcgcccgcg agggagaaga gcgcaagcgg atcgacgtgc tctacaaccg cagcaacacc
      121 gaccggatca cccgatcggg aaacgacctg accccgctcg gcgccctatt gatcgaaccg
      181 ctccgctcgg gcaagctggc ctgcgtgaac tcgttcggcg ccggcgtcgc cgacgacaag
      241 gcggttcact gctacaccgg ggagataatc cgcttctacc tcggtgagga accgctcctg
      301 gatattccgg aatccttcga cctcggggac ccggaccatg cggaggaggt gctggggcga
      361 ctcgacgagc tggtgatcaa gcctcgatgg gccctcggag gccaggacat cgtgatcggt
      421 ccgaaagcct cggccaggga actcgaacgg gcccgccagg cagtccgcag caaccccggt
      481 cactgggtgg cccaggaaat gatcggactg tccacccacc ccaccctgat cggcgaccgt
      541 ctggaacccc gtcatatcga tttgagaccg tatgtgatca cttcagcagg agagcagagg
      601 gtgacaccgc tggcgttgac cagattcgcc aaggcagcag acaaccttgt tgtcagcagc
      661 acccagggag gaggagcgaa ggacacatgg atactggcgg actgacgctg cggggtctga
      721 cccgcgcccc ggaaaccagg cgcccgctga tcggggtcac cacctcggag ttgcgcaagg
      781 ccgaaacggt ctcgccgacg ccggaggggg agccgccgcg ccacgagatg gcgctcgggc
      841 tcacctacat gaaggccgtc gaagtcgccg gcggcgttcc ggtagtgatc ccgccgatgg
      901 aagtcgggct gatcggcgcc ctgctcgaac acctcgacgg ggtctgcctc tcgggtggcc
      961 cagacatcca tcccgatcat tacggggagg aaccacacca tcacctcggg ccgaccgcac
     1021 ccgacctcga tcatttcgaa ctcgagatcg cccgcaaggc ggacgcgatg atgatgccgg
     1081 ttctcgccat ctgccgcgga atgcaggcgc tcaacatcag ccgcggcggg accctgctgc
     1141 agcacatacc ggatcgctgg ctggatatcg agcaccgtca gaagatgccc ggacagaccg
     1201 ccgcgcaccg ggttgacctg gaccccaagt cccatgtttc gctgatcgtc ggttcgccgg
     1261 tgatcgcagt caactccttt caccaccagg ccgtcgagaa ggtcggtgaa ggcctggtcg
     1321 cgaccggatg ggcccctgac ggggcgaccg aagcgatcga ggatccgaac cgcgagttca
     1381 tgatcggcgt ccagtggcat gccgagctga tggtcgacca gcccgagcag tacaagctgt
     1441 tccgccagtt cgtggccgca gcggaggact tctcggttgc cagactctcg gaggcgacct
     1501 cattacgctg aacggcgaaa gcggagcgct gccggattgg gctgtctggc cgaagcagcc
     1561 tcatgccctg ggcgggttca ccatcggggt ggaagaagag gtgatgctga tc
//
LOCUS       DAOTIP010000548         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_90661_length_1612_cov_2.818882, whole genome shotgun sequence.
ACCESSION   DAOTIP010000548 DAOTIP010000000
VERSION     DAOTIP010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90661_length_1612_cov_2.818882"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="P5138_09675"
     CDS             <1..1447
                     /locus_tag="P5138_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRV60887.1"
                     /translation="PLALIETLVRFPTGRIESNVDRWVLRLVIVVLSIVTLSYFFATS
                     SMLGQEQAPRNLLMIVESQTVFTILQLFVNAFAIALILALIRSFTNKYSSASPPMRRL
                     IGPVLWTSTLVLAGLGISSVFSIAGLDSAENIAGIFTAIMATALPFAFLVGLARSRLV
                     RGEALGRMLERVAHENDPVKMQEAVAEALGDPSVELAFWLPGTESYVDARGLPIDEPE
                     TDSGRAASEVTMEGRKVALIIHDDALLEEPEKIEAVSRGAALAFENARLEAEVRANVA
                     ELRASRTRIVEAADAARREIERNLHDGAQQNLVSLALKLQMARARVESDPRGAIEIID
                     GASAELEQTLAELRELARGIHPAVLSDRGLDAALLALAHRAPLPVEVGEMPEERLPEQ
                     VEAAAYYVVAESLTNVARYSEASRAKVDITRNNGTVRIRIADDGVGGADPTRGSGLKG
                     LADRIAALDGHFMLASPDGLGTIVEAEIPCG"
BASE COUNT          255 a          532 c          553 g          272 t
ORIGIN      
        1 cccgctcgcc ctgatcgaga ccctggtccg cttcccgacc ggccggatcg agtcgaacgt
       61 ggaccgctgg gtcctgaggc tggtcatcgt ggtcctctcg atcgtcaccc tgagctactt
      121 cttcgcgact tcgtcgatgc tcgggcagga gcaggctccc cggaatcttc tgatgatcgt
      181 ggaatcgcag acggtcttca cgattcttca gttgttcgtg aacgcatttg cgatcgccct
      241 catcctggcg ctgattcgca gcttcacaaa caagtattcg agcgcgtccc cgccgatgcg
      301 tcgtctgatc ggacccgtcc tctggacctc gaccctggtc ctggccgggc tgggtatttc
      361 gtcggtcttc tcgatcgccg gactcgattc cgccgagaac atcgcaggga tcttcaccgc
      421 gatcatggcc accgccctgc cgttcgcctt cctggtcggc ctggcccgaa gccgtctggt
      481 gcggggagag gcactcgggc ggatgctcga gcgggtcgcg cacgagaacg atccggtcaa
      541 gatgcaggag gcggtggccg aagcgctggg tgacccgtcc gtcgagcttg ccttctggct
      601 gccgggcaca gagagttacg tcgatgcccg tggcctgccg atcgacgagc cggaaaccga
      661 ctccggccgc gccgccagcg aggtgaccat ggagggacgg aaggtcgccc tgatcatcca
      721 tgacgacgcc ctgctcgaag agccggagaa gatcgaggcg gtcagccgcg gtgccgccct
      781 cgcgttcgag aacgcgcggc tcgaggccga agtgcgggcc aacgtcgcgg aactgcgtgc
      841 gtccaggacc cgtatcgtcg aggccgccga tgcggcccgc cgcgagatcg agcggaacct
      901 ccacgacggg gcccagcaga atctggtttc gctcgcgctg aaactgcaga tggcccgggc
      961 cagggtcgaa tccgacccgc gcggcgcgat cgagatcatc gacggagcca gcgcggagct
     1021 cgagcagact ctggccgaac tgcgtgagct cgctcgcggc atccaccctg ccgtcctttc
     1081 cgaccggggc ctcgatgccg ccttgctggc gctggcccac cgggcgccct tgccggtcga
     1141 agtcggtgaa atgcccgagg agcgcctgcc ggaacaggtc gaggcggccg cctactacgt
     1201 cgtggccgaa tcgctgacca acgtcgcccg ttactccgag gccagccggg ccaaggtgga
     1261 tatcacccgg aacaacggca ccgtccggat ccggatcgcc gatgacgggg tcggtggcgc
     1321 cgacccgacc cggggctcag gcctgaaggg cctggccgac cggatcgccg cgctggatgg
     1381 ccatttcatg ctggccagcc cggacggtct gggcacgatc gtggaggcgg agatcccatg
     1441 cgggtgagga aaggcggtat cccatgcggg tagtggtcgc tgacgattcg gtcctcttgc
     1501 gcgaaggcgt ggtgcgggtg ctggaagacg acggcttcga ggtggtcggc caggccggcg
     1561 acgccgagga cctgttgcga aaggtcggcg cccacaagcc cgatgtcgcg gt
//
LOCUS       DAOTIP010000549         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_90953_length_1608_cov_4.092724, whole genome shotgun sequence.
ACCESSION   DAOTIP010000549 DAOTIP010000000
VERSION     DAOTIP010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90953_length_1608_cov_4.092724"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="P5138_09680"
     CDS             complement(<1..1219)
                     /locus_tag="P5138_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60888.1"
                     /translation="MNRPRAAILAAITGLALLALTLPATSAADDAASRNENTAILVLI
                     PQDTETPPSYSEEIADGFADLPQLSVGLVSATQGSYRREQALLDISQGTRVSRSTYDP
                     SEVPPVGLARMVGGGGVVKGWDAILDRAKTAPQTIEPGLLASSIPGGAAYVGDATKLQ
                     ETVIPATSRLGFVSSLSLADTTDTARRAIRESRRYRLVVVATSPGRNGLRELRQILHT
                     RRPGQLVIATQTPPLAGILPLLPIAAAGLGENAGDPVDPEGKGLRSDTTNLPDLVAGI
                     DIAPTILDHLGLTIPDAMTGKPFRAEGERDPEALTPFRDRLDVLGPRRNAALAFVFVG
                     WLILYLAAGAILGRERAARRVRRIGGLSILWIPTVILVPAALGNPSAEVEYTLIAGLA
                     LLFGFLSDRFLDWP"
     gene            1284..1544
                     /locus_tag="P5138_09685"
     CDS             1284..1544
                     /locus_tag="P5138_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60889.1"
                     /translation="MSNEPEQSPSARIGALAQELMEQVERDTPGARLDQLVISAVLSE
                     DAGIDEQTMHVQLVSDSSDPLSVVGLLTVALELAKGQLTGGE"
BASE COUNT          267 a          520 c          575 g          246 t
ORIGIN      
        1 agggccagtc gaggaaccgg tccgaaagga acccgaacaa cagggcgaga cccgcgatca
       61 gggtgtactc gacctcggcc gacgggttgc cgagggcggc cgggacgagg atcacggtcg
      121 ggatccagag gatcgacaga ccgccgatcc tccgcacccg ccgcgcggcc cgctcacgcc
      181 ccagtattgc cccggcggcc agatagagga tcagccagcc gacgaagacg aaagccaggg
      241 cggcgttccg ccgcggtccg agaacgtcaa gccggtcgcg gaagggtgtc agcgcttccg
      301 ggtccctctc gccttcagcc cggaaaggct tgccggtcat cgcatcggga atcgtcaggc
      361 cgaggtggtc gaggatggtc ggggcgatgt cgattccggc aaccaggtcc ggcaggttgg
      421 ttgtgtcaga cctgagcccc ttgccctcgg ggtcgaccgg atcgcccgcg ttctcgccca
      481 gcccggccgc ggcgatcggc agcagcggca ggatcccggc cagaggcggg gtctgggtgg
      541 cgatcacgag ctggcccggc cggcgcgtgt ggaggatctg cctcagttcg cggaggccgt
      601 tgcggccggg ggaggtggcg accacgacca gccggtaacg gcgcgactcc ctgatcgcgc
      661 gccgggcggt gtcggtggtg tcggccagcg aaagggatga gacgaacccg agccggctgg
      721 tcgccgggat gaccgtttcc tgcagcttgg tcgcgtctcc cacataggcg gccccaccgg
      781 ggatggagct ggcgagcaga ccgggctcga tcgtctgggg agcggtcttt gcccggtcca
      841 ggatcgcgtc ccatcccttg actacacccc ctccgccgac catccgggcc aggcccaccg
      901 gcggcacctc ggaagggtca taggtcgaac gggaaacccg ggtgccctgg cttatgtcca
      961 gcagggcctg ttcccgccgg taggagccct gggtcgccga aacgagaccg accgatagct
     1021 gaggtaagtc ggcgaatcca tctgctattt cctcgctgta tgagggcgga gtttccgtgt
     1081 cctgggggat caggacgagg atcgccgtgt tctcgttcct gctcgcggca tcgtccgctg
     1141 cgctggtggc agggagagtc agggccagca gggccaagcc ggtgatcgcc gcgagaattg
     1201 cggcgcgggg gcgattcacc ggctcagtgt ttcagagctt tgcgacgacc ggcccgatcg
     1261 ggtgcgggcg gtagggtgac tgcatgagca atgaaccgga gcagtcgccg tcggcaagaa
     1321 tcggagcact ggcccaggag ttgatggaac aggtcgagcg tgacacgccc ggcgcccgac
     1381 tcgaccagct ggtcatctcg gcggtactca gcgaggatgc cggaatcgac gagcagacga
     1441 tgcacgtgca gcttgtctcg gattcctccg atcccctctc ggtcgtcggc ctcctgaccg
     1501 tcgcccttga actggccaag ggtcagctaa ccggcggcga gtaacccgag cggcccgggc
     1561 aacgcgccca ggaaccacca gccgcacgct gaccttcgtc ggtcgctc
//
LOCUS       DAOTIP010000550         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_91213_length_1605_cov_3.170968, whole genome shotgun sequence.
ACCESSION   DAOTIP010000550 DAOTIP010000000
VERSION     DAOTIP010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91213_length_1605_cov_3.170968"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..923)
                     /locus_tag="P5138_09690"
     CDS             complement(102..923)
                     /locus_tag="P5138_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60890.1"
                     /translation="MKTPGGAVSIGIVAILGLALMLPAGVEAGKKKHGVPTLLTYKAM
                     VHVAGGVTLTSQHDTFHACQPGQRWTLEEKTDVNIRGNMLIETYKKQQVRSTSVADPG
                     SVNNDHELASYEESNYCLPDEPVELDKPDCNGFTVKGVAGLQPDARPNGPKRVSIGIG
                     RKGEGIQDLSCIWGLPSKATPTGSQVGSLSSPWTSIVLPLDIRVGQLHTLGVKKSLIR
                     TIRVGGTCARPVVYRGKKFPTDTTDMEDGDCVIDGDFAVTIKRLNRKSKQGVPVG"
     gene            complement(920..>1605)
                     /locus_tag="P5138_09695"
     CDS             complement(920..>1605)
                     /locus_tag="P5138_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60891.1"
                     /translation="SNGPDIGFLSVPSGYDVNRTDVAANGKLFAIELDYNTIELYPID
                     RVPDTGQSSTVGSGGCVLPTQGDANHVSLSQDGKSVAWEDDRGVLVAGVPDFGGADPC
                     HLTRAPVVIASNGGYPSIGAATIPGSGGSLVPKPSVPTTVKAAKLKTGIWITVKVGKA
                     GPVKITGKVGKTVVARGSARAKKAGKVKVKLKAVGAFRNRPQRLKGRTLVIKVTSAGK
                     SKTVRRVLK"
BASE COUNT          235 a          542 c          497 g          331 t
ORIGIN      
        1 tcttctcgac cggatcgtgt tcgtcgagca gggcccggat ccggtcggcc agttcctcgt
       61 cgtagggcac cgtcaggtcc aggttcggcg aacgccccgg tctagccgac cggaacaccc
      121 tgcttcgact tgcggttcag cctcttgatc gtgaccgcga agtcgccgtc gatcacgcag
      181 tcgccgtctt ccatatcggt ggtgtcggtc gggaatttct tgccgcggta gacaaccggt
      241 ctggcgcagg ttccgccaac ccggatcgtc cggatcaggc tcttcttcac cccgagggtg
      301 tgaagctggc cgaccctgat gtccaggggc aggacgatgc tggtccacgg gctgctcaac
      361 gaaccgacct gcgaaccggt cggggtggct ttgctgggca ggccccagat gcagctcagg
      421 tcctgtatgc cttcgccctt gcgaccgatc ccgatcgaca cccgcttcgg gccattgggg
      481 cgcgcatccg gctgcagccc ggcaactccc ttgaccgtga aaccgttgca atccggcttg
      541 tcgagctcga ccggttcatc cggcaggcag tagttggact cttcgtagct ggccagttca
      601 tggtcgttgt tgacgctgcc tgggtccgcc accgaagtgc ttcggacctg ctgcttcttg
      661 taggtctcga tcagcatgtt gccgcggatg ttgacgtcgg tcttttcctc cagtgtccag
      721 cgctgaccgg gctgacaggc atgaaaggtg tcgtgctgac tggtcagggt gaccccgccg
      781 gcgacgtgaa ccatcgcctt gtaggtgagc agggtcggaa cgccgtgctt cttcttgccg
      841 gcctcgaccc cggccggcag cataagcgcc aatcccagaa tcgccacgat cccgatcgaa
      901 acggccccgc ccggtgtttt catttgagca ctctcctcac ggtcttgctc tttccggccg
      961 aagtgacctt gatgaccagg gtcctgccct tcagcctttg tggccgattg cggaaggccc
     1021 cgaccgcctt cagcttcacc ttgaccttgc cggccttctt cgcccgggcg ctgccccggg
     1081 cgaccacggt cttgccgacc ttgccggtga tcttcaccgg tccggccttg ccgaccttga
     1141 ccgtgatcca gattccggtc ttgagcttcg cggccttcac cgtcgtcggc acggacggct
     1201 tcggcaccag gctgccgcca ctgcccggaa tggtcgccgc gccgatcgac ggataaccgc
     1261 catttgacgc gatcacgacc ggggccctcg tcaggtggca ggggtcggcc ccgccgaaat
     1321 caggcacgcc ggccaccagg acgccgcggt cgtcctccca ggcgacactc ttgccgtcct
     1381 gcgaaaggga gacatggttg gcgtcaccct gggtcggcag cacgcagccg ccgctgccca
     1441 ctgtcgagct ctgcccggtg tcggggaccc ggtcgatcgg atagagctcg atcgtgttgt
     1501 agtcgagctc gatcgcgaac agcttgccgt tggctgccac atcggtccgg ttcacgtcgt
     1561 aacccgaggg aacgctgagg aagccgatgt ccgggccgtt gctga
//
LOCUS       DAOTIP010000551         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_91455_length_1602_cov_3.369748, whole genome shotgun sequence.
ACCESSION   DAOTIP010000551 DAOTIP010000000
VERSION     DAOTIP010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91455_length_1602_cov_3.369748"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="P5138_09700"
     CDS             <1..615
                     /locus_tag="P5138_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRV60892.1"
                     /translation="RGEEYWARPVPGFGDPKARILVHGLAPAANGGNRTGRPFTGDRS
                     GDWLFAAIHRAGLSNQPESTGRDDGLELRDTFVNAAVRCAPPENKPLPSERDNCAPWL
                     EGELELLTEVKVVIALGGFAWEAMLRTVRGLGGGIPKPKPKFGHETEVTLEAPAGADW
                     SGPERWVLLGCFHPSPLNTQTGRLSAAMLDDVFARARDLAAAGP"
     gene            770..1297
                     /locus_tag="P5138_09705"
     CDS             770..1297
                     /locus_tag="P5138_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60893.1"
                     /translation="MAAALCLFAVRPVIGDMLNSEPDPAPVVVAATHPATSAQVADAR
                     GLPGTGLAAGASASTTRKSKSGRNTRVIRTVYRRVKINSRITDQEILTEEGKGTFVGI
                     KCPKGSKAISGGVLSKYINLLVSSSAPNHPLTRKYTPNIWWLTVVNVNVDGNGGTLPW
                     LGVVNCLSPVKLGSG"
     gene            complement(1308..>1602)
                     /locus_tag="P5138_09710"
     CDS             complement(1308..>1602)
                     /locus_tag="P5138_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60894.1"
                     /translation="GWLAERVKDGEVRWVLASDQGTSPADGRVGSSELMTAVTQACEP
                     VQLESSGTDSTTTGASAGQQSGTASASSNGTDSTTDSNVIYDCAGKASALAAQ"
BASE COUNT          259 a          566 c          545 g          232 t
ORIGIN      
        1 cgcggcgagg agtactgggc ccggccggta cccgggttcg gcgatccgaa ggcccgcatc
       61 ctcgtgcacg gcctcgcccc ggcggccaac ggcggcaacc ggaccggccg acccttcacc
      121 ggggaccgtt ccggcgactg gctcttcgcg gcgatccacc gggcgggact ctccaaccag
      181 ccggaaagca ccggtcgcga cgacggactg gaactccgtg acaccttcgt caacgcggcg
      241 gtccgctgcg ccccgcccga aaacaagccc ctcccctccg agcgcgacaa ctgcgccccc
      301 tggctcgaag gcgagctgga actgctgacc gaagtgaagg tcgtgatcgc gctgggaggt
      361 ttcgcctggg aagccatgct ccggaccgtg cgcggcctcg gcggcgggat tccgaaaccg
      421 aagccgaagt tcggacacga aaccgaagtg accctcgagg ccccggcggg ggccgactgg
      481 tccgggccgg agcgctgggt cctgctcggc tgcttccacc cgagccccct gaacacgcag
      541 accggacggc tcagcgcggc gatgctcgac gacgtcttcg cgcgggcccg cgatctggcc
      601 gctgccggcc cgtaggctgg ccggcccggg gtcggccgcc gacgcccgag cgaccgcccg
      661 ggcccccgca acttgacgcg agccggtggc ctgcaagaat gtggccatgt tcgggagagg
      721 tcagggagtc cccggaagga gatggggccg cgtcgccttg ggtggtgtcg tggcagccgc
      781 cctctgcctc tttgcggtgc gccccgtgat cggcgacatg ctgaacagcg agccggaccc
      841 ggctccggtc gtggtcgcgg cgacacatcc ggcgacttct gcccaggtcg ccgacgcaag
      901 ggggctgccc gggacgggcc tcgctgccgg tgcctccgct tcgacgacca ggaagtcgaa
      961 gtccggaagg aacacaaggg tgatccgcac cgtctatcgc cgggtcaaga tcaactcccg
     1021 gatcaccgac caggaaatcc tgaccgagga aggcaagggc accttcgtcg ggatcaaatg
     1081 cccgaaagga tcgaaggcga tctccggcgg ggtgctgagc aagtacatca acctgctggt
     1141 ctcctcttcg gctccgaatc acccgttgac ccgcaagtac acaccgaaca tctggtggct
     1201 gaccgtggtc aacgtgaacg tcgacggaaa cggcggcacc ctcccctggc tcggggtcgt
     1261 caactgcctt tcaccggtga agctcggctc cggctgagcg ggaccgacta ctgcgcggcc
     1321 agggccgaag ccttgcccgc gcagtcgtag atcacattcg agtcggtcgt gctgtccgta
     1381 ccgttcgacg aagcagaggc ggtcccggac tgctgaccgg ccgaggctcc cgtggtagtc
     1441 gaatcggtgc cgctcgattc gagctggacc ggctcgcagg cctgggtcac cgcggtcatc
     1501 agctcggatg agccgacccg gccatcggcc ggcgaggtgc cctggtcgct ggcgagcacc
     1561 cagcggacct cgccgtcctt gaccctttcg gccagccagc cg
//
LOCUS       DAOTIP010000552         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_91457_length_1602_cov_3.345831, whole genome shotgun sequence.
ACCESSION   DAOTIP010000552 DAOTIP010000000
VERSION     DAOTIP010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91457_length_1602_cov_3.345831"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="P5138_09715"
     CDS             complement(<1..137)
                     /locus_tag="P5138_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HRV60895.1"
                     /translation="MAEASYPEDLKYHPEHDWARIDGGEATLGITWYAQDSLGEVVFF
                     EG"
     gene            complement(139..1224)
                     /gene="gcvT"
                     /locus_tag="P5138_09720"
     CDS             complement(139..1224)
                     /gene="gcvT"
                     /locus_tag="P5138_09720"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRV60896.1"
                     /translation="MTVESVLRRTPLHQTHVEAGAKMVPFAGWDMPVEYDGIREEHIA
                     VRTHAGIFDVSHMGQIEVEGPGSLTFLQRAVTNDVSKIEVGGAQYSLLLNDEAGAIDD
                     LFVYRLGHDRYLVITNAGNHMADLARLGQIARDFDVYVRDASSNYAMLAVQGPNARRI
                     LENTLHIDLPARMKVGARKVGRRPAIICGTGYTGEDGVELLIDPEIAPAIWTELVDAG
                     VVPCGLGARDTLRLEVCFHLHGNDLTEETDPISAGLGWACVESTGFIGADKVAVFRAN
                     GTDEKLAPFLIEGAGIPRPGNPVMIGDEAVGEVTSGTYSPSLEKGIGMAYLRADLAEP
                     GTELEIDVRGKRRAARVSSKPLYKKEK"
     gene            complement(1402..>1602)
                     /locus_tag="P5138_09725"
     CDS             complement(1402..>1602)
                     /locus_tag="P5138_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRV60897.1"
                     /translation="VDQSLVDEVRVTVIATGFEGFELLARTPQRVRRRYESRKRVSSE
                     DTDISNLRVGDSDIEVPPFLRD"
BASE COUNT          261 a          505 c          533 g          303 t
ORIGIN      
        1 ccttcgaaga agacgacctc gccgagcgag tcctgggcgt accaggtgat gccgagggtg
       61 gcctcgccgc cgtcgatccg ggcccagtcg tgctcgggat ggtatttcag gtcttcgggg
      121 tagctcgcct ctgccaagtc atttctcctt cttgtaaaga ggcttggagc ttacgcgtgc
      181 cgcacgacgc ttcccgcgca catcgatctc gagttcggtg ccgggctcgg cgagatcggc
      241 gcgcaggtag gccatcccga tgcccttctc cagggacggt gaataggtgc cactggtgac
      301 ctcgccgacc gcctcgtcgc cgatcatgac cgggttgccg ggcctcggaa taccggcccc
      361 ctcgatcagg aaaggggcga gtttctcgtc tgttccgttg gccctgaaaa cggccacctt
      421 gtcggccccg atgaaaccgg tcgactcgac gcaggcccag cccaagccgg ccgagatcgg
      481 atcggtctct tcggtcaggt cgttgccgtg gaggtggaaa cagacctcca gccgcagggt
      541 gtcccgggcg ccgaggccac agggcacgac cccggcgtcg accagttcag tccagatcgc
      601 cggagcgatc tcggggtcga tcaggagctc gaccccgtcc tcaccggtgt agccggtgcc
      661 gcagatgatc gccggtcgcc ttcccacttt ccgggccccc accttcatcc gggcgggcag
      721 atctatgtgg agggtgttct cgagtatgcg cctggcgttg gggccctgga ctgcgagcat
      781 cgcgtagttg gagctcgcgt ccctgacgta gacgtcgaag tcacgggcaa tctgaccgag
      841 tctggcgaga tccgccatgt ggttgccggc gttggtgatg accaggtagc ggtcgtgtcc
      901 gagccggtag acgaagagat cgtcgattgc tccggcttcg tcgttcagaa gaagcgaata
      961 ctgggctccg ccgacttcga ttttcgaaac gtcattggtg accgcccgct gcaggaaggt
     1021 cagtgagccc gggccctcga cctcgatctg gcccatgtgg gatacgtcga agatcccggc
     1081 gtgagtccgg accgcgatgt gttcttccct gattccgtcg tattcgaccg gcatgtccca
     1141 gccggcgaag ggcaccatct tcgccccggc ttccacatgt gtctggtgga gcggagtgcg
     1201 gcgaaggacg gattcaacgg tcaccctccc gatattaggg aagaactccc cgattgcgga
     1261 tggttcgtct cggaggccct aggcgcggct ggcgaggaga caggccggaa atggccgcga
     1321 cagtacgcgg gtacacgagc tgccatttcc ggccgccgac gaagccaggc gtgcataggg
     1381 gcccgagacg ggccttccac ctcagtcccg gaggaaggga ggcacttcga tgtcgctgtc
     1441 tccgacccgg aggttgctga tgtccgtgtc ctcggaggag acccgcttgc gggactcgta
     1501 gcggcgccgg accctctgcg gggtgcgggc gagcagttcg aagccttcga agccggttgc
     1561 gatcacggtc acccggacct cgtccaccag tgactggtcg ac
//
LOCUS       DAOTIP010000553         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_91768_length_1598_cov_3.587168, whole genome shotgun sequence.
ACCESSION   DAOTIP010000553 DAOTIP010000000
VERSION     DAOTIP010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91768_length_1598_cov_3.587168"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /gene="eno"
                     /locus_tag="P5138_09730"
     CDS             <1..324
                     /gene="eno"
                     /locus_tag="P5138_09730"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225678.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRV60898.1"
                     /translation="RLKRGIDLGVANSILVKVNQIGTLTETLEAIRMASDAGYTSVIS
                     HRSGETEDTTIADLAVATGAGQIKTGAPSRSDRVAKYNRLLRIEEELGTGARFGGTDV
                     FKRGS"
     gene            358..735
                     /locus_tag="P5138_09735"
     CDS             358..735
                     /locus_tag="P5138_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRV60899.1"
                     /translation="MSVRAQTYGEARRPPRRRPAPRKNAGRPGAGRIQWDRFGRILLA
                     IVIVLLFVAMVKPAWNAVHTYRQTSQAKIRLHQAQAEHAMLERQVKRARSEATLQREA
                     RRQGMIRPGEEAWVVNGAKGESR"
     gene            761..>1598
                     /locus_tag="P5138_09740"
     CDS             761..>1598
                     /locus_tag="P5138_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60900.1"
                     /translation="MSFPDFISGLVNLALVGLFAGFTAFRLRARLMPGWSGSPARLVE
                     AVLGVGLLVISSEMLGLFGLLEPLPLVILLGLLALVAWLTVIPRPAGPGDPVPPAPAV
                     STAAQILAFVAAFVVFAQWGAFTSYNLDHGITNFDSVWYHMPFAADMARTGSVTGFFH
                     TETVFVNWFYPQNSELIHAVGIVLTGRDFVSIFINMGWLALGLLAGWCVGRPYDRPHL
                     TMLGVAVLMAAHTLVVREPGTAKNDVMAAALVLASAAIMLNRSAARPDPPGRVSPDWA
                     MAV"
BASE COUNT          240 a          550 c          520 g          288 t
ORIGIN      
        1 cgcctgaaac ggggcatcga cctcggcgtc gccaattcga tcctggtcaa ggtgaaccag
       61 atcggcaccc tgaccgagac cctggaggcg atccgcatgg cctccgatgc cggctacaca
      121 tcggtgattt cacatcgatc gggtgagacc gaggacacga cgatcgccga cctcgcggtc
      181 gcgaccgggg ccgggcagat caagaccggc gcgccttccc gctcggatcg ggtcgccaag
      241 tacaaccggc tgctcaggat cgaagaggaa ctgggcaccg gcgcccgttt cggcggcacg
      301 gacgttttca agcggggtag ttgaaggaga tgaccgtccg gcctagaagg gtcggacgtg
      361 tcagtgcgcg cccagactta cggcgaggcg cggcgtccgc cacggcgccg gcctgccccc
      421 cggaagaatg ccggccggcc cggtgccgga cgaatccagt gggaccgctt cggtcggatc
      481 ctgctggcga tagtcatcgt gctcctcttt gtggccatgg tcaaaccggc ctggaacgca
      541 gttcacacct accggcagac gagtcaggcc aaaatccggc tacatcaggc ccaggccgag
      601 cacgcaatgc tggaacgtca ggtcaagcgg gcccgcagcg aagcgacgct tcagcgcgag
      661 gccaggcgcc agggcatgat ccggccggga gaagaggcct gggtcgtaaa cggcgccaag
      721 ggcgagtccc ggtaggggac tgtggggccg gtggcctcgg ttgagcttcc ctgacttcat
      781 ttccggcctg gtcaacctgg cactggtggg gctctttgcc ggcttcaccg cctttcgcct
      841 gagggctcgc ctcatgcccg gctggagtgg ctcgccggcc cggctggtcg aggcggtact
      901 cggggtcggt ctgctggtca tctcctccga gatgctcggc ctgttcggcc tgctcgaacc
      961 acttcccctg gtcatcctgc tgggcctcct ggccctcgtt gcgtggctga cagtgatccc
     1021 ccgcccggcc gggccgggtg acccggtgcc gcccgcaccg gccgtctcga ccgcagccca
     1081 gatactggcc ttcgtcgccg ccttcgtcgt cttcgcccag tggggtgcgt tcacctccta
     1141 caacctcgat cacggcatca ccaatttcga ctcggtctgg taccacatgc ccttcgcagc
     1201 ggacatggcc cggaccggct cggtgaccgg tttcttccac accgagacgg tcttcgtgaa
     1261 ctggttttat ccacagaatt cggagttgat ccacgcagtc ggaatcgttc tgaccggccg
     1321 cgatttcgtt tcgatcttca tcaacatggg ctggctcgcc ctcggcctgc tcgccggctg
     1381 gtgcgttggc cgtccctacg accgtccgca cctgaccatg ctcggtgtcg ccgtgctgat
     1441 ggccgcccat accctggtgg tccgcgagcc cggtaccgcc aagaacgacg ttatggcggc
     1501 cgccctcgtc ctcgcctcgg ccgcgatcat gctgaaccgc tccgccgccc ggccggaccc
     1561 gccggggagg gtgtcgccgg actgggcgat ggcggtcg
//
LOCUS       DAOTIP010000554         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_91783_length_1598_cov_2.864550, whole genome shotgun sequence.
ACCESSION   DAOTIP010000554 DAOTIP010000000
VERSION     DAOTIP010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91783_length_1598_cov_2.864550"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="P5138_09745"
     CDS             complement(<1..226)
                     /locus_tag="P5138_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HRV60901.1"
                     /translation="MSIRILVLGGGGREHAIVKALARSPQSPEIFCAPGNPGIAADAT
                     CLPDLDPVDPSAVTAKAAELEEARKDPTKSM"
     gene            complement(223..504)
                     /locus_tag="P5138_09750"
     CDS             complement(223..504)
                     /locus_tag="P5138_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HRV60902.1"
                     /translation="MPTFVMLTNLTSEGVKTLKSHPGRVAEVNREVEALGARVVAQYA
                     TLGQYDFVTIIEAESEEVMAKVSVEMGSRGTMSSQTLAALPAERLAEVL"
     gene            complement(611..1162)
                     /locus_tag="P5138_09755"
     CDS             complement(611..1162)
                     /locus_tag="P5138_09755"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRV60903.1"
                     /translation="MALKHAVLAALSAEPGSGYELSKRFDASVANFWTASAQQVYREL
                     DRLESDGLVRARTVRQQKRPDKRVFRITGEGDRELTDFIRAPNRPTVIRDDLLVKVAS
                     LSEANAADVSLAVRERVETSRQKLAMYENLREVLLGGVTEQRFLADGPGRPGFGPFLA
                     LKRGIAFERGNVRWAREVLGLLG"
     gene            1244..>1598
                     /locus_tag="P5138_09760"
     CDS             1244..>1598
                     /locus_tag="P5138_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60904.1"
                     /translation="MSEEIWQRVVPPVPEDPDVVVIGSGLAGLTAAAYLAANDRKVLV
                     LEQYDVAGGCSQTFRRKKEWQFDVGLHYIGGVKTGEIGRILGGLGLDERIEFPELDPD
                     GFDTFVFPDLEFKMPK"
BASE COUNT          266 a          509 c          539 g          284 t
ORIGIN      
        1 ccatcgactt cgtggggtcc ttgcgggcct cctcgagctc cgccgccttc gcggtcaccg
       61 ctgaagggtc gaccgggtcg aggtccggca ggcaggttgc gtcagccgcg attcccgggt
      121 tgcccggcgc gcagaagatc tccggcgact gaggggagcg ggcgagcgcc ttgacgatgg
      181 cgtgctcgcg gccgccgccg ccgaggacga ggatgcggat gctcaaagaa cttcagcgag
      241 ccgctcggcc gggagcgcag cgagggtctg gcttgacatg gtgccgcggg aacccatctc
      301 gacggagacc ttggccatca cttcctcgga ctcggcctca atgatcgtca cgaagtcgta
      361 ctggccgaga gtcgcgtact gggcgacgac cctggcgccg agtgcctcga cttcgcgatt
      421 gacctcggcc acgcggcccg gatggctctt caacgtcttg acaccctcgg aagtcaggtt
      481 ggtcagcatc acgaaagtcg gcattggtgt cctctccttg tttgcgactg gttcagggac
      541 tgttacccgc tctgccgcca gcatatgcga gcgcagcccc gggccgtagc ggccggtcgc
      601 gaaggtgccg ctacccgagc aggccgagga cctcgcgggc ccagcggacg ttgcctcttt
      661 cgaaggcgat tccgcgttta agggcgagga aggggccgaa accgggccgg ccggggccat
      721 ccgcgaggaa tctctgctcg gtaactccgc ccagcagcac ctcccgcagg ttctcgtaca
      781 tcgcgagctt ctgccggctg gtctcgaccc gctcgcgaac cgcaagcgat acgtcggcgg
      841 cattggcctc gctcaggctc gcgaccttga ccagaaggtc atcgcggatc acggtcggcc
      901 ggttgggcgc gcgaatgaaa tcggtgagtt cgcggtcgcc ctcgccggtg atgcggaaga
      961 cccgcttgtc cgggcgtttc tgctggcgga cggtccgggc ccggaccagg ccatcggatt
     1021 ccaggcggtc cagttcgcgg tagacctgct gggcggaggc cgtccagaag ttggcgaccg
     1081 aggcgtcgaa gcgcttcgaa agttcgtaac ccgaacccgg ttccgcgctc agcgctgcga
     1141 gcaccgcatg cttcagcgcc atcgggctgg acttccgttg ctgatcgtcc tatactcaac
     1201 aaattgagta atcacatggt tgattatagg agaggtgaag acgttgagtg aggaaatctg
     1261 gcagcgggtg gtgcccccgg tccccgaaga tccggatgtg gtggtgatcg gctccggcct
     1321 tgccggactg acggcggccg cctacctcgc cgccaacgac cgcaaggtgc tggtactcga
     1381 gcagtacgac gtcgccggcg gctgcagcca gacctttcgc cgcaagaagg agtggcagtt
     1441 cgacgtcggt cttcactaca tcggcggggt aaagacgggg gagatcggcc gcatcctcgg
     1501 gggcctggga ctcgacgagc ggatcgagtt tcccgagctc gaccccgacg gattcgacac
     1561 cttcgtcttc cccgacctcg agttcaaaat gccaaagg
//
LOCUS       DAOTIP010000555         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_91870_length_1597_cov_2.731518, whole genome shotgun sequence.
ACCESSION   DAOTIP010000555 DAOTIP010000000
VERSION     DAOTIP010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91870_length_1597_cov_2.731518"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="P5138_09765"
     CDS             <1..1459
                     /locus_tag="P5138_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRV60905.1"
                     /translation="PVISHDTLVAAYLLEPSRRNYHLDELAVQAGLAPSGVDGDDGQM
                     SLIADEQETGSIAPEIAATVWNLAKDQRPKLEEAGLLDLLEQVEQPLIRVLAAMERIG
                     LKLDTDRVAEMASGLEDRILELEREIHELAGHEFTIGSPQQVGQVLFEELGLTRKRRG
                     KTGFSTDARVLAQIREEHAIVALIEEWRELTKLKNTYLEPLPAAIDPATGRVHTTFMQ
                     TAAPTGRISSIQPNLQSIPIRSEVGRPLRGCFVAEPGNLLTSLDYSQVELRVLAHVAD
                     EEVLKGFFRAGEDVHSATAAQVFEIDPGEVTESQRSKAKMVNFGIVYGLTGFGLADRL
                     NIPRAEGEEFVKRYHERFPAVTAFRERVVEEATETGFVKTLLGRRRPLPELRSNQAQV
                     RAQGVRLAMNTVVQGTAADIIKIAMIRSHEALLDEDLSSRLVLQIHDELLFEGPAGET
                     EAVTELARREMVGAFDLDPPLEVDAGSGESWLAAK"
BASE COUNT          284 a          530 c          536 g          247 t
ORIGIN      
        1 cccggtgatc agccacgaca ccctggttgc cgcatacctg ctcgaaccct cgaggcggaa
       61 ctaccacctg gatgaactgg cggtccaggc aggattggcg ccgtcggggg tggacggcga
      121 cgacggccag atgtcgctga tcgccgacga acaggaaacc ggttcgatcg ctcccgagat
      181 cgctgccacg gtctggaacc tcgcaaagga ccagcggccg aagctggagg aggcggggct
      241 tctcgacctg ctcgaacagg tggagcagcc gttgatccgg gtgctcgccg caatggagcg
      301 gatcggtctc aagctcgaca ccgaccgggt cgcggagatg gcatccggac tggaggaccg
      361 gatcctcgag ttggaacgcg aaatccacga actggccggg catgagttca cgatcggctc
      421 gccgcaacag gtcggccagg tgcttttcga ggagctgggc ctgacccgca agcgacgggg
      481 caagaccgga ttctcgaccg acgcgcgggt gcttgcccag atccgcgaag aacacgcgat
      541 cgtcgccctg atcgaggagt ggcgcgagct gaccaagctg aagaacacct acctggagcc
      601 gctaccggcc gcgatcgatc cggcgactgg ccgggtccac acgaccttca tgcagaccgc
      661 cgccccgacc gggcggatct cctcgatcca gccgaacctt cagagcattc cgatccgctc
      721 cgaggttggc cggccccttc ggggctgctt cgtggccgag cccggcaacc tgctcacctc
      781 gctggactac agccaggtgg aacttcgggt gctcgcccac gtcgccgacg aagaagtcct
      841 caagggtttc ttccgggccg gggaagacgt gcattccgcg accgccgccc aggtcttcga
      901 gatcgacccg ggagaggtca ccgagtcgca gcgctccaag gcgaagatgg tcaacttcgg
      961 gatcgtctac ggcctgaccg ggttcgggct ggccgaccgg ctcaacatcc cccgggccga
     1021 gggcgaggaa ttcgtcaagc gctaccacga gcgttttccg gcggtgaccg cctttcgcga
     1081 gcgggtggtc gaggaagcga ccgagaccgg cttcgtcaag accctgctcg gccgacggcg
     1141 cccgctgccg gaactccgct cgaaccaggc ccaggtcagg gcccaggggg tgcggttggc
     1201 catgaacacg gtggtgcagg gcaccgctgc cgacatcatc aagatcgcga tgatccgctc
     1261 tcacgaggcg ctgctcgacg aggacctctc gagccggctg gtccttcaga tccacgacga
     1321 gctgctgttc gagggcccgg ccggggagac cgaagcggtg accgaactgg cccggcggga
     1381 aatggtcgga gccttcgatc tcgatccgcc cctggaagtc gacgccggct cgggcgagag
     1441 ctggctggcc gcgaagtaac cacgcaaggg gactgagccg tgagttccgc cctggttgcc
     1501 ctgctgatcg tcctggtggg cgtcctcttc gccgcacagg caccaatcaa cagcggtctc
     1561 ggtcgttcga ccggaccgct cgctgcggcc ttcgtca
//
LOCUS       DAOTIP010000556         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_92192_length_1593_cov_3.745774, whole genome shotgun sequence.
ACCESSION   DAOTIP010000556 DAOTIP010000000
VERSION     DAOTIP010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92192_length_1593_cov_3.745774"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="P5138_09770"
     CDS             <1..1137
                     /locus_tag="P5138_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRV60906.1"
                     /translation="RVLDPGVGTGELLRATLDREPGIETIGWDVDETAIAAAGELVPE
                     AELEVRSALEAGEPGDGGFDLVIANPPYFQLRLDPSEKSRFARVISGRANIFALFFQV
                     GIELLKPGGRLAFIVPPSMNSGAYFEGLREFLTESAEVVDLTLLEGTDIFEGANTAVQ
                     LLVLRKHGNEVGRIFGTGTENPTHRKFVFRREVPGAGFRRVLFSPDPARLAAEFEGRM
                     TLWELGLEAFTGPVIWNENRDRLADGPGPGRVPLIWSRSIGAEGLLTGLSRSAGKAGY
                     YDTKAGGREPLTGPAIVVNRVVGAVGRGELRAALVPEGLEFLAENHVNVIRRREPGEN
                     GGDFPGWEAVLDSLRRPGVAERVRLLTGNTQISARELTHLLPLE"
     gene            complement(1166..>1593)
                     /locus_tag="P5138_09775"
     CDS             complement(1166..>1593)
                     /locus_tag="P5138_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV60907.1"
                     /translation="TRLVAAFRDFLGGVSPTIAEIEATASILLRDRATEFLLIGDPES
                     GFVQTRFRLSVWTGAEDAWLEDLFVEESARGKGYGRALVEAAVERARSRGCDRIQLDV
                     NQANVNALKLYESCGFKPVHNPAKWGEAPDFFYTREIDR"
BASE COUNT          260 a          508 c          562 g          263 t
ORIGIN      
        1 cgggtgctcg atccgggcgt cgggaccggc gaactgcttc gtgccacgtt ggatcgggag
       61 cccggaatcg agacgattgg ctgggacgtc gacgaaactg cgatcgcagc ggcaggggaa
      121 ctcgttccgg aagccgaact ggaagtccgg tccgcccttg aagccggcga gccgggcgac
      181 ggcgggttcg acctggtgat cgccaacccg ccctacttcc agcttcgcct ggatccgtcg
      241 gagaagagtc gcttcgcaag ggtgatctcc ggacgggcca acatctttgc cctcttcttc
      301 caggtcggca tcgaattgct gaagcccggg ggcaggctcg ctttcatcgt gccgccgtcg
      361 atgaacagcg gcgcctattt cgagggtctc cgtgagttcc tgaccgaaag cgccgaagta
      421 gtcgacctca cccttctgga gggaaccgac attttcgagg gggccaatac ggcagtccag
      481 ctcctggtcc tgagaaagca cggaaacgag gtgggtcgga ttttcggcac cggtaccgaa
      541 aatccgaccc accggaaatt tgtcttccgg cgggaggtgc ccggggcggg cttccgccgc
      601 gtgctcttca gcccggaccc ggcccggctg gcggccgagt tcgaaggccg gatgaccctc
      661 tgggagctcg gtctcgaggc cttcaccggc ccggtgatct ggaacgagaa ccgggaccgt
      721 cttgccgacg ggcctggtcc gggccgggtg cccctgatct ggtcacgcag catcggtgcg
      781 gaggggttgc tgacgggcct gagccgatcc gcgggcaagg cgggctacta cgacacaaag
      841 gccggcgggc gcgagccgct gacgggaccg gcgatcgtgg tcaaccgggt cgtcggcgcg
      901 gtcggccggg gggagctgcg ggcggcgctg gtccccgagg gactcgagtt cctcgccgaa
      961 aaccacgtca acgtgatccg caggcgggaa cccggggaaa atggcgggga tttccccggc
     1021 tgggaggcgg ttctggactc gcttcgtcgt cccggcgtgg ccgaaagggt gaggcttctg
     1081 accggcaaca cccagatctc ggcccgggag ctgacccacc tgctgccgct cgagtgacgg
     1141 gcccgatccc cgtgactcct ccggatcagc gatcgatctc gcgggtgtag aagaagtcgg
     1201 gcgcttcgcc ccacttggcc gggttgtgga ccggcttgaa cccgcaggat tcatagagct
     1261 tcagggcgtt gacgttcgcc tggttgacgt cgagctggat ccggtcgcat cctctggacc
     1321 gggcccgctc caccgccgct tcgaccaggg cgcgtccgta ccccttgccg cgggcggatt
     1381 cctcgacgaa gagatcttcg agccaggcgt cctcggcccc ggtccacacc gaaagccgga
     1441 accgggtctg gacgaagccg gactcgggat cgccgatcag gaggaactcc gtcgcgcgat
     1501 cacgcagcag gatcgaggcg gtcgcttcga tctcggcgat cgtcggcgag acgccaccga
     1561 ggaagtcgcg gaaggctgcg accagccggg tga
//
LOCUS       DAOTIP010000557         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_92221_length_1593_cov_2.604681, whole genome shotgun sequence.
ACCESSION   DAOTIP010000557 DAOTIP010000000
VERSION     DAOTIP010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92221_length_1593_cov_2.604681"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="P5138_09780"
     CDS             complement(<1..905)
                     /locus_tag="P5138_09780"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRV60908.1"
                     /translation="MPIEQGYLALDEESEVRLRRAGDALKLTVKHGHGESRQETEVDV
                     DQATFDELWPESEGRRVEKERHRIELEGGLVAELDIYSGDLDGTSVVEVEFDSQSAAR
                     DFRPPAWFGRELTGDRLWANQGLAIHGRPPKGLEFRLRPDEEPATGVGRVITARAAQA
                     VAAVRQAGAAEDSAPYVHEARKSLKKVRSALRLLRGIIPDEERSAFNSDCRKAGNSLS
                     GARDAEVKMATLGLVLGKDGEADLADDWMAELEEEAEVHRGGLTAANLKELEDSIEGV
                     RRGFLGRETGAAGEAVAGNVGRGYRR"
     gene            complement(1037..>1593)
                     /locus_tag="P5138_09785"
     CDS             complement(1037..>1593)
                     /locus_tag="P5138_09785"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936107.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRV60909.1"
                     /translation="KGDTVVDEDEAIRPGTTAESLSGLRAVGGDDATHTAGNAPGVND
                     GAGALVLASDEWAEANGKQPMATIVGYGQIGDEYACLAKTPALAAKAALDRLGKSPGE
                     VDLWEVNEAFASVTLNTIKMLDLDPEKMNVHGGAVALGHPIGASGARVIGVLVRELQR
                     RGGGLGCAAICSGGGQGDAILVEV"
BASE COUNT          242 a          582 c          496 g          273 t
ORIGIN      
        1 cggcgataac cacgacccac gttgccggcg accgcttccc cggcggcgcc cgtttcccgg
       61 ccgagaaagc cgcgccggac tccctcgatc gagtcctcca gttccttaag gttcgcggcc
      121 gtgaggccac cccggtggac ttccgcttcc tcttcgagct ccgccatcca gtcatcggca
      181 aggtccgctt cgccgtcctt tcccaggacc aggccaagcg tcgccatctt gacttcggcg
      241 tccctggccc cggaaaggct gtttcccgcc ttccggcagt ccgagttgaa ggcggaccgc
      301 tcttcgtcgg gaatgatccc cctgagcagg cgcagcgccg atcgcacctt cttcagactc
      361 ttgcgggcct cgtgaacgta cggtgctgaa tcttcagccg cgcccgcctg cctcaccgct
      421 gccaccgcct gggcagcccg ggcggtgatc acccggccga ccccggtcgc cggttcctca
      481 tccggtcgaa gccggaattc gaggcccttg ggcggcctgc cgtgaatcgc gaggccctgg
      541 ttggcccaga gacggtcacc ggtcaactcc cggccgaacc aggccggcgg ccggaagtcg
      601 cgggctgcgg actgcgaatc gaactcgacc tcgaccaccg aggttccgtc gaggtctccc
      661 gagtagatgt cgagctcggc taccagcccg ccctcaagct cgatcctgtg acgctccttc
      721 tcgacccggc ggccttccga ctccggccag agctcgtcga aggtggcctg gtcgacatcg
      781 acttcggttt cctgccgcga ctcgccgtga ccatgcttga cggtcagctt cagggcgtca
      841 ccagcccgcc gcagccggac ttctgattcc tcgtcgagag ccaggtatcc ctgctcgatc
      901 ggcatccgga aagactccga aagatcctcc ggaacccgat caatcagaaa cttgcgctcg
      961 atctccaagg cgccccgaga ttacccggct gcgcactgat tacccaaggg gcaaccaggt
     1021 tatgtgccct gtgagctcag acctcgacga gaatggcgtc gccctgaccg ccgcccgagc
     1081 agatcgcggc acaaccgagg ccgccgccgc gacgctggag ctcgcggacc aggacgccaa
     1141 tgacccgggc gccggaagcg ccgatcggat ggccgagggc gaccgcgccg ccgtgcacgt
     1201 tcatcttttc cgggtcgaga tcgagcatct tgatcgtgtt cagggtgacc gaagcgaagg
     1261 cctcgttgac ctcccagaga tcgacctcgc cggggctctt gccgagccgg tcgagagcgg
     1321 ccttggccgc cagggccggg gtcttggcga ggcaggcgta ctcgtcaccg atctggccgt
     1381 agccgacgat cgtcgccatc ggctgcttgc cgttggcctc ggcccactcg tcggaagcca
     1441 gcaccagggc gccggcaccg tcgttgactc ccggggcgtt gccggcggtg tgggtagcgt
     1501 cgtcaccacc gaccgcgcga aggccggaaa ggctctcggc ggtggtgccg gggcgaatcg
     1561 cctcgtcctc gtcgaccacc gtgtcgccct tgc
//
LOCUS       DAOTIP010000558         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_92819_length_1586_cov_2.417374, whole genome shotgun sequence.
ACCESSION   DAOTIP010000558 DAOTIP010000000
VERSION     DAOTIP010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92819_length_1586_cov_2.417374"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..958)
                     /locus_tag="P5138_09790"
     CDS             complement(59..958)
                     /locus_tag="P5138_09790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV60910.1"
                     /translation="MEQPELEIVVVSHGAADLLRSCLLSLRRFPPAPPTRVTVVDSGS
                     TDGTPDMVEAEFPEVRLIRLGNVGFSAANNAVLMDSGRGNGPRMFLLLNPDTEVREGT
                     IDAAISRLDRSPEVGMVGVRLVTADGTFDHASKRAFPTPLAALAHFTGIGRLPGAPDS
                     LTQYRATWLGENEAGPVEAVNGAFMLVRREAADQVGPLDEGYWLYMEDLDWCRRFGEA
                     GWTVFYEPAGEALHVKGGSSPARRRPRQEIAFHRGMGRFYRRFDAPGNPSLVNFAVYL
                     GIAAKLAVSLLITALSPGPGKRV"
     gene            complement(975..>1586)
                     /locus_tag="P5138_09795"
     CDS             complement(975..>1586)
                     /locus_tag="P5138_09795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRV60911.1"
                     /translation="DPPRSTMAIKRTMDLLVSISMLTIMAPFLGLAALAIRLDSRGPV
                     FFRQVRVGRDGRNFTMYKLRTMTADAEEQLDSLIDLDALDEPAFKIADDPRVTRVGRL
                     LRRSSFDEVPQFINVLKGDMALVGPRPEEEAVVALYDERQRQRLRVKPGLTGPMQVAG
                     RGGLTFEERLALERDYVDNLTITGDIKILLRTPQAVIRGDGAF"
BASE COUNT          257 a          537 c          537 g          255 t
ORIGIN      
        1 cgcctcaagt cggcccttgg ggaaggcaag tcgaccggat cctgaaccgg gttcccgttc
       61 agacccgttt tccggggccg ggagagagcg ccgttatcag caggctgacc gcgagctttg
      121 cggcgatccc gagatagacc gcgaaattga ccaggctggg gtttccgggg gcgtcgaagc
      181 gccggtagaa gcgccccatg ccgcggtgaa aggcgatctc ctgccgcggc cggcgacggg
      241 ccgggcttga gccgcccttg acatgcaggg cctccccggc cggctcgtag aacaccgtcc
      301 agcccgcttc gccgaagcgt cggcaccagt ccaggtcctc catgtaaagc cagtagccct
      361 cgtcaagcgg cccgacctga tcggctgcct cccgccggac cagcatgaag gcgccgttca
      421 ctgcctcgac cggcccggcc tcgttctcgc ccagccaggt cgcccggtac tgggtgagac
      481 tgtcgggcgc tcccgggagc cgcccgatcc cggtgaaatg cgcaagtgcc gccagcgggg
      541 tcgggaacgc ccgcttgctg gcgtggtcga aagtcccgtc ggcggttacg agccggactc
      601 cgaccattcc cacctccggc gaccggtcga gacgggagat cgccgcgtcg atcgtgcctt
      661 cacgtacttc ggtatcggga ttcaacaaca ggaacatccg ggggccgttc ccccggccgg
      721 aatccatgag aaccgcgttg ttggcggctg agaaaccgac gttgccgagc cggatcagcc
      781 ggacctcggg gaattccgct tcgaccatgt cgggggtgcc gtcggtcgat cccgaatcga
      841 ccacggtcac ccgggtgggt ggagcgggcg ggaaccggcg cagcgaaagc aggcagctcc
      901 ggagcaggtc ggcggcaccg tgactgacca cgacgatctc cagctcgggc tgctccatgg
      961 cggtcaggct attgctagaa ggccccgtcg ccccggatta cggcctgggg agtgcggagc
     1021 aggatcttga tgtcgccggt gatcgtcagg ttgtccacat agtcccgctc cagggcgaga
     1081 cgctcttcga aggtcaggcc gccccgcccg gccacctgca tcggcccggt cagcccgggc
     1141 ttcacccgga gtcgctggcg ctgcctctcg tcatagaggg caacgaccgc ctcctcctcc
     1201 ggccgtggac cgaccagggc catgtcgccc ttcagcacgt tgatgaactg gggcacctcg
     1261 tcgaagctcg accgtcgcag caaccgcccg acccgggtca ccctgggatc gtctgcgatc
     1321 ttgaaggccg gttcgtcgag ggcgtccagg tcgatcagcg agtcgagctg ctcttcggcg
     1381 tcggcggtca tggtgcggag cttgtacatc gtgaagttgc ggccgtcccg tcccacccgc
     1441 acctgccgga agaagaccgg gccgcgtgag tcgagccgga tggccaaagc ggccaggccc
     1501 aggaacggcg ccatgatggt cagcatcgag atcgagacga gcaggtccat ggtgcgtttg
     1561 atcgccatcg tcgatcgcgg cgggtc
//
LOCUS       DAOTIP010000559         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_93108_length_1582_cov_4.517354, whole genome shotgun sequence.
ACCESSION   DAOTIP010000559 DAOTIP010000000
VERSION     DAOTIP010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93108_length_1582_cov_4.517354"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="P5138_09800"
     CDS             <1..822
                     /locus_tag="P5138_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV60912.1"
                     /translation="PDGPETHWMVWSDNTEYGESVMTLWDTDWNEIMNERRAAFTMAA
                     SHELRGPLTTLQGFAEILNMDTGNMRPEQAEAAAIIESTARHLAILVEDVFDLSRNSF
                     GELRLNLCDIDLARVVDEVCSITRPGVEGRGQSLDAEIDGELPRLQADEARANQMITN
                     LINNASVHNAEGVSIKIRAFVEDGYVAVEVEDDGKGLPFDDPEEAFRTFRRGEESTAG
                     DRTGSGIGLSITKRLIQLHRGRIEVESTRGAGTSFTLWFPIDRDSALDPGEPGPA"
     gene            819..1169
                     /locus_tag="P5138_09805"
     CDS             819..1169
                     /locus_tag="P5138_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV60913.1"
                     /translation="MSRILVIEDSPSIALLLRRRIEMEGHEVEVRASGREALDLIMGG
                     PLPDLVLADVMMPGMDGMETLGQIKQSHPGLPVILVTGQQMTRAETGQADALVSKPID
                     FEALFREISGLVPG"
     gene            complement(1176..>1582)
                     /locus_tag="P5138_09810"
     CDS             complement(1176..>1582)
                     /locus_tag="P5138_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRV60914.1"
                     /translation="PPRRRRRGETPAPLDEQPAVAVEEVEEFDEVVEVPLNAPEESRE
                     DSVSENIPETPDGAVKLFVNRGSRSGITEEDLRWALREGAVLEDEQVADVRVLERFSF
                     VEVNGDVAERTVEFLDGTRLKGSGIRVEVARS"
BASE COUNT          292 a          539 c          493 g          258 t
ORIGIN      
        1 cccgacggac ccgaaaccca ctggatggtc tggtccgaca acacggagta cggcgagtcg
       61 gtgatgactc tctgggacac cgactggaac gagatcatga acgaacggcg ggccgccttc
      121 accatggctg cctcacacga gctccggggg ccgctgacca cgcttcaggg cttcgccgag
      181 attctcaaca tggacaccgg caacatgagg cccgagcagg ccgaggccgc cgcgataatc
      241 gagagcactg cccgacatct cgcgatcctg gtcgaagacg tcttcgacct gagtcgcaac
      301 agtttcggcg agctgcgcct caacctctgc gatatcgatc tcgcccgcgt ggtcgatgaa
      361 gtgtgctcga tcacccggcc aggcgttgag ggccggggcc agtcgctgga tgccgagatc
      421 gacggtgagt tgccccggct ccaggctgac gaggcgcggg ccaaccagat gatcaccaac
      481 ctgatcaaca acgcctcggt ccacaacgcg gagggcgtct cgatcaagat ccgggccttc
      541 gtcgaggacg gatacgtggc ggtcgaggtc gaggacgacg gcaaaggtct cccgttcgac
      601 gatcccgagg aggccttccg cacctttcgc cggggcgagg aatccaccgc gggcgaccgc
      661 acgggttcag gaatcgggct ctcgataacc aagcgcctga tccagctcca tcgcggaagg
      721 atcgaagtcg aatcgacccg cggagccgga acgagcttca ccctctggtt tccgatcgac
      781 cgcgactcgg ccctcgatcc cggcgaaccg gggccggctt gagccgaatc ctggtcatcg
      841 aagattcccc ttcgatcgcg cttttgctca ggcgccgcat cgaaatggaa ggccacgagg
      901 tcgaggtccg ggccagtggg cgggaagctc tcgacctgat catgggcgga ccgctgccgg
      961 atctggtgtt ggccgacgtg atgatgccgg ggatggacgg catggaaacc ctcgggcaaa
     1021 tcaagcagtc ccatccgggc ctgccggtga ttctggtcac cggtcagcag atgacccggg
     1081 ccgaaaccgg ccaggccgac gcactggtca gcaagccgat cgacttcgaa gcgctgttca
     1141 gggagatcag cggactcgtc cccggttagc cggtgtcagc tgcgggcgac ctcgaccctg
     1201 atcccggaac ccttgagccg cgtgccgtcg aggaactcga cggtcctttc ggccacgtca
     1261 ccattcactt ccacgaagga aaacctttcc agcaccctga cgtcggccac ctgctcgtcc
     1321 tccagtacgg ctccctcgcg cagggcccac cgaaggtcct cctcggtgat tccggaccgc
     1381 gaacccctgt tcacgaacag cttcacggcg ccgtccggag tctccgggat gttctctgaa
     1441 acggagtctt cacgcgactc ctccggcgcg ttcagcggca cctcgacgac ctcgtcgaac
     1501 tcctcgacct cctcaaccgc caccgcgggc tgctcgtcga gcggagcggg tgtctccccc
     1561 cggcgccggc gacgcggcgg ac
//
LOCUS       DAOTIP010000560         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_93382_length_1579_cov_3.450131, whole genome shotgun sequence.
ACCESSION   DAOTIP010000560 DAOTIP010000000
VERSION     DAOTIP010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93382_length_1579_cov_3.450131"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1092)
                     /locus_tag="P5138_09815"
     CDS             complement(115..1092)
                     /locus_tag="P5138_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60915.1"
                     /translation="MPRLVGGDLVFRLRGPWLERLLVIEDQPMIARVREGHRGEFVFA
                     AEPVDPALLEGPGSAGLREAGEEALEAGLDRMRFSLGVDDDLVGFLNAFREDPLLGRA
                     LNGRLQNRPMRRPVPWEALLWAITEQLIEYRRAARIQRQMIRRFGIKFSPGVTVSSSG
                     TDWRLPHSPRGRQEASLATVPSPQSISAAAPAELEACGLSGKRAVAMVKVAREVASGR
                     VDPSDPAGDRRLLAIPEIGPWTVACLALRGRGEPDALLAGDLSQVKLIGYLKGLGRLA
                     EVEEVEEFYAPYAPWRGLAGEYMSMAFGSAVHGPGNRARIRQQSLRRAA"
     gene            complement(1145..1456)
                     /locus_tag="P5138_09820"
     CDS             complement(1145..1456)
                     /locus_tag="P5138_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60916.1"
                     /translation="MAEEVDGELISEEDVTASQSGGEVRALPREYESGGALPALKNEV
                     TTAAIAVAGGAIAGAATVAAVRAVSGGSKRKARRLGRRARPANVVASRSFLVDVHVLG
                     R"
BASE COUNT          263 a          580 c          506 g          230 t
ORIGIN      
        1 ctggcaggcc ttcatcaact ggatggtcca ggagggagtg ttgaacagct cccagcgcgc
       61 cgtcgatgcg ctcaccaacg agctgcttcc gaccaggaag gtcgaagtag gcagctaggc
      121 ggcgcggcgg agggactgct ggcggatgcg ggcccggttg cccggtccgt ggacggccga
      181 gccgaaggcc atcgacatgt actcgccggc gagccctcgc cagggcgcgt acggggcgta
      241 gaactcctcg acctcctcga cctcggccag acggcccagg cctttcaggt atccgatcag
      301 cttgacctgg ctgagatccc ccgccaggag ggcgtccggc tcccctcgac cccgcagggc
      361 gagacaggca acggtccatg gtccgatctc ggggatcgcc agcaggcgac ggtccccggc
      421 cggatccgac ggatcaaccc gcccggaagc aacctcccgc gcaaccttga ccatggctac
      481 ggctcgcttg ccgctgagac cgcaggcctc gagttcggct ggtgccgcgg cggagatgga
      541 ttggggcgag ggcaccgtcg caaggctcgc ttcctgccgg ccccggggcg aatgcggcag
      601 ccgccagtcc gttccggacg agctgaccgt cacgcccggg gaaaacttga tcccgaaccg
      661 ccggatcatc tgcctctgga tcctcgcggc acggcggtac tcgatcaact gctcggtgat
      721 cgcccagagc agggcctccc atggcaccgg ccgccgcatc gggcggttct ggaggcgccc
      781 gttgagggcc cggcccagca ggggatcctc ccggaaggcg ttgagaaacc cgaccaggtc
      841 gtcatcgacc ccgaggctga aacgcatccg gtcgaggccg gcctccagcg cttcctcgcc
      901 ggcctcccgc aaacccgccg atcccggccc ctccagcagc gccgggtcca ccggctcggc
      961 cgcgaagacg aactcgccgc ggtggccttc acgcaccctg gcaatcatcg gctggtcctc
     1021 gatcaccagc agtcgttcca gccagggacc gcgaagcctg aagaccagat cgccgccgac
     1081 cagccgcggc atccgcaagg gtgcattcgg ccggactgcg atctccgcga ccaggcccgt
     1141 gggtctacct ccccagaacg tggacgtcaa ccaggaagga gcggctggcg acgacgttcg
     1201 ccggacgggc gcgacggccc agcctgcggg ccttgcgctt cgaccctccg ctcaccgcac
     1261 gcacggcggc aacggtggca gcaccggcga tggcaccacc cgccaccgcg atggcggcag
     1321 tggtgacctc gttcttgagc gccggcagcg cgccgcccga ctcgtattcg cgaggcagtg
     1381 cccggacttc cccgccgctt tgcgacgcgg tgacgtcctc ctcagaaatc agttcgccat
     1441 caacctcttc agccacgacc cggaatctac ccgtgcgatc ggaggtaaca accgcctaac
     1501 ccgcaatttg cggggttagt tcaaggtctt ctcgtagacc cggaacttct tggtcacctc
     1561 ggcacccatg ccctccagg
//
LOCUS       DAOTIP010000561         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_93462_length_1578_cov_3.150361, whole genome shotgun sequence.
ACCESSION   DAOTIP010000561 DAOTIP010000000
VERSION     DAOTIP010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93462_length_1578_cov_3.150361"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..666
                     /locus_tag="P5138_09825"
     CDS             217..666
                     /locus_tag="P5138_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV60917.1"
                     /translation="MKRKLEWGYELDDDPARVDLDVICDFLGNEAYWSKGRSRELVEK
                     TFREASRVVGLYAPDGSTVGYCRAASDEVVFAYLCDVFVLESHRGRGLGKELVREMVD
                     GSPLKDLRWLLGTVDAHGMYRELGFRKPSFRIMERPGPKFAGDPPSE"
     gene            complement(684..>1578)
                     /locus_tag="P5138_09830"
     CDS             complement(684..>1578)
                     /locus_tag="P5138_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV60918.1"
                     /translation="FHVQRLKDKLGNKSNASSEVEFRGAWARMIGEDGRGIRTIIEMV
                     SHTRLDCAIGSSAGMRNAVAQATHHAMNRSAFGNKLIDQPLMENVLADLALESEAATA
                     SVMRLARAYDEAAAGDEGAALLQRLATPVLKYWICKRTPWHAVEALECFGGNGYAEES
                     GMPRLFRESPLMSIWEGSGNVQCLDALRAMVKSPASYEAFFNEVGEAAGADPRLDAFV
                     EKVQKSITEDPETLEIRARRVVESMGLAFQASMLVRHGDPAVADAFCASRLSGDWGEA
                     FGTLPAGTDFRTIIERSAPPV"
BASE COUNT          246 a          491 c          540 g          301 t
ORIGIN      
        1 acttcgacct tgagatggcc gttgtgctcc cgggtcacgg tgccgaccgc gttcagaagt
       61 gattcgacct tccgccattc gatcgaatgc ttctggtggt cgaagatctc ggcgagggtg
      121 ctgctgtgat ggttgctaag cccgtgatcg gccatcgggt tcctttcggt tcggtcaggg
      181 atgaatatca gtcctccacc tgcgagtctt tcccgggtga agcgcaaact cgaatgggga
      241 tatgaactcg acgacgaccc cgcgcgggtc gacctcgacg tgatctgcga cttcctgggt
      301 aatgaggcct actggtcgaa gggtcgttca cgggaactgg tcgagaagac cttccgcgag
      361 gcctcccgtg tggtcggtct ctacgccccg gacggttcga ccgtggggta ctgccgggcc
      421 gcctcggacg aggtcgtctt cgcctacctc tgcgacgtct tcgtgctgga atcacatcgg
      481 ggcagggggc ttggcaagga actggtgcgc gagatggtcg acggcagccc gctgaaggac
      541 cttcgctggc tgctcggcac cgtggatgcc cacgggatgt accgggagct cggcttccgc
      601 aagccctcct tccggatcat ggagcggcca ggcccgaagt tcgccgggga ccctccttcg
      661 gagtagatcc tgccggcgcc ggttcagacc gggggcgcgg agcgttcgat gatggtcctg
      721 aagtcggtgc cggcgggaag ggttccgaag gcctcgcccc agtcgccgga aaggcgagag
      781 gcgcagaagg cgtcggcgac ggccgggtcg ccgtggcgga ccagcatcga ggcctggaag
      841 gcgagcccca tgctttcgac cacccggcgg gcccggatct cgagcgtctc cgggtcctcg
      901 gtgatcgatt tctggacctt ctcgacgaag gcgtcaagcc gcgggtcggc gccggcagcc
      961 tcgccgacct cattgaagaa agcttcgtat gaagcggggc tcttgaccat cgccctcaga
     1021 gcgtcgaggc actggacgtt gcccgagccc tcccagatcg acatcagcgg actctcccgg
     1081 aagaggcgtg gcatgcccga ttcttcggcg taaccgttgc cgccgaagca ttccagcgcc
     1141 tccacggcat gccagggagt gcgcttgcag atccagtact tgagcaccgg ggtagccagg
     1201 cgctgaagca gggctgcccc ctcgtctccc gcggccgcct cgtcataagc tctggcgagt
     1261 cgcatcaccg aagcggtcgc agcttcggat tcgagtgcga ggtcggccag tacgttctcc
     1321 atcaggggct ggtcgatcag cttgttgccg aaggcggagc ggttcatcgc gtggtgggtg
     1381 gcctgcgcga ccgcgttgcg cattccggcg ctggagccga tcgcacagtc gagtctggtg
     1441 tggctgacca tctcgatgat cgtgcggatg ccgcggccgt cctcgccgat catgcgcgcc
     1501 caggcgccgc ggaactcgac ctcggaggag gcgttcgact tgttgcccag cttgtccttg
     1561 agccgctgga cgtggaag
//
LOCUS       DAOTIP010000562         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_93916_length_1573_cov_2.864954, whole genome shotgun sequence.
ACCESSION   DAOTIP010000562 DAOTIP010000000
VERSION     DAOTIP010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93916_length_1573_cov_2.864954"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="P5138_09835"
     CDS             <1..803
                     /locus_tag="P5138_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60919.1"
                     /translation="LYWAARGSGPGFFSVVTRYHLKLYPRPPVIANGIFTYPLESLEE
                     VFTWAKEIGPRIPRWMELMVFTHRDQGEPEVVVTAPVLAPSEPEAREALALLETCPAA
                     DRAKLSMPYFPARLEDLYAGVHASYPDEHRYAVDNMWTHAPAAELLPGIRRIAETLPE
                     APSHMLWMNWGTSPARQQMAYSVEDETYIALYAVWQDPADDRANIDWATGNMGAMESL
                     ASGIQLADENLGRRPARFVSEENLERLDRVRRARDPEGLFHEWMGRPV"
     gene            809..>1573
                     /locus_tag="P5138_09840"
     CDS             809..>1573
                     /locus_tag="P5138_09840"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60920.1"
                     /translation="MPQERPQPGEEEYLAELIRLKVEPAPLEVLLAAESGPLEPESSL
                     PRGEVDRLCDPAPLENPHYWSRMQDLSVRVSNETPMPDLSREMIEWWFDWHSRRSQRY
                     RVWHPVAHFANGRAPAAQPAVKPYWGVSNFPVEDVGDGAASIRIDFTSPREFGFTDDY
                     LDDDSVATIVCARVGDRLIEHTFMAHVFLRDGDGLRLRSHFWIAEKVRPRLPGPASVM
                     AGPIQSLVSNRYVRKVAVPDQVGRTLLVHCSEEYQHL"
BASE COUNT          253 a          550 c          520 g          250 t
ORIGIN      
        1 atctctactg ggcggcgcgg gggtccgggc ccggcttctt ctcggtcgtc acccgctacc
       61 acctgaagct ctatccgcgg ccgccggtga tcgccaacgg catcttcacc tacccgctcg
      121 agtccctcga agaagtcttc acttgggcga aggagatcgg gccgaggatc ccgcgctgga
      181 tggagctgat ggtcttcacc caccgcgacc agggcgagcc cgaagtggtg gtcacggccc
      241 cggtcctggc tccctcggag cccgaggcaa gagaggcgct cgccctgctc gagacctgcc
      301 cggctgccga ccgggcgaag ctctcgatgc cgtacttccc cgcccgactc gaggacctct
      361 acgccggcgt ccacgcctcc tacccggacg aacaccgcta cgcggtcgac aacatgtgga
      421 cccacgcgcc ggccgccgag cttctgcccg gcatccggcg gatcgccgag accctcccgg
      481 aggcgccgtc gcacatgctc tggatgaact gggggacgag cccggcccgg cagcagatgg
      541 cctactcggt cgaggacgag acctacatcg ccctctacgc ggtctggcag gatccggccg
      601 atgaccgggc caacatcgac tgggctacgg gcaacatggg agcgatggaa tccctggcca
      661 gtggcatcca gcttgccgac gagaatctgg gccgccgccc cgcccgcttc gtgagcgaag
      721 agaatctgga gcggctcgac cgggtccgga gggcccgcga tcccgaaggc ctcttccacg
      781 agtggatggg caggcccgtt tgaagccgtt gccacaggag cggccgcagc cgggcgagga
      841 ggagtacctg gcggagctga tccgtctgaa ggtcgaaccg gccccgctgg aggtcctgct
      901 cgcggccgaa tcgggtccgc tcgagcccga gtcttccctg ccccgggggg aggtggaccg
      961 tctctgcgac ccggcaccgc tcgagaaccc gcattactgg tcccggatgc aggacctgag
     1021 tgtcagggtc tcgaacgaga ccccgatgcc ggacctgagc cgggagatga tcgagtggtg
     1081 gttcgactgg cattcgaggc gaagccagcg ctaccgggtg tggcatccgg tcgcccactt
     1141 cgccaatggt cgggcgccgg cagcccagcc cgcagtgaag ccgtattggg gcgtaagcaa
     1201 cttcccggtc gaggacgtcg gcgacggggc ggcctcgatc cggatcgact tcacctcccc
     1261 gcgggagttc ggcttcaccg acgactacct cgacgacgat tcggtggcga ccatcgtctg
     1321 tgcccgggtt ggagaccgtc tcatcgagca caccttcatg gcgcatgtct tcctgcgaga
     1381 cggtgacgga ctccgtctgc gcagccattt ctggatcgcc gagaaggtcc gtccccgtct
     1441 gcccggaccg gcctcggtga tggccggacc gatccagtcc ctggtcagca accggtatgt
     1501 gaggaaggtc gcggtccccg accaggtcgg ccgcaccctc ctcgtccact gctcggagga
     1561 gtaccagcac ctc
//
LOCUS       DAOTIP010000563         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_93995_length_1572_cov_3.117996, whole genome shotgun sequence.
ACCESSION   DAOTIP010000563 DAOTIP010000000
VERSION     DAOTIP010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93995_length_1572_cov_3.117996"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1296)
                     /gene="coaBC"
                     /locus_tag="P5138_09845"
     CDS             complement(64..1296)
                     /gene="coaBC"
                     /locus_tag="P5138_09845"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRV60921.1"
                     /translation="MAKILLGVSGGIAAYKAVEFVRLATGAGHSVRVLMTASARKFVG
                     PDTFEGILGAPVLTSEFEKDPMRGSFPGEPMPEHDPIGHLAVAESADVFLVAPASANT
                     IAKLASGQADSILTTAFLAFEGPRLVAPAMNERMYLSQATQSNLSRLRELGVEVIDPG
                     EGRLASKGEAGVGRLPEPAELMERVEAALDHDRVKDLEGLRVLVTAGGTREGIDPVRY
                     IGNRSSGRMGLALAERATKRGAEVTVIAANVTLPAPAGVKRVDVESTVDLARACREQF
                     PAHDLLLMAAAPADFTVADTSNEKLSRTGGEFTLNLVPTEDILASLSANRIEGQTVVG
                     FAAQHGGDAIGRARQKLERKGADMIVLNDVSDKTIGFDSNDNAVTLVTGEGEQEVGKA
                     PKTEIADRILDRALQLRG"
     gene            complement(1338..1571)
                     /gene="rpoZ"
                     /locus_tag="P5138_09850"
     CDS             complement(1338..1571)
                     /gene="rpoZ"
                     /locus_tag="P5138_09850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRV60922.1"
                     /translation="MIKPRVDHLLQHSDSHYAAVVLAARRARQINSYYQSLSEGGYGD
                     YPPPMVPAEPGKGSLTLALEEVATGKLKYEYRS"
BASE COUNT          245 a          535 c          526 g          266 t
ORIGIN      
        1 cggagacggt ctccccgtcc accgagaccg aggcgccgga aacccgctgg accagggcgc
       61 gcatcagccg cgcaactgaa gcgcccgatc gaggatccgg tccgcgatct cggtcttcgg
      121 cgccttcccg acttcctgct cgccttcacc ggtgaccagg gttaccgcgt tgtcgttcga
      181 gtcgaagccg atcgtcttgt cggaaacgtc gttgagcacg atcatgtcgg cacccttgcg
      241 ctccagcttc tggcgggccc ggccgatcgc gtcgccgccg tgctgggcgg caaacccgac
      301 caccgtctgg ccctcgatcc ggttggcgga gagactggcg aggatgtcct cggtcgggac
      361 gaggttcagg gtgaactccc caccggtccg tgaaagcttc tcgttcgagg tatcggcgac
      421 cgtgaagtcg gccggggcgg ccgccatcag cagcagatcg tgggccggga actgctcccg
      481 gcaggcgcgg gcgagatcca cggttgactc cacgtcgacc cgcttcactc cggccggcgc
      541 cggcagggtc acgtttgcgg cgatcacggt tacctccgcc ccgcgcttcg tcgcccgctc
      601 ggcaagggcc agacccatgc ggccggagga acggttccct atgtagcgca ccggatcgat
      661 gccttcccgg gtgcccccgg ccgtcaccag aacccgcagg ccttcgaggt ccttgacccg
      721 atcgtggtcg agtgccgcct cgaccctctc catgagctcg gccggctccg gcaggcggcc
      781 aacccccgcc tcccccttcg aggcgagcct gccttcaccc ggatcaatga cctcgacccc
      841 gagctccctg agccgggaga ggttggactg ggtggcctgg gagaggtaca tgcgctcgtt
      901 catcgccgga gcgacgagac gcgggccctc gaaagcgaga aaggcggtgg tcaggatcga
      961 gtctgcctgg ccggatgcca gcttcgcgat cgtgttcgcc gacgccggtg caaccaggaa
     1021 tacgtccgcc gattcggcca ccgcgaggtg tccgatcgga tcgtgctccg gcatcggctc
     1081 ccccgggaag gacccccgca tcggatcctt ttcgaactcg gaggtgagta cgggggcgcc
     1141 gagaatcccc tcgaaggtgt cgggaccgac gaacttgcgg gccgaggcgg tcatcaggac
     1201 gcggaccgaa tgtccggccc cggttgcgag gcggacgaac tcgactgctt tgtaggccgc
     1261 gatccccccg cttacaccga gcaggatctt cgccatggtg ggttactccc gggtgggcag
     1321 tgtgcccggt ctcgaaccta cgagcggtac tcgtacttga gcttgccggt agccacttcc
     1381 tcgagcgcca gggtgagcga acccttgccc ggctcggccg gaaccatggg cggcgggtaa
     1441 tcgccgtagc cgccttcgct gaggctctgg tagtagctgt tgatctggcg ggcccgacgg
     1501 gcagccagaa ccaccgccgc gtaatgcgag tcggaatgct ggagaagatg gtcgacgcga
     1561 ggcttgatca tt
//
LOCUS       DAOTIP010000564         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_94011_length_1572_cov_2.578115, whole genome shotgun sequence.
ACCESSION   DAOTIP010000564 DAOTIP010000000
VERSION     DAOTIP010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94011_length_1572_cov_2.578115"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..380)
                     /locus_tag="P5138_09855"
     CDS             complement(69..380)
                     /locus_tag="P5138_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60923.1"
                     /translation="MAESGSDKSSGRGMPDGLKEAIEGAFAATERTRAQAQDLTEKTR
                     NRAQGLVDEVTRRGSDARGTLDGMRLVSRDEMHQLEERIEEISSRVAELERKSKSQPE
                     G"
     gene            455..1144
                     /locus_tag="P5138_09860"
     CDS             455..1144
                     /locus_tag="P5138_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60924.1"
                     /translation="MNPIEPAAGDAILVGDPRRAFALAQELTVQPKMSHQSRGLWGYV
                     GITSGGRRLTVQSTGSGGPSAIPVIGDLAAEGVGRMVRLGTCLATDPDLRAGTMILVE
                     RAVIADGAGRALSGNDDFASPDRRLHTALDGLGRDGTVSSHDLVERYEPEDRHAPEGG
                     PAIARDLQTAATLAMCRRLDIPAAAMLIVAEDRAGERLGEEDLWELFRSAGRALLSRL
                     DRLDPNPQVEV"
     gene            complement(1146..1487)
                     /locus_tag="P5138_09865"
     CDS             complement(1146..1487)
                     /locus_tag="P5138_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60925.1"
                     /translation="MTAESEGNDGIRDRVQSKGEKAAADLAQALIGSPAFGQAVSAAV
                     AARDRAADAQRAAMGAMGVSSQDEAERLERRVRVLSDRLEETEDRLDAALDQIRELRS
                     ELNDIGRKKRR"
BASE COUNT          234 a          549 c          486 g          303 t
ORIGIN      
        1 atccccagcg ggtggaaacc ccggctatga cgattcgctt gtcggtcaag ccggcctcag
       61 cttcaagtct aaccctcagg ttgagattta gacttgcgct ctaattcggc tacacgactg
      121 gaaatctctt cgattctttc ttccagttga tgcatctcgt cgcgactcac cagacgcatc
      181 ccgtccaggg ttccccgggc gtccgatccc ctccgggtca cctcgtcaac cagcccctgg
      241 gcccggttcc gggtcttctc ggtcagatcc tgggcctggg cccgggttct ttcggtcgct
      301 gcgaaggcac cttcgatcgc ttccttgagg ccgtccggca ttcctcgccc tgatgacttg
      361 tccgatccgc tttctgccat gggtctagtc tactttcaat cccgcccctc aattcgatcc
      421 gggcctttct ccggagccgc caatgccagt caagatgaac ccgatcgaac ctgctgccgg
      481 tgatgcgatc ctggtcggcg acccgcgccg cgccttcgcc ctggcccagg agctgaccgt
      541 gcagccgaag atgagccacc agtcgcgagg gctctgggga tacgtgggca tcacctccgg
      601 cggccggcgt cttacggtcc agtccacggg atcgggaggc cccagcgcca tccccgtgat
      661 cggggatctg gctgcggaag gtgtcggaag gatggtccgg ctgggcacct gcctcgcgac
      721 cgatccggac ctgcgggccg gaaccatgat cctcgtcgag cgcgcggtca tcgccgacgg
      781 ggccggccgg gcattgagcg gaaatgatga ttttgccagt cccgaccgcc ggctccacac
      841 cgccctcgac gggctcggcc gcgacgggac cgtctccagc catgacctgg tcgagcgata
      901 cgaaccggaa gaccgtcacg cgccggaggg cggaccggcg atcgccaggg acctccagac
      961 ggcggcgacc cttgccatgt gccgccggct cgatattcct gctgcggcga tgctgatcgt
     1021 ggccgaggat cgcgccggcg agcgtctcgg cgaggaggat ctctgggaac tcttccgttc
     1081 ggccggccgg gcgctactca gccgtttgga ccgactggac cctaaccctc aagtagaggt
     1141 ttagattagc gccttttctt gcgcccgatg tcgttcagct cggacctgag ctcccgaatc
     1201 tggtcaagcg cagcgtcgag ccggtcctcg gtctcctcca gacggtcgga aagcacccgg
     1261 acccgcctct cgaggcgctc tgcctcgtcc tgggaggaga cccccatcgc gcccatcgcg
     1321 gcgcgctggg cgtccgccgc ccggtctctg gcagccactg ccgccgacac ggcctggccg
     1381 aaggcgggac tgccgatcag ggcctgcgcc aggtcggcgg cggccttctc ccccttcgac
     1441 tggacacggt ctctgattcc gtcgttgcct tcgctttcag cggtcatggc acgatcttaa
     1501 agggcgcctc gaaggaagtg tcaaggtgct ggcgaaaccc gttgcgatac ggttcccctg
     1561 ccgcgtcacg gg
//
LOCUS       DAOTIP010000565         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_94692_length_1564_cov_3.135852, whole genome shotgun sequence.
ACCESSION   DAOTIP010000565 DAOTIP010000000
VERSION     DAOTIP010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94692_length_1564_cov_3.135852"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5138_09870"
     CDS             complement(<1..325)
                     /locus_tag="P5138_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60926.1"
                     /translation="MGRIGGRFVVCLTVLAIAGCSGAAGVHAESQAAPEVRNHSPASA
                     SAYRGHKFNLQQAGCYDGGQIKALPPLKYQVNSWYYGHPDHYYEKIWWRAKISHGKSP
                     PARAMR"
     gene            435..>1564
                     /locus_tag="P5138_09875"
     CDS             435..>1564
                     /locus_tag="P5138_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRV60927.1"
                     /translation="MDLLLVPLDDAIVFPSVTATLQIDVGDEEQVFLLSRRDGEFARV
                     GVIADVVEQGQSPRGETVATVVGLRRGLAGIAHPDEVDPDALRIEVQEIHDGHPEDEH
                     TKELVREYRAIVEEILELRNADSRITAFLRNVTEPGELADTSGLSPDLSFEQKFELLD
                     TLNVTARLEKAVEFQRERLTELQVRSKIRDDVESGAQQQQREYFLREQMKSIRKELGE
                     DEGDVIEEYEQKIAEAEMPEAVQEQAVKELRRLERQGEQSGEGSMIRSYLDWLIAVPW
                     SKRSDERLDPEYTREVLDADHAGLDDVKRRITEFVAVRKLRQERESEADSRADGAILT
                     LVGPPGTGKTSIGESVAKALGREFVRISLGGVHDEAEIRGHRR"
BASE COUNT          303 a          497 c          514 g          250 t
ORIGIN      
        1 ccctcatcgc tcgggcgggt ggggacttcc cgtgggagat ctttgcccgc caccagatct
       61 tttcgtagta gtggtcgggg tgtccgtagt accaggaatt cacctggtac ttgagcgggg
      121 ggagcgcctt gatctggccg ccgtcgtagc agccggcctg ctgcaggttg aacttgtgtc
      181 ctcggtaggc cgaggcggaa gccggcgaat ggttccggac ttcgggagcc gcttgcgatt
      241 cggcatggac gccggcggca ccggaacacc cggcgattgc aagaaccgtc agacagacga
      301 cgaaccttcc cccaattcgt cccatgaacc agccataacc gatcgagtcg ccggcgtcaa
      361 cccgcaggtg gttgacagcg caactaagtg cgctatgctt acggcaggac taactacaga
      421 aactggactt actaatggat cttctgctgg ttccactcga tgacgcaata gtttttccct
      481 cggtcacggc gacgcttcag attgacgtcg gtgacgagga acaggtcttc ctgctctcgc
      541 gccgcgacgg cgaattcgcc cgcgtgggcg tgatcgccga cgtggtcgaa cagggtcagt
      601 cgccccgggg cgagacggtc gccaccgtgg tcggcctccg ccgtgggctg gccggcatcg
      661 cccaccccga cgaggttgac cccgacgccc tccggatcga ggtccaggag atccacgacg
      721 gccatcccga agacgagcac acgaaggaac tggtccgcga ataccgggcg atcgtcgagg
      781 agattctcga gctcagaaac gccgacagcc ggatcaccgc cttcctgcgc aacgtgaccg
      841 agccgggcga gctggccgac acttcgggtc tcagccccga cctcagtttc gagcagaagt
      901 tcgagctgct cgacacgctc aacgtgaccg cccgcctcga gaaggcggtc gaattccagc
      961 gcgagcggtt gaccgaactc caggtccgca gcaagatccg cgatgacgtc gagtcgggcg
     1021 cgcaacagca gcagcgcgag tacttcctgc gcgagcagat gaagtcgatc cgcaaggagc
     1081 tcggcgaaga cgaaggcgac gtgatcgagg agtacgagca gaagatcgcc gaggccgaaa
     1141 tgcccgaggc ggtccaggag caggcggtca aggagctccg ccggctcgaa cgccagggcg
     1201 agcagtcggg tgaaggctcg atgatccgtt cctacctgga ctggctgatc gcggtgccct
     1261 ggagcaaacg ctccgacgag cggctcgatc ccgagtacac ccgggaagtc ctcgacgccg
     1321 accacgccgg tctcgatgac gtcaagcgcc ggatcaccga gttcgtagcc gtgcgcaagc
     1381 tgaggcaaga gcgtgaatcg gaagctgaca gccgggccga cggcgcgatc ctgaccctgg
     1441 tcggaccgcc cggcaccggc aagacctcga tcggcgaatc ggtcgccaag gccctgggtc
     1501 gcgagttcgt ccgtatctcg ctgggcggcg tccatgacga ggcggagatc cgcggtcacc
     1561 gtcg
//
LOCUS       DAOTIP010000566         1560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95063_length_1560_cov_2.741528, whole genome shotgun sequence.
ACCESSION   DAOTIP010000566 DAOTIP010000000
VERSION     DAOTIP010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95063_length_1560_cov_2.741528"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1007)
                     /gene="coxB"
                     /locus_tag="P5138_09880"
     CDS             complement(192..1007)
                     /gene="coxB"
                     /locus_tag="P5138_09880"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRV60928.1"
                     /translation="MTRLPILKMVLAVIVFGTVQSVILLQFNWFGDEASTAAKPIDTL
                     LDVTIIISSFIFAIVCVALGYALIKWRAKPGDESDGLPIHGNTRLEIIWTIIPVIIVL
                     GLAGYSWAVLDDIEKTDSNALHINAYAQQFAWTFGYPEDGNKWSEGELHVPVDRQVIF
                     DLQAQDVIHSFWVPEWRIKKDAVPGLETEVIVTPDKEGTYSLICAELCGLGHTTMRAT
                     VVVESEAKYKAWLNKQTTQVPEDILKTTNQYEADQLKKEEQSGGIEGSGVIEQ"
     gene            complement(1060..>1560)
                     /locus_tag="P5138_09885"
     CDS             complement(1060..>1560)
                     /locus_tag="P5138_09885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV60929.1"
                     /translation="DEEPLSYLTSFFAHPPVGYDEAGIAAAGSISFAGRLEHDEVARV
                     VPASDSMIVPSTFPEAFGMVAAEAAASGVLPVCAEHSGLAEVTGTLAEEVPEVKSMIG
                     LPLGPGAVAELAGKVNRWLALDRPERMRIGKNIAESADRNWSWQGVARDVISASAGQV
                     RKVERT"
BASE COUNT          289 a          511 c          461 g          299 t
ORIGIN      
        1 cgatgacctg cggatagccg gtctgctcgg tctggaaggc atcgagaccc gaaagcaggc
       61 gcaatccgac cacgagacag aaaccgaagg ccgctccgag caccagcccg atcgcacccc
      121 tgtaccaacc tggttgcctg attgccgctg ccattacttg acctccatca tcaggttgcg
      181 gagcgaggtg gtcactgttc gatgaccccg cttccttcaa tgcccccgga ctgctcttcc
      241 ttcttcaact gatctgcttc gtactggttc gtcgtcttaa gaatatcttc cgggacctgc
      301 gtggtctgct tgttgagcca ggctttgtac ttcgcctcgg actcgaccac caccgtggcc
      361 cgcatggtcg tgtgaccgag accgcagagc tcggcacaga tcagcgagta cgtgccctcc
      421 ttgtcgggcg tcacgatcac ttcggtctca aggcccggca cggcgtcctt ctttattcgc
      481 cattccggca cccagaacga gtggatcacg tcctgcgcct gaaggtcgaa gatgacctgc
      541 cggtcgaccg gtacatgcag ttcgccttcg ctccacttgt tgccgtcctc cgggtacccg
      601 aaggtccagg cgaactgctg ggcgtaggca ttgatgtgaa gcgcgttgct gtcggtcttc
      661 tcgatgtcat cgagaaccgc ccacgagtag ccggccaggc cgagcacgat gatcaccgga
      721 atgatcgtcc agatgatctc gagccgcgtg ttgccgtgga tcggcagacc gtcgctttcg
      781 tcgcccggct tggcccgcca cttgatcagg gcgtagccga gggcgacaca gacgatcgcg
      841 aagatgaagg aggaaatgat gatggtgaca tcgagcagcg tgtcgatcgg cttggccgcg
      901 gtcgaagctt catcgccgaa ccagttgaac tggagcagga tgactgactg gaccgtgccg
      961 aagacgatta cggccagcac cattttcaaa atcgggagac gagtcatgag gcgcccgcca
     1021 ttctattgga gccggggacg aatgctctct gacccccgct cacgtgcgct cgacttttcg
     1081 aacttggcct gctgatgcgg aaatcacgtc cctcgccacc ccctgccagc tccaattcct
     1141 atcggctgac tcggcaatgt ttttaccgat ccgcatcctc tccggccgat ccagagccag
     1201 ccagcggttg accttgcccg ccagctcggc gaccgctccg gggccgagcg gcagcccgat
     1261 catcgatttg acctccggaa cctcctcggc cagggtgccc gtcacctcgg ccagcccgga
     1321 gtgctcggcg cagaccggca gcaccccgga ggctgcggcc tcggccgcaa ccatgccgaa
     1381 cgcctcgggg aaggtcgaag gcacaatcat cgagtcggag gcgggaacca ctcgggccac
     1441 ctcgtcatgc tcgagccggc cggcgaagga aatcgatccg gccgccgcta tccccgcctc
     1501 gtcgtagccg acaggagggt gggcgaagaa cgaggtcagg taggaaagcg gttcctcgtc
//
LOCUS       DAOTIP010000567         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95320_length_1557_cov_2.448069, whole genome shotgun sequence.
ACCESSION   DAOTIP010000567 DAOTIP010000000
VERSION     DAOTIP010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95320_length_1557_cov_2.448069"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1557
                     /locus_tag="P5138_09890"
     CDS             46..>1557
                     /locus_tag="P5138_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60930.1"
                     /translation="MIDDLPGSQEGPTKISVRGLTGDEAKLLDARLPPPEGSRNKPAS
                     KGTHRWAMELPRSVRSDPRFAMGTAARFPSWLELADGPTMLVVTVISPIMNELATVFR
                     IREGAGPKTLKGAKKAVERSIAAHRMLGLDWRPIKDLLDPTLDAEAVIAARRRLIEGW
                     AEPATDPGEGAMAFLCSRLAHSYYGKARKEGTAIRSQVINSKTEPVLLGTLQTWDSLL
                     DYLGEHISDVDSATAAPISTRIPAGPPPEVGERIQLARRWFVEVFDSAHTSQEPGNPE
                     LWGLVPPSHASNWDEDIGDLRGEAGESGGLAQIEVESSEPIEPLPLSTDVVRSGSSEE
                     IAIPDTEDASTAARGFDSNLWLTQLSPGMADSIWELWGTTVIARHPDVLVDCSYPEVT
                     FAGILQPAAMVWEDIALTCWFLCFGPYSRTTLDGLEARLDKSLEDLSDLGCPVDRQVF
                     RDLIAAGEGKDWLFAEGLVVSITIGSNVTANDDKPDADEVFVRLRDIVSRHRKA"
BASE COUNT          281 a          507 c          499 g          270 t
ORIGIN      
        1 cgtattgtca gctccgtcct ctccccagtc ctcgcgggaa tccaggtgat tgacgatctg
       61 ccgggttcgc aggagggtcc caccaaaatc tccgtccggg gattgaccgg cgacgaggcg
      121 aagctactgg acgcgaggct tccgccgcca gagggcagtc gcaacaagcc ggcgagcaag
      181 ggaactcatc gctgggcgat ggaactgccg cgctcggtgc gatcggatcc ccggttcgcg
      241 atgggcaccg ccgccagatt tccttcatgg ctcgagctgg cggacggtcc gacgatgctc
      301 gtggtcacgg tcatctcacc gatcatgaat gagctggcga cggtgtttcg catccgcgag
      361 ggcgcgggcc ccaagaccct gaagggcgcg aagaaggccg tggagagatc catcgcggcg
      421 cacaggatgc tcggactcga ctggcggccg atcaaggacc tgctcgatcc gaccctcgac
      481 gccgaggcag tcatcgccgc gaggcgccgg ctgatcgagg gctgggctga gccggccacg
      541 gatcccgggg agggcgccat ggcttttctc tgctcccgcc tcgcccacag ctactacggc
      601 aaggcgcgaa aagaggggac ggcgatccga tcccaggtca tcaactcgaa gaccgaaccg
      661 gtgttgctcg ggaccctaca gacctgggat tcgttgctcg attacctggg cgagcacatc
      721 tcggacgtcg actcggccac cgcagctccg atctcgacga ggatccctgc cggccctcct
      781 cccgaggtcg gggaacgcat ccagctggct cgcagatggt tcgtcgaggt gttcgactcg
      841 gcccatacat cgcaggaacc cgggaacccc gaactgtggg gactcgtccc cccatcgcac
      901 gcgagcaact gggacgaaga catcggcgat cttcgtggcg aagccggaga gtcgggcggc
      961 ctcgcccaga tcgaagtcga atcatcggaa ccgatcgagc ccttgccgct ttcgacggat
     1021 gtcgtccggt ctggttcgtc ggaggaaatc gccatcccgg acacggaaga cgcatcaacg
     1081 gcggccaggg gattcgactc gaacctctgg ctcacccagc tctcgccggg catggccgac
     1141 tcgatctggg aactctgggg caccacggtt atcgctcgcc atccggatgt cctcgtcgac
     1201 tgctcttatc cggaggtcac cttcgcgggc attttgcagc cggcggccat ggtctgggaa
     1261 gacatcgccc tcacatgctg gtttctgtgt ttcggcccct actcgcgaac caccctcgac
     1321 ggcctcgagg cccggctcga caagtccctc gaagaccttt ccgacctcgg atgcccggtg
     1381 gaccgacagg tctttcgcga cctgatcgcg gcgggcgagg gcaaggactg gctgtttgca
     1441 gagggactcg tggtcagcat caccatcggt tcgaatgtca ctgcgaatga cgacaagccg
     1501 gacgccgatg aggtgttcgt ccggttgaga gacatcgtct cccgacaccg gaaggcg
//
LOCUS       DAOTIP010000568         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95559_length_1554_cov_3.375584, whole genome shotgun sequence.
ACCESSION   DAOTIP010000568 DAOTIP010000000
VERSION     DAOTIP010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95559_length_1554_cov_3.375584"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..270)
                     /locus_tag="P5138_09895"
     CDS             complement(40..270)
                     /locus_tag="P5138_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HRV60931.1"
                     /translation="MAADLNELKGMIESALPGSTAEVIDETGTGDHLRAEVSAPQFEG
                     RSRIDQHRLVKAAVRERFDDGTIHALTIKILD"
     gene            complement(270..>1554)
                     /locus_tag="P5138_09900"
     CDS             complement(270..>1554)
                     /locus_tag="P5138_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRV60932.1"
                     /translation="GLVRGGVAPDHPKIKSVTRVYERTAAKEGFRFFGNVEIGRDLNV
                     EDFENHYHAILYTFGAEADRELGIPGEDLPGSFAATAFVGWYNAHPDYADRDFKLDQA
                     KRAVVIGNGNVALDVARMLALDDAELRKTDTADHAIELLDASTIEEIVVLGRRGPAQA
                     AYTNPEVKELGELEEADVIVNPDEVELDLASQAWIESDEADKTARVNVEIVKEYASRQ
                     PEGKPKRIVLRFLASPVEIKGGSKVESIVIGHNELVEQEGSLRARDTGEREELECDLI
                     LRSVGYTGVPLRGVPFDERRGTILNDGGRVLESHGGEPRTGHYTAGWIKRGPSGVIGT
                     NKKCATETVAHLFEDVAAGSLLEPANPEPAAIEELLAERGANFVTFSGWEKIDAAEVG
                     KGEPHGRPRVKFVRIDEMLETAAEQAEAGAAAGGE"
BASE COUNT          210 a          506 c          523 g          315 t
ORIGIN      
        1 gatcagccat gggttgatgg taaaggatgg tccaggccgt cagtcgagga tcttgatggt
       61 cagggcgtgg atggtgccgt cgtcgaaccg ttccctcacg gccgctttga ccagccggtg
      121 ctggtcgatc cggctcctgc cctcgaactg cggagcggag acctcggccc gaaggtggtc
      181 acctgtgccg gtctcgtcga tcacttcggc ggttgatccg ggcagggccg actcgatcat
      241 gcccttcagc tcgttcaggt cggcggccat cactcgcctc cggcggcggc acccgcctcg
      301 gcctgttcgg ccgcggtctc gagcatctcg tcgatccgca cgaacttgac tctcgggcga
      361 ccatggggct cgcccttgcc gacctcggcc gcgtcgatct tctcccagcc gctgaaggtg
      421 acgaagttcg ccccgcgttc ggccagcagt tcctcgatcg cggcaggttc cgggttcgcc
      481 ggctcgagca gactgcccgc agcgacgtct tcgaagaggt gggcgaccgt ctcggtcgcg
      541 cacttcttgt tggtgccgat cacaccggaa ggcccgcgct tgatccagcc ggccgtgtag
      601 tggccggtgc ggggttcgcc cccgtggctt tcgagcaccc gtccaccgtc attgaggatg
      661 gtgccgcgcc gctcgtcgaa gggcaccccg cgcagcggaa cgccggtgta gccgaccgat
      721 cgcaggatca ggtcgcattc gagctcttcg cgctcgccgg tgtcgcgggc ccggaggctg
      781 ccttcctgtt cgaccagttc gttatggccg atgacgatgc tctctacctt gctgccgccc
      841 ttgatctcga ccggtgaggc gaggaacctg agcacgatgc gcttcggctt gccctctggc
      901 tggcgggagg cgtactcctt gacgatctcg acgttgaccc gggcggtctt gtcggcttcg
      961 tcggattcga tccaggcctg acttgccagg tccagctcga cttcgtccgg gttgacgatt
     1021 acgtcggctt cctcgagttc gccgagttcc ttgacttcgg ggttcgtgta ggcggcctgg
     1081 gcgggccccc ggcggcccag cacgacgatc tcttcgatgg tcgacgcgtc gagcaactcg
     1141 atcgcgtggt cggcggtgtc ggtcttgcgg agctcggcgt catcgagggc gagcatcctg
     1201 gcaacgtcaa gggcaacgtt gccgttgccg atcaccacgg cgcgtttggc ctgatccagc
     1261 ttgaaatcac ggtcggcgta atccgggtgt gcgttgtacc agccgacgaa ggcggtggcg
     1321 gcgaagctgc cgggcagatc ttcgccgggg atgcccagct cgcggtcggc ctctgccccg
     1381 aaggtgtaga ggatcgcgtg gtagtggttc tcgaagtcct cgacgttcag gtcgcggccg
     1441 atctcgacgt tgccgaaaaa gcggaatccc tccttggccg cggtgcgctc gtagacccgg
     1501 gtcaccgact tgatcttggg atggtcggga gcgacgccgc cacgcaccag cccg
//
LOCUS       DAOTIP010000569         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95566_length_1554_cov_3.060040, whole genome shotgun sequence.
ACCESSION   DAOTIP010000569 DAOTIP010000000
VERSION     DAOTIP010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95566_length_1554_cov_3.060040"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="P5138_09905"
     CDS             <1..376
                     /locus_tag="P5138_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60933.1"
                     /translation="NRSFGPAQTFSTTARKLKLKLNRYRVRFSRRGATVKMTASLGPC
                     PAVTWQTVRFERKVRKGKRRVWKKIGSKMVSGGKCQATMKVQRLKSKTSLRVRALSIN
                     GYKWANSSVRTVKVSGANRSRR"
     gene            451..524
                     /locus_tag="P5138_09910"
     tRNA            451..524
                     /locus_tag="P5138_09910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Gly,seq:gcc)
     gene            604..>1554
                     /locus_tag="P5138_09915"
     CDS             604..>1554
                     /locus_tag="P5138_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRV60934.1"
                     /translation="MEPAARRHRKPHRPAYHPRSHARRQRGSGLLGLREASAMAIGGM
                     IGGGIFSVLGVTIDLAGHMAFAAFLIGGLIAALTAHALARLAVHADRPGGPFTYLREE
                     GHGKAAAWVAWLLIVGYVFALAVYAFTFGHYLAHVLGATELVARVSSIGILLLFVIVN
                     LRGVTTSGLTEDIVVVAKLAVLATFTVIGILMFSTDRLSPLDNDGLIGVFLGASVIFV
                     AYEGFELVPYDYDDISEPRRTVPLALYISVGVVTLVYVAVTLASQMLLSDGAIIAEKE
                     VAFALVGKAALGTAGLWAATLGAVLSTSSAINATLFSTARL"
BASE COUNT          257 a          530 c          488 g          279 t
ORIGIN      
        1 gaaccgctcc ttcggcccag cccagacgtt ttccacgacc gcccggaagc tgaagctcaa
       61 gctgaaccgg taccgggtca ggttcagccg caggggagcg accgtgaaaa tgaccgcgtc
      121 tctcgggcca tgccctgccg tcacctggca gaccgtccgc tttgaacgga aggtcaggaa
      181 gggcaaacgg cgggtctgga agaagatcgg ctcgaagatg gtcagcggcg gcaagtgcca
      241 ggccacgatg aaggtccagc ggttgaagtc aaagacgagc ctgcgggtcc gggcactctc
      301 gatcaacgga tacaagtggg ccaattcgtc ggtccgcacc gtgaaggtga gcggagcgaa
      361 ccgaagccgc cgctagcggc caccgccgcg acggcggacc ttcgcgacga aacgccgtga
      421 aggcccgccc ggatatactg cgccgtctcc gcggctgtag ctcagttggc tagagcgtct
      481 gcttgccatg cagaaggtcg agggttcgaa tcccttcagc cgcttcattc ccggaccaag
      541 cgcaggaggg tttgatccgg agcgttcaag gccagtccgg ccgcgcattg gatacttccg
      601 gtcatggagc ctgcggcccg cagacaccga aaaccgcacc gacctgccta ccatccccgc
      661 agtcacgccc ggcgccagcg tggaagcggc ctgctcggcc tccgcgaggc cagcgcgatg
      721 gcgatcgggg gcatgatcgg tggcgggatc ttcagcgtcc tcggggtcac catcgacctg
      781 gccggccaca tggcattcgc cgccttcctg atcggcggac tgatcgccgc cctgaccgcc
      841 cacgccctgg cgcgactggc cgttcacgcc gacaggccgg ggggaccatt cacctacctt
      901 cgcgaagagg gccacggaaa ggccgcagcc tgggtggcgt ggctgctgat tgtcggctac
      961 gtgttcgccc tcgccgtcta tgccttcacc ttcgggcatt accttgcgca cgtgctcggc
     1021 gcgacagagc tggtcgcccg ggtttcctcg atcggcatcc tgctgctctt cgtgatcgtg
     1081 aacctgcgcg gggtcaccac ctcgggcctg accgaggaca ttgtcgtcgt cgccaagctc
     1141 gcggtgctcg caaccttcac agtcatcggg atcctcatgt tctccactga ccggctgagc
     1201 ccgcttgata acgacggcct gatcggcgta ttcctgggag cctcggtgat cttcgtcgcc
     1261 tacgagggct tcgagctggt gccctacgac tacgacgaca tctcggaacc acggcggacc
     1321 gttccccttg ccttgtacat ctctgtgggg gtcgtgaccc tcgtctatgt cgccgtcacg
     1381 ctcgcttcgc aaatgctgct ttcggacggc gcgatcatcg ccgagaagga ggtcgccttc
     1441 gccctggtcg gcaaggcggc cctcggcacc gccggcctct gggcggcaac ccttggcgcc
     1501 gtgctctcca ccagttcggc catcaacgcg acactgttct cgaccgcccg tctg
//
LOCUS       DAOTIP010000570         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95713_length_1552_cov_3.255845, whole genome shotgun sequence.
ACCESSION   DAOTIP010000570 DAOTIP010000000
VERSION     DAOTIP010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95713_length_1552_cov_3.255845"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="P5138_09920"
     CDS             <1..594
                     /locus_tag="P5138_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRV60935.1"
                     /translation="PTPVVGMVGELPDPAHAAGENWREGDSIALVGPFDPSLAGSELE
                     KQRGQLGKGLPQIEIAPVREAIELVREAVRSGRVRTATDVSDGGITTALAELAIGSGL
                     GCDVHLTAVLAKRDCSAEDALFGEGPGGFILAGDEAELEKLGGHGVEFEVIGEVRGNE
                     LIVTAADRRVEILVEEAEAAFESLEDRIESDQAGLTH"
     gene            705..>1552
                     /locus_tag="P5138_09925"
     CDS             705..>1552
                     /locus_tag="P5138_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRV60936.1"
                     /translation="MRQFLATESGSAGLLLLAAVAALVWSNAPFADTYDSFWGTSVSI
                     NIGDFSIAHDLRFWIDEGLMALFFFVIGMEVRREMAMGELTDRKRVLAALLGGIGGMV
                     APALIYLAINSGSGHEHGWGVVIATDTAFVLGALALVGPRFPTQLRVFLLTLAIVDDI
                     VAITVLGLFYSESLDSTALAICVAALVLIWLFPRIKVWRSGAYLVAGTVAVVAAMESG
                     FHATLAGMIAGLLVAAYPPQPKKVEKAASRVRAFRQSPLPEVARSANLSVAQALSPNE
                     RFQTVL"
BASE COUNT          230 a          540 c          522 g          260 t
ORIGIN      
        1 cccaccccgg tggtcggcat ggtcggagag ctgcccgatc cggcccacgc cgccggggag
       61 aactggcgcg aaggcgactc gatcgccctg gtcgggccgt tcgatccgtc actcgccggc
      121 tccgaactcg agaagcagcg cggccagctc ggcaaggggc tgccccagat cgagatcgcg
      181 ccggtccggg aggcaatcga actggtccgc gaggcggtcc gctccggccg ggtccgcacc
      241 gccaccgatg tgtctgacgg cggcatcacg accgcattgg ccgagctggc gatcggctcc
      301 ggcctcggct gcgacgttca cctgaccgcg gttctcgcca agcgggactg ctcggccgag
      361 gacgccctct tcggcgaggg cccgggaggt ttcatcctgg ccggtgacga agccgagctg
      421 gagaagctcg gcggccatgg agtcgagttc gaggtgatcg gcgaggtccg cgggaacgag
      481 ctgatcgtca ccgccgccga ccgccgggtc gagatcctgg tcgaggaagc cgaagcggca
      541 ttcgaatccc tcgaagaccg catcgagtca gaccaggccg gcctgacgca ttaggggccg
      601 gacagggtca acccgatagc tttgctgggg acttggcccg gcctccgcac gatcccgaca
      661 caaccgagag cagacgtgat cttgcggcgc aggtcgcgaa gccgatgcgc cagtttctgg
      721 cgaccgaatc gggtagcgcc ggacttctgc tcctcgccgc cgtggcagca ctggtctggt
      781 cgaacgcccc cttcgccgac acctacgaca gcttctgggg cacctccgtc tcgatcaaca
      841 tcggtgactt ctcgatcgcc cacgacctga ggttctggat cgacgagggg ctgatggcgt
      901 tgttcttctt cgtgatcggc atggaggtca ggcgcgagat ggcgatgggc gagctgaccg
      961 accgcaaacg ggttctggcc gctctgctcg gcgggatcgg gggcatggtc gcgccggccc
     1021 tgatctacct cgcgatcaac tccggctccg gtcacgagca cggatggggc gtggtcattg
     1081 ccaccgacac ggccttcgtg ctcggggccc tggccctggt cggcccgaga ttcccgaccc
     1141 agctccgggt cttcctgctt accctcgcaa tcgtcgatga catcgtcgcg atcaccgtcc
     1201 tcggcctctt ctactcggag tcgctcgact ccaccgcgct cgccatctgc gtggcggcgc
     1261 tggtgctgat ctggctgttc ccccggatca aggtctggcg ttcgggcgcg tacctggtcg
     1321 ccggcacggt cgcggtggtg gcggcgatgg aatccggctt ccacgccacc ctggccggga
     1381 tgatcgccgg gctcctcgtc gccgcctacc cgccgcagcc caagaaagtc gaaaaggcgg
     1441 ccagccgggt ccgcgccttt cgccagtcac cgctgccgga ggtcgcccgg tcggcgaacc
     1501 tcagcgtggc tcaggccctc tccccgaacg aacggttcca gacggtcctg ca
//
LOCUS       DAOTIP010000571         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95796_length_1551_cov_3.578877, whole genome shotgun sequence.
ACCESSION   DAOTIP010000571 DAOTIP010000000
VERSION     DAOTIP010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95796_length_1551_cov_3.578877"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="P5138_09930"
     CDS             <1..505
                     /locus_tag="P5138_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60937.1"
                     /translation="LLFVGVVMVGYGITALWFGMRDVMDVGGYCAEGGPYVIQQHCPD
                     GAEVLMLTGIPIGIIGLFVAMAGAAKSASGAMGLLLLGWPAIFVSLGYNFIYYAINPP
                     EGMGGTAGWWVCGIIFALMGLPALAAVPMLVKAIQPGRRYAVLTVFTLAALVGVIVGI
                     QIANSVD"
     gene            complement(548..>1551)
                     /locus_tag="P5138_09935"
     CDS             complement(548..>1551)
                     /locus_tag="P5138_09935"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV60938.1"
                     /translation="KWSERDHAEAEGALAAPVMVRGRNWGRVIAIQTDSEFDRFTPIA
                     LERAAVAVGLGLMRGGEEAELRARSRGNLLFELAAGLMEPLAAARRASTLGFPRRHGQ
                     LVPIAAVWRPEAVEPGVGYEEAWAPLLPAIRAAMAEPDRPALLGSRGNCVLGVLDRGT
                     LGSEPDDVASRERLARRFAGVLNRHEIDPESVALAIGGAAGTWEELAPALVRARFAAE
                     AGAAGPVRSWHDASRTEIGQLLLSMRGSPELLQFSDERLGPLLDLPGERGKDLLDTLE
                     VYLHSGGRKTEAATELEIERQSLYHRLSRIEEVLDVDLKDGDTALALHLAVMIRRLL"
BASE COUNT          233 a          548 c          499 g          271 t
ORIGIN      
        1 gctgcttttc gtcggcgtcg tcatggtcgg gtacgggatc accgctctct ggttcggaat
       61 gcgcgacgtc atggacgtcg gaggctattg cgccgagggt gggccctacg tgatccagca
      121 acactgtccc gacggcgcag aagtgctgat gctcaccggc atcccgatcg ggatcatcgg
      181 gctcttcgtg gcgatggccg gggccgccaa gtcggccagc ggcgccatgg gcctcttgct
      241 gctcggctgg ccggcgatct tcgtttccct cggctacaac ttcatctact acgcgatcaa
      301 cccgccggag gggatgggcg gcaccgccgg atggtgggtc tgcggcatca tcttcgccct
      361 gatgggcctt cccgcccttg ccgcggttcc gatgctggtc aaggcgatcc agcccggtcg
      421 tcgctatgcg gttctgaccg tgttcaccct cgccgccctg gtcggcgtga tcgtcggcat
      481 ccagatagcc aactccgtcg actgaacgga ttccggtcgg cggcaatcac cgtacgttta
      541 ggtctgttca gagcagacga cgaatcatga ccgccaggtg aagggccagc gcggtgtcac
      601 cgtccttgag gtcgacgtca aggacctcct cgatacgcga gaggcgatgg tagagcgact
      661 gtcgttcgat ctcgagttcg gttgccgctt ccgtcttcct gccaccgctg tggagataga
      721 cctccagggt gtcgagcagg tccttgcccc tctccccggg tagatcgagc aacggtccca
      781 ggcgttcgtc gctgaactga aggagttccg ggctgccacg catcgaaagc agtaactggc
      841 cgatctcggt tcggctcgcg tcatgccagg aacggacggg gccggcggcg cctgcttctg
      901 ccgcgaatct cgccctgacc agggccggcg ccagctcctc ccaggtgccg gccgccccgc
      961 cgatcgccag ggcgaccgac tccggatcga tctcgtgccg gttcagcacc ccggcgaatc
     1021 gccgggccag ccgctccctc gacgccacgt cgtcgggttc cgaaccgaga gttccccgat
     1081 cgagaacccc gagtacgcag ttaccccggg aaccaaggag agccgggcgg tcaggctcgg
     1141 ccattgccgc ccggatcgcc ggcagcagcg gggcccacgc ctcctcgtac ccgaccccgg
     1201 gttcgacggc ctccgggcgc cagacggcgg cgatcggcac cagctggccg tgacgacggg
     1261 gaaacccgag agtcgacgcc cgtcgcgctg cggcgagcgg ttccatcagg ccggcggcaa
     1321 gttcgaagag caggttgccg cgcgacctgg cccgcagctc cgcttcctcg ccgccccgca
     1381 tcagtccgag cccgaccgcc accgcggccc gctccagggc gatcggcgtg aaccgatcga
     1441 actcggagtc ggtctgtatc gcgatgaccc tgccccagtt cctccccctg accatgactg
     1501 gtgcggcgag agcaccctcg gcttcagcgt ggtcccgctc cgaccacttc a
//
LOCUS       DAOTIP010000572         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_95890_length_1550_cov_3.954515, whole genome shotgun sequence.
ACCESSION   DAOTIP010000572 DAOTIP010000000
VERSION     DAOTIP010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95890_length_1550_cov_3.954515"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..476
                     /gene="tatA"
                     /locus_tag="P5138_09940"
     CDS             156..476
                     /gene="tatA"
                     /locus_tag="P5138_09940"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRV60939.1"
                     /translation="MLPNIGPLEVVVVLIIALIVFGPKRLPELGKSLGKGIGEFRDGL
                     SNIGHDDDDDDEDEEDHEPAELTPPPTDEEHPLPGEEPVVTEVTGSGEAEQVDGEVVP
                     DSRD"
     gene            485..1255
                     /gene="tatC"
                     /locus_tag="P5138_09945"
     CDS             485..1255
                     /gene="tatC"
                     /locus_tag="P5138_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRV60940.1"
                     /translation="MGKLKPVSHDEQLSLVDHLDELRSRLIYSIIFLSVAFAICFWQS
                     SHILDIAAAPLPEKYQALIVLAPTEAFMTTVTVCAYAAIIVSAPFLSYQIFAYVLPAF
                     SPRERRAILPLLITIPVLFVVGCVFAYFVVLPAAISFLLNFNDDQFAIQLRAREYYSF
                     FSMTLLAGGIVFELPIVILALVRLRILSVEQLRKNRRYAYLICAILAAALPGVDPISM
                     LIETVPLLVLYELSILLARGIGAPKESAESQIPSIQEN"
     gene            1260..>1550
                     /locus_tag="P5138_09950"
     CDS             1260..>1550
                     /locus_tag="P5138_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60941.1"
                     /translation="MADEPEHRMLFDLRGRRKRVIQVIYVLLAILMGASLLLLGGSST
                     LGLQNLFGGDTSVNKSASDANVARANDLQTKFNQQPKNGNLAKELIRARFSAG"
BASE COUNT          280 a          502 c          448 g          320 t
ORIGIN      
        1 gttgacgccg catacggctc gaccgacaag ggctccaagt accagggcca ggcggagccg
       61 accgcctccc ggtattacct gaatttccgg gattagttct ttcgcttccg ggtgatccgg
      121 agccgaaggc cgttcgtagg tacgattgtt agcccatgct tcccaatatc ggccccctag
      181 aagtagttgt tgttctgatc atcgcgctga tcgtgttcgg cccgaagcgt ctgcctgaac
      241 tgggaaagtc gctgggcaag gggatcgggg agtttcgtga cggcctcagc aacatcggtc
      301 acgacgacga tgacgatgat gaagacgagg aagatcacga gccggccgag ctgaccccgc
      361 cgccgaccga tgaggaacat ccgctgcccg gcgaggaacc ggtcgtcacc gaagtgactg
      421 gtagcggtga ggcagagcag gttgacgggg aagtggttcc cgactcccgc gactgagggc
      481 gcccatgggc aaactcaaac ccgtcagcca cgacgagcaa ctcagcctcg tagaccacct
      541 ggatgagctc cgttcgcggc tcatctactc catcatcttc ctctcggttg ccttcgcgat
      601 ctgtttctgg cagagcagtc acatcctcga catcgctgcc gcgccgctgc cggagaagta
      661 ccaggcgctg atcgtcctcg cgccgaccga ggcgttcatg accacggtga cggtatgcgc
      721 ctacgcggcg atcatcgtct ccgccccttt tctcagctac cagatctttg cctacgtgct
      781 gcccgcattc tctccccgcg agcgcagggc gattctgcct ctcctgatca ccattccggt
      841 cctcttcgtg gttggctgcg tcttcgccta cttcgtggtc ctgccggcag cgatcagctt
      901 cctgctcaac ttcaacgatg atcagttcgc gatccagctt cgggcccgcg agtactactc
      961 gttcttctcg atgaccctgc tggccggcgg gatcgtcttc gagttgccga tcgtcatcct
     1021 ggccctggtc cggctgcgga tcctcagcgt cgagcagctg cgcaagaacc gtcgttacgc
     1081 ctacctgatc tgcgcgatcc tcgccgccgc cctgcctggg gtcgacccga tttcgatgct
     1141 gatcgagacg gtgccgctgc tagttctcta cgagttgagt atcttgctcg ctcggggaat
     1201 tggtgcgccc aaggagagcg ccgaatccca gataccttca attcaggaga actagcccag
     1261 tggccgacga acccgaacac agaatgctct tcgaccttcg cggtcgtcgc aagagggtga
     1321 tccaggtcat ttacgtgctc ctcgccatcc tgatgggagc cagcctcctg ttgctcggtg
     1381 gcagcagcac cctgggtctg cagaacctgt tcggtggcga cacctcggtc aacaagtcgg
     1441 cctccgacgc gaacgtggcg cgggccaacg accttcagac caagttcaac cagcagccta
     1501 agaacgggaa cctggcaaag gaactgatcc gggcccgatt ctcggccggc
//
LOCUS       DAOTIP010000573         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_96061_length_1549_cov_1.570950, whole genome shotgun sequence.
ACCESSION   DAOTIP010000573 DAOTIP010000000
VERSION     DAOTIP010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96061_length_1549_cov_1.570950"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..417
                     /locus_tag="P5138_09955"
     CDS             211..417
                     /locus_tag="P5138_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60942.1"
                     /translation="MSDQEILEAIFLLDDDAPAMDIANEIGAPVPDVKARLQELTRTG
                     LVDQTGKAFGLTDVGMDQLEQLTA"
     gene            448..1110
                     /locus_tag="P5138_09960"
     CDS             448..1110
                     /locus_tag="P5138_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60943.1"
                     /translation="MGKEMFASVLWLPEGQEPNWPAARRKAQEIIWDETGEDSELTET
                     GIYSDVEYAVHEARRLGRPHDPNYHLQGLLRDLIGQVEKAVDGENREMLELNRYGWRV
                     FITGGVDDNETELGEALETLNRLDVLAAAGFNPPEQMVETVVYENRHGTSVCVLKDGL
                     GVAQAASIARESWDEITHVRGVPTSPPDDDSEALSIFTEFAGEGGCWINRERLPIQHR
                     VD"
     gene            1249..>1549
                     /locus_tag="P5138_09965"
     CDS             1249..>1549
                     /locus_tag="P5138_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60944.1"
                     /translation="MKNTNNTRTQAQHGLTTRTTSKKTVRRGSRTEIRFGPETETAAD
                     RLTGPGSEARSLMEYHRSKAQEAISNAISRGADRADAIRFARGHLLAGQKAQIQYR"
BASE COUNT          377 a          518 c          462 g          192 t
ORIGIN      
        1 actcgatcga ggtcaccgac gaagaaaccg gcgaaagcct ctacgtcaag gacgccctga
       61 cgccgtcggc gctcaccgac gagtggctca cccagacggt caaacacacg atgtagcagg
      121 gcccgcaagg gccaccgaaa acacccacag accgtccaag cccggacggt cttttcgtgg
      181 ccgaaaacgg ccacagacaa ggagacacac atgagcgacc aggaaatcct cgaggcgatc
      241 tttctgcttg acgacgacgc cccggcaatg gatatcgcca acgagatcgg tgccccggtc
      301 ccggacgtga aagcccgcct tcaggaactc accaggaccg gcttggtgga ccagaccggc
      361 aaggcgttcg gcctgaccga cgtcggcatg gaccagctcg agcagctgac tgcctgaacc
      421 cccgaacaca agaaaggaga agagacgatg ggaaaggaaa tgtttgcctc ggttctctgg
      481 ctgcccgaag gccaggaacc gaactggccg gccgcccgaa ggaaagccca ggaaatcatc
      541 tgggacgaaa ccggcgagga cagtgaactc accgaaaccg ggatctacag cgacgtcgaa
      601 tacgccgtcc acgaagcccg gcgcctcggc cggccccacg acccgaacta tcacctccag
      661 ggactcctgc gagacttgat cggccaggtc gagaaagccg ttgatggcga gaaccgggaa
      721 atgctggaac tcaaccggta cggctggcga gtgttcatca ccggcggcgt cgacgacaac
      781 gaaaccgaac tgggagaggc actcgaaacg ctcaaccggc tcgacgttct cgccgccgcc
      841 ggattcaacc cgcccgagca gatggtcgag acggttgtct acgagaaccg gcacggaacc
      901 tccgtgtgcg tactcaaaga cggtctcggc gtagcccagg ccgcatcgat tgcccgggaa
      961 agctgggatg aaatcaccca tgttcgcggg gttccgacaa gtcctccaga tgacgacagc
     1021 gaagccctgt cgatcttcac cgagttcgcc ggcgaaggcg gctgctggat caaccgtgaa
     1081 cggttgccga tccagcaccg ggtcgactag cagcacctga agcaccgtcg cgggacggcc
     1141 tcccgcacga aaaagcaccc gtcgccagat cctctagggg gggggagaag cagcgacggg
     1201 tgcttcgagc atccctgccc gcgggcagga gcaaggagca acgtagcaat gaagaacacc
     1261 aacaacaccc gcacccaggc ccagcacggc ctgaccaccc gtaccaccag caagaaaact
     1321 gttcggcgcg gatcccgcac cgaaatccgg ttcggacccg aaaccgaaac tgccgccgac
     1381 cggctgaccg gtcccggctc cgaagcccgc tcgctcatgg aataccaccg cagcaaggcg
     1441 caggaagcga tcagcaacgc aatcagccgc ggtgccgacc gggccgacgc gatccggttc
     1501 gcccgcgggc acctgctcgc cggccaaaaa gcccaaatcc agtaccggg
//
LOCUS       DAOTIP010000574         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_96090_length_1548_cov_3.498995, whole genome shotgun sequence.
ACCESSION   DAOTIP010000574 DAOTIP010000000
VERSION     DAOTIP010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96090_length_1548_cov_3.498995"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..340
                     /locus_tag="P5138_09970"
     CDS             44..340
                     /locus_tag="P5138_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRV60945.1"
                     /translation="MPGLIRGVARTAVVAGTATAVSNRVSRRQASRWAEQDGPQYANP
                     RAEMAQQQYQEPAPAPAEAGPDPIAQLKELGELHQSGVLTDEEFAAQKAKILGS"
     gene            365..799
                     /locus_tag="P5138_09975"
     CDS             365..799
                     /locus_tag="P5138_09975"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRV60946.1"
                     /translation="MSDDQIPGVCPPGRASVLARGSGSRGTPRHGLHHEGAHHVSDED
                     FEALIHRAVERLPEEFQKVIAQVPVIISDQGRERHAYGLYQGDGIARDNFPDRIIIFK
                     DTLVRDFGHDPALLAAQVERTVRHELGHHLGYDEGGVHDLGL"
     gene            828..1283
                     /locus_tag="P5138_09980"
     CDS             828..1283
                     /locus_tag="P5138_09980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRV60947.1"
                     /translation="MSKATLNTNAGPITIEFFDDDAPKTVENFRKLAADGFYDGLTFH
                     RVIKDFMIQGGCPLGTGTGGPGYTFEDEFNDHKIVRGALAMANAGPNTNGSQFFIVTI
                     PAADWLDGKHTVFGEITEGMDAVDAIEGTQTDGRDMPLEPQVIESIELA"
BASE COUNT          297 a          537 c          472 g          242 t
ORIGIN      
        1 cgcggccgaa gcctaggaaa acaggaaagg taaggagaag aaaatgccag gactgatcag
       61 aggtgtcgcc cggacggccg tcgtcgccgg caccgcgacc gccgtcagca accgtgtgtc
      121 ccgtcgccag gcctcccgtt gggccgagca ggacgggccg cagtacgcca acccgcgggc
      181 cgagatggcc cagcagcagt accaggagcc cgcccccgct ccggccgagg cgggtccgga
      241 tccgatcgcc caactcaagg aactcgggga gctgcatcag agcggcgtcc tgaccgacga
      301 ggagttcgcc gcgcagaagg cgaagatcct cggcagctga gttcgcggcc aggggtgttt
      361 cgaagtgtcg gatgaccaga tcccgggggt ttgtcccccg ggacgtgcct cggtcctcgc
      421 ccgtggttcc ggttcacgcg gaacgccccg tcacggtctt catcacgaag gcgcccacca
      481 cgtttccgac gaggatttcg aggccctgat ccaccgggcg gtcgaacgcc tgccggagga
      541 gttccagaaa gtcatcgccc aggtccccgt gatcatctcc gaccagggcc gggagcgaca
      601 tgcctacggg ctttaccagg gtgacggcat cgcccgggac aatttccccg accggatcat
      661 catcttcaag gacaccctgg tccgcgactt cggtcacgat ccggctttgc tcgcggccca
      721 ggtggaacgc acggtccggc atgaactcgg ccaccacctc ggctacgacg aaggcggcgt
      781 tcacgacctc gggctttgag ccctcgcccg gcctgtaggc tcgaaccatg tcgaaagcaa
      841 cgctgaacac caatgccggt ccgatcacca tcgagttctt cgatgacgac gccccgaaga
      901 cggtcgagaa cttccgcaag ctcgccgccg acgggttcta cgacggcctc acctttcacc
      961 gcgtgatcaa ggacttcatg atccagggcg gctgcccgct cggcaccgga accggtggcc
     1021 ccggctacac cttcgaggac gagttcaacg accacaagat tgtccgcggc gccctcgcca
     1081 tggccaacgc cggtccgaac accaacggtt cgcagttctt catcgtcacc atccccgccg
     1141 ccgactggct ggacggcaag cacaccgtct tcggcgagat caccgaaggc atggacgccg
     1201 tcgacgcgat cgaaggaacc cagaccgacg gccgcgacat gcccctcgag ccgcaggtaa
     1261 tcgagtccat cgaactcgca tagccgaacc tggtactcca ggtgttcctg ctgcgttaga
     1321 cccggcacgg gtatgcccga aatagaccgt cgccctgcgg gctcccccga aggtcttttt
     1381 cgggcacacc cgcaccgggt ccttctcaat ggtccgggca actgaccccc gggacttgaa
     1441 accgtacccc cgaacccggc ttcaagtctc gaagaccggt tcccgaggta ttgagcaggg
     1501 tccggtggtg tgcttccgaa acctctggat ggggagcgcg aagcgcga
//
LOCUS       DAOTIP010000575         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_96337_length_1545_cov_3.325503, whole genome shotgun sequence.
ACCESSION   DAOTIP010000575 DAOTIP010000000
VERSION     DAOTIP010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96337_length_1545_cov_3.325503"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="P5138_09985"
     CDS             complement(<1..750)
                     /locus_tag="P5138_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HRV60948.1"
                     /translation="MPDLPEKPDAILLDIEGVLYIQGEAIEGAPETLNRLREIAGRIR
                     LVTNTSSVSRNEVIKRVRRAGFDISDEELLTPAAMAVRYCREKGFSKVNLMVARSLRE
                     DLEEIDVVGTDEKADAIVLGDLGPMFSAETLSHAFRQMMDGAELIALQHNRYWRREDG
                     LVLDVGAFSAALEYAAETEAVVVGKPSRDFFRMALADLGVEPGTAVMVGDDIEGDVGG
                     GLGAGIASVLVRTGKYEAEKVAESGIEPTATI"
     gene            complement(879..>1545)
                     /locus_tag="P5138_09990"
     CDS             complement(879..>1545)
                     /locus_tag="P5138_09990"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRV60949.1"
                     /translation="IGQEDGLRLASISLAFNGPVTLGEIEIRAETVKPGRRQRVVSVA
                     IVSGDRTLIEGRAVLIRKGEVELPDGVTVEEEPFPGPESGRPIEKELWADGESDAFHR
                     TSNTVVSIEGGPDRIGTEGMVWIRLDYPLVAGEELTGAQRAVAAADFGNGVAHPVGWG
                     EFLFINCDLNVSLLREPVGEWIGLESRTEVDANGTGLTSTRLHDSRGRFGVASQILFV
                     DRV"
BASE COUNT          248 a          545 c          483 g          269 t
ORIGIN      
        1 gatggtcgcg gtgggctcta tccctgactc ggcgaccttc tccgcctcgt acttgccggt
       61 ccgaaccagg accgaggcga ttcccgctcc cagtccgccg ccgacgtcgc cctcgatgtc
      121 gtcgccgacc atgactgccg ttcccggctc gactccgaga tcggccaggg ccatacggaa
      181 gaagtcgcgg gatggcttgc cgacgaccac cgcctcggtc tccgcggcgt attcgagcgc
      241 cgccgaaaag gcgccgacgt ccagcaccag gccgtcctcc cgcctccagt accggttgtg
      301 ctggagggcg atcagctcgg caccgtccat catctgccgg aaggcgtggc tgagggtctc
      361 ggccgagaac atcgggccga ggtcaccgag cacgatcgcg tcggcctttt cgtcggtacc
      421 gaccacgtcg atctcctcaa ggtcctcccg caacgaccgg gcaaccatca ggttgacctt
      481 cgagaagccc ttttcccggc aatagcggac cgccatcgcg gccggggtga ggagttcctc
      541 gtcggagatg tcgaatccgg cccgcctgac ccgcttgatc acttcattgc gggaaaccga
      601 gctcgtgttg gtgaccaggc ggatccggcc ggcgatctcg cgcagccggt tcagggtttc
      661 cggggccccc tcgattgcct ccccctgtat gtagagcacg ccctcgatat cgagcaggat
      721 cgcgtcgggt ttctcgggca ggtcgggcat aacccaaatg tagaccctgc ggcgcgatcc
      781 aaatctgtcc cggcggataa atggctgcgc aaacggttca ccggacttca gtaagcgaga
      841 ccggggagct gccggtcccg gggatcgccg cccgggaact acacccggtc gacgaacagg
      901 atctggctgg cgaccccgaa acggccccgg ctgtcatgga ggcgggtcga ggtgagcccg
      961 gtgccgttgg cgtccacctc ggtcctcgat tcaaggccga tccactctcc gaccggctcc
     1021 ctgaggagcg aaacgttgag gtcgcagttg atgaagagga attcgcccca gccgaccggg
     1081 tgggcgactc cgttgccaaa atccgctgcc gcgacggccc gttgggcgcc ggtcagctcc
     1141 tcgcccgcta ccaggggata gtcgagccgg atccagacca tgccctcggt gccgatccgg
     1201 tccgggccgc cctcgatcga caccacggtg ttgctcgtgc ggtggaaggc gtcactttcc
     1261 ccgtccgccc agagttcctt ctcgatcggc cgcccgctct ccggcccggg gaacggttct
     1321 tcttccaccg tcacgccatc cggcaactcg acttccccct tgcggatcag caccgcccgg
     1381 ccttcgatca gggtccggtc accggagacg atcgctaccg agacgacccg ctggcgccga
     1441 ccgggcttga ccgtctcggc cctgatctcg atctcgccaa gagtcaccgg cccgttgaag
     1501 gcgagggaga tggaggcaag acggagtccg tcttcctggc cgatc
//
LOCUS       DAOTIP010000576         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_96441_length_1544_cov_3.139691, whole genome shotgun sequence.
ACCESSION   DAOTIP010000576 DAOTIP010000000
VERSION     DAOTIP010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96441_length_1544_cov_3.139691"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="P5138_09995"
     CDS             complement(<1..904)
                     /locus_tag="P5138_09995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV60950.1"
                     /translation="MYSTGGIADRFVPLWSRVEGRLGQPLPAALRFWDGSEIGPGEEG
                     SCPDIIVLRNKRALSYAASRPDQLGIARAFVSGDLILEGSIERVMAAGARLYGFDFTI
                     HDKLEAVKLAAQIGAIKIPPPKPPESEAKPKGRLHSLRRDSDSIQHHYDVSNEFYRQV
                     LGPTMVYSCAYFNSPDDSLEEAQTRKLDVVCQKLRLESGERLLDIGCGWGSMAIHAAA
                     NYGVEVVGVTLSPSQAERARLRALREDVERDLAAAGPAPQPPSYDDMRTECLGQQSAQ
                     LLAYAKTVKRADGTTMHEAFEKWAR"
     gene            complement(1027..1230)
                     /locus_tag="P5138_10000"
     CDS             complement(1027..1230)
                     /locus_tag="P5138_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRV60951.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHTAIQADGFRTLAE
                     GAKVSYESEEGPKGPAAASVEAI"
     gene            complement(1329..>1544)
                     /locus_tag="P5138_10005"
     CDS             complement(1329..>1544)
                     /locus_tag="P5138_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRV60952.1"
                     /translation="NPNTMYPLLRDLESKGLIEGSWEHPDKRTRRFYSITAAGEEAYR
                     ELLPEIEPFLDSVIRSVTMIKAEVYGS"
BASE COUNT          255 a          510 c          479 g          300 t
ORIGIN      
        1 cacgcgccca cttctcgaac gcctcgtgca tcgtggttcc gtcagcgcgc ttcaccgtct
       61 tcgcgtaggc caacagctgc gccgactgct gaccgaggca ttcggttcgc atgtcgtcgt
      121 agctcggcgg ctgcggcgcg ggcccggcgg ctgcgaggtc tcgctcgaca tcctcgcgca
      181 gcgcgcgcag acgggccctc tcggcctgtg acggcgaaag agtcacgccg accacttcga
      241 ccccgtagtt ggccgccgcg tgaatagcca tcgaccccca gccgcagccg atgtcgagca
      301 gccgctcccc ggactccagc cgcagcttct ggcagaccac gtccagcttg cgggtctggg
      361 cctcctcgag gctgtcatcg ggcgagttga agtaggcgca ggaatagacc attgtcggtc
      421 cgaggacctg gcgatagaac tcgttcgaga cgtcgtagtg gtgctggatc gaatcgctgt
      481 cgcgccgcag cgaatgaagg cggccctttg gctttgcctc ggactcgggc ggcttcggcg
      541 gggggatctt gatcgccccg atctgggcgg ccagcttcac cgcctcgagc ttgtcgtgga
      601 tcgtgaagtc gaaaccgtag agcctggccc cggccgccat cactctttcg atgctgcctt
      661 cgaggatcag gtctccggag acgaaggcac gtgcgatccc gagctgatcg ggacggctgg
      721 cggcgtaact cagggcacgt ttgttccgca gcacgatgat gtccgggcag gaaccctctt
      781 cgcccggacc tatttcactt ccgtcccaga aacgcagggc ggccgggagc ggctggccga
      841 gcctcccttc taccctggac cagaggggaa cgaaccgatc ggcaatacca cctgtgctgt
      901 acatgccccc gcaagttact cctgcttcgg tttcgcctca accctccggt tcacttcgac
      961 gttccggacc tgaaacgaaa acggccgccc ggaggcggcc gtttcaaact gcttccgaga
     1021 ccgggttcag atggcctcga cggaagcggc tgcggggccc ttcgggccct cttcggactc
     1081 gtagctcacc tttgcacctt cggccagggt gcggaacccg tcggcctgga ttgcggtgtg
     1141 gtggacgaag acgtccttgg agccatcatc aggggtgatg aatccgaagc ccttctcgtc
     1201 actgaaccac ttgactgtcc cagtcggcat aacttcctcc aacatcatga tgcggtgccg
     1261 gttctctcct cccggcctga cgcgggcaaa ctaccactag cagcgtgatc cgggggatcc
     1321 ggggccgttc aggacccgta aacctcggcc ttgatcatgg tcaccgagcg aatcaccgag
     1381 tcgaggaacg gctcgatctc gggcagcagt tcgcggtacg cctcttctcc ggcagcagtt
     1441 atcgagtaga agcggcgggt ccgcttgtcc ggatgctccc agctgccttc gatcagaccc
     1501 ttgctctcga gatcgcgcag caacgggtac atggtgttgg ggtt
//
LOCUS       DAOTIP010000577         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_96710_length_1541_cov_2.666891, whole genome shotgun sequence.
ACCESSION   DAOTIP010000577 DAOTIP010000000
VERSION     DAOTIP010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96710_length_1541_cov_2.666891"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="P5138_10010"
     CDS             <1..334
                     /locus_tag="P5138_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRV60953.1"
                     /translation="RNGSSPVIPESIITRPPSAELRPDQLDTDSLPDYDQLDRILRMY
                     VEEDLGPAEIAGLGEDPEVVADVVAKVDRAEYKRRQSPPGLKITTKGFGRDRRMPITN
                     RYRPWRAD"
     gene            complement(331..759)
                     /locus_tag="P5138_10015"
     CDS             complement(331..759)
                     /locus_tag="P5138_10015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRV60954.1"
                     /translation="MPDVTLPESPFDNLLGTEWISSDPDQAIVRLTMRDDHRQPMAIM
                     HGGVMGSLVESVCSMATAHAVLPEGRIAMGQSISVNLLRPISSGTVEVVATAVHRGRM
                     TWVWRAEVKDEEERICAVAQMTMAVREAPEEADLSQFVKP"
     gene            871..>1541
                     /locus_tag="P5138_10020"
     CDS             871..>1541
                     /locus_tag="P5138_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60955.1"
                     /translation="MSEDSRALLQLLLGRGKSYADISGLLGIDEAEVRSRASTALSEL
                     DDSTAAPDTEMTDYLLGQADPISRADVGNRISSDPAAAEQAEAITDQLRLLIPGADLP
                     KPGSAQGGKPKPPDRPRPAPAASSEMAPSGASSSGGFAAITSHQRRLIAVLLGGALLA
                     AVVILLATGVIGGSDDGDSATPKSSPTVAVPAGDCRNGWFRKPGFDAATDADVAGAVL
                     LGERQL"
BASE COUNT          252 a          535 c          503 g          251 t
ORIGIN      
        1 ccggaacggt tcctcgccgg tgattccgga gtcgatcatc acccggccac cgtcggcgga
       61 gttgcgtccg gaccagctgg acacggactc cctgcccgac tacgaccagc tggaccgcat
      121 cctgaggatg tacgtcgagg aagacctggg accggccgag atcgcgggcc tcggcgaaga
      181 ccccgaggtg gtggcggacg tggttgcgaa ggtggaccgg gccgaataca agcgtcggca
      241 gtcacctccc ggtctgaaga taaccaccaa gggattcggc cgggaccgcc ggatgcccat
      301 tacgaaccgt taccggccct ggcgggccga ttagggcttc acgaactggc tgagatcggc
      361 ttcctcgggg gcttccctta ctgccatggt catctgggct acggcacaga tccgttcttc
      421 ttcgtccttg acttcggccc gccataccca ggtcatccgt ccacggtgta cggctgtcgc
      481 gacgacttcc accgttccgg atgagatcgg acgcagcagg ttcaccgaaa tgctctgtcc
      541 catcgcgatc cggccctccg gcagaaccgc gtgggcagtt gccatcgagc agacgctctc
      601 gaccaggctc cccatgacgc caccgtgcat gatcgccatc ggctggcgat ggtcatcccg
      661 catggtcagc cggacgatgg cctgatcggg gtcggaggaa atccactcgg tgccgaggag
      721 attgtcgaac ggggattcgg gaagcgtgac gtctggcatc agccgggatc atatgcgccc
      781 agtggcggcc gccggtggcc ggttccggac tcagggatca ctcaggaaac agccgggggc
      841 cgcgaaggcg tagggttcac gctcatgccg ttgagtgagg acagtcgggc gctgcttcag
      901 ctgctgctcg gccgagggaa gagttacgcc gacatttccg ggctgctcgg aatcgatgag
      961 gccgaggtgc gcagccgcgc ctccaccgcc ctctccgagc tcgatgactc tactgccgcg
     1021 ccggacaccg aaatgaccga ctacctgctc ggccaggccg accccatctc cagggccgac
     1081 gttggcaacc ggatttcctc cgacccggct gccgcggagc aggcggaggc gatcaccgac
     1141 cagcttcgcc tcctgatccc gggtgccgac ctgccgaaac ccggctcagc ccagggcggc
     1201 aagccaaagc ccccggatcg gccccggccg gccccagccg ccagcagcga aatggcaccg
     1261 tccggggcca gctcttccgg cggctttgcc gcgatcacca gtcatcagcg gcggctcatc
     1321 gcggtactgc tcggcggcgc tctgcttgcc gctgtggtga tcctgctggc caccggtgta
     1381 atcggcggct cggatgacgg cgattcagcc acgcccaagt cgtcgccgac cgtggccgtc
     1441 cccgccggcg actgccgcaa cggctggttc cgcaagcccg gcttcgacgc cgcgaccgat
     1501 gccgacgtcg cgggcgccgt gctgctcggc gagcgccagc t
//
LOCUS       DAOTIP010000578         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97336_length_1534_cov_2.701149, whole genome shotgun sequence.
ACCESSION   DAOTIP010000578 DAOTIP010000000
VERSION     DAOTIP010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97336_length_1534_cov_2.701149"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="P5138_10025"
     CDS             <1..411
                     /locus_tag="P5138_10025"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60956.1"
                     /translation="GLYDTRMLSWMFWAYNENLVTEESTPNPGSVVNQPVLQALTRPY
                     PVALTGKPTSLSFDPATRVMSLKFKTARTGGGKFIRRLPSVIATQKLVYPDGYRVRVT
                     GATVTSRPCAGRLTLRNHARAKAVSVTVKPGGTC"
     gene            663..>1534
                     /locus_tag="P5138_10030"
     CDS             663..>1534
                     /locus_tag="P5138_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRV60957.1"
                     /translation="MAAILAGVWSVATGPASADDPYPDPGFPVIPCSSADDTVVLTAD
                     AQLDPSCTYTKGVEITASDVTLDCRGASVATEKTKGVGILIETPADQNLDQVTVRNCE
                     VSGFLNGMKITRDGFRDLADGGEYDNWLKDVDLENNTVTGTKGVGIFVDGYVTYTTIR
                     DSVIEGAGSSGIYLEAGSADNTVSDNRIIDNGFGENGPDGQVESFNGVQLRVWGTGRE
                     GLSIDGSRRNTVSGNTFEGNSAGGIYLYTNCGEYRNSKPDRYFERRFGATGNLIDGNH
                     LIGGVSGVWVASRMG"
BASE COUNT          297 a          490 c          494 g          253 t
ORIGIN      
        1 ggcctatacg acaccaggat gctttcctgg atgttctggg cctacaacga gaacctggtc
       61 accgaggagt ccactccgaa ccccgggtcg gtcgtgaacc agccggtgct tcaggcgctc
      121 acgcggccat accccgtagc cctgaccggc aagccgacca gcctttcctt tgaccctgcc
      181 acgcgagtca tgtcgctgaa gttcaaaacc gcccgaaccg gtggcggcaa gttcatccgc
      241 aggctcccca gcgtgatcgc cacccagaag ctggtctatc ccgacggcta ccgggtcaga
      301 gtcaccggcg ccacggtcac ttcgcgtccc tgtgccggac ggctcacgct gcgaaaccac
      361 gccagggcga aggcggtcag cgtgaccgtc aagcccggcg ggacctgctg acgccgtacc
      421 gctctgcgat cagcatgtcc ggcgatggcc gacgtgggtc gccagcgggg aatgaagaac
      481 ctggaacggc cgccagggca cggcacaccg atcgcgatct ctcccgctgg accacatcag
      541 cggcaccatc gggcttctgc ccctttcttg ttgaagttgg gcaggtcaat ggatacggtt
      601 cgtgccggtt cattcagcag gacggaaagg gagatttgcg actcggtaag gcagggctgt
      661 tggtggcggc gattctcgcc ggggtctggt cggtggccac tggccccgcg agtgcggatg
      721 acccctatcc cgacccgggc ttcccggtta ttccctgctc gagcgccgat gacacagtgg
      781 tgctcaccgc ggatgcccag ctggacccct cctgcaccta caccaaggga gtcgagatca
      841 cggcatccga cgtgacgctc gactgccgcg gcgcatcggt cgccaccgaa aagaccaagg
      901 gcgtcgggat cctcatcgaa acgccagccg accagaacct ggaccaggtc accgttcgca
      961 actgcgaggt cagcggtttc ctcaacggca tgaagatcac gcgggacggg ttccgggacc
     1021 tggcggacgg cggggaatac gacaactggc tcaaggatgt tgacctcgaa aacaacacgg
     1081 ttaccgggac gaagggggtc gggatattcg tcgacggcta tgtgacctac acgacgatcc
     1141 gcgactcggt gatcgagggt gccggcagca gcggcatcta ccttgaggcg ggctcggccg
     1201 acaacaccgt cagcgacaac cggatcattg acaacggttt cggcgagaac ggcccggacg
     1261 ggcaggtgga gagcttcaac ggtgtccagt tacgcgtctg gggcaccggc cgcgagggcc
     1321 tttcgatcga cggttcccgg cgcaatacgg tgagcggcaa cactttcgaa ggcaactcgg
     1381 ctggcggaat ctacctctac accaactgcg gcgagtacag gaactcgaag cccgaccggt
     1441 acttcgagcg ccgtttcggc gcgaccggaa acctgatcga cggaaatcac ctgatcggcg
     1501 gggtgagcgg ggtttgggtg gcctcccgga tggg
//
LOCUS       DAOTIP010000579         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97393_length_1533_cov_3.935724, whole genome shotgun sequence.
ACCESSION   DAOTIP010000579 DAOTIP010000000
VERSION     DAOTIP010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97393_length_1533_cov_3.935724"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..483
                     /locus_tag="P5138_10035"
     CDS             187..483
                     /locus_tag="P5138_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60958.1"
                     /translation="MTGVADDQKFCLEFADFTGELPWLLEKAEAIEDAMGEQLAGAFL
                     WHSVVAEPDIDGFRVFFEVDPVGPGLDRSVPEKLIGIIREIVMSDPPGDQELAA"
     gene            517..963
                     /locus_tag="P5138_10040"
     CDS             517..963
                     /locus_tag="P5138_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60959.1"
                     /translation="MDDEILTRTFCIEVTHFPGVDEAWLEERTLAIGHAIKDRLIHKL
                     EWGRVYALPDRNGIGLVFLMWPEYKHDETVTETFMEIICEKTDIVMPKPGSRKKPYRK
                     GGMPQFGLGPRFPAEFFASHGKIDPSMGPLQSSHGKDRYSRTQRSA"
     gene            923..1156
                     /locus_tag="P5138_10045"
     CDS             923..1156
                     /locus_tag="P5138_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRV60960.1"
                     /translation="MAKIDIQELSDQPEEAIDRVRSGEEIIITRDGEDVAELGPVRKG
                     LSAEEVVERHRNLSPMDPESLRRDIDEIFAPGL"
     gene            1220..>1533
                     /locus_tag="P5138_10050"
     CDS             1220..>1533
                     /locus_tag="P5138_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60961.1"
                     /translation="MIDGLTPDEFVANSGWRYARTMPDIPHQYTVRDLESPDGRRTTA
                     MGSDEFEWFVRLIRAEGVMKTWGRRTHPYLQVGGWEYWTMGDPAEETTIINRQPVTPE
                     AMA"
BASE COUNT          303 a          429 c          496 g          305 t
ORIGIN      
        1 atgatgatca agagctcgac ttcagcatgg gtgggcagct acctcccgac catcctgacg
       61 ccgacctgct tgatgatctt gatctctgac ttctgagcag ccgggggcga gtttctcttg
      121 cccgggactg actctcttgg gtggccgttg ttgcggccac ggaatcgtcc gaaagagagg
      181 gtcgtgatga cgggtgttgc tgatgatcag aagttctgcc ttgagttcgc tgacttcacc
      241 ggggagttgc cctggttgct ggagaaggcg gaggcgatcg aggatgcgat gggcgagcag
      301 ctggccgggg cctttctctg gcactccgtc gttgccgaac cggacatcga cgggttccgg
      361 gtcttcttcg aggtcgaccc cgtcgggccg ggtctggacc gttcggtgcc cgagaagctg
      421 atcgggatca tccgggagat cgtcatgagc gacccgcccg gcgaccagga gctggcggcc
      481 tgagcccact gctttccgga cctaggagac ggagctatgg acgacgagat cctgacccgc
      541 accttctgca ttgaggtcac ccactttccc ggcgtcgatg aggcctggct ggaggagcgg
      601 acccttgcga tcggccatgc gatcaaagac cggctcattc acaagctcga atggggtcgg
      661 gtctatgccc tgccggaccg caacggaatt gggctcgttt tcctgatgtg gccggagtac
      721 aagcacgacg agacagtcac cgaaaccttc atggagatca tctgcgagaa gaccgacatc
      781 gtcatgccga aaccgggcag caggaagaag ccctaccgca aaggcggaat gccgcagttc
      841 ggcctgggcc cgagattccc ggccgaattc ttcgcgagcc acggcaaaat tgacccgagt
      901 atgggccctc tacaatcgag ccatggcaaa gatcgatatt caagaactca gcgatcagcc
      961 tgaagaggca atcgaccgcg ttcgaagcgg cgaggagatc atcatcactc gcgatggtga
     1021 ggatgtggcc gaactggggc cagtgcgaaa gggactttcc gccgaagaag tcgtcgagcg
     1081 acaccggaac ctttccccga tggatcctga aagtctgcgt cgggatatcg atgagatctt
     1141 cgccccgggt ctctgagccc gctacttcgg tattggctga gtcccgtcag attttcggat
     1201 ctgccgagac tttctgggca tgatcgatgg gttgacccct gacgaattcg tagccaactc
     1261 cggctggcgc tatgcgagga cgatgcctga tattcctcat cagtacacgg tgcgggatct
     1321 ggaatctcct gatggacgac ggaccacggc gatgggttcg gatgagttcg agtggttcgt
     1381 gcggttgatc cgggccgaag gggtgatgaa gacctggggc cgacggaccc atccctacct
     1441 tcaggtcggc ggctgggagt actggaccat gggtgatccg gctgaggaga cgactatcat
     1501 caaccggcag ccggtcaccc cggaggcgat ggc
//
LOCUS       DAOTIP010000580         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97507_length_1532_cov_2.834800, whole genome shotgun sequence.
ACCESSION   DAOTIP010000580 DAOTIP010000000
VERSION     DAOTIP010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97507_length_1532_cov_2.834800"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5138_10055"
     CDS             <1..659
                     /locus_tag="P5138_10055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRV60962.1"
                     /translation="APMNQRGKLVTLGVIGIALAVVVVVVLVGRGGDDDGGETATPVT
                     KDVKLEKPQPGSVVKQGEVAHVKMETTEGDFTIDLDTTDWPITANSFAYLTEKKFYDG
                     LGFHRIVPDFVIQGGDPTGSGSGGPGYSVVETPPQDTKYPIGTVAMAKTGSEAPGTSG
                     SQFFVVTGAQGSSLTPDYAIAGKVTDGMDVVEAIGNLGSADETPTKQVVIKKATLEQG
                     "
     gene            726..1499
                     /locus_tag="P5138_10060"
     CDS             726..1499
                     /locus_tag="P5138_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV60963.1"
                     /translation="MDGRGSGILLCHGLSATRRYVVHGSRHLPRRGYRLITYDARGHG
                     QSDPAASYEYAGLADDLDLVIDATGAMESEGAVLGGHSMGCHTVTRRALANPDGVRAL
                     ILIGPVFTGQTDDDSRWDRRADALENGGPEAFAEVVAENSPAGKARETSFRLARDRAR
                     LHLHPEAVAQALREVPRSRPFGSLEELADLGMPVLVVASHDEFDPGHPREVAERWTEA
                     IPGAELIGEEKGQSPLAWQGGMLSREIDSFLERHSLGPA"
BASE COUNT          275 a          464 c          549 g          244 t
ORIGIN      
        1 tggcgccgat gaatcagcgc ggaaagcttg tgactctggg agtgatcggc atcgccctgg
       61 ccgtcgtggt cgtggtggtc ctggttggac ggggcggtga cgacgacggt ggcgagactg
      121 ctaccccggt caccaaagac gtgaagctgg aaaagcctca gcccgggtcg gtggtgaagc
      181 agggtgaggt ggcccacgtg aagatggaaa caacggaggg cgacttcacg atcgacctgg
      241 acacgaccga ctggccgatc accgcgaaca gcttcgccta cctgaccgag aagaagttct
      301 acgacggtct cggtttccac aggatcgtgc ccgacttcgt gatccagggc ggggatccga
      361 ccggatcggg ttcgggaggt cccggctaca gcgtggtgga gacgcctccg caggacacca
      421 agtacccgat cggcaccgtc gcgatggcga agaccgggtc ggaagcaccc ggtacctccg
      481 gcagccagtt cttcgtggtc accggggccc agggctcaag cctgaccccc gattacgcga
      541 tcgccggcaa ggtgaccgac ggaatggacg tggtcgaggc gatcggcaac cttggctctg
      601 ccgacgagac accgaccaag caggtcgtca tcaagaaggc gacactcgag cagggctgaa
      661 cgctgtcggc cgctgaaccg gagcagttca cggccgatgc cggcggccgg aagctggctg
      721 gcgagattga cggtcggggt tccggaattc tgctctgcca tggtctttcg gcgacccgac
      781 gctacgtggt ccacggctcc cggcaccttc cgcggcgcgg ctaccggctg atcacctacg
      841 acgcccgcgg ccacggtcag agcgatccgg cggctagcta cgaatacgcg ggtctggcgg
      901 atgacctcga cctggtgatc gatgcgaccg gagcgatgga gtccgaaggg gcagtgctgg
      961 gcggccattc gatgggttgc cacacggtga cccgacgggc gctggctaat cccgacggag
     1021 tccgggccct gatcctgatc ggacccgtgt tcaccgggca gaccgatgac gactcgcgtt
     1081 gggacaggcg ggcggatgcg ctcgagaatg gcggtccgga agcctttgca gaggttgtgg
     1141 cggagaacag cccggccggg aaggcgagag aaacttcctt ccgcctggcc cgcgaccggg
     1201 cccgcctcca tcttcacccg gaggcggttg cccaggccct gcgcgaagtg ccacgatcgc
     1261 gtccgttcgg gagcctggaa gagctggcgg atctggggat gccggttctg gtcgtagcca
     1321 gccacgacga atttgacccg ggacatccgc gggaggtggc tgaacgctgg accgaggcga
     1381 tacccggcgc ggaactgatc ggcgaggaga agggccagtc accgctcgcc tggcaggggg
     1441 ggatgctgtc gcgtgagatc gactccttcc tggagcggca ttccctcggg ccggcgtgaa
     1501 gcgacgggta gccttccctt catggatgtg at
//
LOCUS       DAOTIP010000581         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97527_length_1532_cov_2.314827, whole genome shotgun sequence.
ACCESSION   DAOTIP010000581 DAOTIP010000000
VERSION     DAOTIP010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97527_length_1532_cov_2.314827"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1532
                     /locus_tag="P5138_10065"
     CDS             <1..>1532
                     /locus_tag="P5138_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60964.1"
                     /translation="TVTNSQDLSWAISLAADMLHHWPMTDSSVSGEPLPYHNFGIIHV
                     ASISQVSGVELPVAFFRLWSLPLVVLIALQFVWTGRALFRSVGVGLPALLVFFFISET
                     HLVVDPNNFSLFGWLSPILIFTSPSYLFGLPFLLTLFVLVGETLVADDRRTPGYRDIL
                     VFALVAVGAAGAKVSIIPLLLPALGIYLVYRFLRERRIDRAAVVLAAVFAAVQLVFYW
                     TLYRGHSSGLYLDSGAIDLMSNWQGALAIRQELDSFLPGVLARALAIGLDFALMLVAP
                     LAGLYFVFRDPQQRKSRLTTWFMAILLIALVSLVFVSSHVSGNQLYGVNYGFVGAMIL
                     ASSGLVLGWRARTDGWERQGAVRWLALGWLVAVSILVVIQLLFEVSSTLFGIALPIGA
                     FLTFAFIANRILRRSGIGGTALLLSGSLLLSGFLGAQFNYRWNAIVDPPAPVLTDYRM
                     TPYIYEGLRWLRAETPEDSVFAVNDQYAYSWNFWPVAYDYSAFSERRAFFAAWAYSQR
                     VR"
BASE COUNT          190 a          535 c          455 g          352 t
ORIGIN      
        1 agacggtcac caactcacag gatctctcct gggcgatttc actcgcggcg gacatgcttc
       61 accactggcc gatgaccgac tcgagcgtct ccggtgagcc gctgccctac cacaacttcg
      121 ggatcatcca tgttgcttcc atcagccagg tcagcggggt cgagctcccg gtggccttct
      181 ttcggctgtg gagcctgccc ctggtggttc tcatcgccct tcagtttgta tggaccggcc
      241 gggccctatt tcgcagcgtc ggcgtagggc tgcccgcgct gctggtcttc ttcttcatca
      301 gcgagaccca cctggtggtc gacccgaaca acttctccct cttcggctgg ctctctccga
      361 tcctgatctt caccagcccg agttaccttt tcgggcttcc cttcctgctc accctgttcg
      421 tgctcgttgg cgagaccctg gtcgcggatg accgccggac ccccgggtac cgggacatcc
      481 tggtcttcgc cctggtggcg gtcggtgcgg ccggggccaa ggtctcgatc atcccgctgc
      541 tcttgcccgc gctggggatc tatctggtct accgcttcct gcgggaacgc cgaatcgacc
      601 gggcggcggt ggttctggcg gcggtcttcg cggcggttca gttggtcttc tactggacgc
      661 tttaccgcgg ccacagcagc ggtctgtacc tcgactccgg cgcgatcgac ctgatgtcca
      721 actggcaggg agccctggcg atccggcagg agctggattc cttccttccc ggagtgctgg
      781 ccagggccct ggccatcggc ctcgacttcg ccctgatgct cgtcgctccc ctggccgggc
      841 tctactttgt ctttcgggac ccgcagcaac gtaagtcgag gctgaccaca tggttcatgg
      901 cgattctcct gatcgccctg gtcagcctcg tattcgtcag ctcccacgtc tcgggcaatc
      961 agctctacgg cgtcaactac ggattcgtcg gggccatgat cctcgcctcc tccggtctgg
     1021 ttctcggctg gcgggctcgt accgacggct gggagcggca aggtgccgtg aggtggctcg
     1081 ccctgggctg gctggtcgcg gtgtccatcc tggtcgtgat ccagctcctt ttcgaggtgt
     1141 cctcgaccct cttcggcatc gccttgccga tcggcgcctt cctcaccttc gctttcatcg
     1201 ccaaccggat cctgcggcgc tccggcatcg gcgggacggc gctgctgctc tccggatcgc
     1261 tgctgctctc cggctttctc ggagcgcaat tcaactaccg ctggaacgcg atcgtcgatc
     1321 cgcctgcgcc ggtgcttacc gactaccgga tgacccccta catctacgag ggcctgcgct
     1381 ggctgcgggc cgagactcca gaggactcgg tattcgcggt caacgaccag tacgcgtatt
     1441 cctggaactt ctggcccgtc gcctacgact actcggcctt ctcggagcgt cgcgccttct
     1501 ttgccgcctg ggcctactcg cagcgggtga gg
//
LOCUS       DAOTIP010000582         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97563_length_1531_cov_4.386856, whole genome shotgun sequence.
ACCESSION   DAOTIP010000582 DAOTIP010000000
VERSION     DAOTIP010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97563_length_1531_cov_4.386856"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>1531)
                     /locus_tag="P5138_10070"
     CDS             complement(179..>1531)
                     /locus_tag="P5138_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRV60965.1"
                     /translation="TVLTDTIALVILAVVAGTQSGTGSTSDVILELGLGFLSLGLVCF
                     VALPFLARRAFRFLGQERTVRYAIAIASFLIAAVVAETFGIEGIVGAFFAGLAMNRLV
                     PTEGPLMNRIDFFGGAVFIPVFLVSVGLLLKPAVMFEGETIGLAALLIVACLGGKYIA
                     GQLTRLILKATGPQANLAFALSAPQAAATLAATTVGFNIGLFGESVVNAVLVLILVSV
                     LVATIVAERAKSRIELPEELTPELGERVLVAIANLEGAQLGLRLARSVARREAGKVEV
                     LLLAGEDSPPRQRRAELDRLSGTCRRLGIDADPLIRITDNTARTTLFAAHDFNASLVL
                     AVTDPGQEEWVRSVGLKVSCPIAVVLGDVDAPLGEVVKGEGPDASGLVTDFGDAVESN
                     HRSSDHATVRIRVLDGWSDLPGQAPPEGTGMVLLHPGPASVDPDEAWQEPTRMGSALT
                     "
BASE COUNT          278 a          530 c          493 g          230 t
ORIGIN      
        1 gcggtgcgcc gtttccggat cagaggggac cggacgggtc acaacgctcg ctgaggtttc
       61 gccgcatcgg tgaagaccca ccgcatcgct gaagacccac cgcatcgctg aagacccgcc
      121 gggtcgataa agaccgatcg catcgccaaa gacccgccga tgccggccaa ttccggtctc
      181 aggtcagggc gctgcccatc cgcgtgggct cctgccaggc ctcgtccgga tcgacggacg
      241 ccggccccgg gtggagcagc accatcccgg ttccctcggg cggggcctgc ccggggaggt
      301 ccgaccagcc gtcaagaacc ctgatcctga ccgtcgcgtg gtcgctcgag cgatggttgc
      361 tttcgaccgc atcaccgaaa tcggtgacca gtccactcgc gtcgggacct tctcccttca
      421 cgacctcacc gagcggcgcg tccacatccc caagcaccac ggcgatcggg cagctgacct
      481 tcaggccgac cgacctgacc cattcttcct gtccggggtc ggtcacggcg aggaccagcg
      541 acgcgttgaa atcgtgagcg gcaaaaaggg tagtccgggc ggtgttgtcg gtgatcctga
      601 tcagcggatc cgcgtcgatc ccgagccggc gacaggttcc gctcaaccgg tcgagttcgg
      661 cccgacgctg ccggggcggt gagtcttctc cggccagcag caggacctca acctttcccg
      721 cctcccggcg ggcgacgctc cgggcgagcc ggagtccgag ctgggcgccc tcaaggtttg
      781 cgatcgccac cagaaccctt tcgcccagtt ccggggtcaa ctcttccggc agctcgatcc
      841 tggacttcgc acgctcggcg acgattgtcg cgaccagcac actgaccaga atcaggacca
      901 gcaccgcgtt cacaacggac tctccgaaga gcccgatgtt gaagcccaca gtggtcgcgg
      961 cgagtgtggc ggctgcctgc ggggccgaga gcgcgaacgc caggttcgcc tgggggccgg
     1021 tcgccttgag tatgagccgg gtcagctgtc ccgctatgta cttgccgccg aggcaggcga
     1081 cgatcaagag cgccgccagg ccgatcgtct cgccttcgaa catcacggcc ggcttgagca
     1141 gcagcccgac cgacaccagg aagaccggga tgaagaccgc gcccccgaag aagtcgatcc
     1201 ggttcatcag gggtccctcg gttggcacga gccggttcat ggccagcccg gcgaagaagg
     1261 ccccgacgat gccctcgatc ccgaaagtct cggccacgac cgcggcgatc aggaaggaag
     1321 cgatcgcgat cgcgtacctg accgtccgtt cctggccgag gaaccggaag gcacgacgtg
     1381 cgagaaacgg cagggcgacg aaacagacca gacccaggct gaggaagccg agaccgagct
     1441 ccaggatcac gtcgctggtg ctgcccgttc ccgattgcgt tcccgcaacg accgcgagga
     1501 tcaccagcgc gatcgtgtcg gtcaggacgg t
//
LOCUS       DAOTIP010000583         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97657_length_1530_cov_3.993220, whole genome shotgun sequence.
ACCESSION   DAOTIP010000583 DAOTIP010000000
VERSION     DAOTIP010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97657_length_1530_cov_3.993220"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1530)
                     /locus_tag="P5138_10075"
     CDS             complement(<1..>1530)
                     /locus_tag="P5138_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60966.1"
                     /translation="SPVGAVDIFVQIRAASPVTGSINPVTGEVSLSTDLTIQLTSNHA
                     IVALGNNCYVGTPASPIPFSASSDTTGGVPYDEYPATATVVDDTLAIPAASGCPTIVG
                     QDVNQIVNSTMGLPSPSGENKATLAMRFNPAPHSENWVDPNGPDDIPPTIVNDTNVEV
                     DTGVLQRSWLESGTISDNTVDDGLGTVTGAGRDGNTIRITLLVKHNPGRQVTGVRVDD
                     NWDASDDADGSAIKPVTEEQPAVVGGFNYSRVTFAYQAPSAGMGLTCPGLFDSNQGVQ
                     ARTAQISVRAVLDNGDESDTSVSAIKLTREDCNNKFDPPLLYGWQDQDPAGPITPGTE
                     VAFRFRGIDTDEGLTSQQKFGGYTWRLRNLDTGETTTPTQVCPGTNLDGVLRTLNVTF
                     PKRGRWVVEAQLLDMANDLFDNQGCVDPLSGNFWSWIGAVDVNSPAEIANVRSPGASL
                     ALPARPGTDNTITASVETSDPFDVPNDAGGLTQSVEWDIDGDNSNGVDGFDLARLGDS
                     RT"
BASE COUNT          234 a          470 c          530 g          296 t
ORIGIN      
        1 cgtccggctg tcacctagcc gggccagatc gaatccgtcc acaccgttgc tgttgtctcc
       61 gtcgatgtcc cactcgaccg actgggtcag cccgccggcg tcgttcggca cgtcgaaggg
      121 gtcgctggtc tcgaccgagg ctgtgatcgt gttgtcggtc cccggtcgcg cgggcagggc
      181 caggctcgcg cccgggctcc tgacgttggc gatctcggcc ggtgagttca cgtcaacggc
      241 accgatccat gaccagaaat tgccggaaag cggatccacg cagccctggt tgtcgaagag
      301 gtcgttggcc atgtcgagca gttgtgcttc aaccacccag cgtccgcgct tcgggaaggt
      361 gacgttcagg gtccggagca ctccgtccag attggtgccg gggcagactt gggtcggcgt
      421 cgttgtttca ccggtatcga ggttgcgaag ccgccaggtg tagccgccga acttctgctg
      481 agaggtcaaa ccctcatcgg tgtcgattcc gcggaacctg aaggccacct cggtgcccgg
      541 ggtgatcggc ccggccggat cctgatcctg ccatccgtag agcaggggcg ggtcgaactt
      601 gttgttgcag tcctcccggg tcagcttgat cgccgaaacc gaggtgtcgg attcgtcacc
      661 gttgtccagc accgcccgga cgctgatctg ggcggttctg gcctgtactc cctggttcga
      721 gtcgaagaga ccggggcagg tcaggcccat gccggcggag ggagcctgat aggcgaaggt
      781 aacccgcgag tagttgaagc cgccgaccac cgcgggctgc tcctcggtca ccggtttgat
      841 cgcggagccg tccgcgtcgt cactcgcgtc ccagttgtca tcgaccctta ccccggtcac
      901 ctgccggccc gggttgtgct tgaccagcag ggtgatccgg atggtattgc cgtcccggcc
      961 cgccccggtg acggtgccca gaccgtcgtc cacggtgttg tcgctgatcg tcccgctctc
     1021 cagccaggaa cgctggagga ctccggtgtc gacttccacg ttcgtgtcgt tgacgatggt
     1081 cggtggaatg tcatcggggc cgttcgggtc gacccagttc tccgaatgcg gggccggatt
     1141 gaagcgcata gccaaagtgg ccttgttctc acccgaagga gaaggcaggc ccatggtcga
     1201 gttgacgatc tggttgacgt cctggcccac gatcgtcggg caaccggacg cggccgggat
     1261 cgccagggtg tcgtcgacga ccgtggcggt cgccgggtac tcgtcatagg ggacgcctcc
     1321 ggtggtgtcg ctcgaagcgg agaagggaat cgggctggcc ggggtgccta cgtagcagtt
     1381 gttgcccagg gcgacgatcg cgtggttgct ggtcaactgg atcgtcaggt cggtcgaaag
     1441 ggaaacctcg ccggtcaccg ggttgatcga accggtgacg ggagaggcgg cgcggatctg
     1501 gacgaagatg tccaccgcgc cgaccgggct
//
LOCUS       DAOTIP010000584         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_97669_length_1530_cov_3.150508, whole genome shotgun sequence.
ACCESSION   DAOTIP010000584 DAOTIP010000000
VERSION     DAOTIP010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97669_length_1530_cov_3.150508"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="P5138_10080"
     CDS             <1..604
                     /locus_tag="P5138_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60967.1"
                     /translation="AEGRRGFVFLGRDGPTPYGPGEVTLGARSGSARADSDFRFRGST
                     LEFGRDYLGTRIGLDPIDDGIAEKAEKFQLVLSNPQGDAALSDATSSITVSIPASKNA
                     TLKLGRLGLNRKKGTGTLTARVSSPGRVSVSGKGLVRSTKKLTSAGKVVLPLKATGKA
                     KRTLRKTGQVTMKAKVTYRTLGGRKVTRTRSFSLKSGGHH"
     gene            complement(668..>1530)
                     /locus_tag="P5138_10085"
     CDS             complement(668..>1530)
                     /locus_tag="P5138_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60968.1"
                     /translation="LPMECGDTDHAPQYSTGIALDRAADNTISDNDFEDVTYGVRVED
                     DGTKVTGNSFSGTDAGDYAVMIGTPYRTSLLEQPVSGTELIDNESTIEGNQSPYRWVE
                     GEADTTVSGNTALGEEAGICQSASIPRGPFVMTIAVKPEADPLPPDPLAIPTVGTQEA
                     CPQDPPPDEPDDPEPPVVPSNQFQFGKVSLNRKNGSGMIGVKLPGAGKLRLQGTGAVK
                     PQARTVGGAKTVKLTIAARGKAARNLRRKGRVRVKVLVSFTPKGGKARTKSRMVTLVR
                     KKPRHHAGRR"
BASE COUNT          243 a          516 c          492 g          279 t
ORIGIN      
        1 ggccgaaggg cggcgcggtt tcgtcttcct cggccgcgac ggcccgactc cctacggtcc
       61 gggggaggtc accctcggcg cccggtcagg ctccgcccgg gcggacagcg atttcagatt
      121 ccggggttcc accctcgagt tcggccggga ctaccttgga accagaatcg gcctggatcc
      181 cattgacgac ggaatcgccg agaaggccga gaagttccaa ctggtcctat cgaacccgca
      241 aggtgatgcg gccctttcgg acgcgaccag cagcatcacc gtgtcgatcc cggcctcgaa
      301 gaacgcaacc ctgaagttgg gcagactcgg gctcaaccgg aagaagggaa ccggaaccct
      361 gaccgccagg gtttcgtccc cgggcagggt ttccgtctcc ggcaagggtc tggtccgctc
      421 gacgaagaag ctcacgagcg cgggcaaggt tgtcctgccc ctgaaggcga ccgggaaggc
      481 aaaacggacc ctccggaaaa ccggccaggt gacgatgaaa gccaaagtca cctaccgcac
      541 gctgggtgga cggaaggtca cccggacccg gtcgttcagc ctcaagtcag gcggacacca
      601 ctgagccgac ctgccggtcg ccgtccccgg aacgagccgt gaccaagacc gcgacggctg
      661 ccggaagcta gcgccgcccg gcgtgatgcc gcggcttctt cctgaccagg gtcaccatgc
      721 gggacttcgt cctggctttg ccgcctttcg gagtgaaact gaccagcact ttcaccctga
      781 ccctgccctt tcgcctgagg ttccgggccg ccttgcctct cgcggcgatc gtcagtttga
      841 cggtcttcgc ccctcccacc gtcctggcct gcggcttgac cgccccggtg ccctggagcc
      901 ggagcttgcc ggcgcccgga agcttcaccc cgatcatgcc gcttccgttc ttcctgttca
      961 gcgagacctt tccgaactgg aactgattcg aaggcacgac cggcggttcc gggtcgtcgg
     1021 gctcgtccgg gggcggatcc tgtggacagg cctcctgggt gcccacggtc gggatcgcca
     1081 gggggtccgg cggcagggga tcggcctccg gcttgaccgc aatcgtcatc acaaatgggc
     1141 cgcggggaat agaagcgctc tggcagattc ccgcctcttc gccgagcgcc gtgtttccgg
     1201 agacggtggt gtccgcctcg ccttcaaccc agcggtaggg actctggttg ccctcgatcg
     1261 tcgattcgtt gtcgatcaac tcggttccgc tgacgggttg ctcgagcagg ctggtccggt
     1321 aaggggtgcc gatcatcacc gcgtagtcgc cggcgtcggt accgctgaaa ctgtttccgg
     1381 tcaccttcgt gccgtcgtcc tcgaccctca ccccgtaggt cacgtcctcg aagtcgttgt
     1441 cgctgatcgt gttgtcggca gcgcggtcaa gggcgatgcc ggtcgaatac tggggggcgt
     1501 ggtcggtgtc cccgcattcc atcggcaaga
//
LOCUS       DAOTIP010000585         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_98416_length_1522_cov_3.832993, whole genome shotgun sequence.
ACCESSION   DAOTIP010000585 DAOTIP010000000
VERSION     DAOTIP010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98416_length_1522_cov_3.832993"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="P5138_10090"
     CDS             <1..348
                     /locus_tag="P5138_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV60969.1"
                     /translation="SALSHLYEKQQRDQLSGVVGSYEVTRQAVDIGAREALTLIALIS
                     LSLGIINLFPFLPLDGGHIFWSLVEKVRGRPVPFAVMERAGLIGFALVIGLFLLGLNN
                     DIGRLTGEGFNVR"
     gene            460..>1522
                     /locus_tag="P5138_10095"
     CDS             460..>1522
                     /locus_tag="P5138_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV60970.1"
                     /translation="MQTSETVTEFDGGPAIEPEFKNLYEAFQATVARKGDSLAIKSED
                     EGVELSWNDLDKRVRKIAGGLNALGVKRGDRVGMLLGSRADFIPIDLAAVSIGALPFS
                     IYQTLSPDQIEYVVEDSGTELVITESAYLPNLLEARKNLPGLKRVVMLDGEGGDMTMD
                     ELEAMDPDFDPGPLAAEVELDDPLTLIYTSGTTGPPKGVLLTQGNLMSMLTTVMHSVL
                     EIPDEGRVISWLPAAHIAERGANYYTPVMHGLEVHVCPDPRRIIEFLPKVKPSWFFAV
                     PRIWEKLKAGLESNLASLPEEQREQALAGLNAAIQKVRLEQAGEEVPEELAAAVAAAD
                     EALFSNLRVALGFDEALAVS"
BASE COUNT          291 a          465 c          491 g          275 t
ORIGIN      
        1 tccgcgctgt ctcaccttta cgagaaacag cagcgtgacc agctctccgg tgttgtcggc
       61 agctacgaag tcacccggca ggcggttgac atcggggccc gcgaggcgct cacgctgatc
      121 gccctgatca gcctctcgct cggcatcatc aacctctttc cgttcctgcc gctcgacggc
      181 ggtcacatct tctggagtct cgtcgagaag gtacgcggca ggccggtgcc attcgcggtg
      241 atggaaaggg cagggctgat cggatttgcc ctcgtcatag gcctattcct gctcggcctg
      301 aacaatgaca taggccgcct aaccggcgaa ggattcaacg tgagataggg agtagtcccg
      361 gtcggagacg cctctagggg aagtccccta tttgcgttct tccctgactt ggatacattc
      421 ctgtggcagc gctttgggaa gagagctaag gaggagcagg tgcagacgtc cgagacggtg
      481 accgaattcg acggcggtcc cgctattgag ccggagttca agaacctcta cgaggccttt
      541 caggccacag ttgcccgcaa gggtgattcg cttgcgatca agtccgagga cgaaggggtc
      601 gaactcagct ggaacgacct tgacaagcgg gtacggaaga tcgcgggcgg cctgaatgcg
      661 ctcggagtga agcgaggcga ccgggtcggc atgctgctcg ggtcacgggc cgatttcatc
      721 ccgatcgacc ttgcggctgt ctccatcggg gcgctcccct tctcgatcta ccagactctc
      781 tcgccggatc agatcgaata cgtggtcgag gattcgggca cggaactggt catcaccgaa
      841 agcgcatacc tgccgaacct gctcgaggcc cgcaagaacc tgcccgggct gaaacgggtc
      901 gtcatgctcg acggagaagg cggcgacatg accatggacg agctcgaggc gatggacccg
      961 gacttcgacc ctggcccgct ggcggcagaa gtggaactgg acgatccgct gaccctgatc
     1021 tacacctcgg gtacgaccgg cccgcccaag ggagtgctgc tgacccaggg caacctgatg
     1081 tcgatgctga ccacggtcat gcattcggtg ctggagatcc ccgacgaggg tcgcgtgatc
     1141 tcctggctgc ccgcggccca catcgccgaa aggggagcca actactacac gcccgtcatg
     1201 catgggctcg aggttcatgt ctgcccggat ccgcgacgga tcatcgagtt cctcccgaag
     1261 gtgaagccat cctggttctt cgcggttccc cgcatctggg agaagctcaa ggccggcctg
     1321 gaatcgaacc tggccagcct gccggaggag cagcgcgagc aggcgctggc cggactgaac
     1381 gccgcaatcc agaaggtccg gctcgaacag gccggagaag aagtgccgga agagctggcc
     1441 gcagctgtgg ccgcagccga cgaggcgctg ttttcgaatc tccgggttgc cctcggcttc
     1501 gacgaggcgc ttgcggtgag cg
//
LOCUS       DAOTIP010000586         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_98739_length_1519_cov_2.297131, whole genome shotgun sequence.
ACCESSION   DAOTIP010000586 DAOTIP010000000
VERSION     DAOTIP010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98739_length_1519_cov_2.297131"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1519)
                     /locus_tag="P5138_10100"
     CDS             complement(<1..>1519)
                     /locus_tag="P5138_10100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRV60971.1"
                     /translation="DGSTQDITPGGSEPDFVEEDPNLNGGVGPGSNVDTGPTTSDVLT
                     VPNFVIDQFEIPPFLLPIYQACGSQYGIPWYVLASINRTETYFGTNVATSSAGANGWM
                     AFMPASWAEWGVDANGDKVRDPYNPVDAICSAANYLKYFGYEKSPYDAIFAYNHADWY
                     VQLIFKYAKVYAQIPPEVISALTGLTEGARFPVAAADASYEGEVSTEAAKKHGTASQD
                     IQSSADRRTIAISASGGSPVIAVNDGVISDIDQETGTVVLEDAYGNRYSYAGLGSVST
                     VHPVPQPEPSSPIAPDESDSATTTSGDQGQPDPEAEMGEQKSKAEPTPAEQEALGSNP
                     QAKDEGSSKPTKKEQQDISEVLVDPNAADAAAAAATEQAASGQPADPAVTSADDRRAE
                     QDDVRQVPAEADNNTEDSRGRVYASPLRPSNQHKASTDGQSLQNDVRQAEGVGIDGKP
                     GDYLIYDGSKAGIYRFDPDTTELRELKKGSRVIAGTVLGRLAETPVASVNFSIKPG"
BASE COUNT          194 a          462 c          561 g          302 t
ORIGIN      
        1 caccgggctt gatcgagaag ttcaccgagg cgaccggggt ctcggcgagc cgcccgagca
       61 cggtaccggc gatcacccgc gatcccttct tcagttccct tagctcggtc gtgtcgggat
      121 cgaagcggta gatgccggct ttgctgccgt cgtagatcag gtagtcgccc ggcttgccgt
      181 caatgccgac gccctcggcc tggcggacat cgttctgcag ggactggccg tcggtgctgg
      241 ccttgtgctg gttgctcgga cgcagcgggc tggcatagac ccggccccgg ctgtcctcgg
      301 tgttgttgtc ggcctcggcc ggcacctggc ggacgtcgtc ctgttcggcc cggcggtcgt
      361 cggctgacgt gacggcggga tcggccggtt ggccggaggc ggcctgttcg gtggcggcgg
      421 ccgccgccgc atcggcggca ttggggtcga ccagcacttc gctgatgtcc tgctgttcct
      481 tcttcgtggg tttcgatgaa ccctcatcct tagcctgagg gttagacccg agggcttcct
      541 gctcggccgg agtcggttcg gcctttgact tctgctcgcc catctcggct tccgggtcgg
      601 gctggccctg gtcgccgctc gtggtcgtcg cggagtccga ttcgtcaggg gcgatcggcg
      661 aggaaggctc cggctgcggt accgggtgga ccgtcgacac cgagccgagg ccggcgtagg
      721 agtaacggtt gccgtaggcg tcctcgagca cgacggtccc ggtctcctgg tcgatgtcgg
      781 agatcacacc gtcgttgacc gcgatcaccg gcgagccgcc ggaggcgctg atcgcgatgg
      841 tgcggcggtc ggccgaagac tggatgtcct gggaggcggt gccgtgcttc ttcgcggctt
      901 cagtcgagac ctcgccttcg taggaagcgt ctgccgcggc aaccgggaag cgggcgccct
      961 cggtgaggcc ggtcagcgcg ctgatcacct cgggcgggat ctgggcgtag accttggcgt
     1021 acttgaagat cagctggacg taccagtcgg cgtggttgta ggcgaagatc gcgtcgtagg
     1081 ggctcttctc gtagccgaag tacttgaggt agttggccgc ggagcagatc gcgtcgaccg
     1141 ggttgtacgg gtcgcggacc ttgtcgccgt tggcgtcgac gccccactct gcccaggagg
     1201 cgggcatgaa ggccatccag ccgttggcgc cggcggagga ggtggcgacg ttggtgccga
     1261 agtaggtctc ggtccggttg attgaggcga gcacgtacca cgggatcccg tactggctgc
     1321 cacaggcctg gtagatcggg agcaggaacg gcgggatctc gaactggtcg atgacgaagt
     1381 tgggaacggt caggacatcc gaggtggtcg ggccggtgtc cacgttgctg ccgggcccga
     1441 cccctccgtt caggttcgga tcctcttcga cgaagtccgg ctcgctgccg ccgggggtta
     1501 tgtcctgggt gctgccgtc
//
LOCUS       DAOTIP010000587         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_99187_length_1514_cov_3.141193, whole genome shotgun sequence.
ACCESSION   DAOTIP010000587 DAOTIP010000000
VERSION     DAOTIP010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99187_length_1514_cov_3.141193"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1025)
                     /locus_tag="P5138_10105"
     CDS             complement(219..1025)
                     /locus_tag="P5138_10105"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRV60972.1"
                     /translation="MSSGAPAPGTGIPVRTLPNLRDLGGYATAAGSRVKHGQLYRSVL
                     LGRLSDDDVGKVRELGLKTVFDFRTAAEFEASPDRDVGARMINLDVLADRTGEGPASL
                     LAKMNDPEAVKAALTDGRGAELMRTAYRELIDLPSARQAYGTFFDDLASGDPFPALFH
                     CTTGKDRTGWAAAVTLLFLGVDEEDVYRDYLLTNEQLLPALKPILTHIEEAGIEFDLL
                     RPVIGVDRSYLETAIDLMKEQYGSIDGYMSEGLGLGDEVLEVLRDRLIEA"
     gene            complement(1022..>1514)
                     /locus_tag="P5138_10110"
     CDS             complement(1022..>1514)
                     /locus_tag="P5138_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HRV60973.1"
                     /translation="RLTIDVRGTGSTGYHIAELARDLAGVHLELASDTVAVAVFGMGT
                     GTPQRARRLIDGILSALEAIEAEPVQEKPEFAPPPLWGDPAMTPRDAFLGEQEVVPFS
                     EAEGRIAAEPLATYPPGIPNVLPGERLTRETLDYISDSVDHGGYVRGAADRELTTLRV
                     IAE"
BASE COUNT          249 a          494 c          497 g          274 t
ORIGIN      
        1 gagcttcccg ccctcttcca gatccccgga gccctcaagg atgaaggggt tccattcggg
       61 ataggaccgg aagtcggtga ggatttccca gaccctttcc ggcggggcgt cgatttctgc
      121 gcttgtctga atcttcggca tggatagagg ctaacacagt tagccaagaa tggcaaacag
      181 cgttagcctc cggtccgcac cggatccggc cgccggactc aggcttcgat caaccggtca
      241 cgaagcactt cgaggacctc gtctccgagt cccagacctt cgctcatgta accgtcgatc
      301 gagccgtatt gctccttcat gaggtcgatc gcggtctcca ggtatgaacg gtccacacct
      361 atgaccggac gcaacaggtc gaactcgatg cccgcttcct cgatgtgggt gaggatcggt
      421 ttcagggcgg gcagcaactg ctcgttggtc agcaggtagt cccggtagac atcctcctcg
      481 tccacgccca gaaagagcag ggtcacggca gcggcccagc cggtccggtc cttgccggtg
      541 gtgcagtgga agagggcggg gaaggggtcg ccgcttgcca ggtcgtcgaa gaaagttcca
      601 tacgcctgcc gggccgaagg aagatcgatc agttcccggt aggccgtccg catcagctcg
      661 gcgccccggc cgtcggtcag ggctgccttg accgcttcag gatcgttcat ctttgccagc
      721 aggctggccg gaccttcacc ggtccggtcg gccagcacgt ccaggttgat cattcgcgcg
      781 ccgacgtcgc gatccgggga agcttcaaac tccgccgcgg tccggaagtc gaagaccgtc
      841 ttcagaccga gttcccggac cttcccgacg tcgtcatcgc tcagcctgcc cagcagcacc
      901 gaacggtaga gctgcccgtg tttgactctt gaaccggcgg cggtcgcata accgccgagg
      961 tcccgcaggt tgggaagggt tctgaccggg attccggttc ccggggcggg agctccggaa
     1021 ctcattcggc gatgacccga agggtggtca gttcccggtc ggcggccccc cggacatacc
     1081 ctccgtggtc gaccgagtcc gagatgtagt cgagcgtttc ccgggtcagc ctctcgccgg
     1141 gaagcacgtt ggggatgccg ggcggatacg tggccagcgg ctccgccgcg atccggcctt
     1201 ccgcttcgga aaagggcacg acctcctgct cgccgaggaa ggcatcacgc ggggtcatcg
     1261 cggggtcgcc ccagagtggc ggcggtgcga actccggctt ctcctggact ggttcggcct
     1321 cgatcgcctc cagggcgctg aggatgccgt cgatcaggcg ccgggcgcgc tgcggggttc
     1381 cggttcccat cccgaacacc gccaccgcga ccgtgtcgga ggccaattcg aggtggacac
     1441 cggcaaggtc ccgggccagc tcggcgatgt ggtagcccgt gctgccggtg ccccgcacgt
     1501 cgatggtcag ccgg
//
LOCUS       DAOTIP010000588         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_99741_length_1508_cov_3.114935, whole genome shotgun sequence.
ACCESSION   DAOTIP010000588 DAOTIP010000000
VERSION     DAOTIP010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99741_length_1508_cov_3.114935"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="P5138_10115"
     CDS             <1..259
                     /locus_tag="P5138_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60974.1"
                     /translation="SGIAEIAGGAAMLPDSTAKPAGLWLTALLLAVFPANIHMAIDRG
                     QIERMARSGIPKWALYARLPLQPVLIYLVLKSTRSGTQQVV"
     gene            complement(236..1075)
                     /locus_tag="P5138_10120"
     CDS             complement(236..1075)
                     /locus_tag="P5138_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60975.1"
                     /translation="MRPDEPTDEMPGGADEPTEVNTPGDEPTGRIPEGNENPTDVIPE
                     GNENPTNVLGGEPEEPTEVAPGPRGPVRPPAGQPKGGNRGIAVLVGSIVASLILVGAY
                     VAAGGPVGPDKAADPCDARPWTDPQNLEESAQQFALSAADGAACELGVSREELTRAIA
                     DDKALQGFMDENGFSESDMEDALRAGLNRAVDDAENAGALSALAATGLRAAVKVMPMS
                     VMIGLLENADSIFNGDLGGLGDLGGLGDLGDALNAITGGVENLGTGTTDDPLQGLNDL
                     LGP"
     gene            complement(1072..>1508)
                     /locus_tag="P5138_10125"
     CDS             complement(1072..>1508)
                     /locus_tag="P5138_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60976.1"
                     /translation="HGRSSAAVHGGWTISARHAGVVVGLLILTPIFTHQLDVQKEAAL
                     DAGTAIVLDSPIDPSDKIDLGEKLAKEVDLQGDRVPDLSPAFEPMPTDPEVRSQYETI
                     LSGIEVQLKDAATKAFSLSFLISALFGLLALIPIGMTRRLDL"
BASE COUNT          228 a          527 c          488 g          265 t
ORIGIN      
        1 cagtggcatc gcggagatcg cgggcggggc ggcgatgctg cctgactcga ccgcgaaacc
       61 ggccgggctg tggctcacgg cgctgctcct tgcggtcttc ccggccaaca tccacatggc
      121 gatcgaccgg ggccagatcg aacgcatggc aaggtccgga atccccaaat gggcgctcta
      181 cgcccggctg ccgcttcagc cggtgctcat ctatctcgtc ctgaaatcga ccaggtcagg
      241 gacccagcaa gtcgtttagc ccctgcagcg gatcgtcggt ggttcccgtt ccgaggttct
      301 cgacgccgcc cgttatcgcg ttcagggcgt ctccgaggtc gccgagcccg ccaaggtctc
      361 ccagcccgcc aaggtccccg ttgaagatgc tgtccgcgtt ctccagcaag ccgatcatca
      421 ccgacatcgg catcaccttg accgcggcgc gcaggccggt cgcggcgagg gcactgagcg
      481 cgccggcgtt ttcggcgtca tcgaccgccc ggttgagccc ggcccgcaac gcatcctcca
      541 tgtcggactc ggagaatccg ttctcgtcca tgaagccctg aagcgccttg tcgtcggcga
      601 tcgcccgggt cagctcctcc cggctcacgc ccagctcgca ggccgccccg tctgcggccg
      661 aaagggcgaa ctgctgcgcc gactcctcga ggttctgggg gtcggtccag ggacgggcgt
      721 cacaggggtc ggccgccttg tccggtccga ccgggccacc ggccgcgacg taggcaccga
      781 caaggatcag ggaggcgacg atcgatccga ccagtacggc gatgccacgg ttgccgccct
      841 tcggctggcc tgccggcggt ctgaccgggc cgcgcggacc gggcgcgacc tcggtcggtt
      901 cctccggctc accgccaagt acgttggtcg ggttctcgtt gccctcgggg atcacgtcgg
      961 tcgggttctc gttcccttcc gggatcctgc cggtcggctc gtcgcccggg gtgttcacct
     1021 cggtcggttc atccgccccg cctggcatct cgtctgtcgg ctcgtccggc ctcacaggtc
     1081 aagcctccgg gtcattccga tcgggatcag cgccagcaag ccgaacaggg ccgagatcag
     1141 gaaggaaagc gaaaacgcct tcgtggccgc gtctttcaac tggacttcga tcccggagag
     1201 aatcgtttcg tactggctcc gaacttcggg gtcggtcggc atcggctcga aggccggcga
     1261 aaggtccggc acccggtcgc cctgaaggtc gacctccttt gccagtttct cgccgagatc
     1321 aatcttgtcg gaggggtcga tcggagagtc gagcacgatc gccgtccctg catccagggc
     1381 cgcctccttc tggacatcga gctgatgggt gaagatcggc gtgaggatca gcagtccgac
     1441 cacgactccg gcgtgacggg ccgaaatcgt ccagccgcca tgaaccgccg cagacgaccg
     1501 gccatgca
//
LOCUS       DAOTIP010000589         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_100238_length_1503_cov_2.834254, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000589 DAOTIP010000000
VERSION     DAOTIP010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100238_length_1503_cov_2.834254"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="P5138_10130"
     CDS             <1..626
                     /locus_tag="P5138_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60977.1"
                     /translation="SQKADAQTELTAITREVESGRTELDDSLKKAAAAIAAAEEKASQ
                     AEARLKRETAAGREAAAEWVRGQAAEIEADAALAAEMSDEGQAPATSGEPDPVLSARV
                     TSLEAELAAEKAAKDEALTKAAARLKEIEESARLAEARVEAAETAADEARNKAEVQAA
                     AAPAGDGKAATEAEAREAAVNWLRGQISALRQEIAKSDEAGNDPGGS"
     gene            626..913
                     /locus_tag="P5138_10135"
     CDS             626..913
                     /locus_tag="P5138_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60978.1"
                     /translation="MGLFSRGGQTGRERRAMQDHLRELDDMRRNNLGDLGQMTVEMAS
                     EGRFDRSRLSQQAAEVAKIDREADLIARGLEEGLTLQELEDLARGDGATSG"
     gene            935..>1503
                     /locus_tag="P5138_10140"
     CDS             935..>1503
                     /locus_tag="P5138_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60979.1"
                     /translation="MPASSEERDLGTFDLTVVGGGAAGLWAALCAAEEGGSVCLVSRK
                     PLAESASFHAQGGLAASLDPSDSPEQHFEDTINAGRNLCRASAVRVLVDEAPGIVDDL
                     IRRGMNFDREADGSLSLALEGGHSQRRIIHSGGSQTGREITSALAHMVARMDQVTVLE
                     ETSAVALISYGDRCHGVITERGAITSAATL"
BASE COUNT          296 a          513 c          519 g          175 t
ORIGIN      
        1 cttcacagaa ggccgatgcg cagaccgaac tgacggcgat cacccgcgag gtcgagtccg
       61 ggcgaaccga actcgacgat tccctgaaga aggcggccgc tgcgatcgcc gccgccgaag
      121 agaaggccag ccaggcagag gcccggctca agcgcgagac cgccgcaggc cgtgaagccg
      181 ctgccgaatg ggttcgcggc caggccgcgg agatcgaggc cgacgccgcc ctcgccgccg
      241 agatgagcga cgaaggacag gccccggcga caagcggcga gcccgacccc gttctcagcg
      301 ccagggtcac ttcgctggaa gccgaactgg ccgccgagaa ggccgcaaag gatgaggccc
      361 tgaccaaggc cgcagcccgg ctcaaggaaa tcgaggagag cgcccggctg gcagaggccc
      421 gtgtggaagc agccgaaacc gctgccgacg aggccaggaa caaggccgag gtccaggcag
      481 cggcagcccc ggccggcgac ggcaaggcag cgaccgaggc cgaagccagg gaagccgccg
      541 tcaactggct gcgagggcag atttcggcgc ttcgccagga gatagccaag tccgacgaag
      601 ccggaaacga tccgggaggc agctgatggg actcttcagt cgaggcggac agaccgggcg
      661 cgaacgccgt gccatgcagg accacctccg cgagctcgat gacatgcgcc ggaacaacct
      721 cggcgatctc ggccagatga ccgtggagat ggcctccgag gggcgcttcg accgcagccg
      781 gctgtcccag caggcagccg aggtggcgaa gatcgaccgg gaagctgacc tgatcgcgcg
      841 cgggctcgag gagggcctca ccctccagga gctggaagac ctagcccgcg gcgacggggc
      901 cacttccggc tagatgcgaa cccggtactg acgattgccc gccagctccg aagagcggga
      961 cctcgggact tttgatctga cggtagtcgg cggtggtgcc gccgggctct gggccgcgct
     1021 ctgcgccgcc gaggaaggtg gatcggtctg cctggtctca cgcaagccgc tcgccgagag
     1081 cgccagcttc catgcccagg gcgggctggc ggcgtcgctc gatccctccg attcacccga
     1141 acagcacttc gaagacacga tcaacgccgg tcgcaacctc tgccgcgcct ccgccgtgag
     1201 agtcctggtc gatgaagccc ccgggatcgt cgacgacctg atcaggcggg gaatgaactt
     1261 cgaccgggaa gcggacggtt cactctccct cgcgctcgaa ggcggccaca gccagcgtcg
     1321 gatcattcac tccggaggca gccagacggg ccgggagatc acctccgccc tcgcccacat
     1381 ggtcgccagg atggaccagg tcacagtcct cgaggaaacc tcggcggttg ccctgatcag
     1441 ctacggcgac cgctgccacg gagtgatcac cgaaaggggc gccatcacca gcgccgcgac
     1501 cct
//
LOCUS       DAOTIP010000590         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_101133_length_1493_cov_3.637691, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000590 DAOTIP010000000
VERSION     DAOTIP010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101133_length_1493_cov_3.637691"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="P5138_10145"
     CDS             <1..336
                     /locus_tag="P5138_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV60980.1"
                     /translation="AFAAMPNLIMEVVPVQRTGEATGFNTLIRSVGSSLGSQVTASIL
                     TGTILLATGFPTSEGFRTAFLVGAAVSIFAGVLALLIPRSGHRHAIVSEEMGAASMLP
                     EPALAADEK"
     gene            333..929
                     /locus_tag="P5138_10150"
     CDS             333..929
                     /locus_tag="P5138_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV60981.1"
                     /translation="MTPAGTGTSVEAPAERAGRKRADAVRNREKVIAAAEQVFAEQGI
                     EAGIPEIAERAGVGKGTVYRNFETKDDLVAAILVRRMTRFDEDIIEALDSDDPGAAFR
                     DALRKGAARASDLSFPAVMYWTGKSPELDETKAKVRDHMTRLVRKAKKAGAIRKDAKV
                     DEIWTLFGGVCRSLGDAGEADPKVWRRHADLVADAFRA"
     gene            complement(960..>1493)
                     /locus_tag="P5138_10155"
     CDS             complement(960..>1493)
                     /locus_tag="P5138_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HRV60982.1"
                     /translation="EIASRRAETVAKIREIAPIEFVNGGGTGSLESTSAEDAVTEVAA
                     GSGFFAPGQFDHYAAFTLRPAAGYALPVVRRPGPGVVTALGGGYLASGPMDPGRMPVP
                     WLPAGLSFEKDEGAGEVQTPLVGDAADSLKIGDNVYLRHAKAGELCERFASLILIEGD
                     EIVDEVPTYRGEGQTFL"
BASE COUNT          249 a          503 c          519 g          222 t
ORIGIN      
        1 gccttcgcgg cgatgccgaa cctgatcatg gaggtcgttc cggtccagcg caccggcgag
       61 gcgaccggct tcaacacgct gatccgatcg gtcggttcct cgctcggatc ccaggtgacg
      121 gcgtcgatcc tcaccgggac gatcctgctg gctaccggct ttccgaccag cgagggtttc
      181 cgcactgcgt tcctcgtcgg ggctgcggtc tcgatcttcg ccggcgttct cgcgctcctg
      241 atcccgcgct ccggccaccg gcacgcgatc gtgtccgagg agatgggcgc agcgagcatg
      301 ctccccgaac ccgccctggc ggcagacgag aaatgactcc ggccgggacc gggacttccg
      361 tcgaggcacc ggccgaaagg gcggggcgga agcgggcgga cgcggtccgc aaccgggaga
      421 aggtgatcgc cgccgccgag caggtgttcg ccgagcaggg gatcgaagcg gggatccccg
      481 agattgccga gcgggccgga gtcggcaagg gcaccgtcta caggaacttc gagaccaagg
      541 atgatctggt cgccgcgatc ctggtccggc ggatgacccg tttcgacgaa gacatcatcg
      601 aggccctcga cagcgacgac ccgggagccg ccttcaggga tgcgctgcgg aagggtgccg
      661 cccgggccag cgacctgagc tttccggcgg tcatgtactg gaccggcaag tcgccagagc
      721 tggacgagac aaaggcaaag gtcagggatc acatgacgcg gctggttcgc aaggcaaaga
      781 aggcgggcgc gatccgcaaa gacgcgaaag tggatgagat ctggaccctc ttcggtggcg
      841 tctgccgcag ccttggtgac gcaggggagg cggacccgaa agtctggcgc cgccacgccg
      901 atctcgtcgc cgacgctttc cgcgcctgac cgattgcccg gagaccgggc agccggaact
      961 cagaggaagg tctggccctc gccgcggtag gtgggaacct cgtcgacgat ttcgtcgccc
     1021 tcgatcagga tcagcgaagc gaagcgttcg cacaactcgc cggccttggc gtgccggagg
     1081 tagacgttgt cgccgatctt cagggagtcc gcggcgtcgc cgaccagtgg ggtctggacc
     1141 tccccggcgc cttcgtcctt ctcgaacgag agtcccgcgg gcagccaggg aacgggcatc
     1201 cgtccgggat ccatcggccc cgaggccagg tagccgccac ccagcgccgt gaccaccccg
     1261 gggcccggcc gcctgaccac cggcagtgcg tagccggccg ccggcctgag cgtgaacgcg
     1321 gcgtagtggt cgaactgtcc cggagcgaaa aagccggagc ccgccgcgac ttccgttacg
     1381 gcatcctcgg ccgaggtcga ctcgagcgag ccggtgccgc cgccgttcac gaactcgatc
     1441 ggggcgatct cacggatctt cgccaccgtt tcggccctcc tcgaggcgat ctc
//
LOCUS       DAOTIP010000591         1489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_101554_length_1489_cov_2.855649, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000591 DAOTIP010000000
VERSION     DAOTIP010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101554_length_1489_cov_2.855649"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="P5138_10160"
     CDS             complement(<1..339)
                     /locus_tag="P5138_10160"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRV60983.1"
                     /translation="MIIGRPHLHFRLTDSTNERARQAAADGAPSGTLITAESQTAGRG
                     RQGRPWSTPAGSAVAWSVLIRQSVEVPGTLPLQIGVGVCEAVESLGATGVRMKWPNDI
                     WIEDRKCAGIL"
     gene            586..888
                     /locus_tag="P5138_10165"
     CDS             586..888
                     /locus_tag="P5138_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60984.1"
                     /translation="MIDVEYRAWISIPNFPSAEGDAWEPFIDYMEAAHGEFGPVLGWE
                     SGRLANLVMSTDAESPTEAAQVLFAAAADGLRAVDLVDLYPSAVKIELAEDEPVPA"
     gene            complement(885..>1489)
                     /locus_tag="P5138_10170"
     CDS             complement(885..>1489)
                     /locus_tag="P5138_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRV60985.1"
                     /translation="PGVGSSRRAVVLTCGNHHLVGPDNGLLSLAATVLGGVEAAFDVS
                     GTPVRAGDSDTFHGRDVFSPVAAELARGAAPGELGDPVGFRELVPLELPEPVITPEQV
                     DAHVLYPDQYGNVVLNLHEEVLDGTFFTEVDSVQLETSLQETIVPLGRTFADVEAGEP
                     VIYTDSTGYLALAVNRGSAASRFGLGPDNQLRFTPAGRNA"
BASE COUNT          260 a          466 c          500 g          263 t
ORIGIN      
        1 caggatcccg gcgcattttc ggtcttcgat ccagatgtcg ttgggccatt tcatccgtac
       61 gccggtcgca cccagtgact cgaccgcttc acagaccccg acgccgatct gaaggggcag
      121 cgtgcccggc acctcgaccg actgccggat caggacgctc caggccaccg ccgatccggc
      181 cggtgtgctc cacggccgac cttgcctccc ccggcccgcg gtctggctct cggcggtgat
      241 cagggttccg gatggagccc catccgcggc ggcctgacgg gcccgctcgt tggtcgaatc
      301 ggtcaggcgg aaatgcagat gtggacggcc aatgatcact cgccggaggc taccggggag
      361 gtgagtcttt gccgctcatc ccttatcggg tcatgagtta cagcggcgat ctccggaaat
      421 tgatcaggag tttgcgaaag caaggctggc gcgttgagaa acgccgcaag cactgggtgg
      481 cattcccccc gaatcgggac ctgagtccgg agattctggc gacgacgccg agcagccaga
      541 ggacctggcg aaatcaattg gccaaactga gagcgaaggg ctatcgtgat tgacgtggag
      601 taccgagcct ggatcagcat cccgaatttt ccgtcggcag aaggagatgc ttgggagcca
      661 ttcatcgact acatggaggc ggcccacggc gagttcggtc ccgtgctcgg atgggaatca
      721 ggcaggctgg caaacctcgt gatgtcgact gacgcggaaa gtccgaccga ggccgcccag
      781 gttctcttcg cggctgccgc agatggtctt cgtgccgtag acctggtcga tctttacccg
      841 tcggcggtga agatcgaact ggctgaggac gaaccggtac cggcctaggc gtttcggcct
      901 gccggggtga acctgagctg attgtcgggc ccgaggccga agcggctggc ggcgctgcca
      961 cggttaaccg ccagggcgag atagccggtc gagtcggtgt agatgaccgg ctctcccgct
     1021 tccacgtcgg cgaaggtgcg accgagcggc acgatggtct cctgcaggct ggtttccagc
     1081 tggacagaat caacttccgt gaagaaggtt ccgtcgagga cctcctcgtg caggttgagg
     1141 acgacgttgc cgtactggtc cgggtagagg acatgggcat ccacctgttc cggagtgatg
     1201 accggttcgg gcagctccag cggcaccagt tcacggaaac cgaccgggtc gcccagttca
     1261 cccggcgccg caccccgggc cagttcggca gccaccgggc tgaagacgtc gcggccgtgg
     1321 aaggtgtcgc tgtctccggc tcgcaccggg gttccggaca cgtcgaaggc cgcctccact
     1381 ccgccgagca cggtcgccgc gagggaaaga agtccgttgt cgggtccgac gaggtggtga
     1441 ttcccgcagg tcagcacgac cgccctccgg ctgcttccga ccccggggt
//
LOCUS       DAOTIP010000592         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_101643_length_1488_cov_3.230984, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000592 DAOTIP010000000
VERSION     DAOTIP010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101643_length_1488_cov_3.230984"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="P5138_10175"
     CDS             complement(<1..530)
                     /locus_tag="P5138_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV60986.1"
                     /translation="MSRTDRPPSACDIAVAGGGIIGLAVARELALREPDARICLLEAE
                     DRLAAHQSSHNSGVVHAGVYYTPGSLRAKLCVEGAARLEEYCAERKLPWRASGKLIVA
                     TTEEELPGLDELERRGRTNGVKGLARVKAAEIAGIEPAARGLSALHSPFTAVTDFASI
                     AASYADDFEAAGGSIHL"
     gene            complement(616..1200)
                     /locus_tag="P5138_10180"
     CDS             complement(616..1200)
                     /locus_tag="P5138_10180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRV60987.1"
                     /translation="MKVLAIVGSLRENSFSEQVARAAGDLAPEGVEVEVYEGLASVPA
                     YNADHDPAYDGPEPVPAEVEDLRNQISAADALLVVTPEYNAGPPGYLKNAIDWASRPH
                     KASSLAGKPAAIVSQSPMPFGGIWANQALRKSFTITGTPTVERELAIGKVDEKIEDGR
                     LTDDQARADVIALMAELNELVAVIANPDEEAVAA"
     gene            complement(1203..>1488)
                     /locus_tag="P5138_10185"
     CDS             complement(1203..>1488)
                     /locus_tag="P5138_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRV60988.1"
                     /translation="TFVSTGVSKSDAETYEVTGDLTLRGVTNPVTLTVEVEGAGIGMD
                     GSSVLSLRADGEINRNDYGISWGASLDNGAAVVAEKVRLVLTVEAVKAGE"
BASE COUNT          218 a          495 c          508 g          267 t
ORIGIN      
        1 aggtggatgc tgccaccggc cgcttcgaaa tcgtcggcgt aggacgcggc gattgaggcg
       61 aagtcggtga ctgcggtgaa cggggagtgg agggcagaaa gcccgcgggc agccggttca
      121 atcccggcta tttcggctgc tttcacacgc gccagcccct tgactccgtt ggtccggccc
      181 cggcgctcga gctcgtcgag cccgggcaac tcttcttcgg tcgtggcgac gatcagcttg
      241 ccgctggccc gccagggaag tttccgttcg gcgcagtatt cctccagccg cgcggctcct
      301 tccacgcaga gcttcgcccg cagcgatccg ggcgtgtaat agaccccggc gtggaccacc
      361 ccggaattgt gggaggactg gtgtgcggcg agccggtcct ctgcctcgag caggcagatc
      421 cgcgcatcgg gctcgcggag ggcgagctca cgggcgacgg cgagtccgat aatgccgccg
      481 cccgccaccg cgatgtcgca tgcggatggc gggcggtcgg tccggctcat tcgccgatta
      541 tcggcgaaag gccggtatcc aaacggcggt tggtcggatt accgctccat gtcccgagga
      601 tccgacccac cgggttcagg cggcgacggc ttcctcgtcc gggtttgcga tcacggcgac
      661 cagctcgttc agttcggcca tcagggcgat cacgtccgcc cgcgcctgat catcggtcag
      721 gcggccgtcc tcgatcttct cgtcgacctt tccgatcgcg agttcgcgct cgacggtcgg
      781 ggttccggtg atcgtgaacg acttccgcag cgcctgattg gcccagatcc cgccgaaagg
      841 catggggctc tgcgagacga tcgccgccgg cttgccggcc agggaagaag ccttgtgagg
      901 cctcgacgcc cagtcgatcg cgttcttcag atagccggga gggcccgcgt tgtattcggg
      961 ggtgacgacc agcagggcat cggccgcgct gatctggttc cgcaggtcct cgacttccgc
     1021 cggtaccggc tccggcccgt cataggccgg gtcgtggtcg gcgttgtagg ccgggaccga
     1081 agccaggcct tcatagacct cgacttccac gccctccggg gcgaggtcac ccgcggcgcg
     1141 ggcgacctgc tccgagaagg agttctcgcg caggcttccg acgattgcca ggaccttcat
     1201 ggttactcgc ccgccttgac ggcctcgacc gtgagaacga gacggacctt ctcggcgacc
     1261 acggcggcgc cgttgtcgag cgaggcgccc caggagattc cgtagtcgtt gcggttgatc
     1321 tcgccgtctg cccggaggct gaggaccgag ctgccgtcca taccgatacc cgcgccctcg
     1381 acctcgacgg tcagggtgac cggattggtc actccgcgca gggtgaggtc accggtcacc
     1441 tcataggtct cggcgtcgga cttgctgacg ccggtggaga cgaaggtc
//
LOCUS       DAOTIP010000593         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_101963_length_1485_cov_2.135664, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000593 DAOTIP010000000
VERSION     DAOTIP010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101963_length_1485_cov_2.135664"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="P5138_10190"
     CDS             <1..243
                     /locus_tag="P5138_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRV60989.1"
                     /translation="IEQLEDLGLVRKVDLGASAHGYEKVEPSGHHHHHIVCDDCGKVE
                     PFEDEALEEAIHHIRRKGFSLESHEVTLHGHCAECR"
     gene            complement(222..1106)
                     /locus_tag="P5138_10195"
     CDS             complement(222..1106)
                     /locus_tag="P5138_10195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRV60990.1"
                     /translation="MKQLKKDRSARVAAGSNRPVFIKVGDSNTATHLALYGLGCYRYK
                     PKGLRSSLIKVVRRYRRTSLASGRLDVPGASCRNGNPDNSFSRISLASRGGAYFSYAL
                     EPGETYGLPCAGSILVCEIEATGPRYAFIQFGTNEALQAPEGDMSAGQLEEMRAQISE
                     IVRACRSYRVTPVILTAPVALDGGNWTPGVPGRVVAINQMIRQTAKASRAPLIDLWLA
                     QSEVIGPRFNYGLDKGGIHLSSSPNTDPWRGTVNLSILNRRRYGMNLRSYLILSALEK
                     LDSIKPRRGGRPQRHSAQ"
     gene            complement(1251..>1485)
                     /locus_tag="P5138_10200"
     CDS             complement(1251..>1485)
                     /locus_tag="P5138_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRV60991.1"
                     /translation="AVLPDIRSVGVMGDERTYAYPIVIRAVTSEDAMTADWARLPYEL
                     LERMSSRIINEVPGVNRVAYDITSKPPGTIEWE"
BASE COUNT          268 a          484 c          469 g          264 t
ORIGIN      
        1 atcgaacagc tggaggatct gggcctggtc cggaaagtcg acctcggcgc ctcggcccac
       61 ggctacgaga aagtcgagcc ctcggggcac catcatcacc acatcgtctg cgacgactgc
      121 ggcaaggttg agcccttcga agacgaagcc cttgaagagg ccatccatca catccgtcgc
      181 aagggcttta gtctcgaatc acacgaagtg accctccacg gtcactgcgc cgagtgccgc
      241 tgagggcgtc cgccccggcg tggcttgatc gaatcgagct tttccagagc cgagaggatc
      301 agatagctgc gcaggttcat tccgtaccgg cggcgattga gaattgacag gttgaccgtg
      361 ccccgccacg ggtcggtatt cggagacgag gagaggtgaa tcccgccctt gtccagcccg
      421 tagttgaacc ggggcccgat cacttccgac tgggcaagcc agagatcgat caggggcgcc
      481 cgactggcct tcgccgtctg gcggatcatc tggttgatcg cgaccacgcg gcccggaaca
      541 ccgggcgtcc agttgcctcc gtcgagggcc accggcgcag taaggatcac cggagtaacc
      601 cggtatgacc ggcaggcccg gacgatttcc gagatctggg cccgcatctc ctcgagctgc
      661 ccggcggaca tatccccctc cggggcctga agagcttcgt tggttccgaa ctggatgaag
      721 gcgtaacggg gaccggttgc ttcgatctcg cagaccagga tcgatcccgc gcaggggagt
      781 ccgtaggtct cgccgggctc cagggcatag ctgaaataag cccctccccg cgaagccagg
      841 gagatccggc tgaatgagtt gtccggattc ccgttccggc agctcgcgcc gggaacgtcg
      901 agccgtccgg acgccaggct ggttcgcctg taccgcctga ccaccttgat cagggaactg
      961 cgaagcccct tcggcttgta ccggtaacaa cccagcccgt acagggccag atgcgtggcc
     1021 gtgttcgagt cgccgacctt gatgaagacc ggcctgttgc tgccggcagc gacgcgggcg
     1081 ctccggtcct tcttcagctg tttcacggtg gctgcgggaa accgcaggta gtccggaatc
     1141 tgcccacgcg aagccagggc gcccccactc ccgaaaacaa gcacagcgat tgttgtcatt
     1201 cccgtagcta ttgccctcat ggcaagtccg tcctggatgg agagttctat ctactcccac
     1261 tcaatggtgc cgggcggctt cgacgtgatg tcgtaggcca cgcggttgac gccgggcacc
     1321 tcgttgatga tccggctcga catgcgctcg agcagctcgt agggcaggcg cgcccagtcg
     1381 gcggtcatcg cgtcctcgct ggtcaccgca cggatcacga tcgggtaggc gtaggtgcgc
     1441 tcgtcgccca tcacgcccac cgaccggatg tcgggcagca ccgcg
//
LOCUS       DAOTIP010000594         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_102120_length_1483_cov_2.411064, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000594 DAOTIP010000000
VERSION     DAOTIP010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102120_length_1483_cov_2.411064"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1483
                     /locus_tag="P5138_10205"
     CDS             111..>1483
                     /locus_tag="P5138_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60992.1"
                     /translation="MREIKRFMAVAAALITVFALSSAASASQNPPGCAGTTPIASFTD
                     ETLGQISDPVREGDEIIFSALFSNRGESACNLSGVAISVILPKPDGTPGEELPLTNNL
                     SLPEGFDARITNHNPWVVDLDEGVFEAPIGLTWRATSHTGDPDAQISGAGPGTKLTIT
                     RPEVNLKVSSSPASGPSPLFATTTYKLTNTSPSAAGGLPAPGLVPASQDGSRNVLSDE
                     ACGPLKYVSGDLPSDEAPALDPGETWVFTCQRSYLLPGVHSAQPTVTGASDMDGRSWP
                     QPSADLEAGAVKVLGSDLTVEKSHTGDLLAGSSGLYELVVTNSGNQDSSGTVTVADQL
                     PAGLDPVSIAGPGWNCDLASLSCSRSDVLGPGSSFPPVTVTVDVANDPPESVVNKATV
                     SGGGEPPAATINNSFDDPTTIRTPGQPAMPVSNRFSVSRVKTRGNGSVVILVSVPGAG
                     RIWIDDA"
BASE COUNT          270 a          511 c          467 g          235 t
ORIGIN      
        1 cgatcgacga agagggcgag ccggttcacc aggccgagtc cgaggcctga agcaacgctc
       61 ccgcggccga cgctcctgcc gctactcttt agttgaaggc aaggggttcg atgagggaga
      121 tcaaacggtt catggcagtg gcggcggcac tgatcacggt gttcgctctt tcgtctgcgg
      181 ccagcgccag ccagaatccg cccggctgcg cgggaacgac cccgatcgcg agtttcacgg
      241 atgaaaccct cggtcagatc tcggatccgg tacgggaagg ggacgagatc atcttctcgg
      301 ctctcttttc caaccgcggg gaatcggcct gcaacctcag cggcgtggcg atcagcgtca
      361 tcctgccgaa gccggacggg acccccggcg aggagcttcc gctcaccaac aacctgagcc
      421 tgccggaggg cttcgacgcc cggatcacca accacaatcc ctgggtggtc gacctggacg
      481 agggcgtctt cgaggcgccg atcggcctga cctggcgggc tacttctcac accggagacc
      541 cggacgcgca gatcagcggc gccggaccgg gcacaaagct gaccatcacc cgccccgagg
      601 tcaatctcaa ggtttcaagc agcccggcgt cgggaccttc ccccctcttt gccacaacga
      661 cctacaagct gaccaacacc agtccctccg ccgccggcgg cctccccgcc cccgggctgg
      721 tcccggcttc ccaggacggg tcccggaacg tcctctccga tgaagcctgc gggccgctga
      781 aatacgtctc gggcgacctc ccctccgatg aagctccggc cctggatccg ggcgagacct
      841 gggtcttcac ctgccagcga agctacctgc tgcccggtgt ccattcggcc cagccgaccg
      901 tcacgggagc gagcgacatg gacggaaggt cctggcctca gccgtcggcg gatctcgaag
      961 ccggagccgt gaaggtactc ggctccgatc tgacggtcga aaagagccat accggagacc
     1021 tgctcgccgg cagctccggc ctgtatgagt tggtggtgac caactccgga aaccaggatt
     1081 caagcggcac ggtgacagtc gcggaccagc tgcccgcggg cctcgacccc gtttccatag
     1141 ccggcccggg ctggaattgt gatctggcaa gcctcagctg cagtcgatcc gatgtgctcg
     1201 gcccggggag ctccttcccg cccgtgacgg tgaccgtgga cgtcgccaat gatccacccg
     1261 aaagcgtcgt gaacaaggcg acggtgagcg gcggaggcga gcctccggcg gcaacgatca
     1321 acaactcctt cgatgatccg accacgatcc gcacaccggg ccagccggcc atgccggtct
     1381 cgaaccgttt ttccgtatcc agggtgaaga caaggggcaa cggcagcgtc gtgatcctgg
     1441 tttcggtgcc cggcgcgggc cggatctgga ttgacgacgc gaa
//
LOCUS       DAOTIP010000595         1481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_102310_length_1481_cov_2.595372, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000595 DAOTIP010000000
VERSION     DAOTIP010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102310_length_1481_cov_2.595372"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="P5138_10210"
     CDS             complement(<1..434)
                     /locus_tag="P5138_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRV60993.1"
                     /translation="MRIASLVPSSTEMLFALGFGDQVVAVTHECDFPPEAAALPHLSE
                     TVIEPGLSPAEIDAEVKRVVGQGDPLYRLKAEVLESVRPDLIIAQDVCAVCAVSYDDV
                     LAIAARLPGNPVVLKQDPTSLVEVLGNAIEVAEATGNPEAGVA"
     gene            469..747
                     /locus_tag="P5138_10215"
     CDS             469..747
                     /locus_tag="P5138_10215"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRV60994.1"
                     /translation="MALLEDQEIQDRLAELDGWSLEGRRIVRTFEFDDFVGSINFVDS
                     LVQPAESMNHHPDLGISWNKVTVAITNHAEGGLTEKDFELASRIQALV"
     gene            complement(744..>1481)
                     /locus_tag="P5138_10220"
     CDS             complement(744..>1481)
                     /locus_tag="P5138_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRV60995.1"
                     /translation="KVVMLVRSEESADRALTAVEKASRRIPGANPSRVTTTMEIADLA
                     EANLLVEAVVEDAGVKADLLRRISEVAPRADLATTTSSLSITKLGEDSGAADRVYGLH
                     VFNPVPAMQLVELIFPDPVTEEVAERAHDWCHILEKTAVEVPDTAGFAVNRLLFPYLF
                     DAVRYQERTGLEAAAVDQCMTLGVAHPMGPLALLDLVGLDVAVAIGQALNNESGNPDH
                     IAPATVQEFVAAGHLGRKTGQGFYEYG"
BASE COUNT          264 a          443 c          513 g          261 t
ORIGIN      
        1 gcaacgccgg cttcggggtt gccggtcgct tccgccactt cgatcgcgtt gcccaggacc
       61 tccaccaggg aggtcgggtc ctgcttgaga accaccggat tgccgggcag gcgggcggcg
      121 atcgccagca catcgtcgta agagacggcg cagaccgcgc agacatcctg ggcgatgatc
      181 aagtccggcc ggaccgattc cagtacctcg gccttgaggc ggtagagcgg gtcaccctgg
      241 ccgaccaccc gcttcacttc ggcgtcgatc tcggccgggg aaaggcccgg ctcgataacc
      301 gtctcgctga ggtgggggag agcggcagcc tcaggcggga agtcgcactc atgggtgacg
      361 gccacgacct ggtcgccgaa gccgagcgcg aagagcatct cggtggaaga aggaacgagg
      421 gaggcaatcc tcatgggtga aatatggcag cccgggtagg ctcggaccat ggcgctgctt
      481 gaggatcagg aaatacagga ccgcctggct gaactcgatg gctggagcct cgagggccgg
      541 aggatcgttc gaacgttcga gttcgacgac tttgtcggct cgatcaactt cgtcgattcg
      601 ctggttcagc cggccgaatc gatgaaccat caccccgacc tcggtatctc ctggaacaag
      661 gtcaccgtgg cgataaccaa ccacgccgaa ggcggcctca ccgagaaaga cttcgagctg
      721 gcctcaagga tccaggcgct ggtttagccg tattcgtaga aaccctggcc ggttttgcgg
      781 ccgaggtggc ctgcagcgac gaattcctgg accgtggcgg gggcgatgtg gtccgggttt
      841 cctgattcgt tgttgagtgc ctggccgatc gctaccgcga catcgaggcc gaccaggtcg
      901 agcagggcga gggggcccat ggggtgggcg accccgaggg tcatgcattg gtcgaccgcg
      961 gcagcctcca ggccggtgcg ctcctggtag cggactgcgt cgaagaggta ggggaagagc
     1021 agacggttga ccgcgaaacc ggctgtgtcg ggcacctcga cggcggtctt ttcgaggatg
     1081 tggcaccagt cgtgggctcg ctcggctacc tcctcggtaa ccgggtcggg aaagatcagt
     1141 tcgaccagtt gcatcgccgg gacggggttg aagacatgga gtccgtagac ccggtcggcg
     1201 gcccccgaat cctcgcccag cttcgtgatc gaaagggagg aagtggtggt tgcgagatcg
     1261 gcccgcgggg cgacctcgga aattcgtctc agcagatccg ccttgacccc ggcgtcctcg
     1321 acaactgctt cgaccaggag gttggcttca gccaggtccg cgatctccat ggtcgtggtc
     1381 acccgtgagg ggttggctcc ggggattctc cggctggcct tttcgacggc ggtcagggcc
     1441 cggtccgccg actcttcgga acgaaccagc atcacgacct t
//
LOCUS       DAOTIP010000596         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_102380_length_1480_cov_3.247719, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000596 DAOTIP010000000
VERSION     DAOTIP010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102380_length_1480_cov_3.247719"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="P5138_10225"
     CDS             complement(<1..369)
                     /locus_tag="P5138_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60996.1"
                     /translation="MGIPGALRNVGGSSKDLAARQTRPPAAIALPAVFAVLFLILPLA
                     AVVIRLPWGSVGEILTRSDTVEALALSLGTASVATVLCVIFGVPLAWWLDWLGRSPRS
                     LVYRLTRTLVLVPLVLPPVVG"
     gene            complement(348..1118)
                     /gene="modA"
                     /locus_tag="P5138_10230"
     CDS             complement(348..1118)
                     /gene="modA"
                     /locus_tag="P5138_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV60997.1"
                     /translation="MAGRSLLAILATLAVAVALPACGSSDDSGSGDKEDLTVFAASSL
                     TEAFTNLGKQYEAAHEGAVVKFNFGSSSDLAAQIDQGAPADVFASADEPNMQKVVDSD
                     MADGEPQDFASNVLEIAVPRDNPGKVTGLDDFAEKDLKLAVCDPEAPCGNAATEVFGN
                     AGVDASIDSYEPDVKSVLTKVELGEVDAGLVYHSDVLAAKDRIDSIAIPEQDQFINVY
                     PIVVVKGAGNAQGGQDFIDLVLGDDGQNELAKWGFRAP"
     gene            complement(1120..>1480)
                     /locus_tag="P5138_10235"
     CDS             complement(1120..>1480)
                     /locus_tag="P5138_10235"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRV60998.1"
                     /translation="ALLGVSDDTIRRWAADGRLTLREGKGGVKVVDGRELARLASEST
                     LEAEGGATFSVRNRMHGIVTAVKTDAVMAQVDIQAGPFRLVSLISSEAVEQLGIKVGK
                     AVFATAKATNVGVEIAS"
BASE COUNT          262 a          493 c          469 g          256 t
ORIGIN      
        1 gccgaccacc gggggcagca ccagcgggac caggacgaga gtcctggtaa ggcgataaac
       61 caggctgcgg ggtgagcggc cgagccagtc gagccaccag gccaggggta ctccgaagat
      121 cacgcagaga acggtcgcga ccgacgccgt gccgagcgaa agggccagcg cctcgacggt
      181 gtcgcttcgg gtgaggatct ccccgaccga accccagggc aacctgatga ccaccgccgc
      241 caggggcagg atcaaaaaga gaaccgcgaa aaccgccggc agcgcgatcg cggccggcgg
      301 cctggtctgg cgggccgcca ggtccttcga ggacccgccg acgtttctca gggcgcccgg
      361 aatccccact tcgccagttc gttctgaccg tcgtcgccga gcacgaggtc gatgaagtcc
      421 tggccgccct gggcgttgcc ggcacccttg accacgacga tcgggtagac gtttatgaac
      481 tggtcctgct cgggaatcgc gatcgagtcg atcctgtctt tggccgcgag gacatccgag
      541 tgatagacga gcccggcatc gacctcaccg agttctacct tggtcagcac cgatttcacg
      601 tcgggctcgt aactgtcaat cgaagcgtcg actccggcgt tcccgaagac ctccgtggcg
      661 gcgtttccgc agggagcttc ggggtcgcag accgccagtt tcaggtcctt ttcggcgaag
      721 tcatcgagac cggtcacctt gccgggattg tccctgggga ccgcgatctc gaggacgttg
      781 ctggcaaagt cctgcggctc gccgtcggcc atgtccgaat ccacgacctt ctgcatgttc
      841 ggctcgtcgg ccgaggcgaa gacgtcggcc ggcgcgccct ggtcgatctg ggcggcgagg
      901 tccgagctcg acccgaagtt gaacttgacc accgcgccct catgcgcggc ttcgtactgc
      961 ttgccgaggt tggtgaaggc ttcggtcagc gaggatgccg cgaagacggt cagatcctcc
     1021 ttgtcaccgg aacccgaatc atccgaactg ccgcaggccg gaagcgctac cgcaacggct
     1081 agcgtcgcga ggatcgccag aaggctcctt ccggccaatt tacgacgcaa tttccactcc
     1141 cacgttggtt gcttttgcag ttgcgaacac ggccttgccg accttgatgc cgagctgctc
     1201 gactgcttcg ctgctgatca gcgaaacgag ccggaaggga ccggcctgaa tgtcgacctg
     1261 agccattact gcgtcggtct tgaccgcggt cacgatcccg tgcatgcggt tgcgaaccga
     1321 aaacgtcgca ccgccttcgg cctccagcgt tgattccgag gccagccggg caagttcgcg
     1381 gccatcgacg accttgaccc cgcccttccc ctcccggagg gtcaaccggc cgtctgcggc
     1441 ccagcggcgg atcgtgtcat cgcttactcc gagcagggca
//
LOCUS       DAOTIP010000597         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_102487_length_1479_cov_3.169944, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000597 DAOTIP010000000
VERSION     DAOTIP010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102487_length_1479_cov_3.169944"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="P5138_10240"
     CDS             complement(<1..1007)
                     /locus_tag="P5138_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV60999.1"
                     /translation="MLVAAFVSLLIAAPASAYVLGPYPGNPITIPNSGSGSPYPSTLS
                     LGSVIGKLDSMTAQVNVSHEFAGDIDLLLVSPQGVGVMLMSDTCGSQTISSMSFTFSD
                     SAASMLPETATCTSGTYKPTDYVSSTQDTFPSPAPSSYSPTLSAYKGIDPTGNWSLFV
                     VDDAAGDTGAINSWSLTFDVSPAEIKVPEFGTSGKAEPYPSTRTVNTPPGRVISDLKL
                     NIQGFGHSSPGDVDMLLADDKGNAAVVMSDSCGSDPFYGRNWTFGDPFVDALGESGPC
                     PDGNYQTTDYLTAPDDQWPAPAPAPQFGNFSDVFGGLEGGLWSLYVVDDSEGNYGYIN
                     G"
     gene            1168..1473
                     /locus_tag="P5138_10245"
     CDS             1168..1473
                     /locus_tag="P5138_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV61000.1"
                     /translation="MDEATVGGWNPDPLPRGRHRLSADYVRASQRERLLRAMLELVAE
                     NGYQVASVPDVVTRARVSRSSFYQEFDGKLGCFLALCDEYSRGMIDEVLVRDSKTKK"
BASE COUNT          300 a          426 c          475 g          278 t
ORIGIN      
        1 catccgttta tgtacccgta gttcccttcg ctgtcgtcaa ccacatagag actccagagc
       61 cctccctcca ggcctccgaa gacgtcgctg aagttcccga actgtggtgc gggagccggg
      121 gccggccact gatcatcggg ggcggtcagg taatcggtgg tctggtaatt cccgtccggg
      181 cagggtccgg actcaccgag agcgtccacg aacggatcgc caaaggtcca gttccttccg
      241 tagaagggat cactcccgca ggagtccgac ataacgacgg cggcgttccc cttgtcgtcg
      301 gcgagcagca tgtctacgtc cccgggactc gaatggccga aaccctggat gttcagtttg
      361 agatccgaga tcacccggcc cggcggggtg tttaccgttc tggtcgaagg gtatggctcg
      421 gccttgccgg aagtgccgaa ttcgggaacc ttgatctcgg ccggggacac atcgaaggtg
      481 aggctccagc tattgatcgc ccccgtatcg ccggcggcgt cgtcgacgac gaagaggctc
      541 cagttcccgg tcggatctat gcccttgtat gccgacagcg ttggcgaata cgaactcggg
      601 gccggtgagg gaaaggtgtc ctgtgtcgag ctgacgtaat cggtcggttt gtaggtgccg
      661 gaggtgcagg tagccgtttc cgggagcatt gacgcggccg agtcgctgaa ggtgaaggac
      721 atgctggaaa tggtctggct gccacaggtg tcggacatga gcatcacgcc gaccccctgg
      781 ggtgagacaa gcaacaggtc tatgtcaccg gcgaattcgt ggctgacatt cacctgggcg
      841 gtcatgctgt cgagcttccc gatgacgctc cccagcgaca aggtggacgg atagggcgat
      901 ccggagcccg aattcggaat cgtgatcggg tttcccggat acggcccaag cacgtatgcc
      961 gatgcaggcg cggcgatgag cagcgagacg aacgcggcaa ccagcaacga aagtgaaaat
     1021 cgaaaacctg ggaagaacat cagcggcttg cccccgcggg atctggaatc tgttccgaga
     1081 ctagcgacct ggcccccatg catcaaaggt tccgttgccg aagggaagtt cgactcccga
     1141 ccgtgtagcg cctctagaat cccggacatg gacgaagcga ccgtgggggg ctggaatccc
     1201 gatccgctgc cgaggggaag gcaccgcctg tcggccgatt acgtccgtgc ctcacagagg
     1261 gaacggctgc tccgggcgat gctcgaactg gttgccgaga acggctatca ggtggccagc
     1321 gtgccggacg tcgtgacccg ggcccgggta tccagatcgt ccttctacca ggagttcgac
     1381 ggcaagctcg gctgcttcct tgccctttgc gacgaatatt cgcggggcat gatcgatgag
     1441 gttctcgtca gagattcaaa aaccaaaaag taataacaa
//
LOCUS       DAOTIP010000598         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_102676_length_1477_cov_3.354430, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000598 DAOTIP010000000
VERSION     DAOTIP010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102676_length_1477_cov_3.354430"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1477)
                     /gene="acnA"
                     /locus_tag="P5138_10250"
     CDS             complement(<1..>1477)
                     /gene="acnA"
                     /locus_tag="P5138_10250"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRV61001.1"
                     /translation="PSIAGPKRPQDRVSLSDAKPAFLEALCEFDEEASNQLSAYDEAL
                     EESFPASDPPAENESDGDSRPHVVEPNEADIAVAMERCSEDKIPVTLEDGTSFDLDHG
                     RVVIAAITSCTNTSNPYVMVGAGLLARNALAKGLERKPWVKTSLAPGSTVVTDYLDNA
                     GLTQYLDDLQFNLVGYGCTTCIGNSGPLAPEISAAIDEKDLVVCSVLSGNRNFEGRIS
                     QDVKANYLASPPLVVAYALAGRMDIDIQKDPLGNDPDGNPVYLDDIWPDPEEVARVVG
                     ESIRREMFEKNYGDVFKGDVNWQQVSVPEGDRYTWPDSTYVRRPSFFEDMPADPPGLA
                     PVEGARVLAKLGDSITTDHISPAGAIKKDSPAGAWLIEQGVGPADFNSYGSRRGNHEV
                     MVRGTFANIRLRNQLVERSGGYTRHYPDGEETSIYEASSQYQADGIPLVVLAGKEYGS
                     GSSRDWAAKGTRLLGVRAVIAESYERIHRSNLIGMGVVPLQY"
BASE COUNT          237 a          459 c          517 g          264 t
ORIGIN      
        1 ggtactggag cgggaccacc cccatgccga tcaggttgga gcggtggatc cgctcgtagc
       61 tctcggcgat caccgcccgg acgccgagca gcctggtgcc cttggcggcc cagtcacgcg
      121 atgaacccga gccgtactcc ttgccggcca ggaccaccag cgggatgccg tcggcctggt
      181 actggctgga agcctcgtag atggaggtct cctccccgtc ggggtaatga cgggtgtagc
      241 caccggagcg ctcgaccagc tggttgcgga gccggatgtt ggcgaaagtg ccgcgcacca
      301 tcacctcgtg attgccgcgg cgggaaccgt aggagttgaa gtcggccggg ccgacgccct
      361 gctcgatcag ccaggctccg gccgggctgt ccttcttgat cgcgccggca ggcgagatgt
      421 ggtcggtggt gatcgaatca ccgagcttgg ccagcacgcg agccccctcg accggcgcca
      481 ggccgggcgg gtcggccggc atgtcctcga agaatgaagg ccggcggaca taggtcgaat
      541 ccggccaggt gtaacggtcg ccttccggca ccgagacctg ctgccagttc acatcgccct
      601 tgaagacgtc gccgtagttc ttctcgaaca tctcgcggcg aatcgactcg ccgaccaccc
      661 gggcgacctc ctccgggtcg ggccagatgt catcgagata gaccgggttc ccgtccgggt
      721 cgttgccgag cgggtccttc tggatgtcga tgtccatgcg tccggcgagg gcgtaggcga
      781 ccaccagcgg aggcgaggcg aggtaattgg ccttgacgtc ctggctgatc ctcccctcga
      841 agttccggtt gccggaaagg accgagcaga cgaccaggtc cttctcgtcg atcgcggcgg
      901 agatctccgg ggcgagcggg ccggagttgc cgatgcatgt cgtacacccg taaccgacaa
      961 ggttgaactg gaggtcgtcc aggtattggg tgagacctgc gttgtcgagg tagtcggtga
     1021 ccacggtcga gcccggcgcc agcgaggtct tcacccaggg cttccgttca agacccttgg
     1081 ccagggcgtt gcgggcgagc aacccggctc caaccatcac ataggggttc gaggtgttgg
     1141 tgcaggaggt gatcgcggcg atcacgaccc ggccatggtc gaggtcgaag ctggttccgt
     1201 cctcgagagt aaccggaatc ttgtcctcgc tgcagcgctc catggcgacc gcgatgtcgg
     1261 cttcgttcgg ttccacgaca tggggacgcg aatctccgtc gctttcgttt tcggccggcg
     1321 ggtcggaggc cgggaaggac tcctcgagcg cctcgtcgta ggcgctgagc tgattcgagg
     1381 cttcctcgtc gaactcgcag agggcttcga ggaaggcggg cttggcgtcc gagagcgaga
     1441 cccggtcctg gggacgcttg ggaccggcga tcgaagg
//
LOCUS       DAOTIP010000599         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_103150_length_1472_cov_4.179958, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000599 DAOTIP010000000
VERSION     DAOTIP010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103150_length_1472_cov_4.179958"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="P5138_10255"
     CDS             <1..292
                     /locus_tag="P5138_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61002.1"
                     /translation="GEDDSAGDDTETDGSEAEGRPRRRRRGRRGRRGRGPGNKLEGTF
                     DHGDEGFGLWLDPAIQDIQAYSENWAGKRDVTVTVEADQIIIRRAGEPADES"
     gene            complement(289..618)
                     /locus_tag="P5138_10260"
     CDS             complement(289..618)
                     /locus_tag="P5138_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61003.1"
                     /translation="MAYPLENALFNWEAGIDRLRELERSGQLRGDDVTVPVREELRRR
                     LGATFTASDLASLYGEGTDWAVMLPGVDPGQSDVQELIDAAFWLHLQSAGDYAGGRVV
                     DLELEET"
     gene            complement(645..>1472)
                     /locus_tag="P5138_10265"
     CDS             complement(645..>1472)
                     /locus_tag="P5138_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61004.1"
                     /translation="TFAINLRFLIPALGLAVVLVPLSSWFDRGWRRYALGVLLVVLFI
                     FTSASDPVISAPGRTFGVAIALLFVAMPFAVWLLRERLSPHLRLPPLAVGLGLAIAVF
                     AVFAWPIQKSYFEDRYQDFELADGSGLTAPYRWAEGITDSKIALAGTTAGFKGYGFWG
                     RDLSNDVVYIGRDASHGGFDAIGNCGEFARAVNAADPDYLVTSPFLNFNEYSRPIASP
                     ENAWAENDPALVKVMPRVKPSHANRPVTVWKVTGPMDPSLCARLGAGSDFVPGLKDG"
BASE COUNT          282 a          473 c          521 g          196 t
ORIGIN      
        1 gggcgaggac gattcggccg gtgacgacac cgagacggac ggctccgagg ccgaaggccg
       61 gccccgccgg cgtcgccgcg gacgtcgggg acgccgcgga cgcggtcccg gcaacaagct
      121 cgaaggcacc ttcgaccacg gtgacgaggg cttcgggctg tggctcgacc cggccatcca
      181 ggacattcag gcctacagcg agaactgggc cgggaagcgc gacgtgacgg tcacggtcga
      241 agcggaccag atcatcatcc gccgcgccgg cgagccggcc gacgagtcct aggtctcctc
      301 cagctcaaga tccaccaccc tgccgccggc gtagtccccg gcggactgaa ggtgaagcca
      361 gaaggcggcg tcgatcagtt cctggacgtc tgactgtccc gggtccactc ccggcagcat
      421 cacggcccag tccgttcctt ccccgtagag ggaagcgaga tccgacgctg tgaacgtcgc
      481 gccgagccgc cggcgcagtt cctcccgcac cggcaccgtc acatcatcgc cccgcaactg
      541 accggagcgt tcgagctccc gcagccggtc gataccggct tcccagttga agagggcgtt
      601 ctcgagggga taggccacgc gcagatgatg gcagtccgtg gagctcagcc gtccttgagg
      661 ccggggacga agtcggaacc ggcaccgagc cgggcgcaaa gggagggatc catcggcccg
      721 gtcaccttcc agaccgtcac cggcctgttc gcatgcgacg gcttgacccg gggcatgacc
      781 ttgacgagag cggggtcgtt ttcggcccag gcgttttccg gggatgcgat cgggcgcgag
      841 tactcgttga agttcaggaa aggcgaggtg accagatagt cgggatcggc cgcgttcacg
      901 gcgcgggcga actccccgca gttgccgatc gcatcgaagc ctccatggga agcgtcccgg
      961 ccgatgtaaa ccacgtcgtt cgaaaggtcc cggccccaga agccgtaccc cttgaaaccg
     1021 gccgtcgtgc cggccagggc gatcttcgag tcggtgatgc cctcggccca gcggtacgga
     1081 gcggtcaggc cggagccgtc cgcgagttcg aagtcctggt agcggtcctc gaagtagctc
     1141 ttctggattg gccaggcgaa gacggcgaag acggcgatgg ccagtccgag gccgacggcc
     1201 aatggtggca gcctgaggtg tggcgaaagg cgctcacgca gcagccagac cgcgaagggc
     1261 atggccacga agaggagggc gatcgccacg ccgaaggtgc ggccaggggc agagatgacc
     1321 ggatcggagg cgctggtgaa gatgaaaagc acgaccagca gcactccgag cgcgtagcgg
     1381 cgccagccac ggtcgaacca ggaggaaagc ggaaccagca cgaccgccag acccagggcc
     1441 gggatcagga agcggaggtt gattgcgaag gt
//
LOCUS       DAOTIP010000600         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_103152_length_1472_cov_3.815808, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000600 DAOTIP010000000
VERSION     DAOTIP010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103152_length_1472_cov_3.815808"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="P5138_10270"
     CDS             complement(<1..231)
                     /locus_tag="P5138_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61005.1"
                     /translation="MNEAGRIDGFDRTLGRIGAASADGYGIIVRRRKGPGDGWFSASE
                     LDDSGRVQALLLSRISGMAGPPEDHIRAEWLLE"
     gene            complement(236..1162)
                     /locus_tag="P5138_10275"
     CDS             complement(236..1162)
                     /locus_tag="P5138_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61006.1"
                     /translation="MLRLLKTLPGLIVILAGLAVPSLAQAFDAGVRAPLLTDRAPGAV
                     SVPFEIPFRQSAAVPQPLEFEVAVQGFSLAPAAIDGTRIGSLDMVTDAGDFDDKAIYS
                     DGPGADGTRTWTLDWQLSNDPITATVTDGTGLDASGEKINAPDSTLITFTVPGNYHGF
                     RLMGLSLRFNQEDHGMPTPGVGAVNPVMPGNYLIRSRIRSVAPQSAVKVASTTVPIGL
                     EAPKTRLVASANRFRVRRGGKVRISMRTANQTRDTVGIWLRGKRLRKVKVGPATKHIY
                     WRPGRKFRGRRAVLHLRPAHGPDRQLVIRVIR"
     gene            1274..>1472
                     /locus_tag="P5138_10280"
     CDS             1274..>1472
                     /locus_tag="P5138_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61007.1"
                     /translation="MQIENFLFSVSDALRWPVLVAVLLALAWSVIEIGILLAEVWRRR
                     WRSINALENAVDQAEIDIVQGD"
BASE COUNT          227 a          487 c          490 g          268 t
ORIGIN      
        1 ttccaggagc cattccgccc ggatgtgatc ttccggcggg ccggccatgc cgctgatcct
       61 ggagagaagc agcgcctgga cccggccgct gtcgtcgagt tcgctggcgg agaaccagcc
      121 gtcacccggg cccttccgcc ggcggacgat gatcccgtag ccgtcggccg aggcggctcc
      181 gatccggccg agggtccggt cgaaaccgtc gatccggccg gcttcgttca tctgctcagc
      241 ggataacccg gatcaccagt tggcgatctg ggccatgcgc gggtcggaga tgtaagaccg
      301 ccctgcggcc cctgaacttc cggcccggtc gccagtagat gtgtttggtg gcgggcccga
      361 ccttgacctt tcgcagccgc ttgccgcgga gccagatgcc gaccgtgtcc cgggtctggt
      421 tcgcggtccg catcgagatc ctgaccttcc cgccccgtct cacacggaac cggttcgcgc
      481 ttgcgaccag tctggtcttc ggtgcttcca gaccgatcgg gaccgtggtc gaagcaacct
      541 tcaccgcgct ctgcggggca acggaccgga tccggctgcg aatcaagtag ttgcccggca
      601 tcaccggatt gacggcaccg acgcccgggg tcggcatccc gtggtcttcc tggttgaacc
      661 gcaggctcaa acccatcagc cggaagccgt ggtagttgcc gggaacggtg aaagtgatca
      721 gggtcgagtc cggcgcgttg atcttctccc cggaggcatc gaggccggtc ccgtctgtga
      781 ccgtggccgt gatcggatcg ttgctgagtt gccagtcgag ggtccaggtc ctcgtcccgt
      841 cggcgcccgg cccgtccgag tagatcgcct tgtcatcgaa atcaccggcg tcggtgacca
      901 tgtcgaggga accgatccgg gttccgtcga tcgcggccgg agcgaggctg aatccctgta
      961 ccgcgacctc gaattcgagc ggctggggca cggcggccga ctgcctgaac gggatctcga
     1021 acggcacgct gacggctccc ggcgcgcggt cggtgagcag gggtgcgcga accccggcat
     1081 cgaaggcctg ggcaaggctc gggacggcca atccggcaag aatgacgatc agtccgggca
     1141 gggttttcag gaggcgaagc atcgggcggg tcagcgtaca gacacattcg acgggcggca
     1201 gttgcgcgaa ccgcttcccg acctccgcta tcttggctct tcggctgccc taagccaacc
     1261 cgccgtaaac cccatgcaga tcgaaaattt cctcttctcg gtttccgatg cgctccgctg
     1321 gccggtgctc gtcgcggtgc tgctcgcatt ggcctggtcg gtgatcgaga tcggcatcct
     1381 gctggccgag gtctggcgtc gccgctggcg ttcgatcaac gccctcgaga acgcggtcga
     1441 ccaggccgag atcgacatcg tccagggtga tc
//
LOCUS       DAOTIP010000601         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_103493_length_1469_cov_2.753182, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000601 DAOTIP010000000
VERSION     DAOTIP010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103493_length_1469_cov_2.753182"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1102
                     /locus_tag="P5138_10285"
     CDS             152..1102
                     /locus_tag="P5138_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRV61008.1"
                     /translation="MAATDPKSASSIRVAVIDIGTNSTRLLIADVEGREVDEIYRESR
                     VTRLGRGVDYSGQLSAEGIEAVCRAIADYMPLIEEEEAKEVMVIATSAVRDAENGGAF
                     VAELRERFDLRCQVIDGDQEANFTYLGTSSGRDGEETMLVIDIGGGSTEMIIGRGPEP
                     IFHTSLQAGVVRHTERILTGDPPGSAELESLARDIEKLVQASLEGVDLPPIQRAIAVA
                     GTPSSLAAIDLELVPYDPKAVEGHRLPLRTVQWWLSRLASMPLAERQQVPGLHPDRAQ
                     AIVAGVVILIEIMRNFDLLEVEVSEHDILYGCAITAASGD"
     gene            complement(1142..>1469)
                     /locus_tag="P5138_10290"
     CDS             complement(1142..>1469)
                     /locus_tag="P5138_10290"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRV61009.1"
                     /translation="MLEYPDADYAGLREFGERSRDRAREAWAGRPKNISMPKIGGKGG
                     AGGEGTAAGGGTGEAETTVLPAQPPASSPEDSRLDQLEKLGRLKEAGVLDDEEFAAEK
                     KRILGG"
BASE COUNT          247 a          500 c          473 g          249 t
ORIGIN      
        1 ccgggccagt ctattgccgt ctcgctcgcg aaaaacccgg accggacgac accgccttcc
       61 agcgggaccg ggtggtcggg cggatcggca gttcatgccg gcgtgaccag tcgttcaccc
      121 cgagaatatc cccggggaat attcttgagg cttggccgcc accgatccca agagcgcctc
      181 atcgatccgg gtcgcggtta tcgacatcgg caccaattcg actcgactgc tgatcgccga
      241 cgttgaaggc cgtgaggtag acgagatcta cagggagagc cgggtcacca ggctgggccg
      301 cggggtcgac tactcgggcc agctttcggc cgagggaatc gaagcggtct gccgcgcgat
      361 cgccgactac atgccgctga tcgaggagga agaggcgaag gaagtgatgg tgatcgcgac
      421 cagcgcggtg cgggatgccg agaacggcgg tgccttcgtg gccgaactca gggagcgttt
      481 cgacctccgt tgccaggtga tcgacggaga ccaggaagcg aacttcacct acctgggaac
      541 ctcttccgga cgcgacggcg aggagacgat gctggtgatc gacatcggcg gcggctcaac
      601 ggagatgatc atcggccgcg gccccgagcc gatcttccac acctcgctcc aggcaggcgt
      661 ggtcagacac accgaacgga tcctgaccgg cgacccgccc ggttcggccg agcttgaatc
      721 gctggccaga gatatcgaga agctggtcca agcctcgctc gagggcgtgg acctgccccc
      781 cattcagcga gcaattgcgg ttgcgggcac gccgtcctcc ctggccgcga tcgaccttga
      841 actggtgccc tatgacccca aggccgtcga aggccaccgg ctgccgcttc gcaccgtcca
      901 gtggtggctc tcacgactag cctcgatgcc acttgccgaa cgccagcaag tgccgggcct
      961 ccaccccgac cgggcccagg cgatcgtcgc cggggtcgtg attctgatcg agatcatgcg
     1021 caacttcgac ctgctggagg tcgaagtgtc cgaacacgac atcctctacg gctgcgcgat
     1081 caccgccgct tcgggcgact gaagccgcgg cagcggaccg gggtccggcc ggctttccgg
     1141 ctcagccgcc gaggatccgt ttcttctcgg ccgcgaactc ctcgtcgtcg agcacgccgg
     1201 cttccttgag gcgtcccagc ttctcgagct gatcgagccg gctgtcctcg ggacttgagg
     1261 ccggcggctg ggcgggcagc accgttgttt cggcttcgcc ggtaccacct cccgcggccg
     1321 tgccttcacc accagcgcca cccttgccgc cgatcttcgg catcgagatg ttcttcggcc
     1381 gtcccgccca ggcctccctg gcccggtcgc gggatcgctc cccgaactca cgaaggccag
     1441 cgtaatcggc gtcgggatat tcgagcatg
//
LOCUS       DAOTIP010000602         1468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_103584_length_1468_cov_3.257608, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000602 DAOTIP010000000
VERSION     DAOTIP010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103584_length_1468_cov_3.257608"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="P5138_10295"
     CDS             <1..1061
                     /locus_tag="P5138_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRV61010.1"
                     /translation="FVVPFIAYIPAQDFGFSGILAVVVCGLVTSGLSARYLRAEDRLT
                     EETNWQTLAFLMEGFIFLLMGISLEDVITYAGDKGFDIDQAVLYGLLAATLLIVVRVI
                     FVVPLVAMIKRDDRRAADAKPRVEDWKEKLESEDSPERRGVQASERMERRVAAVSADI
                     DFRIKQSLGWRAGAVLAWSGMRGAVTVAAAQTLPVDTPYRAELVLIAYVGAAAMLLLQ
                     GSTLPWVIRKLNVEGDDPIEIRQQYGELIGEMSTAGNDRLEQLRATESLDSGLVDRVR
                     QDSLIRVKDPSVEQDDFDQMREEYIRLRLDVLGAERERMLHHRSVGTYSSEVIDKAQL
                     MLDLEEARLEQVSESETG"
     gene            complement(1062..>1468)
                     /locus_tag="P5138_10300"
     CDS             complement(1062..>1468)
                     /locus_tag="P5138_10300"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HRV61011.1"
                     /translation="FIHSIMYAAKESHDALPAETLRDLTDFVPPALHARAARVVTSVA
                     GRFTRPPWNVIISNVPGPPIDLYFAGARLEAMYPLSIISDGIGLNVTIMSYRDSIDIG
                     ITADREQTPEVQTLVDGMESELALLLAASKAR"
BASE COUNT          268 a          420 c          514 g          266 t
ORIGIN      
        1 ccttcgtcgt tcccttcatt gcctacatcc ccgcccagga cttcggattt tcgggaatcc
       61 tcgccgtggt cgtttgcggc ctggtcacca gtgggctgtc ggccagatac ctgcgggccg
      121 aggatcggct gacggaagaa accaactggc agacgctggc tttcctgatg gagggattca
      181 tcttcctgct gatggggata agcctcgaag acgtgatcac ctacgccggc gacaagggct
      241 tcgacatcga tcaggcggtc ctctacggtc tgctcgcggc caccctgctg atcgtcgtcc
      301 gggtgatctt cgtggtgccc ctggttgcca tgatcaaacg cgacgaccgg cgggcggctg
      361 acgccaagcc gcgggtagag gactggaaag agaagctgga aagcgaggac tccccggagc
      421 gaaggggtgt ccaggcctcc gagcgcatgg agcggagggt cgcggcggtc agtgccgaca
      481 tcgactttcg gatcaagcag tcgctcggct ggagggcggg tgccgtgctc gcctggtccg
      541 gaatgcgcgg cgcggtaacg gttgcggccg cgcaaaccct tcccgtggat acgccctacc
      601 gggcggaact cgtgctgatc gcctatgtgg gtgccgcggc gatgctgctg ctccaggggt
      661 cgaccctgcc ctgggtgatc cggaagctga acgtcgaggg cgacgacccg atcgagatca
      721 ggcagcagta cggcgagttg atcggcgaaa tgtccacggc cggcaatgac cggctggagc
      781 agctgcgggc caccgaatcc ctcgactccg gtctggtcga ccgggtcagg caggacagcc
      841 tgatccgggt caaggatccg tcagtggagc aggacgactt tgaccagatg cgcgaggaat
      901 acattcggct ccggctggac gtgctcggtg ccgagcgcga gaggatgctc catcaccggt
      961 cggtcggcac ctacagttcg gaagtcatcg acaaggcgca actgatgctg gatctcgagg
     1021 aggccaggct cgaacaggta tccgagagcg aaaccggctg atcagcgggc tttggatgcg
     1081 gcgaggagaa gcgccagctc ggattccatg ccgtcgacga gggtctggac ttccggggtc
     1141 tgctcgcggt cggcagtgat ccctatgtcg atcgaatcgc gatagctcat gatcgtgacg
     1201 ttgaggccga ttccgtcgga gatgatcgat agcgggtaca tggcttcgag ccgcgctccg
     1261 gcgaaataga ggtcgatcgg cgggccggga acgttggaga tgatcacgtt ccagggtggc
     1321 ctggtgaacc ggccggcaac cgaggtcaca acccgggcgg ccctcgcgtg gagagctggc
     1381 ggtacgaagt ccgtgagatc acgcagggtc tcggcaggaa gggcatcgtg gctttccttg
     1441 gccgcgtaca tgatcgagtg aatgaatt
//
LOCUS       DAOTIP010000603         1465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_103903_length_1465_cov_3.228369, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000603 DAOTIP010000000
VERSION     DAOTIP010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103903_length_1465_cov_3.228369"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..179
                     /locus_tag="P5138_10305"
     CDS             15..179
                     /locus_tag="P5138_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61012.1"
                     /translation="MAIGFNHEFFRDGLEAIKEDEVLLRLISPLRPGLLQPVDGDDFR
                     YLVMPIRLNE"
     gene            183..1334
                     /gene="recF"
                     /locus_tag="P5138_10310"
     CDS             183..1334
                     /gene="recF"
                     /locus_tag="P5138_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRV61013.1"
                     /translation="MFVARVEADRVRSLAGVKFEPDPGLTVIIGPNGAGKTNLVESIF
                     FGLTSRSFRTSDRRDLIPFGSSYARCRVTVSGTGVDHTFMASASRSEGNRFTMDGARI
                     EQAEAVVHRPKVTVFSPDRLELVKGPPAGRRAHIDSFVAARWPGQAGVRASFGRILAQ
                     RNALIARISAGQADTSQLSTWNSQFAEAGSALAVSREKAVGELGERWESSAGELGLEG
                     PKSLSYRPGAATTAEEIRTGLDEKLENDLRLGRSSWGPHHDEIRLELDGRQLRRFGSQ
                     GQQRLGLLALLFAERSALLEAGGQAPLMLLDDVMSELDAHRRDRLVERLLEGGQAIIT
                     AAEGELIPDREGICRVAITDLLAAAEGGQPEDSDPGSKEIGSADPGAAA"
BASE COUNT          251 a          469 c          516 g          229 t
ORIGIN      
        1 tcgacggcga ggaactggcg atcggcttca atcacgagtt cttccgtgac ggtctcgagg
       61 cgatcaagga ggacgaggtc ctgctgcggc tgatctcgcc gctccggccc ggcctgctgc
      121 agccggtgga cggcgacgac ttccgctacc tcgtcatgcc gatccgcctc aacgagtaga
      181 ccgtgttcgt cgcccgggtc gaggcggacc gggtccggtc cctggccggg gtgaaattcg
      241 agccggatcc cggcctgacc gtgatcatcg gtccgaacgg cgccggcaag accaacctgg
      301 tcgagtcgat cttcttcggc ctgacctccc gctccttccg gacctcagac cgcagggacc
      361 tgatcccttt cgggtcctct tacgcgcgtt gccgcgtcac cgtgagcggg accggggtgg
      421 accacacctt catggcttca gcctccaggt cggagggaaa caggttcacc atggacggcg
      481 cccggatcga gcaggcagaa gcggtggttc atcgtccgaa ggtgaccgtc ttctctcccg
      541 atcgcctgga gctggtcaag ggcccgccgg ccggcagaag agcccacatc gacagcttcg
      601 tcgcggcccg gtggcctggt caggccggag tgcgggcttc gttcggccgg atcctcgccc
      661 aacgaaatgc cttgatagcg cggatttcgg ccggtcaggc cgacacctcc caactatcca
      721 cgtggaattc acagttcgca gaggcgggct cggccctggc cgtgagccgc gaaaaggcag
      781 tcggggaact gggcgaacgc tgggaaagct cggccgggga actcggcctc gaaggcccga
      841 agtccctgag ctaccggccc ggggccgcga ccaccgcgga agagataagg accggtctcg
      901 acgagaagct ggaaaacgac ctaaggctgg gccgcagcag ctggggtcct caccacgacg
      961 agatccggct cgaactggac ggccggcagc tccggcgttt tggttcgcag ggacagcagc
     1021 gactcggcct cctggccctg ctgtttgcag agcggtcggc cctgctggaa gccggcgggc
     1081 aggctccgct gatgctgctt gacgacgtga tgagcgaact cgacgcccat cgtcgcgacc
     1141 ggctggtcga acgactgctc gagggaggcc aggcgatcat cacggccgcc gaaggcgagt
     1201 tgattcccga ccgggaggga atctgccggg tcgcgatcac agatcttctg gccgctgccg
     1261 aaggcgggca gcccgaggat tcggatccgg gcagcaagga gatcggctcg gcggatccgg
     1321 gagcagcggc gtgagagacg gtcgcggaag gagcccgtca ccgaagcggc tcgggcaggc
     1381 ccttgaagac ttccgcgggg agatcagccc ggcaaccccg ctggctgccg ttcagacggt
     1441 ctgggacgag gtggtggggg agagg
//
LOCUS       DAOTIP010000604         1462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_104233_length_1462_cov_3.253021, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000604 DAOTIP010000000
VERSION     DAOTIP010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104233_length_1462_cov_3.253021"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1213
                     /locus_tag="P5138_10315"
     CDS             56..1213
                     /locus_tag="P5138_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRV61014.1"
                     /translation="MSRAAQMVNRAALSGCGLLAATLLVILFAVPSPAGASSTALKSQ
                     GATASIVNGRNTTIGEWPWQVALTVSRQVAPRALTSRRFFCGGSILAPRLVITAGHCV
                     ADLNRRQIRRIEIVSGRTRLNSNRGEVARVTGLRMPVDSAGKRRYKTLLGAADWDVAL
                     LTLATPLSSEPIKLAGPDEAGTWSPGHIAWTTGWGITKAFANRVPANLQVATQVLMGK
                     GLCRRTDGIAFQATRMVCIGGPRGHTSACNGDSGGPLVVATSEGYRLVGLTSYGDGAC
                     RGFVPSVDTKVSGVPIRNWVRVTAMNLTGRDVVGSGGTAGPVRQWCKVPAIFGLKTVQ
                     AKRRLEAANCRLGRVRTDPWGAGRSGRVIGYSRLPGWLAPSGFKLNVWVSP"
     gene            1231..>1462
                     /locus_tag="P5138_10320"
     CDS             1231..>1462
                     /locus_tag="P5138_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61015.1"
                     /translation="MASKRIPGLIATILAAGALSISLAACGSDDDGGTSGTSGSDVAT
                     KSTGKDGQVTPDENAPASAKDKDPNAPDKGISL"
BASE COUNT          239 a          520 c          470 g          233 t
ORIGIN      
        1 gcaatcacac atatggcttt aggcgccctg aaggcgaccc ggtacgattc atgcaatgtc
       61 cagggctgcc cagatggtca accgggccgc cctctcggga tgcggcctcc tcgccgcgac
      121 tttgctggtg attctctttg ccgttccctc accggccggt gcctcgagca ccgccctgaa
      181 gagtcagggc gccaccgcct cgatcgtcaa cggccgcaac accacgatcg gcgaatggcc
      241 ctggcaggtt gctctgaccg tttcccgtca ggtcgccccg agggccctga ccagccggcg
      301 cttcttctgc ggtggctcga tcctggctcc gcggctggtg atcaccgccg gtcactgcgt
      361 cgctgacctg aatcggcgac agatccgccg gatcgagatc gtctccggcc gcacccgcct
      421 gaattcgaac cgcggtgaag tcgcccgtgt gaccggcctt cgcatgcccg tcgactccgc
      481 cggcaagcgc cggtacaaga cgcttctcgg cgcggccgac tgggacgtag ccctgctcac
      541 gctcgccact cccctttcga gcgagccgat caagctggcc ggcccggacg aagccggaac
      601 ctggtcgccc ggccacatcg cctggacgac cggctgggga atcacaaagg ccttcgcgaa
      661 ccgggtgccc gccaaccttc aggtcgccac gcaggtcctg atgggcaagg ggctctgccg
      721 gcggactgac ggaatcgcct tccaggcgac ccggatggtc tgcatcggcg ggcctcgcgg
      781 acacacttcc gcctgcaacg gcgacagcgg cggtcccctc gtggtcgcaa cttccgaggg
      841 ctaccggctg gtcggtctga ccagctacgg cgacggcgcc tgccggggct tcgtcccgtc
      901 ggttgacacg aaggtgtccg gagtgccgat ccgcaactgg gtccgggtca cggcgatgaa
      961 cctgaccgga agggacgtgg tcggcagcgg aggcaccgcc ggaccggtga ggcaatggtg
     1021 caaggttccc gccatcttcg gcctgaagac cgtccaggcc aaacgacggc ttgaagcggc
     1081 caactgtcgc ctcggccggg ttcgaaccga tccctggggg gccggtcgca gcggccgggt
     1141 catcggctac agccgcctgc cgggctggct ggccccgtcc ggtttcaaac tgaacgtatg
     1201 ggtctcaccc tgatcgggta tcaagaagcc atggccagca aacgcattcc cggactgatc
     1261 gccacgatcc tcgccgcagg cgccctttcg atcagcctcg ccgcctgcgg ctccgacgat
     1321 gacggcggca cctcaggcac ttcggggtcg gacgtggcca cgaagtcgac cggcaaggac
     1381 ggacaggtca ctccggacga gaatgctccg gccagcgcca aggacaagga tccgaacgcc
     1441 ccggacaagg gcatctcgct cc
//
LOCUS       DAOTIP010000605         1462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_104255_length_1462_cov_2.701493, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000605 DAOTIP010000000
VERSION     DAOTIP010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104255_length_1462_cov_2.701493"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="P5138_10325"
     CDS             complement(<1..221)
                     /locus_tag="P5138_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRV61016.1"
                     /translation="MRAIVFTTLAIAAALSLSACGFGEEGITVSKDSPDYEGAELFAA
                     NCAGCHTLTPAGALGTGNRGTRVQGPNLD"
     gene            254..754
                     /locus_tag="P5138_10330"
     CDS             254..754
                     /locus_tag="P5138_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRV61017.1"
                     /translation="MAAPDRNEFRQTVGLLPTGVTVVSAFQASGPAGATASAVCSLSL
                     EPMMMLVCLDLESRSLEAVREAGRFGISVLGESQRTAAELFATKSAQEEKWSSVAWRD
                     RLGVPIIEGSLARVVCELAEVIPGGDHVIVTGEVLDVEADDEPGEPLIYHGGLFRRLD
                     AGPEGN"
     gene            757..>1462
                     /locus_tag="P5138_10335"
     CDS             757..>1462
                     /locus_tag="P5138_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRV61018.1"
                     /translation="MPGHPFFWLDVFAEAPLSGNGLAVVLEADGLTDETMLAFARETG
                     LSETTFVQSASAEAAAAGADYRNRIWTIAEEIPFAGHPSLGTAVAVALGDGLDEASLT
                     QETRVGLQRVDVFRQDEAWLASVVQEPAEFGEVFPPERVAPALGIDPDLIDSRYSSQL
                     VSTGLPALLVPMRDLDALAACRGDADGLNTLELGLTLNLYPFVFDVPSRTVRSRCFAL
                     DLGDFEDPATGSAAGPL"
BASE COUNT          225 a          468 c          499 g          270 t
ORIGIN      
        1 tgatccagat tcggtccctg gacgcgcgtt ccacggttac ccgttcccag cgctccagcg
       61 ggggtcagtg tgtggcagcc ggcgcagtta gcggcgaaga gttcggctcc ctcgtaatcg
      121 ggggagtcct tggagaccgt gatcccctct tcaccgaatc cgcaagcgga caggctcagg
      181 gcggcggcta tggcaagtgt tgtgaagacg atggcacgca cagcgagcgc atcatattgt
      241 ctgcggcgaa acgatggctg ccccggaccg aaacgagttc cgacagacgg tcggtttgtt
      301 gccgaccgga gtcaccgtcg tctcggcctt tcaggcttcg ggaccggccg gagcgacggc
      361 ctccgcagtc tgttcccttt ccctcgaacc gatgatgatg ctggtctgcc tcgacctcga
      421 gtcgcgttcg ctcgaggcgg ttcgcgaagc gggaaggttc ggaatctcgg tgctgggtga
      481 atcccagcgg acagcagcag aactgttcgc cacgaagtcc gcccaggaag agaagtggag
      541 ttccgtggcc tggcgcgacc gtctcggagt gccgatcatc gaaggcagtc tggccagggt
      601 ggtctgcgag cttgccgagg tcatcccggg cggggaccat gtgatcgtca ccggggaggt
      661 gctcgacgtc gaagccgatg acgaacccgg tgagcctctg atctaccacg gagggctctt
      721 ccgccggctc gacgcggggc ccgaggggaa ctgaacgtgc ccgggcatcc gtttttctgg
      781 cttgacgtgt ttgccgaggc gccgctgtcc ggcaacggtc tggcggtcgt actcgaggcg
      841 gacgggttga cggacgagac gatgctcgcc ttcgcccgcg agaccggatt gtcggaaacc
      901 accttcgtcc agtccgcctc ggccgaagcg gctgcggccg gtgccgacta caggaaccgg
      961 atctggacca tcgcggagga aattcccttc gccgggcatc cctcgctggg aactgccgtg
     1021 gcggtggcgc ttggcgacgg gctggacgag gcatcgctta cccaggaaac ccgggtcggc
     1081 cttcaacggg tggacgtttt ccggcaggac gaagcctggc ttgcatcggt cgtccaggag
     1141 ccggccgaat tcggcgaggt gttcccgccg gagcgggtgg ccccggccct cggcatcgat
     1201 ccggacctga tcgacagccg ctactcatcc cagctggtct cgaccgggct gccggctctg
     1261 ctcgtcccga tgcgggacct cgacgcgctg gcggcctgcc gtggcgacgc ggacggcctg
     1321 aacaccctcg agctcggtct gaccctgaat ctctatccgt tcgtgttcga cgtcccgagc
     1381 cggacggtgc gttcccgttg cttcgctctc gacctcggtg atttcgagga ccccgcaacc
     1441 ggatcggctg ccggcccact gg
//
LOCUS       DAOTIP010000606         1461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_104335_length_1461_cov_3.172119, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000606 DAOTIP010000000
VERSION     DAOTIP010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104335_length_1461_cov_3.172119"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="P5138_10340"
     CDS             complement(<1..328)
                     /locus_tag="P5138_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRV61019.1"
                     /translation="MKSNGKRKAGQRVFVALDPPSAVREEAEAWARQLARGTHGLRVV
                     PARNSHITLAFMAQRDEYEIESIIDAMTRSAGPVGGLGLGAPVWLPRRRPRNLVLDIH
                     DDRGQLA"
     gene            complement(440..>1461)
                     /locus_tag="P5138_10345"
     CDS             complement(440..>1461)
                     /locus_tag="P5138_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61020.1"
                     /translation="RNREKLEALADSLGDHGPKPEVHAVPNTDSRGMKELFSRSAAVI
                     ACAGPFSLHGEPVLAAAVEAGVPYLDTTGEQDFIRRAFEVYGPRATKAGCPVLPAMGF
                     DYVPGDMLAALTAAGLDEGESVDKVRLAYHAPFQPSRGTMRSAIEMIKGGDVEWRERA
                     LRPASQAVARPDFDFGGRLGVQKMMHYPAAEHITVPTHLAPRTVETGLTVSSFAPGTA
                     GQVLLPPINRALGLAMRTPLRKAMSGAISAMPEGADEKSRTASEFTVACDVTSARMNV
                     RGTLTGTDVYGLTAALITEGAIRCARGEVGGNGALAPATAFEPAGFLAGFGRFKLDWE
                     ITAAR"
BASE COUNT          226 a          502 c          502 g          231 t
ORIGIN      
        1 gggccagctg cccccgatcg tcgtggatgt cgagaaccag gttgcggggc cggcgccggg
       61 gcagccacac gggggcgccg aggccgagac caccgaccgg cccggcggaa cgggtcatcg
      121 cgtcgatgat gctttcgatt tcgtattcgt cgcgctgagc cataaaggcc agggtgatgt
      181 gcgaattgcg ggccggcacc accctcaggc catgcgtgcc ccgggccagt tgccgggccc
      241 acgcttccgc ttcctcgcgg accgccgagg gagggtccag cgccacgaac acccgctggc
      301 cggccttacg cttcccgttg cttttcattc ggggctccct gactgcttgg gggaatgatc
      361 tgttccgagc gtactccgta atccagcgga accgggacac cggtcggcct ctgcccgacg
      421 atcccgaacg gagcctcggc tagcgggctg ccgtgatctc ccaatcgagc ttgaaacgtc
      481 cgaaaccggc gaggaagccg gccggctcga aggcggtcgc cggggccagg gcgccgttac
      541 cgccgacctc accccttgcg cagcggatcg caccttcggt gatcagcgcg gcagtcagtc
      601 cgtagacatc ggtcccggtc agggttccgc gaacattcat ccgggcggag gtcacgtcac
      661 aggcgaccgt gaactccgat gcggtcctcg acttctcgtc ggcgccttcc ggcatggccg
      721 agatcgcacc tgacatggcc ttgcgcagcg gggtcctcat cgcgaggccc agggcgcgat
      781 ttatcggcgg cagcaggacc tggccggcgg tgccgggggc gaaggaggaa acggtcaggc
      841 cggtctccac cgtgcggggc gcgagatggg tgggaaccgt gatgtgctcg gcagcggggt
      901 aatgcatcat cttctggact cccagccggc cgccgaagtc gaagtccggc cgggcgaccg
      961 cctgcgacgc ggggcgaagc gcccgctccc gccattcaac gtcacccccc ttgatcattt
     1021 cgatcgccga acgcatggtt ccccggctcg gctggaaagg ggcgtggtag gcgaggcgga
     1081 ccttgtcgac gctctcgcct tcatccagtc cggcggcggt caacgcggcc agcatgtcgc
     1141 cgggcacata gtcgaaaccc atcgccggga gcaccgggca gccggccttg gtcgctcggg
     1201 ggccgtacac ctcgaaggcc cgccggatga agtcctgctc gccggtcgtg tccagatagg
     1261 gaacgccggc ctcgactgcc gcggcaagca ccggctcgcc atggagagag aacggcccgg
     1321 cgcaggcgat caccgccgca ctccgcgaga agagctcctt cataccgcgg gagtcggtgt
     1381 tcggcaccgc gtggacttcc ggcttggggc catggtctcc gagactgtcg gccagagcct
     1441 cgagcttctc ccggttccgc c
//
LOCUS       DAOTIP010000607         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_104989_length_1455_cov_3.271429, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000607 DAOTIP010000000
VERSION     DAOTIP010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104989_length_1455_cov_3.271429"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5138_10350"
     CDS             <1..200
                     /locus_tag="P5138_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV61021.1"
                     /translation="RRDRAMTTSVLDGIRGVGPGRKRLLIRHFGSPDRLLEASREELE
                     AVPGLPGKVAREIHEQLNRIG"
     gene            206..1102
                     /gene="rapZ"
                     /locus_tag="P5138_10355"
     CDS             206..1102
                     /gene="rapZ"
                     /locus_tag="P5138_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRV61022.1"
                     /translation="MSVPDTGTDPQPDPVEMVVITGLSGAGKSEAIAAFEDAGYFCVD
                     NLPPQMIGALGDLFRLADSGVEKAAVVSDARGGEYFDQLNQVLDDLEGDEIKPKLVFL
                     EAHDEILTDRYKETRRRHPSAPEGRIAEGIEREREILAPLRARADVVIDTSGETAATL
                     RRRISEELIGTASGGLMVVNLLTFGFKNGPPRDADITLDVRFLPNPHYVDELRPLTGL
                     DQEVRDYVEAGTTAGEFYGRLLPLLEFLIPAYVTEGKSHLTIAIGCTGGRHRSVTVAE
                     RIWRDFSTRSDVTVRVTHRDIS"
     gene            1122..>1455
                     /locus_tag="P5138_10360"
     CDS             1122..>1455
                     /locus_tag="P5138_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV61023.1"
                     /translation="MSEPVISIRGLRKSYDELEAVRGIDLEVFPEEIFALLGPNGAGK
                     TTTVEILEGYRDRDAGEVSVLGVDPASPTAAWRERIGIVLQSTTLEPVLTVRESLQQY
                     AGYYRNPRD"
BASE COUNT          270 a          475 c          482 g          228 t
ORIGIN      
        1 gacgccgcga ccgcgccatg acgacctcgg tcctggatgg catacgcggg gtgggccccg
       61 gccggaagcg gctcctgatc aggcatttcg gctcacccga ccgcctgctg gaggccagcc
      121 gggaagagct ggaagcggtg cccggactgc ccggtaaggt ggcccgtgaa attcacgagc
      181 aactgaacag gatcggatag ttccaatgtc agtcccggac acagggacgg accctcagcc
      241 cgacccggtc gagatggtgg tgatcaccgg gctctccggg gccggaaagt cggaagcgat
      301 cgccgccttc gaagatgccg gctatttctg cgtggacaac cttcccccgc agatgatcgg
      361 cgctctgggt gacctgttcc gcctcgccga cagcggagtc gaaaaggcgg ccgtggtctc
      421 ggacgcccgc ggcggcgagt acttcgacca gctcaaccag gtcctcgacg accttgaagg
      481 cgacgaaatc aagcccaaac tcgtcttcct cgaggcgcat gacgaaatcc tcaccgaccg
      541 ctacaaggag acccggcgac gtcacccctc ggcaccggag ggaaggatcg ccgaggggat
      601 cgaacgcgaa cgcgagatcc tcgccccgct ccgggcgcgg gccgacgtgg tgatcgacac
      661 ttccggagaa accgcggcga cgctcaggcg gcggatctcg gaagagctga tcgggacggc
      721 atcaggcggg ctgatggtgg tgaatttgct caccttcggc ttcaagaacg ggccgccccg
      781 ggacgccgac atcaccctcg acgtccgttt cctgccgaat ccgcattacg tcgatgagct
      841 caggcccctg accgggctgg accaggaagt cagggattac gtggaagcgg gcaccacggc
      901 cggagagttc tacggacgcc tgctgcccct gctcgagttc cttatccccg cctatgtgac
      961 ggaagggaag agccacctga ccatcgccat cggctgtacc ggcggccggc accggtcggt
     1021 gaccgtggcc gagaggatct ggcgggactt ctcgacccga agtgacgtga cggtcagggt
     1081 cacccaccgc gatatctcct gagtatctag ggttgttggc cgtgtcagag ccggtcatat
     1141 ccatccgggg cctgcgcaag tcttacgacg agctcgaggc ggtcaggggc atagacctcg
     1201 aggtctttcc agaggagatc tttgccttgc tgggcccgaa cggggccggc aagacgacca
     1261 cggtcgagat tctcgagggc taccgggacc gcgacgccgg cgaggtcagc gtgctgggag
     1321 tggatcccgc cagtccgacc gccgcctggc gcgagcggat cggcatcgtt ctccagagca
     1381 cgaccctgga gccggttctc accgtgcgcg aatcgctcca gcagtacgcc ggctactacc
     1441 gcaacccccg ggacg
//
LOCUS       DAOTIP010000608         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105013_length_1455_cov_2.727143, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000608 DAOTIP010000000
VERSION     DAOTIP010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105013_length_1455_cov_2.727143"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="P5138_10365"
     CDS             complement(<1..654)
                     /locus_tag="P5138_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61024.1"
                     /translation="MGITNARRYAAIAFALIMAFALMASFASSQAEAGKKKKAKPVKV
                     TVAGIPTANQQQLLNQKKLVVKVKSTGKATVNLSAIGAGKNGTFKSKKVKFKKKGVKK
                     ISLALTSKGKSVLGTCGAKTVKVNGNYKRKVKKGKKTKNKTVKFSKKKQLAKWAGNDD
                     CIDYVSVDLGDDPANCDFLDATQCLQPWPNDYYTKEDSSTPTGKRLNLGADSTPMNTG
                     "
     gene            complement(888..>1455)
                     /locus_tag="P5138_10370"
     CDS             complement(888..>1455)
                     /locus_tag="P5138_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV61025.1"
                     /translation="IAGGRLVSRSMDNRLGSYVALEAARRIHENSTAKVRFAGVAAVQ
                     EEIGLFGSRTAAFTVRPDLAVAIDVTHATDAPGIDEKEVGSHPFGSGPVIGRGSTLSP
                     RVYELLRETAEKAGIDYSVGASGNSTHTDADSLQISRTGIPTGLVSIPLRYMHSPVEM
                     VDLADVEATVELMVAFAESLDPGVDLSR"
BASE COUNT          236 a          441 c          462 g          316 t
ORIGIN      
        1 gcccgtgttc atcggggtcg aatccgcacc gaggttcagg cgcttgccgg tcggcgtcga
       61 cgagtcttcc ttggtgtagt agtcgttcgg ccagggctgg aggcactggg tcgcatcaag
      121 gaagtcgcag ttcgcgggat cgtcaccaag gtctacggac acgtaatcga tgcagtcatc
      181 gttacccgcc cacttggcaa gctgcttctt cttcgagaac ttgaccgtct tgttcttggt
      241 cttcttgccc ttcttgacct tgcgcttgta gttgccgttg accttgacgg tcttggcccc
      301 acaggtgccg agcacggact tgcccttgct ggtcagggcg agggagatct tcttgacgcc
      361 cttcttcttg aacttgacct tctttgactt gaaggtgccg ttcttgccgg cgccgatggc
      421 cgaaagattg acggtggcct tgccggtcga cttcaccttc accacgagct tcttctggtt
      481 cagaagctgc tgctggttgg cggtcgggat accggcgacg gttaccttca ccggcttggc
      541 cttcttcttc ttacctgcct cggcctggct gctggcaaac gatgccatca gcgcgaaggc
      601 catgatcagg gcgaaagcaa tcgccgcata acggcgcgcg ttcgtgattc ccacttggtg
      661 atccatgtct ccgtgctact ccctctgttg ggcgccggtc ggaaagtccg gctcagtttt
      721 tcggaccggt ccagacgaga ccgatcgtgt gacttgtgtc acagatacta gaccggatta
      781 tggctggaag ttgcgccgaa tggacacagt gtttttgaac ggttgcgtac cgaaatcgaa
      841 cggctgcgta cctcaatgac ccttgcgcgg ggccgggcga cggctcctca gcgcgacagg
      901 tcgaccccgg ggtcgagcga ttcggcgaag gcgaccatca actccaccgt ggcctccacg
      961 tcggccaggt cgaccatctc gaccggggaa tgcatgtagc ggagcgggat cgatacgagt
     1021 ccggtcggga tgccggtccg tgagatctgc agggagtcag cgtctgtatg ggtcgagttg
     1081 ccgctggccc cgaccgagta gtcgatgccg gccttttcgg ccgtttcgcg gagcagctca
     1141 tagaccctgg gcgaaagtgt ggacccgcgg ccgatcaccg gaccagaccc gaaggggtgg
     1201 ctcccgactt ccttctcgtc gatcccgggg gcgtcggttg cgtgggtgac gtcgatcgcc
     1261 actgccaggt cgggccggac ggtgaaggcc gcggtccggg atccgaacag cccgatctcc
     1321 tcctggacgg cggcgacgcc ggcgaaccgt accttcgccg tcgagttctc atggatgcgg
     1381 cgggcagcct ccagggccac ataggaaccg agccggttgt ccatcgagcg cgaaaccaga
     1441 cggccgccgg caatc
//
LOCUS       DAOTIP010000609         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105170_length_1453_cov_3.585122, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000609 DAOTIP010000000
VERSION     DAOTIP010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105170_length_1453_cov_3.585122"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..339)
                     /locus_tag="P5138_10375"
     CDS             complement(4..339)
                     /locus_tag="P5138_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61026.1"
                     /translation="MNRYSGLPNRKTSLTGLTDEGDEIWIIRSISQKFYNCLGCRGPI
                     EIGDEHVVVQYVRKFGGTEHSHWHQRCAEEILYSQVRGMRQVSAKESSRDRLEGRGRR
                     PAGRRRRPR"
     gene            399..1238
                     /locus_tag="P5138_10380"
     CDS             399..1238
                     /locus_tag="P5138_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRV61027.1"
                     /translation="MSPVTAETKTLSTYIQDRIDEFSRSQKDVARYIVDHLDEAAFLT
                     AEELARRAGTSSSTVVRFSQALGFEGYPELQASAIEEYRATVGGEESGGRMLFSFDHS
                     EFEASLGTDHSNLEETARKLDQTQIDEAVAALSGASQVVIVGTDQMAFFASYLRHTLS
                     LLDIRAEIVTSTAGPSLQRLGRVDEETLLITLSSGRSHALLARAMKLAGHRGARTLAI
                     ADATLSDAVKKADMSLYFSSNSPSFLRSNTALMALVHALAYGVYSLSEDEHSDRIRAY
                     KLK"
     gene            1294..>1453
                     /locus_tag="P5138_10385"
     CDS             1294..>1453
                     /locus_tag="P5138_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRV61028.1"
                     /translation="MAEEATFRVKTGLAEMLKGGVIMDVVDADQAKIAEDAGACAVMA
                     LERVPADIR"
BASE COUNT          269 a          481 c          443 g          260 t
ORIGIN      
        1 gaatcagcgg ggccgtcgcc tgcgcccggc aggacggcgg ccccggcctt cgagccggtc
       61 ccggctcgat tccttggcag agacctgacg catcccgcga acctgtgagt agaggatctc
      121 ctcggcgcag cgttgatgcc agtgagaatg ctcggtgccg ccgaactttc tgacgtactg
      181 gacaacgacg tgctcgtcgc cgatctcgat cggcccgcgg cagccaaggc agttgtagaa
      241 cttctgcgag atcgagcgga tgatccagat ctcgtcgcct tcgtcggtca ggccggtaag
      301 cgaggtcttg cgattcggaa gtcctgagta tctgttcaaa atccctgctc agagcgaatt
      361 tagaagggaa atggtgggaa ggggcgaaat gatccggcgt gagcccagtg accgccgaaa
      421 ccaagaccct gtcaacttac atccaggatc ggatcgacga gttttcccgc tcccagaagg
      481 atgtcgcgcg ctacatcgtc gaccatctcg atgaggcggc attcctgacc gcggaggaac
      541 tggcccgccg ggccgggact tcctcatcaa cggtggtccg cttctcccag gccctcggat
      601 tcgagggtta cccggaactc caggcttcgg ccatcgagga gtaccgggcc acggtaggtg
      661 gcgaagaaag cggcgggcgc atgcttttct ccttcgacca ctccgagttc gaggcctcgc
      721 tcggcaccga ccattcgaac ctcgaggaaa ctgcccgaaa gctggaccag acccagatcg
      781 acgaggccgt cgccgcgctt tccggcgcca gccaggtcgt gattgtcggc accgaccaga
      841 tggccttctt cgccagctac ctgcggcaca cgctcagtct gctcgacatc cgggccgaaa
      901 tcgtgacctc taccgccggc ccctcgctgc agcgcctcgg ccgggtcgac gaggagaccc
      961 tgctgatcac cctttcctcc ggtcgctcgc atgcgctgct ggcccgggcc atgaaactgg
     1021 ccggccatcg cggtgcccgc accctggcga tcgccgatgc gaccctgtca gacgccgtca
     1081 agaaggccga catgagcctc tacttctcgt cgaacagccc ctcgttcctt cgctcgaaca
     1141 ccgccctgat ggcactcgtc catgcgctcg cctacggcgt ctattcgctc agcgaagacg
     1201 agcacagcga ccgcatccgt gcctacaagc tgaagtagcc cgcggcccct tcgagggcgc
     1261 atcaaagggt cctccctctc tatatttggg gggatggctg aagaagcgac tttccgagta
     1321 aagacgggcc tggcagagat gctgaagggc ggcgtgatca tggacgtggt cgacgccgac
     1381 caggcgaaaa tcgccgagga tgccggtgcc tgcgcggtca tggccctgga acgcgttccg
     1441 gccgacatcc gca
//
LOCUS       DAOTIP010000610         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105190_length_1453_cov_2.879113, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000610 DAOTIP010000000
VERSION     DAOTIP010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105190_length_1453_cov_2.879113"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="P5138_10390"
     CDS             complement(<1..687)
                     /locus_tag="P5138_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRV61029.1"
                     /translation="MSTTIAILSQKGGTGKTTLTRSLSEGLRRAGLSVLAIDADPQGN
                     LSEYFGIPADAFPTLGEVLAGRERAVDAIHTGPGGGEVIPANLGLAEAELMLAGKIGR
                     ELTLRNALREPKRRFDVILIDCPPTLGLLTVNALVASDKAILSTQAEHFSEQGAEQAM
                     EVIGLARENLNPELELLGLLLNIADMRTNHARSTLETMREQFGDLVFDTVIRRSIVYP
                     ESARAAKPIFD"
     gene            747..1256
                     /locus_tag="P5138_10395"
     CDS             747..1256
                     /locus_tag="P5138_10395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRV61030.1"
                     /translation="MGPVFTEVDIDAPREEIFDYLLDFDTRPFLYGDSVENFRLLRLD
                     SRGIGAGARWQFKRKKAWADSAITATEAPRRISERGSTGSYNRNVTGTEWEMEEISTG
                     VTRVRITFWIVPAGFSRVHNRLTGGAGWHRRRLDKVGKRLREAIESARREKVEVPVAG
                     GNRHATGVA"
BASE COUNT          251 a          467 c          490 g          245 t
ORIGIN      
        1 atcgaagatc ggcttggctg cccgggctga ttcgggatag acgatcgaac gccggattac
       61 cgtgtcaaag acgaggtccc cgaattgctc gcgcatggtc tccagggtgc tgcgggcgtg
      121 attggtccgc atgtcggcga tgttgagcag cagcccgagc aactcgagct ccggattcag
      181 gttctcccgg gccaggccga tcacttccat cgcctgctcg gccccctgct cggagaaatg
      241 ctcggcctgg gtcgagagga tcgccttgtc ggacgcgacc agggcgttca cggtgagcag
      301 gcccagggtc ggcgggcagt cgatcaggat cacgtcaaaa cgccgcttcg gctcccgcag
      361 ggcgttacgg agggtgagct cgcgcccgat cttgccggcc agcatcagct cggcctcggc
      421 taggccgagg ttggcgggga tcacctcgcc gccgccgggg ccggtgtgaa tcgcatcgac
      481 cgcccgttcc cggccggcca gcacttcacc gagggtgggg aaggcatcgg ccgggatccc
      541 gaagtactcg gaaaggtttc cctgggggtc ggcatcgatc gcgagaacgg aaagtcccgc
      601 ccggcgcagg ccctcggaga gcgaccgcgt caacgtggtc ttcccggttc ctcccttctg
      661 ggaaaggatc gcgatcgtcg tggacaccgg cacaccctat cgccggtcgg gcacgcatcg
      721 gcacgcaggt cgtagaatcc ggatcgatgg gtcccgtatt cactgaagtc gacatcgacg
      781 cgccgcgcga agagatcttc gactacctcc tggacttcga cacccgcccg tttctttacg
      841 gcgactcggt cgagaacttt cggctcctga ggctggattc gcggggcatc ggcgctggcg
      901 cccgctggca gttcaagcgc aagaaggcct gggccgattc ggcgatcacc gcgaccgaag
      961 ccccccggcg gatttccgaa cgcgggtcca ccggcagcta caaccgcaac gtgaccggca
     1021 ccgagtggga gatggaagag atctcaaccg gggtaacccg ggtgcggatc accttctgga
     1081 tcgtgccggc cggattctcc cgggttcaca accgcctgac cggcggcgcg ggctggcaca
     1141 ggcgccgtct ggacaaggtc ggcaagcgtc ttcgggaggc gattgagtcg gctcgcaggg
     1201 agaaggtcga ggttccggtc gccggcggca accgacacgc gaccggtgtc gcctaggaac
     1261 cgctcagcac gtccggataa ggccgaataa actcccgcac cgatggaaag actttcgaag
     1321 agcaacctgt ttcgtggcct cgtcctggcc ctcgtagtcg ctatctccgg gctcgcgctc
     1381 gccgcttgcg gtgccagcca tgaggagaag gagggtctcg ccgaaggtga aatcgtcgag
     1441 ctcggcccgc tgc
//
LOCUS       DAOTIP010000611         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105526_length_1450_cov_2.027240, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000611 DAOTIP010000000
VERSION     DAOTIP010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105526_length_1450_cov_2.027240"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5138_10400"
     CDS             complement(<1..265)
                     /locus_tag="P5138_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRV61031.1"
                     /translation="MANRHTWPMPSPRKLCLVTGATGYIGGRLVPELLAAGYRVRVLS
                     RHPDRLRDVAWFDEVEVVRGDAGEPDQVREALRGVDVAYYLLHS"
     gene            388..768
                     /locus_tag="P5138_10405"
     CDS             388..768
                     /locus_tag="P5138_10405"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRV61032.1"
                     /translation="MIQRRTDPRVRIRSRRSPSAPAEPVPVTGPDAEAADQSPVHVEV
                     YGERPPARAMLGYIKPTLWVIAAVYVVAFLFLNSDSAQINFVFFRSEVPLFVALLVML
                     ALGMGLGALTVWLRGRHRPEKKSK"
     gene            779..>1450
                     /locus_tag="P5138_10410"
     CDS             779..>1450
                     /locus_tag="P5138_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRV61033.1"
                     /translation="MPEIHDLTALEQGRAIARGELSSVEITRHYLSRSERLDAEVGAF
                     VRLLPELALEGAAAADSAISAGQRPPDQPLFGVVCPVKDLDLVAGVPTSFGNPALEVT
                     LDVDSNVVTALREAGLVITGKTSTPEIGLPCYTEPDRLPTARNPWDLDRSAAGSSGGA
                     AAAVAAGLAPLAQGSDGGGSIRLPAAVCGLVGIKPSRGRVSSGPFSYGVGDLATSGPL
                     ARTVAD"
BASE COUNT          220 a          506 c          503 g          221 t
ORIGIN      
        1 gcgagtgcag caggtagtag gccacgtcga cgccgcgcaa ggcctcccgc acctgatccg
       61 gttcgccggc atccccgcgg accacctcga cctcgtcgaa ccaggcgacg tcgcggaggc
      121 ggtccggatg ccgcgacagc acccggacgc gatagccggc ggcgaggagt tccggcacga
      181 gccgaccacc gatgtagccg gtcgcgccgg tgaccaggca gagttttctc ggagacggca
      241 tgggccaagt gtggcggttg gccactggag ccgcacctcg gatcgcaggc actacccgaa
      301 ggggcgccca catcgcggcg cgaccgaccc ccgagcagcc gaagaggacc cctggccgtg
      361 ccttcgcggg atagcctgga gccatgaatg atccagcgca ggaccgatcc gagggtcagg
      421 atccgaagcc gtcgcagccc ttccgcgccg gccgagccgg tcccggtgac cggtccggat
      481 gcggaggcgg cggatcaatc gcccgtccac gtcgaggtct acggcgagcg cccccccgca
      541 cgggccatgc tcggctacat caaaccgacc ctgtgggtga tcgctgcggt gtacgtcgtg
      601 gcctttctgt tcctcaactc ggactccgcg cagatcaact tcgtgttctt ccgatccgag
      661 gtgccacttt tcgtggccct gctggtgatg ctggcccttg gcatggggct cggcgctctg
      721 acggtttggc tgcgcggccg gcaccgtccg gagaagaagt cgaagtagcc gtcgacgcat
      781 gccagagatt cacgatctga cggcgctgga gcaggggcgg gcgatcgcac gaggcgagct
      841 ctcatcggtg gagatcactc gccactacct ctcccggagt gaacggctcg acgcggaggt
      901 cggtgccttc gtacggctgc taccggaact ggcactagaa ggggcggccg cggcggactc
      961 ggcgatctcg gccgggcagc gaccgccgga tcaaccgctg ttcggcgtcg tgtgcccggt
     1021 gaaggatctc gacctggtgg ccggggtgcc gacgagtttc ggcaatcccg cgctggaggt
     1081 caccctcgac gtcgattcca acgtcgtcac cgcgctgcgc gaggctggtc tggtgatcac
     1141 cggcaagacc agcacgccgg aaatcggcct tccgtgctat accgaacccg atcgcctccc
     1201 gaccgcccgc aacccctggg acctcgaccg atccgccgcc ggatcgtcgg gcggggccgc
     1261 cgccgcggtc gccgccggcc tggcgccgct ggcccagggg agcgacggtg gcggttccat
     1321 ccggctgcct gccgcggtct gcggcctggt cggcatcaaa ccctcccgcg gtcgggtcag
     1381 ttccggaccg ttcagctacg gagtgggaga cctggcgacg tcgggcccgc tcgcccgaac
     1441 cgtggcggat
//
LOCUS       DAOTIP010000612         1449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105599_length_1449_cov_2.487805, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000612 DAOTIP010000000
VERSION     DAOTIP010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105599_length_1449_cov_2.487805"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="P5138_10415"
     CDS             <1..615
                     /locus_tag="P5138_10415"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRV61034.1"
                     /translation="AGVDNILALRGDPPRGQTDFVQPEGGFGSAAELAAHISSKYDFS
                     IGGACFPEVHPEAPDLDTDLTYLKTKVDAGAEFLITQLFFDNRAYFDFVDAARAKGID
                     VPILAGIIPVTGYAHTKRICSLCDASIPSALETAMLAAEGDPEAEFNLGVAYAAQQSA
                     ELLAAGAPGIHFYALNKAPATRAVLGALRAAQPWRGARGQSLPS"
     gene            complement(690..>1449)
                     /locus_tag="P5138_10420"
     CDS             complement(690..>1449)
                     /locus_tag="P5138_10420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV61035.1"
                     /translation="LAISDGPLLRLDFPIDPASIDFSATGLVTVFLRHPLGGDPPLAE
                     ATARNVCLLSDPALPRKSRASDRQQIRKNLKRGCTVEITPGQETSPEQRAGFFTAYTE
                     TMSRTGASDRYFFSESYFDLILSAENTWLFILRTPEGEAAAASIAALSDGLLHYYLSG
                     TADAHLKGAPMKNVVEEMIAFGAERELAVNFGGGISPGDALEEFKRGFANREEQWFTS
                     EIVCDPAAAAELTKALVTSQGRDAEPGFFPPYRA"
BASE COUNT          231 a          486 c          489 g          243 t
ORIGIN      
        1 gccggtgtgg acaacatcct ggccctgcgc ggcgacccgc cccgcggtca gactgacttc
       61 gtccagcccg agggcggctt cggcagcgcc gccgaactgg ccgctcacat cagctccaag
      121 tacgacttct cgatcggcgg agcctgtttc cccgaggtcc atcccgaagc cccggatctc
      181 gacaccgact tgacctacct caagacgaag gtcgatgccg gcgccgagtt cctgatcacc
      241 cagctcttct ttgacaaccg ggcctacttc gatttcgtcg atgcggccag ggccaagggc
      301 atcgacgtgc cgatcctcgc cgggatcatc ccggtcaccg gatacgccca cacgaaacga
      361 atctgcagcc tctgtgatgc gtcgatcccg tccgcgctcg agaccgcgat gctcgccgcc
      421 gaaggggacc ccgaggccga gttcaatctc ggtgtcgcct acgccgccca gcagagcgcc
      481 gagctgcttg cggccggggc gcccggcatc cacttctacg cgctcaacaa ggcgcccgcc
      541 acccgggcgg tccttggagc gctacgggcc gcccagccct ggcgcggcgc ccgcggccag
      601 tcccttccca gttagctacc gcggcagcct ggccgggttt tccgcggcct tgccggaaga
      661 tccggaacag cttgccggat tgatcccgat caggcccggt agggcgggaa gaagccgggt
      721 tccgcgtccc ggccctgact ggtcacgagc gctttggtca gttccgcggc ggcggccggg
      781 tcgcagacga tctccgaggt gaaccattgc tcctcgcggt tggcgaagcc ccgcttgaat
      841 tcttcgagag catcaccggg actgatgccg ccgccgaagt tcacggccag ttcccgctcc
      901 gcgccgaagg cgatcatctc ctcgaccacg ttcttcatcg gggcgccctt cagatgggcg
      961 tcggcggtgc cggaaaggta gtagtggagc aggccgtcgg aaagggcggc gatcgaggcg
     1021 gccgcggctt caccctccgg cgttctgagg atgaagagcc atgtgttctc ggcggagagg
     1081 atcaggtcga agtagctctc gctgaagaag taacgatcgg aggccccggt cctcgacatc
     1141 gtctcggtgt aggcggtgaa gaacccggcc cgttgttcgg ggctggtctc ctgtccgggg
     1201 gtgatctcga ccgtgcagcc gcgtttcagg ttcttgcgga tctgctggcg gtcggaggcc
     1261 cggctcttgc ggggcagggc cgggtcggag agcaggcaga cgttgcgcgc ggtcgcttcc
     1321 gccagcggcg ggtcccctcc gagtggatgc cggaggaaga cagtgaccag cccggtggca
     1381 gaaaagtcga tcgaggccgg gtcgatcgga aagtcgagac ggagcagggg gccatccgag
     1441 atcgccagg
//
LOCUS       DAOTIP010000613         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105818_length_1447_cov_2.329023, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000613 DAOTIP010000000
VERSION     DAOTIP010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105818_length_1447_cov_2.329023"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="P5138_10425"
     CDS             complement(<1..247)
                     /locus_tag="P5138_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV61036.1"
                     /translation="MSLSPHVASLTSARALIDGESGSLRAVTAMDLPILGGISIKRLL
                     LSPGSIREPHWHANAHELTYCLRGDLLVSVLDDADGFS"
     gene            324..689
                     /locus_tag="P5138_10430"
     CDS             324..689
                     /locus_tag="P5138_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRV61037.1"
                     /translation="MSEDPSLMIDQRIAELDDWRGETLERIRELIIEAVPDVVEEWKW
                     RNPVWSKDGIICTGEAYKKVVKTTFAKGASLEDPEGLFNSSLGGNTRRAIDFSEGEEI
                     DEEAFKDLIRAAAELNGDA"
     gene            complement(738..>1447)
                     /locus_tag="P5138_10435"
     CDS             complement(738..>1447)
                     /locus_tag="P5138_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61038.1"
                     /translation="QIVLCTGIYTYDYLPQFLLNRDADYIASLFVHDIEQGIQDTGVK
                     AAFIKCAADEPGVTPNIEKIHRAAARASVQTGAPIMAHSRPASGTGPKQVEIFLEEGV
                     PAERIQIAHTGDTDDLDYIESLLAQGVYIGMDRYGLDLFLPTAQRNATVTELLNRGYA
                     ERMFLSQDFDIPIANGLDWYPPEILEQLQAAGAATDWSMTFIFEQVIPALKEAGMTDD
                     QLDTMLVENPKRWLTAP"
BASE COUNT          275 a          402 c          514 g          256 t
ORIGIN      
        1 aggagaatcc gtcggcgtca tcgagcaccg agaccagcag atcccctcgc aggcagtaag
       61 tcagctcatg cgcgttcgca tgccagtggg gctcgcggat cgaaccgggg gagagcagca
      121 gccgcttgat cgagatccct ccgaggatgg ggagatccat tgcagtgacc gcccgcaggg
      181 accccgactc accgtcaatc agtgctctgg cgctggtcag ggaggcgacg tgggggctca
      241 agctcatgca ggagcgaccc tagcagcgaa gcggaggccg aaggcaacgg caaatcacgg
      301 gattccggat agagtcgcaa gcgatgagcg aagacccttc actgatgatc gaccagcgaa
      361 tcgccgagct cgacgactgg cgcggcgaga cccttgaacg gattcgggag ctgatcatcg
      421 aggcggtgcc cgacgtggtc gaggagtgga agtggcggaa tccggtctgg tcgaaggacg
      481 ggatcatctg caccggcgag gcctacaaga aggtcgtcaa gaccaccttc gccaagggag
      541 ccagtctcga agacccggag gggctgttca actctagcct cggcggtaac accaggcggg
      601 cgatcgactt cagcgaggga gaagagatcg acgaggaagc cttcaaggac ctgattcgcg
      661 ccgccgctga actcaacggg gacgcctagc ggcccgatcg gcaccttcgg ccggctccgt
      721 acccggagag gcccggctca gggagcggtc agccagcgct tcggattttc gaccagcatc
      781 gtgtcgagtt gatcgtcggt catgccggct tccttgagcg ccgggatgac ctgctcgaag
      841 atgaaggtca tgctccagtc cgtggcggcg ccggcggcct ggagttgctc gagtatttcc
      901 ggcgggtacc agtcgaggcc attggcgatc gggatgtcga aatcctggga gaggaacatg
      961 cgttccgcgt agccgcgatt cagcagttcg gtgacggtcg cgttgcgctg ggcggtgggg
     1021 aggaagagat cgagtccgta gcggtccatg ccgatgtaga cgccctgtgc gagcaggctc
     1081 tcgatgtaat cgaggtcgtc ggtgtcgccg gtgtgggcga tctggatccg ctcggccggg
     1141 acgccttctt caaggaagat ctcgacctgc ttcggaccgg ttcccgaggc ggggcggctg
     1201 tgggccatga tcggcgcacc ggtctggacg cttgccctgg cggctgcgcg gtggatcttc
     1261 tcgatgttgg gtgtgacgcc cggctcgtcg gcggcgcatt tgatgaatgc ggccttcact
     1321 ccggtgtcct ggattccctg ctcgatgtca tggacgaaaa gtgaagcgat gtagtcggca
     1381 tcgcggttga gcaggaactg ggggagatag tcgtaggtgt agatcccggt acagagaacg
     1441 atctgga
//
LOCUS       DAOTIP010000614         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_105981_length_1445_cov_3.023022, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000614 DAOTIP010000000
VERSION     DAOTIP010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105981_length_1445_cov_3.023022"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="P5138_10440"
     CDS             complement(<1..700)
                     /locus_tag="P5138_10440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRV61039.1"
                     /translation="MEPRLTIAIPLHGGAKWIEGILDTVAKAPEWSRVVLSDATHLDD
                     AVESLAGACRGDDRVEAISRPETLGWVDHANLLLEEAQTDYFCWMPQDDLVSPEEYFE
                     LLVAALDENPDRILAFPTVLRELEVGRLRQRSIGEVPYQSPPFELGEEPPELEAVRML
                     REWNMALGCWRGVFRTRLARPIPQTDDCADVAWTFSMALEGNFIGVEQARYLKRFHGK
                     NAHHSMRWQGMPEAL"
     gene            complement(702..>1445)
                     /locus_tag="P5138_10445"
     CDS             complement(702..>1445)
                     /locus_tag="P5138_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61040.1"
                     /translation="PGMAEGHSGCWFETPERAVDPRALSKFITEAASREPLVEIRSGH
                     RVAHVERLEGGFALEAETASGNRRIESDNVINCAWDGRPALDGMILGDQSHESFRVKH
                     QVMIHGGETAGIIPATLVQGPYGDVVLWPNGDVYISWYPIARTHFGDRPEDGLEIDPV
                     VADRVHEAIVDLFPSLAGFKVLSHAPCYILAKGATDIHDPGSELHVRLGAEYVESDGW
                     FSIRSSKLTTGPLAGERCAAAITGTDPGY"
BASE COUNT          258 a          485 c          433 g          269 t
ORIGIN      
        1 ccaacgcttc cggcattccc tgccaacgca tcgagtggtg ggcgttcttt ccgtggaacc
       61 gtttcagata acgcgcctgt tcgaccccga tgaagtttcc ctccagggcc atcgagaatg
      121 tccaggcaac atcggcacaa tcgtcggttt gcgggatggg ccgcgccaga cgagtcctga
      181 atacccctct ccagcaaccc agggccatgt tccattccct cagcatccgg actgcctcaa
      241 gctcgggcgg ttcctcaccg agctcgaacg ggggagactg gtagggcact tcgccgatcg
      301 acctctggcg cagacgcccc acctcgagtt ccctgagaac cgtcgggaag gcaaggatcc
      361 ggtcggggtt ctcatcaagt gccgcgacga gcaactcgaa gtactcctcc ggcgagacca
      421 ggtcatcctg cggcatccag cagaagtagt cggtttgcgc ttcctcgagc agcaggttcg
      481 cgtgatcgac ccagcccagg gtttccggtc gcgaaatcgc ttcgacccgg tcgtcacccc
      541 ggcaagcgcc ggcaagggat tcgaccgcgt cgtccagatg ggtggcatcg ctcagcacga
      601 cccggctcca ttccggcgct ttggcaaccg tgtcaaggat tccttcgatc catttcgcgc
      661 ccccgtggag cggaatcgcg attgtcaggc gcggttccaa ctcagtaacc gggatcggtc
      721 ccggtgatcg cggcggcaca gcgctcaccg gcgagaggcc ccgtggtcag cttcgatgaa
      781 cggatcgaga accatccgtc ggactccaca tactcggcgc caagccttac gtggagctcg
      841 ctccccggat cgtgaatgtc ggtagcgccc ttggccagga tgtagcaggg cgcgtggctc
      901 agaactttga acccggccag ggacgggaac aggtccacga tcgcctcgtg gacccggtca
      961 gcgactacgg ggtcgatctc gagcccgtct tccggccggt caccgaaatg ggttcttgcg
     1021 atcgggtacc agctgatgta aacgtcgccg ttcggccaga ggaccacgtc cccgtacgga
     1081 ccctggacca gggtcgccgg aatgattccc gccgtttctc cgccgtggat catgacctga
     1141 tgcttgaccc gaaagctctc atgcgactgg tctccgagga tcattccgtc cagtgccgga
     1201 cgaccgtccc aggcgcagtt gatcacgttg tccgactcga tccttcggtt gccggaagcc
     1261 gtctccgcct caagggcgaa accgccttcg agtctttcga catgcgccac gcggtgcccg
     1321 ctccgaatct ccaccagggg ctcgcggctc gcggcttcgg tgatgaactt ggaaagggca
     1381 cgcgggtcga ccgcccgttc cggagtctcg aaccagcaac ccgaatgacc ctcggccatg
     1441 ccggg
//
LOCUS       DAOTIP010000615         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_106281_length_1442_cov_3.400144, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000615 DAOTIP010000000
VERSION     DAOTIP010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106281_length_1442_cov_3.400144"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="P5138_10450"
     CDS             <1..299
                     /locus_tag="P5138_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61041.1"
                     /translation="FSAGIAFRFRSLQAGPLMQMPVFIALFLAPVYVPQELLTGWIEP
                     VARLNPVTALLNGARSLMAGDPTGILLAYGVAFGLIALLALWALRGLRRAEVAG"
     gene            359..>1442
                     /locus_tag="P5138_10455"
     CDS             359..>1442
                     /locus_tag="P5138_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61042.1"
                     /translation="MSKVEIRRVESKKDLKKFVKVPFAVHRDHPEWVPPLIMDRMDFL
                     NQKKNPYFDHAEVELWIAEKDGEPVGRISAQIDQNWDEYRGGKDGQFGFFETTDDQEV
                     ATALLDTGSEWLAGKGREKVYGPMDFTTNDEIGIQISGFDLKPVILENCHQPYFQERV
                     EGAGFTKAMDLLMWHLEMGKLARGLEFHPAIMEAAQQSLDEHGITIRNMRRSDLANEM
                     ERFHQVYNEAWGDNWGFVPITREEVEFHAKTLKLVIDEDWAMIAETDDGETVGAALTL
                     PDINQVLAKMGGKVFPTGWFHFLTGRRKIDQVRIFALGVKKAYQHTGVAAALYVKHIQ
                     TTDPDGIMKGDAGWILETNEPMNRALE"
BASE COUNT          272 a          462 c          475 g          233 t
ORIGIN      
        1 tcttctcggc cgggatcgcc tttcgcttcc ggtcgctgca ggccggtccg ttgatgcaga
       61 tgccggtctt catcgcgctg ttcctggctc cggtgtatgt gccgcaggaa ctcctgacag
      121 ggtggatcga gccggtggcc cggctcaacc cggtgaccgc cctgctcaac ggcgcccgca
      181 gcctgatggc cggggatccg accggaatcc tgctcgccta cggggtcgcc ttcggcctga
      241 tcgcattgct ggcgctctgg gccctgcgcg gcctgcgccg ggcagaagtc gccggctgag
      301 ctcccggcct ctcgcgccgg ggagggactt ggcggccggg tccgctagtt tcccggcaat
      361 gtcgaaggtg gagattcgcc gcgtcgaaag caagaaggac ctcaagaagt tcgtcaaggt
      421 ccccttcgcg gtccatcgcg accacccgga atgggtgccg ccgctgatca tggaccggat
      481 ggatttcctc aaccagaaga agaacccgta cttcgaccac gcggaagtcg aactctggat
      541 tgccgagaag gacggtgagc cggtcggccg gatcagcgcc cagatcgacc agaactggga
      601 cgagtaccgc ggtggcaagg acggccagtt cggcttcttc gagacgaccg acgaccagga
      661 agtcgccacc gcgctgctcg acaccggcag cgaatggctg gccggcaagg ggcgcgagaa
      721 ggtctacgga ccgatggact tcaccaccaa cgacgagatc ggcatccaga tctccggctt
      781 cgacctgaag ccggtcatcc tcgagaactg ccatcagccc tacttccagg agcgggtcga
      841 gggagctggc ttcaccaagg ccatggactt gctgatgtgg catctcgaga tgggcaagct
      901 ggcccgaggc ctcgagttcc acccggcgat catggaggcg gctcagcaga gcctcgacga
      961 gcacgggatc acgatccgga acatgcgccg ctccgacctc gccaacgaga tggaacgctt
     1021 ccatcaggtc tacaacgagg cctggggcga caactggggt ttcgtgccga tcacccggga
     1081 agaggtcgag ttccacgcca agaccctgaa gctggtgatc gacgaggact gggcgatgat
     1141 cgccgagacc gacgacggcg agacggtagg tgccgctttg accctgcccg acatcaacca
     1201 ggtgcttgcg aagatgggcg gcaaggtctt cccgacgggc tggttccact tcctgaccgg
     1261 ccgccggaag atcgaccagg tgcggatttt cgccctcggc gtcaagaagg cctaccagca
     1321 caccggggtg gcggcggcgc tctatgtcaa gcacatccag accaccgacc ctgacgggat
     1381 catgaagggc gacgccggct ggatcctcga gaccaacgag ccgatgaacc gggccctgga
     1441 gg
//
LOCUS       DAOTIP010000616         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_107647_length_1430_cov_2.396364, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000616 DAOTIP010000000
VERSION     DAOTIP010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107647_length_1430_cov_2.396364"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1430)
                     /locus_tag="P5138_10460"
     CDS             complement(<1..>1430)
                     /locus_tag="P5138_10460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRV61043.1"
                     /translation="RTAGLLLLAITAVGLAIRMIAWDQPVLGDELSTLWIVRHFDLAG
                     TVREVSSDAEISPPLYFVLAWLASKLGSAPELVRLPALLAGVAAIPLSYLLGLRLRGR
                     LAGLIAAAVMALSPFMVAFSVNGRAYSLMVALLIGSTLAMLQAMRGGRPGWWVAFGLM
                     TCLAMYSHYTAIYFLAAQLIWLLVVSPGSRLPALAATAGAVVLYLPWVPSMLDDLDSP
                     TLPILEAIQGTGITEKWLGIKQLLAGQPLVDPTLLPLLLLAIGAAVVLAGFLINRKPG
                     SSPGRSPDPAAIPTPNQGLALLLALTFVTAICEALLAVIGTDTFGSRNLAATWAGLPA
                     LVGVLAASCSPAVGLTGVALLLAGFGVGTARVVDTGTATLDFKAAAEWIDENAGPDDV
                     VYDATFVSPVPLTSLDAYLPQDRDEFRPRVPSDEPPFLLAAGPDGQDQKVLDEALAAT
                     GRNRLFVVTSRDPVSAGDEAELFTTD"
BASE COUNT          276 a          459 c          518 g          177 t
ORIGIN      
        1 cgtcggtggt gaacagttcc gcctcgtctc cggcggagac cggatcacgg ctggtcacca
       61 cgaagagccg gttgcggccc gttgcggcca gggcctcatc cagcaccttc tgatcctggc
      121 cgtcggggcc ggccgccagc aggaagggcg gctcgtcgga gggaacgcgg ggccggaact
      181 catcccggtc ctgcgggagg taggcgtcga gcgaggtgag cggcacgggg ctgacgaagg
      241 tcgcgtcata gaccacgtca tcggggccgg cgttctcgtc gatccattcc gccgcggcct
      301 tgaagtccag tgtggctgta cccgtatcga ccaccctcgc ggtcccgacc ccgaagccgg
      361 ccaacagcag ggcgacaccg gtcagaccca cagccggaga acaactcgcg gccagcaccc
      421 cgaccagggc cggcagaccg gcccaggtcg cagcgagatt gcgggaaccg aaggtgtcgg
      481 tgccgatcac ggccagcagc gcctcgcaga tcgcggtcac gaaggtcaat gcgaggagca
      541 gggccagccc ctggttcgga gtggggatgg cagcaggatc cgggctgcgg cccggtgacg
      601 accccggttt ccggttaatg aggaaaccgg ccagcaccac ggcagcaccg atggccaaca
      661 gaagcagcgg cagcagggtc ggatcgacca gaggctgccc ggccagaagc tgcttgatac
      721 ccagccactt ctccgtgatt ccggtcccct ggatcgcctc gaggatcggc agggtcggcg
      781 agtcgaggtc gtccagcatc gaagggaccc acggcaggta gaggaccacc gctcccgcgg
      841 tcgcggccag ggcaggcagc cgcgatccgg gtgagacgac cagcagccaa atcagctggg
      901 cggcgaggaa gtagatcgcc gtgtagtggc tgtacatcgc gagacaggtc ataaggccga
      961 aggcaaccca ccagccgggc cggcctcctc tcatggcctg gagcatcgcg agagtggaac
     1021 cgatcagcag cgcgaccatc agcgaatagg cacggccgtt gaccgaaaag gcgaccatga
     1081 acgggctgag ggccatgacc gccgcggcga tcagtccggc cagccgtccg cggagtcgca
     1141 gcccgagcag gtagctgagg gggatcgccg cgactcctgc gagtagggcg ggcagccgga
     1201 ccagctccgg ggccgagccg agcttgctgg ccagccaggc cagaacgaag tagagcggcg
     1261 ggctgatctc ggcgtcggag gacacctcgc ggacggtccc ggcgagatcg aaatggcgga
     1321 cgatccagag ggttgaaagt tcgtcaccga ggaccggctg gtcccaggcg atcatccgga
     1381 tggcaagacc gaccgccgtg atcgcgagga gcaggagacc ggcggtgcgg
//
LOCUS       DAOTIP010000617         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_108220_length_1425_cov_2.840876, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000617 DAOTIP010000000
VERSION     DAOTIP010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108220_length_1425_cov_2.840876"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1425
                     /locus_tag="P5138_10465"
     CDS             <1..>1425
                     /locus_tag="P5138_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV61044.1"
                     /translation="SYLARAGLSVTVLESSHSPGGCIYTEEQPSGNRLELGAYEHGGI
                     RGSGVAEELELESKYGLSFHERDEFLFAPCDDGTGLPFWNSLDRTVDALADLLGREEA
                     ERYRAFSKWSAAAMGVLGQTENGPPPSIRSLASLADLTLGAEGARLMQALFAPATAVT
                     EAALGDDRLRGPLDHWAAHSQQPPQAPGTGAGALFLAASHGSPASRPAGGSVGTINAL
                     VSCLEDAGGKLVCDAPAEKVETNGGRATAVIAAGQRYEAGKAVVSAIDARRLFLGLMD
                     ESQLPAPLLAEVRRIHFSQHNVSELKVDAVIAEVPKVNGPEGMEKAFMLSANTGTDIA
                     KAFASLGLGQLPERPPVMIAFPSTLESGWAPEGQGVVWLSTFVSWTPERGQWDEALLE
                     EASELTWKTAEKALGWQMTPVEKKMTGPLEWVERHGNPGANPNHIEMSIDQLMAMRPS
                     VNLSGYSTPIGSLYLTGAGTHPGGG"
BASE COUNT          237 a          474 c          486 g          228 t
ORIGIN      
        1 tcctaccttg cccgggccgg tctctcggtg acggtgctcg agtcctccca ctctcccgga
       61 ggctgcatct ataccgagga gcagccttcc ggaaaccgcc tcgagcttgg cgcctacgag
      121 cacggcggca ttcgcgggag cggggtggcg gaggagctcg aactggagtc aaagtacggg
      181 ctgagcttcc acgaacgcga cgagtttctc ttcgccccat gtgacgacgg aaccggcctg
      241 ccattctgga attcgctcga ccgcacggtt gacgcactgg ccgacctgct cggccgcgag
      301 gaagccgagc gttaccgggc cttctcgaag tggtccgcag cggcaatggg cgtgctcggc
      361 cagaccgaga acgggccgcc gccaagcatc cgctcgctcg cgtccctggc cgacctgacc
      421 cttggcgcgg aaggggcacg gctgatgcag gccctgttcg cgccggccac tgcggtcacc
      481 gaagcggcac tcggggacga tcgcctgcgc ggcccgctcg accactgggc tgcccattcc
      541 cagcagccgc cgcaggcccc aggcaccggc gcaggggccc ttttcctcgc cgcctctcat
      601 ggttcaccgg ccagtcgtcc ggccggcggc tcggttggca cgatcaatgc cctggtcagt
      661 tgcctggagg acgccggggg aaagctggtc tgcgatgccc cggccgagaa agtcgagacg
      721 aacggcggcc gggccaccgc ggtgatcgcc gccggccaga gatacgaggc aggcaaggca
      781 gtcgtttccg ctatcgatgc ccgaaggctt ttcctgggcc tgatggacga gagccagttg
      841 ccggcgccgc ttctcgcaga ggttcgccga atccacttca gccagcacaa cgtctcggag
      901 ctcaaggtcg acgcggtgat tgcggaggtg ccgaaggtca acggtccgga gggcatggag
      961 aaggccttca tgctttctgc gaacaccggt accgacatcg cgaaggcctt cgcgagcctc
     1021 ggtctcggcc agttgcccga gcgaccgccg gtgatgatcg ccttcccctc gaccctcgag
     1081 tccggatggg ccccggaggg acagggcgtg gtctggctct cgacatttgt gtcctggacc
     1141 ccggagcggg ggcagtggga cgaggccctg ctggaagagg cgtcggagct gacctggaag
     1201 acggccgaaa aggcgctcgg ctggcagatg accccggtcg agaagaagat gaccggtccg
     1261 ctcgaatggg tggagcgcca cggcaacccc ggggccaatc cgaatcacat cgagatgagc
     1321 atcgaccagc tgatggcgat gcggccatcg gtgaaccttt ccggctattc gactccgatc
     1381 gggtctctct acctgaccgg ggcgggcacc cacccaggtg gcggt
//
LOCUS       DAOTIP010000618         1419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_108939_length_1419_cov_3.265396, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000618 DAOTIP010000000
VERSION     DAOTIP010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108939_length_1419_cov_3.265396"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="P5138_10470"
     CDS             <1..349
                     /locus_tag="P5138_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61045.1"
                     /translation="QEVIAEKQPEIAPLASRLNHLLPPRLGGPLAILDEASNGTDIVF
                     CGHVGFEGLRTVADVMAGVLVHRHIKIRFWRFKGSEIPQGDQARTEWLYDKWQMIDDW
                     VGEELAARGETDD"
     gene            complement(346..>1419)
                     /gene="aroA"
                     /locus_tag="P5138_10475"
     CDS             complement(346..>1419)
                     /gene="aroA"
                     /locus_tag="P5138_10475"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRV61046.1"
                     /translation="FTIEGVGPSGPRSLGRIDVGNAGTLMRLLPGWLAGCDGGLWRMN
                     GDASIRKRPVDRIAIPLREMGAEIEVTDGRFPPIRIDGRQLTGITYELPVASAQVKSC
                     ILLAGLRAEGPTTVVEPVATRDHTEVMLAAAGADIAREPGRVTINPGAGLRLDEVEVP
                     ADISSAAFFLVAASIVPGSEVVIERCGVNPTRTGIIDILRRMGADIELEPQAGAEGEP
                     IARITVRWAPLKGTRIEGAEIPLAIDELPVIALAACFAEGETVIADAEELKAKESDRI
                     AVVVGALAGFGANIEARDDGMRIVGTGGLKGGQVDSHGDHRIAMLGAVAGIAGEEGVT
                     VDGMDAAAVSYPTFQQDLARLIG"
BASE COUNT          228 a          508 c          454 g          229 t
ORIGIN      
        1 ccaggaagtg atcgccgaga agcaacctga aatcgccccc cttgcttccc gcctgaacca
       61 cctgctgccg ccccgcctcg gcggcccgtt ggcgatcctg gacgaggcaa gcaacgggac
      121 cgacatcgtc ttctgcggcc acgtcggctt cgagggcctg cgcaccgtcg ccgacgtcat
      181 ggccggggtg ctggtccacc gccacatcaa gatccgcttc tggcgtttca agggaagcga
      241 gatccctcag ggcgatcagg cccgtaccga gtggctctac gacaagtggc agatgatcga
      301 cgactgggtc ggcgaggaac tcgccgcccg cggcgaaacc gacgactagc cgatcaacct
      361 ggcgaggtcc tgctgaaagg tcgggtagct gactgccgca gcgtccatcc cgtcgaccgt
      421 gaccccttct tcgccggcga tgcctgccac cgcgccgagc atggcgatcc ggtggtctcc
      481 gtgagaatcg acctgcccgc ccttgaggcc accggtaccg acgatccgca tgccgtcgtc
      541 gcgggcttcg atattggctc cgaatccggc gagagctcca accaccactg cgatccggtc
      601 ggattccttg gccttcaact cctcggcatc ggcgatcacc gtctcgccct cggcgaagca
      661 ggcggccagc gcaatgaccg gcaactcgtc gatcgccagg ggaatttccg ctccctcgat
      721 tctggttcct ttgaggggcg cccaacgaac ggtgatccgg gcgatcggct ccccctccgc
      781 cccggcctgc ggctcgagtt cgatgtccgc gcccatccgg cggaggatgt cgatgatccc
      841 ggtcctggtc gggttcacgc cgcagcgctc gatcacgacc tcggaaccgg gaacgatcga
      901 ggcggcgacc aggaagaagg cggcagaaga gatgtcggcc gggacctcga cctcgtcgag
      961 ccggaggccg gcaccgggat tgatggtgac ccggccgggc tcgcgggcga tgtccgcccc
     1021 ggccgctgcc agcatcactt cggtgtggtc ccgcgtcgcg accggctcga ccacggtggt
     1081 cggcccttcc gccctcagcc cggccagcaa gatgcatgac ttgacctggg ccgaagcgac
     1141 gggaagttcg taggtgatgc ccgtaagttg acgcccgtcg atcctgatcg gggggaaccg
     1201 cccgtcggtg acctcgatct ccgctcccat ttcgcgcagc ggaatcgcga tccggtccac
     1261 cgggcgcttg cggatcgagg catcgccatt catccgccag agaccgccgt cgcagccggc
     1321 cagccagccc ggcagcagcc gcatcagggt tcccgcgttg ccgacgtcga tccggccgag
     1381 gctgcgcggt cctgacgggc cgaccccctc gatcgtgaa
//
LOCUS       DAOTIP010000619         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_109210_length_1417_cov_2.198972, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000619 DAOTIP010000000
VERSION     DAOTIP010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109210_length_1417_cov_2.198972"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="P5138_10480"
     CDS             complement(<1..433)
                     /locus_tag="P5138_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRV61047.1"
                     /translation="MSSRAFSRRGAVHARKGPSARGQAPRGASPVGRTSELARKRERR
                     SFRFRLVAFFGLILVLLLAVGYQFWLRDSSLVGIKNLEVVGVSSRTEEGRQIDQAVRT
                     AMGEMTTLHVQQAILDQELERFPRVSSAIIETSFPDSATVTV"
     gene            complement(430..1194)
                     /locus_tag="P5138_10485"
     CDS             complement(430..1194)
                     /locus_tag="P5138_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRV61048.1"
                     /translation="MITNIEMDHHARWENLAELRRAFHDFAAKARAVTLPAGDPDLAA
                     GLASEERQITPFDLGRPGPPELGLSVPGEHNRLNARAAIGALTAAGFDAADAAEALRS
                     FTGVKRRQEFKGEFDGTRVYDDYAHHPTEVRAALEAIREFDPDRLIAVFQPHLYSRTK
                     IFSQQFGAALARADEIVVLDVYPAREEPVGPLAGVSGLDVLRAAADRAGGRPAYWAPD
                     LDSAERIVRGRAADGAVVVTLGAGDVTRLSDRLVAK"
     gene            1165..>1417
                     /locus_tag="P5138_10490"
     CDS             1165..>1417
                     /locus_tag="P5138_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HRV61049.1"
                     /translation="MMIHLDVCDHGDLGVEGQVTIELADVPAPEALDRVLAELPSEYV
                     YKMRGSTLIVAAPEKLVEILPACLIEDELVSRPEPPPESI"
BASE COUNT          231 a          477 c          477 g          232 t
ORIGIN      
        1 ggacggttac cgtggccgag tccgggaagg aggtctcgat gatcgcgctc gagaccctgg
       61 ggaagcgctc caactcctgg tcgagaatcg cctgctggac atgcagggtc gtcatctccc
      121 ccatcgcggt ccgcaccgcc tggtcgatct ggcgaccctc ttcggtacgg gagctcactc
      181 cgaccacctc gaggttcttg atccctacga gagacgaatc acgcagccag aactggtagc
      241 cgacggcgag caggagcacc aggatcaggc cgaagaaggc gacaagtctg aagcggaagc
      301 tgcgtcgttc acgctttcgc gccagttcgc tggttcgacc caccgggctg gccccccgcg
      361 gggcctggcc ccgggccgaa gggcccttcc gcgcgtgaac ggctccccgg cggctgaatg
      421 cccggctgct catttcgcga ccagcctgtc cgagagtcgg gtcacgtccc ctgcaccgag
      481 cgtgaccaca accgctccgt cggcggcccg gccacggacg atccgctcgg ctgaatcgag
      541 gtcgggggcc cagtaggccg gccgtccccc ggcccggtcg gcggctgccc gaagcacgtc
      601 aagcccgctg actccggcca gcggcccgac cggttcctcc cgggcgggat agacgtcaag
      661 cacgacgatc tcgtcggccc gggcgagggc ggcaccgaac tgctggctga agatcttggt
      721 ccgggaatag aggtggggct ggaacacggc gatcagccgg tccgggtcga actcgcggat
      781 cgcctcaagt gcggcgcgca cttcggtcgg atggtgggcg tagtcgtcat agaccctggt
      841 gccgtcgaat tcgcccttga attcctgccg gcgcttgacc ccggtaaagc tccgcagggc
      901 ttccgccgcg tcggccgcat cgaagcctgc tgccgtcagc gccccgatcg ccgcccgcgc
      961 gttgagccgg ttgtgctcac caggaaccga aagacccagc tcgggcggcc ccggtcgacc
     1021 gaggtcgaag ggcgtgatct gcctttcttc ggaggccagt ccggcggcca ggtcggggtc
     1081 accggccggc agggtcaccg ccctggcctt ggccgcgaag tcatgaaacg cccggcgcag
     1141 ttcggccagg ttttcccagc gggcgtgatg atccatctcg atgtttgtga tcacggcgat
     1201 ctcggggtcg agggtcaggt caccatcgag ctagcagatg tgccggcgcc cgaagccctc
     1261 gaccgggtat tggccgagct gccgagtgag tatgtttaca agatgcgggg aagcacgctg
     1321 atcgtggccg cccccgagaa gctcgtcgag attctaccgg cttgcttgat cgaggacgag
     1381 ttagtgagcc gcccagaacc gccacccgaa agtattc
//
LOCUS       DAOTIP010000620         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_109264_length_1416_cov_3.924320, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000620 DAOTIP010000000
VERSION     DAOTIP010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109264_length_1416_cov_3.924320"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="P5138_10495"
     CDS             complement(<1..556)
                     /locus_tag="P5138_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61050.1"
                     /translation="MRAAQVTAYGDAPQLIEIDPPGDPGPCGESVAGPAEVVEVLVAG
                     INPVDIAIGSGKFDAGAPPLPYVPGMEGIGRMADGEVVWFEGAHFPWGSMAERAVITE
                     GSGITLPSGIEPERAIGFGVAGMAAWLSVDWRGQLKDGETVLILGASGSVGQVAVQAA
                     RMMGAGRVVGMARSESGREKVLALG"
     gene            complement(553..>1416)
                     /locus_tag="P5138_10500"
     CDS             complement(553..>1416)
                     /locus_tag="P5138_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV61051.1"
                     /translation="NRLHKAKLRPGPHDGIDGMEKIDKIINIDQSPIGRTPRSNPATY
                     TGVFDQIRQLFAATQESRARGYKPGRFSFNVKGGRCEVCKGDGQIKIEMHFLPDVYVP
                     CEQCHGHRYNRETLEVRFKGKSIADVLEMSIEEAYPFFESIPKVARRLKTLNDVGLGY
                     VRLGQPATTLSGGEAQRIKLAAELSKVATGNTLYILDEPTTGLHFGDVRRLLEVLGRL
                     VDAGNTVVVIEHDLDVIKSADWIVDLGPEGGDGGGHIVASGTPEEVAGVQGSYTGDFL
                     RGVLEPVALAA"
BASE COUNT          224 a          493 c          446 g          253 t
ORIGIN      
        1 caccgagggc gagcaccttc tcccgcccgg actcgctgcg cgccattccg accacccggc
       61 ccgcccccat catccgggcc gcctggactg cgacctgacc gaccgagccg ctggccccga
      121 ggatcaggac cgtttcacca tccttgagct ggccccgcca gtccaccgag agccaggcgg
      181 ccatcccggc cacgccgaat ccgatcgctc gctcgggttc gattccggat ggaagcgtga
      241 tcccgctgcc ttcggtgatc actgcccgct cggccatcga accccaggga aagtgggcgc
      301 cctcgaacca gacgacctcg ccgtccgcca tccggccgat cccctccatg ccgggtacat
      361 acggaagcgg tggtgctccc gcatcgaact tgcccgagcc aatcgcgatg tcaaccgggt
      421 tgatccccgc gaccaggacc tcgacgacct cggcggggcc ggcgactgac tcgccgcatg
      481 gtccggggtc tcccggcggg tcgatttcga tcaactgcgg ggcatcaccg taggcggtga
      541 cctgggcggc tctcatgcgg ccaaagctac cggctcgagc accccgcgga ggaaatctcc
      601 ggtgtaggaa ccctgcactc cggccacctc ttccggggtg ccgctggcga cgatgtggcc
      661 gcctccgtcg ccgccttcgg ggccgaggtc gacgatccag tcggcgctct tgatcacgtc
      721 caggtcatgc tcgatcacga cgaccgtgtt tccggcgtcg accaggcgtc ccagcacctc
      781 gagcagacgg cggacgtccc cgaaatggag gcccgtggtc ggctcgtcaa ggatgtagag
      841 ggtgttgccg gtcgccacct tcgaaagctc ggcagccagc ttgatgcgct gggcctcgcc
      901 accggaaagg gtggtcgcgg gctggcccag gcggacgtat ccgagcccga cgtcgttgag
      961 cgtcttcagc ctgcgggcga ccttgggaat cgactcgaaa aacggataag cctcttcgat
     1021 cgacatctcc agcacgtcgg cgatcgactt gcccttgaac cggacctcga gggtctcgcg
     1081 gttgtaacga tgcccgtggc attgctcgca ggggacatac acgtccggga ggaaatgcat
     1141 ttcgatcttg atctggccgt cgcccttgca gacctcgcaa cggccgccct tcacgttgaa
     1201 gctgaagcgg cccggcttgt atccgcgggc ccgcgattcc tgggtcgccg cgaacaactg
     1261 gcggatctgg tcgaagaccc cggtgtaggt ggccgggttc gagcgcgggg tccgaccaat
     1321 cggtgactgg tcgatgttga tgatcttgtc gatcttctcc atgccgtcga ttccgtcatg
     1381 cggacctggc cttagcttcg ccttgtggag ccggtt
//
LOCUS       DAOTIP010000621         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_109279_length_1416_cov_3.122704, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000621 DAOTIP010000000
VERSION     DAOTIP010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109279_length_1416_cov_3.122704"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="P5138_10505"
     CDS             <1..497
                     /locus_tag="P5138_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRV61052.1"
                     /translation="VADVIQIGARNMQNYALLSEAGKSGKAVLLKRGLSATIEELLMA
                     SEYILKEGNENVMLCERGIRTYETAYRFTLDLLAVPALKEQTHLPVIVDPSHAPGRRD
                     WVESMSLAAAASGADGIIIEVHNEPEKAICDAAQQVPTSEFQQLTNKLETITSVVGKT
                     ISRA"
     gene            517..>1416
                     /locus_tag="P5138_10510"
     CDS             517..>1416
                     /locus_tag="P5138_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRV61053.1"
                     /translation="MKLAVLGVGLIGGSIGLAARDRGLAEVTGWVRNPDRAEECLKVG
                     AVDHMAATVEEACADADVVVCCASVGHLIEMGQKALEAAPEDCVVTDVGSTKQELVEA
                     LGGDRRFIGGHPLAGAETAGVANARADLYEGARWYLTPTSESEGMLLDRLQKLVKGLG
                     ATPVAIDAAEHDRAMAVVSHLPHVFANQLAAQGRPGTERLGALAPSLRDGTRVAGSNP
                     AIWGEIFATNHEAVVAAIEESIAGLETAREVIGSADPERIEKWQAAVGEDRAALSATE
                     SDLGATHELRVLVPNQPGVLAELA"
BASE COUNT          249 a          450 c          505 g          212 t
ORIGIN      
        1 aggtcgctga tgtgatccag atcggggccc gcaacatgca gaattacgcc ctgctctccg
       61 aagcgggcaa gtccggcaag gcggtgctgc tcaagcgcgg tctctcggcc acgatcgagg
      121 agctgctgat ggcttccgag tacatcctca aggaaggcaa cgagaacgtg atgctctgtg
      181 agcgcgggat ccgcacctac gaaacggcct accgcttcac gctcgacctg ctggccgttc
      241 cggccctcaa ggagcagacg cacctgccgg tgatcgtcga tccgagccat gcgccgggac
      301 gacgcgactg ggtcgagtcg atgtccctgg ccgcggcggc atcgggcgcg gacgggatca
      361 tcatcgaggt tcacaacgag cccgagaagg cgatctgcga cgcagcccag caggttccga
      421 cctccgagtt ccagcagctg acgaacaagc tggagacgat cacctcggtt gtcggcaaga
      481 cgatctcgcg ggcctagagg cgccggggca tctgaaatga aactggccgt cctcggagtc
      541 gggctgatcg gaggctctat cggcctcgca gcccgggacc gtggcctggc cgaagtcacc
      601 ggctgggtcc ggaacccgga ccgggccgag gaatgcctca aggtcggggc ggtcgatcac
      661 atggccgcca ccgtagaaga ggcctgcgcc gatgccgacg tggtggtctg ctgcgcctcg
      721 gtcggtcacc tgatcgagat gggtcagaag gccctcgaag ccgccccgga ggactgcgtg
      781 gtgaccgacg tcggatcgac caagcaggag ctggtcgagg cgctcggcgg agaccgccgg
      841 ttcatcggtg gccacccgct ggccggagcg gagaccgccg gggtggccaa tgccagggcc
      901 gacctctacg aaggggcgcg ctggtacctg accccgacct ccgagtcgga agggatgctg
      961 ctggaccgac tgcagaagct ggtcaagggc ctgggcgcga cgccggtcgc gatcgatgcc
     1021 gcagaacacg atcgcgcgat ggccgtggtg agtcatctgc cgcatgtatt tgccaatcag
     1081 ctcgccgccc agggccggcc gggcaccgaa aggctggggg cgctcgctcc gagcctgcgt
     1141 gatggcacca gggtcgccgg ctcgaacccg gcgatctggg gcgagatctt cgccaccaat
     1201 cacgaggcgg ttgtggccgc catcgaggag tcaatcgccg gccttgagac tgcccgggaa
     1261 gtgatcggct cggccgatcc ggagcggatt gagaaatggc aggcggcggt tggtgaagac
     1321 cgggcggctc tctcggcgac cgagtccgat ctcggcgcca cccatgagct gcgggtgctg
     1381 gtcccgaacc agcccggggt gctggccgaa ctggcc
//
LOCUS       DAOTIP010000622         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_109281_length_1416_cov_3.101396, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000622 DAOTIP010000000
VERSION     DAOTIP010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109281_length_1416_cov_3.101396"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1087)
                     /locus_tag="P5138_10515"
     CDS             complement(236..1087)
                     /locus_tag="P5138_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV61054.1"
                     /translation="MGENVRSEIFRDGLLDGKVVVLSGAGTGLGREATLEMVRLGATV
                     VGCGRRPEPLEETADLAAGLPGKFEHGTLDIRDEEAVDGFFDGVVERHGRIDVLVNNA
                     GGQFLSPAEMISPKGFRTVIELNVTGTWLMTHAAATKAFIPQESGAILNVTLSPHNGM
                     PGMVHSGAARAAVENMTKTLSIEWSRFNIRTCAIAAGQFATDTFVNKYPKEIVESVHN
                     TIPTGRVGESEEMGWLIAFLASDAGAFHSGSVITLDGARDNWFGPWPPAAIASEGGSV
                     PAEERRE"
     gene            1138..>1416
                     /locus_tag="P5138_10520"
     CDS             1138..>1416
                     /locus_tag="P5138_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61055.1"
                     /translation="MSIHAESRPLDTPLPGGKDGATVTVEPMVTGTFSSPPILHDSPK
                     GKAGTFAILRSTRKEREPTPIPCFLIRHPSAGLILVDTGLHPSIASDPA"
BASE COUNT          240 a          515 c          419 g          242 t
ORIGIN      
        1 gatgtaggca cttccctgca ccatcttcga cggagcccga accgctttca tgtgagggct
       61 tggtgcagcc tgggcggggg cgaccgaccc gagcaggagg aggcatgaga ccagcatggc
      121 ggtcccgccg aaccggaaaa gcactttctt ccttccctga accctgctca cgcgcctgca
      181 cctcccgaag aacgacttct gaccgagcat agcgccaccg cctgcccgac tcccactatt
      241 cgcggcgttc ttccgccggg acggagccgc cctcgctggc gatggcggcc gggggccagg
      301 gcccgaacca attgtcacgt gccccgtcca gggtgatcac cgaacccgag tggaaggctc
      361 ccgcgtcgga agcgaggaag gcgatcagcc agcccatctc ctcggactcc cccacccttc
      421 ccgtcgggat cgtgttgtga accgactcga cgatctcctt cggatacttg ttgacgaagg
      481 tgtcagtggc gaactggccg gccgcgatcg cgcaggtccg gatgttgaac ctcgaccact
      541 cgatcgaaag cgtcttcgtc atgttctcga ccgcagcccg ggccgccccg gaatggacca
      601 tcccgggcat cccgttgtga ggtgaaaggg tcacgttcag gattgcccca ctctcctgcg
      661 ggatgaaggc cttggtggct gcggcatggg tcatcagcca ggtgccggtc acgttcagtt
      721 cgatcaccgt gcgaaaaccc ttggggctga tcatctcggc cgggctgagg aactggcctc
      781 ccgcgttgtt gaccaggaca tcgatccgcc cgtggcgttc gaccaccccg tcgaagaacc
      841 cgtcgaccgc ttcttcgtcg cggatatcga gggtcccgtg ttcgaacttg cccggaagcc
      901 cggccgcaag gtcagcggtc tcctcgagcg gttccgggcg acggccgcat ccgaccacgg
      961 tggcacccag ccggaccatc tccagggtcg cttccctccc cagaccggtg ccggccccgg
     1021 acaggaccac taccttgccg tcaagcagcc cgtcgcggaa aatctcggat cgtacgtttt
     1081 ctcccatcgg ctgccgcgaa tcctacccct gaccggtccg gcggtaaggt cacgcgcgtg
     1141 agcatccacg ctgaatccag accgctcgac acgcccctcc cggggggcaa agacggcgcg
     1201 accgtgaccg tcgagccgat ggtcaccgga accttcagca gcccgccgat cctgcacgac
     1261 agccccaagg gcaaggccgg caccttcgcg atcctccgct cgacccggaa ggagcgggaa
     1321 ccgaccccga ttccctgctt tctgattcgc cacccctccg ccggcctgat cctggtcgac
     1381 accggccttc acccttcgat cgcctccgat ccggcg
//
LOCUS       DAOTIP010000623         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_109518_length_1414_cov_2.885210, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000623 DAOTIP010000000
VERSION     DAOTIP010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109518_length_1414_cov_2.885210"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5138_10525"
     CDS             complement(<1..462)
                     /locus_tag="P5138_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61056.1"
                     /translation="MNLHRPGLRGKLALAIALVLLTALGITYVAVYRGTGSELSSRTD
                     NELAQEVDRISTRLESAAGEDPDAYTRRARTITSAENFGPNARVVSISIADGGTATNQ
                     PELVEPGIDRPDGGGDGSGTSGDGEHGDDDGSHEPDEKRQDARRILTASPGY"
     gene            complement(459..1133)
                     /locus_tag="P5138_10530"
     CDS             complement(459..1133)
                     /locus_tag="P5138_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV61057.1"
                     /translation="MRILVVEDERAIADFISRGLEAEGYAVTCVEDGIDGETLARTGR
                     FDLILLDIMLPGRDGLEVLSNLRREDGDTPVILLTAKGEVEDRVAGLDTGANDYIPKP
                     FAFEELVARVRAHLRRPGQGTATELEVGDLKMDLLRRQVSRAGKEILLSAREFDLLAY
                     LMRHPGQVLSREQLLNGVWGYDHDPGTNIVGVYIAYLRRKLAIDGEIDPIETLRSVGY
                     RLRDDR"
BASE COUNT          232 a          488 c          446 g          248 t
ORIGIN      
        1 gtacccgggt gacgcggtca ggatgcgccg ggcgtcctgc ctcttctcgt ccggttcgtg
       61 actcccgtcg tcgtcaccat gctcaccgtc accgctcgtg ccgctcccgt cgcctccccc
      121 gtccggccgg tcgattcccg gctcgaccag ttccggctgg ttcgtggccg tgccaccatc
      181 cgcgatcgaa atcgaaacta cccgggcatt cgggccgaag ttctcggccg aggtgatcgt
      241 cctggcccgc cgcgtgtagg cgtccgggtc ttcgcccgcg gctgactcaa ggcgggtaga
      301 aatccggtcg acttcctggg ccagttcgtt gtcggtccgg ctgctcagct cggatcccgt
      361 cccccgatag acggctacat aagtgatccc gagcgcagtc aggagcacca gcgcgatggc
      421 cagggcgagc ttgccccgga gaccgggccg atgcaggttc accggtcgtc ccgcagccgg
      481 tagccgaccg acctcagggt ttcgatcggg tcgatctcgc cgtcgatcgc cagcttgcgc
      541 cgcaggtagg cgatgtacac accgacgatg ttggtgccgg ggtcatggtc gtatccccag
      601 acaccgttga ggagctgctc gcgggagagg acctggccgg gatgcctcat caggtaggcc
      661 agcaggtcga attcgcgggc cgagagcagg atctccttgc cggcccggct tacctggcgg
      721 cgaagcaggt ccatcttcag gtcacccacc tcgagttcgg ttgcggtccc ctggccgggc
      781 cgcctgagat gtgcccggac ccgggcaacc agttcctcga aggcaaaggg tttcgggatg
      841 tagtcgttcg ctcccgtgtc cagccccgcc acccggtctt ccacttcgcc cttcgcggtg
      901 aggagtatca ccggcgtgtc gccgtcttcg cggcgcaggt tcgaaagcac ctcgaggccg
      961 tcccggccgg gcagcatgat gtccagcagg atcaggtcga accggccggt gcgggcaagg
     1021 gtctcgccgt cgatcccgtc ctcgacacag gtcaccgcgt atccctcggc ctccagcccg
     1081 cgggagatga agtcagcgat agcccgctcg tcttcgacca caagaattct catcgccgcc
     1141 gatcgtagct tcgattgcgg ggtttcccgg cattctaaga aaactctcat ctttccttac
     1201 ggaaactccc agccgcctcc ctcagtcttg tccacatgag cgaaactcca gacaacgaga
     1261 agcaggatgc aaacgaagca ggggaagaac cgacgcgcga ggcgcccacc gaggtcgccg
     1321 agacgcgcaa gcttcccacc ccggaagacg aggtgacgga gcaggcggaa gctgccggcc
     1381 cggcagttga ggacggcacg tccgcgaccg cccg
//
LOCUS       DAOTIP010000624         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_109817_length_1411_cov_4.022124, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000624 DAOTIP010000000
VERSION     DAOTIP010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109817_length_1411_cov_4.022124"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..510
                     /gene="csrA"
                     /locus_tag="P5138_10535"
     CDS             298..510
                     /gene="csrA"
                     /locus_tag="P5138_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRV61058.1"
                     /translation="MLVLTRKKEQSIIIGENVEIMVLGVSGDKVRLGITAPREVDVFR
                     KEVIDERGSHVDGSHVDGSHVEAEQA"
     gene            complement(569..802)
                     /locus_tag="P5138_10540"
     CDS             complement(569..802)
                     /locus_tag="P5138_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61059.1"
                     /translation="MRQSINQFETAFEQQKVLEQRRREQLRTRAVNRSRARRVTRSQQ
                     QGKVRFSVLAVCLIVTVVLVTIAMFEALLFFMS"
     gene            complement(946..>1411)
                     /locus_tag="P5138_10545"
     CDS             complement(946..>1411)
                     /locus_tag="P5138_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRV61060.1"
                     /translation="IGWYIQRDDETEKGPASAGSSSVVAKVDRVVDGDTAKVFVNGQD
                     EYVRYIGIDTPESVKEGAPVECYGKEASRYNEQLLARNPQVRLVFDREKRDQYGRLLA
                     YVYSGPILLQSELLRGGYATTLEIPPNTSKADQFAELESEAREAGRGLWSAC"
BASE COUNT          273 a          427 c          427 g          284 t
ORIGIN      
        1 gccagacgtt tcatctgcac ggagcgaagt ttctgctggc gccggaactc gcggagttcc
       61 gaagcgctga cttcgagctg atgggcgatc caggcatcgg tgcgtccctg gcggacccag
      121 gcccgaatct ggttgagttg ggggcgtagt gatcggcgag ccatagaggt ttgcgagtct
      181 attcaaaccg cttggatcgt cgctttgcgg ggtagtgatc agaaaggttc acaccccgtc
      241 ggccgcaata cctattatta ggtatcgaac cgaccactcg gagtcgaatg gagtcacatg
      301 ctggtactca ccagaaaaaa ggaacagagc atcatcatcg gagagaacgt ggagatcatg
      361 gttctcggcg tttccggtga caaggtgcgt ctgggcatca ccgcgccgcg ggaagtcgat
      421 gttttccgca aggaagtgat cgacgagcgc gggtctcatg tcgacggatc tcacgtcgac
      481 ggatcgcatg tagaggccga gcaggcctga agcgcaggga cccgggggct gtggggagcc
      541 tccggtttgt ccagggggcc ggtccggcct aggacatgaa gaagagcagc gcctcgaaca
      601 tggcgatggt caccaggacc acggtcacga tgaggcagac ggccagcacg ctgaagcgca
      661 ccttgccctg ctgctgggag cgcgtcaccc ttcgggcgcg cgaacggttc acagcccggg
      721 tgcgcagttg ctcacgccga cgctgctcta gaaccttctg ctgttcgaaa gcggtttcga
      781 actggttgat gctctgacgc attctcataa tggttgcgac ccggctgaaa gcgaactttc
      841 agaaacaggc cgtccgtcca ccgtagcaaa tgattggcca atctcaagcc ttcactcaca
      901 cttctgtgag gaagtcacag tattttcaca agccgccttc ggacctcagc acgccgacca
      961 gaggccgcgt ccggcttccc gcgcctcact ctccagttcg gcgaactgat cagccttcga
     1021 agtgttcggg gggatttcca gcgtggtcgc atacccgccc ctgaggagct ctgactgaag
     1081 caggatcggg cccgagtaca cataggccag cagtcgtccg tactgatccc gcttttcccg
     1141 gtcgaagacc agcctcacct gcgggttgcg ggccagtagc tgctcgttgt accggctggc
     1201 ttccttgcca tagcactcga ccggggcgcc ctccttgacc gattccggcg tgtcgatccc
     1261 gatgtagcgg acgtactcgt cctggccgtt cacgaacacc ttcgcggtgt cgccatcgac
     1321 cacccggtca accttggcca cgacagaaga agaccccgcg ctcgcggggc ctttttcagt
     1381 ttcgtcgtcc ctctggatgt accacccgat c
//
LOCUS       DAOTIP010000625         1403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_110719_length_1403_cov_2.887240, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000625 DAOTIP010000000
VERSION     DAOTIP010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110719_length_1403_cov_2.887240"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="uppP"
                     /locus_tag="P5138_10550"
     CDS             complement(<1..690)
                     /gene="uppP"
                     /locus_tag="P5138_10550"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HRV61061.1"
                     /translation="MNLIQSIFLGILQGLTEFLPISSSGHLLFVPALLGWDDPGAAFT
                     AVIQLGTEAAVLIYFRKDLWNIARDWTESIFKPEMRGELNPRMGWYLIIATIPISILG
                     LTFQNQIETAARNLWLVGSMLIIFGLVLGRADRVGSQEREVGELSFKDAVIIGLAQSL
                     ALIPGVSRSGATISAGLFLNLNREAAARFAFLLAIPAVVLSGFYELYKLLSGKEHFGE
                     PMINIAIATAFA"
     gene            complement(778..>1403)
                     /locus_tag="P5138_10555"
     CDS             complement(778..>1403)
                     /locus_tag="P5138_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV61062.1"
                     /translation="ALALAAGEGLGSVHEAIAAAAGPGVGGASGGLRICVNCAGVGAA
                     GRIVQKGEPASLDGYKWVIEINLIGTINVLSQAAAAMVGNDPDSDGQRGVCINTASIA
                     AYDGQIGQTAYAASKGGVVGLTLPAARDLSRDGVRVMTIAPGLFDTPLLALLPEPARQ
                     ALGETIPFPSRLGFPEEFASLALQIVDNVMLNGEVIRLDGALRMAPK"
BASE COUNT          245 a          447 c          499 g          212 t
ORIGIN      
        1 ggcgaaggcg gtggcgatcg cgatgttgat catcggctcg ccgaaatgct ccttgccgga
       61 gagcagcttg tagagctcgt agaagccgga gaggaccacc gccggaatgg caaggaggaa
      121 ggcgaagcgg gcggcggcct cgcggttcag gttgaggaag agtccggcgc tgatcgtcgc
      181 tcccgaacgg gacaccccgg ggatcagggc cagggactgg gccaggccga tgatcacggc
      241 gtccttgaac gagagctcac ccacctccct ttcctgggag ccgacccggt cggcccggcc
      301 gaggaccagg ccgaagatga tcagcatcga gccgaccagc cagaggttgc gggccgcggt
      361 ttcgatctgg ttctggaagg tgagaccgag gatcgagatc gggatcgtcg cgatgatcag
      421 gtaccagccc atgcgggggt tgagctcgcc ccgcatctcc ggcttgaaga tcgactcggt
      481 ccagtcccgg gcgatgttcc agaggtcctt gcggaagtag ataagcacgg ccgcctcggt
      541 cccgagctgg atcaccgcgg tgaaggcggc tccggggtca tcccagccga ggagcgccgg
      601 cacgaacagg agatgtccgc tggaggaaat cggcaggaac tcggtcagac cctgaaggat
      661 cccgaggaag atgctctgaa tgaggttcac ggggggttca ggaaatgcta ctcccgtggc
      721 cggtgggccg gatcggctgc agatgcctgc gcaatccgga ccaccgccag ggggcgatta
      781 cttcggggcc atgcgcaagg cgccgtccag gcggatcact tccccgttca gcatcacgtt
      841 gtccacgatc tggagggcga gggaggcgaa ctcctccggg aagccgaggc gcgacgggaa
      901 cgggatcgtt tcgccgagtg cctggcgggc cggttcgggc agcagggcca gcagcggagt
      961 gtcgaagagt ccgggggcga tcgtcatcac ccgcaccccg tcccgggaaa ggtcacgggc
     1021 cgccggcagg gtcagcccga ccacgccgcc cttcgaggcc gcgtaggccg tctggccgat
     1081 ctggccgtca taggcggcga tcgaggcggt gttgatgcag acgccgcgct ggccgtcgct
     1141 gtcgggatcg ttgccaacca tcgccgcggc agcctggctc aggacgttga tcgtgccgat
     1201 caggttgatc tcgattaccc atttgtagcc gtcgagtgat gcgggctcgc ccttctggac
     1261 gatcctcccg gccgccccga cgccggcgca gttcacgcag atccgcaagc cgccgctggc
     1321 accgccaaca cccgggcctg ccgccgcggc gatcgcctca tggaccgacc caaggccctc
     1381 tccggcggcc agcgccagcg cgt
//
LOCUS       DAOTIP010000626         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_110805_length_1402_cov_3.481069, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000626 DAOTIP010000000
VERSION     DAOTIP010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110805_length_1402_cov_3.481069"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1018
                     /locus_tag="P5138_10560"
     CDS             170..1018
                     /locus_tag="P5138_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRV61063.1"
                     /translation="MAERRGMGRGLGAILPESATGPDMRELPVKSIDRNPGQPRTNFS
                     EEALESLAESIRASGVVQPLIVRPAGRGKYEIIAGERRWRAAQMAGVEKVPVVIRDSD
                     EADRLEVALIENMVRENLNPVEEAKACAALVEDLGLSKEELGKRVGRSRPQISNLIRL
                     LDLPDDVLDMLEQGELTEGHGRAILQVPDHADRRKLAKQAKKEGWSVRQTESGARALA
                     TAPKKGGRKRSLSAEEEEALAGATERLETGLDHPVKIRPKGKGLVVEIELDDLAQAIE
                     IADRLG"
     gene            1107..1180
                     /locus_tag="P5138_10565"
     tRNA            1107..1180
                     /locus_tag="P5138_10565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1141..1143,aa:Ile,seq:gat)
BASE COUNT          282 a          448 c          457 g          215 t
ORIGIN      
        1 tgatgaaggg gaatagtcga tcacttctcc gagacggtgt tccggacggt gataccgagg
       61 accgtgcgcg ttgcggaggc ccccagctat ggcataccgg tgaccgacta ttccccttca
      121 tcaaggggtt ccgaggccta tctcgccctg gccgaagagg tggtggcacg tggctgagcg
      181 ccgtggaatg ggccgcggcc tgggagcgat tctgcccgag agtgccaccg gtcccgacat
      241 gcgggaactg ccggtgaaat cgatcgaccg gaaccccgga cagcctcgca ccaatttctc
      301 cgaggaagca ctcgagtccc tcgccgaatc gatccgggca agcggagtgg tccaaccact
      361 tatcgtcagg ccggccggcc gcggcaagta cgagatcatc gccggcgagc gtcgctggcg
      421 agccgcacag atggcagggg tcgaaaaggt tcccgtcgtc atccgcgact ccgacgaagc
      481 cgaccggctc gaggtcgcgc ttatcgagaa catggtccgg gagaacctga acccggtgga
      541 agaagcgaag gcctgcgccg ccctggtcga ggacctcggc ctcagcaagg aagaactggg
      601 caagcgggtc ggccgcagcc ggccgcagat ctccaatctg atccgccttc tcgacctgcc
      661 cgacgacgtc ctcgacatgc tcgagcaggg agaactcacc gaaggccacg gccgggcaat
      721 cctccaggtg ccggaccatg ccgaccgccg caaactcgcc aaacaggcga agaaggaagg
      781 ctggtcggta aggcagaccg agtcgggagc gcgggccctg gcgacggcgc cgaagaaggg
      841 gggacgcaag cgatccctct ccgccgagga agaggaagcg ctcgccggcg ccaccgagcg
      901 gctcgaaacc ggtctcgatc acccggtgaa gatcaggccg aagggcaagg gtctggtcgt
      961 ggagatcgaa ctcgacgacc tggcccaggc gatcgagatc gccgatcgcc tcggctgacc
     1021 gggggacgga caggccctgc ctgaccggcc gcgtttcacc tcacgacgcg gggacccggc
     1081 cacaagtaga atcctcggtc cctcgagggc gattagctca gttggttaga gcgcatctct
     1141 gataaggatg aggtccctgg ttcgaatcca ggatcgccca cttgacagga gtcgggcggg
     1201 gtccaacccg ccctccacgt tgaacccgcc ccacccactc gaagcaactg atggctgggc
     1261 ggggctctgc ccgaggtagc gcttcttata atcggtctct catgcgtgaa gtccagatct
     1321 atggcgtctc cttcgacatg gtcggcaagc agccgatcgt cctcctgaag acgatcgacg
     1381 acaatcgcta tctgccgatc tg
//
LOCUS       DAOTIP010000627         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_111271_length_1398_cov_3.181683, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000627 DAOTIP010000000
VERSION     DAOTIP010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111271_length_1398_cov_3.181683"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="P5138_10570"
     CDS             <1..630
                     /locus_tag="P5138_10570"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRV61064.1"
                     /translation="EAFAEKVALPAVGEALAPRIAVRARPISKAPVRAVMKAFRFDYR
                     PTVFFAIDRRETGQGEWLKVRIPGRPNGRTGWVRGSRIRLLRSAGPFRIVIDRSQRRL
                     RLYRHKRMVMKTKVAVGTPDAPTPLGHFYLTAGFVPGDDFLGPWAFETSAYAAITDWP
                     RGGIVGLHGTSETWSVGLRASHGCLRVYNKVILALKKRVRPGTPLRIRA"
     gene            671..1132
                     /gene="ispF"
                     /locus_tag="P5138_10575"
     CDS             671..1132
                     /gene="ispF"
                     /locus_tag="P5138_10575"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRV61065.1"
                     /translation="MAISVGIGYDSHRFADDRRLILGGVEVPHEMGLSGHSDADVLTH
                     AIIDAVLGAAGLGDIGEVFPDTDPAWEGADSISLLSTVIGMLRGPITNVDATLICEQP
                     KLGPHRPAMVARLSEITGSRVSVKATTNEQMGWIGRGEGIACMAVAAVNLD"
     gene            1156..>1398
                     /locus_tag="P5138_10580"
     CDS             1156..>1398
                     /locus_tag="P5138_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61066.1"
                     /translation="MNPVKSAAQAVANKAFEIRTLHKAGVVGGLGPAALPVAASALIR
                     GGTTPATAIRVAAVKNGDRVYLEDERGSLTFAEVHRR"
BASE COUNT          234 a          462 c          467 g          235 t
ORIGIN      
        1 gaagccttcg cggagaaggt ggcgctaccg gcggtcggtg aggcgctagc cccccggatt
       61 gcggtgcggg cgagaccgat atcgaaggcc ccggtcaggg cggtgatgaa ggcgtttcgt
      121 ttcgactacc ggcccaccgt cttcttcgcg attgaccggc gggaaaccgg ccagggcgag
      181 tggctcaagg tcaggatccc cggccgcccg aacggtcgca ccggctgggt caggggttcg
      241 cggattcgtc tgctgcggag cgccgggcca ttccggatcg tgatcgaccg cagtcagcgg
      301 cgcctgcggc tctaccgcca caaacgaatg gtcatgaaaa cgaaggttgc ggttggcacc
      361 cccgacgcac cgaccccgct cggccacttc tacctgaccg cgggtttcgt ccccggtgat
      421 gactttctcg gcccctgggc cttcgagacc agcgcctatg cggcaattac cgactggccg
      481 cgaggcggga tcgtcgggct gcacggcacc agtgaaacct ggtcggtcgg ccttcgcgcc
      541 agccacggct gcctgcgggt ctacaacaag gtgatcctcg ccctgaagaa gcgggtccgg
      601 ccgggaaccc cgctgcgcat ccgcgcatga gctgccccgt cgactgcgga tcctgcgact
      661 atccccgctg atggcaatca gcgtcggcat cggatacgac tcccaccgct tcgcggatga
      721 ccgcaggctg atcctcggcg gggtggaggt tcctcacgag atgggcctga gcggtcactc
      781 ggacgccgac gttctgaccc acgcgatcat tgacgctgtc ctcggtgccg ccgggctcgg
      841 tgacatcggc gaggtcttcc ctgataccga tccggcctgg gaaggcgccg actcgatcag
      901 cctgctcagc acggtaatcg ggatgctgcg ggggccgatc acaaacgtcg atgccacctt
      961 gatctgcgag cagccaaagc tgggccccca ccgtccggcc atggtcgcaa ggttgtctga
     1021 aataacgggg agccgggtaa gcgtcaaggc aaccacgaat gagcagatgg ggtggatcgg
     1081 ccggggcgaa gggatcgcct gcatggctgt tgccgccgtc aatctcgact gatcctcaag
     1141 tagagtcatt cctcgatgaa cccggtcaag tcggccgccc aggccgtcgc caacaaggca
     1201 tttgaaatcc gcaccctgca caaggccggg gtggtcggcg ggctcggccc ggctgcgctc
     1261 ccggttgctg cctcggccct gatccggggc gggacgactc ccgcgaccgc gattcgcgtc
     1321 gccgccgtca agaacgggga ccgggtctat ctggaggatg aacgcggctc cctgactttc
     1381 gccgaagttc accggcga
//
LOCUS       DAOTIP010000628         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_111272_length_1398_cov_3.151154, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000628 DAOTIP010000000
VERSION     DAOTIP010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111272_length_1398_cov_3.151154"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1398)
                     /locus_tag="P5138_10585"
     CDS             complement(<1..>1398)
                     /locus_tag="P5138_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV61067.1"
                     /translation="EAPPENFDVPGTESQQAYDLFEEHSPALAGVDATIVFDATEGTV
                     EQPSTKQAIRESLGEIKQQPDVLTASDPFAEKSGAVSKDGKIAYVDVRYDLDFGDITK
                     VQSERLEDATDPVKSDGVEVAMRGIVIDAGQQEQAPVGELIGVIIAIFLLTFLFRSFA
                     AMIATLLGALIGVMVGQMLLVILAAPLGLPDFAATIAMMLGLGAGIDYSLLILGRYRE
                     QAAAGDSTRDAAAKSAATSGSAVLAAGLIVMVAIAGLLVIGIPLIGKMGIGAAIGVAA
                     VVVSALTFLPIMIGALQRWLKPKKLKHVLPSPAFGRWAERITGKPWIAIAVGVLILLV
                     FASPLLDMRLGSPDDGNQPEGKTQRVAYDLVSEGFGKGVNGPFLIAVDTPDGADQSKA
                     ELNAIVDDLRKVDGVANVSPATPSEDNQMATIFLIPTTSPQDSATSDLLAELRTTVLP
                     DATQGTGLNAYVGGNT"
BASE COUNT          228 a          432 c          482 g          256 t
ORIGIN      
        1 ggtgttgccg ccgacgtagg cattcaggcc ggttccctgc gtggcgtccg ggaggacggt
       61 ggttctgagc tccgcgagca ggtcgctggt cgccgaatcc tggggcgagg tggtgggaat
      121 caggaagatc gtcgccatct ggttgtcctc gctgggcgtg gccggtgaca cgtttgccac
      181 gccgtcgacc ttgcgcaggt catcgacgat ggcgttcagt tcggccttgc tctgatcggc
      241 cccgtcgggg gtgtcgacgg cgatcaggaa gggtccgttc acgcccttgc cgaaaccttc
      301 gctgaccagg tcataggcga cccgctgtgt cttgccttca ggctggtttc cgtcatcggg
      361 cgagccgagg cgcatgtcga gcagggggga ggcgaagacg agcaggatga gcaccccgac
      421 ggcgattgcg atccagggct tgccggtgat ccgctcggcc cagcggccga aggccggcga
      481 cggcaggacg tgtttgagct tcttgggttt cagccagcgc tggagtgcgc cgatcatgat
      541 cggcaggaag gtcagagccg atacgacgac cgcggcgact ccgattgccg cgccaatccc
      601 catcttgccg atcagcggga tgccgatcac gaggagcccg gcgatcgcga ccatcacgat
      661 caggccggcc gccagaacgg ccgacccgga ggtggcggcc gatttcgccg cggcatcacg
      721 ggtcgagtct ccggccgctg cctgctccct gtagcggccg aggatcaaca gcgagtagtc
      781 gatcccggcg ccaaggccga gcatcatcgc gatggttgcg gcaaagtcag gcaggccgag
      841 cggggcggcg aggatgacca gcagcatctg accgaccatc acgccgatga gggcgccgag
      901 cagggtggcg atcatggccg cgaatgaacg gaagaggaag gtgagcagga agatcgcgat
      961 gatcacgccg atgagttcgc cgaccggtgc ctgctcctgc tggccggcat cgatcacgat
     1021 gccgcgcatc gccacttcca cgccgtcact cttgaccggg tcggtggcgt cctcgagtcg
     1081 ttccgactgg accttcgtta tgtccccgaa gtcgaggtcg taccgaacgt ctacataggc
     1141 gatctttccg tctttcgaga ccgctcccga cttctcggcg aacggatcac tggccgtgag
     1201 cacatccggc tgctgcttga tctcgccaag tgattccctg atcgcctgct tcgtcgaagg
     1261 ctgctcgacg gttccctcgg ttgcgtcgaa gacgattgtg gcgtcgacgc cggccagggc
     1321 ggggctgtgc tcctcaaaga ggtcataggc ctgctggctc tcggtgccgg gaacatcgaa
     1381 gttctcgggc ggtgcctc
//
LOCUS       DAOTIP010000629         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_111410_length_1397_cov_2.510432, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000629 DAOTIP010000000
VERSION     DAOTIP010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111410_length_1397_cov_2.510432"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="P5138_10590"
     CDS             complement(<1..186)
                     /locus_tag="P5138_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61068.1"
                     /translation="MILPADAGASSGAAGADSAGWIVGGVPGRLTARIASQAGATAIN
                     RKLGSYRIARDEARQLAD"
     gene            487..1146
                     /locus_tag="P5138_10595"
     CDS             487..1146
                     /locus_tag="P5138_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRV61069.1"
                     /translation="MAYPEAVLFDNDGLLLDTEAVWSRAEQKLFQVRGMQFTIDHKRS
                     LVGSSAEIAGRKLARFLNEPGREAELMDELNDLVLAELEGGVDAMVGARELIEILRLA
                     GVPIALVSNSPRRFIERSHEVAGTGMPFEAVVSGHEVPEAKPAPDAYLEACSQLGVEP
                     GRRVIVLEDSPSGVTAAVAAGLYVIGIPSVPGVELSQADLIASSLESESVLHALGVES
                     R"
     gene            complement(1205..>1397)
                     /locus_tag="P5138_10600"
     CDS             complement(1205..>1397)
                     /locus_tag="P5138_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61070.1"
                     /translation="LEFALEDEGHEKDLDRLESMLADLGEISGRAEQGDSGPGDLERV
                     EAALEVAERVIRRRRILEQ"
BASE COUNT          225 a          478 c          460 g          234 t
ORIGIN      
        1 gtcggcgagt tgcctggcct cgtcccgggc gatccggtag ctgccgagct tgcgattgat
       61 tgctgtcgcc ccggcctggc tggcgatcct ggcggtgagt cgtcccggaa ctcccccgac
      121 gatccaaccc gcgctgtccg cgccggccgc accggagctc gctccggcat cggcaggaag
      181 gatcatcggc ggggcctgcg ggtcagtcgt cccggttgcc cctgctgcgc ccggaagcga
      241 gggcatcccg gcgcagatca tgaagaaggc cagaccagat gcgaagagcg caagcagcga
      301 ccggaacgcc ggtgaatcac cagctccctc cgccggctgg attggccggc gttccccagt
      361 ctcgacttcg aaatcgtgca tgcctgtctg taacccgaaa atcgcctcgt ggtatcggat
      421 caaacctgaa atattccaga ccgggccggc gaatcgctgc ctcctcccgg tgaataggct
      481 cccctcatgg cttatcccga agcggtgctc ttcgacaacg acggcctcct gctcgatacc
      541 gaagcggtct ggagccgtgc cgaacagaag ctcttccagg tccgcggcat gcagttcacg
      601 atcgaccaca agcggagcct ggtcggttct tcggcggaaa tcgccgggcg caaactggcc
      661 cgcttcctga acgagcccgg ccgcgaagcc gagctcatgg acgagctgaa tgatctcgtc
      721 ctggccgagc tcgagggcgg agttgacgcg atggtcgggg cacgggagct gatcgagatc
      781 ctgcggctgg ccggagtacc gatcgccctg gtctcgaact cgccccggcg cttcatcgag
      841 cgttcccatg aggtcgccgg aaccgggatg cccttcgagg cggtggtcag cggacacgag
      901 gtcccggagg cgaagccggc tcccgatgcc tacctcgaag cctgcagcca gctcggggtc
      961 gaaccgggca ggcgggtgat cgtgctcgag gattccccct caggggtgac ggcggcggtt
     1021 gcggccggcc tctacgtgat cggcatcccc tcggttcccg gcgtggagct gtcgcaggcg
     1081 gacctgatcg cctccagcct ggaatcggag tcggtcctgc acgcgctggg cgtcgaatcg
     1141 cgctgacctg cccggggacc gcggcagaga ccgggaccgg ggcaggcgaa tacctctccg
     1201 ggagctactg ctcgagaatc cgccgtcggc ggatcacccg ctcggccacc tcgagcgccg
     1261 cctcgacccg ttccagatca ccgggccctg aatctccctg ctcggcccgg ccggaaattt
     1321 cgcccaggtc ggcgagcatc gattcgagac ggtcaagatc cttctcgtgc ccttcgtcct
     1381 cgagggcgaa ctcgagt
//
LOCUS       DAOTIP010000630         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_111485_length_1396_cov_4.193885, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000630 DAOTIP010000000
VERSION     DAOTIP010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111485_length_1396_cov_4.193885"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>1396)
                     /locus_tag="P5138_10605"
     CDS             complement(176..>1396)
                     /locus_tag="P5138_10605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HRV61071.1"
                     /translation="PPHSVTSLTGTPEGCGGAIKSGALTPNQLSTAYDVDELHSRGLD
                     GSGVRVNTLSSFVVGTSDFKTWANCFNLPAPRVNQFAMPGGVADTQDDPDETVLDVEA
                     LAALAPGLKQITPIFVPLDQGFSNSFILFMLGALDPGRQDGTLPDVLSISDGVCEYRF
                     NKSEIYLSNRLLAEAAVLGVTALSASGDLGFQGCETSKPGTSFPASSPYITAVGGTNL
                     SLNAGNGIVSQPVWSTYATEPQQGVGSGGGPSHFWSRPGYQTGPGITPSIQSGNPMRL
                     VPDLGAMASFKPGVAVFQTSTGGWGGGGGTSAATPITAAMVALAVQQEKQAGRPPLGL
                     ITPLLYRLGRGPGYGTTFYDVTVGTSSRKPNSAAGKTPAGGAAQPGYDLATGLGSLKA
                     TAFADAVAANPPAP"
BASE COUNT          230 a          441 c          499 g          226 t
ORIGIN      
        1 tcgccctgga agaacctcga gaccacaatc ccggtcgaga agttcggaaa gatggtgatg
       61 gtccaggcga tcgactacaa gaacaaggtg atcggcaact ccaagctgac accactcggg
      121 aagcaggccc gctagccggt ttcccttagc ggctacccgc cggttgccct catgctcagg
      181 gcgccggcgg gttcgccgcg accgcatccg cgaaggccgt ggccttcagg gagccgagcc
      241 cggttgcgag gtcgtatccg ggctgggcgg ccccgccggc cggggtcttg ccggccgcgc
      301 tgttcggctt cctcgaggag gtgccgaccg ttacgtcgta gaaggtcgtc ccgtaaccag
      361 ggcctcgccc aagccggtag aggagcgggg tgatcaaccc aagcggcggc ctgcccgcct
      421 gcttttcctg ctgcaccgcc agggcgacca tcgcggcggt tatcggcgtc gcggcgctgg
      481 ttccgccgcc gccaccccag ccgccggtgg acgtctggaa caccgccact cccggcttga
      541 aggaagccat cgcgcccagg tccggaacca gtcgcatcgg gtttccggac tggatcgagg
      601 gggtgattcc gggcccggtc tggtagccgg gacgggacca gaagtggctc gggcccccgc
      661 cgctcccgac cccctgctgg ggctcggtcg cgtaggtcga ccagaccggc tgggaaacga
      721 tgccgttgcc ggcgttgagg ctcaggtttg taccgccgac cgcggtgatg tagggagagc
      781 tggccggaaa cgaggtgccg ggcttgctcg tctcacagcc ctggaagccg agatcgccgg
      841 aagccgaaag ggcagtgact ccgagtaccg cggcttccgc cagcagccgg ttcgagaggt
      901 atatctcgga cttgttgaac cggtattcgc agacaccgtc gctgatcgaa agcacgtcag
      961 gcagcgtgcc gtcctgcctg ccggggtcga gggcgcccag catgaacagg atgaacgagt
     1021 tgctgaagcc ctggtccagc ggaacgaaga tcggcgtgat ctgtttcagg cccggggcga
     1081 gagcggcgag ggcctcaacg tcaaggaccg tctcgtcagg gtcgtcctgg gtgtcggcca
     1141 cgccgcccgg catcgcgaac tggttgaccc tgggggcggg gaggttgaag cagttggccc
     1201 aggtcttgaa gtcactggtg ccgacgacga aggagctgag cgtgttgacc cttaccccgg
     1261 aaccgtcgag tccccggctg tggagctcgt ccacgtcgta ggcggtggaa agctggttcg
     1321 gggtcagggc gccggacttg atcgcgccgc cgcagccttc cggagtgccg gtgagggaag
     1381 tcaccgaatg gggcgg
//
LOCUS       DAOTIP010000631         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_111522_length_1396_cov_2.921700, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000631 DAOTIP010000000
VERSION     DAOTIP010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111522_length_1396_cov_2.921700"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="P5138_10610"
     CDS             <1..380
                     /locus_tag="P5138_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HRV61072.1"
                     /translation="QWILDETGGYGADFTFEATGLVNVMQQAVESARMAWGLCTIAGV
                     AGKGETLDIIPRWLITGRRVAGSSFGGVKGRDQVPELVDRYLAGEIDVEPFLSHRITL
                     DEVNKGFDLMEEQDGIRSVIEFN"
     gene            424..1173
                     /locus_tag="P5138_10615"
     CDS             424..1173
                     /locus_tag="P5138_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV61073.1"
                     /translation="MIIQRVEQSEWLSNSYLVCDGEGGTGVLIDGNGVIEPLLERVDR
                     EGITLTHILLTHEHWDHVVDLREVADRYGVPIVASQKTADLVDFKVDEIVGDGDETVS
                     GGLTIKWIATPGHSDGHMALLINGTDVITADVIFKGTVGGTVAPGETGFPELKSSIMD
                     RLMTLPPETRIHPGHREPSTVGEEWENNPFIRVWRGLDEEGSEPCEVNNFGSATLVLW
                     APDYDGTNKAWIRLPDGEDKITGGSQILSRG"
     gene            complement(1183..>1396)
                     /locus_tag="P5138_10620"
     CDS             complement(1183..>1396)
                     /locus_tag="P5138_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61074.1"
                     /translation="SLSIWVYCPTGKTVVSGGFAGGPGTFQTYQSYPTSSDLSGEPLD
                     TWWMSFYNASNANLNGNPKVYALCQG"
BASE COUNT          260 a          470 c          446 g          220 t
ORIGIN      
        1 tccagtggat cctcgacgaa accggcggct acggcgccga cttcaccttc gaggcaaccg
       61 gtctggtcaa cgtgatgcag caggcggtcg agtcggcccg catggcctgg ggcctctgca
      121 cgatcgccgg ggtggccggc aagggcgaga cgctggacat catcccccgc tggctgatca
      181 ccggccgtcg cgtcgcgggc tcctccttcg gcggagtcaa gggccgcgat caggtgccgg
      241 agctggtcga ccgctacctt gccggtgaga tcgacgtgga gccatttctc tcccaccgga
      301 taaccctcga cgaggtgaac aagggcttcg acctgatgga agagcaggac ggcatccgca
      361 gcgtcatcga gttcaattag tgccccgcat caaccacccc gccaaacccc gaaaggatca
      421 cccatgatca tccagcgcgt cgagcagtcc gagtggctct ccaactccta cctggtctgc
      481 gacggagagg gcgggaccgg tgtcctgatc gacggcaacg gggtcatcga gccgctgctg
      541 gagcgggtcg atcgcgaagg catcacactc acccacatcc tgctcaccca cgagcactgg
      601 gaccacgtgg tcgacctccg cgaggtcgcg gaccgctacg gggtgccgat cgtggccagc
      661 cagaagaccg cggacctggt cgatttcaag gtcgacgaga tcgtgggcga cggcgacgag
      721 accgtctccg gcggcctgac catcaagtgg atcgccacgc ccggacactc cgacggccac
      781 atggccctgc tgatcaacgg caccgacgtg atcaccgccg acgtgatctt caagggcacc
      841 gtcggcggca cggtcgcccc cggcgagacg ggtttcccgg agctgaagag ctcgatcatg
      901 gaccggctga tgaccctgcc gccggagacc cgcatccacc ccggccaccg cgagccctcg
      961 acggtcggcg aggaatggga gaacaacccg ttcatccggg tctggcgcgg cctcgacgag
     1021 gagggaagcg agccctgtga ggtgaacaac ttcggctcgg ccaccctggt cctctgggct
     1081 cccgactacg acgggaccaa caaggcctgg atccgcctgc cggacggaga ggacaagatc
     1141 accggcggct cgcagatcct cagccgcggc tgatcttcct tctcacccct ggcaaagggc
     1201 gtacaccttc gggttgccgt tcaggttcgc gttggacgcg ttgtagaacg acatccacca
     1261 ggtgtccagg ggttctccgc tcaggtcgct gctcgtgggg tatgactgat aggtctggaa
     1321 ggttcccgga cccccggcga aaccgcccga aaccactgtc tttccggtcg gacagtagac
     1381 ccagatcgaa aggctg
//
LOCUS       DAOTIP010000632         1394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_111747_length_1394_cov_3.183719, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000632 DAOTIP010000000
VERSION     DAOTIP010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111747_length_1394_cov_3.183719"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1394)
                     /gene="murJ"
                     /locus_tag="P5138_10625"
     CDS             complement(<1..>1394)
                     /gene="murJ"
                     /locus_tag="P5138_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRV61075.1"
                     /translation="ERIASSAARAAEEAIRSTSERAETVINRPAPGSGPAARSVPEAV
                     IDREEAMVETATGLAEEGGVAAPVPVTEGVREEPGPRQSAKPEKAGRLARSTAFFSIA
                     TAASRVAGLVREVVAASYFGISGPMSAFTVAFQVPNLIRALFADAALQPAFVPIFTEQ
                     IEKGKYREAFRMASTLLLLVTMILGAITAFFILAAGLVMPIFAPGFSAATESLMIVLS
                     QILFPILILLGVTGIVVGILNSYDRFGAFAISPFFWNLTIIAVVIAGVPLFPEDKQIN
                     AYAIGVVAGTVVQLLIPTFDLRNTPFRFTLKVDFKDPNVRRVLIMMIPVTLSLGLINF
                     NLLVNSFFGSLVNDEAPAAIDKAFRIYQLPQGIFSVAIATVLFPTLARFANRKDFDSL
                     RTTLAKGMRQIIFVLIPASAAILVLSEPMIRLVYQRGEFDAAETQVVATALFWFAFSL
                     PTNGMYLLLSRTFF"
BASE COUNT          280 a          422 c          476 g          216 t
ORIGIN      
        1 gaagaaggtg cggctgagga gcaggtacat gccgttggtc ggcaacgaga aggcgaacca
       61 aaagagcgcc gtcgcgacga cctgggtttc ggcggcgtca aattcacccc gttggtagac
      121 caggcggatc atcggctcgg aaagaacaag gatcgcggcg ctggccggga tcaggacgaa
      181 gatgatctgc cgcatgccct tggccagggt ggtccgcagg gaatcgaagt ccttgcgatt
      241 ggcgaagcgg gccagggtcg ggaagagaac cgtggcgatg gccacggaga agatcccctg
      301 cggcaactgg tagatccgga acgccttgtc gattgcggcc ggggcctcgt cattgaccag
      361 cgagccgaag aagctgttga cgagcaggtt gaagttgatc aacccgagac tgagggtgac
      421 cgggatcatc atgatcagca ccctcctcac gttcgggtcc ttgaaatcga ccttgagggt
      481 gaaccggaaa ggcgtgttcc tgaggtcgaa ggtggggatc agcaactgga ccacggtgcc
      541 cgccacgacg ccaatcgcgt acgcgttgat ctgcttgtcc tcgggaaaca gcggcacccc
      601 ggcgattacc accgcgatga tcgtgaggtt ccagaagaac ggcgaaatcg cgaaggcgcc
      661 gaagcggtcg tagctgttga ggatgccgac cacgattccc gtcaccccga gcagaatcag
      721 gatcgggaag aggatctgcg agagcacgat catcaggctc tcggtggcag ccgagaagcc
      781 aggcgcgaaa atcggcatga ccagcccggc cgccaggatg aagaaggcgg tgatcgcgcc
      841 gaggatcatg gtgaccagca gcagcagggt cgaagccatg cggaaggcct cgcggtactt
      901 ccccttctcg atctgctcgg tgaagatcgg tacgaaggcc ggctgaagcg ccgcgtcggc
      961 gaaaagggcg cggatcaggt tcgggacctg gaaggcgacg gtgaaggccg acatcgggcc
     1021 cgagatcccg aagtagctcg cggcgacgac ctcccggacc agcccggcca ccctcgatgc
     1081 ggcggtggcg atggagaaaa aagccgttga cctggccagc cggcccgcct tctccggctt
     1141 tgccgactgc cgcgggcccg gctcttccct gacgccctcg gtcaccggga ccggcgcggc
     1201 gacccctccc tcctcggcca gtccggtcgc cgtctcgacc attgcttctt cccggtcgat
     1261 aaccgcctcc ggaaccgatc gcgcagccgg tccggatccg ggggcaggcc tgttgatgac
     1321 cgtctcggcc cgttcggagg tcgaacggat tgcctcttcg gccgcccggg cggccgaaga
     1381 ggcaatccgt tcga
//
LOCUS       DAOTIP010000633         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_112405_length_1389_cov_2.077211, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000633 DAOTIP010000000
VERSION     DAOTIP010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112405_length_1389_cov_2.077211"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..440)
                     /locus_tag="P5138_10630"
     CDS             complement(156..440)
                     /locus_tag="P5138_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61076.1"
                     /translation="MSQITKPTETIQLPAPSWAPAVFALGVLGLIAGTFANDFMFPAW
                     FYAFVGFFFCLFSLRSMIKKGRRAFYSLPREQGDARAELPVESFTAPTSD"
     gene            complement(594..>1389)
                     /locus_tag="P5138_10635"
     CDS             complement(594..>1389)
                     /locus_tag="P5138_10635"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRV61077.1"
                     /translation="VATMFVADPSGPALSTILWTMLGGYMAAGGAGAINHYIDRNRDA
                     RNNRTKDRPLADGRIKPIHGLIFGIALGAISFFQLWLTVNFLAAALAMVGLFGYVFLY
                     TLWLKPLTPQNIVIGGAAGAVPPLVGWAAASGGLSWEAMWPFLIIFFWTPPHFWALSL
                     MIKDDYAKTGVPMLPVVKGEEATRQAILIYTVFMVGFTVGPYCTGLLGPLYLAASIVL
                     GLVYIGFSFALWRKPGPKITLQAYLYSLAYLALLFISMAVAAVAAA"
BASE COUNT          262 a          380 c          539 g          208 t
ORIGIN      
        1 ccgtctagcc tcgggcggcg ctgacgctgg ttgggcccgc cgcggttcgc agacctgggg
       61 ataggccgcg gggccagcag ggcgctcatc ggttacatcg cgtgcagagg gggccgcacc
      121 gggtccctga cggttccggg gtggtgcccg ggggcttagt cagaagtggg ggcggtgaag
      181 ctttctactg gcagctccgc gcgggcgtcg ccctgttcgc gggggaggct gtagaaggcc
      241 cgacggccct tcttgatcat gctgcgcagc gagaagaggc agaagaagaa accgacgaag
      301 gcgtagaacc aggccgggaa catgaagtcg ttggcgaagg ttccggcgat caggccgagc
      361 acgccgaggg cgaacactgc cggtgcccag gagggggcgg gaagctggat ggtctcggtc
      421 ggcttggtga tctggctcat ctcgggttcc tagttcgcga tgtagaggac cgcgaagacg
      481 aaggtcatgg cgaagatcgc cgtggaaccg gcgccgagaa gcatgccggt ggtgaaattc
      541 gacttggaag tgttgcgatc catgagcgga gttttatcag ccggaggggg acgtcaggct
      601 gctgccacag cagcgactgc catcgagatg aagagcaggg cgaggtaggc gagcgagtac
      661 aggtaggcct ggagggtgat cttcgggccg ggcttgcgcc acagggcgaa ggagaagccg
      721 atgtagacca ggccgaggac gatcgatgcg gccaggtaca gcggaccgag cagcccggtg
      781 cagtaggggc cgaccgtgaa gccgaccatg aagacggtgt agatgaggat cgcctggcgg
      841 gtcgcttcct cgcccttgac caccggcagc atcggcaccc cggtcttggc gtagtcgtcc
      901 ttgatcatca gggagagcgc ccagaagtgg ggcggggtcc agaagaagat gatcaggaag
      961 ggccacatcg cctcccagct cagcccgccc gaagcagcgg cccagccgac cagcggcggc
     1021 acggcgccgg cggcgccgcc gatcacgatg ttctgcggag tcagcggctt gagccagagg
     1081 gtgtagagga agacgtatcc gaagaggccg accatggcca gggcggcggc caggaagttg
     1141 acggtgagcc agagctggaa gaaggagatg gctccgaggg cgatcccgaa gatcaggccg
     1201 tggattggct tgatccggcc gtcggcgagg ggacggtcct tggtccggtt gttgcgggcg
     1261 tcgcggttcc ggtcgatgta atggttgatc gctccggcac cgccggcggc catgtacccg
     1321 ccgagcatgg tccagaggat ggtggagagg gccggaccgg acggatcggc gacgaacatc
     1381 gtcgccacc
//
LOCUS       DAOTIP010000634         1386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_112703_length_1386_cov_2.494365, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000634 DAOTIP010000000
VERSION     DAOTIP010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112703_length_1386_cov_2.494365"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="P5138_10640"
     CDS             <1..351
                     /locus_tag="P5138_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV61078.1"
                     /translation="RVSTKGGKMKLTLKTLLGSAALTGLAVLAPFAAQAETYKVAILQ
                     ALTGPAGFLGAPFAEGIQMAFDHANETGGFGEGITIEYSVADDASDKSQVITLMRRYA
                     NDPKILIVAGPTRP"
     gene            351..>1386
                     /locus_tag="P5138_10645"
     CDS             351..>1386
                     /locus_tag="P5138_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61079.1"
                     /translation="MLSILAGALCLAAWRALSNVEPDQAFSIIKSLAPVAIGSIGLAS
                     ALATQRFLSTRRALGSRRAVAVVPADEFEVSIETVLRAAAQLSRSDRAVRGWMCRRAS
                     AIRFTLDSDPEGRVVYLIESREDQMDLVRSTLRAYEGIEIRRADEVRPARQAGTGVTM
                     RTELVLARPSVEPLARLELDPDPLQPFAAAMAGLQPGEHASVHIDLLPAAGHRRARLT
                     RHLARQVRRLGIDQVGISERLGLNSKPRRSDPATLAERRGLGRGLDAKLRDGGAIFEA
                     QILIRTRAESRVRASATMTNLLAAFESLGDRNWLRASGVPIPGLAFLGSDLPGRRRRF
                     DRRVNTGLFRP"
BASE COUNT          221 a          470 c          443 g          252 t
ORIGIN      
        1 agggtttcca ccaagggagg taagatgaaa ctcacattaa agacgctgct cggctcagcc
       61 gctctcacgg gactggcggt tctcgcgccc tttgcggccc aggccgagac ctacaaggtc
      121 gccatccttc aggctctgac cggtccagcc ggctttctcg gggctccctt tgcggagggc
      181 atccagatgg ccttcgatca cgccaacgag acaggcggct tcggcgaggg catcacgatc
      241 gagtatagcg tcgcggacga cgcctcggac aaatcccagg tgatcacgtt gatgcgccgc
      301 tatgcgaacg atccgaagat cctgatcgtc gccggtccga cccgaccctg atgctctcca
      361 tcctcgcggg tgctctctgc cttgccgcgt ggcgggcact ctcgaacgtc gagccggacc
      421 aggctttctc gatcatcaag tcgctggctc cggtcgctat cggatctatc ggtctcgcct
      481 cggcgctggc tacacaacgc ttcctatcta cccgccgcgc tctcggctcc cgccgggccg
      541 tggcggtggt gccagccgac gagttcgagg tcagcatcga gaccgttctc cgggctgctg
      601 ctcaactctc gcgatcagat cgtgccgtgc gtggctggat gtgccgcaga gcgtcggcga
      661 tcaggttcac cctcgactct gacccggagg gaagggtcgt ctacctgatc gaatcccgcg
      721 aggatcagat ggacctggtc cggtcaaccc ttcgggccta cgaaggaatc gagattcgtc
      781 gggccgatga agtgcggccc gcgcggcagg ccggtacggg ggtgacgatg cggaccgagt
      841 tggtgctggc ccgcccgagc gtggagccct tggcgcgcct ggagctggac cccgatccgc
      901 tccaaccatt tgcggcggcg atggccggtc ttcagcccgg cgagcatgcg agcgtccaca
      961 tcgatctact tcccgcggcc ggtcaccgtc gggccaggct gacacgacac ctcgcccgac
     1021 aggtgcgtcg cctcggcatc gaccaggtgg ggatctctga gcgtctcggg ttgaactcga
     1081 agccgaggcg aagcgacccg gcgacgttgg ccgagcgacg agggctcggc cgcggcctcg
     1141 atgccaagct ccgagatggc ggagcgatct tcgaagccca gatcctgatc cgtacccggg
     1201 ccgagagccg ggttcgtgcg agcgcaacta tgacgaacct gctcgccgcg ttcgaatctc
     1261 tcggcgacag aaactggctc agagcctccg gtgtcccgat ccctggtcta gcgttccttg
     1321 gctccgatct tccgggccgt cgcaggcgct tcgaccggcg cgtcaacacc ggcctcttcc
     1381 ggcccg
//
LOCUS       DAOTIP010000635         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_112793_length_1385_cov_3.015038, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000635 DAOTIP010000000
VERSION     DAOTIP010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112793_length_1385_cov_3.015038"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="P5138_10650"
     CDS             complement(<1..184)
                     /locus_tag="P5138_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61080.1"
                     /translation="MNTEPAAPKMNGKRRMLIIVNPYATTVSDRLKNLVVYALQGRYE
                     VEVEATESQDHATEIGR"
     gene            complement(181..1200)
                     /locus_tag="P5138_10655"
     CDS             complement(181..1200)
                     /locus_tag="P5138_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV61081.1"
                     /translation="MAEPEPTPESEAALARASEWGIHTIPAPTPFAVGRVNCYLIEGD
                     PLTLIDAGPRSEKSWEGLKSGVEAAGYGIEDIDLVVATHQHIDHIGGISSVVEVSGAD
                     VAAIDISVDRLAKFSWGSEKEDQMAVDLMVRYGVKEDVANVLKEVTASFRELGAPVEV
                     TRPFSAGSKITMGSREYEIFHRPGHSPSDTVFWDRDRQLMFGGDHLLSHISSNPLISQ
                     SLDGSGERTKSLINYRDSLKATAAMPVEIMLSGHGLAITDHADLIRRRLAGQDRRTEK
                     IYDLVAEGHSSPHGVASAIWGDIALTQAYLTLSEVIGHLDILISDGRVVEHEGERYST
                     FEAVG"
     gene            complement(1193..>1385)
                     /gene="manB"
                     /locus_tag="P5138_10660"
     CDS             complement(1193..>1385)
                     /gene="manB"
                     /locus_tag="P5138_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578506.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRV61082.1"
                     /translation="EIEAMHPDAEITHLDGVSIDYEDWHFNVRPSNTEPLLRLNLESL
                     VSREDMEQKRDEVLSVIRG"
BASE COUNT          225 a          427 c          441 g          292 t
ORIGIN      
        1 cgcggccgat ctccgttgca tggtcctggc tttcggtggc ctcgacctcg acctcgtagc
       61 gaccctgcag ggcgtagacg acgaggttct tgagacggtc ggaaaccgtg gtcgcgtagg
      121 ggttgacaat gatcagcatg cgccgcttgc cgttcatctt cggtgccgcc ggttccgtat
      181 tcatccgacc gcctcgaagg tcgaatacct ctcgccctca tgctcgacca cccgtccatc
      241 actgatcagg atgtcgagat gtccgatcac ttcggaaagc gtcaggtagg cctgggtcag
      301 ggcgatgtca ccccagattg cgctggcaac cccgtgcggc gagctgtggc cttcggcgac
      361 caggtcgtag atcttctcgg tacggcggtc ctgtccggcc agtcttctcc tgatcaggtc
      421 tgcgtgatcc gtgatcgcga gtccgtggcc ggaaagcatg atctccaccg gcatcgcagc
      481 ggttgccttt aacgaatccc ggtagttgat cagggacttc gtgcgctctc cgctgccgtc
      541 cagggactgc gagatcaggg ggttcgagga gatgtgggag agaaggtgat cgcctccgaa
      601 catcagctgc cggtctctgt cccagaaaac cgtgtcggag gggctgtgtc cgggccgatg
      661 gaagatctcg tattccctgg agcccatggt gatcttcgac ccggcgctga atggccgggt
      721 gacctccacc ggagcaccga gttcgcggaa gctggcggtc acctccttga gcacgttggc
      781 gacgtcttcc ttcacgccgt agcgaaccat caggtccacc gccatctggt cttccttctc
      841 ggagccccag ctgaacttgg ccaggcggtc gacggaaatg tcgatcgcgg cgacgtcggc
      901 gccggaaacc tcgaccaccg agctgatccc gccgatgtga tcgatgtgct gatgcgtggc
      961 gacgacgagg tctatgtcct cgattccgta gccggcggcc tcgactcccg acttcaggcc
     1021 ttcccacgat ttctccgagc gcggtccggc gtcgatcagg gtgagcgggt cgccttcgat
     1081 cagatagcag ttgacccgtc ctaccgcgaa gggggtaggt gccgggatgg tgtggatgcc
     1141 ccattcgctg gcccgggcga gtgccgcctc ggattcgggt gtcggttcgg gctcagccac
     1201 gtatcaccga gagaacttcg tcccgcttct gttccatgtc ctcgcgcgag acgagcgact
     1261 ccaggttcag gcgcagcagc ggctccgtgt tcgagggacg gacattgaaa tgccagtctt
     1321 cgtaatcgat cgagacgccg tcgagatggg tgatctcggc gtcggggtgc attgcctcaa
     1381 tctcg
//
LOCUS       DAOTIP010000636         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_113095_length_1383_cov_2.208584, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000636 DAOTIP010000000
VERSION     DAOTIP010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113095_length_1383_cov_2.208584"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5138_10665"
     CDS             complement(<1..418)
                     /locus_tag="P5138_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV61083.1"
                     /translation="MLPATLAQDIRQQVIHYLEATFNFRRRDDEEALRRFINDPENGL
                     FKGPWVQIRRPFRPAADHGNRFFDLPVPFLPFRHQWLAWRRLTSKGQEPASTLITTGT
                     GSGKTECFQVPILNHCYREHRAGRKGIKAIILYPMNA"
     gene            complement(451..>1383)
                     /locus_tag="P5138_10670"
     CDS             complement(451..>1383)
                     /locus_tag="P5138_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61084.1"
                     /translation="ALKPEILMPGGRQIYEEALGTGLPNIRLTPVRHYGPPGQRTAAP
                     SSVPGELDKTLWFRGTSNATALATRLGARLFEMLESLRTTDERRAPNLRYDAVLLKAL
                     LVHGARWDDAGNRMRGIFKQEIGGRKIKDLVGRFLGYGLVDPARVLGCTERRATLLGW
                     GSLTNAEAHAFALPLPADLSGTRGLRRLTITLAWLSPIAPGHQRYRKAHLWFTTDATE
                     LIGTGRASNAYDHNLVKRGTVQHEMFEGEKARAFVDGEAVSIQINCREDATGLDETVD
                     YGLVATFEVGEEVPVPVYQQIRQRLREAIPIRVG"
BASE COUNT          247 a          415 c          438 g          283 t
ORIGIN      
        1 gcgcgttcat gggatagagg atgatcgcct tgatgccttt ccggccggca cgatgttcgc
       61 gatagcagtg attgagaatc ggcacctgaa aacactcggt tttacccgag ccggtgccgg
      121 tggtgatcag ggttgaggcc ggctcttgtc ctttcgaggt cagccgccgc caggccagcc
      181 attggtgacg gaaggggaga aagggaaccg gcaggtcgaa gaaccggttg ccgtgatccg
      241 cagccgggcg gaagggccgg cgaatctgta cccacggccc cttgaacagg ccgttttccg
      301 ggtcgttgat gaagcgccgc agggcttctt cgtcgtcgcg ccggcgaaaa ttgaacgtgg
      361 cttccagata gtggatgacc tgctggcgaa tgtcctgggc gagggtggcg gggagcatta
      421 gcgttccgtg atgacaaaaa tcgccaggcc ttatccgacc cttatcggga tagcttctcg
      481 tagtcgctgc cggatctgct ggtacactgg aactggcacc tcttcgccga cttcaaacgt
      541 ggccaccaaa ccataatcta ctgtctcatc cagtccggtg gcatcttccc ggcagttgat
      601 ctggatgctt acggcctcac cgtcgacaaa ggcacgggct ttctcgccct cgaacatttc
      661 gtgctggacg gttccgcgct tgaccaggtt atggtcgtaa gcattgctgg ctcggccggt
      721 tccaatcaat tcggttgcat cggtggtgaa ccagaggtgc gccttgcggt agcgctggtg
      781 accgggtgcg attggtgaaa gccaggccag ggtgatggtg agtcggcgta gaccccgggt
      841 accgctcaga tcagccggca acggcagggc gaaggcatgc gcctcggcgt tggtcagact
      901 tccccaaccg agcagggtag cccgccgctc ggtacatccc aggacccgtg ccggatcgac
      961 gagtccgtaa ccaagaaacc gtccaacgag atccttgatc ttgcgtccgc cgatctcctg
     1021 tttgaagatg ccgcgcatcc ggttgcccgc gtcatcccag cgcgcgccgt ggaccagcag
     1081 tgccttgagt aagacggcat cgtaccgtag attcggtgct cgtctttcgt ccgttgtccg
     1141 cagggattcc agcatctcga acagccgtgc cccgagacgg gtagcgagcg ctgtggcgtt
     1201 gctggtccct cggaaccaga gggtcttgtc caactcgcca ggcaccgaac ttggagccgc
     1261 cgtcctctgc ccaggtggcc cgtagtgtcg aaccggggtg agccggatgt tcggcaagcc
     1321 ggtccccaac gcctcctcgt aaatctgccg cccgcccggc atcaggattt cgggcttcag
     1381 cgc
//
LOCUS       DAOTIP010000637         1372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_114362_length_1372_cov_3.087320, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000637 DAOTIP010000000
VERSION     DAOTIP010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114362_length_1372_cov_3.087320"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..616
                     /gene="nrdR"
                     /locus_tag="P5138_10675"
     CDS             44..616
                     /gene="nrdR"
                     /locus_tag="P5138_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRV61085.1"
                     /translation="MGIAWCADPAKRGKCFPAKHYIWCFKKVSDASPSIICHVRCPHC
                     GHQGSRVLESRSSEEGSAVRRRRQCSSCESRFTTYERYESQALFVIKRDGGRQPFDRA
                     KLRGGLERASHKRPVRPEAIDALVDRIEKKAIRDGGEIEAARIGDMCLAGLKRIDQVA
                     YLQFAAVYRQLGVEDVQAELDRLSSPLSRN"
     gene            753..>1372
                     /locus_tag="P5138_10680"
     CDS             753..>1372
                     /locus_tag="P5138_10680"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936882.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRV61086.1"
                     /translation="MSQSVSESTRVNPEGVVVERRLTREGVHPFDEIEWEVRDAVIGD
                     PAKPAFEQRGVEFPKSWSQNATNIVAQKYFRGQIGSPERESSVKQMIGRVAGTIADWG
                     REGGYFASDADADVFEAELTATLLHQKVAFNSPVWFNVGFEETPQCSACFILSVEDTM
                     ESILDWNTKEGKIFRGGSGSGINLSNIRGSMEPLKKGGTASGPVSF"
BASE COUNT          289 a          419 c          434 g          230 t
ORIGIN      
        1 gaatcgtagc ggcccgggga aacggccggg aaaccgagtg ggcatgggca ttgcgtggtg
       61 tgccgatccc gcaaagcgcg gaaagtgttt tcctgcgaaa cactacatat ggtgtttcaa
      121 aaaggtatcc gacgccagtc ctagtataat ttgccatgtg cgttgcccgc attgcggaca
      181 tcaaggaagc cgtgtcctcg aaagccgtag ctccgaggag gggagcgccg tgcgccgaag
      241 gcgccagtgt tccagctgcg aaagccgttt caccacttat gagcggtacg agtcacaggc
      301 cttgttcgtc atcaagcggg atggcgggcg ccagcccttt gatcgcgcca agttgcgcgg
      361 agggctggag cgcgcctcgc acaaacgtcc ggtcagaccc gaagccatcg acgctttggt
      421 cgaccggatc gaaaagaagg ccatccgtga tggcggagag atcgaggcgg cccgcatcgg
      481 cgacatgtgc ctggcgggac tcaagcgaat cgaccaggtc gcgtacctgc agttcgccgc
      541 cgtctaccgc cagctcgggg tcgaggacgt acaggcggaa ctcgaccggc tgagctcgcc
      601 gctcagcaga aactgaactt gtaaagcccc ggaagtaccg caggagacac agcatcacca
      661 gtcaaggcgt cacccagtca ccgtcaagta attcgagcag gaccagcagc aagcagagcc
      721 agtcgtccta gtagcgagga gagagccaaa gtatgtccca gtcagtatcc gagtcgaccc
      781 gcgtcaaccc cgaaggtgta gtcgtcgaga ggcgcctcac ccgggagggt gtccatccct
      841 tcgatgaaat cgaatgggag gtccgtgatg ccgtgatcgg cgatccggcc aagccggcat
      901 tcgagcagcg cggggtcgag ttcccgaaga gctggtcgca gaatgcgacc aacatcgtcg
      961 cccagaagta cttccgcggc cagatcggct cacccgagcg cgagagctcg gtcaagcaga
     1021 tgatcggccg cgtggccggc acgatcgccg actggggccg cgagggcggg tacttcgctt
     1081 ccgatgcgga cgccgacgta ttcgaagccg agctgaccgc gaccctgctc caccagaaag
     1141 tcgccttcaa ctcgccggtc tggttcaacg tcggcttcga ggagactccg cagtgctcgg
     1201 cctgcttcat cctctcggtc gaggacacga tggagtcgat cctcgactgg aacaccaagg
     1261 aaggcaagat cttccgcggc ggttcgggtt ccggcatcaa cctctccaac atccgtggct
     1321 cgatggagcc cctgaagaag ggcggcaccg cttccggccc ggtctccttc at
//
LOCUS       DAOTIP010000638         1370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_114602_length_1370_cov_3.547529, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000638 DAOTIP010000000
VERSION     DAOTIP010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114602_length_1370_cov_3.547529"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5138_10685"
     CDS             complement(<1..210)
                     /locus_tag="P5138_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRV61087.1"
                     /translation="MSLVRTERDGPLTVITLDSPPLNLFDRAMTTELIEALEQVQDDP
                     PRGLLIRAEGKVVSGGVNVQEFKGLT"
     gene            complement(214..912)
                     /locus_tag="P5138_10690"
     CDS             complement(214..912)
                     /locus_tag="P5138_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV61088.1"
                     /translation="MTRLAAGRTTHLEPEEIAAETLRLFDQGDDPSIRQLATALNVTP
                     SAIYHHFDSRAEIVQAAVELVWIEILGNTAAEVGNPFESDPEEVLTAVGLHARRAFIE
                     HHTIAPYMTVIPRSRQLSAGGIALFANAFERLGVTGEAAGDAFHYYASYCFGTALYAA
                     SRINARTALGTESPGHEELEGLRQEAQVDGTSGESTRAALDRVLDLSELDPDRDEELF
                     VLGLKQILQGFGKV"
     gene            complement(1066..>1370)
                     /locus_tag="P5138_10695"
     CDS             complement(1066..>1370)
                     /locus_tag="P5138_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV61089.1"
                     /translation="VRAEDHLVDLALYRSRDLISRLREKTMAPIGELTPKARERMAET
                     ALAYVEHSGNAAEMARSLSIHPQTARYRIARLRELFGEELDRPDLRLALELSLRSR"
BASE COUNT          224 a          431 c          461 g          254 t
ORIGIN      
        1 ggtcagcccc ttgaattcct gcacgttgac cccgccggag acgaccttcc cttcggcccg
       61 gatcagcagt ccgcggggcg ggtcgtcctg gacctgttcc agtgcttcga tgagctcggt
      121 ggtcatcgcg cgatcgaaga gattgagtgg cggggagtcg agcgtgatca cggtcagcgg
      181 gccgtcccgc tctgttctga cgagtgacat gactcagact ttgccgaatc cctggaggat
      241 ctgcttcaag ccaagcacga aaagttcctc atcgcggtcg ggatcaagct ccgagaggtc
      301 caggacccgg tcgagtgccg cccttgtcga ctccccggaa gtcccgtcca cctgggcctc
      361 ctgtcgcagc ccctcgagct cttcgtgacc ggggctttcg gtgcccagcg ccgtcctggc
      421 gttgatgcgg ctggccgcgt aaagagccgt tccgaagcaa tagctggcgt agtagtgaaa
      481 cgcgtccccg gccgcctcgc ccgtgacccc gagccgctcg aaggcgttgg cgaagagggc
      541 gatccctccc gccgagagtt gccgggaacg tggaatgacc gtcatgtagg gagcaatggt
      601 gtgatgctcg atgaaggcgc ggcgcgcgtg aagtccgacc gcggtgagga cttcctcggg
      661 atcggattcg aaggggttgc cgacctccgc cgcagtgttg ccgaggatct cgatccagac
      721 cagctctacc gcggcctgga cgatctcggc cctcgagtcg aagtggtgat agatcgcaga
      781 gggggtgacg ttcagcgcag ttgcgagctg gcggatgctg gggtcatccc cctggtcgaa
      841 cagcctcagg gtctcggccg cgatttcctc gggctcgaga tgggtggttc gccctgctgc
      901 cagtcgggtc aacttcttct ctccttgacc tgtgccccgt ctgctcctct gcccggggac
      961 ctcggaactc attccgcaaa accgaacggc gcacggaatg taacaactga ggcgggaagt
     1021 tcaagacgga tctcgaaaga cccggccggg aaacccccgg ggcgttcagc gggagcggag
     1081 cgagagctcg agggcgagac gcaggtcggg ccggtccagt tcttcgccga aaagctcccg
     1141 cagccgcgcg atccggtacc tggctgtctg ggggtggatg ctcaggctgc gggccatctc
     1201 cgcggcgttg cccgaatgct cgacgtaagc gagagcggtt tccgccatcc gctcgcgcgc
     1261 cttgggggtc agctcgccga tcggggccat ggtcttctcc cgtagccggg agatcaggtc
     1321 ccggctgcgg tagagagcga ggtcaaccag atggtcttcc gccctgacca
//
LOCUS       DAOTIP010000639         1370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_114662_length_1370_cov_2.151331, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000639 DAOTIP010000000
VERSION     DAOTIP010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114662_length_1370_cov_2.151331"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="P5138_10700"
     CDS             complement(<1..295)
                     /locus_tag="P5138_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRV61090.1"
                     /translation="MARIRSQHVCQSCGHVAMAWTGQCPDCREWNTLVEQAAPRPAAK
                     APGGKSGNGRALAAGAAGQVLPLKDVRASRVERFPTGIGEFDRVLGGGLVPGSL"
     gene            420..797
                     /locus_tag="P5138_10705"
     CDS             420..797
                     /locus_tag="P5138_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61091.1"
                     /translation="MFWTVLLFLHVVAMAFFLGGQIMLAFTVVPVMRAEPAQPERIKQ
                     IAQRFGVGSLIALGVLLITGMMMASHFQLWDYGPFQVKMTLVIATIVAVLIHTLRGNS
                     HVLMGITFLLSLATVYAGVNLTH"
     gene            904..1245
                     /locus_tag="P5138_10710"
     CDS             904..1245
                     /locus_tag="P5138_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61092.1"
                     /translation="MSTSEDLSPQAPGGAREIRFLNIIAGVAILDFLLLIPLVWASRW
                     FADKHDIVSVLGPIHGFFFVVLIGLCAYGSLEKWWGWWFPILTLVTGGAIGSLIGDLV
                     VRRQLREKAAA"
BASE COUNT          225 a          466 c          425 g          254 t
ORIGIN      
        1 ccagtgatcc cggaaccagc ccgccgccga ggacccggtc gaactcgccg atcccggtcg
       61 ggaaccgttc gacccgggaa gcccgtacgt ccttcagcgg gagtacctgg ccggcggcgc
      121 cggccgcgag ggcccggccg ttgccggact tgccgccggg ggccttcgct gccggccttg
      181 gcgcggcctg ctcgaccagc gtgttccatt cccggcaatc cgggcattgg ccggtccagg
      241 ccatcgccac gtgaccgcag ctctgacaga catgttgtga acggatcctg gccacctgat
      301 gagagtaagg acgggagagg acgctttcgc cccccgggac aagccgaaac tcagctcaag
      361 gcaacatctg cggcaaatcc gcaggttttc cggcgagacg catcaatatc cttcggacca
      421 tgttctggac cgtcctgttg ttcctccacg tagtcgccat ggctttcttc ctcggcgggc
      481 agatcatgct ggccttcacc gtcgtgccgg tgatgcgggc cgaaccggcc cagcccgagc
      541 ggatcaagca gatcgcgcag cgcttcgggg tcggttccct gatcgcgctc ggcgtgctgc
      601 tgatcaccgg catgatgatg gcctcccatt tccagctctg ggactacggg cctttccagg
      661 tcaagatgac cctggtcatc gccaccatcg tcgcggtcct gatccacacc ctgcgcggca
      721 acagccacgt gctgatggga atcaccttcc tgctgagcct cgcgacggtc tacgccggag
      781 taaacctgac ccactgaatt cccccggacc ggatcgagaa ggccaaagtg agcgctatgt
      841 ggccactttt gccttttcgg tgacaaccgg gcgggatcga cgggttgata gcttcacggg
      901 tccatgagca cctctgaaga cctttcccct caggcacctg gcggtgcccg cgaaatccgc
      961 ttcctcaaca tcatcgccgg ggtcgcgatc ctcgacttcc tgctgctgat cccgctggtc
     1021 tgggcctcgc gctggttcgc cgacaagcac gacatcgtct cggtgctcgg gccgatccac
     1081 ggcttcttct tcgtggtcct gatcgggctc tgcgcctacg ggtcactcga gaagtggtgg
     1141 ggctggtggt tcccgatcct gaccctggtc accggcggag cgatcggctc cctgatcggt
     1201 gacctggtcg ttcggcgcca gctcagggag aaggcggcag catgagcgat tccgacaccg
     1261 ccctggccga agtcacctgg aacctcgacg acctgctcga aggcgaccac gccgacccgg
     1321 aggcagcggt cacggcgctg ctcgacgaag cggagaaggc ttcggagaag
//
LOCUS       DAOTIP010000640         1368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_114866_length_1368_cov_2.757045, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000640 DAOTIP010000000
VERSION     DAOTIP010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114866_length_1368_cov_2.757045"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="P5138_10715"
     CDS             complement(<1..588)
                     /locus_tag="P5138_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61093.1"
                     /translation="MTVSEAPTDPQDPAGNPGTGTPATGLTAAEAARRLEERGPLPKA
                     ETSRSYRSIVVANVFTVFNAILLFFGLLTIFFGNPKDALFMGILVANTTIGIGQEVRA
                     KLALDKLAALIAIKAEVLRDGEKVEVPVEDVVVGDLVLIGAGDQVIADGELLSADALQ
                     LDEANLTGESEPVRREAGGEVWSGSFVVEGEGRFVA"
     gene            734..1003
                     /locus_tag="P5138_10720"
     CDS             734..1003
                     /locus_tag="P5138_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRV61094.1"
                     /translation="MRTTVRLDEQLMRDAKALAAKEGKTFTALIEDSLRERLARSRNR
                     ETREHRSFPVSKMPGGLREGLDPEILSDNSRLADALDEFDGFAGR"
BASE COUNT          251 a          413 c          465 g          239 t
ORIGIN      
        1 cgcgacgaaa cgaccctcgc cctcgaccac gaaagacccg gaccagactt cgccgccggc
       61 ctcgcggcgg accggctccg actcgcccgt caggttcgcc tcgtcgagct gaagggcgtc
      121 ggccgagagg agttcgccgt cggcgatcac ctggtcgcca gccccgatca ggaccagatc
      181 gccgaccacc acgtcctcga ccgggacctc gaccttctcg ccgtcgcgca gtacctccgc
      241 tttgatcgca atcagcgccg ccagcttgtc gagggcgagc ttggcccgga cctcctggcc
      301 gatgccgatc gtggtgttgg cgaccagaat gcccatgaag agggcgtcct tgggattgcc
      361 gaaaaagatc gtcagcagac cgaagaagag caggatcgcg ttgaagaccg tgaagacatt
      421 cgcgacgact atcgagcggt aggaccggct cgtctctgcc ttggggagcg gtccgcgctc
      481 ctccagccgg cgggctgcct cggcggcggt cagaccggtc gcgggggttc ccgttccggg
      541 attgccggcg ggatcctgtg ggtcggtcgg ggcctcggag acagtcaagc tgcccggact
      601 ctatttgccc gggcgaggtg gggtggacac cgatgggggt ggggagactt tcaagttccc
      661 gggactgaag tgggcatggg aacaagaaat cacccgcttg acatatttat gtctagatgg
      721 gattctgaaa gggatgagga ccaccgtgag gcttgatgag caactgatgc gggatgccaa
      781 ggcgcttgcc gcgaaggaag gcaagacctt cactgccctg atagaggact cgctccgcga
      841 gcgactcgcc agatcccgga atcgggaaac gcgggagcac aggagctttc cggtgtccaa
      901 gatgccggga ggattgcgtg agggactgga cccggaaatc ctgagcgaca actccaggct
      961 cgccgacgcc ctggacgagt tcgatggttt tgccggacgt taacgtcctg ctgagggcgt
     1021 ttcggcccga gcttcaagct cacgaggcct cggcagcctg gctccaggcc cagatcgacg
     1081 gtcccgcacc tttcgcgatg gccgaccggg tcatcgaagg gttcgttcgt atcgtgaccc
     1141 acccgggtgg gttcgatcac cctgaacctg caccaagcgc gcttgaattc gtcgagatgc
     1201 tcctcagccg ggacaactgc gtgagagtga acccggggcc gctccagtgg gagatcttcg
     1261 tgaatctggt tcgcgaaacg ggtgcggtcg ggaactcggt gtcagacgcg tggctggcag
     1321 ccctggcgat cgagtccggg tccgagtgga ttacctggga caaggggt
//
LOCUS       DAOTIP010000641         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_115196_length_1365_cov_3.901527, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000641 DAOTIP010000000
VERSION     DAOTIP010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115196_length_1365_cov_3.901527"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="P5138_10725"
     CDS             complement(<1..836)
                     /locus_tag="P5138_10725"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRV61095.1"
                     /translation="MDQLRWEMNPPKLRDPMMVCAFRGWNDAASAASSALETLADSLE
                     VDVLAEVDPEDFYDFQANRPTIRLEEGAHRRIEWPSNTFIAARAQGADRDLVLLDGTE
                     PNLKWRTYSEMVVQVAEQLDVQMVVVLGSLIAEVAHTLPVPITGVSTDEATVRSLELE
                     RSDYEGPTGIVGVIHDRCRAAGLPSVSLWAAVPHYVAAVPNPKAALALSEKLESITRV
                     AADISGLEDETMSYEEQIGRAVAADPEVEELVERIENEQRQRRGPELDVPSGDALAME
                     FQR"
     gene            924..>1365
                     /locus_tag="P5138_10730"
     CDS             924..>1365
                     /locus_tag="P5138_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRV61096.1"
                     /translation="MEAGTRNGTPVVELTPGQNFTGRYACARKDRLTAKNGSAYLALE
                     LRDRSGSIPARIFRDSDRIGIGFERGDVIAVKGRVEQFRGQLSAELEDVKKIDPAEID
                     PADFLPTAYRDREELEGFLEHLSREVHHPGYRAVVEDLLFTGETA"
BASE COUNT          217 a          463 c          452 g          233 t
ORIGIN      
        1 cgctggaact ccatcgcgag cgcgtccccg ctcggcacat ccagttcggg tccgcgacgc
       61 tgacgctgct cgttctcgat ccgttcgacc agctcctcga cctccggatc ggcggcgaca
      121 gcgcggccga tttgctcctc gtagctcatg gtttcgtctt cgaggccgga gatgtccgcg
      181 gcaacccggg tgatcgactc gagcttctcg cttagtgcga gggcagcctt tgggttcggc
      241 accgccgcca cgtaatgggg cacggcagcc cagagcgaga ccgacggcag tccggcagcc
      301 cggcagcggt cgtggatcac gccgacgatg ccggtcggcc cctcgtaatc agagcgttcg
      361 agctccaggc ttcgcaccgt cgcttcatca gtcgagacgc cggtgatcgg gaccggcagg
      421 gtgtgggcga cctcggcgat cagcgagccg agcacgacca ccatctgcac gtccagctgc
      481 tccgccacct ggaccaccat ctccgagtat gtgcgccact tgaggttcgg ttcggtgccg
      541 tcgagcagga ccaggtcccg gtcggcgccc tgggcccggg ccgcgatgaa ggtgttcgac
      601 ggccattcga tccggcgatg cgcaccttcc tcgagccgga tggtcgggcg attcgcctga
      661 aagtcgtaga aatcctcggg gtcgacctcg gccagaacgt cgacttcgag cgagtcggcc
      721 agggtctcca gggcggagga tgcggcgctg gccgcgtcgt tccagccgcg gaaggcgcag
      781 accatcatcg ggtcgcgcag cttcggcggg ttcatctccc agcggagttg gtccatgagt
      841 tcaatgtagc cgacgcgccc tctcggcccc gtggcatgcg gccacggttc gcgcgtctgc
      901 ggcttcggtc aggatgtgaa ccgatggaag ccggcaccag aaacggaacc cccgtggtcg
      961 aactgactcc cggccagaac ttcaccggcc gctacgcctg cgcgcgcaag gacaggctca
     1021 ccgcgaagaa cggatccgcc tacctcgctc tggagctgcg cgaccggtcc ggctcgatcc
     1081 cggcccgtat tttccgtgac tccgaccgga tcgggatcgg cttcgaacgg ggggacgtga
     1141 tcgccgtgaa gggccgggtc gagcagttcc gcggccagct ttcggccgag ctcgaggacg
     1201 tgaagaagat cgatccggcc gagatcgacc cggcggattt cctgcccacc gcctaccggg
     1261 accgcgaaga gcttgagggc ttcctcgaac acctttcccg cgaagttcac catccgggct
     1321 accgggcggt ggtcgaggac cttctcttca ccggtgagac agcgg
//
LOCUS       DAOTIP010000642         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_115481_length_1363_cov_2.706422, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000642 DAOTIP010000000
VERSION     DAOTIP010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115481_length_1363_cov_2.706422"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1363
                     /locus_tag="P5138_10735"
     CDS             <1..>1363
                     /locus_tag="P5138_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61097.1"
                     /translation="ALRNIHAQGPPAVDHDQDPDRGRRGAKIDGFEIHLAEIDGIKLI
                     DIRDFEAEYSAEEKKLGGSADFSLPFMGDKGVSFGFEIENLVVTKATVGVSGVKIPIG
                     APPAGFITALNGGFGFKQIDNYFVLNLNAGATAEFGPEVPTPWGKLVPLEVNSNLKIG
                     KEGQDFYFLFDGGVKVFRLPVGNVYLKIHTNSGVAFGFSIGIGFPSYSNNPNDPFYIG
                     SSVDGWIAKNRFQFEGKGKVRLIGLDIFDGRILINDKAAGACWKVTFFDGGAVYEYGS
                     KEVKTFGVSCGLDRYRENYPSATHRGVAAASADRPRKLTTGTREVILSARGKGDAPRF
                     KLTSTDGRSFKVPRGKDIVRTKKYMIVVDRKNKVTHVAAAGLANGNWTIAPYGNSVPI
                     MGVKTGKALPPERVQAQVVGKGLNRTLIWNSRGNPHTKLAFTEVMKGGYEQPILTTDK
                     VRGR"
BASE COUNT          280 a          430 c          431 g          222 t
ORIGIN      
        1 gctctccgga atattcacgc tcaagggcca cccgcagtcg accacgatca agatcccgac
       61 cgcggtcggc gaggcgcgaa gatcgacggc ttcgagattc acctggcaga gatcgacgga
      121 atcaagctga tcgacatcag ggacttcgag gccgaatact cggccgagga gaagaagctg
      181 ggcggcagtg ccgatttctc gttgccgttc atgggtgaca agggggtcag cttcggcttc
      241 gagatcgaga acctggtcgt gaccaaggcg acggtcggtg tctccggggt gaagatcccg
      301 atcggcgctc cgccggccgg tttcatcacc gccctgaacg gcggcttcgg tttcaagcag
      361 attgacaact acttcgtgct gaacctgaac gccggggcaa ccgccgaatt cggccccgaa
      421 gtgccgaccc cctggggcaa gctggtgccc ctcgaggtga actcgaatct gaagatcggc
      481 aaggagggcc aggacttcta cttcctcttc gacggcgggg tgaaggtgtt ccgcctgccg
      541 gtgggcaacg tctacctgaa gatccacacc aacagcgggg tcgcgttcgg cttcagcatc
      601 gggatcggat tccccagtta ctcgaacaac ccgaacgacc ccttctacat cggttcgtcg
      661 gtcgacggct ggatcgccaa gaacaggttc cagttcgagg gcaagggcaa ggtgcggctg
      721 atcggcctcg acattttcga cggccgcatc ctgatcaacg acaaggccgc tggcgcctgc
      781 tggaaggtca ccttcttcga tggcggtgcc gtctatgaat acggctccaa ggaggtcaag
      841 actttcgggg tcagttgcgg cctcgaccgc taccgggaga actacccctc ggccacccac
      901 cgcggagtcg ccgccgcttc ggccgaccgg ccccgcaagc tgaccaccgg aaccagggaa
      961 gtgatcctca gcgcgagggg caagggcgac gctccccgct tcaagctgac ctcgacggac
     1021 ggacgcagct tcaaggtccc gcgtggcaag gacatcgtcc gcacgaagaa gtacatgatc
     1081 gtggtcgacc ggaagaacaa ggtcacccac gtcgccgcgg ccggactcgc caacggcaac
     1141 tggaccatcg ccccctacgg gaactcggtc ccgatcatgg gcgtgaagac cggcaaggca
     1201 ctgccgccgg agagggttca ggcccaggtc gtcggcaagg gtctgaaccg gaccctgatc
     1261 tggaactcaa ggggcaaccc tcacacgaag ctggctttca ccgaggtgat gaagggcggc
     1321 tacgagcagc cgatcctgac caccgacaag gtccggggcc ggt
//
LOCUS       DAOTIP010000643         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_115483_length_1363_cov_2.691131, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000643 DAOTIP010000000
VERSION     DAOTIP010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115483_length_1363_cov_2.691131"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="P5138_10740"
     CDS             <1..770
                     /locus_tag="P5138_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRV61098.1"
                     /translation="DTGSHAESLPVLAQPRVKERRPSPWRKRFLVAAVLTLAIGLLMI
                     LAANLIVLLDSDGKIWNDPAEATHAEVAIVPGAMVNPDGTMSMMLADRVDQAAALYKD
                     GKVDKILVSGDHGQWTYDEPTTMRHALMAEGIPARVIYTDHAGFNTHATMQRAKDIFG
                     VTSAIIVTQGFHMKRSLFLAKAAGLSANGLTSDLHGYGGQGIKSDVREVASRAKAVFD
                     VLTGAPVMGGDPVPITGPAKASWGPKAPAGTPPAGAP"
     gene            complement(804..>1363)
                     /locus_tag="P5138_10745"
     CDS             complement(804..>1363)
                     /locus_tag="P5138_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV61099.1"
                     /translation="FRLSYAMEEARGISRGEQDFSVTTTAFKGEDGQVSTIEVAKADP
                     APPFAPVEGSNFEIKADLVLLAMGFLHPEQELLDGLGVDKDQRGNVKAGTYETSVPGV
                     FAAGDARRGQSLIVWAINEGRQAARMVDRYLGTLPSASPAPRKSDVHTGNVNPADEGP
                     EGPPKHVSPGLGPDPSASGPGFAQE"
BASE COUNT          235 a          446 c          458 g          224 t
ORIGIN      
        1 tggataccgg atcacatgcg gaatcacttc cagttctggc acagccgcgg gtgaaggagc
       61 ggcgcccctc gccgtggcgg aagcgcttcc tcgtcgctgc cgtgctgacc ctggcgatcg
      121 ggctgctgat gatcctggcc gccaacctga tcgtgctgct cgattccgac gggaagatct
      181 ggaatgaccc ggcggaggca acccatgccg aagtggcgat cgtgcccggc gcgatggtca
      241 accccgacgg gacgatgagc atgatgcttg ccgaccgggt cgatcaggcc gcggctctct
      301 acaaggacgg gaaggtcgac aagatcctgg tttccggcga tcacgggcag tggacctatg
      361 acgagccgac caccatgcgc cacgcactga tggccgaggg gattccggcc agggtgattt
      421 acaccgacca cgccggcttc aacacccacg cgaccatgca acgggcgaag gacatcttcg
      481 gtgtgaccag tgcgatcatc gtcacccagg gcttccacat gaagcggtcg ctgtttctgg
      541 ccaaggcggc aggtctcagc gccaacggcc tgacctccga tcttcacggc tacggcggcc
      601 agggaatcaa gagcgacgtc cgtgaagtcg cgtcaagggc gaaggcggtc tttgacgtac
      661 tgaccggcgc gccggtgatg ggcggcgatc cggtcccgat caccgggccg gccaaggcga
      721 gttgggggcc gaaggcgccc gcaggtactc cgcccgccgg ggcgccctag accggagccg
      781 acccgacggt cgcagctaac ttctcactcc tgggcgaagc ccggacccga ggcgcttggg
      841 tcggggccga gccccggaga gacatgcttc ggcggtcctt cagggccctc gtcggccggg
      901 ttcacgttcc cggtgtggac atccgacttg cgcggggccg gggaagcgga aggcagggtg
      961 cccagataac ggtcaaccat gcgggccgcc tgacggcctt cgttgatcgc ccagacgatc
     1021 agtgactggc cgcgtcgggc gtcgccggcg gcgaagacac cgggaaccga tgtctcgtag
     1081 gtccccgcct tgacgttacc gcgctggtcc ttgtcgaccc cgagcccgtc cagcagttcc
     1141 tgttcgggat gcaggaagcc catcgccagg aggaccagat cggccttgat ctcgaaattc
     1201 gagccttcaa ccggagcaaa cggcggcgcg ggatcggcct ttgcgacctc gatcgtgctt
     1261 acctgcccgt cctcgccctt gaaggcggtg gtcgtgacgg agaagtcctg ctcgcctcgg
     1321 gagattcccc tggcctcctc catcgcgtac gagaggcgga act
//
LOCUS       DAOTIP010000644         1356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_116344_length_1356_cov_2.939277, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000644 DAOTIP010000000
VERSION     DAOTIP010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116344_length_1356_cov_2.939277"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5138_10750"
     CDS             complement(<1..207)
                     /locus_tag="P5138_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRV61100.1"
                     /translation="MNRVGYIGLGIMGKPCTLNLITAGHELTVSTRTRSKADEAIAAG
                     ATWASTPSAVAGASEAVFLNLPDTP"
     gene            complement(204..1007)
                     /locus_tag="P5138_10755"
     CDS             complement(204..1007)
                     /locus_tag="P5138_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV61101.1"
                     /translation="MNEPATISPGAGPSRAPAALSIRSLTKRYSDGTLALEDLDLEIP
                     AGSFFGLLGPNGAGKTTLIGAVCNLIRVTGGEISVFGRPGTGMAARRMIGIAEQEPNL
                     DKFLTVYETLLYHGGYYGMDGEEASERAEQMIDAFDLRGKRDVEAPKLSGGMRRRLLL
                     ARALLHRPRLVILDEPTAGVDLELRHELWRYIRGLHEQGTTILLTTHYLEEAEALCDE
                     IALISDGHIVARDSAEGLKRSHGVDTLEEVYLRTVAGGRIAMDQLEEVE"
     gene            1024..>1356
                     /locus_tag="P5138_10760"
     CDS             1024..>1356
                     /locus_tag="P5138_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRV61102.1"
                     /translation="MSALSGVIVADFSRVLAGPLAAMTMGDLGANVIKVEPPDGDETR
                     SWLPPVDAEGRSTYFHTANRNKRSVKLDLKDEGDRALAFELAMRADVLIENFRPGTMA
                     RFGLDWETV"
BASE COUNT          216 a          442 c          456 g          242 t
ORIGIN      
        1 cggcgtgtcg ggcaggttga ggaagaccgc ttcgctggcc ccggccacgg ccgagggggt
       61 ggaggcccag gtcgcgcccg cagcgatcgc ctcgtcggcc ttcgaacggg tacgcgtgct
      121 gaccgtcagt tcgtgtccgg cggtgatcag gttcagcgtg cagggtttgc ccatgatgcc
      181 gaggccgatg tagcccactc ggttcattcg acttcctcca actggtccat agcgatcctt
      241 cctcctgcca ccgtccgcag gtacacctct tcaagggtgt ccaccccatg ggagcgcttc
      301 agtccctccg ccgaatcccg ggcgacgatg tgcccgtcgc tgatcagtgc gatctcgtcg
      361 cagagagctt cggcttcctc cagatagtgg gtggtcagaa ggatcgtcgt gccctgctcg
      421 tggaggcccc gaatgtagcg ccagagctcg tgccgtaact caaggtccac gcccgcggtc
      481 ggttcgtcga ggatgaccag gcgcggccgg tgaagcaggg cccgggcaag gagcagccgg
      541 cgtcgcatgc ccccggagag cttcggagcc tcgacgtccc gtttgccccg gaggtcaaag
      601 gcatcgatca tctgttcggc ccgctccgat gcctcctcgc cgtccatgcc gtagtagccg
      661 ccgtggtaga gcagcgtctc gtaaacggtg aggaacttgt cgaggttcgg ctcctgttcg
      721 gcgatcccga tcatccgacg ggccgccatc ccggtgccgg gacggccaaa gacggagatc
      781 tctccgccgg tcacccggat caggttgcag accgccccga tcagggtggt cttgccggcc
      841 ccgttcggac caagcaggcc gaagaagctg ccggcgggga tttccaggtc gaggtcttcc
      901 agggccaggg tcccgtccga gtagcgtttg gtcagtgacc ggatcgaaag ggcggccggc
      961 gcccgggagg gtccggcccc cgggctgatt gtcgcgggct cgttcatgcg tcaccatgca
     1021 accatgagcg cactctccgg cgtaatcgtg gcggacttca gccgggtcct ggccgggccg
     1081 ctggcggcaa tgaccatggg tgatctcggc gccaacgtga tcaaggtcga gccaccggac
     1141 ggcgatgaaa cccgctcctg gctgcctccg gtcgacgccg agggccgttc cacctacttc
     1201 cacaccgcga accgcaacaa gcggtcggtg aaactcgatc tcaaggatga aggagatcgg
     1261 gcactggcct tcgagttggc gatgagggcg gacgtgctga tcgagaactt ccggcccggg
     1321 accatggccc gtttcgggct cgactgggag accgtt
//
LOCUS       DAOTIP010000645         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_116855_length_1352_cov_2.692367, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000645 DAOTIP010000000
VERSION     DAOTIP010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116855_length_1352_cov_2.692367"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="P5138_10765"
     CDS             <1..522
                     /locus_tag="P5138_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV61103.1"
                     /translation="ESATDLGPMVSAIQRGKAVGQVEDAVASGAELLTGGGNAGFEKG
                     HYLEPCVLTGVKPEADLLREETFGPVAPIVPVKDLDEAIALANGLDFGLGANIYTRDL
                     KRAIRCVKEVRSGSVWINDPLTDNDAGPFGGFKNSGIGRELGTEGLDAFREAKHAHID
                     TEIEVKEWWYPYG"
     gene            complement(749..1237)
                     /locus_tag="P5138_10770"
     CDS             complement(749..1237)
                     /locus_tag="P5138_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61104.1"
                     /translation="MAGSDGGGGSGGEDVSKEEYIAKADAICAQYAPDSVTLERQFNQ
                     SLKSSDLESAAQDFADQAADFSAMLDELEGLNAPEADQATVTQIIALGRQRVDAAKQA
                     ADAIASGDKDTMIAAGKKASVLAGKYYQLADGFGFQACGSTGSTGTTGSTGPTGVTGT
                     TS"
BASE COUNT          219 a          455 c          441 g          237 t
ORIGIN      
        1 gagtcggcca cggacctggg accgatggtc tcggcgatcc agcggggcaa ggccgtcggg
       61 caggtcgaag acgcggtcgc ttcaggtgcc gagttgctga ccgggggcgg aaacgccggg
      121 ttcgagaagg gccattacct cgagccctgc gtgctcaccg gcgtgaaacc ggaagcggac
      181 ctgctccgcg aggagacctt cggcccggtt gccccgatcg ttccggtcaa ggacctcgac
      241 gaggcgatcg cactggccaa cggcctcgac ttcgggctcg gagccaacat ttatacccgc
      301 gacctgaagc gggccatccg ctgcgtcaag gaggtgcggt ccggctcggt ctggatcaac
      361 gatccgctga ccgacaacga cgccggtcct ttcggcggct tcaagaattc aggaatcggc
      421 agggaactcg gcaccgaagg cctggatgcc ttccgcgagg ccaagcatgc ccacatcgac
      481 accgagatcg aagtcaagga gtggtggtac ccctacggct gatgcccgga ggcggaggcc
      541 ccgggagcgt tcgtcctatc ccgtcgacta gttgctgtcg ccggcgctgc cgcaaccggt
      601 gaagatgaca actccggtca tggcggctac accgagcacc gcaaggcgcc cggaagtgga
      661 cttgacacgc atttgatctc cctttcgggt ttggggaact cgagcgtata ccggacctgc
      721 cgccaggtgg ccccgatcgg accccgggtc aggaggtggt gccggtgacc ccggtggggc
      781 ccgtggatcc ggtcgtgccg gtgctgccgg tgctgccgca ggcctggaag ccgaagccgt
      841 cggccagttg gtagtacttg ccggccagca cggatgcctt cttgccggcc gcgatcatcg
      901 tgtccttgtc ccccgaggca atcgcgtcgg cggcctgctt ggccgcatcc acccgctgcc
      961 tgccaagcgc gatgatctgg gtcacggttg cctgatcggc ctcgggcgcg ttcaacccct
     1021 ccagctcgtc gagcatggcc gagaagtcgg cggcctgatc cgcgaaatcc tgtgccgcac
     1081 tctcgaggtc actgctcttg agtgactggt tgaactgccg ctcgagcgtg accgaatccg
     1141 gcgcgtactg cgcacagatc gcgtcggcct tggcgatgta ttcctccttc gacacgtctt
     1201 cgccgcccga gccgcctccg ccatccgaac cggccatcgc ttctgccggg tcgtcagacg
     1261 cctcttcccc tcctgctggg ccggtgaacc tcctgcgcca gatcaaacag caaatccacg
     1321 acgaactgcg actcgtcgcc tcggtcgggg tg
//
LOCUS       DAOTIP010000646         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_116980_length_1351_cov_2.707562, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000646 DAOTIP010000000
VERSION     DAOTIP010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116980_length_1351_cov_2.707562"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="P5138_10775"
     CDS             <1..404
                     /locus_tag="P5138_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61105.1"
                     /translation="VMVPYWHETLESGEGDEIGLEFARLWDRLPKYVASRTLTEVHES
                     CRLIEGDAIEAILDLKRETEGDIEVGGGILSAQLDALGEIDVYNVWTLPVRIGGGKHY
                     FPAGRSVETFELTDLRRFPEGGVALEYTLRR"
     gene            complement(394..1344)
                     /locus_tag="P5138_10780"
     CDS             complement(394..1344)
                     /locus_tag="P5138_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRV61106.1"
                     /translation="MPVPEPGPGQVLMRVSTCGVCRTDLHIVDGELTKPGLPLVPGHQ
                     IVGTVEEFGPGTGAGDSGRRFAKGDRIGVPWLGWTDGHCHFCRQGRENLCENARFTGY
                     DIDGGYAEYAVADERFCFPLPGEIDDEHVAPLLCAGMIGYRALTIAGDGERLGLYGFG
                     SSAHILCQIAASEGRRVFAFTRPGDLDGQAFARSLGAEWAGGSDEQPPEPLDAAIVFA
                     PVGSLMVDALKVSDRGARIVSAGIHMSDIPSFPYELLWEERSLHSVANLTRQDGTELL
                     ELAARHQVEPMVETMPLEDANTALDAIRSGASEGSTVLSV"
BASE COUNT          243 a          433 c          455 g          220 t
ORIGIN      
        1 aggtgatggt cccctactgg cacgaaacgc tggagtcagg ggagggcgac gagatcgggc
       61 tcgagttcgc ccggctctgg gaccgactgc ccaagtatgt ggcctcccgg accctgaccg
      121 aagtccacga gagctgccgg ctgatcgagg gtgacgcgat cgaggcgatc ctcgatctga
      181 aacgggaaac cgagggggac atcgaggtgg gcggcgggat cctctccgcc caactcgacg
      241 cgctcggcga gatcgacgtc tacaacgtct ggacgcttcc ggtcaggatc ggcggcggga
      301 agcactattt cccggcaggc cggtcggtcg agacattcga gctgaccgac cttcggcgtt
      361 tccccgaggg cggggtcgcc ctcgaataca cgctcagacg ctgagtacgg tcgagccttc
      421 ggaggctccg ctgcggatcg cgtcaagggc ggtgttcgcg tcttcgaggg gcatcgtctc
      481 gaccatcggc tcgacctgat ggcgggcggc cagttcgagc agttcagttc cgtcctgccg
      541 ggtcaggttc gccaccgaat gaaggctgcg ctcttcccac agcagctcgt aggggaagga
      601 aggaatgtcg ctcatatgga tgccggcgct gacgatcctg gctccgcggt cactcacctt
      661 cagggcatcg accatcaggg aacccaccgg ggcgaagacg atcgctgcgt caaggggttc
      721 gggcggttgc tcgtccgaac cgccggccca ttccgcaccg agggaacggg caaaggcctg
      781 gccgtccagg tcccccggcc tggtgaatgc gaagacccgc cgcccttcgg aggcagcaat
      841 ctggcaaagg atgtgggccg aggagccgaa tccgtagagc ccgagccgtt caccgtctcc
      901 ggcgatcgtc agcgcccggt agccgatcat gccggcgcag agcaggggtg ccacatgttc
      961 gtcgtcgatc tccccgggaa ggggaaagca gaagcgctcg tcggcaaccg cgtattccgc
     1021 atatccacca tcgatgtcat agccggtgaa gcgggcgttc tcgcagaggt tctccctgcc
     1081 ctggcggcag aaatgacaat gcccgtcggt ccagcccagc cacggaaccc cgatccggtc
     1141 gcctttggca aaccgccgcc ctgaatcacc cgccccggtg ccagggccga actcttcgac
     1201 ggttccgacg atctgatggc cgggtacaag cggaagtccg ggtttggtca gttcgccatc
     1261 gacgatgtga agatcggtgc ggcagacgcc gcaggtcgag acgcgcatca ggacctggcc
     1321 cggacccggt tcgggcaccg gcaggtcccg a
//
LOCUS       DAOTIP010000647         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_117745_length_1345_cov_3.231008, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000647 DAOTIP010000000
VERSION     DAOTIP010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117745_length_1345_cov_3.231008"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5138_10785"
     CDS             <1..270
                     /locus_tag="P5138_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV61107.1"
                     /translation="AEEITMVEVVEALEGQIAPMECFHETPEGKVLCSHEADSDRACA
                     TKLLWTRVQGGVTKALAGTTLAELVEFSRPAEERSKSKTAVGSAA"
     gene            304..1068
                     /gene="sufC"
                     /locus_tag="P5138_10790"
     CDS             304..1068
                     /gene="sufC"
                     /locus_tag="P5138_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRV61108.1"
                     /translation="MAELEIKNLHVSVEEKEILNGLDLTVEKGRVHALMGPNGSGKST
                     LANVIMGHPALEVTEGSIVFDGEDITDADPDERAQLGLFMAFQYPVSIPGVAVSKYLR
                     MILNAQREARGEQPLKIKEFAAQAREAMDLANIPQDFSSRYLNDGFSGGEKKRMELLQ
                     LALLKPQIAILDETDSGLDIDALRTVAEGINKFAGPDMGALVITHYQRLLHMVEPDVV
                     HVMYEGRIVKEGGKELVDQLEAEGYGWIKEEVEAAA"
     gene            1133..>1345
                     /locus_tag="P5138_10795"
     CDS             1133..>1345
                     /locus_tag="P5138_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV61109.1"
                     /translation="MSVDEIRAQFPILEREVKGRPLVYLDNGATAQKPTAVIEAMNAY
                     YREHNANVHRGVHTLSEEATDLYEGAR"
BASE COUNT          268 a          453 c          430 g          194 t
ORIGIN      
        1 gcggaggaaa tcaccatggt cgaggtggtc gaggccctcg agggccagat cgccccgatg
       61 gagtgcttcc acgagacccc ggaaggcaag gtcctctgct cgcatgaagc agacagcgac
      121 cgggcctgcg cgacgaagct gctctggacc cgggtccagg gtggcgtgac caaggccctg
      181 gccggcacca cgctggccga gctggtcgag ttctcgcgcc cggccgaaga gcgcagcaag
      241 agtaagaccg cggtcggcag cgcagcctag ccgccgcccc agacttttcg aacggagcaa
      301 ccagtggcag aacttgaaat caagaacctt cacgtcagcg tcgaggaaaa ggaaatcctc
      361 aatggcctcg acctgaccgt cgaaaagggc cgggtccacg cgttgatggg cccgaacggt
      421 tccggcaagt cgaccctcgc caacgtgatc atgggccacc ccgccctcga ggtcaccgag
      481 ggttcgatcg tcttcgacgg cgaggacatc accgacgccg acccggacga gcgtgcccag
      541 ctcggcctct tcatggcctt ccagtacccg gtttcgattc ccggcgtggc ggtcagcaag
      601 tacctgcgga tgatcctcaa cgcccagcgt gaggcccgcg gcgagcagcc gctcaagatc
      661 aaggagtttg ccgcccaggc ccgggaagcg atggatctcg ccaacatccc ccaggatttc
      721 tccagccgtt acctcaatga cggcttctcc ggcggcgaga agaagcgcat ggagctgctc
      781 cagctggcgc tgctgaagcc ccagatcgcg atcctcgacg agaccgactc cgggctcgac
      841 attgacgcgc tccgtaccgt cgccgagggc atcaacaagt tcgccggccc cgacatgggc
      901 gccctggtca tcacccacta ccagcgcctg ctccacatgg tcgagccgga tgtggttcac
      961 gtgatgtacg aaggccgcat cgtcaaggaa ggcggcaagg aactcgttga ccagctcgaa
     1021 gccgagggct acggctggat caaggaggag gtcgaggcgg ccgcctgatg gtggccggcc
     1081 ccgcctccga gaccgcgccg gccgggtcca tgccggtgaa cggaccgggt caatgtcggt
     1141 agacgaaatc cgcgcgcagt tcccgatcct tgagcgcgag gtcaagggca ggccgctggt
     1201 ctacctggac aacggcgcga ccgcccagaa gccgaccgcg gtcatcgagg cgatgaacgc
     1261 ttactaccgg gagcacaacg cgaatgtcca tcgcggcgtc cacaccctct ccgaggaagc
     1321 caccgacctc tacgagggcg cgcgc
//
LOCUS       DAOTIP010000648         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_117806_length_1345_cov_2.060465, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000648 DAOTIP010000000
VERSION     DAOTIP010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117806_length_1345_cov_2.060465"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1345
                     /locus_tag="P5138_10800"
     CDS             25..>1345
                     /locus_tag="P5138_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61110.1"
                     /translation="MRNNSDRSRRAELIRDAHHEVLSALGYVWRREGAYTALNGAPVI
                     PLVSRAADASGREALWVIEAPIPAPEDEASDPLGACFTEDQFPPDQREAAMTDRTIES
                     VLAEGVFELSDGPRHVLVLGLSQIALIDKRKWPARSVLRFDLQEIFARADRDTLTVMA
                     CLISREARVPDQGAPISDRLEEEAQRNANAVTTSLKRTVRDAIELLGQEVIAVTGGKY
                     PSTFPDPGRRGVWIDGPELSRECLRYMYRLLFLFYAEANPRLNLLDMRNPIYATGYSL
                     EALRELESVPLRTTADREGTFLWESLQRTLELLYTGLDLADEERGTGLRLPSVKVSLL
                     DPESTPLLKGLGLRNEAVQQVIRLLSLRSTGKSTGRISYAKLGIGQLGAVYETLISFT
                     GVVAKTDLIELKPRKGRSNDAAEDGEEAEAPEPEEDAEEDLFGEDEAE"
BASE COUNT          234 a          431 c          457 g          223 t
ORIGIN      
        1 ccgaccgtcg aagcgctgcg ccaggtgcgc aacaattcgg atcggtcccg gcgcgccgaa
       61 ctgatccggg acgctcacca cgaagtgctg tccgccctcg gctacgtgtg gcggcgcgaa
      121 ggggcctaca cggctctgaa tggagcgccg gtaatcccac tcgtgtcccg cgccgcagat
      181 gcaagcggtc gcgaagcgct ctgggtgatc gaggcgccga tcccggctcc ggaggatgag
      241 gcatcggacc cactgggcgc ctgcttcact gaagatcaat tcccgccgga ccagcgcgag
      301 gcggctatga ccgaccggac catcgagtcc gtcctcgccg agggggtgtt cgagctgtcc
      361 gacggccctc gtcacgttct tgtcctcggc ctctcgcaga tcgcgctgat cgacaagagg
      421 aaatggccgg ctcggtccgt cctccggttc gacctccagg agatcttcgc ccgggcggac
      481 agggacacgc tcaccgtgat ggcctgcctg atctcccgcg aggcgcgcgt cccggatcag
      541 ggggcaccga tttctgatcg gctcgaagag gaggcgcagc ggaacgccaa cgcggtcacg
      601 acatctttga aacgaacagt ccgggacgcg attgagctgc tcggccagga ggtgatcgcg
      661 gtcactggcg ggaagtaccc atccaccttc ccggacccgg gccggcgcgg ggtctggatc
      721 gacgggccgg agctgtcgcg cgaatgcctg cgctatatgt accggctcct gttcctgttc
      781 tacgcggagg cgaacccccg tctgaacctg ctggacatgc ggaacccgat ctacgcgact
      841 ggatattcgc tcgaggcgct gcgtgaactg gaatccgttc cgctccggac cacggcggac
      901 cgggagggga cgttcctctg ggaaagcctt cagcggacgc ttgagctttt gtatacaggc
      961 cttgacctcg ccgatgagga gcggggcacc ggccttcgcc tgccgtccgt gaaggtgtcc
     1021 ctgctcgacc cggagagcac gccgctcttg aaagggctgg ggctgcgcaa cgaggcggtt
     1081 cagcaggtga tccggctgct gtcgctccgg tccaccggca agagcactgg ccggatttcc
     1141 tacgcgaagc ttgggatcgg ccagctgggc gcggtctatg agaccctgat ttcgttcacc
     1201 ggagtggtcg ccaagaccga cctcatcgag cttaaacccc gcaagggccg gtcgaatgac
     1261 gcggctgagg acggggagga ggcggaagcg cccgaaccgg aagaggacgc cgaggaggac
     1321 cttttcggcg aagatgaggc cgaac
//
LOCUS       DAOTIP010000649         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_118037_length_1343_cov_3.144410, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000649 DAOTIP010000000
VERSION     DAOTIP010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118037_length_1343_cov_3.144410"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="P5138_10805"
     CDS             <1..602
                     /locus_tag="P5138_10805"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRV61111.1"
                     /translation="NLELPASEEIAPEPLCQYGMSKLAGEQYLALYRRMYMFNSVALR
                     FGNVYGPRQSPKGEAGAVAIFGELLMAGQNPVVFGDGLQTRDFVYIDDLIEAILTASR
                     TDVEGPINLGSGEETSILDLLSGLREAGLSLNGDGPPEGTDFEPSFDRGRPGEVRRIS
                     IDPARAARELGWNPTTQLKSGLRETLSAMAASADDITHA"
     gene            655..>1343
                     /locus_tag="P5138_10810"
     CDS             655..>1343
                     /locus_tag="P5138_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRV61112.1"
                     /translation="MPAQAYAGKECVCQYRKPVCDQTCVRDMLETPFYIACLKLTGRR
                     CVVVGGGEVGLEKVEGLLACDADVTLIAPFAVQPLEQMAAEGSVRWERREYAGRQDLE
                     GVFMVIAATDDTDVNIRIFDEAEERAMLVNVVDVPPLCNFILPAIVRSGPLAIAISTA
                     GASPALAKRMKNEIAGLYGEDHARLAVILNEARGWAKATLPTYQDRKVFFEGIVNGDP
                     DPIVLIGGDREP"
BASE COUNT          236 a          474 c          429 g          204 t
ORIGIN      
        1 gcaacctcga actgccggcc tccgaggaaa tcgccccgga gccactctgc cagtacggca
       61 tgagcaagct cgcgggagag cagtacctgg ccctgtaccg gcgcatgtac atgttcaact
      121 cggtggcgct ccgcttcggc aacgtctacg gcccgcgcca gagcccgaaa ggagaggccg
      181 gcgcggtcgc gatcttcggc gagctgctga tggccggaca gaacccggtc gtcttcggcg
      241 acggcctgca gacccgcgac ttcgtctaca tcgacgactt gatcgaagcg atcctcaccg
      301 cctcccgcac cgacgtcgag ggcccgatca acctcggatc cggcgaggag acctcgatcc
      361 tcgacctgct cagcggtctc cgcgaagccg gcctctcact taacggtgac ggcccgcccg
      421 agggaacgga ctttgaaccg tcattcgacc gtggccgacc gggagaggta cggcggatct
      481 cgatcgaccc cgcccgcgcc gcccgcgagt tgggctggaa ccccaccacc cagctcaagt
      541 ccggcctccg ggaaaccctc agtgcgatgg ccgccagcgc cgacgacatc acccacgcct
      601 gatccactcc gggcttccgg aagcgctttt cgcaggtccc ctaacctttg aggcatgccc
      661 gcccaggcct atgccggaaa ggaatgcgtc tgccagtacc gcaaaccggt ctgcgaccag
      721 acctgcgtgc gggacatgct cgagacccct ttctacatcg cctgcctgaa gctgaccgga
      781 cgcaggtgcg tggtggtcgg cggcggcgag gtcgggctcg agaaggtcga aggcctgctc
      841 gcctgtgacg ccgacgtcac cctgatcgcg cccttcgcgg tccagccgct ggagcagatg
      901 gcggccgagg gatcggtccg ctgggagcga cgcgagtacg ccggaaggca ggacctcgaa
      961 ggcgtcttca tggtgatcgc cgcaaccgat gacaccgacg tcaacatccg catcttcgac
     1021 gaggcggagg aacgggcgat gctggtcaac gtggttgacg tgccgccgct ctgcaacttc
     1081 atcctgccgg cgatcgtccg ctccggcccg ctggcgatcg cgatctccac cgcgggcgcc
     1141 tccccggccc tggccaagcg gatgaagaac gagattgcgg gcctctatgg cgaggaccac
     1201 gcacgcctgg cggtgatcct gaacgaggcc cggggctggg cgaaggcgac cctccccacc
     1261 taccaggacc gcaaggtttt cttcgagggg atcgtgaacg gcgaccccga tccgatcgtc
     1321 ctgatcggcg gggaccgcga acc
//
LOCUS       DAOTIP010000650         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_118149_length_1342_cov_4.385392, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000650 DAOTIP010000000
VERSION     DAOTIP010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118149_length_1342_cov_4.385392"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="P5138_10815"
     CDS             complement(<1..230)
                     /locus_tag="P5138_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRV61113.1"
                     /translation="MSSRDEIIAFCDELLEIDGFGDYGPNGLQVPGSLEVGRIATAVS
                     AHKESITAAVQAGAEMLLTHHGLFWDFHSRALT"
     gene            297..560
                     /locus_tag="P5138_10820"
     CDS             297..560
                     /locus_tag="P5138_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61114.1"
                     /translation="MAEEPETEVAAPSDAFIESLMDTDLYSMGAYFSDRNPELIDELI
                     DRSVAIEERGLVRFSDEEDVELEEAFKTLLTGLAVRYYKSVTG"
     gene            562..1278
                     /locus_tag="P5138_10825"
     CDS             562..1278
                     /locus_tag="P5138_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRV61115.1"
                     /translation="MSRLRQRRPGQYRTLIILAVAIGALLIALIGMATRKASNEYEDI
                     QGTQDMREIFGGVRQLDDRLGSDDAPVQMQVFLDAQSGTYRDQFLDTVPALVNGPVRD
                     GNLKLLYRNRSLTRNATERSFYGIQAAQNQGYGWQYAYLMFRNQDLAREQGLDDDFLT
                     KLAESIEYLDQDEWKKDFEQGLEEGSAMNADLQAQDQIAIDLKIRDEPAVVVNGPGGT
                     EIVQDAPDLAEIQSAISAVQ"
BASE COUNT          262 a          420 c          435 g          225 t
ORIGIN      
        1 gtcagggccc gcgaatggaa gtcccagaac aggccgtgat gggtcaggag catttcggct
       61 ccggcctgga cggctgccgt gatcgactcc ttgtgggccg agacggcggt cgctatccgt
      121 ccgacctcga gactgccggg cacctggagc ccgttcggtc cgtagtcgcc gaagccgtct
      181 atctcgagca actcgtcgca gaaagcgatg atttcgtccc gggaggacac cgcttcacca
      241 tataagtgtg gcggccctgc cccttcccgg ggcccgtctg tccctaacat gtgctcgtgg
      301 ctgaagagcc ggaaacagaa gtggctgcgc ccagcgacgc cttcatcgaa tccctgatgg
      361 acaccgatct ctattcgatg ggtgcctatt tctccgatcg caacccggaa ctgatcgacg
      421 agctgatcga tcgcagcgtg gcgatcgagg agaggggcct ggtccgcttc tccgacgaag
      481 aagacgtcga gctggaggaa gcgttcaaga cgctgctcac cggtctcgcg gtgcggtact
      541 acaagtcggt cactggttga gatgagtcgt ctcaggcagc gccgtcccgg tcaataccgg
      601 acgctgatca tcctcgccgt cgcaatcggc gccctcctga tcgccctgat cgggatggcg
      661 acccggaagg cgagcaacga gtacgaggac atccagggaa cccaggacat gcgggagatc
      721 ttcggcgggg tacggcaact cgacgaccgg ctcggctccg atgacgcgcc ggtgcagatg
      781 caggtcttcc tcgacgcgca gagcggcacc taccgggacc agttcctcga caccgtcccg
      841 gccctggtca acgggccggt gcgcgacggg aacctgaaac tgctctacag gaaccggtcg
      901 ctgacccgga acgcgacgga acggagcttt tacggaattc aggccgccca gaaccagggc
      961 tacggctggc agtacgccta cctgatgttc cgcaaccagg acctggccag ggagcagggg
     1021 ctggacgacg attttctgac caaactggcc gagtcgatcg aatacctcga ccaggacgag
     1081 tggaagaagg acttcgagca gggcctcgag gaaggcagcg ccatgaacgc cgatctccag
     1141 gcccaggacc agattgcgat cgatctgaag atccgggacg aaccggccgt ggtcgtcaac
     1201 ggccccgggg gcacggaaat cgtccaggac gcacccgacc tggccgagat tcagtcggca
     1261 atctcggcgg tccagtaggg ccggtcagga cgggttcccg accgggtttt ctgtaggctc
     1321 acttctcctc ggggcgtggc gc
//
LOCUS       DAOTIP010000651         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_118361_length_1341_cov_2.150855, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000651 DAOTIP010000000
VERSION     DAOTIP010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118361_length_1341_cov_2.150855"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="P5138_10830"
     CDS             <1..618
                     /locus_tag="P5138_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61116.1"
                     /translation="FGARNLAPVWAGLPLLIAAGLAAAGPRWGPVALVLVLAGFSVTT
                     ARLLDPAKTSISYEKAAESIESDQDRGGAVLDAAIVSPAPLTPLDGYLDTDLREFRMT
                     NIEDRPDFIKDIFRGYDPQQIADQAFDTAGPVRVVTLGEGSAPVRDGQGGYSFLASGT
                     EVLVPAGWREQKRVSFEGVEQLSVSTFVRSGRGSGGGGQKTGDAQ"
     gene            615..1319
                     /locus_tag="P5138_10835"
     CDS             615..1319
                     /locus_tag="P5138_10835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV61117.1"
                     /translation="MTEPQPHQHVHFNEIADEYDDSLPPHVVEHYLAKRVAYVRRILP
                     SGKVLDVGCGTGVVAGRLAEAGYEVTGVDPSAGMLRYVEEHAPGVTAIEGSATDLPFE
                     DGTFDLTMCVAVLHHIIEPEAVHQSLVEMVRVTKPGGLVLVWDHNPRNPYWKNLMARV
                     PQDDGTERLIPEAEILDGLADGGAKVIRSDQLGLVPDFVPPRLLGLAAGSEKVAEKVP
                     GVRRLCAHNVVLARRP"
BASE COUNT          208 a          458 c          460 g          215 t
ORIGIN      
        1 ttcggtgcgc gcaacctcgc tccggtctgg gcgggcctgc cgctcctgat cgccgccggc
       61 ctggccgcgg ccggcccccg ctggggaccg gtggcactgg tgctggtcct ggccggtttc
      121 tcggtcacca cggcccggct cctcgatccg gcgaaaacat cgatctctta cgagaaggca
      181 gccgagtcga tcgagtcgga tcaggatcgg ggcggcgcgg tgctcgacgc ggcgatcgtc
      241 agtccggcgc cgctcactcc cctcgacggg tacctcgaca ccgacctgcg cgagttccgg
      301 atgaccaaca tcgaggaccg tcccgatttc atcaaggaca tattccgggg gtacgatccg
      361 cagcagattg ctgatcaggc attcgacact gcgggcccgg tccgggtcgt caccctcggc
      421 gagggttccg ccccggtgcg ggacggtcag ggcggctact ccttcctggc cagtggtacc
      481 gaggtgctgg taccggccgg ctggcgcgag cagaagcggg tcagcttcga gggcgtggaa
      541 caactgagcg tgagcacctt cgttcggagc ggaagaggtt ccggaggggg cgggcagaag
      601 accggtgatg cccagtgacc gaaccgcagc ctcaccagca cgtccacttc aacgagatcg
      661 ccgacgaata cgacgactcg ctgcctcccc atgtggtcga gcactacctg gcgaaacggg
      721 tcgcctatgt caggcggatc ctgccgtccg gcaaggtcct tgacgtcggc tgcggcaccg
      781 gggtcgtggc cgggcgcctg gccgaggcgg gctacgaggt gaccggagta gatccgtcgg
      841 ccgggatgct ccgttacgtc gaggagcatg cccccggcgt gacggcgatc gagggctcgg
      901 ccaccgacct gccctttgag gacggtacct tcgacctgac catgtgcgtg gcggttctcc
      961 accacatcat cgaaccggaa gcagtccacc agtccctggt cgagatggtc cgggtgacga
     1021 agcccggcgg gctggtcctg gtctgggacc acaacccccg caacccctat tggaagaacc
     1081 tgatggcccg ggtcccccag gatgacggca ccgaacgcct gatccccgag gccgagatcc
     1141 tcgacggtct cgccgacggc ggtgcgaagg tgatccgcag cgaccagctg gggctggtgc
     1201 ccgacttcgt gccgccccgt ttgctcgggc tcgcggccgg cagcgagaag gtcgccgaga
     1261 aggtccccgg cgtccggcgc ctctgtgccc ataacgtggt gctggcccga cggccatgac
     1321 ccgccgcacc gccggtctcc t
//
LOCUS       DAOTIP010000652         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_119466_length_1332_cov_3.657009, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000652 DAOTIP010000000
VERSION     DAOTIP010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119466_length_1332_cov_3.657009"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1101)
                     /gene="mraY"
                     /locus_tag="P5138_10840"
     CDS             complement(127..1101)
                     /gene="mraY"
                     /locus_tag="P5138_10840"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRV61118.1"
                     /translation="MGEIVIGAMASMLICLFLAPRFIEFLRGREFGQHIREEGPAGHQ
                     TKAGTPTMGGLIIFTAIIVPFLVLSERDAASMTVFGVALGCAALGFADDYIKIIKRRS
                     LGLPGRYKLLGQVFLALILWWVARHEVGLEPVIHFRIADLTWDIGPVAYFILVFLVLA
                     GATNGVNLTDGLDGLAAGSSAIVLLTYTAISYVTVGQHDLALFAVCLVGGCIGFLWFN
                     AFPATIFMGDTGSLGLGGAIGALAVMTQTEVLLIVIGGIFVIEALSVLVQVFSFKTFG
                     RRVLMMAPLHHHFEMMAWSETKIMLRFWIIAAVCSGIGFAIYQHSIGR"
     gene            complement(1094..>1332)
                     /locus_tag="P5138_10845"
     CDS             complement(1094..>1332)
                     /locus_tag="P5138_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61119.1"
                     /translation="FHEEIGAFARELEVEVIGVGDLARSYDPDLKAGSAEEAARMLEE
                     RLGPDLAVLVKGSRAAGLERVTELVTNGSGVGDG"
BASE COUNT          275 a          436 c          413 g          208 t
ORIGIN      
        1 tctgactccc ttcgagccgc gtcaaccccg ttcggtgaaa cgcattcagt gccatcaagg
       61 gaacaaatga gttacaccca ccgtgagcgg gtcgcaccca ggagggcccc ggctgcgttg
      121 cccgggctag cggccgattg aatgctggta gatggcgaag ccgatcccgg agcagaccgc
      181 ggcgatgatc cagaagcgga gcatgatctt ggtctcggac caggccatca tttcgaagtg
      241 gtgatggagc ggagccatca tcaggacccg gcgcccgaag gtcttgaacg agaagacctg
      301 gaccaggacc gagagcgcct cgatcacgaa gattccgccg atcacgatca gcagcacctc
      361 ggtctgggtc atcaccgcga gcgcaccaat ggcccctccc aacccgagcg acccggtgtc
      421 ccccatgaag atcgttgccg ggaaggcgtt gaaccagagg aagccgatgc agccaccgac
      481 cagacagacc gcgaacagcg cgaggtcatg ctggccgacg gtcacgtagg agatcgccgt
      541 gtaggtcagc aggacgatcg cacttgaacc ggcggccagt ccgtcgagac cgtcggtcag
      601 attgacgccg ttggtcgcgc cggccagtac gaggaagaca aggatgaaat aggcgaccgg
      661 ccctatgtcc caggtcaggt cggcgatccg gaagtggatg accggttcga gaccgacttc
      721 atgccgggcg acccaccaga ggatcagggc gaggaagacc tggccgagca gcttgtatcg
      781 gccgggcaat cccagtgaac gccgcttgat gatcttgatg tagtcgtcgg cgaacccgag
      841 cgcggcgcag ccgagcgcca ccccgaagac ggtcatcgat gccgcgtccc gctcggaaag
      901 caccagaaac ggaacgatga tcgcggtgaa gatgatcagc ccgcccatgg tcggggtgcc
      961 ggccttggtc tgatgaccgg ccggtccttc ctcccggatg tgctgtccga actcgcgacc
     1021 gcgaaggaac tcgatgaacc ggggtgccag aaagaggcag atcagcatcg aggccatcgc
     1081 cccgataacg atttcaccca tccccgaccc cgcttccgtt ggtgaccaac tcggtcaccc
     1141 tttccaggcc ggccgcccgg gatcccttga ccagtaccgc caggtccggg ccgagccttt
     1201 cctcgagcat tcgggccgct tcctccgcgc ttcccgcctt caggtccgga tcgtaggagc
     1261 gggccaggtc cccgactccg atcacttcga cctccagctc acgggcaaag gccccgattt
     1321 cctcgtggaa ac
//
LOCUS       DAOTIP010000653         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_119635_length_1331_cov_3.449060, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000653 DAOTIP010000000
VERSION     DAOTIP010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119635_length_1331_cov_3.449060"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5138_10850"
     CDS             complement(<1..264)
                     /locus_tag="P5138_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61120.1"
                     /translation="MVVPFGIVCCASGKFDGAGGGRDHPWSINRQGANEPVPAQVTRL
                     KGRIVTVGEDLDTICEFRVADVLEAGSVLVAPEAREPGRRTALP"
     gene            263..469
                     /locus_tag="P5138_10855"
     CDS             263..469
                     /locus_tag="P5138_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRV61121.1"
                     /translation="MKYRAIVDENSCCGHGDCVDAAPSVFRLDDDLAEVMGNGPLELL
                     LDAAEACPAGAISVIDESGEQIYP"
     gene            complement(550..623)
                     /locus_tag="P5138_10860"
     tRNA            complement(550..623)
                     /locus_tag="P5138_10860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(587..589),aa:Ile,seq:cat)
     gene            complement(646..1008)
                     /locus_tag="P5138_10865"
     CDS             complement(646..1008)
                     /locus_tag="P5138_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRV61122.1"
                     /translation="MSEEKSDNELLSAFAKEARQRCDVISAGLDTGTTDFETLRQEAH
                     ALHGTASTLGLRRIGELAGLMESDLSDAAKAGEIRPNRASQISEAAEALASGVKAAAD
                     GEEEPPDVGRSLSQLFAL"
     gene            1124..>1331
                     /locus_tag="P5138_10870"
     CDS             1124..>1331
                     /locus_tag="P5138_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV61123.1"
                     /translation="MDFILRNGARVAKRAALVTGGSGGIGLAIARRLAESGYAVTISG
                     RREDKLLKAAEGLANDDLKVEAVVA"
BASE COUNT          235 a          424 c          440 g          232 t
ORIGIN      
        1 ggggagagca gtccgccgcc cgggctcccg agcttctgga gcgaccagca cggatcccgc
       61 ctccagtacg tcggccacgc ggaactcgca gatcgtgtcg agatcctcac cgacggtgac
      121 gatcctgccc ttcaagcgag ttacctgcgc ggggaccggc tcgttggcgc cctggcgatt
      181 gatgctccac gggtgatcgc ggccgccgcc cgcaccatcg aactttccgc ttgcacaaca
      241 gacgatgccg aaaggaacaa ccatgaagta cagagcaata gttgacgaaa acagttgctg
      301 cggacacggc gattgcgtgg atgccgcacc gtcggtcttc aggcttgacg acgacctggc
      361 cgaggtgatg ggcaacgggc cgctggaact cctgctcgat gccgccgaag catgccccgc
      421 cggtgcgatc tccgtgatcg acgaatcagg cgagcagatc tacccctgaa ggtcaggccg
      481 ggcgacccct gtccctcaac tttcccggga acgggtctgt tcccgggaaa ggtctggccg
      541 gagccggaat gggccgggaa ggactcgaac cttcgaccta cggattatga gtccgctgct
      601 ctaaccaact gagctaccgg cccgggacgc tctgggagcg aatgctcaga gagcgaagag
      661 ctgggagagc gagcggccga catcgggcgg ctcttcctct ccgtctgcgg ccgccttcac
      721 cccggaagcc agggcctcgg cggcctcgga tatctggctg gcccggttcg gccgtatctc
      781 gccggccttg gcggcgtcgg agaggtcgga ttccatcaat cccgcgagct cgccgatgcg
      841 gcgcaggccc agggtcgagg cggtgccatg gagcgcgtgg gcttcctggc ggagggtctc
      901 gaagtcggtc gtgccggtgt cgagaccggc cgagatgacg tcacaacgct gcctggcttc
      961 cttggcgaaa gcgctgagca gctcgttgtc tgacttctcc tcactcatgt ctcacctcgc
     1021 aggtttcgga cgaaaacttc ggtcacgcgc tcccattctt ccctttttgg gaaggccgtg
     1081 catgctcgcc tcccggctca gccgcttctg gcataggctc ccgatggatt tcatcctacg
     1141 gaacggagcg agagtggcaa agcgagcggc attggtaacg ggaggttctg gcgggatcgg
     1201 actggcgatc gcaaggcgcc tggccgagag cggatacgcg gtcaccatct cgggccgccg
     1261 ggaagacaag ctgctcaagg ccgccgaagg tctggccaac gatgacctca aggtcgaggc
     1321 cgtggtcgcc g
//
LOCUS       DAOTIP010000654         1327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_120228_length_1327_cov_3.017296, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000654 DAOTIP010000000
VERSION     DAOTIP010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120228_length_1327_cov_3.017296"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="P5138_10875"
     CDS             complement(<1..1156)
                     /locus_tag="P5138_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRV61124.1"
                     /translation="MPEENPSLFLIDGNSMAYRAFFALPDSMATADGRPTNAIFGLAS
                     MLVKLIADYRPRQIAVAWDAGMSGREVVYPEYKSHRPPKPDLLRQQWPQLLEMVEAFG
                     YTNVKVDGYEADDVIATLTRQASAVDIPVVVVTGDRDAYQLVTDTVQVMSTSKGVTET
                     KLYDREAVKERYGVYPELVTDLMGLRGDTSDNIPGVPGIGEKTAAQLLDQFGSLERIL
                     ASVDEISGKKRKENLTEFAEDARISKQLATMDFEVETGVDIDELMAQVPDREGLRAFM
                     TEFELRAAMKRLEEALGDEEIPGVSEEAPAPAEVEVSTGGIEDLADGPIAVELYGGWA
                     ATDGKKVVGGEASAEEFADMVQGKPLIAHDVKAVGGERVGLLAALEATGKG"
BASE COUNT          198 a          457 c          422 g          250 t
ORIGIN      
        1 ggcccttgcc ggttgcttcg agcgcagcca ggagtccgac tcgctcaccg ccgaccgcct
       61 tcacgtcgtg ggcgatcagc ggcttgccct gaaccatgtc cgcgaactct tcggccgagg
      121 cctcgccgcc gaccactttc tttccatcgg ttgccgccca gcccccgtag agctcgaccg
      181 cgatcggacc gtcggccagg tcctcgatgc cgcccgtcga aacctcgacc tcggccgggg
      241 ccggagcctc ctccgagacg cccgggatct cctcgtcgcc gagcgcctcc tccagccgtt
      301 tcatggcggc gcggagctcg aattcggtca tgaaggcccg cagaccctcg cggtcgggaa
      361 cctgcgccat caactcgtcg atgtcgaccc cggtctccac ctcgaagtcc atggtcgcga
      421 gctgcttcga gatgcgggcg tcctcggcga attcggtcag gttctccttg cgcttcttgc
      481 cggatatctc gtccacgctg gcgaggatcc tctccagcga gccgaactgg tcgagcagct
      541 gggcggcggt cttctcgccg attccgggaa cgcccggaat gttgtcggag gtgtcgccgc
      601 gcaggcccat caggtcggtc acgagttccg ggtacacgcc gtagcgttcc ttgaccgcct
      661 cacggtcgta gagcttggtc tcggtcacgc ccttcgaggt gctcatcacc tgaaccgtgt
      721 cggtgaccag ctgataggcg tcgcggtcac cggtgacaac cacgacgggg atgtccacgg
      781 cgctggcctg ccgggtcagg gtcgcgatca cgtcgtcggc ttcgtagccg tccaccttga
      841 cgttcgtgta tccgaaggcc tcgaccatct cgagcagttg cggccactgc tggcggagca
      901 ggtccggttt cgggggacgg tgtgacttgt actcggggta gacgacttcc cggcccgaca
      961 tgccggcgtc ccaggccacg gcgatctgtc gcggccggta gtcggcgatc agcttcacca
     1021 gcatcgaggc gagcccgaag atcgcgttgg tcggacgtcc gtccgccgtc gccatcgagt
     1081 ccggcagggc gaagaaggcc cggtaggcca tgctgttgcc gtcgatcagg aaaagggaag
     1141 ggttctcttc gggcaccgga cgatcatagg tgctggagca gtcgggaccg gccctctcac
     1201 tggaccctca tatgatggtc cgatgccacc cgtttccgcc cagtacagcg tcaccgtccg
     1261 cgtcgaacta gatgcccgtc aggagcctct cggcaagctg accgcgaggc tcctgacggg
     1321 catctag
//
LOCUS       DAOTIP010000655         1326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_120394_length_1326_cov_2.350118, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000655 DAOTIP010000000
VERSION     DAOTIP010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120394_length_1326_cov_2.350118"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1169
                     /locus_tag="P5138_10880"
     CDS             201..1169
                     /locus_tag="P5138_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRV61125.1"
                     /translation="MLAIRVNDFGDPSVLSLEEVPDPVAGAGQTLIEVDTAGVNYGDL
                     LVRRGEYFGRKALPVIPGWEVVGSAATPDPDGAFEAGDRVVALLDGRGYARKVAAPTR
                     DVVPVPDGVSDKVALAMIIQGATAWRLLEDMKPDQRVLISGAGSGVTHLVVQLARLRG
                     AAEVAVVSSGGEKSNRVLDLGADLVVRSDGDETLKERLTEGLGGRKFDLAIDMIGGET
                     LQAMLRQLRPGGRAVIYGVAGGEPAKVNTGALLKNGLTVYGLWLGHSDGWSLREIMGR
                     LFELVEAGQLAPTLGPAFPLARADDAHRAVEARTGVGKVVLDCREV"
BASE COUNT          206 a          406 c          488 g          226 t
ORIGIN      
        1 ggtcctggtt cggaccggca agtacgaggc ggagaaggtc gccgagtcag ggatcgagcc
       61 caccgcgacc atcgactcga tcaacgacct gcccgggttg ctcgcctgag ggaatccggg
      121 cggtccttcg cccggatccg gttggtcctt cgcccggatc cggttggtcc cggggcatcc
      181 gggaatgtag cctcgtgcct gtgctggcca ttcgagtcaa cgatttcggc gatccatcgg
      241 tcctgagcct cgaggaggtg ccagatccgg tcgccggagc cggccagacc ctgatcgagg
      301 tcgatacggc cggtgtgaac tacggcgacc tgctggtgcg aaggggcgag tacttcggcc
      361 gcaaggcgct gccagtgatc cccgggtggg aagtcgtcgg gtcggcggcc acgccagatc
      421 cggacggagc cttcgaagcc ggtgatcggg tggtggcgct gctcgacggg cggggctatg
      481 cccggaaggt ggccgcaccg accagggatg tggtgccggt gccagacggg gtttccgaca
      541 aggtcgcgct ggcgatgatc atccaggggg ccaccgcctg gcggctcctg gaagacatga
      601 agccggacca gagggtcctg atcagcgggg ccggcagcgg ggtgactcat ctggtggtcc
      661 agctggcccg ccttcgtggc gcggccgagg tcgccgtcgt ctcaagcggc ggcgaaaaat
      721 ccaatcgtgt gctggacctc ggcgccgatc tggtcgttcg gtccgacgga gacgaaaccc
      781 tgaaggaacg cctgaccgaa ggacttggtg gcaggaaatt cgacctcgcg atcgacatga
      841 tcggcggcga gaccctccag gcgatgctcc ggcagctgcg cccgggaggt cgggctgtga
      901 tctacggcgt ggccgggggc gagccggcga aagtcaacac cggcgcgctt ctcaagaacg
      961 gcctgaccgt atacgggctc tggctcggcc attcggatgg ctggtccctg cgggagatca
     1021 tgggcaggct cttcgagctg gttgaggccg gtcagcttgc cccgaccctg ggtcctgcct
     1081 tcccgctggc gcgggcggac gatgcgcatc gcgcggtcga agcccgtacc ggggttggca
     1141 aggttgtgct cgattgccgg gaagtctgac cgcgagtcgc ccggaccgct gcagcgttca
     1201 gatctcggtg gccagttcga gcagccggtc aggggagcgg tcggtcttgc cggccctggt
     1261 tcgcggaatc gcctcgaccg taaaccagcg cttgggaacc ttgtacgaag cgatccgctg
     1321 cttcag
//
LOCUS       DAOTIP010000656         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_121129_length_1321_cov_2.063981, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000656 DAOTIP010000000
VERSION     DAOTIP010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121129_length_1321_cov_2.063981"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>1321)
                     /gene="thrC"
                     /locus_tag="P5138_10885"
     CDS             complement(157..>1321)
                     /gene="thrC"
                     /locus_tag="P5138_10885"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRV61126.1"
                     /translation="AECFGPLEVKYDFSELNPEETRRRIQAGSRGIWRYADFLPFEGK
                     AGDPLEPGMTPLIKADRLSEKLGTGAEIYIKNDAANPTHSFKDRVVAEAIAKAKELGF
                     ETVACASTGNLANAVAAHAAAAGLDSYVFVPSNLEEQKLLATDIYGTNLVGVKGNYDD
                     VNRLCTQLADEKPWAFVNVNLRPYYAEGSKTLAYETVEQLGWNLPDRVVAPIASGSLF
                     TKISRGFQEWQEVGLVDGGQPVFHGAQAEGCSPVATAFAEGTRVCRPQKPDTIAKSLA
                     IGDPADGPYAIAQANDTGGTIDAVSDDEIRAGIRLLAETTGIFTETAGGVTVGVLKKL
                     ADRGELKDGEKVVVYITGEGLKTLDANRDSFALTEIDPNPDSFETHFGQEVVA"
BASE COUNT          202 a          428 c          442 g          249 t
ORIGIN      
        1 gcagctcgcc gtcctgggtg atgcggtccc tcagatctcc gtgctcggca aatacagcgt
       61 cgagcacctc gcccacggtg gcaccctcgg cggagaggtc cgcctggccg cccgtggtgg
      121 cgcgaagctg ggtcggaatc ttgactgtga ctgccatcag gcgacgacct cctggccgaa
      181 atgggtttcg aaggaatccg gattcggatc gatctcggtc agggcaaagc tatcgcgatt
      241 ggcgtcgagg gtcttcagtc cttcaccggt gatgtagacc acgaccttct cgccgtcctt
      301 gagctcgcca cggtcggcca gtttcttcag cactccgacg gtcaccccgc cggcggtctc
      361 ggtgaagatt cccgtcgttt cggcgagcag ccggataccg gcgcggatct cgtcgtcgct
      421 gaccgcatcg atggtgccgc cggtgtcgtt cgcctgggcg atcgcgtagg gaccgtcggc
      481 cgggtcaccg atcgcgaggc tcttggcgat cgtgtccggc ttctgcggac ggcacacgcg
      541 ggtgccctcg gcgaaggccg tggcgaccgg ggaacaaccc tcggcctggg caccgtggaa
      601 gaccggctgg ccgccgtcga ccaggccgac ctcctgccac tcctggaatc cgcgcgagat
      661 ctttgtgaac agggagccgg aggcaatcgg ggcgacaacc cggtccggaa ggttccagcc
      721 gagctgctcg acggtctcgt aggcgagggt cttcgagccc tcggcgtaat acggacgcag
      781 gttcacgttc acgaaagccc agggcttttc gtccgcgagc tgggtgcaga gcctgttcac
      841 atcgtcgtag ttccccttga ccccgaccag gttcgtgccg tagatgtcgg tcgcgagcag
      901 cttctgctcc tcgaggttgg aagggacgaa gacatacgag tcgagtccgg ccgcggccgc
      961 atgggcggct accgcgttgg ccaggttgcc ggtcgaggcg caggcgacgg tttcgaatcc
     1021 gagctccttc gccttggcga tcgcctccgc gacgacccgg tccttgaagg agtgggtcgg
     1081 gttggccgca tcgttcttga tgtagatctc tgcgccggtg ccgagctttt cggaaaggcg
     1141 gtcggccttg atcagcggag tcatgcccgg ctcgagcggg tcgccggcct tgccctcgaa
     1201 gggcaggaaa tccgcatacc gccagatgcc ccgcgatccc gcctggatcc tccggcgagt
     1261 ctcctccggg ttcagctccg agaagtcgta cttgacctca agcggcccga agcactcggc
     1321 a
//
LOCUS       DAOTIP010000657         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_121337_length_1319_cov_3.406646, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000657 DAOTIP010000000
VERSION     DAOTIP010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121337_length_1319_cov_3.406646"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="P5138_10890"
     CDS             <1..284
                     /locus_tag="P5138_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61127.1"
                     /translation="VWIAQRLIQVWAERQAKVQLAAGERTRAMGAVAATSLARPWLMS
                     AVVLISCIIDRDAGLYAAALLVVLFTLNLATRGLAYLLGKDEKPEEAAR"
     gene            281..1240
                     /gene="atpB"
                     /locus_tag="P5138_10895"
     CDS             281..1240
                     /gene="atpB"
                     /locus_tag="P5138_10895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRV61128.1"
                     /translation="MSDAATHDQMPDTKTGFSTKTKVFFGIAIWLAVAIAFGLIFGSD
                     GKNEEFKPQNEFKLDPWININLGGIDLSINRAVLYLFLASILTITVMVWIARKMQQKP
                     NRVQTAIEVAYDVTRTDITRNNIDDERLATKWFPFLASLFFFIFFSNMLGYIPLPTNT
                     HETIDIFGIGIPAFALYAATANIAIPLVLTLVVWVSYHVEGIRAKGFVKYLASWLPSG
                     LEDMNPFGKAAIFLIEVVSHFVRLISLSVRLFANILAGHLLLLFMGGGLAVLLGIAAL
                     GVLTYPMAFAFYVFEVGIVATLQAFIFSILTAIYIGGATAESH"
BASE COUNT          236 a          461 c          348 g          274 t
ORIGIN      
        1 tcgtctggat cgcccagcgg ctgatccagg tctgggcgga gaggcaggcg aaggtccagc
       61 tggccgccgg tgaacgcacc cgggccatgg gtgccgtcgc cgccacttcg ctggcccgtc
      121 cctggctgat gtcggccgtc gtcctgatca gctgcatcat cgaccgcgat gccggcctct
      181 acgcggcggc gctgctggtt gtccttttca ccttgaacct cgccactcgc gggctggcct
      241 acctgctcgg caaagacgaa aagccggagg aagccgcccg atgagcgacg ctgcaactca
      301 cgaccagatg cctgacacca agacgggttt cagcaccaag accaaggtct tcttcgggat
      361 cgccatctgg ctggccgtgg ccatcgcttt cggcctgatc ttcggatctg acggcaagaa
      421 cgaggagttc aagccgcaga acgagttcaa gctcgacccc tggatcaaca tcaacctcgg
      481 cggcatcgac ctttcgatca accgggccgt cctctatctc ttccttgcct cgatcctgac
      541 catcaccgtc atggtctgga tcgcccgcaa aatgcagcag aagccgaacc gcgtccagac
      601 cgcgatcgag gttgcctacg acgtgaccag gaccgacatc acccgcaaca acatcgacga
      661 cgaacggctg gccaccaagt ggttcccgtt cctcgccagt ctctttttct tcatcttctt
      721 ctcgaacatg ctcggctaca tcccgctgcc gaccaacacc cacgagacga tcgacatctt
      781 cggaatcggc attcccgctt tcgccctgta cgcggcgacc gcgaacatcg ccattccgct
      841 ggtcctgacc ctggtcgtct gggtctcgta ccacgtcgaa ggcatccggg ccaagggttt
      901 cgtcaagtac ctggccagct ggcttccctc cgggcttgaa gacatgaacc ccttcggcaa
      961 ggcggcgatc ttcctgatcg aggtggtttc ccacttcgtc cgtctgatct cgctctccgt
     1021 ccgacttttc gccaacatcc tcgccggcca cctcctgctg cttttcatgg gcggcggcct
     1081 ggcagttctg ctcggtatcg ccgcactcgg tgtcctcacc tacccgatgg ccttcgcctt
     1141 ctacgtattc gaggtcggca tcgtggccac cctgcaggca ttcatctttt ccatcctgac
     1201 cgctatctac atcggcggag caaccgccga aagtcactaa aaaggagacc ctgtaatgga
     1261 tcccagttcc atcgtgcccc tcgccgaagt caccggtgat gtcactgacg ccggcaagg
//
LOCUS       DAOTIP010000658         1318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_121477_length_1318_cov_3.573238, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000658 DAOTIP010000000
VERSION     DAOTIP010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121477_length_1318_cov_3.573238"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="P5138_10900"
     CDS             complement(<1..1289)
                     /locus_tag="P5138_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRV61129.1"
                     /translation="MNWFHRAEQRYVEYGSSLEATRAFGLFRSLHDLVYNMNLSHLSP
                     SVETVFETLDDADVRTASTPFLIYRGRHRHEVNLEGITGRAIEAGLLKFDYGTWGPRE
                     LFYGDLYTSMETKCKPSSIPGKRDMYSACCGAELISRDAFDFMLLAFPDNDNYSHRHG
                     PLASVRSIERADYCMAEVIRAAGGVEPFLSDHALILLADHAHTAVEDTYDVIEWMSRK
                     WKVLPPSSEEPEEAEVAVSPTSRAAHIYLLAEQDNPVRHRRIREHLFGLDGTELVAWT
                     ERDGEPVARSTRPGHLEPGDVAVVERAGRRLDFFPLETETEGGRETVIDRRGRRWSVS
                     GNIEALGASVEDGEISMPDYPDGLGRLWSALMSPHGGDIIVSAALGYECVDWGGAAHV
                     PGGSHGSLRKEDSEGPLLFVGCGPDSPDEETAASDEQ"
BASE COUNT          211 a          427 c          447 g          233 t
ORIGIN      
        1 tgctgttcgt cggatgcggc ggtttcttcg tccggtgaat cggggccgca tccgacgaac
       61 agcagcggtc cctcggaatc ctccttgcga agcgagccgt ggctgccacc cggcacatgg
      121 gccgccccgc cccaatccac gcattcgtag ccgagcgcgg ccgagacgat gatgtctccg
      181 ccgtggggtg acatcagggc cgaccagagc cggccgagcc cgtccgggta gtcgggcatc
      241 gaaatctccc catcctcgac cgaagctccc agtgcctcga tgttgccgct gacactccag
      301 cgtcggccgc gcctgtcgat caccgtttca cggcccccct ccgtctcggt ttccagcggg
      361 aagaagtcca ggcggcggcc ggccctttcc accactgcga cgtcaccagg ttccaggtgc
      421 ccgggccggg tcgaacgggc taccggttcg ccgtccctct cggtccaggc gacgagttcg
      481 gtgccgtcca agccgaagag gtgctcgcgg atgcgccggt gccggaccgg gttgtcctgc
      541 tcggccagca ggtagatgtg ggcggcgcgg gaggtcgggc taaccgcgac ttccgcctcc
      601 tcgggttctt cggaagatgg tggaagcacc ttccacttgc ggctcatcca ctcgatcacg
      661 tcgtaggtgt cctcgaccgc ggtgtgggca tggtccgcga gcaggatcag cgcgtggtcg
      721 ctcaggaaag gctccactcc tccggccgcc cggatcacct ccgccatgca gtagtcggcc
      781 ctctcgatcg accggaccga ggccagcggc ccgtgacggt gggaatagtt gtcgttgtcg
      841 gggaaggcga gcagcatgaa gtcgaaggca tcccgggaaa tcagctcggc gccgcagcag
      901 gccgagtaca tgtcccgctt gccgggaatc gaactcggct tgcacttggt ttccatcgag
      961 gtgtagaggt cgccgtagaa gagctcgcgc ggaccccagg tgccgtaatc gaacttgagc
     1021 aggccggcct cgatcgccct tccggtgatg ccctcgaggt tcacctcgtg gcggtgccgg
     1081 ccccgataga tgaggaaggg ggtcgaagcg gtgcggacat cggcgtcatc gagagtctcg
     1141 aagacggttt cgaccgaggg gctcaggtgg ctgaggttca tgttgtagac gaggtcatgg
     1201 agcgagcgga agaggccgaa ggcccgggtt gcctccagcg aggagccgta ctcgacatac
     1261 ctctgctcgg cccggtggaa ccagttcatg ccgctgatcc agtgccggtc ggcctgga
//
LOCUS       DAOTIP010000659         1318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_121489_length_1318_cov_3.239905, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000659 DAOTIP010000000
VERSION     DAOTIP010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121489_length_1318_cov_3.239905"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="P5138_10905"
     CDS             <1..301
                     /locus_tag="P5138_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein MoeB"
                     /protein_id="HRV61130.1"
                     /translation="TPPPPELAPSCSANGVLGVMAGQMGLLQANEVLKLVLGIGEPLV
                     GRLLLYEALGTRFTELKVRRDPECPICGPNAPKVPESEMGQFPDYEAFCGGHVGS"
     gene            357..632
                     /locus_tag="P5138_10910"
     CDS             357..632
                     /locus_tag="P5138_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HRV61131.1"
                     /translation="MPSVKIPPVLRKHTGDATEVDAAGSTVGEVLNALVAEYPETGEQ
                     LFSGEGELNRFVNIYLNDEDVRVLDGLDTTVGESDTVMILPAMAGGQ"
     gene            complement(673..945)
                     /locus_tag="P5138_10915"
     CDS             complement(673..945)
                     /locus_tag="P5138_10915"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRV61132.1"
                     /translation="MSIFRRSERREKSPEPVKIAWASNVAEADLIADILRQEGIPSLI
                     KRNKGFDVPDFLAAGARDIFVPAAAAERAKEILDDLQSDQDLLPGS"
     gene            complement(923..>1318)
                     /locus_tag="P5138_10920"
     CDS             complement(923..>1318)
                     /locus_tag="P5138_10920"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRV61133.1"
                     /translation="KVPQALLDEMIEHSKADSPNECCGLIGGTGDDAITIHRMENTAH
                     SPLRYEMDSKEQLKVYKEILENGHDIVGIYHSHTRTAAYPSQTDINLASGWPDAVYFI
                     VSLENPDEPYVRGFNIRDGQVEDVDIQAV"
BASE COUNT          211 a          423 c          443 g          241 t
ORIGIN      
        1 gaccccgccg ccgccggagc tcgccccgag ctgctcggcc aacggcgtgc tcggcgtgat
       61 ggccggccag atgggcctgc tccaggcgaa cgaggtcctg aagctggtgc tcggcatcgg
      121 cgagccgctg gtcggccgcc tgctgctcta cgaagcactc ggaacccgct tcaccgaact
      181 caaggtccgg cgcgatccgg aatgcccgat ctgcggtccg aacgcgccca aggtcccgga
      241 atccgagatg ggccagttcc ccgactacga ggcattctgc ggcggccacg tcggctcctg
      301 aacggggtcg ttcgcgcact cgccgaagtc ccgttgcgat catctaggat cagcccatgc
      361 cgtccgtgaa gattccgccc gttctccgca agcacaccgg tgatgcgacc gaggtcgacg
      421 ccgccggttc gaccgtcggg gaggtgctga acgccctcgt ggccgagtac cccgagaccg
      481 gagaacagct cttctcgggc gaaggcgaac tcaaccgctt cgtcaacatc tatctcaacg
      541 acgaagacgt ccgggtgctc gacggtctcg atacgaccgt cggcgagtcc gacaccgtga
      601 tgatcctccc ggcgatggcc gggggccagt agtaaccgca ggtcgggcgg gatctgaccc
      661 gccctcaagg cttcaggatc cgggcaggag atcctggtcg gactggaggt cgtcgagaat
      721 ctccttggct ctttcggcgg cggcggccgg gacgaagatg tcgcgagccc cggcggcgag
      781 gaagtcgggg acgtcgaagc ccttgttgcg cttgatcagg ctggggatgc cttcctgacg
      841 caggatgtca gcgatcaggt cggcctcggc cacattgctg gcccaggcaa tcttgaccgg
      901 ctcgggcgat ttctcgcgcc gctcagaccg cctgaatatc gacatcctcg acctggccgt
      961 cacggatgtt gaatccccgc acgtacggct cgtcggggtt ctccagggag acgatgaagt
     1021 agacggcgtc gggccagccg gaggccaggt tgatgtcggt ctgcgagggg taggccgcgg
     1081 tccgggtatg cgagtggtag atgccgacga tgtcgtggcc gttctcgagg atctccttgt
     1141 agaccttgag ttgctccttc gagtccatct cgtagcggag cgggctgtgg gccgtgttct
     1201 ccatccggtg gatggtgatc gcgtcgtcgc ccgtcccgcc gatcaggccg cagcactcat
     1261 tcggggagtc agccttggag tgctcgatca tttcgtcgag cagcgcctgc ggaacctt
//
LOCUS       DAOTIP010000660         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_121721_length_1316_cov_3.628866, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000660 DAOTIP010000000
VERSION     DAOTIP010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121721_length_1316_cov_3.628866"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="P5138_10925"
     CDS             <1..398
                     /locus_tag="P5138_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61134.1"
                     /translation="LRESTAAWEAAKVLHGDSVEIQGRTFFGLGGGIPVTPWGWSYDL
                     EETEAEAALAELPKEAVLVVHSPPKGHCDEAGNGMSLGSTAILAAIEEHQPVLAVCGH
                     IHEAWGSESTVGTTRIVNLGPTGTYFTLD"
     gene            complement(430..>1316)
                     /locus_tag="P5138_10930"
     CDS             complement(430..>1316)
                     /locus_tag="P5138_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV61135.1"
                     /translation="IEETVAEDPASAAAEERLRAKVAEIKATQQTPAPEPAVESALAE
                     PESPAVEDTDLSPGLAAVERRLSENSEERDRALRDAEERLRRVEQRAEDAERRAAFAE
                     RLAQLKVEESEREKRLNDVVSGIDRAEQRAREAEARAEAAERAAAAALEQNEPAPPPP
                     PAPPRHASAEPSPMFAPTTPPPPEPPAAEPESAEAQADDSEASEPTPPERKGLFGGTP
                     ASSTGTSTSADASSDAVNLNSATFEELREVGLSVTQATRILAYRERFGGYGSVEDLEK
                     VPGFPADLIESLQGRITI"
BASE COUNT          186 a          446 c          460 g          224 t
ORIGIN      
        1 ccctcaggga gtccacggcg gcgtgggaag cggccaaggt cctgcacgga gattcggtcg
       61 agatccaggg gcggacgttc ttcggcctcg gtggaggcat cccggtcact ccctggggct
      121 ggagctacga cctcgaagag accgaagcgg aagctgcgct ggctgaactc cccaaggaag
      181 ccgtgctggt cgtgcactcg cccccgaaag gacattgcga cgaggccggg aacgggatga
      241 gcctcggttc aaccgcgatt ctcgcggcga tcgaagaaca tcagccggtg ctggccgtct
      301 gcgggcacat tcacgaggca tggggcagcg aatccacggt cggcacgacc aggatcgtca
      361 acctcggccc gaccggaacc tacttcacgc tggactagcc gctcgacgga gccggcccga
      421 ccatcccggt cagatcgtga tgcggccctg gaggctttcg atcaggtcgg ccgggaagcc
      481 gggcaccttc tcgaggtctt cgaccgagcc gtagccaccg aaccgttccc ggtaggcgag
      541 tatccgggtt gcctgggtta ccgagaggcc gacctcgcgg agttcctcga aggtggcgct
      601 gttgaggttc accgcatccg acgaagcgtc ggccgaagtc gaagtgccgg tcgaggaagc
      661 cggggttccg ccgaagagac cctttcgctc gggaggggtc ggctccgaag cctcggaatc
      721 gtccgcctgg gcctcagccg actccggctc cgcggcggga ggttccggtg gcggcggcgt
      781 ggtcggggcg aacatcggag acggttctgc cgacgcatgt ctcggaggtg cgggaggcgg
      841 cggcggagcg ggctcgttct gctcgagcgc ggccgcggcg gcccgttctg ccgcttccgc
      901 ccgggcctct gcctcccggg cgcgctgctc ggcccggtcg attccgctga ccacatcgtt
      961 caggcgcttc tcgcgctcgg actcttcgac cttgagctgg gccagacgct cggcgaacgc
     1021 cgcgcgtcgc tcggcgtcct cggccctctg ttcgacccgt cgcagccttt cctcggcatc
     1081 gcgcagggca cggtcacgct cctcgctgtt ctcgctcagc cgccgttcga ccgcggccag
     1141 gccgggtgaa agatccgtgt cttctacggc agggctttcc ggttcggcca gtgccgactc
     1201 gacggccggt tcaggcgccg gagtctgctg ggttgccttg atctcggcga ccttggcacg
     1261 aagccgctct tcggcggcag ccgaggccgg gtcctccgca accgtctcct cgatcg
//
LOCUS       DAOTIP010000661         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_122447_length_1311_cov_3.066083, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000661 DAOTIP010000000
VERSION     DAOTIP010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122447_length_1311_cov_3.066083"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1311)
                     /gene="uvrC"
                     /locus_tag="P5138_10935"
     CDS             complement(<1..>1311)
                     /gene="uvrC"
                     /locus_tag="P5138_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRV61136.1"
                     /translation="LDEDYPRVYFTRERHRPGRAYFGPFSSARRVRDTLDLLGKLFQY
                     RTCEGSEPGRRSGSPCLDYFIKRCGAPCVDYISRDEYRANIDRVIDFLSGRYDQIERD
                     LESRMKEASAGQDFEAAAVFRDRLASVQSLFERQRISGSTVGTADLIGVATEGTDANA
                     QVFQVRDGILAERQSFYLENPSGREIDEVAAEFIEQYYGASPAVPRRVVLGPYMSGRI
                     GEIETFLADRRGGRGVEVRVAERGDTRRLREMAERNAKLALDQDRLQSEHRQQRRVEA
                     LSELGDALGMEELPVRIEGFDISNLGGEHTVASMVVFEGGRPKKADYRRFRVHGEEGH
                     QGPDDFASMEEVLGRRIAQYRQQVDLSPHDRDRDESFASLPSVIMIDGGKGQLAAGVR
                     ALSEFIELGVTVVSLAKREEEVFTPGSSLPLELKGDSEANLLLQ"
BASE COUNT          205 a          448 c          430 g          228 t
ORIGIN      
        1 gctggaggag caggttcgcc tcggagtcgc ccttcagctc gagcggaagc gaggaacccg
       61 gggtgaacac ctcttcctcc ctcttggcga ggctgaccac cgtcactccg agctcgatga
      121 actcgctcag cgcgcgaaca ccggccgcga gctgcccctt gccgccgtcg atcatgatca
      181 cgctcggcag cgaagcgaag ctctcgtccc ggtcccggtc atgcggggaa aggtcaacct
      241 gttgccggta ctgggcgatc ctccggccga gcacctcctc catcgaggcg aagtcgtccg
      301 ggccctgatg gccttcctca ccgtggaccc ggaagcggcg gtagtcggcc ttcttcgggc
      361 gcccgccttc gaagacgacc atggaggcga ccgtgtgctc gccgccgagg ttcgaaatgt
      421 cgaaaccctc gatccgaacc ggcagctctt ccatgccgag cgcatcgccg agctcgctca
      481 gggcttcaac ccgtctttgt tgccggtgct cgctctgcag ccggtcctgg tcgagtgcca
      541 gcttcgcgtt ccgttcggcc atctcgcgca gccggcgggt gtcaccccgc tcggccaccc
      601 tcacctccac cccgcgcccg ccccggcggt cggcgaggaa ggtctcgatt tcgccgatcc
      661 gtccggacat gtacgggcca aggacgaccc gtctggggac cgcgggagag gcgccgtaat
      721 actgctcgat gaactcggca gcgacctcgt cgatctcccg cccggaagga ttctccaggt
      781 agaaggactg gcgctcggcc aggatcccgt ccctgacctg gaagacctgg gcgttggcgt
      841 cggtcccttc tgtagcgacc ccgatcaggt cggcggtacc gaccgtggag ccggagattc
      901 gctgcctttc gaaaagcgac tggacggagg ccagccggtc gcggaagacc gcggcggcct
      961 cgaagtcctg gccggccgag gcttccttca tgcggctttc gaggtcgcgc tcgatctggt
     1021 cgtacctgcc ggacaggaag tcgatcaccc ggtcgatgtt cgcccggtat tcgtcccggc
     1081 ttatgtagtc gacgcagggc gccccgcaac gcttgatgaa gtagtcgagg cagggactgc
     1141 cggagcggcg acccggctcg ctgccctcgc aagttctgta ctggaagagc ttgccgagca
     1201 ggtcgagcgt gtcgcgaacc cggcgggccg aggagaaggg tccgaagtag gcccttccgg
     1261 gacggtgccg ttcccgggtg aaatagaccc gcgggtaatc ctcgtcgagg c
//
LOCUS       DAOTIP010000662         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_122457_length_1311_cov_2.907643, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000662 DAOTIP010000000
VERSION     DAOTIP010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122457_length_1311_cov_2.907643"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="P5138_10940"
     CDS             complement(<1..313)
                     /locus_tag="P5138_10940"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRV61137.1"
                     /translation="MKWGVLQFPGSCDERDALNACQIAMTALSFGGGSGEAPSSARNV
                     VGDLTGPASLPRLVWHEETDLSDLDGVVVPGGFSYGDYLRAGAIARFSPAMTAVAEFA
                     AA"
     gene            complement(310..540)
                     /gene="purS"
                     /locus_tag="P5138_10945"
     CDS             complement(310..540)
                     /gene="purS"
                     /locus_tag="P5138_10945"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRV61138.1"
                     /translation="MIQARVLIRPKEGILDAQGKAVEGALPALGFSGIEHVRIGRIVE
                     LQADSTDQLEELCEKLLANPLIEDYEIETISG"
     gene            complement(537..>1311)
                     /locus_tag="P5138_10950"
     CDS             complement(537..>1311)
                     /locus_tag="P5138_10950"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRV61139.1"
                     /translation="LLVASDRISIYDVIMPTAIPDKGKVLTQMSNFWFENTTGIVPNH
                     FISNDVPEEVAGRAVRVRKLEMYPVECVVRGYLSGSGWKEYQQTGAVCGVGLPEGLKE
                     SDKLPRPIFTPATKAEMGDHDENVDFDRAAEIVGDRHLMEELRRIAIEIYEYGANHAA
                     EKGIILADTKFEFGSYAGAEIVLGDEVLTPDSSRYWPADQYEPGKSQPSFDKQYVRDW
                     ANAAGWDHNPPAPELPEDVVAQTQAKYREAYERITGREL"
BASE COUNT          210 a          425 c          439 g          237 t
ORIGIN      
        1 cggcggccgc gaattcagcc accgcagtca tcgcgggaga gaaacgggca atggccccgg
       61 cccgcaggta atccccatag gagaatccac cgggcacgac gactccatcc aggtccgaaa
      121 ggtcagtctc ttcatgccag accagccgcg gtagagaggc gggtccggtg aggtccccga
      181 cgacgtttcg ggccgaggag ggggcctcgc cggacccgcc tccgaagctc aaggccgtca
      241 tggcgatctg gcaggcgttg agggcgtccc gctcgtcgca ggaccccggg aactggagga
      301 cgccccactt catccgctga tggtctcgat ctcgtagtcc tcgatcagcg gattggcgag
      361 cagcttctcg cagagctcct cgagctgatc ggtgctgtcg gcctggagtt cgacgatccg
      421 gccgatccgg acatgttcga tccccgagaa accgagggcg ggcagggcac cttcaaccgc
      481 cttgccctgg gcgtcgagga tgccctcctt gggccggatc agaacccggg cctggatcaa
      541 agttccctcc cggtgatccg ttcgtaggcc tcgcggtact tggcctgggt ctgggcgacg
      601 acgtcttccg gcagctccgg cgcgggcggg ttgtgatccc agccggccgc gttggcccag
      661 tcgcgcacgt actgcttgtc gaaggaaggc tgcgacttgc cgggctcgta ctggtcggcc
      721 ggccagtagc gggaggagtc gggggtgagg acctcgtcgc cgagcacgat ctcggccccg
      781 gcgtagctgc cgaactcgaa cttggtgtcg gcgaggatga tgcccttctc ggcggcgtgg
      841 ttggccccgt actcgtagat ctcgatcgcg atccgccgca gttcctccat gaggtgccgg
      901 tcaccgacga tctcggctgc ccggtcgaag tccacgttct cgtcgtggtc gcccatctcc
      961 gccttggtgg cgggggtgaa gatcgggcgt ggaagcttgt cggactcctt gagtccctcg
     1021 ggcagcccga ccccgcagac cgccccggtc tgctggtact ccttccagcc cgagccggac
     1081 aggtaaccgc ggaccacgca ttcgaccggg tacatctcca gtttccgcac ccggacggca
     1141 cgtccggcga cctcttcggg cacgtcattc gagatgaaat ggttcggaac gatccccgtg
     1201 gtgttctcga accagaagtt cgacatctgg gtgagaacct tgcccttgtc cgggatcgcg
     1261 gtaggcatga tcacgtcgta gatcgagatc cggtcggaag cgacgaggag g
//
LOCUS       DAOTIP010000663         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_122714_length_1309_cov_4.413078, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000663 DAOTIP010000000
VERSION     DAOTIP010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122714_length_1309_cov_4.413078"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="P5138_10955"
     CDS             <1..256
                     /locus_tag="P5138_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRV61140.1"
                     /translation="GLVSEVVAPDELMARAREKADKLAQKSPYYVKMAKHLVHQSLDN
                     SITEQLQLERHGIANGMGTQDSKNAIEAFLSGGTAEFKGE"
     gene            complement(301..786)
                     /locus_tag="P5138_10960"
     CDS             complement(301..786)
                     /locus_tag="P5138_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRV61141.1"
                     /translation="METLVIEGPEQVQELVGKQVGPTDWREVTQEMIDTFADLSGDHQ
                     WIHVEVERAANESPFGTTIAHGNLTLSMIDGFRLDLIEVKSGFSMGINYGWNKVRFPS
                     PVPAGSKVRASAELSEINDLGNGWVETVTTITVEVQTPDGQIGQKPCCVGESVTRLLK
                     A"
     gene            complement(848..>1309)
                     /locus_tag="P5138_10965"
     CDS             complement(848..>1309)
                     /locus_tag="P5138_10965"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRV61142.1"
                     /translation="GDRSAALSQAVPSITPGWVRPLVLRAAMKWNVSASLLAAQLFAE
                     SGFDPDAGNGIAFGIAAFTPAAAAEYGLDDPFDPAAAIMAQAHLMSDLLKQFGSPALA
                     LAAYNAGPGAVGSCNCIPSYPETQAYVAKILGMLGGMGAMSPAGMTIELVR"
BASE COUNT          243 a          425 c          419 g          222 t
ORIGIN      
        1 cggcctggtc tcggaggtcg tggcccccga cgaactgatg gcaagggccc gcgagaaagc
       61 cgacaagctg gcgcagaagt cgccttacta cgtgaagatg gccaagcacc tggtccacca
      121 gagcctggac aacagcatca ccgaacaact tcaactggag cgccacggca tcgccaacgg
      181 catgggcacc caggattcga agaacgcgat cgaagcgttc ctctccggcg gcaccgccga
      241 attcaagggc gaatagccgc aggcgcagcg cgggagccag tcaccggctg ctgccggtta
      301 tcaggccttg aggaggcggg tgacgctttc tcccacgcag cagggcttct ggccgatctg
      361 accgtccggg gtctgaacct cgaccgtgat cgtcgtgacg gtctcgaccc agccgttgcc
      421 gaggtcattg atctcgctca gttccgccga ggcgcgcacc ttcgagccgg ccgggaccgg
      481 cgaggggaaa cggaccttgt tccagccgta gttgatcccc atcgagaagc cggacttcac
      541 ttcgatcagg tcaaggcgga aaccgtcgat catcgaaagc gtcaggttgc cgtgggcgat
      601 agtcgtgccg aaagggctct cgttcgcggc ccgttcgact tccacgtgaa tccactggtg
      661 atcgccggag aggtcggcga aggtgtcgat catctcctgg gtgacttcgc gccagtcggt
      721 cgggccgacc tgcttgccga ccagttcctg aacctgctcc ggaccttcaa ttacgagagt
      781 ttccatgaag caacactatc gcccagtgtt gtctccccca atgccggctg actcccgtcc
      841 ggccgtgtta cctgacgagt tcgatcgtca ttccggccgg actcattgca cccatcccgc
      901 cgagcatccc gaggatcttc gcgacatagg cctgtgtttc cggataggag ggaatgcagt
      961 tgcaggatcc gaccgcgccg ggaccggcgt tgtaggccgc cagcgccagg gccggtgacc
     1021 cgaactgctt cagcaggtcg ctcatcaggt gggcctgggc catgatcgcg gcggccggat
     1081 cgaagggatc gtcgagcccg tattcggctg ccgccgccgg agtgaaggcc gcgattccga
     1141 aggcgatccc gttaccggca tccgggtcga agcccgactc ggcaaagagc tgggcggcca
     1201 gcagggaagc ggagacattc cacttcatcg ccgctcgcag caccagcggg cgcacccatc
     1261 cgggggtgat cgacggcacc gcctgcgaga gagcggccga gcggtctcc
//
LOCUS       DAOTIP010000664         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_122778_length_1309_cov_2.268740, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000664 DAOTIP010000000
VERSION     DAOTIP010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122778_length_1309_cov_2.268740"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1309
                     /gene="menD"
                     /locus_tag="P5138_10970"
     CDS             <1..>1309
                     /gene="menD"
                     /locus_tag="P5138_10970"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRV61143.1"
                     /translation="PGSRSTPLALALFREEAIETEVALDERSASFFALGAAQATGSPV
                     ALVCTSGTAAANFHPAIAEADLSAIPLIVLTADRPPELREIGAGQTIDQIKLYGDSVR
                     WFAEVGSHEADDAGLLHFRSLACRAYATATGDPRPGPVHLNFPLRDPLAPVGEPDAVT
                     ASRRLALEGRRDGPLTVVEKPTCGADRPTVNRLVAMIQGAERILILAGRQTDTSIREP
                     LARLANACSAPVLAEPTSQFRLGPHDRGAVITAYERIARRRESSLAPDLLIRLGEFPT
                     SKQLRLWMAETDPAQTVIDPGYGWYEPVRTADLIVRCDVAALLHQIENTAEQRVEGPA
                     GNRFWNAWMEAEKRERREIESSWTREGKLDAGEVHARLAGLQSDGGLIYTASSLAIRD
                     QEESIPALDADVRFLANRGANGIDGLLASGLGAARATGGDTTII"
BASE COUNT          224 a          454 c          432 g          199 t
ORIGIN      
        1 cccgggtccc ggtccacccc cctcgcactt gccctgttcc gggaagaagc gatcgagacc
       61 gaagtggcgc ttgacgagag gtcggcatcg ttcttcgcgc ttggggccgc ccaggcaacc
      121 ggatcgccgg tcgcactcgt ctgcacctcg ggtaccgctg cggccaactt tcatccggcc
      181 atcgccgaag ccgacctctc tgcgatcccg ctgatcgtcc tcaccgcaga ccgtccgccc
      241 gagctgcgcg agatcggcgc cggccagaca atcgaccaga tcaagctcta cggggactcg
      301 gtcagatggt tcgccgaggt ggggagccac gaggccgacg atgccggact gctccatttc
      361 cgctcccttg cctgtcgggc atatgcgacg gcgacggggg acccgagacc gggtccggtc
      421 catctcaact ttccattgcg cgatccgctc gctccggtcg gtgagccgga cgccgtcacc
      481 gcgagcagac gcctcgccct ggagggccgc cgggacggac ccctgaccgt cgtcgagaag
      541 cccacttgcg gcgccgaccg gccgaccgtg aacagactgg tggccatgat ccagggggcg
      601 gagcggatcc tcatcctggc cggacgacag acggacactt cgatccgtga gcccctggcg
      661 aggctagcga acgcctgcag tgccccggtg ctggcggagc cgacctccca gttccggctc
      721 ggcccgcatg accgtggtgc ggtgatcacg gcctacgaga ggatcgcgcg gcgacgggag
      781 tcgtcacttg ctccggatct cctgatccgc ctgggcgagt tcccgacctc gaagcaactc
      841 cgcctctgga tggccgaaac cgatccggcc cagaccgtga tcgatcccgg gtacggctgg
      901 tacgagccgg tccggacagc agatctgatc gtccgctgcg acgtcgcagc cctgctccac
      961 cagatcgaga acaccgccga gcaacgggtg gaaggcccgg ccggaaacag gttctggaac
     1021 gcctggatgg aagcggaaaa gcgtgaacgc cgggaaatcg agagttcatg gacccgggaa
     1081 ggaaaactgg acgcgggtga agtccacgcc cggctcgcgg ggctgcagtc cgatggtggc
     1141 ctcatctata ccgcctcgag cctggcgatc agggaccagg aagagtcgat tcccgcgctc
     1201 gatgccgacg tgcgttttct ggcgaaccgc ggcgcgaacg ggatcgacgg gcttctggca
     1261 tccggcctcg gtgccgcccg ggccaccggc ggcgacacca cgatcatca
//
LOCUS       DAOTIP010000665         1307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123056_length_1307_cov_2.598243, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000665 DAOTIP010000000
VERSION     DAOTIP010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123056_length_1307_cov_2.598243"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="P5138_10975"
     CDS             <1..498
                     /locus_tag="P5138_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRV61144.1"
                     /translation="SISTEGFETRYETELANDFGNLASRTLAMISRYRDGVQPEAEPD
                     PLLAADFDGIVDRVCRLLDGAQLSQALEEIWVLVRRLNRYVEEAQPWVLAKDEADADR
                     LDQVLYSLGEGLRVLTLLLHPWLPNSTVNLLGALGEQGNGLAAYGSRPGGAKMEKIEP
                     LFPKL"
     gene            516..941
                     /locus_tag="P5138_10980"
     CDS             516..941
                     /locus_tag="P5138_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61145.1"
                     /translation="MNKVRTVVAAIAIVAVGAAVVVPSVSAAVDPNRPLKVRVFTPDG
                     KQRLKVKRKIAVVVGCTKNCAVVVDMKLVMPGEVLKARVKGGIKAFSAKSPAMILNTP
                     AIRYLRFSYPRSRFSVKVTATDISNGKRVVKRKVFRFRR"
     gene            948..>1307
                     /locus_tag="P5138_10985"
     CDS             948..>1307
                     /locus_tag="P5138_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRV61146.1"
                     /translation="MIDSHSHVSRCPDSPESVIGAAKEAGVEKILTVGLDEASNREQI
                     ALCGAFEGLFAAIGRHPNDADGFDDAAAADIEGLAADPKVVAIGETGLDFYRDTADPD
                     NQRLAFIKQADIAARLDL"
BASE COUNT          251 a          414 c          426 g          216 t
ORIGIN      
        1 tcgatctcga ccgaaggctt cgagacccgc tacgaaaccg aactcgccaa cgatttcggg
       61 aatctggctt cccggacact cgccatgatc agccgctatc gggacggcgt ccagccggag
      121 gccgaaccgg acccgctgct ggccgccgac ttcgacggaa tcgtcgatcg cgtctgccga
      181 ctcctcgacg gggcacagct cagccaggcc ctggaggaga tctgggtcct ggtccggcgg
      241 ctcaaccgct acgtcgagga ggcccagccc tgggttctgg ccaaggacga ggcggatgcc
      301 gaccggctcg accaggtgct ttacagcctg ggagagggtc tcagggtgct gaccctcctg
      361 ctccacccct ggctgccgaa ctcgaccgtg aatctgctcg gggccctggg cgagcaggga
      421 aacggactcg ctgcttacgg aagccgcccc ggtggtgcga aaatggagaa gatcgaaccc
      481 cttttcccca agctttgaag aactaggatc ggaacatgaa caaagtcagg acagttgtcg
      541 cagcgatcgc catcgtcgcc gtcggggccg cagtagtcgt cccgtcggtc tccgcggccg
      601 tcgatcccaa ccggccgctc aaggtcaggg tcttcacgcc ggacggcaag cagcggctca
      661 aggtcaagcg caagattgcg gtggtggtcg gctgcaccaa gaactgcgcg gtggtggtcg
      721 acatgaaact ggtcatgcca ggagaagtcc tgaaagcccg ggtcaagggt ggcatcaagg
      781 ctttctcggc caaatcgccg gcgatgatcc tgaacacccc ggcgatcagg tacctgaggt
      841 tcagttaccc gcgctcccga ttctcggtca aggtcacggc aaccgacatc tccaacggca
      901 agagggtcgt caagcggaag gtcttccgct tccgccggta gtcgaacttg atcgactcgc
      961 attcgcatgt gtcgcgctgc cccgactcgc ccgagtcggt gatcggggct gcgaaggagg
     1021 ccggggtgga gaagatcctc acggtcggcc tcgacgaggc ttccaaccgc gagcagatcg
     1081 cactctgcgg cgctttcgag gggttgttcg cggcgatcgg acggcatccg aatgacgcgg
     1141 acggattcga cgatgcggca gcggccgaca tcgaagggct ggctgcggac ccgaaagtgg
     1201 tggcgatcgg cgagaccgga ttggacttct accgggacac ggccgacccc gacaaccagc
     1261 ggctggcctt catcaaacag gcggacatcg ccgcccggct cgatctg
//
LOCUS       DAOTIP010000666         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123171_length_1306_cov_3.150280, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000666 DAOTIP010000000
VERSION     DAOTIP010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123171_length_1306_cov_3.150280"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..791
                     /gene="lepB"
                     /locus_tag="P5138_10990"
     CDS             216..791
                     /gene="lepB"
                     /locus_tag="P5138_10990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRV61147.1"
                     /translation="MIVALALGLALLIQAFLIKPYQIPSGSMEPTLDINQRILVNRFI
                     YHFTDPKPGDVVVFHPPAGVDRGGVAECGAPHDADQPCPEPTSDKSSETFIKRIVAGP
                     GDTISIERGLPVVNGKVVEPDGYEINPCGPAGSGCNMPKKITVPEGHYFMMGDNRGHS
                     DDSRFWGPLPEEWLIGKAFATYWPPNRIGGL"
     gene            781..>1306
                     /locus_tag="P5138_10995"
     CDS             781..>1306
                     /locus_tag="P5138_10995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRV61148.1"
                     /translation="MASSPEGLFRHDLDLGADQVAGADEAGRGCLAGPIVAAAVLLDR
                     GCLESEGDGRLSGIRDSKKLTAKARERLYPEVLACAVRAVVVMRSARYIDENGLHVSN
                     IECLGRAIEGLDPDPGAVILVDGFALKGCQVPHRRLVKGDSTSAAVAAASIIAKVTRD
                     RCMARAGNAYAGYGF"
BASE COUNT          251 a          414 c          451 g          190 t
ORIGIN      
        1 gctgaagagg ccaccgccga ggaagctgcg gaggctccgg aagccgaggc tgccgaagcc
       61 gaagaggcac ccgaagccga ggccgctgat gaggctcccg aggctgaagc cactgaagag
      121 cccgccgaag acggcgagca gggcgagaag gaagaagcct gagcccgaag atccccaagc
      181 cgaagacggc cggggggagc ttcgtcgaac tggtcgtgat cgttgcgctg gcccttgggc
      241 tggcgctgct gatccaggca ttcctgatca agccgtacca gatcccgtcc ggctccatgg
      301 agccgaccct ggacatcaat cagcggatcc tggtcaaccg gttcatctac cacttcaccg
      361 atcccaagcc gggtgacgtc gtcgtcttcc acccgccggc cggagtcgac cgcggaggcg
      421 tcgccgaatg cggcgcgccg catgatgccg accaaccgtg cccggagccg acctcggaca
      481 agtcgagcga gactttcatc aagcggatag tcgccggacc cggagacacc atctcgatcg
      541 aacgcggact gcccgtggtg aacggcaagg tggtcgaacc ggatggctac gagatcaacc
      601 cctgcggtcc ggccggttcc ggctgcaata tgccgaagaa gatcaccgtg cccgagggtc
      661 actacttcat gatgggtgac aaccggggtc acagtgacga cagccggttc tggggcccac
      721 tcccggaaga gtggctgatc ggcaaggcct tcgccaccta ctggccgccg aatcggatcg
      781 gtggcctcta gccccgaagg gctgttccgg catgacctcg acctcggcgc cgatcaggtc
      841 gccggggccg atgaagcggg acggggctgc ctcgccgggc caatagtcgc ggcggcggtc
      901 ctgcttgacc gcggctgcct ggagagcgag ggcgacggac ggctcagcgg aatccgggat
      961 tccaagaagc tgaccgcaaa ggcaagggag cgcctctatc cggaggtgct ggcctgcgcg
     1021 gtccgggcgg tcgtggtgat gcgctcggca cgctacatcg acgagaacgg ccttcacgtc
     1081 agcaacatcg aatgtctcgg cagggccatc gagggacttg atccggatcc cggggccgtg
     1141 atcctggtcg acggcttcgc cctgaagggc tgccaggtgc cacatcgccg gctggtcaag
     1201 ggagacagca ccagcgcagc ggtggcggcg gcttcgatca tcgccaaggt aacccgcgac
     1261 aggtgcatgg caagggccgg aaatgcctac gcggggtacg gtttcg
//
LOCUS       DAOTIP010000667         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123173_length_1306_cov_3.052758, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000667 DAOTIP010000000
VERSION     DAOTIP010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123173_length_1306_cov_3.052758"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="P5138_11000"
     CDS             <1..394
                     /locus_tag="P5138_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV61149.1"
                     /translation="IWPKLLIIALICFFPIAVNTLDGLRRTDPVAVKMMRSLDASRMG
                     IFRRVEAPTALPGIFTGARIAAAVAVIGAVFGEWAGSDKGLGHLILQDNAQLMTARMF
                     ASVFVLSLIAIALYSLIGLLERAVVRWR"
     gene            445..>1306
                     /locus_tag="P5138_11005"
     CDS             445..>1306
                     /locus_tag="P5138_11005"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV61150.1"
                     /translation="MRRFALLPFAILVALAMSACGTKSEDVTPVRDPLKVAMDWYPNP
                     DHAGFLVAQKQGYFKDAGLDVSLDSPTDPSLPIKLVAAGKADLALSYEPEVLLAREQG
                     LDVVSVASVIDQPLTSMIWLKKSKIKNVKGLKGKTVSTAGIPYQDAYLKTILARAGLS
                     ENDVKKVGVGQGLLPSIVSGKAQATLGPFWNIEGIELKKEGLKPTINPVNKLGVPTYS
                     ELVLVAKGSRIQDDPDPIRLFITALSRGTRFAVSHPNKATATVLEANDALKPKITAAQ
                     MKATLPVLDQD"
BASE COUNT          226 a          447 c          409 g          224 t
ORIGIN      
        1 gatctggccg aagctgctga tcatcgccct gatctgcttc ttcccgatcg cggtcaacac
       61 cctcgacggg ctgcgccgga ccgaccccgt ggcggtcaag atgatgcgct ctctcgacgc
      121 cagccggatg gggatcttca ggcgggtcga ggcgccgact gcgctgcccg ggatcttcac
      181 cggcgcccgc atcgccgccg cggttgccgt gatcggggcc gtcttcggcg aatgggccgg
      241 ttctgacaag ggcctcggcc acctgatcct gcaggacaac gcgcagctga tgaccgcgcg
      301 catgttcgcc tccgtcttcg tgctgtcgct gatcgcaatc gccctctact cgctgatcgg
      361 gctgctcgaa cgggctgtcg tgcgttggcg gtaggccata acctctaggc tccgccggaa
      421 cctgttcacg tccctggaga tgctttgaga cgtttcgctc tgctgccctt cgcaatcctc
      481 gtcgccctgg cgatgtctgc ctgtggcacc aagtccgagg atgtcacccc ggtgcgcgac
      541 ccgctgaagg tggcgatgga ctggtacccg aacccggacc atgccgggtt cctggtggcg
      601 cagaagcagg gctatttcaa ggatgcgggc ctcgatgtct cgctcgactc gccgaccgac
      661 ccttcgctgc cgatcaagct ggtcgccgcc ggcaaggccg acctggccct ctcctacgag
      721 ccggaggtgc tcctggcccg ggagcaggga ctggacgtgg tctcggtcgc cagcgtgatc
      781 gaccagccgc tgacctcgat gatctggttg aagaagtcga agatcaagaa cgtcaagggc
      841 ctgaagggca agaccgtctc gaccgcgggg attccgtacc aggacgccta cctgaagacg
      901 atcctcgccc gggcgggcct ctccgagaac gacgtgaaga aggtcggagt cggccagggc
      961 ctgctcccct cgatcgtcag cggcaaggcc caggcgaccc tcggcccgtt ctggaacatc
     1021 gaggggatcg agctgaagaa ggaaggcctg aagccgacca taaacccggt caacaagctc
     1081 ggggtcccga cctacagcga actggtgctg gtcgccaagg gcagccggat ccaggatgac
     1141 ccggacccga tccgactctt catcaccgcg ctttcccgag gcacccgctt cgccgtctcg
     1201 catccgaaca aggcgaccgc aaccgtcctc gaagccaacg acgccctgaa gccgaagatc
     1261 acggccgccc agatgaaggc caccctgccg gtcctcgatc aggacg
//
LOCUS       DAOTIP010000668         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123186_length_1306_cov_2.760192, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000668 DAOTIP010000000
VERSION     DAOTIP010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123186_length_1306_cov_2.760192"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5138_11010"
     CDS             <1..324
                     /locus_tag="P5138_11010"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRV61151.1"
                     /translation="DGFLADNPVNRYLLNDRSHLKYNDDGSLDIYVQPDQPADPEQVS
                     NWLPSPAADAAQPGFRLTMRLYGLSKAGVKGLLDGTGWRGPSIYPCGAGNQTSTGIAC
                     AAQLG"
     gene            complement(327..1187)
                     /gene="rsgA"
                     /locus_tag="P5138_11015"
     CDS             complement(327..1187)
                     /gene="rsgA"
                     /locus_tag="P5138_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRV61152.1"
                     /translation="MVGLHRNHIVDLLTVDGELAGRPAGRMLQDRSSSTAMPAVGDWV
                     AALPDGTIQEILPRRSTLARRSAADRDRIQILATNIDKAIVISSLNREHDPVRLDRMV
                     RLAEASGAEPVIGLSKSDLTDEAHELKRRVQEAFPSVKVFVFSSRTGEGVEAVREFLA
                     PGETVVMLGRSGVGKSTLANTLLGWDRQKTAEIRGTDDRGRHATTSRELFPIPGGALL
                     IDTPGLRAPGSIEADDLIPGSAESDLTDERAREIERLAAYCRFRDCAHETEPDCAVNA
                     AIEAGELPAR"
BASE COUNT          197 a          446 c          462 g          201 t
ORIGIN      
        1 gacggctttc tggccgataa cccggtcaac cgctacctgc tcaacgaccg ctcccacctg
       61 aagtacaacg acgacggttc cctggacatc tacgtccagc ccgaccagcc cgccgacccg
      121 gaacaggtca gcaactggct gccgtcaccg gcggccgatg ccgcccagcc gggcttccgc
      181 ctgaccatgc gcctctacgg cctctcgaag gccggggtca aggggctgct cgacggaacc
      241 ggctggcggg gaccgtccat ctacccctgc ggggccggca accagacgtc aaccggaatc
      301 gcctgcgccg cccagctggg ctgaactcag cgggccggca gctcgccggc ttcgattgcg
      361 gcgttgaccg cgcagtccgg ttcggtttcg tgggcgcagt cgcgaaagcg gcagtaggcg
      421 gcgaggcgct cgatctcccg ggcacgctcg tcggtcaggt ccgattcggc cgaacccggg
      481 atcaggtcat cggcttcgat cgagccggga gcgcggaggc ccggggtgtc gatcaggagt
      541 gccccgcccg ggatcgggaa gagttcacgg ctggtggtgg cgtgtcggcc gcggtcgtcg
      601 gttccgcgga tctcggcggt cttctgtctg tcccagccga gcagggtgtt ggccaaggtc
      661 gacttgccga ccccggaacg gccgagcatc accaccgtct ccccgggggc gaggaattcc
      721 cgaaccgcct ccacgccttc gccggtccgg gaactgaaga cgaacacctt gaccgaggga
      781 aaggcctcct ggaccctccg cttgagctcg tgggcctcgt cggtcaggtc cgacttcgaa
      841 aggccgatca ccggctccgc gccggaagcc tcggccagcc ggaccatgcg atcgagccgg
      901 accgggtcat gctccctgtt aagtgagctg atcacgatcg ccttgtcgat gttggtggcg
      961 aggatctgga tccggtcgcg atcggcagcg ctgcgccggg ccagggtgct gcgccggggg
     1021 aggatctcct ggatcgtgcc gtcgggcagg gcagccaccc agtcgccgac cgccggcatc
     1081 gcggtggagg aggagcggtc ctggagcatc cggccggcgg gccggccggc cagctcgcca
     1141 tcgaccgtca gcaggtcgac gatgtggttg cggtggagtc cgaccacgcg ggccgggacg
     1201 tctggaccac ctgggcccac acctacggcg cctggtggac gccgggcgtc ctgatgccga
     1261 cgaagcgggt gttcgggaag tccctgcaga gctggtacga ggactg
//
LOCUS       DAOTIP010000669         1304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123456_length_1304_cov_3.291433, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000669 DAOTIP010000000
VERSION     DAOTIP010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123456_length_1304_cov_3.291433"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5138_11020"
     CDS             complement(<1..427)
                     /locus_tag="P5138_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61153.1"
                     /translation="MNPSLASYVAGVLQIAAIVGAVWYGSWWLRRWIVPEFSGALARL
                     AELTIGFALITISLELVGSVGLLKEAWITVACIVIPLIGAGLARRFAPQGVKPIAAPA
                     IPNWAFLVAIAVASWCVAEWTFPTQLSLDQGMFGGDTTWY"
     gene            complement(507..1166)
                     /gene="msrA"
                     /locus_tag="P5138_11025"
     CDS             complement(507..1166)
                     /gene="msrA"
                     /locus_tag="P5138_11025"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRV61154.1"
                     /translation="MFFGRDKTTMPAPEDALPGRSDYDFPIPDKHTVLGTPIKGPFPE
                     GIETAYFALGCFWGAERLFWNLDGVYTTAVGYQNGFTPYPTYEEVCTGKTGHAEAVLV
                     AFDPGKISYEKLLEVFFSEHDPTQGMRQGNDVGTQYRSAIFTVGDRQREVAEMARDTY
                     QEALKERGLGEITTGIEEAGPFYYAEGPHQQYLDKVPNGYCGLAGTGVACRVPVPGGS
                     G"
BASE COUNT          239 a          414 c          434 g          217 t
ORIGIN      
        1 ggtaccaggt ggtgtcaccg ccgaacattc cctggtccag gctcagctga gtcgggaagg
       61 tccattcggc cacgcaccac gaggccaccg cgatggcgac caggaaggcc cagttcggga
      121 tggccggcgc ggcaatcggc ttcacgccct ggggcgcgaa cctgcgggcc aggccggccc
      181 cgatcaacgg gatcacgatg cacgcgaccg tgatccaggc ctccttcaga aggccgaccg
      241 agccgaccag ctcaagtgaa atggtgatca gcgcgaagcc gatggtgagc tctgccagcc
      301 tcgcgagggc acccgagaat tcgggcacga tccatcgccg cagccaccag gagccgtacc
      361 agacggcgcc cacgatggcg gcgatctgga gtactccagc tacgtatgaa gctagggacg
      421 ggttcacgac cgtcctgtga acaggtttgg caccgcggga atcctaattt atggccgggc
      481 gagaggaccg gcccccgcga gaccggtcag ccggaaccac cgggaacggg aacccggcag
      541 gccaccccgg taccggccag accgcagtaa ccgttcggaa ccttgtccag atactgctga
      601 tgcgggccct ccgcgtagta gaaggggccg gcctcttcta tgccggtcgt tatctccccc
      661 agtccccgct ccttcagcgc ctcctggtaa gtgtcgcggg ccatttccgc gacctcgcgc
      721 tgccggtcac cgaccgtgaa gatggccgac cggtactggg tgccgacgtc attgccctgg
      781 cgcattccct gggtcgggtc atgctcggag aagaagactt cgagcagctt ctcgtagctg
      841 attttcccgg ggtcaaaagc gaccagcacc gcttcggcgt gacccgtctt gccggtgcag
      901 acttcctcgt aggtcgggta cggcgtgaaa ccgttctggt agccgacagc ggtcgtgtag
      961 acgccatcga ggttccagaa gagtcgctcc gctccccaga agcagccgag ggcaaagtag
     1021 gcggtctcga tcccttccgg gaaaggcccc tttatcgggg tgcccaggac cgtgtgtttg
     1081 tccgggatcg gaaagtcgta gtcggatcgg cccgggagcg catcctccgg ggcgggcatg
     1141 gtggtcttgt cgcggccgaa gaacatgaac cgatgctact ccggaggtat taacagtcgg
     1201 tgaatgaacg gccggtgaat cccggcagcg ggccttccgg agttcaggag ccgggagcgg
     1261 gcacctggct ggccatgtgc agatagccgg ccgcgctggg atgg
//
LOCUS       DAOTIP010000670         1304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123481_length_1304_cov_2.857486, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000670 DAOTIP010000000
VERSION     DAOTIP010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123481_length_1304_cov_2.857486"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="P5138_11030"
     CDS             complement(<1..919)
                     /locus_tag="P5138_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV61155.1"
                     /translation="MPLLEPDVSKITPEPSEPSFDRAPDWVAGGTPEPLRSQLTALLG
                     EEKILARATDLIRYASDASPYRMLPKAVVLAHSAGDVAKVLEFGRREGIPVTFRSGGT
                     SLNGQGQTEGILVDVRKHFTGVEVLDEGLKARVGPGTILGHANKVLAPYGRKLGPDPA
                     STDIATVGGVVSNNSGGMRCGVTYDSYQTVTGMTFVLPSGTMIDTEAEGAEEEFAAKE
                     PALAEGLMEIREEILADQDFVDRIRHKFEIKNTTGYRLCAFLDGETPLEIFRRLIIGS
                     EGTLAFIASVTFETVELPVVTTTAWLHFPD"
     gene            complement(954..>1304)
                     /locus_tag="P5138_11035"
     CDS             complement(954..>1304)
                     /locus_tag="P5138_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV61156.1"
                     /translation="PVRRRPAGRLRTGEGSVQYAVTAFWTAAAYEIPVTFLILRNEEY
                     GILKWFAEVEQVTGAPGLDLPALETGKIASGYGIESTTAKTAAEVEAALKEALADPKP
                     RLVEVPVEPGMALF"
BASE COUNT          200 a          425 c          436 g          243 t
ORIGIN      
        1 tgtcagggaa gtgcagccag gccgtcgtgg tcaccaccgg cagctcgacc gtctcgaaag
       61 tgacgctggc gatgaaggca agcgttcctt cggagccgat gatcaggcgg cggaaaatct
      121 cgagcggcgt ctcgccgtcc aggaaggcac aaagccgata gccggtcgtg ttcttgatct
      181 cgaacttgtg cctgatccgg tcgacaaagt cctggtcggc aaggatctcc tcgcggatct
      241 ccatcaggcc ctcggccagc gccggctcct tcgccgcaaa ctcctcctcg gctccttcgg
      301 cctcggtgtc gatcatcgtg ccggagggca gcacgaaagt catgcccgtg accgtctggt
      361 acgagtcgta ggtgaccccg caacgcatgc cgccggagtt gttgctgacc acgccgccta
      421 ccgtggcgat gtcggtcgaa gcgggatcgg gcccgagctt gcgcccgtag ggggcgagca
      481 ccttgttggc gtggccgagg atggttcccg gaccgacccg tgccttcagc ccctcgtcaa
      541 gcacctcgac tccggtgaag tgcttgcgca cgtcaaccag gattccctcg gtctggccct
      601 ggccgttgag gctggtacca ccggaccgga aggtgaccgg gatgccctcc cgtcggccga
      661 actcgagcac ctttgccacg tccccggcgc tatgggccaa gaccaccgcc ttcggcagca
      721 tccggtacgg gctcgcgtcg gaggcgtaac ggatcagatc ggtcgcccgg gcgaggatct
      781 tttcctcgcc gagcagcgcg gtgagctgcg agcgaagcgg ctccggtgtg ccgcccgcca
      841 cccagtcggg tgcacggtcg aaggaaggct cgctcggctc cggggtgatc ttcgagacat
      901 ccggttcgag cagtggcatc ggggctcccg ttcggtggtt ggtctgtgct cgcctagaag
      961 agggccattc ccggttcgac cggcacctcg accagtcgcg gcttggggtc ggcgagcgct
     1021 tccttcaggg ccgcttcgac ctcagcggcc gtctttgctg tggtgctctc gattccgtat
     1081 ccggaggcga tctttccggt ctccagggcg ggcaggtcga ggccgggggc gccggtgacc
     1141 tgttccacct cggcgaacca cttgagaatt ccgtactcct cgttgcggag gatcaggaag
     1201 gtgaccggaa tctcgtatgc ggccgcggtc cagaaagcgg tgaccgcgta ctggaccgag
     1261 ccttcgccag tacgcagacg accggccggt cttcggcgta ctgg
//
LOCUS       DAOTIP010000671         1302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_123811_length_1302_cov_1.991981, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000671 DAOTIP010000000
VERSION     DAOTIP010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123811_length_1302_cov_1.991981"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="P5138_11040"
     CDS             <1..970
                     /locus_tag="P5138_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV61157.1"
                     /translation="AFAAQIGEKIGLVWGMRTACIMIVAGNLIIAATPDFLGVAVGRI
                     LPGLGFALTTTVGVVWARHEGGVKLVGVFGAAIQMGIALSLLVGSVLFDQGVDWRVGF
                     LISASLAALAMGWIPPWAETPPTPEKKSSGFLRLAWRKARVYRLSLLFLSIYGVPMIL
                     GAWMIEYLSSEGDVSKTLAGLASFLLFGLSAAMRFGGAELRAKGWTHKVLCGSLGLAA
                     AGLAAVAIDPVAGAAFLAAVLLATGFGLPYATALDEAQDLYPEEPAEPLALMTLVALL
                     LPIVVIPLVGHAISRDAGDVAFGALAAFLVLATLANLKRSGVPLTE"
     gene            997..>1302
                     /locus_tag="P5138_11045"
     CDS             997..>1302
                     /locus_tag="P5138_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61158.1"
                     /translation="MEPNASSEAAAPRIAPGTPGDGLILNPKTWSAPHLEAADEDRLK
                     KMIEWFENRGLKQLKDDDRENRWYSDFLEMQAELGIFSTFLTPARDGEGDPARRWDTT
                     "
BASE COUNT          188 a          445 c          422 g          247 t
ORIGIN      
        1 tgccttcgcc gcccagatcg gcgagaagat cggattggtc tggggcatga ggacggcgtg
       61 catcatgatc gtggccggca atctgatcat cgccgccact cccgactttc tgggggtcgc
      121 cgtgggcagg atcctgcccg gactcggctt cgccctgacc acgacggtcg gagtcgtctg
      181 ggccaggcac gagggcgggg tgaagctggt cggggtattc ggtgccgcga tccagatggg
      241 gatagcgctt tcactcctgg tgggaagcgt cctcttcgac cagggcgtcg actggcgggt
      301 cgggttcctg atcagcgctt cgctggccgc tctcgcgatg ggctggattc ccccctgggc
      361 agagaccccg ccgaccccgg aaaagaagtc ctccggtttc ctccggctgg cctggcgcaa
      421 ggcccgggtc taccggctct cgcttctgtt cctttccatc tacggggtgc cgatgatcct
      481 cggggcctgg atgatcgaat acctctccag cgagggcgat gtctccaaga ccctggccgg
      541 cctggcctct ttcctgcttt tcggcctgtc ggcggcgatg cgcttcggcg gcgccgagtt
      601 gagagcgaaa ggctggaccc acaaggtgct ctgcggttcg ctgggcctgg ctgcggccgg
      661 ccttgccgcg gtcgcgatcg acccggtcgc cggcgccgcc ttcctcgccg cggtgctgct
      721 cgccaccggc ttcggactgc cctacgccac ggcccttgac gaggcccagg acctctaccc
      781 ggaagagccg gcagaaccgc tggccctgat gacccttgtt gccctgctcc tcccgatcgt
      841 cgtcattccc ctggtgggtc atgcgatctc ccgtgacgcc ggtgacgtcg ccttcggagc
      901 cctcgcggcc ttcctggtcc tggctacgct cgccaacctg aaacgttccg gggtccccct
      961 caccgaataa ttctccgtcg ggcatatgct cctcgcatgg agcccaacgc gagttctgaa
     1021 gctgcagccc cccggatcgc tcccggtact cccggtgacg gcctgatcct caatccgaag
     1081 acctggtcgg cgccgcatct cgaggccgcc gacgaggacc ggctgaagaa gatgatcgag
     1141 tggttcgaga accgcgggct gaagcagctc aaggacgatg accgcgagaa ccgctggtac
     1201 tcggactttc tcgagatgca ggcggaactc gggatcttca gcaccttcct cactccggcg
     1261 cgggacggtg aaggcgaccc ggccaggcga tgggacacga cc
//
LOCUS       DAOTIP010000672         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_124107_length_1300_cov_2.171084, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000672 DAOTIP010000000
VERSION     DAOTIP010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124107_length_1300_cov_2.171084"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1300)
                     /locus_tag="P5138_11050"
     CDS             complement(<1..>1300)
                     /locus_tag="P5138_11050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRV61159.1"
                     /translation="GREVTFDAPTIDYAGLSPIIALTAGLVVIVLAAVFDRVKRFGPG
                     LALLTYAVTAGCLIWQWNADLDLVSGALRIDGLAVTLSLLAVIAAAVSVLLAIGDPSE
                     TQAGRSDWLALLTGSLLGMVILAQATSMLTFFVGLELLSIPLYALCGTNLRRRESLES
                     GLKYLIVGSVGSATLLYGLAMIYGASGATGFQAIADGLQAGDGMLSDSLTLVGVGLVA
                     VGLAFKVSIAPFHQWTPDVYQGAPTPVTAFMAVATKAAAFAVFIRFFLVALGPLADQW
                     DVMLAALAALSIVVGNVGALAGDNLRLFAGFEQPCGGKQGVSLEVLRNDRPVIREVAL
                     NQLGSQFQIVHVIPQILPLESLELLVFGGSHPKRGLRNPGGKDLVILVLPGSALFPFV
                     GQFLSNRDAAHSFLDPVIAVALEFLERPGPFGGQFRILELL"
BASE COUNT          287 a          405 c          430 g          178 t
ORIGIN      
        1 aaggagctcg agaatccgga actggccacc aaacggaccc ggacgctcca aaaactcaag
       61 ggctacagcg atgactggat cgagaaacga atgcgcggca tcgcggttcg agaggaactg
      121 accgacgaat ggaaacaacg cggagcccgg gaggacaagg attacgagat ccttaccgcc
      181 gggatttcgc aggcctcgtt tggggtgact cccgccgaat acaagaagct caagggactc
      241 aagcgggaga atctgcggga tcacatggac gatctggaac tggcttccca actgattgag
      301 ggcaacttcc cggattaccg ggcgatcatt cctcaaaact tcaagactca caccctgctt
      361 tccaccgcag ggctgctcaa agcctgcaaa cagtcggaga ttatcgcccg cgagggcacc
      421 aacgttgccg accactatcg agagcgctgc cagggctgcc agcatcacat cccactggtc
      481 cgccagcgga ccgagggcaa ccaggaagaa gcggatgaac acggcgaagg ccgccgcctt
      541 ggtcgcgacc gccatgaagg cggtgaccgg ggtcggcgct ccctggtaga cgtcgggggt
      601 ccattggtgg aagggggcga tcgagacctt gaaggcgaga ccgaccgcga ccagtcccac
      661 cccgaccagg gtcagggagt ccgagagcat gccgtcgccg gcctgaaggc catcggcaat
      721 cgcctggaat ccggtcgccc ccgaggcgcc gtagatcatt gccagtccgt agagcagggt
      781 ggccgaaccg accgatccca cgatcaggta tttgagcccg gactcgagcg attcgcgtcg
      841 gcgcaggttg gtgccgcata gcgcatagag cgggatcgag agcagctcca ggccgacaaa
      901 gaaggtcagc atgctggtcg cctgggcgag gatcaccatg ccgagcagtg acccggtgag
      961 cagcgccagc cagtcgctgc ggccagcctg ggtctccgac gggtccccga tggccagaag
     1021 gaccgacacc gccgcggcga tcaccgcaag cagtgagagg gtgaccgcga ggccgtcgat
     1081 tcgcagcgcc cccgaaacca ggtcgaggtc ggcgttccac tgccagatca ggcagccagc
     1141 cgttaccgcg taggtcagca gcgccaggcc cgggccgaag cgcttgaccc ggtcgaagac
     1201 cgcggccagc acgatcacga cgaggccggc ggtcagcgcg atgatcggcg agaggccggc
     1261 gtagtcgatg gtcggtgcgt cgaaggtcac ttcgcgacct
//
LOCUS       DAOTIP010000673         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_124334_length_1298_cov_2.938053, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000673 DAOTIP010000000
VERSION     DAOTIP010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124334_length_1298_cov_2.938053"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="P5138_11055"
     CDS             <1..491
                     /locus_tag="P5138_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV61160.1"
                     /translation="PPNLTDDGLQAAKKIRVDYPDTGVLVLSQYLEESYALDLVADSA
                     EGVGYLLKDRVAEIDRFTESVRRVGSGGSALDPEVVSHLLGRRRREDPLEVLTDRERE
                     VLGLMAEGRSNIAIAESLVVTERAVEKHVTSIFSKLDLPPAAEDHRRVLAVLTYVKAI
                     GD"
     gene            complement(488..>1298)
                     /locus_tag="P5138_11060"
     CDS             complement(488..>1298)
                     /locus_tag="P5138_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRV61161.1"
                     /translation="TSEGRTSAQVSKDANSDSATVAPSSTGVFQLNGTGTNPTSGKRD
                     VARETEITLGTDPDGVQDAANKVIEVTDDHNGIVMKSNVTDGKEGTAGATFSLLIPSG
                     QIEATVADLSGIADLKSRSQDLVDITAPTNRAEDNLADSKAKIKSLLGELEVTYDEDE
                     RAAIEQKIRFERQEKQYYEIRLNRLERRADYTPVDLRVETGGDSASDDGSSSWGLGDA
                     VDDAGKLLGVAAGVTVLALAVAIPIGIVILIALAINRAWVRRSRRRILAED"
BASE COUNT          204 a          440 c          411 g          243 t
ORIGIN      
        1 tgccgccgaa cctcacagac gatggcctgc aggccgcgaa gaagatccgt gtcgactatc
       61 cggacacggg agtgctcgtc ctctcccagt acctggaaga gagctacgcc ctcgacctgg
      121 tcgccgattc ggccgagggt gtcggttacc tgctgaagga ccgggtggcg gagatcgacc
      181 gcttcaccga gtcggtccga cgggtcggaa gcggcggctc cgcgctagac ccggaggtgg
      241 tctcccacct gctcgggcga cggcgacgcg aggatccact cgaggtcctg accgaccgcg
      301 aacgcgaggt cctggggctg atggccgagg gacgttcgaa catcgcgatc gccgagagcc
      361 tcgtggtcac cgaacgggct gtcgaaaagc atgtgacctc gatcttcagc aagctcgacc
      421 tccctcccgc cgccgaggac caccggcggg tgctggcggt cctcacttac gtcaaggcga
      481 tcggcgacta atcctcggcg aggatgcggc ggcgggagcg gcggacccag gcgcggttga
      541 tcgccagggc gatcaggatc acgatcccga tcggaatcgc caccgccagg gccagcacgg
      601 tcacaccggc cgcgacgccg agcagcttgc ccgcgtcatc gaccgcgtcg cccagacccc
      661 agctcgatga cccgtcgtcg gacgcactgt caccaccggt ttcgacccgc agatcgaccg
      721 gggtgtagtc ggcccggcgc tcgagccggt tgagccggat ctcgtagtac tgcttttcct
      781 gcctttcgaa gcggatcttc tgttcgatcg ccgcccgttc gtcctcgtca taggtgactt
      841 cgagctcgcc gagcaggctc ttgatcttcg ccttggagtc ggccaggttg tcttcggccc
      901 gattggtcgg ggccgtgatg tcaaccaggt cctggctgcg ggacttcagg tccgcgatcc
      961 cggaaaggtc cgcaacggtc gcctcgatct gccccgaggg aatgagcaga ctgaaggtcg
     1021 ccccggcagt cccctccttc ccgtcggtca cattcgactt catgactatc ccgttgtggt
     1081 catcggtcac ttcgatcacc ttgttcgccg catcctgaac cccgtcggga tcggtcccga
     1141 gagtgatctc cgtctcccgg gcgacgtccc tcttgccgct agtcgggttc gtgccggtgc
     1201 cattgagttg gaaaactccg gtcgacgaag gcgcgaccgt ggcggaatcc gaattcgcgt
     1261 ccttgctcac ctgcgcactc gtccggcctt cgctcgtt
//
LOCUS       DAOTIP010000674         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_124653_length_1296_cov_2.497985, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000674 DAOTIP010000000
VERSION     DAOTIP010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124653_length_1296_cov_2.497985"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="P5138_11065"
     CDS             <1..907
                     /locus_tag="P5138_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV61162.1"
                     /translation="SAATLMASGGAAALWRRTSNPWGAIGAGPVLASAAGAELADLEF
                     CQFHPTCLSAPGTAFDGALITEAIRGEGAALLDQEGNRFTDELAPRDDVTAAILAQIE
                     RQDGREVRLDLTAIDPARFPNAFELLETAGFDPAREPVPVSPGAHYMMGGISVDLDGR
                     TSLPGLYAAGECACTGLHGANRLASNSLTECFVFGRRAAIAGLGEQPSPAVSLPDWRF
                     DPPQPPTREAVWKFAGPLRNAEGLSHLAGDPYPLARAIGAAAEAREESRGGHRRTDFR
                     ETDPELDFTHLIYRPDGSIEPRRWD"
     gene            913..>1296
                     /locus_tag="P5138_11070"
     CDS             913..>1296
                     /locus_tag="P5138_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRV61163.1"
                     /translation="MAEASGPPHEADRPEGSDPDPIEVQVTAGAIASTCEEMGAVLIR
                     SAHSANIKERRDCSAAIFDPDGEMVMQAEHIPVHLGSMPASVRAVVGKPHVPGVSWFL
                     NDPYSGGTHLPDITVVTPAFAGERLI"
BASE COUNT          214 a          467 c          420 g          195 t
ORIGIN      
        1 cagcgccgcg accctgatgg cgagcggcgg cgccgccgcg ctgtggcgaa ggacctcgaa
       61 tccatggggg gcgatcggag ccggcccggt cctggcctcc gccgcgggcg ccgaactggc
      121 cgatctcgag ttctgccagt tccacccgac ctgcctctca gcccctggca cagccttcga
      181 cggagccctg atcaccgaag cgatcagagg cgaaggagcc gccctgctgg atcaggaagg
      241 caaccggttc accgacgagc tggcaccgcg cgatgacgtg acggcggcga tcctcgccca
      301 gatcgaaagg caggacggtc gggaagtgcg actcgatctc accgcgatcg atccagcccg
      361 tttccccaac gccttcgagt tactggagac cgccggattc gaccccgcgc gcgaaccagt
      421 cccggtttca ccgggcgccc actacatgat gggaggaatc tcggtggacc tcgatgggcg
      481 gacttccctc cccggtctct acgcggccgg tgagtgtgcc tgtaccggac ttcatggggc
      541 aaaccggctc gcctccaact cgctgaccga gtgcttcgtg ttcggacggc gagcagcgat
      601 cgccggcctg ggcgagcaac cctcaccggc cgtttcactc cccgactggc gcttcgaccc
      661 gccgcaaccc cccaccaggg aggcggtctg gaagttcgcc ggcccgctcc ggaacgccga
      721 aggactctcc cacctcgccg gggaccccta tcccctggcc cgcgcgatcg gcgccgccgc
      781 agaggcccgc gaggaatccc gcgggggcca tcgccgcacc gactttcgtg aaaccgaccc
      841 cgaactcgac ttcacccatc tgatctaccg gcccgacggg agcatcgagc cccgccgctg
      901 ggactgaagc cgatggcgga agcctccgga ccgccgcatg aggccgaccg gccggaaggg
      961 tccgatccgg atccgatcga agtccaggtg accgcagggg cgatcgcctc gacctgcgag
     1021 gagatgggag cggtcctgat ccgctccgcc cattcggcga atatcaagga acggcgggac
     1081 tgctcggccg cgatcttcga ccccgacggg gaaatggtga tgcaggccga gcacattccg
     1141 gtccacctcg gttcgatgcc ggcgtcggtc cgtgccgtcg tcgggaagcc ccatgtgccg
     1201 ggggtgtcgt ggttcctgaa tgacccgtat tcgggtggca cccatctgcc cgacataacc
     1261 gtggtgacac ctgctttcgc cggcgagagg ctgatc
//
LOCUS       DAOTIP010000675         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_125169_length_1292_cov_3.608731, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000675 DAOTIP010000000
VERSION     DAOTIP010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125169_length_1292_cov_3.608731"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="P5138_11075"
     CDS             complement(<1..463)
                     /locus_tag="P5138_11075"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRV61164.1"
                     /translation="MIAVAGPGKAAAGTCPANRISTVDGCATRSAVASELTGIIKQNL
                     NQVRLQAVIARVDIAGHRILRRGFGESQEGVPATPAMNFRIGSMTIPALTTLVYQLRQ
                     RGRLKLSDPVSRWLPNLFRASEVTVRNLMNNSSGYYDWIQNNPAFQDEVLAD"
     gene            complement(557..1108)
                     /locus_tag="P5138_11080"
     CDS             complement(557..1108)
                     /locus_tag="P5138_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRV61165.1"
                     /translation="MPRIPYPTEDQLEPEARELMANLPPLNLIRMFANAPAALGPVTD
                     LGQAILLRSELDMRLREIAILAVARACDSDYERLQHENICRAIGMPEEEIRAAADRNP
                     EALDEEARMVWTFADQIARNVRADEDLTAKVLEKLGRRQATELVIACAYYSAMGRIIE
                     TTGVELEETLPTENIDPDEYIDD"
BASE COUNT          205 a          405 c          429 g          253 t
ORIGIN      
        1 gatcggccag cacctcgtcc tggaacgcag gattgttctg aatccagtcg tagtaaccgg
       61 aactgttgtt catcaggttg cggaccgtga cctctgaggc ccggaagagg ttcggcagcc
      121 accgggatac cggatccgaa agcttgagcc gtcccctctg ccgcaactgg tagacgaggg
      181 tcgtcagcgc aggaatcgtc atcgagccga tccggaaatt catggccggg gtcgccggga
      241 ccccttcctg ggattcgccg aagccccgcc tgaggatccg gtgaccggcg atgtcaaccc
      301 tggcgataac agcctgcagt cgaacctggt tcagattctg cttgatgatg ccggtcagtt
      361 ccgaagccac cgcggaccgg gtcgcacaac cgtcgaccgt gctgatccgg ttggcgggac
      421 atgtgccggc ggccgccttg ccgggaccgg cgaccgcgat caccgatgcg aggagaagcg
      481 cccctgccgc gaaatgtttc ctgctcccct gcctgaacaa ccttttcacc gcccctgatc
      541 gcttctgacg agactctcag tcgtcaatgt actcgtcggg gtcaatgttc tccgtgggga
      601 gggtctcctc gagttcgact ccggtcgtct cgatgatccg gcccatcgcc gagtagtagg
      661 cacaggcgat caccagttcc gtggcctgcc ggcggcccag cttttccagc accttcgcag
      721 tgaggtcctc gtcggcccgc acgtttctcg cgatctggtc ggcgaaggtc cagaccatcc
      781 gggcctcttc gtcgagggct tcggggttcc ggtcggccgc ggcgcggatc tcttcctcag
      841 gcatgccgat cgcccggcag atgttctcgt gctggaggcg ttcgtagtcg gagtcacagg
      901 cccgggcgac ggcgagaatc gcgatctcgc ggaggcgcat gtcgagctcg gagcgaagca
      961 ggatcgcctg gccgaggtcg gtgaccggcc ccagggcggc gggtgcgttg gcgaacatgc
     1021 ggatcaggtt gagcggaggc aggttggcca tcagctcgcg agcttcgggt tccagctggt
     1081 cttcggtcgg atagggaatg cggggcatgg tttcctctca ggggtcgggg atcagttcgc
     1141 ggatcgggtc cgagccgtcc cagttatccg cctcggagcg gacatgctcg aagaacatgc
     1201 ccttcggtcc gctcacgtcg gagatctcga tcacggtgcc gggcgtggtc tcggtgtcca
     1261 ggtaggcgaa ccggcccctt tcaccgccga tg
//
LOCUS       DAOTIP010000676         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_125601_length_1289_cov_3.734198, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000676 DAOTIP010000000
VERSION     DAOTIP010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125601_length_1289_cov_3.734198"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5138_11085"
     CDS             complement(<1..255)
                     /locus_tag="P5138_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61166.1"
                     /translation="MVFVDPGQEDRQKRGLPIVPVFDGYRAFAIFGIIIYHLLGVAPF
                     NEPWMDEWVKFQTATFGQLIDVLFMLVLFFVLSASLLQGQI"
     gene            complement(269..>1289)
                     /locus_tag="P5138_11090"
     CDS             complement(269..>1289)
                     /locus_tag="P5138_11090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV61167.1"
                     /translation="VKDGVARLQLAPDGADHVRLTGTKTEIPWADVANEIRESPDYDS
                     WLTTFFDDQLAEIDRACAIAKEDEAWQLFRELQDDLWAVLLTRDYRSYPNIQSVLPDT
                     PELDLQWEWNGAVGLHQLSQSRHFYGRVCELNERHGRVPLNEARILDFGCGWGRLTRY
                     FARHAMPDRLFGCDPTGNILDVCAETGVPGELKKSEFVPESLPFENLDLAFSFSVFTH
                     ISEDAHRACLEAIHNALAPGGLLVVTVRPPSYLELDPPMGPARRGLSDDYVNRELREP
                     RYIFLPHLVEEGHPLHDEGATTYGDTVISFPYIEKNWTDLFEIAETGILTGDMYQVPV
                     ALRKK"
BASE COUNT          239 a          384 c          420 g          246 t
ORIGIN      
        1 aatctgtccc tgcagcaggg aagcggacag gacgaagaaa aggaccagca tgaagaggac
       61 gtcgatcagc tggccaaagg tggcggtctg gaacttcacc cactcgtcca tccagggctc
      121 attgaaggga gctactccga gcaggtggta gatgatgatt ccgaagatcg cgaaggcccg
      181 gtaaccgtcg aagaccggga cgatcggcag cccccgcttc tgccggtcct cctgcccggg
      241 gtcgacgaac accatcaggc cttccggttc acttcttcct gagtgctacc gggacctggt
      301 acatgtctcc ggtgaggatg ccggtttcgg cgatctcgaa caggtcggtc cagttctttt
      361 cgatgtaggg gaagctgatc accgtgtcac cgtaggtcgt cgctccctcg tcatgaagcg
      421 ggtggccttc ttcgacgagg tgggggagga agatgtaacg gggttccctg agttcccggt
      481 tcacgtaatc atcggagagc cctcggcggg ccggccccat cggcgggtcg agctcgaggt
      541 aggagggagg ccgcaccgtg accaccagca ggccaccggg ggcgagggca ttgtggatcg
      601 cctccagaca ggcccggtga gcatcttccg atatgtgcgt gaacacggaa aagctgaaag
      661 ccaggtcgag gttctcgaac ggcaggctct cgggaacgaa ttcggatttc ttcaactccc
      721 ccggtacccc ggtctcggcg cagacgtcga ggatgttgcc ggtcggatcg cagccgaaga
      781 gccggtccgg catggcatgc cgggcgaaat agcgggtcag tcgtccccag ccgcagccga
      841 agtcgaggat ccgggcctcg ttcaacggca cccggccgtg acgttcgttc aactcgcaga
      901 cacggccgta gaagtgtctg ctctggctga gttggtgcag tccgaccgcg ccattccatt
      961 cccattgcag gtcgagttcg ggagtgtcgg gcagcacgga ctggatgttc gggtaactcc
     1021 tgtaatcgcg ggtcaggagc accgcccaga ggtcatcttg cagctcacgg aagagctgcc
     1081 aggcctcatc ttcctttgcg atcgcgcagg ccctgtcgat ctccgccagc tgatcgtcga
     1141 agaaggtcgt gagccacgaa tcgtagtcgg ggctttccct gatctcattg gcaacgtcgg
     1201 cccaggggat ctccgtcttg gtgccggtga ggcggacatg gtccgcgccg tccggcgcaa
     1261 gttgcagcct ggctacgccg tccttcacc
//
LOCUS       DAOTIP010000677         1281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_126849_length_1281_cov_2.096248, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000677 DAOTIP010000000
VERSION     DAOTIP010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126849_length_1281_cov_2.096248"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="P5138_11095"
     CDS             complement(<1..696)
                     /locus_tag="P5138_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61168.1"
                     /translation="MTHARQLIRRIAGNPEEGTAGRKARLAGLIGVPVAGAFLLGVII
                     GVIGQSISAGATFILLAIPVAIAGGLLTGRVVLDGAMQLRGSQKAGWLAEKLEPISVV
                     ADPDQPARVEIIHPAVDLKHFFGGFIAVFNLARRLGEDGHRVRIVTMEPLELPADWRK
                     RVAAYEGIGNSVEDLEVIDGSDRNVPVRMNPEDRLIATHWTAAHVASGVATSLGRDSF
                     TYLIQEYEPLIFPM"
     gene            729..>1281
                     /locus_tag="P5138_11100"
     CDS             729..>1281
                     /locus_tag="P5138_11100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV61169.1"
                     /translation="MDLSVIIPAFNEREALPGLVAEIDSACSGLGLEWETIVVDDGST
                     DGTFDCVAELAEGNHRLGVIRLRRNFGKSAALAAGFDVTRGAKVITIDGDGQDDPADI
                     PRLLEALESDSDLVSGWKSDRQDPFTRRIASKIFNWFTARVTGVKLHDMNCGFKAYRG
                     ECARSIEVYGEMHRFLPAFAAQQG"
BASE COUNT          216 a          421 c          411 g          233 t
ORIGIN      
        1 catcgggaag atcaacggtt cgtattcctg gatcagatag gtgaacgagt cccggccgag
       61 cgatgtggca accccggaag cgacatgggc ggcggtccag tgggtggcga tcagtcgatc
      121 ttccgggttc atccggaccg gcacgtttct gtcgctgcca tcgatcacct cgaggtcctc
      181 gacggagttg ccgatacctt cgtaggcggc aacccgcttt ctccagtcgg ccgggagttc
      241 tagcggctcc atggtcacga tccgcacccg gtgcccgtcc tctcccagcc gtcgggccag
      301 gttgaagacc gcgatgaacc cgccgaagaa atgcttcagg tcgacggccg gatggatgat
      361 ctcgaccctc gccggctggt ccgggtcggc cacgacgctg atcggctcga gtttctcggc
      421 cagccagccg gccttctggc tgccccgcag ctgcatcgca ccgtcgagta caacccggcc
      481 ggtgaggaga ccgccggcga tcgccacggg gatcgccagg aggatgaagg tcgctccggc
      541 cgagatgctc tggccgatta cgccgatgat cacgccgagc agaaaggcac ccgcaaccgg
      601 cacgccgatc agcccggcca gcctcgcctt gcgtccggcg gtgccttctt ccgggttgcc
      661 agcaatccgc cggatcagct gcctggcatg cgtcacgcga gggagcgtat taccttgaac
      721 ggggtcagat ggacctttca gtgatcattc ccgccttcaa cgagcgcgaa gccctgcccg
      781 gcctggtcgc agagatcgat tctgcctgct ccggcctcgg cctcgagtgg gagacgatcg
      841 tggtcgatga cggctccacc gacggcacct tcgactgtgt tgccgaactc gccgaaggca
      901 atcaccggct cggggtgatc aggctccgcc gtaatttcgg caagtcggcc gctctcgctg
      961 ccggttttga cgtgacccgt ggggcgaagg tgatcacgat cgacggtgac ggccaggacg
     1021 atccggccga catcccccga ctgctcgagg cgctcgagtc ggattcggac ctggtttccg
     1081 gatggaagag cgaccggcag gacccgttca cccggcggat tgcctcgaag atcttcaact
     1141 ggttcaccgc ccgggtgacc ggagtgaagc tccacgacat gaactgcggg ttcaaggcct
     1201 accgcggtga atgtgcccgc tcgatcgagg tttatggcga gatgcaccgc ttcctgcccg
     1261 cctttgccgc gcagcagggc t
//
LOCUS       DAOTIP010000678         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_126970_length_1280_cov_2.475102, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000678 DAOTIP010000000
VERSION     DAOTIP010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126970_length_1280_cov_2.475102"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..>1280
                     /locus_tag="P5138_11105"
     CDS             277..>1280
                     /locus_tag="P5138_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRV61170.1"
                     /translation="MTGSATAKLNVMTLKGTPAPGTPAKYNKFKVVKQGPKKAKKILL
                     LEPGTSAGATNFRPLTNSLIKQLKGWQVWSIERRENLLEDHSMLEKYLKDQATNQEML
                     DYYLGWIINSSIQPHFQGKTTEETEYAKKWGLSMVVHDIRKVVKIARKGGRKVVLGGH
                     SLGGSIVTAYATWNFNGKPGAKGLSGLVFIDGAGAREPADLPTAADAQERLDALNEPS
                     ASPFIQLVPPLPWAAGVFNAMGSLAALKDPNSRSLVGDFPLLPADLKPPVSSTNLAQY
                     AYAVDADTGPENLQLVQSHIGDLAPSGDPRGWVNGQLGTAQRAAQVFTEIDGMDGTSW
                     "
BASE COUNT          273 a          398 c          396 g          213 t
ORIGIN      
        1 ggtgcagaac agcgcggcca acgggctggc caagaaggca gtcgctgccg gcaaagtcaa
       61 gaagagcgcg gaaaagacct acaccgtcgc ctagcggagg accgtttcaa agtcagggcc
      121 cgccacggcg ggcccttttt tctggtttga cagactagaa tcgtctggca acgcggctcc
      181 ggggagggcc gcgacgagca agaggagaga tatatgtacg tggttcgcag gggatcgctg
      241 gcactggtcg caattgcggc agctatttgt ctgtgcatga ccggcagcgc gacggccaag
      301 ctgaacgtaa tgaccttgaa gggcactccc gcaccgggaa ccccggccaa gtacaacaag
      361 ttcaaggtcg taaagcaggg accgaaaaag gcgaagaaga tcctcctgct cgaacccggt
      421 acctcggccg gcgctaccaa tttccgcccg ctgaccaatt ccctgatcaa gcagctgaag
      481 ggctggcagg tctggtcgat cgaacgccgc gagaacctgc tcgaagacca ctcgatgctg
      541 gagaagtacc tcaaggatca ggcgaccaac caggagatgc tggactatta cctcgggtgg
      601 atcatcaaca gctcgatcca gcctcatttc cagggcaaga caaccgaaga gaccgaatac
      661 gccaagaagt ggggcctgag catggtggtt catgacatcc gcaaggtcgt gaagatcgcg
      721 aggaagggcg gccgcaaggt ggttctcggc ggccactcac ttggcggatc gatcgtcact
      781 gcctacgcga cctggaactt caacggaaag cccggagcca agggcctctc cggtctcgtg
      841 ttcattgacg gagcgggagc ccgggaacct gcggatcttc cgaccgccgc agacgcccag
      901 gagagactgg atgcgctaaa cgaaccctcg gcgtctccct tcatccagct ggtcccgccg
      961 cttccgtggg cggccggcgt cttcaacgcg atgggatccc ttgcggcgct gaaggatccg
     1021 aactcccgtt cgctggtcgg cgatttcccc ctcctgcccg cggacctgaa acctcccgtg
     1081 tcctctacca accttgccca gtacgcctac gcggtagatg ccgacacggg tcccgagaac
     1141 ctgcagctgg tgcagagtca catcggtgac ctggctcctt ccggcgatcc gcgcggctgg
     1201 gtgaacggcc agctcggcac cgcccagcgg gctgcccagg tcttcaccga gatcgacggc
     1261 atggacggta cgtcctggta
//
LOCUS       DAOTIP010000679         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_127386_length_1277_cov_3.520458, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000679 DAOTIP010000000
VERSION     DAOTIP010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127386_length_1277_cov_3.520458"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="P5138_11110"
     CDS             complement(<1..398)
                     /locus_tag="P5138_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61171.1"
                     /translation="MKFRGNAAKFVALIGAVLTCLVIAAPSTSARSSKPPVFWIDLMG
                     TARQHPGAIFFTANSGSRVLHLKWTGWGKNRTVGRGRYSVSGASIPGHPYPKGPARIV
                     AWKPMRCVPEFGNREGKTILVYRHARLNRPG"
     gene            complement(441..803)
                     /locus_tag="P5138_11115"
     CDS             complement(441..803)
                     /locus_tag="P5138_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61172.1"
                     /translation="MSRIAAFLLVLVLAMAGCGTGDETPRAVINGEIGSAIETAGGET
                     TDWVFDNDCVINGSCTATIAVASFPSRILATNRYVARDSRAFLKGRDVIATAVNRAYS
                     RQCLERFTRPESLIRCLR"
     gene            complement(800..1102)
                     /locus_tag="P5138_11120"
     CDS             complement(800..1102)
                     /locus_tag="P5138_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRV61173.1"
                     /translation="MSDSTPQTHGYIHDKERYLARLKRIEGQARGIHRMVEEEKYCID
                     ILTQISALTSALEGVAIGLLDEHLRHCVVDAAKEGGPEADEKMKEASDAIARLVKS"
     gene            complement(1092..>1277)
                     /locus_tag="P5138_11125"
     CDS             complement(1092..>1277)
                     /locus_tag="P5138_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61174.1"
                     /translation="FAYNIAAIPLAAVGLLNPMLAGAAMAFSSVFVVGNSLRLRNFGR
                     TAEDRTGASAKGPAHVG"
BASE COUNT          215 a          412 c          406 g          244 t
ORIGIN      
        1 cccggccggt tcagcctggc gtgccggtag accaggatgg tcttcccttc ccgattgccg
       61 aactcgggca cgcaacgcat cggcttccag gcgacgatcc tcgccgggcc ctttggatac
      121 gggtggccgg ggatgctcgc tcccgaaacc gagtagcgac cccgcccgac cgtccggttc
      181 ttgccccagc cggtccactt caggtgcagg acccgggaac cggagttggc ggtgaagaag
      241 attgcgcccg gatgctgcct ggcggtgccc atcaggtcga tccagaaaac aggaggcttc
      301 gacgaccggg ccgaagttga cggcgcggca atcacgaggc aggtcagtac cgctccgatg
      361 agcgcgacga actttgctgc gttccccctg aatttcatat tgagctccct gtcggttgtg
      421 ttagccgggc catcatagac ctagcggagg cagcggatca gggattcagg ccgggtgaag
      481 cgctcgagac attgccggga gtaggcccga ttgaccgcgg tggcgatcac atcccggcct
      541 ttcaggaagg ccctgctgtc cctggcgacg tagcgattgg tggcaaggat ccggctcggg
      601 aaagaagcca ccgcaatcgt ggccgtgcag gagccgttga tgacgcaatc gttgtcgaag
      661 acccagtccg tggtctcgcc gccggcggtt tcgatggcag acccgatttc cccgttgatc
      721 accgcccggg gagtctcgtc accggtgcca cagcccgcca ttgccagtac cagcaccagc
      781 aggaaggccg cgatgcggct caagacttga ccagacgggc gatcgcatcc gaggcttcct
      841 tcatcttttc gtcggcctcc ggccctccct ctttcgccgc gtcgaccaca cagtgcctga
      901 gatgttcgtc gagcaggcct atcgccaccc cctcgagcgc cgaggtcagg gcgctgatct
      961 gggtcaggat gtcgatgcag tacttctcct cctcgaccat ccggtgaatt ccccgggcct
     1021 ggccctcgat ccgcttgagc cgggccaggt accgctcctt gtcatggatg taaccgtggg
     1081 tctgtggcgt gctatccgac atgtgctggt cctttcgcgc ttgcgccggt ccggtcctct
     1141 gcagtccggc cgaagtttct gagtctcagg ctgtttccga cgacaaaaac gctcgagaag
     1201 gccatcgcgg ctccggccag catcgggttg agcagaccga ccgcggcaag ggggatcgcg
     1261 gcgatgttgt aggcgaa
//
LOCUS       DAOTIP010000680         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_127987_length_1273_cov_5.259442, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000680 DAOTIP010000000
VERSION     DAOTIP010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127987_length_1273_cov_5.259442"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            683..>1273
                     /locus_tag="P5138_11130"
     CDS             683..>1273
                     /locus_tag="P5138_11130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV61175.1"
                     /translation="MFSRGWLKPDSSLIDIGCGCGRTAAPLRQAAGFFGTYLGIDVDE
                     EMIAWCRTHLTDERFFFEHADTYNSVYNPAGSRDAYRLPVEDASQDLVISYSLFTHLL
                     ERDLLNYVSEASRALKPTGTMVMTVFCLDHPESAAGRLARFSFRHPVGEAMVEDAGLP
                     EAAVAYPSRFLIESCLNSGFGAARVESTPGQSQLIAT"
BASE COUNT          227 a          399 c          384 g          263 t
ORIGIN      
        1 gtcgacgatc tcgtagacca ggtgatgcag cccccggggc ccggtcgagc cgatgtacat
       61 gcccggtcgc ttgcggaccg cctcgaggcc ctcgaggacg gtgatgtcct tggccccgta
      121 ggaggcgtct ccgctgctct tcttctcagc caatcaatac ctttccgaaa actattcggc
      181 ggccattacc ggaccggctg cctgtggaaa aggtcggccc ggacgaaacc gcgagtcagg
      241 tctccataat accatttccg gtcggataaa cccgcttgcc agagccaaaa aatccctgca
      301 aatccgcggt tttgccgatc gatggaggaa atattccgtc gccatggccg caccaatgtc
      361 ccggttcggt cccgagccgg tgcccaactg ctgccggaag cttgctctag ccttccggcg
      421 accgatggaa agtggcggtg aaaggcggaa tatttcgggg cgaattgaac gtctcgtccg
      481 ccggttcatt ccttccaccg cggtgctcac caggggctgg ccggctcgac tgctcgacgc
      541 ggccgacttt cccagtcggc tgtttcaccc ggaatttcgc gaacttcctc ccaatcatct
      601 gcgggtgcgg gtgggaaccg gcaatcgtgt gctgttcaac cagagccagt tccaacggct
      661 gggggctcac gagtgtctcg acatgttttc ccggggatgg ctgaagcccg attcgtcgtt
      721 gatcgacatc ggctgtggtt gcggccggac cgccgcgcct ctgaggcagg ctgccggctt
      781 tttcggcacc tacctcggaa tcgacgtcga cgaggagatg atcgcctggt gcagaactca
      841 cctgaccgat gagcggttct tctttgaaca tgccgacact tacaactccg tgtacaaccc
      901 cgcgggatcg agagacgcct atcgcctccc ggttgaagac gccagccagg atctggtcat
      961 ttcgtactcg cttttcactc acctgctcga gcgggacctt ctgaactatg tctccgaggc
     1021 ctcgcgtgcc ctgaagccca cgggaaccat ggtcatgacc gttttctgtc tcgaccatcc
     1081 cgaatcagcc gcgggaaggc tggcgaggtt cagcttccgg cacccggttg gtgaggcgat
     1141 ggtggaggac gccgggctgc ccgaggccgc cgtcgcgtat ccgtctcggt tcctgatcga
     1201 aagctgtctg aacagcggat tcggagcggc gagagtggag tcaacgccgg ggcagagcca
     1261 gttgatcgcc acg
//
LOCUS       DAOTIP010000681         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_128018_length_1273_cov_3.130542, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000681 DAOTIP010000000
VERSION     DAOTIP010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128018_length_1273_cov_3.130542"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..811)
                     /locus_tag="P5138_11135"
     CDS             complement(299..811)
                     /locus_tag="P5138_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HRV61176.1"
                     /translation="MRLPPVDPTEPPSLYKRAITPIALSSVGQWYLKGPAPVLDDRLF
                     RWTRGRLTSVPGIPILLLKSVGAKSGRPRLNALGYFNDGERVIAMASNYGGTRNPAWY
                     YNVRANPDVTLTAGGREGRYRAEITSGAERDRLWELAKGYIDAYEMYEGMTAGVREVP
                     VIAFTPMDPE"
     gene            complement(891..>1273)
                     /locus_tag="P5138_11140"
     CDS             complement(891..>1273)
                     /locus_tag="P5138_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV61177.1"
                     /translation="AERLLETAIEYANDRQAFGRPIGKFQAIRHKIAAMATKIEASKQ
                     FTYSVAWRVNNGEYPVREISMAKLLSSQMACEVADEVVQIHGGYGYMKEYEVERAWRD
                     LRLNRIGAGTDEVMLEVIGRSYGL"
BASE COUNT          212 a          410 c          416 g          235 t
ORIGIN      
        1 gatcgcgatc gcggtcccgg cgccgccgag cgcgacgatc gtcatggcaa ggccggtcct
       61 gatccggcgg ccggtgcggt gactccagtt gatgcgattt ccaagcctca ttcgacctct
      121 cgctcccatg cggtcaagtt acgacggcga ttctagttct cctgagaaga aaccgtgaac
      181 ttgcagggaa aaaccggtta ccgagtcagg atatggtgat cttcccgtcc tcgacccgcc
      241 cgactgttgt cccggcagcg gcacgttggg ggacactgat cctcgcgccg gtcgacccct
      301 actcgggatc catcggtgtg aacgcgatga cgggaacttc gcggactccg gccgtcatcc
      361 cctcgtacat ctcgtaggcg tcgatgtagc ccttcgccaa ctcccagagc cggtcgcgct
      421 cggctccgct ggtgatctcg gcgcggtacc gaccctcgcg cccgcccgcc gtcagcgtga
      481 cgtcggggtt agcccgaacg ttgtagtacc aggcgggatt cctggtaccg ccgtagttgg
      541 aggccatcgc gatcacgcgc tcaccgtcgt tgaagtagcc cagcgcgttg aggcgtggcc
      601 ggccgctctt cgcgcccacg gacttcaaca gcaggatggg gatgccgggg accgaggtga
      661 gccggccacg ggtccagcgg aagagtcggt catcgagaac aggggccggc cccttcagat
      721 accactgacc gacggaggag agagcgattg gcgtgatagc ccgcttgtac agcgagggtg
      781 gttcggttgg atcgacggga ggcagccgca tcaagtacca agctatcccg gcaatcgcgc
      841 cctggcaagt cgccgaccag ctgtgttcgt cgcttctccg ggcgggaagg ctagaggccg
      901 taggagcggc cgatcacctc gagcatcacc tcgtcggtgc cggcaccgat ccggttcagg
      961 cgcaggtccc gccaggcgcg ctcgacctcg tactccttca tgtagccgta gccgccgtgg
     1021 atctggacca cttcgtctgc cacttcgcag gccatctgcg aggagagcag cttggccatc
     1081 gagatctcgc ggaccgggta ctcaccgttg ttgacccgcc aggcgaccga gtaggtgaac
     1141 tgcttggaag cctcgatctt ggtcgccatg gcggcaatct tgtggcggat cgcctggaac
     1201 ttgccgatcg gacggccgaa ggcctggcgg tcgttggcat attcgatcgc ggtctcgagc
     1261 aggcgctcgg ccg
//
LOCUS       DAOTIP010000682         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_128639_length_1269_cov_2.922570, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000682 DAOTIP010000000
VERSION     DAOTIP010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128639_length_1269_cov_2.922570"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="P5138_11145"
     CDS             <1..190
                     /locus_tag="P5138_11145"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV61178.1"
                     /translation="IFLTKQKTIDQAGPDYASTIELVQEGLTEKVMQELDARVDLDKQ
                     QPADVAHEYLVASGYIAG"
     gene            complement(261..1139)
                     /locus_tag="P5138_11150"
     CDS             complement(261..1139)
                     /locus_tag="P5138_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV61179.1"
                     /translation="MVVSASQETPFGLGDFDYFIQLLSAATGAAVERGRALVVEGVRS
                     GPDAFGTVEVDGAIVVDPVSDDPLIDALDRANVPVVTTGRRDRPPAGGRPECWVDNDH
                     VEATEEILAHLESHGAKKVGLISTAPVTSYTRDAISGYKAWCSLHGQEPMISTVEGPI
                     NEGAGFRAAEELLDLADPPDAIYATLDQLALGVLAAARARSLTVQDDLMVAGCTDSQA
                     SAWADPPLTAVLLNPEEIGRSAVSTLIDLIEAGDSVPDPVVVPTGIVERESTARLTGP
                     GGVQAAGQAGSSRDSE"
BASE COUNT          235 a          407 c          407 g          220 t
ORIGIN      
        1 catcttcctg accaagcaga agacgatcga ccaggccgga ccggattacg cctcgacgat
       61 cgaactggtc caggagggcc tgaccgagaa ggtgatgcag gagctcgacg cccgggtcga
      121 cctcgacaag cagcagccgg ccgatgtggc ccacgagtac ctcgtggcca gcggctacat
      181 cgccggataa ccaggcaaga gtcgatcccg gaagggatcg aggtgaatcg ggccggtcac
      241 cttcgggtgg ccggcctttt tcattcggag tcgcggctgc tgccggcttg accggctgcc
      301 tgcacacctc cggggcctgt cagacgcgcc gtggattcac gctcgacgat gccggtggga
      361 acgacgaccg gatcgggcac agaatccccc gcttcaatca ggtcgatcag agtcgagact
      421 gcggatctgc ctatctcttc aggattgagc aagactgccg tgagcggggg atcggcccag
      481 gcgctggcct gggagtcggt gcatccggca accatgagat cgtcctgcac agtcagggat
      541 ctcgcccggg cggccgccag gactccgaga gccagctggt cgagggtggc gtaaatggca
      601 tcgggcggat cggccaggtc gagaagctct tcggccgcac ggaaaccggc accttcgttg
      661 atcggacctt caaccgtcga gatcatcggc tcctggccgt gcagcgaaca ccaggccttg
      721 tagcccgata tcgcatcccg agtgtatgaa gtgaccggag cagtcgaaat caggcccact
      781 tttttcgctc cgtgcgactc caggtgggcc agtatctcct cggtggcttc gacgtggtcg
      841 ttgtcgaccc agcactcggg acgtccacct gccggcggac gatctcgacg gccggtcgtg
      901 actacgggaa cgttggcccg gtcgagagcg tcgatcaggg ggtcgtccga taccgggtcc
      961 accacaatcg ctccgtcaac ttcgacggtg ccgaaagcgt ccgggccgga tcgaacgccc
     1021 tccaccacca gtgcccgtcc acgctcgacc gcggctcctg tcgcggcgct gagcagctgg
     1081 atgaagtagt cgaaatcacc aaggccaaac ggtgtctcct gactggcgga aacgacgatg
     1141 cccagcagtc cggtcttgcc ggaaaccagg ttccttgcgt tgacgttcgg ccggtagccg
     1201 agctcctcgg caatcgacgc gatcctctgc ctcgtttcga ttgccgagga gctcggctac
     1261 cggccgaac
//
LOCUS       DAOTIP010000683         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_128861_length_1268_cov_2.145919, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000683 DAOTIP010000000
VERSION     DAOTIP010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128861_length_1268_cov_2.145919"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1268
                     /locus_tag="P5138_11155"
     CDS             <1..>1268
                     /locus_tag="P5138_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV61180.1"
                     /translation="EFWLGRQGRITEPVYRPKGSGHYEVIGWEKAFDLIADRLRSLDS
                     PDQAVFYTSGRIMNEPAFLYQLFAREYGTNNLPDCSNMCHEATGAGMGWSIGVGKSTV
                     SYSDFEKADLIMVMGQNPGTNHPRMLNALEGAKRKGARVVGINPLPEAALLRYRNPQK
                     PRGLIGKGTVISDQFVQIRSGGDQHLLRAIGKRVLMAEEAAPGTVIDHEFIEQHCEGF
                     EAYREAALALDDGEVLAATGLSAGEIDELADRYIRSKATIITWALGLTQQKSAVETIT
                     EIMNLLFLKGNIGRPGAGASPIRGHSNVQGDRTMGVWEQMGDWFLDAIRDEFGFEAPR
                     EPGHDTVRTADAMERGEVAFFMSMAGNFVAAVSDSTRAEAGMERVGMTVHVSTKLNRS
                     HVTCGEESLILPVLGRAERDEQASGSQVVT"
BASE COUNT          241 a          401 c          426 g          200 t
ORIGIN      
        1 cgagttctgg cttggccgcc agggccggat caccgaaccc gtctaccgtc cgaagggctc
       61 cggccattac gaggtgatcg gctgggagaa ggcctttgat ctgatcgccg accgccttcg
      121 ttcgctggat tcgcccgatc aggcggtctt ctacaccagc ggccggatca tgaacgaacc
      181 ggcgttcctc taccagctat ttgcccgcga atacgggacc aacaacctgc ccgactgttc
      241 gaacatgtgc cacgaagcga ccggggccgg catgggctgg tcgatcggcg tcggcaagtc
      301 gacggtcagc tattcggact tcgaaaaggc cgacctgatc atggtcatgg gccagaaccc
      361 gggcaccaac catccgcgga tgctcaacgc cctcgagggc gccaagcgga agggcgcccg
      421 ggtcgtcggg atcaatccgc tgccggaggc cgccttgctc cggtaccgga atccgcaaaa
      481 accccgcggg ctgatcggca agggcaccgt gatctccgat cagttcgtgc agatccggag
      541 cggcggggac cagcatctgc tccgggcgat cggcaagcgg gtactgatgg ccgaagaggc
      601 cgcccccggc accgtgatcg atcacgaatt catcgagcag cactgtgaag gctttgaggc
      661 ctaccgcgag gcggcgctcg ccctcgatga cggggaggtg ctcgccgcga ccggtctgag
      721 tgccggggaa attgacgagc tggccgaccg ctacatccgc tcgaaggcga cgatcatcac
      781 ctgggccctg gggctcaccc agcagaagtc cgcggtcgag acgatcaccg agatcatgaa
      841 cctgctcttc ctcaagggca acatcggccg gcccggcgcc ggggcatcgc cgatccgcgg
      901 gcatagcaac gttcagggtg accgcacgat gggggtctgg gaacagatgg gtgactggtt
      961 cctcgacgcg atccgggacg agtttggctt cgaagcgccg cgggagcccg gacacgacac
     1021 cgtgcgcacc gctgacgcca tggaaagggg ggaagtcgcc ttcttcatga gcatggccgg
     1081 aaatttcgtg gccgcggtgt ccgactcaac ccgggccgaa gcagggatgg agcgggtcgg
     1141 aatgacggtc cacgtctcaa ccaagctgaa ccgttcccat gtcacctgcg gcgaggagtc
     1201 gctgattctc ccggtgctcg gcagggcgga acgggacgaa caggcgagcg gcagccaggt
     1261 cgttacgg
//
LOCUS       DAOTIP010000684         1253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_131125_length_1253_cov_2.026711, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000684 DAOTIP010000000
VERSION     DAOTIP010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131125_length_1253_cov_2.026711"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="alaS"
                     /locus_tag="P5138_11160"
     CDS             complement(<1..1189)
                     /gene="alaS"
                     /locus_tag="P5138_11160"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRV61181.1"
                     /translation="MNSAEIRETYLSYFEERGHRRMASASLVPPPEDTSTLLTVAGMQ
                     PFKPYFLGRETPPATRLTSSQTCFRTPDIEEVGKTLRHLTCFEMLGNFSFGDYFKEEA
                     IPFGWQLSTEGFGFDPELIWITVFGGDEELGLGPDEEAIEIWKGLGVPDSRIVKLPRS
                     ENFWQGGPTGPCGPCSEMYLDRGLEFGSEDDRPGDDSDRFLEYWNHVFMSYDQSEDGK
                     LTPLPKKNIDTGMGLERMAAILQGVDSVYDTDLFRPLIGLAEEKSGFRYGEDEAVTRA
                     MRVISDHSRGMTFLLAAGVVPSNEDRGYVLRRVMRRAIQRGRALGLEGSWLGDFTDRT
                     IEMMGGAHPQVVMEKERIDRWVRAEEESFGKTLDRGTALLEEIVDRALEDKNSWISAD
                     DAFK"
BASE COUNT          205 a          401 c          396 g          251 t
ORIGIN      
        1 gcttgaaggc gtcgtcggcg ctgatccacg agttcttgtc ctcgagggcg cggtcgacga
       61 tttcctcgag cagcgccgtg ccgcggtcca gggtctttcc gaagctttct tcctcggccc
      121 ggacccagcg gtcgatccgt tccttctcca tgacaacctg gggatgtgcg ccgcccatca
      181 tctcgatcgt gcggtcggtg aaatcaccga gccagctacc ttcgagtccg agtgccctgc
      241 ctcgctggat ggcccttcgc atcacccgcc tgaggacgta tccgcgatcc tcgttcgagg
      301 ggaccacccc ggcggccagc aggaaggtca tgccacggct gtggtcactg atcacccgca
      361 tcgcccgggt cactgcttca tcctcgccgt atctgaagcc ggacttctcc tcggccaggc
      421 caatcagcgg ccggaagagg tcggtgtcgt aaaccgagtc gacgccctgg aggattgcgg
      481 ccatccgctc taggcccatg ccggtgtcga tgttcttctt cggcaacggg gtgagcttgc
      541 cgtcctcgga ctggtcgtag ctcatgaaga cgtggttcca gtactcgagg aagcggtcgc
      601 tgtcgtcgcc gggacggtca tcttcggagc cgaactcgag tccccggtcc aggtacatct
      661 cggagcaggg cccgcagggg ccggtcggac cgccctgcca gaagttttcc gaacgcggca
      721 gcttcacgat ccgggaatcc ggaaccccga ggcccttcca gatctcgatc gcttcttcgt
      781 ccggtccgag gccgagctct tcatctcccc cgaagacggt gatccagatc agttccggat
      841 cgaagccgaa tccttcggtc gagagctgcc agccgaaggg gatcgcttct tccttgaagt
      901 agtcgccgaa gctgaagttt ccgagcatct cgaaacaggt gaggtggcgc agcgtcttgc
      961 ctacttcttc gatgtcgggc gttcggaagc aggtctggct ggatgtcagc cgggtagccg
     1021 gcggagtctc gcgcccgagg aagtagggct tgaagggctg catcccggcc accgtgagca
     1081 gggtcgaggt gtcctcgggc ggaggcacca gcgaggcgga ggccatgcgc ctgtggcccc
     1141 gctcctcgaa gtaggacaga tatgtctcgc ggatctccgc cgagttcatg ggaaccgcag
     1201 cctactttga tccggtttgg ctggcgatgg tggcatgacc aaccaccacg tca
//
LOCUS       DAOTIP010000685         1250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_131581_length_1250_cov_2.259414, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000685 DAOTIP010000000
VERSION     DAOTIP010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131581_length_1250_cov_2.259414"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1240
                     /locus_tag="P5138_11165"
     CDS             305..1240
                     /locus_tag="P5138_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61182.1"
                     /translation="MKRLAAEASTRFTSLVATGDEIPFDVAENNGDSSHFYRYVPLTS
                     RFITAHIEELKSLPSYGPARGAVASSEVAAPYLESRGLPVPSEPTRRAEEMIAVFITS
                     LWEGTTEFSLDISRVEAALAGLEVQTRDIREADVLIAPLVGFEMTPTEVTLVNGIRIV
                     QADTIEAPIEATSSEGTGRAAWQTAYFALTDLESTEDGPRQAITRLNGLIRALRLYKE
                     GSVGLGPFAFAPVGESSWRRIETTVSPPRAGSYFLIESEVDRLNELITCLAKESPAAD
                     RMEFAQTRFQYGCERENPVDALSDHLLAIRASLGG"
BASE COUNT          237 a          426 c          382 g          205 t
ORIGIN      
        1 cggcgggtgc ggtcgagacg acgccgtttg ttccgaaggt cggatccggg gcgccgggcc
       61 gggccagggc agagccggct acacaggaaa aggcgaccag cagggccggg ataaacacag
      121 ggatacgcaa cgaaaccgac aaggccactc cttggtcatc tggacgacac gcgtgctgct
      181 actccatcag aacgccaaag aaaaagaagt tgctctcaaa tgcaatcaat cctgcaaccg
      241 caggatgcct cgcatgggga gggaactccc aaacaccatg cacgatcctg ttcttgatga
      301 ggctttgaag cggcttgcag ccgaggcgtc gacgcggttc acctccctgg tggccacggg
      361 tgacgagatc ccctttgacg ttgccgagaa caacggggac agctctcact tctaccggta
      421 cgtgcctctc acttcccggt tcatcaccgc ccacatcgaa gagttgaagt cgctgccttc
      481 atacggtccc gcgcgcggcg ccgtcgcctc gtccgaggtt gcggcgccct atctcgagtc
      541 gcgcggactg ccggttcctt ccgagccgac ccgccgggcc gaggagatga tcgccgtctt
      601 catcacttcc ctctgggagg gaaccaccga attttcgctg gacatcagcc gggttgaggc
      661 cgccctcgcc gggctcgagg tccagacccg tgacatccgt gaggccgacg tgctgatcgc
      721 gccgttggtc ggattcgaaa tgaccccgac cgaggtcacc ctcgtcaacg ggatccggat
      781 cgtccaggcc gacacgatcg aagcgccgat cgaagccacc tccagcgagg gaaccggtcg
      841 cgccgcctgg cagaccgcct acttcgccct gaccgatctc gaaagcaccg aagacggccc
      901 ccggcaggcg atcacccgtc tcaacggcct gatccgggcg ctccggctct acaaggaagg
      961 ctcggttggc ctcggcccgt tcgccttcgc gccggtcggc gaatccagct ggcgacggat
     1021 cgagacgact gtctcgccgc cccgcgccgg cagctacttc ctgatcgaat ccgaggtcga
     1081 caggctgaac gagctgatca cctgcctggc gaaggaatcg ccagccgccg atcgcatgga
     1141 attcgcccag acccgcttcc agtacggctg cgagcgcgag aaccccgtgg acgccctgag
     1201 cgaccatctg ctcgcgatcc gcgcttcgct cggaggctaa ggggtgttcg
//
LOCUS       DAOTIP010000686         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_132234_length_1246_cov_1.798489, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000686 DAOTIP010000000
VERSION     DAOTIP010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132234_length_1246_cov_1.798489"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5138_11170"
     CDS             complement(<1..206)
                     /locus_tag="P5138_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61183.1"
                     /translation="MEANEPADEPPATTDPAVFPASPRGIDPPEDPDSYPYATWSPWI
                     AIGGAVSALFLGLLGLAAFMWAMR"
     gene            300..>1246
                     /locus_tag="P5138_11175"
     CDS             300..>1246
                     /locus_tag="P5138_11175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRV61184.1"
                     /translation="MLPATLYGMTRRQRRVRRHRGSPGKVFVAVAAVFGGLLLTAIAA
                     AGGWVLSIAADAPNPDNLKEINKGENSVIYAGDGSKLGLIDSTEVRTPIPLSEIPKSV
                     QEATVAIEDERFYQHDGIDWQGGLRALVKNLESGKIEEGASTITMQLMRNLYISNPER
                     DYKRKIKEAKMALDYETDHSKQEILGKYLNTAPYGTNSGRTAIGISAAAKVYFSLKPE
                     NLTLPQAALLAGLPQAPSDYNPFQNPAGAKQRRNEVLDAMANQNMITEARAAKAKQSG
                     LQLDPAENLFDREDPFFFDYVQQELFKKYGVTPIFGEAT"
BASE COUNT          247 a          419 c          387 g          193 t
ORIGIN      
        1 ctgcgcatcg cccacatgaa cgccgcgagc cccagcagtc cgaggaacag ggcggagacc
       61 gcgccgccga tcgcaatcca gggactccag gtcgcgtagg ggtagctgtc cggatcctcc
      121 ggcgggtcaa ttccgcgggg tgaggccgga aaaacggccg gatcggtcgt ggccggcggc
      181 tcgtctgcgg gttcgttcgc ttccatcgct cgggccattc tcacaattcg gggccgcggt
      241 cgcccgcaaa ccgtattgca gcgactccgg cccggccccg ctctttagaa gttcctgagg
      301 tgcttccagc taccctgtac gggatgacca ggcgccagag aagggtacgc cggcatcgcg
      361 gctcgcccgg caaggtcttt gtcgcagtgg ccgcggtatt cggcggattg ctgctgaccg
      421 cgattgcggc agccggcggc tgggtgcttt cgatcgcggc cgacgcaccc aacccggaca
      481 atctcaagga gatcaacaag ggcgagaact cggttatcta cgcgggcgac ggcagcaagc
      541 tcggtctgat cgattcgacc gaggtccgta ccccgatccc gctttccgag atcccgaagt
      601 cggtgcagga agctacggtc gcgatcgagg acgagcgttt ctaccagcac gacggcattg
      661 actggcaggg cggcctgcga gccctggtca agaacctgga gtccggcaag atcgaagagg
      721 gcgcatcgac gatcacgatg cagctgatgc gcaacctcta catctcgaat ccggagcgcg
      781 actacaagcg gaagatcaag gaagccaaga tggcccttga ttacgagacc gaccactcca
      841 agcaggagat cctcggcaag tacctgaaca cggcccctta cggcaccaac agcggccgga
      901 ccgcgatcgg catcagcgcc gccgccaagg tctatttctc gctgaagccc gagaacctga
      961 ccctgcccca ggccgcgctg ctcgccggcc tgccgcaggc gccttccgac tacaacccct
     1021 tccagaaccc ggccggggcg aagcagcgcc gcaacgaggt cctggacgcg atggcgaacc
     1081 agaacatgat caccgaggcc cgggccgcga aggccaagca gtccggcctt cagcttgatc
     1141 cggccgagaa cctcttcgac cgcgaggacc cgttcttctt cgactacgtc cagcaggaac
     1201 tgttcaagaa gtacggcgtc acccccatct tcggggaggc caccat
//
LOCUS       DAOTIP010000687         1240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_133069_length_1240_cov_3.111392, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000687 DAOTIP010000000
VERSION     DAOTIP010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133069_length_1240_cov_3.111392"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..669
                     /locus_tag="P5138_11180"
     CDS             79..669
                     /locus_tag="P5138_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61185.1"
                     /translation="MLRLVDREIKAWSRGTAQAQLGLLWDEVERNGVKAISWWERDEW
                     SSPYLISASEDRSLAPGKFRAFCQVNFGRPEDDEMLFSDDSEARGRDTPVETMGARDI
                     RRMLELPGVEERPELCDLLINIGYRRDEMDLPAITSFAGHQEPVVRYVVLGMLSWNPS
                     LTNDYLGGKFEKDPDPAVRELAERFASGRLRRQTRI"
     gene            complement(693..>1240)
                     /locus_tag="P5138_11185"
     CDS             complement(693..>1240)
                     /locus_tag="P5138_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV61186.1"
                     /translation="YLGLSEVGPETIRRAAAVHPVADLQIEYSLMSRGIESGILPVTR
                     ELGIGITAYGVLSRGLLSGNWSPGRQFDQTDFRAHAPRFQGDSLRRNLELTERLRAIA
                     DELGATVTQVAIGWVFSRGDDIVPLVGARRRDRLAEAVGAVDLELDQATLDRIGSAIP
                     EDSVAGSRYPEPQMAMLDSED"
BASE COUNT          231 a          390 c          412 g          207 t
ORIGIN      
        1 gtgctggatg gccgacagct cgtcgtggtt gaaccgctcg ggccaggcga acccttaggc
       61 tgagccgcgg gagaaccgat gctgagactg gttgaccggg agatcaaggc ctggagccgc
      121 ggaaccgcgc aggctcaact cgggctgctc tgggatgagg tcgagcgcaa cggggtcaag
      181 gcgatctcct ggtgggaacg ggacgaatgg tcctcgcctt atctgatcag cgcctcggag
      241 gaccggagcc ttgcgccggg gaagttccgg gccttttgcc aggtcaactt cggccggccg
      301 gaagacgacg aaatgctgtt ctccgacgac agcgaggcca gaggtagaga caccccggtc
      361 gagaccatgg gtgcgcggga tatccgccgg atgcttgagt tgcccggcgt cgaagagcgc
      421 cccgaactct gcgacctgct gatcaacatc ggctaccggc gcgacgaaat ggacctcccg
      481 gcgatcacct ccttcgccgg gcaccaggaa ccggtcgtcc ggtacgtggt cctcgggatg
      541 ctttcctgga acccctcgct gaccaacgac tacctcggcg ggaagttcga aaaggacccg
      601 gatccggcgg ttcgggaact cgccgaacgg ttcgccagcg gcagactgcg gcgacagacc
      661 agaatctaga tgcgactgcc ggcataacgg gatcagtctt cgctgtccag cattgccatt
      721 tgaggttccg gatagcggga gccggccaca gaatcttcgg ggattgccga cccgatcctg
      781 tcgagagtcg cctggtccag ttccaggtca actgcgccga cggcctcggc gagccggtcc
      841 cttcggcggg caccgaccag cgggacgatg tcgtcgccgc gggagaagac ccaaccgatt
      901 gcaacctggg tcacggtcgc cccgagttca tcggcgatcg cccggagccg ttctgtcagc
      961 tcgagattgc gccgcagact atcgccttga aaccgcgggg catgagcccg gaaatcagtc
     1021 tggtcgaact gccggccagg gctccaattg ccgctcagaa gtccccggct gagtactcca
     1081 taggcggtga ttccgatgcc aagctcgcgc gtgacgggga gtattcccga ctcgataccc
     1141 cgcgacatca acgagtactc gatctggagg tcagcgacgg gatggacggc cgctgcccgg
     1201 cggatcgttt ccggcccgac ctctgagagg cccaggtagc
//
LOCUS       DAOTIP010000688         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_133887_length_1235_cov_3.522034, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000688 DAOTIP010000000
VERSION     DAOTIP010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133887_length_1235_cov_3.522034"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="P5138_11190"
     CDS             <1..657
                     /locus_tag="P5138_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV61187.1"
                     /translation="AHELRNPLAGISGAIEVLQSGAKDDPPARDHFLNRLSADADRMS
                     RLTQSLLTLARVEALGTGEVEVVDIGSAVKDVEAAVEVPVGLELRTDVEPDLAAKGDP
                     TLLRQVLIGLVTNACKNTPAPGSVTIRGRRAGDSEMLLEVIDTGTGIPQAEIERVFER
                     FYRRSETRRQEGFGLGLAIARRMADVMGGEMGANSVEGEGSTFWVRLPLIDQSETPIA
                     "
     gene            654..>1235
                     /locus_tag="P5138_11195"
     CDS             654..>1235
                     /locus_tag="P5138_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV61188.1"
                     /translation="MNTTSSMNGAAGRILIADDEPSVRDSVGYALAQEGFEVTAAEDG
                     NDAEQRLAGDFDFDLLILDIMMPGKSGLDICREVRSRSSVPIIMLTAKDAEVDKVVGL
                     EVGADDYVTKPFSVRELLGRVRAQLRRRELDRAPATEQTRIAAGPVTIDLARHVVTVN
                     GKTVSLTRSEFQLLRLLAARPGEVFSRAKIMEEL"
BASE COUNT          198 a          427 c          414 g          196 t
ORIGIN      
        1 gcccatgagc ttcgcaaccc gctggccgga atctccggtg cgatcgaggt gctgcagtcg
       61 ggcgccaagg acgacccgcc ggcccgtgac cacttcctca accggctctc cgccgatgcc
      121 gatcggatgt cccgcctgac ccagtcgctt ctcaccctgg cccgggtgga ggcgctcggc
      181 accggcgagg tcgaggtggt tgacatcggc agcgccgtca aggacgtcga ggcggcggtc
      241 gaggtgccgg tcggcctgga actgaggacc gacgtcgagc cggatctggc cgcgaagggc
      301 gacccgaccc tgctgcgcca ggtgctgatc ggcctggtca cgaatgcctg caagaacacc
      361 ccggcccccg gttcggtgac cattcgcggg cgccgggccg gcgattcgga gatgctgctc
      421 gaggtgatcg acaccggcac cggtatcccc caggcggaga tcgaaagggt cttcgaacgc
      481 ttctatcgac gctccgaaac ccgtcgccag gagggcttcg gactgggtct cgcgatcgcc
      541 cgccggatgg ccgacgtgat gggcggggag atgggagcca attccgtcga gggcgagggc
      601 agtacctttt gggtccggct tcctttgata gaccagtcgg agacaccgat cgcatgaaca
      661 cgacttcctc catgaacggc gccgccgggc gcatcctgat cgccgatgac gagccctcgg
      721 ttcgcgattc ggtcggctac gcgctcgccc aggaaggctt cgaggtcacg gccgccgagg
      781 acggcaacga cgccgaacag cgtctcgccg gggacttcga cttcgacctt ctgatcctcg
      841 acatcatgat gcccggcaag tccggtctcg acatctgccg cgaggtccgt tcccgttcct
      901 cggtcccgat catcatgctc accgccaagg acgccgaggt ggacaaggtg gtgggcctcg
      961 aggtcggtgc cgacgactac gtgaccaagc ctttctcggt ccgggagctg ctcggccggg
     1021 tccgggccca gctccgccgg cgggagctcg accgggcccc ggccaccgag cagaccagga
     1081 ttgccgccgg ccccgtcacc atcgacctgg cccgccacgt ggtgaccgtg aacggcaaga
     1141 ccgtcagcct cacccggtca gaattccagc tgcttcggct gctggccgcc cggcccggcg
     1201 aggtgttcag ccgggccaag atcatggaag agctc
//
LOCUS       DAOTIP010000689         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_133892_length_1235_cov_3.361864, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000689 DAOTIP010000000
VERSION     DAOTIP010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133892_length_1235_cov_3.361864"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="P5138_11200"
     CDS             complement(<1..478)
                     /locus_tag="P5138_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61189.1"
                     /translation="MDLTGEKALADLKRCEADLGRYAEERGLKLHSDPAFPGAQRPEE
                     QIGPVRHAVWSLVGEMPGGATARLRHQAIFGEMIGQEVAGHHTVMVTRLPQTVGLVPM
                     LSVRPDEFGAGLFQWTGDGRARQDQTFESIELDRRYLIEVAKGQDQLWLYRLFTPSF"
     gene            555..>1235
                     /locus_tag="P5138_11205"
     CDS             555..>1235
                     /locus_tag="P5138_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61190.1"
                     /translation="MTETATQVLPADDPGPGLSASEAAGRLHDLGPAQPHSSRSTSSI
                     VAGNVFTLFNLIIGIFFVLILSLGLIADAVFGMIAIVNTWIGIRQELKAKETLDRLAI
                     LVAPRAEVIRDGEALELLADEVVPGDLIRMTPGDQLVADGRMVSSRGLTVDESMLTGE
                     SDPIGKSRGDEVLSGGFVISGSGYCEVTAVREESYAGQIAGEAKAFRHPPSPLQEEVN
                     QVILVCTWI"
BASE COUNT          207 a          427 c          383 g          218 t
ORIGIN      
        1 tgaatgacgg agtgaagagc cggtagagcc agagttggtc ctggcccttc gcgacttcga
       61 tcaggtaacg ccggtcgagt tcgatcgact cgaaggtctg gtcctgcctg gcccggccat
      121 cccctgtcca ttgaaagagc ccggccccga actcgtcggg acgaacgctc agcatcggga
      181 ccaggccaac cgtctgcggc agcctggtca ccatcacggt gtgatgcccg gccacctcct
      241 gcccgatcat ctcgccgaat atcgcctggt ggcgcagcct ggcggtggcc ccaccgggca
      301 tctcgccgac cagcgaccag accgcgtgac gaaccggacc gatctgctct tcgggccgtt
      361 gcgcccccgg gaaggccgga tcggagtgaa gcttcagacc gcgctcttcg gcgtatcggc
      421 cgagatcggc ctcgcatcgc ttcaggtcgg ccagtgcctt ttcgccagtc aggtccatcg
      481 ctcccccgct tctggttccg gatatgacga gttttccaca ccggccgacc acccaaaccg
      541 gtaacaatca ccccgtgact gaaacggcca cccaggttct gcccgcggac gatccgggac
      601 ccggcctgtc cgcatccgaa gctgccgggc ggctgcatga cctgggtccg gcccagcctc
      661 actcctcccg ctcgacctca agcatcgtcg cgggcaacgt attcaccctt ttcaacctga
      721 tcatcgggat cttcttcgtg ctgatcctct cgctcggcct gatcgccgac gcggtcttcg
      781 gaatgatcgc gatcgtcaac acctggatcg gcattcgcca ggaactgaag gccaaggaga
      841 cgctcgaccg gctggcgatc ctggtcgcgc cccgggccga ggtgattcgt gacggggaag
      901 ccctcgagct cctggctgac gaggtggttc ccggggacct gatccggatg accccgggag
      961 accagctggt cgcggacggc cgcatggtca gctccagggg cctgaccgtc gacgagtcga
     1021 tgctgaccgg cgagtccgac ccgatcggca agtcccgtgg cgacgaggtc ctctccggcg
     1081 gattcgtgat ttccggttcc ggctactgcg aggtgaccgc ggtccgcgag gaaagctacg
     1141 caggacagat cgcgggcgag gcgaaggcgt tccggcaccc gccgtcgccg cttcaggaag
     1201 aggtcaacca ggtaatcctc gtctgcacct ggatc
//
LOCUS       DAOTIP010000690         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_133942_length_1235_cov_2.405085, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000690 DAOTIP010000000
VERSION     DAOTIP010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133942_length_1235_cov_2.405085"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="P5138_11210"
     CDS             <1..507
                     /locus_tag="P5138_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61191.1"
                     /translation="ERRGNNIPRYRNGKGKIAPYSIANQWCAAFSTWIWNHAGFKAYL
                     GTGILWRSYDGTRVGVQVRDLTNWATKNGYFSYRAKPGYLVAYGTSHIGIVEKIDRIS
                     GRAVGSIEGNIGNRVSRVTVPMDRVTGYISPVRLTPIQLDKSAAYADMAVPSAVLDRL
                     DESGFSQP"
     gene            complement(494..949)
                     /locus_tag="P5138_11215"
     CDS             complement(494..949)
                     /locus_tag="P5138_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61192.1"
                     /translation="MDGVSEDFSGLPEYKSRGERNAERVERRFSQRRNNPYRGHRTRV
                     ILVGGFVVALILLTMSLIVLYVPTPYLGTSHGSLASSIGSSSAQDCRPGGSGWICRKE
                     TGGTVARYDVKVDWAGCWTGELMGSTAASGNAKPEISGCVSIIDHLRAG"
     gene            complement(1051..>1235)
                     /locus_tag="P5138_11220"
     CDS             complement(1051..>1235)
                     /locus_tag="P5138_11220"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HRV61193.1"
                     /translation="GVLVWAPCSGNVSMTISIFSYAGELTVGLMADAGLIEDAGELVA
                     DFETELSSLLELDPEP"
BASE COUNT          241 a          410 c          378 g          206 t
ORIGIN      
        1 gaacggcgcg gcaacaacat cccccgttac cgaaacggca agggcaagat cgccccgtac
       61 agcatcgcca accagtggtg tgccgcattc tcgacctgga tctggaacca cgccggcttc
      121 aaggcctatc tgggaacggg aatcctctgg cgctcatacg acgggaccag ggtgggcgtg
      181 caggtccgcg acctgaccaa ctgggcgact aagaacggtt acttctccta tcgcgccaag
      241 cccggttacc tggtcgccta cggcaccagt cacatcggca tcgtcgagaa gatcgaccgc
      301 atcagcggcc gggccgtcgg ttcgatcgaa ggaaacatcg gcaaccgggt ctcccgggtg
      361 accgtcccga tggaccgggt aaccggttac atcagcccgg tcaggctgac cccgatccag
      421 ctcgacaaga gcgccgcata tgccgacatg gcggttccgt cggcggtact ggaccggctc
      481 gacgaatcgg gtttcagcca gccctgaggt gatcgatgat cgaaacgcaa ccggagatct
      541 ccggcttcgc gtttccgctt gcggcggttg agcccatcag ctctcccgtc cagcatccgg
      601 cccagtcgac cttgacgtcg taacgggcaa cggtcccgcc ggtctccttg cggcagatcc
      661 atccgctgcc gccgggccgg caatcctgcg ccgaggagct gccgatcgag ctggccagcg
      721 agccgtggct ggtaccgaga tacggggtag ggacgtagag cacgatcagc gacatggtca
      781 gcaggatcag ggcgaccacg aagccgccga ccaggatcac ccgggtccgg tggccgcggt
      841 aggggttgtt gcggcgctga ctgaaccggc gttccacccg ttcggcgttt cgctcccccc
      901 gggacttgta ctccggcaat ccgctgaaat cttcgcttac tccatccatc gagcgagcag
      961 ggtagatccc tcctcggcaa agctcacggc attcctgccg ctgaaaggga aatcccttgc
     1021 aaacctgacc gggcccgggg ctgcccggga ctacggctcc ggatccagct cgagcaaact
     1081 ggaaagctcg gtttcgaagt cggcgacgag ttccccggca tcctcgatca gcccggcgtc
     1141 tgccatcaac cccacggtca attcaccggc gtagctgaat atggagatcg tcatcgagac
     1201 gttgccggag caaggtgccc agacaagaac gccgg
//
LOCUS       DAOTIP010000691         1228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_135131_length_1228_cov_2.483376, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000691 DAOTIP010000000
VERSION     DAOTIP010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135131_length_1228_cov_2.483376"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="P5138_11225"
     CDS             <1..319
                     /locus_tag="P5138_11225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV61194.1"
                     /translation="DWEERTRIEDHIARYGGEEFVITLPNTDLEGARVIVERLRTDLP
                     LPSEDRSSGITCSAGIAEWDGAETVRELVARADQALYQAKKEGRNRTVAVESSAERAG
                     FSA"
     gene            complement(338..772)
                     /locus_tag="P5138_11230"
     CDS             complement(338..772)
                     /locus_tag="P5138_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61195.1"
                     /translation="MSEEPQNDPSPNEPPPLTDEERAMLEAQMKEIRVEDLLTQTVAS
                     VLNLSARRIVKEDEVDLDQARLGIESVRSIVALLPPEVGQAIAEPLSQVQLLYAQKSG
                     ETGTGGEEGGPGPGAGPGGGDEPQGPPPAGQGPSKLWTPGGD"
     gene            complement(806..>1228)
                     /locus_tag="P5138_11235"
     CDS             complement(806..>1228)
                     /locus_tag="P5138_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRV61196.1"
                     /translation="HHAFGTVEVRICDAQPTGPESTALAGLMMAVVAQTAVDLDEGRI
                     SGDPLGDRQIEENLWRAIRFGMDGKMIDFDRREEVEARQVLEEMIEWTGPVRSELGID
                     IELPELNGAQRMRSDLESGASIEEIYRQSVADAAATFR"
BASE COUNT          191 a          423 c          390 g          224 t
ORIGIN      
        1 cgactgggag gagaggaccc ggatcgagga tcacatcgcg aggtacgggg gtgaggaatt
       61 cgtcatcacc ctccccaaca ccgacctcga gggcgccagg gtgatcgtcg agcggctccg
      121 cacagacctg cccctcccct ccgaagaccg gagttcgggc atcacctgtt ccgccggcat
      181 cgccgaatgg gacggtgccg agaccgtgag ggaactcgtc gcccgggcag accaggcgct
      241 ctatcaggcc aagaaggaag ggcgcaaccg gacggttgcc gtggaatcca gcgccgagag
      301 ggccggcttt tctgcctgag ccgttccccg cggtcgatca gtcgccgccg ggagtccaga
      361 gcttcgaggg accctgcccg gccgggggtg gcccctgggg ctcgtctccg ccaccgggcc
      421 cggcaccggg tccggggccg ccttcttctc caccggtccc ggtttcgccg ctcttctgtg
      481 cgtaaagaag ttgaacctgg ctgagaggct cggcgatcgc ctggccgacc tcgggtggga
      541 gcagcgccac gatcgaacgc acggattcga tgccgagtct ggcctggtcg aggtcgacct
      601 cgtcttcctt gacgatcctg cgcgccgaga ggttgaggac gcttgcaacc gtctgggtca
      661 gcaggtcctc gacccggatt tccttcatct gggcttccag catcgcccgt tcttcatcgg
      721 tcaggggcgg cggctcgttc ggagagggat cgttctgcgg ttcttcgctc atgactccac
      781 tctagagcca ctgcccaagg ggcacctacc tgaaggtggc ggccgcgtcg gcaacggact
      841 ggcggtagat ctcctcgatc gacgcgcccg actccagatc ggacctcatg cgctgggccc
      901 cgttcagttc cggcagctcg atatctatgc cgagctcgct cctgaccggt ccggtccact
      961 cgatcatctc ttccagtacc tgccgggcct cgacttcctc gcggcggtcg aagtcgatca
     1021 tcttgccgtc catgccgaaa cggatggccc gccacaggtt ctcctcgatc tgtctgtcac
     1081 cgagcggatc gccgctgatt cgaccttcat ccaggtcgac cgcggtctgg gcgaccaccg
     1141 ccatcatcag cccggccaag gcggttgact ccggaccggt cggctgggca tcacagatcc
     1201 gcacctcgac cgtcccgaag gcgtgatg
//
LOCUS       DAOTIP010000692         1225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_135592_length_1225_cov_2.845299, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000692 DAOTIP010000000
VERSION     DAOTIP010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135592_length_1225_cov_2.845299"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1079
                     /locus_tag="P5138_11240"
     CDS             144..1079
                     /locus_tag="P5138_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRV61197.1"
                     /translation="MLDSNHNVVSYRRRPTPDKGGPADVIRAMAVTMQEAAMDAGTEA
                     HRLLGVGVGSPGSVNSETGAVSGAGNLPDWDGTFQMRMALREELGTEIRVGNDVQVGT
                     RGEFELGAGVGYTSLLGVFWGTGVGGGIIINSEPWIGRGRAGEIGHTLVRRGGAKGLN
                     GLNGTVEAYAGRKAMEAKARREVKKGRKTKLFKLMKKHEKTNLSSAIWQRALEDGDEL
                     AEDLLNRAVGALSAGIASAVNLLDPEAVVIGGGLGVRFADTLVPEIIAGMDQYLLDKD
                     NPPDVKVAALGDEGGVIGASLLVKDLLQANQAPSH"
BASE COUNT          227 a          384 c          447 g          167 t
ORIGIN      
        1 aaacccgggc cgaagaggaa aagcggcccg aagtcgacct gccgccgatc acaccgccag
       61 gcgtgttgcc cgggcgcgcc gcgcgtcgca ccgccgcact catcggcggc atcgacctgg
      121 gcggaaccaa gatcgccgcc gccgtgctcg actcaaacca caacgtggtc tcctaccggc
      181 gtcggccgac accagacaag ggtggcccgg cggatgtcat ccgggcgatg gccgtgacca
      241 tgcaggaagc tgcgatggat gccgggaccg aagcccatcg actgctggga gtcggggtcg
      301 gctccccggg ttcggtgaac agcgaaaccg gtgcggtgag cggcgccggg aatctgcccg
      361 actgggatgg caccttccag atgcggatgg cgctgcgcga ggaactcggc accgagatcc
      421 gggtcggcaa cgacgtgcag gtcgggaccc gcggtgagtt cgaactcggc gcgggggtcg
      481 gttacacaag cctgctcggg gtcttctggg gcaccggcgt cggtggcgga atcatcatca
      541 acagcgagcc atggatcggc cggggccggg ccggggagat cggccacacc cttgtaagga
      601 ggggcggagc gaagggtctc aacggactca acggaacggt ggaggcctat gcgggccgca
      661 aagccatgga agcgaaggca cgccgcgagg tgaagaaggg caggaagaca aagctcttca
      721 agctgatgaa gaagcacgaa aaaacgaacc tctcctcggc gatctggcag cgtgccctcg
      781 aagacgggga cgaactggcc gaggacctgc tcaaccgggc ggtcggtgcg ctctccgccg
      841 gaatcgcctc ggcggtcaac ctgctagatc cggaagccgt ggtgatcggt ggcgggctcg
      901 gcgttcgttt cgccgacacg ctggttcccg agatcatcgc ggggatggat cagtacctgc
      961 tcgacaagga caatccgccc gacgtgaagg ttgccgccct gggcgacgag ggcggcgtga
     1021 tcggcgcctc gctgctggtc aaggacctgc tgcaggctaa tcaggcccct tcgcactgac
     1081 cggcccggcg cgaggcgcag gggctccggc gcgaggtcca ggggcccggg gcgaacctca
     1141 ggggctgagc gcgagtttgt ggccgggact tcgggactgg cgctcccagg cctcggccac
     1201 gtctgccaga gccaggactt caacc
//
LOCUS       DAOTIP010000693         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_135733_length_1224_cov_3.575706, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000693 DAOTIP010000000
VERSION     DAOTIP010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135733_length_1224_cov_3.575706"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="fdhD"
                     /locus_tag="P5138_11245"
     CDS             <1..891
                     /gene="fdhD"
                     /locus_tag="P5138_11245"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRV61198.1"
                     /translation="MGRVVSERMVTRFRSGSRPSRRRDRLAGEEPLEIRVNGTPLAVT
                     MRTPGSDYELAAGFLVSEGVISKAADFGGATYCRGGSGPGAANEYNVLDVLLGPGVEP
                     PSPELTRNFYTTSSCGICGKSGIGEIGVDSAFDITGFEGEVELELLLSLPDRLRGSQE
                     VFDETGGLHAAAIFDAASGEMLVSREDVGRHNAVDKVIGWALMEGKLPLTGCVLQVSG
                     RAGFELVQKAKMAGLPVFSAVSAPSTLAVELASASGITLAGFVRGDSLVAYSRPDRIR
                     SVGETPGTAGPDQLPRAVAP"
     gene            888..>1224
                     /locus_tag="P5138_11250"
     CDS             888..>1224
                     /locus_tag="P5138_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61199.1"
                     /translation="MSERPPEQDFNDDGIKVGPPKKVAAGLPSVAFAMGPMLRNAGPR
                     KTLRSSFTMNHKRGFDCPSCAWISEDRPPAIDFCENGMKSLNSEIAPVTVPREFWAGN
                     SLAGMRDKSE"
BASE COUNT          200 a          391 c          421 g          212 t
ORIGIN      
        1 atgggccgag tcgtttcaga gcgcatggtc acccggtttc gctccggttc aaggccaagt
       61 cgccgccggg acagactggc cggcgaggag ccgcttgaga tccgggtcaa cggcacgccg
      121 ctggcggtga ccatgaggac gcccggcagc gactacgaac tggcggccgg ctttctcgtt
      181 tcggaagggg tgatctcgaa ggcggccgat ttcggtggcg ccacctattg ccgtggcggt
      241 tcgggtccgg gggcggcgaa cgaatacaac gtgctcgacg tgctgctcgg tcccggggtg
      301 gagccacctt ctcccgagct gaccaggaac ttctacacga cgagttcctg cggcatctgc
      361 ggcaagtccg ggattggcga gatcggagtc gattcggcct tcgatatcac cgggttcgag
      421 ggcgaagtcg agcttgaact cctgctgagc ctgccggacc ggctgcgggg aagccaggag
      481 gtcttcgatg agacgggcgg ccttcacgct gctgccatct ttgacgcagc cagcggcgaa
      541 atgctggttt cccgggagga tgttggccgt cacaacgcgg tcgacaaggt gatcggctgg
      601 gcgctgatgg aagggaagtt gccactgacc ggctgcgtgc tccaggtctc cggccgggcc
      661 ggcttcgaac tggtccaaaa ggcgaagatg gccggtttgc cggtcttctc ggccgtttcc
      721 gcaccgtcaa ccctcgcggt ggagctggcc tcggcttcgg ggataaccct ggccggcttc
      781 gtccggggcg attcactggt cgcctacagc cggcccgacc gaatccgctc ggttggagaa
      841 accccgggca ccgccgggcc ggaccagctt ccccgggcgg tcgcgccttg agcgaacggc
      901 cgccggagca ggacttcaac gacgacggga tcaaggtcgg cccgcccaag aaggtcgccg
      961 ccgggttgcc ctccgtcgct ttcgcgatgg gcccgatgct gaggaacgcc gggccgcgca
     1021 agacccttcg ttcctccttc accatgaacc acaagcgcgg cttcgattgc cccagctgcg
     1081 cctggatcag cgaggacagg ccaccggcga tcgacttctg cgaaaacggc atgaagtccc
     1141 tcaattccga gatcgccccg gtgaccgtgc cccgggaatt ctgggccggg aactcgctgg
     1201 ccgggatgcg cgacaagtcc gagt
//
LOCUS       DAOTIP010000694         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_136086_length_1222_cov_2.526992, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000694 DAOTIP010000000
VERSION     DAOTIP010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136086_length_1222_cov_2.526992"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /locus_tag="P5138_11255"
     CDS             <1..>1222
                     /locus_tag="P5138_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61200.1"
                     /translation="YLVGLNTNLRISTRPVFAIDLTGYGSLAGLPISETKIHGDADGY
                     FSYSSKGEIDLGIVSASNEMGGFIDAARGLFAASVAYDGCVGKSPFKICSGFDGLVST
                     KGIAGCAYGRIGFGYRWGKTPELLGPPSCDTGDFEVRSAPLVEGGPRAGDRSITIPKG
                     LRGATLRLTGDTGSPSVIMISPAGERITPLAPDPAGQQPVTALTGDNQTLIGLWRPAS
                     GAWTIENQPGPVITGVEFARSLAPPTASGRIKRGKGRSRTLVFKATSRSGLRTTFFER
                     VGVGISRIGSTTKAHGKIRFSAADGPAGKRAVFALIEQDGLPRVRRKITSYRAPGPIR
                     PRVRGLKARRKRSTVSFRWRKASGAREYLVRVRVSDGRNLQFLIRKTHLRVGRVPKGD
                     RIRITVLARSKAGRS"
BASE COUNT          219 a          402 c          403 g          198 t
ORIGIN      
        1 tatctggtgg gcctgaacac caacctcagg atctcgacca ggccggtatt cgcgatcgac
       61 ctgaccggct acgggagcct cgccgggctg ccgatctccg agacgaaaat tcacggtgat
      121 gccgacggtt acttctccta cagctcgaag ggcgagatcg acctcggaat cgtctcggcc
      181 agcaacgaga tgggcgggtt catcgacgcc gcccggggac tcttcgctgc ttccgtcgcc
      241 tacgacggtt gcgtcgggaa gtcgcccttc aagatctgtt ccggcttcga cggtctcgtg
      301 tcgaccaagg gcatcgccgg ctgcgcctac ggccgaatcg gattcggcta ccggtggggc
      361 aagacgccgg aattgctggg tcctcccagc tgcgacaccg gcgactttga agtccgctcc
      421 gcaccgcttg tcgaaggcgg acccagggcc ggtgaccggt cgatcaccat cccgaagggg
      481 ctcaggggtg ccacgctcag gctcaccgga gataccggct cgccatcggt gatcatgatc
      541 tcgccggctg gcgaaaggat cactccgctc gccccggacc ctgcaggtca gcagccggtc
      601 accgcactca ccggcgacaa ccagaccctg atcgggttgt ggcgacccgc ttccggggcg
      661 tggacgatcg agaaccagcc gggcccggtc atcaccggtg tcgagtttgc gcgcagcctc
      721 gccccgccga ctgccagcgg caggatcaaa cgaggcaagg gcaggtccag gaccctcgtc
      781 ttcaaggcga cttcgcgatc cgggctcagg accaccttct tcgagagggt cggggtcggc
      841 atcagccgga tcgggtcaac cacgaaagca cacgggaaga tccgcttcag cgcagccgat
      901 ggtcccgccg gcaagcgggc cgttttcgcc ctgatcgagc aggacggcct gcccagggtg
      961 cgcaggaaga tcaccagcta ccgggccccg ggaccgattc gtccccgggt caggggactg
     1021 aaggccaggc gcaagcgatc gacggtcagc ttcaggtgga gaaaagcctc gggcgcccgt
     1081 gagtacctcg tccgggtgcg ggtatccgat ggtcgcaatc tgcagttcct gatccgcaag
     1141 acgcatcttc gcgtcggcag ggtcccgaag ggcgaccgga ttcggatcac ggtgctggcg
     1201 cgatcaaagg ccgggagatc gg
//
LOCUS       DAOTIP010000695         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_136236_length_1221_cov_2.899657, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000695 DAOTIP010000000
VERSION     DAOTIP010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136236_length_1221_cov_2.899657"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="P5138_11260"
     CDS             <1..559
                     /locus_tag="P5138_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRV61201.1"
                     /translation="AMGGRITIDSATLMNKGFEMIEAHHLFGTGYDRIDVVVHPQSIV
                     HSLIDLCDGATLAHLGYPDMRVPIAYALTWPERAEVPVERLDLAKVGSLDFEEPDPDT
                     FRCLALAREAGQSGGIAPCVLNAADEVAVAAFLDGRISFSDIPRVIESTLDDVGSGPA
                     RGFDELFAVDEASRERASEIISGRS"
     gene            556..>1221
                     /locus_tag="P5138_11265"
     CDS             556..>1221
                     /locus_tag="P5138_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV61202.1"
                     /translation="MSFLVAFLAFSALIVLHEAGHFVAAKATGMRVERFFLFFPPKLV
                     SIKRGETEYGIGAIPLGGFVRITGMNPDEELPPEVAARGYYHQPVWKRIVVIGAGPAV
                     NIAIAFLILWVLAFGIQQPSQNVEQIQPGSPAAAKLQAGDRIISVDGVSGSQEAIADQ
                     IASHRCPGRQTDGCKAGKPATVVVRRDGQKETLRITPVYDASVDRSRLGFAFGLEDAN
                     PGPV"
BASE COUNT          207 a          395 c          402 g          217 t
ORIGIN      
        1 ggcgatgggg ggaaggatca cgatcgactc cgcgacgctg atgaacaagg gcttcgagat
       61 gatcgaggcg catcaccttt tcgggaccgg ttatgaccgg atcgacgttg tggtccatcc
      121 gcagtcgata gtccactcgc tgatcgacct ctgcgacggt gccaccctcg ctcatctcgg
      181 ctatccggac atgcgggttc cgatcgccta cgcgctcacc tggcccgaga gggctgaggt
      241 accggtcgag cggctggacc tggcgaaggt gggcagcctc gatttcgaag agccggaccc
      301 ggacactttc cgctgcctcg cgctggcccg tgaggccggt cagtccggag gcatcgcccc
      361 ctgcgtgttg aacgccgcgg acgaggtggc cgtggccgcg tttcttgacg gacggatctc
      421 cttcagcgac attcccaggg tgatcgagag cacccttgat gacgtcggaa gtggccctgc
      481 aagagggttt gacgagcttt tcgcggtcga cgaggcaagc cgggagcgcg cttctgagat
      541 catttctggt cgctcttgag cttcctcgtt gcattcctcg cattcagcgc cctgatcgtc
      601 ctccacgagg ccgggcactt cgtcgctgca aaggcgaccg gaatgagggt ggagaggttc
      661 ttcctcttct tcccgccgaa gctggtctcg atcaagcgcg gcgagactga atacggcatc
      721 ggtgctatcc cgctcggcgg attcgtgcgg atcaccggca tgaacccgga cgaggaactg
      781 ccgccggaag tcgccgcccg cggctactac caccagccag tctggaagcg gatcgtggtg
      841 atcggggccg gtccggcggt caacattgcg atcgcgttcc tgatcctctg ggtgcttgcc
      901 ttcgggattc agcagccaag ccagaacgtc gaacagatcc agcccggcag cccggcagcc
      961 gcgaagctcc aggccggcga ccggatcatc tcggtggacg gggtcagcgg cagccaggag
     1021 gcgatcgccg accagatcgc gtcccaccgc tgtccgggca ggcagaccga cggctgcaag
     1081 gccggcaagc cggcgaccgt cgtcgtcagg cgcgacggcc agaaggaaac gcttcgcatc
     1141 accccggtct acgacgcctc ggttgaccgc agccggctcg gcttcgcatt cggtctcgag
     1201 gacgcgaacc ccggaccggt c
//
LOCUS       DAOTIP010000696         1218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_136704_length_1218_cov_3.883921, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000696 DAOTIP010000000
VERSION     DAOTIP010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136704_length_1218_cov_3.883921"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>1218
                     /locus_tag="P5138_11270"
     CDS             100..>1218
                     /locus_tag="P5138_11270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV61203.1"
                     /translation="MSPFSESSVGEESARPHPMRSLMILGLAALSFALAQTTLIPALA
                     ELMEEFHTDSTGIAWVLTGYLISAAIFTPIFGRLGDMFGKRRLLVIALFIFAAGSTVS
                     ALGDSVPAIVAGRILQGVGGGIFPLCFGIIRDEFPPDRVSASVGLMSATLGIGGGIGL
                     VVGGLIVDHASYHWIFWLGAAMGVIAAIAALLFVPESPNRTPARVDYRGALVLAVGLS
                     VPMYAISEANNWGWASGKTIGLILAGLAVIAFWVWLQRRTDEPLADVSLLADRTVAMT
                     NFATLLIGFGMFASFILIPQLVEAPEATGYGFGFSATQAGLVMLPAALMMLFAGPLSG
                     RIGAKYGNKLPLALGGLISGAGLVGMGLDHGSALSIVLW"
BASE COUNT          167 a          423 c          382 g          246 t
ORIGIN      
        1 gaactcgacc cggacaggac cttcagcaac gactacgtga agagggtgct cggggattag
       61 cggaccctcg ggtccggtta gttgttacta tccggaccag tgagtccgtt tagtgaatcg
      121 tccgtcgggg aagagtcggc ccgtccccac ccgatgcgca gcctgatgat cctgggtctt
      181 gccgcgctct cgtttgccct cgcgcagacc accctgatcc ctgccctggc cgaactcatg
      241 gaggagttcc acactgactc gaccggcatc gcctgggtcc tgaccggcta cctgatctcg
      301 gccgccatct tcacaccgat cttcgggcga ctcggagaca tgttcggcaa acgccggttg
      361 ctcgtcatcg ccctcttcat cttcgccgcc ggctccacgg tctcggccct cggagacagc
      421 gttcccgcca tcgtcgcggg ccgcatcctc cagggcgtcg gcggcggaat cttccccctc
      481 tgtttcggga ttatccggga cgagttcccg cccgaccggg tctccgcttc ggtcgggttg
      541 atgtccgcca cgctcgggat cggcggcggc atcggcctgg ttgtcggcgg actgatcgtc
      601 gatcacgcct cctaccactg gatcttctgg ctgggtgccg caatgggcgt gatcgccgcg
      661 atcgcggcgc tgctcttcgt gcccgaatcg ccaaaccgga cgccggccag ggtggactac
      721 cgcggggccc tcgttctcgc agtcggcctt tccgtcccga tgtacgcgat ttcggaggca
      781 aacaactggg gatgggccag cggcaagacg atcggcctga ttctcgccgg cctggccgtg
      841 atcgccttct gggtctggct tcagaggcga accgacgagc cgctggccga cgtctccctg
      901 ctggccgacc ggaccgtggc gatgaccaac ttcgcgaccc tgctgatcgg cttcggcatg
      961 ttcgcctcct tcatccttat cccgcaactg gtcgaggctc cggaagcgac cggttacggc
     1021 ttcgggttca gcgcgaccca ggccgggctg gtcatgctcc ccgccgccct gatgatgctc
     1081 ttcgccggtc cgctctccgg gcggatcggc gcgaaatacg gcaacaagtt gcctctcgcg
     1141 ctgggcgggc tgatcagcgg tgccggactg gtcggcatgg gcctggacca cggctcggcg
     1201 ctctccatcg tgctctgg
//
LOCUS       DAOTIP010000697         1213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_137724_length_1213_cov_1.724525, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000697 DAOTIP010000000
VERSION     DAOTIP010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137724_length_1213_cov_1.724525"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="P5138_11275"
     CDS             <1..243
                     /locus_tag="P5138_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61204.1"
                     /translation="DRPEERVKRGFVRTRLLEDLSRTVSEAGVNIISANLTTNPPMVK
                     NRFVVEVGDTGQLKQCINRIRHVESVFDAHRVNATD"
     gene            248..>1213
                     /locus_tag="P5138_11280"
     CDS             248..>1213
                     /locus_tag="P5138_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61205.1"
                     /translation="MGRTIAFFPEGAYGPTNNCVGIGNVLRERGHRVVFIIEESFAGT
                     LEAQGFEERLMRLGPPPEEEEAPGQFWKDFVAENAPVFQTPTIEQLTGFIEPTFRALY
                     DGAVYVDDRLTEIIEEIDADAIVEDNVIAFPALPASGRPWVRIVSCNPTEMVDPDLPP
                     VFSGLPQADRSGWPEFWAEYDRVLTPLHGEFSEFCQSRGASPLPARAFIHESPHLNFY
                     AYPSEADYRRSAGLGPLWHNLDSTIRSGDEDWASPPEIAEGEGSLVYLSLGSLGSADT
                     ALMQGLIDTLDASPHRVIVSMGPLHDQLELGERMVGAEFLPQPSVL"
BASE COUNT          206 a          408 c          387 g          212 t
ORIGIN      
        1 gaccggccgg aggagcgcgt gaagcgaggc ttcgtccgca cccgcctgct cgaggatctt
       61 tcccgcaccg tctcggaggc cggggtcaac atcatttcgg ccaacctcac caccaacccg
      121 ccgatggtga aaaaccgttt cgtggtcgaa gtcggagaca ccggccagct caagcaatgc
      181 atcaaccgca ttcgccatgt cgagagcgtc ttcgatgctc accgggtgaa cgcgacggac
      241 tgatcagatg ggacggacga tcgccttttt ccccgagggt gcctacggtc cgaccaacaa
      301 ctgcgtggga atcggcaatg tcctgcgtga gcggggacac cgcgtcgtct tcataatcga
      361 agagtccttc gccggcacgc tggaagcgca gggattcgag gagcggctga tgcgactggg
      421 gccgccaccg gaggaagagg aggcgcccgg tcagttctgg aaggacttcg tggccgagaa
      481 tgccccggtc ttccagacac ccaccatcga gcagctgacc ggcttcatcg agccgacctt
      541 ccgggccctc tacgacgggg ccgtttacgt ggatgaccgg ctgaccgaga tcatcgagga
      601 gatcgacgcc gacgccatcg tggaggacaa cgtgatcgcc tttccggcct tgccggcgag
      661 cggccggccg tgggtgcgga tcgtgtcctg caacccgacc gagatggttg acccggacct
      721 gccaccggtc ttctccggcc tgccgcaggc ggaccgatct ggctggccgg agttctgggc
      781 cgagtacgac cgggtgctga ccccgctcca cggtgagttc agcgagttct gccagtcccg
      841 gggcgcgtca ccgcttccgg cacgcgcatt catccacgag tcgccccatc tgaacttcta
      901 cgcctacccg tccgaagctg actaccggcg ctcggccggc ctcggcccgt tgtggcacaa
      961 cctggactcg acgatccggt ccggcgatga agattgggcc tcgcctcccg aaatcgccga
     1021 gggtgaaggg tcactcgttt acctcagcct gggttccctc gggtccgccg acacggcgct
     1081 catgcagggg ctgatcgaca ctctcgacgc ctccccgcac agggtgatcg tctcgatggg
     1141 tccgctgcac gaccagctgg agctcggtga gcgcatggtc ggggccgagt tcctgcccca
     1201 gccctcggtg ctt
//
LOCUS       DAOTIP010000698         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_138128_length_1210_cov_3.877922, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000698 DAOTIP010000000
VERSION     DAOTIP010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138128_length_1210_cov_3.877922"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="P5138_11285"
     CDS             complement(<1..856)
                     /locus_tag="P5138_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV61206.1"
                     /translation="MATDLTRTILAVDDDTEALGVIESALRGRYGSEYEVICSGSPGE
                     ALEVQAGLEDEGRQLVLAIVAQELSEMSGTDLLASIRQAHPHSKLGLTMDWGAWLDKG
                     IAETVRESMAIGVTDFYVYKPGVSPDERFHNMVTSALLDWSREQRTVPDVIRVIGESW
                     SGRAYELRETLQRCVAPHTFSLAESEEGRALLAGVEPGTPLPLLILPDGRVLGDPTNR
                     EIAEADGSAIGIEFDDFDVVIVGAGPAGLSAAVYGASEGLRTLVIDEGGVGGQAISKV
                     ITFSSPVNG"
     gene            complement(908..>1210)
                     /locus_tag="P5138_11290"
     CDS             complement(908..>1210)
                     /locus_tag="P5138_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRV61207.1"
                     /translation="VLRGCLDSAGLLGYFDGWAFSDEVGVYKPDRQIFAHALDSIGAR
                     PEDSWHVGDLKRTDVAGSRDFGMTPVRYRGVAEDEDEASEAEHVITSHPEILDLLD"
BASE COUNT          214 a          433 c          355 g          208 t
ORIGIN      
        1 ttccattaac cggagaactg aaagtaatta cttttgagat cgcctgacct ccgaccccgc
       61 cttcatcgat gaccagggtc ctcagaccct ctgaggcgcc gtagaccgcg gccgacagac
      121 cggccggtcc ggccccgacg atcaccacgt cgaaatcatc gaactcgatg ccgatcgccg
      181 aaccgtccgc ctcggcgatt tcgcggttgg ttggatcacc caggacccgc ccgtcgggaa
      241 ggatcagcag cggcaacggc gtgcccggct cgaccccggc gagcagcgcc ctgccttcct
      301 ccgactcggc cagggagaag gtgtggggag cgacgcagcg ctggagcgtt tcccgcagct
      361 cataggcacg gccggaccag gattcaccga tcacgcggat cacatcgggc accgtccgct
      421 gctcccggct ccagtcgagc agcgccgagg tgaccatgtt gtggaaacgc tcatccggtg
      481 agactcccgg cttgtagacg tagaagtccg tgacgccgat cgccatcgat tcccgaaccg
      541 tctcggcaat ccccttgtcc agccaggccc cccagtccat ggtcaggccc agcttcgaat
      601 gtgggtgggc ctggcggatg ctcgccagca gatcggtacc cgacatctcc gatagttcct
      661 gggcgacgat cgccaggacc agctgcctgc cttcgtcttc gagcccggcc tggacctcga
      721 gtgcctcgcc gggcgacccg gaacagatca cctcgtactc ggacccgtag cgccctcgca
      781 gggcgctctc gatcaccccg agcgcttccg tgtcgtcgtc aactgccagg atcgtccgcg
      841 taaggtcagt agccacgccc gaaccttagg ccgcatcaca agccggtgtc aacccgtttg
      901 tccggcctca atccagcagg tcgaggatct ccgggtggct ggtaatcacg tgttcagcct
      961 cggatgcctc gtcttcgtct tcggccaccc cccggtaccg gaccggggtc atcccgaaat
     1021 cccttgatcc ggccacgtcg gtgcgcttca ggtctcccac atgccaggaa tcctccggcc
     1081 tggccccgat cgagtcgagc gcgtgggcga agatttgacg gtccggcttg tacacgccga
     1141 cctcgtccga gaacgcccag ccgtcgaaat acccgagcag accggccgag tcgaggcaac
     1201 cgcgcaggac
//
LOCUS       DAOTIP010000699         1208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_138508_length_1208_cov_3.138768, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000699 DAOTIP010000000
VERSION     DAOTIP010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138508_length_1208_cov_3.138768"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(334..480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            571..>1208
                     /gene="rpiA"
                     /locus_tag="P5138_11295"
     CDS             571..>1208
                     /gene="rpiA"
                     /locus_tag="P5138_11295"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HRV61208.1"
                     /translation="MKPARHREVTFEPFDAGSISNYEAKFKAAEEVAKMAESGQVIGA
                     GSGSTAFLAVRAIASRVRHGELHDIRLIPTSIESELTITGLGLVQADLAEVAPDWLFD
                     GADEVDPDHNLIKGRGGALFREKLLFEACQLRRVLIDPSKRVDQLGTNFAVPVEVVPR
                     AASVVIAGLKALGATETVIRTGSGKDGPVLTEQGNLLLDCRFESIGDGLEGE"
BASE COUNT          221 a          355 c          407 g          225 t
ORIGIN      
        1 catagccccg gtcattgaag ctggcgtggc cgccgccgta gacgtagggc ttgttccgga
       61 tccggttggc ccaggcgatg accttcttga ccctggccgg ggcgctggcc ggagcgtagg
      121 ccttgccgcg gatcagccgg gccttgcgga tcggcttgca actggtggcg ccgaccgaga
      181 gcccgccgct ggaggaggcg gacttgcagg acgcgtcggc ccgtgagggc gagccgaggg
      241 tgaagatgag gcaacaggcg agcaggcctg tcaggccgac tgcaaggctg cgaacgaacg
      301 atctctgggt ttgaggggat gaggggtaaa tgccgatctc cttttcagtg cctgcggagt
      361 tagctgacgg gctagcgctc taggagtcgc gctctcactc atccgagtgt gttcgcccca
      421 actacttggg tcccccgctt tccgggccag taggtcccgg aaattcggcg tatcggtttt
      481 cagaaacacg aagtgtagag gatttactaa cgctttgcac ataaacggcg tccgacctct
      541 cgcagatacc aatgccctag tctcttggtg gtgaaacccg cccggcatcg agaagtaacg
      601 ttcgaaccct tcgacgcagg gtccatttcc aactacgaag ccaaattcaa ggccgcggag
      661 gaagtggcga aaatggccga atccggccag gtcattggtg ccggatccgg ttccacggca
      721 tttctggcgg tgcgggcgat cgcttcgagg gtacgtcacg gcgaactcca cgacatccgc
      781 ctgattccca cctcgatcga atcggaactg acgatcacgg ggctcggtct ggtccaggcg
      841 gatctggccg aggtggcgcc ggactggctg ttcgatgggg cggacgaggt tgatcccgac
      901 cacaacctga tcaagggccg cgggggagcg ctatttcggg agaagctgct gttcgaggca
      961 tgccagctgc gacgggtgct gatcgatccg tcgaagcggg ttgaccagct gggcaccaac
     1021 tttgcggtac cggtcgaagt agtgccgcgc gccgcctccg ttgtgatcgc cggcctgaag
     1081 gccctcggcg ccaccgaaac ggtcatccgc accgggtccg gcaaggacgg gccggttctc
     1141 accgagcagg gaaacctgct gcttgactgc cgcttcgaat cgatcggtga cggcctcgaa
     1201 ggcgagat
//
LOCUS       DAOTIP010000700         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_138683_length_1207_cov_3.212674, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000700 DAOTIP010000000
VERSION     DAOTIP010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138683_length_1207_cov_3.212674"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1126)
                     /gene="ftsY"
                     /locus_tag="P5138_11300"
     CDS             complement(116..1126)
                     /gene="ftsY"
                     /locus_tag="P5138_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRV61209.1"
                     /translation="MSTTWNDILDLGDRVVPDTAPPPAAEEEAPKKIGAFRRLRESLA
                     KSRRALTEEIGSSLFDRIDDETWERLEEALILADAGATTTAQVVEKLEHEVDSGAVPP
                     DGAAIRERLIGILTEIASTNRAPINLTQDPAVVLMTGVNGTGKTTSIGKIAWHLKNEF
                     GLSVLLGAADTYRAAAAEQLSTWADRAGADLVRSNEGADPGSVAYDALSAAQARGSDV
                     VIIDTAGRLHTQSNLMDELGKVKRVIDKQIPGAPHETLISIDATTGQNGLRQAKAFAE
                     ATDVSGVVLTKLDGTAKGGITLAIATELGIPVKLIGTGEALEDLRPFDPEDFARALLE
                     DE"
BASE COUNT          167 a          408 c          400 g          232 t
ORIGIN      
        1 cgacgctgaa gggccgcggc aatcaggccg gctgtttccg cctgcgggtc cggcgctcgc
       61 tgaacctgcg ggttatcgcg gtctccggcc gggtggcgct caggcgcagg tagcgctact
      121 cgtcttcgag cagagcgcgg gcgaaatcct cggggtcgaa gggccgcagg tcctccaggg
      181 cttcaccggt cccgatcagc ttgaccggga tccccagttc ggtggcgatc gccagggtga
      241 tccccccctt tgcggtgcca tcaagcttgg tcaggaccac tccggagacg tcggtcgcct
      301 cggcgaatgc cttggcctga cgcaacccgt tctggccggt cgtcgcgtcg atcgagatca
      361 gggtttcgtg cggcgcgccg ggaatctgct tgtcgatcac ccgtttgacc ttgccgagct
      421 cgtccatcag gttcgactgg gtgtgaagcc ttccagcggt gtcgatgatc accacatcgc
      481 tgcccctcgc ctgggccgca ctgagcgcgt cataggcaac cgaccccggg tccgcccctt
      541 cgttcgagcg aaccaggtcc gcccccgccc gatcggccca ggtcgagagc tgctcggcgg
      601 cggcagcgcg gtaggtatcg gcggccccca gcaggaccga aaggccgaat tcgttcttca
      661 ggtgccaggc gatcttgccg atcgaggtcg tcttgccggt gccgttcacc ccggtcatca
      721 ggaccaccgc cgggtcctgg gtgaggttga tcggcgcccg gttggtcgag gcaatctcgg
      781 tcaggatccc gatcaggcgc tccctgatcg cggcaccatc cggcgggacc gcccccgaat
      841 ccacttcgtg ctcgagtttc tcgaccacct gtgcggtcgt ggtggcaccg gcgtcggcga
      901 ggatcagcgc ttcttccagg cgttcccagg tctcatcgtc gatccggtcg aagagactcg
      961 agccgatttc ctcggttagc gcccggcggc tcttggcaag gctttcgcgc aggcgccgga
     1021 aagcaccgat cttcttcggg gcttcctcct cggcggccgg cggcggggcc gtgtcgggaa
     1081 ccacgcggtc gccgagatcg agaatgtcat tccaggtcgt gctcacggtc gggatcttga
     1141 ctgaatcggg tgcccggcgt cagggccaga aatcctcgac caggatcccg tcctggcctt
     1201 cggcttt
//
LOCUS       DAOTIP010000701         1198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_140305_length_1198_cov_3.738408, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000701 DAOTIP010000000
VERSION     DAOTIP010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140305_length_1198_cov_3.738408"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..>1198)
                     /gene="argS"
                     /locus_tag="P5138_11305"
     CDS             complement(292..>1198)
                     /gene="argS"
                     /locus_tag="P5138_11305"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRV61210.1"
                     /translation="VEMDDFFSERSLYEDNLVDRGIERMREAGHVFDDDGAVWLRTTD
                     FGDDKDRVLIRSSGEVTYFGADVAYHVDKIERGHDRLIDVLGADHHGYVPRMAAAMEA
                     LEGGKAEFEAQIMQLIHLVEGGQRTKMSKRAGDIVTLDELTEDIGADAARWFLLQRSH
                     NQTIDLDLDLARSQSSENPVYYVQYAHARIQSILRKAAEEGGEADGDILAAPADPSEK
                     ALVSKLLEFPGQVHEAAEKRAPHRICAYASETAAAFHAFYRDCQVVGAEGEGVEAARI
                     QLCGVTAETIRQSLALLGISAPDRM"
BASE COUNT          202 a          388 c          378 g          230 t
ORIGIN      
        1 gagaggtctt tccggccatt cggcatgtag cgggcgacaa ctccggacga gcggacctga
       61 acgatccggc ccgacctggt ttgggcaata ccgagaccgg tctgcctccc ttcgaagctc
      121 acgattccat ccccggaaaa ggacttgtcc ggagaccccg cgaccggcca tttggctgcc
      181 gcggccaagg ctgcgggcaa ggccagtacg aggaggagca agacgaaact ctttttcata
      241 cctcaatgaa cacggagtga cacgggacgc ttcggttccc tggtcacccg gttacatccg
      301 gtcgggagcg ctgatcccaa gcagtgccaa tgactgcctg atcgtttccg cggtgacgcc
      361 gcaaagctgg atgcgggcgg cctcgactcc ctcaccttcg gcgccgacga cctgacagtc
      421 gcggtagaag gcgtggaatg cggcagcggt ttcactggcg taggcgcaga tccgatgcgg
      481 ggcccgcttt tctgcggctt cgtgaacctg gcccgggaat tcgagcagtt tcgagaccag
      541 ggccttctct gacgggtcgg cgggagcggc gagaatgtca ccgtcggctt cgcccccttc
      601 ctcggccgcc ttgcggagaa tgctctggat ccgggcgtgg gcgtactgga cgtaatagac
      661 cgggttctcc gacgactggc tgcgggccag gtcaaggtcc aggtcgatgg tctggttgtg
      721 gctgcgctgg agcaggaacc agcgagcggc gtctgctccg atgtcctcgg tcagctcgtc
      781 gagggtgacg atgtctcccg cccgcttgct catcttggtc cgctggccgc cttcaaccag
      841 gtggatcagc tgcatgatct gcgcctcgaa ttcggccttg cctccctcga gcgcctccat
      901 cgccgcggcc atccgcggca catagccgtg atggtcagca cccagcacgt cgatcagccg
      961 gtcatgtccc cgctcgatct tgtcgacgtg gtaggcgacg tcggccccga aataggtgac
     1021 ctcaccgctg gagcggatca ggacgcggtc cttgtcatcg ccgaagtcgg tggtccgcag
     1081 ccagacagcg ccgtcgtcgt cgaacacatg accggcttcc cgcatccgtt cgattccccg
     1141 gtcgaccagg ttgtcctcgt aaagcgaccg ttccgagaag aagtcgtcca tctcgacc
//
LOCUS       DAOTIP010000702         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_141527_length_1192_cov_2.247142, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000702 DAOTIP010000000
VERSION     DAOTIP010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141527_length_1192_cov_2.247142"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1130
                     /locus_tag="P5138_11310"
     CDS             237..1130
                     /locus_tag="P5138_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRV61211.1"
                     /translation="MSFETILYSTAGPVATITLNRPEHLNTIVPPMPDEVEQAVGEAT
                     RDQDVKVIVLRGAGRSFCAGYDFGGGFEHWNDYLATDGKWDPGKDFAMATAPELAPTQ
                     KFMSIWRTPKPVIAQVHGYCVGGGSDFALCADLIVASEDAVIGTPYSRMWGAYLSGMW
                     IYRLGLARTKFHSLTGRPLNGKQAAEVELINQAVPFDELEATVAGLAADLASIPASQL
                     AAMKLVVNQAYENMGLASTQTLGPILDGLMRNTPDALEFIDRAESEGVGSVIRDRDGD
                     FGDYSQAPPELQPDPNNVIEP"
BASE COUNT          207 a          406 c          391 g          188 t
ORIGIN      
        1 cggcgctgca ggccgacgcc gatccgtcac accacctgcg cgagctcggc gaccagcggc
       61 cgcggttccg cccggccacg atgaaaggtg cttctctccg ggccagttcg gcggcgacca
      121 gcttgccggt gtatccggtt gcgccgtaaa cagtgatcgg ctcggggatc tcgctcatgc
      181 cgggattctc gcagccgcgg ggaattccgg cgtgaagcgg cgatagggtg cggcagatga
      241 gcttcgagac gatcctctac tcgaccgcgg ggccggtagc gacgatcacg ctcaaccggc
      301 ccgagcacct gaacacgatc gttccgccga tgccggacga agtcgagcaa gcggtcggcg
      361 aggcgacccg tgaccaggac gtcaaggtga tcgtcctccg gggtgccggc cgttccttct
      421 gtgccggtta cgacttcggc ggcggcttcg agcactggaa cgattatctg gcgaccgacg
      481 gaaagtggga cccgggcaag gacttcgcga tggccaccgc gcccgagctc gctcccaccc
      541 agaagttcat gagcatctgg cggacaccga aaccggtgat cgcccaggtc cacggatact
      601 gcgtgggcgg tggcagcgac ttcgctctct gcgccgatct gatcgtggcc agcgaagacg
      661 ccgtgatcgg aaccccgtac agccggatgt ggggtgccta cctctccggc atgtggatct
      721 accggctcgg cctcgcccgc accaagttcc attccctgac cggcaggccg ctgaacggca
      781 agcaggcagc ggaggtcgaa ctgatcaacc aggcggttcc cttcgatgag ctcgaggcga
      841 ccgtcgccgg gcttgccgca gacctggcct cgattccggc gtcccagctg gccgcgatga
      901 agctcgtcgt caaccaggcc tacgagaaca tgggactggc ctcgacccag accctgggcc
      961 cgatccttga cggcctgatg cgcaataccc ccgatgccct cgagttcatt gaccgggccg
     1021 agtcggaagg ggtggggtcg gtgatccgtg accgggacgg cgacttcggc gactactcgc
     1081 aggcgccacc cgagcttcag cccgacccga acaacgtgat cgaaccctga tgccgggaag
     1141 ccgcgcccgc cgggaccaga ccgagcacta cggggcttcc acccttgagc tc
//
LOCUS       DAOTIP010000703         1187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_142454_length_1187_cov_2.007951, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000703 DAOTIP010000000
VERSION     DAOTIP010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142454_length_1187_cov_2.007951"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..>1187)
                     /locus_tag="P5138_11315"
     CDS             complement(211..>1187)
                     /locus_tag="P5138_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61212.1"
                     /translation="RLDFEQVRKDYSGPRCQHCGLARARKTTFLLRGEDGEIVQVGST
                     CLKEFLGDSDPKRALLQADLVSQARELIIAAAPAEKEDRTGRLPTVEEFLSNVLAITE
                     QEGGFVYKKNSGEDQTSTAELAIENFRMNQKNTFDPLGRPAWVEVRPEHVRQTKEKIA
                     EFTDLLSRQNEKHSDFDHRILQALEKDVAWPEKQGMLATVFERTKRLRQLETRLQGDG
                     PWLGEVGETASVWATLVWVGKPFRAPSGGESIALHFSDTETGAAITCFQPAGELPAWL
                     APRWNYPLSGTVRKHDEFKGQKVTVLDGWAIDLGGQGKMPRSANSSDT"
BASE COUNT          222 a          372 c          332 g          261 t
ORIGIN      
        1 gcgaccaact gagcgcctgc ccatcaagga tttcctcgcc ggtcagcgac tcggaggcca
       61 tgacaccggc cttccagttc agcagcccat ccacccagat ttcaaggcgg ccgtggtcgg
      121 tttcaacggc ggtctggtga accaccagct gttctctttc ggctaccaga agcattgagc
      181 aacagattag ccgcggtgaa ggccaacctc tcaggtatcc gacgaattgg ccgatctagg
      241 catcttcccc tggccgccaa ggtcgatcgc ccagccgtca agaacagtca ccttctggcc
      301 cttgaactca tcgtgctttc gaaccgttcc ggacagcgga tagttccagc ggggggcaag
      361 ccaggcaggc agctccccgg ccggctgaaa acaggtgatt gccgccccgg tttcggtgtc
      421 ggagaagtga agggcgatgc tctcgccccc ggagggtgcc cggaacggtt tgccaaccca
      481 aactagggtt gcccacacgg aggcggtctc ccccacctcg cccaaccatg gtccgtcacc
      541 ttgaaggcgt gtctcgagtt gacgtaaccg tttggttcgc tcgaacacgg tggcgagcat
      601 cccctgcttt tcaggccagg ccacatcctt ttcgagggct tgaaggatcc ggtgatcgaa
      661 atcgctgtgc ttttcgtttt gccgggaaag aagatcggtg aactcagcga tcttctcttt
      721 cgtttgacga acatgctccg ggcgaacctc aacccaggcc ggccgaccca gtgggtcgaa
      781 cgtgtttttc tggttcatcc ggaagttctc gattgccagt tccgcagtcg aggtctgatc
      841 ttcgcccgag tttttcttgt agacgaatcc gccttcctgc tcggtgatcg caagcacatt
      901 ggaaaggaac tcttcaactg tggggagacg tcccgttcgg tcttcctttt cagctggtgc
      961 ggccgcaatg atcagctccc ttgcctgcga gaccaggtcg gcttgaagaa gcgcccgttt
     1021 cgggtcgctg tcaccgagaa actctttcag gcaggtcgac ccgacctgga ctatttcccc
     1081 gtcctcgccc cttaacagga acgttgtctt gcgggccctg gccaggccac aatgctgaca
     1141 gcgcggcccc gaatagtcct tgcgaacctg ttcgaagtcc aatcggg
//
LOCUS       DAOTIP010000704         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_142565_length_1186_cov_2.805482, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000704 DAOTIP010000000
VERSION     DAOTIP010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142565_length_1186_cov_2.805482"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1186
                     /locus_tag="P5138_11320"
     CDS             <1..>1186
                     /locus_tag="P5138_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRV61213.1"
                     /translation="VKADLFTGRFLNEEVSTVPDIDLDFPRDIRERLIPRIHERYGRD
                     RSALVAAFPTYRSRGAIRDFGKVLGIPGTEIERAARAVDFHSGSDDFQRDLTQAIGRE
                     RAHSAAWKHLAWLSRQARTLPRHASQHSGGMVISTRPLIEICPVVPAAMEERQIVQWD
                     KDSCQDAGFLKIDLLGLGMLSAVERTIDEIHRIRGETVDLSRIDLDDREVFGMIKRAE
                     TTGVFQIESRAQMQMLPRMRPEDIDDLTIQVALIRPGPIQGGAVHPYVERRRKLREDP
                     GYEVPFAHDLLRPVLGETHGVIIFQEQVIEVAMHVAGFSSSEAESLRRAMSRKRSRRS
                     LEAHHQRFVEGAMANGVTREIADGIFEQIIGFSGFGFPKAHSAAFGLLAYQSAWLKHH
                     YGP"
BASE COUNT          197 a          418 c          383 g          188 t
ORIGIN      
        1 gtcaaggccg atctcttcac cggccgcttc ctgaacgagg aggtgagcac cgtgccggac
       61 atcgacctcg acttcccccg cgacatccgg gaacggctga tcccccgcat ccacgagcgc
      121 tatggccggg accggtccgc cctggtcgcc gccttcccca cctaccgttc ccgcggcgcg
      181 atccgggatt tcggcaaggt gctcggcatc cccggaaccg agatcgaacg ggccgcccgg
      241 gcggtcgact tccacagcgg gtcggatgac ttccagcgcg acctgaccca ggcgatcggc
      301 cgcgagcggg cccattcggc cgcctggaag cacctggcct ggctgtcccg gcaggcccgg
      361 accctgcccc gccacgcctc gcagcattcg ggcgggatgg tgatctcgac ccggcccctc
      421 atcgagatct gcccggtcgt cccggccgca atggaggagc gccagatcgt ccagtgggac
      481 aaggattcct gccaggacgc cggcttcctc aagatcgacc tgctcgggct gggcatgctc
      541 tcggcagtcg agcggaccat cgacgagatc caccggatcc ggggcgagac cgttgacctg
      601 agccggatcg acctcgacga ccgggaggta ttcgggatga tcaagcgggc cgagaccacc
      661 ggcgtcttcc agatcgagag ccgggcccag atgcagatgc tgccccggat gcgcccggag
      721 gacatcgatg acctgaccat ccaggtcgcc ctgatccggc ccggccccat ccagggcggc
      781 gcggtccacc cttatgtgga gaggcgacga aagctcaggg aggatcccgg ttacgaggtc
      841 cccttcgccc acgacctgct ccggccggtc ctcggcgaga cgcatggggt gatcatcttc
      901 caggagcagg tgatcgaggt ggcaatgcat gtggccggtt tcagttcctc cgaagccgag
      961 tctctgagaa gggcgatgag ccgcaaacgc tcgcggcgtt cgctggaggc ccaccaccag
     1021 aggttcgtag agggtgcgat ggcgaacggc gtcaccaggg agatcgccga cggcatcttc
     1081 gagcagatca tcggcttctc cggcttcggc ttccccaagg cccattccgc cgctttcggc
     1141 ctgctcgcct accagtccgc gtggctcaag caccattacg gtccgg
//
LOCUS       DAOTIP010000705         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_142882_length_1184_cov_4.341010, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000705 DAOTIP010000000
VERSION     DAOTIP010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142882_length_1184_cov_4.341010"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="P5138_11325"
     CDS             <1..1129
                     /locus_tag="P5138_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61214.1"
                     /translation="LLRDSSRYLTIRALRTTGTEQLNMAGSMIFLLRGNPALREFWAW
                     DEFDRAYAEPFTTEDGPALLAMTESQQGSEAAEHLERWLEVQPGAFRVMRTIEEPVRG
                     FHSELMLDVAPSEAVAADPGTAACLEYAQRRGLQPGEKLIVWRHLLDSQLGQGRSPTL
                     NLTTVINTQRILTRAPETAIELVAAFAEPDQANLDAMTAFGFRPAPEANYEIGGIRYC
                     PLVHDWRQSDLGEWLRVTGARARDFGSRTLELQVDTQPLALAREDFEIAVKGALADLH
                     RPERLARNPLLAGRLVASFPAKERPDALGALIETEIAALAGEPRGEKPQRALDRTFLR
                     PAATREAAAEALDLPFSTYRRHLAQGIQRLTERLWQLEITA"
BASE COUNT          212 a          390 c          384 g          198 t
ORIGIN      
        1 gctgctccgg gattcgtccc ggtatctgac gatccgggcg ctacgcacca ccgggaccga
       61 gcaactcaac atggcaggga gcatgatctt cctcctccgg ggcaacccgg cgctcaggga
      121 attctgggcg tgggacgagt tcgaccgggc ctacgcggag ccattcacga ctgaggatgg
      181 tccggcgctg ctggcgatga ccgaaagcca gcaaggcagc gaggcggcag agcatctgga
      241 gcgctggctc gaagtccagc ccggggcttt tcgggtgatg aggacgatcg aagaacccgt
      301 ccgcggtttc cactcggagt tgatgctcga tgtcgcacca tccgaagcgg tggcggcgga
      361 cccggggacg gccgcttgtc tggagtacgc gcaacggcgg ggcttgcagc ccggcgagaa
      421 gctcatcgtg tggcgccacc tccttgactc gcaactgggc cagggtcgtt caccgaccct
      481 caacctcacc accgtgatca acactcagcg cattttgacg cgcgccccgg agacggcgat
      541 cgaactggtc gcggcattcg ccgaacccga tcaggcgaat ctcgacgcaa tgaccgcttt
      601 cggttttcgt cctgcgcccg aggccaacta cgagatcgga gggatccgct actgccctct
      661 ggttcacgac tggcggcaga gtgacctcgg ggaatggctc cgggtgaccg gtgcccgggc
      721 ccgagatttc gggtcccgaa cgctcgagtt acaggtggat actcagccgt tggcgcttgc
      781 gcgcgaggat ttcgaaatcg ctgtcaaggg cgctctcgct gatctccacc gcccggagcg
      841 gctggcccgg aacccccttc tggccggtcg gcttgtggct tccttcccgg caaaggaaag
      901 gcccgatgcc ctcggcgccc ttatcgagac cgagattgcg gccctggccg gggagccgcg
      961 cggagaaaag ccgcaacgcg cactggacag gacattcctc aggccggcgg cgacgagaga
     1021 ggcagctgcg gaagccctgg atctcccctt cagcacctat cggcggcacc tcgcccaggg
     1081 catccagcgc ctcaccgagc gcctctggca gctcgagatc acagcctgac cattacctcg
     1141 cccgaaaagt gagcaggttt cggtctggaa agtgagcacc cctt
//
LOCUS       DAOTIP010000706         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_142902_length_1184_cov_3.270151, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000706 DAOTIP010000000
VERSION     DAOTIP010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142902_length_1184_cov_3.270151"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="prmC"
                     /locus_tag="P5138_11330"
     CDS             complement(<1..857)
                     /gene="prmC"
                     /locus_tag="P5138_11330"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRV61215.1"
                     /translation="MPASGAKAAEALAAAIDSFTAAGIETPRLDAEVLLAELAGVDRA
                     ALIADREQTLEAPVARAFSEAVRRRLRREPVAYILGKKGFRHIELAVDRRVLIPRPET
                     EMLVDLALEAGPASVLEVGTGSGAIALAVADELPGCRVTATDVSDGAIEVAVANAARL
                     GLGERVDFILGSLPPGSADFDLILANLPYVPDSDGLPPEVGGWEPEGALFGGPDGTSV
                     ISAVLEGLRDRQISAAVIGLEIGQGQGERVSALLHGAGWPETEVRLDLAGIDRVEAHF
                     GLRPACAMQ"
     gene            complement(860..>1184)
                     /locus_tag="P5138_11335"
     CDS             complement(860..>1184)
                     /locus_tag="P5138_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRV61216.1"
                     /translation="GIVVSMQDEKSQLQNREKAMRVLRARLYERQLAEQQAAVAAERK
                     AQVGTGERSEKIRTYNFPQGRVTDHRIKLTAHDLDGIMNGNLGQFTDALAAEEKRQRL
                     EAQAV"
BASE COUNT          176 a          436 c          382 g          190 t
ORIGIN      
        1 tgctgcatgg cgcaggctgg ccggagaccg aagtgcgcct cgacccggtc aattcccgcc
       61 aggtcgaggc gcacttcggt ctccggccag cctgcgccat gcagcagggc cgagaccctt
      121 tcgccctgac cctggccgat ctctagaccg atcaccgctg ccgaaatctg ccggtcccgc
      181 aggccttcga gaacggccga gatcaccgaa gtgccatccg gcccgccgaa cagggccccc
      241 tccggttccc agccgccgac ttccggtggc agtccgtccg agtccggcac atagggcagg
      301 ttggcaagga tcaggtcgaa atccgccgac cccggcggca gcgaaccgag gatgaagtcg
      361 acccgctccc ccagcccgag ccgggcggca ttggcgaccg cgacctcgat cgccccgtcc
      421 gagacatcgg tcgcggtgac ccggcagcca gggagttcat cggcaacggc cagggcgatc
      481 gccccggagc cggttccgac ctccagcacc gaggccggcc ctgcttccag tgccaggtca
      541 accagcatct cggtttccgg ccgcgggatc agcacccgcc ggtcaacggc cagctcgatg
      601 tggcggaaac ccttctttcc gaggatgtag gcgaccggct cgcgccgcag ccgccgtcgg
      661 accgcttcgg aaaaggcgcg tgccaccgga gcctccaggg tctgctcgcg atcggcgatc
      721 agcgcggccc ggtccacccc ggccagctcg gcaagcagca cctcggcgtc aagtcgcggc
      781 gtctcgattc ccgccgcggt gaacgagtcg atcgcggctg ccagggcctc ggcggccttc
      841 gccccggagg cgggcaagtt cagaccgcct gggcttcgag gcgctggcgc ttctcctcgg
      901 ccgccagcgc atccgtgaac tgacccaggt tgccgttcat gatgccgtcg agatcgtggg
      961 cggtcagttt gatccggtgg tcggtcactc gaccctgggg gaagttgtag gtacggatct
     1021 tctccgagcg ctcgccggtt cccacctggg cctttcgctc ggcggcgacg gccgcctgct
     1081 gctcggccag ctggcgttcg tagagcctgg ccctgagaac ccgcatcgcc ttctcccggt
     1141 tctggagttg tgacttctcg tcctgcatcg aaaccacgat cccg
//
LOCUS       DAOTIP010000707         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_142911_length_1184_cov_3.135518, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000707 DAOTIP010000000
VERSION     DAOTIP010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142911_length_1184_cov_3.135518"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="P5138_11340"
     CDS             <1..208
                     /locus_tag="P5138_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRV61217.1"
                     /translation="GIVEGTIRAVFIRAPWVESTGEEVEVLSTVRDHPVAVSQGNVTA
                     LSFHPELTPDDRIHRWFTELCRPG"
     gene            complement(212..643)
                     /locus_tag="P5138_11345"
     CDS             complement(212..643)
                     /locus_tag="P5138_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61218.1"
                     /translation="MIPEGKHKASRTVAAGVVAGMMLLAALSLWSVIPLVWLWIGSQF
                     SNSQFPSLGPYLIVLFGAIASILVVAWVLGRLNEVYIRLTGARDVGPIRMGWMKSLRD
                     SEKTGDPPSLIEVVIIGSVVIAFFAFIAWFFVLAGSPLPGN"
     gene            672..>1184
                     /locus_tag="P5138_11350"
     CDS             672..>1184
                     /locus_tag="P5138_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV61219.1"
                     /translation="MSGHSKWSSIKHKKGAADAKRGKLFTKLAKAITIAAREGGGDPE
                     GNPSLATAIQKAKAQSMPKANIDRAIDRGTGEGADAEVVERIQFEGYGPGGAAILVEA
                     LTDNRNRTSAEVRHAFTKHGGNLGEPGSVAWMFETHGVVVVDGEANSEDDLMGAIDAG
                     ASDVLEEDGKL"
BASE COUNT          242 a          381 c          391 g          170 t
ORIGIN      
        1 ggggatagtc gagggaacga tccgggccgt cttcatccgc gcgccctggg ttgagtcgac
       61 cggagaggag gtggaggtgc tctcgaccgt gcgcgaccac ccggtcgcgg tcagtcaggg
      121 caacgtcacc gcgctctcct tccatccgga attgaccccc gacgaccgga tccaccggtg
      181 gttcacggag ctctgccgcc ccggttgagt cctaattacc cgggaggggg gatcctgcga
      241 ggacgaagaa ccaggcgatg aaggcgaaga aggcgatcac cacggagccg atgatcacca
      301 cttcgatcag cgagggcggg tcgcccgtct tctcgctgtc cctgaggctc ttcatccagc
      361 ccatgcgaat cggtccgacg tctcgggcgc cggtcagccg gatgtagacc tcgttcagtc
      421 gtcccagaac ccaggcgacc accaggatcg aggcgatcgc cccgaacagg acgatcaggt
      481 acggacccag gctggggaac tggctgttgg agaactgcga tccgatccac agccagacca
      541 gcggaatcac cgaccagagc gaaagcgccg cgagcagcat cattcccgcc accacacctg
      601 ccgcgacggt ccttgaggcc ttgtgtttgc cctcgggaat cattgagcgc tcttgttcat
      661 agaatcacaa catgtccggt cactcgaaat ggtcttcgat caagcacaag aagggcgccg
      721 ccgacgccaa gcgcggcaag ctcttcacca aactcgcaaa ggcgatcacc atcgccgccc
      781 gcgagggcgg cggcgaccct gaaggcaacc cttcgctggc caccgcgatc cagaaggcca
      841 aggcgcagtc gatgcccaag gcgaacatcg accgggcgat cgaccgcggc accggagagg
      901 gcgccgatgc ggaggtcgtc gagcgaatcc agttcgaggg atacgggccg ggcggagcgg
      961 cgatcctggt cgaggcgctg accgataacc gcaaccggac cagcgccgag gtccggcacg
     1021 ctttcaccaa gcacggcgga aacctgggtg agcccgggtc ggtcgcctgg atgttcgaaa
     1081 cccacggggt ggtcgtggtc gatggcgagg ccaactcgga agacgacctg atgggcgcca
     1141 tcgacgcggg ggcgagcgac gtcctcgagg aagacgggaa gctc
//
LOCUS       DAOTIP010000708         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_143170_length_1183_cov_2.246454, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000708 DAOTIP010000000
VERSION     DAOTIP010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143170_length_1183_cov_2.246454"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5138_11355"
     CDS             <1..555
                     /locus_tag="P5138_11355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV61220.1"
                     /translation="EIGDGPFDAISSIGMFEHTGSDIDNYFVETAKLGKPGGLALHHG
                     ICRQHSDEESPNTFITHYVFPDGELHRVSRVIAGLERSGQELRDVENLREHYALTLRH
                     WVANLAAGQEAAIAEVGEERERVWRLYMTASFLAFERGDIGVNQILGVIPERERVALY
                     RPSPGLELARPDFVREEVSANQIG"
     gene            complement(535..>1183)
                     /locus_tag="P5138_11360"
     CDS             complement(535..>1183)
                     /locus_tag="P5138_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV61221.1"
                     /translation="EPDLFIPIAEESDLIGRIDSWIMAEAVRQAGLWNVGRDESDRIT
                     VSVNASSRQIRKPGLAAEIAELLVVNDLQPENLAIEITENILLTGSAVIDDVLREIHD
                     LGVRLALDDFGTGYSSLSYLSDFPLDAIKIDRAFVEGLGRGDPGNSAIADAITQIGRA
                     LSLLVVAEAVSTETQLQMVRDLGCSMAQGYLIAPPLGVDQATDFVRGSGLPDLIR"
BASE COUNT          212 a          390 c          382 g          199 t
ORIGIN      
        1 gagatcggtg acggcccgtt cgacgcgatc agctcgatcg gcatgttcga acacaccggt
       61 tcggacatcg acaactactt cgtcgagacc gcgaagctcg gcaagccggg tgggcttgcc
      121 cttcaccacg gcatctgccg ccagcacagt gacgaggaat caccgaacac cttcatcacc
      181 cactacgtgt tccccgacgg cgagctccac cgggtctcgc gggtgatcgc cggtctcgag
      241 cgctccggcc aggagctgcg ggacgtcgag aacctgcggg agcactatgc cctcaccctg
      301 cggcactggg tcgcgaacct ggccgccggc caggaggcgg cgatcgccga agtgggggag
      361 gagcgggaaa gggtctggcg gctctacatg actgcgagct tcctcgcctt cgagcgaggc
      421 gacatcgggg tcaaccagat cctcggggtg atccccgaga gggagagggt cgccctctac
      481 cggccgtccc ccgggctgga gttggccagg ccggacttcg taagggaaga agtctcagcg
      541 aatcagatcg ggtagtcccg aaccacggac gaaatcggtc gcctggtcga ccccgagcgg
      601 gggagcgatc aggtagccct gggccatgct gcagcccagg tcccggacca tctgtagctg
      661 ggtttcggtc gataccgcct cggccacgac cagcagcgaa agcgctctgc cgatctgggt
      721 gatcgcgtcc gcgatcgcgg aattgccggg atcgccacgt cccaggccct cgacgaaggc
      781 ccggtcgatc ttgatcgcgt cgagcgggaa atcgctcagg taactgagcg acgaatagcc
      841 ggtcccgaaa tcatccaggg caagcctcac cccgagatca tggatctccc gcagaacgtc
      901 atcgatcacg gcgctgccgg tcaggaggat gttctcggtg atctcgatcg ccaggttctc
      961 cggttgcagg tcattgacga ccaggagctc ggcgatctcc gcggccaggc ccggcttccg
     1021 gatctgacgc gacgatgcgt tcaccgagac ggttatgcgg tcgctctcgt ccctgcccac
     1081 gttccagagt ccggcctgtc gcaccgcttc ggccatgatc cagctgtcga tccggccgat
     1141 caggtcgctc tcctcggcga tcggaatgaa caggtccggc tcc
//
LOCUS       DAOTIP010000709         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_143922_length_1179_cov_2.203737, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000709 DAOTIP010000000
VERSION     DAOTIP010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143922_length_1179_cov_2.203737"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="P5138_11365"
     CDS             <1..757
                     /locus_tag="P5138_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV61222.1"
                     /translation="VQALGGAKNHMVIMPDAVVEPTVNGLIGSGFGAAGQRCMAGSVI
                     VTVGSAHDHVIPALVEESKKLHVGNGLDEGTDVGPVISCDARDRINSWIDRAVKDGAE
                     VLLDGRSPDGADPEGSYVGPTILDKVKKDDPIVSEEVFGPVLAVVNVDTLEEAIETVN
                     ASPFGNGVSIFTESASAVRKFRHDVEVGMVGVNIGVAAPVAFFPFSGWKASFLGDLHA
                     HGLDGVEFFTRKKTITSRYFSGGHESGSYFVER"
     gene            complement(846..>1179)
                     /locus_tag="P5138_11370"
     CDS             complement(846..>1179)
                     /locus_tag="P5138_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61223.1"
                     /translation="EGIRPALYFGAALGMQLREVVEGKATRDQALADYHDFSAGHQWA
                     FDWLLRAQKLLPKIHPRLVKPTLAASFRTAGSGDYAFNKYLNIAPPEFATLATRALVA
                     GRPPVATA"
BASE COUNT          200 a          377 c          402 g          200 t
ORIGIN      
        1 ggtccaggcg ctcggcggtg ccaagaacca catggtgatc atgcccgatg cggtggtcga
       61 acccaccgtc aacggcctga tcggttccgg cttcggggcc gcgggccagc gctgcatggc
      121 cggctcggtg atcgtcaccg tcggctccgc gcacgatcac gtgatacccg ccctggtcga
      181 ggagtcgaag aagcttcacg tcggcaacgg actcgacgaa ggcaccgatg tcggcccggt
      241 catctcctgc gacgccaggg accggatcaa cagctggatc gaccgggcag tcaaggacgg
      301 ggcagaggtg ttgctggacg ggcgcagccc cgacggtgcc gaccccgaag gctcctacgt
      361 cggtcccacc atcctcgaca aggtaaagaa ggacgacccg atcgtctccg aagaagtgtt
      421 cggcccggtt ctcgcggtgg tcaacgtgga cactcttgaa gaagcgatcg agaccgtcaa
      481 cgccagtccc ttcgggaacg gcgtatcgat cttcaccgaa tccgcctcgg cggtccgcaa
      541 gttccgccac gacgtcgagg ttggcatggt cggcgtcaac atcggcgtcg ccgccccggt
      601 cgccttcttc cccttctcgg gctggaaggc ctcattcctc ggcgacctcc acgcccacgg
      661 cctcgacgga gtcgagttct tcacccgcaa gaagacgatc accagccgct acttctcggg
      721 cggccacgaa tcgggcagct acttcgtaga acgctagggt cgcccggtgc ggatttcccc
      781 catatatcgg gggcaattcg cacctaccgg tcgtcaggtc agggtgacgg agtgcctgcc
      841 ggtcgtcagg ccgtcgcaac cggaggtctg ccggcgacaa gggccctggt agccagggtg
      901 gcgaactccg gtggggcgat gttcaggtac ttgttgaagg cgtagtcgcc cgagcctgcc
      961 gtcctgaagg aggcggcgag ggttggcttg accaggcggg ggtggatctt cggcaggagc
     1021 ttctgggcgc ggagcagcca gtcgaaggcc cactggtggc cggcgctgaa gtcgtggtag
     1081 tcggcgaggg cctggtcacg ggtggctttg ccctcgacca cttcgcgcag ctgcatgccc
     1141 aaggcggcgc cgaaatagag ggcgggtcgg atgccctcg
//
LOCUS       DAOTIP010000710         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_144770_length_1174_cov_3.344951, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000710 DAOTIP010000000
VERSION     DAOTIP010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144770_length_1174_cov_3.344951"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..530)
                     /locus_tag="P5138_11375"
     CDS             complement(156..530)
                     /locus_tag="P5138_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61224.1"
                     /translation="MTPEEKVAREFVSTFNRSELDAFVELLDPEVEIHGMRGLKKGPE
                     EARSWATKKPGGVQQTILIEACESAADRVLLEVKREWRWEEDDELAYTDELFWLFGMR
                     NEKVLSWRPFEDPEEARTAFDA"
     gene            complement(606..>1174)
                     /locus_tag="P5138_11380"
     CDS             complement(606..>1174)
                     /locus_tag="P5138_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRV61225.1"
                     /translation="EREAEFGEEIMRYLERTLLMQVIDSRWREHLYDMDYLREGIHLR
                     GFAQIDPLVAYKNEGYSMFQELMNGIWEEFSRLIFNVEIEIEPDQVDGSFAPPVEEPD
                     ESSFDYSGGTMEAQPSALAEVAAGATPGPAEAGGVPGVVPGGVPNVGGQADPGGATTV
                     VKSDKENIGRNDPCWCGSGKKYKKCHGA"
BASE COUNT          185 a          397 c          373 g          219 t
ORIGIN      
        1 agatccgtcg ccctgcgggc tcccccgaag gactttttcg ggtgtacccg caccggaccg
       61 ggcgaacccc caggccccgg gacgggcggc tggtggggag ttgccggatc ggggttgggg
      121 tggccgtggg cccaccacga acgccgggcc ggacttcagg cgtcgaaggc ggtccgggct
      181 tcttcggggt cctcgaaggg ccgccagctg aggaccttct cgtttcgcat gccgaagagc
      241 cagaagagct cgtccgtgta ggcgagttcg tcgtcctcct cccatcgcca ctcccgctta
      301 acttccagca ggacccggtc agcggccgac tcacaggctt cgatcaggat cgtctgctgg
      361 actccgcccg gcttcttcgt ggcccaggac cgggcctctt cagggccttt tttcagcccc
      421 ctcatcccgt ggatctcgac ctcgggatcg agcaactcga cgaaggcgtc gagctccgac
      481 cggttgaagg tcgaaacgaa ctcgcgagca accttttctt ccggcgtcaa gtaaccacca
      541 tccgggtcat acagcgagac tattcgtgaa cgactaccga gcgaaatgac ccggatgccg
      601 caaggtcagg ctccgtggca cttcttgtac ttcttgcccg aaccgcacca gcagggatcg
      661 ttccggccga tgttctcctt gtcggacttg accactgtgg tcgcgcctcc cggatcggcc
      721 tggccaccga cgttgggtac gccaccgggg acgacgcccg gcactccacc cgcctccgcc
      781 gggcccggcg tggcgccggc ggcgacctcg gcgagggccg agggctgtgc ctccatcgtg
      841 ccgcccgagt agtcgaagct ggattcgtcc ggctcctcga ccggcggggc gaaggagcca
      901 tcgacctggt cgggctcgat ctcgatctcg acgttgaaga tcaggcggct gaactcttcc
      961 cagatgccgt tcatcagctc ctggaacatc gagtagccct cgttcttgta cgcgaccagc
     1021 gggtcgatct gggcgaaacc gcggaggtgg atgccctcgc gcaggtagtc catgtcgtag
     1081 aggtgctcgc gccagcggga gtcgatcacc tgcatgagca gggtccgctc gaggtagcgc
     1141 atgatctcct cgccaaattc ggcttcgcgc tcgt
//
LOCUS       DAOTIP010000711         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_145170_length_1172_cov_2.915846, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000711 DAOTIP010000000
VERSION     DAOTIP010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145170_length_1172_cov_2.915846"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="P5138_11385"
     CDS             <1..897
                     /locus_tag="P5138_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61226.1"
                     /translation="IIAMRHRAGAEMVEAGDQNPSHPDPVADYEFRLEDKVPTVTPGE
                     LTPEILRAAILKYGCLKVTGVMDEATARSLADDIDHAFAIRQRLSEAKEVDPTRIYEE
                     FEPNEPYQVQYVERHWVALAGGVLAADSPKMFFQMVDSLEQAGVRDVVEGYLGERAAI
                     SAQKCTLRKAEPDVKGGWHQDGKFLGDVRALNVWLSLSRCGDVAPSMDIVPVRLDDFV
                     AAGVNEGDLHYQVTDETAAEAAGETGIVRPIFNPGDVLLFDEMFLHQTGSDPSMPNPR
                     YAIESWFFGPSAFPEEYAPIAF"
     gene            957..>1172
                     /locus_tag="P5138_11390"
     CDS             957..>1172
                     /locus_tag="P5138_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61227.1"
                     /translation="MGEPEFDSEIELGLEVKLPEKLPAGRADALFLYGFCFHRRQDSE
                     ALEILVNGRPNPAVGRMPRLDQFKSLHP"
BASE COUNT          207 a          388 c          373 g          204 t
ORIGIN      
        1 atcatcgcca tgcgccaccg ggccggagct gaaatggtcg aagccggcga tcagaacccc
       61 agccatcccg accccgtcgc cgattacgag ttccggctcg aggacaaggt gccgaccgtg
      121 actcccgggg agctcacccc ggagattctc cgggcggcga tcctgaagta cggctgcctc
      181 aaggtcaccg gcgtaatgga cgaggcgacc gccaggtcgc ttgccgatga catcgaccac
      241 gccttcgcca tccgccagcg gctgagcgaa gcgaaggaag ttgacccgac ccggatctac
      301 gaagagtttg agcccaacga gccttaccag gtgcagtacg tcgaacggca ctgggtggcc
      361 ctcgcgggcg gtgtgctggc ggcggactcg ccgaagatgt tcttccagat ggtcgattcg
      421 ctggagcagg ccggggtccg cgacgtggtc gagggttatc tcggcgagcg ggccgcgatc
      481 tccgcccaga aatgcaccct gcgcaaggcc gagccggatg tgaagggcgg atggcatcag
      541 gacggcaagt tcctcgggga cgtccgtgcc ctgaacgtgt ggctttccct ttcgcgctgc
      601 ggtgacgtgg ccccgagcat ggacatcgtc ccggtgcggc tcgacgattt cgttgccgcc
      661 ggagtcaacg aaggcgatct ccactaccag gtgaccgacg agaccgctgc cgaggcagcc
      721 ggggaaaccg ggatcgtgcg gccgatcttc aatcccggcg acgtccttct cttcgacgag
      781 atgttccttc accagaccgg gtcggaccct tcgatgccga acccccgcta cgcgatcgag
      841 agctggtttt tcggtccttc ggccttcccc gaggagtacg cgccgatcgc attttgatcg
      901 cgtcggcacc gcattcccga acacatcgcc cccgagttcc caatagtctc gactgaatgg
      961 gcgagcctga attcgattcc gagatcgaac tcgggctgga ggtaaagctc ccggagaagc
     1021 tcccggcagg ccgggccgac gccctcttcc tctatgggtt ctgctttcac cgccgccagg
     1081 attccgaggc cctggagatt ctggtcaacg gccgcccgaa cccggcggtg ggccggatgc
     1141 ccaggctgga ccagttcaag tccctgcacc cg
//
LOCUS       DAOTIP010000712         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_145756_length_1169_cov_2.959605, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000712 DAOTIP010000000
VERSION     DAOTIP010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145756_length_1169_cov_2.959605"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..>1169
                     /locus_tag="P5138_11395"
     CDS             166..>1169
                     /locus_tag="P5138_11395"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRV61228.1"
                     /translation="MTDLKYWDQNDQIFKTKEGKWNAVVRELVTRHETGQPILVGTVS
                     VEVSEMISNELRKQGIEHVVLNAKPEHAEREGETIAQAGRKGAVTIATNMAGRGVDIK
                     LGGDPEGMAHPQVAKQGLQPEDEGYNEAVEKIATELKPQCDAEAEEVKDLGGLFICGT
                     ERHESRRIDNQLRGRAGRQGDPGASRFFLSAEDDVIRLFAGDRIYKILDRLGPTDEEG
                     HEMPLEAKMLTKTVENAQKKVEEQNFLIRKRVLEYDDVMNEQRRVIYKYRGEILEGRD
                     MSEIAREEVDGVIGRLVDEYLAGDIIEEWDLPGLDSQLHQIWPCAIDVAAMTPESTEP
                     "
BASE COUNT          255 a          372 c          372 g          170 t
ORIGIN      
        1 atgatgaacg gcaaaatggg ggcaggtctt tccatcgtcc atgaccagat cggtgtgagc
       61 cgtgatctcg acatcgccgg gcactatgcc tatcacattc ggctcaacag caccagtaag
      121 ttggcattcg gtcttcgtgc cggcctctcc ctctactctg cgcaattgac cgacctgaag
      181 tattgggacc agaacgacca gatcttcaag accaaggaag gcaagtggaa cgcggtggtc
      241 cgcgaactgg ttacccgcca cgagaccggc cagccgatcc tggtcggcac cgtctcggtc
      301 gaggtgtcgg aaatgatctc gaacgagctc aggaaacagg gcatcgagca cgtggtcctg
      361 aacgcgaagc ccgaacacgc cgaacgggaa ggcgagacga tcgcccaggc cggccgcaag
      421 ggagcagtca cgatcgccac caacatggcc ggtcgtggcg tcgacatcaa gctcggaggc
      481 gacccggagg gcatggccca tccgcaggtg gccaagcagg ggcttcagcc cgaagacgag
      541 ggctacaacg aggcggtcga gaagatcgcc accgaactga agccgcagtg cgatgccgaa
      601 gccgaggaag tcaaggatct gggtggcctc ttcatctgcg gaaccgagcg gcatgaatcg
      661 cgccggatcg acaaccagct gcgcggccgc gccggccgtc agggtgaccc cggcgcctcc
      721 cggttcttcc tctcggccga ggacgacgtg atccggcttt tcgccggcga ccgcatctac
      781 aagatcctcg accggctcgg cccgaccgac gaggagggcc acgagatgcc gctcgaggcg
      841 aagatgctca ccaagactgt cgagaacgcc cagaagaagg tcgaagagca gaacttcctg
      901 atccgcaagc gcgtcctcga gtacgacgac gtgatgaacg agcagcgccg cgtgatctac
      961 aagtaccgcg gcgagatcct cgaaggccgt gacatgtcgg agatcgcccg cgaggaagtg
     1021 gacggcgtga tcggccgcct cgtcgacgag tacctggccg gggacatcat cgaggaatgg
     1081 gacctgcccg gcctcgactc ccagctccac cagatctggc cctgcgcgat cgacgtcgcc
     1141 gccatgaccc cggagtcgac cgagcccga
//
LOCUS       DAOTIP010000713         1164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_146682_length_1164_cov_3.853922, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000713 DAOTIP010000000
VERSION     DAOTIP010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146682_length_1164_cov_3.853922"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="P5138_11400"
     CDS             <1..675
                     /locus_tag="P5138_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61229.1"
                     /translation="LVSHEGFLNVPCPACGEPLFPWVGMPVASGIAHRCEACGLGVLS
                     HGEKFSFPGPVGSAPSESPDLDGPAFDPGSPEDAIRELELDREESGSYLFDNRASLAC
                     WVTGGAWVGLGTDRRFRFTPQAITDLIAGRDQVVTKVRWRPLRGIAITWQSGLNMFTF
                     GQNVVLGSLGKAFQVPADRSWKRGLDWFISVAVAIPAIVVALPMELIGILFRRGASAR
                     AEVQVL"
     gene            complement(683..>1164)
                     /locus_tag="P5138_11405"
     CDS             complement(683..>1164)
                     /locus_tag="P5138_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61230.1"
                     /translation="GLGAVVALEMALADPDLIDRAFLIEPPVFAALKEATEGMSADVE
                     AIRSAAAEGGEGAAYELFLSGALHTLGAGAERFGDVADRGPAAARSFLIEVPAVPAWP
                     LDPLRLASLEADVTVVTLPSTPALLKRAAEAIFERVPGAESVHSNRDGAGAVTTLLG"
BASE COUNT          172 a          388 c          403 g          201 t
ORIGIN      
        1 ctggtctcgc acgagggctt cctcaacgtg ccctgcccgg cctgcggcga gccgctcttt
       61 ccctgggtcg gcatgccggt ggcctcggga atcgcccacc gctgcgaggc ctgtgggttg
      121 ggggttctca gccacggcga gaagttctcc tttcccggac cggtggggag cgcgccttcc
      181 gaatccccgg acttggacgg cccggctttc gatccgggca gtccggaaga cgcgatcagg
      241 gagctcgagc tggatcgtga ggagagtggc tcctacctgt tcgacaaccg ggcatcgctc
      301 gcctgctggg tgaccggtgg cgcgtgggtc ggactgggta cggatcgaag gttccggttc
      361 acgccgcagg cgatcaccga cctgatcgcc ggccgggatc aggtggtgac caaagtcagg
      421 tggcgaccgc tgcgcggcat tgcgatcacc tggcagtcag ggctgaacat gttcaccttc
      481 ggccagaacg tggtgctcgg ttcgttgggc aaggccttcc aggtgcccgc ggatcgttcc
      541 tggaagcggg ggctggactg gttcatatcg gtcgccgtcg cgatcccggc gatcgtcgtc
      601 gccctgccga tggaactgat cggaattctc tttcgccgag gtgcctcggc cagggccgag
      661 gtccaggtcc tctagagcgg cctcagccga gcagggttgt cacggccccg gcgccgtcac
      721 gattcgagtg aaccgattcc gcgcccggga cccgctcgaa gatcgcctcg gccgcccgct
      781 tcagcagcgc cggggtcgaa ggaagggtga ccacggtgac gtctgcctcg agcgaggcga
      841 ggcggagcgg atccagcggc caggccggta ccgccggcac ttcgatcaga aaggaccttg
      901 ccgccgccgg tccccggtcc gcgacatcgc cgaatctttc tgcacccgct cccagggtgt
      961 ggagtgcgcc gctcaggaag agttcgtagg cggcgccctc tcccccttcg gctgccgcgg
     1021 aacggatcgc ctcgacgtcg gccgacatcc cctcggttgc ctctttcagg gcggcgaaca
     1081 ccggcggctc gatcaggaag gcacgatcaa tcaggtccgg gtcggccaga gccatctcga
     1141 gggcgaccac tgcgcccaga ccga
//
LOCUS       DAOTIP010000714         1164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_146703_length_1164_cov_3.140667, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000714 DAOTIP010000000
VERSION     DAOTIP010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146703_length_1164_cov_3.140667"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>251)
                     /locus_tag="P5138_11410"
     CDS             complement(<1..>251)
                     /locus_tag="P5138_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV61231.1"
                     /translation="YRVFIVPGLIAMAMAQSAYSNNSSTIFQARNDRYLDDILSAPMS
                     AWQVHLGYLAGGAMRSLIIGLVLTALALPFGPLAANAAPS"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<262..449)
                     /locus_tag="P5138_11415"
     CDS             complement(<262..449)
                     /locus_tag="P5138_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRV61232.1"
                     /translation="MNPLPPVEVEPHPWRGLAWLTRRECHRVLKLWKQTILAPVISSL
                     LFMVVFGLSLGDSIAEVNG"
     gene            complement(446..>1164)
                     /locus_tag="P5138_11420"
     CDS             complement(446..>1164)
                     /locus_tag="P5138_11420"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV61233.1"
                     /translation="LNLPDTPDVEAVIFGEDGITSSHGFNGVVIDMSTIDPGRTVEIA
                     AELAGRDIGFVDAPVSGGEKGAIEGNLSVMAGGSEADFARALPLLQVVGERITHVGPA
                     GAGQMVKACNQLLVGSNLMAIAEAMVLAERSGLVSPAVMREAVTGGFADSRCLQVHGQ
                     RMVDGTFEPGFKTELHAKDAGIVERMAKETGTRIAAFEAAREALDRLVETGRGELDDS
                     AVIEVVREESAEGAQPETGA"
BASE COUNT          195 a          394 c          372 g          193 t
ORIGIN      
        1 gagggagcgg cgtttgcggc gagtggcccg aagggcaggg ccagggcggt cagcaccagt
       61 ccgatgatca gcgagcgcat cgcacccccg gcgagatatc ccagatgcac ctgccaggcg
      121 ctcatcgggg cggagaggat gtcatccagg tagcggtcgt tgcgggcctg gaagatcgtg
      181 ctcgagttgt tcgaataggc cgactgggcc atcgccatgg cgatcaggcc gggcacgatg
      241 aagacccggt annnnnnnnn nccgttcacc tcggcgatgc tgtcgcccag ggaaagcccg
      301 aaaaccacca tgaagaggag ggaggagatg accggggcga gaatcgtctg cttccagagc
      361 ttcagcacgc ggtgacattc ccgcctggtc agccaggcga gtccgcgcca cggatgcggc
      421 tccacttcaa ccgggggcag cgggttcaag cgccggtctc cggctgagcg ccttctgccg
      481 actcctcgcg gacaacctcg atcaccgcgg aatcatcgag ttctccccga ccggtctcga
      541 ccagccggtc gagtgcttcc cgggccgcct cgaaggcggc gatccgggtg ccggtctctt
      601 tcgccatccg ctcgacgatg ccggcgtcct tggcgtgcag ctcggtcttg aatccgggct
      661 cgaaagttcc atcgaccatg cgttggccgt gcacctggag gcaacggctg tcggcgaatc
      721 ccccggtgac cgcctccctc attacggcgg gcgagaccaa cccgctccgc tccgccagga
      781 ccatcgcctc ggcaatcgcc atcaggttgc tgccgaccag cagctggttg caggccttga
      841 ccatctggcc ggcaccggcc ggtccgacgt gggtgatcct ttccccgacc acctggagca
      901 ggggcagggc ccgggcgaag tccgcttcgc tgcctccggc cattaccgac aggttgcctt
      961 cgatcgcgcc cttttcgccg cccgaaaccg gggcgtcgac gaacccgatg tcccgccccg
     1021 caagttccgc ggcgatttcc acggtccggc cgggatcgat cgtgctcatg tcgatcacca
     1081 cgccgttgaa accatgcgaa gaagtgattc cgtcctcacc gaagatcacc gcctcgacat
     1141 ccggcgtgtc gggcaggttg agga
//
LOCUS       DAOTIP010000715         1163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_146952_length_1163_cov_2.398917, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000715 DAOTIP010000000
VERSION     DAOTIP010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146952_length_1163_cov_2.398917"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5138_11425"
     CDS             complement(<1..285)
                     /locus_tag="P5138_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61234.1"
                     /translation="MDAVSSPDLQAERLAALKRLLPDLFSNEGKLNVDELKKLVEPAL
                     VSETERYDFRWYGKTKSKRDAFTPSRAALVYDEARSVNPDKKVTVIRREDQ"
     gene            complement(428..>1163)
                     /locus_tag="P5138_11430"
     CDS             complement(428..>1163)
                     /locus_tag="P5138_11430"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61235.1"
                     /translation="IAADEISSEEVGRETEKLRPGFAPLDLVEEISENTSLSYPTVFK
                     IVTQLENTAAMVKNPPKFLAAACAVIRNLELDEMLRSLSYRPTGESLPLTVLEQTLET
                     YFPVVPTPIRGVYDHVICDHNSVPEHDFAVAAEADSEVVCFLKLPPGYEIPTPIGMYN
                     PDFGLVLKRRALKDGKETEYYFVIETKGTGDLEDQRKLTEAERMKIKCALKHFEALGI
                     EAKLDYVPYKAPVKEYKAHFKDALPA"
BASE COUNT          176 a          328 c          375 g          284 t
ORIGIN      
        1 ctggtcctcg cggcggatga cggtgacctt cttgtcgggg ttgacggaac gggcctcatc
       61 gtagaccagc gcggcccggc tgggggtgaa ggcgtcgcgc ttggacttgg tcttgccgta
      121 ccaccggaag tcgtaccgct cggtttcgct gaccagggcc ggttccacga gcttcttcag
      181 ctcatcgacg ttcagcttgc cctcgttgga aaacaggtcg ggcaggagac gcttcagcgc
      241 ggcaaggcgc tcggcctgaa ggtcgggcga ggatacggcg tccatcgggt ttttatcttc
      301 ttcttcactc atgactctgt ctttcgtgtt ctattgacaa tctgacgctt tgccctgatc
      361 ctcgtgttgt tggccgggaa ctgcgtagcg ggtcctggcc ttttctcgtt ctgggcacca
      421 agcgctttca tgccggcaat gcgtccttga agtgtgcctt gtattccttc accggggcct
      481 tgtagggaac gtagtccagt ttggcctcga tgccgagggc ctcgaaatgc ttgagcgcgc
      541 acttgatctt catgcgttcg gcctcggtca gcttccgctg gtcttccagg tcgccggtgc
      601 ccttggtctc gatcacaaag tagtattccg tttctttgcc gtctttcagc gcccggcgtt
      661 tcaggacgag tccgaaatcc gggttgtaca tgccgatggg cgtcgggatc tcgtagccgg
      721 gcgggagctt caggaagcac acgacttcgc tgtccgcctc ggcggccacg gcaaagtcat
      781 gctccgggac gctgttgtgg tcgcagatca catggtcata caccccgcga attggcgtcg
      841 ggacgaccgg gaagtaggtt tccagcgtct gctcgagcac ggtgaggggc aggctttcgc
      901 cggtggggcg ataactcagt gaccgcagca tttcatccag ctccaggttg cggatcaccg
      961 cgcaggcggc ggcgaggaat ttgggcgggt tcttgaccat cgcggcggtg ttctcaagct
     1021 gggtcacgat cttgaatacc gtgggatagg agagggaggt gttttcgctg atttcttcca
     1081 cgagatcgag cggggcgaat ccggggcgca gcttttcggt ttcgcggccg acttcctcgc
     1141 tgctgatctc gtcggcggca ata
//
LOCUS       DAOTIP010000716         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_147110_length_1162_cov_3.023487, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000716 DAOTIP010000000
VERSION     DAOTIP010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147110_length_1162_cov_3.023487"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="P5138_11435"
     CDS             <1..972
                     /locus_tag="P5138_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRV61236.1"
                     /translation="TMGLSGVGIWGSDIGGFFAITSDTLSPELNARWVQFGAFSGIMR
                     NEADGYSVSSAHRPQVIDPDQIDNWRRYSKLRTQLYPYVSAAAADYRRTGLPMMRAMV
                     LDFPNDRKAAGLEDQYMFGPDLLVAPVIKPGQSSRKVYLPKGKWIDFWRSFSYDEPTG
                     AYDLTGAGTKNGNGWRTLPAPADQIPLLARAGSMITTISPDVTTLSLFGSDNQQVVDL
                     SDRKGRTLFAFPRGQSSDRFEKSGKLASTESDGSWKLKINDTESRPWRIKASTAVMKD
                     PFKVRCVKLNGRRLSAAAWNATASQVEVNLPSKGRNLTLLFSKKSCG"
BASE COUNT          214 a          407 c          363 g          178 t
ORIGIN      
        1 acgatgggac tttccggagt cggcatctgg ggatcggata tcggcggctt cttcgcgatc
       61 acctcggaca ccctcagtcc ggagctgaac gcccggtggg tccagttcgg cgccttctcc
      121 gggatcatga gaaacgaggc cgacggctac agcgtcagct ccgcgcaccg gccccaggtg
      181 atcgatcccg accagatcga caactggcgc cgctactcga agctccgcac ccagctttac
      241 ccgtatgtca gcgcggcggc ggccgactac cgccggaccg gcctgccgat gatgcgggcg
      301 atggtcctcg acttcccgaa cgaccgcaag gccgccggac tcgaggacca gtacatgttc
      361 ggtcccgacc tgctggtcgc gccggtgatc aagcccggcc agagcagccg caaggtctac
      421 ctgccgaagg gcaagtggat cgacttctgg cgcagcttca gctacgacga gccgaccggc
      481 gcctacgacc tgaccggcgc cggcacgaag aacggcaacg gctggcgcac ccttcccgcc
      541 ccggccgatc agatcccgct gctggccagg gctggctcga tgatcacgac catttccccg
      601 gacgtgacca cgctgagcct gttcggctcc gacaaccagc aggtggtcga cctctccgac
      661 cgcaagggcc ggaccctgtt cgccttcccc cgtggacagt ccagcgaccg cttcgagaag
      721 agcggcaagc tggcttcaac cgagagtgac ggcagctgga agctgaagat caacgacacc
      781 gaatcccggc cttggcggat caaggcatcg accgccgtga tgaaggaccc gttcaaggtg
      841 cgctgcgtga agctgaacgg ccgcaggctc tcggccgccg cctggaacgc aacggccagt
      901 caggtcgagg tgaacctgcc gtcgaagggc cgcaacctga ccctcctctt ctcgaagaag
      961 agctgcggct agaactgccg cagaagctcc cgccggtcaa tcacttccgg ggctcgccta
     1021 cgcccagttc gagctggaag tcagtaacga tccggtcccg gacggattcg atcaggcggg
     1081 ccgcgttcca cagggttccg gcggcctctt ccccggccag ccccggcgcg gtcagctggc
     1141 agaagcgtcg gatgactact gc
//
LOCUS       DAOTIP010000717         1152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_149137_length_1152_cov_2.148587, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000717 DAOTIP010000000
VERSION     DAOTIP010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149137_length_1152_cov_2.148587"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="P5138_11440"
     CDS             <1..240
                     /locus_tag="P5138_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV61237.1"
                     /translation="LTVPFLRNSPDFVFDAELLYQASHLGFRIKEVPARCRYFDEMSS
                     VGFNTGVVYGLKTLWAGFRLILHRNRILPSRKYMV"
     gene            complement(270..>1152)
                     /locus_tag="P5138_11445"
     CDS             complement(270..>1152)
                     /locus_tag="P5138_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61238.1"
                     /translation="TVGFVYLTLTVALGVTAARRPDWLRDRPVATTALAGLAGYGVLL
                     YSYFDNRSLAHVIPYISLPLLLGVTLWLWLVISNPAVPRRTAVVTLGSVLVVATLSVA
                     TVMPGAWDRGRTSLLAYAVPGGHSLKDGFHRLWNPPELKSGADQGAALLEQYMPGQET
                     SAVLTVPDLDNNILTLAGGRANSLGITDAKEQSWVPGPHVEPVAKAAEQLEPGDRMLV
                     DSSAIKAYRALVRNPEADHEEIARNNGLADIQMITLSEIAARFRLKPVAGKDGLFVVE
                     LEPRGAGAGRPAAADTP"
BASE COUNT          176 a          395 c          389 g          192 t
ORIGIN      
        1 ctgaccgtgc ccttcctccg caactcgccc gacttcgtct tcgacgccga gctgctctac
       61 caggcttccc atctgggatt ccggatcaag gaagtgccgg cccgctgccg ttacttcgac
      121 gagatgtcct cggtcggttt caacaccggg gtcgtctacg gcctgaagac cctttgggcc
      181 gggttccggc tgatcctcca tcgcaaccgg atcctgccct cgcgcaagta catggtctga
      241 ccccggtcag ggcgtcccgg tgactccggt cagggcgtgt cggcggccgc tggccggccg
      301 gccccggccc cgcgcggctc gagctcgacg acaaagaggc cgtccttccc ggcaaccggc
      361 ttgaggcgga aacgcgccgc gatctcgctg agggtgatca tctggatgtc ggccaggccg
      421 ttgttcctgg cgatctcctc atggtcggct tcggggttcc ggaccagagc ccggtaggcc
      481 ttgattgcgg aggaatcgac cagcatgcga tcgccgggtt ccagctgctc cgccgccttc
      541 gccaccggct ccacatgggg ccccggcacc cagctctgtt ccttggcgtc ggtgatgccg
      601 agcgaattgg cgcggccccc ggccagggtg aggatgttgt tgtcgaggtc cgggaccgtg
      661 agcacggcgg aggtttcctg gccgggcatg tactgctcga ggagggccgc tccctggtct
      721 gcgccggact tcagctcggg cgggttccag agacgatgga aaccatcctt cagcgagtgg
      781 cccccgggca ccgcataggc gagcaaagag gtccggcccc ggtcccaggc tcccggcatc
      841 accgtcgcaa ccgagagggt cgccacgacc agtaccgagc cgagcgtcac gaccgcggtt
      901 cgcctcggca ccgccgggtt gctgatcacc agccacagcc agagcgtgac cccgagcagc
      961 aggggcaggg agatgtaggg gatgacgtgg gccagcgagc ggttgtcgaa atagctgtaa
     1021 agcaggaccc cgtatccggc caacccggcg agggctgtgg tcgcgaccgg ccggtccctc
     1081 aaccagtccg gccggcgggc cgccgtgact ccgagtgcga cggtcagggt caggtacacg
     1141 aacccgacgg tc
//
LOCUS       DAOTIP010000718         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_149238_length_1151_cov_3.579380, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000718 DAOTIP010000000
VERSION     DAOTIP010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149238_length_1151_cov_3.579380"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5138_11450"
     CDS             complement(<1..441)
                     /locus_tag="P5138_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRV61239.1"
                     /translation="MSGPDAKSTPESKAEIDRAFEELYRDHLKDVYSYCYYRIGNHHD
                     AEDLTEQTFLQAYRHFARARRESDGRPLRPWLIRIAHNLAANYYRDRSRKPQTPLESI
                     EPPAAKHSTEDLVEGRAELRAVIENLQDLPDDRREALVMRFALGM"
     gene            complement(487..>1151)
                     /locus_tag="P5138_11455"
     CDS             complement(487..>1151)
                     /locus_tag="P5138_11455"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRV61240.1"
                     /translation="IAPKGQLPSEYVGERIRSRGVSCLGGPAHAKEAVSGSAALVLGS
                     ADGDLRAQLGDVFDRAGLVCERSRDVVGVEMAGAAKNAAVLAAAAAEPHGLNAAGIAA
                     AAVWDECVGYAVKHGADLETFNGLAGVGDLLATVMASGSRNRRAGELLGKGSAPDQIR
                     ETIGQASEALDSVPQIAATVAADGCPAEALTGLADLATGRISSDEWVAALRRSGHSRR
                     AA"
BASE COUNT          187 a          395 c          383 g          186 t
ORIGIN      
        1 catgccgagc gcgaaacgca tgaccagagc ctcgcgacga tcgtctggca gatcctgaag
       61 gttctcgatc accgcccgga gttcggcccg accctcgacc agatcctcgg tcgagtgctt
      121 cgcggccggc ggctcgatgc tctccagcgg agtctgcggt ttccgcgaac gatcgcggta
      181 gtagttcgca gcgaggttgt gggcgatccg gatcagccag gggcgaagcg ggcgaccgtc
      241 cgattcccgc cgggcccggg caaagtgccg gtaggcctga agaaatgtct gctcggtcag
      301 atcctcggcg tcgtggtgat tgccgatccg gtagtagcag taggagtaga cgtccttgag
      361 gtgatcgcgg tagagctcct cgaaggcccg atcgatctcg gccttcgact ccggagttga
      421 tttcgcgtcg gggccggaca cgcccgcacc ttatcccgaa accggcgacc tccgacccgg
      481 gcgtgctcat gccgcccgcc tcgaatgccc ggagcggcgg agcgccgcaa cccattcatc
      541 ggagctgatt cgcccggtgg cgagatccgc gaggccggtc agggcctcgg cggggcagcc
      601 gtcggccgcc acggtggcgg cgatctgggg gaccgaatcg agagcttccg aagcctggcc
      661 gatcgtttcg cggatctggt ccggggcgct gcccttgccg agcagttcac ccgcccggcg
      721 attgcgactg ccggatgcca tcaccgtggc aagcaggtct ccgaccccgg cgaggccgtt
      781 gaaggtttca aggtccgccc catgttttac ggcgtaaccg acgcattcat cccagaccgc
      841 ggcggcggcg atcccggccg cattcaggcc atgtggctcg gccgcagcgg cggccagcac
      901 ggcggcattc ttcgccgccc cggccatctc caccccgacc acgtcccgcg agcgctcgca
      961 gaccagaccg gcccggtcga acacgtcacc gagttgagcc cgaaggtcac cgtcggcgct
     1021 gccaagaacc agagcggccg aaccggacac cgcttccttt gcgtgtgcgg gaccaccaag
     1081 gcaggagacg ccgcgtgagc ggatccgctc acccacgtat tcggatggca gttgaccctt
     1141 gggggctatc a
//
LOCUS       DAOTIP010000719         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_149241_length_1151_cov_3.487226, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000719 DAOTIP010000000
VERSION     DAOTIP010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149241_length_1151_cov_3.487226"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="P5138_11460"
     CDS             <1..807
                     /locus_tag="P5138_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61241.1"
                     /translation="TRIGQSRLDDGSIPDEKIAEVAEVVETQVRLARELGAESFRAVA
                     TAALRDAPNGKEAAAEIEAVAGIPVHVVSENEEGRLSFLGATKALGYRVDGRVAVVDV
                     GGGSTEIIIGTVSGGVEQVRSWPVGSGQLAEDLISSDPPSASEVRRIRDRIDEVFSEV
                     QITQPEQAVAVGGSATSLRKIVGARLEYETLERSIRVLCGDDAVEVGRQFELDPIRVR
                     MLPTGVLILEKVSELLGQDLQIGKGGIREGIVLDMLNGGIDVSEPALAPG"
     gene            836..>1151
                     /locus_tag="P5138_11465"
     CDS             836..>1151
                     /locus_tag="P5138_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRV61242.1"
                     /translation="MSSPDSRFRLAASQVLEERATQITVFAKGVLDVTEPSAAEDLWL
                     ASRRLRAALEVFRPCLGRSEYREGRSEARRISRAVGERRDIDVAIAAYESIREETAQE
                     EAE"
BASE COUNT          196 a          350 c          419 g          186 t
ORIGIN      
        1 acccggatcg ggcagtcccg cctcgatgac ggcagcatcc ccgacgagaa gatcgccgag
       61 gtcgccgagg tggtcgaaac ccaggtgcgt ctcgcccgtg aactgggcgc cgaaagcttt
      121 cgcgccgtcg ccaccgcagc cctgcgggat gctcccaacg gcaaggaggc ggccgccgag
      181 attgaggcgg tggcgggtat accggttcac gtggtcagcg agaacgagga gggacggctt
      241 tcctttctcg gcgccaccaa ggcgctcggc tacagggtcg acgggcgggt cgccgtggtc
      301 gacgtcggcg gcggatcaac cgaaatcatc atcggcacgg tcagcggagg ggtcgagcag
      361 gtccggtcct ggccggttgg ctccggccag ctcgccgagg atctgatctc ctccgatccg
      421 ccttcggctt ccgaggtgcg gcggattcgt gaccggatcg acgaggtctt ctcggaagtc
      481 cagatcaccc agcccgagca ggcggtggcg gtcggcggtt cggcaacctc gctgcggaaa
      541 atcgtcggag cgcggctcga gtacgagacg ctggaacgaa gcatccgggt cctctgcggt
      601 gacgacgcgg tcgaggtcgg ccggcagttc gaactggatc cgatccgcgt gaggatgttg
      661 ccgaccggcg tgctgattct cgaaaaggtc tcggagctgc tcggccagga cctgcagatc
      721 ggaaagggcg ggatccgcga agggatcgtc ctggacatgc tgaacggcgg aatcgacgtt
      781 tcagaaccgg ctctcgcgcc gggttgagct tcgttcgcca ctagggtttc aggcgatgag
      841 tagtcccgac tcccgtttcc ggctagccgc atcgcaggtg cttgaggagc gggcgaccca
      901 gatcacggtc ttcgcaaagg gagtgctcga tgtgaccgag ccctcggcgg ccgaggatct
      961 ctggctggca tcccgccgcc tccgggcggc actggaggtg ttccggccct gtctcggaag
     1021 gtccgagtac cgggagggac ggtccgaggc ccggcgcata tcgagggcgg tcggcgagcg
     1081 acgggacatc gacgtggcga tcgccgccta cgagtcgatc cgtgaagaga ccgctcagga
     1141 ggaagcagaa g
//
LOCUS       DAOTIP010000720         1149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_149634_length_1149_cov_2.910420, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000720 DAOTIP010000000
VERSION     DAOTIP010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149634_length_1149_cov_2.910420"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1149)
                     /locus_tag="P5138_11470"
     CDS             complement(<1..>1149)
                     /locus_tag="P5138_11470"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRV61243.1"
                     /translation="IADLIAFLVPFLLLSGLALGGGGYDLFARHLTGILVWVLVAVLL
                     LGPWPDRIRPGRSLFLVGGLLLSLSLLSAISSIWSSSVSSSLAEFERGIGYLGIFTAS
                     YLTMRTPKQREWFARGIGAGLAVIVLLALGDRLIPGSNRDLEEGLVRLTYPLGYWNGD
                     GISCGAAAVLFTWFAAIAGNRWWRSGCLSIGILGATALYLTYSRGGLLVAVIAMILLL
                     FLSTRRLRVMATLVVVVAGTTLLLLVVSMYPAISGDKVGDPTAGESLIVILAAVVAMA
                     LAAAALEGLVRIARKRGRLVQGALETSRDRRLLLSLAGAAVAFLLVFSLAFGNNVWDQ
                     FTVSDVPAPSDAKSRFADLSGSGRYEFNRVALDIFADNPVLGSGAGTY"
BASE COUNT          252 a          378 c          370 g          149 t
ORIGIN      
        1 gataggtgcc ggcaccggag cccagtacgg gattgtcggc gaagatgtcc agcgccaccc
       61 ggttgaactc gtatcggccc gacccggaga gatcggcgaa gcgcgatttc gcgtcggaag
      121 gagccggcac gtcgctgacc gtgaactggt cccagacgtt gttgccgaag gcgagcgaga
      181 agaccagcag gaaagcgacc gcagcccccg ccagtgaaag cagcagccga cggtcacggg
      241 aggtctccag cgctccctgg acgagccggc cacgtttccg ggcgatccgg accagcccct
      301 cgagggcggc tgcggccagg gccatcgcca ccactgcggc gaggatcacg atcaggcttt
      361 ccccggccgt cggatcaccg actttgtcac cggaaatggc cggatacatg ctcaccacca
      421 ggagcagcag cgtcgttcct gccaccacga ccacgagggt ggccatgact ctcaggcggc
      481 gggtcgagag gaagagcaac aggatcatcg cgatcacggc aaccagcagc ccgccccgcg
      541 aataggtcag gtacagggcc gtggcaccga ggatcccgat tgacaggcaa ccgctccgcc
      601 accatcggtt tccggcgatg gcggcgaacc aggtgaacag gaccgcggcc gcaccgcagg
      661 agatcccgtc gccgttccag tagccgaggg ggtaggtgag acggaccagg ccctcctcga
      721 gatcccggtt actgcccggg atcagccggt cgcccagggc gagcagcacg atcaccgcaa
      781 gtccggcccc gatgccgcga gcgaaccatt cgcgctgttt cggggttctc atcgtgaggt
      841 aactggctgt gaagattccg aggtaaccga ttcctcgctc gaattcagcc aggctcgacg
      901 agaccgagct agaccagatc gatgaaatcg ccgaaagcaa agacagggac agaagcagcc
      961 cgccgaccag gaagagcgat cttcccggcc ggatcctgtc cggccaggga cccaggagca
     1021 gcaccgcaac cagcacccag acgaggatcc cggtcaggtg ccgggcgaac aggtcgtatc
     1081 cgccaccacc gagcgccagt ccgctcagga gcagaaaagg aaccagaaat gcgatcagat
     1141 cggctatct
//
LOCUS       DAOTIP010000721         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_149946_length_1148_cov_1.902104, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000721 DAOTIP010000000
VERSION     DAOTIP010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149946_length_1148_cov_1.902104"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5138_11475"
     CDS             complement(<1..204)
                     /locus_tag="P5138_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61244.1"
                     /translation="MNAISAVTVVVPAPPSVPQTATSGGPAGSVFRAGAVCGSVSEGG
                     LPGLSGAVAVSGSAVITKLISMPA"
     gene            194..>416
                     /locus_tag="P5138_11480"
     CDS             194..>416
                     /locus_tag="P5138_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61245.1"
                     /translation="MAFIASRQNLGPVLLTDLGGPSSSLAAMLNKRARYSLASAANAH
                     RAGLFATNGKAPFALVNEQLRLMAKEPDAL"
     assembly_gap    417..426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <427..850
                     /locus_tag="P5138_11485"
     CDS             <427..850
                     /locus_tag="P5138_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61246.1"
                     /translation="QVSVDPDPALRDLLIDSQEDHFATFIDCGRLEQPAEQQVASLAT
                     HIVWVSGGTLTSARKARAKITSLGFTGSAMSILYVRGETDSVPARSAQEELGKAADES
                     GLAIVVSPPVGDFVSQGLVPTASRAMKPLSAALERILG"
BASE COUNT          212 a          396 c          337 g          193 t
ORIGIN      
        1 ggccggcatc gagatcagct tggtgatcac cgcggaaccg gacaccgcga ccgctcctga
       61 cagtcccggc aggccacctt ctgaaactga gccgcaaaca gcgccggctc gaaagactga
      121 cccggccggt cctccgctag tcgccgtctg cggcaccgaa ggcggggccg gcaccaccac
      181 cgtcaccgcg ctgatggcgt tcatcgcctc acgccagaac ctcggaccgg tccttctcac
      241 cgaccttggc gggccgtcct cctcactggc agcgatgctc aacaaacggg cccgctactc
      301 gctggcctcg gccgccaacg cgcaccgggc cggactgttt gccaccaacg gcaaggcacc
      361 cttcgccctc gtaaacgaac agcttcgcct gatggccaag gaacccgatg cgctcgnnnn
      421 nnnnnnccag gtcagcgtcg accctgaccc ggccctgcga gacctcctga tcgactccca
      481 ggaagaccac ttcgccacgt tcatcgactg cggccgcctc gagcagcccg ccgaacaaca
      541 ggtcgccagc ctcgcaaccc acatcgtctg ggttagtggc ggaacgctca cctccgcccg
      601 gaaagccagg gcgaaaatca ccagtctcgg attcaccggt tcggccatgt cgattctcta
      661 tgtccgaggg gaaaccgatt cggtgccggc aaggtcagcc caggaagaac tcggcaaggc
      721 agccgacgaa tcgggactgg cgatcgttgt ttccccgccg gtcggtgact ttgtctccca
      781 aggcctggtc ccgaccgcgt cccgggcaat gaaaccgttg tcggcggcac tggaaaggat
      841 tctcggttga cgggcccggc agcaacaacc aagggtttgc gcttatccgg tatctctcac
      901 cgttcaggct caaacgaccg ggcggcgggt ggaaggcagg tcgctctcgc aaccctcggg
      961 gtcttcgcga tcgcgctcat cgcaggggtt gtggtcccga ccggattgga agttcccgca
     1021 agggacagct ctatctccgg gttgctcacg atccttcggt caaaccttgt gttgctggcc
     1081 gtgatcgggt catgcgccgg acttcaaaca tttgctcgcc gggaagtcga ggaaggaagc
     1141 cagccatg
//
LOCUS       DAOTIP010000722         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_150868_length_1143_cov_4.056066, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000722 DAOTIP010000000
VERSION     DAOTIP010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150868_length_1143_cov_4.056066"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="P5138_11490"
     CDS             <1..633
                     /locus_tag="P5138_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRV61247.1"
                     /translation="LVIEAVTENLDTKLEIWKQLDGIVKSEAYFATNTSSLPVINQAA
                     ATTRPTNFLGLHFFNPVQRMPLLEVIQSVTTSPEAMGVGLAYGEKLKKTTVETKDKAG
                     FIVNRLLVPYMLDAIRGLEEGIGSIDEIDAAMKAGAAHPMGPLTLADFVGLDTLHSIA
                     DVMFDEFRERRFAAPPTLRKMVAAGFFGVKSGRGFYDYSGEKPVPVNPGL"
     gene            733..>1143
                     /locus_tag="P5138_11495"
     CDS             733..>1143
                     /locus_tag="P5138_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRV61248.1"
                     /translation="MTKVDLDPALKAALGGGPERHHSKTAEQGKLPVRERVDLLLDEG
                     SFVEEALLANWDQEGLGADGVVTGTGLVEGRPVALMANDPTVKAGSWGPKTVEKILRI
                     QERALKLEIPMVYLVDSAGARITEQVQMFPGRRGA"
BASE COUNT          207 a          396 c          359 g          181 t
ORIGIN      
        1 ctcgtgatcg aagcggtcac cgagaacctc gacaccaagc tggagatctg gaagcagctc
       61 gacggaatcg tcaagtccga ggcctacttc gcgaccaaca cctcatcact gccggtcatc
      121 aaccaggctg ccgcgaccac gcgtccgacc aacttcctcg gcctccactt cttcaacccg
      181 gtccagcgta tgccgctgct cgaggtgatc cagtcggtca ccacttcgcc cgaggcgatg
      241 ggcgtcggcc tggcctacgg cgagaagctc aagaagacca cggtcgagac caaggacaag
      301 gcgggtttca tcgtcaaccg cctgctggtc ccctacatgc tcgatgcgat ccggggtctc
      361 gaagagggca tcggctcgat cgacgagatc gacgccgcga tgaaggccgg cgcagcccac
      421 ccgatgggcc cgctcaccct ggccgacttc gtcggtctgg acacactcca ttcgatcgcc
      481 gacgtcatgt tcgacgagtt ccgcgagcgc cgcttcgccg ccccgccgac cctccgcaag
      541 atggtcgccg ccggtttctt cggcgtcaag tcgggccgcg gcttctacga ctactcaggc
      601 gagaagccgg tcccggtcaa cccgggcctc tagccggccg ggttcatctg aaattcggaa
      661 ggtgccgcag gtttgcgacc gtcacggttc caaaccggcg gcacctccct gaaactcgtc
      721 tagtttgaag cgatgaccaa ggtcgatctc gatcccgctc tgaaggccgc cctcgggggc
      781 ggcccggagc gccaccactc caagaccgcc gagcagggca agctgcccgt ccgcgagcgg
      841 gtcgacctgc tcctcgacga gggcagcttc gtcgaggagg cgctgctggc caactgggac
      901 caggaagggc tcggtgccga cggcgtcgtg accggaaccg gactggtcga gggccgtccg
      961 gttgccctga tggccaacga ccccacggtc aaggccggat cctggggccc gaaaacggtc
     1021 gagaagatcc tccgcatcca ggagcgggcc ctgaagctgg agataccgat ggtctacctg
     1081 gtcgactctg ccggggcccg gatcaccgag caggtccaga tgttcccggg gcggcgcgga
     1141 gcg
//
LOCUS       DAOTIP010000723         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_150891_length_1143_cov_3.241728, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000723 DAOTIP010000000
VERSION     DAOTIP010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150891_length_1143_cov_3.241728"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1097)
                     /locus_tag="P5138_11500"
     CDS             complement(180..1097)
                     /locus_tag="P5138_11500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRV61249.1"
                     /translation="MDLSYCVVNNDGRDYLMACLAAIDRFHPDGLSREVLVLDNASSD
                     GSVEAVREKFPQAEVIPLEQKQGKAANDSFLLELARGRYCLLLNEDSELRAGAVEALV
                     EALDTDPEAGAAGAQLFDGDGNAYACAWRFPGVESAVVGAMFLHSRLTVESKGEETRR
                     VDWAQSSALMVRREAADEIGFLDEQFFVYYDECDFCKRLADAGWSTLYVPSAEAVHHN
                     QLSTDLTSGLPRIVEFHRNRDLYMRKHHGRAAALAVRVLTAWAYGLRTIASVFMPNAP
                     TPVYAAHARQALFPYRGESLADRASQARA"
BASE COUNT          191 a          348 c          415 g          189 t
ORIGIN      
        1 cgaaggtgct cgaggcgcgg gaagtcggaa aggcagccgg cgaggagggc gaagggatcg
       61 acgtttcagg cagacccggc cgccgcggcc tgcccgaggc aatcgaagtc gaacagtggg
      121 acgaggacct tcgctcctga aggcgcaacg ggaaaccgtc gaaatttccc ggaaagttgt
      181 tacgccctgg cctggctggc gcggtcggcg aggctttctc cccggtacgg gaagagggcc
      241 tgcctggcgt gggcggcgta gaccggggtg ggggcgttcg gcatgaagac cgaggcgatg
      301 gttctcaggc cgtaggccca ggcggtgagg acccgcaccg ccagggcggc tgcccggccg
      361 tggtgctttc gcatgtagag atcgcggttg cggtgaaact cgacgatccg gggcagacct
      421 gaagtgaggt cggtcgatag ctggttgtga tggaccgcct cggcggatgg tacgtagagg
      481 gtgctccagc cggcatcggc caggcgcttg cagaaatcgc attcgtcgta atagacgaag
      541 aactgctcgt cgaggaagcc gatttcgtcg gccgcttcgc ggcggaccat cagggccgag
      601 gactgggccc agtcaacccg ccgggtttcc tcgcccttgc tctcgacggt caggcgggaa
      661 tggaggaaca tcgccccgac caccgccgac tcgaccccgg ggaagcgcca ggcgcaggcg
      721 taggcattgc cgtcgccgtc gaagagctgg gcgccggcgg ccccggcctc ggggtcggta
      781 tcaagcgctt cgaccagggc ttcgaccgca ccggcccgga gctccgagtc ttcgttcagc
      841 aacaggcagt accggccccg ggccagctcg agcaggaacg agtcgttggc cgccttgccc
      901 tgcttctgtt cgagcgggat cacctctgcc tgcggaaatt tctcccgcac tgcttcgacc
      961 gaaccgtcgc tggaggcgtt gtccaggacc aggacttccc ggctcagtcc gtccggatgg
     1021 aagcggtcga tcgcggccag gcaggccatc aggtaatccc ggccgtcgtt gttgacgacg
     1081 cagtagctca ggtccacttc tgtgccttgg cttgcagggt cgggtgccgg ggaggggaag
     1141 ttc
//
LOCUS       DAOTIP010000724         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_150985_length_1143_cov_2.111213, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000724 DAOTIP010000000
VERSION     DAOTIP010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150985_length_1143_cov_2.111213"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="P5138_11505"
     CDS             complement(<1..543)
                     /locus_tag="P5138_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRV61250.1"
                     /translation="MTGERPAGGPGADAASACPDCLRRSWLLARLGAWIQNVVDDRDG
                     HRTPELLRLADEDLVQAVAAKRADEILAWNRSLTEAGMRSALEKAECWACCRHHEAFP
                     AGLDDGADAPVALIGRGDGIPLAEIRLENSVTVVGARKATGYGLEVAASIGREVAAAG
                     INVISGMALGIDGAVHRGALE"
     gene            complement(540..>1143)
                     /locus_tag="P5138_11510"
     CDS             complement(540..>1143)
                     /locus_tag="P5138_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV61251.1"
                     /translation="LFLDELCEFSRPSLEALRELLETGSVTISRARGRQALPCRFTLV
                     AAANPCPCGRGEDDPRCRCTEMAARRYRAALSGALVDRIDLVVAVSQPSAEALAGPGG
                     ESSTAVRERVITARELMAGRLGEGRTNSGATPEEAGQFKVTAAAAGLLAEAHQTWRLS
                     GRAHGRILRVARTVADLAGSTAVGEEHMASAIQFRRRSAQ"
BASE COUNT          163 a          439 c          364 g          177 t
ORIGIN      
        1 ttccagcgcc ccgcggtgta ccgccccgtc gatccccagg gccatgccgc tgatcacgtt
       61 aattcccgcc gccgctacct cccggccgat cgaggcggcc acctccagtc cgtagccggt
      121 tgccttgcgc gcaccgacca cggtcaccga attctccagg cggatctccg ccagcgggat
      181 cccgtcgccc cgacctatca atgccaccgg ggcatcggcg ccgtcgtcca gtcctgccgg
      241 gaacgcctcg tggtgccggc aacaggccca gcattcggcc ttttcgagtg cggagcgcat
      301 cccggcttcc gtcagcgacc ggttccaggc caggatctcg tcggcccgct tcgccgccac
      361 cgcctggacc aggtcttcgt cggcgagacg cagcaattcg ggtgtgcgat gaccgtcgcg
      421 gtcgtccacc acgttctgga tccaggcccc gagccgggcg agcagccagc ttctgcgcag
      481 gcagtcgggg caggcagaag cggcgtcagc ccctggacct ccggcaggcc tctcgccggt
      541 cattgggccg acctccggcg aaactggatc gccgatgcca tgtgttcttc cccgaccgcg
      601 gtcgatcccg cgaggtccgc aacggtgcgg gcaaccctga gaatccgccc gtgggcgcga
      661 ccgctgagcc gccaggtctg gtgggcttcg gccagcaatc cggcggcagc ggctgtcacc
      721 ttgaactggc cagcctcctc cggcgtcgcc ccggagttgg tgcggccctc ccccagccgt
      781 cctgccatca gctccctggc cgtgatgaca cgttccctga ccgccgttga cgactctccc
      841 cccggccccg caagcgcctc ggccgaaggc tggctcaccg ctaccaccag gtcgatccgg
      901 tcgaccaggg cgccgctcag cgcggcccgg tagcggcggg cggccatttc ggtgcagcgg
      961 catcggggat cgtcttcgcc ccttccgcag ggacaggggt tcgccgccgc caccagggtg
     1021 aatcggcagg gcagggcctg cctcccccgg gcacgggaaa tcgtcaccga acccgtttcc
     1081 aggagctcac gaagcgcctc cagcgaaggg cggctgaact cgcagagttc gtcgaggaaa
     1141 agc
//
LOCUS       DAOTIP010000725         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_151275_length_1141_cov_3.917127, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000725 DAOTIP010000000
VERSION     DAOTIP010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151275_length_1141_cov_3.917127"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1141
                     /locus_tag="P5138_11515"
     CDS             25..>1141
                     /locus_tag="P5138_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HRV61252.1"
                     /translation="MRRLLATTLLFLACLFGLAGQASGHALLKSSSPARGAQLKDAPE
                     HVDFTFDEPVEASLGAVRVFNTSGDQVQTDDLERPGDNADTVGAGLPADLPDGLYTAT
                     YHVVSADSHPVSGGITFTVGKPGRDLGGFTQSKTISELLAESEAGKVTEVAFWADRWI
                     GYLAIALAVGALAWMLLVWRPESTTAGIDRSPVERSFRKLVLVVSAAGLLASLLAIAF
                     QGSIGAGTSFWHAFGSGIPGEVIHTRFGSVMLVRSAIWLLLIGLVLTGGRHFSRLGPV
                     SLLALGAGLALAVTPAFAGHASTRDPGWLLVPSDIVHVSAMAVWTGGLASMLLLLPAA
                     TRQLGEPADRTGLLTRAALRFSTIALVAVALIGVSGAV"
BASE COUNT          147 a          393 c          384 g          217 t
ORIGIN      
        1 gagcggggag gccaaagaag cctggtgagg cggcttctcg ccacaactct gcttttcctg
       61 gcctgccttt tcggcctggc gggccaggcc tccggccacg ccctgctcaa gagctcctcg
      121 cccgccaggg gtgcccagct gaaggacgcg ccggagcacg ttgacttcac gttcgacgag
      181 ccggtcgagg cttcgctcgg ggcggtccgt gtattcaaca cctcgggcga tcaggtgcag
      241 accgacgacc ttgagcgtcc cggtgacaat gccgacaccg tcggggcggg cctgccggcc
      301 gaccttcccg atggccttta caccgccacc taccacgtgg tatcggccga ttcccatccg
      361 gtttccggtg gcatcacctt cacggtcggc aagccgggca gggacctggg cgggttcacc
      421 cagagcaaga cgatctccga actgctggcc gagagcgaag ccggcaaggt gaccgaggtc
      481 gccttctggg cggaccgctg gatcggctac cttgcgatcg ctctggcggt cggcgcgctg
      541 gcgtggatgc ttctggtctg gaggcccgag tcgaccaccg ccggtatcga ccggagcccc
      601 gtcgagcgga gcttccggaa actcgtgctc gtggtctccg cggcgggact tctggcctcg
      661 cttctggcaa tcgccttcca gggatcgatt ggcgcgggaa ccagcttctg gcatgccttc
      721 ggctccggca tcccggggga agtgatccac acgagattcg gctcggtgat gctggtccgg
      781 tcggcgatct ggttgcttct gatcgggctc gtcctgacgg gaggtcgcca cttcagccgc
      841 ctgggaccgg tttccctgct ggcgctgggg gccggcctcg cactggcggt cactccggcc
      901 ttcgccggcc atgcctcgac cagggacccc ggatggctcc ttgttcccag cgacatcgtt
      961 cacgtttcag cgatggcggt ctggaccgga ggcctcgcct cgatgctcct gctgcttccg
     1021 gccgcgaccc gtcagctcgg ggagccggcg gaccggaccg gtctgctgac ccgggccgcc
     1081 ctgcggttct cgactatcgc ccttgttgct gtcgccctga tcggcgtctc gggagcggtc
     1141 c
//
LOCUS       DAOTIP010000726         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_151530_length_1140_cov_2.710599, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000726 DAOTIP010000000
VERSION     DAOTIP010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151530_length_1140_cov_2.710599"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="P5138_11520"
     CDS             complement(<1..635)
                     /locus_tag="P5138_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV61253.1"
                     /translation="MPDGSDNAIRLRNLGRDYGERPALEGIELDLPEGSSLAVLGPNG
                     SGKSTLLRVLATLLRPSRGEVEVLGCSLPNDSWKLRGRIGYLGHEPLLYEDLTGRENL
                     EFQARLYGIPAGLAAERIESDLGTLGMGRRANYRVSDYSAGMKQRIAICRAVLHRPAL
                     LLLDEPESNLDPEGREAARSLIGHPGQLAWGSEAPVTRVAVSHEPEKAREES"
     gene            783..>1140
                     /locus_tag="P5138_11525"
     CDS             783..>1140
                     /locus_tag="P5138_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61254.1"
                     /translation="MKRSKRYLFVGLLTAAIAAAGLASSPAIAKIKPRNGDYVQEWNN
                     GTAAAIQTDRGRIWHIGVALRFKLPNGTPCSPGSLPSYDGVVVLDPALPNTTIGLNRR
                     SGFRFTEIRRKGFSPFL"
BASE COUNT          195 a          390 c          367 g          188 t
ORIGIN      
        1 gattcttccc gcgccttctc cggctcgtgg gagacggcga ccctggtgac aggggcttcg
       61 ctgccccagg cgagctggcc cgggtgcccg atcagggaac gggctgcctc gcggccttcc
      121 gggtcgaggt tggactccgg ctcgtcgaga agcagaagag cggggcgatg aagcaccgcc
      181 cggcagatcg cgatccgctg cttcatgcct gccgagtagt cactgacccg gtagttggcc
      241 cgcctgccca tccccagcgt cccgaggtcg gattcgattc tctccgccgc cagtccggcc
      301 ggaatcccgt agagccgggc ctggaactcg aggttctcgc ggccggtcag gtcttcgtag
      361 agcagcggct cgtggccgag ataaccgatc cggccccgaa gcttccagga gtcgtttgga
      421 agcgagcagc ccagcacttc cacctcgccg cgactgggcc tgagcagggt cgccagaacc
      481 cggagcaggg tgctttttcc ggatccgttc gggcccagga ccgccagcga cgaaccctcg
      541 ggcagatcca gttcgatccc ctccagggcc gggcgttcac cgtagtcccg gccgagattc
      601 ctcagccgga tcgcgttgtc agacccatcg ggcatccggc gcagtctccc acggatgcgc
      661 tcgcgccatt ccctcccccg gacggccgcc gggcatcgcc cagaacccga cttgcagtct
      721 gcaagccgaa tcgcgacaat caggtgagat caggggaacc gcccgttttc ggaggaggaa
      781 gaatgaaacg gagcaagagg tatctgttcg tggggttgct gaccgccgcg atcgctgcgg
      841 cagggttggc gtcaagcccg gcgatcgcga agatcaagcc cagaaacggc gactacgttc
      901 aggaatggaa caacggcacc gccgccgcga tccagaccga tcgtggccgg atctggcaca
      961 tcggcgtagc gctgcgattc aagctgccga acgggacacc ctgcagcccc gggagcctgc
     1021 cctcctacga cggtgtagtg gtgctggacc cggctcttcc caacacgacc atcgggctca
     1081 accggcggag cggcttccgc ttcaccgaga tccggcgcaa gggtttctct cctttcctga
//
LOCUS       DAOTIP010000727         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_151949_length_1138_cov_2.821791, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000727 DAOTIP010000000
VERSION     DAOTIP010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151949_length_1138_cov_2.821791"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /gene="rpsO"
                     /locus_tag="P5138_11530"
     CDS             <1..166
                     /gene="rpsO"
                     /locus_tag="P5138_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRV61255.1"
                     /translation="INDLTGHLREHGKDHHSRRGLLMMVGKRRRLLRYLERTDVERYR
                     ALVADLGLRH"
     gene            163..615
                     /locus_tag="P5138_11535"
     CDS             163..615
                     /locus_tag="P5138_11535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRV61256.1"
                     /translation="MIEVGAKAPEFTLPDHAGQEVSLSDFAGRRVLLAFYPLDFSPVC
                     SDQLARYRGIQAELDAADVTMFGISVDSAFAHKAFRDHLGIETSLLADFEPKGAVAEA
                     YGAYLGPAGHSNRSLVLIGPDGVVEWVHETDTPLDVPGPNLILEAIRA"
     gene            complement(612..>1138)
                     /locus_tag="P5138_11540"
     CDS             complement(612..>1138)
                     /locus_tag="P5138_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61257.1"
                     /translation="PSVVDTLTEPLPQPSPGLEDRVMAGMRDDEPVAQPKTGRRPWRI
                     ALPAIGALAAVGIALVLALGGGNGGDGTASAGQQFAFTSLPPGVSIAGNLEPKATGTG
                     IEVKVDGMKPGTLCRVFVKTANGKLEPAGTFRYRYDVDEGESYPATLSTAWDLSEIDA
                     LVIKAGPEIFRTGI"
BASE COUNT          176 a          406 c          357 g          199 t
ORIGIN      
        1 catcaacgat ctcaccggcc acctgcgcga gcacgggaag gatcaccact cccgccgcgg
       61 cctgctgatg atggtcggca agcgtcgccg cctgctgcgc tacctcgagc ggaccgacgt
      121 cgagcgttac cgcgccctcg tcgccgatct cggcctgcgc cattgatcga agtcggggcc
      181 aaggccccgg aattcaccct tcctgaccac gccgggcagg aagtctccct gtcggacttc
      241 gccggccgac gggtactgct cgccttctat ccgctggatt tcagcccggt ctgctcggac
      301 cagctggcca gataccgcgg catccaggcc gagctcgacg ccgccgacgt gaccatgttc
      361 ggcatcagcg tggattccgc cttcgcccac aaggctttcc gcgaccatct cggcatcgag
      421 acttcactgc tggcggattt cgagccgaag ggcgctgtcg ccgaggccta cggcgcctac
      481 ctcggcccgg ccggccattc caaccggtcc ctggtcctga tcgggccgga cggggtggtg
      541 gagtgggtcc acgaaaccga cactccgctc gatgtccccg gcccgaacct catcctcgaa
      601 gccatccgcg cctagatccc ggtccggaat atttccgggc cggctttgat gaccagggca
      661 tcgatttcgc tcaggtccca ggccgtcgag agggttgccg ggtaagactc gccttcgtcg
      721 acgtcatagc ggtagcggaa ggttccggcc ggctccagtt tgccattggc cgtcttcacg
      781 aagacccggc aaagggtgcc cggcttcatc ccgtccacct tgacctcgat accggtgccg
      841 gtcgccttcg gttccaggtt gcccgcgatc gacaccccgg ggggcagcga ggtgaaggcg
      901 aactgctggc cggcgctcgc cgttccatcg ccgccgttgc caccgcccag cgcaagcaca
      961 agggctatcc caaccgctgc gagtgcccct atggccggaa gtgcgatgcg ccagggtcgc
     1021 cgaccggtct tcggctgcgc tacgggctcg tcgtcccgca taccggccat gacccggtcc
     1081 tcgagcccgg gggagggttg aggcaacggt tcggtaaggg tgtcgaccac agagggat
//
LOCUS       DAOTIP010000728         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_152185_length_1137_cov_2.576710, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000728 DAOTIP010000000
VERSION     DAOTIP010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152185_length_1137_cov_2.576710"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="P5138_11545"
     CDS             complement(<1..489)
                     /locus_tag="P5138_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV61258.1"
                     /translation="MSSGRKAVVVGAGLAGLTAAFELDRCGWDVTVHEARDRVGGRTW
                     TENLPNGVPIEMGAEFVLPGNTELVALAAELGIGTLDKGMRYGNREPRGGIGVQQEDL
                     AVGVAELRRTLEGIEASELSEPSAAELIASLEIDEGAREAILARCEISSAADASEVPA
                     GEL"
     gene            complement(486..>1137)
                     /locus_tag="P5138_11550"
     CDS             complement(486..>1137)
                     /locus_tag="P5138_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRV61259.1"
                     /translation="GRLQALLSGESVTLENGTEAAIEWLAAAGAPKVPVVVAATGPKV
                     ISVGARHAEMVDFTVGAEPTRLEWAIDHARQAAGGHDVSLGAFLDIGVSEDLATARNL
                     IRGSTAILARFGVESTSTEGLSDQTRAGIEQLAAEYEEAGHGKSKSVAAQGLSDEFID
                     RFGVCGPPDQVVERLKQLAALGLDRVVMVPGSLDADPDAVTESNRLFAEQVLPRLR"
BASE COUNT          166 a          432 c          352 g          187 t
ORIGIN      
        1 gagctcaccc gcgggcactt ccgaggcgtc cgccgccgag gagatctcgc agcgggcgag
       61 aatcgcttcg cgggcgccct cgtcgatctc cagcgaagcg attagctccg ccgcgctcgg
      121 ttccgacaat tccgacgcct cgatcccctc aagcgttctc cgcagctcgg ctaccccgac
      181 cgccaggtct tcctgctgaa ccccgattcc gcctcgcggt tcccggttgc cgtaccgcat
      241 gcccttgtcg agggtcccga ttccgagctc ggccgccagg gcaaccagtt cggtgttgcc
      301 gggcaggacg aactcggccc ccatctcgat cggcaccccg ttcgggaggt tctccgtcca
      361 ggtccgcccg ccgacccggt cccgcgcctc atgcacggtc acgtcccatc cgcatcggtc
      421 aagctcgaac gcggccgtga ggccggccag gccggccccg accacgaccg ctttccggcc
      481 ggagctcatc tcagacgggg caggacctgc tcggcaaaga gccggttgga ttcggtgacc
      541 gcgtccgggt cggcatcgag cgagccgggc accatcacga cccggtccag cccgagcgcg
      601 gcaagctgct tcaacctctc gaccacctgg tcaggcggtc cgcagacccc gaagcggtcg
      661 atgaactcgt cgctcaatcc ctgggcggcg accgacttcg acttgccgtg tccggcctcc
      721 tcgtactcgg ctgcgagctg ctcaatcccg gccctggtct ggtcggagag tccttcggtc
      781 gaggtcgatt cgaccccgaa ccgggcgagg atcgcggtac tgccccggat caggttccgg
      841 gcggtcgcca agtcttccga gaccccgatg tcgaggaatg cgccgagcga gacatcgtgg
      901 ccgcccgcag cctgccgcgc gtgatcgatg gcccactcga ggcgcgtcgg ctcagcccct
      961 accgtgaagt cgaccatctc cgcatgtctg gccccgaccg agatcacctt cggcccagtg
     1021 gcggcgacca ccacgggaac tttcggggca ccggcagcgg cgagccattc gatcgctgct
     1081 tccgtcccgt tttcaagcgt cacgctctcg ccggagagca gggcctggag ccgtccc
//
LOCUS       DAOTIP010000729         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_152548_length_1135_cov_3.197222, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000729 DAOTIP010000000
VERSION     DAOTIP010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152548_length_1135_cov_3.197222"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /locus_tag="P5138_11555"
     CDS             complement(<1..>1135)
                     /locus_tag="P5138_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV61260.1"
                     /translation="ADRLPEPLPSALLTSRRLPLLADAFREAHFPTGEEGTAIALRRL
                     AYQELLENQARMRGRRKRHREGAGPAIPLDRASRLAADWRSGLPFSLTGDQSHAIGEI
                     GAELAGDEPMGRLLMGEVGSGKTVVAVNAMLEAVGSGAQAAMMAPTEVLASQHFATLS
                     GLLEGSGVVVALLTGSTSAEERKDLLERLAAGEIGILVGTHALLEDPVRFGRLGLAVI
                     DEEHRFGVRQRAGLDGKAPPGHSAHLLHMSATPIPRTLALTAYGDLDVTTLREIPAGR
                     APVSTEVVSAAGRAEAFERLKAELDRGRQAFVVCPLVDESPMVEGRAAAAEAERLAGA
                     ELAGYEVGLLHGQMKPAQKEVAMQAFTDGRTDVLVATTVIEVGI"
BASE COUNT          173 a          418 c          361 g          183 t
ORIGIN      
        1 cgatccccac ctcgatcacc gtcgtggcca cgagcacgtc ggtcctgcca tcggtgaacg
       61 cctgcatcgc gacctccttc tgggccggct tcatctggcc gtggagcagg cctacctcat
      121 agccggcgag ttcggcaccg gcgaggcgtt ctgcctccgc ggcggccgcc cggccttcga
      181 ccatcggcga ctcgtcgacc agcgggcaga ccacgaacgc ctgccggccc cggtcgagtt
      241 ccgccttgag ccgctcgaag gcctccgccc tgcccgcggc cgaaaccact tcggtgctga
      301 ccggcgcccg gccggcagga atttccctca aagtggtcac gtcgaggtca ccgtacgcgg
      361 tcagggccag ggtgcggggt atcggggtgg ctgacatgtg cagaagatgg gcggaatgcc
      421 ccggcggagc cttgccgtcc aatccggctc tctgcctcac tccgaacctg tgctcctcgt
      481 cgatcaccgc cagaccgagc cgtccgaaac ggacgggatc ttcaaggagg gcatgtgtgc
      541 cgaccaggat cccgatttca ccggccgcaa gtctctccag cagatccttt ctctcctcgg
      601 ccgatgtcga gccggtaagc aacgcgacaa ccacgccgct tccctcaagc agaccgctga
      661 gagtggcgaa atgctgggag gccagcacct cggtcggggc catcatcgcc gcctgcgccc
      721 cgctgccgac cgcttcgagc atcgcgttta ccgcgaccac ggtcttgccc gaaccgacct
      781 ctcccatcag cagccggccc atcggttcgt cgccggccag ttccgccccg atctccccaa
      841 tcgcatgcga ctggtctccg gtcagtgaga acgggagccc tgaacgccag tccgcagcga
      901 gcctggaggc tcgatcgagg gggatcgccg gccccgctcc ttcccggtgc cgcttccgcc
      961 ggccgcgcat cctggcctgg ttctccagca actcctggta ggcgagccgc ctgagggcga
     1021 tcgcggttcc ttcctcgccg gtcgggaagt gagcctcgcg gaatgcgtcc gccaggagcg
     1081 gaaggcgccg gctggtgagc aaggcggaag gaagcggctc cggcaggcgg tcggc
//
LOCUS       DAOTIP010000730         1126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_154377_length_1126_cov_2.673203, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000730 DAOTIP010000000
VERSION     DAOTIP010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154377_length_1126_cov_2.673203"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="P5138_11560"
     CDS             <1..293
                     /locus_tag="P5138_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV61261.1"
                     /translation="GGSLVTGGATNSGGFARLSDEVVVAQNPDIIIVVPHGNSNDLSQ
                     VADYILNNEAWKTTNAVKNKKVYVSVNNELLQAGTDVGPTIGMIRSKWLKNW"
     gene            374..>1126
                     /locus_tag="P5138_11565"
     CDS             374..>1126
                     /locus_tag="P5138_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV61262.1"
                     /translation="MLLALVAATCASLAFGPVHIPLGEVIDTLLGRQVPETSKQIIEV
                     LRLPRTVEAIVVGAALGVAGALLQGALNNPLASPDVIGVTGGAGFGAMLVILLSPASV
                     ALLPLSALAFGLLAATLVFLIGFTGRGGGNITRIILAGIAIAALFSAATAGLMSAYPD
                     RVPNAIFFLVGGLTSTGWDNLQTIWPYFAVGFVIAAFMIRPLDKLMLGDDVAASLGSR
                     PRLIRLTAAFGAAILASAAAAIAGLLAFLGLLI"
BASE COUNT          168 a          396 c          355 g          207 t
ORIGIN      
        1 ccggtggcag cctggtcacc ggcggggcca cgaacagcgg cggtttcgcc cgcctctcgg
       61 atgaagttgt ggtcgcccag aatccggaca tcatcatcgt cgtgccgcat ggcaacagca
      121 atgacctcag ccaggtggcg gactacatcc tcaataacga ggcctggaag accaccaatg
      181 ccgtcaagaa caagaaggtc tacgtctcgg tcaacaacga attgctccag gccggtaccg
      241 acgtcggccc gacaatcggc atgattcggt ccaagtggct gaagaactgg tgaccgatcg
      301 ggccttcggg tcctgactcc gagcccgggg gaatgagctg acttctgtca ggccgatagc
      361 tgtcgccgcc gggatgctcc tcgccctggt cgcggcaacc tgcgcctcgc tcgccttcgg
      421 cccggtccac atcccgctcg gcgaggtgat cgacaccctg ctcggccggc aggtcccgga
      481 gaccagcaaa cagatcatcg aggtgctgcg gctgccccgg acggtcgagg cgatcgtggt
      541 cggagccgcg ctcggcgtgg ccggcgccct gcttcaggga gcactcaaca acccgctcgc
      601 ctcgccggac gtgatcggcg tgaccggcgg agccggtttc ggggcgatgc tggtgatcct
      661 gctcagcccg gcaagcgtgg cgctgctgcc gctaagtgcc ctggccttcg ggctgctcgc
      721 ggcaaccctt gtcttcctga tcggcttcac cggccggggc gggggcaaca taacccggat
      781 catcctcgcc ggcatcgcga tcgccgcact gttctccgcc gcgaccgccg ggctgatgtc
      841 ggcctatccg gaccgggtac cgaacgcgat cttcttcctg gtcggcggtc tcacctcgac
      901 cggctgggac aacctgcaga cgatctggcc ctacttcgcg gtcggcttcg tgatcgccgc
      961 tttcatgatt cgccccctcg acaagctgat gctcggcgat gacgtcgccg cttcgctggg
     1021 ttcccggccc cggctgatcc gtctcaccgc cgccttcggt gccgcgatac tcgcgtcggc
     1081 tgccgcggca atcgccgggc tgctcgcctt cctgggtctg ctcatt
//
LOCUS       DAOTIP010000731         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_154820_length_1124_cov_2.749298, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000731 DAOTIP010000000
VERSION     DAOTIP010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154820_length_1124_cov_2.749298"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="P5138_11570"
     CDS             complement(<1..959)
                     /locus_tag="P5138_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV61263.1"
                     /translation="MLRELRIENLLLIERAELRLGPGLNVLTGETGAGKTVLAHSLDL
                     LMGGKARKGIVRVGAPEAWVEGVFDLPPEWNEDEELSELLDRIPAGSDEVVLGRRVSE
                     SGRTSAFVGGRSATAADLQLLAGRLIAFYGQHEHRRLTIGSAQLAMVDGAAGLEQQQR
                     LADFREVWNRHRRLTGELEELLGRDMARERDLDLHRFELEEIDAANLTTGEKDAMGEE
                     RERLRHAEGLRESAAGAAVRLRGGEEADGAAALLAGALESLSGLRGIDPGLDELAGRV
                     EGLALEVEDVGVELGRYLDAIEADPERLAALEERLDLIDRLER"
BASE COUNT          190 a          408 c          319 g          207 t
ORIGIN      
        1 tttctttcca gtcggtcgat cagatcgagc cgttcttcca gggccgccag ccgctccggg
       61 tcggcttcga ttgcgtcgag gtagcgaccg agttccacac ccacatcctc aacctcgaga
      121 gcgagtcctt cgaccctgcc ggccagttcg tcgagtccgg ggtcgatgcc ccgcagcccg
      181 gaaagcgatt ccagggcccc ggccaggagg gcagcggcac cgtcggcttc ctctccgccc
      241 cggagacgaa ctgcggcccc ggccgccgat tccctcaggc cttccgcatg tctcaggcgt
      301 tcccgctctt cccccatcgc gtccttctcg cccgtcgtca gatttgccgc gtcgatctct
      361 tccagttcga agcgatgcag gtccagatct ctttccctgg ccatgtccct gccaagcagc
      421 tcctcgagct caccggtgag ccgccgatgg cggttccaca cctccctgaa atccgcaagt
      481 cgttgctgtt gctcgagccc ggcggcaccg tcgaccatcg ccagctgggc ggagccgatg
      541 gtcagccggc gatgctcgtg ctgaccgtag aaggcaatca acctgccggc cagcaactgg
      601 agatcggccg cggtggcgct ccttccaccg acaaaagccg aggtccggcc ggattcggag
      661 acccgtcgcc ccagcaccac ttcatcgctt ccggcgggga tccggtcaag cagctccgag
      721 agctcctcgt cctcattcca ctccggaggc agatcgaaaa ccccttcgac ccaggcctcg
      781 ggagcaccga cccgcacgat ccccttccgg gctttgccac ccatcagaag gtcgagcgaa
      841 tgggccagaa cagtcttgcc tgcacccgtt tcgccggtaa ggacgttcag cccgggaccg
      901 agcctaagtt cagccctctc gatcagcaga aggttttcga ttcgaagttc gcgcagcatc
      961 tgcgcaaact tctaacgccc ccgtcggacg aacaaatgtt cgcccacaag cggcgccctg
     1021 ctagaccgcg aggtgcccga acttctccct gatgcggtgg tagaagttcg agcccggcag
     1081 ctgggccaga ctggcgaggt cgggcaggta tcggacttcc gctt
//
LOCUS       DAOTIP010000732         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_154998_length_1123_cov_3.235019, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000732 DAOTIP010000000
VERSION     DAOTIP010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154998_length_1123_cov_3.235019"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="P5138_11575"
     CDS             complement(<1..249)
                     /locus_tag="P5138_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV61264.1"
                     /translation="MTFTPLTRNLLRVAVAITLAFIYIPLLIILLYSMNPEQTLSWPP
                     ESLTLEWFPKAIQDQAARDALWNSVKVGAAATSIALVLG"
     gene            complement(253..>1123)
                     /locus_tag="P5138_11580"
     CDS             complement(253..>1123)
                     /locus_tag="P5138_11580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV61265.1"
                     /translation="PPRRSVGRRLADLFQGHPRLTVAALLAAPIGWLVIGYLGSLAVM
                     LAASFWHLNELSGEVTQGFSLDNFDTIFSDEVYRTITFRTIGVAAAVTVTDAILAFPI
                     AFYMAKLAKPRMQSVLVVAVLLPLWSSYLVKVGAWRLMLSEDGAINWALSPFGLHGPG
                     YGTVAVWLVMSYIWLPYMIIPIYAGLQRIPDSLLSASEDLGAKQAKTFRKVIMPLTFP
                     AIVAGSIFTFSLTLGDYITPANVGNKQFIGNVVYSNFVANLPLAAAITVIPIVVMVIY
                     LLIARRFGAFEHV"
BASE COUNT          217 a          330 c          399 g          177 t
ORIGIN      
        1 cccgagcacc agggcgatcg aggtcgccgc tgctccgacc ttgaccgagt tccagagggc
       61 gtcacgggcg gcctggtcct ggatcgcctt cgggaaccat tcgagggtga ggctctccgg
      121 cggccaggaa agcgtctgct ccgggttcat cgagtagagc aggatgatca gcagcgggat
      181 gtagatgaag gccagggtta tcgcgacggc gaccctgagc aggttccttg tcagcggggt
      241 gaaggtcatc gctcagacgt gctcgaaggc tccgaaccgg cgggcgatca ggaggtagat
      301 gaccatcacc acgatcggga tcacggtgat cgcggcggcg aggggaaggt tggcgacgaa
      361 gttgctgtag acgacgttgc cgatgaactg cttgttgccg acgttggcgg gggtgatgta
      421 gtccccgagg gtcagcgaga aggtgaagat cgagccggcc acgatcgccg ggaaggtgag
      481 cggcatgatc accttgcgga aggtctttgc ctgcttcgcc ccgaggtcct cggaggcact
      541 caggagcgaa tcggggatgc gctggaggcc ggcgtagatc gggatgatca tgtacggcag
      601 ccagatgtag ctcatgacca gccagacggc gaccgtgccg tagccgggtc catgcaggcc
      661 gaacgggctc agggcccagt tgattgcgcc gtcctcggaa agcatcagcc gccaggcacc
      721 gaccttgacc aggtagctcg accagagcgg cagcagcacc gccaccacca gaaccgactg
      781 catccggggc ttggccagtt tcgccatgta gaaggcgatc gggaaggcga ggatcgcgtc
      841 ggtgacggtc accgccgcgg ccaccccgat cgtgcggaag gtgatcgtgc ggtagacctc
      901 gtcactgaag atcgtgtcga agttgtcgag gctgaatccc tgcgttacct cgccgctcag
      961 ctcgttcaga tgccagaagg aggcggcgag catcaccgcc agcgagccca ggtagccgat
     1021 caccagccat ccgatgggcg cggcaagaag cgccgcgacg gtcagtcgcg gatgcccctg
     1081 gaagaggtcg gcgagcctcc tgcccaccga cctccggggc gga
//
LOCUS       DAOTIP010000733         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_155338_length_1122_cov_2.052484, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000733 DAOTIP010000000
VERSION     DAOTIP010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155338_length_1122_cov_2.052484"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="P5138_11585"
     CDS             <1..425
                     /locus_tag="P5138_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRV61266.1"
                     /translation="DLIRDFRADRRGLSFDFSVRPVTEGLEGLDVAFYSWEGEPGPET
                     DALLESALQGGAKIAVAMCGRHGSRARGTGESVSVAGNEIQAVDTCGAGDSFIAHFVI
                     AHLEGSSLEAAMQAGTKAGEEMCRNLGAWLNHRPEKVA"
     gene            422..>1122
                     /locus_tag="P5138_11590"
     CDS             422..>1122
                     /locus_tag="P5138_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV61267.1"
                     /translation="MSTAAGPLVEIKGLKKTFGHVEALRGVDLEIAEGEVHALVGDNG
                     AGKSTVVKTLSGVITPDEGELRVRGQRYEFGNPGAAQAVGIETVFQDLALADSLPAGE
                     NVFLGREPLRKGFTRRLGFVDRNEMRVRTRKYLTELGVTLPSLNEPVGSLSGGQRQAV
                     AVARAAVWGSNMIIMDEPTAALGVRQTEFVLELITRMRDEKKLSILLISHNMPDVLRV
                     ADRISVLRLGQRVAT"
BASE COUNT          206 a          337 c          382 g          197 t
ORIGIN      
        1 cagatctgat ccgggacttc cgggcggatc gtcgtggact cagcttcgac ttttccgtcc
       61 ggccggtgac cgaaggcctc gaaggcctgg acgttgcgtt ctattcctgg gaaggtgaac
      121 cgggtccgga aaccgatgcc cttcttgaga gcgctctcca aggaggagcg aagatcgcgg
      181 tggctatgtg tggtcgccac gggagcaggg ctcgtggcac cggcgagtcg gtttccgtgg
      241 ctggcaacga gatccaggcg gtcgacacct gcggggccgg tgactcgttc atcgcgcatt
      301 tcgtcatcgc gcacctcgaa gggtcaagtc tcgaagcagc gatgcaggcg ggtaccaaag
      361 ccggcgagga aatgtgccgg aacctcgggg cctggctgaa ccatcgaccg gagaaagtgg
      421 catgagcacc gcagccgggc cactggtcga gatcaagggt ctgaagaaga cgttcgggca
      481 cgtcgaggcg ctccgcggag tcgatctcga gatcgccgaa ggcgaggtgc acgccctggt
      541 cggagacaac ggcgccggca agtcgaccgt agtcaagact ctttccggcg tcatcactcc
      601 cgacgaaggc gaactccggg tgcgcggaca gcgctacgaa ttcggcaacc ccggcgcggc
      661 ccaggcagtt ggcatcgaaa ccgtcttcca ggatctggcg ctggccgatt ctttgccggc
      721 cggggagaat gttttcctcg ggcgggagcc gctcaggaag ggatttaccc ggcggctcgg
      781 cttcgtcgat cgcaacgaga tgagggtacg tacccgcaag tacctgaccg aactcggcgt
      841 caccctgcct tcgctgaacg aaccggtggg atcgctctcc gggggccagc gccaggcggt
      901 agccgtggcc agggcggcgg tgtggggttc gaacatgatc atcatggacg agccgaccgc
      961 tgccctcggc gtgaggcaga ccgagttcgt gctcgaactg attacccgaa tgcgggacga
     1021 gaagaagctc tcgattctcc tgatctccca caacatgccc gacgtccttc gggtggccga
     1081 tcgaatctcg gtgctgcgcc tcggtcagag ggtcgccacc tt
//
LOCUS       DAOTIP010000734         1120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_155679_length_1120_cov_3.041315, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000734 DAOTIP010000000
VERSION     DAOTIP010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155679_length_1120_cov_3.041315"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="P5138_11595"
     CDS             complement(<1..611)
                     /locus_tag="P5138_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61268.1"
                     /translation="MSIREVKARTIQSIAIAAGRSDDPYVLDASENFVRSVNESQIDE
                     VLKQVGGGDGSELKRKDGRIKFGAAHSSSALAANAFGAWVDQTEGLNVAGIDGFSEIG
                     FEEKFPTGLNGKAPNLDVWLPSNENPVAIESKFTEYLESKKADFKDSYEGLVGEVAHQ
                     SWASVYRDLKLNPRRYERIDVAQLVKHYLGLRKAIQGGRFGAVT"
     gene            744..989
                     /locus_tag="P5138_11600"
     CDS             744..989
                     /locus_tag="P5138_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61269.1"
                     /translation="MVPIEEYVPPFALMIVGLLIGEAFGDASAIGAIVGGLFVWSFFW
                     TIRIWCEHGEAVRLLRERDRIERMARMLEELDRDRRA"
BASE COUNT          220 a          336 c          300 g          264 t
ORIGIN      
        1 gtcacggcac cgaaccttcc accctgaata gccttccgga gccccagata gtgcttgacg
       61 agctgagcga catcgatgcg ctcataccgc cgtgggttca acttcaggtc ccggtagacc
      121 gaggcccaac tctggtgcgc gacctccccg acaagccctt catatgaatc cttgaaatcg
      181 gcctttttcg attccagata ctcggtgaac ttcgactcga tcgccacggg gttctcgttg
      241 gaaggaagcc agacatcgag attcggcgcc ttgccgttga gtccagtggg gaacttctct
      301 tcaaagccaa tctcagagaa accgtcaatc cccgctacgt tcaagccctc cgtctggtcg
      361 acccaggctc cgaacgcgtt ggcggctaga gcagacgacg agtgcgcagc cccaaacttg
      421 attcgcccat ccttccgctt cagctcactt ccatcaccgc cgccgacctg tttcagaacc
      481 tcgtctatct gcgactcatt cactgaccgg acgaaattct cacttgcatc aagaacgtag
      541 ggatcatcac ttcgtccggc tgcgattgct atcgactgaa tagttctcgc cttcacctcc
      601 ctgatactca cggtcgatcc actctctcca agtcattccc tccttgtttg ttcatacgca
      661 atccgagtct aggttccggt cgttcttctg acaaccgggt tcgtttcgtc agagaccgcc
      721 gccggcttcc gacctacccg tacttggtcc cgatcgagga atatgtcccg ccctttgccc
      781 tgatgatcgt cgggctgctg attggtgaag ccttcggtga tgcgtcagca atcggggcga
      841 tcgtcggcgg attgttcgtc tggagtttct tctggaccat ccggatctgg tgtgagcacg
      901 gagaggccgt tcggttgctg cgggagcggg ataggatcga acggatggcg cggatgcttg
      961 aggagcttga tcgtgaccgg cgggcctgac ccagaggagg acccggcaga acccggagtc
     1021 aaagatgagc ttcaggacct ggttggttcg gttcggcgtc gtcgcgatga cgacgatgat
     1081 gatcaaaatc agcagcagtt atttgtgacg atgttatcaa
//
LOCUS       DAOTIP010000735         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_157199_length_1113_cov_2.815690, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000735 DAOTIP010000000
VERSION     DAOTIP010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157199_length_1113_cov_2.815690"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="P5138_11605"
     CDS             <1..671
                     /locus_tag="P5138_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV61270.1"
                     /translation="TSIAPPETEDYVVGGVGLGAIPKQQRGIDVRNRLYEAAINEIDA
                     KGIEESRVENIVAEAGTSWGTFFRYFPRKEDVLLYETARLFREEMRPAFDQAIADQDT
                     PVRESAREVILMIMEPRISPRFHAEMLAETLRHPARFAAILGEGELPLAVLMAGMLAE
                     GQSRGEVKTEAPIPVCAAVLLAGVMFSASTVLQAVADGTRPGSDIAEVANQAFDLAWS
                     GVGV"
     gene            747..830
                     /locus_tag="P5138_11610"
     tRNA            747..830
                     /locus_tag="P5138_11610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:781..783,aa:Leu,seq:tag)
     gene            886..>1113
                     /locus_tag="P5138_11615"
     CDS             886..>1113
                     /locus_tag="P5138_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61271.1"
                     /translation="MCRLFALHAGQNDVAVDFWLLEAPDSLAKQSEINADGFGIASLT
                     TDKELMLIKKPVEAATDATFRRAAKHLEAAEM"
BASE COUNT          210 a          360 c          364 g          179 t
ORIGIN      
        1 caacaagcat cgcaccaccc gaaaccgagg actatgtcgt cggcggggtc ggtctgggcg
       61 cgatcccaaa acaacagcgc gggatcgacg tgcggaaccg cctctacgag gcggcaatca
      121 acgaaatcga cgccaagggc atcgaggaat cccgggtcga gaacatcgtc gccgaggccg
      181 gcaccagctg gggcaccttc ttccggtact tcccgcgcaa ggaagacgtt ctcctttatg
      241 agaccgcccg gctcttccgg gaggagatgc gaccggcttt cgaccaggcg atcgccgacc
      301 aggacacccc ggtccgggaa agcgcccgcg aggtaatcct gatgatcatg gagccacgga
      361 tcagtccgcg gttccatgcc gagatgctgg ccgaaacgct ccggcatccg gcccgcttcg
      421 cagcgatact cggcgaaggg gagctgccgc tggcggtcct catggccggc atgctggccg
      481 aagggcaaag tcgcggcgaa gtcaagaccg aggctccgat cccggtttgc gcggccgtgc
      541 tcctggccgg cgtcatgttc tccgcatcga cggttcttca ggccgtcgcc gacggaaccc
      601 gccccggttc cgacattgcc gaagtggcaa accaggcctt tgatctcgcc tggtccggcg
      661 tcggagtctg accagcccgg gcgataagtc ggcacccgga gcgccgaggt gaaaaaatcc
      721 ccgggctgcc ctcgggcggt tcacaagcgg acgtggcgga actggtagac gcgtcaggtt
      781 taggtcctgg tgcccgcgag ggtgtggagg ttcgagtcct ctcgtccgca tcgaatcacc
      841 cggtcggtat gagggcggaa gcaggcggcc tcgtatgctc gtcgcatgtg ccgccttttc
      901 gcccttcacg ccggacagaa cgacgtggca gtcgatttct ggctgcttga agctcccgac
      961 tccctggcca aacagagcga gatcaacgcc gacggcttcg gcatcgcctc gctgacgacg
     1021 gacaaggagc tgatgctgat caagaagccg gtcgaggcgg ccaccgatgc caccttccgc
     1081 agggccgcga agcatctcga ggcagcggag atg
//
LOCUS       DAOTIP010000736         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_157594_length_1111_cov_3.390152, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000736 DAOTIP010000000
VERSION     DAOTIP010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157594_length_1111_cov_3.390152"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="P5138_11620"
     CDS             <1..>1111
                     /locus_tag="P5138_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV61272.1"
                     /translation="HGVPLTVRGGGTGLAGGAVPDGGVVLSLERLDRILSIDPELWRM
                     NVESGVRTATVHRIALENGLMFPPDPGASEQSTIGGNIATNAGGPHAFRYGVTGSWVT
                     GIEAVVPPGEILTTGGPLRKNVASLDLADLMIGSEGTLGIVTSAWLRLVPAPEERGVV
                     VGFYADTRTGCDAIGMIMGSGIQPTALEFLDRGALESSLGSFPSAAPDEAGFAVIAEA
                     DGTAEEVSGAVANLKEAMAEGALLPPATITDHVEIRNFWAWRDGVSIAVTAQRGGKIS
                     EDVVVPVESLAEAVEETIEIGRRHDLPACSWGHAGDGNLHSSFLVDLDNADQMRRGGV
                     AAAEIFEMAIRLGGSISGEHGIGSLKTGHVEAALGP"
BASE COUNT          185 a          359 c          396 g          171 t
ORIGIN      
        1 cacggtgtgc ccctgaccgt ccggggcggc gggaccggac tcgccggcgg cgcggtgccc
       61 gacggaggag tagtcctttc gctcgagcgg ctcgaccgga tcctctcgat cgaccccgag
      121 ctctggcgca tgaacgtgga atcgggagtg cggaccgcga ccgttcaccg gatcgccctg
      181 gagaacggcc tgatgttccc gcccgacccg ggagccagcg agcagtcgac catcggtggc
      241 aacatcgcca ccaatgcggg cggcccccac gccttccggt acggggtgac cggttcctgg
      301 gtcaccggaa tcgaagcggt cgtaccaccg ggcgagatcc tgactaccgg cggcccgctg
      361 agaaagaacg ttgccagtct cgacctggcc gacctgatga tcggctccga aggcactctc
      421 ggaatcgtga cctcggcctg gctgcggctc gttccggccc cggaggagcg cggggtcgtg
      481 gtgggtttct acgccgacac ccgaacgggc tgcgatgcga tcgggatgat catgggatcc
      541 gggatccagc ccaccgcact cgagttcctc gaccggggtg cgcttgagtc gagcctcggc
      601 tccttcccct cggcagctcc cgacgaagcg ggcttcgccg tgatcgcgga ggccgacggg
      661 acggcggagg aggtgtcggg cgcggtcgcg aacctgaaag aagcgatggc ggaaggcgcc
      721 ctgctccctc ccgcgacgat caccgaccac gtcgagatcc ggaacttctg ggcctggcgc
      781 gacggggtct cgatcgccgt gaccgcccag cgaggcggaa agatttccga ggatgtcgtg
      841 gtgccggtcg aatcgttggc cgaagcggtc gaggaaacga tcgagatcgg gcgacgccac
      901 gatctgccgg cttgctcatg gggacatgcc ggagacggca acctccattc cagcttcctg
      961 gtcgacctcg ataacgccga tcagatgaga aggggaggcg tggcggcggc ggagatattc
     1021 gagatggcga ttcgcctcgg cggttcgatc agcggcgagc acgggatcgg ctcgctcaag
     1081 accgggcatg tcgaagccgc gctcggaccg g
//
LOCUS       DAOTIP010000737         1108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_158199_length_1108_cov_3.266857, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000737 DAOTIP010000000
VERSION     DAOTIP010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158199_length_1108_cov_3.266857"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="P5138_11625"
     CDS             <1..438
                     /locus_tag="P5138_11625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HRV61273.1"
                     /translation="ATIRFITDGDVQAALLAVSEDTGIDLLWGIGGTPEGVLAASAIK
                     CLGGEIIGKLWPRNDEERQAAIDAGYDLDRVLTSNDLVSGDDVFFAATGVTDGDLLDG
                     VRRHKGWATTESLVMRSRSGTVRKVGARHDRAKLREVTGGLYG"
     gene            442..819
                     /locus_tag="P5138_11630"
     CDS             442..819
                     /locus_tag="P5138_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRV61274.1"
                     /translation="MVAERFIAAFSEADFETMRELLAPDLIAWITDSTGQMDRVEGRD
                     GYLARIEQMDLPGAEFRVELTQPPVEIESDKVLLMVEVHAERGGRSLHNFAGHLLRLD
                     GEQIAEWWMVDAKPAESDQFWSE"
     gene            838..>1108
                     /locus_tag="P5138_11635"
     CDS             838..>1108
                     /locus_tag="P5138_11635"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRV61275.1"
                     /translation="MAGTTLVAGSTGYIGRLLAERLAEEDGQVRCLARDPGKAADLGR
                     RGCEVVAGDVLDPGSLEEPLSGVRVAYYLVHSMGRGASGDFAETDL"
BASE COUNT          183 a          350 c          387 g          188 t
ORIGIN      
        1 gcaacgatcc gcttcatcac cgacggggac gttcaggcgg ccctgctggc ggtgtccgag
       61 gacaccggaa tcgacctgct ctggggcatc ggcggcacgc cggaaggcgt tctggcggcc
      121 tcggcgatca agtgcctggg cggagagatc atcggcaagc tctggccccg caacgacgag
      181 gagcgtcagg ctgcgatcga cgccggctac gacctcgacc gggtcctgac ttcgaatgat
      241 ctcgtctcgg gagacgatgt cttcttcgcg gcgaccggtg tcaccgacgg tgacctgctc
      301 gacggtgtcc gccgccacaa gggctgggcc acaacggagt cgctcgtcat gcgctcgcgt
      361 tcagggaccg tccgcaaggt cggtgcccgc cacgaccgcg ccaagttgcg cgaagttacc
      421 ggcggcctct acggttgagc catggtcgcc gagcgattta tcgccgcgtt ctccgaagcc
      481 gacttcgaga cgatgcgcga actgctcgcg cccgacctga tcgcctggat caccgattcg
      541 accggccaga tggaccgggt ggaaggccgt gatggttatc tggcaaggat cgagcagatg
      601 gacctccccg gtgccgagtt ccgggtcgag ctcacccagc cgccggtcga gatcgaatcc
      661 gacaaggtgc ttctgatggt cgaggttcac gccgagcgcg gtgggcgcag cctccacaac
      721 ttcgccggcc acctgctgcg ccttgacggc gagcagatcg ccgaatggtg gatggtcgac
      781 gccaagcccg ccgaaagcga ccagttctgg tccgagtagt tgggcggagg tcaggccatg
      841 gccggaacga cactggtagc cggctccacc gggtacatcg gccgtctgct tgccgaaagg
      901 ctggcggaag aggacggtca ggtccggtgt ctggcccgcg atcccggtaa ggcggccgac
      961 ctcgggcgac ggggctgcga agtggtggca ggggatgtgc tggatcccgg atccctggag
     1021 gagccacttt ccggagtgcg tgtcgcctat tacctcgttc attcgatggg ccgcggggcg
     1081 agcggcgact ttgccgagac cgatctcg
//
LOCUS       DAOTIP010000738         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_158634_length_1106_cov_3.209324, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000738 DAOTIP010000000
VERSION     DAOTIP010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158634_length_1106_cov_3.209324"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="P5138_11640"
     CDS             <1..355
                     /locus_tag="P5138_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV61276.1"
                     /translation="AGVPHSADVAIDDEIFGPVFNLIPVDGQEQAIEVANSSSFGLMG
                     SVFSADPQRALAVAEVLESGGVVINGTDNYRPPVIPFGGVKLSGRGVEGIGYTLEEMT
                     REKPIIFRRFREPRG"
     gene            358..>1106
                     /locus_tag="P5138_11645"
     CDS             358..>1106
                     /locus_tag="P5138_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV61277.1"
                     /translation="MARQQGDGKYQVPAVVGAISVLNELSGTGEAGATLSQLVESTGL
                     SKSTMHNLLSTLDSHGFVRRDANTRQYRLGPALIPLGNEAARQVKLVRSTLDRVAPLA
                     GRYGLSFAVAQPIDSSELRIISRFYPPGDVHVGITVGSAYGPMDGALGKVLLSAMDED
                     QARRLIKGRKLPAHTRATITSPESLIGEVEEVRSRGWSVSHGEFNENNAVAAGIWGTG
                     GDLELVLLALGFPTQFDGERLAEIGEVLRDTA"
BASE COUNT          186 a          364 c          376 g          180 t
ORIGIN      
        1 ggccggggtg ccccattcgg ccgacgtggc catcgatgac gagatcttcg ggccggtctt
       61 caacctgatc cctgtcgacg gacaggagca ggcgatcgag gtcgccaact cctcctcctt
      121 cggcctgatg ggctcggtct tctcggccga cccgcagagg gcccttgctg tcgccgaggt
      181 cctcgagtcg ggaggcgtcg tgatcaacgg caccgacaac taccggccgc cggtgatccc
      241 cttcggcggg gtcaagcttt ccgggcgcgg cgtcgaggga atcggctaca cgctggagga
      301 gatgacccgg gagaagccga tcatcttccg gcgcttccgg gaaccgaggg gctagcgatg
      361 gcccggcagc agggcgacgg caagtaccag gtgccggctg tggtcggggc gatcagcgtg
      421 ctgaacgaac tttccggcac cggtgaggcg ggggcgaccc tttcacagct ggtcgaatca
      481 accgggctgt ccaagagcac catgcacaac ctgctctcga ccctcgacag ccacggcttc
      541 gtgcgccggg acgcaaacac gaggcagtac cggctcggtc cggccctcat tcccctcggc
      601 aatgaggcgg cccgtcaggt caagctggtc cgcagcaccc tcgaccgggt ggcccctctg
      661 gccggccgtt acggactctc cttcgcggtt gcccagccga tcgactcgag tgagctacgg
      721 atcatcagcc gtttctaccc gccgggtgac gtccacgtcg gcatcaccgt cggtagcgcc
      781 tacggaccca tggacggggc gctcggcaaa gtcctgctct ccgcgatgga cgaagatcag
      841 gcgagaaggc tgatcaaggg ccgcaagctt cctgcccaca cccgggcgac catcaccagt
      901 cccgaatccc tgatcgggga ggtggaggag gtgaggtcgc ggggctggtc cgtctcccac
      961 ggcgaattca acgagaacaa cgctgtggcg gcggggatct gggggaccgg cggtgacctc
     1021 gaactggttc tgctcgctct cggattcccg acccagttcg acggggagcg gctcgctgaa
     1081 atcggagagg tgctgcggga cacggc
//
LOCUS       DAOTIP010000739         1104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_159066_length_1104_cov_2.818875, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000739 DAOTIP010000000
VERSION     DAOTIP010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159066_length_1104_cov_2.818875"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..441)
                     /locus_tag="P5138_11650"
     tRNA            complement(369..441)
                     /locus_tag="P5138_11650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Thr,seq:cgt)
     gene            509..>1104
                     /locus_tag="P5138_11655"
     CDS             509..>1104
                     /locus_tag="P5138_11655"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRV61278.1"
                     /translation="MAEADEHADLPSLERGRDLSRIYAFTDGVFAIAITLLVLQIEVP
                     SGLTSNSQFLDALNDLVPDFFAFAISFLVIGSYWIRHHRLMRMVREYDQTLMAVTMFY
                     LAWVVLLPFSSQLIGEYGSEVTLTTVFYILNLALIGASLTLTIRVIVKHRLSDPKYEW
                     ELESGFKSSAFVSGIFLLTAPIGLVLGGWTPLLWIVLMR"
BASE COUNT          192 a          327 c          335 g          250 t
ORIGIN      
        1 gaagcctcaa gagcctgatt ttccgggtta agggcaagag gggtactttg gtcaaagctt
       61 catggttccc agcgagtgtg agtccctgag acgaactcac tgctcgatca cattttctct
      121 ccggtcaaag gtggtcgatc cgtagtcata aaggtctggc cgatctcgga cgtatgcact
      181 cggattgtcc ccctggtgca gggagggaca gagctacagg agtctcgttc ccgggcaggg
      241 gagtgaaact cactgctcgt tggtcccgca gggtccttga tgcttggcag ggagactgat
      301 gttccctcaa gttcccgatc ttgggggatt gaccccttca tcattgttcc cgatggctct
      361 gggagtgaag ccgacgaccg gagtcgaacc ggtgacccct tcattacgag tgaagtgctc
      421 taccaactga gctacgtcgg cgctggaggg gcattgtagg tggacttggc cggtcgggtt
      481 cgtccggtgc tggtggcagg atcggcctgt ggctgaagct gacgagcacg ccgaccttcc
      541 ctccctggaa cggggccggg atctttcgcg gatctatgcc ttcaccgacg gcgtcttcgc
      601 gatcgcgatc accctgctcg tgctgcagat agaggttccc tccggcctga cctcgaacag
      661 ccagttcctc gacgcgctga acgacctggt tcccgatttc ttcgccttcg cgatcagctt
      721 cctggtgatc ggcagctact ggatccggca ccaccggctg atgcgcatgg tccgggaata
      781 cgaccagacg ctgatggcgg tgaccatgtt ctacctggcc tgggtcgtgc tgcttccctt
      841 ctccagccag ctgatcggcg agtacggcag cgaggtcacc ctcaccaccg tcttctacat
      901 cctgaacctg gccctgatcg gcgcctcgct gaccctgacg atccgggtga tcgtcaagca
      961 ccggctgagc gatccgaagt acgagtggga actggaatcg ggattcaagt cctcggcgtt
     1021 cgtaagcggg atcttccttc tcaccgcacc aatcggactg gtgctgggcg gatggacacc
     1081 cttgctctgg atcgtcctga tgcg
//
LOCUS       DAOTIP010000740         1104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_159108_length_1104_cov_2.329838, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000740 DAOTIP010000000
VERSION     DAOTIP010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159108_length_1104_cov_2.329838"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="P5138_11660"
     CDS             <1..805
                     /locus_tag="P5138_11660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRV61279.1"
                     /translation="ICPDCMTTTPVGMRCPECATEKTRVRSGPAATSQVGGYPATIAL
                     IAINVVVFLAEMATGSGGLSSTSSDLISDFGLRGAQVADGETYRLVTSGFLHAGFMHI
                     AFNMIALYFLGRVLEPSIGTVRFLMVYFASLLAGSFGAVLLSDNLALTVGASGAVFGL
                     FAATFIIAYGRGLGHVANEIGLIIGINLLLTFTISGISIGGHLGGLIGGGLCGILVIA
                     GERGYLGSNARLLEYGGMIAIAAVSVFAAISAAEPVPGLHFAVLNLPFL"
     gene            complement(746..>1104)
                     /locus_tag="P5138_11665"
     CDS             complement(746..>1104)
                     /locus_tag="P5138_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263046610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61280.1"
                     /translation="EMIEIAGGRDLVGKAGLKSAEHGWDELANLDPDLTVVMPCGYYV
                     DEAATQADDYMDRIRSTGAERTVAVDAAASFSRPGPRLIDGIELLAGLLHPEVFPLEE
                     GQIQYREMQTGDRFGG"
BASE COUNT          169 a          363 c          339 g          233 t
ORIGIN      
        1 gatctgcccg gactgcatga cgacgacccc ggtcggcatg cgctgcccgg aatgtgcgac
       61 cgaaaagacc cgggtccgca gcggtcctgc ggcaacctcc caggtcggcg gctacccggc
      121 cacgatcgcc ctgatcgcga tcaacgtggt cgtattcctg gcggaaatgg cgaccggatc
      181 cggtggtctc agctcgacca gttcagatct gatcagcgat ttcggcctgc gcggcgcaca
      241 ggtggccgac ggcgagactt accggctggt caccagcggt tttctccacg ccggcttcat
      301 gcacatcgcc ttcaacatga tcgcccttta cttcctggga cgggtactcg agccttcgat
      361 cggcacggtg cgcttcctga tggtctactt cgcgtcgctt ctggccggtt ctttcggggc
      421 cgtcctgctc agcgacaacc tcgccctgac cgtcggtgcc tccggcgccg tcttcgggct
      481 cttcgcggcg actttcatca tcgcctacgg ccgtgggctc ggccatgtgg cgaacgagat
      541 cggattgatc atcggtatca acctgctcct caccttcacg atcagcggca tcagcatcgg
      601 cggccatctc ggtggtctga tcggcggcgg gttgtgcgga attctggtca tcgccggcga
      661 gcgtggttac ctgggttcga atgcccgcct gctcgagtac ggagggatga tcgcgatcgc
      721 cgcagtttcg gtattcgccg cgatctcagc cgccgaaccg gtccccggtc tgcatttcgc
      781 ggtactgaat ctgcccttcc tctagaggaa aaacctcggg atgcaacaga cccgccagca
      841 attcaattcc gtcgatgagg cgagggcctg gccttgagaa cgaggcggcg gcgtcaaccg
      901 cgacggtccg ctctgctccg gttgacctga tccggtccat gtagtcgtcg gcctgggtgg
      961 cggcctcatc gacgtagtag ccgcagggca tgaccacggt caggtccgga tcgaggttgg
     1021 ccagttcgtc ccatccatgt tcagctgact tcaggcctgc tttgccgacc aggtcgcggc
     1081 cgccggcgat ctcgatcatt tccg
//
LOCUS       DAOTIP010000741         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_159895_length_1100_cov_3.968421, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000741 DAOTIP010000000
VERSION     DAOTIP010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159895_length_1100_cov_3.968421"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..>1100)
                     /locus_tag="P5138_11670"
     CDS             complement(39..>1100)
                     /locus_tag="P5138_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61281.1"
                     /translation="YKWDPHDGSATDPYAGIATGDKAPPEGFTADDFKIPTFEEVLDA
                     FPDTPINIELKQVAGVDIQDTAETMAGILASHPGHDENVIINSFGQEMLDAMAAARPQ
                     HKSYSGSQSALLGYIQGQPITPTPVALEPPDYYKLSGNWLRTVPILKPFADYDGYKIY
                     VWGSDHDEAQDTDPFYAKLIEEGADSYNTPDPLNLARYLCENGIASADGEPRCPEQVC
                     PEGQTGIAPDNCVDLPAIKVKGLRLSPAKGKLKAGKKKVLTLSITGTNGYKGRATIRL
                     KSSNRNVKLKKSITVNLLSGKTLKKKITIRATRKARGKAKITASSGKFRSRSTLSIKS
                     VCSKKKSGGGQVCGKTNHL"
BASE COUNT          159 a          333 c          362 g          246 t
ORIGIN      
        1 ggcagaggaa cgcctccgcg gcgactgacc gcaggttctt acaggtggtt ggtcttgccg
       61 cagacctgac cgccgccgga tttcttcttc gagcaaacgc tcttgatact gagggtcgag
      121 cgcgaccgga acttgcccga gctggccgta atcttcgcct tgcccctggc cttcctggtc
      181 gccctgatgg tgatcttctt cttcagggtc ttgcccgaga gcaggttcac cgtgatcgat
      241 ttcttcaact tcacgttccg gttcgatgac ttgagccgga tagtcgcgcg gcccttgtag
      301 ccgttggtgc cggtgatcga gagggtgagg accttcttct ttccggcctt gagcttgccc
      361 ttcgccgggc tcaggcgcag acccttcact ttgatcgcag gcaggtcgac gcagttgtcc
      421 ggggcgatgc cggtctggcc ctccgggcag acctgctccg ggcagcgcgg ttctccgtcg
      481 gcgctggcga tgccgttctc gcacaggtac ctggccaggt tcagcgggtc gggcgtgttg
      541 taggagtcgg cgccttcttc gatcagcttg gcgtagaaag gatcagtgtc ctgggcctcg
      601 tcgtggtcgg atccccagac gtagatcttg tagccgtcgt agtcggcaaa cggcttgagg
      661 atcggtaccg tccgcagcca gttgccagag agcttgtagt agtcgggcgg ctccagcgcg
      721 accggggtcg gggtgatcgg ctggccctgg atgtagccga gcaacgcgga ctggctgccg
      781 ctgtaggact tgtgctgcgg acgggccgcg gccatcgcat ccagcatctc ctggccgaag
      841 gagttgatga tcacgttctc gtcgtgacca gggtggctgg ccagaatccc cgccatggtc
      901 tccgcggtgt cctggatgtc gaccccggcc acctgcttga gctcgatgtt gatcggcgtg
      961 tccgggaacg catcgaggac ttcctcgaag gtcgggatct tgaagtcatc cgcagtgaag
     1021 ccctccggcg gggccttgtc accggtcgcg attcccgcgt acggatcagt agccgagccg
     1081 tcatgcggat cccacttgta
//
LOCUS       DAOTIP010000742         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_160858_length_1096_cov_2.830932, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000742 DAOTIP010000000
VERSION     DAOTIP010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160858_length_1096_cov_2.830932"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /locus_tag="P5138_11675"
     CDS             <1..>1096
                     /locus_tag="P5138_11675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRV61282.1"
                     /translation="QGNSGKNDGNQSKNDSSGNSTGNQGKNNGNQTANPKNDITGAAA
                     DIIPGADSPLSAKITVDPSFGLSGDSTPVAACDGSAGPPRNLIPIYVEASKKYGLGER
                     GPQILAAINSIETDFGRLNEVTSSAGAIGWMQFMPSTWDMYATDGDGDGKTDPYNPHD
                     AIHAAARYLQAAGAPTDWYDAIYAYNHADWYVQDVLDKAACYGSLGDTVQTEEQLKNY
                     ICQASDARVLEIPNYYMRGFEDAASRYGLGKRGIWVLAAVARLESDYGRGMTPQQLKD
                     TGPMGLSREEWKKYGVDGDGDGYVERDEPWDGIATFARLLWSQPTLEEGLFEHNHATW
                     YVEAAVQDAGRIAGDCQAVKTDWNIAYPVPT"
BASE COUNT          237 a          384 c          332 g          143 t
ORIGIN      
        1 ccagggtaac tccggaaaga acgacgggaa ccagagcaag aacgacagct ccgggaactc
       61 gaccgggaac cagggaaaga acaacggcaa ccagactgcg aacccgaaga acgacatcac
      121 cggcgcggca gccgacatca tccccggcgc cgactcgccg ctctcggcca agatcacggt
      181 cgatccctcc ttcggccttt ccggtgattc gacaccggtg gcagcctgcg acggcagcgc
      241 cggaccgccc cgcaacctga tcccgatcta cgtcgaagcg tccaagaaat acgggctcgg
      301 tgaacgcggc ccgcagatcc tggccgcgat caactcgatc gaaaccgact tcggccgact
      361 gaacgaggtc acctcctcgg ccggggcgat cggctggatg cagttcatgc cctcgacctg
      421 ggacatgtac gcgaccgacg gtgacggtga cggcaagacc gacccgtaca acccgcacga
      481 cgcaatccac gccgctgccc gctacctgca ggccgccgga gccccgaccg actggtacga
      541 cgcgatctac gcctacaacc acgccgactg gtacgtccag gacgtgctcg acaaggccgc
      601 ctgctacggg tccctgggcg acacggtcca gaccgaggaa cagctcaaga actacatctg
      661 ccaggcctcc gacgcacgag tactcgagat ccccaactac tacatgcggg gcttcgagga
      721 tgccgcttcc cggtacgggc tcggcaagcg cggaatctgg gtcctcgccg ccgtggcccg
      781 gctcgaatcc gactacggac gcggcatgac cccgcaacag ctcaaggaca ccggaccgat
      841 gggcctctcc cgtgaagaat ggaagaagta cggggtcgat ggggacggcg acggttacgt
      901 cgagcgggac gagccctggg acggcatcgc caccttcgcc cgcctgctct ggtcgcagcc
      961 gaccctggaa gaaggactct tcgaacacaa tcacgccacc tggtacgtcg aagctgcggt
     1021 ccaggacgcc gggcggatcg ccggtgactg ccaggcggtg aagaccgact ggaacatcgc
     1081 ctatccagtg ccgacc
//
LOCUS       DAOTIP010000743         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_161273_length_1094_cov_3.118383, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000743 DAOTIP010000000
VERSION     DAOTIP010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161273_length_1094_cov_3.118383"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..>1094
                     /locus_tag="P5138_11680"
     CDS             177..>1094
                     /locus_tag="P5138_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRV61283.1"
                     /translation="MAEIGIIAAVLFAYSLVSDKLSGWAITPPMVFVAAGVLLGPDVL
                     GVFDLTLTGETGLALAEIALVIVLFADAARIDLRALSGNRNLPVRLLGIGMPLTIILG
                     VLIGAVLLKEVEFWEAAIVAAILAPTDAALGQAVVSSQKVPQRIRQALNVESGLNDGL
                     SIPFLFLFVGLAVTETVVTATGWATFVIEQIGIGAGVGLATGLAGGFLFERATDRDLV
                     STPFQQLGLVGLAVGSWALADFLGGNGFIAAFTAGLAAGRITPHTGQRILAFTEDEGQ
                     LLNLIIFFVFGASAITLLDGLTWHVIVYGL"
BASE COUNT          167 a          383 c          344 g          200 t
ORIGIN      
        1 cgcttcccca aacggccgct ggtcacggtc gagttcttcg aaccggcggg tggccagccg
       61 cgtccggacg aagacgacca ggaaatggcc cagcgtctgc tcgacgaaat ccgcgccaag
      121 gcgccgatcg cctcctgacc cggagccacc ggaccctcga ttagagtcgc tgccagatgg
      181 ccgaaatcgg aatcatcgcc gcggtcctgt tcgcctacag cctggtttcg gacaagctct
      241 ccggctgggc catcactcct cccatggtgt tcgttgcggc aggtgtcctg ctcggccccg
      301 acgtgctggg ggttttcgat ctgaccctga ccggcgagac cggactggcc ctcgccgaga
      361 tcgccctcgt gatcgtgctc tttgcggacg cggccagaat cgaccttcgc gcactgagcg
      421 ggaaccgcaa cctgccggtc cggctgctcg gcatcgggat gccgctgacc atcattctgg
      481 gcgtcctgat cggcgccgtg ctgctgaagg aagtcgagtt ctgggaagcc gcaatcgtgg
      541 cggcgatcct cgctcccacc gacgccgccc tgggccaggc cgtggtttca agccagaagg
      601 tgcctcagcg aatcaggcag gccctgaacg tggaaagcgg cctcaacgac ggactctcga
      661 ttccgttcct cttcctcttc gtgggactag cggtgaccga aaccgtcgtg accgccaccg
      721 gatgggcgac cttcgtgatc gagcagatcg ggatcggcgc gggggtcgga ctcgccaccg
      781 gactggccgg cggcttcctc ttcgagcggg ctacggaccg cgacctggtc tccacgccct
      841 tccagcagct cggcctggtc gggctggccg tcggatcctg ggccctggcc gacttccttg
      901 gcggaaacgg cttcatcgcg gccttcaccg ccgggctggc agccggacgc atcacccctc
      961 acaccggtca gaggatcctc gcgttcaccg aggacgaggg ccagctcctc aacctgatca
     1021 tcttcttcgt gttcggtgcc agcgcgatca cgctgctcga cggcctgacc tggcacgtga
     1081 tcgtctacgg cctg
//
LOCUS       DAOTIP010000744         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_162111_length_1091_cov_1.945946, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000744 DAOTIP010000000
VERSION     DAOTIP010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162111_length_1091_cov_1.945946"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="P5138_11685"
     CDS             <1..357
                     /locus_tag="P5138_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61284.1"
                     /translation="QAQQAIRNVSSAPESQGSTPKELAEWSVDTKNSGWGTQAEDATR
                     TIGEASPQVRESVITGGQGYGGGGNTQTPRPAPAQANGNGNGNGGGTPRPGSGTKAVP
                     DPTEGHKRPASKRGES"
     gene            358..750
                     /locus_tag="P5138_11690"
     CDS             358..750
                     /locus_tag="P5138_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61285.1"
                     /translation="MNPAYRHLEEPVKIGDFTLGQIAGFLVAAMLAMLWGFVISPLPP
                     IWSLFTAIYIGGLPAMAVLFASTTDFDAFRMVKAYVLFQRRPGRFVPGAGRLEDRGYV
                     VKKPPVEKHRPVNTKTDEILIGEALWGD"
     gene            783..>1091
                     /locus_tag="P5138_11695"
     CDS             783..>1091
                     /locus_tag="P5138_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61286.1"
                     /translation="MPEAGEIFEIDALDRDGLMIRSDGTFIRMLEVTPRNPELLSIEE
                     AQAMSATYAQMLARLRPKQSIQFIIDSRPVHVDRVLEASRKEVERVAGPAPTTPELRR
                     A"
BASE COUNT          249 a          342 c          342 g          158 t
ORIGIN      
        1 caggcacaac aggcaatccg gaacgtaagc agcgccccgg aaagccaggg ctcaacaccc
       61 aaggagcttg ccgaatggtc ggtcgacacg aagaactccg gctggggaac ccaggcagaa
      121 gatgcgaccc gaacgatcgg ggaggcatcc ccacaggtcc gtgaatcagt gatcaccggc
      181 gggcaaggct acgggggagg cggcaacacc caaacgccaa ggccggcacc cgcacaagcg
      241 aacggcaacg gcaacggcaa cggcggcgga accccgcggc cgggatccgg aaccaaagcc
      301 gtaccggacc cgaccgaagg acataaacga cctgcctcca agaggggaga aagctaaatg
      361 aacccggcct acaggcacct tgaggaaccg gtcaagatcg gtgacttcac actcggccag
      421 atcgcaggtt tcctggttgc ggcgatgctc gcaatgttgt ggggattcgt gatcagtccg
      481 ctcccgccga tctggtcgct gttcaccgcc atttacatcg gcgggcttcc ggcgatggcg
      541 gtcctgttcg cctcaaccac cgactttgac gcgttccgga tggtgaaggc ctatgtgctc
      601 ttccaacggc gtcccggccg gttcgtgcca ggtgccggcc gtcttgaaga ccgcggatat
      661 gtggtcaaga agccgccggt cgaaaagcat cgtccggtca acacgaaaac cgacgaaatc
      721 ctgattggag aagcactgtg gggggactag ccgaaaagat ccggccaacc gaggttgagg
      781 acatgcccga agcgggggag atcttcgaga tcgacgcgct cgaccgggac ggactgatga
      841 tccgctccga cggcacgttc atccggatgc tcgaggtgac ccctcgaaac ccggagctct
      901 tgtcgatcga ggaagcgcag gcaatgtcgg ccacctacgc gcaaatgctg gccaggctcc
      961 ggccgaagca aagcatccag ttcatcatcg actcccggcc cgttcatgtc gaccgggtac
     1021 ttgaagcgtc ccgcaaggaa gtcgaaaggg ttgccggtcc ggccccgaca acacccgagc
     1081 tccggcgcgc g
//
LOCUS       DAOTIP010000745         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_162471_length_1089_cov_3.317215, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000745 DAOTIP010000000
VERSION     DAOTIP010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162471_length_1089_cov_3.317215"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="P5138_11700"
     CDS             <1..684
                     /locus_tag="P5138_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61287.1"
                     /translation="DLQQGGDRTKWHFEIKNHADGRAQLKLFATCLGGKTVENSHQHW
                     IKIRPKKTTAWFPGGDFSSPALGCIPGKEVAVGPGFKLLSGGDVWEKSSYPGNPLSSS
                     WNWNFVVSSPSAIEVSVLCLRKQTGWKWHHRHNLKIWLKPGWWPNGARTLTKNSVQEL
                     RVSCYDGYRAMVGAFGVAPSYHGYIHFLGMDPRPKTRAFKFWNTDPFNDLPIYLATIC
                     IGNRTGHAY"
     gene            complement(772..>1089)
                     /locus_tag="P5138_11705"
     CDS             complement(772..>1089)
                     /locus_tag="P5138_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61288.1"
                     /translation="TFVTVIVASVIVTAALGVATYRTVQEANEISAATDVGRTWAKEN
                     GEKLVKVAYEGNDLVFLVEGSADGSKDRELSTMLEGSIPDGTSLVINRVPGERKQLPS
                     VGS"
BASE COUNT          203 a          342 c          335 g          209 t
ORIGIN      
        1 gacctccagc agggcgggga ccgtaccaag tggcatttcg agatcaagaa ccacgccgat
       61 ggccgggccc agctgaaact ctttgcgacc tgcctcggtg gcaagactgt cgagaacagc
      121 catcagcact ggatcaagat tcgtcccaaa aagacgactg cctggttccc cggaggtgac
      181 ttctcgagtc cggccctggg ctgcattccg ggcaaagaag ttgccgttgg tcctgggttc
      241 aagctgctct cgggtggcga cgtctgggag aagagctcct acccaggcaa tcccctgagc
      301 agttcctgga actggaactt cgtggtttcg tccccgtccg cgatcgaggt cagtgtgctc
      361 tgtctcagga aacagaccgg atggaaatgg catcaccggc acaacctgaa gatctggctg
      421 aagcccggat ggtggccgaa cggcgcccgt accctgacca agaactcggt tcaggaactg
      481 cgggtgtcct gctacgacgg ttacagggcg atggtcgggg cattcggggt cgccccgtcc
      541 taccacggat acatccattt cctcggcatg gatccgcggc ccaagacccg cgcattcaag
      601 ttctggaata ccgatccgtt caacgatctg ccgatctatc tggcgacgat ctgcataggt
      661 aaccgcacgg gccacgctta ctgagggcgt ccggcgaacc gggagagagg ccagggcgga
      721 gcccctaccg ccggcctctc ttcctcgaac cggagggcgc tgcggcagtt ctcagctgcc
      781 gaccgacggc agttgcttgc gctcgcccgg gacccggttg atgaccagcg aagtgccatc
      841 cggaatgctg ccctccagca ttgtggagag ttcccggtcc tttgagccgt cggcacttcc
      901 ctcgacgaga aatacgaggt cattgccttc gtaggcgacc ttgaccagtt tctcgccgtt
      961 ctccttggcc caggtccggc cgacgtcggt cgccgcggag atttcgttgg cctcctggac
     1021 ggtccggtag gtggccactc ccagggcggc ggtgacgatc acactggcca cgatgacggt
     1081 cacgaacgt
//
LOCUS       DAOTIP010000746         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_164314_length_1081_cov_3.009747, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000746 DAOTIP010000000
VERSION     DAOTIP010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164314_length_1081_cov_3.009747"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..238
                     /locus_tag="P5138_11710"
     CDS             65..238
                     /locus_tag="P5138_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61289.1"
                     /translation="MDSSDPTARKFESAIVAVSPLFDLVLSVGERISRIAEPVDYEYY
                     PVREDEEEESSGD"
     gene            275..>1081
                     /locus_tag="P5138_11715"
     CDS             275..>1081
                     /locus_tag="P5138_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61290.1"
                     /translation="MITKNEILEREKRWTLIAGIASVMGVAIILATYGESSAAVRAAS
                     GFADRLREIDGDHSSLIMASIGQFVGWSLLAVPLVFLFRAAAARAPQVRGALVGVMVI
                     APVLIGLGSIISAGSIIEAASDFEKEADQAAITKCVSDKVADESDSASGDESAGQTGT
                     TGDSGTTGGSGGSVSEQQRSDFEQECEDDEARDVRAATSLAPLETGVGLAGLLGFTVA
                     VIYVSLWSMRTGLLSRFWGSLGIALGVVFAFFTLFTLIWFIYVGLLLAGWV"
BASE COUNT          166 a          331 c          374 g          210 t
ORIGIN      
        1 ggcgccggct tcgaggggga cgtgggggag gttcacgggc aaggtcgctg tagcgtattg
       61 accgatggac tcctccgacc ccaccgccag gaagttcgaa tccgcgatag tcgcggtttc
      121 gccccttttc gatctcgtcc tttcggtcgg agagcggatc tcgcgaatcg ccgagccggt
      181 cgactacgag tactacccgg tgcgcgaaga cgaggaagaa gaaagctccg gggactgagc
      241 ggcgtatagg cttgcccggc ctccgggctc aagcatgatc acgaagaacg agatactcga
      301 gcgcgagaag cgctggaccc tgatcgccgg catcgcctcg gtgatgggtg tggcgatcat
      361 cctcgcgacc tacggcgagt catcggctgc ggtgagagct gcgtcgggtt tcgccgaccg
      421 gctgcgggaa atcgacggtg accactcgag cctgatcatg gccagcatcg gccagttcgt
      481 cggctggtcc ctgctggcgg tgccgctggt gttcctcttc cgggccgcag cggcccgggc
      541 cccgcaggtg cgtggtgccc tggtcggggt gatggtcatc gctccggtcc tgatcggtct
      601 cggcagcatc atcagcgccg gttcgatcat tgaggcggca tccgatttcg agaaggaagc
      661 tgatcaggcg gcgatcacaa agtgtgtgag cgacaaggtc gccgacgagt ccgacagtgc
      721 cagcggagac gaatcggcgg gccagaccgg caccaccggt gactcgggaa cgaccggcgg
      781 cagcggtggc tccgtctcgg agcagcaacg ctccgacttc gagcaggaat gcgaagacga
      841 cgaggcccgt gacgtccgtg cggccacttc gctggcgccg ctggaaaccg gtgtcgggct
      901 ggccggcctg ctcggtttca cggtcgcggt gatctacgtc tccctctggt cgatgcgtac
      961 cggcctgctt tcccgcttct ggggttcgct cggtatcgct ctcggtgtgg tcttcgcgtt
     1021 cttcacgctc ttcaccctga tctggttcat ctacgtcggg ctgctcctgg ccggttgggt
     1081 g
//
LOCUS       DAOTIP010000747         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_164434_length_1081_cov_1.943470, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000747 DAOTIP010000000
VERSION     DAOTIP010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164434_length_1081_cov_1.943470"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1081)
                     /locus_tag="P5138_11720"
     CDS             complement(<1..>1081)
                     /locus_tag="P5138_11720"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRV61291.1"
                     /translation="ALWADEGLPSGGEALLAPPLSRLRKALGPGKLEGRSELGLSLGD
                     EAWIDWEAAEEGIEKARNLEDEGRHREAFDEAVAAEKILAGGLLPGLEAGWIDDYRNE
                     LEDLRLQALELTARAGGPLGDAERARGERAARQAVDAAPFRESARTALIEVMEAQGNI
                     AEALRAYEDFRVLLRDELGTFPAPELSAVHERLLNAHENAVTADDKESPPAKAAAPES
                     EATREEGATLSETAAGPVPIERQATPEQIGRLIDPRITEIDLVGREEILRQLNGELDL
                     AVNGDLRIALLAGEGGIGKTRIAAELAAGRDDVTVLYGRSEPDEIRPFRIWSGLLRSA
                     MRQVRDIPPSEIVGADGPTLARILP"
BASE COUNT          135 a          395 c          354 g          197 t
ORIGIN      
        1 tccggcagga tccgggccag ggtgggaccg tcggcgccga cgatctcgga cggcggaatg
       61 tcccgcacct gccgcatcgc ggagcgaagc agtcccgacc agatccggaa gggcctgatc
      121 tcgtccggct cggatcgtcc gtagaggact gtcacgtcat cccggccggc ggccagttcg
      181 gccgcgatcc gcgtcttgcc gattccgccc tcgccggcca gcagggcaat tctcaggtcc
      241 ccgtttaccg caaggtcgag ttccccgttc agctgccgga ggatttcctc tcggccgacc
      301 aggtcgattt cggtgatcct gggatcgatc agccggccga tctgttccgg ggtggcctga
      361 cgctcaatcg gaaccgggcc tgctgccgtt tcggacaggg tcgccccttc ttcccgagtg
      421 gcttcgctct ccggcgcggc ggccttagcc gggggcgatt ccttgtcgtc ggcggtgacc
      481 gcgttctcgt gggcgttcag caggcgttcg tgaacggctg acagttcggg cgccgggaag
      541 gttccgagtt cgtcccggag caggacccgg aaatcctcgt aggcacgcag ggcttcggcg
      601 atattgccct gggcttccat cacttcgatc agcgcggttc gggccgactc cctgaacggg
      661 gcggcgtcga cggcctggcg cgcggctcgc tcgccccggg ccctttcggc gtcaccgagt
      721 ggcccgcccg cgcgcgcggt cagctccaga gcctgcagcc gcaggtcctc cagttcgttc
      781 cggtaatcgt cgatccagcc ggcttcgagg ccgggaagca gcccgcccgc aaggatcttc
      841 tcggccgcaa ccgcttcgtc gaaagcctct cggtgccggc cttcgtcctc gaggttccgg
      901 gccttctcga tcccctcctc ggcggcttcc cagtcgatcc aggcttcgtc acccaggctg
      961 aggccgagct ccgagcgtcc ctccagcttg cccgggccga gagctttcct cagccgcgac
     1021 agcggcggcg ccagcagcgc ctcgcctccg ctcggcaggc cctcgtccgc ccagagtgcc
     1081 a
//
LOCUS       DAOTIP010000748         1075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_165746_length_1075_cov_2.335294, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000748 DAOTIP010000000
VERSION     DAOTIP010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165746_length_1075_cov_2.335294"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /locus_tag="P5138_11725"
     CDS             complement(<1..>1075)
                     /locus_tag="P5138_11725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV61292.1"
                     /translation="PEGEVSETHDAGSLEDPELKSFRSGFWSTVEIPATGPGKVKIEA
                     ALKLADGTVRTAPLAEIEMVAPESFEAGCDSPPEARVVICMATHNPDIGLLRIQLDSL
                     RSQTVTDWVCLISDDCSLPDTYRQIEAEIEGDDRFFISRSEARLGFYRNFERALGMVP
                     ESAELVALCDQDDRWYPEKLAELEKGLGKNQLVYSDQRLVTEDGQVIAETYWSSRRNN
                     HTNFASMLIANTVTGAASLFTREMLEYALPFPEAPGEQFHDHWLALVAMSTGGIGYVD
                     RPLYDYVQHGSAVLGHDAANAGIKAGRPKRPKHLSIGAVRQFFSGWRSAYFNAYLRLS
                     VLARVILARCGERLTGRKRRTLER"
BASE COUNT          187 a          351 c          349 g          188 t
ORIGIN      
        1 accgctccag ggtccggcgc ttgcggccag tgagcctttc accgcaacgg gcgaggatca
       61 cccgggccag caccgaaaga cgcaggtaag cgttgaaata agccgaacgc cagccggaga
      121 agaactgccg caccgcgccg atgctcaggt gcttcggccg tttcggccgg ccggctttga
      181 tccccgcgtt cgccgcgtca tgcccgagca cggccgagcc gtgctggacg tagtcataga
      241 gcggacggtc cacatagccg atgccaccgg tggacatcgc gaccagggcc agccagtgat
      301 cgtggaactg ctcgcccggc gcctccggga aaggcagcgc gtattcgagc atctcacgcg
      361 tgaacagcga agctgccccg gtgaccgtgt tggcgatcag catcgaggcg aagttggtgt
      421 ggttgttgcg gcgagaagac cagtaggtct cggcgatcac ctgtccgtcc tcggtgacaa
      481 gacgctggtc cgagtagacc agctggttct tgcccaggcc cttttcgagc tcggccagct
      541 tctccgggta ccaacggtcg tcctggtcac agagcgcgac cagctcggcc gattcgggaa
      601 ccattcccag ggcccgttcg aagttccggt agaagccgag cctcgcctcc gaacgggaga
      661 tgaagaagcg gtcgtcgccc tcgatctcgg cttcgatctg gcggtaggta tccggcagcg
      721 agcagtcgtc gctgatcagg cagacccagt cggtcacggt ctggctgcgc agcgaatcga
      781 gctggatcct caagagcccg atgtccggat tgtgggtcgc catacagatc acgacccgcg
      841 cctccggggg tgaatcgcac ccagcctcga atgactccgg ggcgaccatt tcgatctcgg
      901 cgagcggggc cgtgcggacc gtgccgtcgg cgagtttcag cgccgcctcg atcttcacct
      961 ttccgggtcc ggtggcgggg atctcgaccg tggaccagaa gccgctccgg aaactcttca
     1021 gttccgggtc ctcgagcgaa cctgcgtcat gggtctccga gacctcccct tccgg
//
LOCUS       DAOTIP010000749         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_166218_length_1073_cov_2.232809, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000749 DAOTIP010000000
VERSION     DAOTIP010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166218_length_1073_cov_2.232809"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..601)
                     /locus_tag="P5138_11730"
     CDS             complement(137..601)
                     /locus_tag="P5138_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61293.1"
                     /translation="MRTRSDNDRFQIDRSIFDQAFDHPDEQAEREAIRPLPEGWTVTA
                     DDIHGAITEAQEAGAVRCPENLDILVFPSSIDESDNCLIVVWSTLHDTSGYVAAGFTG
                     PRDCGNREIWEQDDPKPRTFAMIEDIVVLANRVIGQVRAVEKVIEAGRGNTR"
     gene            complement(598..>1073)
                     /locus_tag="P5138_11735"
     CDS             complement(598..>1073)
                     /locus_tag="P5138_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61294.1"
                     /translation="PSYVTSRFPGVRPLDDLTVTIKEDGLWEITTTLMDRAPDQRTGS
                     LVMRRVAANPKPEDTEVVLLGKAGVDTGYAAIVAEKFGRIDNWTEYYDRLSSIHLEAG
                     QIAHLDGTLVVTSGIGDGNFSVYGTRHRQTGELVRVTIDFTPSQDEWETVYGGAR"
BASE COUNT          170 a          318 c          332 g          253 t
ORIGIN      
        1 ctgtctgagt caatgtggtg gagcgtgtcg agcgggtaga tcccttcgaa tccggatccg
       61 cggtttccct gctcgagctt tgacttgaga agcacatggt cgatcgtgaa gagcggatct
      121 gtctggtcgg gcccgttcat cgggtgttcc ccctgccggc ttcgatcact ttctcgaccg
      181 cccggacctg tccgatcaca cggttggcca ggaccactat gtcttcgatc atcgcgaagg
      241 ttctgggttt cggatcatcc tgctcccaga tttcgcggtt gccgcagtcg cgtggcccgg
      301 tgaaccctgc ggcgacatac ccggaggtgt cgtgaagggt tgaccagacc acgatcaggc
      361 agttgtcgct ttcgtcgatc gaacttggga aaacgagaat gtccaggttt tcgggacaac
      421 ggaccgcccc ggcttcctgc gcctcggtga tcgctccgtg aatgtcatcg gcggtaacgg
      481 tccagccttc cgggagcggc cggatcgctt ctctttcggc ttgttcgtcg ggatggtcga
      541 aggcctggtc gaagatggac cggtcgatct ggaaccggtc gttgtcggat cgggttctca
      601 tcgggcacct ccgtaaaccg tctcccattc gtcctggctc ggggtgaaat caatcgtgac
      661 ccgaaccagc tctccggttt gccgatggcg ggttccatac acgctgaagt tgccgtcacc
      721 gataccgctt gtgacgacca gggttccgtc gagatgggcg atctgcccag cctctaggtg
      781 gatgcttgaa agacggtcgt aatactcggt ccagttgtcg atgcgtccga atttctcggc
      841 gacgatcgct gcgtatccgg tgtctacccc cgctttgccg agcaacacaa cttcggtgtc
      901 ctccggtttc gggttcgcgg cgacccttcg catgaccagg ctgccggttc gctggtccgg
      961 cgcccggtcc atcagggttg tggtgatctc ccacaatccg tcctccttga tggtgacggt
     1021 gagatcgtcc agcggcctaa cccccgggaa gcgggaggtg acgtagctgg gat
//
LOCUS       DAOTIP010000750         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_167853_length_1066_cov_2.220574, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000750 DAOTIP010000000
VERSION     DAOTIP010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167853_length_1066_cov_2.220574"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="P5138_11740"
     CDS             complement(<1..219)
                     /locus_tag="P5138_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV61295.1"
                     /translation="MLFSTKAEYGVRLMVELGRQPNTGPVSLNAIAEAERLPLSYLEH
                     LVAKLRQAGLVTSTRGAHGGYSLAYPAEE"
     gene            complement(239..928)
                     /locus_tag="P5138_11745"
     CDS             complement(239..928)
                     /locus_tag="P5138_11745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV61296.1"
                     /translation="MSPDQVAERVALGPGVEVLIRPIRPTDKHLLADGFAALSPESKF
                     QRFFAPVVRLSESDLVYLTEIDHHHHEALVAIDPDEGDLIGVTRYIRTSPYRDREAEV
                     AIVISDQWRGKGLGKALLSRLATRAREEGVTHFLAVILAENEGSIDLFENFAPKKTHV
                     AAGDPGQVEVRIDLPWSGEFSDSVLGYALRSAARGSHRFRTWRRMRRRMRRRPGREKP
                     AGKVREFGKTD"
     gene            complement(925..1056)
                     /locus_tag="P5138_11750"
     CDS             complement(925..1056)
                     /locus_tag="P5138_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRV61297.1"
                     /translation="MAGMLHLSEKTVENHRANAMKKLGMRDRVELVRYAIRQGLIEP"
BASE COUNT          176 a          343 c          358 g          189 t
ORIGIN      
        1 ctcctcggcc gggtaggcga ggctgtagcc gccgtgggcg ccgcgggtcg aagtgaccag
       61 accggcctgg cgaagcttcg ccaccaggtg ctccagatag gaaaggggca ggcgctcggc
      121 ttcggcgatc gcgttaagcg aaacggggcc ggtgttgggc tggcgaccga gctcgaccat
      181 cagtcgaact ccgtactcgg ctttggtcga gaagagcatc gatataaatc ctagagcact
      241 agtcggtttt gccgaattct cgcaccttgc cagcgggctt ttcccggccc ggccgacgcc
      301 tcatccggcg tctcatgcgc cgccaggttc ggaagcggtg gctgccgcgg gcggccgagc
      361 gcaaggcata gccgagcacc gaatccgaga attcgccact ccaggggagg tcgatgcgga
      421 cctcgacctg gccgggatca ccagccgcaa catgggtctt cttcggggcg aagttctcga
      481 agaggtcgat cgagccctcg ttttcggcga ggatcacagc caggaagtgg gtcacgccct
      541 cctcccgggc cctggtggcg agccgggaaa gcaacgcctt gccgagacct ttgccgcgcc
      601 actggtccga aatcacgatc gccacctcgg cctcgcggtc ccggtagggg gaggtgcgga
      661 tgtagcgggt cacgccgatc aggtctcctt cgtcggggtc gatcgccacc agcgcctcgt
      721 ggtggtgatg gtcgatctcg gtcaggtaca cgaggtccga ttcactcagg cgaacgaccg
      781 gcgcgaagaa ccgctggaac ttcgattccg ggctgagtgc ggcaaagccg tcggccagca
      841 ggtgcttgtc ggtcgggcgg atcggccgga tcagcacctc gaccccgggg ccgagcgcca
      901 cccgctcggc aacctgatcg gggctcatgg ttcgatcaac ccctgccgga tcgcgtaccg
      961 gaccagttcg acccggtccc gcatgccgag cttcttcatc gcgttggccc ggtggttttc
     1021 aacggtcttc tcggaaaggt ggagcatccc ggcgatttcc ctcgtc
//
LOCUS       DAOTIP010000751         1058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_169838_length_1058_cov_3.292124, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000751 DAOTIP010000000
VERSION     DAOTIP010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169838_length_1058_cov_3.292124"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="P5138_11755"
     CDS             complement(<1..527)
                     /locus_tag="P5138_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61298.1"
                     /translation="MFPLERRTASGGFRTPAGMMTSLLVLIVGALTFDVSIARSAVVA
                     HQAADVNPGPSSSDPDDMASFGGNLYFAGRMPGKGHELVRLHNGKPQLVADLNPGKDS
                     SSPRDFIRFKGMLYFSASTEASGRELWRTGGNTTEIVADLRPGSESSDPFLMTVLGDQ
                     LLFRAAAPHLAEQFT"
     gene            complement(671..>1058)
                     /locus_tag="P5138_11760"
     CDS             complement(671..>1058)
                     /locus_tag="P5138_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRV61299.1"
                     /translation="ADGDRVPLAKRARGDYRHALRMLPPSTPGWETPVQIASAAEGTG
                     LTEVWDSVEKHRAHLEETGQLEERRRHQTERWLDSMIEEAILGAVHRRSGVGEAIRSA
                     RAEVDAGEITVPQATLRVIEAAGLAD"
BASE COUNT          167 a          364 c          340 g          187 t
ORIGIN      
        1 tcggtgaact gctcggccag gtgcggcgca gcggccctga acaggagctg atcgccgagc
       61 acggtcatca ggaagggatc agaggattca gatccgggcc ggagatcagc cacaatctcg
      121 gtggtgttcc cgcccgttcg ccagagctct cggccggatg cttccgtcga agccgagaaa
      181 tagagcatgc ccttgaagcg gatgaagtcc cggggcgagg aggagtcctt gcccgggttg
      241 agatcggcca cgagctgcgg cttgccgttg tgaaggcgca cgagctcatg ccccttgccg
      301 ggcattcgac ccgcgaagta caggtttccg ccgaagctcg ccatgtcatc ggggtccgaa
      361 ctgctcgggc cggggttcac atctgcggcc tgatgagcca ccaccgccga acgggcgatc
      421 gagacgtcaa aggtcaacgc cccgacgatc agcacaagaa gcgaggtcat catgccggcc
      481 ggcgtgcgga atcctccgct tgcggtcctg cgttccaggg ggaacatcgt cctgccttcc
      541 tccgaacctc atcggccctg cctggccact tgtcgctaag tcgggttcgc acctccgata
      601 cttactcaat cgccggcgga tttccagtcg gattggagcg cccggcagtc tgaccgggtc
      661 gcgcgggcat tcagtcggcc agtccggcag cctcgatcac cctcagggtc gcctgcggga
      721 cggtgatctc cccggcatca acttcggccc ttgccgaccg gatcgcctcc ccgacgccgc
      781 ttcgccggtg gaccgcgccc agtatcgcct cctcgatcat cgaatcgagc cagcgttcgg
      841 tctgatggcg gcggcgctcc tcgagctgtc cggtctcctc gaggtgggca cggtgctttt
      901 cgaccgaatc ccagacttcc gtcagccccg tcccttctgc ggccgaggcg atctggaccg
      961 gggtctccca gccgggggtc gagggcggca gcatcctcag cgcatggcgg taatcgcccc
     1021 gggcccgctt ggccaggggc acccggtcac cgtccgcc
//
LOCUS       DAOTIP010000752         1054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_170850_length_1054_cov_2.438438, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000752 DAOTIP010000000
VERSION     DAOTIP010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170850_length_1054_cov_2.438438"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="P5138_11765"
     CDS             <1..439
                     /locus_tag="P5138_11765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRV61300.1"
                     /translation="HLDATTVLNRAISEKGIYPAVDPLDSTSRILKPDILGEDHYNTA
                     TEVQQVLQRYSELQQIIAILGIDELADEDKVLVYRARKIERFLSQPFFVAEQFTGTSG
                     EYVPVAETVRGFRMILDGELDDYAENAFYMKGSIDQVIEQGKG"
     gene            454..867
                     /gene="atpC"
                     /locus_tag="P5138_11770"
     CDS             454..867
                     /gene="atpC"
                     /locus_tag="P5138_11770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRV61301.1"
                     /translation="MAGESTFSVEVLTPEGEVFSGEAVQLSTRTVTGEIGILARHVPI
                     LAALKPTLLRLKVSESETREWAQSHGMLQVFANNAQILLEEAIEPGNLTVATLEEQKS
                     DAEARLADDSTGEAAREVAERDLERVTAFLEIAQA"
BASE COUNT          203 a          369 c          313 g          169 t
ORIGIN      
        1 ccacctcgac gcgacgaccg tgctcaaccg cgcgatctcg gagaagggca tctacccggc
       61 cgtcgatccg ctcgactcga cctcgcgcat cctcaagccg gacatcctcg gtgaggacca
      121 ctacaacacc gcgaccgagg tccagcaggt tctccagcgc tacagcgagc ttcagcagat
      181 cattgccatt ctcggtatcg acgaactggc cgacgaggac aaggtgctgg tgtaccgggc
      241 ccgcaagatt gagcgcttcc tctcgcagcc gttcttcgtg gccgagcagt tcaccggcac
      301 ctccggcgag tacgtgccgg tcgccgagac ggtccgaggc ttccgcatga tcctcgacgg
      361 tgaactggac gactacgccg agaacgcctt ctacatgaag ggctcgatcg accaggtcat
      421 cgagcagggc aagggctagg ggcttccggg aagttggccg gcgaaagtac attcagcgtt
      481 gaagtcctca cccccgaggg tgaggtcttc agcggcgagg ccgtccagct ctcgacccgg
      541 acggtcactg gcgagatcgg catcctggcc cgccacgtgc caattctcgc cgctctcaag
      601 ccgaccctgc tccgtctcaa ggtctcggaa tccgagacca gggagtgggc ccagtcccat
      661 ggaatgcttc aggtcttcgc caacaacgcc cagatcctgc tcgaggaagc gatcgagccg
      721 ggcaatctga ccgtggcgac gctcgaagag cagaagagcg acgccgaagc ccgcctcgcc
      781 gacgattcca ccggcgaggc tgcccgcgag gtcgccgagc gcgacctcga gcgggtgacc
      841 gccttcctcg agatcgccca ggcctagccc gggcggcgcc aggaaccacc agctgtccgc
      901 tggtcttcgt caacgccagg aaaaggcagc tcacgtaccc gcgtactatc acggccgttt
      961 cctggcttat tcctcgccag cgaacagctg gaggcccctg gcatccaccc gctgacgccc
     1021 gaggaacgac catccccaat attctgcccg gatg
//
LOCUS       DAOTIP010000753         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_171284_length_1052_cov_3.590772, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000753 DAOTIP010000000
VERSION     DAOTIP010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171284_length_1052_cov_3.590772"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="P5138_11775"
     CDS             complement(<1..946)
                     /locus_tag="P5138_11775"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRV61302.1"
                     /translation="MRTLLVLLLLPSLSWGAIDFAKDIRPVLEKKCLSCHNPNLSKGD
                     VSLAAASSVFAAGATLVVPGKALESDLYLMVMPEKPGEKPEMPAKGDPLTGVEAEALR
                     AWIDEGAKWPEGLVLREASKADKTWWAYQPLAKPKHDSIDDYLEDQLKENGLAMNPPA
                     DPRTLIRRVTYDLTGLPPTPEEVSAFVRDPDSKAYQKLVDRLLASPGYGERWGRHWLD
                     VVRFGESIGYERNVIIDNLWPFRDYVIRSINEDKPMNQFIREHLAGDVFGKDQPKVEI
                     GSAFLVAGPYDDVGNQDKAQAAQIRANTLDEIISATGEA"
BASE COUNT          180 a          316 c          335 g          221 t
ORIGIN      
        1 aggcctcgcc ggtggcgctg atgatttcat cgagcgtgtt cgcccggatt tgggcggcct
       61 gggccttgtc ctggttcccc acgtcgtcat aggggccggc cacgaggaag gcactgccga
      121 tttcgacctt cggctgatcc ttcccaaaga catcgcccgc gaggtgttcg cggatgaatt
      181 ggttcatcgg cttgtcttcg ttgatggacc ggatcacata gtcccggaag ggccagaggt
      241 tgtcgatgat gacgttgcgc tcatagccga tgctctcgcc gaagcgcacc acatctagcc
      301 agtgacgccc ccagcgttcg ccataacctg gagacgcgag caggcggtcg acgagctttt
      361 gataggcttt cgaatccgga tcgcggacaa aggcactgac ttcctccgga gtcggcggga
      421 ggcccgtgag atcataggtc acccggcgga tcagcgtccg cggatcggcc ggcggattca
      481 tcgcgaggcc gttctccttc agttgatctt cgaggtaatc gtcaatcgaa tcgtgtttcg
      541 gtttcgcgag gggctggtag gcccaccagg tcttgtccgc tttcgatgcc tcgcgcagga
      601 cgaggccctc gggccacttc gctccctcgt cgatccaggc gcgcagcgct tccgcctcga
      661 ccccggtgag cgggtcacct tttgccggca tttcgggttt ttcgcccggt ttctccggca
      721 tgaccatcaa atagagatcg ctttcgagcg ctttgcccgg caccaccagc gtcgcgcccg
      781 cggcaaaaac ggacgacgct gcagccagcg aaacgtcccc tttggaaagg ttcggattgt
      841 ggcagctgag gcatttcttt tccaggacgg gcctgatgtc ctttgcaaag tcgatcgcac
      901 cccaggagag ggatgggagt agcaggagga cgagcagtgt tctcatgggg tcaggccagg
      961 atttcccgca cgacttcgcc gtagacatcc gtcaggcgga agtcgcggcc cgcgtagcgg
     1021 taagtgagcc gggtgtggtc gaggcccagc ag
//
LOCUS       DAOTIP010000754         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_171396_length_1052_cov_2.227683, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000754 DAOTIP010000000
VERSION     DAOTIP010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171396_length_1052_cov_2.227683"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="P5138_11780"
     CDS             <1..283
                     /locus_tag="P5138_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61303.1"
                     /translation="LCGKPAVGVPPVLVSVINRRIGNYLGSADLYRDGELLLRYGRGC
                     DNRRLREEIGYELAFDSEGTARDFARTSQGIQALFPSPHPGSPVRSVSR"
     gene            280..>1052
                     /locus_tag="P5138_11785"
     CDS             280..>1052
                     /locus_tag="P5138_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV61304.1"
                     /translation="MSRPVAPSPEAVLEWASTVQSTSDLILRRLAGEYHEDAWGFDEE
                     FTEAVFPIFEFLYSTWWRVKVEGAANVPGHGRALLVANHSGAIFPFDGAMIATAIQKE
                     HPLPRWVRAMVLNWAFDLPFVSYILRRLGGVAANPANLGRLLNEDQPVMVFPEGQKGF
                     GKGFADRYRLQRFGRGGFIEVALETGSPIVPVAVVGAEEIYPKIGESKPLARAIGSPY
                     VPITPTFPWLGPLGLLPLPSRWRIEFLEPIDLSGYGPGS"
BASE COUNT          151 a          339 c          353 g          209 t
ORIGIN      
        1 gctctgcggc aagcccgcgg tcggtgtgcc gcccgtactg gtcagtgtga tcaatcgccg
       61 catcggcaat tacctgggca gcgccgacct ctaccgtgat ggcgagctcc tgctccgcta
      121 cggccgcggt tgtgacaacc ggcggttgcg agaggagatc ggttacgagc tggccttcga
      181 ttccgagggg acggcccggg atttcgcccg cacctcccag ggaattcagg ccctgtttcc
      241 ctctccccat ccgggttccc ccgtgaggag cgtgtccagg tgagccgtcc cgtcgccccg
      301 tcgccggaag ccgttctcga gtgggcctcc acggtccagt cgacttcgga cctgatcctc
      361 cggcgcctcg ccggggaata ccacgaggat gcctgggggt tcgacgaaga gttcaccgag
      421 gcggttttcc cgatcttcga gtttctctac tcgacctggt ggagggtgaa ggtcgaaggc
      481 gcggccaacg ttcccggtca cggccgggca ctgctggtgg ccaatcactc gggtgcgatc
      541 ttccccttcg acggcgcgat gatcgccacc gccatacaga aggagcatcc gctgccccgc
      601 tgggtcaggg cgatggtcct gaactgggca tttgaccttc cttttgtgag ctacatcctg
      661 aggcggctcg gcggggtcgc ggccaacccg gccaatcttg gtcggctgct gaacgaggat
      721 cagccggtca tggtctttcc ggagggtcag aagggcttcg gcaagggctt cgccgaccgc
      781 taccggttgc agcgattcgg aaggggcggc ttcatcgagg tggctctcga gaccggaagc
      841 ccgatcgtgc ccgtcgcggt cgtcggcgcg gaggagatct atcccaagat cggggagtcg
      901 aagcctctgg cccgggcgat cggttcgccc tacgtcccga tcacgccgac ttttccctgg
      961 ctggggccgt tgggcctgct tccgctgcct tcgaggtggc ggatcgagtt tctcgagccg
     1021 atcgaccttt ccgggtacgg gccgggctca ag
//
LOCUS       DAOTIP010000755         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_171544_length_1051_cov_3.502008, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000755 DAOTIP010000000
VERSION     DAOTIP010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171544_length_1051_cov_3.502008"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..656
                     /locus_tag="P5138_11790"
     CDS             132..656
                     /locus_tag="P5138_11790"
                     /inference="COORDINATES: protein motif:HMM:NF022728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3085 domain-containing protein"
                     /protein_id="HRV61305.1"
                     /translation="MRLSFDADHLRSLLEFSRAGTQRVPTFEQVLDPALWSDDLPDAR
                     IALLEAEIAEHGIALSARSEDLDPSLIGPGLILVGDEGVYLMANVPIETVVSAGVPHV
                     AYAAEVNPRMLPFDTWWEAKRTSFGPDDGTIFLPDEMIAGPLSRIPSGGAFQLEVSPE
                     QIGVLEPREPGPEI"
BASE COUNT          152 a          349 c          361 g          189 t
ORIGIN      
        1 cgcgccctcg ccggcggcga cctgcccgcc gccgagcggc tgttcctggc ggccatggac
       61 cgggtcttca aggacgggct cgcggtcgat cgggactggc tcgccgccca gccgggcttc
      121 gaggttcgcg catgaggctg agctttgatg cggatcacct gcgcagcctg ctcgagttca
      181 gccgggcggg cacgcagcgg gtcccgacgt tcgagcaggt actcgacccc gccctctggt
      241 cggacgacct gccggacgcc cggattgccc tcctcgaggc ggagatcgcg gagcacggga
      301 tcgccctatc cgcccggtcc gaggacctgg acccgtccct gatcgggccc ggcctgatcc
      361 tggtcggcga tgagggggtc tacctgatgg cgaacgtccc gatcgagacg gtcgtctcgg
      421 ccggggtgcc gcatgtcgcc tacgcggccg aggtgaaccc gagaatgctt ccgttcgaca
      481 cctggtggga ggcaaagcgg acgagcttcg ggccggacga cgggaccatc tttctgcctg
      541 acgagatgat cgcggggccg ctctcccgta tcccgtcagg gggggccttc cagctcgaag
      601 tgagcccgga acagatcggg gtcctggagc cgcgcgagcc cggcccggaa atctgagcgt
      661 ttcgggaaga tccggggatt ctgttgccag gctccccggg gccccgcctg ccggtgcgac
      721 ccgtcaggac ttcaggtggt tcacccggac cgcttaagcg gcgagggcgc gaaccggcgc
      781 agcgttggtg ttggcaactc tacgtttcgg accgatgacg gtggtgcctc accgagcgaa
      841 agctggcctc tttgaacgtt cgtcgatcct gtttcggccc cagcagaaga gcaccgcccg
      901 ggtgatcttg tggtggagcc gccgggtacc gcccccgggt ccgagccgcc tattacaggt
      961 gcgtttatcg ccatatccct tgcgggcccg ctcatctagc gaaatccgga ttttctcgtc
     1021 aagctgattc ttcgcatagg ttgacatccg g
//
LOCUS       DAOTIP010000756         1047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_172595_length_1047_cov_3.716734, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000756 DAOTIP010000000
VERSION     DAOTIP010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172595_length_1047_cov_3.716734"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="P5138_11795"
     CDS             <1..702
                     /locus_tag="P5138_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV61306.1"
                     /translation="ETGPPHHELFVRMRDRNGSIILPGAFLPTAERHDLVQELDSWVI
                     RKAISTLAGLDAETCPRLQINLSGRTVGDPKLPGFVQSELERTGVDPANLIFEVTETS
                     AIGNMTRAQEFSARMNELGCGFALDDFGTGFASFYYLKHLAFDYVKIDGEFVQNLASD
                     PVNRLLVKALVEISRGMGKQTVAEQVEDARTLGMLREFGVDFVQGHFLGRPRPLERAD
                     LTDLPEIQLDTPVRS"
     gene            complement(706..>1047)
                     /locus_tag="P5138_11800"
     CDS             complement(706..>1047)
                     /locus_tag="P5138_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRV61307.1"
                     /translation="ARYLMALFDLGQEGSPPTQARLARELDVSQPSALQMVRRIREMG
                     LINEGDLGLTPAGMSAALVLTSRRTAATDLTSEVLGLDPDEARAEAEHLASSATPAFG
                     RKLVAWRKSRN"
BASE COUNT          184 a          340 c          346 g          177 t
ORIGIN      
        1 gagaccggtc cgccgcacca tgagctgttc gtgcggatga gggaccgcaa cggctcgatc
       61 atcctgccgg gcgccttcct gcccaccgct gaacgacatg atctggtcca ggaactggac
      121 tcgtgggtaa tccgcaaggc gatctccacc ttggccgggc tcgatgccga aacctgcccg
      181 cgccttcaga tcaacctgtc cgggcgcaca gtgggtgatc cgaagctgcc cggattcgtc
      241 cagagcgaac tcgagcggac cggggtcgac ccggcgaacc tgatcttcga ggtgaccgag
      301 accagcgcga tcgggaacat gacccgggcc caggaattct cggccaggat gaacgagctc
      361 ggctgcggct tcgcactcga tgacttcgga accggcttcg cctccttcta ctacctcaag
      421 cacctggcct tcgactacgt gaagatcgat ggcgaattcg tccagaacct tgcttcagac
      481 ccggtgaaca ggctgctggt gaaggccctg gtcgagatct cccgcgggat ggggaagcag
      541 acggtggccg aacaggtcga ggacgcccgg accctcggga tgctgcgcga gttcggcgtg
      601 gacttcgtcc agggtcactt cctgggtcgg ccccggccgc tggaacgggc cgacctgacc
      661 gatctccccg agatccagct ggacacgcca gttcggagct gagggtcagt tacgcgactt
      721 gcgccaggcg accagcttgc gaccgaaggc cggggttgcg ctggaagcca gatgctcggc
      781 ttcggcccgt gcctcgtccg ggtccaggcc caggacttca ctggtcaggt ccgtggctgc
      841 ggtgcgccgt gaagtgagaa caagcgccgc gctcattccg gcgggcgtga ggccgagatc
      901 cccttcgttg atcagaccca tctcgcggat ccgcctcacc atctgaaggg ccgagggctg
      961 agagacatcc agttcgcggg cgagccgcgc ctgggtcggg ggagagcctt cctgcccgag
     1021 gtcgaaaagc gccatcaggt acctggc
//
LOCUS       DAOTIP010000757         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_172894_length_1046_cov_2.881937, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000757 DAOTIP010000000
VERSION     DAOTIP010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172894_length_1046_cov_2.881937"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="P5138_11805"
     CDS             complement(<1..376)
                     /locus_tag="P5138_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRV61308.1"
                     /translation="MTPVMPSDFDPRNAEPEDFISPGLPVDEANTTGRLPIGDPEQIP
                     DPAEVEVPEDLREQIQQVVDKYPDKDHKGSPKSAAIPVLWIVQRRYGWCTPAGIVQAA
                     CVMQVTPAYLESVASFYDLLRTS"
     gene            complement(373..>1046)
                     /locus_tag="P5138_11810"
     CDS             complement(373..>1046)
                     /locus_tag="P5138_11810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570527.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRV61309.1"
                     /translation="EQKVRKLIEQLKTGIGQYEALLDKNEIFLIRTKDVGVVPKERLI
                     DLGVTGPLLRAAGEPWDLRKEGGYLAYPELDFDVPVGTVGDGYDRYRVRLTEMRESLK
                     IIEQILDGLPEGPWVADDRKYALPPRAELSTSMESLIHHFKLVTEGFRVEPGEVYNPI
                     ESPRGELGCFVMADGSSKPARVHFREPSFVNLQALADMTIGEYVADLIQTLAMLDPIL
                     GGIDR"
BASE COUNT          168 a          323 c          362 g          193 t
ORIGIN      
        1 ggctggtccg aagcaggtca tagaaggagg cgaccgattc caggtaggcg ggagtcacct
       61 gcatgacgca ggcagcctgg acgatccccg ccggggtaca ccagccgtag cggcgctgga
      121 cgatccagag caccgggatc gccgccgact tcgggctgcc cttgtgatcc ttgtccgggt
      181 acttgtcgac cacctgctgg atctgctcgc gcaggtcttc gggcacctcg acttcggccg
      241 ggtccggaat ctgctcggga tcgccgatcg gaaggcggcc ggtcgtgttg gcctcgtcga
      301 ccgggagccc gggcgagatg aaatcctcgg gttccgcgtt tcgcggatcg aaatcggagg
      361 gcatcacggg ggtcaccggt cgatgcctcc caggatcgga tcgagcatgg ccagggtctg
      421 gatcaggtcg gccacgtatt cgccgatcgt catgtcggcc agagcctgga ggttgacgaa
      481 agagggctcg cggaagtgga cccgggccgg cttcgaggag ccgtccgcca tgacgaagca
      541 gccgagttcg ccgcggggcg attcgatcgg gttgtagacc tcgcccggct cgacccggaa
      601 gccctcggtg accagtttga agtggtggat aagcgattcc atcgaggtcg agagctcggc
      661 ccttggcggc agcgcgtact ttcggtcatc cgcgacccag ggaccctcgg gcagcccgtc
      721 gaggatctgt tcgatgattt tcagcgactc gcgcatctcg gtcaggcgca cgcggtagcg
      781 gtcgtagccg tcgccgacgg ttccgaccgg cacgtcgaag tcgagttcgg gataggcgag
      841 gtatccgcct tccttgcgaa ggtcccaggg ttcgccggcg gcgcgaagca gcggcccggt
      901 cacgccgaga tcgatcagcc gttccttcgg gacgacaccg acgtccttgg tccggatcag
      961 gaagatctcg ttcttgtcga gcagcgcctc gtactggccg atgccggtct tcagctgctc
     1021 gatcagcttg cggaccttct gctcga
//
LOCUS       DAOTIP010000758         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_173159_length_1045_cov_3.019192, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000758 DAOTIP010000000
VERSION     DAOTIP010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173159_length_1045_cov_3.019192"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="P5138_11815"
     CDS             <1..298
                     /locus_tag="P5138_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRV61310.1"
                     /translation="PRSTVIVLGDARTNGRPPAEDAFARVADRANRTFWLNPEPELYW
                     NYGDSVMSAYTPFCDGAFECWKTEHLEQFVDVVASGKVEHMPSGTRKYTSGHRY"
     gene            306..554
                     /locus_tag="P5138_11820"
     CDS             306..554
                     /locus_tag="P5138_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRV61311.1"
                     /translation="MSARRIRVRLQPKASRNELKGWKEDPESGERILQARVTAPPVDG
                     KANKALIALLAKEFKTPKSKIRIVQGETSRDKLVELAG"
     gene            complement(543..>1045)
                     /locus_tag="P5138_11825"
     CDS             complement(543..>1045)
                     /locus_tag="P5138_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRV61312.1"
                     /translation="CADLPCPCSKAAGGGSGSGGEEAASEPPRSFSGTVVDVADGDTI
                     DVSTPSGSVETIRIIGIDTPEVYGGVECGGPAASAMMKRLATGQRVRVTSDPTQDRRD
                     HYGRLLAYIDKGSQDLGYTMVRRGLASAYPYDGPFQRYPKYARADRRARTHGIGSWSH
                     CDISPG"
BASE COUNT          182 a          348 c          354 g          161 t
ORIGIN      
        1 cccccgctcg actgtgatcg tgctcggtga tgcccgcacc aacggccggc cgccggccga
       61 agacgccttc gcccgggtgg ccgaccgggc caaccgcacc ttctggctca atcccgagcc
      121 ggagctctac tggaactacg gcgattcggt gatgagcgcc tacaccccgt tctgcgacgg
      181 cgccttcgaa tgctggaaga ccgagcatct cgagcagttc gtggacgtgg tcgcctccgg
      241 caaggtcgag cacatgccat caggcacccg caagtacacc tccggccacc gctactgacc
      301 gggccgtgag cgccagaaga atccgggtcc gactccagcc gaaggcgtcg aggaacgaac
      361 tgaagggctg gaaggaagac ccggagtcag gcgaaaggat ccttcaggcc cgggtcaccg
      421 ccccaccggt cgacggaaag gcaaacaagg ccctgatcgc cctcctcgcc aaggagttca
      481 agacccccaa gtcaaagatt cgcatagtcc agggagaaac ctcccgcgac aagctggtgg
      541 agctagccgg gtgagatgtc gcagtgggac cagcttccga tgccgtgggt ccgggcgcgg
      601 cggtcggctc tggcgtattt cgggtagcgc tggaaggggc cgtcgtaggg gtaggcggag
      661 gccagccccc gtctgaccat cgtgtagccg aggtcctgac tccccttgtc gatgtaggcg
      721 agcaggcggc cgtagtggtc gcggcggtcc tgggtcgggt cggaggtgac cctcacgcgc
      781 tggccggtgg cgagtcgttt catcatggcc gacgcggccg ggcctccgca ttcgacgccg
      841 ccgtagacct ccggggtgtc gatgccgatg atcctgatcg tctcgaccga accggagggg
      901 gtgctcacgt cgatcgtgtc accgtcggcg acgtcgacga ccgtgccact gaagctgcgg
      961 ggcggctcgg atgcggcctc ctccccgccg gaacccgatc ctccacccgc cgccttcgag
     1021 catggacagg ggagatcggc acagg
//
LOCUS       DAOTIP010000759         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_174008_length_1042_cov_2.492401, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000759 DAOTIP010000000
VERSION     DAOTIP010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174008_length_1042_cov_2.492401"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="P5138_11830"
     CDS             complement(<1..783)
                     /locus_tag="P5138_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV61313.1"
                     /translation="MSDAQYALTQDQLDFLEVIRQMVVEKVTPRAHDIDESGEFPQDI
                     REMFAEHDLFGLPFEEKHGGTGTGELMLCAAIEEIAKGCASSSLMVAAQDLSSLPMKL
                     AASDELKDRILPKMASGEWLGAFCLSEPDAGSDPGAMRTRATKVDGGWKLNGAKNWIT
                     NSGVADVYVTFAVTDPDVKFSRGITCFAVMGDAEGFRIDALEKKMGMKGSPTGQPVYE
                     DVFVPDEDVIGEVGKGFSVAMQTLDRSRLGIGAQALGIAQGAT"
     gene            complement(831..>1042)
                     /locus_tag="P5138_11835"
     CDS             complement(831..>1042)
                     /locus_tag="P5138_11835"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935221.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRV61314.1"
                     /translation="PLLVDEALMAEPVETEAKEHLDAFADAIEQILEEAESDPEIARN
                     APYTTPVRRLDEVGATRNPRIRQPQ"
BASE COUNT          174 a          334 c          331 g          203 t
ORIGIN      
        1 ggtcgcgccc tgggcgatgc ctagcgcctg ggcaccgatg ccgaggcggg agcggtcgag
       61 agtctgcatg gcgaccgaga agcccttgcc cacctcgccg atcacgtcct cgtcggggac
      121 gaacacgtcc tcgtagacgg gctggccggt cggggagccc ttcatgccca tcttcttctc
      181 gagcgcgtcg atgcggaagc cttcggcgtc ccccatcacg gcgaagcagg tgatgccgcg
      241 ggagaacttc acgtccggat cggtcacggc gaaggtgacg tatacgtcag ccacaccgga
      301 gttggtgatc cagttctttg ccccgttcag cttccagcca ccgtcaacct tggtcgcgcg
      361 ggtgcgcatt gcgccggggt cggagccggc gtccggctcg gagagacaaa aggcgccgag
      421 ccactcgccg gaagccatct tcggaaggat ccggtctttc agctcatcgg aggcggccag
      481 cttcatcggc agcgaggaga ggtcctgcgc ggcgaccatg agcgacgaag aggcgcagcc
      541 cttggcgatt tcctcgatcg cggcgcagag catcagctcg ccggtaccgg ttccgccatg
      601 cttctcctcg aagggcaggc cgaagaggtc atgctcggca aacatttccc ggatgtcctg
      661 cggaaattcg cctgactcgt cgatgtcatg ggcccgcggg gtgaccttct cgaccaccat
      721 ctgacggatc acctcgagga agtcgagctg gtcctgggtc agggcgtact gggcatcact
      781 cattcggtca ctccgcttcg ttcaagttca gaaaaactgc gacagacagc ctattgaggc
      841 tgccttattc tggggtttct ggtcgcgccc acttcgtcga gtcttctaac cggggtcgtg
      901 tagggcgcgt tgcgggcgat ctccgggtct gactcggctt cctccaggat ctgctcgatc
      961 gcatccgcga aagcgtccag atgttccttc gcctcggttt cgaccggctc ggccatcaac
     1021 gcctcgtcga ccagcaacgg ga
//
LOCUS       DAOTIP010000760         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_174217_length_1041_cov_2.961460, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000760 DAOTIP010000000
VERSION     DAOTIP010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174217_length_1041_cov_2.961460"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="P5138_11840"
     CDS             <1..326
                     /locus_tag="P5138_11840"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRV61315.1"
                     /translation="GAVSAGSYDAWLVVVRGGGLDAGTRFDVFGGVTLGRSAEADVSF
                     TDRYASGLHARLYPRGDRYFLEDMNSTNGTLLDGGAVTSEAEVRDGSMIEIGDTAFRF
                     ELDRP"
     gene            346..>1041
                     /locus_tag="P5138_11845"
     CDS             346..>1041
                     /locus_tag="P5138_11845"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRV61316.1"
                     /translation="MLRVSGHSERTDVGRQRQANEDSYLARAPLFVVADGMGGAQAGE
                     VASLTAIQAFQAGLPDGSPDASIERTIALANHNIYEQAHSDASLAGMGTTITVAAVDA
                     DTEQVYIGHVGDSRAYRLREGIIQRLTKDHSLVEEMRRRGQITEEQAEDHPQRSIITR
                     ALGPEPEVQADLTAVPSEPGDIFLLCSDGLTTMIGDEKIREILMGATTLDAAARTLVD
                     EANRAGGRDNITVV"
BASE COUNT          185 a          345 c          346 g          165 t
ORIGIN      
        1 tcggagcggt ctccgccggt tcctatgacg cctggctcgt cgtggtgcga gggggaggcc
       61 tcgacgccgg cacccgcttc gacgtcttcg gcggggtcac cctgggccgg tcggccgagg
      121 ccgacgtttc cttcaccgac cgctacgcat ccggccttca cgcaaggctc taccctcgcg
      181 gtgacagata cttcctcgaa gacatgaatt cgaccaatgg caccctgctc gacggcggcg
      241 cggttacatc cgaggccgaa gtgcgggatg gcagcatgat cgagattggt gacacggcct
      301 tcaggtttga actggatcgt ccatgagtag cttgagaaga ccacaatgct gagggtttcc
      361 ggacattcag aacggaccga cgtcggccgc cagcggcagg ccaacgagga ctcctacctg
      421 gcccgggcgc cgctcttcgt ggtcgccgac gggatgggcg gcgcccaggc gggggaagtc
      481 gcatcgctga ccgcgatcca ggctttccag gccggtctgc ccgacggcag cccggacgca
      541 tcgatcgagc ggaccatcgc tctcgccaac cacaacattt acgagcaggc tcattcggat
      601 gcttcgctgg ccgggatggg cacgacgatc acggtcgccg cggtcgatgc cgacaccgaa
      661 caggtgtaca tcggccatgt cggtgactcc cgcgcatacc ggctgaggga ggggatcatc
      721 cagcggctga ccaaggacca ctcgctggtc gaggagatgc gccgccgcgg ccagatcacc
      781 gaggaacagg ccgaggatca tccccagcga tcgatcatca cccgggccct cggtcccgag
      841 ccggaagtcc aggctgacct gactgcggtg ccttccgagc cgggcgacat cttcctgctc
      901 tgctccgacg gtctgaccac gatgatcggg gacgagaaga tccgcgagat cctgatgggg
      961 gccaccaccc tggacgctgc ggcgcggacc ctggtcgacg aggcgaaccg ggccggcggc
     1021 cgggacaaca tcaccgtggt g
//
LOCUS       DAOTIP010000761         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_174275_length_1041_cov_2.323529, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000761 DAOTIP010000000
VERSION     DAOTIP010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174275_length_1041_cov_2.323529"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..>1041)
                     /locus_tag="P5138_11850"
     CDS             complement(94..>1041)
                     /locus_tag="P5138_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRV61317.1"
                     /translation="IRQPAGFCGIVGLKPTYGAISRFGMIAFASSLDQCGPLTRDVTD
                     AALLLGILQGRDEMDSTSVGIEGGVSAPSAERLDGMTFGVPRGLIGEGMEAGVLARFE
                     ETLIRICELGGEIVDIELPHAGHAISAYYVLAPAEASTNLSRYDGVRYGLRASDEDLL
                     SMYEVTREEGFGPEVKRRIMLGTYALSSGYYDAYYGRAQRVRTRIVEDFDKAFGLVDF
                     VVTPTSPTVAFKLGEKTTDPWSMYLSDIFTVPISLAGVPAISIPAGLAEPDGGGPELP
                     VGFQIAAPAFGEQKLLEASYALEQAIGFDGSPGLEGGSP"
BASE COUNT          187 a          340 c          344 g          170 t
ORIGIN      
        1 cgccgaagga aagctcgcag ccgcagaaca tcttggtcct ggtggaaagc tggacgtgga
       61 tctccagccc gatcaccgct tcgtactcgg tgctcatggc gatccacctt ccagtccggg
      121 cgaaccatcg aacccgatcg cctgttccag ggcgtaggag gcttcgagca gcttctgctc
      181 gccgaaggcc ggagcggcga tctggaagcc gaccggcaac tcgggccctc ccccgtcggg
      241 ttccgccagt ccggccggta tcgagatcgc cgggactccg gccagcgaaa tcggcaccgt
      301 gaagatgtcg ctcagataca tcgaccacgg gtcggtggtc ttctctccca gcttgaaggc
      361 gacggtgggc gaggtcgggg tgaccacgaa gtcgaccagg ccgaaggcct tgtcgaaatc
      421 ctcgacgatc cgggtccgca cccgctgggc acggccgtaa taggcgtcgt agtagccgga
      481 ggaaagtgcg taggttccga gcatgatgcg gcgcttgact tccggtccga agccctcctc
      541 cctggtcacc tcgtacatcg agagcaggtc ctcgtcgctg gcccgaaggc cgtagcggac
      601 gccgtcgtac cgggagaggt tggtcgaagc ctcagccggg gccagaacgt agtaggcgga
      661 gatcgcgtgg ccggcatgcg gcaactcgat gtcgacgatc tcgccaccga gctcgcagat
      721 ccggatcagg gtctcctcga atcgggccag cacaccggct tccattccct caccgatcag
      781 tccccgggga accccgaagg tcatgccgtc gagccgctcg gcgctcgggg cgctgacacc
      841 gccctcgatc ccgaccgagg tcgagtccat ctcgtcgcgg ccctggagga ttcccagcag
      901 cagggccgcg tccgtgacat cacgggtgag cggtccgcat tggtcgagcg aggaggcgaa
      961 ggcgatcatg ccgaagcggg aaatcgcacc gtaggttggc ttcaggccga ctatgccgca
     1021 gaatccggcc ggctggcgaa t
//
LOCUS       DAOTIP010000762         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_176605_length_1032_cov_3.718526, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000762 DAOTIP010000000
VERSION     DAOTIP010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176605_length_1032_cov_3.718526"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="pheS"
                     /locus_tag="P5138_11855"
     CDS             <1..875
                     /gene="pheS"
                     /locus_tag="P5138_11855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRV61318.1"
                     /translation="KALEGLIEAKVEGLRNAELEKSLAGDRIDVSLPGSPARAIGHIH
                     PITRTRRMIEDVMVGLGYQVMEGPEVEHDYYNFTALNHPEGHPARMLQDTFYVEDEEH
                     VEQVLLRTHTSPMQVRAMEASPPPLFMIVPGKTYRRDSDATHSPMFHQVEGLAVGEGI
                     TLADLKGTLLAMLREIFGPDREIRMRPHFFPFTEPSVEFDVSCFRCGGSGSLDDGSRC
                     GLCKGIGWIELGGAGMVDPNVFGFVADAGYDPDSVTGFAFGFGIERIAMLRHGVGDLR
                     LFFDNDVRMLEQFS"
BASE COUNT          179 a          325 c          353 g          175 t
ORIGIN      
        1 ggaaggccct cgaggggctg atcgaggcga aggtcgaggg cctcaggaat gccgagctcg
       61 aaaagtcgct ggccggggac cggattgacg tcagcctgcc gggcagtccg gcccgggcga
      121 tcggccacat ccatccgata acccggaccc ggcggatgat cgaggacgtg atggtcgggc
      181 tcggatacca ggtgatggaa ggcccggagg tcgagcacga ctactacaac ttcacggccc
      241 tgaaccatcc cgaaggccat ccggcgcgga tgctccagga caccttctac gtcgaagacg
      301 aagagcacgt cgagcaggtg ctgctcagaa cccatacctc gccgatgcag gtgcgggcga
      361 tggaggcctc gccgccgccg ctgttcatga tcgtgccggg caagacctac cgtcgcgact
      421 cggacgccac ccacagcccg atgttccatc aggtggaagg gctggccgtg ggggagggca
      481 tcacgctggc cgacctcaag ggcaccctgc tggccatgct gagggagatc ttcggacctg
      541 accgcgagat ccgcatgcgc ccccacttct tccccttcac cgagccctcg gtcgaattcg
      601 acgtttcctg cttccgctgt ggcggttccg gctcgctcga tgacggctcg cgctgcggcc
      661 tctgcaaggg catcggctgg atcgaactgg gcggggccgg gatggtcgac ccgaatgtct
      721 tcggattcgt ggccgacgcc ggatatgacc cggactcggt gaccggattc gccttcggct
      781 tcgggatcga acggatcgcc atgctgaggc acggggtcgg tgacctccgc ctctttttcg
      841 acaacgacgt gcggatgctg gagcagttct catgagagtc ccctacggat ggatggccga
      901 ctactgcgac ccgggtctgg aaccgcggga tctggccgag aagatggcga tgaccggtac
      961 cgaggtggag cgggtcgtca cgctgggccc gccctcgccc gaaggattcg tgatcggcaa
     1021 ggtgcttgcg gc
//
LOCUS       DAOTIP010000763         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_176641_length_1032_cov_2.771750, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000763 DAOTIP010000000
VERSION     DAOTIP010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176641_length_1032_cov_2.771750"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1032)
                     /locus_tag="P5138_11860"
     CDS             complement(<1..>1032)
                     /locus_tag="P5138_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV61319.1"
                     /translation="TAQKPLAVIEAMDAYYRGHNANVHRGVHTLSEEATDLYEGARAK
                     VAGLLGSTSPREVVFTRNVTSALNLVAQSWGTANLQAGDRILLTEMEHHANIVPWHMI
                     AQRTGAELQFAPIDEEGRLDRAALSELLAGGPRVFAFTHVSNVLGTKNPVTELVAEAK
                     AAGALTVIDGAQSVPKFPVNVAEIGADFYGLTAHKLYGPTGIGAIWGRRELWEALEPF
                     EGGGSMINKVTPEKITWASVPARFEAGTPPIAEAAGLAAAIDWVQEVGIRAIEAHENQ
                     LTAYALPRLAEVPGLTVFGPQDTVEREGIISFELEGVHPHDVSEILDRHGVAVRAGHH
                     CAQILMRRL"
BASE COUNT          153 a          337 c          370 g          172 t
ORIGIN      
        1 cgaggcggcg catcaggatc tgggcgcagt gatgaccggc ccgcaccgcg actccgtgcc
       61 ggtcgaggat ctcggagacg tcatgggggt ggactccctc cagttcgaag gagatgatgc
      121 cctcacgctc gaccgtgtcc tgcgggccga aaaccgtcag cccgggaacc tcggcaaggc
      181 ggggcagggc gtaggcggtg agctggttct cgtgggcctc gatcgcccgg atcccgacct
      241 cctgaaccca gtcgatcgcg gcggcaagcc cggccgcctc ggcgatcggc ggggtgccgg
      301 cctcgaacct cgccgggacc gaagcccagg tgatcttctc cggggtgacc ttgttgatca
      361 tcgagccgcc gccctcgaaa ggctccagcg cctcccagag ttcgcggcgg ccccagatcg
      421 ctccgatccc ggtcgggccg taaagcttgt gagcggttaa tccgtagaaa tcggcaccga
      481 tctcggccac gttgaccggg aacttcggaa ccgactgggc gccatcgatc acggtgagtg
      541 ccccggccgc cttcgcctcg gcgaccagtt cggtcaccgg gttcttggtg ccgagtacgt
      601 tcgagacatg ggtgaaggcg aagaccctgg ggccaccggc cagcagttcc gatagcgcgg
      661 cccggtcaag ccggccctcc tcgtcgatcg gcgcgaactg aagctcggca ccggtccgct
      721 gggcgatcat gtgccagggc acgatgtttg cgtggtgctc catctcggtc agcaggatgc
      781 ggtcgcccgc ctggaggttg gccgtgcccc aggactgggc gacgaggttg agggccgagg
      841 tcacattgcg ggtgaagacc acctcgcgcg gggaggtcga tccgagcagc ccggcgacct
      901 ttgcgcgcgc gccctcgtag aggtcggtgg cttcctcgga gagggtgtgg acgccgcggt
      961 ggacgttggc gttgtgcccg cggtagtagg cgtccatcgc ctcgatcacc gccagcggct
     1021 tctgggcggt gg
//
LOCUS       DAOTIP010000764         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_177178_length_1030_cov_3.033846, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000764 DAOTIP010000000
VERSION     DAOTIP010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177178_length_1030_cov_3.033846"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..>1030)
                     /locus_tag="P5138_11865"
     CDS             complement(82..>1030)
                     /locus_tag="P5138_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV61320.1"
                     /translation="GPALATGCTVVLKPAEQTPLSALRLGELIQEAGIPDGVVNIVPG
                     YGETAGAALAAHPDVDKVAFTGSTEIGKLIARTATGNLKRVSLELGGKSPNIIFSDAD
                     VDAAIEGAAQGIFFNQGEVCSAGSRLYVQSDQFDRVVEGISEAAKGIKVGHGLEEDTD
                     MGPLVSAEQFERVTGYLNRGAEEGNRAVTGGKAIDGPGFFVEPTVLVDAKPSDTIVRE
                     EVFGPVIAAMPFDDIDDLARQANDSHYGLAAGIWTRDISKAHRLASRIKAGTVHVNTY
                     HVFAAELPFGGYKQSGWGREMGEEVLKNYLETKSVIVGL"
BASE COUNT          154 a          343 c          347 g          186 t
ORIGIN      
        1 gagggagagg gccggctcat gggagagagc gccgaccctg tcttgatggt gggaggtgag
       61 acccgaccgt tgtcttacgg tctagagccc gacgatgacc gacttggtct cgaggtagtt
      121 cttcaggacc tcctcgccca tctcccgtcc ccagccgctc tgcttgtaac cgccgaacgg
      181 cagctctgcg gcgaacacgt ggtaggtgtt gacgtggacc gtgccggcct tgatccggct
      241 ggccaggcgg tgggccttgg agatgtcgcg ggtccagatg ccggctgcca gcccgtagtg
      301 ggaatcgttg gcctggcggg ccagatcgtc gatgtcatcg aacggcatcg ctgcgatgac
      361 cggtccgaag acctcctcgc ggacgatcgt gtcgctcggc ttcgcgtcca ccagcacggt
      421 cggttcgacg aagaagccgg gtccgtcaat cgccttgcca ccggtcaccg cccggttgcc
      481 ttcctcggcc ccacggttca ggtagccggt cacccgctcg aactgctcgg ccgagaccag
      541 cgggcccatg tccgtgtctt cctcgagtcc gtggccgacc ttgatgcctt tggcggcctc
      601 ggagatccct tcgaccaccc ggtcgaactg atccgactgg acgtagaggc gggagcccgc
      661 cgagcagacc tctccctggt tgaagaagat cccctgggcg gcaccttcga tcgccgcgtc
      721 cacgtccgcg tccgagaaga tgatgttcgg cgacttgcca ccgagctcca gcgagacacg
      781 cttgaggttg ccggtcgcgg tgcgggcgat cagcttgccg atctcggtcg agccggtgaa
      841 ggcgaccttg tccacatccg ggtgggcggc cagcgcggca ccggcggtct cgccgtagcc
      901 gggcacgatg ttgacgacac cgtccgggat tccggcctcc tggatcagct cgccgagccg
      961 cagggcggaa agcggagtct gttcggccgg cttgagaacc accgtgcatc cggtcgccag
     1021 cgccgggccg
//
LOCUS       DAOTIP010000765         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_177188_length_1030_cov_2.827692, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000765 DAOTIP010000000
VERSION     DAOTIP010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177188_length_1030_cov_2.827692"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="P5138_11870"
     CDS             complement(<1..348)
                     /locus_tag="P5138_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61321.1"
                     /translation="MAFSLIQEPKYDATASLLFRQPKFGINIFGAETQNFTDTDETRQ
                     AATNLDLVNQGRVRDAAAKAMGNDFDAAKVDSEVEAGTSGDSDVITITATDPDPETAA
                     KLANTYAASVVAYR"
     gene            684..>1030
                     /locus_tag="P5138_11875"
     CDS             684..>1030
                     /locus_tag="P5138_11875"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61322.1"
                     /translation="MIDLHCHFLPRIDDGPDSVEGSLEMARVWVESGVNRIVATPHVN
                     LRHPNRAETIRAAHEEMTAALERESIGLKVQTGAEISASVAIDLPEDEIGQLTLGDSD
                     WLLIEPPTTSTPFG"
BASE COUNT          210 a          316 c          307 g          197 t
ORIGIN      
        1 ccggtaggca accacactcg ccgcgtaggt gttggccagc ttggccgcgg tctcggggtc
       61 cggatcggtc gccgtaatcg tgattacgtc ggaatccccg gaagtgcctg cttcgacttc
      121 cgagtcgacc tttgccgcgt cgaaatcatt ccccatcgcc tttgcggccg cgtcccgcac
      181 ccggccctgg ttgaccaggt ctaggttggt cgctgcctga cgggtttcgt ccgtatccgt
      241 gaagttctgg gtctccgcgc cgaaaatatt gatcccgaat ttcggctggc ggaagagcag
      301 cgacgccgtg gcgtcatatt tcggttcctg gatgagcgaa aaggccaaag cagcaagtcc
      361 agccacccca agtgcagatc aggattaact tggcgcgtcg gcgcaagacc cagaggaact
      421 ggcccggctg ggtgtcctgc gattcgtctt ctggaaagcg ctcttggctg gaaaaagagt
      481 cagaagtatt cgcggtcaag tcattcctcc attagacatt tgatcccctc cccaaaccac
      541 agggaacata cggggattcc ccctcttctt gccgagaagc atccggggga tcgtactgaa
      601 agcaaagctg tgagcgacgc ctgaccggag gctagactga cctcgccccg gaaagctcaa
      661 ccgccagatc cgagccgcaa acaatgatcg acctccattg ccactttctt ccccgaatcg
      721 atgacggacc cgattcggtg gagggctcgc tcgaaatggc ccgggtctgg gttgagtcgg
      781 gggtgaaccg gatcgtcgcc accccccatg tgaacctgcg acacccgaac cgggcggaga
      841 caatcagggc ggcccatgag gaaatgactg cggcgctgga gcgggaatcg atcgggctaa
      901 aagtccagac cggggccgag atttcagcca gtgtggcaat cgacctgccg gaagatgaaa
      961 tcggccagct cacactcggt gactcggact ggctgttgat cgagccgccg accacctcga
     1021 cccccttcgg
//
LOCUS       DAOTIP010000766         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_177428_length_1029_cov_3.303901, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000766 DAOTIP010000000
VERSION     DAOTIP010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177428_length_1029_cov_3.303901"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1029)
                     /locus_tag="P5138_11880"
     CDS             complement(<1..>1029)
                     /locus_tag="P5138_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV61323.1"
                     /translation="NWIGRSEGAEVTFRCESANLDFPVFTTRPDTLFGATFFVMAPEH
                     PELDRLIEGTEVEEEVREYVNSVARESVAERGDEGRPKTGVPLGHYVTNPVNGEEIPM
                     FVADYVLMDYGTGAIMAVPAHDQRDYEFARVFDLPVKRVIEGEPEEAGDENGLPYTGD
                     GPMTGSGRFDGMNNREAYGEIVDWLKLEGRGDSAVNYRLRDWLVSRQRYWGAPIPIIY
                     CDDCGMVPVPEDQLPVRLPEIDDYAPKGKSPLAAATDWVAASCPNCGAEAKRETDTMD
                     TFVDSSWYFLRYLDAQNPEVAWDRTAADHWMPVDQYIGGAEHAILHLMYARFFTKALK
                     DMGHVNVV"
BASE COUNT          153 a          340 c          340 g          196 t
ORIGIN      
        1 tccacgacgt tcacgtggcc catgtccttc agggccttgg tgaagaatcg tgcgtacatc
       61 aggtggagga tggcgtgctc ggccccgccg atgtactggt cgaccggcat ccagtgatcg
      121 gccgccgtgc gatcccaggc aacctcgggg ttctgcgcgt ccaggtacct caggaagtac
      181 caggaagaat cgacgaaggt gtccatggtg tccgtttcgc gcttcgcttc ggcgccgcaa
      241 ttcgggcagc tggcggcaac ccagtcggtg gccgcggcca gcgggctctt gcccttcggg
      301 gcgtagtcgt cgatctcggg caacctcacc ggaagctggt cctccggcac cggcaccatg
      361 ccgcagtcgt cgcagtagat gatcgggatc ggggcgcccc agtagcgctg gcgtgacacc
      421 agccagtcac gcagccggta gttgaccgcc gaatctccgc gtccctcgag cttcagccag
      481 tcgacgatct cgccgtaggc ctcgcggttg ttcattccgt cgaagcgtcc ggaaccggtc
      541 atcgggccgt ccccggtgta aggcaggccg ttctcgtctc ccgcctcctc cggctcccct
      601 tcgatcaccc gcttcaccgg gaggtcgaag acccgggcga actcgtagtc gcgctggtcg
      661 tgggccggca ccgccatgat tgccccggtg ccgtagtcca tcagcacgta gtcggcgacg
      721 aacatcggga tctcctcgcc gttgaccggg ttggtgacgt agtgaccgag cggcacgccg
      781 gttttcggcc ttccttcgtc gccgcgttcg gcgaccgatt cgcgggccac cgagttcacg
      841 tattcgcgga cctcctcctc gacctcggtg ccctcgatca gccggtcgag ttcggggtgc
      901 tccggggcca tcacgaagaa ggtggctccg aagagggtgt cggggcgggt ggtgaagacc
      961 gggaagtcga ggttggccga ttcgcagcgg aaggtgactt cagcgccttc cgagcggccg
     1021 atccagttc
//
LOCUS       DAOTIP010000767         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_179064_length_1023_cov_3.478306, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000767 DAOTIP010000000
VERSION     DAOTIP010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179064_length_1023_cov_3.478306"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="P5138_11885"
     CDS             <1..646
                     /locus_tag="P5138_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRV61324.1"
                     /translation="VGIIGATVMPHVIYLHSALTQDRIQPQNDKERHSLIDFQKLDVG
                     LAMGIAGFINMSMLAIAAAVMGGTGIDTIEGAFDGLKNLVSPAAGLAFGLALLASGIA
                     ASSVGTFAGQVVMQGFINRTIPLFLRRAITMAPALVVLAAGLDPTRSLVISQVVLSFG
                     IPFALVPLIMLTARRDVMGEGFENHRLTTIFASFFAVIIIALNLFLLYQTFTGS"
     gene            730..>1023
                     /locus_tag="P5138_11890"
     CDS             730..>1023
                     /locus_tag="P5138_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV61325.1"
                     /translation="MSDSDSHTERSRWIALYVLCAGVLMIVLDATVVNVALPAIQKDL
                     GFSQSGLAWVVNAYLIAFGGLLLLSGRLGDLISRRDVFITGLVLFTIASLLCGL"
BASE COUNT          156 a          369 c          282 g          216 t
ORIGIN      
        1 ggtcggaatc atcggggcga ccgtgatgcc ccatgtgatc tacctgcatt ccgccctcac
       61 ccaggaccgg atccagccgc aaaacgacaa ggaacggcat tcgctgatcg acttccagaa
      121 gctcgacgtc ggcctggcga tgggcatcgc cggcttcatc aacatgtcga tgctggcgat
      181 cgccgccgcc gtgatgggcg ggaccggcat cgacacgatc gagggcgcct tcgacggact
      241 caagaacctg gtcagcccgg ccgccggcct ggccttcggc ctggccctgc tggcctccgg
      301 aattgcggcc tccagcgtcg gcaccttcgc gggtcaggtc gtgatgcagg gatttatcaa
      361 ccgcaccatt cccctcttcc tgcgccgggc aatcaccatg gctcccgccc tggtcgtcct
      421 cgcggccggc ctcgacccga cccgttccct ggtcatcagc caggtggtcc tttccttcgg
      481 catacccttc gccctggtcc ccctgatcat gctcaccgcc agacgggatg tgatgggaga
      541 gggcttcgag aaccaccgcc tgaccacgat cttcgcttct ttcttcgcgg tgataatcat
      601 cgccctgaac ctcttcctgc tctaccagac cttcaccggg tcctagaaca ccaccggctg
      661 agccggccca tccgaattgt tagtgtggcc tgcttgaccg accagtctga aaacccagtc
      721 atttcgtcca tttcagactc cgatagccac accgaacgtt cgcgctggat cgcgctctac
      781 gttctctgcg ccggggttct gatgatcgtg ctcgacgcca ccgtcgtgaa cgtcgcgctg
      841 ccggcgatcc agaaggacct cggcttctcc cagtcgggcc tggcctgggt ggtgaacgcc
      901 tacctgatcg ccttcggcgg tctgttgctg ctctccggcc ggctcggcga cctgatctcg
      961 cgacgtgacg tcttcatcac cggcctcgtc ctcttcacca tcgcgtccct gctctgcggg
     1021 ctt
//
LOCUS       DAOTIP010000768         1016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_180982_length_1016_cov_3.143600, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000768 DAOTIP010000000
VERSION     DAOTIP010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180982_length_1016_cov_3.143600"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..>1016)
                     /locus_tag="P5138_11895"
     CDS             complement(227..>1016)
                     /locus_tag="P5138_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61326.1"
                     /translation="VSVENGGSSQIAARLLETKGGQERIIARGVYRPDKSGRQVIQLH
                     GNVYHFDQGTQARLQLLPRDGNPDLSALAYVRPSNDQQDVTVSNVEVRLPVREEPGSL
                     SGLVKSPLPKVLPKGYELADDYKSIGSVAPDGSPKPANKATVKGKTLKLKLGCGGVNL
                     CRTVKVEIKGTKKPLKNVLIAKKSGVKMTAGKTATISFKLTSKARKAFRDSKKRKVVR
                     KNGRKKVKIVKSKGLKKTGAKVTVRGWNPAKSTKLTIKRTGKVK"
BASE COUNT          140 a          319 c          338 g          219 t
ORIGIN      
        1 tgccaggagc caccttcccg gacccggccc aagagctcgg cgctgcagga acgcccgggg
       61 caggtcggct tttcgactcg acccgctgaa ggttggtggt ggggaggtac tcagcaggct
      121 tgtcaggtcc ggggcgggcg tacccggaaa agaccttcgg gggagcccga agggcgacgg
      181 tcttttccgg gtatgcccgt gccggaccca gccaatactg gcggggctac ttcaccttgc
      241 cggtgcgctt gatcgtgagc ttggtcgact tggctggatt ccagccgcgg acggtcacct
      301 tggcgccggt cttcttcagt cccttggact tgacgatctt gaccttcttg cggccgttct
      361 tgcgaaccac cttgcgcttc ttgctgtccc ggaaggcctt gcgtgccttc gaggtcagct
      421 tgaaggagat cgttgcggtc tttccggcgg tcatcttcac gccactcttc ttcgcgatca
      481 ggacgttctt caggggcttc ttcgttccct tgatttcgac cttgaccgtg cggcagaggt
      541 tgacgccgcc gcagcccagc ttgagcttca gggtcttgcc cttgaccgtg gccttgttgg
      601 ccggcttcgg tgaaccatcc ggcgcgaccg agccgatcga cttgtaatca tcggcgagct
      661 cgtagccctt cggcagcacc ttcggcagcg gtgacttcac gaggccgctg agcgagcccg
      721 gttcctcacg aaccggcagg cgcacttcca cgttgctgac cgtcacatcc tgctggtcat
      781 tggacggccg gacataggcc agggccgaaa ggtccgggtt gccgtcccgg ggcaggagct
      841 ggagccgggc ctgcgtgccc tggtcgaagt ggtagacgtt gccgtggagc tggatcacct
      901 ggcggccgct cttgtcgggg cggtagacgc cacgggcgat gatcctttcc tgcccgccct
      961 tggtttcgag caggcgggcg gcgatctggg atgagccgcc gttctcgacc gagacg
//
LOCUS       DAOTIP010000769         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_183959_length_1006_cov_2.600421, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000769 DAOTIP010000000
VERSION     DAOTIP010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183959_length_1006_cov_2.600421"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="P5138_11900"
     CDS             complement(<1..721)
                     /locus_tag="P5138_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV61327.1"
                     /translation="MNPVTGERAADRGVGFALEFEGLSYRYAGSDSVTLDRIDLSVQP
                     GEFIVLAGGSGSGKTSLLRAGCGLIPHFHGGELAGTALVAGRPVTENGPGELADVVGY
                     VAQDPETQIVSTTVAAELDLPLRFRGDHPAQRARAVEEVALALGIPHLLPRTVTTLSG
                     GELQRVALAVALVTRPPLILLDEPTSQLDPVSGDELIWLLRRLNEEWGVAVVMAEHRL
                     ERCLAAADRVVAMDSGRIGFDG"
     gene            complement(718..>1006)
                     /locus_tag="P5138_11905"
     CDS             complement(718..>1006)
                     /locus_tag="P5138_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV61328.1"
                     /translation="DRAVDVAATLEMRGYGRSGGTAGFHRARSRFDRRFWLAGTVLVS
                     LALLELVLGSDRFVTYPEISWSIGFEAPLAALAFLVGGFATWRPRVRGGRA"
BASE COUNT          187 a          370 c          329 g          120 t
ORIGIN      
        1 tgccgtcaaa gccgatccgc ccggaatcca tggcgacaac gcggtccgcc gcggcgaggc
       61 agcgttccag ccggtgctcg gccatgacca cagcgacccc ccactcctca ttcagtcgtc
      121 tgagcagcca gatcagctcg tcaccggaaa ccgggtcgag ctgggaggtc ggctcgtcca
      181 ggaggatcag cggcggccgg gtgaccaggg ccaccgccag cgctacccgc tggagctcac
      241 cgccggagag ggtcgtgacc gtgcgcggaa gcaggtgcgg gatccccagc gcaagcgcca
      301 cctcctccac ggcgcgcgcc cgctgagcgg gatggtcgcc ccggaagcga agcggcaggt
      361 cgagctccgc cgcgacggtg gtcgaaacga tctgggtttc cgggtcctgg gccacgtagc
      421 cgaccacatc ggccagttca cccggaccgt tctcggtcac cggcctgccg gccaccagcg
      481 ccgtcccggc cagctcgccc ccatggaaat gggggatcag gccgcagccc gcccgaagca
      541 gcgacgtctt tcccgaaccg gagccgccgg ccagcacgat gaactccccc ggctgaaccg
      601 aaaggtcgat ccggtcgagc gtcaccgaat cggaccccgc gtatcggtag ctcaggccct
      661 cgaactcgag cgcgaacccg acccctcggt cagccgcccg ttctccggtg accgggttca
      721 cgcccgacct ccacgaaccc gcgggcgcca ggtggcaaac cctccgacca ggaaggctaa
      781 ggcggcgaga ggagcttcga agccgatcga ccaggagatt tcagggtagg tcacgaaacg
      841 atccgaaccc agcacgagct cgagcagcgc aagtgacacg agcaccgttc cggcaagcca
      901 gaagcgacgg tcgaatcggg agcgtgcccg atggaaaccg gccgtgccgc cggaccgccc
      961 gtagccgcgc atctcaaggg tcgcggcgac gtcaaccgcc cggtcg
//
LOCUS       DAOTIP010000770         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_3022
            NODE_185070_length_1002_cov_2.865892, whole genome shotgun
            sequence.
ACCESSION   DAOTIP010000770 DAOTIP010000000
VERSION     DAOTIP010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868046
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:18:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,383
            CDSs (total)                      :: 2,347
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185070_length_1002_cov_2.865892"
                     /isolate="AMR_MDS_3022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="P5138_11910"
     CDS             <1..756
                     /locus_tag="P5138_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRV61329.1"
                     /translation="SDRWWIGRGPNGGYVAALVLRAMTVEVDAMIANSEEHDRRLPAR
                     SLSVHFLRPPTTGAAEIEVTVEHAGHVTVFLSARLIQDGEVKAKAMAVFSGEREGPAF
                     DKSEMPEAPVPEDLEEMDTEQAPVAVFGRYRAKFIKGVPGEAGEKAETMGWIRLRDEQ
                     PVDPVLAAAVLDVWYPAPFVAFEKLPFAPTLEYTVHFPRTIPEPGPPDWNLIRLTAEE
                     GVEGHFSEDAELWSRDGKLLAKARQMALLRHRK"
     gene            complement(758..>1002)
                     /locus_tag="P5138_11915"
     CDS             complement(758..>1002)
                     /locus_tag="P5138_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRV61330.1"
                     /translation="DAEDLRIAELRRRVEGGGIAEVILATNPTTTGEATASHIADLIR
                     EHTKVTRLAAGLPVGADLEHADEVTLGRAMSGRAVL"
BASE COUNT          182 a          324 c          340 g          156 t
ORIGIN      
        1 agcgacagat ggtggatcgg aagaggaccg aacggaggct acgtcgccgc attggtcctg
       61 cgggcgatga ccgtcgaagt cgacgcgatg attgcgaatt cggaggagca cgaccgacgg
      121 cttccggcga gatccctgag cgtccacttt ctccggccgc cgaccaccgg agccgccgag
      181 atcgaagtga ccgtcgagca cgccggacat gtgacggtct tcctttccgc ccgcctgatc
      241 caggacggtg aagtcaaggc caaggccatg gcggtcttct ccggggaacg ggaaggaccc
      301 gccttcgaca agtccgagat gccggaagca ccagttccag aagacctgga agagatggac
      361 accgaacagg cgccggtggc ggtcttcggc cgctaccggg cgaagttcat caagggagtt
      421 cccggcgaag ccggggaaaa ggctgaaacc atgggctgga tccgcctgcg ggacgagcaa
      481 ccggtcgacc cggtcctggc cgccgccgta ctagacgtct ggtacccggc gccgttcgtg
      541 gccttcgaga agctgccctt cgcccccacc ctcgagtaca cggtccactt cccgcggacg
      601 atcccggaac ccggcccgcc ggactggaac ctgatccgac tgaccgccga agaaggggtc
      661 gaagggcatt tctccgaaga tgccgagctc tggagccgag acggcaagct gctggccaag
      721 gccaggcaga tggcgctgct gcgccaccgc aagtaggtca gagaacggcc cggccggaca
      781 tcgcccggcc cagggtcact tcgtcggcgt gttcgaggtc ggcgccgact ggaaggccgg
      841 cggccaggcg agtgaccttg gtgtgctccc ggatcaggtc cgcgatgtgg gaggcggtgg
      901 cctcaccggt cgtggtcggg ttggtggcga ggatcacctc ggcgatcccc cctccttcca
      961 cccttctcct tagttccgcg atgcggagat cctctgcgtc ga
//
